LOCUS       DAOMXN010000001        72793 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_282_length_72793_cov_12.011370, whole genome shotgun sequence.
ACCESSION   DAOMXN010000001 DAOMXN010000000
VERSION     DAOMXN010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 72793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 72793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72793
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_282_length_72793_cov_12.011370"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="PK812_00005"
     CDS             <1..286
                     /locus_tag="PK812_00005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018260996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRJ67965.1"
                     /translation="GEGLPRTILEAAACGRGILTTDVPGCADFVRDGVEGRVLANGEA
                     DPFATALCDLAADPQAVIRMGESARSRVLAGYTEAAVGQAVAALYRDMLA"
     gene            283..942
                     /locus_tag="PK812_00010"
     CDS             283..942
                     /locus_tag="PK812_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRJ67966.1"
                     /translation="MSAAGPVRRETFIRAETKVLPVPHAPEIRLHLADEAIPLWEKTE
                     EQLAAIGLAPPFWAFAWAGGQALARYCLDHPDLVRRRRVLDFAAGSGLVGIAAAKAGA
                     GAVEAVEIDPFAVTAINLNARENDVTLSVRHADLIGQEGGWDVIFAGDVFYERDMAER
                     VFRWLDALHRRGAVVLIGDPGRSYLPKDRLEALATYQIPVTRALEDAEVKRTSVWRIR
                     G"
     gene            1028..1474
                     /locus_tag="PK812_00015"
     CDS             1028..1474
                     /locus_tag="PK812_00015"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HRJ67967.1"
                     /translation="MPVGERLRQAEILCTGRGTRLTAQRRVVLEALLTARRALGAYDL
                     IEQVSRATGKRTAPITIYRALEFLQEMGLAHRIESRNAFLACHGGHRPHALTVFMICD
                     CCGQVAEADAPGLEAELDRLATGHGFRAQSRVIEMAGRCSTCNEGP"
     gene            1471..2736
                     /gene="ispG"
                     /locus_tag="PK812_00020"
                     /gene_synonym="gcpE"
     CDS             1471..2736
                     /gene="ispG"
                     /locus_tag="PK812_00020"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HRJ67968.1"
                     /translation="MILQGKIVAEPAYGPLERHRTVGVRIGDGAGAVSVGGGAPIIVQ
                     SMTNTDTADIEATVRQVAALARAGSEVVRITVDRDEAAAAVPHIKERLLQRGITAPLV
                     GDFHYIGHKLLADHPACAEALDKYRINPGNVGFREKKDRQFGAIVEQAIRHGKAVRIG
                     ANWGSLDQELLTHLMDRNAASAQPRDARAITREAMVQSALLSAARAEEIGLAKDRIIL
                     SAKVSAVQDLITVYRMLAQRSDYALHLGLTEAGMGSKGLVASSAAMGILLQEGIGDTI
                     RYSLTPEPGGDRTAEVRAAQELLQTMGFRTFVPLVAACPGCGRTTSTVFQELARDIQD
                     WISTSMPEWKTRYPGVENLNVAVMGCIVNGPGESKHADIGISLPGTGETPSAPVFIDG
                     KKAATLKGAGIAAEFKAMVGTYIETRFGK"
     gene            complement(2782..3462)
                     /gene="purQ"
                     /locus_tag="PK812_00025"
     CDS             complement(2782..3462)
                     /gene="purQ"
                     /locus_tag="PK812_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003604527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HRJ67969.1"
                     /translation="MHAAVILFPGSNREGDVARALRLGGAKVSMAWHADSELPKGTDL
                     AVLPGGFSYGDYLRCGAIAARAPIMDAVRAHAARGGLVLGICNGFQILCESGLLPGIL
                     TRNAGLKFLCHMQHLKVGRTDTPFTKLYRRDQVIEVAIAHGEGNYTADAETLKRLEGE
                     DRVAFRYCDAAGDINEAANVNGSLNHIAGIYSEKLNVLGMMPHPENLVEAMMGGTDGL
                     PMFKGLAA"
     gene            complement(3462..3701)
                     /gene="purS"
                     /locus_tag="PK812_00030"
     CDS             complement(3462..3701)
                     /gene="purS"
                     /locus_tag="PK812_00030"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HRJ67970.1"
                     /translation="MKARVTVTLKTGVLDPQGKAIEGALKSLGVEGITSVRQGKVFDI
                     EVETQDKSRAEAILKQAGEKLLANTVVENFRVEVL"
     gene            complement(3856..4194)
                     /locus_tag="PK812_00035"
     CDS             complement(3856..4194)
                     /locus_tag="PK812_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HRJ67971.1"
                     /translation="MTYIVTDNCIKCKYMDCVEVCPVDCFYEGENMLVIHPDECIDCG
                     VCEPECPAEAIKPDTEPGLESWLKLNTDYSKSWPNITVKRESPADAKQFDGEKGKLEK
                     YFSPNPGQGD"
     gene            complement(4295..5218)
                     /locus_tag="PK812_00040"
     CDS             complement(4295..5218)
                     /locus_tag="PK812_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate/arogenate dehydrogenase family
                     protein"
                     /protein_id="HRJ67972.1"
                     /translation="MSARFSRLCLIGLGLIGSSIARAVREKDLAGTIVAVDASEFVRA
                     RVRELGLADEITDSPQAGVSGADGVILCVPVGANAAVGAAIGPCLKPGAIVSDVGSVK
                     AAVVRDVAPHLPANVHLVPAHPVAGTEFSGPDAGFASLFTNRWCILTPPEGQDEVAVA
                     KIRAFWEAIGSNVEVMTPQHHDLVLAITSHVPHLIAYNIVRTADDLETVTEGEVIKFS
                     AGGFRDFTRIAASDPTMWRDVFLHNREAVLEMVARFSEDLAQLARMVRWGDGDGLHAL
                     FTRTRAIRKSIIASGQETAAPDFGRSGPPKS"
     gene            complement(5215..6321)
                     /gene="hisC"
                     /locus_tag="PK812_00045"
     CDS             complement(5215..6321)
                     /gene="hisC"
                     /locus_tag="PK812_00045"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HRJ67973.1"
                     /translation="MSVPASTRPLPRKGVLDIEAYVPGKSAAPAGVKLHKLSSNENPL
                     GPSARAIEAYRACSEGLEYYPDGSATALRQAIAARYGLDPARIVCGAGSDEILNLLTH
                     AYVGPGDEGVFTEHGFLVYRIAITAAGGKPVVVKEKDFTADIDAILAAVTTRTRIVFL
                     ANPNNPTGTYLPFAEVKRLHAGLPPDVLLVLDAAYAEYVRRNDYSAGIELVSTSENVV
                     MCRTFSKIYGLAGLRIGWGYMPAHVADAINRIRGPFNLSIPAIAAGVAAIGDDAHVAA
                     TADHNERWLKALTDEITALGLTVTPSVGNFLLIHFASKAGKTAKDADAFLTQRGLILR
                     GVGAYGLPNALRLTVGSNESNRLVLQALRDFMAS"
     gene            complement(6345..7181)
                     /locus_tag="PK812_00050"
     CDS             complement(6345..7181)
                     /locus_tag="PK812_00050"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HRJ67974.1"
                     /translation="MPPKPTEDLGKLRAELDRIDAAMQALLIERSEVTDQVEQVKRRA
                     GTAGSAFRPGREAQIMRMLAERHRGSYPLDGTENIWRVIIATSTYTQVPFSVHGDVSG
                     GDAAMRDSVRFHFGFTVPFLPTPSAAAAIEAVDSASGDLGVFPRDQSIEAGAWWRLLH
                     GDNRPKIIARLPFIQRANHPSGMPVFVVAKPLKEAAVRDEIVASLWLERWRREVPEAL
                     RSVGVRLEASAGVESGLAVLVSHGGAVAPGAVLDALKSAGVVARYAEVGSHAAPYVHG
                     ES"
     gene            7466..8641
                     /locus_tag="PK812_00055"
     CDS             7466..8641
                     /locus_tag="PK812_00055"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HRJ67975.1"
                     /translation="MARKPTPAASRIADQANEPQSAVVRFGEDRPLRLESGVLFAPLQ
                     VAYQTYGALNATKSNAILICHALTGDQHVANVHPVTGKPGWWEDMIGPGRLMDTDRYF
                     VICANVLGACMGTSGPASLDPATGRPFGLDFPVVTIGDMVKAQEMLVTHLGIDQLLCV
                     IGGSMGGMQVLDWASRFPDRVFSAMPIATAAKHSAQNIAFHEVGRQSVMADPDWRGGR
                     YLLEGARPEKGLAVARMAAHITYLSERALQRKFGRNLQDRAAPTFSFDADFQVESYLR
                     YQGATFVERFDANSYLYLTRAMDYFDLAADHGGVLADAFRGARTRFCLISFTSDWLFP
                     TADSRQIVHALNAAGASVSFVEIESDKGHDAFLLDEPSLLATMKGFLEAAARAKGIA"
     gene            8638..9285
                     /gene="metW"
                     /locus_tag="PK812_00060"
     CDS             8638..9285
                     /gene="metW"
                     /locus_tag="PK812_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HRJ67976.1"
                     /translation="MSPDDMSLREQSAAMQARVDFLLVADMVESGARVLDVGCGDGEL
                     LALLAQSKQVDARGIELSREGVAGCVAKGLAVIQGDADTDLADYPNDCFDYVILSQTL
                     QATRNPRKVLEQMLRIGRRAIVSFPNFGHWRIRSGLFFRGRMPRTENLPYSWYDTPNI
                     HFCTIADFVELVGDLPATMEKAVALNASGSQLGFNAPWWLWNLVGEQAVFLLRRG"
     gene            complement(9293..10114)
                     /locus_tag="PK812_00065"
     CDS             complement(9293..10114)
                     /locus_tag="PK812_00065"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRJ67977.1"
                     /translation="MRRSILTIALFACVLILPVSAQSQTPAAPAAQAQASPPAWQPPK
                     RRRIVLMRHGDVSYFDASGQPTANADLVVLSDKGKAQADAAGRYLASIGAAKFDRIMS
                     SHLPRTIETAERVMAAGAIAGTLKQVPELREIRNGSTRSVATPDLPAVLLGIAQPRVP
                     ADMKFIGGETIGEMQARVYAAVDTLLQDETWDNALLVLHSVVNSAIVSRVLGGDGAFL
                     GRFESTAGCMHILDTAAKPGDWIVRAYNLCPNGAEYQGERLHTAEKLLIQALKGR"
     gene            10578..11147
                     /locus_tag="PK812_00070"
     CDS             10578..11147
                     /locus_tag="PK812_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HRJ67978.1"
                     /translation="MSAKKTTQREGFKTGEHIVYPAHGVGRIMAIEEQEIAGFKLELF
                     VVTFAKEKMTLRVPTSKIAAVGMRKLSGGAAIEKSLEILTGRARIKRTMWSRRAQEYE
                     AKINSGDLIATAEVVRDLYRSEAQPEQSYSERQLYETALARLLDEIAAVEQITETEAM
                     KRIDASLAKNPKRRAASEASDADAEEEAA"
     gene            11292..12164
                     /locus_tag="PK812_00075"
     CDS             11292..12164
                     /locus_tag="PK812_00075"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020179522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-32"
                     /protein_id="HRJ67979.1"
                     /translation="MSPLTGVRRQFVRAAMEAPYLSREEEHGLAVAWRDHKDRAALDR
                     LTQAHMRLAIALAAKFRNYGLPMADLIQEGHVGLMEAAARFEPDREVRFSTYASWWIR
                     AAIQDYVLKNWSIVRGGTSSAQKALFFNLRRLRAQIGLRISQGEQLDLHDEIAAAVGV
                     SRQDVVAMEARIGASDISLDAPVTEADGAGAAERMDFLVSESPGPDEAVSELMDSERR
                     AAWLSQALAQLSEREMHVVRTRRLVEDEDSATLESLGTTLGVSKERVRQIEKRALEKL
                     KAALVRLNPGMARG"
     gene            complement(12200..13558)
                     /locus_tag="PK812_00080"
     CDS             complement(12200..13558)
                     /locus_tag="PK812_00080"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HRJ67980.1"
                     /translation="MPASAPRVSGGETQAGREHQRLLQAFGGEYRAPAVQRILDDLSD
                     RLRAVTEKPAEKYRVTILNSAVVNAFALPSGNLYVTRGLLALANDLSEVASVLAHEIA
                     HVTARHAEGRAELEGRSVLVSRVRAEVLSNPGAAQLVRDQAQVAIASFSRQQELEADE
                     IGVRTLAGAGFDPHGAHRFLLALGRSSEFRERASEGQTRGEGPEFTATHPTTPDRIAA
                     ALAAARYFFEPGVIASERARWMQALNGMIYGEDSSEGFVRGRNFLHPKLGVAFTAPDG
                     FLLENSSQAVLGMTAGAQEALRLDSAKVAPDKKLEDFIVENRIENLTLSDVQTLTING
                     LPAATGIARGADWTFRIFAVRQGGTVYRLILASRSFTPATDERFFSAANSFRRLSQEE
                     QSRARPLRIAVVAAKRGETAEDFSQRMGGIDRPLERFLLLNGLARGAELVPGLTYKTV
                     VD"
     gene            complement(13705..14457)
                     /locus_tag="PK812_00085"
     CDS             complement(13705..14457)
                     /locus_tag="PK812_00085"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HRJ67981.1"
                     /translation="MALALVVLALPLQACPAPMGPASTVIAVAQTGEVSLADGRIVRL
                     SGIDWPSPDRQGWRDRLRMVLLRLAAGTQARVDSAGKSDRWGRLSGNLVLATPDGPPQ
                     PLWVQETLVALGLVRHWPEAATDPCRPRLLAAENAARAAGLGLWSPLSRRTMRVSRVV
                     ARQTLSGRAVFEARVRSVRTGRSATFVNFEGTRSATPFIMVRNRDLSRWRAEGLDPAT
                     FSGKRLRVRGVVSQVPTPRLHLTGPMAVEVLE"
     gene            14541..14996
                     /locus_tag="PK812_00090"
     CDS             14541..14996
                     /locus_tag="PK812_00090"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HRJ67982.1"
                     /translation="MTSNAPAAPLARYAAWKRRGDFIFMSGIIAVDPALGKVVMGYDD
                     IPVEAQALLGRTGEMSVDVKEGPILAQSWYVLDKIRQTIEGCGGSMGDVFKLVQYFRD
                     LRDYPHYNRVRKLFYPTDPPVSTVVEVSAMLPNEHILVEVEATAWLPLI"
     gene            15148..15825
                     /locus_tag="PK812_00095"
     CDS             15148..15825
                     /locus_tag="PK812_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family protein"
                     /protein_id="HRJ67983.1"
                     /translation="MKRVLLAAASAAALLGSQAFAADAPMKPGAMPIEAFNPWMVRLR
                     ALAVVPQEDASLSTAAGPIIGGKVGISNSVVPELDITYFFNRNFAVELILGTTPHKVK
                     GAGALAGVGPIGRAWLLPPTLTAQYHFYLTDSIKPYVGAGINYTIFYNEKSSGVFTGT
                     KFDLKDTFGLAVQAGVDIMINKNWGFNVDVKKIWLEPNVSVGSGAVTGKVKIDPWLIG
                     AGITYKF"
     gene            complement(15892..17214)
                     /locus_tag="PK812_00100"
     CDS             complement(15892..17214)
                     /locus_tag="PK812_00100"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine deaminase"
                     /protein_id="HRJ67984.1"
                     /translation="MTAGFAKVPHAARLRIVNATLPACLVQSGQLLLATPDGLARCDL
                     VLAEGKIASIVAAGVEPADGTTPTLDLDQGMILPCFADVHTHLDKGHIWPRRPNPDGT
                     FMGALNAVLADRQASWTAEDVAARMEFSLRCAYAHGTSAIRTHIDSRPGQTAISWPVF
                     AAMRETWAGRIALDASPLFGIELALDDAHMAEVSDMVDRHGHVMGAVTYMGPALRPGL
                     ERLFLLAREKGWDLDFHVDETLDPTAESLRVIAEMALSLWFPGRILVGHCCSLSMQAE
                     DEADRTMDLMARAGIAVVSLPMCNMYLQDRVPGRTPRRRGVTLLHEMKARGIEVMVAS
                     DNTRDPFYAYGDLDVLEVYREATRILHFDHSAPDWIRTVTRTPASFLGGGVDLGVGAP
                     ADLVLFRARHWSELLSRPQSDRTVLRRGKPIDRSLPDYRELDPVLARS"
     gene            complement(17216..18097)
                     /locus_tag="PK812_00105"
     CDS             complement(17216..18097)
                     /locus_tag="PK812_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ67985.1"
                     /translation="MSAPSLPSVPHDGTDAEARPVFAEERKILGLAASTWPGILAPLG
                     IGILALFAWEWAVNAYQIPNYVLPGPKLIGQTLVADWALLATSLQVTLTITLQALAAA
                     VFFGVTLAVLFTQSKWLEKSLLPYAVILQVTPVVSIAPLIIIWTDNNVTLSLLICAWI
                     VAFFPILSNTILGLNSADHNLINLFQMYGASRWQTLRYLKLPAALPYFLGGLRISGGL
                     ALIGAVVAEFVAGTGGSASGLAYRILESGYQLKIPRMFAALLVISMTGIAIFLIISWV
                     THLLLRHWHESAVKREN"
     gene            complement(18094..18867)
                     /locus_tag="PK812_00110"
     CDS             complement(18094..18867)
                     /locus_tag="PK812_00110"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006335604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ67986.1"
                     /translation="MATKEGATKAAPVVCSIRGVSKQFANGTLAVHDVNLDLRHGEFL
                     SLLGPSGCGKSTLLRMLAGLGSPSAGTIDWPSVDHELGFVFQDPTLMPWANALGNVML
                     PLKLKGVARADAEARSAEMLALVGLKGFEKSYPRELSGGMKMRVSIARALVTRPEILL
                     MDEPFAALDEITRHRLNDDLLKLWWERRFTAVFVTHSVFESVYLSQRIVVMAARPGRV
                     MADLAVGGPYPRDDQFRTSADYAQLCRIASDSLKKAIAL"
     gene            complement(18882..19913)
                     /locus_tag="PK812_00115"
     CDS             complement(18882..19913)
                     /locus_tag="PK812_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRJ67987.1"
                     /translation="MTMMKYRLNRRAALGLIGGSAASTLIPMPGAKVNAQTLDKVSYQ
                     TNWRAQAEHGGFYYGVASGIYKKYGIDADIRMGGPQMSPSQLLLAGRVDMIMSNSFDA
                     IRYVQENLPFLCIASIFQKDPQVLISHPNMGHDSLAALKGKPILIGAVGRTSYWPFLR
                     AKFGYADDQIRPYTFNMAPFLADKNLSQQGFLSSEPFAIQKGGVNPVVHLIADAGFEN
                     YNTTINISRKMVEEKKDVVQRFCDATFESWAAYMKKGEYFKAANDLIKKDNPEMDDEK
                     IEYAVKVMNERGIIQSGDALTLGVGAMTDARWKRFYETMAAVDVFPKGLDITKAYSLA
                     FVNKGIGKA"
     gene            20159..21526
                     /locus_tag="PK812_00120"
     CDS             20159..21526
                     /locus_tag="PK812_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012453024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRJ67988.1"
                     /translation="MNRPERIDAAPAYDIDALIAELKDIPHETERTSVRRRSRDFFWY
                     SPVLNRQLHGLAADVIFMPRDEKDVVAIAGACARRRIPVTPRGGATGNYGQAVPLKGG
                     VLLDMNGLNKVLWHKPGVVRAQAGAMLHEIDYLTRPSGWELRLHPSTKRMATIGGFVA
                     GGSGGIGSVTYGGLREPGNVMGARIVTVEETPRVLELRADAAQKINRAYGTTGIIVEL
                     EMPLAPAWTWVDLIVGCEDFETAMQVGYDVAHADGIVKKVVTLLDTPIPDYFGPIAAQ
                     CPKGKPVLICMIAEPFVEYFKALVGARGTVTYEQVSDDTPGSVPLYEYTWNHSTLQWL
                     KTDRSITYLQNLYPHDRLFESVRQMRDMFLGEVHYHLEFIRYAGKLSCSALPIVRYTT
                     EERLNEIIALHEQHGVMIANPHVYTLEEGSRHKWVDADQMNFKHNTDPLGLLNPGKMK
                     SFVPR"
     gene            21531..22115
                     /locus_tag="PK812_00125"
     CDS             21531..22115
                     /locus_tag="PK812_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003603492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRJ67989.1"
                     /translation="MRVLYLYAHPVPESFHGAIRAEALAGLAAAGHEVDLIDLYADGF
                     DPVITADQRRCYHDTTRNRAGMEDYIDRLNRAQALVVQFPTWTFGPPAILKGFFDRIM
                     IPGVAFDLSDPANVRPLLGHIRRIHGIVTYGRQRWVALVVGDGPRNTVTRYLRRLIGW
                     RARTGYDALYHMNVATDADRKHFLARVRRSMENL"
     gene            22128..22916
                     /locus_tag="PK812_00130"
     CDS             22128..22916
                     /locus_tag="PK812_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HRJ67990.1"
                     /translation="MRKLFWGDLTTEDFRSLDPEKTIAVMPIAAIEQHGPHLPVGVDT
                     EIANGMIRETIRLLPEDMAILILPTQCVGKSNEHIRSPGTLTLTAETAIRAWTEIGES
                     VQRAGIRKLVMVNSHGGNVDCIGIVARELRVRFQMLAVHCAWSRFGAPDGMFGETENA
                     VGIHAGDKETSLMLHFRPDLVKMDKAQNFAPTTIEIATSFSLLRPTGFTAFGWLAQDL
                     HPSGAAGDASRATAEKGRALAEYEARKFIELLRDVERFSLARLA"
     gene            22976..23052
                     /locus_tag="PK812_00135"
     tRNA            22976..23052
                     /locus_tag="PK812_00135"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23010..23012,aa:Arg,seq:cct)
     gene            complement(23208..23969)
                     /locus_tag="PK812_00140"
     CDS             complement(23208..23969)
                     /locus_tag="PK812_00140"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRJ67991.1"
                     /translation="MSQPLARYPDLKDQPVVITGGASGIGRAMAEAFLVQGSRVGILD
                     RDAGALQAFTSECPELAAEQVDLRDIAAMRLALARLAGRVGPPAILVNNAAHDARHDF
                     DRLEVSEWDERIAVNLRHYIFAAQAVAPAMREAGRGVILTMGSTAWMKASTNMIAYAT
                     AKSAVEGFTRSLSRELGPAGIRVNAIAPGWVMTARQIADHATPEKRAANLAAQSIRRE
                     LVPRDIAAAALFLCSSDARAITGQTLIVDGGTCYG"
     gene            complement(23973..25685)
                     /locus_tag="PK812_00145"
     CDS             complement(23973..25685)
                     /locus_tag="PK812_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017271446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HRJ67992.1"
                     /translation="MASEAEVLRPDERELPGGAAALAKGLYLLDVLGERETPARFKDL
                     QVATGLPKGTLARMLHTLVAFRLVRHEERDNTYRLGNRLFELAHRVWETFDLRGAAAP
                     QLERLALEARETVAIGTLDNDQIVYIEQRNQGGPFGFRIEVGRRAPLHCTAVGKALLA
                     FSTPHDQRAILHRIPTDRFTTTTLVDPELLLADLALTRARGYAISNGEHVDGVVSVAA
                     PIFDHTGKAIGAIGMFGPSSRNPEDRLHSFGRDLMEAARLISGNVGAPSVSIHSRSAP
                     VRGADPDVECVLPWSASLAEGPFWSIAEQKLYWVDILAPAVHRFDPAGGINESCPLPR
                     LVSSVIGRRGGGLIVTTSDGVEELDFASRQLVRRVDPEAMQPENRFNDAKCDPAGRLW
                     AGTMSLDAARKSGALYRIDPNHAWQRMDHPFTVANGLDWSPDGRVFYFTDSSAGTIYA
                     YDFDMETGTLGKRRDFVRIDPGEGRPDGLTVDSEGCVWSALWDGWCVRRFDPQGRLMR
                     ELRVPVPRPTSVAFGGPALTTLFITSARVRVPAKILAAAPFSGGILAVDVGVRGRPPS
                     LFAG"
     gene            complement(25661..26644)
                     /locus_tag="PK812_00150"
     CDS             complement(25661..26644)
                     /locus_tag="PK812_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRJ67993.1"
                     /translation="MTILVTGSAGRIGRSLVARLLARGETVRGLDLRSTVLTHDRFDE
                     VLGAFDDREAVGRAMSGVRAVFHLGAFMSWLPQDQDRLFAANVEGTRLVLEAAVGAKV
                     ERFLFASSGEVYPEGAPAFLPVDETHPTEPRSTYGLTKLLGEELVRWAGRSHGLPFTI
                     LRFSHTQDAAELLDPGSFFSGPRFFLQPRIAQQQALGNAAAVAALRAVDDGTPAHVLA
                     RNEKGRPFRMMITETRDMVEGLLASLATPRMLGETCNLGATQAVDFAEVLPKMAAITG
                     LPVRVVDLPGAGVFYETSNARFRNITGFEPSYRFDRMLEEAAVAWRAKQKS"
     gene            complement(26651..27748)
                     /gene="ugpC"
                     /locus_tag="PK812_00155"
     CDS             complement(26651..27748)
                     /gene="ugpC"
                     /locus_tag="PK812_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HRJ67994.1"
                     /translation="MAEIVIDRVRKSYGPLTVLKDFSLEVADGEFVVLVGPSGCGKST
                     MLKILAGLEEATSGVVRIGGRDVTDLAPGDRDIAMVFQNYALYPHLTVAANMGFGLKM
                     RGTPAGIIARRVQEAARILDVEHLLARRPRQLSGGQRQRVAMGRAIVREPQAFLMDEP
                     LSNLDAKLRVQMRAEIGALHQRLGVTTIYVTHDQIEAMTMANRIVIMKDGEVQQIADP
                     ETMFAQPANLFVAGFIGSPGMNFLKAETRAGRIRLFDQDVPLPPAAEGTITAGLRPEH
                     FSAGPGPVTFTTRPMLVESLGSEKYVYFEAPQANRLVNPIGRTDEERRGDRLVARLMN
                     SGPITVGEELTLSFDPARLHVFDPASGRAIR"
     gene            complement(27767..28597)
                     /locus_tag="PK812_00160"
     CDS             complement(27767..28597)
                     /locus_tag="PK812_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017276555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRJ67995.1"
                     /translation="MKQSLATRLTLYASVILTCAILIFPIYWLIVTALSQPDQLRQLP
                     PKFWPEEPRWSVFGKILEERPILLWLSNSALAAMGAVTISMVVSVLAGYSLSRFRVRG
                     GDSLGLFILTAKMLPATLLVIPLFGIFRMTGLIGSMWSIILAHATLIIPFTTWMLKGY
                     FDTIPRELEQAAMVDGCSPLGALFRVILPVSAPGLAATALYGFVLSWSDYAYARTFLT
                     NAQGNWTANLGITTMKGEYISNWADISAAAILVALPILAIYLFLERYLVGGLTAGSEK
                     "
     gene            complement(28605..29513)
                     /locus_tag="PK812_00165"
     CDS             complement(28605..29513)
                     /locus_tag="PK812_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009762371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRJ67996.1"
                     /translation="MAGGRFSLDLARPSRQHWLGYILLAPAVLLIAAIIVYPLFVSVD
                     LSFQNVGIARLDQPRRPFTTANYERLFGSPDFWNACWVTLKLILIVTTACCLLGLGTA
                     LLVNNRFKGRSLARLLVAMPWAVPEVIAVVIFAWIFDSSFGLMNWIFLSLGFIDKTIN
                     WFSDPTAAFAVVATTMIWKGYPFVSIMCLAGLQSIPEDYYAAARVDGANALQRLIYIT
                     LPNLRPVLGVTTVLVMLWIFRDFSIIYVLTGGGPLKATQTLSIMTYEQAFSFFRMGYA
                     SAVGVVTLVICVIAAKLMMRRAPDTL"
     gene            complement(29518..30792)
                     /locus_tag="PK812_00170"
     CDS             complement(29518..30792)
                     /locus_tag="PK812_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020811823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HRJ67997.1"
                     /translation="MRNRLKSLIVGGVAALAFSTLAPPALAQTKTLKFVSWQKDERGV
                     GDWWASVIKEFETKNPGVKVEWTKVERGAYADTMTTLFAGGKPPEIVHLASFEFQKFA
                     DQGWLEDLSPYIKASGLNLDGWAGQSSCQWKGKPVCVMMLYFGNIFAYNEDMLKKEGL
                     SPPTNWAEYVAAARKLTKDTNGDGIPDQFGTGHETKGGGGQYLSELMNYTLDAGGRWT
                     DANGKVTINTPQMIEGLSRWKDVVKNSLTPRDLSAGETRQMFADGRIAMKLDGPWLYP
                     IIQRGKAKDQIKIAAPPFNPPLGGSSNILGMASEIPADTKKLVWDFIAIATSDKFQTD
                     YATLAASTPPSPRAKTEGAKAATPHFDLLVQTAQAASKAGVDRIPKGLEIQYNEFAKM
                     IMEEAQRMIIQDLDPRAVAATMQAKAEAIQKQ"
     gene            complement(30822..31988)
                     /locus_tag="PK812_00175"
     CDS             complement(30822..31988)
                     /locus_tag="PK812_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020811824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HRJ67998.1"
                     /translation="MSDRIARVEAFMLTVTRETPYLGTLRPGEEVNERGYFVRRGNKT
                     VYPNFDRNVLVRITTRQGVIGWGETYGIVAPRATMEIITDLLAAFVVGRDPFDASAIH
                     DDLYNLMRVRGYTGGYYLDALAAIDIALWDIAAQIAGQPLCKLLGGQRLERIPAYVSG
                     LPEPTRQARADLAAKWQAKGFDSFKFASPVADDGVVTEIATLRERLGPSARIACDMHW
                     AQNAAQAKSMIHAMEAHGLWFAEAPVAPEDIAGLAEVAQGVSCPVAAGEEWRTAHDAA
                     ARLSRRACAILQPEMGHKGITEFMRIATLAQVHHAEIIPHATIGFGIFLAASLHASAA
                     APQVTCHEFQHSIVGQNRRYLDGEIICEAGEYHLPPGPGLGVRPSAEALRNLEQ"
     gene            complement(31985..32914)
                     /locus_tag="PK812_00180"
     CDS             complement(31985..32914)
                     /locus_tag="PK812_00180"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010974694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRJ67999.1"
                     /translation="MESTTIQGVWPVLCTPFAADGAIDRAAMRRIVGFACDSAVQGVV
                     FPGFASEVDDLTATERFELLQIVLDELGGRLPLIAGASAPTLDEVIARVREVQSLGLR
                     QVMIQAPKAIGTDAAAVADFYAAIAAACPGVEIILQNAPAPRGSDLSPAAVLAVAAAV
                     PEIRYVKEETIPSGAPISAILADAPGHLAGVMGGGGARFVIDEFLRGACGAMPAVEIA
                     DIHARMWQALKAGREAEARSLYERTLPLLMIQSHARMRLTKRVLMLRGILDNDMVRAR
                     IPPFDARDIAEIQALMDRIADLLVAAPLARVAA"
     gene            33041..33943
                     /locus_tag="PK812_00185"
     CDS             33041..33943
                     /locus_tag="PK812_00185"
                     /inference="COORDINATES: protein motif:HMM:NF016894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HRJ68000.1"
                     /translation="MKRIVVDWGSSNFRAYRFGVEGALIERHQAEAGILTVRDGAFED
                     ALEREIGHWLDPETEILLSGMITSRNGWVETPYLEPPASLSGLATRAVARPARTGARL
                     LFLPGVCNRAPQPDVMRGEEIQVFGAVSPTEDALVVLPGTHSKWVSVRAGAIAGFSTF
                     LTGEMFALLCQHSIIGRLIPPGAAGASNGAFLEGVKLGFSGNPRGLLNDVFTARAGAL
                     LGDFMPTEIAERLSGILIGHEIRAGFESAIMPGLTPLLVGEAALIARYAVAFSALGLA
                     SAKGPDHAAVEGFRKLGALGASHA"
     gene            33936..34592
                     /locus_tag="PK812_00190"
     CDS             33936..34592
                     /locus_tag="PK812_00190"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007558859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HRJ68001.1"
                     /translation="MPDHLTRFEAAFAAMPLIAILRGIRPEEAVGIGEALVAAGIRII
                     EVPLNSPEPFESITRLVRRFKAEAVIGAGTVRWPHEVERLRELGAGLVLSPHVDAAVV
                     RASVAAGIVSIPGILTPTEAFLALNSGAHALKLFPMEMIGTPGARAMRAVLPRGTRLV
                     AVGGVGEGNAAQLLDGACDGLGIGSSLYKPGDKASTVGERARRFIQATGSGSARTTSG
                     "
     gene            complement(34734..35555)
                     /locus_tag="PK812_00195"
     CDS             complement(34734..35555)
                     /locus_tag="PK812_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HRJ68002.1"
                     /translation="MARKKGGGHGGGHGWFVTFADLMGLLMSFFVMLTAFSTQDQKKL
                     QIVAGSMRDAFGSQKQSRLSGIVEIEGVPVRPHLRNLEKIPPDQASEFTAPKRAEKRQ
                     DGRTDFAHERAFALAAASLRQAFQDLPDISEMSRNVLVEETSEGLDLQIVDQDGRSMF
                     PEGSTQPLDRTRALLAALAPRLMELPNPIRITGHTSADRSGRKSADGWRLSTGRALAV
                     RDILQSSGVAAGRFESVVGKADTEPMFPDDPKLAANRRVSIVLVKSAPPLPDIRK"
     gene            complement(35571..36341)
                     /locus_tag="PK812_00200"
     CDS             complement(35571..36341)
                     /locus_tag="PK812_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRJ68003.1"
                     /translation="MDFATGLGFLAGIATVAALILMGGNFGMFYDVHAIIVIGGGCTA
                     GTLIRFPLSAMAHGFPVGIRYAFTMRSQHPRVLIEEITRIAEIVRKSGPVALEGQSVD
                     DPFLAQGLRYLADGYDPDFIRRTLEQDRDTFLQHLDEGQKIYRSIGDAAPAWGMVGTL
                     VGMVQMFSNMEDPSKLGPFMAVALLATLYGALVGNLICLPIADKLHVKLEEEEVSRTL
                     IIDGVLQMRASKNPAVIREMLISYLPDHHKHELADASA"
     gene            36539..38059
                     /gene="gpmI"
                     /locus_tag="PK812_00205"
     CDS             36539..38059
                     /gene="gpmI"
                     /locus_tag="PK812_00205"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010684929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HRJ68004.1"
                     /translation="MVPRPVMLVILDGWGERAGRQDNAVKLARTPNFDRLWATCPHAF
                     LKTSGLDVGLPEGQMGNSEVGHLNIGAGRVVMQDLPRIGEAIRDGSLARAPALTDLIV
                     ALKASGGVCHLLGLVSPGGVHSHQDHALALARILSDHGITVLLHALTDGRDTPPRSAA
                     DYVRSVETGLPARARIATVIGRYYAMDRDNRWERVSKAYEAMVSGGGQAFASAGEAIA
                     AAYADDVTDEFILPASIGGYTGMKAGDALICFNFRADRVREILASLLDPAFGGFERAR
                     QPHLAAAVGMTQYSTELDAFLATIFPPQSLANVLGQVVSDAGCKQLRMAETEKYPHVT
                     YFLNGGEEAVYPGEDRIMVPSPKVATYDLQPEMSAPELTDKAVAAIASGVYDLIVLNY
                     ANPDMVGHTGSLPAAITAVETVDAGLGRIAEAIGARGGALLVTADHGNCEQMRDLETG
                     GPHTAHTTNPVPVFLLGAHAPALREGGRLADLAPTLLALMRLDQPPEMTGRSLIMA"
     gene            38336..38425
                     /locus_tag="PK812_00210"
     tRNA            38336..38425
                     /locus_tag="PK812_00210"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38370..38372,aa:Ser,seq:cga)
     gene            complement(38674..39267)
                     /locus_tag="PK812_00215"
     CDS             complement(38674..39267)
                     /locus_tag="PK812_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HRJ68005.1"
                     /translation="MQVSAGHFKERDAVAVSLTEEAQKALLVPGSAGNGPSFAMPAIE
                     FAEGTIEVDLAARINGRGQPDVRGFVGLAFHIADDLGTFEAVYLRMTNGSRNVPPPPA
                     PRNVFAIQYISYPDRYWRKLRQEHPNRYEKAAPVAIDSWHRLRLEIDGSSLRAFVDGV
                     EVMTVADLRYPGRKGRIGLWVDDGTEAFFANLSVAPK"
     gene            39489..40403
                     /locus_tag="PK812_00220"
     CDS             39489..40403
                     /locus_tag="PK812_00220"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRJ68006.1"
                     /translation="MNKIDLRRVDLNLLPVFEVLIEERHVSRAAERLGRSQSAISHAL
                     ERLREQLNDPLLIRVGGVMTPTAYGLEIFSEIRPILRNIQRVLSTRESFVPDTSDRTF
                     HIVIPDFWAAAIAAFVRCIRIEAPNVVVMWSPPHERSLVDLANEQVDLVVAPSKLKAP
                     EGIEAESIGALSWGCFLRRGHPALETWGAEAWQAYPHIVVNVGERFPSPVQVASHPGG
                     LLRRVGAYVPTFAAVPPLLAESDMIATLPRLALVEGARVYGLQDRLPPFPIAPIDASL
                     FWSARRTSDAATRWFIERLRPALVERIV"
     gene            40505..41603
                     /locus_tag="PK812_00225"
                     /pseudo
     CDS             40505..41603
                     /locus_tag="PK812_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611442.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(41883..45080)
                     /locus_tag="PK812_00230"
     CDS             complement(41883..45080)
                     /locus_tag="PK812_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRJ68007.1"
                     /translation="MISSIFIRRPRLAVVISLIITIAGLIAYRAMPVAQFPDIVPSQV
                     SVTAVYPGADAAALEQSVAQVIEPAINGVDKMIYMQSVSGSDGVYTLNVSFAVGSNPD
                     MNTVNVSNRVNQVTALLPSEVQALGVTVTKKSTSLLQVVTVYSPKGSRDALFLSNYTK
                     LNVVDPLARIEGVGQAFAFGPRDYSMRVWLDVNRLASLNITAGEVVQALRAQNVQAAV
                     GRIGANPLVPGTNFQVNLSADGRLRTVEEFENVIVRVNPDGARVTIKDIARVELGART
                     EDSQPSFNGRPAAGLGIYQAPGANAVATAERIKTVVEGLRQRLPDDVEIKIMYDSTYF
                     VEKMMESVMHTLGEAFVLVAIVVLIFLGSFRATIIPILAVPVALVGTLAVMLAMGFSL
                     NSVSLLALVLAIGIVVDDAIVVVEAVEHKLESHPEMTVAEATEAAMKEITAPIIAITL
                     VLLSVFVPTAFMPGISGQLYMQFAVAVSASMVISAVNALTFSPAMCAVILRHKAAPKG
                     LFRMFNTGVDRMRGGFMGLIAPLARKAVLTLGLLAFFAAGTGWLAKTVPAGFLPLEDQ
                     GAFMGEVQLPAVASTERTASVGEAIRQVIAKREAIQDVFMVNGFSLLDGVILPNRAFF
                     VATMKPFDERKEASQSAFSVIASLNREFAARADAFAFAFNLPPIQGLGTGSGFQALVT
                     SLEGADPVLLAQVARSTMISANQDKRLAGVFTTFNADTPQLKVIVDREKAQTLGISVA
                     SIFSTMQTVLGGTYVNDFNLFGRTWQVNVQAEAAARMSASDIERIRISTSNGDLVPLT
                     SVVSIRLVTGPAIIMRYNNQRAAMLNGGPSPGHSSSEAIAAMEDVAKASLPRGFKLEW
                     TGTAFQEKQAGGQTTIVLILAMLFAYLCLVGLYESWTIPVAVLLSVVAGLFGAMMALK
                     LAGLDNNIFAQIGVVILIALAAKNAILIIEFAAENRAHGKGIVDSAIDAANQRFRAVM
                     MTSFAFILGLLPLVIANGAGAATQRAVGTAVFGGMLAAALVGILLIPGLYVAFQSARE
                     VVKAWMGRARAPKPEPKEPAPAQSGDAGAGHSNPS"
     gene            complement(45084..46172)
                     /locus_tag="PK812_00235"
     CDS             complement(45084..46172)
                     /locus_tag="PK812_00235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRJ68008.1"
                     /translation="MRLKLLVAMASMALLSTAQAQQSAPVPTVIVKAAELREIGRDNE
                     FIGKIEAVERVEIRARVSGLLEAPKFRDGEKVKAGQPLYLIEPDPFKAAVDAKEAQLA
                     SAKADAQNAALNLGRAQELLRTNAGTAATVDQRKAESAKADANVRIAEAALSDAKITL
                     SYTDIRAPIDGRIGRTAITEGNIVSPSNGPLAVIVKEDQMRVVFSVSQREVLEYRKRK
                     ATKEPIVRLKLADGSLYSQTGAIDYIDNVVDSRTDGQVLRATFANPGGQLTHGQTVRV
                     LIEQPAEGKQVVAPQVALAADQTGTFALVVDGQGMVSVRYVKVGQSSGDIAVITDGLK
                     EGETVIVQGMQRVRPGMKVNAQRAEAGK"
     gene            complement(46396..46814)
                     /gene="arsC"
                     /locus_tag="PK812_00240"
                     /pseudo
     CDS             complement(46396..46814)
                     /gene="arsC"
                     /locus_tag="PK812_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003614267.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance.;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
     gene            complement(46814..47872)
                     /gene="arsB"
                     /locus_tag="PK812_00245"
     CDS             complement(46814..47872)
                     /gene="arsB"
                     /locus_tag="PK812_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010398864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HRJ68009.1"
                     /translation="MSTFERYLTLWVVLCIVVGITLGHLLPGPVQAIGAMEIAQVNFP
                     VALLVWLMIIPMLVKIDFSALSGVKAHWRGIGVTVFINWAVKPFSMALLGWIFISWLF
                     RPYLPADQINSYIAGLILLAAAPCTAMVFVWSNLCKGEPHFTLSQVAVNDVIMVLAFA
                     PLVGLLLGLSAITVPWNTLVLSVVLYIVVPVIIAQVLRRQMLATGGEPALARFLAVLQ
                     PLSLVALLATLVLLFAFQGGQIIAQPLVIAMLAVPITIQVYFNSGLAYLLNRWTGEAH
                     CVAGPSALIGASNFFELAVAAAISLFGFQSGAALATVVGVLIEVPVMLSVVAIVNRSK
                     DWYEAGSAVRTRMVEGKH"
     gene            complement(47901..48137)
                     /locus_tag="PK812_00250"
     CDS             complement(47901..48137)
                     /locus_tag="PK812_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012496989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRJ68010.1"
                     /translation="MKTVKVLGPGCKRCEATVQMVRDEAARLGIDVNVEKVTDYAAIA
                     GYGIASTPGIVIDGKVVHAGGLPKEEDLAVWLNA"
     gene            complement(48150..49202)
                     /locus_tag="PK812_00255"
     CDS             complement(48150..49202)
                     /locus_tag="PK812_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006724586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HRJ68011.1"
                     /translation="MTSALRGASRHRPTWIWCLGVAAFVVIWFAVYGQLAPFSEGAVG
                     LFPLAKGSHAYEAVKFFLYDTPKVLMLLTLVVFGMGVIRSFFSPERTRALLAGRAEGV
                     GNIAAAVLGIFTPFCSCSAVPLFVGFVSAGVPLGVTFSFLIAAPMINEVALGLLFALL
                     GWKVALTYLAFGLGVAIVSGWIIGRLKLEGWLEDWVRNLRAGQADLPANRLTAVDRIK
                     AGIEAVKEIVGKVWLWIIAGIAAGAFIHGYVPSELLVSIMGRDTWWSVPAAVLIGIPM
                     YSNAAGIIPVVEALLGKGAALGTVLAFMMSVIALSVPEMVILRKVLTLKLIAVFVAVV
                     ASGILAVGFLFNFVFA"
     gene            complement(49199..49564)
                     /locus_tag="PK812_00260"
     CDS             complement(49199..49564)
                     /locus_tag="PK812_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRJ68012.1"
                     /translation="MLLTAYAFHCISPCMPQLDTLLSALSHPARRSALRLLAGGDEHC
                     LCELMDRLGVSQSSMSRHMACLKEVGLISDRRDAQWMRYRRRTLDDPILQAVVDAVLA
                     ASDDPPLSEGTSSCQRSAA"
     gene            49821..50588
                     /locus_tag="PK812_00265"
     CDS             49821..50588
                     /locus_tag="PK812_00265"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRJ68013.1"
                     /translation="MSQFEMQIRAIARQYEILSKGDGQKANVVAEAILRCVENGYWQP
                     NDKMPAELSLAKALPVSLGTIQAAYRMLTAEGVVARRRKDGTRLTSQVDVNSLNWHLR
                     FSDMETGASLTCRSTVFSITKTHDSGGWSKFLPDVAEFTCIARRWSIGERFQVLSRLY
                     ISAEFYEPLAELGMNALNDISLRAVFSDTLRIPTLRATHSISFRPPTEEEVAVLAVMT
                     DRPLMVLEACGYTYRDQPFSYQVFSMPTELRNVRLMV"
     gene            complement(50612..52876)
                     /locus_tag="PK812_00270"
     CDS             complement(50612..52876)
                     /locus_tag="PK812_00270"
                     /inference="COORDINATES: protein motif:HMM:NF023649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HRJ68014.1"
                     /translation="MISDDLMGDVWPVVQPGRATSDYARVTNASSAPCEVRSWQEISL
                     TLIVGRFGFDDTGGFRVSLRFTYDGGALQSHDPGAANYISAKSSTGVRIAVHCENHGL
                     RPWDKTVRFEVVGGALKPGDEIYVTFGDRSQGSPGFRMQTFCESAFELRVSVDPCATG
                     FFVPLEDRVTIPVLPGPPVRWKVINPTARRVGATIAVGIVGEDRWGNASDAVKGPLVL
                     QSTGPASIPAVRIDWRPGARSHRIEQRIDVPGTYRFSLFDAAGAKLAEGVPLSIGDTD
                     QTFWADLHGQSGETVGINSIEEYFTFARDIAFVDACAHQANDFQIKDAFWDEINTTTR
                     AFNDDGHFVTFPGYEWSANTAVGGDHNVFFRDEDRPIIRSSHAMIENRHRLQTDANDL
                     PALYAALAKEDCVLFAHVGGRPANLAAAHDPRLRVAVELHSDWGTFEWMLHDSFRLGY
                     RVGVVCNSDGHKGRPGASYPGAASFGALGGLTCFRAAALDRDGLFNAIRRRNHYGTTG
                     SRLHLDLHLAFDAPAEIFHTDPATGAGPHGASSRAEMGEIARTSAEAATVNISVSAGS
                     PILSIDLFTASDIAATFRSYTRDDLGNRLRILCNGAEYRGRGRQTKWTGTVTFSDAAV
                     AQITPINWWNPERPLVHDGPGRIRFETVTTGNFVGFDAALDRLQGAANIATNVVTGSF
                     DLKQLGVEACVLDAGGLDRAISVQRLPDRLDATEMTVSHRVWLKHAGDTPVWVRVRTE
                     DGHVAWSSPIYVIR"
     gene            52985..53740
                     /locus_tag="PK812_00275"
     CDS             52985..53740
                     /locus_tag="PK812_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012654959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRJ68015.1"
                     /translation="MQALVATDIRKAFGSLEVLRGVSLRANKGDVIAIIGGSGSGKST
                     FLRCLNLLETPDAGSLWLNGLALDFSRSAPSNGVAPKAKAELRRRLGMVFQSFNLWTH
                     MSALDNIIAGPIHVLGHEKKEARAAGLELLDRVGLADKAGAFPAQLSGGQQQRVAIAR
                     ALAMRPEALLFDEPTSALDPELVGEVLKVIQGLAAEGRTMILVTHEMAFARDVATHVM
                     FFHDGRVEEEGKPSLIFSDPGSERCRSFLKKFL"
     gene            53754..54584
                     /locus_tag="PK812_00280"
     CDS             53754..54584
                     /locus_tag="PK812_00280"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRJ68016.1"
                     /translation="MKTGLKHLVLAIPLCFMGTAHAQTPVVKIATEGAYAPYNFKDAS
                     GKLVGFEIDLAAELCKRATLTCEVHEQAWDGILPSLMANKYDAVMAAMNITDKREAVI
                     SFSRPYVKTPIRFVTTKKGTGADYVSSVTAITLEDVDAAEVAEIGKLAKLLAGKTIGV
                     QAATAHEAFVKEYMKGATIKSYDKMDNMILDLTAGRIDVGYTSVTFLAPILKKTPDLV
                     EFGPPMTGGALGRGVAVGVRKADTELQKKFTTAIEAMLADGSLSALAVKWFGLDISAK
                     "
     gene            54613..55326
                     /locus_tag="PK812_00285"
     CDS             54613..55326
                     /locus_tag="PK812_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012654957.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRJ68017.1"
                     /translation="MDLGILTWGDTGWGDELARGVLTTVAIATAAYLVGTLLGILLAT
                     MKLSRSPALNVPATIYTTTMRGMPEMLVIYLVFFGGGALSTWIASSVLGIRGVFDLPS
                     FAVGVVCIALSVAAYACEVIRGAVLAVPGGQIEAAEAVGMSRRQRNRLIIAPQAFRIA
                     LPGMTNVWQFALKDTALVSIIGVTEVMRTASIGSDATKQPFTFYFVALMIFITIAYLS
                     QRLFVRLQHSADRGVRWGA"
     gene            55314..56018
                     /locus_tag="PK812_00290"
     CDS             55314..56018
                     /locus_tag="PK812_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012654956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ68018.1"
                     /translation="MGGLDFLTFFGDALSQLSAGIPRTMALAGTSLVVGFVLAIGVAG
                     GLLSREVVPRSIANGYVYIFRSSPLLVQIFLIYYGSGQFRGFFDAIGLWGLFREAWFC
                     AILALALNTCAYQAVLIAGGLRAVPWGQIEAAQAVGMSRYRVFVTVAFPQALRHALPA
                     YSNEVVLMIKATALASTITILEITGLAKQMASETYRPFEVFVAAGLIYISINGAIVTG
                     FMQLERYLRQNATLRT"
     gene            complement(56073..57119)
                     /locus_tag="PK812_00295"
     CDS             complement(56073..57119)
                     /locus_tag="PK812_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008877918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HRJ68019.1"
                     /translation="MDNRKLNIVRFVSVDGMMKLILKRGIEQVLTELAARIEADFRRW
                     EHFDKTPRLASHSTDGVIELMPTSDGGQYAFKYVNGHPKNTRDGRQTVTAFGVLADVD
                     TGYPLLLTEMTILTALRTAATSAVAARHLAPPGARTMALIGNGAQSEFQALAFKALLG
                     IDRLRLFDIDPAASSRCARNLARFGFTIEICRSAEAAVMGAEIITTVTADRANATILT
                     DNMVGAGVHINAVGGDCPGKTELHADILKRSDIFVEYPPQTRIEGEIQQLAADHPVTE
                     LWRVIAGEERGRRDPSQITLFDSVGFAIEDFSALCWLAGEIGRTGLFDELDLLADPDD
                     PRDLFGMLLRAEVA"
     gene            complement(57121..58050)
                     /gene="rocF"
                     /locus_tag="PK812_00300"
     CDS             complement(57121..58050)
                     /gene="rocF"
                     /locus_tag="PK812_00300"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019904730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HRJ68020.1"
                     /translation="MTDKTCILIGAPVQDGTGRLGCDMGPSAFRAAGIGPALESLGYA
                     VRDRGNLMPQIRPGAPHPNAAIRSLPEIASWTAMLADAVLTAHREGLPIVLGGDHSLA
                     AGTLSGHARHAAESGRKLFVLWLDAHPDLHTLDTTTSGNLHGVPMAYALGLPGFAGHF
                     PPLETPLDPARICMMGLRSVDPAERLLLAQTGIRVHDMRAIDENGVATLLRDFLEEVV
                     REGGCLHVSLDVDFLDPEIAPGVGTTVPGGATFREAHLIMEMLHDCGRVTSLDLVELN
                     PFLDERGRTARLMVDLAASLMGRSVLDRPTRSF"
     gene            58171..58617
                     /locus_tag="PK812_00305"
     CDS             58171..58617
                     /locus_tag="PK812_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRJ68021.1"
                     /translation="MDDLDQRLITLLRHDGRRSVSDLALALKVSRATVRARMQRLAQS
                     GEVIGYTVVLKADVVSAPVRGITLIEVEGRTKDRVVNALSGFAEITAIHTTSGKWDLV
                     AELSTDSLTALDGLLHRIRLIAGIGASETHLLLATPRSSQARLSSG"
     gene            complement(59107..60117)
                     /locus_tag="PK812_00310"
     CDS             complement(59107..60117)
                     /locus_tag="PK812_00310"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRJ68022.1"
                     /translation="MTKRTAQSSSIKDVAALAGVSIATVSRVVNGVANKASAETVERV
                     RKAIATLEYRTTSAGRDLRQRTSRLVAVLAANLSNPAMAAIAASAETALRRSGLVMVL
                     CDTHDQPDLQDEYLREMLAQQARAIVLLGAVESPTLRRLQGSTTPVVFVNRRNPTGAA
                     GSFIGIDNRQAGEDVARWAIKLGLQNTALIHAPKTSSATRDRVAGILFGFAAAGAPIQ
                     DHLVLSPNAGDHLNIGLQAAQQLLAMGLAPDLVIGTSDLIAFGAARAWREAGRPAVPR
                     MIGFDDSPMNEWLAPELSSVRIPYDAFGAAIVQALSMQNTPAQIVLHHEIIERPKPPS
                     DQ"
     gene            60234..61514
                     /locus_tag="PK812_00315"
     CDS             60234..61514
                     /locus_tag="PK812_00315"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRJ68023.1"
                     /translation="MSILRTLACAAFAAVVSALPAKAQQLTVWHDLGDNGIKWFEALS
                     AEFAKTKPGVTVRSQSFPTDQWFGRVISALNTDTAPDLIFNNYERVIRIQNQTSKVMD
                     LKAMTAAIGDKAFLSEDDMRVATFKGRQIIIPIQRVQMALGVRKSWLDKNGGKFPNTW
                     EEVKTLAAAFRDKDPDGNGQADTFGLALQAAKPRDLIHMLDLFIFGSGLRHTLIDPEG
                     KITIDQPAHAQVLEEFLKTYSSYKFVSPDTINHSFAEMYQIIEGGRGGMFRVGDWNVR
                     KWDGPQVLNGDFVSGPWPKFAADRPNAVVIGGMRGVAIPENSPNKALAQEFAAFMLTK
                     AAQQASLENIGAGVRKDLDVSKLSERSQYFAKAEHRLVAYDFPESIHTFYPELEAAFH
                     RKLLSGIANPPADWKAFIAETAKEMRDLAAKLKG"
     gene            61579..62469
                     /locus_tag="PK812_00320"
     CDS             61579..62469
                     /locus_tag="PK812_00320"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRJ68024.1"
                     /translation="MMDALGGLSRDRVFYLFVAPFAALTILFGVWPILLSIQVSFTAS
                     ATALRPNPTYVGLANYIAVLADPIFLASLSRTLLYTVLSVIANLAFALGFAILFDSSL
                     LKHGNLFFRLAIFLPVVTPDVAGYVVWRWLYDRSFGAINAALKMLGLPAFGGLSSVDT
                     AMLSILIAELWHHAGFYMIIFLANLSIRDRALEEAAHIDGATTWQRWWHVILPQLRPA
                     LVINIVYALIQFLKTFTVVVVMTKGGPNDQTNFVSYYAYQLFDQARYGEATAMATILF
                     AIVIGLSLTAYRIGDREPGK"
     gene            62466..63311
                     /locus_tag="PK812_00325"
     CDS             62466..63311
                     /locus_tag="PK812_00325"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRJ68025.1"
                     /translation="MSDDIRPNRLLQIGAYAVAILTSVLFLYPYWWMLVSAFRPTRAI
                     MSAPLRPWPETLSFQIFSEIGRIGGVNLSTYAVNSLAITTLSTILGIIVTALGAYALV
                     RRPKAPGFALVRYGFLITIMYPYMLLVIPVYLVMFKLGLLGSYAGIILFLALGPLQFF
                     LFEQFFRKIPNEVIEAAVIDGASEWQILTRVVLPMAMPITATVALITFLLNWAQWFPI
                     MVISRSPDTYTLPVALLSMNSELGANFQGIMALAVLITLPVAALFLLAQKRVMEGMAA
                     GAVKG"
     gene            63316..64482
                     /locus_tag="PK812_00330"
     CDS             63316..64482
                     /locus_tag="PK812_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68026.1"
                     /translation="MLKTIDSREKLAIGIDNARAATLDWLKAMQAAGKPKGCMRISAV
                     HDADRWPGILLPGTYNGIMCLDLLGGLADWSADERAALGAWLEASRLPDGRFRVEGMH
                     EADVFKKPSIDETWRYIDFHVTNYTLGAIEALAPDRRPILEFAKDYLDEKTLGHWLSL
                     RDLRDPWQEGNNIVNLASFLLLLRQHGSGHQGGSTWQAEVDRALEQLFAWHDRLREPT
                     TGFWGVGQLSDTTQLLHAFAGSMHNFHIWYHENRALEGQDRAVDYCLSLPQRIDSACI
                     DVDAVDVLVHGWRLTDHRRNDIEAWLAALLPKLLAFQNPDGGFCDVRSGVRRQDGWVN
                     GYEEPQGHSNTFATWFRWIAIAMIADVLWPGRWNWKFRRMIGIGYRTANWTAAA"
     gene            64479..66263
                     /locus_tag="PK812_00335"
     CDS             64479..66263
                     /locus_tag="PK812_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006940424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HRJ68027.1"
                     /translation="MTAHPADDPFAHLHDEDYARPFEQRMATSKTLVTLAGRKTISLD
                     GQWRMTLDLFDEGLRQRWFALDEMPPSQWATPRDYEIEAGELVPVPSCWNVLKPEWTY
                     FEGAAWYTRWFDWQPGEADERTILSFGAANYAALVFLNGAHVGGHRGGSTPFCIEVSA
                     HLNPGRNRLQVYVENRRRPDRVPMHHIDWFNYGGLYREVAIARLPQVFIRRASAILLQ
                     DRKSIRFDIALSDPIEGEARVSIAELALDVTLPVKGGVGSVVIPASPELWSTESPRLY
                     DVRFAFANDVVTERIGFRTIATRGTDILLNGSVIWLKGVCVHEDDLERGKVSSEADIR
                     RRFRHAKELGCNFLRLAHYPHHDLVACIADEEGLLLWSEIPVYWAIDFANPDTLADAR
                     NQLSELILRDINRASVIIWGVGNENADTDARLAFMGELAATAKQLDPSRLTSAACLIN
                     REHFMIEDRLAGHLDVIGLNEYFGWYEPDFSGLDRLLANSNPDRPVIISETGADAAPG
                     HRGAGRMLFSEDWQAEFYRQQFQRIAATPFVAGIAAWLLYDFRTERRQTGFQQGFNRK
                     GLIETDKSTKKLAFDALADCYRRKGPSS"
     gene            complement(66622..66706)
                     /locus_tag="PK812_00340"
     tRNA            complement(66622..66706)
                     /locus_tag="PK812_00340"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(66670..66672),aa:Leu,seq:caa)
     gene            66863..67444
                     /locus_tag="PK812_00345"
     CDS             66863..67444
                     /locus_tag="PK812_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018044975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HRJ68028.1"
                     /translation="MLRRLYDWTISLAESPRATVALGAVSFAESSFFPVPPDVMLVPM
                     SLAKPKKALFYAMLCTITSVAGGILGYAIGALLYDTLGKWIVELYGMTDKLEHFRDMY
                     RQYGAWIILIKGLTPIPYKLVTIASGLAGYDFLMFVVLSLITRGARFYLLAGILNYFG
                     EPIRKFIEANLTLMATLVIAAVLGGFYVVKFLF"
     gene            67454..67960
                     /locus_tag="PK812_00350"
     CDS             67454..67960
                     /locus_tag="PK812_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007558875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HRJ68029.1"
                     /translation="MRVVTVRADVLWLGLAGALATAALLGAWIFELGLGYLPCKLCLW
                     QRWPYYIGLPLALLGLFMAWSGADRRVVLVLGSLFALVMLAGAALGVYHAGAEWKFWP
                     GPTDCGGRIGAMPGSVDDFRKSLTTARVVRCDEAALRVLGLSFPGWNVLVSVACVAAT
                     MLGLRRIP"
     gene            complement(67968..68537)
                     /locus_tag="PK812_00355"
     CDS             complement(67968..68537)
                     /locus_tag="PK812_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HRJ68030.1"
                     /translation="MTVHVPPAVSPDACPALVLNADYRPLSYYPLSLWSWQDALKAVF
                     LDRVHIVSEYDRVVRSPSFEVRLPSVVCLKTYVKPSRAPAFTRFNVFLRDRFSCQYCG
                     SREELTFDHVIPRSKGGLTVWENVVAACSPCNLKKGDRLPMDIEMWPRQAPFQPSLQH
                     LHANGRLFPPNYLHQSWMDYLYWDSELEP"
     gene            complement(68692..68964)
                     /locus_tag="PK812_00360"
     CDS             complement(68692..68964)
                     /locus_tag="PK812_00360"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRJ68031.1"
                     /translation="MQDARQQARLKTYIGARIEQPYPLGTMNCIVRDLSTGGARIAFH
                     NTASVVNRFTLVMPCKGRRVAARVVWRNFGEAGVAFEEGGGRAGHA"
     gene            complement(69062..69943)
                     /locus_tag="PK812_00365"
     CDS             complement(69062..69943)
                     /locus_tag="PK812_00365"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013650881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HRJ68032.1"
                     /translation="MTFEIRKIGIVGAGQMGSGIAHVAALAGYGVALHDVSEDRINSG
                     LATINGNLSRQVQKSVVSDADKKAALDRIAPAATLADLSSCDLVIEAASENESVKRRI
                     FQDLSRTMKPEAIMASNTSSISITRLASTTERPERFIGIHFMNPVPVMQLVELIRGIA
                     TDDTTFRAAKAFVTTLGKTITVSEDFPAFIVNRILIPMINEAVYTLYEGVGSVEAIDT
                     AMKLGANHRMGPLELADFIGLDTCLAIMQVLHDGLSDSKYRPCPLLVKYVEAGWLGKK
                     TKRGFYDYRGEKPVPTR"
     gene            complement(70021..70965)
                     /locus_tag="PK812_00370"
     CDS             complement(70021..70965)
                     /locus_tag="PK812_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRJ68033.1"
                     /translation="MATLVIAEHDNVSIKDATAKTLRAAAAMGAPVHVLVAGKNCGGA
                     AKAAAGLAGVEKVLVADADAYANMLAEPAAALVAGLAGGYGAIVAPATANGRNIAPRV
                     AALLDVMLISAVTKVISADTFERPIYAGNAIQTVKSNDAKKVVTVQTASFQAAAGGGS
                     AAVEAVAAAADPGVSTFKGDALSKSDRPELASAKIIVSGGRALASSENFKTYIEPVAD
                     RLGAAVGASRAAVDAGYAPNDWQVGQTGKVVAPELYVAVGISGAIQHLAGMKDSKVIV
                     AINKDADAPIFQVADYGLVGDLFQVMPALEAELAKIKG"
     gene            complement(70968..71717)
                     /locus_tag="PK812_00375"
     CDS             complement(70968..71717)
                     /locus_tag="PK812_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006936816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HRJ68034.1"
                     /translation="MKILVPVKRVVDYNVKIRVKPDGSGVDLANVKMSMNPFDEIAVE
                     EALRMKEAGKASEVVLVSVGPAQAQETIRTGLAMGADRGILVKTDQAVEPLAVAKLLK
                     AVVGTEAPGLVICGKQAIDDDTNATGQMLAALLGWSQGTFASKATIGDGSVDVTREVD
                     GGLQTVSLKLPAVLTTDLRLNEPRYASLPNIMKAKKKPLDETTPEALGVDIAPRLSVL
                     KTSEPATRKAGVKVADVAELVGKLKNEAGVF"
     gene            complement(71835..72542)
                     /locus_tag="PK812_00380"
     CDS             complement(71835..72542)
                     /locus_tag="PK812_00380"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRJ68035.1"
                     /translation="MFLPINDGEPLRRLASPWVTWGIIGVTCAIFVLFQSDFLVRLEP
                     QVSLGFGLIPSVVFGTDVLDPRIVHAPAWLTPVTHLFLHGNLVHLAGNMLFLWVFADN
                     VEDEMGHARFAVFYLLTGIIAGLVYALTASTSQAPLVGASGAVSGVLGGYLLLHPNVR
                     VFGLFLNIIPVRIPAFWFILGWFALQVGHAVFDPNRSVAWIAHVVGLIAGAVLVIPFR
                     KDMLPARPVLRRRTHIT"
     gene            complement(72607..>72793)
                     /locus_tag="PK812_00385"
     CDS             complement(72607..>72793)
                     /locus_tag="PK812_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010970094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HRJ68036.1"
                     /translation="ARTVARRAERAMVTLARQPGETVGTPALHYINRLSDFLFVAARI
                     VNDEGRADVLWTPGQNR"
BASE COUNT        13523 a        23012 c        23105 g        13153 t
ORIGIN      
        1 cggcgagggg ctgccgcgca cgatcctcga agcggcggcc tgcggacgcg gcatcctgac
       61 gacggatgtc ccgggttgcg ccgacttcgt gcgtgatggt gtcgaggggc gcgtgctggc
      121 gaacggcgag gccgatccgt ttgccacggc actctgtgac ctggcagctg atcctcaggc
      181 ggttatccga atgggagaga gcgcgcgcag ccgggttttg gccggctaca ccgaggcggc
      241 tgtcggccag gccgtggcgg cactctatcg agacatgctc gcatgagcgc ggccggtccg
      301 gttcgccgcg agacgttcat ccgcgccgag acgaaagtcc tgccggtgcc gcatgcgccc
      361 gagattcggc tgcatctggc ggacgaggcg atcccgctgt gggagaaaac cgaagaacag
      421 ctcgcggcga tcggccttgc gccgcccttc tgggcttttg cctgggcagg cgggcaggcg
      481 ctcgcccgct actgtctgga tcatcccgat ctggtgcgaa ggcgccgcgt cctcgatttc
      541 gccgcaggct cagggcttgt cggcatcgcg gcggcgaagg caggagccgg cgcggtcgaa
      601 gcggtcgaga tcgacccttt cgccgtgacg gcgatcaacc tgaacgcgcg cgagaacgac
      661 gtgaccttgt ctgtgcggca cgccgatctg atcggccagg agggaggctg ggacgtgatc
      721 ttcgccggcg acgtcttcta tgaacgcgac atggcggaaa gggtgttccg ctggcttgat
      781 gcactgcacc ggcgcggcgc ggtcgtcctg atcggcgacc cgggccgcag ctacctgccg
      841 aaggatcggc tggaagcgct ggcgacctac caaatcccgg tgacgcgcgc gctggaggat
      901 gccgaagtca agcgcacttc ggtctggcgc attcgcggct gaccggctcg cgccgggccg
      961 caaaccgcgc tatatccgcg ccatgcaaga gcatgcctgc ggccaccatc acgaccaaga
     1021 cagcggattg cccgttggag aacgcctccg gcaggccgaa atcctgtgca ctggccgcgg
     1081 cacgcgtctg accgcccagc gccgcgtggt gctggaagcg cttctgacgg cccgccgggc
     1141 gctcggcgcc tatgacctga tcgagcaggt cagccgggca accggcaagc ggacagcccc
     1201 gatcaccatc taccgcgcgc tcgaattcct gcaggagatg ggtctcgcgc atcgcattga
     1261 gtcgcgtaac gccttcctcg cctgccacgg gggccaccgg ccacatgcac tgacggtctt
     1321 catgatctgc gactgctgcg gtcaggtcgc ggaagcggac gctcccggcc tggaggccga
     1381 actggatcgg ctcgccaccg gtcacggatt ccgggcacag agccgcgtca tcgaaatggc
     1441 ggggcgctgc agcacctgca acgaggggcc atgatactgc aaggcaagat cgtagcggag
     1501 ccggcctacg gcccgttgga acggcatcgc acggtggggg tgcgcatcgg cgacggcgct
     1561 ggcgccgtct cggttggggg cggggcgccg atcatcgtgc aatccatgac caacaccgac
     1621 acggcggaca tcgaggccac ggttcggcag gtggcggcgc tggcccgcgc cggatcggaa
     1681 gtggtccgca tcacggtcga ccgggacgag gccgcggcgg cagtgccgca catcaaggag
     1741 cggctgctcc agcgcgggat caccgcgccg ctcgtgggcg acttccacta tatcggccac
     1801 aagctgctgg cggaccatcc ggcctgtgcc gaagcgctcg acaagtaccg catcaacccg
     1861 ggcaatgtcg gtttccggga aaagaaggac cgccagttcg gagccatcgt cgagcaggcg
     1921 atccgccatg gcaaggccgt gcgcatcggc gccaactggg gctccctgga ccaggagctg
     1981 ctgacgcatc tgatggaccg gaacgccgcg tcggcgcagc cgcgcgatgc gcgggccatc
     2041 acgcgcgagg caatggtgca gtccgcgctt ctctccgccg cgcgggccga agagatcggg
     2101 ctggcgaagg accggatcat cctgtccgcg aaggtctcgg cggtgcagga cctgatcacg
     2161 gtctaccgca tgctggccca gcgctccgac tacgcgctgc atctcggcct gaccgaagcc
     2221 ggcatgggtt cgaagggcct tgtcgcctca tccgccgcga tgggcatcct gctgcaggaa
     2281 ggcatcggcg acaccatccg ctattccctg accccggaac ccggtggcga ccgcacggcg
     2341 gaggtgagag cggcgcagga actgttgcag accatgggct tccgcacctt cgtaccgctg
     2401 gtcgccgcct gccccggctg cgggcgcacc acctcgactg tgttccagga gctggcgcgc
     2461 gacatccagg actggatctc cacgtccatg ccggagtgga agacacgcta tcccggcgtg
     2521 gaaaacctca acgtcgcggt gatgggctgc atcgtcaacg ggccgggcga atcgaaacac
     2581 gccgacatcg gcatttcgct tccgggcacc ggagagacgc catccgcccc cgtcttcatc
     2641 gacgggaaga aggccgcgac gcttaaaggg gccggcatcg ccgccgagtt caaggcgatg
     2701 gtggggactt atatcgagac gcggttcggc aagtaggttc aacccgttcg gcatttcgcg
     2761 cacacgcggc cggacaagaa cctacgccgc aagccccttg aacatcggca gcccgtcggt
     2821 tccgcccatc atggcttcga ccaggttttc cggatgcggc atcatgccga gcacgttgag
     2881 cttttcggaa tagattccgg cgatgtggtt gagcgaaccg ttgacgttgg ccgcctcgtt
     2941 gatgtcgccg gcggcgtcgc aatagcggaa ggcgacgcgg tcctcgccct ccagccgctt
     3001 cagcgtctcg gcatcggcgg tgtaattgcc ctcgccatgg gcgatggcga cctcgatcac
     3061 ctgatcgcgc cggtagagtt tcgtaaacgg cgtgtcggtg cgcccgacct tcagatgctg
     3121 catgtggcag aggaacttca gcccggcgtt gcgggtgaga atgccgggca gcagaccgga
     3181 ttcacacagg atctggaagc cgttgcagat gccgagcacc agcccgccgc gcgcggcatg
     3241 ggcgcgcacc gcatccatga tcggcgcacg cgcggcaatc gcgccgcacc tgagataatc
     3301 gccataggag aagccgccgg gcagcacggc gagatcggtt cccttcggaa gttcggaatc
     3361 ggcgtgccag gccatcgaga ccttcgcgcc gccgaggcgc agggcgcggg cgacgtcgcc
     3421 ctcccggttc gatccgggga agagaatgac ggcggcgtgc atcacagcac ctcgacgcgg
     3481 aaattctcca caaccgtatt cgccagaagt ttttcgccgg cctgtttcag gatcgcttcg
     3541 gcgcgggact tgtcctgcgt ctcaacctcg atgtcgaaga ccttgccctg acgcacagac
     3601 gtaatgccct cgacgccgag cgacttcagc gcgccttcga tggccttgcc ctgcggatcg
     3661 aggacgccgg tcttgagggt gacggtgacg cgggctttca tggcggcatt ccttgggagt
     3721 cgggaagaca gcctttagag gaaaggcttg ccccgctcaa tccatgcgcg gcgcgttcca
     3781 gccgcgatca accgtcgcgc catggaccgg cggagccatg cggcccgcct ccgcaggcac
     3841 tgcggcgttt cgccgtcagt caccctggcc cgggttcggg gagaagtact tctccagctt
     3901 gcccttttct ccatcgaact gcttggcgtc ggccggcgac tcgcgcttga ccgtgatgtt
     3961 tggccagctc ttggaatagt ccgtgttgag cttcagccag ctctccaggc ccggttcggt
     4021 atccggcttg atggcttcgg ccgggcattc cggctcgcag accccgcagt cgatgcattc
     4081 gtccgggtgg atcaccagca tgttctcgcc ctcgtagaag caatccaccg ggcagacttc
     4141 cacgcagtcc atgtacttgc acttgatgca attgtcggtg acgatgtagg tcatcgcgcg
     4201 caaacgctcc tgagcgaagg ttcaagcttc aatcttcggc cgcttgggta gccgagcgct
     4261 tcgccccccg caaggggcga tttggcgcgg atcgtcagga tttcggcggc ccggagcgac
     4321 cgaagtccgg cgcggccgtt tcctgccccg aggcgatgat cgactttcgt atggcgcgtg
     4381 tgcgcgtgaa caacgcgtgc agaccatcgc cgtcacccca gcgcaccatg cgggcgagct
     4441 gcgccagatc ttcgctgaag cgggcaacca tttccagaac ggcctcccgg ttgtgcagga
     4501 agacatcgcg ccacatcgtc ggatcggagg cggcgatgcg ggtgaaatcg cgaaagccgc
     4561 cggccgagaa cttgatgacc tcgccttccg tcaccgtctc gaggtcatcc gccgtgcgca
     4621 cgatgttgta ggcgatgaga tgcggcacat ggctggtgat ggcgagcacc agatcgtgat
     4681 gttgcggtgt catcacctcg acattcgagc cgatggcttc ccagaaagcg cgaatcttgg
     4741 cgaccgccac ctcgtcctgt ccttccggcg gcgtcaggat gcaccatcgg ttggtgaaga
     4801 ggctcgcgaa cccggcgtcc gggccggaga actcggtgcc ggccacggga tgggccggca
     4861 ccagatgcac gttcgccggc aggtgtggcg cgacatcgcg caccaccgct gccttgaccg
     4921 agccgacatc ggacacgatg gcgcccggct tcagacaagg gccgatggcc gcccccaccg
     4981 cagcattggc accgaccgga acgcacagga tcacgccatc ggcgccgctg acgccggcct
     5041 gcggagaatc cgtgatctcg tccgcgagcc cgagttcgcg gacacgcgct cgcacgaact
     5101 cgctggcatc gaccgcgaca atcgtcccgg ccagatcctt ctccctgacc gcccgcgcga
     5161 tggatgagcc gatgagtcca aggccaatca ggcacagacg ggagaaccgg gcactcatga
     5221 cgccatgaag tccctgagcg cctggaggac gaggcggttg gattcgttcg aaccgacggt
     5281 cagccgcagc gcattgggca ggccgtaggc gccgacgccg cgcaggatca ggccgcgctg
     5341 ggtgaggaag gcgtccgcgt ccttcgccgt cttgcctgcc ttcgatgcaa agtggatgag
     5401 caggaagttg ccgacgctcg gcgtcaccgt caggccaagt gcggtgatct cgtcggtcag
     5461 cgccttcagc cagcgctcgt tgtgatcggc ggtcgccgcc acatgcgcat cgtcgccaat
     5521 ggccgcgacg ccggcggcga tcgccggaat cgacaggttg aatggcccgc ggatacggtt
     5581 gatcgcatcc gcgacatgcg ccggcatgta cccccagccg atgcgcaggc ccgcaagccc
     5641 gtagatcttg gagaaggtgc ggcacatgac cacgttctcg ctggtcgaca ccagttcgat
     5701 gccggcggaa taatcgttgc ggcggacata ctcggcatac gcggcatcca gcacgagcag
     5761 gacgtccggc ggcaggccgg catgcagccg cttcacctcc gcgaacggca ggtaggtgcc
     5821 ggtcggattg ttggggttgg cgaggaagac gatgcgcgtc ctcgtcgtca cggccgcgag
     5881 gatcgcgtcg atgtcggcgg tgaagtcctt ctccttcacg accaccggct tgcccccggc
     5941 ggcagtgatg gcgatgcggt agaccaggaa tccatgctcg gtgaacacgc cctcgtcgcc
     6001 cggcccgaca taggcatggg tcaaaaggtt caggatctcg tcggacccag ctccgcagac
     6061 gatccgggcc ggatcgaggc cgtagcgcgc cgcgatcgcc tggcgcagcg ccgtggcgga
     6121 gccgtccgga tagtattcca acccttcgga acaggcgcgg taggcctcga tcgcccgcgc
     6181 gctcggcccc agcggatttt cgttggagga gagcttgtgc agcttcacgc cggccggcgc
     6241 ggcgctcttg cccggaacat aggcttcgat gtcgagcacg cccttgcggg gaaggggacg
     6301 cgtggaggca ggaacggaca tggggaccct ctgggcaatc gatgtcagga ttcgccgtgg
     6361 acgtaaggcg cggcgtggct gccgacctcg gcatagcggg ccacgacgcc cgcagatttc
     6421 agggcgtcca ggacagcgcc cggcgccacc gcgccaccat ggctcacgag taccgccagg
     6481 ccgctctcga cgccggcaga ggcttcgagg cgcacgccga cggagcgcag cgcttcggga
     6541 acctcgcgcc gccagcgctc cagccagagg ctggcgacga tctcgtcgcg cacggcggcc
     6601 tccttcaacg gcttggccac cacgaacacc ggcatgccgg aggggtggtt ggcgcgctgg
     6661 atgaagggca ggcgggcgat gatcttcggg cggttgtcgc catgcagcag gcgccaccag
     6721 gcgcccgcct cgatgctctg gtcgcgcggg aacacgccga ggtccccgga ggcgctatcg
     6781 accgcctcga tggctgccgc ggcgctcggc gtcggcagga agggcacggt gaagccgaaa
     6841 tggaaacgca cgctatcgcg catcgccgca tcgccgccgg acacgtcgcc atgcaccgag
     6901 aatggcacct gcgtgtaggt cgacgtggcg atgatgacgc gccagatgtt ttccgtgccg
     6961 tccagcggat aggacccgcg atgccgttcg gcgagcatgc gcatgatctg cgcctcacgt
     7021 cccggacgga acgcggatcc cgcggtgccg gccctgcgct tcacctgttc gacctgatcg
     7081 gtgacttcgc tgcgctcgat cagcagcgcc tgcatcgcgg catcgatgcg atccagctcg
     7141 gcgcggagtt ttccgagatc ttcggtcggc ttgggcggca tgacgcgggc acgctcttac
     7201 aatgcgggac cgctggtcct tagcgccttg caggacgcga gggaagagca ccgcatcagg
     7261 cacggcgcct ggccggcatc cggtgacggc tatcgtttct tgtggcgtcg gccaggccca
     7321 tcgcgaaggc gggggatagg accagtgaaa gcgcagcgtt ccctagcacc gcagagccaa
     7381 acatgcctct ggaaaccatg agcatcgttc gctacaaaga acgaaacgag cgcccttccg
     7441 aagttgttcg ttcaatgagc acgaaatggc ccgcaagccc acgcctgccg cttcccgcat
     7501 agccgaccag gccaatgagc cacagagcgc ggtcgtgcgc tttggcgagg ataggccgct
     7561 caggctggaa tccggcgtgc tgttcgcacc gcttcaggtc gcctatcaga cctacggcgc
     7621 actcaatgcg acaaagtcga acgccatcct catctgccac gccctgaccg gcgaccagca
     7681 tgtcgccaac gttcatcccg tcactggaaa gcccggctgg tgggaggaca tgatcggccc
     7741 cggccgattg atggatacgg accgctactt cgtgatctgc gccaacgtgc tgggcgcctg
     7801 catgggcaca tcggggccgg cctcgctcga tcccgcgacc ggccggccgt ttggtctcga
     7861 ctttccggtc gtcaccatcg gcgacatggt gaaggcgcag gagatgctgg tgacgcatct
     7921 cggcattgac cagctgctgt gcgtgatcgg cggctcgatg ggcggaatgc aggtcctgga
     7981 ctgggcatcg cggttccccg accgggtctt ctccgccatg ccgatcgcga cagctgcgaa
     8041 acactcggcg caaaacatcg cctttcacga agtcgggcgg cagtccgtga tggccgatcc
     8101 ggactggcgc ggcggacgct atctgctgga aggagcgcgg ccggaaaaag ggctcgccgt
     8161 cgcgcgcatg gccgcgcaca tcacctacct gtccgaacgc gcgctgcaac gcaagttcgg
     8221 acgcaacctg caggaccgcg ccgcgccgac cttttccttc gatgcggatt tccaggtgga
     8281 gagctacctg cgctaccagg gcgcgacctt cgttgaacgc ttcgacgcca attcctacct
     8341 ctatctcacc cgcgccatgg actatttcga tctggccgcg gaccatggcg gcgtgctggc
     8401 tgatgcgttc cggggcgcca gaacccgctt ctgcctgatc tcgtttacgt cagactggct
     8461 gtttcccacg gcggacagcc ggcagatcgt gcatgccctg aacgccgccg gcgcgtcggt
     8521 ctcgttcgtg gaaattgaga gcgacaaggg gcacgacgcc tttctgctcg acgaaccatc
     8581 gcttttagcc accatgaagg gctttctcga agccgccgcc agagcaaagg ggatcgcatg
     8641 agtcccgatg acatgagctt gcgcgagcag tcggcagcca tgcaggcccg agtcgacttc
     8701 ctgctggtcg cggacatggt ggagtccggc gcgcgcgtgc tcgatgtcgg ctgcggcgac
     8761 ggtgagttgc tggccctgct ggcccagtcc aaacaggtcg atgcgcgcgg catagagctg
     8821 tcccgcgaag gcgtcgccgg ctgcgtcgcc aaggggttgg cggtgattca gggcgatgcg
     8881 gacaccgacc tcgccgacta ccccaacgac tgtttcgatt acgtcatcct gtcgcagacg
     8941 ctgcaggcca cgcgcaatcc gcgcaaggtg ctggaacaga tgctgcgcat cggccggcgc
     9001 gccatcgtct cctttccaaa cttcggccat tggcgcatac gatcggggct gttctttcgg
     9061 gggcggatgc cgcgcaccga gaacctgccc tattcctggt acgacacgcc gaacatccac
     9121 ttctgcacga tcgccgactt cgtcgaactc gtcggcgacc tgccggccac aatggagaag
     9181 gccgtcgcgc tgaacgcctc cggctcgcag ctgggtttca acgcgccgtg gtggctgtgg
     9241 aacctcgttg gcgagcaggc cgttttcctg ctccggaggg ggtgaggcgc gcctagcgcc
     9301 ccttgagcgc ctggatcagc agcttctcag ctgtatgcag gcgctcgccc tgatactctg
     9361 cgccgttcgg gcacaaattg taggcccgca cgatccagtc gccgggcttg gccgccgtat
     9421 ccaggatatg catgcaccct gcggtggatt cgaaccgtcc cagaaatgcg ccgtcgccgc
     9481 ccagcacacg cgacacgatg gcggaattga ccaccgagtg caggaccagc agcgcgttgt
     9541 cccaggtctc atcctgcagc agtgtgtcga ccgccgcata aaccctggcc tgcatctctc
     9601 cgatggtttc cccgccgatg aatttcatgt ccgccggcac gcggggctgc gcgatcccga
     9661 gcagcacggc aggcaggtcg ggcgtggcaa cggaacgggt cgacccgttg cggatctccc
     9721 gcaactcggg aacctgcttc aaggttccgg caatcgctcc cgccgccatg acgcgctcgg
     9781 ctgtttctat cgtgcgcggc aggtgcgagg acatgatgcg atcgaacttc gcggcgccaa
     9841 tcgaggcgag gtagcggccc gccgcatcgg cctgcgcctt gcccttgtcc gacaggacga
     9901 ccagatcggc attggccgtg ggctggccgc tggcgtcaaa atagctgacg tcgccatggc
     9961 gcatgagcac gatacggcgg cgtttgggcg gctgccaggc cggaggcgac gcttgcgcct
    10021 gggcggcggg cgcggccggc gtctgcgact gcgcggagac cggcaggatc aggacgcatg
    10081 caaaaagcgc aatcgttaag atcgaacggc gcatggtaaa tcctttcaaa atctcgaacc
    10141 ggtgtgactg ttccgaaatg cgctgttgcc tgtcaatgcg cgtcgccccg cctccagcgg
    10201 cgcgaaattc tcgcggattt gggcctttcc ggcatgctta tcaaatcctt aacaaaatta
    10261 accgtggtaa atggttccca accgaacctt tgattttcgt ggttttttgt gttagaggat
    10321 gcgtacagtc gaaatggcga aacgccttgc cgtaaacctg actcccgaaa gaccttctct
    10381 ttgaacacgc ctgcccgttg cggaggcgaa gtggtctttg tcggcggatt tcatggcgcg
    10441 ggacgctgat cggatcgact gcccacgacc atccggcttt ctggccggca ggttcaatcg
    10501 aagcggattt cccaccgggt atccgcaaga cgaggttcgc gccccgttct agggcggagc
    10561 cgcagcgagg gacgagaatg agcgcgaaga agacgaccca gcgcgaaggg ttcaagaccg
    10621 gcgagcatat cgtgtatccg gcccatggcg tcggccgcat catggccatc gaggagcagg
    10681 aaattgcggg cttcaagctc gagctgttcg tcgtgacctt cgccaaggaa aaaatgaccc
    10741 tgcgcgtccc gacctcgaag atcgccgctg ttggcatgcg caagctttcg ggcggcgcgg
    10801 cgatcgagaa gtctttggag attctgaccg gccgcgcgcg catcaagcgc acgatgtgga
    10861 gccgccgcgc ccaggaatac gaggcgaaga tcaactcggg cgatctcatc gccaccgccg
    10921 aagtcgtgcg cgatctctac cggtcggaag cgcaacccga gcaatcctat tccgagcgcc
    10981 agctctacga gacagcgctc gcgcgcctgc tcgacgagat cgcagcggtt gagcagatca
    11041 ccgaaaccga ggccatgaag cgcatcgacg cgagcctggc gaagaatccg aagcgccggg
    11101 ccgcgagcga agcgagcgat gccgacgcag aggaagaggc tgcctgaggc tgcatacagg
    11161 acatgcaatc gcattctggg cccgggaaac cgggcccttt cttttcttga tggacgtcca
    11221 ctgccttgcg gccgccacag atgtaactat gttaagccgc aagatcgatc cggtcgacga
    11281 cgggaggggt tatgtcgccg ctgacgggag ttcgcaggca gttcgtgcgt gccgccatgg
    11341 aggcgccata cctcagccgg gaggaagagc atggactggc ggttgcatgg cgtgatcaca
    11401 aggatcgcgc cgccctggat cgcctgaccc aggcccatat gcgtctggcc atcgcgctgg
    11461 ccgcgaagtt ccgtaattat ggcctgccca tggccgatct gatccaggaa ggccatgtcg
    11521 gcctgatgga agcggccgcg cgtttcgagc cggatcgcga agtgcggttt tcgacctacg
    11581 cctcctggtg gattcgcgcc gcgatccagg actacgtgct caagaactgg tcgatcgtgc
    11641 gcggcggtac aagttcggcc cagaaagccc tgttcttcaa cctgcgccgg ctgcgtgcgc
    11701 agatcggtct gcggatctct cagggcgagc aactcgacct ccatgacgag attgctgccg
    11761 cggttggcgt gtcgcggcag gacgtcgtcg cgatggaggc gcgcatcggc gcttccgata
    11821 tctcccttga tgcgccggtg acggaagcgg acggagcggg cgccgccgag cggatggact
    11881 tcctggtttc ggagtcgccc ggtcccgacg aggcggtcag cgaactcatg gacagcgaac
    11941 ggcgggcggc ctggctgtcg caggcgttgg cgcagctctc ggaacgagag atgcacgtgg
    12001 tcaggacgcg ccggctggtc gaggacgagg atagcgcgac gctggaatct ctcgggacga
    12061 cacttggcgt ttcgaaggag cgcgtgcgtc agatcgagaa gcgtgcgctg gaaaaactca
    12121 aggcggcgct tgtacggctc aatccgggca tggcgcgcgg ctgaggcggg gcacgcaggc
    12181 tgtcaccccg gcggtcgcct caatcgacga ccgtcttgta ggtcaggcct ggcaccagtt
    12241 ctgcgccccg cgcgaggccg ttcagcagca ggaaccgttc cagtggccgg tcgataccgc
    12301 ccatgcgctg gctgaagtcc tctgcggtct cgccccgttt tgcggccacc acggcaatgc
    12361 ggagtggccg cgcccggctc tgctcctctt gcgacaggcg gcggaacgaa ttcgccgcgc
    12421 tgaagaagcg ctcgtccgtc gccggcgtaa agctccggga cgccaggatc aagcgataga
    12481 cggtcccacc ctggcgaact gcaaagatcc ggaaagtcca gtcggcgcca cgcgcgatac
    12541 ctgtcgccgc tggcaatccg ttgatcgtca gcgtctgcac atccgaaagc gtcaggttct
    12601 cgatgcggtt ctcgacgatg aaatcctcaa gcttcttgtc aggagcgacc ttcgcgctgt
    12661 cgagccgcag cgcctcctgg gcaccggcgg tcatcccgag cacggcctgc gaggaattct
    12721 ccaagaggaa cccgtccggg gccgtgaacg cgacaccgag cttcgggtgg aggaagttcc
    12781 gcccgcgcac gaagccctcg ctcgaatcct cgccgtagat catgccgttc aacgcctgca
    12841 tccagcgggc acgctcgctg gcgatcacgc ccggctcgaa gaaataccgt gccgccgcca
    12901 gggccgccgc tatgcggtcc ggcgttgtcg gatgcgtggc cgtgaactcc ggaccctcac
    12961 cgcgcgtctg gccctctgag gcacgttccc tgaactctga gctgcgtccg agcgccagca
    13021 gaaaccgatg cgccccgtgc ggatcgaacc ctgccccggc gagcgtccgc accccgatct
    13081 cgtcggcttc cagttcctgc tggcgagaaa agctggcgat ggcaacctgt gcctgatcgc
    13141 gaacgagctg ggcggcgccc ggattgctca gcacctcggc gcgcacgcgg ctgaccagga
    13201 ccgagcggcc ttccagttcc gcgcgaccct cggcgtggcg ggctgtcaca tgcgcaatct
    13261 cgtgggcaag caccgaggca acctctgaaa ggtcgttggc cagcgccagc aggccgcggg
    13321 tgacgtagag attgcccgag ggcaaggcga aagcgttgac gacggcggaa ttgaggatcg
    13381 taacgcggta cttctccgcc ggcttctcgg tgaccgcacg cagccgatcc gataggtcgt
    13441 cgagaatgcg ctggaccgcg ggggcgcgat actcgccgcc aaaagcctgc agcaaccgct
    13501 gatgctcgcg cccggcctgt gtttctcccc cggaaacgcg cggcgctgac gcaggcaatt
    13561 cgacctgcac agtcgcctgt ggcccgagga tgagaagatc ggtgcacccg gccaaggcaa
    13621 acagcagtgt cagcgcagca ggccgcaggc cgggccgaat gccaggctga cacttcccaa
    13681 tacctgtctg cgacccagcc aagcctactc cagaacttca acagccatcg gccccgtcag
    13741 gtggagccgg ggtgtgggca cctgcgacac gacgccacgc acccgaagcc gtttacctga
    13801 aaatgtggcg ggatcgagcc cttccgcgcg ccaacgtgaa agatcgcggt tcctgaccat
    13861 gatgaacggc gtggcggagc gcgtgccctc gaaattgacg aacgtcgccg accgcccggt
    13921 gcgcacggag cgcacgcgtg cctcgaacac ggcccggccc gacagggtct ggcgcgcgac
    13981 gaccctcgat actcgcatcg ttcgtctgga cagcggcgac cacaggccca gaccggcagc
    14041 gcgggcggca ttctccgcgg cgagcaaccg gggcctgcag gggtccgtcg ccgcttcagg
    14101 ccagtgccgg accagtccga gagccaccag ggtctcctgc acccagagcg gttgcggcgg
    14161 accatcgggg gtggccagca cgagattgcc ggaaagccgg ccccagcgat ccgacttgcc
    14221 tgcggaatcg acccgggcct gcgttccggc ggcgagccgc agcagcacca tccgcaaccg
    14281 gtcgcgccag ccctgcctgt cgggcgatgg ccagtcgatc cccgacaggc gaacgatccg
    14341 cccgtccgcc agactgactt ccccggtctg cgctaccgcg atcaccgtcg atgccggccc
    14401 catgggagcg ggacaggcct gaagcggcaa ggccagcacg accagggcga gcgccaagag
    14461 aacaagcgca cagcgctttg accgggtgcc tgcgctcccg ctactgtctg tgccgcacgc
    14521 cacgcacagg attgccatcc atgacctcga acgcccccgc cgcgccgctc gcccgctatg
    14581 ccgcctggaa acgccggggg gacttcatct tcatgtccgg catcattgcg gtggatccgg
    14641 cactgggcaa ggtggtcatg ggatatgacg acatcccggt cgaagcgcag gcgcttctgg
    14701 gaaggactgg cgagatgtcg gtcgacgtca aggagggacc gatcctggcg caaagctggt
    14761 acgtcctcga caagatccgc cagaccatcg agggttgcgg cggcagcatg ggcgacgtgt
    14821 tcaagctcgt ccagtacttc cgcgatctgc gcgactatcc gcactataac cgcgtgcgca
    14881 agctgttcta cccgaccgac ccgccggtga gcaccgtggt cgaggtttcg gcgatgttgc
    14941 cgaacgaaca catcctggtc gaagttgagg caacggcatg gctgccattg atttaaatca
    15001 aaaccgtatt tgcctacctg cggcaatgac gtgtgacgtt tctcaagttg aaacgcacaa
    15061 gccagagcct gccggcccca acgcctgagg gggacgctaa gccgacatcg ctccatcaga
    15121 agaccagaaa ctctcattgg ggctgtcatg aagcgtgtcc tcctcgccgc cgcaagcgct
    15181 gcggctctcc tcggttctca ggccttcgcg gcggatgcgc cgatgaagcc tggcgcgatg
    15241 ccaatcgaag cgttcaatcc ctggatggtg cgtctgcgcg cccttgcggt cgttccgcag
    15301 gaggacgcca gcctgagcac cgccgccgga ccgatcattg gcggcaaggt cgggatttcc
    15361 aactcggtcg ttcccgagct cgacatcacc tacttcttca atcggaactt tgctgtggaa
    15421 ctgatcctcg gtaccacgcc gcacaaggtg aagggcgcgg gcgcccttgc gggtgttggc
    15481 ccgatcggtc gcgcctggtt gctgcccccg acgctgaccg ctcagtatca cttctacctg
    15541 accgactcga tcaagccgta tgtcggcgcg ggcatcaact acacgatctt ctacaatgag
    15601 aagtcatccg gcgtgttcac cggaaccaag ttcgatctca aggatacgtt cggcctggcc
    15661 gtgcaggccg gcgtcgacat catgatcaac aagaactggg gcttcaacgt cgacgtgaag
    15721 aagatttggc ttgagccgaa tgtcagcgtc ggcagcggcg ctgtgaccgg gaaggtcaag
    15781 atcgacccct ggctgatcgg cgcgggcatt acctacaagt tctgacccga ccgtgatcat
    15841 ccctctcgaa ggccgcctca gggcggcctt cttgctttgg ctgccgcaag atcaggagcg
    15901 ggcgaggacc ggatctagtt cgcgatagtc cggcaaggat cggtcgatgg gcttgccacg
    15961 gcgcaagacg gtgcgatctg actgtggacg cgacagcagt tcgctccagt gtcttgcccg
    16021 gaacaggacg aggtccgccg gggcgccgac ccccagatca acgccccctc ccaggaagct
    16081 ggcgggcgtc cgcgtcaccg tcctgatcca gtctggcgca gaatgatcga aatgcaggat
    16141 gcgcgttgct tcgcgataga cttcgaggac atcgagatcg ccataggcat agaacggatc
    16201 gcgcgtgttg tctgaggcga ccatcacctc gatgccgcgc gccttcattt catgcagcag
    16261 cgtgacgccg cgccgtcgcg gcgtgcggcc cggcacgcgg tcctgcaggt acatgttgca
    16321 catcggcagc gacaccacag cgatgccggc gcgcgccatc aagtccatcg tgcggtcggc
    16381 ctcgtcctcg gcctgcatcg acagcgaaca gcaatgtcca accagtatgc gtccgggaaa
    16441 ccacaggctc aacgccattt ccgcgatgac gcgcagggat tcagccgtcg gatcgagcgt
    16501 ctcgtcgaca tggaaatcaa gatcccagcc cttttcccgc gcgagcagga acaaccgctc
    16561 tagccctggc cgcagcgccg ggcccatgta ggtgacagcg cccatcacat gcccatgtcg
    16621 gtccaccatg tcggagacct cggccatatg cgcatcgtcg agcgcgagct caatgccgaa
    16681 gagcggcgat gcgtccagtg cgatccgtcc cgcccatgtc tcgcgcattg ccgcgaacac
    16741 gggccacgag attgcggtct gccctggacg cgagtcgata tgcgtgcgga ttgccgaggt
    16801 gccgtgcgca taggcgcagc gcagcgagaa ctccatgcgg gcagccacgt cttctgcggt
    16861 ccaactcgcc tgccggtccg ccaggaccgc attgagcgca cccatgaacg tgccgtcggg
    16921 attgggccgg cgcggccaga tgtgcccctt gtccagatgc gtgtgcacat cggcaaagca
    16981 cggcaggatc atgccctggt cgagatcgag ggtcggggtc gtgccgtccg caggctccac
    17041 acccgcagcg acgatggatg cgatcttgcc ttcggccagg accaagtcgc accgcgccag
    17101 tccatcgggc gttgccagca gcagctgccc ggactgaacc aggcaggccg gaagggtggc
    17161 gttgacgatg cgaagccgcg cggcatgcgg cacctttgcg aacccggccg tcacgtcagt
    17221 tctcccgctt cacggcgctt tcgtgccagt gccgcagcaa gaggtgcgta acccagctga
    17281 tgatcaggaa gatggcgatc ccggtcatcg agatgaccag cagggcagcg aacatgcgcg
    17341 gaatcttgag ctgatagccc gattccagaa tgcgataggc caaccccgaa gcggacccgc
    17401 cggttcccgc cacgaactcg gccaccaccg cgccgatcag cgcaagcccg ccggagatgc
    17461 gcagaccgcc gaggaaatac ggcaacgccg ccggcagctt cagatagcgc agcgtctgcc
    17521 accgcgaggc gccgtacatc tggaacaggt tgatcaggtt gtgatcggcc gagttgaggc
    17581 cgaggatcgt gttggaaagg atcgggaaga aggccacgat ccaggcgcag atcaggagcg
    17641 acagcgtgac gttgttgtcg gtccagataa tgatgagggg cgcgatcgag acgaccggcg
    17701 tcacctgcag gatcaccgca tagggcagca acgatttttc cagccacttc gactgggtga
    17761 acagtacggc gagcgtcacg ccgaagaaca ccgcagcagc cagagcctga agggtgatgg
    17821 tcagcgtcac ctgcagggac gtcgccagca gggcccagtc ggcgacgagg gtctggccga
    17881 tcagtttcgg ccccggcagg acgtagttcg gaatctggta ggcgttgacc gcccattccc
    17941 aggcgaacag cgccagaatg ccgatgccaa ggggcgccag gatgccgggc caggtcgagg
    18001 cagcaagacc gagaatcttg cgctcttcgg caaagaccgg gcgggcttcg gcgtcggttc
    18061 cgtcatgcgg gacggagggc agcgagggag cgctcatagg gcaatcgcct tcttcaggct
    18121 gtccgaagcg atgcggcaca actgcgcata gtcggccgag gtgcggaact gatcgtcgcg
    18181 cggataggga ccacccacgg caaggtccgc catgacccga cccgggcgcg ccgccatgac
    18241 cacgatgcgc tgcgaaagat agacactttc gaacaccgaa tgcgtgacga aaaccgccgt
    18301 gaaccggcgc tcccaccaca gtttcaagag atcgtcgttg agccggtggc gggtgatctc
    18361 gtccagcgcc gcgaatggct cgtccatcag caggatttcc ggccgggtga cgagcgcgcg
    18421 ggcgatggaa acgcgcatct tcatgccacc ggacaattcg cgcggatagg acttctcgaa
    18481 ccccttgagg ccgaccagcg cgagcatttc cgccgaccgc gcttccgcgt ccgcccgggc
    18541 gacgcccttc aacttcaacg gcagcatgac gttgccgagg gcattcgccc acggcatcag
    18601 cgttggatcc tggaagacaa aacccagttc atggtcgacc gacggccagt caatagtccc
    18661 cgcgctcggg gaaccgaggc cggccagcat gcgcaacagg gtggacttgc cgcagcccga
    18721 aggcccgagc agcgacagga actcgccgtg cctcaagtcc aggtttacgt catggacggc
    18781 cagcgttccg ttggcgaact gcttggaaac gccacgaatg gagcagacga caggcgcggc
    18841 cttcgtcgcg ccttcctttg tcgcaatgtc ggtcaagtcg gtcaggcctt cccgataccc
    18901 ttgttgacga aggcgaggct gtaggccttg gtgatgtcga gccccttggg gaagacgtcg
    18961 acagccgcca tggtctcgta gaagcgcttc cagcgcgcgt ccgtcatggc gccgacgccg
    19021 agcgtcaggg catcgccgga ctggatgatg ccgcgctcgt tcatcacctt gaccgcgtat
    19081 tcgatcttct cgtcgtccat ttccggattg tcttttttga tcagatcgtt ggcggccttg
    19141 aagtattccc ccttcttcat ataggcggcc cagctctcga aggtcgcatc acagaagcgc
    19201 tgcaccacgt ccttcttctc ctcgaccatc ttccgggaga tgttgatggt cgtgttgtaa
    19261 ttctcaaagc cggcgtcggc gatgagatgc acaaccgggt tcaccccgcc cttctggatg
    19321 gcgaagggct ccgaggacag gaagccttgc tgcgacaggt tcttgtcggc caggaaaggt
    19381 gccatgttga acgtgtaggg gcgaatctga tcgtcggcat agccgaactt cgcccggagg
    19441 aacggccagt acgacgtgcg gcccaccgcg ccgatgagga tcggcttgcc cttcagcgcc
    19501 gcaagcgaat catgccccat gttggggtgc gagatcagca cctgcgggtc cttctggaag
    19561 atggaggcga tgcacaggaa aggcaggttc tcctgcacat agcggatggc atcgaaggag
    19621 ttcgacatga tcatgtcgac gcgcccggcc agcagcagct gcgacgggct catctgcggc
    19681 ccgcccatgc ggatgtccgc gtcgatgccg tacttcttgt agatgcccga ggcgacgccg
    19741 taatagaaac cgccgtgttc ggcctgagcg cgccagttcg tctggtagct gaccttgtcg
    19801 agcgtctgag cgttcacctt cgctcccggc atgggaatca gagtggatgc ggcgctgcca
    19861 ccaatcaggc caagcgcagc gcggcgattg aggcgatact tcatcatggt catggttggt
    19921 cccctcgtac tggccaatgc gagcaccgga atgcaaccgg cgtgccggca cgcatcccct
    19981 cccggtcccg ttcttagacg atctcgcgat caagtcatag ccgcgctgcc caggcggaag
    20041 gcaaaaggcc cacgaaatcc acaaaacgga tgcggggcac cgggtattgt cgcgcaatgg
    20101 tattgctggc ctgccacgtg cttcgtatgg tccccaccgc acagtcagga gagccgagat
    20161 gaaccgcccg gaacgtatcg atgccgcacc tgcctacgac atcgacgccc tgatcgccga
    20221 gctgaaggac attccccacg aaaccgaacg gaccagcgtg cgccgccgct cgcgcgactt
    20281 cttctggtat tcgcccgtcc tcaaccggca gctgcatggt cttgcggcgg acgtcatctt
    20341 catgccgcgc gacgagaagg acgtggtcgc gatcgccggg gcctgcgccc gcaggcgcat
    20401 cccggtgacg ccgcgcggcg gcgcgaccgg caattacgga caggccgtgc cgctgaaagg
    20461 cggcgtgctt ttggacatga acggcctcaa caaggtgctg tggcacaaac caggcgtggt
    20521 gcgggcacag gccggcgcga tgctgcacga aatcgactat ctgacccggc catcaggctg
    20581 ggaattgagg ctgcacccct caaccaaacg gatggcgacc atcggcggtt tcgtggccgg
    20641 cggctcgggc ggcatcggct cggtaaccta tggcggcctg cgcgaacccg gcaatgtgat
    20701 gggcgctcgc atcgtgaccg tcgaggagac gccgcgcgtc ctcgaactgc gggccgatgc
    20761 ggcgcagaag atcaaccgcg cctatggcac gaccggcatc atcgtcgagc ttgagatgcc
    20821 gctcgcgccg gcctggacct gggtcgatct gatcgtcggt tgcgaggatt tcgagaccgc
    20881 gatgcaggtc ggctacgacg tggcccatgc cgacggcatc gtgaagaagg tcgtgacgct
    20941 gctcgatacg ccgatccctg actatttcgg tccgatcgcc gcgcagtgtc cgaaaggcaa
    21001 gccggtgctg atctgcatga tcgccgaacc gttcgtcgag tatttcaagg cgctggtcgg
    21061 agcgcgcggc acggtgacct acgagcaggt ttcggacgac acgcccggat cggttcccct
    21121 gtacgaatac acttggaacc actcgacgct gcagtggctc aagacggacc gctcgattac
    21181 ctacctgcaa aacctctatc cgcatgaccg gctgttcgag agcgtgcggc agatgcgcga
    21241 catgttcctc ggcgaggtgc actatcacct cgaattcatc cgctatgctg gcaagctctc
    21301 ctgctcggcg ctgccgatcg tgcgctatac caccgaggag cgactcaacg agatcattgc
    21361 cctgcatgag cagcatggcg tgatgatcgc caatccgcat gtctacacgc tggaggaagg
    21421 cagccggcac aagtgggtcg acgccgacca gatgaacttc aaacacaaca ccgatccgct
    21481 tgggctgctc aatccgggca agatgaagag tttcgtgccg cgctgaaccc atgcgtgtcc
    21541 tttatcttta cgcccatcct gttccggaaa gcttccacgg cgcgatccgc gccgaggcac
    21601 tcgccggcct tgccgccgcc gggcacgagg tcgacctgat cgacctctac gcggacggtt
    21661 tcgatccggt gatcaccgcc gaccagagac gctgctacca cgacaccacg cgcaaccgcg
    21721 ccggcatgga agactacatc gaccgcctca accgggcgca agcgctggtg gtgcagttcc
    21781 cgacctggac cttcgggcct cccgcgattc tcaaggggtt cttcgaccgg atcatgatcc
    21841 ccggcgtcgc cttcgacctg agcgatccgg ccaatgtcag gcccctgctc ggccatatcc
    21901 gccgcatcca cggcatcgtc acctatggcc ggcagcgctg ggtggcgctg gtggtgggcg
    21961 acggtccgcg caataccgtg acacgctatc tgcgccgcct gatcggctgg cgcgcgagaa
    22021 cgggctatga cgccctctac cacatgaacg tggcgacgga tgccgaccgg aagcattttc
    22081 tggcccgcgt gcgtcgctcg atggaaaacc tgtgactgga tcagccgatg cgcaagctct
    22141 tctggggtga cctgacaacc gaggattttc gcagcctcga tccggaaaag accatcgcgg
    22201 tgatgccgat cgccgcgatt gagcagcacg gaccccacct gccggtcggc gtcgataccg
    22261 agatcgccaa cggcatgatc cgcgagacga tccgcctgct gccggaggat atggcgatcc
    22321 tgattcttcc gacgcaatgc gtcggcaagt cgaacgagca catccgctcg cccggcacgc
    22381 tcaccctgac cgccgagacg gcgattcgcg cctggacgga gatcggcgag agcgtgcagc
    22441 gcgccggcat ccgtaaactg gtgatggtca attcccacgg cggcaatgtt gattgcatcg
    22501 gcatcgtcgc gcgcgaattg cgcgtgcggt tccagatgct cgcggtgcac tgcgcctgga
    22561 gccggtttgg cgctccggac ggcatgttcg gcgagacgga gaacgccgtc ggcattcacg
    22621 ccggcgacaa ggaaacctcg ctcatgctcc atttccgccc ggacctggtg aagatggaca
    22681 aggcgcagaa cttcgcgccg acgacgatcg agatcgcgac gagcttttcg ctcctgcgac
    22741 ccacgggatt taccgccttc ggctggctgg cacaggacct gcacccttcg ggcgcggccg
    22801 gggacgcgtc ccgcgccacc gccgaaaagg ggcgcgcgct cgccgaatac gaggcccgga
    22861 agttcatcga actgctgcgc gacgtggaac gcttctcgct ggcccgcctt gcctgagcct
    22921 gccaaaactc ggtgcttctc aaagccccgg caagcggtgt atcacggcgt catgcggttc
    22981 cgtagctcag caggatagag cagcggtttc ctaaaccgaa ggtcacaggt tcgaatcctg
    23041 tcgggaccgc cagcgcatct ctatttattt gattttactt ctattttagg agattttccg
    23101 aactttttga ccctggtcag gggcagcttc gttcgcggta cgacccttcc ctcgactgac
    23161 tgctgatcga gccaggcccg cttgatcggc ctcgcgtgta tagcgctcta gccgtagcac
    23221 gttccgccat cgacgatgag tgtctgtccg gtgatcgccc gggcatcgga ggaacagagg
    23281 aacagggccg cggccgcgat gtcgcgcggg accagttccc gacggatgga ttgcgcggcg
    23341 agattggcgg cgcgtttctc cggcgtcgca tggtctgcga tctggcgggc tgtcatcacc
    23401 cagcccggcg cgatggcgtt gacgcggatg cccgccggac cgagttcgcg cgagagcgaa
    23461 cgggtgaacc cttcgacagc ggatttcgcg gtcgcatagg cgatcatgtt ggtcgaggcc
    23521 ttcatccagg cggtcgagcc catggtcagg atcaccccgc ggcccgcttc acgcatggcc
    23581 ggcgcgacgg cctgcgccgc gaagatgtag tggcgcagat tgacggcgat gcgctcatcc
    23641 cattcggaga cctcgagccg gtcgaagtcg tgccgagcgt catgcgcggc attgttgacg
    23701 agaatggcgg gcgggccgac acgcccggcc agacgggcca aggctagccg catcgctgcg
    23761 atgtctcgca gatcgacctg ttccgccgcg agttccgggc actcggacgt gaacgcctgg
    23821 agggccccgg catcgcgatc gagaatgccg acgcgcgagc cttgcacgag gaatgcctcc
    23881 gccatggccc ggccgatgcc acttgctccg ccggtgatga cgaccggctg atccttcaga
    23941 tccgggtagc gggcgagcgg ctgcgacatc ggtcagcctg caaagagact gggaggccgc
    24001 ccgcgtacgc cgacatcgac ggcgaggatg ccaccggaga acggcgctgc agcgaggatt
    24061 ttggcgggaa cccgcacgcg tgccgaggtg atgaacagcg tcgtcagggc aggcccgccg
    24121 aacgcaacgc tggtggggcg gggaaccggc acgcgcaact cgcgcatcag gcgcccctgc
    24181 gggtcgaagc gccgcacgca ccagccatcc cagagggcgg accagacgca gccttccgaa
    24241 tcgaccgtca ggccatcggg tcgaccttcg cccggatcga tccgcacgaa gtcccggcgc
    24301 ttgcccagcg tgcccgtctc catgtcgaaa tcataggcgt agatcgtccc ggcactcgaa
    24361 tcggtaaagt agaacacgcg cccgtcgggg ctccagtcga ggccgttcgc cacggtgaag
    24421 gggtgatcca tgcgttgcca ggcatgattg ggatcgatgc ggtagagcgc gccggacttg
    24481 cgcgcagcgt cgaggctcat cgtgcctgcc caaagccgcc cggcgggatc gcacttcgca
    24541 tcgttgaagc ggttttccgg ctgcatcgct tcaggatcga cccgccggac cagttgacgt
    24601 gaagcgaaat caagttcctc gacgccgtcc gaggtggtca cgatcaggcc gccgccacgc
    24661 cgtccgatca ccgaggagac gaggcgcggc agcgggcagc tttcattgat gccgccggcg
    24721 ggatcgaacc ggtgaacagc aggcgccaga atatccaccc agtagagttt ttgctcggcg
    24781 atcgaccaaa acggtccttc cgcaaggctc gcgctccacg gaagcacgca ttccacatcc
    24841 gggtcggcgc cgcgcaccgg cgcgctgcgc gaatggatac tgacagaggg cgcgccaaca
    24901 ttccctgaga tcagccgcgc ggcttccatc aggtcacgtc cgaaactgtg caggcgatcc
    24961 tcgggattgc gcgaggaggg tccgaacatg ccgatcgcgc cgatggcttt gccggtatgg
    25021 tcgaagatcg gcgcggccac cgagacgacg ccgtcgacat gctcgccatt ggaaatcgca
    25081 tagccgcgtg cgcgggtcag cgcgagatcg gccagtagca gttccggatc aaccagcgtg
    25141 gtcgtcgtga agcgatccgt cgggatgcga tgcaggatcg cccgctggtc atgcggcgtg
    25201 gaaaaggcca gcagcgcctt gccgaccgcc gtgcaatgca ggggtgcacg ccgcccgacc
    25261 tcgatacgga agccgaacgg cccgccctgg ttgcgttgct cgatatagac gatctggtcg
    25321 ttgtcgagcg tgccgatcgc aaccgtttcg cgcgcctcga gtgccagcct ttcgagctgc
    25381 ggggcagccg cgccgcgaag atcgaacgtt tcccagacgc gatgcgccag ctcgaacagg
    25441 cgattgccca accggtaggt gttgtcgcgt tcctcgtgtc gcaccaggcg gaaagcgacg
    25501 agcgtgtgaa gcatgcgcgc gagggtcccc ttcggcagac cggtcgcgac ctggagatcc
    25561 ttgaagcgtg cgggtgtctc gcgctctccg agcacgtcaa gcagatagag ccccttggcc
    25621 agcgccgccg cgccgccggg caattcccgc tcatcgggtc tcaggacttc tgcttcgctc
    25681 gccatgcgac ggctgcctcc tccagcatcc ggtcaaaccg ataggacggc tcgaacccgg
    25741 tgatgttccg gaaacgggcg ttcgaggttt cgtagaacac gccggctccc ggcaagtcca
    25801 caacccgcac gggcaggcca gtgatcgcag ccatcttcgg caggacctcg gcgaaatcga
    25861 ccgcttgcgt cgcgccgaga ttgcaggttt cgcccagcat tcttggcgtt gcgagcgaag
    25921 ccagcaggcc ctccaccatg tcgcgcgtct cggtgatcat catgcggaag ggccgcccct
    25981 tctcgttgcg cgcgagcacg tgggcgggtg tgccgtcatc gacagcgcga agcgctgcga
    26041 ccgccgcagc attgccgagg gcctgctgct gggcgatgcg tggctgcagg aagaagcgcg
    26101 gaccggagaa gaagctgccg ggatcaagca gttccgccgc atcctgcgtg tgagagaagc
    26161 gaaggatcgt gaagggcagg ccgtggctgc gcccggccca gcgcaccagc tcctcgccga
    26221 ggagcttcgt gaggccatag gtcgagcgcg gctccgtcgg atgggtttca tccaccggca
    26281 gaaaagccgg cgcgccttcg ggatagacct cgcccgaaga tgcgaagaga aagcgctcga
    26341 ccttcgcgcc gaccgccgcc tccagcacca agcgcgtgcc ttcgacattt gcggcgaaga
    26401 ggcgatcctg atcctgcggc agccaggaca tgaaggcgcc gagatggaac acggcgcgca
    26461 ccccactcat ggcccggccc acggcctcgc gatcatcgaa ggcaccgagc acctcgtcga
    26521 aacgatcatg cgtcaggaca gtggagcgca ggtcgagtcc gcgcaccgtc tcccccctgg
    26581 cgagaaggcg cgcgacgagg ctccggccga tccgtcccgc actgccggtg acaagaatgg
    26641 tcatgctcgt tcaccggatc gcgcggccgg aggccggatc aaacacatgc agccgggcgg
    26701 ggtcgaacga gagggtcaat tcctcgccga cggtgatcgg tccgctattc atcagtcggg
    26761 cgacaaggcg atcgccgcgc cgttcctcat cggttcggcc gatggggttc accagccggt
    26821 ttgcctgcgg agcttcgaaa tagacgtact tctcggagcc gaggctctcg accagcatcg
    26881 gccgcgtcgt gaaggtcacg gggccgggtc cggctgagaa atgttccggg cgcagtcccg
    26941 ccgtgatcgt accctcagct gccggaggca agggcacgtc ctgatcgaag agccgaatgc
    27001 gtccggctct cgtctcggcc ttgaggaagt tcatgccggg tgaaccgatg aatccggcga
    27061 cgaacaggtt cgccggctgc gcaaacatgg tctcgggatc ggcgatctgc tggacctcgc
    27121 cgtccttcat gatgacgatg cggtttgcca tggtcatcgc ctcgatctgg tcgtgggtga
    27181 catagatcgt cgtgacgccc agccgctggt gcagcgcgcc gatttcggcc cgcatctgca
    27241 cacgcagctt ggcatcaagg ttcgagagcg gttcgtccat caggaaggcc tggggctcgc
    27301 gcacgatggc gcgccccatc gccacgcgct ggcgctgccc gccggaaagc tggcgcggcc
    27361 gtcgcgccag caggtgctcg acgtcaagaa tgcgtgcggc ctcttgcacg cgcctggcga
    27421 taatgccggc cggcgtgccg cgcatcttca ggccgaagcc catgttcgcc gccaccgtga
    27481 gatgcggata gagcgcataa ttctgaaaaa ccatcgcgat gtcgcgatcg cccggagcga
    27541 ggtcggtcac gtcgcgtccg ccgatgcgaa cgacgccgct cgtcgcctct tcgaggcctg
    27601 cgaggatttt gagcatcgtc gacttcccgc agcccgacgg gccgacgaga acgacgaatt
    27661 ccccgtccgc gacctcaagg gagaaatcct tgagcacggt gagcgggccg taactcttgc
    27721 gcacgcgatc aatgacgatt tcagccatga caatccgttg ccgccctcac ttttcagaac
    27781 cggctgtcag gccgccgacg agatagcgtt cgaggaagag gtagatcgcg aggatcggca
    27841 gcgcgacgag gatcgctgcg gcggaaatgt ccgcccagtt cgagatgtac tcgcccttca
    27901 tcgtggtgat accgagattg gcggtccagt tgccctgtgc gttggtgagg aaggtccgcg
    27961 cataggcgta gtccgaccaa ctcagcacga agccgtagag cgcagtcgcg gccaggccgg
    28021 gcgcggagac aggcaggatc acccggaaca atgcgcccag cggcgagcag ccatcgacca
    28081 ttgccgcctg ttccagttcg cgcggaatgg tgtcgaaata gcctttcagc atccaggtcg
    28141 tgaacgggat gatgagcgtc gcatgggcga gaatgattga ccacatcgag ccgatcagcc
    28201 cggtcatccg gaagatgcca aagagcggga tcaccagcaa tgtggcaggc agcatcttgg
    28261 cagtgaggat gaagaggccg aggctgtctc cgccacgtac ccggaagcgc gagagcgagt
    28321 agccggcgag caccgagaca accatcgaga tcgtcaccgc gcccatcgcg gccagagccg
    28381 aattggacag ccagagcagg atcgggcgct cctcgaggat cttgccgaat acgctccagc
    28441 gcggttcttc cggccagaat ttcgggggaa gttggcgcag ctggtcgggt tgcgacagcg
    28501 cggtgacgat gagccagtaa atcgggaaga tcaggatcgc gcaggtcagg atgacgctgg
    28561 catagagggt gaggcgggtg gcgaggctct gtttcattcg cgcctcacag ggtgtcaggc
    28621 gcgcggcgca tcatcagctt cgccgcgatc acgcagatga caagcgttac cacgccgaca
    28681 gccgaggcat agcccatgcg gaagaagctg aaggcctgtt cgtaggtcat gatcgacagc
    28741 gtctgcgtcg ctttcagggg cccgccgccg gtcagcacgt agatgatcga gaaatcccgg
    28801 aagatccaga gcatcaccag gaccgtggtc acgcccagga cggggcgcag gttcggaagc
    28861 gtgatgtaga tcagccgctg cagggcgttg gcgccgtcca cgcgagcggc ggcatagtaa
    28921 tcctcgggga tcgattgcag gccggcgagg cacatgatcg agacgaaggg gtagcccttc
    28981 cagatcatcg tcgtcgccac caccgcgaag gccgctgtgg ggtccgagaa ccagttgatc
    29041 gtcttgtcaa tgaagcccag cgacaggaag atccagttca tcagcccgaa ggaggagtcg
    29101 aagatccagg cgaagatgac gaccgcaatc acctcgggca cggcccaggg catagccacg
    29161 agcaggcgtg ccagcgagcg gcccttgaag cggttgttca ccagcagcgc cgtgccaagc
    29221 cccagcaggc agcatgccgt ggtgacgatg aggatgagtt tcagcgtcac ccagcaggca
    29281 ttccagaaat ccggcgaacc gaagagccgc tcgtagttcg cggtggtgaa ggggcggcgc
    29341 ggctgatcga gccgcgcaat cccgacattc tggaacgaga gatcgaccga gacgaacagc
    29401 ggatagacga tgatcgccgc gatcagcagc acggcgggcg ccagcaggat gtagccgagc
    29461 cagtgttgcc ggctcggccg cgcaagatcc agactgaagc gtccgcccgc catcacatca
    29521 ctgcttctgg attgcctcgg ccttcgcctg catggtcgcg gcgacagccc tggggtcgag
    29581 atcctggatg atcatgcgct gcgcttcttc catgatcatc ttggcgaact cgttgtactg
    29641 gatttccagc cccttcggga tgcgatccac accagccttc gaagcggcct gcgcggtctg
    29701 gaccagcaga tcgaagtgcg gggtcgcagc cttcgcgcct tcggtcttgg cgcgtgggga
    29761 aggaggggtg gacgccgcga gagtcgcgta atcggtctgg aacttgtccg acgtggcgat
    29821 cgcgatgaaa tcccagacga gcttctttgt gtcggccgga atttcgctgg ccatccctag
    29881 gatgttcgag gaaccgccga gcggcgggtt gaagggcggg gcggcgatct tgatctgatc
    29941 cttcgccttg ccgcgctgga tgatgggata gagccacggt ccgtcgagct tcatggcgat
    30001 gcggccatca gcgaacatct ggcgcgtctc gcctgcggag agatcgcgcg gggtgagcga
    30061 gttcttcacg acgtccttcc agcgcgaaag gccctcgatc atctgcgggg tgttgatggt
    30121 caccttgcca ttggcatcgg tccagcggcc gccggcatcg agcgtgtagt tcatcagctc
    30181 ggagaggtat tgcccgccgc cgcccttggt ctcatgcccg gtgccgaact ggtcggggat
    30241 gccgtcgcca ttggtgtcct tggtgagctt gcgtgccgcg gcgacatact ccgcccaatt
    30301 ggtcggcggg ctaagtcctt cttttttcag catgtcttcg ttgtaggcga agatgttgcc
    30361 gaaatagagc atcatgacgc agacgggctt gcccttccac tgacaggagg attgcccggc
    30421 ccagccatcg agattgagcc cggacgcctt gatgtagggg gagagatctt caagccaccc
    30481 ctgatcggcg aatttctgga attcgaacga ggcaagatgc acgatctcag ggggtttgcc
    30541 gcccgcgaaa agcgttgtca tcgtgtcggc ataggcgccg cgctcgacct tggtccactc
    30601 gaccttcacg ccgggattct tcgtctcgaa ttccttgatg acgctggccc accaatcccc
    30661 cacgccgcgt tcgtccttct gccaggagac gaactttagc gtcttggtct gggcgagcgc
    30721 gggcggcgcg agcgtggaaa acgcaagcgc cgccacgcca ccgacaatca gcgatttcaa
    30781 tcggttcctc atgtcttcct ccggaattgc ggctctggcc gtcattgttc aaggttgcgc
    30841 aaggcttccg ccgaggggcg gaccccaagg cccggtcctg gcggcaggtg atattcgccc
    30901 gcctcgcaga tgatttcgcc atcgagatag cggcggttct gcccgacgat cgaatgctgg
    30961 aattcgtggc aggtcacttg cggggcagcg gcactggcat gcaggcttgc ggcgaggaaa
    31021 atgccgaagc ctatcgtcgc gtgcggaatg atttcggcat ggtgcacctg cgcgagcgtg
    31081 gcgatgcgca tgaattcggt gatgcccttg tggcccatct ccggttggag gatcgcgcag
    31141 gcccgacgcg agagccgcgc cgccgcatcg tgagcggtgc gccattcctc acccgccgcg
    31201 acagggcagg agacgccctg cgccacctcc gcaaggcccg cgatgtcctc cggcgcgacc
    31261 ggcgcctcgg cgaaccacag gccatgcgcc tccatggcgt ggatcatgga cttggcctga
    31321 gcggcattct gcgcccaatg catgtcgcag gcgatccgcg ccgaggggcc gagccgttca
    31381 cgcagggtcg cgatctcggt caccacgccg tcatccgcga cgggggaggc gaacttgaag
    31441 ctgtcaaagc ccttcgcctg ccatttcgcc gcgaggtcgg cgcgagcctg tcgtgtgggc
    31501 tcgggcaagc cggagacata ggccggaatg cgctccagac gctgaccgcc gagcagcttg
    31561 cacagcggct ggcccgcgat ctgcgccgcg atgtcccaca gcgcgatgtc gattgcggcg
    31621 agtgcatcga ggtagtagcc gccggtatag ccccgcacgc gcatcaggtt atacaagtca
    31681 tcgtggatcg cgcttgcgtc gaaggggtcc cgtcccacca cgaaggcggc gagaagatcg
    31741 gtgatgatct ccatggtcgc acgcggggcg acgatgccat aggtctcgcc ccagccgatc
    31801 acgccctgcc tcgtggtgat gcgcacgagc acgttgcgat cgaaattcgg gtaaaccgtc
    31861 ttgttgccgc gtcgcacgaa atagccgcgc tcgttcacct cctcgccggg ccgcagggtg
    31921 ccgagatagg gcgtctcccg cgtcacggtg agcatgaagg cttcaacgcg ggcaattctg
    31981 tcgctcatgc cgcgaccctg gccagagggg ctgcgacgag aaggtcggcg atgcggtcca
    32041 tcagggcctg gatttccgcg atatcgcgtg catcgaaagg cgggatcctc gcccgcacca
    32101 tgtcgttgtc gaggatgccg cgcagcatca gcacgcgctt ggtcagccgc atgcgcgcat
    32161 ggctctggat catcagcagc ggcagggtcc gttcgtagag cgaccgcgct tccgcctcgc
    32221 gaccggcctt gagcgcttgc cacatgcggg catggatgtc ggcaatttca accgccggca
    32281 tggccccgca ggccccgcgc aggaactcat cgatgacgaa cctcgcgccg ccgcctccca
    32341 tcacgccggc cagatgcccc ggagcgtcgg cgagaatcgc cgagatcggc gcgccggaag
    32401 ggatcgtctc ttccttgacg tagcggatct ccggcacggc cgccgccacg gcaagaacgg
    32461 cggcgggtga cagatcggag ccgcgcggcg cgggcgcatt ctgcaggata atttccacgc
    32521 cggggcaggc cgccgcgatg gcggcgtaga agtcggcgac tgcggccgca tcggtgccga
    32581 tcgccttcgg ggcctggatc atcacctgcc ggagacccag cgactggacc tcccgcacgc
    32641 gcgcaatcac ctcgtcaagc gtcggggcgc tcgcacccgc gatcagcggc aggcgtccac
    32701 cgagttcatc gagcacaatc tggagaagct cgaaacgctc ggtggccgtc aggtcatcaa
    32761 cctcactggc gaagccggga aacaccacgc cctgcacggc ggaatcgcag gcaaaaccga
    32821 cgatccggcg catggccgcg cgatcaatgg cgccatccgc tgcaaacggt gtgcagagca
    32881 ccggccatac gccctggatg gttgtgctct ccaacacgct ctcccgtgcg aatgatccat
    32941 attgtggacc acagtatctt tatatggacc gtagtgtgga tgttggctat ctgtcaagcc
    33001 tgtgcaagga aagcgcccga gggcgcgggg ttgggtagcg atgaagcgca tcgtggtgga
    33061 ttggggcagc agcaacttcc gggcctaccg gttcggagtg gaaggcgcgc tcatcgaacg
    33121 acatcaggcg gaagcgggca tcctcaccgt gcgcgacggc gccttcgagg atgcactcga
    33181 acgcgagatc ggccactggc tcgatcctga gacggagatt ctgctctccg gcatgatcac
    33241 cagccgcaac ggctgggtgg agacgcccta tctcgagccg cccgccagcc tttccggtct
    33301 cgcgacccgg gcagtcgcgc ggccggcgcg caccggcgca cggctcctct tcctgccggg
    33361 cgtctgtaat cgcgcgcccc agccagatgt gatgcgcggc gaggagatcc aggtcttcgg
    33421 ggcggtttcg ccgaccgagg atgcgctcgt cgtcttgcca ggcacgcatt cgaagtgggt
    33481 gagcgtgcgc gctggggcga tcgccggatt ttcgaccttc ctcacgggcg agatgtttgc
    33541 cctgctgtgc cagcattcga tcatcgggcg gctgattccg ccgggtgctg ccggcgcgag
    33601 caacggggcg ttcctcgagg gtgtgaaact ggggttttcg ggcaatccgc gcggactcct
    33661 gaatgatgtc ttcaccgcgc gcgccggagc gctgttgggc gatttcatgc cgaccgaaat
    33721 tgccgagcgt ctatcgggca ttctcatagg gcatgaaatc cgggccgggt tcgagtccgc
    33781 gatcatgccc ggcctgacgc cgcttctcgt cggcgaggcc gcgctgatcg cgcgctatgc
    33841 ggtcgccttc agcgcattgg ggcttgccag tgcgaagggg cccgaccatg ccgccgtgga
    33901 gggtttccgg aaactcggtg cgttgggagc atcccatgcc tgaccatctg acgcggttcg
    33961 aggcggcctt tgccgccatg ccgctcatcg cgatcctccg gggcattcgc ccggaggagg
    34021 ctgtcggcat cggcgaagcg cttgttgccg ccggaatccg catcatagag gtgcccttga
    34081 attcaccgga accgttcgaa agcatcacgc gcctggtccg gcgcttcaag gcggaggcgg
    34141 tgatcggggc tggcaccgtg cgatggccgc atgaggtcga gcgcttgcga gaacttggtg
    34201 ccgggctggt tctgtcaccg catgtcgatg ctgcggtggt acgtgcgagc gtggccgccg
    34261 ggattgtttc gattcccggc attctgaccc cgaccgaagc gttcctcgcg ctcaattccg
    34321 gcgcccatgc gctgaagctg ttcccgatgg aaatgatcgg cactcccggc gcgcgggcga
    34381 tgcgcgcggt actcccgcgc ggcacgcgac ttgttgcggt cggaggggtt ggcgagggca
    34441 acgcagccca gctgctcgac ggggcctgcg acggtctcgg catcgggtct tcgctctaca
    34501 agccaggcga caaggcaagc acggttggcg agcgcgcgcg gcgattcata caggcaacgg
    34561 gctcaggctc cgcgcggaca acatcggggt agcaccctgc ctgatgtctc cgccagtgcc
    34621 cgttggcgtg gcggcgcttc gggagggttc gtttcggcct tgcgaggcag cgtcgggggt
    34681 tggctgtcgt tcccagacgc aatcggcgcg aaatccgttc caggcggggc gcatcacttt
    34741 cggatgtccg gcaggggcgg cgccgacttc acgagcacga tggatacccg gcggttcgcg
    34801 gcaagcttgg gatcgtcggg gaacatcggc tcggtgtcgg ccttgccaac cacgctctcg
    34861 aaccggcccg ccgccacgcc ggaggattgc aggatgtcgc gcacggcgag cgcccggccg
    34921 gtcgagaggc gccagccgtc agcggacttc cggccggacc ggtcggccga tgtgtggcca
    34981 gtgatgcgga tcgggttcgg cagttccatc aggcggggcg cgagcgcggc aagcagcgcg
    35041 cgggtccgat caagcggctg ggtcgagcct tccggaaaca tcgatcgccc gtcctggtcg
    35101 acgatctgca ggtcaagtcc ctcggacgtt tcctcgacga gcacgttcct ggacatttcc
    35161 gagatgtccg gcagatcctg aaatgcctgg cgcagcgagg ccgccgccag cgcgaaggcg
    35221 cgctcgtgcg cgaagtccgt gcgtccgtcc tgacgcttct cggcgcgttt cggcgcggtg
    35281 aattccgagg cctggtcagg cgggatcttt tccaggttgc gcagatgcgg acgcacgggc
    35341 accccctcga tctcgacgat gccggacagg cggctctgct tctgcgatcc gaaggcgtcg
    35401 cgcatggagc ctgcaacgat ctgtagcttc ttctgatcct gcgtcgagaa cgccgtgagc
    35461 atgacgaaaa agctcatcaa caggcccatc aggtcggcaa aggtaacgaa ccagccgtga
    35521 ccgccgccgt gaccgccgcc cttcttgcgc gccacctctg cctccccggg tcaggccgaa
    35581 gcgtcggcga gttcgtgctt gtggtggtcc ggcaggtagg aaatgagcat ttcacggatg
    35641 acggccggat tcttcgaggc gcgcatctgc agcacgccat cgatgatcag cgtgcgcgat
    35701 acctcctcct cttccagttt cacatgcagc ttgtcggcga tgggcaggca aatcaggttg
    35761 ccgaccagag cgccgtagag cgtggccagc agggcgaccg ccatgaaggg ccccagcttg
    35821 gaggggtcct ccatgtttga gaacatctgc accatgccga ccaacgtgcc gaccatgccc
    35881 caggccggcg ccgcatcacc gatggaccgg tagatcttct gcccctcgtc cagatgctgg
    35941 agaaaggtgt cgcggtcctg ctccagggtc cggcggatga agtccgggtc gtagccatcg
    36001 gcgagatacc gcaggccctg cgccagaaac ggatcgtcga ccgactgtcc ttcgagcgcc
    36061 accgggccgg acttgcgcac gatctcggcg attcgggtga tctcctcgat cagcacgcgc
    36121 gggtgctggc tgcgcatggt gaaggcatag cgaatgccca ccggaaagcc gtgtgccatg
    36181 gccgaaagcg ggaagcggat caacgtgccg gcggtgcagc cgccgccgat gacgatgatg
    36241 gcatggacgt cgtagaacat gccgaagttg ccacccatca ggatcagggc ggcaacagtg
    36301 gcgattccgg caaggaaccc gagacccgtg gcaaaatcca tggcggtgtc cgatacaaaa
    36361 ctctaatagc gttactctaa ctagtatctg gacggggaac gttaaggttc ccttgccttt
    36421 tcccgatcgc tacatctttc ggttgtggcg gcgcgcggca ttgccgcagg ccattgcacc
    36481 gacggcggcg aacgactagc tggacgtata gtcttgtcct tgcgcggaga tttgcccgat
    36541 ggtgcccaga cccgtgatgc tcgtgatcct cgacggctgg ggcgaacgcg ccggtcggca
    36601 ggacaacgcg gtcaagctgg caagaacgcc caatttcgac cggctgtggg ccacctgtcc
    36661 gcatgcattc ctgaagacct ccggcctgga tgtcgggctg ccggaaggac agatggggaa
    36721 ttccgaggtc gggcatctca acatcggcgc cggccgggtc gtcatgcagg acctgccgcg
    36781 catcggtgaa gccatccggg acggtagcct tgcgcgcgcg ccggcgctga cggatctgat
    36841 cgtcgccctg aaggcgtccg gtggcgtctg ccacttgctg ggcctggtgt cgccgggcgg
    36901 agtgcattcc catcaggacc atgccctggc gcttgcccgc attctctcgg accacggcat
    36961 cacggtcctg ctgcatgcgc tgaccgacgg ccgcgacacg ccgccgcgct cggccgcgga
    37021 ctacgtgagg agcgtggaaa cgggcctgcc ggcgcgtgcg cgaattgcca cggtgatcgg
    37081 ccgctactac gccatggacc gcgacaatcg ctgggaacgc gtcagcaagg cctacgaggc
    37141 aatggtgtcc ggtggggggc aggcctttgc ctccgcgggc gaggcgatcg ccgccgccta
    37201 tgccgatgac gtcacggacg agttcattct gcccgccagc atcggcggct acaccggcat
    37261 gaaggccggc gatgccctga tctgtttcaa tttccgtgcc gaccgggtgc gggagatctt
    37321 ggccagcctg ctcgatccgg cgttcggcgg gttcgagcgc gcgcggcaac cgcatttggc
    37381 ggcggccgtg ggcatgaccc agtactcgac cgagctggac gcctttcttg cgacgatctt
    37441 cccgccacaa agcctagcga acgtgctcgg tcaggttgtt tccgacgcag gctgcaagca
    37501 gctgcgcatg gcggagaccg agaagtatcc ccacgtcacc tacttcctga atggcgggga
    37561 ggaggcggtc tatcctgggg aggatcgcat catggtgccc tcgcccaagg ttgccaccta
    37621 cgatcttcag cccgagatgt cggcgccgga actgacggac aaggccgtgg cggccatcgc
    37681 gtccggcgtc tatgatctga tcgtgctgaa ctatgccaac cccgatatgg tgggccatac
    37741 cggcagtttg ccggccgcca tcacggcggt cgagacggtc gatgccgggc tcgggcggat
    37801 cgccgaggcg atcggtgccc ggggcggcgc cttgctggtt acggcagacc acggcaattg
    37861 cgaacaaatg cgcgatcttg aaaccggtgg accgcatacc gcccacacca ccaacccggt
    37921 cccggtgttc ctgttgggcg cgcatgcgcc ggcgctgcgc gagggcgggc gtcttgcgga
    37981 tctcgcgccg accctgctcg cgctcatgag gcttgaccag ccgcccgaaa tgaccgggcg
    38041 cagcctgatc atggcttgaa tgcccacgca ttcttcgagt cctcgcgcct gcaagacatg
    38101 ggcaggcccc cggcgggagg ggacgcatcg gggagctgtc gactggccca gtccggaaat
    38161 gcaccgttgt cgcgcgcctg tgcgcctcat cccgcgtccg gcatgggccg ttacgggctt
    38221 ctcgcgcttc tcggccggca agccctgaca ggacttgttg aagtcctggc aaggtgctat
    38281 gcgcctcgcc gaccacgggc cccggccaac gcgccagccc ttgcagactt ctgacggaga
    38341 ggtggcagag tggtcgatcg cgccgcactc gaaatgcgga gtacgtgcaa gcgtaccggg
    38401 ggttcgaatc cctccctctc cgccatttac ttcggaatct agcggcaaga tgatgcaatg
    38461 cgcagaagtg cgttttgaaa acccgctcag gagagatcag gaaacttgaa cgaaccaacc
    38521 gccacgctgt gcggcttgag cggccccggc gaccgtcata tcgctacgcg gccgcttgct
    38581 ggactttgag agaataaggg ctttgatagc ttagctcagc ccaccggttg tcgccgggca
    38641 tgcttcaggt cacgagagcc gcgcgccgaa gcatcacttc ggcgcgacgg acaggttggc
    38701 gaagaacgct tcggttccgt cgtccaccca caggccgatg cgccccttgc gacccggata
    38761 gcggaggtcg gcaaccgtca tcacctcgac gccatcgacg aatgcgcgca acgaactgcc
    38821 atcgatctcc agccgcaggc gatgccagct gtcgatggca actggtgcgg ccttctcgta
    38881 gcggttcgga tgctcctgcc gcagtttccg ccagtagcgg tcgggatagc tgatgtactg
    38941 gatcgcaaac acgttgcgcg gcgcgggcgg cggcggcacg ttgcggctgc cgttggtcat
    39001 gcgcagatag accgcttcga aggtgccgag atcatcggcg atgtggaacg ccagaccaac
    39061 gaaaccacgc acatcgggct ggccgcgccc gttgatccgg gcggccagat ccacctcgat
    39121 ggtgccctca gcgaactcga tggctggcat ggcgaagctc ggaccattac cggcggagcc
    39181 gggcacaagc agcgccttct gggcctcttc ggtcagcgag acggcaacag cgtcacgctc
    39241 cttgaagtgg ccagctgata cctgcaccaa gttggtcgtt agcccttcgg gcgaacgcag
    39301 gggtaaggtc cggggaccgc ctgtctgggc aatagcttgg cgggctgtcg agcatagacc
    39361 gagaaatccg gcggcgagta gagctctacg gttgacccga ggcatgcgcg cttctccaaa
    39421 cattgtcagt cgcgcaggag acgtaggcct gaaattgcaa ttcacaaaat tcatagtatg
    39481 catccatcat gaacaagatc gatttgcgac gggtcgactt aaatcttttg cccgtattcg
    39541 aggtccttat tgaggagcgt cacgtttccc gtgctgccga gcggctcggt cggtcacaat
    39601 cggcgatcag ccacgcgctt gagcggctgc gtgagcagct gaacgacccg ctgctgatcc
    39661 gggttggtgg agttatgacg cccaccgcat acggtctgga gatttttagc gaaatccggc
    39721 ctattctacg gaacatccag cgggtcctat cgacgcgcga aagctttgta cccgatacgt
    39781 cagatcggac ctttcacatc gtcatcccag atttctgggc ggcggccatt gctgctttcg
    39841 tgcgatgcat tcgcatcgaa gcgccaaatg ttgttgttat gtggagccct ccacatgagc
    39901 ggagccttgt cgacctggct aacgagcagg tcgatctcgt cgtcgcgcca agtaagttaa
    39961 aggcacccga aggcatcgag gccgagtcga ttggtgcgct gtcatggggc tgctttctgc
    40021 gtcggggaca tcctgcgctg gaaacgtggg gagcagaagc ctggcaggcc tacccgcata
    40081 tcgtcgtgaa cgttggcgaa aggtttccct cgcccgtcca agtcgccagc catccagggg
    40141 ggttgttgcg gcgggtcgga gcttatgttc cgacattcgc cgcagtgccg cctctgcttg
    40201 cagagtctga catgatagca accttacccc gccttgctct ggtcgaaggc gcaagggtct
    40261 atggccttca ggacaggctg cccccattcc cgattgcgcc gattgatgcc agcctcttct
    40321 ggagcgctcg acgcacgagc gacgctgcga cacgctggtt catcgagcgc cttcgaccgg
    40381 cgctcgtgga gcgtatcgtc taggcgcgcc ggcacttcgt tcgtgacctg cctccggttt
    40441 tctggaccgt tcatttctgg aaaattccag tttagcagca cgggctgcgg atggagtttt
    40501 cgacatgaag aagtcgaagt tcacagaggc gaagatcgcc ttcatcttgc gtcaggctga
    40561 ggaggaacgc cggtccctga ggtctgtcgg aaggccggga tcagtgatgc gaccttctgt
    40621 aactggcgca agaaacacgc cgggctgatg ccctcggaga tgaagcggtt gcgccagctc
    40681 gaggaagaga atgctcggct gaagcggatt gtggcggacc attcgctcga caagaagatg
    40741 gtgcaggacg tcatcaagca aaatctctgg ggcctgcccg ccagcggtcg atggtcgatc
    40801 atgtgcgggc gacctggcag gtgagcatcc gtcgtgcttg ccgcgccttg cccgtcgagc
    40861 gttcgaccta ccattaccgc tcccgacgga ccgggcaggc ccatctcatc aaacggatca
    40921 aggaaatcgc cgagacgcga atgcgctacg gctacaggcg gattccggtg ttgctcaggc
    40981 gcgagggccg gagtgtcaat ccgaagcgga tctatcgtct ttacaaggag ttggggctcc
    41041 agctccgcaa caagacacca aagcgtcggg tcaaggcgaa gctgcgcgag gatcggcgtc
    41101 cagccacacg gacgaacgag atatgggcga tggacttcgt gcatgatcaa ctggcgacgg
    41161 ggcgcaagct ccgcgtgctc actattgtcg acaccttttc gcgcttctcg ccagcgctgg
    41221 atgtccgctt caactttcgg ggggcggatg tcgttgaagt cctcgagcag gttggccggc
    41281 agcatggctt cccgagcgcg atccgggtcg accaaggcac ggagttcgtc tcccgcgatc
    41341 tcgatctctg ggcctatcag cgtggtgtca cgctcgactt ctcccgaccg ggaaagccga
    41401 ccgacaacag ctacatcgag tcgttcaacg gcaagttccg ggccgaatgc ctcaacgcgc
    41461 actggttcat gagccttccc gacgcccggg agaaagcaaa ggcttggcga agggactata
    41521 ccgagcgaca acctcacagc gcgatcggga acaagcctcc gataacgctc gtcaatcggt
    41581 catcggcatg cagcccgcga tgagcagatt accccctgga agcggccggc aagccggtcc
    41641 aacatcgggg gcaggcccgc ttgtgatgga cccaactgac cgatggcggg aattcagggg
    41701 cacacgtcaa catccccaaa ctcagttgcg ttttcaggct cgaaattgat cgacttcgcc
    41761 gctcttaaat tttggtctgc atctgtgaca gtcggggtag atctaagggc gtgcgcacga
    41821 tgaggctgtt gacgacccgt cgccaaacat ccccctacaa aaccacctga aatatcggcc
    41881 attcagcttg ggttcgaatg ccctgccccg gcatcccccg attgcgctgg tgctggctct
    41941 ttaggttccg gcttgggcgc gcgagcacgc cccatccagg ctttgacgac ctcccgcgcc
    42001 gactggaacg cgacgtagag accaggaatc agcaggatgc cgacaagagc cgcagccaac
    42061 atgccgccga atacggccgt gccgacggcg cgctgcgtcg ccgccccggc gccgttggct
    42121 ataaccagcg gaagcagccc gaggatgaag gcaaaggagg tcatcatcac ggcgcgaaat
    42181 cgctggttcg ccgcatcgat cgccgagtcc acgatcccct tgccgtgggc gcgattctcc
    42241 gcagcgaatt cgatgatcag aatggcgttc tttgcggcca gcgcaatcag gataacgacg
    42301 ccaatctggg caaagatgtt gttgtcgagg cccgccagct tgagcgccat catggcgccg
    42361 aacaggccgg cgacgaccga cagcaagacc gcgaccggaa ttgtccagct ctcgtaaagc
    42421 ccgactagac acagataggc aaagagcatc gccagaatga gaacgatggt tgtttgcccg
    42481 cccgcttgtt tttcctggaa tgccgtgccc gtccactcca gtttgaagcc gcgcggcagc
    42541 gatgctttcg ccacatcttc catcgcggcg atggcctcgc tggaggaatg ccccggcgag
    42601 ggtccgccgt tcaacatcgc ggcgcgctgg ttgttatagc gcataatgat ggcggggccg
    42661 gtgaccagtc gtatcgacac gacgctggtc agcggcacaa gatcgccatt cgacgttgag
    42721 atgcgaatgc gctcgatgtc ggaagcggac atgcgcgccg cagcctccgc ctgcacgttg
    42781 acctgccacg tccgtccaaa caggttgaag tcgttcacat aggttccgcc aagcaccgtc
    42841 tgcatggttg agaagatgct ggcgacgctg atgccgaggg tctgcgcttt ctcacggtcg
    42901 acgatgactt tcagctgcgg cgtatcggca ttgaaggtgg tgaagacgcc ggcgaggcgc
    42961 ttgtcctgat tggcgctgat catcgtgctg cgcgccacct gcgccagcag gaccggatcg
    43021 gccccttcaa gacttgtcac caacgcctga aagcccgagc ccgtgcccag tccctggatc
    43081 ggcggcaggt tgaaggcgaa ggcgaaggcg tcggcgcgcg ccgcaaactc ccggttgaga
    43141 gaggcgatca cgctgaaggc ggactgcgac gcctccttgc gctcgtcaaa cggcttcatc
    43201 gtggcgacga agaaggctcg gttcggcagg atcacgccat cgagcagcga aaacccgttg
    43261 accatgaaca cgtcctggat ggcctcgcgc ttggcgatga cctgccggat cgcttcgccg
    43321 acgctggcgg ttctttccgt cgaagcgact gccggcagtt ggacctcgcc catgaaggcg
    43381 ccctgatcct ccagcggaag gaagcccgcg ggcacggtct tggcgagcca gcctgttccg
    43441 gcggcgaaga atgccagcag accgagcgtc aaaacggcct ttcgggccaa tggcgcgata
    43501 agccccatga acccgccccg catcctgtcg acgcccgtat tgaacatcct gaacaggcct
    43561 ttgggcgcgg ccttgtgcct gaggatgacg gcgcacatcg cgggcgaaaa agtcagcgcg
    43621 ttgaccgctg aaatcaccat ggacgcagag accgccacgg cgaactgcat gtagagctgg
    43681 ccggagatgc cgggcatgaa ggcggtgggc acaaacaccg aaagcaacac gagcgtgatc
    43741 gcgatgatcg gcgccgtgat ctccttcatc gcagcctcgg tcgcttcggc gacggtcatc
    43801 tccggatgcg actccagctt gtgctcgacc gcctccacca cgacgatcgc atcgtcgacg
    43861 acgatgccaa tcgccagcac cagcgccagc agggaaacgc tgttgagcga gaaccccatc
    43921 gccagcatca cggccagcgt tccgaccagc gccacaggca cggccaggat cgggatgatc
    43981 gtcgcgcgga aactgccgag gaagatcagc acgacgatag cgaccagcac gaacgcctcg
    44041 cccagcgtgt gcatcacgct ttccatcatc ttttcgacga agtaggtcga gtcgtacatg
    44101 atcttgattt cgacatcgtc gggcagacgc tgcctgaggc cctcgacgac agtcttgatg
    44161 cgctcggcgg tcgccacggc gttggcgccg ggcgcctgat agatgccaag gccggcggcc
    44221 ggcctgccgt tgaacgaagg ctgcgaatcc tcggtccggg cgccaagctc cacccgcgcg
    44281 atatccttga tggtcacccg tgcgccgtcg ggattgaccc ggacaatgac gttctcgaat
    44341 tcctctacgg ttctaagccg accgtccgcg ctgaggttga cctgaaagtt cgtgccgggc
    44401 accagcggat tggcgccgat ccgccccacg gccgcctgca cgttctgggc gcgcagcgcc
    44461 tggacgactt cgcccgccgt aatgttcagg ctggcgagac ggttgacgtc cagccatacc
    44521 cgcatcgaat aatcgcgcgg accgaaggcg aaggcctgtc ccacgccctc gatgcgcgcc
    44581 agcggatcga cgacgttgag cttggtgtag ttcgacagga acagggcgtc ccgcgagccc
    44641 ttcggcgaat agacagtgac aacctgcaac aggctggtcg atttcttggt gaccgtcacg
    44701 ccgagcgcct gaacctcgga cggcaacagc gcggttacct gattgacgcg gtttgaaacg
    44761 ttgaccgtgt tcatgtcggg attggagccg accgcgaagc tcacgttgag cgtgtagacg
    44821 ccgtccgatc ctgagacgga ctgcatgtag atcatcttgt ccacgccgtt gatcgcaggc
    44881 tcgatgacct gcgcgacgga ttgttcgagc gcggctgcgt cggcgcccgg atagaccgcc
    44941 gtcaccgaca cctgcgaagg cacgatgtcg ggaaactgtg cgacgggcat agcgcgatag
    45001 gcgatcaggc cggcgatggt gatgatcagc gaaatgacga cggcgaggcg cggtcgcctg
    45061 atgaaaatgc tcgagatcat cgcctacttc ccggcttcgg cgcgctgggc attcaccttc
    45121 atgccgggac gaacgcgttg catgccctga acgatgaccg tttcgccttc cttcaggccg
    45181 tcggtgatga cagcaatgtc tccgctcgac tggccgacct tgacgtaacg aactgacacc
    45241 atgccctggc cgtcgaccac gagcgcgaaa gtgccggtct ggtccgccgc cagcgctacc
    45301 tgcggcgcaa cgacctgctt gccctcggcg ggttgctcga tcagcacgcg cacggtctgg
    45361 ccatgggtca gctgcccgcc cggattggcg aatgtggcgc gaagaacctg cccgtccgtt
    45421 cggctgtcga caacattgtc gatatagtcg attgcgcccg tctgtgagta cagcgaccca
    45481 tcggcgagtt tcagccggac aatcggctcc ttcgtcgcct tgcgcttccg gtactccaga
    45541 acttcacgct gactgaccga aaatacgacc cgcatctgat cctctttcac gatcacggcg
    45601 agcggcccgt tcgatggcga cacgatattg ccctcggtaa tcgccgttcg accaatccgc
    45661 ccgtcgatcg gcgcacgaat atccgtataa ctcaacgtga tcttcgcgtc ggaaagggcg
    45721 gcctcggcga tccgcacatt ggcgtccgcc ttggcggact ccgccttgcg ctgatccacg
    45781 gtcgccgcgg ttccggcgtt ggtgcgcagc agttcttgcg cgcggccaag attgagcgct
    45841 gcgttctggg cgtccgcctt tgcgctggcc agctgagcct ctttcgcgtc gacagccgcc
    45901 ttgaatgggt ccggctcgat cagatagagg ggctggccgg ccttgacctt ttcgccatcc
    45961 ctgaatttcg gcgcttccag aagccctgaa acgcgcgccc ggatttcgac gcgctcgacg
    46021 gcttcaatct tgccaataaa ttcgttgtcg cgcccgattt cgcgaagctc ggcggccttc
    46081 acgatcacgg tcggtacagg cgccgactgc tgcgcctgcg cggtcgagag caacgccatt
    46141 gacgccattg caaccagaag tttgagcctc atgaacgcct tcccaattct cgcaacgagc
    46201 ctccctactg caggcctatc tcatacgtat tacagttgag gcacggcgca aggctgggca
    46261 cgtccaaaag ccatatcatt gaagcatttg ttctgattca tttccttggt ctgagcggga
    46321 tgaatcggat ggatcagcgc ggcaggcttg ggccgagagg tcacggatag agtgttctca
    46381 agaccatggt ttgccttacg gcacgcgccg tccctgtgcg tcgatcagga gttcaccatc
    46441 ctccttggcg agaggtccgc gaggtaggcg gtccagcaga tcaaggacga cttcactggg
    46501 gcggcagagc ttgacgccct tgggggtgca gacgattggg cggttaacga gaaccgggtg
    46561 ctccagcatc gcggcaagaa tggtttcatc gctgattccc ggctcaagca agccaagctc
    46621 ttcggccggt gatttcgtag cgcgttgcgc ggcgtgagat tggccgcggc aaagagcgcc
    46681 agcagctgtg gccgcgtcca acccgtcttg agatactcga cgacgtccgg ctcgtatccc
    46741 gccgctttga tgatggcgag cacgttacgc gaggttccgc aggcgggatt gtgatggatg
    46801 acgaccggga acatcagtgc tttccttcca ccatgcgggt tctgaccgca ctgccggcct
    46861 cgtaccaatc tttcgagcgg ttcacgatgg cgacgaccga gagcatgacc ggcacttcga
    46921 tcaacacgcc gacgacggtt gcgagcgccg cgccggactg aaagccgaaa aggctgatcg
    46981 ccgcggcgac cgcgagttcg aagaagttgc tcgcgccgat cagcgccgag ggaccggcca
    47041 cgcaatgggc ttcgcctgtc cagcggttga gcagataggc aaggcctgaa ttgaaataga
    47101 cctgaatggt gatgggcaca gccagcatcg cgatgaccag cggctgcgcg atgatctgcc
    47161 cgccctgaaa agcaaagagc agcaccagcg ttgccagcag cgcaacaagc gacagcggct
    47221 ggagaacggc gaggaagcgt gcaagtgcag gctcgccacc ggttgccagc atctgccggc
    47281 gcagaacctg cgcaatgatg accggcacga caatgtagag cacgaccgag aggaccagcg
    47341 tgttccacgg cacggtgatc gccgaaagcc caagcagcag cccgaccaat ggcgcgaatg
    47401 ccagcaccat gatgacatcg ttcaccgcga cctgactgag ggtgaagtgt ggctcgccct
    47461 tgcagaggtt cgaccagacg aacaccatgg ccgtgcaggg cgcggcggcc agcaggatca
    47521 gcccggcgat ataggaattg atctggtccg ccggcagata ggggcggaat agccaagaga
    47581 tgaaaatcca ccctaacagc gccatcgaga atggcttcac cgcccagttg atgaatactg
    47641 tgacgccaat accgcgccaa tgcgctttga caccggagag cgcggagaag tcgatcttca
    47701 ccagcatcgg gatgatcatc aaccagacga gcagggcaac gggaaagttc acctgggcga
    47761 tttccatcgc gccgatggcc tgaacgggcc ccggcaggag atgtcccagt gtgatgccca
    47821 cgacgatgca aaggacgacc cacaaggtca ggtagcgctc aaatgtcgac atatccggtt
    47881 tctcgggtgt ggcgcagaac tcaggcgttg agccaaaccg ccagatcttc ttccttcggc
    47941 agcccgcccg cgtgaaccac cttaccatcg ataacgatgc caggggtcga ggcgatgccg
    48001 taaccggcaa tcgccgcata gtccgtcacc ttctcgacgt tgacatcgat gccaaggcgc
    48061 gcggcttcgt cgcgcaccat ctgcaccgta gcctcgcatc gcttgcagcc aggacccaat
    48121 accttgacgg ttttcactgt ttgtctcctt caggcgaaca cgaaattgaa gaggaagccg
    48181 acagcaagaa tgccgcttgc cacaaccgcg acaaacaccg cgatgagttt aagcgtcagg
    48241 accttcctca ggatgaccat ttcgggaacc gagagcgcga tcacgctcat catgaaggcg
    48301 agcacggtgc cgagcgccgc cccttttcca agcagcgcct cgacgactgg gatgatcccg
    48361 gcggcgttgg aatacatcgg tatgccgatc agaactgccg ccggaacgga ccaccacgta
    48421 tcccgcccca tgattgagac gagcagttca gagggcacat atccatggat gaaggcgcct
    48481 gcggcgatac cggcgatgat ccagagccag actttaccga caatctcctt caccgcctcg
    48541 atcccagcct tgatgcggtc gacagcggtg agtcgattgg ccggcaggtc ggcttggccg
    48601 gcgcgcaggt tgcgcaccca gtcttccagc catccctcca gcttgaggcg tccgatgatc
    48661 cagccggata cgatggctac ccccaatcca aacgcgagat aggtcaatgc caccttccag
    48721 ccaagcagcg cgaagagcag ccccagcgcg acctcattga tcattggcgc ggcaatcagg
    48781 aaggagaagg tcacgcccag tggcacaccg gctgacacaa agccgacaaa cagcggcacc
    48841 gccgaacagg agcagaaggg cgtgaagata ccgagaaccg ccgcagcaat attgcccacg
    48901 ccttcagccc tgccggcaag cagagctcgg gttcgttccg gagagaaaaa gctccggata
    48961 acccccatgc cgaacaccac gagggtcagc agcatcaaca ccttcggggt gtcgtagagg
    49021 aagaacttca ccgcctcata ggcgtggctc cccttggcca acgggaacaa ccccaccgcc
    49081 ccttccgaga agggcgcaag ctggccatag accgcaaacc agatcacaac aaaggcggca
    49141 acgccaaggc accagatcca ggtcggccga tgccgggatg cgccacgaag tgcggatgtc
    49201 atgcggcgct cctctggcag cttgatgttc cctccgacag cggcggatcg tctgatgcgg
    49261 caagaacggc atcgaccacg gcctgcaaga tcggatcatc aagggtgcga cggcggtaac
    49321 gcatccattg cgcatcgcgc cggtccgaaa tcagcccgac ctccttgagg cacgccatat
    49381 ggcgcgacat gctcgactgg ctcacgccaa gtctgtccat gagttcgcac aggcaatgct
    49441 catcgcctcc cgcaagcaag cgcagggccg agcggcgggc cggatgggac agtgcagata
    49501 agagcgtatc gagttgtggc atgcagggac taatgcaatg aaatgcatat gctgtcaata
    49561 gcatatgcaa gagacgacat atcctgttct tccgtgaaac ggctacgccg catggtccat
    49621 ctccttggtg atggacccaa cttaccgatg gcgggaattc aggggagcac gtaacggtca
    49681 accatcattc ttttgggcaa attctttcaa gaacctgcaa ttgcgtcgcc taagccacaa
    49741 cagtgcccga gctaaggaaa atgccgttcg cattgcggaa ctggcttgcc ctcagcagga
    49801 agtcttggga gaaattgccc atgtcccagt tcgagatgca gatccgcgcc atcgcgcgtc
    49861 aatatgagat cctatcaaag ggggacggcc agaaggcgaa cgtcgtagct gaggcgatct
    49921 tgcgctgtgt tgagaacgga tattggcaac caaacgacaa gatgcccgcg gaactgtcgc
    49981 tggcgaaagc tttgccggtc tctttgggca ccatacaggc ggcctatcgg atgctgacgg
    50041 ccgagggggt tgtcgcgcgc cgccgcaagg acggtacccg cctcacctcg caagtcgatg
    50101 tcaattcctt gaactggcac ctgcgatttt ccgatatgga aacgggcgca tcacttacat
    50161 gtcggagcac tgtgttttcg atcaccaaga cccacgattc cgggggctgg agcaaatttc
    50221 tccctgatgt tgcagagttc acctgcatcg caagacgctg gtccatcggc gaacgctttc
    50281 aggtgctcag caggctctat atatcagccg agttttacga gcccttggcc gagttgggta
    50341 tgaatgccct caatgatatc agtttgcgtg cggtcttcag cgatacactg aggatcccga
    50401 cgctgcgagc gacccatagc atctcgttta ggccgcccac cgaggaggag gtcgctgttc
    50461 tcgcggtcat gaccgacagg cccctcatgg tgctggaggc ctgcggctat acctaccggg
    50521 atcaaccgtt ctcctatcag gtgttttcca tgccgacgga gttgcgcaat gttcgcctga
    50581 tggtctgagc acgtccgatg gcaagccgca ttcaacggat cacatagatc gggctggacc
    50641 aggcaacgtg cccatcctcg gtgcgcaccc gcacccagac tggcgtatcc cctgcatgct
    50701 tcagccaaac gcgatgggaa acagtcattt ccgttgcgtc cagtcggtcg ggcaatcgtt
    50761 gcaccgaaat ggcgcggtca agaccaccgg catccagcac gcacgcctcg accccgagct
    50821 gtttcaggtc gaaagatccg gtcaccacgt tggtggcaat gttcgccgcg ccctgaagcc
    50881 tgtctagtgc tgcatcgaag ccaacaaaat tgccggtggt aaccgtttca aagcggatcc
    50941 tgccagggcc atcgtgaaca agcggccgct ccgggttcca ccagttgatg ggtgtgatct
    51001 gagccacggc tgcatcggaa aatgtcaccg tgccggtcca tttggtctgg cgcccgcgtc
    51061 cgcgatactc cgccccgttg cagaggatgc gaagccgatt gcccagatcg tcacgcgtgt
    51121 agcttcgaaa cgtcgccgcg atgtcacttg cggtgaacag gtcgatcgag aggattggcg
    51181 aaccagccga gacgctgatg ttcaccgttg ctgcctctgc cgacgttcgc gcaatctcgc
    51241 ccatctctgc ccgactgctg gcgccatgcg ggccggcgcc agtggcagga tcagtatgaa
    51301 agatctcggc cggcgcatcg aaagcaaggt gaaggtccag gtgcagccgg gagccggtgg
    51361 tgccgtagtg gtttctcctg cgaatggcat tgaacaggcc atccctgtcg agcgcggcgg
    51421 cccggaagca ggtcagccct cccagggcgc caaaacttgc cgcgccggga taactggcgc
    51481 cggggcggcc cttgtgcccg tcgctattgc aaacgacgcc gacgcgatac ccgaggcgga
    51541 aactgtcatg gagcatccat tcgaaagtgc cccagtcgga atgaagctcg acagctaccc
    51601 ggagccgcgg gtcatgagcc gccgcaaggt tcgcagggcg gccaccgaca tgggcgaaca
    51661 gcacgcagtc ctctttcgca agagcggcat agagcgccgg cagatcattg gcgtctgtct
    51721 gcaggcggtg cctgttctcg atcatggcgt gcgacgatcg aataatcggc ctgtcctcat
    51781 cccggaaaaa gacgttgtga tcgcccccaa cagcggtgtt ggctgaccat tcgtacccgg
    51841 gaaaggtcac gaaatggcca tcgtcgttga acgcgcgcgt cgtggtgttg atctcgtccc
    51901 agaaggcatc cttgatctgg aagtcattgg cttgatgcgc acaggcatca acaaaggcga
    51961 tgtcccgggc gaaggtgaag tattcctcaa tggaattgat cccgaccgtt tcaccgctct
    52021 gcccgtgcaa gtctgcccag aaggtctgat cggtatcgcc gatcgacaac ggcacgccct
    52081 cggcaagctt cgcgccagcg gcgtcgaaga gcgaaaatcg ataggtgcct ggcacatcaa
    52141 tccgctgttc gatccgatga gatcgagcgc ccggcctcca gtcgatccgc actgccggga
    52201 tcgatgcggg ccccgttgac tgaagaacca acggcccttt gacagcatcg gaagcattgc
    52261 cccatcgatc ttcgccaacg atgcccacgg caatcgtggc cccaaccctc cgggcagtgg
    52321 gattgatcac tttccagcgc acaggcgggc ctggcagcac gggaatggtg acccggtctt
    52381 cgaggggaac gaaaaagccc gtggcgcagg gatcgaccga aacgcgaagt tcaaacgcgc
    52441 tctcgcagaa cgtttgcatt cgaaatcccg gcgaaccctg cgaacgatcg ccaaatgtga
    52501 cgtagatctc atctcccggc ttgagcgcgc cgcccacgac ctcgaagcgc acagttttat
    52561 cccagggccg aagcccgtga ttttcgcaat ggaccgcaat gcgcacgccg gtcgagcttt
    52621 tcgcactgat gtaatttgcg gccccggggt catgggactg cagtgcaccg ccgtcatagg
    52681 tgaaccgcag ggatacccga aagccgccgg tgtcatcgaa gccgaaacgc ccgacaatga
    52741 gcgtcaggct gatctcttgc cagcttctga cctcgcaagg ggcgctgctg gcgtttgtca
    52801 ctcgtgcata gtccgacgtg gcgcggccgg gttgcaccac cggccagacg tcgcccatca
    52861 gatcgtctga gatcatcgcg gccttcctcg gctcccttga atttttccgc agccccgaaa
    52921 tacggattgc aatgattaac tacatagtta ttaattgccc tgcaagcccg gttgaggagt
    52981 cggaatgcaa gctcttgtcg cgaccgacat ccggaaggcc ttcggttccc tcgaggtgct
    53041 gcggggggtt tcgctgcgag ccaacaaggg tgacgtgatt gcgatcattg gcggctccgg
    53101 ttccggcaag agcacgttcc tgcgttgcct taatcttctc gagacgccgg atgcgggcag
    53161 cctatggctg aacggcctcg ctctggattt cagccgaagc gcgccatcaa acggtgtcgc
    53221 gccgaaggcc aaggcggagc tgcgtcgccg gcttggcatg gtcttccaga gcttcaacct
    53281 ctggacccat atgagtgccc tcgacaacat catcgctggc cccatccacg tcctcggcca
    53341 tgaaaagaag gaggctcggg cggcagggct cgaattgctc gacagggtag gcctggctga
    53401 caaggccgga gcatttccgg cgcaactctc aggcggacag caacaacgcg tggcgatcgc
    53461 acgggcgctg gccatgcggc ccgaggccct tctgttcgac gagccgacat cggcactcga
    53521 ccccgagctt gtcggagaag tgctgaaagt cattcaaggc cttgccgccg aaggccgcac
    53581 gatgatcctt gtaacccacg agatggcctt cgcccgcgac gtggcaacgc atgtgatgtt
    53641 cttccacgac gggcgtgtcg aggaggaagg caaaccaagc ctgatcttca gcgatccggg
    53701 gagcgagcgc tgtcgttcgt tcctcaagaa gttcctgtaa acggaggtga gacatgaaaa
    53761 ccggactgaa acacctggtt cttgcaattc ccctgtgctt catgggcacg gctcatgcgc
    53821 agacgccagt ggtcaagatc gccacggaag gcgcttatgc gccctacaac ttcaaggatg
    53881 ccagtggaaa gctggtcggc ttcgagatcg acctcgccgc cgaactttgc aagcgcgcca
    53941 ccttgacgtg cgaagtgcat gaacaggcat gggacggcat cctgccgagc ctcatggcga
    54001 acaagtatga cgcggtcatg gctgcgatga atatcaccga caagcgcgag gccgtgatca
    54061 gcttcagccg cccctacgtg aagacgccga tccgcttcgt cacaaccaag aaagggacgg
    54121 gcgcggatta cgtttcgtcc gtcactgcga tcaccctaga agatgttgat gcggccgagg
    54181 tcgccgaaat cggcaaattg gccaagttgc tggccggcaa gaccatcggc gtgcaggcag
    54241 caactgcgca tgaagccttc gtcaaggagt atatgaaggg tgcgaccatc aagtcctacg
    54301 acaagatgga caacatgatc ctcgatctca cggcgggacg gatcgacgtg ggctacacct
    54361 cagtcacttt ccttgcgcca attctcaaga agacgcctga tctggttgaa ttcggcccgc
    54421 cgatgacggg tggtgctcta ggtcgcggcg tggctgtcgg tgtgcggaaa gccgacaccg
    54481 aactgcaaaa gaagttcaca acagcgatcg aggccatgct cgcggatggt tcgctctctg
    54541 cgctcgctgt gaaatggttc ggactcgaca tttcggccaa gtgacacggg gaggccccgg
    54601 aggggaacct ggatggatct tggcattctg acctggggtg ataccggttg gggcgatgaa
    54661 ctggcccgtg gcgtgctgac gaccgtagcg atcgccacgg cggcctatct cgtcggcacg
    54721 ctccttggca ttctcctcgc gacgatgaaa ctctcgcgct cgccggcgct gaatgtgccg
    54781 gcgaccatct acaccacaac aatgcgaggc atgccggaga tgctggtgat ctacctcgtc
    54841 ttcttcggcg gcggcgccct gagcacctgg attgcgtcca gcgtcctcgg cattcgcgga
    54901 gtgtttgatc taccgtcttt cgccgtgggc gtcgtctgca tcgcgctgtc cgtcgccgcc
    54961 tatgcctgtg aagtgatccg gggggcggtg ctggcggttc cgggtggcca gatcgaggcg
    55021 gcagaagcgg tcggcatgtc gcggcgacaa cgaaaccgcc tgatcatcgc cccgcaagcc
    55081 ttcaggatcg cgctgcccgg tatgaccaat gtctggcaat ttgccctgaa ggacacggca
    55141 cttgtgtcga tcatcggcgt aaccgaagtc atgcgcacgg cctcaatcgg ctcggatgca
    55201 accaagcagc ccttcacctt ctacttcgtc gctctgatga ttttcatcac aatcgcctac
    55261 ctgtcacagc gcctgttcgt tcggctccaa cattccgctg atcggggcgt acgatggggg
    55321 gcctagattt cctcaccttt ttcggcgatg ccctgtcgca actgtctgcc ggcattccgc
    55381 ggacaatggc gctcgccggc acatcgctcg tcgtcgggtt cgttttggca ataggtgtgg
    55441 cagggggatt actctcgcgg gaagtcgtgc cacggagcat agccaatggc tatgtctata
    55501 tcttccggtc gagcccactg ctcgtgcaga tcttcttgat ctattatggt tcgggacagt
    55561 ttcgcggctt ctttgatgcg attggcctct ggggcctgtt tcgcgaggcg tggttctgcg
    55621 ccattctcgc gcttgcgttg aacacctgtg cctaccaggc cgtgctgatc gccggtggat
    55681 tgcgggctgt accctggggg cagatcgaag cggcacaagc ggtcgggatg tcgcgataca
    55741 gggtttttgt aacggtcgcc tttccccaag cgcttcgtca cgccctgccg gcttattcta
    55801 acgaggtggt ccttatgatc aaggccacgg cgctggcatc aacgatcacc attctcgaaa
    55861 tcaccggcct tgccaaacag atggcatccg aaacctatcg ccctttcgag gtctttgtcg
    55921 cggccggcct catttacatc agcatcaacg gcgccatcgt gaccgggttc atgcaactcg
    55981 aacgatactt gcgccagaat gcgacgctga ggacttgagc gcgagatcaa ccggtcagat
    56041 acccggggaa gggccgttcc cgaaaaggac gttcatgcca cctcggcccg cagcagcatg
    56101 ccgaagaggt cgcgtgggtc gtccggatcg gccaaaaggt ccagctcgtc gaacagcccc
    56161 gtacggccga tctcgccggc cagccagcac agcgccgaga aatcctcgat cgcgaagccg
    56221 accgagtcga acagcgtgat ctggctgggg tcgcgccggc cccgctcctc gccggcgatg
    56281 acccgccaaa gctcggtgac cgggtgatcg gcagccagct gctggatctc gccttcgatc
    56341 cgcgtttgcg gcggatactc aacgaagatg tcggaccgct tgaggatatc ggcatgcagc
    56401 tcggtcttgc ccgggcagtc gccgccgacg gcgttgatgt ggacgccggc cccgaccatg
    56461 ttgtcggtca ggatcgtcgc gttcgccctg tcggccgtga cggtcgtgat gatctcggcg
    56521 cccataaccg cggcctcggc cgagcggcag atctcgatcg taaagccaaa gcgggcgagg
    56581 ttgcgggcgc agcggctgct tgccgccgga tcgatgtcga acaggcgcag ccgatcgatg
    56641 ccgagcagcg ccttgaaggc gagcgcctgg aactccgact gggcgccatt gccgatcagc
    56701 gccatggtcc tcgcgccagg tggcgccaga tgccgcgccg caacggctga cgtcgccgcc
    56761 gtccgcagcg cggtgaggat cgtcatctcc gtgaggagca acgggtagcc ggtgtcgaca
    56821 tcggccagta caccgaaggc cgtcacggtc tggcgcccgt cccgcgtgtt cttcggatgg
    56881 ccgttgacgt atttgaacgc gtattgtccg ccatcgctgg tgggcatcag ttcgatgacg
    56941 ccatccgtgc tgtggctcgc caggcggggc gtcttgtcga agtgctccca gcgccggaag
    57001 tcggcctcga tgcgcgcggc cagttcggtc agcacctgct cgatgccgcg cttcaggatg
    57061 agcttcatca tgccgtcgac gctgacgaag cggacaatgt taagtttgcg attgtccatc
    57121 tcagaagctc cgggtcggac gatccagcac agagcgcccc atcaggctgg cggccagatc
    57181 aaccatcagc cgcgcggtac ggccacgctc gtcgaggaag ggattgagtt cgaccagatc
    57241 cagacttgtg acgcggccgc agtcatgcag catctccatg atgagatgcg cctcacggaa
    57301 ggtcgccccg cccggaaccg tggtaccgac acccggcgca atctccggat cgagaaaatc
    57361 gacatcgagg ctgacatgga ggcagccccc ctccctgacg acctcctcga gaaaatcccg
    57421 caacagggtc gccacgccgt tctcgtcgat cgcccgcatg tcatgcacgc ggatgccggt
    57481 ctgggcgagc aggaggcgtt cggccggatc aacgctgcgc agccccatca tgcagatccg
    57541 ggccgggtcc agcggcgtct ctagcggcgg gaaatgcccc gcgaagccgg gcaaccccag
    57601 cgcataggcc atggggacgc catggagatt gccgctggtc gtcgtgtcga gcgtatggag
    57661 gtccggatgg gcatcgagcc agagcacgaa cagcttgcgc ccgctctcgg cggcgtgccg
    57721 ggcatggccg gacagggtgc cggccgcgag gctgtgatcc ccgccaagca cgatcggcag
    57781 accctcgcga tgggctgtca ggaccgcgtc ggccagcatc gccgtccagc tcgcgatttc
    57841 cggcagggac cggatcgcgg cattcggatg cggggcgccg ggccggatct gcggcatcag
    57901 attgccgcga tcgcgcaccg catagccgag gctctcgaga gccggcccta tgccggcggc
    57961 acgaaaggca ctggggccca tgtcacagcc gagccggccg gttccatcct gaaccggagc
    58021 gccaatcaaa atgcaggtct tgtcggtcat cacgcagcct cccgccgagg aaggctaggt
    58081 gacgctgatg gcggaatgaa gcaggaaaat tgccttattg ttcagcttaa cttatcattt
    58141 tgaaacacaa ttatgacgat ttggatgatc atggacgact tggaccaacg gctgattacc
    58201 ctgctccgcc atgacggccg gcgcagcgtc tccgaccttg cgctggcctt gaaggtatcg
    58261 cgtgcaacgg tccgggcccg gatgcagcgg ctcgcgcaaa gcggcgaggt gatcggctac
    58321 acggtggttt tgaaagcgga tgtcgtctcg gcgcctgtcc gcggcattac actgatcgag
    58381 gtcgaggggc gcaccaagga cagggtcgtc aatgcgctca gcggttttgc ggagatcacg
    58441 gcgatccaca ccaccagcgg caaatgggac ctggtcgccg agttgagcac cgacagcttg
    58501 accgcgctcg acggcctgct tcaccgcatc cggctgatcg cggggatcgg tgcatcagaa
    58561 acgcatctgc ttctggcaac cccccgcagc agtcaggcgc gcctctccag tggttagggt
    58621 aagcaggcga ctgcttgaaa gcgtgagtgg caggagtgcc agctttcttg gcttctgatc
    58681 tcgacagatc gcttcaagca atgcgaaccg gtctgttgaa gtgcgaaccc gttcttacga
    58741 ggttcggcca gaaaaagtga catgttccga cttccaggat cagaagcgat gtggaaattg
    58801 gtgcctaaat ccccgacctt ttcggccctc tgagaacgcg tcttcggaac catcgccgct
    58861 aagtcccgga cacttcggga acacacgcct caattaaatt attgatgtaa ttgaataaga
    58921 tagaaggcat ccgacatcga tatcaaacca cgctcgtgtg gttcgacagc gcttcttcca
    58981 gaagactccg acaaaaactg cagacatcga ctctgcgtag cagatgcata cgtcctgacc
    59041 aatatgttgc tcagaacggc gtttttactg cttctgtagg ctgtacgtgt ataaatccaa
    59101 ttggatctac tgatccgaag gcggcttggg tcgctcgatg atttcgtgat gaagtacgat
    59161 ctgagccggc gtgttttgca tcgacagcgc ctggacgata gcagcgccga aagcgtcgta
    59221 ggggatgcgt accgaactga gttccggtgc cagccattca ttcatcggtg aatcgtcaaa
    59281 gccaatcatg cgcggcacgg ctgggcgccc tgcttccctc caggctcttg ccgcgccgaa
    59341 agcgatcaag tcgctcgtgc cgatgacgag gtctggagcc agccccatgg caagcagctg
    59401 ctgggcagct tgcaggccga tgttcagatg atcgcccgcg ttcggcgaca ggaccaggtg
    59461 atcttggatc ggcgcgccag ctgcagcgaa gccaaagagg atgcccgcga cgcgatcgcg
    59521 cgtggctgat gacgtcttcg gtgcgtggat gagtgcggtg ttctgcaggc ctagcttgat
    59581 cgcccatcgt gcgacatctt cacccgcctg ccggttgtca atgccgatga aagagccggc
    59641 ggcgcctgta gggtttcgcc ggttgacgaa cacaaccggc gttgtcgaac cttgcagacg
    59701 ccgcagcgtc gggctttcca ccgcgcctag caagacgatg gcgcgggcct gttgcgccag
    59761 catctcccga aggtattcat cctgaaggtc aggctggtcg tgcgtatcgc agagaaccat
    59821 cacaaggccg cttctacgca aggcagtttc cgcggaagca gcaattgccg ccattgccgg
    59881 gttggagagg ttcgcggcca gcaccgcaac caaacggctg gtgcgctgcc tcaggtcacg
    59941 ccccgcgctg gtggtgcgat actccagcgt ggcgatcgcc ttgcgcacgc gttccaccgt
    60001 ttcggccgat gccttgttcg ccacgccgtt caccacgcgc gagacggtgg caatcgaaac
    60061 gcccgcaagc gctgcgacat ccttgattga actcgactgg gctgttcgtt tcgtcacgca
    60121 gcttgaagtt tttcgtgttg atgatagggt tgcaataagg aaaacgtttt ccttcgtttt
    60181 ccctgaaagt caagaaccgg caatccggca tatctcagcc tagggaggaa cagatgagca
    60241 tcctacgtac acttgcatgt gccgcctttg cggccgttgt ctccgctctg ccggcgaagg
    60301 cgcaacaact gactgtttgg catgatctcg gcgacaatgg gatcaaatgg tttgaggcgc
    60361 tgtcagcgga gttcgccaag acgaagccgg gcgttactgt acgttcgcag agcttcccga
    60421 ccgaccagtg gtttggccgt gtcatcagcg cgttgaacac ggatactgcg ccggatctga
    60481 tcttcaacaa ttacgagcgc gtcatccgca tccagaacca gaccagcaag gtgatggatc
    60541 tgaaggcaat gaccgctgcg attggcgata aggccttcct gtctgaagac gatatgcgag
    60601 tcgcgacctt taagggccgc cagatcatca tcccgatcca gcgcgttcag atggcgctgg
    60661 gtgtacgcaa gagctggctc gacaagaatg gcggcaaatt cccgaacaca tgggaagagg
    60721 tgaagacgct cgctgctgct ttccgcgaca aggatccaga cgggaacggc caagccgata
    60781 cgttcggttt ggcgctacag gccgccaagc cccgcgatct catccatatg ctcgatctct
    60841 tcattttcgg ctccggtctc cgccacacgc tgatcgaccc cgagggcaag atcaccatcg
    60901 accagccggc gcatgcgcag gtgctggagg agttcctgaa gacctactcg agctacaagt
    60961 ttgtctcgcc cgacacgatc aatcactcct tcgcggagat gtatcagatc atcgaaggtg
    61021 gccgcggcgg catgttccgc gtcggcgact ggaacgtgcg caagtgggac gggccacagg
    61081 tgttgaatgg cgacttcgtt tcgggaccct ggccgaagtt cgcggcagat cgtccgaacg
    61141 ctgtcgtgat cggtggcatg cgcggtgtcg cgatcccgga gaattccccg aataaggctt
    61201 tggctcagga gttcgcggcc ttcatgctga cgaaagctgc gcagcaggcc tcgcttgaaa
    61261 acattggtgc cggcgtgcgc aaggatctcg acgtctcgaa actttcggag cgttcacagt
    61321 acttcgccaa ggccgaacac cggctggtcg cttatgattt ccccgaatcg atccacacct
    61381 tctatccgga actcgaagct gccttccatc gcaagctcct ctccggcatt gccaatcctc
    61441 cagctgactg gaaggccttc attgccgaaa cggcgaagga gatgcgtgac ctcgcggcca
    61501 agttgaaggg ctgatcccgc tgcacgaaaa tccgccggcg agaaatcgcc ggcggcccgc
    61561 caaacaggac acgcgccgat gatggatgct ctcgggggcc tgtcgcgtga cagggtattc
    61621 tatctctttg tcgccccctt cgccgcactc acgatcctgt tcggcgtctg gccgatcctc
    61681 ttgtcgatcc aggtttcgtt cacggcgtcg gcgaccgccc tcaggccaaa tccaacctat
    61741 gtcgggctgg ccaactatat cgccgttctc gctgatccga tcttcctcgc gtcgctatcg
    61801 cggacgctgc tttacaccgt gctgtccgtt atcgcgaacc tcgccttcgc actcggcttt
    61861 gccatcctgt tcgactcgtc actactcaag cacggcaatc tcttcttccg tttggccatc
    61921 ttcctgcccg tcgtgacgcc ggatgttgcg ggctatgtcg tctggcgttg gctgtatgat
    61981 cgctcgttcg gcgcgatcaa tgccgctttg aaaatgctag ggcttccggc cttcggtggc
    62041 ctctccagcg tggacaccgc gatgctgtcg atcctcatcg ctgagctttg gcatcacgcc
    62101 ggcttctaca tgatcatctt ccttgccaac ctctcgatcc gcgaccgggc gctggaggaa
    62161 gccgcgcata tcgacggtgc caccacgtgg cagcgctggt ggcatgtgat cctgccgcag
    62221 cttcggccgg cactcgtcat caacattgtc tacgcgctga tccagttcct caagaccttt
    62281 accgtcgtcg tcgtgatgac caagggcggt ccgaacgatc agacgaactt cgtgagctac
    62341 tacgcctacc agttgttcga ccaggcgcga tacggcgagg caacggcaat ggcgaccatc
    62401 ctcttcgcta tcgtgatcgg gctttcgctg acggcctacc ggataggcga cagggagcca
    62461 ggcaaatgag cgatgacatt cgccccaacc gcctgctgca gatcggtgcc tatgccgttg
    62521 cgatcctcac aagtgtgctg ttcctctatc cctattggtg gatgcttgtg tcggcttttc
    62581 gtccgacgcg ggcgatcatg tccgcgccct tgcgcccatg gccggagaca ctgagctttc
    62641 agattttctc ggagatcggc cggatcggcg gggtgaatct gtcgacttat gcggtcaatt
    62701 cgctggcaat cacaacgctt tcgacgatcc tgggcatcat cgtcaccgcg ctcggagcct
    62761 atgctctcgt ccggaggccg aaggcgcccg gcttcgcgct cgtgcgctac gggtttctca
    62821 tcacgatcat gtacccctac atgctgctcg tgatcccggt ttatctcgtg atgttcaagt
    62881 tggggctgct cggcagctat gccggcatca tcctcttcct cgcgctgggt ccgctgcaat
    62941 tcttcctgtt cgagcagttc tttcgaaaaa tcccgaacga ggtgatcgag gctgccgtga
    63001 ttgatggcgc cagtgaatgg cagatcctga cgcgcgtcgt cctaccgatg gcgatgccga
    63061 tcaccgcaac ggttgcgctc atcacattcc tgctcaactg ggcgcaatgg ttcccgatca
    63121 tggtgatttc gcggtctcct gacacgtaca ccctgccagt cgcgttgttg tccatgaata
    63181 gcgaactcgg cgccaacttt caggggatca tggcgctggc tgtgctgatc actctgcccg
    63241 ttgcggcgct gttcctgctg gcgcaaaaac gggtgatgga aggcatggca gcgggcgcgg
    63301 tgaaaggatg acgcgatgct gaagacgatc gacagccgcg agaagctcgc gatcgggatt
    63361 gataatgccc gcgcagccac actcgattgg ctcaaggcga tgcaagccgc gggcaagccg
    63421 aagggctgca tgcggatcag tgcggtgcat gacgccgacc gctggccagg catcctgctg
    63481 cctggaacat ataacggtat catgtgtctc gacctgctag gcggcctcgc ggactggtct
    63541 gcggacgaac gtgctgctct cggagcatgg cttgaagcga gccgtctccc tgatgggcgt
    63601 ttcagggtcg agggcatgca cgaggccgac gtcttcaaga agccaagcat tgacgaaacc
    63661 tggcgctaca tcgatttcca cgtcaccaac tacacgctcg gcgcgatcga ggcgctcgca
    63721 ccggaccgcc ggccgatcct tgagttcgcc aaggactacc tggacgaaaa gacgctgggc
    63781 cattggctga gcctgcgcga cctgcgtgac ccctggcagg agggcaacaa catcgtcaat
    63841 ctggccagct ttctgctgct tctgcgccag catggcagcg gacatcaggg cggctccacg
    63901 tggcaagctg aagtggatcg cgcgctggag cagcttttcg cgtggcatga ccgcttgcgg
    63961 gagccgacaa ccggcttctg gggtgttggc cagctcagcg acacgacgca actgctccac
    64021 gcctttgcgg gatcgatgca caatttccat atctggtatc acgagaatcg tgcgctggag
    64081 gggcaggatc gtgcggtcga ctattgcctg tcgttgccgc agcgcatcga cagtgcctgt
    64141 attgatgtcg acgccgtcga cgtgctcgtg cacggatggc gactgaccga tcatcgccgc
    64201 aacgacattg aggcgtggct cgcggcgctt ctgccaaagc tgcttgcatt ccagaatccc
    64261 gatggcggct tttgcgatgt gcgcagcggt gtccgtcgac aggacggctg ggtgaacggc
    64321 tacgaggagc cgcaaggcca ttccaacacc tttgccacct ggttccgttg gatcgccatt
    64381 gccatgattg cggacgtact ctggccgggg cgctggaact ggaagtttcg tcgcatgata
    64441 ggcatcgggt accggaccgc aaactggact gcggcggcat gactgcccat ccggcggacg
    64501 accctttcgc acatctgcac gatgaggact atgcgcggcc gtttgagcag cgcatggcaa
    64561 cttcgaaaac gctcgtgacg ctggcgggcc gcaagaccat ttcgctggac ggccaatggc
    64621 gcatgactct cgatctcttc gatgaaggac tgcggcagcg ttggtttgca ctcgacgaga
    64681 tgccgccttc gcaatgggcg acaccgcgcg actacgagat cgaggccgga gagcttgtgc
    64741 cggtgccatc ctgctggaac gtactcaaac cggaatggac ctactttgaa ggtgctgcgt
    64801 ggtacacgcg ctggttcgat tggcagccgg gcgaggcgga tgagcgaacc atcctgtcgt
    64861 tcggcgcggc gaactacgcg gcgctcgtgt tcctcaatgg cgcgcatgtt ggcgggcatc
    64921 gcggcggctc gacacccttt tgcatcgaag tctcggcgca cctcaatccc ggtcgcaacc
    64981 gcctgcaggt ctatgtcgag aatcgccgcc ggcctgaccg ggtaccaatg caccatatcg
    65041 attggttcaa ctatggcggg ttgtatcggg aggttgcgat cgcgcgcctg ccacaggtct
    65101 tcatccgccg cgccagcgcc atcctgctgc aggatcgcaa gagcattcgc ttcgacatcg
    65161 cgctcagcga tcccatcgaa ggagaggcgc gggtgagcat cgccgagctc gcacttgacg
    65221 tgaccctgcc ggtgaaaggc ggcgtgggca gcgtcgtgat ccctgcatcg ccagaactct
    65281 ggtcaacgga atcgccaagg ctttacgatg tgcggtttgc gttcgcgaac gacgtggtga
    65341 cagagcgcat cggtttccgg accattgcca cgcgaggcac agatatcctg ctcaacggga
    65401 gcgttatctg gctgaagggc gtttgcgttc acgaggatga tcttgaacga ggcaaggtct
    65461 cctccgaagc ggacatccgc cgccgtttcc gacatgcgaa agagcttggc tgcaattttc
    65521 tcaggctcgc acactatccc catcacgacc tggtcgcctg cattgctgac gaggagggct
    65581 tgctgctctg gtccgagatc ccggtgtatt gggcaatcga ttttgccaat cccgacacac
    65641 tcgcggatgc gcgtaaccag ctttccgagc tcatcctccg ggacatcaac cgcgcaagcg
    65701 tcatcatctg gggcgtggga aatgaaaacg cagacacgga tgcccggctt gccttcatgg
    65761 gcgagttggc tgcaacagca aagcagcttg acccttctcg gctgacatcg gccgcctgcc
    65821 tcatcaaccg cgaacacttc atgatcgagg atcggcttgc cggtcatctc gatgtgatcg
    65881 gcctcaacga gtattttggc tggtacgagc cggacttttc cggactggac cggctccttg
    65941 ccaattcgaa cccagacagg cccgtgatca tcagcgagac cggcgcggat gcagcgcccg
    66001 gacatcgcgg ggcggggcgg atgctttttt ccgaggactg gcaagccgag ttttaccgcc
    66061 agcaattcca gcgcattgca gcgacaccct tcgtcgcggg gattgccgcc tggttgttgt
    66121 acgacttccg caccgagcgg cggcaaaccg ggtttcagca aggcttcaat cggaagggtc
    66181 tgattgaaac tgacaaatcg acaaagaagc tggcatttga cgccctggcg gactgctacc
    66241 gccgtaaggg accgtcgtcg tagtggcctg ggatccggcc acatgcgtgc gcgttctggc
    66301 tgccggttgc ctgccctagg gcgatcgctg tctggcatgt cgaggccagc cccctgccga
    66361 ctgccggttt gccgaccgag tggatcgccg caagaggtgg gaacaggtct ctcaacatgc
    66421 gaacccgttc tcgtgtcgtt cagcctgaaa aaagtgacat attccgactt ccaggcccag
    66481 aattggcgtg gaatttgggg gtctaaacgc ccgatttttc agccctgtga gaacgcgttt
    66541 tcggggccat caccgctaag ccccggacac ttcggggcta tatatttcaa ctaagtcatt
    66601 aatatcatta gataaaatag gtggtgcccc tagccggaat cgaaccagca ctccttgcgg
    66661 aaaccgattt tgagtcggtc gcgtctacca gttccgccat aggggcatcg ccaaggatgg
    66721 cgtccccggc gaagcaggcg gactatagcc accgttcgtc ccgggtcaac gcattgtcgg
    66781 gctccgcgtc ccagccatga acagagggtc ttgcagcgga ttgcggcttt acctcgcgcg
    66841 gccgggcctg tatccgaacg ctatgctcag acgtctctac gattggacca tctcgctcgc
    66901 ggaatctcct cgcgccacgg ttgcgcttgg cgcggtctcg tttgccgaaa gctccttctt
    66961 cccggttccg ccggatgtga tgctggtgcc gatgtcgctg gcgaagccaa aaaaggcttt
    67021 gttctacgcc atgctctgca ccatcacctc ggtcgcgggc ggcatcctgg gctacgccat
    67081 cggcgcgctg ctctacgaca cgctcggcaa gtggatcgtc gaactctacg gcatgaccga
    67141 caagctcgag catttccggg acatgtaccg gcagtacggc gcctggatca ttctgatcaa
    67201 ggggctgacg cccatcccct acaagctggt gacgatcgcc agcggccttg ccggctacga
    67261 tttcctgatg ttcgtcgtgc tgtcgctgat cacgcgcggc gcgcggttct accttctggc
    67321 cggaatcctg aactacttcg gcgaacccat ccgcaagttc atcgaggcca acctcacgtt
    67381 aatggcaacg ctggtcatcg cggcggtgct cggcggcttc tacgtggtga agttcctgtt
    67441 ttaggcggat cgcatgcgcg tcgttacggt gcgagcagac gtcctctggc tgggcctcgc
    67501 gggtgccctc gcgacggcgg ctctgctggg cgcgtggatc ttcgaattgg gattgggcta
    67561 tctgccctgc aagctctgtc tctggcaacg ctggccgtat tacatcgggc tgccgctggc
    67621 gctgctcggg ctgttcatgg cctggagcgg cgcggaccgg cgagtggtgc tggtgcttgg
    67681 cagcctgttt gccctggtta tgctcgcggg cgcggcgctt ggcgtctacc acgccggcgc
    67741 ggaatggaaa ttctggccgg gaccaacgga ctgcggcggg cgcatcggcg ccatgccggg
    67801 ctcggtcgat gactttcgca agagcctcac gacggcccgg gtggtccgct gcgacgaagc
    67861 tgcgctgcgt gttctcgggc tgtcgtttcc aggctggaac gttctggttt ccgttgcctg
    67921 tgtggccgcc accatgctgg ggcttcgccg catcccctga ccggcgatca cggctccagt
    67981 tcgctgtccc agtagagata gtccatccag ctctggtgca ggtagttcgg cggaaacagc
    68041 cgcccattgg cgtgcaggtg ctgaagcgaa ggctgaaacg gtgcttgccg cggccacatc
    68101 tcgatgtcca tcggcaaccg gtcacccttc tttagattgc agggagaaca ggcggcgacg
    68161 acattttccc agaccgtcag accgcctttc gaacggggaa tgacatgatc aaacgtcagc
    68221 tcttcgcgcg agccgcaata ctggcaggaa aaccggtcgc gcaggaagac attgaatcgg
    68281 gtgaaggccg gcgcgcgcga cggcttcaca taggtcttga ggcagaccac cgagggcaag
    68341 cgcacctcga aggacgggct gcgcacgacc cggtcatatt ccgagacgat atggacgcgg
    68401 tcgagaaaca ccgccttgag cgcgtcctgc caggaccaga gcgacagggg atagtagctc
    68461 aacgggcggt agtccgcgtt gagcacgagg gccggacagg cgtccggcga aacggcaggc
    68521 gggacgtgaa ccgtcaaacc ctcgtctcct cggtcgaatc ggcgagatcc gaagccagac
    68581 catcatctaa gcgcctcgtg acgcgcttgt gaaggtggta gcgtcacgcg aaaccgacgc
    68641 atggaccgtc ccgtagagct gtccgatccg ccgcagaacc gcagcaagag gctaggcgtg
    68701 gccagccctg ccgcccccct cctcgaacgc cactccggcc tcgccgaagt tgcgccacac
    68761 cacccgcgcc gcgacacggc gccccttgca cggcatgacg agcgtgaacc ggttcacaac
    68821 gcttgcggta ttgtggaacg cgatgcgcgc tccgcccgtc gagaggtcgc gaacaatgca
    68881 gttcatcgtg ccgagcggat acggctgttc gatccgcgca ccgatgtagg ttttcaggcg
    68941 ggcctgctga cgtgcatcct gcatggctat cagtccttct gactgaagca tgcgcaccca
    69001 tcgcccattc gtccagaata acccaaaatt aacctgaacg catcgacgcc gaccaccggt
    69061 ctcaacgggt gggaaccggt ttctccccgc gatagtcgta gaagccgcgc ttcgtcttct
    69121 tgccgagcca gcccgcctcg acgtatttca ccagcagcgg acagggacga tacttcgaat
    69181 ccgacagtcc gtcgtgcagg acctgcatga tggcgaggca ggtatcgagt ccaatgaaat
    69241 ccgccagctc cagcggcccc atgcgatggt tcgctccgag cttcatcgcc gtatcgatgg
    69301 cttcgactga cccgacgccc tcgtagagcg tgtagacggc ctcgttgatc atcgggatca
    69361 ggatgcggtt gacgatgaag gccgggaaat cctccgacac ggtgatcgtc ttgccgagcg
    69421 tcgtgacgaa cgccttggcc gcccggaacg tggtgtcatc cgtggcgatg ccgcgaatga
    69481 gctcgaccag ctgcatgacc ggcaccggat tcatgaaatg gatgccgatg aaccgctccg
    69541 gacgctccgt ggtcgaggcc aggcgcgtga tcgagatcga cgatgtgttc gaggccatga
    69601 tcgcctcggg cttcatcgtc cggctcagat cctggaagat cctgcgcttt acggactcgt
    69661 tctcgctcgc cgcctcgatc accagatcgc aggaggaaag atcggccagc gtcgcagccg
    69721 gcgcgatgcg atcaagcgcc gccttcttgt cggcgtccga gacgacgctc ttctgaacct
    69781 ggcgggacag gttgccgttg atggtcgcaa gaccgctgtt gatgcggtct tcgctgacat
    69841 catgcagcgc gacgccatag ccggccagcg ccgcgacatg ggcgatgccg ctgcccatct
    69901 ggcccgcgcc gacgatgccg atcttccgga tctcgaatgt catgccctgc cccgctgacg
    69961 gtccattgcg ccgtcgataa gaagaaaggc cgccgcgccc tcgcgcaacg gccctgagtg
    70021 tcaccccttg atcttggcga gttccgcttc aagcgcgggc atcacctgaa acaggtcgcc
    70081 gacaagtcca tagtcggcga cctggaagat cggcgcgtcg gcatccttgt tgatggcgac
    70141 gatcaccttg gaatccttca tgccggccag atgctggatc gcgccggata tgccgacggc
    70201 gacatagagc tccggcgcca cgaccttgcc ggtctgaccg acctgccagt cgttcggcgc
    70261 gtagccggca tccaccgcgg cgcgtgaggc gccaacggcg gcgccgagcc tgtcggcgac
    70321 cggctcgata tacgtcttga aattctcgga ggacgccagc gcgcggccgc cggagacgat
    70381 gatcttcgcc gaggccagtt ccggccggtc cgacttggac agcgcgtcgc ccttgaaggt
    70441 cgagacgccc ggatcggccg cggcagcgac cgcctcgacc gccgccgaac cgccgcccgc
    70501 agccgcctgg aacgaggcgg tctgcacggt gacgaccttc ttggcatcgt tcgatttcac
    70561 cgtctggatg gcgttgccgg cgtagatcgg gcgctcgaac gtgtccgccg agatgacctt
    70621 ggtcacggcc gagatgagca tcacgtcgag cagggctgcc acgcgcggcg cgatgttgcg
    70681 cccgttcgcc gtcgcaggcg cgacaatggc gccgtagccg ccggcaaggc ccgcgaccag
    70741 agctgccgca ggttccgcca gcatgttcgc ataggcgtcg gcatcggcga ccaggacctt
    70801 ctcgacgccg gcgaggccgg ccgccgcctt ggccgcaccg ccgcagttct tgccggccac
    70861 gagcacatgc accggcgcgc ccatagcagc ggcggctctc agcgtcttgg cggtcgcgtc
    70921 tttgatcgag acgttgtcgt gttcggcgat cacaagggtg gccatggtca gaacactccc
    70981 gcttcgttct tgagtttgcc gacgagctcg gccacatccg cgaccttgac gcccgccttg
    71041 cgcgtggccg gttcgctggt cttgagcacg ctcaggcgcg gcgcgatgtc gacgccgagc
    71101 gcctccggcg tggtctcgtc gagcggcttc ttcttcgcct tcatgatgtt gggaagcgaa
    71161 gcgtagcgcg gctcgttcag gcgcaggtct gtcgtcagca cggccggcag cttgagcgag
    71221 accgtctgca ggccgccgtc aacttcgcgc gtcacgtcaa cggacccgtc gccgatcgtc
    71281 gccttggagg cgaaggtgcc ctgcgaccag ccgagcagcg ccgccagcat ctggcccgtg
    71341 gcgttggtgt cgtcgtcgat cgcctgcttg ccgcagatca ccaggcccgg ggcctccgtg
    71401 ccgacgacag ccttaagaag tttagccacc gccagcggct ccacggcctg atccgtcttc
    71461 accagaatgc cgcggtccgc gcccatggcg agaccggtgc ggatcgtctc ctgcgcctgc
    71521 gcaggcccga ccgacaccag cacaacctcg ctggcctttc cggcctcctt catgcgcagc
    71581 gcctcctcga cggcgatttc gtcgaaggga ttcatgctca tcttgacatt ggcaagatca
    71641 acgccggatc catccggttt gacgcggatt ttgacgttgt agtccacgac ccgtttcacg
    71701 ggcacaagaa tcttcatggt tcgctcactc ccggcggcaa gctttcgcgc gctacattct
    71761 ggcttctgcg ggccgtgacc ctagcggcgg cgaatcgcaa ggtcaacgga ttatgctgca
    71821 tcgcaataaa tggttcaggt gatatgtgtc cgacgccgca acaccggccg agcgggcagc
    71881 atgtccttgc ggaacgggat gacgaggacc gctcccgcga tcaggccgac gacatgcgcg
    71941 atccaggcga cggagcggtt gggatcgaag acggcatggc caacctgaag ggcgaaccag
    72001 ccgaggatga accagaacgc ggggatgcgc accgggatga tgttcaggaa cagcccgaaa
    72061 acgcgcacat tcggatgcag cagcagataa ccgcccagca cgcctgaaac cgcgcccgag
    72121 gccccgacca gcggcgcctg cgaggtgctt gccgtcagcg catagaccag ccccgcgatg
    72181 atcccggtca gcagatagaa gaccgcgaag cgcgcgtgcc ccatctcgtc ctcgacattg
    72241 tcggcgaaaa cccagagaaa cagcatgttg ccggcgaggt ggaccaggtt cccgtgcagg
    72301 aacagatgcg ttaccggggt aagccaggcc ggcgcgtgca cgatgcgggg gtccagcaca
    72361 tccgtgccga acacgacgct cggtatgagc ccgaacccga gtgaaacctg gggttcaagc
    72421 cgcaccagaa agtcgctctg gaacagcacg aagatggcgc aggtcacgcc gatgatgccc
    72481 cacgtcaccc acggcgaggc caggcggcgc agcggttccc cgtcattgat cggcagaaac
    72541 atggctgccc cctcccggct cattctggca ggtcatcgga cacgcgcaag gggacccgca
    72601 cccgccctac cggttttggc ctggcgtcca gagcacgtcc gcccggcctt cgtcgttcac
    72661 gatgcgcgcg gcgacgaaca ggaaatcgga cagacggttg atgtagtgca gggcgggcgt
    72721 gccgacggtc tcgccgggtt ggcgcgccag cgtcaccatg gcgcgctcgg cccggcgggc
    72781 aacggtacga gcc
//
LOCUS       DAOMXN010000002        56022 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_423_length_56022_cov_11.789572, whole genome shotgun sequence.
ACCESSION   DAOMXN010000002 DAOMXN010000000
VERSION     DAOMXN010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 56022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 56022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56022
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_423_length_56022_cov_11.789572"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="PK812_00390"
     CDS             <1..747
                     /locus_tag="PK812_00390"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRJ68037.1"
                     /translation="QAQAPAPAPVGNSRLAEVQRRGTVRIGTTGDFNPMSFRNPGSNE
                     YVGFDIEAMTQFAADLGVKIEWVPTEWATIVAGIAANRFDIFSGASVSMGRAKTAAFS
                     VPYLEAGTVPLSLKTNAGRFKTWESINAAGVKVAVSMGTVFNDQAKAHFPQATIVAVQ
                     APATGFQEVLAGRADVTITSNVEASTLIKRFDQLAINVPGAEMRNKRPFAYVMPLDDV
                     SWLNYVNTWVTLKRIEGYFAALEKKWLPSA"
     gene            729..1517
                     /locus_tag="PK812_00395"
     CDS             729..1517
                     /locus_tag="PK812_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014283927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRJ68038.1"
                     /translation="MAAFSVKTTISRAATAGLVLAAALALGGCSGTTYSWGWHVVSPL
                     DQRGLNNLIFLASGVWASLLIALVSIVFSVGIGLAVGIAALSKQPWLRTVNRLYVEIF
                     RAVPLLVLLLWVYYGLPVVAGIQFGVFAAGVISLALSDSAFEAEIFRAGIQSIPKGQW
                     EAGRSIGLTSWQVMRLVVLPQAIRRILPALGNQFVYVLKMSSLISVIGYQELTRRANE
                     LTLIEYRPLEIYTLLVLEYLALILVVSWLVRKLEQRMAAGDHGS"
     gene            complement(1523..2293)
                     /locus_tag="PK812_00400"
     CDS             complement(1523..2293)
                     /locus_tag="PK812_00400"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HRJ68039.1"
                     /translation="MRLELMTSPAVADYLTRRQGILIPVGATEQHGPDGLIGTDHLCA
                     QAIARRCGEEHGIVVAPTLSYGMSQFHLAFPGTVTLRPSTLAAMVHDIVASLAATGFR
                     RLYFLNGHGGNLAPLRCGIQEYHAARSLAGGEGLASVQCRLKSWWEMPAADALRKSLY
                     GAQEGFHVTPSEVALTMATHPDVIAPFDRPPPITVGEDLIVHAGDNYHDAADYRARFP
                     DGRVISHSALATRAAGEELLKLAVQDLSADFDAFCRAA"
     gene            2365..3024
                     /locus_tag="PK812_00405"
     CDS             2365..3024
                     /locus_tag="PK812_00405"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRJ68040.1"
                     /translation="MHLIQLAVSLSSFKVRAALKLKGLDLPLEDPPGGTYRSEAFRAV
                     NPAGTIPTLVDGDFWLAESDAIIDYLDDVGAGRPLRPTEFRAAARARMVSRWVDFRID
                     PQLRRLFPHVAPDARDEAEVRRVDEALAMGLELISRGLDAEGPFACGRQPSVADCGLL
                     ACMIWLDAMKAPLSLRAEADGRLAGVIAALEAVPALTSEAAAYRALAAEWVRARSHRA
                     R"
     gene            3172..3948
                     /locus_tag="PK812_00410"
     CDS             3172..3948
                     /locus_tag="PK812_00410"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HRJ68041.1"
                     /translation="MARRIVDISVPLEAGIASDPPGLEPKIEYMTHKESIPGILAFFP
                     GLRPEQLPDGEGWAAERCQISTHNGTHMDAPYHYATTQDGGKRAQRIDEIPLDWCLQP
                     GVKLDFRHLPNGHVVSAAEIEAELKRIGHTLKPLDIVLCNTAAGARYGQPDYVPTGCG
                     FGREATLYLTSRGVRVVGTDAWSWDAPFGYTAQRFAKEGDPSIIWEGHKSGREIGYCQ
                     IEKLANLDQLPSSGFMVSCFPVKVKAASGGWCRAVAIFDA"
     gene            3959..4897
                     /locus_tag="PK812_00415"
     CDS             3959..4897
                     /locus_tag="PK812_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRJ68042.1"
                     /translation="MKLATFVRKDGSPAVGVVDTERGQILDLALAARQSGMPERHFAS
                     MLALIDAGSDGLAEARRLAKTWPAAYVSLKGAKLLAPLPEPRQMRDCMVFEEHVANAY
                     KRASERTGRPLAPIPQVWYDQPIYYKANRFAVCGPDIDVTWPRYSQVMDFELEMACVI
                     GKRGKDIQPDHAHEHIFGFTIFNDLSARDAQMVEMAAMLGPAKGKDFDNANVLGPWIV
                     TPDEIGDPHALTMEARVNGERWGGGTSAAMHHRWPAILAHVSKGETIYAGEVICSGTV
                     GTGCGLELGRQLQDGDVVELEIEKIGVLKTRVLRPK"
     gene            complement(4906..7080)
                     /locus_tag="PK812_00420"
     CDS             complement(4906..7080)
                     /locus_tag="PK812_00420"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRJ68043.1"
                     /translation="MLAFLVVAGLVWLRIYDPVITEVARHRVFDNYQKLKPRAYKPAP
                     VAIIDIDEKSLTRVGQWPWARTRVAELVERLKAQGVAAIAFDIVFAEPDRMSPESYAK
                     TVAGLSDAARAELAGLPDNDSVLARAFQGARVVVGQSGTHLAGEQRDLGAVPETPVAT
                     IGGDPRPWLAQYPAWLTNVPALEQAATGRGLFSILPDPDGVVRRVPLTAIVGGRLQPA
                     LSIETLRVATGQDAFAVKRDDVGVKSVVVAGVEIPTDRTGHLWLHFTPHAPQRFVSAA
                     DVLEGKVPPGRLAGHLALIGTSAVGLFDLRPTPVDPVMPGVEIHAQALESILGKHQLT
                     RPHYAIGAEIVAAVTSSALVISLLPLLGALPTLLLGAVGAAIIGGGSWWLFANHGLLL
                     DPLFPLFTSGAAFLVLAFVNYRREEMQRQQIRSAFSHYLAPGMVEALAKDPSKLSLGG
                     ERRPLTVLFSDVRGFTSLSESYKHDPQGLIALMNTLLTPLTNAIVAEQGTIDKYMGDA
                     VMAFWNAPLDVPDHPAHAARAALAMQNAMTEVNARAQASATRDGRALPDLSIGIGINT
                     GDCVVGNMGSSRRFDYSALGDTVNLASRLEGQTKGYGVSIILGDATASALTGRFAVIE
                     LDLIRVKGKTMPERICALIGGPDLLERPDYLVAATGCNRALSAYRRQDWAEAEACIAE
                     FRARAASFGLDGYLTMLAERIALFRTLPPPKDWDGVYEALTK"
     gene            complement(7140..12203)
                     /locus_tag="PK812_00425"
     CDS             complement(7140..12203)
                     /locus_tag="PK812_00425"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRJ68044.1"
                     /translation="MYKLGGDNLDALPIVARGGTAVLASAEALFQGQFERVDQHLLIT
                     GPSGERFLVRGYFAAAETPLLASPDGAIMLPEAVRSLAGETLADMVAQAGAPAGAAPI
                     GRVDVLSGNANVQRGGSSLRLLVGDPVFKGDVVSTQGASSNLGIKFIDGTVFSLSANA
                     RMVLNELVFNPGGSNNSMAANLIQGTFVFVAGQTATNGDMKITTPVATMGIRGTTPVV
                     NINAANGLTVFSIIPDPDSNRIGTYDLLEVLTGRLIRSVSESGRAVVLASAGSVPLDR
                     PYTTAEVATSQTLQAQITSLYRSSLQSPAIDPQQPAPQPDDRTQLVPLNPLVGNIDVA
                     MTDSDRQAIDLAALDKAPKAPTAVQFAGVDAEGADKPSEFVSIGLTVPDPIVLDAIGG
                     PFLLNPLNGGSVTENGQIVFFDLAQGQFGLTISQIQVTASNGGSVAAFLFGTTLAIDP
                     AQFKYLNNGQSLTLSISYSASNGTYVASTSGQLVITGSNDNPVARSDYFAGTAPGDDP
                     GVNAYGNLLSNDTDIDSLGPFRIASVNGIVPNSPIVVHGIRGTLTVQSTTGDFVFVPN
                     SGPANRALSVRDVGSETFYYVVADPEGGQTTGELTVALQGQNDMPIGIDDTNASTAAG
                     IGAAVAATGNLLANDVDYDNGDVLLVTAVDTTPMPSGGVTPITITGYYSITDFYANGS
                     YVSTPKNIWDLSILAVGQFVTETFYYYLADGNGGITNAYLDITINGANDAPVAADDSG
                     SIIEDDVSTSGSALINDSDIDIPDTLSVTHVNGVAFTGSGSATNALGTLSINDAGNWV
                     FTLNNLSASVQGLLPGEQLLASFGLTVSDNHGATAASNVVIAIDGLNDAPTAVNDIVS
                     GTEDTLATILRTTLFANDIDPDINDTKQLYSLTAISGGSIAFSGGGANINPTAHFSGQ
                     GQFGYTLRDGAGVTSFANITIDIAPVADAPTIVLDALAPTPLAVGAAGLVNTTTTHSQ
                     YYSAVAALEGGGYVIAWQDGSGLDGSATAVIFRIYDASGNALTNEIIANSYTTGDQYR
                     PSVAAQPGGGFVLTWTSDGQDGSGQAVIGRIFDSTGNPVTAEFQLNTTYANAQTETSV
                     AALDGGGFVVAWNSENNDGSLTGVYVQKFNASGAIVNGELPVNTYTAAEQQNPVVVAQ
                     TGGGFAVFWESYNQAGANSFDVFGRFYSSTPTALTGEININSTFAGHQMKVTAAELNS
                     TNIIVVWQSQSQDSDQNGIFGKILNASGTAITSEFAINTTTVNDQMAPSVTALADGGF
                     LVTWASFGQDGSDYGVIGQRFSSNGVMVGSEFVVNTTTLDYQSPNLNETSLAQLNNGT
                     IVIPWDTVISGSTEVAQRLYALTPTGPEDTAINLGTMQFSTPDTDGSETIEVALTGFP
                     VGATFNKGAVDPGNSARWIIDGGDVNALSTIALEMTPPANWNGTFTLTVTANVTDTAV
                     LTTGTVTDTESASAVMNVVVTAVNDAPVANGETVFGFNSYIGTTIQIPYSAIQFNDTD
                     VDDANSVFLNSATSTPANPTFGPGYLEFVQQPLITGPEIITYRVSDAHGALSGYADMT
                     FQDSDDLTVMAGNTGDDIFITELAFAEEMTGGAGRDVFVFQMDTMNGASALDYTEGED
                     AIWVPVAAGAIGSGSLDDFVRVLNGSLEVDLDGTGSGYAWQWIGDLNGVATGTNDNIA
                     VLLDDSSSSFRGEIIAQFL"
     gene            complement(12321..13322)
                     /locus_tag="PK812_00430"
     CDS             complement(12321..13322)
                     /locus_tag="PK812_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRJ68045.1"
                     /translation="MSSTRALLEVSDLTKVFDVSKPWLERTLAGEGKKTLKAVTDVSF
                     SINKGETFALVGESGSGKSTIARMIVGLYPITSGKVLFDGIDVADPARAADMARERRR
                     LTMIFQDPYASLNPRWRVDKIISEPIRAFGIASDPQDIEARVGELLRLVKLDPLDGQK
                     YPHEFSGGQRQRIAIARALASRPEFIVCDEPTSALDVSVQAQVLNLMRDLQEQFGLTY
                     LLISHNLAVIRHMATRVGVLYLGRLAEVAPSKDLFRTPRHPYTRLLLAAVPDLHGIGK
                     DRVAVQGEIANPINPPSGCTFHPRCPLANERCKREVPLPRSAGAAVVACHAVEEGRS"
     gene            complement(13319..14299)
                     /locus_tag="PK812_00435"
     CDS             complement(13319..14299)
                     /locus_tag="PK812_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ68046.1"
                     /translation="MSDPLLQVKHLRVEFPTRRGTLVAVDDVSFDVSKGEVLGVVGES
                     GAGKSLTGAAIIGLLEPPGRIAAGEVVLGGRRIDNLPAEEMRRVRGRSIGMVFQDPLT
                     SLNPLYTVGQQLTETIQTHREMSDSAARARALELLNDVGIPAAADRLDAYPHQFSGGM
                     RQRVVIALALAAEPDLVIADEPTTALDVSVQAQIISLLKRLCRERGTAVILVTHDMGV
                     IAETADRVAVMYAGRIAEIGPVRDVILSPKHPYTVGLMGAIPALDTEVERLAQIPGSM
                     PRLTAIPRGCAFNPRCEKVFERCRMERPDPIPAGAGKVACWLYDTKRGQA"
     gene            complement(14299..15246)
                     /locus_tag="PK812_00440"
     CDS             complement(14299..15246)
                     /locus_tag="PK812_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008551641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ68047.1"
                     /translation="MQHTEASLPTPAPASGNRFWQSDIAHSFMRSKVTMLAALVTLIF
                     FVGALLAPWIAPNDPYDLRQLSLLNSNMPPAWVKDGSAQFPLGTDDQGRCVLSTILYG
                     LRISLGVGFVAVGLSAVLGILLGLTAGYIGGRLDSFIMRVADVQLAFPAILIALFVDG
                     TMRGLFPHIKRENSAFLILVIALTLAFWVQYARTVRGSTIVEKNREYVQAARVIGLPA
                     FTIMIRHVLPNVLGPVLVIATINLGLAILSEATLSFLGVGFPPTEPSLGAMIQIGSRF
                     LFSGEWWITVFPGAALAILVLAINMLGDWLRDALNPRLR"
     gene            complement(15246..16226)
                     /locus_tag="PK812_00445"
     CDS             complement(15246..16226)
                     /locus_tag="PK812_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ68048.1"
                     /translation="MFAFILRRLVQSLYVMLAVGVLAFTLFQFVGDPVNQMVGQDATV
                     EERAKLKQDLGLNDPIVVQFARYIGRAARFEFGMSYQFKQPVMQLILERLPATFELAM
                     MAMLLALLIGIPMGVYTGLHRDNWLSKVLLAVSLLGISLPTFFIGILLIFFFAVQLKV
                     LPSFGRGQVVDLGIWTTGLLTKSGWSAIIMPAFTLGLFQMTLIMRLVRAEMLEILRMD
                     FIKFARARGLTNRAVHFGHALKNTLVPVVTITGLQFGSIIAFAVITETVFQWPGMGLL
                     FLRAVQTVDIPIMAAYLMLIGLLFVVINLIVDLLYFAVDPRIRGAVKAAH"
     gene            complement(16281..17915)
                     /locus_tag="PK812_00450"
     CDS             complement(16281..17915)
                     /locus_tag="PK812_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRJ68049.1"
                     /translation="MFRRVKTLALASVMAAALFAPQAMAQNTFKMAFQGNLNTLDPYT
                     INETFTLAMQGNIFEGLTARNEKLEIIPGLAERWELLAPNKWRFHLRKGVKFHGGEPF
                     TADDVVFSAERVRKQDSDLKARVPKDAVFTKVDDHTVDVTMPGPNPILHYSWDTWYIM
                     SKSWAEKNNAVEPISSRAQTPGFSTLNANGTGPFKVATHQTGVKTELVKNPDWWNDKN
                     KKHNLDRVIFTPIANDATRVAALLSGELDWVDPVPVQDQERVNSNATTKMMAGGETRT
                     IYLGFDQRRDKLLAPAQVPGDKNPFKDARVRKAVYQAIDVNLIVSRVMRGQAVPSALM
                     LDPSLFPSYAKDFKRHPFDAAAAEKLLDEAGYPRVGGAQGNRFTVGMDCPNDRYVNDA
                     AICQAVIAMLARVGVKVEPIIQPRAQYFAKILAPGGYNTNFYLLGWTVGTNDPHNVLE
                     QLHQCRDDKGSGGIGVANVGGYCNAKVDELSNKILVENDIAKRNLMIKEAFEIIHNEV
                     GHVPLHQQALAWGMSKKVSIDQRADNMIMLYTVKMQ"
     gene            complement(18137..18331)
                     /locus_tag="PK812_00455"
     CDS             complement(18137..18331)
                     /locus_tag="PK812_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2783 domain-containing protein"
                     /protein_id="HRJ68050.1"
                     /translation="MPLNTRPNIPDHDDFYQELIDSQRDMSEAQAEMMNARLILLLAN
                     HIGDRETLREAIRLAGGKAS"
     gene            complement(18344..19954)
                     /locus_tag="PK812_00460"
     CDS             complement(18344..19954)
                     /locus_tag="PK812_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRJ68051.1"
                     /translation="MLKTYQFPQFPYVQSDEQKSGRPQRHPVIVVGAGPIGLAMALDL
                     ASRGIRCTVLDDNNTVSVGSRAVCYAKRPLEILDRIGVGERCVAKGVSWKVGKVFFRD
                     RLSYQFDLLPEAGHKMPAMINLQQYYLEEYMVEAIMASPLVDLRWNHKVTGLGQADDH
                     AVLTVETPDGVFMTEADWVIACDGANSTLRSMVGAQFTGHFFQDRFLIADIVMKAEFP
                     AERWFWFDPPFHRNQSALLHKQSDDVWRLDFQLGWDADPVEEKKPERVIPRIKAMLGD
                     ERDFELEWVSIYQFACRRIDNFRYGRVLFAGDAAHQVSPFGARGANTGFQDTDNLAWK
                     LKLVLAGDAPEQLIDSYHEERAFAADDNLLNSTRSTDFITPKSPTSRRFRDAVLDLAE
                     SQAFARPLVNSGRLSSPTPYLKSSLNTPDSEPFSGRMKPGTNCDDAPVRSAGKDGWFL
                     NRIGDGFGVLLFGETPPVEEVAHGGVSAKVLVVGRDFADSEGLLSRRYDARPGTTYLI
                     RPDQHVAARWRSFDIGKIRAALARAIAA"
     gene            complement(20039..20581)
                     /locus_tag="PK812_00465"
     CDS             complement(20039..20581)
                     /locus_tag="PK812_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRJ68052.1"
                     /translation="MLVEKIHHVAYRCKDAKASVEFYKKVLDMDLLGAIAEDKVPSTK
                     APDPYMHIFLDAGNGNILAFFELPNSPPMGRDPNTPEWTQHIAFQVKDMATLNAIRER
                     ALAAGLDVVGPTNHSIFQSIYFFDPSGHRLEVACWTTTSEEMKRMRDVAHAMIDEWAV
                     TKKPPRHTAWLHEKEFKASH"
     gene            20767..21234
                     /locus_tag="PK812_00470"
     CDS             20767..21234
                     /locus_tag="PK812_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRJ68053.1"
                     /translation="MSSGGGDGTLDLTRFFPYRLAILAEAVSQTMAQLYADRFDLTRQ
                     EWRIVAALGNRKRMAGRDVGRLSALDKMQVSRALARLEGRGMIDRAPDPDDRRNQVLS
                     LTAEGRALYQKIVPLVQAREEYILSALSPEEVRTLNALMTRVQARAEEMLRRG"
     gene            complement(21310..22467)
                     /locus_tag="PK812_00475"
     CDS             complement(21310..22467)
                     /locus_tag="PK812_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009758267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRJ68054.1"
                     /translation="MKRLSTILAVAAMALGISGAKAQVQGVTDSEVLVGSHNDLSGPF
                     AAFGSPAVKAAQLYFKEVNDKGGVHGRRIRFIVEDHTYQMPKATQAINKLINSDKVFA
                     MLLSLGTPMNIAAFKLQDEKKIPNVAPLTSARQMLDPPNDYKFTGFSSYYDQTVGAVK
                     YFNEKDKATKYCLMYIPSDFGLEIQQGAVDTIKKMNLTLTGETTHKPDEGDFVGPLSK
                     HKDAGCEVIVHALGVRQAITVSGTLKRMGWNAKLVGSSAGFNTVMAAVPGGVTEGLYA
                     AAGWADHVARMNVPEVKAWADGYKAAYNEDAGTGALLGRSAAETFVRALQAAGKTLTP
                     DTFKKGMESLKYTDAITGAQIAFSEKDHEGAKDIIISQVQGGKWVEVHRVK"
     gene            complement(22543..24462)
                     /locus_tag="PK812_00480"
     CDS             complement(22543..24462)
                     /locus_tag="PK812_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009758268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HRJ68055.1"
                     /translation="MNVVVTPADATSAAMPAQKMHNGVSLNADLLQRPYFVDGADTLP
                     KLFSKRCAQFGARTAHREKHFGYWQSFSWNDFWEHARLIGLALRALGMRRGDVISILS
                     EDRKEWLYTDLGAQGMGAVVNGVYTTDSASQLAYLVNDSGSRFLFVENDEHLDKYLEV
                     RDKMPTIQKVIVYEREGLYDYKDDTVLFIDDLYDLGALELKKTPTLFDDEIALSKPDD
                     TALLIYTSGTTGPPKGAMLSQSNVMFGISAALQGLETAQDDEQLCFLPLCHVYERLNS
                     AWLSIGIGSTVNFAESTETVFDNMREVSPTLFAAVPRVYEKVYSRITIMNNEATVFGK
                     LAYKLALKAGDAHARALEEKRTPSLLESLSYRLWNWLVLGNIRRMLGMERVRRATSGA
                     APISPDLLRWYRAIGVPIFEAYGQTENTAIATGNRIGNNRTGTVGPAMPGLEIRLSPE
                     GEIQTRSGGVFQGYWGKPDKTAEAMTPDGFLRTGDVGTVDEGGFWRITGRIKDIIITA
                     GGKNITPAEIENKLKFSPYISDAVVIGDRKAYLTCLIMLDQENVEKFAQDSRVPFNDY
                     ASLCAAQAVKDLIKEVVDDTNKEFARVEQIKDFRIIDVLLTAEDDELTATMKLKRSFV
                     EKKYAPLIAQMYKGG"
     gene            complement(24459..25235)
                     /locus_tag="PK812_00485"
     CDS             complement(24459..25235)
                     /locus_tag="PK812_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009758269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ68056.1"
                     /translation="MSEFPLLRVKNVESYYGPIMAIRGASLDVTQGQVVSVLGANGAG
                     KTTLMKTISGVMWPEKGEVLLDGQAIQGLEPDQIVKKHVIHVPEGREIFPLLSVAENL
                     QMGAFTRPDRQNIAADLDMVFSYFPILKERYQQRAGTLSGGQQQMLAIGRGLMAKPRL
                     MLLDEPSLGLSPLLTQEIFGIVRRLNLEQKVTIMLVEQNAKAALDISDYGYVMELGRI
                     VMAGEAEKLRNSKDIQTFYLGVQEEGARGQRRWKQRKTWR"
     gene            complement(25232..26008)
                     /locus_tag="PK812_00490"
     CDS             complement(25232..26008)
                     /locus_tag="PK812_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009758270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ68057.1"
                     /translation="MSMLALEHVTLKFGGITAVNDVTMSVKQGEVFALVGPNGAGKST
                     IFNLISRFYTPYSGDIRFEGHSLLKLAPSDIPSLGIARTFQNIELFERSTVLENLLVG
                     RHRHVQSTFWSDLAFTPFVHREERRHREAVEKVIDFLDLQAYRDKYVAGLPYGVRKVV
                     EMARALATEPKLLLLDEPASGLSMDETNDLAFWIEDIKKQMGITVLMVEHDMKLVAAV
                     SDRVLALADGKVLAMGTPKEVQSHPDVIRAYIGGSPEAAA"
     gene            complement(26005..27102)
                     /locus_tag="PK812_00495"
     CDS             complement(26005..27102)
                     /locus_tag="PK812_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009758271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRJ68058.1"
                     /translation="MRNVFKTSYDADIRLFKHGAQAFWYALLLALMLAMPLLISSFLL
                     GEVSGILIWSIAGLGLMVLTGQAGQASLGHTAFLAMGCYANTILMMKGVPFFISFPVA
                     GLLAGLFGVIVAIPTLKLHGIYLAIFTLALAVLIEDIIVLSEPLTGGVTGLTSQTIKM
                     FGLSFDRYANPNAFYYLVLATTLLVVLFYRNMLRSPLGRSFAAIRDSEISAQAMGVNI
                     ASTKAMAFFISCFITGLAGALIGQFSGAFNHETFNLVIAIQLLLMIVVGGLGSIHGAF
                     FGAIVVGAIPLGLSFLRDYIGKLSGAGSIAIPGLESGIFGLILIGFILFEPMGIYGRW
                     LKIRTYFELFPFYRRDMFRRNRSYLKTERMR"
     gene            complement(27107..27982)
                     /locus_tag="PK812_00500"
     CDS             complement(27107..27982)
                     /locus_tag="PK812_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009758272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRJ68059.1"
                     /translation="MSSFQLLVSGLANGCVYGLIAIGFVLIYKATEQVNFAQGDFMML
                     GAFIVIALSQGKMVQLPFYLSIPLAIATMAMFGYLLDMIVLRRMFGQSQTAMVILTIA
                     LGFVIRFVAGAIWGHEPVALATPISGQSAKVGGIILGLDEIAIVVVTVLLTLALYYYF
                     NRTRMGVAMQAASQNQLAAYYMGIPVKQVQSLTWALAGGTAAIAGILFATKGSIDPST
                     GLLGIKAFAAAVIGGFGSLPGALLGGLLVGVVEPFASRYVMAGYSQIAPYAVLLLVLI
                     FRPHGLFAQVYSKKV"
     gene            complement(28176..29867)
                     /locus_tag="PK812_00505"
     CDS             complement(28176..29867)
                     /locus_tag="PK812_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRJ68060.1"
                     /translation="MSKSKTPHTESMTGGMAIVRQLLANGVDTVFGLPGAQLYPFFDG
                     LAQNADRIRTYSARHEQTCGYMAFGYARSTGRPGVFSVVPGPGVLNTGAALCTAAGTC
                     TPLLMVTGQVPSMFLGRGRGHLHEIPDQLATLRSFIKWAARIERIEDVPAMVNEAFRQ
                     MLSGRPGPVALEMCWDTMAQSAPVRLLDPAAPLPAPPPQADAVDQAARILASARHPMI
                     FTGSGAQKAAQEIKALADYLDAPVVGFRGGRGVVPEDGPLGMMSYAAFKLWPHTDAAV
                     GIGTRMELPFMRWKGMMQLTDQPAAPPHIIRIDVDPQEMLRLKVHAPIVADSAEGTRA
                     LLDAVMRLRPPASPKDRKAARRRIAEAKAQARGAYEKVQPQLAYLDAIRSVLPRDGLL
                     VPELSQVGFTTYFGYEVLKPRTYVSEGYQGTLGFGFPSALGAKAAHPGRPVISVTGDG
                     GFLFAAQELATAVQERLGLITLVFNNHAYGNVLRDQKLGFGNRVIGATLTNPDFRLFA
                     KSFGVEFHAVRSPEALKKTLAKVIRTKSPVLIEVQSPQGSETSPWEFIHPTASYL"
     gene            30138..30926
                     /locus_tag="PK812_00510"
     CDS             30138..30926
                     /locus_tag="PK812_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003508915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ68061.1"
                     /translation="MNQPDGGLLVVSDVTRSFYGVHALNGVTLTVPGQGITGLIGPNG
                     AGKTTLFNCISGVIPPDGGRIVFNGHDIAGLRPDQITHAGLVRTFQIARGLPRMSVLD
                     NLMLYGAHQPGEGVLAAIAGSSAARHREQELLHRAVAIAERLNLLRVIRNPAAALSGG
                     QKKLLEIGRALMTEPKLILLDEPVAGVNPTLAREIGEHLKVLAAEGLPILLIEHHMDM
                     VARLCDRVIVMAEGRHLAEGTFDELADDPRVQEAYMGRRWRSAS"
     gene            30926..31633
                     /locus_tag="PK812_00515"
     CDS             30926..31633
                     /locus_tag="PK812_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009449273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ68062.1"
                     /translation="MADLVAQGIVAGYGAADEILKGVDFTIANGEIACIIGPNGAGKS
                     TLLKVIAGLLIPKSGSVVLAGQDVTGRQPRDIARAGLAYVPQEHNIFPTMTVRENLEM
                     GGYVEPNAVDRRIEMAFERFPVLADKRRHAARTLSGGERQMLAMAMALMVEPRVLLLD
                     EPSAGLSPLAAEKLFDAIVDINKSGLSIGMVEQNASEALAISHRGYILVDGRNSRTGP
                     AQALAADPEIRRIFLGG"
     gene            31697..32896
                     /locus_tag="PK812_00520"
     CDS             31697..32896
                     /locus_tag="PK812_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRJ68063.1"
                     /translation="MSGFDRRTLLKLSAAAAAAGAMPIRAYAQGEPIRVGILTPLTGA
                     GGNDGPRMLKAMQAVFSEVNAAGGVLGRRIETIVEDDQTNPEAAVRAARKLIEVNKVS
                     VIMGTWASAVTAAVAPVCWESKTFILTVSGADSITLLPHQGFLVRTQPTSKLQANAHA
                     EFIAGMGHKRVAVIGIQAPFAVPTKDHLDSILKTKGGSVVSHVIYEKDKPTYRSEIDQ
                     AMKEKPDFLYLNGYAPDTVVVLRDLFRANINLPKFCQSYAVPQATLDTMPPEVSEGVM
                     TGQPSADVESSAYRKAVERLGAKEIDGYEAQATDWASLAVLTMAKAKDTSGVALKDNI
                     RKISQGSGEKVYTAVDGLKALAAGKEINYEGASGPCDFTDIGDILGCRFRYMKVAKGK
                     LEFLKLV"
     gene            32963..33823
                     /locus_tag="PK812_00525"
     CDS             32963..33823
                     /locus_tag="PK812_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019567206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRJ68064.1"
                     /translation="MEALQLIVNGLMAGTILAIPAIGLTTLFAVLRFTNFALASHMTI
                     GAFAGFVANTSLGLPVIPSLLAAFVIAGLVGWLSDEFMLKPLRATGFITTAIGSIALT
                     IALENIVRFIFGNAMRGYNLPLRRDWVIHDIRVSPQQAENLVIAVVVMALLFAFLTLT
                     RTGKAMRAVADNPMLAAIKGINADMVARLVSFVGMGLAGLGGMLIGLDTTIDPLVGFK
                     TILSVFAAAVVGGLGSIPGAVVGALVIGVGEELSLLVLTPAYRTAVGFIAILLVLTFR
                     PRGILGQRAY"
     gene            33836..34771
                     /locus_tag="PK812_00530"
     CDS             33836..34771
                     /locus_tag="PK812_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009449270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRJ68065.1"
                     /translation="MEHYLVAMGVTAGLYAVMALGMNVIWGMAGLINLGMVGFFAVGA
                     YTSGLLTLKFGWPIPAGIVAGAAMGGLVGVLVALITVRLRGDYLAIVTLGFAETMRIV
                     MSNELWIANGTDGLSGIPGPFRQALGPHLFNLMFLGIVVAATTILFFLFERLSHAPFG
                     RVLRGIREDEQVVQVAGKNVIMFKVKAFAVGTAALGMAGALYGHFTSYIAPDLFAPLL
                     TLYVKLSLLAGGLGNNVGAIVGAVLVVFFLESTRFVIPYIPEISAVQGAALREILIAG
                     ALLLVLRVRARGLIPEKIANVPLPDRPHAALSAKS"
     gene            34783..35289
                     /locus_tag="PK812_00535"
     CDS             34783..35289
                     /locus_tag="PK812_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68066.1"
                     /translation="MPAKTRDVTLDDVAAIVAMTAAERDRMQVYRAVERIAAETVGFV
                     FLTTLKYNEAEACVERLHSSNEKAYPVGGRKPLSKITASHKAMDDGEVFLAATRAAVK
                     EAFFDHELIFALGSTAILNAPIRFGGRRLGTLNFCGEEGKYGPGEVRNAKILAGLLIP
                     CLLAEAPR"
     gene            35354..36589
                     /locus_tag="PK812_00540"
     CDS             35354..36589
                     /locus_tag="PK812_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRJ68067.1"
                     /translation="MALYHFKNFQMLDPEIGELAGGHEIIVEGDKIKEVSAKAIKSAK
                     AEVIDCGKRTLMPGLIDSHVHVVLSEVVIRNMEQIPLTLMTARAARLMGDMLNRGFTS
                     VRDTGGADWGLKEASEKGYFAAPRLFIAGKAIGPTGGHSDGRRRTDMGARCHCCNALA
                     FSMGIADGVSGVRKAVREEMRQGADQIKIMMSGGVASPYDPLDSLQFSLEEVNAAVEE
                     AHAFGRYVCAHAYTPEAITRAANAGVRTIEHGNLIDDASAKLMAEKGMFLVANLVTYF
                     EMKKRAAEYGMNADMLAKNDIVIEGALRSLEICKRHGVQVAYGTDLLGALQSEQSEEF
                     SIRAQVVKPIEIIRSATSIGARLLRMEGKLGCLKPGAFADLIVVDGNPLKNLKLLQEQ
                     GKHLSAIMKGGHFHKNRLN"
     gene            36597..37838
                     /locus_tag="PK812_00545"
     CDS             36597..37838
                     /locus_tag="PK812_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRJ68068.1"
                     /translation="MGTQLLLTNFDLLEPSFGEIRGGYELLIEGDVIRELSERPIRSA
                     GATRIDCLGKTLLPGLIDSHVHSIHSEVNIRFMEALPLTLLTARAATRLTAMLNRGFT
                     TVRDTGGADWGIKEALSQGHIVGPRLFIAGQSIGPTGGHSDSRRRSSSGGECFCCNGL
                     QFKSAVADGVDEVRKAAREQMRQGADHVKIMMSGGVASPYDPLDSLQFSDGEVMAAVE
                     ESKAFGRYVCAHAYSAEAITRAAKLGVRTIEHGNLIDDASARLMAENGMYLVANLVAY
                     YAMRERAAKWGMTAESLEKNEAVLQGGLRSLEICKRNGVPVAFGSDLLGELHWDQSRE
                     FEIRQEVVAPIDIIRSATLVGAEIVRMPGRIGTLLPGAFADLILVDGNPLANIALLAG
                     QGERLSLIMKGGTVHKNRLNA"
     gene            37835..38653
                     /locus_tag="PK812_00550"
     CDS             37835..38653
                     /locus_tag="PK812_00550"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ68069.1"
                     /translation="MSRLTSRRLGSLALTGAAGLSLAYILLPLFFVVWLSFFQQEIPS
                     FPPEGYSLKWFAAVPGNDRFVSGFLLSLQVAVIATAIGLALGVPAAVALVRARFFGRE
                     ALNSLLLLPLVVPGIVLGIAIYVFHVEAEILTEWHILGSLGGLIAGHVLIVIPWTVRL
                     VTAGLVGMDRAIEEAAMSLGADRFTTFRKITLPAILPAVVAAALFGFIASFGNLEMSL
                     FLVGPGRTTLPIAILQYLEWKIDPTIAAVSVLQILFIGGAMLITDRFVKISRVV"
     gene            38654..39718
                     /locus_tag="PK812_00555"
     CDS             38654..39718
                     /locus_tag="PK812_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ68070.1"
                     /translation="MARLELSSLVKRYGDLRAVSDVSLTVEDGEFVVLLGPSGCGKTT
                     TLRIVAGFVEPTAGSVRLGQRDITHLPPWKRNAGLVFQSYALFPHLTVNENVAFGLEM
                     RKMPPAEIAPRVAEALRLVRLAHLGERLPRQLSGGQQQRVALARALVFRPDILLLDEP
                     LSNLDAKLRQDVRVEIRELQRKIGLTTVMVTHDQEEALTMADRLVVMSDGRIRQVGSQ
                     QDLYERPADRFVADFVGRSTFLEGHIETPGRFITQGGLTIACSGLETGPAAIALRPER
                     IALLAGEEKADNAFEGRVEFVSYLGAQVDVHLRLNASERIVVQVPNRPDRTLPAIGEA
                     ARIGWSRETGRVFSGAATTD"
     gene            39754..40800
                     /locus_tag="PK812_00560"
     CDS             39754..40800
                     /locus_tag="PK812_00560"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRJ68071.1"
                     /translation="MTSTFHLNRRQLLGAAAGVGVTGLPGAALAQGKGKIVVGTWGGD
                     YARLLNKNIEQPILIKNGWEVVQDQAGDPQRRSKMLAEQRLPRGTSDVQGLSSINMFQ
                     MHDAGTVLPIDYSKLKNAGNLLPSMKYPWGVGHIYSGKVAVYNPKMINPAPKSYKDVF
                     DPKHGNKLALIDIQYQYTIVCAALAAGGSTTNLEPGKKLLLECKKAGMRMYPTNEAFA
                     AGLKSEEFGIGVMWKARVVQWQNAGISVEAVAPSEGTLAYVSGFVIPKNSPNKDGAYA
                     YVDAMLEKSAQEAFAVDMGYNPTVTNAVVAPDLNKRIGFTPDEIKKLVDLDYDHMNKN
                     DTALKEWWDKELKG"
     gene            40881..41762
                     /locus_tag="PK812_00565"
     CDS             40881..41762
                     /locus_tag="PK812_00565"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ68072.1"
                     /translation="MAAAAAHAPPEDGRTSILSASMLIGPATVFVVIGLLAPLAILFR
                     YSLNRFEPRRMMVEAFTAENYIRFFSDPFYTGIFWTTLRVAALCTIICLVMGLPLAYV
                     LARTQTRYKNILIMLVVLPLFVGNAVRAAGWMTLFGSKGFLNVSLMQLGVIDQPMQIM
                     FTEVAVIFGIIAVNLPYMVLTLQSVIEGVNRNVEEAAFSLGAGPVTMFRRVLLPLALP
                     GILAGTILTFILGMNAYATPVLLGGPKFKMMGPLVFGQFQLNNWPFGAAIAFVLMTAT
                     LVLTASANLMVQRRYRR"
     gene            41772..43382
                     /gene="ggt"
                     /locus_tag="PK812_00570"
     CDS             41772..43382
                     /gene="ggt"
                     /locus_tag="PK812_00570"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRJ68073.1"
                     /translation="MALERGMVVAAQPEAVEAGAEVLAAGGNAVDAAVATALTQTVVD
                     PQMCGLAGFGCMHVYDPARARHETIDFYARVPRSAKPDMWEAKLVSESDDGFGFFLSD
                     RSNEIGYGSIATPVTLRGLDLALARHGTLPLATLIQPAIRHARQGVMVRPHMAAYWGQ
                     VPTESRAPHQDFLTALPATRRIYAHEDGSIHKVGETLKNPDMARTLERIAEHGAEDFF
                     TGGIAAEIAADMRANGALLDADDVANSRPVIGAPIWGTYRGLRIASNPPPGGGVKLIE
                     MLNILEHFDLAAMGHNSPAYIATIAEAMKIGAVDRDATHGDPEFLDVPVARLTSKAYA
                     AEMAARIKRGEKTHVPRLKAREAADTTHVCVVDRTGLCVSLTHTLGTPSGVVTEGLGF
                     MYNGAMGAFDPRPGRAGSLAPGKRRMSSMAPSIVFRDGKPFFIVGAPGGTYITPGILQ
                     AILNVVDFGMSALEAISAPRVCATSDTIWITNRILRSTEAALRTMGYPVKRSPFNYYF
                     AGLHGIRVVDGVPDGAADPGRDGMALAV"
     gene            43411..44631
                     /locus_tag="PK812_00575"
     CDS             43411..44631
                     /locus_tag="PK812_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HRJ68074.1"
                     /translation="MTIGVGGSTPEKELAALKNMRGDVPPIGVDERLRRIDKAQRLMR
                     DKGIDALWLDVSSSLTYFTGLKMRRTERAHGAILPANGEIIYLSPAFEVEKLMTMVTF
                     GDKVARWEEDEDPTALVIDSIRALGVERGVIAVDEATPFFTFDGLRRAGNAFSFVNGS
                     DVTAACRMIKSDHEIALMQTAKNITLEVHKAAARILSEGMTTTEVQAFIAAAHRKAGS
                     EGDVGFNGVLFGEATAYPHGVPYPQVLKPGDMVLIDTGAPIDGYLSDITRSYVFGEPS
                     QRQREVWNLEKAAQAAGFAAAKPGNRCEDIDIAARGVIAAGGFGPGYATPGLPHRTGH
                     GIGLDVHEWPYLVKGDRTVLQPGMTFSNEPTICIYGEFGIRLEDHMVITETGARWFTR
                     PAHSVDDPFGLDVG"
     gene            complement(44725..45675)
                     /locus_tag="PK812_00580"
     CDS             complement(44725..45675)
                     /locus_tag="PK812_00580"
                     /EC_number="1.13.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007820775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018577 - catechol 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0009712 - catechol-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catechol 2,3-dioxygenase"
                     /protein_id="HRJ68075.1"
                     /translation="MTEPCHDIAHLGHVEMLTNKPQESLDFFVNVLGLTESGREGESV
                     YLRGWDDYEFHTLKLTASRTTGVAHIGYRASSEAALLRRVAAIEAMGCGIGWTEGDLG
                     HGRAYRFRDPDDHIFEVYFDTRKYEAPPAERPSLKNQAQRQHGRGCNVRRLDHLNLLA
                     QDVDAIRDFIPKALGSRVTEQILLDSGKLGGCWFTVNNKTYDIAYTRDMTSSRGRFHH
                     VTYAVDQREQVLQAADVFLENGVYIETGPHKHAVQQTFFIYVWEPAGNRVEVANAGAR
                     LILDPDWQIVTWTETERKKGQAWGLKTIESFHTHGTPPVG"
     gene            complement(45681..46652)
                     /locus_tag="PK812_00585"
     CDS             complement(45681..46652)
                     /locus_tag="PK812_00585"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRJ68076.1"
                     /translation="MLTRRMLIAAAAAAATGAAPLRAQARYPDKPIRLIVPFGPGGLA
                     DITSRVVGDRLSQVLGQRIVIVNQPGAGGVAAAQTALTGPADGYTLVLFTNGTAISVP
                     LVKELGYDPLTQFAPVSSLGFFDFLILTSGDHPHKTLGDLLAAAKARPGALNIGTINV
                     GSSQNLSANLFRSMANVSMEIVPFRTTPDAITALLRKDVDLVIDGYVAAKALMQEGKL
                     RALATSGPRRFAGLPDVPTVAESGVPGFDVTSWNAVFAPAGTPAEVVRVLNEAIVSSL
                     GDAGVRQRLLDLGIEPGGGTPAAIGERLKTDIARWGEVIRRAGIQQQ"
     gene            complement(46646..47506)
                     /locus_tag="PK812_00590"
     CDS             complement(46646..47506)
                     /locus_tag="PK812_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007757940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRJ68077.1"
                     /translation="MSCGKALERRPLYTPPANACDAHCHVFGPAGRFPYAPDRRYTPQ
                     DAPKEALAGLHARLGLSRAVIVQASCHGTDNRAMLDCIASAPETYRGVAIVDDTFGDD
                     AYAVLHEGGVRGVRFNFVKHLGGAPDMDVFHRVIRRIAPLGWHVVLHLDAPDIEALSP
                     MIAALPVIAVIDHMGRTPAKDGIAQEPHRHLTGLLRHAHVWVKVSGAERIADPPFHAA
                     IPFARALMNVAPDRVLWGTDFPHPNLKHEVDEADLVDMLPEYGDAKALHRLLVENPAR
                     LYGFQGDQKC"
     gene            complement(47506..48606)
                     /locus_tag="PK812_00595"
     CDS             complement(47506..48606)
                     /locus_tag="PK812_00595"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HRJ68078.1"
                     /translation="MKRKPPFRADMVGSLLRTQALKDARSKREAGQISQGDLKKVEDA
                     EIKALVKKQEEIGLQAVTDGEFRRAFWHFDFLEHLTGVEGVESAGIAFKGVTTKGHSI
                     RVVNKIDFPTDHPHIAHFKYLAKVTGKVPKMTIPSPSMLHYRGGSASINATVYRDMDE
                     YFHDLGKAYAKAVKTFYKAGCRYLQLDDTSLSYFCDPEQRDMLSKRGDDPARLMTAYR
                     DVINMATKDRPDDMVITTHTCRGNFKSTFIASGGYEPVADMLFNEIDVDGYFMEWDDE
                     RSGGFEPLRFLPKRGKHVVLGLVTTKVGDMERKDSLKRRIEKAAKFAPLSQLCLSPQC
                     GFASTEEGNLLAEESQWKKLQRVVEVAKEVWG"
     gene            complement(48603..49355)
                     /locus_tag="PK812_00600"
     CDS             complement(48603..49355)
                     /locus_tag="PK812_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ68079.1"
                     /translation="MAELLEIDALSAGYGAAIVLHDIALTLGEGQSLALLGRNGVGKT
                     TLVNSIIGVTRRHGGRIRLGGQDISAKPPEARALAGVGWVPQERNVFRTLSVTENLTA
                     VAIPGPWTPQRVFQLFPRLAERKTNLGSQLSGGEQQMLAIGRALVLNPRLLLLDEPTE
                     GLAPIIIQELLAALTKLRREEGLASIIVEQHARTILGLTDKAVILERGRIVHASQSAA
                     LLTDAAPLERYLGLAAADDGKTDHLARRGDMT"
     gene            complement(49348..50100)
                     /locus_tag="PK812_00605"
     CDS             complement(49348..50100)
                     /locus_tag="PK812_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ68080.1"
                     /translation="MSVVLRTQGLTKRFGGIVASDDVSFTLEQGARHALIGPNGAGKT
                     TLVNLLTGVITPSEGRIEMNGLDITSLSREARVKLGLARTFQINQLCAGLTVLEMIMM
                     VVTERLGHGGRLFGRLDADRAAISETAAILERFRLADVMDERVDTLPYGRQRQIEIAS
                     AFAVRPKVLLLDEPAAGVPEAERKLLLDSVAELPEDVSVLLIEHDMDVVFRFATRITV
                     LVNGRVLTEGLPQDVAADPDVKAAYLGEAMHG"
     gene            complement(50097..51107)
                     /locus_tag="PK812_00610"
     CDS             complement(50097..51107)
                     /locus_tag="PK812_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRJ68081.1"
                     /translation="MNAPSLESRIRTSLTLSRRWSPFESLFWLFALAAFFIFPRQLLI
                     LNEIAILALFAISLDLILGYAGIVSLGHAAFLGFGAYGAGLLALHVTADPIAGLGFAI
                     VISAALGLVTSPLILRGNDLTRLMVTLGIASLLHELANSLGKWTGGADGLQGITMQPV
                     LGRFEFDLLGKTAYLYSLAVLFILFVIARRIVHSPFGLSLRAIRDNQLRAAASGVPPG
                     WRLAAIYSLAAAYAGAAGALMAQTTQFVSLDALAFHRSADLMLVLIIGGTGYLYGGII
                     GAIAFKFLQDWIAALTPQYWQFWIGLLLVVFVLGGREVIYGGVGAVARRIRSLAPGGR
                     RP"
     gene            complement(51104..51961)
                     /locus_tag="PK812_00615"
     CDS             complement(51104..51961)
                     /locus_tag="PK812_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRJ68082.1"
                     /translation="MLTLLFDGIAYGMVLFVLACGLSVTMGLMNFVNLAHGAFAMAGG
                     YVTVVLMQRLGVPFLWTLPAAFLISALAGVILERLLYRPMYAKSHLDQVLFTIGLVFM
                     AVAATDYVMGSMQQIIRLPPWLQGRFEVAGIAVGRYRLFVIILCGLLVLALQYAFTRT
                     RFGSRLRAAVDDARVARGLGIPVSQLFAVTFAIGSGLAGLGGALGIELMGLDPTFPLK
                     FMIYFLIVVTVGGTSTLTGPFLAAMLLGVADVAGKYYIPWAGAFIIYAIMVVILIVKP
                     EGLFARSRT"
     gene            complement(52005..53129)
                     /locus_tag="PK812_00620"
     CDS             complement(52005..53129)
                     /locus_tag="PK812_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019997214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRJ68083.1"
                     /translation="MMPSIARAQGEPIKIGLVLPLTGPFTSTGKQIAAAANLYMAQKG
                     ASVAGRKVELIIKDDTGVPDVGRRLSQELIVNDKVTAIGGFGLTPIALATAPVATQGK
                     TPMVVMAAATSIITERSPFIVRTSQVVPQISGPFGTWIAQQGIKRIVTMVSDFAPGHD
                     VEASFTATFKEAGGQTEAMRIPLQNPDFSPFLQRAADARPEAILIFVPSGQGSTFMKQ
                     FAERGLDKSGIRLIGTGDVTDDDILPAMGDAALGVTTAHHYSAAHPSATNKAYVEAFQ
                     RANPGMRPNFMSVGGYDGMHALYEGLKKTNGQGGEALVEAMKGLSWESPRGPMSIDPE
                     TRDIIQNVYIRKVERVNGELHNVEFHTIPNVKDPAKAAKK"
     gene            complement(53229..53918)
                     /locus_tag="PK812_00625"
     CDS             complement(53229..53918)
                     /locus_tag="PK812_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010684191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047443 -
                     4-hydroxy-4-methyl-2-oxoglutarate aldolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxy-4-hydroxy-2-oxoadipate
                     aldolase/oxaloacetate decarboxylase"
                     /protein_id="HRJ68084.1"
                     /translation="MTPTVITGVPRPDAAAVETLARLGVSTTHEAMGRIGLLKPYMRP
                     IYPGACIAGAAVTILAQPGDNWMIHVAIEQVQPGDVLVVGCTADNTDGMFGDLLATSA
                     KARGARGLIIDAGVRDVKPLTEMGFPVWSRAISAKGTVKATLGSVNVPIVCAGALVQA
                     GDVVIADDDGVVVVPRLDAAKVAAAGLAREANEVDKRRRLAAGELGLDMYGMREGLAK
                     AGLVYRGWNNT"
     gene            complement(53915..54631)
                     /locus_tag="PK812_00630"
     CDS             complement(53915..54631)
                     /locus_tag="PK812_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007820776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HRJ68085.1"
                     /translation="MTKTGLVITAHPGDFVWRAGGAIALHARRGMRIKILCLSYGERG
                     ESQFAWKKAGATLAEVKAGRKDEAERAAAVLGAEIEFMDAGDYPLRTNQAMLDRAIDI
                     FHDMNPSFVLTHSLEDPYNVDHPEATRFAQEARIIAQAAGHNPDPNRAYAAPPVFLFE
                     PHQPEQCNFKPNVILNIDEVWETKRKAFEVLAAQKHLWDYYTRVALNRGMQGGRNSGK
                     AMTYGEAYQRLFPEALETLA"
     gene            complement(54644..55738)
                     /locus_tag="PK812_00635"
     CDS             complement(54644..55738)
                     /locus_tag="PK812_00635"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010969456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HRJ68086.1"
                     /translation="MALRGVRCMLMRGGTSKGAYFLADDLPADEAERNALLLALMGSP
                     DPRQVDGLGGAHPLTSKVAIVSRSTEPGIDVDFLFAQVGVDRPMVDTTPNCGNILAGV
                     APFAIERGLVRALESKTNVRVRTVNTGTVADLLIETPAGRVNYDGSARIDGAPGTSAP
                     ITVTFLDTEGSVCGSLLPTGHAVDVIDGVPCTLIDNGMPVIVMKAADMGRTGHESREQ
                     LDKDDELKARIESIRLKAGPIMTLGDVSKKAVPKICLVAEPRAGGSMATRSFIPHECH
                     ASIGVFAAVTVATAAALPGSPASTVTRMPEGRERAVVVEHPTGEFTVNLTVGGTPERP
                     VIERAGLLRTARMLFDGTAYAPERRLAAAE"
     gene            complement(55740..>56022)
                     /locus_tag="PK812_00640"
     CDS             complement(55740..>56022)
                     /locus_tag="PK812_00640"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HRJ68087.1"
                     /translation="APDLPTIAESGLPNFEALQWYGLVAPARVPEAIIRRMNAEINAL
                     LGSPEVETTLRNDGAIAAPATPDRFGALIQSEIATWREVIRRAGITDGG"
BASE COUNT        10025 a        17511 c        18305 g        10181 t
ORIGIN      
        1 caggcgcagg ctcccgcgcc tgcacccgtc ggcaactcgc ggcttgccga ggtccagcgc
       61 cgcggaaccg tgcgcatcgg cacgacgggc gacttcaacc cgatgtcgtt ccgcaatccc
      121 ggatcgaacg agtacgtcgg gttcgacatc gaggccatga cccagttcgc agccgacctc
      181 ggcgtgaaga tcgagtgggt ccccacggaa tgggccacga tcgttgccgg catcgccgcg
      241 aaccgcttcg acatcttctc cggcgcctcg gtcagcatgg ggcgggcgaa gacggctgcc
      301 ttcagtgtgc cctatctgga agccggcacg gtgccgctgt cgctcaagac caacgccggt
      361 cggttcaaga cctgggaatc gatcaacgcc gccggggtca aggtcgcggt cagcatggga
      421 accgttttca acgatcaggc caaggcgcat ttcccgcagg cgaccatcgt ggccgtccag
      481 gccccggcca ccgggttcca ggaagtcctg gccggacgcg ccgacgtcac catcacgagc
      541 aatgtcgagg cctcgacgct gatcaagcgc ttcgatcagc tggcgatcaa cgtgcctggc
      601 gcggagatgc gcaacaagcg tccgttcgcc tatgtcatgc cgctggatga cgttagctgg
      661 ctcaactacg tcaacacctg ggtgacgttg aagcgcatcg agggctactt cgcggccctg
      721 gagaagaagt ggctgccttc agcgtgaaaa cgacgatctc gcgcgcggca acggctggcc
      781 tcgttctggc cgctgccctc gcgctgggcg ggtgttccgg cacgacctat tcgtggggtt
      841 ggcatgtcgt gtcgccgctg gatcagcgcg gcctgaacaa cctgatcttt ctcgcgtcgg
      901 gggtctgggc cagcctgctg atcgcgctcg tctccattgt cttttcggtg gggatcggcc
      961 ttgcggttgg catcgcggcg ctctcgaaac agccctggct gcgcaccgtc aatcgccttt
     1021 acgtcgagat cttccgcgca gtgccgctgc tggtcctgct gttgtgggtc tactacggtc
     1081 ttccggtggt cgccggcatc cagttcggtg tcttcgccgc cggcgtcata tcgctggcgc
     1141 tgtccgactc ggctttcgag gccgaaatct tccgcgcggg catccagtcg atcccgaagg
     1201 ggcaatggga agctgggcgc agcattggcc tcacgtcgtg gcaggtcatg cgcctggttg
     1261 tcctgccgca ggcgatccgg cgcattctgc cggcgctcgg caaccagttc gtctacgttc
     1321 tgaagatgtc gtcgctgatc tcggttatcg gctatcagga gctgacgcgc cgcgccaacg
     1381 agctgacttt gatcgagtac cgtccgctgg aaatctacac gctgctcgtg ctcgagtatc
     1441 tggcgctcat ccttgtggtg tcatggcttg tccgcaagct ggaacagcgc atggccgcgg
     1501 gcgatcacgg cagctaggtg gctcaggcag cacggcagaa cgcatcgaaa tcggcgctga
     1561 ggtcctgcac ggcaagtttc agcagttcct ccccggccgc gcgcgtagcg agcgccgaat
     1621 gcgagatcac gcgtccgtcc gggaacctgg cccgatagtc ggcggcatcg tgatagttgt
     1681 cgccagcgtg cacgatgagg tcctcgccga ccgttatcgg cggaggccgg tcgaaggggg
     1741 cgatgacgtc gggatgcgtc gccatggtca gtgcgacttc cgaaggcgtg acgtgaaacc
     1801 cctcctgcgc tccgtagagc gatttgcgca gtgcatccgc tgccggcatt tcccaccagc
     1861 tcttgagacg gcattgcacc gaagccaatc cctcaccgcc ggcaagagac cgggcggcat
     1921 ggtactcctg gatgccgcag cgcagcgggg caagatttcc gccgtgacca ttgaggaaat
     1981 acagccgccg gaacccggtg gcggcgagcg aggcgacgat gtcgtggacc attgccgcga
     2041 gggtggaagg gcgtagcgtg accgtgccgg gaaatgccag atggaactgc gacatcccgt
     2101 agctcagggt cggggcgacg acgattccat gctcttcgcc gcagcgccgc gcgatggctt
     2161 gcgcgcacag atgatccgtg ccgatcaggc cgtcggggcc atgctgctcc gtggcgccga
     2221 ccgggatcag gatgccctgc cggcgcgtga ggtagtcggc caccgccggg gaggtcatca
     2281 gttcgaggcg catgatgctt ccgatgcgat gctgtggcag gatcacgcag tgatggcaca
     2341 cacaccgacc catgacgagc ccccatgcac ctcatccaac ttgctgtctc gctttccagt
     2401 ttcaaggtcc gggcagccct gaagctcaag ggcctcgacc tgccgctgga ggacccgccc
     2461 gggggaacct accgctccga agccttccgc gcggtcaatc cggcagggac gattccgacg
     2521 ctggtcgacg gagatttctg gctggccgaa agcgatgcga tcatcgacta cctcgatgac
     2581 gtcggcgcgg gacggccgtt gcggccgacg gagttcaggg cggctgcgcg cgcgcgcatg
     2641 gtctcgcgct gggtcgactt ccggatcgat ccccagctgc gccggctttt cccgcatgtt
     2701 gcgccggatg cacgggacga agccgaggtg cgccgcgtgg atgaggccct ggccatgggc
     2761 ctggaattga tcagccgggg actcgatgcg gaaggaccgt tcgcctgcgg ccgacagccg
     2821 agcgtcgccg actgcgggct tctggcctgc atgatctggc tggacgcgat gaaggcgcca
     2881 ttgtcattgc gcgccgaagc tgacggccgg ctggcgggcg tcattgccgc tcttgaggcc
     2941 gtcccggccc tgacgagcga agcggccgcg taccgcgcac tggctgccga atgggtgcgc
     3001 gcacgcagcc atcgcgcgcg atgacaggcg gccggcaggt tccaacgcac cgcgcgcatc
     3061 cccgcgacga ttttcatctt tccagcccag cccgagccag cgtagtcttc catgaaagcg
     3121 aatgcagcgc tgtgcctggg gccagacgct gcaagataag gggagacgct gatggcgcga
     3181 cgcatcgtcg atatttcggt tccgctggag gccggcattg cttccgatcc gccggggctg
     3241 gaaccgaaga tcgagtacat gacccacaag gagagcattc cgggcatcct ggccttcttt
     3301 cccggtctca ggccggaaca gttgccggat ggagaagggt gggcggcgga gcgctgccag
     3361 atttccacgc acaacggcac gcacatggac gcgccctacc actacgccac gacgcaggac
     3421 ggcgggaaac gcgcgcagcg cattgatgaa attccgctcg actggtgttt gcagcccggt
     3481 gtgaagctgg atttccgcca tcttccgaat ggccatgtcg tgtcggctgc ggagatcgag
     3541 gcggagttga agcgcatcgg gcatacgttg aagccgctcg acatcgtgct gtgcaatacc
     3601 gccgcgggcg cgcgctacgg gcaaccggac tatgtgccga cggggtgcgg tttcgggcgg
     3661 gaggcgacgc tctacctgac ctcgcgcggc gtgcgggtgg ttggcaccga cgcctggtcc
     3721 tgggacgcgc cgtttggcta cacggcccag cgcttcgcga aagagggcga cccctcgatc
     3781 atctgggaag gccacaagtc gggccgcgag atcggctatt gccagatcga gaagctggct
     3841 aacctcgacc agcttccatc gtccgggttt atggtttcgt gcttcccggt caaggtgaag
     3901 gcggcgtcgg gtggctggtg ccgcgccgtg gcgatattcg acgcgtgagg acattggcat
     3961 gaagctcgcg actttcgtcc gcaaggatgg ttctcccgcc gttggcgttg ttgacacgga
     4021 gcgcggccag atcctcgatc tggcgctcgc cgcaaggcag tccggcatgc ccgaacggca
     4081 tttcgcctcc atgctggcgc tgatcgatgc cggcagcgat ggcctggcag aggcgcgtcg
     4141 actggccaag acctggccgg ccgcctacgt ctcgctcaag ggcgcgaaac tgctggcacc
     4201 gttgccagag ccgcgccaga tgcgcgactg catggtcttc gaggagcacg tggccaacgc
     4261 ctacaagcgt gcatcggagc gcaccggccg gccgctcgcc ccaatcccgc aggtctggta
     4321 tgaccaaccc atctactaca aggccaatcg cttcgcggtc tgcgggccgg acatcgacgt
     4381 tacctggccg cgctattcgc aggtcatgga cttcgaactc gaaatggcct gcgtgatcgg
     4441 caagcgcggc aaggacatcc aaccggacca cgcccacgaa cacatcttcg gcttcacgat
     4501 cttcaatgac ctgtcagcgc gcgatgcgca gatggtggaa atggcggcca tgctcgggcc
     4561 tgccaagggc aaggacttcg acaacgcgaa tgtcctcggt ccgtggatcg tcacaccgga
     4621 cgagatcggc gatccgcatg ccctgacgat ggaggcgcgc gtcaacggtg agcgctgggg
     4681 tgggggaacc tcggcggcca tgcatcatcg ttggccggcg atcctggcgc atgtctcgaa
     4741 aggcgagacg atctacgccg gcgaggtgat ctgctcggga acggtcggca ccggttgcgg
     4801 actggaactc ggccggcagt tgcaggacgg cgatgtggtc gagctggaaa tcgagaagat
     4861 cggcgtactc aagacccgcg tgctgcgacc gaagtagggt aggcgctact tcgtcagcgc
     4921 ctcgtagacg ccgtcccagt ccttgggcgg tggcagcgtg cggaacagcg cgatgcgttc
     4981 ggccagcatg gtgagatagc catcaaggcc gaaagaggcg gcccgcgcgc ggaattccgc
     5041 gatgcacgcc tccgcttcgg cccaatcctg tcggcgatac gcggagagcg cccggttgca
     5101 gccggttgcc gccacgaggt agtccggtcg ttccagaagg tccggcccgc cgatcagggc
     5161 acagatccgc tcgggcatcg tcttgccctt cacccggatg agatcgagct cgatgacggc
     5221 gaaccggccg gtcagggcgc tggccgttgc gtctcccagg atgatgctga cgccgtatcc
     5281 cttcgtttgc ccttccagcc tggaagcgag gttgacggtg tcgccgagcg cggaatagtc
     5341 aaaccgacgc gatgagccca tgttgccgac gacgcaatcg ccggtgttga taccgatgcc
     5401 gatactgagg tcgggcaggg cgcgcccgtc acgcgtggcg gatgcctgcg cccgcgcatt
     5461 gacctccgtc atcgcattct gcatggccag cgccgccctt gcggcgtgcg ccgggtgatc
     5521 cggcacatcg agcggcgcgt tccagaacgc catcaccgca tcgcccatgt acttgtcgat
     5581 cgtgccctgt tcggcaacaa tcgcgttggt cagcggcgtc agcagcgtgt tcatcagcgc
     5641 gatcaggccc tgcggatcgt gcttgtagga ttccgacagg ctggtgaacc cgcgcacatc
     5701 cgagaacaga accgtgaggg gccgcctttc gccgccgagg ctcagcttgc tcgggtcctt
     5761 ggccagggcc tcgaccatcc ccggcgcgag gtagtgtgag aaggcagagc ggatttgctg
     5821 acgctgcatt tcctcgcggc ggtagttgac gaaagccagc accagaaacg ctgcgccgct
     5881 ggtgaacagg ggaaacagcg gatcgagcag cagcccatga ttggcgaaca gccaccacga
     5941 tccgccgccg atgatcgccg cgccgacggc gcccaacaag agggtcggca aagcgccgag
     6001 caggggcaag aggctgatca ccagtgcgga acttgtgacc gccgccacga tctcggcgcc
     6061 gatggcgtag tgcgggcgcg tgagctggtg cttgcccagg atcgattcca gcgcctgcgc
     6121 atgaatctcg acgccgggca tgaccggatc gacaggggtc ggtctcaggt cgaacagccc
     6181 gaccgcggag gttccgatca acgcgagatg accggcaagg cggccgggtg gaaccttccc
     6241 ctcgagaaca tccgcggccg agacgaagcg ctgcggcgca tgcggtgtga aatgcagcca
     6301 gagatggccg gtacggtccg tggggatttc aacgccggcg acgacgacgg acttgacgcc
     6361 gacgtcgtcg cgtttgaccg cgaaggcatc ctgtccggtc gcgacacgca gcgtctcgat
     6421 gctgagcgcc ggttgcaggc gcccgccgac aatggcggtc agcggcacac gccgcaccac
     6481 gccatccgga tcgggcagaa tcgagaacag gccgcgcccc gtcgccgcct gttcgagtgc
     6541 cggcacattg gtcagccagg ccggatactg ggcaagccat ggcctagggt cgccaccgat
     6601 cgtggcgacc ggcgtctccg gaaccgcgcc aagatcgcgc tgctcgcccg ccagatgcgt
     6661 gccggactgg ccaacgacga cgcgcgcgcc ctgaaaggcc cgcgccagca cggagtcgtt
     6721 gtcgggaagg ccggcaagtt cggcgcgtgc tgcatcgctg aggcctgcga cggtctttgc
     6781 gtagctctcc ggagacatgc ggtccggctc tgcaaacacg atatcgaagg cgatggcggc
     6841 gacgccctgc gccttgagcc tttcgacgag ttcagcgacc cgcgtgcgcg cccatggcca
     6901 ctggccgacc cgggtcaggc ttttctcgtc gatgtcaatg atggcaaccg gtgccggctt
     6961 gtaggcgcgc ggcttcagct tctggtagtt gtcgaagacg cgatggcgcg caacttcggt
     7021 aatgacggga tcatagatgc gcagccacac aagtccggcc acgaccagaa acgcgagcat
     7081 gcgccctgat gtgaggattc tcgcgatacg gcggacaaat ggcaccccgg caccatgcct
     7141 cagaggaact gggcgatgat ttcgcctcgg aacgacgatg agctgtcgtc aagcagaacg
     7201 gcgatgttgt cgttcgtccc ggttgcgacg ccgttcaggt cgccgatcca ctgccaggca
     7261 tagcctgatc ccgttccgtc caggtcgact tcgagactgc cgttgagcac gcgtacgaaa
     7321 tcgtccagcg aaccgcttcc gatcgcgccg gcggcgaccg gaacccagat cgcatcctcg
     7381 ccttcggtgt agtccagcgc cgacgcgccg ttcatcgtat ccatctggaa cacgaagacg
     7441 tcgcgccccg cgccgccggt catttcctcg gcgaaggcca actcggtgat gaagatgtcg
     7501 tcgccggtgt tgccagccat gacggtgagg tcgtcgctgt cctggaacgt catgtcggca
     7561 tagccgctga gcgcgccatg ggcatcggac accctgtagg tgatgatttc cggccccgtg
     7621 atcaacggct gttgcacaaa ctcaagatag cccggcccaa aggtcggatt ggccggcgtg
     7681 ctggtcgccg aattcaggaa caccgagttc gcgtcgtcga cgtccgtgtc gttgaactgg
     7741 atggcggaat aggggatctg gatggttgtg ccgatatagc tgttgaagcc gaacacggtt
     7801 tcgccgttgg ccaccggcgc gtcgttcacc gccgtaacca caacgttcat cacggcgctg
     7861 gccgactctg tatccgtgac tgtgccagtc gtcagaacgg cggtatcggt gacattagcc
     7921 gtgactgtga gggtgaacgt gccgttccag ttcgccggcg gggtcatctc cagcgcgatg
     7981 gttgaaagcg cgttcacatc gccgccgtcg ataatccagc gcgcggaatt gcccggatcg
     8041 accgcgccct tgttgaacgt cgcaccaacg ggaaagccgg tcagcgccac ttcgatggtt
     8101 tccgagccgt ccgtgtccgg cgtcgagaat tgcatcgtgc cgaggttgat ggcggtgtcc
     8161 tcggggccgg tgggcgtgag ggcgtagagg cgttgtgcga cttcggtgct gccggaaatc
     8221 acggtatccc acgggatgac gatggtcccg ttgttcaatt gggcgagcga ggtctcattc
     8281 aggttggggg actgatagtc gagcgttgtt gtattgacga cgaactcaga gccaaccatg
     8341 acaccgtttg aactgaaccg ctggcctatt acgccatagt cgctaccatc ctgtccgaaa
     8401 gatgcccatg tcacgaggaa tccgccgtcc gctagcgccg tcactgacgg ggccatttga
     8461 tcattcacgg tcgtcgtgtt gatagcaaat tcagaagtga ttgccgttcc ggaggcattc
     8521 aaaatttttc caaatatgcc gttctggtca ctatcttgac tctggctttg ccaaaccacg
     8581 atgatgtttg tgctgtttaa ttccgccgca gtcaccttca tctggtggcc ggcgaaggtc
     8641 gagttgatgt taatttctcc tgttagtgcc gttggagtag aactataaaa acgtccaaac
     8701 acatcgaaag aattagcgcc ggcttgattg taagactccc agaataccgc aaagcctcca
     8761 ccggtctgcg caacaacaac cggattctgc tgctctgcgg cggtgtaggt gttaactggc
     8821 aactcaccgt ttacaattgc gccggacgcg ttaaatttct gaacgtagac gccagtcagg
     8881 ctgccatcgt tgttttctga gttccatgcc acgacaaacc cgcccccgtc cagcgccgcg
     8941 acggaggtct cggtctgtgc gttggcgtaa gtggtgttta gttggaattc ggcagtgacg
     9001 ggattcccgg ttgaatcaaa gatgcgtccg ataacggcct gtccgcttcc gtcctgtccg
     9061 tctgaagtcc aagtaaggac aaagccgccg cccggctgtg cggcgaccga aggccggtac
     9121 tgatcgccgg tcgtatagga atttgcgatg atctcgttgg tcagggcgtt accagaggca
     9181 tcatagattc ggaaaattac agccgttgcc gagccgtcta ggccgctgcc gtcctgccag
     9241 gcgatgacat agccgcctcc ttcgagcgca gcgactgcgg aataatactg cgaatgcgtg
     9301 gttgtggtgt tcaccaaacc tgccgcgcca accgcaagtg gcgtcggcgc gagcgcatcc
     9361 agcacaatcg tcggcgcgtc ggccaccggg gcgatgtcga tcgtgatgtt cgcgaatgac
     9421 gtaacgccgg ccccgtcccg cagcgtgtag ccgaactggc cctgcccgga gaaatgcgcg
     9481 gtcgggttga tgttggcgcc cccgccggaa aaggcgatgg agccgccgga aatcgcggtc
     9541 aggctataga gctgcttggt gtcgttgatg tccgggtcga tgtcgttggc gaagagcgtc
     9601 gtccgcagga tggtcgcgag cgtgtcctcg gtgccggaaa cgatgtcgtt caccgccgtc
     9661 ggcgcgtcgt tcaggccgtc gatcgcgata acgacgttcg acgcagccgt agcgccgtga
     9721 ttgtccgaca cggtcaggcc gaaggaggcg aggagctgct cgcccggcaa caggccctgg
     9781 accgaagcgg acaggttgtt gagcgtgaag acccagttgc cggcatcgtt gatcgacagc
     9841 gtgcccagcg cgttcgtcgc ggagccgctg cccgtgaagg cgacgccatt gacatgcgtc
     9901 acgctcaggg tgtccgggat atcgatgtcg ctgtcgttga tgagcgcgct gccggacgta
     9961 ctcacgtcgt cttcaatgat gctgccgctg tcatcggccg ccaccggcgc gtcattcgcg
    10021 ccattgatgg tgatgtcgag gtaggcgttg gtgatgccgc cgttgccgtc agcaaggtag
    10081 tagtaaaacg tctcggtgac gaactggccg acagcgagga tcgacaggtc ccagatgttc
    10141 ttcggcgtcg agacgtaaga gccgttcgcg tagaaatcgg tgatggaata gtagccggtg
    10201 atggtgatcg gcgtcacgcc gccggagggc atgggcgtcg tgtccacggc cgtgaccagc
    10261 agaacgtcgc cgttgtcata gtcgacatcg ttggcgagca ggttgcctgt ggccgccacg
    10321 gcggcgccga tgccggcggc tgtcgacgcg ttcgtgtcgt cgatgccgat gggcatgtcg
    10381 ttctggccct gcagcgcgac ggtcaactcg ccggtggtct ggccgccttc cgggtccgcg
    10441 acgacgtagt agaaggtttc gctgccgaca tcgcgcacgc tcagcgcacg gtttgccggc
    10501 ccgctgttgg gcacgaagac gaagtccccg gtggtgcttt gcaccgtcag ggtcccacgg
    10561 atgccgtgca cgacgatggg gctgttcgga acgatgccat tgaccgaggc aatccggaac
    10621 ggtccgaggg aatcgatgtc ggtgtcgttg gacagcagat tgccgtaggc attgacgccg
    10681 ggatcgtctc ccggcgctgt ccccgcgaaa tagtcactgc gcgcgacagg gttgtcgttc
    10741 gaacccgtga tgaccagttg cccgctggtc gaggcgacat aggtgccgtt cgaggcactg
    10801 taggagatcg acagcgtcag ggactggccg ttgttcagat atttgaactg cgccggatca
    10861 atcgcgagcg tcgtgccgaa caggaacgcc gcgaccgacc cgccgttgga ggcggtgacc
    10921 tgtatctgag atattgtcag tccgaattgg ccctgcgcca gatcgaaaaa cacgatctgg
    10981 ccgttctccg tcaccgatcc gccgttcagg gggttgagca agaacggccc cccgatcgcg
    11041 tccagcacga tggggtcagg cacggtgagg ccgatggaga cgaattcgga tggcttgtcc
    11101 gccccttccg catcaacgcc ggcgaactgg acggcggtcg gtgccttcgg cgccttgtcc
    11161 agcgcggcca gatcgatggc ctgccgatcc gagtcggtca ttgcaacatc gatgttcccg
    11221 accagtggat tcagcggcac aagctgcgtt cggtcgtccg gttggggcgc cggctgctgc
    11281 ggatcaatgg ccggcgactg gagcgagctg cgataaaggc tggtgatctg cgcctgcagg
    11341 gtctggctcg tcgccacctc ggccgtcgtg tacggccggt cgagcggtac cgagccggcg
    11401 gacgccagga ccacggcccg gccggattcc gaaacggagc ggatcagccg gcccgtgagc
    11461 acctcaagga ggtcgtaggt gccaatccgg ttgctgtccg gatcggggat gatcgagaag
    11521 accgtcaagc cgttggcagc gttgatgttg accaccggcg tcgtgccgcg gatgcccatg
    11581 gtggcgaccg gcgtggtgat cttcatgtcg ccgttcgtcg cggtctggcc ggcgacgaaa
    11641 acgaacgtgc cctggatgag gttcgcggcc atcgaattgt ttgagccgcc aggattgaat
    11701 acgagttcgt tcaggaccat ccgcgcattg gcggagagcg agaagacggt gccgtcaata
    11761 aacttgatgc caagattgga gctggcgccc tgcgtggaaa cgacgtcgcc cttgaagacc
    11821 ggatcgccca ccagcaggcg cagcgaagag ccgccgcgct gcacgttggc attgccactc
    11881 agcacatcga cgcgcccgat cggggcagcc cctgccggcg ctccggcctg agccaccatg
    11941 tcggctaggg tttcgcccgc gagggaccgc accgcctccg gcagcatgat cgcgccatcg
    12001 ggtgaggcga gcaggggcgt ttcggccgca gcgaaatagc cgcgcaccag gaagcgttcg
    12061 ccgctcggtc cggtgatcag gagatgctgg tcgacgcgtt cgaactggcc ctgaaacagg
    12121 gcttcagcac tggccaagac ggctgtgccg ccgcgagcga caatgggtag ggcgtcgagg
    12181 ttgtcgccac cgagcttgta caaacaatcg ctccggaaaa atacggctca ggccccggga
    12241 ggtttgtgcc tcagactgct tgcaaagtcc acatcttgtt aacaaagctt gcgcaatcgt
    12301 taacatccag agctggcgcg tcagctccgc ccttcctcga cggcgtggca ggccaccact
    12361 gcggctccgg ctgagcgagg caagggtact tcccgtttgc agcgttcgtt cgccagcgga
    12421 cagcggggat ggaaggtgca gccggaaggt gggttgatcg ggttggcgat ctcaccctgt
    12481 accgcaaccc ggtccttgcc gatgccgtgc aggtcgggca cggcggcgag caacagacgc
    12541 gtgtaggggt gccgaggcgt tcggaacagg tccttggagg gggcgacttc agccagacgc
    12601 ccaaggtaca gcacgccgac ccgcgtcgcc atgtgcctga tcacggcgag gttgtgcgag
    12661 atcagcaggt aggtgaggcc gaactgctcc tgcagatcgc gcatcaggtt gagaacctgc
    12721 gcctgaaccg agacgtcaag cgctgacgtt ggctcgtcgc acacgatgaa ttccggtcgc
    12781 gaggccaggg cgcgcgcaat ggcgatgcgc tggcgttgtc cgccggaaaa ctcatgcggg
    12841 tacttctgtc cgtcgagcgg atcgagcttc accagccgca acaactcccc gacgcgtgct
    12901 tcgatgtctt gcggatcact ggcgatgccg aaagcgcgga tcggctcgga aatgatcttg
    12961 tcgacccgcc agcgcggatt gagcgaggcg taggggtcct ggaaaatcat cgtcaggcgc
    13021 cggcgctcgc gcgccatgtc ggcggcccgg gccgggtctg ccacatcgat gccatcgaac
    13081 aggactttgc ccgaggtgat cggatagagc cccacgatca tgcgggcgat ggtggacttg
    13141 ccggagccgg attcgcccac cagcgcgaac gtctcgccct tgttgattga gaacgagacg
    13201 tcggtcacag ccttgagcgt cttcttgcct tcgccggcca gcgtgcgctc aagccagggc
    13261 ttcgagacgt cgaatacctt cgtgaggtcc gaaacctcga gcagggcgcg cgtggagctc
    13321 atgcctgccc cctcttggtg tcatagagcc agcatgcgac ctttccggcg ccggcgggga
    13381 tcgggtcagg acgctccatc ctgcatcgct cgaagacttt ttcgcagcga gggttgaagg
    13441 cgcagccacg aggaatggcc gtcaaccttg gcatcgaacc cggaatctgc gccagacgct
    13501 cgacttcggt gtcgagcgcc gggatcgcgc ccatgagacc caccgtatag gggtgcttcg
    13561 gcgacaggat cacgtcgcgt accggtccga tctccgcgat ccggcccgca tacatcaccg
    13621 ccacgcggtc agcggtctcg gcgatgacgc ccatgtcgtg cgtgacgaga atgaccgccg
    13681 tgccgcgttc gcgacaaagg cgcttgagca gtgaaatgat ctgagcctga accgagacgt
    13741 ccagcgccgt cgtcggctcg tcggcgatca ccagatcggg ttccgcggca agggcaagcg
    13801 cgatcaccac gcgttggcgc atgccgcccg aaaactggtg cgggtaggcg tcgagccggt
    13861 cggcggcagc ggggatgccg acatcgttca gaagctcaag cgcccgggcg cgcgcggcgc
    13921 tgtcgctcat ctcgcgatgc gtctgtatcg tttccgtaag ctgctggccg accgtataga
    13981 gcggattgag cgatgtcagc ggatcctgaa agaccatgcc gatggagcga ccgcggacgc
    14041 ggcgcatctc ctcggccggc aggttgtcga tgcggcgccc gccaagcacg acctcgccgg
    14101 cggcgatcct tcccggcggc tccagcagac cgatgatggc ggccccggtg agggacttgc
    14161 cggcgcccga ctcgccgacc acccccagca cctcgccctt ggacacgtca aaggagacat
    14221 cgtcgacggc gacaagcgtg ccccgtctgg tcggaaactc gacgcgtagg tgtttcacct
    14281 ggagcagggg atcgctcatc aacgcagcct cggattcaat gcgtcgcgca gccagtcgcc
    14341 cagcatgttg atggcaagga cgagaatggc cagcgcggcg ccaggaaata cggtgatcca
    14401 ccattcgccc gaaaacagga agcgcgagcc gatctggatc atcgcgccga gcgaaggctc
    14461 tgtaggggga aagccaacgc cgaggaacga cagggtcgct tcgctcagga tggccagccc
    14521 gaggttgatc gtggcgatca ccagcaccgg tccgagcaca ttgggcagca cgtgacggat
    14581 catgatcgtg aaggccggca gcccgatcac gcgcgccgcc tggacatact cgcggttctt
    14641 ttcgacgatg gtcgagccgc gcacggtgcg cgcgtactgc acccagaagg ccagggtcag
    14701 cgcgatcacg aggattagga aggctgagtt ctcgcgcttg atatgcggaa acaggccccg
    14761 catggtgcca tcgacgaaca gcgcgatgag gattgcggga aaggcgagtt gtacgtcggc
    14821 gacgcgcatg atgaagctat cgaggcgccc gccaatgtag ccggccgtca gcccgagcag
    14881 gatgcccagc accgccgaca gccccaccgc gacgaacccg acgccgagcg aaatgcgcaa
    14941 gccataaagg atggtggaaa ggacgcagcg cccctgatcg tccgtgccga ggggaaactg
    15001 cgcgctgccg tccttgaccc atgccggtgg catgttcgag ttgagcagcg acaattgccg
    15061 caggtcataa gggtcgttcg gcgctatcca gggcgccagc agggcgccga caaagaagat
    15121 cagcgtcacc agcgccgcaa gcatcgtcac cttcgaccgc atgaacgaat gcgcgatgtc
    15181 ggactgccag aaccggtttc cggacgccgg tgccggcgtg ggaagagagg cttctgtatg
    15241 ctgcatcagt gcgccgcctt cactgcgccg cgaatgcgcg ggtcgaccgc gaaatacaat
    15301 aggtcgacaa taagattgat caccacgaac agcaggccga tcagcatcag gtaggccgcc
    15361 atgatcggaa tatcgacggt ctgaacagcg cgcaggaaca gcaggcccat gccgggccac
    15421 tggaacacgg tttcggtgat caccgcaaag gcgattatcg agccaaattg caggcccgtg
    15481 atggtaacga ccggcaccag cgtgtttttc agcgcgtgcc cgaaatgcac ggcgcggttc
    15541 gtcaggccac gcgccctggc gaacttaatg aagtccatgc gcaggatttc cagcatttca
    15601 gcgcgcacca gacgcatgat cagggtcatc tgaaacaggc cgagcgtgaa ggccggcatg
    15661 atgatggcgg accagcctga cttcgtcagc agacccgtgg tccagattcc caggtcgaca
    15721 acctgaccgc gcccgaacga gggaagaacc ttcaactgaa ccgcgaagaa gaagatcagc
    15781 aggatgccga tgaagaacgt cggcagcgag atgcccagca gcgaaaccgc aagcagcacc
    15841 ttggaaagcc agttgtcgcg atgaaggccg gtatagacgc ccatgggaat gccgatgagc
    15901 agcgccagca gcatcgccat catcgccagt tcaaacgtcg ccggcagccg ttccaggatg
    15961 agctgcataa ccggctgctt gaattggtag gacatcccga actcgaaccg cgcggcccgg
    16021 ccgatatagc gggcgaactg cacgacgatc gggtcgttca gcccaaggtc ctgcttgagc
    16081 ttggcgcgct cctctaccgt cgcgtcctga ccaaccatct ggttgaccgg atcgccgacg
    16141 aactggaaca gcgtgaacgc cagcacgccc acggcgagca tgacgtacag cgactgaacg
    16201 agacggcgga ggatgaacgc gaacatggct gaagtcccgc gacccggagc aacttccgct
    16261 ccgggtcgta ggtcaggccg tcactgcatc ttcacagtgt agagcatgat catgttgtcg
    16321 gcgcgctggt cgatcgatac cttcttgctc atgccccagg ccagcgcctg ctgatgcagg
    16381 ggaacgtggc cgacctcgtt gtgaatgatc tcgaaggctt ccttgatcat caggttgcgc
    16441 ttcgcgatgt cattctcgac gaggatcttg ttggacagtt cgtccacctt ggcattgcag
    16501 tagccgccga cattggcgac gccgataccg cctgaaccct tgtcgtcacg gcactggtgc
    16561 aactgctcca gcacgttgtg cgggtcattg gtgccgacgg tccagcccag caggtagaag
    16621 ttggtgttgt agccgcccgg cgccaggatc ttggcgaaat actgggcacg cggctggatg
    16681 atcggctcca ctttcacgcc aacgcgcgcc agcatggcga tgacagcctg acagatcgcc
    16741 gcgtcgttca cgtagcggtc gttcgggcag tccatgccga cggtgaagcg gttcccctgg
    16801 gctccgccca cgcgcgggta gccggcctcg tcaagcagct tctcggcggc cgccgcgtcg
    16861 aacgggtggc gcttgaagtc cttggcgtag cttgggaaca gcgagggatc gagcatcagg
    16921 gcagagggga cggcctggcc gcgcatcacg cgcgacacga tcagattgac gtcgatggcc
    16981 tgatacaccg ccttgcgaac gcgcgcgtcc ttgaacgggt tcttgtcgcc cggcacctgc
    17041 gccggcgcga gcagcttgtc gcggcgctga tcaaagccga ggtagatcgt gcgtgtttcg
    17101 ccgccggcca tcatcttggt cgtggcgttc gagttgacac gttcctgatc ctgcacagga
    17161 accggatcga cccaatccaa ctcgccggac aacagggcgg cgacgcgcgt cgcatcattg
    17221 gcgatcggtg tgaagatcac cctgtccagg ttgtgcttct tgttcttgtc gttccaccag
    17281 tccgggtttt tgaccagttc ggtcttcacg ccggtctggt gtgtggcgac cttgaacggg
    17341 ccggtgccat tggcgttgag ggtggagaag cccggagtct gcgcccgcga ggagatcggc
    17401 tcgacggcat tgttcttctc ggcccaggac ttggacatga tgtaccaggt gtcccagctg
    17461 tagtgcagga tggggttcgg tcccggcatc gtcacgtcga cggtgtggtc gtccaccttg
    17521 gtgaagacag catccttcgg cacgcgcgcc ttgaggtccg aatcctgctt gcgcacgcgc
    17581 tctgcggaga aaacgacgtc atcggcggta aagggttcgc cgccatggaa cttcacgccc
    17641 ttgcgcaggt ggaagcgcca cttgttcggc gcgagcaatt cccagcgctc ggcaagaccg
    17701 ggaatgattt ccagcttctc gttgcgcgcg gtgaggccct cgaagatgtt gccctgcatc
    17761 gccagggtga aggtctcatt gatggtgtag ggatcgagcg tgttcagatt gccctggaaa
    17821 gccatcttga acgtgttctg cgccatggcc tgcggcgcga acagcgctgc cgccatgacc
    17881 gaagcgagcg cgagtgtctt gacgcgtcgg aacatatctt tctctcccgt tcccggaact
    17941 gccggggcgc ctgagcgcaa tctaacaaac ttcccggtcc gtcggatcgc caggcgagcc
    18001 agccccgggc caaattctcc tgacggattc tctccccccg tcgggacgat ggcctgacgc
    18061 tagccattcg tcgcacgcgg ggcaaccgct ttatgccgat acgccacgct ttcgacgcat
    18121 atgcgcggct gggggcctat gaggccttgc cgcctgccag ccggatcgcc tctcgcaggg
    18181 tctcacggtc accgatgtga ttggccagca gcaggatgag ccgggcgttc atcatttccg
    18241 cctgcgcttc cgacatgtcg cgctgggaat cgatcagttc ctggtagaaa tcgtcatggt
    18301 ccggaatgtt cgggcgagtg ttcagcggca cgacaatcct ccctcaggca gcgatcgcgc
    18361 gcgcgagcgc ggctcggatc ttgccaatgt cgaacgagcg ccagcgggca gcgacatgct
    18421 ggtccgggcg gatgagatat gtcgtgccgg gtctggcatc gtagcggcgc gaaagcaggc
    18481 cctcggaatc ggcgaagtcg cggccgacaa ccagaacttt cgccgagacg ccaccatgcg
    18541 ccacctcttc gaccggcggt gtctcgccga agagcaggac gccaaaacca tcgccgatgc
    18601 gattcaggaa ccagccatcc ttgcccgctg agcgcacggg cgcatcgtcg cagttcgtgc
    18661 ccggcttcat gcgaccggag aaaggctcgc tgtccggcgt gttaagcgag gacttgaggt
    18721 atggcgtcgg ggacgacaac cggccactgt tcaccagcgg tcgggcgaag gcctgggatt
    18781 cggccagatc gagcacggca tcccgaaagc gccggctcgt cggacttttc ggcgtgatga
    18841 aatccgttga ccgtgtcgaa ttcagcagat tgtcgtcggc ggcgaatgcg cgctcttcgt
    18901 ggtagctgtc gatcagctgt tccggcgcat cgcccgccag cacgagcttc agtttccacg
    18961 cgagattgtc ggtgtcctgg aatccggtgt ttgcgccacg ggcgccgaac ggcgaaacct
    19021 gatgggcggc atcgcccgcg aacagcacgc gaccgtagcg gaagttgtcg atgcggcggc
    19081 aagcgaactg gtagatcgac acccactcaa gttcgaaatc gcgctcgtcg ccgagcatgg
    19141 ccttgatacg cgggattacc ctttccggtt tcttttcttc caccgggtcc gcatcccaac
    19201 caagctggaa gtccagtcgc cagacatcgt ccgactgctt gtgcaggagc gccgactgat
    19261 tgcgatggaa cggcggatcg aaccagaacc accgctccgc cgggaattcg gccttcatga
    19321 cgatgtcggc gatcaggaac ctgtcctgga agaaatggcc cgtgaactgc gctccgacca
    19381 tcgatcgcag ggtggaattg gcgccatcgc aggcgatcac ccagtccgct tcggtcatga
    19441 acacgccgtc cggcgtctcg acggtgagca cggcgtgatc gtcggcctgc ccgagaccgg
    19501 taaccttgtg gttccagcgc aggtcgacca gcggcgaggc catgatggcc tccaccatgt
    19561 actcttcgag atagtactgc tgaaggttta tcatggccgg catcttgtgg ccggcctccg
    19621 gaagaagatc gaactggtag gaaagccggt cgcggaagaa caccttccca accttccagc
    19681 tcacgccttt cgcgacgcac cgttcgccga cgccgatccg gtcgagaatc tctagcggcc
    19741 gcttggcgta gcagacggca cgcgacccga cgctgaccgt attgttgtcg tccagaaccg
    19801 tgcagcgaat gccgcgcgac gcaagatcca gagccatcgc caggcctatc ggccccgcgc
    19861 cgacaacgat caccgggtgt cgttgcggcc gccccgactt ctgttcgtcc gactggacat
    19921 aggggaattg cgggaactga taggtcttga gcatgtctcc cccgggggtt tgacgccgca
    19981 tcctcggctt ctcaggcact tcactagggt agggcgacgc tccgcgccac ccgccatctc
    20041 agtgtgacgc cttgaattcc ttctcgtgca gccatgcggt gtggcgcggc ggcttcttgg
    20101 tgaccgccca ttcatcaatc atggcatggg cgacgtcgcg catgcgcttc atctcctcgg
    20161 atgtcgtggt ccagcaggcg acctcaaggc gatggccgga cgggtcaaag aagtagatgg
    20221 actggaagat cgaatggttg gtcggcccga cgacatccag accggcggcg agcgcgcgct
    20281 cgcggatggc gttcagcgtc gccatgtcct tcacctggaa ggcgatgtgc tgggtccatt
    20341 ccggcgtatt ggggtcgcgt cccatcgggg gcgagttcgg caactcgaaa aaggcaagga
    20401 tgttgccgtt tcccgcatct aggaagatgt gcatgtaagg gtccggcgcc ttggtggagg
    20461 gcaccttgtc ctcggcgatg gcgcccagca ggtccatgtc gagcaccttc ttgtagaact
    20521 cgaccgaagc cttggcgtcc ttgcagcgat aggcgacgtg gtggattttc tcaacgagca
    20581 tggcgctcct cctgtgtccg cgttattagt aacatgcgtt actaataacg caagcgccga
    20641 caggccttcc acgaaaactt ttgcatcttt tatgggaatg gattgggtcg atccagggta
    20701 ggtgcatttt tcctggcgcc ccaacgatcc catgtgttac taataatggt aacacatggg
    20761 ggtatcatga gttcgggcgg cggcgacggg actctcgacc tgacacggtt ttttccctac
    20821 cgtctggcga tcctcgcaga ggcggtctcg cagacgatgg cgcagctcta cgcggatcgc
    20881 tttgatctga cgcggcagga atggcgaatc gtcgccgcgc tcggcaatcg caagcgtatg
    20941 gcggggcgtg atgtcggccg tttgtccgcg ctggacaaga tgcaagtcag ccgggcgctt
    21001 gcgcggctgg aagggcgtgg catgatcgac cgcgcgccgg atcccgatga ccgccgcaac
    21061 caggtgctgt cactgacggc ggaggggcgg gcgctctacc agaagatcgt gccgctggtg
    21121 caggcgcggg aggaatacat tctgtccgca ttgtccccag aagaggttcg caccttgaat
    21181 gcgctgatga cgcgggtgca ggcgcgcgca gaggagatgt tgcgcagggg ataggcgtgg
    21241 cagtccaggc gcgacacaaa aaaggcgggc atgacgcccg ccttttcaat ggtgatgcgt
    21301 ggagcgtgct cacttcacgc gatgcacttc cacccacttg ccgccctgga cctgcgagat
    21361 gatgatgtcc ttggcgccct cgtgatcctt ctcggagaag gcgatctggg cgccggtgat
    21421 ggcatcggtg tacttcaggc tttccatgcc cttcttgaac gtatccggcg tcagcgtctt
    21481 gcctgcggcc tggagcgcgc gaacgaaggt ctcggcggcc gaacgcccga gcagggcgcc
    21541 ggtgccggca tcttcattgt aggcagcctt gtagccatcg gcccaggcct tcacttccgg
    21601 aacattcatg cgggcaacgt ggtcggccca gccggcggcg gcgtacagcc cttcagtcac
    21661 gccgcccggc accgctgcca tgaccgtgtt gaagccggcg gaggagccca cgagcttggc
    21721 gttccagccc atgcgcttga gcgttcccga gacagtgatc gcctggcgca cgccgagcgc
    21781 atgcacgatg acttcacagc cggcgtcctt gtgcttggag agagggccga cgaaatcgcc
    21841 ctcgtccggc ttgtgagtgg tttcgccggt aagcgtgagg ttcattttct tgattgtgtc
    21901 gacggcgccc tgctggatct ccaggccgaa gtcggacgga atatacatca ggcagtactt
    21961 ggtcgccttg tccttctcgt tgaagtactt aaccgcgccg acggtctggt catagtagga
    22021 tgaaaagccg gtgaacttgt agtcgttcgg cgggtcgagc atctggcgcg ccgaggtgag
    22081 cggcgcgacg ttcgggatct tcttctcgtc ctgcagcttg aaggcggcga tgttcatcgg
    22141 cgtgccgagc gacagcagca tggcgaaaac cttgtcgctg ttgatcagct tgttgatcgc
    22201 ctgtgtcgcc ttcggcatct ggtaggtgtg atcttcgaca atgaagcgaa tccggcggcc
    22261 gtgcacgccg cccttgtcat tgacttcctt gaagtagagc tgggccgcct tcacggccgg
    22321 gctgccgaac gcagcgaagg ggccagacag gtcattgtgc gagccgacca gcacttccga
    22381 atctgtcacg ccctgcacct gcgccttcgc gccggagatg cccagcgcca tggcggcaac
    22441 cgcaagaatg gtggaaagac gtttcatatt ctgcttcctc ctggttttcg aagcccattc
    22501 cgacgggcgt tacacggata ttggggcgga accgtgcccg cttcaaccgc ccttgtacat
    22561 ttgagcgatc aggggcgcat atttcttttc gacaaagctg cgcttgagct tcatcgtcgc
    22621 tgtcagttcg tcgtcctccg ccgtgagcag cacgtcgatg atcctgaaat ccttgatctg
    22681 ctcgacgcgg gcgaactcct tgttcgtgtc gtccacgacc tccttgatga ggtccttcac
    22741 agcctgggcc gcgcagagcg aggcatagtc gttgaacggc acgcggctgt cctgcgcgaa
    22801 cttctcgaca ttctcctgat ccagcatgat caggcaggtc aggtaggcct tgcggtctcc
    22861 gatgaccacc gcatcggaaa tataggggga gaacttcagc ttgttctcga tctcggccgg
    22921 ggtgatgttc ttgccgccgg cggtgatgat gatgtccttg atgcgtccgg tgatgcgcca
    22981 gaagccgccc tcatccaccg ttccgacgtc gccggttcgc aagaatccgt ccggcgtcat
    23041 tgcttcggcc gtcttgtccg gcttgcccca gtatccctgg aacacgccgc cggaacgggt
    23101 ctggatctcg ccttccggtg acaggcggat ctccagcccc ggcatggcgg gtccaaccgt
    23161 accggtacgg ttgttgccaa tgcggtttcc ggtggcgatg gccgtgttct cggtctgccc
    23221 gtacgcttcg aagatcggca cgccgatggc gcgataccag cgcagcaggt caggagagat
    23281 cggcgccgcg ccggaggtgg cgcgccgcac gcgctccatc ccgagcatgc gccggatgtt
    23341 gccaagcacc agccagttcc agagcctgta gctgagcgat tccagcagcg agggcgtcct
    23401 cttttcttcc agcgcccggg catgcgcgtc gccagccttc agcgccagct tgtaggcgag
    23461 cttcccgaaa accgtcgcct cgttgttcat gatcgtgatg cgcgaataga ccttctcgta
    23521 gacgcgcggc acggcggcaa acagcgtcgg cgagacctcc cgcatgttgt cgaacaccgt
    23581 ctcggtgctc tcggcgaagt tcacggtcga accgattccg atcgacagcc aggccgagtt
    23641 gagtcgctcg tagacgtggc acagcggcag gaagcacaac tgttcgtcgt cctgggcggt
    23701 ttccagtccc tgcagggcgg ccgaaatgcc aaacatgacg ttgctctggc tgagcatggc
    23761 gcccttcggc ggtcccgtgg tgcccgacgt gtagatcagc agtgccgtat cgtccggctt
    23821 cgacagggcg atctcgtcgt cgaacagggt cggggtcttc ttcagctcca acgcgccgag
    23881 atcgtagagg tcatcgatga acaggaccgt gtcgtccttg tagtcgtaga gtccctcgcg
    23941 ctcgtagacg atcaccttct ggatggtggg catcttgtcc cgcacctcca gatacttgtc
    24001 gaggtgctca tcgttctcga cgaacaggaa gcggctgccg gaatcgttga cgagataggc
    24061 cagctggctt gccgaatccg tcgtgtacac gccattgaca acggcgccca tgccctgggc
    24121 gccaaggtct gtataaagcc attccttgcg gtcttcggac aggatggaaa tcacgtcgcc
    24181 gcgccgcatg ccgagagcgc gcagcgccag tccgatcaac ctcgcgtgct cccagaaatc
    24241 attccaggag aaggactgcc agtacccgaa atgcttttct cggtgtgccg ttcgcgcgcc
    24301 gaactgcgcg cagcgcttgg aaaacagctt gggcaaagtg tccgcgccat ccacgaaata
    24361 ggggcgctgg agcaggtcgg cattcagact gacgccgttg tgcattttct gcgccggcat
    24421 ggcggcgctc gtggcgtccg ccggggtcac gacaacgttc atcgccaggt cttccgttgt
    24481 ttccagcggc gctggccgcg ggcgccttct tcctgcacgc cgaggtagaa ggtctggatg
    24541 tccttggaat tgcgcaattt ttcagcctcg cccgccatca cgatgcggcc aagctccatc
    24601 acgtaaccat agtcggagat gtcgagcgcc gctttggcgt tctgctcgac cagcatgatc
    24661 gtgaccttct gctccaggtt gagacggcgc acgatgccga agatctcctg ggtcagcagc
    24721 ggcgacaggc cgaggctcgg ctcgtcgagc agcatcaggc gcggctttgc catcaggccg
    24781 cgaccgatcg ccagcatctg ctgctggccg ccggagagcg tgccggcgcg ctgctggtag
    24841 cgttctttca ggatcgggaa atagctgaac accatgtcca ggtcggccgc gatattctgg
    24901 cggtccgggc gggtgaaggc gcccatttgc aggttttccg caaccgacag cagcggaaaa
    24961 atctcgcggc cctcagggac atggatcaca tgcttcttga cgatctggtc cggctccagg
    25021 ccctggatcg cctgaccgtc gagcagaacc tcgccctttt ccggccacat gacgccggag
    25081 atggttttca tcagcgtggt cttgccggcg ccgttcgcgc cgagaacgct caccacctga
    25141 ccttgcgtga catcgaggct cgcgccccgg atggccatga tcggcccata gtagctttcg
    25201 acgttcttga cgcggaggag ggggaactcg ctcatgccgc cgcctccgga gagccgccga
    25261 tataggcgcg gatcacgtct ggatgtgact gtacctcttt cggcgttccc atggccagca
    25321 ccttgccgtc cgccagagcc aggacgcggt cggaaaccgc cgccaccagc ttcatgtcat
    25381 gttcgaccat cagcacggtg atgcccatct gcttcttgat gtcctcgatc cagaaggcga
    25441 ggtcgttggt ctcgtccatg gacaggccgg aggccggttc gtcgagcagc aggagcttgg
    25501 gctccgtcgc cagcgcacgg gccatttcga cgaccttgcg taccccgtag ggcaggcctg
    25561 ccacgtactt gtcgcggtag gcttgcagat cgagaaagtc gatcaccttt tccacggcct
    25621 cgcgatgccg ccgttcctcg cgatgcacga acggcgtgaa ggcaaggtct gaccagaaag
    25681 tcgactgtac atgccggtgg cggcccacca gcaggttctc cagaaccgtc gaacgctcga
    25741 acagttcaat gttctggaag gtgcgggcga tgccgagcga tgggatgtcc gaaggcgcca
    25801 gcttgaggag gctgtggccc tcgaaacgga tgtcgccgga atagggcgtg tagaagcgcg
    25861 agatcaggtt gaagatcgtc gacttgccgg cgccgttcgg gccgaccagc gcaaagacct
    25921 cgccttgctt caccgacatg gtgacatcgt tgacggcggt gatgccgcca aacttcaagg
    25981 tgacgtgttc cagagcgagc atgctcatcg catccgctcc gtcttgagat agctgcggtt
    26041 ccgtctgaac atgtcgcgcc gataaaaggg gaacaactcg aaataggtcc tgatcttgag
    26101 ccagcggcca tagatgccca tcggctcgaa caggatgaag ccgatcagga tcaagccgaa
    26161 gatgccgctc tcgaggccag ggatggcgat cgacccggcg ccgctcagct tgccgatgta
    26221 gtcgcgcagg aaggacagac ccagggggat cgccccgacc acgatggcgc cgaaaaaggc
    26281 gccgtggatc gagcccaggc cgccgaccac gatcatcagc agcagctgga tcgcgatcac
    26341 cagattgaac gtctcgtggt tgaatgcacc cgagaactgg ccaatcagcg cgccggcaag
    26401 gccggtgatg aagcaggaga tgaaaaaggc catggccttg gtcgaggcga tgttcacccc
    26461 catcgcctgc gccgagattt cactgtcccg gatggcggcg aacgagcgcc cgagcggcga
    26521 acgcagcatg ttgcgataga acagcaccac gagcagcgtt gtcgccagca cgaggtaata
    26581 gaatgcgttc ggattggcat agcggtcaaa ggacaggccg aacatcttga tggtctgcga
    26641 ggtcaggccc gtaacgccgc cggtcagcgg ttctgacagg acgatgatgt cctcgatcag
    26701 cacggccagc gccagggtga agatcgcgag atagatgcca tgcagcttga gcgtcgggat
    26761 ggcgacgatc acgccgaaca gcccagccag gaggccggcg accgggaagg agatgaagaa
    26821 cggcaccccc ttcatcatca ggatggtatt ggcgtagcag cccatggcga ggaacgcggt
    26881 gtgcccaagg ctcgcctggc cagcctgtcc tgtcagcacc atcaggccaa ggccggcgat
    26941 ggaccagatc aggatgccgc tgacttcgcc cagcaggaac gaggagatca gcagcggcat
    27001 tgccagcatc agcgctagaa gcagggcgta ccagaaggcc tgggcgccat gtttgaacag
    27061 gcgaatatcc gcgtcgtagg aggtcttgaa gacgtttctc atcgcgtcag accttcttgc
    27121 tgtagacttg agcgaagagg ccgtgcgggc ggaagatcag caccagcagc agcaccgcgt
    27181 agggcgcgat ctgcgagtag ccggccatca cgtaccgcga ggcgaagggc tccacgacgc
    27241 cgaccagcag gccgccgagc agcgcgccgg gcaggctgcc gaagccgccg atgaccgccg
    27301 ccgcgaaggc cttgatgccg agcagaccgg tcgagggatc gatcgagccc ttggtggcaa
    27361 acaggatgcc ggcgattgcc gccgtcccgc cggccagcgc ccaggtcagg ctctgcacct
    27421 gcttgaccgg aatgcccatg taatacgcgg ccagctggtt ctgcgacgcc gcttgcatcg
    27481 caacgcccat ccgcgtgcga ttgaaatagt agtaaagcgc cagcgtgagc agcaccgtta
    27541 ccaccacgat ggcgatttca tcgagaccga ggatgattcc gccgaccttg gcgctctgac
    27601 cggaaatcgg tgtggccagc gcgaccggct catgacccca gatcgcgccg gcaacgaagc
    27661 ggatcacgaa ccccagcgcg atggtcaaga taaccatcgc cgtctgtgat tgcccgaaca
    27721 tgcgcctcag cacgatcatg tcgagcaggt acccgaacat cgccatcgtg gcgatcgcga
    27781 gcggaatgct caggtagaag ggcaactgca ccatcttgcc ctgcgacagg gctataacga
    27841 tgaaagcgcc gagcatcatg aagtcgccct gcgcgaaatt cacctgttcc gtagctttgt
    27901 agatcagcac gaagccgatg gcgatcagcc cataaacaca gccgttcgcc aacccgctca
    27961 ccaagagctg gaaacttgac acccgaattc ctcccgaggc tggtcgctct tgcggcgaaa
    28021 acactgatcc tcagaatcag gatagcaggt tgcctgtgca cggctagccc ccgatggaaa
    28081 aattaggtca cctctaaacg aacggcgcga ccgaacgttt cggcgatgag aatcgcgacg
    28141 tcggctggcc ggaaggctgg cgaaatcgcc tggcgctaga gatagcttgc cgtcgggtgg
    28201 atgaattccc aaggcgaggt ttcgctcccc tgcggcgact gcacctcaat cagcaccggg
    28261 gacttggtgc ggatcacttt cgccagcgtt ttcttcagtg cttccggcga acgaaccgca
    28321 tggaactcga cgccaaagga cttggcgaac agccggaagt caggatttgt gagggtagcc
    28381 ccgatcacgc gattgccgaa cccaagcttc tgatcgcgca acacgttgcc ataggcatgg
    28441 ttgttgaaca ccagcgtgat caatccgagg cgctcctgca ccgcggtcgc cagttcctgc
    28501 gcggcaaaga ggaatccgcc atcacccgtg acggaaatca ccggtctgcc ggggtgcgcc
    28561 gccttggcgc cgagcgcgga cggaaatccg aagcccaacg tgccctgata gccttcggaa
    28621 acgtaggtac gcggcttcag gacctcatag ccgaaatagg tcgtgaagcc gacctgcgag
    28681 agttcaggca cgagcaggcc gtcacgcggc agcacggacc ggatggcgtc gagataggcg
    28741 agttgtggtt ggactttttc ataagccccg cgcgcttgcg ccttggcctc ggcgatgcgc
    28801 ctgcgagcgg ccttgcggtc cttcgggctg gccggggggc gcagccgcat cactgcgtcg
    28861 agcagggcgc gcgtgccctc ggcggaatcc gcgacgatcg gagcatgtac cttgagacgg
    28921 agcatttcct gcggatcgac atcgatgcgg atgatgtggg gcggcgctgc cggctgatcc
    28981 gtgagttgca tcatgccttt ccagcgcatg aacggcagtt ccatgcgcgt gccgatgccg
    29041 actgcggcat ccgtatgcgg ccagagcttg aacgccgcgt aagacatcat gccgagcggc
    29101 ccatcttccg gaacgacgcc tcggccgccg cgaaagccga cgaccggcgc gtccagatag
    29161 tcagccagag ccttgatctc ctgtgcagcc ttctgcgcgc ccgagcccgt gaagatcatc
    29221 gggtgccggg ccgacgccag aatgcgggcg gcttggtcca cggcatcggc ctgcgggggc
    29281 ggcgcgggca ggggcgctgc cgggtcgagc agccggactg gagcggactg ggccatggtg
    29341 tcccagcaca tctcgagcgc gacaggaccc ggtcgtccgg aaagcatctg ccggaaggcc
    29401 tcgttgacca tcgccggaac atcctcgatg cgctctatcc gagccgccca cttgatgaac
    29461 gagcgcaacg tggcgagctg gtccgggatt tcgtgcaggt gaccgcgtcc ccggccgaga
    29521 aacatcgagg gcacttgtcc ggtaaccatc agcagcggcg tgcaggttcc ggcggccgtg
    29581 cagagggcag cgccggtgtt gagcacgccc ggtcccggca ccacagagaa tacgccgggt
    29641 cgccccgtgg accgtgcata accgaaggcc atgtagccgc aggtctgctc gtgccgcgcg
    29701 ctgtaggtcc ggattcggtc ggcgttctgg gccagcccat cgaaaaaggg gtagagctgg
    29761 gcgccgggca ggccgaagac ggtgtcgacg ccgttggcga gcagctggcg aacgatggcc
    29821 atgccgccgg tcatggattc ggtgtggggt gttttggact tgctcatgcg ccaagcatgc
    29881 accatgcgct gcccgccggt ccagcgaggt gtcgggcatc gaaagatcca ggtgccacgc
    29941 gggagtgttc cgaagcgggc gccggcgccc ctccggagtg ccgcgaagac ctcgcaagga
    30001 cgccagcagc gtgggcatgg cggccgctgg ctccaggcag cccagcatgc attttttcac
    30061 ggaatgggat ttcggaagaa aatcgcttgc ggatcacagg ggggcgtagc agatttcccc
    30121 gcgggaggcg tttggaaatg aaccagccgg atggcgggtt gcttgttgtc agcgacgtga
    30181 cgcgcagttt ctacggcgtg cacgcgctga atggcgtcac cctcacggta ccaggccagg
    30241 gaatcacagg cctgatcgga ccgaatggcg ccggcaagac aacgctgttc aactgcattt
    30301 ccggagtcat cccgccggac ggcgggcgca tcgtgttcaa cggacacgac attgccgggt
    30361 tgcgtccgga ccagatcacc catgccgggt tggtgcgcac gtttcagatc gcgcgggggc
    30421 tgccccgcat gtcggtgctc gacaatctca tgctctatgg cgcccatcaa ccgggcgagg
    30481 gggttctcgc ggcgatagcc ggctcgtccg cggcgcgcca ccgtgaacag gagttgctgc
    30541 accgggcggt cgccatcgcg gaacggctca acctgctgcg ggtcatccgc aatccggccg
    30601 ccgccctgtc cggcgggcaa aagaagctgc tggagatcgg ccgggcgctg atgaccgaac
    30661 cgaagttgat cctgcttgat gagccggtgg cgggcgtcaa tcccacactc gcccgcgaaa
    30721 ttggcgaaca cctcaaggtc cttgctgccg aagggctgcc gatcctcctg atcgaacatc
    30781 acatggacat ggtggcccgc ctctgcgacc gggtgattgt gatggcggaa ggccgccatc
    30841 ttgcagaagg caccttcgat gaactggccg acgatccgcg cgtgcaggag gcctacatgg
    30901 gtcgtcgctg gcggagcgcg agctgatggc cgacctggtg gcgcaaggca tcgtcgcggg
    30961 atacggcgcc gcggatgaaa tcctgaaggg tgtcgacttc accatcgcga atggcgaaat
    31021 cgcctgcatc atcgggccga acggcgcggg caagtccacg ctgctgaagg tcattgccgg
    31081 cctgctgatt cccaagtccg gtagcgttgt gctggccggg caggacgtca ccggacgcca
    31141 acccagagac atcgcccgcg cagggctggc ctacgtgcct caggagcaca acatctttcc
    31201 aacgatgacg gtgcgcgaga acctcgaaat ggggggctac gtggagccga acgccgtcga
    31261 tcgacgcatc gaaatggcat tcgaaaggtt ccccgtcctg gccgacaagc ggcgccatgc
    31321 ggcgcgaacg ctgtccggcg gcgagcggca gatgctcgcc atggccatgg ccttgatggt
    31381 cgagccgcgc gtgctgctgc tggacgaacc ctctgccggc ctgtcgccgc tcgcggcgga
    31441 gaagctgttc gacgcgatcg tcgacatcaa caagtccggg ctttccatcg gcatggtgga
    31501 acagaatgca tccgaggcct tggcgatttc gcatcgcggc tacattctgg tggatgggcg
    31561 caacagccgc acggggccgg cccaggcgtt ggctgcggat ccggagatcc gccgcatctt
    31621 tcttggggga tgatcacagc catcggacgg atcgagttcg catgcatcgt tacaacgaca
    31681 ctgtgggaga agtgacatga gtgggtttga tcgcagaacg cttctgaagt tgtccgccgc
    31741 cgccgcggct gccggcgcta tgcccatccg cgcctatgcg cagggcgagc cgatccgcgt
    31801 cggcatcctt acgcctctga ccggcgccgg cggcaacgac gggccgcgca tgctcaaggc
    31861 gatgcaggcc gtgttcagtg aggtcaatgc cgccggcggc gtgctcggtc gcaggatcga
    31921 gacgatcgtc gaggatgacc agaccaatcc ggaagcggcg gtgcgcgccg cccgtaagct
    31981 gatcgaggtc aacaaggtct cggtgatcat gggcacctgg gcctcggcgg tcacggctgc
    32041 cgtcgcgccg gtctgctggg aatccaagac cttcatcctg accgtttcgg gcgccgactc
    32101 gatcacgctg ttgccgcacc aggggttcct ggtgcgcacc cagccgacca gcaagctgca
    32161 ggcaaatgct catgccgagt tcattgctgg catggggcac aagcgcgtgg cggtcatcgg
    32221 cattcaggca ccgttcgcgg tgccgaccaa ggatcacctc gacagcatac tgaagaccaa
    32281 gggcggctcg gtcgtctcgc acgtcatcta cgagaaagac aagccgacct atcgctccga
    32341 gatcgatcag gcgatgaagg aaaagccgga tttcctgtat ctcaacggct atgctcccga
    32401 cacggtggtg gtgctgcgcg atctgttccg cgccaacatc aaccttccga agttctgcca
    32461 gtcctatgcg gtgccgcagg cgacgctcga caccatgccg ccggaagtat ccgaaggcgt
    32521 gatgaccggg cagccctcgg ccgacgtcga gagctctgcc tatcggaagg ccgtcgagcg
    32581 gctcggcgcc aaggagatcg acggctacga agcgcaggcg accgactggg caagccttgc
    32641 cgtgctgacc atggccaagg ccaaggacac cagcggcgtg gcgctcaagg acaacatccg
    32701 caagatcagc cagggttcgg gcgaaaaggt ctacacggcg gttgacggcc tcaaggcgct
    32761 cgccgctggc aaggagatca actacgaggg cgcctccggc ccctgcgact tcaccgacat
    32821 cggcgatatc ctgggctgcc gcttccgcta catgaaggtg gccaagggca agctggagtt
    32881 cctgaaactc gtgtgacgaa cgggcagggc ggcgtcgcct gacgccgccc gccatcatgc
    32941 cagacagcgg gttgtacttg gaatggaagc gttacagctc atcgtgaatg gcctgatggc
    33001 ggggacgatc ctcgccattc cggccatcgg cctgaccacg ctgttcgcgg tgttgcgctt
    33061 caccaacttc gcgctcgcct cgcacatgac catcggcgcc tttgccgggt tcgtggccaa
    33121 cacgagcctc ggcctgccgg tgattccgtc gcttctggcg gccttcgtca tcgccggact
    33181 tgttggctgg ctttccgacg agttcatgct caagccgctg cgcgcgacgg ggttcatcac
    33241 cacggccatc ggctcgatcg cgctgacgat cgcgctcgaa aacatcgtgc gcttcatctt
    33301 cggcaacgcc atgcggggct acaatctgcc gctgcggcgc gattgggtga tccatgacat
    33361 tcgtgtcagt ccgcaacagg cggagaatct ggtcatcgcc gtcgtggtca tggcgctgct
    33421 gttcgcgttc ctcacgctga cccgaaccgg aaaggcgatg cgtgcggttg ccgacaaccc
    33481 catgctggcc gcgatcaagg gcatcaacgc ggacatggtg gcgcggctcg tttccttcgt
    33541 cggcatgggc ctggcgggcc tcggcggcat gctgatcggc ctcgacacga cgatcgatcc
    33601 tctcgttggg ttcaagacca tcctgtcggt gttcgcggcg gcggtcgtcg gtggcctcgg
    33661 cagcattccg ggggcggttg tcggcgcgct ggtcatcggc gtgggcgagg aactcagcct
    33721 gctggttctg acgccggcct accgcacggc ggtcggtttc atcgccattc ttctggttct
    33781 caccttccgg ccgcggggta tcctcggcca gcgggcctac tgagggaacg ccggcatgga
    33841 acactatctc gtcgccatgg gggttacggc cggcctctac gccgtgatgg cgcttgggat
    33901 gaacgtgatc tggggcatgg cgggtctgat caacctcggc atggtcgggt ttttcgcggt
    33961 cggcgcctac acgtctggac tgctgactct caagtttggc tggccaattc ccgccggcat
    34021 tgtcgccggc gcggcgatgg gcggtctggt cggcgtgctt gtggcgctga tcaccgttcg
    34081 gctgcgcggc gattatctgg ccatcgtgac cctcgggttc gccgagacca tgcgcatcgt
    34141 catgtcgaac gaattgtgga tcgccaacgg cacggatggc ctgtccggta ttccggggcc
    34201 gttccgtcag gcgctcggtc cgcacctgtt caatctgatg ttcctcggca ttgtcgtcgc
    34261 ggccaccacg atcctgttct tcctgttcga gcgtctgtcg catgcgccgt ttggccgggt
    34321 gctgcgcggc atccgcgaag acgaacaggt ggtgcaggtg gcgggcaaga acgtgatcat
    34381 gttcaaggtc aaggcctttg cggtgggcac cgcggccctc ggcatggcgg gcgcgctcta
    34441 cgggcatttc accagctaca tcgcgcccga cctgttcgcg cctctgctga cgctctacgt
    34501 caagctgtcg cttctggcgg gcgggctcgg caacaatgtc ggcgccattg tcggggcggt
    34561 actggtggtg ttcttcctgg aatcgacccg gttcgtcatt ccctacatcc ccgagatcag
    34621 tgcggtgcag ggtgccgcct tgcgggaaat cctcatcgcc ggagcgttgc tgctggtcct
    34681 cagggtcagg gcgcgcggcc tgatcccgga aaagatcgcc aatgtcccgc tgcctgatcg
    34741 gccgcacgcc gccctgtccg ccaaatcctg aacgagaacg ccatgccagc caagaccaga
    34801 gatgttaccc tcgacgatgt cgccgccatt gtggccatga ccgcggctga gcgcgaccgc
    34861 atgcaggtgt accgtgcggt tgagcggatc gccgccgaga ctgtcggctt cgttttcctg
    34921 acgacgctga agtacaacga ggcggaggcc tgcgtcgaac ggctgcattc ctcgaacgag
    34981 aaggcctatc ccgtcggcgg ccgcaagccc ctgtccaaga tcacggcgag tcacaaggcc
    35041 atggatgacg gcgaggtctt tctcgccgcg acgcgtgcgg cggtcaaaga ggcgttcttt
    35101 gaccacgaac tgatttttgc gctggggtcc acggccattc tcaacgcacc gatccgtttc
    35161 ggcggccgcc gcctcggcac cctcaatttc tgcggcgaag aaggaaagta cggtcccggc
    35221 gaggtgcgca atgccaagat cctggccggg ctgctgatcc cgtgcctgct ggcggaggcc
    35281 cctcggtgag gcgactgccg acgagattat tgcaagcatc ctgaaaaccg catcgagacg
    35341 aggagaggcg acgatggcgc tctatcactt caagaacttc cagatgctcg atcccgaaat
    35401 cggcgagttg gcaggagggc acgagatcat cgtcgaaggc gacaagatca aggaagtctc
    35461 cgccaaggcg atcaagtccg ccaaggccga agtcatcgat tgcggcaagc gcacgctgat
    35521 gcccggcctg atcgacagcc acgtgcacgt cgtgctgtcc gaggtcgtga tccgcaacat
    35581 ggaacaaatt ccactcacgt tgatgaccgc acgtgccgcc cgcctgatgg gagacatgct
    35641 gaatcgcggg ttcacctccg tgcgcgacac cggcggcgcc gactggggac tgaaggaagc
    35701 ctcggagaag ggctatttcg ccgcgccgcg cctgttcatt gccggcaagg ccatcggccc
    35761 gactggaggc cacagcgatg gccgacggcg taccgacatg ggtgcgcgct gtcattgctg
    35821 caatgcgttg gcgttctcga tgggcatcgc cgatggcgtc tcaggcgttc gcaaggccgt
    35881 gcgcgaggag atgcggcaag gcgccgatca gatcaagatc atgatgtccg gcggcgtcgc
    35941 ctcgccctat gatccgctcg acagcctgca attctcgctg gaggaggtca acgccgccgt
    36001 cgaggaagct catgctttcg gccgttacgt ctgcgcccat gcctacaccc cagaggctat
    36061 cacccgggcc gccaacgcgg gcgtgcgcac catcgagcac ggcaacctta tcgacgatgc
    36121 ttccgccaag ctcatggctg agaaaggcat gttcctcgtc gccaatctgg tcacgtattt
    36181 cgagatgaag aagcgcgccg ccgagtacgg catgaacgcc gacatgttgg ccaagaacga
    36241 catcgtgatc gaaggtgcct tgcgctcgct tgaaatctgc aaacggcatg gcgttcaggt
    36301 cgcgtacggc accgatctgc ttggcgctct ccagagcgag cagtcggagg aattctcgat
    36361 ccgtgcccaa gtggtgaagc cgatcgaaat cattcgctcc gccacgagca ttggcgcgcg
    36421 cctgctgcgg atggaaggca agcttgggtg cctgaaaccc ggtgcattcg cggacctgat
    36481 cgtcgtcgat ggcaatccgc tgaagaacct caagctgttg caggaacagg gcaagcacct
    36541 ctccgccatc atgaagggtg ggcatttcca caagaaccgg ttgaactgaa gcttccatgg
    36601 gaactcagct cctcctgacc aacttcgatc tgctcgaacc gagtttcggc gagatcaggg
    36661 gcggctatga gctcctcatc gaaggcgatg ttattcgcga gctttccgaa aggccgatcc
    36721 gcagcgctgg cgcgacgcgg atcgactgcc tcggcaagac attgctgccg ggtctgatcg
    36781 acagccatgt tcactccatt cattctgaag tgaacatccg cttcatggaa gccttgccgt
    36841 tgactctcct cacggcaagg gcagcgacac ggctgacggc catgctcaat cgcggcttca
    36901 ccaccgtgcg cgataccggc ggcgctgact ggggcatcaa ggaggcgctt tcgcaggggc
    36961 acattgtcgg cccccgtctt ttcatcgccg gccagtcgat cgggccgacg ggaggacact
    37021 cggattcgcg tcggcgcagt tcctccggcg gcgaatgctt ctgctgcaac ggcttgcagt
    37081 tcaagtccgc cgttgcggat ggcgttgacg aagtgcgcaa ggcagcgcgc gagcagatgc
    37141 gacagggcgc cgaccatgta aagatcatga tgtcgggggg tgtcgcatcg ccctacgacc
    37201 cgctcgacag ccttcagttt tcggacggtg aggtcatggc ggcggtggag gaatcgaaag
    37261 cgttcggccg ctacgtctgc gcccatgcct atagtgcgga agcaatcacg cgagcggcga
    37321 aactcggcgt gcgcaccatc gagcacggca atctgatcga cgatgcctcg gccaggttga
    37381 tggcggaaaa cggcatgtat ctcgtggcca atcttgtcgc atattacgcg atgagagagc
    37441 gcgccgccaa atggggcatg accgccgaaa gcctcgaaaa gaacgaagct gtcctgcaag
    37501 gcggcttgcg ctccctggag atctgcaagc gcaatggcgt cccggtagcc tttggttcgg
    37561 atcttctggg tgaactgcac tgggatcagt cgcgcgaatt cgaaatccgt caggaggttg
    37621 tcgcgccaat cgatatcatc cgctcggcaa ctcttgtcgg cgcggaaatc gtgcggatgc
    37681 caggccggat cggcaccctg ctgccgggcg cctttgccga cctgatcctg gttgatggaa
    37741 acccgctcgc aaacatcgct ctgctcgccg gtcagggaga aaggctgtcg ctgatcatga
    37801 aaggcggaac cgtgcacaag aacaggctca acgcatgagc aggctgacct cccgtcggct
    37861 cggcagcctc gctctcacgg gggcggcggg tctatcgctg gcctacatcc tgttgcccct
    37921 gttcttcgtc gtctggctgt ccttcttcca gcaggaaatt ccatcttttc cgccagaagg
    37981 atactctttg aagtggttcg cggccgtgcc cggcaacgac cggttcgttt ccgggtttct
    38041 cttgagcctg caggtggccg tcatcgccac ggccatcggc ctggcgctgg gcgtgccggc
    38101 ggccgtggcg ctggtgcgtg cccgcttttt cggccgcgag gcgctgaaca gcctcttgct
    38161 tctgcccctg gtcgtgccgg ggatcgtgct gggcatcgcg atctatgtct ttcacgtcga
    38221 ggcggaaatc ctcacggagt ggcacattct cggctcgctc ggaggattga tcgccgggca
    38281 tgtgctcatc gtcattccct ggacggtgcg gctcgtcacg gcgggtctcg tcggcatgga
    38341 tcgtgccatc gaggaggcgg caatgagcct tggtgcggac cgcttcacga cgttccggaa
    38401 aatcacgttg ccggccatcc tgcccgccgt ggtggccgcc gccctgttcg ggttcatcgc
    38461 ctcgttcggc aaccttgaga tgagcctgtt cctcgtcggg cccgggcgga cgacgctgcc
    38521 gatcgccatc ctgcagtatc tggaatggaa gatcgatccg acgatcgcag ccgtatccgt
    38581 gctgcaaatc ctgttcatcg gcggcgcgat gctgatcacc gatcgcttcg tcaagatcag
    38641 ccgggtggtg tgaatggcgc ggcttgaact gtcctccctc gtcaagcgct acggcgatct
    38701 gcgcgccgtc agcgatgtct cgctgaccgt cgaggacggg gaattcgtgg tgctgctcgg
    38761 gccgtctggt tgcggcaaga cgacgacatt gcgcatcgtt gccggcttcg tcgagccgac
    38821 ggctggatcc gtgcgtctcg ggcagcggga catcacgcat cttccgccct ggaagcgaaa
    38881 cgcgggcctc gtgttccaga gctatgcgct gtttccccat ctgacggtga acgagaacgt
    38941 cgcgttcggg ctggagatgc gcaagatgcc ccctgccgag atcgctccgc gcgtagcgga
    39001 ggcgcttagg ctggtacggc tcgcccatct cggcgaacgc ctgccacgcc agctttccgg
    39061 cgggcagcag cagcgcgtgg cgctggcgcg ggctctggtg ttccgccctg acatcctgtt
    39121 gctcgacgaa cccctctcca atctggatgc gaagcttcgg caggacgtgc gcgtggaaat
    39181 ccgcgagctg cagcgcaaga taggcctgac gaccgtgatg gtgacgcacg accaggaaga
    39241 ggcgctgacc atggccgacc ggctggtggt gatgagcgat gggcgcatcc gccaggtcgg
    39301 ctcgcagcag gatctttatg aacggcctgc ggatcggttc gttgcggatt tcgttggccg
    39361 ttcgaccttt ctcgaaggcc atattgagac gccagggcgt ttcatcacgc agggtgggtt
    39421 gaccatcgcc tgctcggggc tggaaactgg cccggccgcc atcgccctgc gaccagagcg
    39481 catcgccctg cttgcaggcg aagagaaagc cgacaacgcc ttcgaggggc gtgtggagtt
    39541 tgtctcctat ctcggagcgc aggtcgatgt gcatctgcgc ctcaatgcca gcgagcgcat
    39601 cgtggtccag gtccccaatc ggccggaccg gacattgcca gcgatcggcg aagccgcgag
    39661 aatcggctgg agccgcgaaa ccggaagggt gttttccggg gctgcgacga cggactgaac
    39721 agggaataag tcaccaacag ggaggacatg accatgacca gcacattcca tctcaaccgc
    39781 cgccagctgc tcggcgcagc agcaggcgtt ggcgtaacag gcctgcccgg cgcggcgctg
    39841 gcgcagggca agggcaagat cgtggtcggc acctggggcg gcgactatgc ccgcctgctc
    39901 aacaagaaca tcgagcaacc gatcctcatc aagaacggct gggaggtcgt gcaggatcag
    39961 gccggtgatc cgcagcggcg ctcgaagatg ctggcggaac agcgcctgcc gcgcggcacg
    40021 tccgacgttc aggggctgtc ttcgatcaac atgttccaga tgcacgatgc cggaaccgtg
    40081 ctgccgatcg actattccaa gctcaagaac gccggcaacc tgctgccttc gatgaaatac
    40141 ccctggggcg tcggacacat ctattcgggc aaggtcgcgg tatataatcc gaagatgatc
    40201 aacccggcgc ccaagagtta caaggacgtt ttcgacccca aacacggcaa caagctggcg
    40261 ctgatcgaca tccagtacca gtatacgatc gtctgcgccg ccctggcggc cgggggcagc
    40321 acgacgaacc tggagcctgg caagaagctg ctgctggaat gcaagaaggc cggcatgcgc
    40381 atgtatccga ccaacgaggc cttcgccgcg ggcctcaagt cggaggagtt cggcatcggc
    40441 gtgatgtgga aggcgcgcgt ggtgcagtgg cagaacgcgg gcatcagcgt cgaggcggtg
    40501 gcgccgtcgg agggcacgct cgcctatgtc tcaggctttg tcattccgaa gaactcgccc
    40561 aacaaggacg gcgcctacgc ctatgtcgat gccatgctcg aaaagagcgc ccaggaggcc
    40621 ttcgcggtcg acatgggcta caacccgacg gtgaccaacg ccgtcgtggc gccggacctc
    40681 aacaagcgca tcggcttcac gccggacgag atcaagaagc tggtcgatct cgattacgac
    40741 cacatgaaca agaacgacac cgcgcttaag gaatggtggg acaaggagct caagggctga
    40801 cgttgaagca gggccatcgc cgccgccggc tgcgatggcc cgcaaccgcc gaaaaggcgc
    40861 aaccggaacc ctgatacgcc atggcagccg ccgccgcaca cgcgcccccc gaggacggac
    40921 gaacgtcgat cctgtcggca tcgatgctga ttgggccggc gacggtgttc gtcgtgatcg
    40981 gactgctggc gccactggcg atcctgttcc gctacagcct caaccgcttc gagccgcggc
    41041 gtatgatggt cgaggccttc acggcggaga actacatccg gttcttttcc gatccgttct
    41101 acaccggcat cttctggacc actttgcgcg tcgcggcgct gtgcacgatc atctgtctgg
    41161 tgatgggcct gccactggcc tacgtgctgg cgcgcacgca gacccgctac aagaacattc
    41221 tgatcatgct cgtggtgctg ccgctgttcg tcggcaatgc cgtgcgcgcg gccggctgga
    41281 tgacgctgtt cggctcgaag ggcttcctga acgtctcgct tatgcaactc ggcgtgatag
    41341 accagccgat gcagatcatg ttcaccgagg tcgcggtgat cttcggcatc atcgccgtca
    41401 acctgccgta tatggtgctg acgttgcaaa gcgtgatcga gggcgtcaac cgcaatgtcg
    41461 aggaggcggc attcagcctg ggggcagggc ccgtgacgat gttccgccgc gtgctgctgc
    41521 ccttggcgct gcccggcatc ctggcaggaa ccatcctgac cttcatcctg ggtatgaatg
    41581 cctatgccac gccggtgctg cttggcggtc cgaaattcaa gatgatgggt ccgctggtct
    41641 tcggtcagtt ccagctcaac aactggccct tcggggccgc cattgccttc gttttgatga
    41701 cggcgacgct ggtgctgacg gcgtctgcaa acctgatggt gcagcgccgc taccggcgct
    41761 gaggagtgaa catggcgctc gaacgcggaa tggtggttgc ggcgcaaccg gaggcagtgg
    41821 aagctggcgc agaggtgctg gcggccggcg gcaatgcggt cgatgcggcg gttgcgaccg
    41881 ccttgaccca gaccgtggtc gatccgcaaa tgtgcggcct ggccgggttc ggctgcatgc
    41941 atgtctacga tcccgcccgc gcccggcacg agaccatcga tttctatgcc cgcgtgccac
    42001 gctcggccaa gccggacatg tgggaagcca agctcgtgtc ggagtccgac gacggcttcg
    42061 gcttcttcct gtccgatcgt tcgaacgaga tcggctatgg ttcgatcgcc acgccggtca
    42121 cgctgcgtgg cctcgatctg gcactggcgc gccacggcac gctgccgctc gcaacgctga
    42181 tccagcccgc catccgccat gcgcgccagg gcgtcatggt gcgaccgcat atggccgcct
    42241 attgggggca ggtgccgacc gaaagtcgcg cgccccatca ggatttcctg accgcgttgc
    42301 cggcgacgcg gcgcatctac gcgcatgagg acggcagcat ccacaaggtt ggcgagaccc
    42361 tgaagaatcc ggacatggct cgtacgctcg aacggatcgc cgagcatggc gcggaggatt
    42421 tcttcaccgg cggcatcgcc gccgagattg ccgccgacat gcgggcgaac ggcgcgctct
    42481 tggacgccga cgacgtggcc aattcccgcc cggtgatcgg cgcgccgatc tggggaacgt
    42541 atcgcgggct gcgcatcgcc agcaatccac cgcccggagg cggcgtgaag ctgattgaga
    42601 tgctgaacat cctggagcat ttcgacctgg cggccatggg gcacaattcg ccggcctata
    42661 tcgccaccat cgccgaggcg atgaagatcg gcgcggttga tcgcgacgcc acccacggcg
    42721 atccggaatt cctcgacgtg ccggtcgcgc ggctgacatc gaaggcttat gcagcggaga
    42781 tggccgcccg catcaagagg ggcgaaaaga cccatgtgcc gcgcctgaag gcccgcgaag
    42841 cggccgatac gacccacgtc tgcgttgtcg atcgcacggg tctttgcgtg tcgctcaccc
    42901 acacgctcgg cacgccttcg ggcgttgtca ccgaagggct tgggttcatg tacaacggcg
    42961 cgatgggtgc cttcgatccg cgcccgggcc gggcagggtc gcttgcgccg ggcaagcggc
    43021 gcatgtcctc gatggcgccc tccatcgtgt tcagggatgg caagccgttt ttcatcgtcg
    43081 gcgctccggg cggcacctac atcacgcctg gcatcctgca ggcgatcctg aacgtcgtgg
    43141 attttggcat gagcgcgctg gaggcgatct ccgccccccg cgtctgcgcg acatcggaca
    43201 cgatctggat caccaaccgc atcctgcgtt ccacggaagc ggcgctgcgg accatgggct
    43261 atccggtgaa gcgctcgcca ttcaactact acttcgctgg cctccacggc attcgcgtcg
    43321 tcgacggcgt cccagatggc gccgccgatc cgggccgcga cggcatggcg ctggcggtct
    43381 gaaaccatcg ggtgaaacgg agacgaagtc atgacgattg gcgtgggcgg ctcgacgccc
    43441 gagaaggaac tcgcggcgtt gaagaacatg cgcggcgacg ttccgcccat tggcgtggac
    43501 gagcgcctca ggcgtatcga caaggcgcag cggctgatgc gggacaaggg catcgacgcg
    43561 ctctggctcg atgtctcttc gagcctgacc tatttcaccg gcctgaagat gcgccgcacc
    43621 gaacgcgccc acggcgcgat cttgcctgca aacggagaga tcatctacct gtcgccggcc
    43681 ttcgaggtcg agaagctgat gaccatggtg acctttgggg acaaggtcgc gcgctgggaa
    43741 gaagatgagg acccgacggc gctggtgatc gacagcattc gcgcgctggg tgtcgagcgc
    43801 ggcgtcatcg cggtcgacga ggcgaccccg ttcttcacct tcgacggttt gcggcgggcc
    43861 ggaaacgcct tttccttcgt gaacggatcg gacgtcacgg cggcttgccg gatgatcaag
    43921 tcggatcacg aaattgccct gatgcagacc gcgaagaaca tcaccctgga ggtgcacaag
    43981 gcggcggcac gcatccttag cgaaggcatg acaacgaccg aagtgcaggc cttcatcgcg
    44041 gccgcgcatc gcaaggccgg ttcggaaggc gatgtcggat tcaacggcgt gctcttcggc
    44101 gaagcgacgg cctatcccca tggcgttccc tatccgcagg tcctcaagcc cggcgacatg
    44161 gtgctgatcg acaccggtgc gccgatcgac ggctatctgt cggacatcac gcgctcgtat
    44221 gtgtttggcg aaccgtcgca gcgtcagcgc gaggtctgga acctcgaaaa ggcggcgcag
    44281 gctgccggtt tcgctgcggc gaagcccgga aaccgctgcg aggacatcga catcgctgcg
    44341 cgcggcgtca tcgccgccgg cgggttcggc cccggttacg cgacgccggg cctgccgcac
    44401 cgcaccgggc acggcatcgg cctcgacgtg cacgaatggc cctatctggt gaagggcgac
    44461 cgcaccgttc tccagcccgg catgacgttc tcgaacgagc cgacgatctg catctacggc
    44521 gagttcggca tccgcctgga agaccacatg gtcatcaccg agaccggtgc gcgctggttc
    44581 acgcggccgg cccactcggt ggacgatcca ttcggactgg atgtggggta ggggccaggc
    44641 gagcggtttg agcaggcctg cgccgggggc tccggttcta ggcaccctgt caagtcgagg
    44701 ccagcctcgt tcttgccacc gccgtcagcc gacaggcggc gtcccgtggg tgtgaaagct
    44761 ctcgattgtc ttcagccccc aggcctgccc tttcttccgc tccgtctccg tccaggtcac
    44821 gatctgccag tccggatcga ggatcagccg cgcgccggca tttgccactt ccacgcgatt
    44881 cccggcgggc tcccagacgt agatgaagaa tgtctgctgg accgcgtgct tgtgcgggcc
    44941 ggtttcgatg tagacgccgt tctcgaggaa gacgtcggcg gcctgaagca cctgctcgcg
    45001 ctgatccacg gcgtaggtca cgtggtggaa tcgcccccgc gagctcgtca tgtcgcgcgt
    45061 gtaggcaatg tcgtatgtct tgttgttgac ggtgaaccag cacccgccaa gcttgcctga
    45121 atcgagcagg atctgctcgg tcacgcggga gccgagcgcc ttcggaatga agtctcggat
    45181 ggcatcgacg tcctgcgcga gcaggttgag atggtccagc cggcgcacgt tgcacccccg
    45241 accatgctgg cgctgcgcct ggttcttcag gcttgggcgc tcagcgggcg gtgcctcgta
    45301 tttccgggtg tcgaaataga cctcgaagat gtggtcgtcc ggatcgcgga aacggtaggc
    45361 ccgcccgtgg ccgagatcgc cttcggtcca gccaattccg cagcccatgg cttcgatcgc
    45421 cgccacgcgc cggagcaggg cggcttccga cgatgcgcga tagccgatat gcgcgactcc
    45481 ggtggtccgc gatgccgtca gtttcagcgt gtggaactca tagtcgtccc aaccgcgcag
    45541 atacacagat tcaccctcgc gcccgctttc ggtcagcccg agaacattga cgaagaagtc
    45601 gaggctttcc tgcggcttgt tcgtcagcat ctcgacgtgg ccgaggtgcg cgatgtcatg
    45661 gcacggttct gtcatggcac tcactgctgc tggatgccgg cgcggcggat cacctcgccc
    45721 caacgggcga tgtcggtctt caggcgttcg ccgatggcgg cgggcgtgcc acccccaggt
    45781 tcgattccaa ggtcgagcaa cctctggcgg acaccggcgt cgcccagcga ggagacgatg
    45841 gcctcgttca gcacgcgcac gacctccgcc ggcgtccctg ccggcgcgaa gactgcgttc
    45901 caggatgtga catcaaagcc gggaacgccg ctttccgcga cggtcggaac gtcggggaga
    45961 ccggcgaaac gccgtggacc ggatgtcgcc agggcgcgca gcttgccctc ctgcatgagg
    46021 gctttggcgg ccacatagcc gtcgatcacc aggtcgacat ccttgcgaag cagcgccgtg
    46081 atggcgtccg gcgtggtcct gaacggcacg atctccatgc tcacgttcgc catcgatctg
    46141 aagaggttcg ccgaaaggtt ctggctggac ccgacgttga tcgtgccgat gttgagcgcg
    46201 ccgggccgcg ccttggctgc ggccagaaga tccccaaggg tcttgtgggg gtggtcaccc
    46261 gacgtcagaa tcaggaagtc gaagaaaccg agcgatgaca caggcgcaaa ctgggtcagc
    46321 ggatcatagc caagctcttt cacgagtggc acgctgatcg cggtgccgtt ggtgaacagc
    46381 accagggtat agccatctgc cggcccagtc agcgccgtct gcgccgccgc gacgccgccg
    46441 gcgccgggct ggttcacgat gacgatccgc tggcccagca cctggctgag gcgatctccg
    46501 acgacgcgcg aggtgatgtc cgccagtcca ccggggccga agggcacgat gaggcggatg
    46561 ggcttgtcgg gataacgggc ctgcgcgcga agaggagcgg cgccggtagc tgcggcagcg
    46621 gctgcggcga tcaacatgcg acgggtcagc atttctggtc tccttggaat ccgtaaagcc
    46681 tcgccgggtt ttcgacgagc agccggtgca gggctttcgc gtcgccgtac tccggcaaca
    46741 tgtcgacgag atcggcttcg tcgacctcgt gcttgaggtt gggatgcgga aaatcggtgc
    46801 cccaaagaac gcggtccggc gcgacgttca tcaaggcgcg ggcgaagggg atggcggcgt
    46861 ggaacggcgg gtcggcaatg cgttccgcgc ccgagacctt cacccagaca tgcgcgtgcc
    46921 ggagcaggcc cgttaggtgc cggtgcggtt cctgggcaat cccgtccttt gccggcgtgc
    46981 ggcccatgtg gtcgatcacc gcgatgaccg gcagcgccgc gatcatgggc gacagggctt
    47041 cgatgtccgg cgcgtcgaga tgcaggacca catgccagcc gaggggagcg atgcgccgga
    47101 tcacgcggtg gaaaacatcc atgtccggcg cgccgcccag gtgtttgacg aaattgaagc
    47161 ggacaccgcg aacgccgccc tcatgcagca cggcgtaggc gtcgtcgccg aatgtgtcgt
    47221 cgacgatcgc cacgccgcga taggtttccg gcgcgcttgc gatgcagtcc agcatcgcgc
    47281 gattgtccgt gccgtggcag gaggcctgca cgatgacggc acgggaaagc ccgagacggg
    47341 catgcaggcc cgctagcgct tcctttggcg cgtcctgcgg cgtgtatcgc cggtcaggag
    47401 cgtaggggaa ccgaccggcc gggccgaaga cgtggcaatg cgcgtcgcag gcgttggccg
    47461 gcggcgtgta gagcggccgt ctttccaggg ctttcccgca ggacatcagc cccagacttc
    47521 cttcgcgacc tcgacgacgc gctgcagttt cttccattgc gactcctcgg cgagaagatt
    47581 gccctcttcc gtcgaagcga agccgcactg tggcgacagg cagagctgcg agagcggcgc
    47641 gaacttcgcg gccttctcga tccggcgctt caggctgtcc tttcgctcca tgtcgccgac
    47701 tttggtggtc acaaggccga gcaccacatg cttgccgcgc ttcggcagga agcgcagcgg
    47761 ctcaaagccg ccggagcgct cgtcgtccca ttccatgaag tagccgtcca cgtcgatctc
    47821 gttgaacagc atgtcggcga ccggctcgta gccgcccgag gcgatgaagg tcgacttgaa
    47881 gttgccacga caggtatggg tcgtgatcac catgtcgtcc gggcggtctt tcgtcgccat
    47941 gttgatgacg tcgcggtagg cggtcatcag ccgcgccgga tcatccccgc gcttggacag
    48001 catgtcgcgc tgctcgggat cgcagaagta ggacagcgag gtgtcgtcga gttgcaggta
    48061 gcggcagccg gccttgtaaa aggtcttgac cgccttggcg taggccttgc cgagatcatg
    48121 gaagtattca tccatatccc ggtagaccgt ggcgttgatc gaggcgctgc caccccgata
    48181 gtgcagcatc gacggcgagg ggatggtcat cttcggcacc ttgccggtga ccttcgccag
    48241 atacttgaaa tgcgcgatgt gcggatggtc cgtcgggaag tcgatcttgt tcacgacgcg
    48301 gatcgagtga cccttggtgg tgacgccctt gaacgcgatg cccgcgctct cgaccccttc
    48361 gacgcctgtc aggtgctcca ggaagtcgaa gtgccagaag gcgcgccgaa actcgccgtc
    48421 agtgaccgcc tgaaggccga tctcttcctg ctttttaacc agcgccttga tctcggcatc
    48481 ctcgaccttt ttcaggtccc cttgcgaaat ctgcccggcc tcgcgcttgc ttctggcgtc
    48541 cttcagcgcc tgcgtgcgga gcaggctgcc caccatgtcg gcccggaagg gtggcttgcg
    48601 cttcatgtca tgtctcctcg tcttgccaga tgatctgttt ttccatcgtc tgcggccgcg
    48661 aggccgagat agcgttcgag cggcgcggca tcggtcagca gcgccgcgct ttgcgacgcg
    48721 tgcacgatgc gcccccgttc caggatcacc gccttatcgg tcagtccgag gatcgtgcgc
    48781 gcatgctgtt ccacgatgat cgacgccagg ccttcctcgc ggcgcagctt cgtgagcgcg
    48841 gcgagcagtt cctggatgat gatcggcgcc agtccctctg tcggctcgtc gagcagcagc
    48901 agcctggggt tgagcaccag tgcgcggccg atggcgagca tctgctgctc gccgccagaa
    48961 agctggctgc cgagattggt ctttctttcc gccaggcgcg ggaaaagctg gaacacgcgc
    49021 tgcggtgtcc aaggacccgg tatggccacg gcggtgaggt tctcggtcac cgagagcgtg
    49081 cggaagacgt tgcgttcctg gggcacccag cctaccccgg ccagcgcccg cgcttccggc
    49141 ggcttcgccg aaatatcctg tccgccgaga cggatgcggc cgccgtggcg ccgcgtcacg
    49201 ccgatgatgc tgttgacgag tgtcgtcttt ccgacgccgt ttcggccgag aagggcaagg
    49261 gactggcctt cgcccagcgt cagggcgatg tcgtgcagca cgatcgcggc gccgtagccg
    49321 gcggaaaggg cgtcgatttc cagtagctca gccatgcatg gcctcgccga ggtaggcggc
    49381 cttgacgtcg ggatcggcgg cgacgtcctg cggcaggcct tcggtcaaca ccctgccgtt
    49441 gacgagcacg gtgatccgcg tggcgaaacg gaacaccaca tccatgtcgt gctcgatgag
    49501 cagcaccgaa acgtcctcag gcagttccgc cacgctgtcc agcaacagtt tgcgctcggc
    49561 ttccggtacg ccggcagccg gttcgtccag caacaggacc ttcggcctga cggcaaaggc
    49621 cgatgcgatt tcaatctgcc gctgccgccc gtaggggagc gtgtcgacgc gctcgtccat
    49681 cacgtcggca aggcggaagc gctccaggat ggctgcggtc tcgctgatgg cggcgcggtc
    49741 agcgtcgaga cgtccgaaca ggcgcccgcc gtgtccgagc cgctcggtca cgaccatcat
    49801 gatcatttcc agcacggtca gcccggcgca gagctggttg atctggaagg tgcgggcgag
    49861 tccgagtttc acccgggctt cccgcgacag gctggtgatg tccagaccgt tcatctcgat
    49921 acggccctcg gacggcgtga tgacgccggt cagcaggttg acaagggtgg tcttgcccgc
    49981 gccgttcggc ccgatcagcg catggcgcgc gccctgttca agcgtgaacg agacgtcatc
    50041 ggacgcgacg atgccgccga agcgcttcgt aaggccctgg gtgcgcaaga cgacgctcat
    50101 ggcctgcgtc cccccggcgc cagcgaccgg atgcgtcggg caaccgcgcc gactccgccg
    50161 tagatgacct cgcggccgcc gaggacgaac acgacgagca gcaggcctat ccagaactgc
    50221 cagtattgcg gcgtcagcgc cgcgatccag tcctgcagga acttgaatgc gatagcgccg
    50281 ataatgccgc cgtagaggta gcccgtgccg ccgatgatga gcaccagcat caggtccgca
    50341 gagcgatgga aggcgagggc atcgagcgag acgaattgcg tggtctgcgc catcaaggcg
    50401 ccggccgctc cggcataggc cgcggcaagg ctgtagatcg ctgcaagccg ccagccgggc
    50461 ggcacgcccg atgccgcggc ccgaagctgg ttatcgcgga tcgcgcgcag cgacaggccg
    50521 aatggcgaat gtacgatccg gcgcgcgatg acgaacagga tgaagaggac ggcgagggag
    50581 tagagatagg cagtcttgcc cagcagatcg aactcgaagc gcccgagaac gggctgcatc
    50641 gtgatgccct ggaggccatc ggcgccgccg gtccacttgc cgagcgaatt ggcgagctcg
    50701 tgcagcagtg aggcgatgcc gagcgtcacc atcagccgcg tcaggtcatt gccgcgcagg
    50761 atcagcggcg acgtcaccag gccgagcgcg gccgaaatga cgatggcgaa gcccagcccc
    50821 gcgatgggat cggccgtcac atgcagcgcc agcagccccg cgccgtaggc gccaaatccg
    50881 aggaacgcgg catggccaag cgacacgatg ccggcatagc cgaggatcag gtccagcgag
    50941 atggcgaaaa gcgcgaggat ggcgatttcg ttcaggatca gcagttggcg cggaaagatg
    51001 aagaaggccg cgagcgcaaa cagccagaac agcgactcga acggcgacca gcggcgcgac
    51061 agcgtcagcg aggtccggat gcgggattca agcgaagggg cgttcatgtc cgcgacctcg
    51121 cgaacagccc ttccggcttg acgatcagga tgaccaccat gatcgcgtag atgatgaagg
    51181 cgccggccca ggggatgtag tatttgccgg caacatcggc gacgcccagc agcattgcag
    51241 cgaggaatgg gccggtcagc gtggacgtgc cccccacggt caccacgatg aggaaataga
    51301 tcatgaactt gagcgggaac gtcggatcaa ggcccatgag ttcgattccc agcgcgccgc
    51361 caagcccggc aagacccgag ccgatggcga aggtcaccgc gaagagctgg ctgaccggga
    51421 tgcccaggcc acgcgccacg cgagcatcat cgactgcggc gcgcaggcga ctgccgaaac
    51481 gcgtccgcgt gaaggcgtac tgcagcgcga gcacgagcag gccgcagagg atgatgacga
    51541 aaagccggta gcgcccgaca gcaatgcccg cgacttcaaa acggccctgc agccacggcg
    51601 gcaaccggat gatctgttgc atcgacccca tgacgtagtc cgtggccgcc acggccatga
    51661 agaccaggcc gatggtgaaa agcacctgat cgaggtggct tttcgcgtac atcggccggt
    51721 acagcagccg ttccagtatg acgcccgcca gggcggaaat gaggaacgcc gccggcagcg
    51781 tccacaggaa tggcacgccc aggcgctgca tcagcacgac cgtcacgtag ccgcccgcca
    51841 tggcgaaggc gccgtgagcc aagttgacga agttcatgag gcccatggtc acggacagac
    51901 cacaggccag cacgaacagc accatgccat acgcgatgcc gtcgaacaga agcgtcagca
    51961 tggagccggc gactccgcca gtgcgcggat tggactgcgg cggctcactt cttcgcggct
    52021 ttcgcggggt ccttcacgtt ggggatcgtg tggaactcga cgttgtggag ttcgccgttc
    52081 acgcgctcga ccttgcggat gtagacgttc tgaatgatgt cgcgggtctc cggatcgatc
    52141 gacattgggc cgcgcgggct ctcccagctc aggcccttca tcgcctcgac cagcgcctcg
    52201 ccgccctgtc cgttggtctt cttcaggccc tcgtacagcg catgcatgcc gtcgtagccc
    52261 ccaacggaca tgaaattcgg ccgcatgcct ggattggcgc gctggaacgc ctcgacatag
    52321 gccttgttcg tggcgctcgg gtgggccgcc gagtaatggt gcgcggtcgt gacgccgagc
    52381 gcggcatcgc ccatcgcggg caggatgtcg tcgtcggtca cgtcgccggt gccgatcagg
    52441 cggatgccgc tcttgtcgag accgcgctcg gcgaactgtt tcatgaaggt cgagccttgc
    52501 cccgatggca cgaagatcag gatcgcttcc ggccgcgcgt cggcggcgcg ctgcaggaag
    52561 ggcgagaagt cggggttctg caacggaatg cgcatcgcct cggtctggcc gccggcctcc
    52621 ttgaacgttg cggtgaagga agcctcgacg tcgtgccccg gcgcgaaatc cgacaccatc
    52681 gtcacgatcc gcttgatgcc ctgctgggcg atccaggtgc cgaacgggcc ggaaatctgc
    52741 ggaacgacct ggctggtgcg cacgatgaac ggcgagcgct ccgtgatgat cgacgtggcg
    52801 gcggccatca cgaccatcgg cgtcttgccc tgtgtcgcga ccggcgccgt ggcgagcgcg
    52861 atcggcgtca ggccgaaacc gccaatcgcc gtcaccttgt cattgacgat cagttcctgc
    52921 gacaggcgac ggccgacatc cggaacgccg gtatcgtcct tgatgatcaa ctcgaccttg
    52981 cggccggcga cactggcgcc cttctgcgcc atgtagaggt tggcggcggc ggcgatctgc
    53041 ttgccggttg aggtgaaggg gccagtcagg ggcaggacga ggccaatctt gatcggctcg
    53101 ccctgcgccc tggcgatcga aggcatcatg cccaccgccg cggttgcgcc gattcccttg
    53161 aggatcgtcc tgcgttccat tgcttcctcc tcttgccggc cttttgccgt ccggctgttg
    53221 tggtacttct aggtgttgtt ccatccgcgg tagacaaggc cggccttcgc cagcccttcg
    53281 cgcatgccat acatgtcaag accaagctcg ccggcggcca gccgacgccg cttgtccact
    53341 tcgttcgcct cgcgcgcaag accggccgcc gcgaccttgg cggcatcgag gcgcggcacg
    53401 acgaccacgc cgtcatcatc cgcgatgacg acatcgccag cctggacaag ggcgcccgcg
    53461 cagacgatcg gcacgttgac cgagccgagc gtcgccttca ccgttccctt ggccgagatc
    53521 gcccgcgacc agacaggaaa acccatctcc gtcagcggct ttacgtcgcg cacgccggca
    53581 tcgatgatca gcccgcgcgc cccgcgcgct ttcgccgagg tcgcgagcag gtcgccgaac
    53641 atgccgtccg tgttgtctgc cgtgcagccc acaacgagca cgtcgccggg ctgcacttgc
    53701 tcgatggcga catggatcat ccagttgtcg ccaggttggg ccaggatggt cacggccgct
    53761 cccgcgatgc aggctccggg gtagatcggc cgcatgtagg gcttgagcag gccgatgcgg
    53821 cccatcgcct cgtgcgttgt cgagacgccg agccgcgcga gggtctcgac agcagccgcg
    53881 tccggacggg gcacgccagt gatgacggtc ggcgtcatgc cagtgtctcc agcgcttccg
    53941 ggaacagccg ctggtaggcc tcgccgtagg tcatcgcctt gcccgagttg cgcccgccct
    54001 gcatgccgcg attgagggcg acgcgcgtgt agtagtccca gaggtgcttc tgcgccgcga
    54061 gaacctcgaa ggccttgcgc ttcgtctccc agacctcatc gatgttgagg atgacgttcg
    54121 gcttgaagtt gcactgctcg ggctggtgcg gttcgaacag gaacaccggc ggcgcggcat
    54181 aggcccggtt cgggtccgga ttgtggccgg ccgcctgcgc gatgatgcgg gcctcctggg
    54241 cgaagcgtgt cgcttccgga tgatcgacat tataggggtc ctcaaggctg tgcgtgagga
    54301 cgaaactcgg attcatatcg tggaagatgt cgatggcgcg atcgagcatc gcctggttcg
    54361 tgcgcagggg atagtcgccg gcatccatga attcgatctc ggcgccgagc acggccgcgg
    54421 cccgttccgc ttcgtccttg cggccggcct tgacctcggc aagggtcgcg ccggccttct
    54481 tccaggcgaa ctggctttcg ccgcgttcgc cataggacag gcagagaatc ttgatccgca
    54541 tgccgcgccg ggcatgcagg gcgatcgcgc cgccggcgcg ccagacgaaa tcgccgggat
    54601 gggcagtgat cacaaggcct gtcttcgtca tggcactcca gtcctattcg gccgccgcga
    54661 gccggcgctc cggggcataa gcggttccat cgaaaagcat gcgcgcggtt ctcagcaagc
    54721 cggcccgttc gatcaccggc cgttccggtg tgccgccgac cgtgaggttc acggtgaatt
    54781 cgcccgtcgg atgctcgacg acgacggccc gttcccgacc ttccggcatg cgcgtcaccg
    54841 tgctggcggg agagccgggc agcgccgccg ccgtggcgac ggtgacggct gcgaagacgc
    54901 cgatcgaggc gtggcactcg tgggggatga aactgcgcgt ggccatgctg ccgccggctc
    54961 gcggctcggc gacgaggcag atcttgggaa cggccttctt cgaaacatcg ccaagagtca
    55021 ttatcggtcc ggccttcagt cggatggatt cgatccgcgc cttgagttcg tcgtccttgt
    55081 ccagttgctc gcgggactcg tgaccggtcc ggcccatgtc cgctgccttc atcacgatca
    55141 ccggcatgcc attgtcgatc agcgtgcacg gcaccccgtc aatcacgtcg acggcatgcc
    55201 cggttggcag cagtgacccg cacacggagc cttccgtgtc gagaaaggtc acggtgatgg
    55261 gcgccgacgt tcccggcgcg ccgtcgatcc tggcgctgcc atcgtagttc acgcggcccg
    55321 ccggagtctc gatcagcagg tcggccacgg ttccggtgtt caccgtccgc acacgcacgt
    55381 tcgtcttcga ctccagcgcg cgcaccagcc cgcgctcgat cgcgaaaggc gcgacgccgg
    55441 ccaggatgtt gccgcagttc ggcgtggtat ccaccatcgg cctgtcgacg ccgacctgtg
    55501 cgaacaggaa gtcgacatcg atcccgggtt cggtggagcg cgagacgatc gccactttcg
    55561 acgtcagcgg atgtgcgcct cccagcccgt cgacctggcg cgggtccggc gaccccatga
    55621 gcgccagcag cagcgcgttg cgctcggcct cgtcggccgg caggtcgtcg gcgaggaaat
    55681 aggcgccctt ggaggtgccg ccgcgcatca gcatgcagcg tacgccgcgc aacgccatct
    55741 cagcccccat cggtgatgcc ggcgcggcgg atgacctccc gccaggtcgc gatttcggac
    55801 tggatcagcg cgccgaaacg gtccggcgtc gcgggcgccg cgatggcgcc gtcgttgcgc
    55861 agggtcgtct ccacttcggg cgagccaagc aatgcgttga tctccgcgtt catgcgccgg
    55921 atgatggctt ccgggacgcg cgcgggtgcg acgagtccgt accactgcag ggcttcgaag
    55981 ttcggcaggc cgctctcggc gatggtcggc aggtccggcg cg
//
LOCUS       DAOMXN010000003        55974 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_424_length_55974_cov_11.533289, whole genome shotgun sequence.
ACCESSION   DAOMXN010000003 DAOMXN010000000
VERSION     DAOMXN010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 55974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 55974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55974
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_424_length_55974_cov_11.533289"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="PK812_00645"
     CDS             <1..249
                     /locus_tag="PK812_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68088.1"
                     /translation="ASKLPANERKAIEAMEAASARGTALSPSLRARISAQVTQGTQSA
                     GPKQQLAMRPALAGRTSKGSSGQDLRYVGPDVDFDRAR"
     gene            499..1521
                     /locus_tag="PK812_00650"
     CDS             499..1521
                     /locus_tag="PK812_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRJ68089.1"
                     /translation="MHSKSLRPAALAAALFCTPAVAQEVNVYTYREPALLKPLLDAFT
                     AETGIKVATIFAKEGLEERIAAEGTRSPADVFMTADFASLERARTMGLTRQVNSEALS
                     RAIPPGYRDAQGHWYGLTYRARVAYVSKARVSETQLTYEDLADPKWKGRFCLRSGKHI
                     YNNLLFAAAIAHMGEAKAEDWIRGLKANLVRKPSGGDREVAKDIASGACDIGFANTYY
                     MGHMLNGAPDQKPWAEAVRVVLPKFRNGGTHVNLSGIAIMKNAPNAANAQKLAEWLVG
                     EKAQALYSKVNYEFPVRAGVPIDPAIAGFGVLTPDTLSFEAILKERTKAADVVDRVGF
                     DGGPGS"
     gene            1518..3158
                     /locus_tag="PK812_00655"
     CDS             1518..3158
                     /locus_tag="PK812_00655"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRJ68090.1"
                     /translation="MTGASAAIPSALGRRSSSGGVLLRAASLALYVLLLLPLAGLTVL
                     AFAQPVEGHVLSALRLASGETLTLLLGVGVLSASVGVGAAWLIAMYRFPLRDILAVLL
                     VAPLALPTYLAAYVAVELTDYFGPLQSALRLIGGYTLRREYWFPDVRTLPGAILVLSL
                     VLYPYAYLAARIMFERQGSTLIQAARLQGCTGLRLFLRIGLPLARPAVIGGGVFALLE
                     TLNDIGAVEHLGVQSLSLAIKSVWLNRGNLPGAAQISLLCLVMVVAILMAEHAFRRHA
                     AYAGSSRATAPLTPIRLTGFRAALASLACAAPVIIGFALPAAFLAVEAYRMVLKSGIS
                     PAIVHALANSVMLSAGAAAIVAAVGISVALAARLLPDKVTTLAPRIGAIGYAVPGTVL
                     VIALLPVFAVVDNGLEVAGLSLALSGGLAAVLTAYVVRFTGMGIAQAEAGLSRLSRNI
                     DHAARMLGAKANRLAFTVLMPNILPAVGLGALLVFVDAMKELPATLLLRPLNTETLAT
                     SLYAHASAGSFEDGAIEGLLLLLIGLIPVSILARARSA"
     gene            3290..5083
                     /locus_tag="PK812_00660"
     CDS             3290..5083
                     /locus_tag="PK812_00660"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HRJ68091.1"
                     /translation="MKDLLQRHKGLLGGAVITVTVAAVLAFVPWTAPESWRETLTRRL
                     ALSLGAPELTATGAHFSLLPSPRVSLTRVSFAQNSGTVRGSAEEAVVDLSLLDLLAGR
                     VKPAALTLRRPDLTIQLPEQSEKGFRSAIELVSQVGGSLLLGQVSAPLPRLDIDGGRI
                     ALSRGPHGPAQTFDDARLRLRLPNLRSPLAVTFRARQGQTETRFSFSGATPARTLANS
                     REPITFAYSGQGITLKFDGEGIVSNAPSLNGKLSAGTTAAALGALGFPAVGKPAERVT
                     LNALLDYSNRGANLADLKIAVGEELYDGVGSVRHDGQRWHVSGTLATAKADMSTLLSP
                     ITALRKPDGRWNDTALDTDALFASSIDLRLSADQMLVFQRKLEKVALSVITRQSKAEV
                     TLGDASWHKGAVKLRASAAPGSFGVDLKLSASLEKADFGAISGDFLRERRLSGQGNAN
                     ITLETSGRSIAQFITNAEGRFAAILRNGELHGIDLNRLASRRASRGDLALTEALGGRT
                     SFETANLSAKISNGAAQPVDGHMRAGRLVGTLQGSVDFGQAEHDLSGLVVQTPADAQA
                     AEPASVLEFSIQGPLLEPRITPNVAALLRRS"
     gene            complement(5110..5910)
                     /locus_tag="PK812_00665"
     CDS             complement(5110..5910)
                     /locus_tag="PK812_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRJ68092.1"
                     /translation="MSGRLSRFDLVRLTLALAFLYVPILLLVLYSFNASRLVTVWAGF
                     STQWYGSLLRNQALLDSAWMSLKVGIASATIATVLGTLAALALARSGRFRGRAVFAGM
                     VYAPLVMPEIITGLSLLLFFVAIGMDRGFWTVTIAHTTFTIGFVAVIVQSRLAGFDRS
                     LEEAAMDLGAPPLQTFLLVTLPLIAPAVGAGWVLAFTLSLDDLVIASFTSGPGSTTLP
                     MRIYSQVRLGVTPEINAASTLMILVVVIIVLAGSLLAKFGGRNTVAPA"
     gene            complement(5907..6800)
                     /locus_tag="PK812_00670"
     CDS             complement(5907..6800)
                     /locus_tag="PK812_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRJ68093.1"
                     /translation="MSLRRLFRHVAALPPALWLGAFFALPFLIVLRISLSEKVTAQPP
                     YRPILDLSAGWEGIGTFLSQLNLFNYSTILDDELMWGAYVTSLRIAALATLLTLLVGL
                     MLALPMARAPRRLRPLLIMLLVLPFLTSFLIRAYAWIAILKPEGLLNGLLMSLGLISE
                     PLIVLNTETAILIGIVYSYLPFMVFPLYTSLEKMDASLLEAAADLGATPLKSFFLVTV
                     PCIWPGVLAGCALVFIPAVGEFIIPDLLGSSETLMIGRALWNEFFANRDWPLAAAMAI
                     CLLAVVLLPVIWQQRQAETTR"
     gene            complement(6806..7975)
                     /locus_tag="PK812_00675"
     CDS             complement(6806..7975)
                     /locus_tag="PK812_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ68094.1"
                     /translation="MAALPQSVGGNASGAAPLVRIDKVVKRFGAFTAVDGVTLDVGQG
                     EFFALLGPSGCGKTTLLRMLAGFERPDEGAILLDGANISDVPAYRRPVNMMFQSYALF
                     PHLSVEGNIAFGLKREGMARERIAERVAGLLRLVRLEGLGSRKPHQLSGGQRQRVALA
                     RALAKRPRLLLLDEPLAALDKKLREETQFELKEIQRTLGLAFIVVTHDQEEAMALADR
                     IGVMERGKLAQVGAPAAIYERPRNRYVANFIGDINILECIVESVSKDMAVLRRKPGGE
                     RILLAVPDSESEREVLESQLQIGKTTALCIRPERLELSAEAPDGATGTRSRLVGTIAG
                     TAFLGDQTAYRVALDGGAVLRVTVSNTRGAAGSALQHGTRVVVTVPADAGIVLQG"
     gene            8095..8289
                     /locus_tag="PK812_00680"
     CDS             8095..8289
                     /locus_tag="PK812_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68095.1"
                     /translation="MTAPDGGAKRPSGIHSEPGASEDNPSANLKCKHRQNCYNVEKPE
                     QVVRVYQDIAQNFYTATLKK"
     gene            complement(8329..9018)
                     /locus_tag="PK812_00685"
     CDS             complement(8329..9018)
                     /locus_tag="PK812_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRJ68096.1"
                     /translation="MQTLASIPFFKDATDIDLKNFDRRCTWRKLEEEQVVVDFEDKSS
                     DVYFLISGDVRILVRTQGGKEVILTDMHAGSYFGELSAIDGTPRSANVTALTRCEMCI
                     ISGSVFREIIFSSKVLAEKLLRLLTQRVRDLNGRLIENTVLDVRHRLYSELLRQSQPR
                     SGNPIERIVSPPPFHHVLAGRVGCRREQITRELTNMVGDGLAEKTRGGLVLKNPTVLR
                     ERIQSALKEAG"
     gene            9181..9711
                     /locus_tag="PK812_00690"
     CDS             9181..9711
                     /locus_tag="PK812_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012758151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HRJ68097.1"
                     /translation="MPLYSLDGISPIVPAPGDYWIAPDAHVIGRVRLARDVGIWFGAV
                     LRGDNEEITIGEGSNVQEHSMLHTDMGFPLTVGRGCTIGHRAILHGCTIGDGSLVGMG
                     ATVLNGVRIGRNCLVGANALVTEGKEFPDNSLIVGAPAKAIRTLDDRAVTAIAAGSAN
                     YVQNWKRFASSLKPIS"
     gene            complement(9721..10329)
                     /locus_tag="PK812_00695"
     CDS             complement(9721..10329)
                     /locus_tag="PK812_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012318528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HRJ68098.1"
                     /translation="MKLFYSAASPFARKVWAQALENGLGDRLTVVPAAVSPVSRDKSI
                     VDVNPSGKIPTLVTEEGEAVYDSRVICEYLDSIASGAKMFPAGRGRWKALVLQSLADE
                     ALDAALLARYETVLRPEHLRWPEWRAGQMDKITSSFDTLEQNWTGYLAGHVDIGVIAA
                     GCLCGYLDFRFPDYDWRKDHPKVAAWYESFSTRPSMVATAPK"
     gene            10490..10581
                     /locus_tag="PK812_00700"
     tRNA            10490..10581
                     /locus_tag="PK812_00700"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10524..10526,aa:Ser,seq:gct)
     gene            complement(10937..12559)
                     /locus_tag="PK812_00705"
     CDS             complement(10937..12559)
                     /locus_tag="PK812_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRJ68099.1"
                     /translation="MIRLESISKQTSTRMLFIEASAALNRGEKVGLVGPNGAGKTTLF
                     RMITGEEAPDEGNVSVDRGVTIGYFSQDVGEMAGHSAVNAVMNGAGPVSSVIAELKAL
                     ESAMADPAQADRMEDIIVRYGEVQARFEELDGYALDGRAREVLNGLGFSPEMMDADVG
                     TLSGGWKMRVELARILLMRPDVMLLDEPSNHLDLESLIWLETFLQGFDGALMMTSHDR
                     EFMNRIVNKIVEIDGGTLTSYSGDYAFYEQQRLLGEKQQQAQFERQQAMLAKEVAFIE
                     RFKARASHAAQVQSRVKKLDKIERVEAPKRRQTVAFDFQPAPRSGDDVISIKGVYKRY
                     GARVIYEGLDFHVRRKERWCVMGVNGAGKSTLLKLVAGASTPDSGTVAIGASVKMGYF
                     AQHAMETFDGEETVFESLERAFPQAGQGSLRTLAGCFGFSGDDVEKRCRVLSGGEKAR
                     LAMAHMLYDPPNLLVLDEPTNHLDIATKETLIAALSQFEGTMLFVSHDRHFLAAMSNR
                     VLELTQDGVHIYPGGYTEYVARTGHEAPGLRP"
     gene            complement(12756..16073)
                     /locus_tag="PK812_00710"
     CDS             complement(12756..16073)
                     /locus_tag="PK812_00710"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HRJ68100.1"
                     /translation="MRVVRAIATLLVGLMLVGATEGRAQDDQRSVLAKVISTALSSPE
                     GQVRIGAVDGPLSSNAQIRDIEISDREGPWLRVDSARLVWRRAALLSRRLEVEQLEIG
                     RIEVLRKPVPAVRQAAGNDEPLLPELPVKVVVAAFSVGELVLGAPIAGSPARLAVTGN
                     LSLGAPSEGLQAAIALRRRDRDGTSDLRLQFLPKGETLDLVLQHQESPGGLVAHVLDI
                     KDRPAVSLDLQGRGPLDAWRAQLAFSAGDAGRATGRATITRQGASRRVELNIAGQVHG
                     LLPPVAATIFSGETSITGQLDLADDGAIALPGLRLASTQAELTVAGTLGSDRRLDVQA
                     NARALPGDRTSTPRLAALSGLMLDVRARGTLDAPDIDGRIEAEISRLEAFADDLGLSI
                     RGGAKISAAVKGRPLDRTLDASLAVIGRTLTFGNAQLTALLGSQAALSGKASLRGDRV
                     AIENLALKARRVDAVLSGSVETAASATRGAITLAGKLDGRALSGQTSFVAERGGALDI
                     ASLDLRVAGGKVAGALKVAVGGLVTGAVSVDFPDLGQFSAALGTRLRGVLSAQATLTA
                     PQGAQQAAIKAKGRGLSGFGAALASLDGEAMLHGDLARPAGTLRATLAGLQYPDLQRA
                     GVPRLDIATTGALMGDRVRLDASVRGGGQIALKVSGDAPFSTRGGLGLKVTGTVDAAL
                     ANSRLTGGAQRVAGRVALDASVRGTLAEPRFEGAAVLSNGSFSDLLQGFRLTNVSGRI
                     AGAGTSLRVENVTAVTPGEGRIAVNGTVHVDPARGFPANLRVTGENARLLHNEVVRLV
                     AGLDVAVTGPLASAPVVAGQVNVVSFDVTVPDRLPVDSAPLANAKHLSPPEQTRERLR
                     LAEREGQRKARRKAGAAGNARLNLRIVAPSHIFVRGRGIDAELGGDIALSGPLLAPRA
                     NGAFELRRGQLSMLTQRLEFSRGRVTFLGDLIPDLDFTAATLAGSITAKVSVTGRADS
                     PSFALTSEPQLPPDEVLSRLMFEQAVGSLSPLQAVQLAQAVARLTGRGGPDFLEKTRQ
                     ALGVDTLDVTAGKDGPTVGASRYITRNIRLGVKAGATPEQSGVTANVDVGRRIKLQGE
                     TGASGKTSVGIAAEIEY"
     gene            complement(16073..17809)
                     /locus_tag="PK812_00715"
     CDS             complement(16073..17809)
                     /locus_tag="PK812_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HRJ68101.1"
                     /translation="MLKRLQQEPPVDGLDLVRRAEADLPKLTDLLWGEGYYSAKVTVE
                     VAGERLFLHGERQLAAARAAERARGSRIVPVRVIVASGPRYAFRSVVVRTDKGRDAGD
                     VPTKLYHHLAGAPAASAGIVEAATRIGAHYRKLGHPFASIEDPEPVIDHASRSVDVVL
                     VVRPGARANIGAVHVSGADGIDAGVIRSFIYAEGEMLFSPERMTEIRRSVARIEAVGS
                     VRVQPRDKLDGDGTVPVDVQVGVRPRHVLGGSARYSNVDGPGIGLYWADRNMFGGAER
                     LRLGADLFYLPNTYSRAWRNRFETNNLGGRLEAGFLKPALGGSRVDFLADAALSRAAT
                     EAYVSRIANVTAALRYRFADKTWVQAGAMAEFGQTQDVFGRLTYQLVGLPLSANWDTT
                     DNDLDPTRGFRLSGSVMAAYGAGDIGRFFTVSKAQASAYYALDQDARVILAGRVGLGA
                     ASGASVSEIPANWRFFAGGGGSVRGYEYRSIGPRDTLGRLVGGRSLFEASLEARIRAT
                     ETIGIVPFVDVGAAFSSAAPSFDETLRFGLGVGLRYHTAIGPIRVDVATPLDRRRGEK
                     PVALYISLGQAF"
     gene            complement(18514..19722)
                     /locus_tag="PK812_00720"
     CDS             complement(18514..19722)
                     /locus_tag="PK812_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018066246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HRJ68102.1"
                     /translation="MKITGVETYLMQVGARPSAANETKGIYADFRGSRNWLFVKVTTD
                     EGIYGVGECSGWPRVIETAVKDYANLLFGEDPADIDRLWNKLFIGSMGHGVLGTVGGG
                     ALTGIEMALWDIKGKVLGQPVWNLLGGKFRDRIPVYGHAKTPAQAKALLSAGYKAMKV
                     SFTGAVDVSKVAAVRDAVGPEIDVMVDAHGPSWATPQDAIQIGRALEPLDVLFYEDPI
                     APENIDAFQRVRDNVGVPLAAGERLSTIWGLRPLIERELVDVVQPDTGRAGGITQMRK
                     IAAMAEAHYITMAPHSGSLGPVAEFAALHVMATIPNALMLERMEFDWPGRNSVISAPP
                     VLEDGCLRLPTTPGLGVDIVPEEIARHPSVRNVSVLPDDDGWAYEAGTVDEAVYFQTR
                     LQRRLKLKFT"
     gene            complement(19819..21555)
                     /locus_tag="PK812_00725"
     CDS             complement(19819..21555)
                     /locus_tag="PK812_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010973628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ68103.1"
                     /translation="MSAAPPVIEVKNLVTEFATRHGPFRAVDDVSFTLRRGKTLCLVG
                     ESGSGKSVTARSILQIVDPPGDIKSGKVLLHRHNTARGRQSGESVIDLAALNKRSSAI
                     RAVRGRDIAMIFQEPMSSLSPVHTIGDQIGEAIRLHENVSKKEALNRAIELLRKVEIP
                     NADKTIHRYPFEYSGGMRQRAMIAMALACNPGVLIADEPTTALDVTTQAEILTLIRKL
                     QDDNGMSVLFITHDMGVVAEVADEVAVMRYGKLVEQGDTLPIFEEPQHEYTKMLLGSV
                     KQLDRPSTRRLARRASNPPGDVLLSVDALRKEFHIDRGSFFRKTAPLVAVNDVSLQLR
                     SGENLGIVGESGSGKSTLGRAILRVYDTNGGSVNYTGKDGVTRDLVTLDENQLKSARR
                     ELRMVFQDPMSSLNPRMTVAQLIGEPMLVNGMLQGRALNDRVAELLQMVGLPASAMER
                     YPHAFSGGQRQRIGIARAIALDPRIIIADEATSALDVSIRTQVLDLLLDLQERLDLSF
                     IFISHDIAVIRYFCDRIAVMYRGRIVEEGPTEEVCNTPKHAYTQALLSAVPHPDPRMR
                     GTRVRTRYREPA"
     gene            complement(21555..22682)
                     /locus_tag="PK812_00730"
     CDS             complement(21555..22682)
                     /locus_tag="PK812_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018063979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ68104.1"
                     /translation="MVIDTRQHASTDQASQWKLMWIAFRRHRLAVFGLIVVGLLYFIA
                     AFAELLAPFDPDASSARAVNHPPQIVRFIKTTPDGGWTFQPHVYAMAQKRDPITLATT
                     FTSDPSKTVDIEFFGKGAPVRMWGTFEVERRLIAPKKSDERFYLIGADRLGRDMLSRI
                     IYGTRISMSIGLVGVLMSLVLGVFLGGISGYFGGWIDASVQRIVEFKLAMPTIPIWLG
                     LSAALPQDWPPTWRYFAITVILSLIGWTELARVVRGRFLALRTEDFVTAARLDGCSQM
                     RVIFRHMLPSMVSHIIASVSLAIPMMIIAETSLSFLGLGLLPPTISWGVLLKEAQNVR
                     TIAEAPWLFSPGVAVCIAVLALNFLGDGLRDAADPYAQEGR"
     gene            complement(22693..23676)
                     /locus_tag="PK812_00735"
     CDS             complement(22693..23676)
                     /locus_tag="PK812_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018066249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ68105.1"
                     /translation="MLVYVLKRLFWSIPFLFAVSLVAFALIQAPPGDFVTSLAATLAA
                     SGDVVDQARLDALRERYGLNESFLVQYWRWISGVLRGDFGQSFEWSQPVSDLIWERMG
                     LSVAMAAATLLFTWAVAFPIGIYSAVRKYSIGDYVISAIGFIGLSIPNFLLGLVLMYV
                     SVVWFSHDASGLFSEQYQQAPWSWDKFVDLCKHIWVPVIILGTSATASLIRIMRANLL
                     DELHKPYVTTARAKGLSEWKLLVKYPVRLALNPFVSTIGWAFPQLVSGSVIISYVLSL
                     PTAGPLLLQALLAQDMYLAGAFILLLCCLSVVGMLVSDILLALIDPRIRYR"
     gene            complement(23739..25661)
                     /locus_tag="PK812_00740"
     CDS             complement(23739..25661)
                     /locus_tag="PK812_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007690395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRJ68106.1"
                     /translation="MAHIRRALIVAALATGVSTLAVGTAASQGAKSEAPALAALVKDG
                     KLPPVANRVGDEPEVVTPLKSVGRYGGVLRYGLRGSSDFNNILRMTGPQGLVRWDLGY
                     TKVIPNVAKSFEVSPDAKVFTFTLRKGMKWSDGKPFTADDVLFNMNDIVIAGALTPLG
                     PRYMAGGKPFTVEKINDTTVRFSFAEPYGDFLAELASPLGQNPVLYAKHYCSQFLPKY
                     NDKIDDLIKENKQADWKGLFQSKCGDLEISARWGNPARPTLDPWVVKDPYVGGATRVV
                     LDRNPYFWQVDTAGNQLPYIDQLVSPIAQDVESLILDAIGGKIDFQLRHLDSPANRPV
                     LAQNRDKGGYEFWTANAPGGSNMVINLNLTHKNPDLRELFNKKDFRVALSIGMDRKEI
                     IETALLGQGKPWHQGPWEDHPNFHRKISTQHLDFDAAKANQLLDSVGLNQKGPDGMRM
                     WKGKPLKFKIDVIPTLQPEQVDMLQVIKRQWAKIGVDMDVNAVERTFFYQRTSESNDH
                     DAAVWGGQGSWVPGDIPQQIVPVHHDSRWGIPWVNWYKTGGKSGEEPPASVKERMKLY
                     DEARGTADPAKRKALIDKVADIAAEQFEVMGVATSAPTFGIRKKTIRNAPDAMVSSWY
                     WPTPAPSLLQQYYYAN"
     gene            25751..26734
                     /locus_tag="PK812_00745"
     CDS             25751..26734
                     /locus_tag="PK812_00745"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010974020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HRJ68107.1"
                     /translation="MKLATQLQARDDSDFEVWSQLGITHLCQDPPGNPHDWTLSDIER
                     LRDTIGRYGMVHDMVQLPLSSRPIEQAQSPDILLAGPERDRQIDSICDLIRNLGRAGI
                     PAAKYNLNIIGIPRTPLEAGRGGAKAEAFRWELTDQNAPPGLAGLLDADTNWERIDYF
                     LERVVPVAEESKVRLACHPHDPYTPPGYKGVTRVLGTVEGLKKFVTMRESPYHGLNFC
                     QGTVGEMLEDPGTEVFDVIRWFGTRNKIFNVHFRNIAGKRLSFRETFPEEGTMDMWKS
                     MKLYREIGYAHMIMPDHMPHVSGPNEVGVAFAFGYGYIRALIQAAEAEHAA"
     gene            26724..27605
                     /locus_tag="PK812_00750"
     CDS             26724..27605
                     /locus_tag="PK812_00750"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HRJ68108.1"
                     /translation="MRPEIVVAADVRCETGEGVFWDAETQALWFVDIPVGRLYRLNDG
                     TLHHWDMGEPLGCLAIDRAGNPVVALKHDIVRLDLSTGGRVLLARPETDRPHNRFNDG
                     AVDRQGRFWAGTMKMGQPAERTGAFYRIDADHRVTRFWDGTFTTNGIAFSPDGRTMYL
                     SDSNPEVRTIWACEYDTDTGTPGERRVFFDTRAVAGRPDGATVDADGCYWMAGVGGWQ
                     LLRITPKGAIDRIVDMPCERPSRPWFGGRNLDILYVTSIGAGLSSGTEARQPHAGALF
                     AVHGLGVTGLPYPRFCG"
     gene            27622..28560
                     /locus_tag="PK812_00755"
     CDS             27622..28560
                     /locus_tag="PK812_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018238410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRJ68109.1"
                     /translation="MKTSPVTVRDLRRSVLAVPPLCRDADGMVSLAENRRLTAHLRAG
                     GVTTHMWGGNANIYALGVSDFPAFLDVAEACAVGDEWALPSIGAEFGKAMDQAAILSR
                     RAFPTAMILPMRGVVDRRGAARGIGMVAAKMGKPLIVYYKDSGYLDMADLKALFADGS
                     LCALKYGVLTPDAAVDPELGKILKAVPPDLVISGSGELPVVAHAETFGLRAFTSGCVC
                     IAPRLSMAILAAIQSGDFARARAIRETFLPLEHAREQHSPIKVLHAAVGLAGVAATGP
                     LQPMLSELTEPAVLDALGREAKALLAANETMKVAAE"
     gene            28746..29900
                     /locus_tag="PK812_00760"
     CDS             28746..29900
                     /locus_tag="PK812_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018858153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HRJ68110.1"
                     /translation="MIITDVRVTTFRHTVHTFKDEDGHTHPGPPRQVLSSLLTIATED
                     AEGHVHGPTELLRPFIVETFLKKVLLGQNALMREKLWHGLAHWQRGSASMLTDRILAL
                     VDLALWDLAGRALGVPVYKLIGGYRDKVPAYASTMCGDEIEGGLKTPDDYARFADWCK
                     NKRGYKAMKLHTWMPPVTFAPSVDMDIKACAAVREAVGPDFPLMLDASHWYSRQEALR
                     LGRAVQDLGYTWFEEPMEEASISSYANMARELDIPVIGPEVADGKYFTRAEWIRAGAC
                     DILRTGVHDVGGLTPALKCVHLAESFNMACEVHGVGAGNLTLCAVQANGRWYERGLLH
                     PHFDYDATPDYLHEPEDAMDADGFVHLSQKPGLGAHYNWDFITANTVSTH"
     gene            29926..31095
                     /locus_tag="PK812_00765"
     CDS             29926..31095
                     /locus_tag="PK812_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003505685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HRJ68111.1"
                     /translation="MKIVRLRSFMSRDANRPRVLVAIDTDEGLTGWGECYNHGPDRAL
                     PDLLDYYFGQIVGHDPTRIEFLYLKLLQHNRFPPGALGLAAMSAIDHALWDISAKALN
                     VPVYQLLGGRCRDKVRVYSGVYTAPDPEAAKDQLGKLNADWGFTAFKLSPYRIDHHSN
                     RWGEVVRTSAEYFRELREVCPAHYEIAFDAHAKIFEVQQAIQLGNALAPYDPLFFEEP
                     IRPEHMPAWGELKRGLTCTLATGESLYNRYEFLNLLSHKGCDIVQPDICVVGGISEMR
                     KIAAIAEAHFVTIAPHNPMGPLATAVNVHFSAATPNFRILEYRLPTQRYMGTDDSDEG
                     CYVADPYLPKDGFLELRPDRPGWGVEMDMKYLSSDRYIHWERKLPTRPDGSTAFM"
     gene            complement(31237..32871)
                     /locus_tag="PK812_00770"
     CDS             complement(31237..32871)
                     /locus_tag="PK812_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006934091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HRJ68112.1"
                     /translation="MSKTVNVLMVFPRFTSTSFWSLKTTADVIGKVWLSPPLGLITVA
                     ALLPAHWRIKLVDCNVEDLHDADVRAADVVMTGGMIPQRAACLALIDRVHALGRPVVV
                     GGPDVTSSPTVFEGAEFRITGEAEAVLADFVAAWERGERTGRFDAEKYTVDVTRIPVP
                     RYDLLKIDRYSSISVQFSRGCPFTCEFCDIIELFGRVPRAKNPEQVLAELEALYRLGH
                     RGIVDFVDDNLIGNKKAVRVFLARLVAWQQAHGFPFEFFTEASINLADDEDFLTLMKA
                     AGFNLVFVGIETPDPATLVAARKKQNTRRDLADSVARINRAGIQVHAGFIIGFDSEPA
                     SIAADMIACIEQAAIPVCMIGLLTALPNTQLSRRLITEGRLDPVTDLDFAAATAHDGT
                     SDQCTAGLNFRTLRPRREILKAYREVLDRIYRPEAYFKRVEIMAIRLGGKTPAPRLEL
                     SRLRGDLRAVFGLVRQLTVRQPEHRRFFIRTLARVIVNNPSAYRFAIGLMVPYFDLAP
                     LARQVIQWLDAEIATLAPDVAGPVEHVEGLKAVRAS"
     gene            33071..34204
                     /locus_tag="PK812_00775"
     CDS             33071..34204
                     /locus_tag="PK812_00775"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRJ68113.1"
                     /translation="MKQAVNAALSYAEGWLRRPTPIADPVILDIVLTKACNLRCSFCI
                     SYASLAQTHWLDYGLYQRIAAELFPTAWDVQFCSGGEPFLYPQIREALQLARHHGCKT
                     GVTTNAMLIDDRVATWLVEDQTLDRMWVSFDGGTKETLERLRLGARFDKIIANVRNLV
                     AKRRAAGARFPMVDMRFVMMRSNVAELARLIELAADLGVRRVEGRYLNVANDMDRDES
                     LFHHRGLAAEEFARARAVAEAQGVRLSLPPLPGEDRGRHRCIKPWEMCQIDVDGSIRF
                     CYKSWRQRLGNFDDGFSNIWRGEHYQRLRATMDSTAPYFPYCRTCAMRIGIDNESAHD
                     QSSHAEDYVIPGLEHWQTDFNARAEENRDSFAERKDRLRGKKA"
     gene            complement(34213..34611)
                     /locus_tag="PK812_00780"
     CDS             complement(34213..34611)
                     /locus_tag="PK812_00780"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HRJ68114.1"
                     /translation="MGLDLKPGDPLPVLPLAAVSREQVLAYVTCAGDDNPIHVDEAAA
                     GAAGLAGLVLPGMLIAAQFPRLLQLWKRNIRLTDITVQFLAPLGIDQAFVVTGKVVAI
                     DRNGAAAIVRMTARAGTANVAMAEARIVQE"
     gene            complement(34611..35036)
                     /locus_tag="PK812_00785"
     CDS             complement(34611..35036)
                     /locus_tag="PK812_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68115.1"
                     /translation="MPADDDLRPIACFEVVAASQHVSGLRHAVALPLVSGEAPAMPEV
                     PATYPVIWLGNPEIRAAVRSVAGMEKGLPLHFLQSVAYFSRIEVDGTYALDLSWRPLP
                     HRDPMRPKLEFVAIVADAAGKMVARMSSVLLVVPAVRGA"
     gene            complement(35047..36258)
                     /locus_tag="PK812_00790"
     CDS             complement(35047..36258)
                     /locus_tag="PK812_00790"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HRJ68116.1"
                     /translation="MRQVAITGMGAACATGLGVERLWTACRDGIPALGPVEMTRPCKL
                     RVDRAGQVRDFDPLQHIAPAMMSRCDRFTQLAHVAAREAVAQSGLSAEELAGTRTAVI
                     IGTGVGGFTSLDQGHYQHYVENRRADPMGIPRQMPSAASSFVSMTYGATGPCFAVTSA
                     CASATQSIGIGAMLVRSGVVDRAIVGGSEAAITPVAMRSWESMRVVTSDQCRPFSKGR
                     SGMIVGEGAGVLVIEALEAVRARGGMPLALLAGYGTSSDARDFIQPDVAGATAAIRSA
                     LADAQLDPASIGYINAHGTATVLNDVNEAAALRNVFGAALDAIPVSSSKPIFGHGLGA
                     AGGLELIVTVKALVEQTVPPQINFLEPDPACPLNLPTQGAFPREIGAALSNSFAFGGI
                     NAVLVVTRADL"
     gene            complement(36255..36566)
                     /locus_tag="PK812_00795"
     CDS             complement(36255..36566)
                     /locus_tag="PK812_00795"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRJ68117.1"
                     /translation="MINEKPLMLEWPAMPDQEMLGKIIDVVAKVGNLERDRITPDATL
                     DSLGLESIDVVTILMDLEDVMDTYIPMNSDLASARNMAELVNEAVREMNKAAKGSEAQ
                     Q"
     gene            complement(36623..37768)
                     /locus_tag="PK812_00800"
     CDS             complement(36623..37768)
                     /locus_tag="PK812_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68118.1"
                     /translation="MSVIVAPVNSPRDLALFIDLPRLLYRGDRRYVPPLTLDRRQNLD
                     PRKGAFFTHGEAQYWLAFRNGTPAGRISAQIDHAATASEFAGTGFFGCLDAIDDAEVV
                     KALMHEAEAWLRARGMKRVRGPCLLSMNGEPGLLVEGQGEPPMLMVPWHPEYLLRLIE
                     ACGFGPVRDLFYFKIAIGTAELEEVSRRLRLPKWRLNYTIRKLDRRHPSREIDILREV
                     YNDAWQNNWGFIPLLEADLAAFKTQLRPFMHPDYGIIVEKDGEPLGIVLMLPNLAEMS
                     ADLGHSPSPLGWLKFLWRAKMQRFSSARVILFGVKARLSRTVEGAAVAMMLVHEMVTL
                     ARRYTLPAIEAGWVLDSNRPLIAILEQFNARRVRTLRLFEKDIQAEK"
     gene            37931..38716
                     /locus_tag="PK812_00805"
     CDS             37931..38716
                     /locus_tag="PK812_00805"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRJ68119.1"
                     /translation="MLRHIVITGASSGIGRALAEHYAGPGVMLSLTGRNAERLAAVVR
                     GCEARGAMVASSLIDVTDRVTLADWLESRDTVQSVDVVVAAAGIGGAEVVPEARGESA
                     DLAGRIFATNTLGVVNTVAPLLARMVARRTGAVVVIGSIAGRIGLPQSPAYSASKAAV
                     HIYADGLRRLVASSGVSVTSVVPGFVDTPMSRSLAMSRPFCWSADRAARRIARAIERR
                     ERRCIFPLPLRIATALHHFVPLALLDRVLGRANTVIRPGRADP"
     gene            38713..39726
                     /locus_tag="PK812_00810"
     CDS             38713..39726
                     /locus_tag="PK812_00810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRJ68120.1"
                     /translation="MTSKRFFYLVPAETRHRLVTETRLQHLRRLLFDRRRRYPTGGVK
                     VIYQHCEMLLDAGFTAHPVHLGDFVIDWYDHQCRPLSVAEARAMMRADDVLVVPERLP
                     AVAAQFTCGTKVAFVQNHGLVAAALQGRRYADFGFTDLLCCGTFVAGTLADQAPLPCH
                     VVTNGIDLDKFVPAPDLRRPDTVLILRRKASWPLGLQAIGRLPQEIRTRLAVRVLPNT
                     ASETQMIRRYQESDIFLALGFPEGFALPPLEAMACGCVVAGFAGGGGISHMRHETSAL
                     VVPDGDVTALAAALERLAREPDLKERLRAGGQAVAQGFGLAAMRAAVLAFAETMQRRM
                     TSS"
     gene            complement(39716..40918)
                     /locus_tag="PK812_00815"
     CDS             complement(39716..40918)
                     /locus_tag="PK812_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HRJ68121.1"
                     /translation="MGQTDSPYHTIGDYAQRLFRFGGGHELGAARNFYFKAMDEAKAA
                     AEARGQTFVSFANYDYLGLSRHPDIAEAGIAAIREHGSGALASRLAGGERTVHGLLER
                     DLAEFVGAESSLALLSGYLTNETMISTFFVGRDLVLCDEFSHASILAGTRGTRAKTLA
                     FRHNDLEHLNALLTEHRAAHANCIIVVEGLYSMDGDVPDLVRLLDIKDRHRAWLMIDE
                     AHSHGILGETGRGICEHAGVDPARVDLIMGTLSKTFATCGGFLAGSRALIDVLRFGLP
                     GFLFSVGIAPAVAAMARTAISLAKQEPWRPQAAARKAERLLMRARAAGLNTGDAIGRA
                     LVPIIFRNQQETVAAAERLMDAGIYAPPIVYGVPRNMPRIRLFVTAAHDDADIDRVVD
                     ILAQSVRS"
     gene            41030..42088
                     /locus_tag="PK812_00820"
     CDS             41030..42088
                     /locus_tag="PK812_00820"
                     /inference="COORDINATES: protein motif:HMM:NF024918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HRJ68122.1"
                     /translation="MFVVSGTIVARILYGVQGDAGGHVSRSLTIARQLHGHEVVFVGG
                     GRVAQARRHGYDVVDVPMLANVYTNNRLDLPATLMAGARALRDRARIFDRLTSLIRDF
                     DPDLILTDYEPFLPQAARLAGRPSISINRQHFLTHCDYTVPSGAQLARLLAMQALRRF
                     HMSASQYFIVSFTKATAKEPKTAEVFPSVIRPEVAVRSGSDSGAVLAYLPLASPDWMR
                     RTFAGLDRKVIVYGQNRDGDVGNLSFRPRTSDGFADDLAQSAFVVSHAGSNLIAESLF
                     LKKPVLTFPTARIFEQYLNAHLLAGAGYGKIGSQRNSAASVAAFDRELHLYRSRLEQY
                     RPWRDTTVVDRLSGLLAR"
     gene            42256..43464
                     /locus_tag="PK812_00825"
     CDS             42256..43464
                     /locus_tag="PK812_00825"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HRJ68123.1"
                     /translation="MSALAAHVATVAKLAPSLLAHPGYSYETAKLLLSRLTLAMKTPA
                     DYRGGQIRQVSIRITDLCNLRCHTCGQWGDNGYLLGQPMRELLRAEVPVDRYIALLHD
                     LAARGHRPGLYLWGGEPMLYRGAVCLIEEAAKLGMPTSIATNGTKVAENAEALVRAPM
                     FIAQVSIDGHNSDIHNACRPGANPSTDNFSTVVGALDALKAERKRQGGKLPILAGLCT
                     INQRNADRLVDIYDAFHDKLDFMIFYLSWWIDAPSAAAHTTEFQRRFGTRPVRHEGWI
                     GGWRPTDYSGLSRQLATLARRALSPRGPGVLLVPDLTSPEDLERYYTDHGATFGFDRC
                     TSIYRAVEINANGDVSPCRDYNDYVVGNIKTHTITDLWDNDAYRAFRRSLKCDGLMPV
                     CTRCCGMMGN"
     gene            43527..44744
                     /locus_tag="PK812_00830"
     CDS             43527..44744
                     /locus_tag="PK812_00830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRJ68124.1"
                     /translation="MLKTAYILLWFPVPTETFILREVVNLRRYGLPVFVFSLYGRWRK
                     NLSREMSEQAADVERLGMWSLPRLIAALVHWLWRKPGETLHILRRFTFRRWRGLEKTG
                     ENLWALLAGFHLARRFEDLGIEHIHAPWAAGPATAAWVASQLTGRPYSFTARAWDIYP
                     ADALIGEKVDAATFVRTETSANVAHLKAITGAADAKFRVTYNGMPLTRTVDAPLRMTP
                     PYKLLAMGRFVPKKGFDVLVAAAGILAGRGVPFELTFAGDGRLRNALERQARALGIAD
                     RVRFPGFLSHDSVGAAFAGADVFVMPCVVAASDDRDGIPTVLMEALMHRVPVISTPVS
                     GIPELIRDGETGLLVPERDPVALADAIQRCLTDRDFSVRIAEQGREQVRRQFDAERNH
                     RAVLALYATIGRP"
     gene            44744..45823
                     /locus_tag="PK812_00835"
     CDS             44744..45823
                     /locus_tag="PK812_00835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRJ68125.1"
                     /translation="MAAAGRPLRLVLLLEDLDAGGTQRYALYLATHIDRSRFKPEIWT
                     LRGGSTFAPLAAAAGIPVSDLTRSRRVGPVAVLRLIWRLLFRRPDILYTLTIIPNIWG
                     RLFGGLLRIPVVSGYRSLRPAQLDRLLHPLSRRIIANAPQLKDVLTDELGVPAARVSV
                     VPNGVDLDRFSPVAHHESETPLIVCVARLVPPKDLPTLVAAFAIVRAALPEARLTIIG
                     EGPVALAPATQLSVLPARSDVPAVLARAWVFALSSIEEGAPNAILEAMASGLPVVATD
                     AGGIRDVVEDGMTGVLVPRRDPQALATALLDLLRNRDTRRRMGEAGRRRAEERYSIAA
                     MVRATEALLDDAAGGAAPGSGGALA"
     gene            45820..47160
                     /locus_tag="PK812_00840"
     CDS             45820..47160
                     /locus_tag="PK812_00840"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRJ68126.1"
                     /translation="MNGPLPPTIWIEVDDIIEYFTRHDQPTGIQRVVFELLNAARQRS
                     RQAVRLCRLNRRSGRFEALDLTGYDAMLTWLRREGRVYQKYGRLHARLGIWARDLYRL
                     RHWRDPGRKLSARHDARAETFFREIQTGAVILSLGGPWLNPRFPSAVRALKSRPGVRF
                     VQLVHDIIPISHADLLDARAIPQFVTWLHETAPLVDFYLTPSRHVAGELQSYAAAQGW
                     PRRSITVIRFGSGFAPAAPVGQEPLPCPVGEPYALCVGTVDLRKNTELLVQIWERLVA
                     KHGRDRVPLLVLAGHRGYRSQAVFTRLQATGFVQGRVRFLERVSNAALEDLYRRAAFT
                     VFPSKAEGWGLPVEESFVYGRPAIVSTATSLPEVGSDLAEYFDPADAEGAYARIERAI
                     TDPAHLDRLAERIRKDFRPRSWNETFDQIAEVIGRECALLERGRHDAAERGTGT"
     gene            47165..>47934
                     /locus_tag="PK812_00845"
     CDS             47165..>47934
                     /locus_tag="PK812_00845"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRJ68127.1"
                     /translation="MPALSLVVCLHREGAFLERMLAHADGCYDDLVVVHDGPDEDAVR
                     DIVEARGGRFFERPRSWQQEPHWSFAWEQARHDWILRWDADDLPSDALATWLRAFRTA
                     PEPAAEISAFTCIYPLWDGKTARTRRWPRRIGLLNRHRVRHIGMADQPPIPDGEVTPL
                     ELVLRHEPDRPSYGVWYTVFRPTSRRWHLDIARSLLGKPTDLPCWRWTSPIWPPAWEA
                     IRRHPLRTGLRRLFTTPFNNARAMIRHGEWPRPSMLVFS"
     assembly_gap    47935..48034
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <48035..48882
                     /locus_tag="PK812_00850"
     CDS             <48035..48882
                     /locus_tag="PK812_00850"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ68128.1"
                     /translation="GPPSKVTDDPARVVSVLAAPAVCLWRHRNLARRLVRRDIEARFR
                     GTVLGKIWAVIAPLLMLGVYTFVFGVLIQPQWQAAVNDRFTVPLIYFSGLILFQFFLD
                     VFSRAPALIRDNAVYVKKVVFPVEIFAWIVVGSALFRLLIGFAMLVGFHLLLKGLPPV
                     WLVMIPLFLAPLALISAGLVWFLAGLGTYVRDVSQVVQVLTPVMMFISPVFFPLSAIP
                     EPTRSVLYANPLSFPIEAVRSVLFGGGFTNWSGLAAYALGAVIVAGLGYRFFMRVRPG
                     FADVL"
     gene            48872..50170
                     /locus_tag="PK812_00855"
     CDS             48872..50170
                     /locus_tag="PK812_00855"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ68129.1"
                     /translation="MCSDVVIAATRLGKTFRVFTKPSQQLGALLLPRRGGGSELAALR
                     DVTFTVEQGESVAIIGRNGSGKSTLLNVVCGILTPTHGTVTTRGRIVGMLELGTGFDP
                     DFSGRENARLTAAIFGLGEEEIAARLEHMTEFADIGDAIERPVREYSSGMVARLAFAV
                     CAHVDADILVVDEILAVGDAAFQQKCMRFLRTFKAKGGTLLFVSHGEHAVLSLCRRAL
                     WLHQGKVLADGPSKEVYHAYAASIEEHEGMLSAPAVALGHGPAPVRAPASSPFQPFRY
                     DPDASAPAFSGTTITQIDLCDENGDRRVVLEGGEEIDVSVRCAFEEPVSSPVVAFLVR
                     NKLGRIVFADDTAQLGSAAASAGAFLARFTLPLPYLPSGDYLVEAFVFDNRAPSPQLL
                     ARSIDRCFFQIQSRHPGGGLANIAPRRIAIGPAEPGTMAP"
     gene            50167..>50786
                     /locus_tag="PK812_00860"
     CDS             50167..>50786
                     /locus_tag="PK812_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018516564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ68130.1"
                     /translation="MTGDLAIIAEGIGKTYRMYPSQGSRLADLLRPAPRRGSAFSALD
                     SIDLKVRRGESVGVIGRNGAGKSTLLQVIAGMLRPSTGRVTVNGRVAALLELGAGFDP
                     DFSGRENVAMAAAILGLSDTQIVERFEAILAFAGIGAAIDRPVREYSSGMYARLAFAV
                     AVHVDADILIIDEILAVGDAAFQRQCHRAIEAFLSRGGTLLFVSHD"
     assembly_gap    50787..50886
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <50887..51351
                     /locus_tag="PK812_00865"
     CDS             <50887..51351
                     /locus_tag="PK812_00865"
                     /inference="COORDINATES: protein motif:HMM:NF025877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wzt carbohydrate-binding domain-containing
                     protein"
                     /protein_id="HRJ68131.1"
                     /translation="QGTVWHGEGGGEIEHIALIGRDGTPLSATHGGDEVELRISCRAR
                     RALNRPIVGYILRNANGENLAGDNTYLRTRESGFPVAAGEQFVARFILQLPYLPAGDH
                     FFAPSIIEGTQRDHIHLHWIENALCLRAIASPVTLGAVGVPARNVSFGPVVG"
     gene            complement(51348..52745)
                     /locus_tag="PK812_00870"
     CDS             complement(51348..52745)
                     /locus_tag="PK812_00870"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRJ68132.1"
                     /translation="MSNARPMRLVIDGQALQTASSRNRGIGRQALALAQAMVRNRGRH
                     EVLLALSSFEPETIEPVRAAFDGLLPEHAIRVWYAKGTMPRRKPKTGERSDAATIIYE
                     AFLAGLSPDLVHMPSLFEGSGLDAVTSIGRFTRLYGTVVTLHDLIPLLDPDRYLTDPA
                     HRAWYLRKLEDLHRAEAMLAISESTRREAIQHLGCPAEKVMTISSGVDHRFRRIERDE
                     KTVRDLLARHRIARPFVMYTGGGDHRKNIAGLIRGFAGLPEALRRAHQLAIVCHLAPR
                     ELQPLARQAAELGLDPDEVVFTGYVPDDDLIGLYNACVLFVFPSLHEGFGLPVLEAMG
                     CGAPVIGADRTSLPEAIGRADALFNPDDDASLVALMQHVLTDTGFQADLAAYGEARAR
                     QFTWDACAERAWRALEQVQATRTRQTADNATLVSQAVAAIAALDPLHVRQTHKTLARA
                     LAHNHPDGAGSERTL"
     gene            complement(52742..52927)
                     /locus_tag="PK812_00875"
     CDS             complement(52742..52927)
                     /locus_tag="PK812_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68133.1"
                     /translation="MLPSLLNPKSCRICRWFARRTQQKAGVAGLSERVIAIDLALLEA
                     SCRRHGVVVLWWTWLRR"
     gene            complement(52987..54285)
                     /locus_tag="PK812_00880"
     CDS             complement(52987..54285)
                     /locus_tag="PK812_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68134.1"
                     /translation="MATPPFDDAGLPHASFAARLSAQGCPALRQPVTVPGPPVRRFAL
                     RVTARATGEGGVCRLSAVSPRPEGSDHLLHQAEFVLTERWQRCRTVFEWTADGPCVIE
                     IRAIGAAAIDVTDVRLVGLLDADAPHAVRFDTRGTMLLPSTRLRALMIEDYLHLLGWR
                     TSINAGSNYDLLVCQKTTRFAGLLAARLRGRRVVYDLDDNEFAESLLTRARIEVFARL
                     VDAVTAGSDVLRRRLSRAAGSVHLLENPVDVLDHSVVHGNLDWRGRLVWFGMPENAWM
                     LAAAGITQPVVRITRGGDVPYDLRTIDQQLVLHDLALLPLALNDHTQAKNANRLVKCV
                     GLGLPFLASDTPEHRRALEKLGLTDQVLVRPGQDWTERIAEVADDYAAFKARIVAARP
                     VAFAAYGIEAVAAQWLAFCEGVMANERHAAIRVGFRREPE"
     gene            complement(54406..55578)
                     /locus_tag="PK812_00885"
     CDS             complement(54406..55578)
                     /locus_tag="PK812_00885"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HRJ68135.1"
                     /translation="MARELAFYVAVGIVALSVPIIIISLRVHLPSFALPFERLWLMIY
                     AIASMILVHTIPEITAMAIVATYSRFLSDGAISTLFSAGRSPLAIAMPAAVVAGGMML
                     AVAVLSMAVAPGSAKNIHDTLLFLRRDFNLSMLEPGVFHEFRNEGVSIFFGRKIDATQ
                     IQDVFIAKRQPAGHPSGNREETYVARRAVVVGMEGERGLLLLEGQAIVVKAADNAVRR
                     VEYTRAFWSPDSGFATPARGYVFFDEVGTVQLLTQGPTAATQRRATELWLREVVWRMG
                     APILTLTHTLFGLGLLLTFSPISGRQRTPYLVAIAVCAAILALHILYMTLIELVARIG
                     ATGTVLFVAAIVAQAVAGILLLARSQVPLLRGRASAWGDALLRLAEGRSRVFGAKD"
     gene            complement(55766..>55974)
                     /locus_tag="PK812_00890"
     CDS             complement(55766..>55974)
                     /locus_tag="PK812_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68136.1"
                     /translation="VRFTAELIMLAGRGGPLPWRAPLACLARDIVTLALWLPAWRTRQ
                     VRWGGRGHYPQGRFSASQRNGEVR"
BASE COUNT        10075 a        18379 c        17497 g         9823 t
ORIGIN      
        1 gcctccaagc ttccggccaa cgagcgcaag gccatcgaag cgatggaggc ggcctccgcg
       61 cgcggcaccg cgctttcgcc gagcctgcgc gcccggattt cggcgcaggt gacacaaggc
      121 acgcagagtg ccggaccgaa gcagcaactc gccatgcgtc cggcgctggc ggggcgcacg
      181 tccaagggca gcagcggtca ggaccttcgc tatgtcggcc cggacgttga tttcgaccgg
      241 gctcggtaaa gcgccgactg cgacaatctg tccgagcccc gcgctgcaag ccgcaacgct
      301 cgcaagcatt gcggcttttt catttcccaa tccgcgactt gttcctctcg ctagaatgat
      361 ccgaaactgg ctttttcaaa ttagaatttc tctaagatat tgaaatcgct gataataaat
      421 tgacgtcccc aacatcgtgc cgtaggcatc gctgcataac cgaacccgga ccttgaatcc
      481 ggcccagcgg agcatgtcat gcattcgaag tcccttcgcc cggcagcgct ggctgcagcg
      541 ttgttctgca ctcctgccgt cgcgcaggag gtcaatgtct atacatatcg cgagcccgcg
      601 ctgctcaagc ccctgctcga tgcgttcacc gccgaaaccg gcatcaaggt cgccacgatc
      661 ttcgccaagg aaggtctgga agaacgcatc gctgccgaag ggacgcgcag ccccgcagac
      721 gtgttcatga ccgcagattt cgcctcgctc gaacgcgccc ggacaatggg gcttacgcgg
      781 caggtcaact cggaagccct gagccgggcc atcccacccg gctatcgcga cgcgcagggc
      841 cactggtacg gcctgacgta ccgcgcccgc gtcgcctatg tttccaaggc cagggtatcg
      901 gagacgcaac tgacctacga ggatctggcc gatccgaaat ggaagggccg tttctgcctg
      961 cgcagcggca agcacatcta caacaacctg ctctttgccg cagccatcgc ccacatgggc
     1021 gaggccaagg cagaagactg gatcaggggg ctcaaggcca acctggttcg caagccctcg
     1081 ggcggagatc gcgaagtggc gaaggacatc gcctcgggtg cctgcgatat cggatttgcc
     1141 aatacctact acatgggaca catgctcaac ggcgcccctg atcagaagcc ctgggccgaa
     1201 gcggtgcgcg tcgtcctgcc gaaattccgg aacggcggca cccacgtgaa cctttcaggc
     1261 atagccatca tgaagaacgc accgaatgcc gccaatgcgc agaagctcgc ggaatggctg
     1321 gtcggcgaaa aggcgcaggc gctctactcg aaggtgaact atgaatttcc ggtgcgggcc
     1381 ggcgttccca tcgatccggc gatcgccggt ttcggtgtgt tgacaccgga tacgctcagt
     1441 ttcgaagcta tcctcaagga gcgcacgaaa gcggctgatg tcgtcgatcg cgtcggcttc
     1501 gacggtggtc cggggtcgtg acaggcgcca gcgcagccat tccttcagct ctcggtcgcc
     1561 gctcgtcttc gggcggcgtg ctgctgcggg cagccagcct tgcgctgtat gtgctgctgc
     1621 ttttgccctt ggccggcctg accgtgctcg ccttcgccca gccggtggaa gggcatgtgc
     1681 tgtccgcgtt gcggctggcg tccggcgaga cgcttaccct gctgttgggc gtcggcgtgc
     1741 tctcggcctc cgttggcgtc ggcgccgcct ggttgatcgc aatgtaccgc tttccgcttc
     1801 gcgacatcct cgccgtgctg ctggtggcgc cgctcgccct gccaacctat ctggcggcct
     1861 atgtggcggt ggaactcacg gactatttcg gtccgctcca gtcggcgcta cgcttgatcg
     1921 gcggctacac cctgcgccgg gaatactggt ttccggatgt gcggaccctg cccggcgcca
     1981 tcctggtgct gtcgctggtg ctttatccct atgcctacct tgcggcacgg atcatgttcg
     2041 agcggcaagg cagcacgctg atccaggccg cacggcttca gggatgcacc ggtctgcggt
     2101 tgttcctcag gataggcctg ccgttggcac gccccgccgt gatcggcggc ggcgtgtttg
     2161 cgctgctgga gacgttgaac gacatcggcg ccgtcgaaca cctcggcgtc caatcgctga
     2221 gcctggcgat caagtcggtc tggctcaatc gcggcaacct gcccggcgcg gcgcaaatct
     2281 ccttgctgtg cctggtgatg gtcgtggcga tcctcatggc ggaacacgcg tttcgccgtc
     2341 atgccgccta cgccggctca tctcgcgcca cggcgccgct gacacccatt cgcctgaccg
     2401 gatttcgcgc ggcactggca agcctcgcct gcgctgcgcc tgtcatcatt gggttcgcgc
     2461 ttccggcggc ctttctggcg gtcgaagcct accggatggt gctgaagtcg ggcatttctc
     2521 ctgcaatcgt gcacgctctg gccaacagcg tcatgctctc ggccggggcg gcggccatcg
     2581 tcgcggcagt gggaatcagc gtggcgctgg ctgcccgcct gctgccagac aaggtgacaa
     2641 ctctcgcgcc gcggatcggc gccattggct atgcggtacc gggcaccgtg ctggtgatcg
     2701 ccctgcttcc ggtcttcgcg gttgtcgaca acggcctcga agtcgcagga ctgtcgctcg
     2761 ctttgtccgg cggacttgcg gccgtactga ccgcctatgt cgttcgcttc accgggatgg
     2821 gaatcgcgca ggccgaagcg gggctttctc gcctgtcacg caacatcgac cacgccgccc
     2881 gcatgcttgg agcgaaggcg aaccggctgg ccttcaccgt gctgatgccg aacatcctgc
     2941 cggcggtcgg tcttggcgcg ctgctggtgt tcgtggatgc gatgaaggaa ctgccggcga
     3001 cgctgcttct gcgccctctg aatacggaaa cgctcgccac cagcctttac gcccatgcct
     3061 ccgccggttc attcgaggat ggcgccatcg aaggccttct gctactgctg atcggcctga
     3121 tcccggtcag catactggcc agggctcggt cggcttgaat ggcgttgcgt ttgcctgccg
     3181 gacactatgc atgtaagacc ttgcatcctt gcgcgaagga gccgcggccc tgccgctcct
     3241 gcctgctgaa taccacctgc attcgcctcc gtgcgcggga gatcacggtt tgaaagacct
     3301 gctgcagcgt cataagggac ttcttggcgg cgcggtcatc acagtgacag tggccgccgt
     3361 gctggcgttc gtgccgtgga cggctcccga aagctggcgc gaaaccttga cgcgaaggct
     3421 ggcgctctcg ctcggcgcac ctgaactgac agcgaccggc gcgcactttt cgctgttgcc
     3481 ctcgccgcgc gtttcgctca cgcgggtctc cttcgcccag aattccggca cggtccgggg
     3541 gagcgcagag gaggccgtcg tcgacctcag cctgctcgac ctgcttgccg gaagggtcaa
     3601 acctgcggcc ctgaccctgc gccgtccgga cctgacgatt caactcccag agcagagcga
     3661 gaagggcttt cgcagcgcca tcgagctcgt ctcgcaggtc ggtggttccc tgctgctagg
     3721 gcaggtttcc gcaccgctgc cgcggctgga tatcgacggc ggtcgcattg cgctttcgcg
     3781 cggcccccat ggacccgctc agaccttcga cgacgcccgc ctgcggctgc gcctgccgaa
     3841 ccttcggtcg ccgcttgcgg tcaccttccg cgccaggcag ggccagacgg aaacccgctt
     3901 cagcttctcc ggagccacgc cggcgcgcac gctcgccaac agccgcgagc ccatcacttt
     3961 cgcttattcc ggccagggca tcaccctcaa gttcgatggc gaaggcattg tttccaatgc
     4021 cccgtccctg aacggaaagc tgtctgcggg aacgaccgcc gccgcgctcg gcgcgctggg
     4081 atttccggcc gtcgggaagc cggcggagcg tgtgacactc aatgcgctgc tcgactattc
     4141 gaaccgcggc gccaatctgg ccgacctcaa gattgcagtc ggcgaagaac tgtacgacgg
     4201 cgtgggctcg gttcggcacg atggccagcg ctggcatgtc agtggcacgt tggctacggc
     4261 caaggccgat atgtccaccc tgctctcgcc gatcacggcg ctgcgcaagc cggatggccg
     4321 ctggaacgac acggcgctcg ataccgacgc cctgttcgcc agttcgatcg atcttcgcct
     4381 gtcggcggac cagatgctgg tgttccagcg aaagctggaa aaggtcgcgc tgtcggtcat
     4441 caccaggcaa agcaaggccg aagtcacgct tggggatgcg agttggcaca agggcgcggt
     4501 caagttgcgt gcctcggccg cgcccggctc gttcggcgtc gatctgaaac tcagcgcttc
     4561 gctggagaag gccgatttcg gcgcgatcag cggcgacttc ctgcgcgaac ggcgactcag
     4621 cggccagggc aacgccaaca tcacgctgga aacgagcggg cgcagcatcg cgcagttcat
     4681 caccaacgcg gagggacgct ttgcagcgat tctgcgcaac ggcgaattgc atggcatcga
     4741 tctgaaccga ctggcgagcc ggcgcgcctc gcgaggcgac ctggcgctga ccgaagctct
     4801 cggcgggcgg acctcgttcg aaaccgcgaa cctttccgca aagatcagca atggcgccgc
     4861 ccagcccgtg gatggccata tgcgcgcggg gcggctggtc gggaccctgc aaggcagtgt
     4921 cgacttcgga caggccgagc acgacctgtc cggcctcgtc gtgcaaacgc cggctgacgc
     4981 tcaggccgcc gagcccgcct cggtgctcga attctcgatc cagggaccgt tgctggaacc
     5041 gcgtatcacg ccgaacgtcg cggcactgct gcgcaggagc tgatgccccg gcccgtcatc
     5101 gcgaacggat catgccggag cgacggtgtt tcgcccgccg aatttcgcca gaagcgagcc
     5161 ggcgaggaca atgatcacca caacgaggat catcagcgtc gaggcggcgt tgatctcggg
     5221 cgtcaccccc aggcgcacct ggctgtagat ccgcatcggc agcgtggtcg agcctggacc
     5281 ggaggtaaag ctggcgatca ccaggtcgtc cagcgacagg gtgaaggcca gtacccaccc
     5341 cgccccgacc gccggcgcga tcaggggcaa ggtcaccagc aggaaggtct gaagcggcgg
     5401 cgcgccaaga tccatcgccg cttcctcaag gctgcgatcg aaccctgcca ggcgtgactg
     5461 aacaatcacc gcgacaaagc cgatggtgaa ggtggtatgg gcgatggtca cggtccagaa
     5521 gccgcggtcc atgccgatcg ccacgaaaaa caacagcagc gacaggcctg tgatgatctc
     5581 cggcattacg agcggcgcat agaccatgcc ggcgaatacg gcgcgaccgc gaaaccgccc
     5641 gctgcgcgca agcgcgagcg ccgccagcgt gcccagcacc gtggcgatcg ttgccgaagc
     5701 gatgccaacc ttgagcgaca tccaggccga atccagcagg gcctgattgc gcagcaggct
     5761 cccgtaccat tgcgtcgaga aaccggccca gaccgtcacg agccgcgaag cgttgaacga
     5821 gtacagaacg agcaggagga tcggcacata caagaaggca agcgccagcg tcagccttac
     5881 caggtcgaaa cgcgacagcc ggccactcat cgcgtcgtct ccgcctgccg ctgttgccag
     5941 atcaccggga gcagcaccac ggcaagcagg cagatcgcca tcgctgccgc aagcggccaa
     6001 tcccgattgg cgaagaactc gttccagagc gcccgcccga tcatgagcgt ctcggaggag
     6061 ccaagcaggt ccgggatgat gaattcgccg accgcgggaa tgaacaccag cgcgcagccg
     6121 gccaggactc cgggccagat gcaggggact gtaaccagga agaagctctt caatggcgtc
     6181 gcgccgagat cggcggccgc ctcaagcagg cttgcgtcca tcttctcaag gctggtgtag
     6241 agcggaaaga ccatgaacgg cagataggaa tagacaatgc cgatcaggat tgccgtttcc
     6301 gtgttcagaa cgatcagcgg ctcggagatc aggccaagtg acatcagaag gccattgaga
     6361 agcccctccg gtttcagaat cgcgatccag gcataggcgc ggatcaggaa actcgtcagg
     6421 aacggcagca ccagcagcat gatgagcagc ggtcgcaaac ggcgcggcgc gcgcgccatg
     6481 ggcagggcga gcatgaggcc gaccagaagc gtcagcagcg tcgccagcgc ggcaatgcgc
     6541 agggacgtca cataggcgcc ccacatcaat tcgtcgtcca gaatggtgct gtagttgaac
     6601 aggttgagct gggagagaaa cgtcccgatc ccctcccacc cggccgagag gtccaggatc
     6661 ggtcggtagg gcggctgcgc cgtgaccttt tccgacaggc tgatgcgcaa cacgatcaga
     6721 aacggcaggg cgaagaacgc gcccagccaa agcgccggcg gcaaggccgc gacatggcgg
     6781 aacaggcgac gcagagacat cgcgctcagc cctgcaagac aatgccggca tcggccggga
     6841 cggtaacgac aaccctcgtg ccgtgctgca acgcgctgcc ggcagcacct cgggtattcg
     6901 acacggtgac gcgcaggacc gcgccaccat ccagcgcgac gcggtaggcg gtctggtccc
     6961 ccaggaacgc cgtgccggca atcgtaccga ccaatcgcga ccgcgttccg gtcgctccgt
     7021 ccggcgcttc tgcagacagc tcgagacgct ccggccggat gcacagcgca gtcgtcttgc
     7081 caatctgaag ttgcgattca agaacttcac gttctgattc agaatccgga accgccagca
     7141 agatgcgctc gccaccgggc tttcgccgca gcacggccat atccttggaa acggattcaa
     7201 caatgcattc caagatattg atatcgccga taaaattggc gacgtaccga ttcctcggcc
     7261 gctcgtagat cgcagccggc gcgccgacct gcgccagctt tccgcgctcc atgacgccga
     7321 tgcggtcagc cagcgccatg gcctcttcct ggtcgtgcgt gacgacgatg aaagccaggc
     7381 cgagcgttct ctggatctcc ttgagttcga actgggtctc ctcccgcagc ttcttgtcca
     7441 gcgccgccag gggttcatcg agcaacagca gccggggccg cttcgccaga gcgcgggcga
     7501 gcgccacgcg ctgccgctgc ccgccggaca actgatgcgg cttgcggctg cccaatcctt
     7561 caagtcggac cagacgcagc aggccagcca cccgctcggc gatgcgttcg cgcgccatcc
     7621 cctcccgctt gaggccgaag gcaatgtttc cctcgacgct gagatgcggg aacagcgcat
     7681 aggactggaa catcatgttc accggccggc ggtaggctgg cacgtcggaa atgttggcgc
     7741 cgtcgagcag gatcgcgccc tcgtccgggc gctcgaaccc ggccagcatg cgcaggagag
     7801 tggtcttgcc gcagcccgac ggcccgagca gcgcgaaaaa ctcgccctgc cctacatcga
     7861 gtgtcacgcc gtcaaccgcc gtgaacgcgc cgaagcgctt caccaccttg tcaatacgca
     7921 ccaacggcgc agcgcccgat gcattcccgc caacggactg gggcaaagcg gccattccat
     7981 ccctcgaagt gctgtggaca gccctttcat gggacaaggc ggggagcgga gcaagacttc
     8041 gttgcggtca atgccgcgcc ccgcgcttca gccggagcgt tcatagcttg aggcgtgaca
     8101 gcccccgacg gcggagcgaa gcgtcccagc ggcattcatt ccgagccggg cgccagcgag
     8161 gacaacccgt ccgccaacct gaagtgcaag caccgccaga attgctacaa tgtcgagaag
     8221 ccggaacagg tggtgcgcgt ctatcaggac atcgcccaga acttctatac cgcgacgctg
     8281 aagaaatagg ctgcggcgat ccgggctgac attgcaggag gccgggcgtt accctgcctc
     8341 tttcaaggcg ctctggattc gttcgcgcag cacggtcggg tttttcagga cgaggccacc
     8401 gcgcgtcttc tccgccagcc cgtcacctac catgttggtc agttcgcggg tgatctgctc
     8461 gcgccggcag ccaacccggc ctgccagcac atggtggaac ggcggcggtg aaacgatgcg
     8521 ctcgatcgga ttccccgagc gcggctgtga ctggcgcaac agttcggaat agagccgatg
     8581 gcgcacgtcg agcaccgtgt tctcaataag gcggccgttg aggtcgcgca cgcgctgggt
     8641 aagcagccgc agcaactttt ccgccaggac cttcgaggaa aagatgatct cgcggaacac
     8701 ggatccggaa atgatgcaca tctcgcaccg cgtgagcgcg gtgacgttgg ccgaacgcgg
     8761 cgtgccgtcg atcgcggaca actcgccgaa gtagctgccg gcatgcatgt ccgtgagaat
     8821 gacttccttg cccccctgcg ttcgcaccag gatgcggaca tcgcccgaga tcaggaagta
     8881 gacgtcgctc gacttgtcct cgaaatcgac gacaacctgc tcttcctcca gcttgcgcca
     8941 ggtgcatcgc cggtcgaaat tcttgaggtc gatgtcggtc gcatccttga agaatggtat
     9001 cgacgcaagg gtctgcatgg ctatggcgct ccctccgtcc ctgccccgcc gcaggagact
     9061 cgcgctattg tgcctgagga attgccggta gcaacaggca cgaaccgctt gcgccacgtt
     9121 tttcgggatg gacggtccgg ttccctttcc taatatcatg cggaacactc aggagtcgct
     9181 atgccccttt attcgctcga cggcatttcg cccatcgttc cggcccccgg cgactactgg
     9241 atcgcgcccg atgcccacgt catcggcagg gtgcggctgg cgcgcgacgt cggcatctgg
     9301 tttggcgcgg tcctgcgggg ggacaacgaa gagatcacca tcggcgaagg atcaaacgtg
     9361 caggagcaca gcatgctcca taccgacatg ggtttcccgc tgacggtcgg acgcggctgc
     9421 accatcggcc accgcgccat ccttcatggc tgcaccattg gcgacgggtc gctggtcggc
     9481 atgggggcga ccgtgctgaa cggggtcagg atcggccgca attgcctggt cggcgccaat
     9541 gcgctggtga ccgagggcaa ggaattcccc gacaactcgc tcatcgtcgg cgccccggcc
     9601 aaggccatcc gcacgcttga tgaccgtgcc gtcacggcca tcgcggccgg ttccgccaat
     9661 tacgtccaga actggaagcg ctttgcctcc agcctgaagc cgatcagcta gttgcctgcg
     9721 ctatttcggt gccgtcgcca ccattgatgg acgggtcgag aagctctcgt accaggccgc
     9781 gacttttggg tgatccttgc gccagtcgta gtccggaaag cggaaatcga gatagccgca
     9841 caggcagccg gccgcgatga cgccaatgtc gacgtgaccg gcaagatagc ccgtccagtt
     9901 ctgttcaagc gtgtcgaagg acgaggtgat cttgtccatc tgacccgcgc gccactccgg
     9961 ccagcgcaga tgctcgggcc gcaagaccgt ttcgtagcgc gccagcagcg cggcatcgag
    10021 cgcctcgtcc gcgagcgact gcagcaccag cgccttccag cgcccgcgac cggcgggaaa
    10081 catcttcgcg cccgatgcga ttgaatcgag atactcgcag atgacacggc tgtcgtagac
    10141 cgcttcgccc tcctccgtca ccagagtcgg gatcttgccg gacggattga cgtcgacgat
    10201 ggacttgtct cggctgaccg gactgaccgc cgccggaact acggtcaggc ggtcgccaag
    10261 cccgttttcc agcgcctgcg cccagacctt gcgggcaaac ggcgaggcgg cggaatagaa
    10321 aagcttcatg gggcgtgtct cctgcatgct cgggtacgga ccggcaagac tagccgccga
    10381 tctgcccgcg tgggaagatg aaaaacggcg ccgatgcgtt cccatccacc gccgaacgag
    10441 ccttgcatga gtggcgcggg tcaggcatag agcgccgggg cgcgagcctg gagacgtggc
    10501 cgagaggctg aaggcactcg tttgctaaat gagcataccc tgaaaggggt atcatgggtt
    10561 cgaatcccat cgtctccgcc attacctgca gatggctcgc cttccggagc gcggcgcggc
    10621 cgtcaaagcc cccggcaatc atggctgacg catcgtcggt cgttggaaaa acaatcgttc
    10681 tgggaattct caccaaccat tccgacggcg ggaacgggcg atgatccagt ttcggctgat
    10741 gcgatcacta cagaaattcg ccgcagtcca cggctcgatg cacgaccact tcagcctaga
    10801 acccacaccc atcagtaggc ggaatttcaa ggcccgtcgc aacgccgcgt tgcagccgcg
    10861 gcgcgcccag gacgctgtcg gaaagaacct cgcgctgcgc cgaaatccaa gactttttcg
    10921 gttagtccga caccactcac ggcctcaggc cgggcgcctc gtgcccggta cgggcaacat
    10981 actcggtgta gccgcccgga tagatgtgga cgccgtcctg cgtcagttca agcacgcggt
    11041 tcgacatcgc agccagaaag tggcggtcat gcgagacgaa cagcatcgtc ccctcgaatt
    11101 gcgacagggc ggcgatcagc gtttccttgg tggcaatgtc gagatggttg gtcggctcgt
    11161 ccaggacgag caagttgggt ggatcgtata gcatatgcgc cattgcgagc cgcgccttct
    11221 cgccaccgga cagcacgcgg cagcgctttt cgacatcgtc gccggagaag ccgaagcaac
    11281 cggcgagggt tcttagcgac ccctgcccgg cctgcggaaa ggcccgctcc agcgattcga
    11341 acactgtttc ctcgccatcg aaagtttcca tcgcgtgttg ggcgaagtag cccatcttca
    11401 cgctggcgcc gatggcaacc gtgccggaat caggcgtgct cgccccggcc acgagcttga
    11461 gcagggtgga cttgcccgcc ccgttcacgc ccatgacgca ccagcgctcc ttgcgccgga
    11521 catggaaatc caacccctca tagatgacgc gggcgccata gcgcttgtac acgcccttta
    11581 tgctaatcac atcatcgccg gacctcggcg cgggctggaa atcgaacgcg accgtctggc
    11641 gccgcttcgg cgcctccacg cgctcgatct tgtcgagctt cttgacccgg ctctgcacct
    11701 gcgccgcatg agacgcccgg gccttgaagc gttcaataaa ggcgacttcc ttagccagca
    11761 tcgcctgctg gcgctcgaac tgcgcctgct gctgcttttc gccgagcagg cgttgctgtt
    11821 cgtagaaggc gtagtcgccg gaatatgagg tcaatgtccc gccatcaatc tcaacgatct
    11881 tgttgacgat gcggttcatg aactcgcgat catgcgaggt catcatcagc gcgccgtcaa
    11941 acccctggag aaaggtctcc agccagatca ggctttccag atcgagatga ttgctcggct
    12001 catccagaag catcacatca gggcgcatga gcagaatgcg cgccagttcc acgcgcattt
    12061 tccacccacc cgacagggta ccaacgtcgg cgtccatcat ctccgggctg aatccaagtc
    12121 cattcagcac ttcccgtgcc cggccgtcga gcgcgtagcc gtcaagttcc tcgaatcggg
    12181 cctgcacttc gccatagcgg acgatgatgt cttccatccg atctgcctgt gccggatcgg
    12241 ccatcgcgga ctcaagcgcc ttcaactcgg cgatgacgct gcttaccggc ccggcgccgt
    12301 tcatcacggc gttcaccgca ctgtgcccgg ccatctctcc gacatcctgg ctgaaatagc
    12361 cgatggtgac accgcgatcg accgagacat tgccctcgtc cggagcttcc tcacccgtga
    12421 tcatgcggaa caacgtggtc ttgccggcgc cattcggacc gaccagcccg accttctcac
    12481 ccctgttgag ggccgccgag gcctcaatga acagcatgcg cgtgctggtc tgcttgctga
    12541 tggactcgag acggatcatg cgcggacaaa ttccgaggtg caggaagggg taggcaggcg
    12601 ccaggcccgg gccgacctag cgcccagata cctgcgccgc caggaccgcg caatcggcga
    12661 tctccggatc gacccgaagc accaaagcta atcctggcgc gtcttcgagg ccccggcact
    12721 tcagctttcc acggcgccga ctcaaacgtg cagcgtcagt actcgatttc cgcggcgatg
    12781 ccgacggagg tcttgccgct ggcaccggtt tcgccctgaa gctttatgcg tcgcccgaca
    12841 tccacattcg cggttacgcc gctctgctcg ggcgtcgcgc ctgccttcac gccaaggcgg
    12901 atgttgcggg tgatgtagcg cgacgcgcca actgtcggtc cgtccttgcc ggccgtgaca
    12961 tccaacgtgt cgacgccgag agcctgccgc gttttctcca gaaagtccgg gccaccgcgg
    13021 ccggtcaggc gagccaccgc ctgcgccagt tgcaccgcct gcagcggcga caacgatccc
    13081 actgcctgct cgaacatcag ccgcgacagg acctcgtccg gcggcagttg cggttccgaa
    13141 gtcagcgcga aggacggcga gtccgccctc cccgtcaccg aaaccttcgc ggtgatgctg
    13201 ccggccaagg tggcggcggt aaagtccagg tccgggatca agtctccgag gaaggtcacc
    13261 cggccgcgcg agaattccag gcgttgcgtc agcatgctga gctgtccgcg ccgcagttcg
    13321 aacgcgccgt tggcgcgcgg cgccaggagc ggcccggaca gcgcgatatc cccgccaagt
    13381 tccgcgtcaa tgccgcgtcc acgcacaaag atgtggctcg gcgcaacgat ccgaaggttg
    13441 aggcgagcgt tgccggcggc gcctgccttg cgccgcgcct tgcgctggcc ctcacgttcg
    13501 gcgagacgga gccgctctcg cgtctgttcg ggcggcgata gatgtttggc attggcgagc
    13561 ggcgcggaat caacgggcag ccggtccgga accgtgacat cgaacgacac gacattgacc
    13621 tgaccggcga cgacaggcgc gctcgccaac ggaccggtga ccgcgacatc gagaccggcc
    13681 accagccgca cgacctcatt gtgcagcagg cgcgcattct ctccggtgac gcgcaggttt
    13741 gcaggaaatc cacgcgctgg atcgacgtgc acggttccgt tgacggctat ccgtccctcg
    13801 cccggggtga ccgccgtcac gttctcgacc cgaaggctgg ttcccgcgcc cgctatgcgc
    13861 cctgacacgt tggtcaggcg aaagccctgc aacagatcgg aaaacgagcc gttgctgaga
    13921 acggcggcgc cctcgaaccg aggctcggcc agcgtgccgc gcacggaggc atccagtgca
    13981 actctcccgg ccacccgctg cgcgccaccc gtcaaccggg aatttgcaag ggcggcatcg
    14041 actgtccccg taaccttgag accaagcccg cctcgcgtcg aaaacggagc atcgccgctg
    14101 accttcaggg cgatttgccc acccccccga accgatgcat cgaggcgcac tctgtcgccc
    14161 atcagcgcgc cggtggtagc gatgtcgagc cttggcactc cggcgcgctg caagtccgga
    14221 tattgtagcc ccgccagcgt cgctcgcagg gttccagcgg gccgggcgag atcgccgtgc
    14281 agcatcgcct cgccatcgag gctggcgagt gccgcgccaa agccggaaag gccgcgccct
    14341 tttgccttga tcgccgcctg ctgcgcaccc tgcggcgccg tcaacgtcgc ctgcgccgac
    14401 agcacgccac gcaagcgcgt gcccagcgcc gccgagaact gcccgaggtc agggaaatcg
    14461 acgctcactg ctccggtcac caggccgcca actgctacct tcaaggcgcc ggcgactttc
    14521 ccgccagcca ccctgagatc aagggaggca atatcaagcg cacccccacg ctcggcaacg
    14581 aacgacgtct gtccggacag cgcgcggcca tcgagcttgc ccgccagcgt gatcgctcca
    14641 cgtgtcgcgg aggccgccgt ttcaaccgac ccactgagca cggcatcaac ccgccgtgcc
    14701 ttcagtgcca gattctcaat cgcgacgcgg tccccacgca ggcttgcctt gccgctcagc
    14761 gccgcctgac tgcccaatag cgcggtgagc tgtgcgttgc cgaaagtcag cgtgcgcccg
    14821 atcacggcga gggacgcgtc cagtgtcctg tccagcgggc ggcccttcac cgccgccgag
    14881 attttcgctc caccgcgaat ggagaggccg agatcgtcgg cgaacgcctc cagtcggctg
    14941 atctccgcct cgatccggcc atcgatatcc ggcgcatcca aagtcccgcg cgcccggaca
    15001 tcgagcatca ggccggacaa agcggcgaga cggggggtgc tcgtcctgtc gcctggcagc
    15061 gcccgcgcat tggcctggac gtccagcctg cgatcggacc cgagcgtacc tgcaaccgtc
    15121 agttcggctt gcgtggaggc aagccgcagt ccgggaaggg caatcgcgcc atcgtcggcc
    15181 agatcgagct gacctgttat gctggtctcg ccggaaaaga tcgtcgcggc cacgggcggc
    15241 aacaggccgt gcacctgccc ggcgatattc agctcaaccc gccgcgacgc tccttgccgc
    15301 gtgatggtgg cgcgacctgt ggctcggcct gcatccccgg cagaaaaggc cagttgtgcg
    15361 cgccatgcgt cgagcggccc cctgccctga agatcaagac tgaccgccgg gcggtccttg
    15421 atgtcgagaa cgtgcgcgac cagcccgcca gggctttcct ggtgttgcag gacaagatca
    15481 agcgtctcgc ccttcggaag gaactgcaat cgaagatccg acgtgccatc cctgtcgcga
    15541 cgtcgaagcg cgatggccgc ctgcaaccct tctgagggag caccgaggct gagattgccg
    15601 gtaaccgcaa gtcttgccgg tgatccggca atgggggcgc cgagcaccaa ttcgccaacg
    15661 gaaaacgcgg ccaccaccac cttcaccggc aattccggca ataacggttc gtcgtttccc
    15721 gctgcttgcc gaaccgccgg caccggcttg cgcaggacct cgatacgtcc gatctcaagc
    15781 tgctccacct caagccgccg cgacagcagc gccgcgcgtc gccagaccag acgggcgctg
    15841 tcgacccgca gccacggtcc ctcccggtcg gaaatctcga tgtcgcgaat ctgcgcgttc
    15901 gacgaaagcg gtccgtcaac cgcaccgatc cggacctggc cctcgggcga cgacagcgcc
    15961 gtcgagatga ccttggctaa cacggaccgt tgatcgtcct gcgcgcgccc ctccgtcgcc
    16021 cccaccagca tcagcccgac aagaagcgtc gctatggctc ggacaacgcg catcagaagg
    16081 cctgcccaag gctgatgtaa agcgcgacag gcttttcccc tcgccggcgg tccaacggcg
    16141 tcgccacatc gacgcggatc ggaccgatgg ccgtgtgata ccgcaatccg acgcccagcc
    16201 cgaaccgcag ggtttcatca aaacttggcg cggcggagga aaaagccgcg cccacgtcga
    16261 cgaacggcac gatcccgatt gtttccgtgg cacggatgcg cgcctcgagc gaggcctcga
    16321 acaggctgcg tccgcccacc agccgcccca gggtatcgcg cggacctatg gagcggtact
    16381 catagccccg gacggagccg ccgcccccgg cgaaaaaccg ccagttggca ggaatttcgg
    16441 agaccgacgc gccggatgcc gcgccaaggc cgacacgccc cgccagaata acgcgggcat
    16501 cctgatccag cgcgtagtag gctgaggctt gcgccttgga gacggtgaag aaccgaccga
    16561 tgtcaccggc gccgtaggct gccatcaccg aaccggagag gcgaaagccg cgcgtcgggt
    16621 ccagatcgtt gtcggtcgtg tcccagttgg ccgacagcgg cagaccgaca agctggtaag
    16681 tcagccggcc gaacacatcc tgcgtttgtc cgaattccgc cattgcgccc gcctgcaccc
    16741 aggtcttgtc cgcgaaacgg taacgcagcg cggcagtcac gttggcgatg cggctgacat
    16801 aggcttcggt cgcggcgcgt gacagggcgg cgtcggcaag gaagtcaacg cgcgagccgc
    16861 caagcgccgg cttcaggaaa ccggcttcca gtcgcccgcc gaggttgttc gtttcaaacc
    16921 ggttgcgcca cgcccgcgag tacgtgttcg gcaagtagaa aaggtcagcg cccagtcgca
    16981 ggcgctcagc gccgccaaac atgttgcgat ctgcccaata caggccgatg ccagggccat
    17041 ccacattgga atagcgtgcg gaaccgccca agacatgacg cggtcgcacg ccgacctgca
    17101 catcgaccgg caccgtgcca tccccatcga gcttgtcgcg cggctgaacc cggacggagc
    17161 caaccgcctc gatccgcgcg acggagcggc gaatttcggt catccgttca ggcgaaaaca
    17221 gcatttcgcc ttcggcataa atgaacgagc ggatgacgcc cgcatcgata ccgtcggcgc
    17281 cgctgacatg gaccgcgccg atgttggccc gcgctcctgg ccgcaccacc agcacaacat
    17341 ccacgctgcg gctggcgtgg tcgatcaccg gctcagggtc ctcgatcgaa gcaaagggat
    17401 gaccgagctt ccggtaatgc gcgccaatac gcgtcgccgc ttcgacgatg ccggcggagg
    17461 ccgccggagc gcctgcaaga tgatgataga gtttggtcgg cacatctcca gcgtcgcggc
    17521 ccttgtccgt tcgcacgacc acggaacgga aggcatagcg gggaccggag gcgacaatca
    17581 ccctgacagg aacgatccgg ctgcctcggg cgcgctcggc ggcgcgggcc gcggcaagct
    17641 ggcgttcgcc atgcaggaag agccgctcgc ctgccacctc gaccgtaacc ttcgccgaat
    17701 aatagccttc cccccacaga aggtcggtga gtttcggcag atccgcctcg gcgcgacgca
    17761 ccaggtccag accgtcgaca ggcggctcct gctgcagccg cttgagcacg gatacatccc
    17821 caagtgccgt tttcagatcg ccgtcatctc caacaccgat gatatccagg tcgtaaggca
    17881 aggcgttctg ggctggtgcc agcttcggac ccccactgaa taggctggaa aatccgaaag
    17941 cgctggccgg aagaaacgca cagaatccac aaaaaagcgc caaaagccct acatttcgca
    18001 ctactcttgc agtggcggac atgcggcttt gcctgctcca gttgtcgcgg aaagaccatc
    18061 ggccaaaggc tctctcgccg cacctttggc atgacgctgg cgcgagcgcc ttccaaactt
    18121 taagaaagtg ttaacgggta agcgcaaccg gagcaaggaa cgctcttgac gagcgccccc
    18181 gaatttccac cgtcggtacg atggggccat cgccggcgag atggaccaag cggctcggcc
    18241 catgcgggat tggccttttt tctttggtcc gatggtgcag cgttcagcga ggaccccacg
    18301 ggtttcttgg cgatggagcg gttgcctcgg tagagaaagt ttgaatccag gcactttccc
    18361 caagttatca actgcctcaa gaaggcaatg tcagcaggcc tgcttcacaa actgctcaag
    18421 agaggcggtt ttttgggccg gcttcgcttg tagcgggtga atccgtcggc tttcccgcgg
    18481 cgattttcgg ccgtctaacg ggcggggcga cactcaggtg aatttcagct tcagccgccg
    18541 ttgcagccgg gtctggaaat agaccgcttc atccaccgtg ccggcctcat aggcccaccc
    18601 gtcatcatcg ggcagaacgg agacgtttcg aacgctggga tgcctggcaa tctcctctgg
    18661 cacgatgtcg acaccgaggc ccggggttgt cggcaaccgg aggcagccgt cttccagaac
    18721 cggtggcgcc gagatgacgc tgttgcgccc cggccagtcg aattccattc tttccagcat
    18781 cagcgcgttg gggatggtgg ccatcacgtg cagcgcggcg aattccgcca ccgggccaag
    18841 cgaacccgaa tgcggcgcca tggtgatgta gtgcgcttcc gccatcgccg cgatcttgcg
    18901 catctgggtg atgccaccgg cgcgtccggt atcgggctgc accacgtcga ccagttcgcg
    18961 ttcaatcaga ggtcgcaggc cccagatggt cgacagccgt tcgccggcgg cgagcggcac
    19021 gccgacgttg tcgcgcacac gctggaacgc atcgatgttc tccggcgcga ttgggtcctc
    19081 gtagaacagc acatcgagag gttcgagcgc gcggccgatc tgaatggcgt cctgcggcgt
    19141 cgcccagctt ggcccatgcg catcgaccat cacgtcgatt tccggcccca ccgcatcgcg
    19201 gacggcggcg accttggaca catccaccgc gccggtgaac gacaccttca tcgccttgta
    19261 gccggcgctg agcagggctt tcgcctgcgc cggggtcttg gcgtgaccgt agaccggaat
    19321 tctatcacga aacttgccgc cgagcaggtt ccagaccggc tggcccagca ccttgccctt
    19381 gatgtcccag agcgccatct cgatgccggt gagcgcgccg ccaccgaccg tgccgagcac
    19441 gccgtggccc attgagccga tgaagagctt gttccatagc cggtcgatgt cagcgggatc
    19501 ctcgccgaac agcaagttgg cgtagtcctt caccgccgtc tcgatgacgc gcggccagcc
    19561 cgaacattcg ccgacgccgt agatgccttc atcggtcgtg accttgacga acaaccagtt
    19621 cctggaaccg cgaaagtcgg cgtagatgcc cttcgtctcg ttcgcggccg atggacgggc
    19681 gccgacctgc atcaggtagg tttcaacacc ggtgatcttc ataggttgtc tcctgcgttg
    19741 tctcttgcct accctggccg cgcctcccgc ccagacgggg cggcaaactg gggcgtgtgc
    19801 gcgacggccc acgcaactct aggccggctc ccggtatcgt gtgcgcaccc gcgtgccgcg
    19861 catgcgcggg tctgggtgcg gcactgccga cagcagcgcc tgcgtatagg cgtgcttggg
    19921 ggtgttgcag acctcctcgg tcggtccttc ctcgacgatc cgcccgcgat acatcacggc
    19981 aatacggtcg cagaaatagc ggatcaccgc gatgtcgtgg ctgatgaata tgaaagacag
    20041 gtccagccgc tcctgcagat cgagcaacag gtcaagcacc tgcgtgcgga tcgacacgtc
    20101 gagtgcagag gttgcctcgt cggcgatgat gatgcgcgga tcaagcgcga tggcgcgcgc
    20161 gatgccgatg cgttggcgct gcccgccaga gaaggcatgc ggatagcgct ccatcgcact
    20221 ggctggcagg ccgaccatct gcagcagctc agcgacgcga tcgttgagcg ccctgccctg
    20281 caacatgccg ttgacgagca tcggctcgcc gatgagttgg gcaaccgtca tgcgggggtt
    20341 gagcgaggac atggggtcct gaaacaccat gcgcagttcg cgtcgcgccg atttcaactg
    20401 gttttcgtcc agcgtaacga gatcgcgggt cacgccgtcc ttgccggtgt agttcacaga
    20461 gccgccattc gtgtcgtaaa cgcgcaggat cgcgcggccg agcgtcgact tgcccgatcc
    20521 cgattcgccg acaatgccga ggttctctcc ggaccggagc tgcagcgata cgtcgttcac
    20581 cgctaccaac ggggcggtct tcctgaagaa cgatccacga tcgatgtgga attccttgcg
    20641 caacgcgtcg acactgagca gcacatcgcc gggcgggttc gaggcccggc gcgcaagccg
    20701 ccgcgtggac ggccggtcaa gttgcttgac cgaaccgagc agcattttgg tgtattcgtg
    20761 ctgcggttcc tcaaagatcg gcagggtatc gccctgctcc accagcttgc cgtagcgcat
    20821 gaccgcgact tcgtcggcaa cctcggcgac aacgcccatg tcatgggtga tgaacagcac
    20881 ggacatgccg ttgtcatcct gcaacttgcg gatcagggtg aggatttccg cctgcgtggt
    20941 cacatcgagt gcggtggtgg gctcgtcagc aatcagaacg cctggattgc aggccagcgc
    21001 catggcaatc atcgcgcgct ggcgcatgcc tcccgagtac tcaaacgggt atcggtggat
    21061 ggtcttgtcc gcgttcggaa tctcgacctt gcgcaacagt tcgatcgcgc ggttcagcgc
    21121 ctcctttttc gagacattct catgcaggcg gatcgcctcg ccgatctggt cgccaatggt
    21181 gtgtaccggc gagagcgagg acatcggctc ttgaaagatc atggcaatgt cgcggccacg
    21241 gacggcacgg atcgccgatg agcgcttgtt gagcgcggcg agatcgatga cgctctcgcc
    21301 gctctgcctg ccgcgtgccg tgttgtggcg atgcagcagg accttcccgg atttaatgtc
    21361 tcccggcgga tcgacgatct gcaggatcga gcgcgcggtg acgctcttgc ccgagccgga
    21421 ttcgccaacc aggcacagcg tcttgccacg ccgcagcgtg aaagacacat cgtccacggc
    21481 gcggaatgga ccatggcgcg tcgcaaactc tgtgacgaga tttttcacct cgatgacggg
    21541 gggcgcggcg ctcatcaacg cccctcctgc gcataggggt cggcggcgtc gcgcaggcca
    21601 tcgccaagga aattgagcgc aaggaccgcg atgcacaccg cgacgccggg cgagaacagc
    21661 caaggcgcct cggctatggt gcggacgttc tgcgcctcct tgagcagcac gccccatgaa
    21721 atcgtcggcg gcagcagacc gaggccaagg aacgacagcg aggtttcggc aatgatcatc
    21781 atcggaattg ccagggagac cgaggctatg atgtgggaga ccatcgaagg cagcatgtgg
    21841 cggaagatca cgcgcatctg cgaacagccg tccagcctcg cggcggtgac gaaatcttcg
    21901 gtgcgcaggg cgagaaaccg tccgcgcacc acgcgcgcaa gttccgtcca gccgatcagc
    21961 gacaggatga cggtgatggc gaagtaacgc caggtcggcg gccagtcctg cggcaatgcg
    22021 gccgaaagcc cgagccatat cgggatggta ggcatcgcca gcttgaactc aacgatgcgc
    22081 tgcacggatg catcaatcca gccgccgaag tagccggaaa ttccgccaag aaacacgcca
    22141 aggaccaggc tcatcagcac gccgaccaga ccgatcgaca tggatatgcg tgttccgtag
    22201 atgatgcggc tcagcatatc gcgcccaagc cggtccgcgc cgatgagata gaacctctcg
    22261 tcagactttt tcggcgcgat caggcgccgt tcgacctcga acgtccccca cattcgcacc
    22321 ggggcgccct tgccgaaaaa ttcaatgtcg acagtcttgg acggatcgga ggtaaaagtg
    22381 gtcgccagcg tgatgggatc gcgcttctgc gccatggcgt agacgtgggg ctggaatgtc
    22441 cagccgccgt ccggcgtcgt cttgatgaaa cgcacaatct gcggcgggtg gttgacggcg
    22501 cgcgcactcg aggcatcggg gtcgaagggc gccagcagtt cggcaaaggc ggcgatgaaa
    22561 tacaacagcc ccacgacgat cagcccgaac accgccagcc ggtgacggcg aaaggcgatc
    22621 cacatcagtt tccattgact ggcctgatcg gtcgaagcgt gctgacgagt atcgatgacc
    22681 atgctcatgc gatcaacggt aacggatgcg cggatcgata agggcgagca agatgtcgct
    22741 caccagcatg ccgacgacag acaggcagca cagaagcagg atgaaggcgc cggcgagata
    22801 catgtcctgc gccagcagcg cctgcagcag cagcggcccg gctgtcggca acgacagaac
    22861 gtaggaaatg atgaccgagc cggagaccag ttgcgggaac gcccagccga tggtggaaac
    22921 aaacgggttg agcgcaaggc gcaccggata cttgaccagc aacttccact cggaaaggcc
    22981 cttggcgcgc gcggtcgtca cgtagggctt gtgcagttca tcgaggagat tggcgcgcat
    23041 gatacggatc aggctcgcgg tcgccgaagt gccaagaatg atcaccggca cccagatgtg
    23101 cttgcacaga tcgacgaact tgtcccagga ccacggcgcc tgctggtatt gttcggagaa
    23161 gagcccggat gcgtcatggc tgaaccaaac gaccgagacg tacatcagca ccaggccgag
    23221 caggaaattg gggatcgaca gcccaatgaa accgatcgcc gagataacat aatcgccgat
    23281 cgagtacttc cttaccgccg aatatatgcc gatcggaaac gccaccgccc aggtgaacag
    23341 gagcgttgcg gcagccatcg ccaccgatag ccccatgcgc tcccagatga ggtccgagac
    23401 gggctgcgac cattcgaagg actggccgaa atcgccgcgc agaacgccgg aaatccagcg
    23461 ccagtactgc acgagaaagg attcattgag tccgtagcgc tcgcgtaagg cgtcgagtcg
    23521 ggcctgatcg acgacatcgc ccgaagccgc aagggtcgcc gccagcgacg tgacgaaatc
    23581 gcccggcggc gcctgaatca gcgcgaaggc caccagcgac accgcaaaca ggaacgggat
    23641 cgaccagaac agccgtttca gcacgtagac gagcaacatg gggccaactc aacgagaagg
    23701 attttgggtg cccgccggga ggcgggcacc cagggcgctc agttggcgta atagtactgc
    23761 tgaagcagcg agggcgcggg cgtcggccaa taccaggagc tgaccatggc atcgggcgcg
    23821 ttgcggatcg tcttcttgcg gattccaaag gtcggcgcgc tggtggcgac acccatcacc
    23881 tcgaactgtt ccgccgcaat gtcggccacc ttgtcgatca gtgcctttcg cttggccgga
    23941 tcggcggtgc cgcgcgcctc gtcgtacagc ttcatgcgct ccttgaccga ggccggcggt
    24001 tcctcgccgg acttgccacc cgtcttgtac cagttcaccc acgggatgcc ccagcgcgag
    24061 tcgtggtgca ccggcacgat ctgctgcgga atgtccccag gcacccaaga cccctgacct
    24121 ccccagaccg cggcgtcatg gtcattggat tcggaggtgc gctgatagaa gaaggtgcgt
    24181 tcaaccgcgt tgacgtccat gtcgacgccg atcttggccc attgccgctt tatgacctga
    24241 agcatgtcga cttgctcggg ctgcagcgtc gggatcacgt cgatcttgaa tttcagcggc
    24301 ttgcccttcc acatccgcat gccgtccggt cctttctggt tcaggccgac ggaatcgagc
    24361 agctggttcg ccttggctgc gtcgaaatcg agatgctgcg tggatatctt cctgtggaag
    24421 ttcggatgat cctcccacgg gccctgatgc cagggcttgc cctgcccgag caacgccgtt
    24481 tcgataatct ccttgcggtc catgccaatc gacagggcaa cgcggaagtc cttcttgttg
    24541 aagagctcgc gcagatccgg gttcttgtgc gtcaggttca ggttgatgac catgttggag
    24601 ccgcccggcg cattggcggt ccagaattca tagccgccct tgtcgcgatt ctgcgccagc
    24661 accggtcgat tggccggcga gtcgagatgg cgcagctgga agtcgatctt gccgccgatg
    24721 gcgtcgagta tcaggctttc aacatcctgt gcgatcgggg aaaccagctg atcgatatag
    24781 ggaagctggt ttcccgcagt gtcgacctgc cagaaatagg ggttgcggtc gagcacgacg
    24841 cgggtggcgc cgccgacgta gggatccttg accacccagg gatcgagcgt cggacgggcc
    24901 ggattgcccc agcgggcgga gatttccaga tcgccgcact tggactggaa caggcctttc
    24961 cagtccgcct gcttgttctc cttgatgagg tcgtcgatct tgtcgttgta tttcggcagg
    25021 aattgcgagc aatagtgctt ggcatagagc accgggttct ggcccagcgg cgaagcaagt
    25081 tcggcaagga agtcgccgta gggctcagcg aaactgaacc ttaccgtcgt atcgttgatc
    25141 ttctccaccg taaaaggctt gccgccggcc atgtagcgcg ggccaagcgg cgtcagcgcg
    25201 ccggcaatga cgatgtcgtt catgttgaac agaacatcat cagcggtgaa aggcttgccg
    25261 tccgaccact tcatgccctt gcgcagggtg aacgtgaaca ccttggcgtc cggcgacacc
    25321 tcgaaggatt tggcgacgtt gggaataacc ttggtgtagc cgagatccca acgcaccaac
    25381 ccctgagggc ccgtcatgcg caggatgttg ttgaagtcgg aggagccacg caggccgtag
    25441 cgcagcacac ccccatatcg gccgacgctc ttgagcgggg tcacgacctc cggttcatca
    25501 ccgacgcggt tcgcaacggg cggcaacttg ccatccttga cgagtgcggc cagcgcgggc
    25561 gcctcactct tggctccctg tgatgcggcg gtgccgactg caagcgtcga aacgccggtg
    25621 gccagcgcgg caacgattag tgctcttctg atatgcgcca tgggcgagat acctccctgg
    25681 ttgccattaa gctgcggtga cgttacacac ttgtcagaca actgacaact atgcgaccac
    25741 cccccatcac atgaaactcg caacgcagct ccaggcacga gacgattcag acttcgaggt
    25801 atggtcccag ctcggcatca cgcacctttg ccaggacccg ccgggcaatc cgcatgactg
    25861 gacgctgtcc gacatcgaaa ggttgcgcga caccatcggt cgctacggca tggtgcatga
    25921 catggtgcag ttgccgctgt cgtcgcgtcc gatcgaacag gctcagagcc cggacatcct
    25981 gctcgccgga ccggagcgcg accggcagat cgattccatt tgcgacctta tccgcaatct
    26041 tggccgcgcc ggcattcctg cggcaaagta caacctcaac atcattggca ttccgcgcac
    26101 gccgctggaa gcgggccggg gcggcgcgaa ggccgaggcc ttccgctggg aattgaccga
    26161 tcagaatgcg ccgcccgggc ttgccggcct gctcgatgcg gacaccaatt gggaacgcat
    26221 tgattatttc ctcgaacgcg tcgtgccggt ggcggaggaa agcaaggtcc gcctcgcctg
    26281 ccatccgcat gatccctaca cgcctccggg ctacaagggg gtgacgcgcg tactcggcac
    26341 cgtcgaaggc ctgaaaaaat tcgtgacgat gcgagagagc ccctatcacg ggctcaactt
    26401 ctgtcagggt accgtgggag agatgctgga agaccccgga accgaggtgt tcgacgtcat
    26461 ccgctggttc gggacgcgca acaagatctt caacgtccat ttccgcaaca tcgccggcaa
    26521 gcgcctgtca ttccgcgaga cgtttcccga agaggggaca atggacatgt ggaaaagcat
    26581 gaagctttac cgggagatcg gctatgcgca catgatcatg cccgatcaca tgccgcatgt
    26641 ctccgggccg aacgaggtcg gcgtcgcctt cgctttcggc tatggctaca tccgtgcgct
    26701 gatccaggca gctgaggccg aacatgcggc ctgagatcgt tgtcgccgcg gacgtgcgct
    26761 gtgagacagg cgaaggggtg ttctgggatg ccgaaacgca agcactctgg tttgtcgata
    26821 ttccagtcgg gcggctctac cgtctgaacg atggcacgct gcatcactgg gacatgggcg
    26881 agccgctcgg ctgcctcgcg atcgaccgtg cgggcaatcc cgtcgtcgcg ctgaaacacg
    26941 acatcgtccg actggatctc tcgacaggcg ggcgggtgct gctggcgcgt ccggagaccg
    27001 accgaccgca caaccgtttc aacgatggcg cggtggatcg gcaggggcgt ttctgggccg
    27061 gcacgatgaa gatgggtcaa ccggcggagc gcaccggcgc tttctaccgg atcgacgccg
    27121 accatcgtgt gacccggttc tgggatggca ccttcaccac gaatggcatc gccttctcgc
    27181 cggatgggcg aacgatgtat ctgtcggatt ccaatcccga ggtccggacg atctgggcct
    27241 gcgaatacga cacggatacg ggcacgccgg gcgagcggcg ggttttcttc gatacgcggg
    27301 ctgtcgccgg ccgtcccgat ggcgcgacgg tcgacgcgga tggctgctac tggatggccg
    27361 gcgtcggtgg ctggcaactt ctgcgcatca cgccgaaggg cgcgatcgac cgcatcgtcg
    27421 acatgccctg cgaacggcct tcacgcccct ggttcggcgg gcgcaacctc gacattctct
    27481 acgtcacgtc catcggcgcc ggtctgtcct caggaactga ggcgcgccag ccccatgccg
    27541 gtgcactctt cgcagttcat gggcttggcg tgacggggct gccctatcct cgcttttgcg
    27601 gatgatttcc aggagctcgt gatgaaaact tccccagtga cagtgcgcga tctgcgccgc
    27661 tccgtgcttg ccgttccccc actctgccgc gatgcggacg gcatggtatc gctcgcggaa
    27721 aatcgtcgcc tgacagcgca tctgcgggcg ggcggcgtga ccacgcacat gtggggcggc
    27781 aatgccaaca tctatgcgct gggcgtgtcg gacttcccgg cctttctcga tgtcgcggaa
    27841 gcctgcgcgg tcggcgatga atgggcgctg ccctccattg gcgctgaatt cggcaaggca
    27901 atggatcagg ccgcgatcct ttcaaggcgc gcctttccga ccgcaatgat cctgccaatg
    27961 cgcggcgtcg tcgaccggcg gggagccgcg cgcggcatcg gcatggtggc ggctaaaatg
    28021 ggcaagccgc tgatcgttta ttacaaggac tccggctatc tggacatggc ggatctcaag
    28081 gcgctgttcg ccgatggctc gctctgcgcg ctcaagtacg gcgtgctgac acccgacgcg
    28141 gcggtggatc cggaactcgg caagattctc aaggccgtgc caccggacct ggtcatttcc
    28201 ggaagcggtg agttgcccgt ggtggcccac gccgagacat tcgggctgcg agccttcacc
    28261 tcgggctgcg tctgcatcgc gccgcgtctt tcgatggcta ttctcgccgc catccagtcc
    28321 ggcgactttg cccgcgcccg cgccatccgc gaaaccttcc tgccgttgga acatgcgcgg
    28381 gagcagcact cgccgatcaa agttctgcac gcggcggtgg ggcttgccgg ggtggccgcg
    28441 accgggccgt tgcagccgat gctgtcggaa ctgacggagc ctgcggtcct cgatgcgctt
    28501 ggacgcgagg caaaggcgtt gctggcggcc aatgaaacca tgaaggtggc cgccgaatga
    28561 gccagcacct gtcgcctcga ccacgcccgg cagcacaatg caaagaccaa caaggacacg
    28621 gtgctcggct aggtgaagaa tgatccgatc gccgaggagt cttacgacga tgccattgtc
    28681 cgttctgaag ttgtcgcctc gtcggcgcgc acgccgaagc ataagcacct gacagcagac
    28741 tggccatgat catcaccgat gttcgcgtca cgaccttccg ccacacggtc cataccttca
    28801 aggatgagga tggacacact catcccggtc cgccgcggca ggtgctttcg tccctcctga
    28861 ccatcgccac cgaagacgcc gaagggcatg tgcacgggcc gaccgaactg ctgcgcccgt
    28921 tcatcgtcga gaccttcctg aagaaggttc tgctcgggca aaacgccttg atgcgcgaaa
    28981 agctgtggca cggccttgcg cattggcagc gtggcagtgc gtcgatgctg accgaccgca
    29041 ttcttgccct cgtggatctt gcgctttggg atctggcagg ccgggcgctg ggcgtgccgg
    29101 tctacaagct gatcgggggc tatcgcgaca aggtgccggc ttatgcctca accatgtgcg
    29161 gcgacgagat cgaggggggg ctgaagacac cggacgacta cgcccgcttt gccgactggt
    29221 gcaagaacaa gcgcggctac aaggcgatga agctgcacac ctggatgcca ccagtgacgt
    29281 ttgcgccctc agtcgacatg gacatcaagg cctgcgcggc ggtgcgcgag gcggtgggcc
    29341 cagatttccc cctgatgctc gatgccagcc attggtattc gcgccaggag gccctgcgct
    29401 tgggcagggc ggttcaggat ctgggctaca cctggttcga ggagccgatg gaggaggcgt
    29461 cgatctcctc ctatgccaat atggcgcgcg aactcgacat tccggtgatc gggccggaag
    29521 tggcggacgg caagtacttc acccgcgccg aatggatcag ggccggcgct tgcgacattc
    29581 tcaggaccgg cgttcatgac gttggcgggc tgacgccggc gctgaagtgc gtgcatctcg
    29641 ccgaaagttt caacatggcc tgcgaggtgc acggagtcgg cgccggcaac ctcacgctct
    29701 gtgccgtgca ggccaatggc cgctggtatg aacgcggcct tctgcatccg catttcgact
    29761 acgacgcgac gccggattac ctccacgagc ccgaagacgc gatggatgca gacgggtttg
    29821 tgcacctgtc gcagaagccg gggctcggcg cgcactacaa ctgggacttc atcaccgcga
    29881 acaccgtctc gacgcattga caggtgcgga aagcgaggaa actccatgaa aatcgtccgt
    29941 ctccgctctt tcatgtcgcg cgatgccaac cgcccgcgcg tccttgtcgc catcgacacc
    30001 gatgagggcc tgaccggatg gggcgagtgc tacaatcacg gaccggacag ggcgctcccc
    30061 gacctgctcg actactattt cggacagatc gtcggtcatg atccgacgcg catcgagttc
    30121 ctctatctca agctgttgca gcacaaccgt tttccgccag gtgcacttgg gctcgcggcg
    30181 atgtcggcca tcgaccatgc actgtgggac atttcggcga aggcactcaa tgtgccggtc
    30241 tatcaattgc ttggcggccg ctgccgcgac aaggtgcgcg tctactccgg cgtctacacc
    30301 gcgcctgatc cggaagcggc gaaggaccag ctcggcaagc tcaacgccga ttggggcttt
    30361 accgccttca agctctcgcc ctaccgcatc gatcaccatt ccaaccgctg gggcgaggtg
    30421 gtgcgcacct ccgccgagta ttttcgcgag ttgcgggagg tctgtcccgc ccactacgag
    30481 atcgccttcg atgcgcatgc caagatcttt gaggtgcagc aggcgatcca gcttggcaac
    30541 gcgctggcac cctatgatcc cctgttcttc gaggaaccga tccgtcccga gcacatgccg
    30601 gcatggggcg aattgaagcg cgggcttacg tgcacgcttg cgaccggcga gagtctctat
    30661 aaccgctacg agttcctcaa cctcctgagc cacaagggct gcgatatcgt gcagcccgac
    30721 atctgcgtcg tcggcggcat cagcgaaatg cgcaagatcg ccgcgatcgc cgaggcgcat
    30781 ttcgtgacca tcgcgccaca caacccgatg gggccgctgg ccacggcggt gaatgtgcat
    30841 ttctccgccg cgacaccgaa cttccgcatt ctcgaatacc gcctgccgac acagcgatac
    30901 atgggcaccg acgattccga cgagggctgc tacgtcgctg atccctatct gccgaaggac
    30961 ggctttcttg aactgcggcc ggatcgtccg ggctgggggg tcgagatgga catgaagtat
    31021 ctgtccagcg accgttacat ccattgggag cgcaagttgc cgaccaggcc cgatggctcc
    31081 accgcattca tgtaacggac cgcccgatac taagacccct gtgtggcgga catggccgcc
    31141 gcgtggtgcg cttgtcctta tgagcacgcc gcatcgcttg tctgaccctg cacaaacctg
    31201 gatgacgttc agggcctcag atcacccggc acgcggtcag gacgcgcgca cagctttcag
    31261 gccctcgacg tgctccaccg ggccggcgac gtccggggcg agcgtggcga tttccgcgtc
    31321 aagccactgg atgacctgac gggccagggg cgcgagatca aagtagggca ccataaggcc
    31381 gatggcgaag cggtaggcag acgggttgtt gacgatcacc cgcgccagcg tcctgatgaa
    31441 gaaccgccga tgctccggct ggcggacggt caactggcgg acgaggccga aaaccgccct
    31501 gaggtcaccc cggagacgcg agagttccag cctgggagcc ggcgtcttgc cgccgaggcg
    31561 gattgccatg atctcgacgc gcttgaagta ggcctccgga cggtagatgc gatccagaac
    31621 ctcgcggtaa gctttcagga tctcccggcg cggccgcagc gtccggaagt tgagaccggc
    31681 ggtgcattga tccgacgtcc cgtcgtgcgc ggtggccgcg gcgaagtcga gatcggtgac
    31741 cgggtccagc cgcccttctg tgatgagcct gcgggacaac tgggtattcg gcagggccgt
    31801 gagcagaccg atcatgcaca ccggtatcgc agcctgttcg atacaggcga tcatgtcggc
    31861 ggcaatcgaa gcgggctcgc tgtcaaatcc gatgatgaat ccggcgtgga cctgaatgcc
    31921 agcccgattg atgcgggcga cgctgtcggc aagatcgcgg cgcgtgttct gcttcttgcg
    31981 cgccgcgacg agcgtggccg ggtccggggt ctcgatgccg acgaagacga gattgaaccc
    32041 ggctgccttc atgagcgtga gaaagtcttc gtcgtcggcg agattgatcg aggcttccgt
    32101 aaagaattcg aacgggaaac cgtgcgcctg ctgccaggcg acgagccgcg ccaggaagac
    32161 acgcaccgcc ttcttgttgc cgatcaggtt gtcgtcgaca aagtcgacga tacctcgatg
    32221 gcccagacga tacagtgcct ccagttcggc cagcacctgt tccggattct tggcgcgcgg
    32281 aacgcgaccg aacagttcga tgatgtcgca gaactcacag gtgaacggac acccgcgcga
    32341 gaactgcacg ctgatcgacg aatagcggtc gatcttcaga agatcgtagc gcggcaccgg
    32401 aatgcgcgtc acgtccaccg tgtacttctc cgcgtcgaac cggcccgtgc gctctcccct
    32461 ttcccacgcc gctacaaagt cggcgagcac ggcttccgct tcgcccgtga tgcgaaactc
    32521 tgccccttca aacaccgtcg gactggacgt cacatccggg ccacccacga cgacaggccg
    32581 gccgagtgca tgaacgcggt cgatcagcgc gaggcatgcg gcgcgttgag ggatcatgcc
    32641 gccggtcatg acgacatcgg cggcacgaac atcggcatcg tgcaggtctt cgacgttgca
    32701 gtcgaccagc ttgatccgcc agtgcgccgg caatagcgcc gccaccgtga tcaaccccaa
    32761 cggcggcgaa agccagacct tgccgatgac atcggccgtg gtcttcagac tccagaagga
    32821 cgtgctggtg aacctcggga acaccatcag gacattcacg gtcttggaca tctgtccgga
    32881 ttcctgtgaa gtagagcagg caacacggcc aacgcgggac gcgccgggtc cgccagcaga
    32941 tgacatgtat ggcggaacac tctccggacc gccgtaagat ggatcaatgc caccgccact
    33001 cggacggcct atctttacgc atcgcctgca cgtcgcaacc aagcaccaga cgtcggagtc
    33061 aacggaagtc atgaagcagg ccgtcaacgc tgcactctcc tacgccgagg gctggctgcg
    33121 ccgaccgacc cccattgcgg acccggtcat tctcgacatc gtcctgacca aggcatgcaa
    33181 cctccggtgc agtttctgca tctcgtatgc cagtctcgca cagacgcact ggctggacta
    33241 tggcctttac cagcgtatcg cggccgaact gtttccgacg gcctgggatg tacaattttg
    33301 ctcgggaggc gagcctttcc tgtatccaca gatccgcgag gccttgcagc ttgcccgtca
    33361 ccacggctgc aagaccggtg tgacgacgaa cgccatgctg atcgacgatc gcgtcgcgac
    33421 ctggctcgtg gaagaccaga cgcttgaccg catgtgggta tcgttcgacg gcggcacgaa
    33481 ggagacgctg gaaaggctcc gcctcggcgc gcgcttcgac aagatcattg ccaacgtccg
    33541 caacctcgtg gccaagcgca gggcggccgg cgcacgcttt cccatggtcg acatgcgctt
    33601 tgtcatgatg cgctcgaacg tcgccgaact cgcacgcctc atagaactgg ccgcggacct
    33661 cggcgtgcgc cgcgtggaag ggcgatacct caacgtcgcc aatgacatgg accgagacga
    33721 gtccctgttc catcaccggg gcctggcggc cgaggagttc gcccgagcgc gcgcggtagc
    33781 agaagcccaa ggtgtgcgcc tctctctgcc accgttgccc ggagaggacc ggggtcggca
    33841 tcgttgcatc aagccctggg agatgtgcca gatcgacgtc gatggctcga tccgcttctg
    33901 ctacaagtcc tggcgccagc gtctgggaaa cttcgacgac ggtttctcga acatctggcg
    33961 tggcgaacat taccagcgcc tgcgcgcgac aatggactcg accgcgccct attttccata
    34021 ctgccggacc tgcgccatgc gcatcggcat cgacaatgaa tccgcccatg atcaaagcag
    34081 ccatgccgaa gactacgtga tccccggact cgagcactgg cagaccgatt tcaatgcccg
    34141 tgcggaagaa aatcgcgact ccttcgccga gcgaaaggat cggttgcgag gcaagaaggc
    34201 ctgagccggc cgttactcct gcacgatcct cgcttcagcc atcgccacgt tggcggttcc
    34261 agccctagct gtcatgcgca cgatggcggc ggccccattc cggtcgatcg ccaccacctt
    34321 gccggtcacg acaaaggcct ggtctattcc caacggggcc aggaactgga ccgtgatgtc
    34381 ggtaagcctg atgttccgct tccagagctg gagcaaccga gggaactggg cggcgatcag
    34441 cattcctggc aggaccagtc ccgctaggcc ggcggcaccg gcagccgcct catcgacatg
    34501 gatcggattg tcgtcgccgg cacaggtgac ataggccaga acctgctccc ggctgacagc
    34561 ggccagcggc aggaccggca agggatcgcc cggtttcaga tccagcccca tcaggcaccc
    34621 cggaccgctg gcaccaccag cagcacggag gacatgcgcg ccaccatctt gcccgctgca
    34681 tccgccacga tggcgacaaa ttcgagtttc ggacgcatcg ggtcccggtg cggcagcggc
    34741 ctccagctca aatcgagcgc ataggtacca tcgacctcga tgcgcgagaa ataagcgacg
    34801 ctctggagga agtgcagcgg caggcccttc tccatcccgg cgacagatcg gaccgcggcg
    34861 cggatttccg ggttgccgag ccagatcacc ggataggttg ccggcacttc cggcatggct
    34921 ggcgcctcgc cggagacaag gggcagcgcc accgcatggc gcaagccgct gacgtgttgc
    34981 gaggccgcaa ccacctcgaa acacgcgatg ggcctcaggt cgtcgtccgc aggcatcggc
    35041 tccgccctac aagtccgcgc gcgtgacgac gagaaccgcg ttgattccgc cgaacgcaaa
    35101 ggagttgctc agcgctgcgc cgatctcgcg cgggaaggcg ccctgcgtcg gcaggttgag
    35161 cggacaagcg ggatcgggtt cgaggaagtt gatctggggc ggcacggtct gttccacgag
    35221 cgccttgacg gtgacaatga gttccaggcc accggcggcc cccagaccat ggccgaaaat
    35281 cggcttcgag gaagaaaccg gaatggcatc caacgccgcg ccaaacacgt ttctcagcgc
    35341 cgccgcctca ttgacgtcat tcaggaccgt cgcggtgccg tgcgcattga tgtatccgat
    35401 cgacgccgga tccagttgcg catccgcaag cgcacttcgg atggccgccg tcgccccggc
    35461 gacatcgggc tggatgaaat cgcgcgcgtc gctgctggtg ccgtagcccg cgagcagcgc
    35521 caggggcatg ccgccacgcg cccgaaccgc ttccagcgcc tcgatcacaa ggacgcccgc
    35581 cccctcaccc acgatcatgc cgctacgccc tttcgagaat ggacggcact gatcgctcgt
    35641 cacgacacgc atcgactccc agctgcgcat cgccaccggc gtgatcgcag cctcgctgcc
    35701 gccgacaatc gcgcggtcca cgacgccgct gcgcaccagc atcgcgccga tgccgatgga
    35761 ctgggtcgcc gaagcgcagg cactggtaac ggcgaagcaa gggcccgtcg cgccgtaggt
    35821 catgctgacg aaggaagaag ccgcgctcgg catctggcgc ggaatgccca tgggatccgc
    35881 acgcctgttc tcgacatagt gctggtaatg cccctgatcg agcgaggtaa acccgccgac
    35941 acccgtcccg atgatcaccg cggtgcgcgt accggccagt tcctccgcag acaggccgga
    36001 ttgcgctacc gcctcgcgcg ctgccacgtg cgccagttgc gtgaaccggt cgcagcgcga
    36061 catcatggct ggggcgatgt gctgcaacgg atcgaagtcg cgcacctgcc cggcacggtc
    36121 cacacgaagc ttgcagggcc gcgtcatctc gaccgggccg agcgcgggga tgccatcgcg
    36181 acacgccgtc cagaggcgtt cgactcccag gcccgtcgcg caggccgcac ccatcccggt
    36241 gatcgcaacc tgtctcattg ttgtgcttcg gagccctttg cggccttgtt catttccctg
    36301 accgcctcgt tgaccagttc agccatgttg cgggccgagg cgagatccga gttcatcgga
    36361 atataggtat ccatgacgtc ttccagatcc atcaggatcg tgacgacgtc gatggactcc
    36421 agaccgagcg agtccagagt tgcgtcggga gttatccgat cacgctccag attgccgacc
    36481 ttcgccacga catcgataat cttccccagc atctcctggt ccggcatggc cggccattcg
    36541 agcatcaagg gtttttcatt gatcacttca gtcaatttca actccccagt acagtgccag
    36601 gacaccaaat ttgcttcaca tgctactttt ccgcctgaat gtccttctca aacagccgga
    36661 gcgtccgcac ccggcgtgca ttgaattgct caagaatggc gatcaatgga cggttgctgt
    36721 ccagaaccca tcccgcttcg atcgccggta aggtgtagcg ccgcgccaga gtcaccattt
    36781 cgtgcaccag catcattgca actgccgcac cctccaccgt tctcgagagt cgcgccttga
    36841 cgccgaacaa aatcacccgg gctgacgaaa agcgctgcat ctttgcgcgc cacaggaatt
    36901 tcagccagcc gagcgggctg ggactgtggc ccaggtcggc actcatttca gccagattgg
    36961 gcagcataag gacaatgccg agcggctcgc catccttttc gacgataatc ccgtagtccg
    37021 gatgcatgaa cgggcgaagc tgggtcttga acgcggcgag gtctgcctcc aggagcggga
    37081 tgaagcccca gttattttgc caggcatcgt tgtaaacctc gcgcaggata tcgatctcgc
    37141 gcgacggatg gcgtcggtcc agctttcgga tggtgtagtt caggcgccac ttcggcagcc
    37201 gcaatcgcct cgatacctct tccagttctg ccgttccgat cgcgatcttg aagtaaaata
    37261 gatcacgcac cggcccgaac ccacaagctt cgatcagtcg aagcaagtat tcggggtgcc
    37321 agggaaccat gagcatcggc ggctcaccct gcccctcgac gagcagcccg ggctcgccgt
    37381 tcatcgacag cagacacggt ccgcgcacgc gcttcatgcc cctggcgcgc aaccaggctt
    37441 ccgcttcgtg catcagggcc ttgaccacct cggcgtcatc gatcgcatcg aggcagccga
    37501 agaaccctgt tccggcgaat tcggaggctg tcgcggcatg atcgatttgc gccgaaatgc
    37561 gtccagccgg tgtaccgttc cggaacgcca gccagtactg tgcctcgcca tgcgtgaaga
    37621 aggcaccctt tcttggatcg aggttctgcc gacggtcgag cgtcagcgga ggcacatagc
    37681 gcctgtcgcc gcgatagagc agcctcggca aatcgataaa gagcgcaaga tcacgcggcg
    37741 aattgaccgg tgcgacaatg acggacatga caggcttgca tccggttgcg taaaccgccc
    37801 agcattggcc acggctatgc cgaggtcaag gacagggccc ttcttttagc ctaggcgtgt
    37861 ggactcccgc cagacgatgc gctggatcaa acagaaagca acccgcttgt tacgccagga
    37921 cacctccggc atgctccgcc atatcgtcat caccggcgcc agcagcggca tcggccgcgc
    37981 gcttgcggag cactatgccg gccctggcgt gatgctctcc ctgaccggcc gcaacgcgga
    38041 gcggctggct gcggtcgtgc ggggctgcga agcgcgcggg gccatggtgg caagctcgct
    38101 tatcgacgtc actgaccgcg tgaccctggc ggactggctt gaatcgcgtg acaccgtgca
    38161 gtcggtggat gtcgtcgtgg ctgccgcagg catcggcggg gcggaggtcg ttccggaggc
    38221 aaggggcgag tccgcggatc tggccggcag gattttcgcg accaacacgc tcggcgtggt
    38281 caataccgtc gcgccgctgc tggcgcgcat ggtggcgcgc cgcaccggtg ccgttgtggt
    38341 gatcggttcg atcgcaggac gcatcggcct gccgcagtcg ccggcctaca gtgcctcgaa
    38401 agccgccgtg cacatctacg ccgacgggct gcgacgcctt gttgcgtcca gcggcgtctc
    38461 ggtcacgtcc gtcgtgcccg gcttcgtcga tacgccgatg agccgctcgc tcgccatgtc
    38521 gcgcccgttc tgctggagtg ccgaccgcgc ggcgcggcgg attgcccggg ccatcgaacg
    38581 gcgggagcgg cgctgcatct tcccgcttcc gctgcggatc gccacggctt tgcaccattt
    38641 cgtgcccctc gcactccttg atcgtgtgct tggccgggcg aatacggtca ttcgtcccgg
    38701 gcgggccgac ccgtgacctc gaaacgtttt ttctacctcg ttccggccga aacccgtcat
    38761 cgcctcgtga ccgagacgcg gctgcaacac ctgcgacgct tgcttttcga ccggcggcga
    38821 cgctatccga cgggcggcgt caaggtgatc taccagcatt gcgagatgct gctcgatgcc
    38881 gggttcacag cccatccggt gcatctcggc gacttcgtga tcgactggta tgaccatcaa
    38941 tgccggccgc tttcggttgc cgaagcccgc gccatgatgc gggcggacga tgtgctggtg
    39001 gtgcctgagc gcctgcctgc cgtggcagcg cagttcacct gcggcaccaa agtggcgttc
    39061 gtccagaatc acggactcgt cgcggccgcg ctgcaaggcc gtcgctatgc tgacttcggg
    39121 ttcaccgacc tgctgtgttg cggcacgttc gtggccggca cgctggcaga ccaggctccc
    39181 ctgccctgtc acgtcgtcac gaacggcatc gatctcgaca agttcgttcc cgcccccgat
    39241 ctgagacgac cggacactgt cctgatcctc aggcgcaagg cgtcatggcc gctcggactt
    39301 caggccatcg ggcggctgcc gcaggagatc aggacgcgtc ttgcggtacg cgtgctgccc
    39361 aacacagcct cggagacgca gatgatccga cggtatcagg agagcgatat cttccttgcg
    39421 ctgggctttc cggaagggtt tgcgctgccg ccgctggagg ccatggcctg cggctgcgtt
    39481 gtggccggat tcgccggcgg cggcggcatc agccacatgc ggcacgagac cagcgcgctc
    39541 gtcgtgccgg atggcgacgt cactgcccta gcggccgctc tggaacggct ggcccgcgag
    39601 ccggatctca aggaacgcct gcgcgccggc ggtcaggcgg tggctcaggg cttcgggctt
    39661 gcagccatgc gcgcggccgt gcttgccttc gccgagacga tgcagcggcg catgactagc
    39721 tcctgacgct ctgcgccagg atatccacga cgcgatcgat atcggcatcg tcatgcgctg
    39781 ccgtgacgaa caggcggatg cgcggcatgt tgcgcggcac gccgtagacg atcggcgggg
    39841 catagattcc ggcgtccatc aaccgttcgg cagcggccac cgtctcctgc tgattgcgga
    39901 agatgatcgg caccagagct ctaccgatcg cgtcacccgt gttcaggccc gccgcacggg
    39961 cgcgcataag cagtcgttct gcctttcgcg ccgctgcttg cggccgccat ggctcctgct
    40021 tcgcgagact gatcgcggtg cgcgccatgg cggcgaccgc cggcgcgatg ccgacactga
    40081 agaggaaccc cggaagcccg aaacgcagca cgtcgatgag ggcgcgcgat ccggcgagaa
    40141 agccgccgca ggtggcgaat gtcttcgaca gggtacccat gatgaggtca actcgcgccg
    40201 gatccacgcc ggcatgttcg cagatgccgc ggcctgtctc gcccaggatg ccgtgcgaat
    40261 gcgcctcgtc gatcatcagc caggcacggt gccgatcctt gatgtccagg aggcgcacca
    40321 gatcgggaac atcgccgtcc atgctgtaga gaccttcgac gacgatgatg caattggcgt
    40381 gcgcggcccg gtgctcggtc agcagcgcgt tcaagtgttc gagatcattg tggcggaagg
    40441 ccagtgtctt cgcccgcgtg ccgcgcgtgc cggccaggat gctggcatgg gaaaattcgt
    40501 cgcaaaggac cagatcacgc ccgacaaaga aagtgctgat catcgtctcg ttggtgaggt
    40561 acccgctcag cagcgccagc gaagactcgg cgccgacaaa ctcggcaagg tcgcgctcaa
    40621 gaagcccatg cacggtccgt tcgccgccgg caaggcgcga tgccagcgct cccgaaccgt
    40681 gctcccggat cgcagcgatg ccggcttccg cgatgtcagg gtgccgggac aggccgagat
    40741 agtcgtaatt ggcgaagctg acgaaggtct ggccccttgc ctcggccgcc gccttggcct
    40801 cgtccatcgc cttgaagtag aaattgcgcg ccgctccaag ttcatggccg ccgccgaagc
    40861 gaaacagccg ctgcgcatag tcgccgatgg tgtggtatgg ggaatctgtt tgacccaaag
    40921 caatgtcctt ccaattcccc tctgggatcg tattatttgg gtgggctgag aaatcaaccc
    40981 ggaatgccgg cactggagcc gatgctcctt cctgccgggc gggcggcgcg tgttcgtagt
    41041 cagtgggacg attgtggcca ggatactcta tggcgtgcag ggtgacgcag gcggtcacgt
    41101 atcgcgttcg ctcacgatcg cgaggcaact gcacggtcat gaagtcgtgt tcgtcggcgg
    41161 cggacgcgtc gcccaggcga ggcgtcacgg ctatgatgtc gtggacgtac cgatgctggc
    41221 caacgtctac accaacaacc ggctggacct gccggcgacg ctgatggccg gcgcgcgcgc
    41281 cctccgtgat cgcgcccgga tcttcgaccg gctgacaagc ctcattcgcg attttgatcc
    41341 cgacctgatc ctcacggact acgagccatt tctgccccag gctgccaggc tcgccggacg
    41401 ccccagcatc agcatcaatc ggcaacattt cctgacgcat tgcgactaca ccgtgccttc
    41461 aggcgcccag cttgcccgcc tgcttgcgat gcaggccctg cgccggtttc acatgagcgc
    41521 aagccagtac ttcatcgtct ccttcacgaa ggccacagcc aaggaaccaa agaccgccga
    41581 agtgtttcca tccgtgatcc ggccggaagt cgctgtccgc tcgggcagcg acagcggtgc
    41641 cgtgctggcc tacttgcccc tggcctcgcc tgactggatg cgccggactt tcgccggact
    41701 cgaccgcaag gtcatcgttt atggccagaa ccgtgacggt gacgtcggaa acctcagttt
    41761 ccggccccgg acatctgacg ggttcgcaga cgatctggcg cagtcagcgt tcgtcgtctc
    41821 ccatgccggc agcaatctca ttgccgaatc cctgtttctg aaaaagcccg tgctgacatt
    41881 tccgaccgca cggattttcg agcagtacct caatgcgcac ctgctggcgg gtgccggtta
    41941 cggcaagatc ggcagccagc gcaattcggc cgcatccgtt gctgccttcg acagagaact
    42001 ccatctctac cggtcgcgcc tcgagcaata tcgtccgtgg cgcgacacca ccgtcgtcga
    42061 tcgcctttcc gggcttctcg cccgttgacc tggctcaagg cgttccgctg gcatgccgat
    42121 gatggtttga ccctgatcgt tgaccggcat tgcctgcctg caggaccttg cgccggttct
    42181 cgttaagcct gagaattgcg cagaccacag cgccttcatg cgactggtcg tgctcaccgc
    42241 agacgaaagg ataccgtgtc cgcccttgct gcacacgtag ccaccgtggc caagcttgca
    42301 cccagcttgc tggcgcaccc cggctatagc tacgagaccg ccaagcttct gctcagccgg
    42361 ctgacgctcg cgatgaagac gcctgcagac tatcggggtg ggcagatccg gcaggtcagc
    42421 atccggatca ccgacctgtg caacctgcgg tgtcacacct gcggacaatg gggcgacaac
    42481 ggctatcttc tcggccagcc gatgcgcgaa ctgctgcgcg ccgaagtgcc ggtggatcgt
    42541 tacatcgcgc tgctgcacga tctggcggcc aggggacatc gtccgggcct ctatctgtgg
    42601 ggcggggagc cgatgctcta ccgcggcgcg gtgtgcctga tcgaagaggc ggcgaaactc
    42661 ggcatgccga ccagcatcgc caccaacgga accaaggtgg ccgaaaacgc cgaggcgctg
    42721 gtgcgtgccc cgatgttcat cgcccaggtt tcgatcgacg ggcacaacag cgacatccac
    42781 aatgcctgcc gtcctggggc caacccctcc accgacaact tttcaacggt cgtcggtgct
    42841 ctcgacgccc tcaaggcgga gcgcaaacgc cagggcggga agctgccgat tctcgccgga
    42901 ttgtgcacga tcaaccagcg aaacgcggat cggctggtcg acatctatga cgcgttccac
    42961 gacaaactcg atttcatgat cttctacctg tcgtggtgga tcgatgcgcc ttcggccgcc
    43021 gcacacacga cggaattcca gcgccgcttc ggcacccgac ccgtccggca cgagggctgg
    43081 atcggcggct ggcggccaac ggactattcc ggcctgtccc gacagctcgc gacactggcc
    43141 cgccgcgccc tcagtccgcg cggccccggt gtcctgctcg tcccggacct gacaagcccc
    43201 gaggacctcg aacgctacta caccgaccac ggcgcgacct tcggcttcga tcggtgcacg
    43261 tcgatctatc gcgcggtcga gatcaatgcc aacggcgacg tatcgccctg ccgcgactac
    43321 aacgactacg tcgtcggcaa catcaagacg cacaccatca ccgacttgtg ggacaacgat
    43381 gcctatcgcg cgttccgccg cagcctgaag tgcgatggcc tgatgccggt ctgcacccgc
    43441 tgctgtggca tgatgggcaa ttgaaccagc gcaaggtccc cggacagcat cgatggcacg
    43501 aaggcagtga gcggagcccg cgaacgatgc tgaagacggc ctacatcctg ctgtggtttc
    43561 ccgtcccgac cgagaccttc atcctccgcg aggtggtcaa cctgcggcgc tacggcctgc
    43621 cggtgttcgt gttttcgctt tatgggcgtt ggcgtaagaa cctgtcgcgc gagatgtccg
    43681 aacaggccgc ggatgtggag cggctcggca tgtggagcct gccccggctg atcgcggccc
    43741 tcgttcactg gctgtggcgc aagccggggg aaacgctgca tatcctgcgc cggttcacct
    43801 tccgccggtg gcgtggcctg gagaagaccg gcgagaacct ctgggcactg ctcgcagggt
    43861 tccatctggc ccgccggttc gaggatctgg gaatcgagca tatccatgcg ccctgggcgg
    43921 ccggcccggc gactgcggcg tgggtggcct cgcaactgac agggcggccc tactcgttca
    43981 cggcccgcgc ctgggacatc tatccggccg atgccttgat cggggaaaaa gtggacgccg
    44041 cgacattcgt gcggaccgaa acctccgcca atgtggccca tctcaaggcc atcaccggcg
    44101 cggcggatgc gaaattccgt gtcacctaca acggcatgcc cctgacccga acggtcgatg
    44161 ccccgctccg gatgacaccg ccctacaagc tgctggcgat gggtcggttc gtgccgaaga
    44221 aaggcttcga tgtcctggtg gcggccgccg gcattctcgc cgggcgcggg gtccccttcg
    44281 aattgacctt tgcgggggat ggccgcctgc gcaacgcgct ggagcgacag gcgcgagcgc
    44341 tcggcattgc cgaccgggtc cgctttcccg gttttctgtc gcacgattcc gtcggcgccg
    44401 ccttcgctgg cgccgatgtc ttcgtcatgc cctgtgtggt cgcggcatcg gatgaccgcg
    44461 acggcatccc caccgtgctc atggaagcgc tgatgcatcg cgttccggtg atctcgaccc
    44521 ctgtttccgg gattcccgaa ctcatccggg atggcgaaac cggcctgttg gtccccgaac
    44581 gcgatcccgt tgcgttggct gacgccatcc agcgctgtct gaccgaccgg gatttctccg
    44641 tccgcatcgc tgaacaggga cgagagcagg tgcggcgcca gttcgatgcc gaacgcaacc
    44701 atcgcgccgt gctggcgctg tacgcaacga tcggcaggcc gtgatggcgg ctgccggtcg
    44761 cccgcttcgc ctggtcctgc tgctggagga tctggatgct ggcggcacgc aacgctatgc
    44821 gctctacctt gcgacacaca tcgaccgttc ccggttcaag ccggaaatct ggacgttgcg
    44881 gggcggcagc accttcgcgc cgctcgccgc cgccgccgga attccggtga gcgatctcac
    44941 gcggtcgcgc cgcgtcggtc cggtcgcggt cctgcgcctc atctggcgtc tcctgtttcg
    45001 tcgtccggac atcctctata cgctgaccat catccccaac atctggggac ggctgtttgg
    45061 cggcctgctg cggattcccg tcgtttcggg gtatcgcagc ctacggccgg cgcagctgga
    45121 ccgcctgctg cacccgctgt cgcggcgcat catcgccaat gctccgcaat tgaaggatgt
    45181 gctgaccgat gagctcggcg tgccggcggc gcgcgtcagc gttgttccca acggcgttga
    45241 tctggatcgg ttttcgccgg ttgcccatca tgaatccgaa acacccctta ttgtctgcgt
    45301 tgcgcgcctg gttccaccca aggacctgcc gacgctcgtc gcggcattcg ccatcgtgcg
    45361 agccgccctg ccggaagcac ggctgacgat catcggcgaa ggtccggtgg cgctggcgcc
    45421 tgcgacccaa ctttcggttc tgccggcgcg ctcggacgtt cccgcggtgc tggccagggc
    45481 ctgggtcttt gcgctttcgt cgatcgagga aggtgccccc aacgccatcc tcgaagcaat
    45541 ggcgagcggt cttccggtcg tcgccaccga tgcgggcggc atacgggacg tggtcgaaga
    45601 cggcatgacc ggcgtccttg tgccgcgacg cgatccgcag gccctcgcga cagccttgct
    45661 cgacctcctg cgcaaccggg acacccggcg gcgcatgggc gaggcgggac gtcggcgggc
    45721 ggaggagcgc tactcgattg ccgccatggt gcgggcgacc gaggcgctgc tggacgatgc
    45781 cgcggggggc gccgcgcccg gttccggggg agcgctggca tgaacgggcc actgcctccg
    45841 acgatatgga tcgaagtcga tgacatcatt gagtatttca cccggcacga ccagccgaca
    45901 ggtattcaga gggtggtctt cgaactcctg aacgccgcga ggcaacggtc gcggcaggct
    45961 gtccgcctgt gccgcctcaa ccggcgttcc gggcgcttcg aggcgctcga tctgacgggc
    46021 tacgacgcga tgctgacgtg gttgcggcgc gaaggccggg tctaccagaa atacggacgg
    46081 ctccatgcgc gcctcggcat ctgggcgcgc gacctttacc gtctgaggca ttggcgggac
    46141 cccggccgga agctcagtgc gcggcatgac gccagggcag agacgttttt ccgcgaaatc
    46201 cagacaggtg cggtcatcct gtcgctgggc gggccttggc tgaacccgcg tttcccctca
    46261 gcggtccgcg ccctgaagtc gcgcccgggc gttcgcttcg tgcaacttgt gcacgacatc
    46321 atcccgatct cgcacgctga cctgctcgat gcccgggcca ttccgcaatt cgtgacctgg
    46381 ctgcatgaga ccgccccgct cgtcgacttc tatctgacgc cgtcgaggca cgtcgccggc
    46441 gaacttcaga gctacgctgc cgcgcaggga tggccccggc gcagcatcac cgtgatccgc
    46501 ttcggcagcg gcttcgcgcc tgccgcgccg gtcgggcagg agccgctgcc gtgtccggtc
    46561 ggcgagccct atgcgctctg cgtcggcacg gtcgacctgc gcaagaatac ggagttgctg
    46621 gttcagatct gggagcggct cgtggccaag catggccggg atcgggttcc gctgctggtc
    46681 cttgccggcc atcgcggata tcgttcgcaa gcggtcttca cccggttgca ggcaaccggt
    46741 ttcgttcagg gccgcgtgcg gtttcttgag cgcgtctcga acgcggctct cgaagatttg
    46801 tatcgtcgtg ccgccttcac ggtttttccc agcaaggcgg aaggctgggg actcccggtg
    46861 gaggagagct tcgtctacgg ccgtccggcc atcgtctcga ccgcgacatc gctgccggaa
    46921 gtgggcagcg accttgccga atacttcgat ccggccgatg cggaaggcgc atatgcccgg
    46981 atcgaaaggg ccataaccga ccccgcccat ctggaccggc tggcggagcg cattcgcaag
    47041 gacttcaggc cacgcagctg gaacgagacc ttcgatcaga ttgccgaggt gatcggccgc
    47101 gagtgcgccc tgctcgaacg cgggcggcac gacgccgccg agcgcggaac cgggacgtag
    47161 aacgatgccg gcgctttccc tcgtggtctg cctgcatcgc gaaggcgcct ttctggagcg
    47221 catgctcgcc catgcggacg gctgctatga tgacctcgtg gtcgtccatg atggtcctga
    47281 cgaggacgcg gtgcgggaca ttgtcgaagc gcgcggcggg cgcttcttcg aacggccgcg
    47341 ctcctggcag caggaaccgc actggtcatt cgcctgggaa caggcgcgcc acgactggat
    47401 cctgcgctgg gatgcggacg atctgccaag cgatgcgctg gcgacctggc tccgcgcctt
    47461 ccggactgcc cccgaacccg ccgccgaaat atcggcattt acctgcatct atccgctgtg
    47521 ggacgggaaa acggcacgca cccgtcgatg gccgcgccgc atcggcctgc tcaatcgcca
    47581 tcgcgtgcgc cacatcggaa tggcggacca acctcccatt cccgatggcg aggtgacacc
    47641 tcttgaactt gttctgcgcc atgagccgga ccggcccagc tacggtgtgt ggtacaccgt
    47701 gttccgaccg acatcgcgcc gttggcacct cgatatcgcc cgttcgctcc tgggtaagcc
    47761 cacggacttg ccctgctggc gctggaccag cccgatctgg ccgccggcat gggaagcaat
    47821 ccgtcgccac ccgctgcgga ccggcctgcg gcggttgttc acgaccccgt tcaacaacgc
    47881 tcgtgcgatg atacgccacg gcgaatggcc gcgcccatcc atgctggtgt tttcnnnnnn
    47941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    48001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnctggcc cgccatcaaa ggtaaccgac
    48061 gatccggccc gcgtcgtgtc ggtcctcgcg gcgccggctg tctgtctgtg gcgacacagg
    48121 aacctcgcgc gccggcttgt ccgccgcgac atcgaggcgc gctttcgcgg caccgttctg
    48181 ggcaagatct gggccgtcat cgcgccgctg ctgatgcttg gcgtctacac tttcgtcttc
    48241 ggcgtgctga tccagccgca atggcaggcg gccgtgaatg accgtttcac cgtgccgctc
    48301 atctatttct ccggactgat cctattccag ttctttctgg acgtgttctc gcgggcgccc
    48361 gcgctcattc gcgacaacgc ggtctacgtg aagaaagtcg tcttccccgt cgagattttt
    48421 gcctggattg tcgtcggatc ggcgcttttc cggctgctca tcggctttgc gatgctggtc
    48481 ggcttccatc tcctgctgaa agggctgccc cctgtctggc ttgtgatgat tccgctgttc
    48541 ctggcgcccc tggcgctgat ctcggcgggg cttgtctggt tcctggccgg gttgggcacg
    48601 tatgtgcgtg atgtctcgca ggtcgtgcag gtcctcaccc ctgtgatgat gttcatcagc
    48661 cctgtcttct tcccgctttc ggccattccg gagccgacgc ggagcgtgct ttatgccaat
    48721 ccgctttcct tccccatcga agccgtgcgt tcggtgctgt tcggaggcgg tttcacgaac
    48781 tggagcggcc ttgccgccta tgcgcttggc gccgtcatcg tcgccgggct gggctaccgc
    48841 ttcttcatgc gcgttcggcc agggttcgcc gatgtgctct gatgtggtga tcgcggcaac
    48901 ccgtctgggc aagacgttcc gcgttttcac gaagccttcg cagcagcttg gtgcattgct
    48961 cctgccccgg cgcggcgggg gaagcgaact cgcggccctg cgcgacgtca ccttcacggt
    49021 cgaacaaggc gaatccgtcg ccatcatcgg ccgcaatggt tccgggaagt ccacgctgct
    49081 caacgttgtc tgcggcatcc tgacgccgac acacggtacg gtcacgaccc gcggccgcat
    49141 cgttggcatg ctggaactcg gcaccggctt cgatcccgat ttctccggcc gcgagaacgc
    49201 ccgcctgacg gccgcgatct ttggccttgg cgaggaggag atcgctgccc ggcttgagca
    49261 catgacggaa ttcgcggaca tcggtgacgc gatcgagcgg ccggtgcgcg aatattccag
    49321 cggcatggtc gcgcggctgg ccttcgcggt ctgtgcccat gtcgatgccg acatccttgt
    49381 ggtggacgag atcctggcag tcggcgacgc ggcgttccag cagaaatgca tgcgttttct
    49441 acggacgttc aaggcgaaag gcggcacgct tctgttcgtt tcgcatggcg aacatgcggt
    49501 gctttcgctc tgccggcgcg cgctctggct ccatcagggg aaggtgctcg ccgatgggcc
    49561 gagcaaggaa gtctatcacg cctatgccgc ctcgatcgag gagcatgaag gcatgttgtc
    49621 cgcacccgcc gtcgcgctgg gccatggccc cgcgcctgtg cgggcaccgg cgtcgtctcc
    49681 cttccagcct ttccggtacg atccggatgc cagcgcgccc gctttttccg gcacgacgat
    49741 cacccagatc gacctttgcg acgagaatgg cgacaggcgg gtggtgctgg aaggtgggga
    49801 ggagatcgac gttagcgttc gctgcgcatt cgaagagccg gtcagctcgc cagtcgtggc
    49861 gttcctggtt cgcaacaagc ttggccggat cgttttcgcc gatgacaccg cgcaacttgg
    49921 atcggcagcg gcgtcggccg gggctttcct ggcacggttc accctgcccc tgccctacct
    49981 gccgagcggc gactacctgg tcgaggcatt cgtcttcgac aatcgcgcgc cctcgccgca
    50041 gctgctggcg cgcagcatcg accgctgctt tttccagatc cagtcgcgac accccggcgg
    50101 gggacttgcc aatatcgccc cgcgcaggat cgcaatcggt ccggccgagc ccgggacaat
    50161 ggcgccatga cgggggatct ggcgatcatc gccgagggga tcggcaagac ctaccgcatg
    50221 tacccttcgc aaggatcgcg ccttgccgac ctgctgcgcc ctgcgccacg gcgtggcagc
    50281 gccttcagcg cactggacag catcgatctg aaggtccggc gcggcgagtc cgttggggtc
    50341 atcggccgca atggcgcggg caagtcgacg ctgctgcaag tgatcgcggg gatgctgcgg
    50401 ccgagcacag gcagggtcac cgtgaacggg cgtgtcgccg cgctgctgga actcggcgcg
    50461 ggcttcgatc cggacttcag cgggcgcgag aacgtcgcca tggccgcggc gatcctcggg
    50521 ctcagcgata cgcagatcgt cgagcgtttc gaggcaattc tggctttcgc cggcatcgga
    50581 gcagccatcg acaggcccgt ccgggagtat tccagcggca tgtatgcgcg tctcgccttt
    50641 gccgtggccg tgcatgtcga tgccgacatc ctgatcatcg acgagatcct cgcggtcggc
    50701 gatgcggcgt tccaacggca atgccatcgc gccatcgagg ccttcctgtc gcgcggcgga
    50761 acgctgctgt tcgtctcgca tgacgannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    50821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    50881 nnnnnncagg gcacggtctg gcacggcgaa ggcggcggcg agatcgagca tatcgcgctg
    50941 atcggccggg atggcacgcc gctgtcggca acgcatggcg gggacgaggt cgaactgcgg
    51001 atcagctgtc gggcacgccg ggcgctcaac cggccgatcg tcggctatat cctgcgcaac
    51061 gcgaacggag agaacctcgc cggcgacaac acctatctcc gcacgcgcga aagcggattt
    51121 cccgtcgccg ccggcgaaca gttcgtcgcg cggttcatat tgcaattgcc ctacctcccg
    51181 gccggcgatc acttctttgc cccctcgatc atcgaaggca cgcagcgcga ccatatccat
    51241 ctgcattgga tcgaaaacgc gttgtgcctg cgcgccattg ccagtcctgt aacgctcggc
    51301 gcggttggcg tgccggcgcg caatgtgtcg ttcggacccg tggtcggcta gagcgtccgc
    51361 tccgatccgg caccatccgg atggttgtgc gccagggcgc gcgccagcgt cttgtgggtc
    51421 tggcgcacat gcaacggatc gagcgccgcg atcgccgcga cggcctgcga cacaagcgtc
    51481 gcgttatcgg cggtttgcct ggtgcgcgtc gcttgcacct gttccaacgc gcgccaggcg
    51541 cgttcggcgc aggcgtccca ggtgaattgc cgcgcccgag cctcgccata ggcagccaga
    51601 tcggcctgga atccggtgtc cgtcagcaca tgctgcatca aggcgacaag acttgcatca
    51661 tcgtccggat tgaacaaggc atcggcccgt ccgatcgctt cgggcaggct ggtccggtcg
    51721 gcgccgatga cgggagctcc gcagcccatc gcttcgagca ccggcaggcc aaagccttca
    51781 tggagcgagg gaaacacgaa cagcacacag gcattgtaga gcccgatcaa gtcgtcgtca
    51841 ggcacgtagc cggtgaacac gacctcgtcg gggtccagcc ccagctccgc cgcctggcgc
    51901 gccagaggct gcaactcccg tggcgcgagg tggcagacga tggcgagttg atgtgcccgc
    51961 ctcagcgctt ccggcaggcc ggcgaagccg cgtatcaagc cggcgatgtt cttccgatga
    52021 tccccgccgc cggtatacat gacgaaggga cgcgcgatcc gatgccgggc aagcaggtcg
    52081 cggacggtct tctcgtcgcg ctcgatcctg cggaagcgat gatcgacacc cgaggaaatg
    52141 gtcataacct tctcggccgg gcacccgagg tgctggatcg cttcccggcg ggtcgactcg
    52201 gagatcgcca gcatcgcttc ggcacgatgc aagtcctcaa gcttgcgcaa ataccaggcg
    52261 cgatgggcag ggtcggtcag atagcggtcg gggtcgagca gcggaatcag atcatgcagc
    52321 gtgaccacgg ttccgtagag gcgggtgaat cggccaatgc ttgtcaccgc gtccaatccg
    52381 ctgccttcga aaaggctagg catatggact agatcggggc tgagtccggc caggaatgcc
    52441 tcatagatga tcgtcgcggc atccgaacgc tcgcctgtct tcggctttcg ccttggcatc
    52501 gtgcctttcg cgtaccagac ccggatggcg tgctcgggca acaggccgtc gaaggccgcg
    52561 cggacaggtt cgatcgtttc cggttcgaaa ctgctcaggg ccagaagcac ctcgtgccgg
    52621 ccgcgattgc gcaccatggc ctgggccagc gccagcgcct ggcgtccgat gccccggttc
    52681 cggctgctgg ctgtctgcaa tgcctgtcca tcgatgacga ggcgcatcgg tcgcgcgttg
    52741 ctcatcggcg cagccaggtc caccagagca ccacgacgcc atggcgccgg caactggctt
    52801 ccaacagggc aaggtcaatc gcgatgaccc gctccgaaag gccggcgaca ccagccttct
    52861 gctgcgtccg gcgcgcgaac cagcggcaaa tccggcatga cttcgggttg agcagggaag
    52921 gaagcatgaa cgggacttcg gaacgcggca agaactcctg ggcgccgggt tgcccaggcg
    52981 cgcattctat tccggttcac gacggaaccc aacccgtatc gcggcgtgtc gctcattcgc
    53041 catgacgcct tcacaaaacg cgagccactg cgcggcaacc gcttcgatgc cgtaggcggc
    53101 aaaggcgacc ggccgcgccg cgacgatcct ggccttgaac gcggcatagt cgtccgcgac
    53161 ctcggcaatc ctttccgtcc agtcctggcc gggccggacc agtacctgat cggtcaagcc
    53221 aagcttctcc agcgcgcggc gatgctcagg cgtatcgctc gccaggaacg gcaggccaag
    53281 cccgacgcat ttgaccagcc gattggcgtt cttcgcctgg gtgtgatcgt tcaaggccag
    53341 cggcaacagc gcaagatcgt ggagaaccag ttgctggtcg atggtcctga gatcgtaggg
    53401 cacgtcgccg ccccgcgtga tcctgacgac aggctgcgtg attccggccg ccgcgagcat
    53461 ccaggcgttc tccggcatgc cgaaccagac caaccgaccg cgccagtcca aattgccatg
    53521 gacaaccgag tgatcgagca catcaacggg gttctccagg agatggaccg acccggcggc
    53581 gcgagacagc cggcgccgca agacatcgct gcctgccgtg acggcatcga ccagccgcgc
    53641 gaacacttcg atgcgcgccc gagtcaggag ggactcagcg aattcattgt cgtcgaggtc
    53701 ataaacgacg cgtcgcccac gcagccgcgc cgccagcaga ccggcgaacc gcgtagtttt
    53761 ctggcaaacc agcagatcgt agttcgagcc ggcattgatg ctcgtacgcc aacccaggag
    53821 atggagataa tcctcgatca tcagggcgcg caggcgcgtg ctgggcaaca gcatggtgcc
    53881 ccgcgtatcg aaccggacag catggggcgc gtcggcatcg agcagcccga ccaggcggac
    53941 atcggtcacg tcgatggcgg cagcgccaat ggcgcggatc tcgatgacgc aaggtccgtc
    54001 cgccgtccat tcgaagacgg tccgacagcg ctgccagcgc tcggtcaaca cgaactccgc
    54061 ctggtgaagc aggtgatcgc tcccttccgg gcgcggcgaa acggcactca accggcaaac
    54121 accgccttcg ccggtggcgc gcgctgtcac gcgcagggcg aagcgccgta ccggcggtcc
    54181 cggcacggtg acaggctggc ggagcgccgg gcatccctgc gccgacaacc gcgcggcgaa
    54241 cgacgcatgc ggcaaacccg cgtcatcgaa cggcggggtg gccaagcgat ccggccagcc
    54301 atgaacggga tcagcctccc aatctccggc tccgtcgccg aaggccttcg caaatccgcc
    54361 attgcggacc agatctggcg tggatcgcaa catcgcgctg cccggtcagt cctttgcccc
    54421 aaacacccgc gagcggcctt cggccaggcg cagcagcgcg tcaccccagg ccgaggcccg
    54481 gccgcgcaac agcggaacct gggatcgggc cagcagcaga atgccggcaa ccgcctgcgc
    54541 gacgatggct gcgacaaaca ggaccgtccc cgttgcgccg atcctggcca cgagttcgat
    54601 cagcgtcatg tacaggatgt gaagggccag gatggcggca cagacggcaa tcgccaccag
    54661 gtaaggcgtc cgctgccgcc cggagatggg actgaacgtc aacaacaggc cgaggccgaa
    54721 gagcgtatgc gtcagtgtca ggatcggcgc gcccatgcgc cacacgacct cgcggagcca
    54781 gagttccgtc gcgcgccgtt gcgtggccgc tgtcggcccc tgtgtcagca actggacggt
    54841 tccgacctcg tcaaagaaga cgtagccgcg cgccggggtc gcgaagccgc tatcggggct
    54901 ccagaaggcc cgcgtgtact cgaccctgcg caccgcgttg tcagcagcct tgacgacaat
    54961 ggcctgtccc tcgagcagca gcaggccacg ctccccttcc atgcccacga cgacggccct
    55021 gcgggcgacg taggtttcct cccggtttcc gctcgggtgg ccggcgggct ggcgcttggc
    55081 gatgaagaca tcctgtattt gcgtggcatc gatcttccgg ccgaagaaaa tcgacacgcc
    55141 ttcgttccga aattcgtgaa acacgcccgg ctcaagcatg ctgaggttga agtcgcgcct
    55201 caggaacagc aaggtgtcgt ggatgttctt ggccgaaccc ggcgcaacgg ccatggacaa
    55261 gaccgccacc gccaacatca tgccgcccgc gacaactgcg gccggcatgg cgatcgccag
    55321 aggcgatcgt ccggcggaga acagcgtgga aatcgctccg tccgacagga atcgggaata
    55381 ggttgcgaca atcgccatcg cggtgatctc ggggatcgta tgcaccagga tcatgctggc
    55441 aatggcgtag atcatcagcc agagccgctc gaatggcagc gcaaaggaag gaaggtgaac
    55501 cctcagggaa ataatgatga tcgggaccga cagcgcaacg atgccaaccg cgacgtaaaa
    55561 cgcaagctcg cgcgccatgg aacgcccggc ggttcccagc ctgtaagccg gcatccaaga
    55621 gccggtggcg ggccgttccg acgccgcgcg aaggcttgca ccggcccggg ccggcaggct
    55681 ccgcacctct gtcatgcctg tgccggcgcc gggccggcgg cggttacacc gtttggcccc
    55741 ccgtcaaggc gcattgcggg cagcctcatc gcacctcacc gtttcgctgc gatgcggaaa
    55801 acctaccctg cggataatgt ccgcgtccac cccaccgcac ctgtcgcgtc cgccatgccg
    55861 gcagccacaa cgccagcgtc acgatgtcac gcgccaggca cgcgagcggc gcgcgccagg
    55921 gcagcggccc cccccgcccc gccagcatga tgagttcggc ggtgaaacgg accg
//
LOCUS       DAOMXN010000004        48377 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_521_length_48377_cov_11.843653, whole genome shotgun sequence.
ACCESSION   DAOMXN010000004 DAOMXN010000000
VERSION     DAOMXN010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 48377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 48377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48377
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_521_length_48377_cov_11.843653"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="PK812_00895"
     CDS             <1..350
                     /locus_tag="PK812_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68137.1"
                     /translation="VPRFGPQNFWPIMQGLNLGLRDARQIVDVVGKAARAGRDPGCRQ
                     TLAEYESGRRIDATTRTYGVDLLNRTLISGLLPLDLMRAAGLAAAARIGPFRRLLMRA
                     GMGENLLSLPRTG"
     gene            complement(370..1197)
                     /locus_tag="PK812_00900"
     CDS             complement(370..1197)
                     /locus_tag="PK812_00900"
                     /inference="COORDINATES: protein motif:HMM:NF020478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity EipB family protein"
                     /protein_id="HRJ68138.1"
                     /translation="MLAARRLIAALVAVSTVSITAAQAAGPLLAHRAVYDIALVSEKS
                     KTITAAGGRIAFEFSGNACDGYVLNFRQVTTLDDDEGRSRVMDMRSSTWEGPDGKSFR
                     FTLKNFVNNAPTQASDGTAERAGDGGVSIRLSEPRATRLDLPGEPVFPTEHTRRLLDA
                     AMAGQRTLQLPLYDGSDGGEKFFDTTSVIGRVVEGGDDRLEEAVSKAGLAGQKRWPVS
                     VSYFEGGASGDRTPIYIMSFDLLESGVYSNIKFDFGDFALAARISTLDKLSTDTCQK"
     gene            1350..1823
                     /locus_tag="PK812_00905"
     CDS             1350..1823
                     /locus_tag="PK812_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRJ68139.1"
                     /translation="MSEVEKRLVDLGVTLPAAAAPIANYVPAVISGSLLVVSGQLCFG
                     PDGTLADRHKGKVGDRVAPDAAIEAARFCAINLLAQAKAALGDLDRITRCVRLGGFIN
                     APSGYGALAPIMNGASDLMVAALGERGRHARSTVGMSALPLDAVVEVEAMFEVRP"
     gene            1820..2572
                     /locus_tag="PK812_00910"
     CDS             1820..2572
                     /locus_tag="PK812_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HRJ68140.1"
                     /translation="MSRDLSWVVARPIAHRGLHDRAKGVIENTASAFRAAIARGFAYE
                     CDVQLTADGEAVVFHDFDLDRLTTSTGRVIDHSAAALAQMTMKDTADRIGTVADMFAL
                     TAGKALIVCEIKSANDGDMRLTERVATLARHYSGPLVIKSFDPRVVRHWHSLGTGIPA
                     GIVAMNDYEYPDYAMLSAQEKHALANLLHFADTRPDFVSWRVRDLPCAAPYLCRAALG
                     LPLMTWTVRTPEDREAASRNADQMVFENFIPD"
     gene            2583..3836
                     /locus_tag="PK812_00915"
     CDS             2583..3836
                     /locus_tag="PK812_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRJ68141.1"
                     /translation="MPRSGWRLETISGLAEIDSADWDSCAVPESVSQAPQANDTSREP
                     ADSASEAPESSWQEDPLNPFVSYAFLLSLEESGCVGDSTGWHPLHQVLRDETGRIAAV
                     VPCYAKSHSQGEYVFDHGWADAFERAGGRYYPKLQIAVPFTPVTGPRLLIRNGVDRAA
                     ARAVLAEALLRLPEKLGASSIHATFLQGADAAALETAGWLIRQDRQFHWNNDGYRSFD
                     DFLARLSSRKRKAIRRERAEALAPGIVVEALTGSSISEGDWDAFFAFYMDTGSRKWGR
                     PYLNRRFFSLIAERMAEKIVLIIARREGRPIAGAINFLGRNTLYGRNWGAIEHHPFLH
                     FEVCYYQAIDYAIRRGLARVEAGAQGEHKLARGYVPTTIQSAHGIVHPGFRRAVAEYL
                     AREREHVSEDTRELAALAPFRRDQA"
     gene            3921..4247
                     /locus_tag="PK812_00920"
     CDS             3921..4247
                     /locus_tag="PK812_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68142.1"
                     /translation="MERRELLKGMLLALGAGAVGISLAPTQAAAATPPAPQPDLNPEE
                     TSAMDQEPEANALDDAENDADGQYYYYRRPRRRIYRRRRIVYYRRPRRVYYRPRRVYY
                     RRRYYW"
     gene            4341..4769
                     /locus_tag="PK812_00925"
     CDS             4341..4769
                     /locus_tag="PK812_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HRJ68143.1"
                     /translation="MMSAAYDPNNIFAKILRGELPCHKVYEDEHVLAFMDIMPRCEGH
                     TLVIPKAPARGLLDISPEALAGLALGVQTVAAAVMKATMADGLTILQSNEAAAGQVVF
                     HMHVHIMPRWEGVKLAPHTSQMAPKEALIAAADKIRAALA"
     gene            complement(4772..5122)
                     /locus_tag="PK812_00930"
     CDS             complement(4772..5122)
                     /locus_tag="PK812_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HRJ68144.1"
                     /translation="MSGAVIPVELWPYLTIVLAGFLPTEIWRWLAVLAARDLKEDSPF
                     LHWVRLVATALLMAVVAKLLLHPTGALASVPLWGRLSAVLGGIAVLFATRRSVISAVL
                     TGEVILISAGMLAR"
     gene            complement(5119..5847)
                     /locus_tag="PK812_00935"
     CDS             complement(5119..5847)
                     /locus_tag="PK812_00935"
                     /inference="COORDINATES: protein motif:HMM:NF015549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HRJ68145.1"
                     /translation="MSQPTSEPPKPHPALEGARAAIGLPVILIGVTMVGVGGLARDVG
                     YPVGAAALSTLLIWAGPAQVILFGSVAAGVALPAIALAILVSSMRFLPMTVSILPLAR
                     SRGSRRWQEFLAAHFVSMTTWVEGLRRLPDVPEERRMQFFLGFSTMIIGGATLATIGG
                     FYLYTAVPTAIGAALLFMTPIFFAASLVASARRRDEWTAVVLGALLSLPAFAVLPSGA
                     DFLAVGVVGGSIAFLARSRREPAP"
     gene            complement(5928..6377)
                     /locus_tag="PK812_00940"
     CDS             complement(5928..6377)
                     /locus_tag="PK812_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1489 family protein"
                     /protein_id="HRJ68146.1"
                     /translation="MTLHLLKLCVGCESIRDQEAWIEERRRISGTPGVPYEHKHTTRM
                     VPSRLDEVLRAADGPGSLYWVIKGQIACRQELIDIRPFTDGDGIGRCHFVMKPEVVPV
                     EPRPFRPFQGWRYLRAADAPRDIGGMAEGLADMPEALRRELAGLGLL"
     gene            complement(6374..6553)
                     /locus_tag="PK812_00945"
     CDS             complement(6374..6553)
                     /locus_tag="PK812_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68147.1"
                     /translation="MRSTAALVMVTAASESATCGLQFRLGHHGILAMTRTGGRRFVTN
                     DCLNFICMLQVRRQK"
     gene            complement(6555..8555)
                     /locus_tag="PK812_00950"
     CDS             complement(6555..8555)
                     /locus_tag="PK812_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HRJ68148.1"
                     /translation="MSPPLASVLVANRGEIACRIIRTAKRLGMRTIAVYSDADAQAPF
                     VRMADEAHRIGAAPARESYLRGDIILDVAKKTGAACVHPGYGFLSENTGFARACEAAG
                     IVFVGPPASAIEAMGLKDAAKAIAVKAGVPVVPGYHGENQDPAFLKQKAYETGYPVLI
                     KAVAGGGGKGMKRVDKHAEFEEALASAKREAMNSFSDDRVLIEKYVTAPRHIEIQVFA
                     DKHGKVVHLFERDCSLQRRHQKVIEEAPAPGMPAAMRAAMGKAAVEAARAVGYVGAGT
                     VEFIADGTGGLKDNAFYFMEMNTRLQVEHPVTEAITGLDLVELQFRVAAGEKLPFSQT
                     DLEIDGHAVEARLYAEDPETGFLPSTGKLWALRLPDGSVRVDTGVEEGGEVTAFYDPM
                     IAKIIAHAPTRLEALDALAAALGETMVAGPRTNTAFLKALCEAEGFRKGDFDTGFIDR
                     NLQGLGAVEQPVDAMAVAAAAIDLAEAEANRIAERSWTPQVHNDPWATGDGFALAGTH
                     EHSVDVAVAGTRHRAAIFDDGAERLHVEIPSLGYSGPVSTAGEGAEGLLVVPADDAVL
                     VIRNGRQTEVKLYDPLDVDFDQLDVPEGGAVVKAPMHGKITALLVKTGDAVHKGQKLA
                     AVEAMKMEHALIAPRDGVVAETFGAVGVQVSEGAKLIVIAEA"
     gene            complement(8786..9133)
                     /locus_tag="PK812_00955"
     CDS             complement(8786..9133)
                     /locus_tag="PK812_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRJ68149.1"
                     /translation="MTIQRIESGARMSKAVVHGDTVYLAGIVADDPSKDTADQTRQVL
                     KEIDRLLAAAGTDKSKLLRTNIWLADIKDFAAMNSVWDSWVSPGNTPARATVQAALAT
                     PAYKVEIMVVAAK"
     gene            9310..9684
                     /gene="clpS"
                     /locus_tag="PK812_00960"
     CDS             9310..9684
                     /gene="clpS"
                     /locus_tag="PK812_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HRJ68150.1"
                     /translation="MNRPSRPKSEPSAPSVHAGPGDGGSRDGGTAVATRTKTQTKRPS
                     LYRVLLLNDDYTPMEFVIHILERFFNKSREDATRIMLHVHQNGVGECGVYTYEVAETK
                     VTLVMDFARQHQHPLQCVMEKK"
     gene            9694..12135
                     /gene="clpA"
                     /locus_tag="PK812_00965"
     CDS             9694..12135
                     /gene="clpA"
                     /locus_tag="PK812_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HRJ68151.1"
                     /translation="MPTFSRNLEKALHAALALANERRHEYATLEHLLLALIDDGDAAA
                     VMRACNVDLDALRRTVTTYVDTELQGLATSHKGDPKPTSGFQRVIQRAVIHVQSSGRE
                     EVTGANVLVAIFAERESHAAFFLQEQDMTRFDAVNYISHGIAKRPGMSESRPARGQDE
                     EEDRGGADQGSRSGNAEKGGKKESALQAYCVDLNAKARQGKIDPLIGREAEVNRTIQV
                     LCRRQKNNPMFVGDPGVGKTAIAEGLARRIVTGQVPDVLAGATIYSLDMGVLLAGTRY
                     RGDFEERLKQVIKEIENHPSAILFIDEIHTVIGAGATSGGSMDASNLLKPALAAGTLR
                     CIGSTTYKEYRQHFEKDRALVRRFQKIDVNEPTIPDAIEILKGLKPYFEEFHKLKYTN
                     DAVKAAVELSARYIHDRKLPDKAIDVIDETGASQMLVPAQKRKRTIGIKEIEATIATM
                     ARIPPKTVSKDDAEVLQHLTSTLRRVVYGQDKAIEQLSAAIKLARAGLRDTEKPIGAY
                     LFSGPTGVGKTEVARQLSKALGVELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLL
                     TDGVDQNPHCVLLLDEIEKAHPDLFNILLQVMDHGKLTDHNGKQVSFRNVIIIMTTNA
                     GAADLAKSAYGFTRQKREGDDTEAINRLFAPEFRNRLDAVISFGQLPREVIAQVVDKF
                     VLQLEAQLSDRNVTIELSDDARAWLIEHGYDQTMGARPMARLIQTAIKTPLADEVLFG
                     KLKKGGVVRVVVTEKDGTKTISFDYPEGPVEPKPAPEISKPPASKTRRQVKAKNDRPA
                     DKPRRSTVPRVPLKD"
     gene            12151..12747
                     /locus_tag="PK812_00970"
     CDS             12151..12747
                     /locus_tag="PK812_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HRJ68152.1"
                     /translation="MSLLPLILGLALFFAAHVFTTFRVHRAGVIERLGAGPYRGLYSV
                     ASAVGLALIVFGFGYYRARGYIPLWDPPRWTGHIALTLMPVALVLVASAYMPAGLIKT
                     RARHPFLAGVKLWALAHLLANGDLGSFLLFASFLAYAVYDRIAIKRRGEAPREPRRVS
                     WTTSDTIAVALGALLYSLIAFWLHEVLFGVAAFVPRAS"
     gene            12786..13385
                     /locus_tag="PK812_00975"
     CDS             12786..13385
                     /locus_tag="PK812_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68153.1"
                     /translation="MASFALVLLLAGFLDLSQAKAAAWLSESGSKAWLSGMIGPEDDK
                     KFEEFLNRPRAQKLKVIYLSSPGGYLRPAVAIGMMIRKAGMATAVEADKVLCDSACNV
                     IFAGGAKRYYIRGDKVTEGFSSMTGLGYHPSSQVRDLRSPAVYSDKGTDAMLKYYRAM
                     GQPRAAELAQKAAFNSIFRPNGKTALQLNIATSLSEPPD"
     gene            13509..13865
                     /locus_tag="PK812_00980"
     CDS             13509..13865
                     /locus_tag="PK812_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68154.1"
                     /translation="MIVGMSEGDRYRFEVMLTSDGFIVRARCVESGEVLDNRDRLFRT
                     APAAFAFAELSAMKDAEDSLPRETREAAEAHLASDRAARLFADIRAKLSDSGVSAALL
                     QAWDRRAVITREPLMN"
     gene            complement(13877..14665)
                     /locus_tag="PK812_00985"
     CDS             complement(13877..14665)
                     /locus_tag="PK812_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003599280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HRJ68155.1"
                     /translation="MFYLSKLVWLLMEPSTALTLAVLVGALLCRTRLALWGRRLGIAG
                     AVGLLIGGFTPLSTLILRPLEEHFPPFQDDGIPVAGVLVLGGALQTEVAVGRAQFAVN
                     EAAERFTILPALARRYPEARLVFVGGSGDPLGGIPPEADVVRRVIGDLIPPDRLVYER
                     ASRNTHENAVNSFVQLRPGKGERWLLVTSAWHMPRSVALFRKAGWTVVPFPVDYRSSG
                     SGDDYRPFTSVSQGLRRLDLATKEWVGLVFARLSGQSEALLPER"
     gene            complement(14676..15500)
                     /locus_tag="PK812_00990"
     CDS             complement(14676..15500)
                     /locus_tag="PK812_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRJ68156.1"
                     /translation="MDQIIVAANGARIPAIGLGTWQLTGEVASAAVAHALRCGYRHID
                     TAAAYTNEEAVGQGLRASGIKRDDVFVTTKVWWDSIDDGALQASAEASLKRLGLEQVD
                     LLLIHWPNPAIPLARSIKALNEVKRSGLARHIGVSNFPVALLDEAVRLSTEPLVANQC
                     EYQPLLDQSKVIAACRRHGMAFVSYTPIGRGKIDGTGPIADIARRHSRTVAQVILRWH
                     LQQGLVAIPRSSNPGRIAENFAVWDFALDAADMAAISGLARPDGRIVNIAWAPAWD"
     gene            complement(15561..16451)
                     /locus_tag="PK812_00995"
     CDS             complement(15561..16451)
                     /locus_tag="PK812_00995"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRJ68157.1"
                     /translation="MTRDEVSFPSSGVTCRGWLFRPAGPGPHPAVIMANGMSATKAMG
                     LQSFAESYARRGLAVLAFDYRGLGESDGAERGRLVPQEQHDDTRAAIRWLRAQPGIDA
                     ERIALWGFSFSGGHAVFLASVDPRVKAVAALCPAFSVLGTVQAVAGAAGVARYLARVA
                     PEHDRRATLQPSGEIAIVAPKGQPCLLGAPGAHEWFTAHADAGWRNATTQESVARMAD
                     YAPEAFVELIAPKPILVLAARQDRLVPLVQIESVLARAGEPKRLLVHDGGHFDFLPGG
                     PLHETALEVTGAWLGEALGL"
     gene            complement(16497..17075)
                     /locus_tag="PK812_01000"
     CDS             complement(16497..17075)
                     /locus_tag="PK812_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68158.1"
                     /translation="MNFFVIVFLAIFMTPVAAMDMTIRGDTLVMSGAVVDSDHVRFME
                     LSKTPQFAKVRIVVLQNSPGGRIWPVTEIAREIRKRRLSTLVDASRSFCYSACTGLFA
                     AGVSRHYVNAAGIDPRIPQQRGLGFHEGSSVATGQRQYSGAGTAQMIDIYYEMGVSGA
                     AKIVSNATFRGIYRIPGQRALELGIATSLSPP"
     gene            complement(17176..17472)
                     /locus_tag="PK812_01005"
     CDS             complement(17176..17472)
                     /locus_tag="PK812_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRJ68159.1"
                     /translation="MRNIAGAAAVFVLCTAPAFATSYCEVVKTADGFAALRAAPGASA
                     KQLARMPSGDMVLLREGERSGWVRVTWCRKGMPEQDCQGRVDGWVNKRLVSDCG"
     gene            complement(17563..18735)
                     /locus_tag="PK812_01010"
     CDS             complement(17563..18735)
                     /locus_tag="PK812_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009032091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HRJ68160.1"
                     /translation="MLSNTVREFNFDLGETADAIRETVRDFSQAEIAPRADEIDRSNQ
                     FPRDLWPKMGALGLHGITVEEEYGGAGLGYLEHVVAMEEVSRASASVGLSYGAHSNLC
                     VNQIRRNGSEAQKRKYLPKLISGEHVGALAMSEPGAGSDVVSMKTRADKKGDRYVLNG
                     NKMWITNGPIAETLVVYAKTDPGAGPRGITAFLVEKGMKGFSTAQKLDKLGMRGSDTG
                     ELVFEDCEVPEDNVLGHVGRGVNVLMSGLDYERAVLAAGPLGVMQACMDVVMPYVHER
                     KQFGQAIGEFQLVQGKIADMYVGMNACKAYVYAVAKACDRGETTREDAAGAILYAAEK
                     ATKVALDAIQLLGGNGYINDYPTGRLLRDAKLYEIGAGTSEIRRMLIGRELFGKTG"
     gene            18924..20078
                     /locus_tag="PK812_01015"
     CDS             18924..20078
                     /locus_tag="PK812_01015"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRJ68161.1"
                     /translation="MRFNARHQPQPASRYVVEWTGVEQLAARPEAWNALSARALEPNP
                     FYQPTYLLASARRLVHADIRCVAVYRDAARDSELLGLFPLQQARLTQGGFFPAIDFCR
                     NDFIPLTAPLIDPDDPVGVWESFFLAFQGETPTPRIVLSRYHPQERGTVAALREALAR
                     NSQAFSVIDSSERPSVDTNLEWESYGRTIEAHERSEIGRRQRKLSALGAVTIRTVTQG
                     PDAKAALDEFLRIEGAGWKGLNGTAMAAKASPEQFVRDAFDTPNAEFDVLAIDGKAIA
                     VAINLNAGGVLYTVKTAYDEAYRAQGPGMALGVHQMRKALAGGPYRRIDSCAVPGVPM
                     GKVWRQREPMQWLAFSTTPEIGAGRVDSLAATLRGLGRLKAWIKQQIGRK"
     gene            20085..21425
                     /locus_tag="PK812_01020"
     CDS             20085..21425
                     /locus_tag="PK812_01020"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HRJ68162.1"
                     /translation="MTRVMSLVRRIDRSKLAALAPLAIRIAAAGLAAVLQVVLARLLG
                     ADDYGIFAWAWALVAVGGYATTFGLSQTAVRHLALYAEQKDSAAAQGFLRFSMLCVIA
                     GSSALAAVTALPVLAGAIPWIEANRLVPMLIVLAIIPFFALGDLAEGFARSQGWSVLA
                     LAPGYVMRQLLLILLLPAIWLLGKTPTVTTGLTVALVATAMTALVQMALVLQRLKSVF
                     ARGEIRHDDRPRWLSAAGPVFLADIVQVLRQNVDVLILAAFAPPAQVAVYFAATRVAS
                     LLGLVEFAVGAAAAHRFARIDAKHDAGALDRAVRESAILTFWPTLFAAVLLWLISPLV
                     LALFGEGYRSANELVLVLALGAVLRASVGPADDLLTMQGHGAMNLVAQAVGLATVSLT
                     AIVLVPKLGPSGAALSVLFATGATTFALALASGRRIGIVPLPLPSLILARRQTA"
     gene            21422..22576
                     /locus_tag="PK812_01025"
     CDS             21422..22576
                     /locus_tag="PK812_01025"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRJ68163.1"
                     /translation="MNALLKPVRRPRSSYIAEWTSVDSLARYKDRWRALADRAAEPNP
                     FYSPEYLLSSARDIEKSDIRCMAIYRDATPDADLVGLFPVMHGRLAQGAVIPTLEFYQ
                     NDYICSTAPLIDRDDPVGVCGRFLDLFEGELSRPRTAISRFMPSMRAASKALAEALAE
                     RTYPLEIVSRFERAAVETERGLDSYLSQLSTNRRKDLRRRHRRMEEVGSVTFHTAANG
                     PDLRAALDDFLRIEASGWKGEAGTAMDCQPGTRRLAEHAFLGPDSEIDLLAIDGRAVA
                     ACASIISRGVKFTVKVAFDETYRANSPSVLLDSYMLGQFTRGGRLSRADSCAIPGALV
                     ENLWREKEPVEFQAFAIHPSTSTARVRSLASTLRGFERLKTWMKAQLPRD"
     gene            complement(22602..23315)
                     /locus_tag="PK812_01030"
     CDS             complement(22602..23315)
                     /locus_tag="PK812_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HRJ68164.1"
                     /translation="MEDMNRYSQKGDVKPHPAVARATRQPIQGIDISKWQGEIDWHAV
                     KRAGTKFAFIKATEGGDHVDERFLANWNGAKAAGVARGAYHFVYWCRPAHEQVEWFRK
                     HIPQDPDALPPVLDVEWNGHSKTCPKRVPRDVALEKIRLMLTELEQHTGKKPIIYTDI
                     PFHKDVLEGQFSDYPYWIRSTAAEPHERYTSRPWTFWQFTTTGRVPGIRGDVDRNAFY
                     GSERQWKAFVEQGELIAGH"
     gene            complement(23447..24859)
                     /gene="mgtE"
                     /locus_tag="PK812_01035"
     CDS             complement(23447..24859)
                     /gene="mgtE"
                     /locus_tag="PK812_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HRJ68165.1"
                     /translation="MAEAVQKEPAALGFPEEFRNDEGEVRAEFVALVSAAIEGAHRER
                     LAALVEDLHEADLGDLIEALETDERPRLIELLGRDFDYAALTEVDDAVREEILEEIPN
                     EAIAEGVRDLDSDDAVYILEDLAEEDQEEVLARIPALERVTLERSLDYPEESAGRRMQ
                     ADLIAVPPFWTVGQTIDYMRETEDLPEKFYRIFVVDPGQRIVGVVSLDRLLRAKRPVK
                     IDALMEDVEHIARVDDDQEDVAEAFKRYNLVAIPVVDAEERLVGVLTFDDIVDVIDQE
                     ADEDMRALAGVKADEEITDPVFRISRGRFTWLFANMITAFIASSVLKEFQGQIEKMVA
                     LAVLAPIVASMGGNAGTQAMTVTVRAIAERQITSANFLKVILREAAVGCINGLAFAVI
                     AGALAALWFKTFDLGIVIAVAMQLVLVAAALAGVIVPLTLNRLGADPAVSSGPFVTTV
                     TDIVGFLTFLGTATLWFGLA"
     gene            24948..25682
                     /locus_tag="PK812_01040"
     CDS             24948..25682
                     /locus_tag="PK812_01040"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRJ68166.1"
                     /translation="MRALRDALVTLLLVLAASPIARACGPDALGTARTLSLDTSGGPQ
                     FGLKQYARTLPLGDREVVLTFDDGPHHGVTPRVLDALARECVKATFFLIGRNAAALPG
                     LTARIARDGHTLANHTFSHPWTIDRLSHAAALADLDRGEDAIRMASGARVARFVRFPG
                     FVETPALLSEMTRRNVAVFGTDVWASDWNVMAPDVQLRLVLGRIERARRGIVLFHDIK
                     DQTAAMLPAFLRELKRRGFRVVHVTG"
     gene            25736..26482
                     /locus_tag="PK812_01045"
     CDS             25736..26482
                     /locus_tag="PK812_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68167.1"
                     /translation="MVGKLRNPALAAVCLATLAAVLATSTPAAAQGTAGIRISGAAKN
                     KPFIVLMPGSGGAIPSHWAGVASAQGFAVVSVDVASIFSGRSLLNVSEQEQVQTVGTA
                     LQAAQQAGLDMQRWAILGLSKGGTVALLSAGASRPAGTAQPSAVFAFYPGSVGWCPNS
                     HGASTRVHIFYGDADGWGTHRGTRDACRRQAGGNVAYHEYPGVHHGFDRGGAARTVTC
                     CGTSWVDQGNPAATAKARGVVAATLRSVRR"
     gene            complement(26537..26621)
                     /locus_tag="PK812_01050"
     tRNA            complement(26537..26621)
                     /locus_tag="PK812_01050"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26585..26587),aa:Leu,seq:gag)
     gene            complement(26691..26957)
                     /locus_tag="PK812_01055"
     CDS             complement(26691..26957)
                     /locus_tag="PK812_01055"
                     /inference="COORDINATES: protein motif:HMM:NF013236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HRJ68168.1"
                     /translation="MARLDKVEIDVSAVVGSCVLTLGTLLRLGRGARLFLDGGADAVD
                     ILANGLPIGQGRLIRGREERLAVEVNAIFERPAVVPEPGQPQAT"
     gene            27016..27696
                     /gene="lipB"
                     /locus_tag="PK812_01060"
     CDS             27016..27696
                     /gene="lipB"
                     /locus_tag="PK812_01060"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HRJ68169.1"
                     /translation="MADGNSGLFSRSDGATVEWRIADGLMPYPEAVAFMEARVAAISS
                     GEAQELVWLVEHPPLYTAGTSAKMTDLVDSRFPVHETGRGGELTYHGPGQRVAYVMLD
                     LNRRKRDVRAFVTALESWLIAALADFNVRGETRAGRVGVWVKRPERGVDFEDKIAAIG
                     IRVRRWVTFHGISLNVEPELKDFSGIVPCGIADPRYGVTSLVDLGIPVTMPEVDSVLR
                     RTFERVFS"
     gene            27733..28341
                     /locus_tag="PK812_01065"
     CDS             27733..28341
                     /locus_tag="PK812_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HRJ68170.1"
                     /translation="MESPDQTAPDFVFDLMPLTLTTYATGQNLIFEPYRKEQRRFGIG
                     SVLRFLAFLALGGIAGLALSYFRVGAELEVYASSIGRSWYLDGFGIVLLLGAALLFGI
                     GLGLLAVRSGGRSMMLWMMRNSRAMAEPGRIVFSPAGLTATAASGSRQFPWRTITGYR
                     RARGHWFVFVDTVSFYWLDEDMVADPVAFASYLDRHIATATP"
     gene            complement(28331..29548)
                     /locus_tag="PK812_01070"
     CDS             complement(28331..29548)
                     /locus_tag="PK812_01070"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HRJ68171.1"
                     /translation="MLFLPHLLKRFIRKGRLVVIDHTGARHVFGSGEGGHSVTLRLGD
                     ARVEREIFFNPELKAAEAYMDGRLDFEEGSSVHDLLMLFAINREGLGAHPLQKALRRV
                     WMSLRSRHQANPVSKVSDNASRHYDLSEEFYSLWLDESMTYTCAYFETPETPLAEAQL
                     AKLRHVAAKLALKPGMRVLEIGSGWGGLAIYLAKTCGVHVTGLNVSPRQLAESRRRAE
                     AAGVGDRVAFVEQDYRIMEGAFDRIVSVGMMEAVGIDYFDAYFGAIKRLLKPGGYGLI
                     HCMGRMAPPGTTSPFIRKYIFPGGYVPSMSEVFASTERVGLWVSDCEFLRLHYYWTIK
                     HWRQNFAAVRPKVVAMMGERFARMWEFYLSAVELGFLTGSNMVFQLLISAGRADVPVT
                     RDFMFEEEARLRG"
     gene            complement(29617..30072)
                     /locus_tag="PK812_01075"
     CDS             complement(29617..30072)
                     /locus_tag="PK812_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HRJ68172.1"
                     /translation="MPLYFAYGSNMDVTAMADRCPASRAIGLARLPRHRFLINVDGYA
                     TVVRDARGTVHGLLWDLALRDVQALDRYEGVAQGLYTKVVQPVLTSAGPRQALVYIGR
                     SAQPGLPKPGYLEGVVAAAKAAGLPAGYVAGLDGSPPPAGPKALGRLVR"
     gene            complement(30093..31037)
                     /gene="secF"
                     /locus_tag="PK812_01080"
     CDS             complement(30093..31037)
                     /gene="secF"
                     /locus_tag="PK812_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HRJ68173.1"
                     /translation="MKKLRIIPDGTKFPFMRFRRISFPFSALISVIAIALFFSVSMNF
                     GIDFTGGTLIEMRAKSGKVDIHQVRTTAEKLGLGEVEVQEFSTNAEVSMRFVLQPGGD
                     KGQQVVVEKARAAFINDFEFRRVEVVGPRVSGELVQAGTLGVVVSVFGVLIYLWFRFE
                     WQFAVGAVIATLHDLVMTIGFFAATQIEFNMTSIAAILTIVGYSLNDTVVVFDRIREL
                     LRRYKKMPIEELLDLAMNQTLARTFLVSFTSMLALVALVIFGGNVISSFSWAMLFGVV
                     VGTYSSIFIASPVLIYLGLKVGAEEGKPAPSGAASKAA"
     gene            complement(31050..32657)
                     /gene="secD"
                     /locus_tag="PK812_01085"
     CDS             complement(31050..32657)
                     /gene="secD"
                     /locus_tag="PK812_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019903889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HRJ68174.1"
                     /translation="MLRFSKLKTLAVVALALASILYSLPSAFSPAQQLAWEKSLPTWA
                     PKWLIPHKGITLGLDLQGGTHVVLEVDQTALIRQMVQQLQADIRGIFRDARVAPTGGV
                     PAQPRGAAVRVPNAEDRAKVGPKLKELAQPIDAARLGAGVRNLEVVEQGELFTFAFTE
                     PGINDRTRRAVDQSLDVFRRRVDPEGTKEISIQRQGIDRVLIQVPGLQDPTELREILG
                     KVAKLEFRLVAEPGANPADTETLPSDEPGRGTVTVERRVMVDGADLTSAEPGFDQRSS
                     EPIVNFRFNINGARRFGQVTTENVGKPFAIILDGKVISAPVIRSPITGGQGQISGSFT
                     VDSANRLAILLRSGALPAAFTIVEERTVGPGLGQDSIDAGKKASYVAACLVFLFMLAT
                     YGLFGIFASIALAIHILLIFAIMTKLGATLTLPGIAGIVLTIGTAVDANVLIYERIRE
                     ESHLGRTIVSSLEAGFNRAFATIIDSNVTMFIAAAILYFLGSGPVRGFAVTMIVGIIT
                     TVVTAVTMTRMMIALWYWYTKPTKIPF"
     gene            complement(32698..33024)
                     /gene="yajC"
                     /locus_tag="PK812_01090"
     CDS             complement(32698..33024)
                     /gene="yajC"
                     /locus_tag="PK812_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017483888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HRJ68175.1"
                     /translation="MITPAFAQAAGAPGAGDMVAQFAPFVLILIIMYFLLIRPQQRRA
                     KAHQEMINNVRRGDTIVMSSGMIAKVTKVVDDTEVEAEIADNVRVRVVKGMIADVRNK
                     AEPVAN"
     assembly_gap    33130..33229
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<33230..33864)
                     /locus_tag="PK812_01095"
     CDS             complement(<33230..33864)
                     /locus_tag="PK812_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ68176.1"
                     /translation="MSTLILAGVVRTYRQGVEELPILKGVDLTIAPGELVALVAPSGT
                     GKSTLLHIAGLLEKPTSGEVRISDRRCTGLSDAELTRIRREEIGFVYQFHHLLPELTA
                     IENVMMPQLIAGLGKPEAGKRAAEILNYLGLGHRLTHQPAKLSGGEQQRVAIARAVAN
                     APRLLLADEPTGNLDPHTSEHVFGLLSGIVKQSGLAALVATHNIELALKMDR"
     gene            complement(33866..35089)
                     /locus_tag="PK812_01100"
     CDS             complement(33866..35089)
                     /locus_tag="PK812_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013913156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HRJ68177.1"
                     /translation="MLAWRYLRARRREGFISVIAIFSFLGIMLGVMTLIVVMSVMGGF
                     RKELIGKILGVNGHIFVQPLGQPLTDYAAMADRMNALKGVKLAAPLVEGQVLAASGTA
                     STGAMVRGLREGDIKRFAAIANNIREGTLDGFDEGQGIAIGKRMAGYLNVGIGDRVRL
                     LSPKGPSTPFGSAPRQKAYEVAAIFEVGMSEFDSGFVFMPFGEAQLYFDMEGQASVLE
                     LFIEHPDKVDALTQALQEAALAPVALTDWRFRNNTFFGALEVERNVMFLILTMIVLVA
                     AFNIISGLTMLVKDKTRGIAILRTMGATRGAILRIFIITGAAIGVTGTLLGLAGGLIV
                     AENAETLRKFFSWLFSRPLFPPELYFLSRLPAVVDPREVSLIVGMSLVLSLVATIYPA
                     WKAARLDPVEALRAE"
     gene            complement(35158..36480)
                     /locus_tag="PK812_01105"
     CDS             complement(35158..36480)
                     /locus_tag="PK812_01105"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HRJ68178.1"
                     /translation="MRLTRYFLPVLRDTPKEAEIVSHRLMLRAGMIRQESAGIYAWLP
                     LGLRVLNKVNAIVREEQNRSGAIELLMPTIQSADLWRESGRYDAYGKEMLRIKDRHER
                     EMLFGPTNEEMITEIFRAYVKSYKDLPLNLYHIQWKFRDEVRPRFGVMRSREFLMKDA
                     YSFDLDQAGARHAYNKMFVAYLRTFDRLGLKSIPMRADTGPIGGNLSHEFIVLASTGE
                     SEVFCHKDYLDFAVPGVDTDFDNIAGIQGIVDKWTSLYAATSEMHDVAAYEAIPAASR
                     VSARGIEVGHIFYFGTKYSDPMMAVVNGPDGKETTVHMGSYGIGPSRVAAAAIEASHD
                     DAGIIWPEAIAPFKVALLNLRQGDSSCDAACETLYRQLTAKGVDVLYDDREDRPGAKF
                     ATADLIGVPWQLMVGPKGLAEGKVELKRRVGGDRELLSPGDAVARFTA"
     gene            complement(36891..37868)
                     /locus_tag="PK812_01110"
     CDS             complement(36891..37868)
                     /locus_tag="PK812_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRJ68179.1"
                     /translation="MMMTRRAVLGASAAATTAAMLPMSGLPALAGAPPVGKQAPGFFR
                     FKIGDFEITALHDGIVPRQVQQAYIPNTPLEEVQKVMAAQFMATDVTPNHFTVMTVNT
                     GSKLILIDSGFNNNGAPTTGQLAANMAAAGIDPRQIDTVLVSHFHPDHINGLRAKEGA
                     AVYPNAELVVPAKEWAFWMDDARMNNAPEAARPAFGVTRRVFAPVAKDVRQFEWGKEV
                     APGITAIQSDGHTMGHTSFVVSSGTKSLLVVGDACNDPRLFARNPEWHLMFDADKPLA
                     VTSRKKLLDMASADRMQISFYHAAFPSTGFVAKSGAAYDWFPASFTSVL"
     gene            complement(38054..38476)
                     /gene="ndk"
                     /locus_tag="PK812_01115"
     CDS             complement(38054..38476)
                     /gene="ndk"
                     /locus_tag="PK812_01115"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011926284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HRJ68180.1"
                     /translation="MATQRTFSIIKPDATERNLTGAINAVIEKSGLRIVAQKRIHMTR
                     KQAETFYAVHKERPFFGELVEFMTSAPVVVQVLEGENAVQKYRDVMGATNPANAAEGT
                     IRKLFARSMGENSAHGSDSVENAAIEIAQFFAGNEIVG"
     gene            38602..40482
                     /locus_tag="PK812_01120"
     CDS             38602..40482
                     /locus_tag="PK812_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRJ68181.1"
                     /translation="MLRMSGLTYRLGDRLLFDQASATVPSGARAGFVGRNGSGKTTLF
                     RLVGGDIGAEDGDIDLPRGVRMGRVEQEAPGGPESLLDFVLAADTERLSLLNEAETAT
                     DPARIAEIQLRLADIHAHSAPARAAAILHGLGFDAEAQARPCSAFSGGWRMRVALAAV
                     LFTEPDLLLLDEPTNYLDLEGAIWLQEHLARYPHTAIVISHDRDFLDAVTNFTLHLDR
                     GKLTLYRGGYSSFERQRAEAIVLAEKAAAAVAAEKARLQAFIDRFKAKASKASQAQSR
                     VKRLQKLGEAPATAREEGAELTFPNPEKPQSPPLVAFEGVSAGYGEHLVLRRLNLSIT
                     DDDRIGLIGANGNGKSTFVKLIADRLAPLSGNITRAPKLNVAYFAQHQLDELTAEATP
                     YLLVKRRMPEAPEAKVRSRTAQLGFSSAKSDTPIASLSGGEKARLLLGLATFDGPHLV
                     ILDEPTNHLDIPMRDALVRALADYKGAAIIVSHDRHLIDACCDRLWLVADQSVQPFDG
                     DLDAYARLVMSARAKSPDVRSLDLPTPAASDERRARSERRLALGPLRRKIADLEAQMA
                     RLSDLAAKVDVALAAPDAFIKNPEKAAQLAKDRAALAERLATVEEAWLEASADLEAAE
                     GA"
     gene            complement(40537..42684)
                     /locus_tag="PK812_01125"
     CDS             complement(40537..42684)
                     /locus_tag="PK812_01125"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HRJ68182.1"
                     /translation="MAPKPMSGFKRFGSIASRFAEDRRGGVAIMFAGASMAILTLIAG
                     SVELHQRSLGGTELQKAADAAALAAKRTETDSLTTMQRAQARAAGEIAGKRIFDAAIS
                     EASVFDGVARPNATITWLADGSSKVTVKAKLNTLFAGIIGEAFSDVEVQATANFSVPL
                     PTEVALVLDNTASMFMKDGRAQTRFTQLRDSAKLFTHQLFNAAEQSGSKDWLRLSVVP
                     WAATVNVLSEAPRAPDVAGDAAVTPIPDYGSKRPVASPMLRGGRVNVNSALFAPVGWR
                     GCISGSGEAPGPSDASGMIWNALNVPNNSLDTVTIQQGKMMPIPYRRCTYKQEAYECP
                     PPPPPPSSSGSSSSSSSSSWSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSGGMQGFL
                     DDLKTTDAPVQPAAFFGDRFKDADVLQPPVQKANTCYRTVEDRCWTETVMELRCDGSI
                     PSKTYPACEQDIKEGRRNVYSANPIVCANNSQSGACVLAGTERTQNIGACVGDPNEHK
                     WTGAWCPWVPRTDWTKFDPTIGPNLNCPIPMLGLSGNRRQIIETLDRMTPVSGGTHTD
                     VGLRWGMRTLSPDGDWPSFFGLSKPPVKWKGEETKMMILISDGENSQAIDYPGYWGCK
                     GYVNPGCTGSPDQAALDQRTTQWCNYLKNEKKVDLFVIAVNFTNPQAVQLLNNCAGPN
                     KNGVARFYRIDAAELKNVMKIIAGQVVKLRITS"
     gene            42914..44254
                     /locus_tag="PK812_01130"
     CDS             42914..44254
                     /locus_tag="PK812_01130"
                     /inference="COORDINATES: protein motif:HMM:NF016062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HRJ68183.1"
                     /translation="MTYVFTSTPERGSEAPQSVHANAAAPVSLAISLCGLAAAALTLA
                     FCRQSGALRIEAALLVCMAYAGTVLAGETLLLRTPWAASAGLDFARRGFDLARIGTKL
                     LGLYGVFAVLACIYWLFPEYRAGMYQPTWVAAQQLLPYVLILAVPYVACVDRAMKAPE
                     DGLYWFGRLLLLDRDPARWPALRQFALGWIVKGFFTPIMFHGLVQNTETLMRSSFDLG
                     SLTFAQFYALATVCLFWTDVMVACVGYVFALRLFDTHIRSSEPTAFGWFICLMCYPPF
                     QTVLMAQYFAFSAQNAVRLLSDWPLLQMVWGVAALALLVIYTLSTLVFGCRFSNITHR
                     GVIAAGPYRFTKHPAYVTKNLYWWLAHAPFIPVAGAGEALRFCILMVGINVIYYLRSK
                     TEERHLSHDPAYVAYATAMNDKSLFAPVARILPFLRYRSPECGTAHDGPYKGLA"
     gene            44369..46615
                     /gene="rnr"
                     /locus_tag="PK812_01135"
     CDS             44369..46615
                     /gene="rnr"
                     /locus_tag="PK812_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HRJ68184.1"
                     /translation="MPSREDVLAFVARSPGKVGKRDIARHFGVGGGARVALKRLLKDL
                     EADGLLSRRAKTLHRAGELPSMVVAHIAGKDRDGELFAAPVEWDEEQNGRIPRIHVPI
                     LRRQKHAGPAPAVGDHVLLRVERDRSEEDQYTGRIVKVLTQQKDRTLGIFREDKSRGG
                     GRLLPIDKKSRGLEMVIRPGDEQDAEDGDLIAVDVLRDNRLGLRSARVRDVLGSVKSE
                     KAASLIAIHQHDIPHVFPEATLQEAAAAREPTLAGREDWRAIPLLTIDPADAKDHDDA
                     VHAEADDPQTNPGGFILRIAIADVGAYVRPGSMLDREALNRGNSVYFPDRVVPMLPER
                     ISNDLCSLKPGVARAAMALRVVIDAQGRKKSHGFHRVLMRSAAKLAYPQAQAAIDGRP
                     DDVTGPLLETVLKPLWDAYAALKRARAKRAPLDLDLPERKLVLNPDGTLKDVIVPERL
                     DAHRLIEEFMILSNVCAAETLEKANTLLLYRVHDEPSLEKMESLREFLETIGIDLPKS
                     GALKPELFNRILTRVRGTPNEILTNEVILRSQAQAEYSPENYGHFGLALRRYAHFTSP
                     IRRYADLIVHRALIRALDLGTDGLPETTLDQMREIGATISAAERRAMAAERDTVDRLI
                     TFHLADKVGTSFSGRIGGVTRSGLFVRLDGIGADGFVPAATLGADFFRFDEKAHALVG
                     SRTGETYRLGDPVKVRLVEAQPVAGALRFEILSQGRYTTPARGRSSPGKGRHFARGAR
                     QGRRDQSG"
     gene            46620..47081
                     /locus_tag="PK812_01140"
     CDS             46620..47081
                     /locus_tag="PK812_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HRJ68185.1"
                     /translation="MAIEYRVGANSGVDEARDWASGLRRGLSCRCPRCGEGRMFGRFL
                     KVKPACETCGLELSGHRADDMPPYVVMMIVGHILVPLVLLADNAEVDWPMWLHMALWP
                     TLTLVLCLALLQPVKGALIGYQWALKMHGFDPEAATRDPALPAPLPDAPAR"
     gene            47078..47824
                     /locus_tag="PK812_01145"
     CDS             47078..47824
                     /locus_tag="PK812_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRJ68186.1"
                     /translation="MTNPHVETIERKYMKGQHKAVRPRDAATIILIDRRGREPRVLMG
                     LRNEAAKFMPGVFVFPGGRVEIEDGLVPHAGALSARDVEALHKMSGRLSERRIRAFAL
                     AAIRETFEETGLRIGVPGGAALERPGAWRKGTWAEFTETGLMPTIDDLHFLSRAITPP
                     GRNRRFDTRFFVRDVSALAEQVVDRTTPDSELVELKWVPFAETSDLKTVEITKIMLEE
                     LQRQAEQEFAPSLPRPMYRALNGKFQRIQL"
     gene            47832..>48377
                     /locus_tag="PK812_01150"
     CDS             47832..>48377
                     /locus_tag="PK812_01150"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRJ68187.1"
                     /translation="MSETGRALIAAIALVSLAGLGLSAMIPLLSLELERMGVSSAMNG
                     LNAAVGGIANILIAPVTPLLAGRIGVRRMIAGLILIDAAAILLFPLLPDFWAWCLIRF
                     VFGAALGAMFILSEYWITALAPPDRRGLVMGIYASFLAIGFAAGPVVLARAGTSGWAP
                     YLVTSALFLAALAPLALAPKSP"
BASE COUNT         8793 a        15968 c        14993 g         8523 t
ORIGIN      
        1 tcgtcccgcg ctttggcccc cagaatttct ggcccatcat gcaaggcctc aatctcggcc
       61 tgcgcgacgc ccgccaaatc gtcgacgtgg tcggcaaggc ggcgagagcc ggccgcgacc
      121 ccggatgccg tcagacgctg gcggaatacg aatccggtcg caggatcgat gcgaccacac
      181 ggacttacgg ggtcgacctg ttgaatcgca cgctgatctc cggattgctg ccgctggatc
      241 tgatgcgcgc cgcaggcctt gccgccgccg cccggatcgg accgttccgg cgcctgttga
      301 tgcgcgccgg catgggcgag aacctgctgt ccttgcctcg aaccggctga cgcgcgaacg
      361 ccacgccggt tacttctggc aggtgtccgt cgacagctta tccagcgtgc tgatccgcgc
      421 cgccagcgcg aaatcgccga aatcgaactt gatgttggaa tagacgccgc tttccaggag
      481 gtcgaaggac atgatgtaga tcggcgtccg gtctccgctc gcgcctccct cgaaatagct
      541 gacggaaacg ggccaacgct tctggccagc cagcccggcc ttgctcaccg cctcctcaag
      601 tcgatcatcg ccgccttcga ccacccgacc gatcaccgag gtcgtgtcga agaacttctc
      661 gccgccatcg gaaccgtcgt agagtggcaa ttgcagcgtg cgctggccgg ccatggccgc
      721 atccagcagg cggcgggtgt gctcggtcgg aaataccggt tcgccgggaa gatcaagtcg
      781 tgtcgcgcgc ggctcgctga ggcggatcga gacgccgcca tcgccagccc gttcggccgt
      841 gccgtccgag gcttgagtcg gcgcgttgtt gacgaaattc ttcaaggtga agcggaagct
      901 ttttccgtcc ggtccctccc aggtcgagga tcgcatgtcc atcacgcggg accgcccctc
      961 gtcgtcgtcg agcgtcgtca cctgacggaa gttcaggaca tagccgtcgc aggcattgcc
     1021 cgagaactcg aaggcgatcc ggccaccggc cgcggtgatg gtcttcgatt tctcgctgac
     1081 cagggcaatg tcatagaccg cgcgatgggc cagaagcggg ccggcagcct gcgcggctgt
     1141 gatcgaaacg gtggatacgg cgacaagcgc ggcaatcagg cggcgagcgg caagcatgct
     1201 tcagcaaaac tccggcgtga gcgggactcg tagcgtccat tctctaacga aaccagaatc
     1261 gcggcagcaa tgcggctgtc cggcttgcgt ccgcgcctcg gtgcgtcgca gacttcgcgc
     1321 ctgagtcccg caccggagga agtcatccca tgagtgaagt cgagaagcgc cttgtggacc
     1381 tgggcgtgac actgccggcc gccgccgcgc cgatcgccaa ctatgtgccc gccgtgatca
     1441 gcggttcgct gctggtcgtg tcgggccagc tctgcttcgg cccggacggc acgctcgcgg
     1501 atcggcacaa gggcaaggtc ggcgaccgcg tggcacccga cgcggcgatc gaagccgccc
     1561 gattctgcgc catcaacctg ctggcgcagg ccaaggcagc ccttggcgat ctcgatcgga
     1621 tcacccgatg cgtcaggctc ggcggcttca tcaacgcgcc atcaggctat ggcgcgctcg
     1681 ccccgatcat gaacggcgcg tccgacctga tggtcgcggc gctcggcgag cggggccgcc
     1741 atgcccgctc caccgtcggc atgtccgccc tgcccctgga cgccgtcgtc gaggtcgaag
     1801 cgatgttcga ggtgcgccct tgagccgcga tctttcctgg gtcgtcgcca ggccgatcgc
     1861 ccatcgcggc ctgcatgacc gcgcgaaggg cgtgatcgag aacaccgcca gcgccttccg
     1921 tgcggcgatc gcccggggct ttgcctatga gtgcgacgtg caactcaccg ccgacggcga
     1981 ggccgtggtg ttccacgact tcgacctcga ccggctgacg acatccacgg gccgcgtgat
     2041 cgaccacagc gccgccgccc tggcgcagat gaccatgaag gatacagccg accgcatcgg
     2101 cacggtcgcc gacatgttcg cgctgaccgc cggcaaggcc ctgatcgtct gcgagatcaa
     2161 gagcgccaac gatggcgaca tgcgcctgac cgagcgcgtg gccaccctcg cccgccacta
     2221 ttccggcccg ctggtgatca agtcgttcga tccgcgcgtc gtgcgtcact ggcattcgct
     2281 cggcaccggc atcccggctg ggatcgtcgc catgaacgac tacgaatatc cggactatgc
     2341 catgctgtcc gcccaagaaa agcacgccct ggcgaacctc ctccacttcg ccgatacacg
     2401 gcccgacttc gtctcctggc gcgtgcgcga cctgccctgc gccgccccct atctctgccg
     2461 cgccgccctt ggcctgccgc tcatgacatg gacggtcagg acaccagaag accgggaggc
     2521 cgccagccgg aacgccgacc agatggtgtt cgagaacttc attccggact gaggcagcgc
     2581 ccatgccccg atccggctgg aggctggaaa cgatctcagg tcttgccgaa atcgattcgg
     2641 ctgactggga ctcctgcgcc gttcctgaat cggtttctca agcacctcag gcaaacgaca
     2701 cttcacgcga gccagcggat tctgcatctg aagcgccgga atcatcttgg caggaagatc
     2761 cgttaaatcc tttcgtttcc tacgcttttc tgttgtcact cgaagaatcc ggctgcgttg
     2821 gagacagcac gggctggcac cctctgcatc aggtgctcag ggatgaaacg ggacggatcg
     2881 ccgcggtggt cccgtgctat gccaagagcc acagtcaggg cgaatatgtg ttcgaccatg
     2941 gctgggccga tgcgttcgag cgtgccggcg gacgctacta tcccaagctc cagatcgccg
     3001 tccccttcac tcccgtgacc gggccgcgcc tgctcattcg caacggcgtc gaccgggccg
     3061 ccgcccgtgc cgtgctggcc gaggcgctgc tgcgcctccc ggaaaagctc ggcgcatcct
     3121 cgatccacgc gaccttcctg cagggggccg atgctgccgc cctggaaacc gctggctggc
     3181 tgatccgcca ggacaggcag ttccactgga acaatgacgg ataccgctcc ttcgacgatt
     3241 tcctcgcccg cctgtcgtcg cgcaagcgca aggcgatccg ccgtgaacgc gccgaggcgc
     3301 tcgcgcccgg cattgtcgtg gaggcactga ccggctcatc gatctccgaa ggcgattggg
     3361 acgccttctt cgccttctac atggacaccg gctcgcgcaa gtggggccgg ccgtacctca
     3421 accgccgctt cttctcgctg atcgcggaac gcatggcgga aaaaatcgtt ctgatcatcg
     3481 cccgtcgcga aggccgcccg atcgccgggg ccatcaattt tctggggcgg aacacgctct
     3541 atggccgtaa ctggggcgcc atcgagcacc accccttcct gcatttcgag gtctgctact
     3601 atcaggccat cgactacgcc atccggcggg ggctcgcacg ggtcgaggcc ggagcgcagg
     3661 gtgaacacaa gctggcgcgc ggctatgtgc cgacgacgat acaatcggcg cacgggatcg
     3721 ttcatcccgg cttcaggcgg gccgtcgctg agtatctcgc tcgggaacgt gaacacgtca
     3781 gcgaggacac acgcgaactc gctgctctcg cgccgtttcg acgtgaccag gcatgacaac
     3841 ctggcgtgtg cgctggatca ctgcgcgatg ggccgactcg tgtaagctag ggtggggtga
     3901 tttcaacgga ggtaagttcc atggaaagac gcgaactcct gaagggcatg ttgctggcgc
     3961 tcggcgcggg cgccgtgggt atctcgctgg cgccgacaca ggccgccgcc gccacgccgc
     4021 cagcacccca acctgacctg aacccggaag aaacgtcggc gatggatcag gagccggagg
     4081 ccaatgcgct ggacgatgcg gaaaatgacg cggacggcca gtattattac tatcgccgcc
     4141 cccggcggcg catctaccgc cgccggcgta tcgtctacta tcgtcgcccg cgccgcgtgt
     4201 actatcgccc gcgccgggtc tattatcgcc gccgctacta ctggtaacgg caaggcaggc
     4261 ggaggcgggc accctgcccc ctccgtcttg acgcggaccg atcggcctgc ttctcaaggg
     4321 tctcaatccg agaacgccag atcatgtccg cagcctacga tccgaacaac atcttcgcga
     4381 aaatcctgcg cggcgaactg ccgtgtcaca aggtctacga ggacgagcac gtacttgcct
     4441 tcatggacat catgccgcgc tgcgagggcc ataccctggt catcccgaaa gcgccggccc
     4501 ggggcctcct cgatatcagc ccggaagcgc tggccggtct cgcactgggc gtgcagactg
     4561 tcgccgctgc cgtgatgaag gcgacgatgg ccgacggact gaccatcctg cagtcgaacg
     4621 aagcggcggc cggacaggtg gtgttccaca tgcatgtgca catcatgccg cgctgggaag
     4681 gcgtcaaact ggcaccgcac acgagccaga tggcgccaaa agaggcccta atcgcggctg
     4741 cggacaagat ccgggccgcg ctggcctgac gtcagcgtgc cagcattccc gcgctgatca
     4801 ggatcacttc tccggtcagc acagccgaaa tgaccgagcg ccgcgtcgcg aacagcaccg
     4861 cgatgccgcc aagaaccgcg ctcagcctgc cccaaagcgg aacgctagcc agggcgccgg
     4921 tcggatgcag caggagcttg gccacgaccg ccatcaggag cgcggttgcg accagccgaa
     4981 cccaatgcag gaatggcgaa tcctctttca ggtcgcgcgc ggcgagcacc gccagccagc
     5041 gccagatctc cgtgggcagg aaacctgcca gcacgatcgt cagatagggc cagagttcca
     5101 ccgggatcac ggccccgctc atggcgctgg ctcccgccta gaccgcgcca ggaaggcgat
     5161 gctgccgccc accacgccca cggcgaggaa atccgcccct gatggcagga cagcgaaggc
     5221 cggcagcgac agcaacgcgc cgagcaccac ggccgtccat tcatcacgcc gtcgcgcgct
     5281 cgccaccagc gaggcggcga agaagatcgg cgtcatgaac agcagagccg cgccgatcgc
     5341 cgtcggcacg gcagtataga ggtaaaaccc gccgatcgtc gccagcgtcg caccgccgat
     5401 gatcatcgtg gaaaagccaa ggaaaaattg cattcgacgc tcttccggca cgtccggcaa
     5461 gcggcgcagc ccctccaccc aggtggtcat cgagacgaaa tgcgccgcca ggaattcctg
     5521 ccaacggcgg cttccccggc tgcgcgccaa cggcaggatt gatacggtca tcggcaggaa
     5581 acgcatcgaa gacacgagaa ttgcgagcgc gatcgccggc aatgccaccc cggcggcgac
     5641 cgaaccgaac aggatgacct gcgccggccc tgcccagatc agcagcgtcg atagcgccgc
     5701 cgctccgacc ggataaccca cgtcccgcgc aagaccgccg acgccaacca tcgtcacccc
     5761 gatcaggatg accggcaggc caatggcggc ccgcgcgcct tcaagcgccg gatgcggctt
     5821 gggcggttcg gaagttggct ggctcacagg ctgatttttc atagagggct aaggaaatca
     5881 acggatgtcg tgcatggcag acctgccggg gtgaaccagt cagaccttca cagaagcccc
     5941 agcccagcca actcgcgccg cagcgcctcc ggcatgtccg ccaacccctc ggccatgccg
     6001 ccgatatcgc gcggcgcatc agcggccctg aggtagcgcc agccctggaa cggcctgaaa
     6061 ggacgcggct ccaccggcac gacctcaggc ttcatgacga aatggcagcg tccaattccg
     6121 tccccatccg tgaacggtcg gatgtcgatc agttcctggc ggcaagcgat ctgccccttg
     6181 atcacccagt agagcgagcc tggcccgtca gccgctcgca ggacctcgtc aagccgcgaa
     6241 gggaccatgc gcgtcgtatg cttgtgctcg tagggcaccc ccggcgtccc ggaaatccgg
     6301 cgacgctctt cgatccaagc ctcctgatcg cggatcgact cgcaaccgac gcacagcttg
     6361 agcaaatgca gggtcatttc tggcggcgaa cctgcaacat gcagatgaag ttcaggcagt
     6421 cgttcgtcac aaagcgccgc cctccggtcc gcgtcatcgc caggatgcca tggtgcccaa
     6481 gccggaattg caggccacag gtggccgatt cgcttgcggc ggtgaccatg acgagcgccg
     6541 ccgtgctcct catcctaggc ctccgcaatg acaatcagct tcgcgccttc cgagacctgc
     6601 acaccgaccg cgccgaaggt ctcagccacc acgccgtcgc gcggggcgat gagcgcatgt
     6661 tccatcttca tcgcctcgac ggccgcaagc ttctggccct tgtgcacggc gtcgcccgtc
     6721 ttaaccagca gcgccgtgat cttgccatgc atcggggcct tcaccaccgc cccgccctcg
     6781 ggaacgtcca actggtcgaa atccacgtcg agcggatcgt acagcttcac ctctgtctgc
     6841 cggccgttac ggatgacgag aacggcgtcg tcagcaggaa cgaccaacag tccttcggct
     6901 ccttccccgg cagtcgacac cggcccggaa tagccaagcg agggaatctc cacgtgaagc
     6961 cgttccgcac cgtcatcaaa gatcgccgcg cggtggcgcg tgccggcaac cgcaacatcg
     7021 acggagtgct cgtgcgttcc ggccaaagcg aacccgtccc ccgtcgccca gggatcgttg
     7081 tggacctgag gcgtccaact gcgttcagca atgcgatttg cctccgcttc ggccaagtcg
     7141 atcgccgccg ccgccacagc catcgcatcc accggctgtt ccaccgcgcc gaggccttgc
     7201 agattgcggt ctatgaaacc ggtatcgaaa tcgcctttgc ggaacccttc ggcttcacag
     7261 agtgccttga ggaaagccgt attggtgcgc ggtccagcga ccatcgtctc ccccagcgcg
     7321 gcggccagcg catccagcgc ctccagccgc gtgggcgcgt gggcgatgat ctttgcgatc
     7381 atcgggtcgt agaaggcggt gacctcgccg ccttcctcca cgccggtatc cacccgcacc
     7441 gaaccgtctg gcagccgcaa ggcccacagc ttcccggttg aaggcaggaa acccgtctcc
     7501 gggtcctcgg catagagccg cgcctcgacc gcatggccgt cgatctccag atcggtctgc
     7561 gaaaacggca gcttctcacc cgccgcgacg cgaaactgga gctccaccag atcaagaccg
     7621 gtgatcgcct ccgtcaccgg atgctccacc tgaagccggg tgttcatttc catgaaatag
     7681 aaggcgttgt ccttcagacc gccggttccg tcggcgatga actccaccgt gccggcgcca
     7741 acatagccca ccgcgcgcgc cgcctccacc gccgccttgc ccatggcggc gcgcatcgcc
     7801 gccggcatgc cgggcgccgg cgcctcctcg atcaccttct gatgacgacg ctggagcgag
     7861 cagtcgcgct cgaacagatg cacgaccttg ccgtgcttgt cggcgaagac ctgaatctcg
     7921 atgtggcgcg gcgcggtgac gtacttctcg atcagcacgc ggtcgtccga gaaggaattc
     7981 atcgcctcgc gtttggccga ggcgagcgct tcttcaaact ccgcgtgctt gtccacgcgc
     8041 ttcatgccct tgccgccgcc gccggccact gccttgatca gcacgggata gccggtctca
     8101 taggccttct gcttgaggaa ggcagggtcc tgattctctc cgtgatagcc cggcaccacc
     8161 ggtacgccag ccttcaccgc tatggcttta gccgcgtctt tcaggcccat agcctcgatg
     8221 gcggatgccg gcgggccgac aaagacaatg ccagccgcct cgcaggcgcg ggcaaaccct
     8281 gtgttctccg agaggaaccc gtagcccggg tgcacgcagg ccgcgccggt cttcttcgcg
     8341 acatcgagaa taatgtcgcc cctcaggtag ctctcccgcg ccggcgccgc cccaatgcga
     8401 tgcgcctcgt ccgccatgcg cacgaaggga gcctgcgcgt ccgcatccga atagactgct
     8461 atggtccgca tgccgagccg cttcgccgtg cggatgatgc ggcaggcgat ctcgccgcgg
     8521 ttggcaacca ggacggaggc gagcggaggg gacatgcggg ctccggaggt gagttcaaga
     8581 tcggcagtcg gcagtcggca gtcggcagtc ggcagtcggc agtcggcagt cggcagtcgg
     8641 cagtcgaaag gtgtcattcc ggattggcga agccaagtcc ggaatccatc tttccaacag
     8701 atagacaccc aagatgaatt ccgggcacgc gccaggcgca cccccggaac gacaccggct
     8761 tgcccgtttc cgactgccta tgggctcact tcgccgcgac caccatgatc tcgaccttgt
     8821 aggccggcgt ggcgagagca gcctgcacgg tggcgcgggc cggcgtgtta cccggcgaca
     8881 cccaggagtc ccacaccgag ttcatggcag cgaagtcctt gatgtcggcg agccagatgt
     8941 tggtgcgcag cagctttgac ttgtcggtac cggccgccgc gagcagccga tcgatttcct
     9001 tcagcacctg gcgggtctgg tcagcggtat ccttggaggg atcatccgcg acgatgccgg
     9061 cgagatagac ggtgtcaccg tgtacaacgg ccttgctcat gcgtgcgccg ctttcgatac
     9121 gctggatggt catgcagatc tctccttggg ttaaaaatgc atcggtgtgg ggttagcgct
     9181 acccaacagg caagtccaca cccggacgca ctggcgaaac acgcatgatg ttcctagatt
     9241 gtatgcagcg ggtgcgcgcg cttgattgcg ccgccaagct gccgcagtct tgaggtctga
     9301 cacgacgcca tgaatcgacc gagccgcccc aagtcagagc cttctgcgcc gagtgttcac
     9361 gccggccccg gcgacggtgg cagccgcgac ggcggaaccg ctgtggcaac acgcacgaaa
     9421 actcagacaa agcgtccctc gctctaccgg gtgctgctgc tcaacgacga ctacacgccg
     9481 atggaattcg tcatccacat tctggagcgg ttcttcaaca agtcccggga ggatgccacg
     9541 cgcatcatgc tgcatgtgca tcagaacggg gtgggcgaat gcggagtcta cacctacgaa
     9601 gtcgccgaaa cgaaagtgac gctggtgatg gactttgcgc gccagcacca gcatcctctg
     9661 caatgcgtca tggaaaagaa gtgagagccg ccagtgccga cattttcccg caatcttgaa
     9721 aaggccctgc atgcggcgct cgcgctcgcc aacgagcgcc ggcacgagta cgcaacgctg
     9781 gagcacctgc tgctcgcgct gatcgacgat ggcgacgcgg ccgcggtgat gcgcgcctgc
     9841 aatgtcgacc tcgacgccct gcgccgcacg gtaacgacct atgtggacac cgagctccag
     9901 ggcctcgcca cctcgcacaa gggtgatccc aagccgacct ccggcttcca gcgcgtcatc
     9961 cagcgtgcgg tgatccacgt ccagtcctcc ggacgcgagg aagtgaccgg ggcgaatgtg
    10021 ctggtcgcca tcttcgccga gcgcgagagc cacgccgcct tcttcctgca ggagcaggac
    10081 atgacgcggt tcgatgcggt gaactacatc agccatggca tcgccaagcg acccggcatg
    10141 tcggaatcgc gccctgcgcg cgggcaggat gaagaggaag atcgcggcgg agccgatcag
    10201 ggttcgcgct ccggcaatgc cgagaagggc ggcaagaagg aaagcgcgct ccaggcctat
    10261 tgcgtcgatc tcaacgccaa ggcgcgtcag ggcaagatcg atccgctcat cggccgcgag
    10321 gctgaggtga accgcaccat ccaggtgctc tgccgccggc agaagaacaa ccccatgttc
    10381 gtaggcgacc ctggcgtcgg caagaccgcc atcgccgaag gactggcgcg ccgcatcgtc
    10441 accggccagg tgccggacgt gctggccggt gcgacgatct actcgctcga catgggcgtg
    10501 ctgctggccg gcacgcgcta tcgcggcgat ttcgaagaac gcctcaagca ggtcatcaag
    10561 gagatcgaga accacccctc ggcgatcctg ttcatcgatg aaatccatac cgtgatcggc
    10621 gcgggcgcga cctccggcgg ctcgatggac gcctcgaacc tgctcaagcc ggcgctggcc
    10681 gccggcacgc tgcgctgcat cggctccacg acctacaagg aataccgcca gcatttcgag
    10741 aaggatcgcg cgctggtgcg ccgcttccag aagatcgacg tgaacgagcc gaccatcccg
    10801 gacgcgatcg agatcctgaa ggggctcaag ccgtacttcg aggagttcca caagctcaag
    10861 tacaccaacg acgcggtcaa ggcggccgtg gagttgtcgg cgcgctacat tcacgatcgc
    10921 aagctgccgg acaaggcgat cgacgtcatc gacgaaaccg gtgcctcgca gatgctggtg
    10981 ccggcccaga agcgcaaacg cacgatcggc atcaaggaga tcgaggccac catcgccacg
    11041 atggcccgca ttcctcccaa gaccgtatcg aaagacgacg cggaggtgct ccagcacctc
    11101 acgtccacgt tgcggcgcgt ggtctacggc caggacaagg caatcgagca actctcggcg
    11161 gcgatcaagc tggcgcgcgc cggcctgcgc gacaccgaaa agcccatcgg cgcctacctg
    11221 ttctcgggcc cgacaggggt cggcaagacg gaggtcgccc ggcagctctc aaaggcgctc
    11281 ggtgttgaac ttctgcgctt cgacatgtcg gagtacatgg agcgccatac cgtcagccgc
    11341 ctgatcggcg cgcctccggg ctatgtcggc ttcgatcagg gcggactgct caccgacggt
    11401 gtcgaccaga acccgcattg cgtgctgctg ctggacgaga tcgagaaggc ccatccggac
    11461 ctgttcaaca ttctcctcca ggtgatggac cacggcaagc tgaccgatca caacggcaag
    11521 caggtctcct tccgcaatgt catcatcatc atgacgacca acgccggcgc ggcggacctg
    11581 gccaagtcgg cctatggctt cacgcgccag aagcgtgagg gcgacgatac ggaggcgatc
    11641 aaccggctgt tcgcgccgga gttccgcaac cgtctcgatg cggtgatctc cttcggccag
    11701 ttgccaaggg aggtgatcgc ccaggtggtc gacaagttcg tgctgcaact ggaagcgcaa
    11761 ctgtccgacc gaaacgtcac catcgaactg tcggacgacg cccgggcctg gctgatcgag
    11821 cacggctacg accagaccat gggcgcgcgg cccatggcgc ggctgatcca gaccgcgatc
    11881 aagacgccac tcgccgacga agtgctgttc ggcaagctca agaagggcgg cgtcgttcgc
    11941 gtcgtcgtca cagagaagga cggcacgaag acgatcagct tcgactaccc cgaaggaccg
    12001 gtcgagccca agcccgcccc tgaaatcagc aagccgccgg cttcgaagac gagacgccag
    12061 gtcaaggcca agaacgatcg cccggcagac aagccgcgac ggtccaccgt gccgcgcgtg
    12121 ccgctgaagg attaaggaaa ggaactcgcc gtgtccctac tgccgcttat ccttggactg
    12181 gcgctgttct tcgcagccca tgtcttcacg accttccgcg tgcaccgggc cggcgtcatc
    12241 gaacggcttg gcgccggccc ctaccgcggc ctttactcgg tcgcatcggc ggtcggactt
    12301 gcgctgattg tcttcggctt cggctactac cgcgcccgcg gctacattcc gttgtgggac
    12361 ccgccgcgct ggaccggcca tatcgcgctg acgctgatgc cggtcgctct ggttctggtt
    12421 gccagcgcct acatgccggc cggcctgatc aagacaaggg ctcgccatcc attccttgcg
    12481 ggcgtgaaac tctgggccct cgcccatttg ctcgcgaacg gggacttggg atcattcctc
    12541 cttttcgcgt cattcctggc ctacgccgtc tatgaccgga tcgcgatcaa gcggcgcgga
    12601 gaagcgccgc gcgaaccgcg ccgcgtgtcc tggacgacca gtgacacgat cgcggtggcg
    12661 ctgggggcgc tgctctattc cctgatcgcc ttctggctcc acgaagtgct cttcggcgtt
    12721 gccgccttcg tgccgcgcgc ttcgtgatgc gcagcgccga gggcggcgta ggcggcatgg
    12781 cgcgcatggc ttcgtttgcg ctcgtcctgc tgctggccgg gtttttggac ctttcgcagg
    12841 cgaaagctgc ggcctggctg tccgagagtg gcagcaaggc ctggctgtcc ggcatgatcg
    12901 gtcccgagga tgacaaaaag ttcgaggagt tcctcaatcg gcctcgcgcc cagaaactca
    12961 aggtcatcta cctgtcgagc ccgggcggtt atctgcggcc cgccgtcgcg atcggcatga
    13021 tgatccgcaa ggccggcatg gccaccgccg tggaagccga caaggtgctt tgcgacagcg
    13081 cctgcaacgt catctttgcg ggcggtgcga agcgctacta cattcgcggc gacaaggtga
    13141 ccgagggttt cagctcgatg accgggctgg gctatcatcc gtcaagccag gtgcgcgatc
    13201 tgcgcagccc cgccgtctac agcgacaagg gcacggatgc gatgctcaag tactatcgcg
    13261 ccatgggtca gcctcgcgcg gcggaactcg cgcagaaggc cgccttcaat tcgatattcc
    13321 gtcccaatgg caagaccgcg ctgcaattga acatcgccac aagcctttcg gaaccgccgg
    13381 actgatccac acgttccggt cggtcgcctg cgactttccc catcctggcc tgtgcgctgc
    13441 cgcacatccg tcatgaagcc gccatggcat ctgtcgcata tgcagacagc aagccccggg
    13501 aggcttacat gatcgtcgga atgtcagaag gcgaccgcta ccgtttcgaa gtcatgctga
    13561 cgagcgacgg gtttatcgtt cgcgcgcgct gcgtcgaaag tggcgaagtg ctcgataatc
    13621 gtgatcgcct gtttcgcacg gcgccggcgg ctttcgcctt cgctgagctt tccgccatga
    13681 aggacgcgga ggattccctg ccacgcgaga cgcgcgaagc ggcggaagcc catctcgcgt
    13741 ccgatcgcgc ggcgcgcctg ttcgccgaca tccgcgcgaa gctttccgac agcggcgtgt
    13801 ccgccgcgct gctccaggcg tgggaccggc gcgccgtcat cacgcgcgaa ccgctgatga
    13861 actgatctgc cgcgcttcag cgctcgggca gcagcgcctc gctctgcccc gaaagccgcg
    13921 cgaacaccag tccgacccat tccttggtcg caagatcaag tcgccgcagc ccctgggaca
    13981 cggatgtgaa ggggcgatag tcatcgccgg agccgctgga gcgatagtcg acaggaaacg
    14041 gcaccactgt ccagcccgcc ttgcggaaca gcgcgaccga acggggcatg tgccaggccg
    14101 atgtcaccag caaccagcgt tcacccttcc ccggccgcaa ctgcacgaag ctgttgacgg
    14161 cattctcgtg ggtgttgcgc gaggcgcgct catacaccag ccggtcaggc gggatcagat
    14221 cgccgatgac ccggcgcacg acgtcggctt ccggcgggat accgccgagg ggatctcccg
    14281 aaccgccgac aaagaccaga cgcgcctcgg ggtaccggcg cgccagagcc ggcaggatcg
    14341 tgaagcgctc ggctgcttca ttcacggcga attgcgccct ccccacggcc acttccgtct
    14401 gcagcgcacc acccaggacc agcacaccgg ccaccggaat gccgtcgtcc tgaaacggtg
    14461 ggaaatgctc ctccagaggc cggaggatca gcgtggacaa gggcgtgaac ccgccgatca
    14521 gcaagcccac tgcgccggcg atgccgagcc ggcgccccca gagcgccaac cgggttctgc
    14581 acagcagcgc gccgacaaga accgccaaag tcagcgcggt cgagggttcc atcaggagcc
    14641 agacaagctt tgacagatag aacattgcgc gcgcatcagt cccaggcagg cgcccacgcg
    14701 atattcacga tgcggccgtc cggacgggcc agcccggaaa tggcagccat gtccgccgcg
    14761 tcgagcgcaa aatcccagac agcaaagttc tcggcgatcc gaccggggtt cgatgaacgc
    14821 ggaatcgcga ccagtccctg ctgcagatgc cagcgcagga tgacctgcgc gaccgtcctc
    14881 gaatgccgcc gggcgatgtc ggcgattggt cccgtcccgt cgatcttgcc gcggccgatc
    14941 ggcgtatagg aaacaaaggc catcccatgc ctgcggcagg cggcgatgac cttcgactgg
    15001 tcgagaagcg gttgatactc gcactggttg gcgacaagcg gttccgtcga cagccgcacc
    15061 gcttcgtcaa gcagagccac cgggaagttg gacacgccga tatgccgcgc cagaccgctg
    15121 cgcttgacct cgttcaaggc cttgatcgag cgcgccagcg ggatcgccgg gttcggccag
    15181 tggatgagca gcaagtccac ctgttcgaga cccaaccgtt tcaggctggc ttccgctgat
    15241 gcctgaagcg cgccatcatc gatcgaatcc caccacacct tcgttgtcac gaagacatcg
    15301 tcgcgcttga tgccgctggc gcgcagcccc tgccccaccg cttcctcgtt ggtgtaggcg
    15361 gctgcggtat cgatgtgtct gtagccgcaa cgcagcgcat gggcgacggc agcgctcgcg
    15421 acctccccgg tcaattgcca cgtacccaag ccaatcgcgg gaatgcgcgc accattggcc
    15481 gcgacgatga tctgatccat gccaagcccc tccttgcaag acgaaacgcg gccgcccgcg
    15541 ccgctccgcg accttcgcga tcagaggcct agcgcctcgc caagccacgc gccagtgact
    15601 tcaagcgccg tctcgtgcaa cggtccgccc ggcaggaagt cgaagtgccc tccatcatgc
    15661 accagcagcc gcttcggttc gccggcgcgc gcgagcacgc tctcgatctg aaccagggga
    15721 accagtctgt cctgccgggc agccagcacg aggataggtt tcggcgcgat caactcgacg
    15781 aaagcctccg gcgcatagtc cgccatgcgc gccaccgatt cctgcgtcgt ggcgttgcgc
    15841 cagcctgcgt cggcatgggc ggtgaaccat tcatgggcgc ctggggcccc gagcaggcag
    15901 ggttgtccct tcggggcaac aatggcaatt tcgccgctgg gctggagggt cgcgcgccgg
    15961 tcatgctcgg gcgcgacacg cgcgagatac cgcgcgaccc ctgcagctcc tgccacggcc
    16021 tgcacggttc ccagcacaga aaaggccgga cacagcgccg cgacagcctt cacgcgcgga
    16081 tctacggagg ccaggaacac tgcgtgcccg ccggaaaagg aaaagcccca aagggcgatg
    16141 cgctccgcat cgatccccgg ctgtgcacga agccagcgta tggcggcgcg cgtgtcatcg
    16201 tgctgttcct gcggcacaag tcggccgcgt tcagctccgt cgctctctcc aagcccgcga
    16261 tagtcgaagg cgagcaccgc aagaccacgc ctggcatagc tttcggcgaa ggattgcagg
    16321 cccatcgcct tcgtcgccga catgccgttc gccatgatca ccgcgggatg cggccccgga
    16381 cccgcaggcc ggaacagcca gccccggcag gtcacgccgg aggacggaaa ggaaacctcg
    16441 tcgcgcgtca tgagaacacc cctgctccgg catcaagggc gcaggatggc aggccgtcac
    16501 ggcgggctca agctcgttgc gatgccaagt tcgagcgccc gctggccggg aatgcgatag
    16561 atgcctcgga acgtggcgtt gctgacaatt ttcgccgcac cgctcacccc catctcgtaa
    16621 tagatgtcga tcatctgcgc ggttccggca ccggaatact ggcgctgccc ggtggcgacg
    16681 gacgatcctt catggaagcc caggccgcgc tgctgcggaa tccgtggatc aatgcctgcg
    16741 gcgttcacat aatggcgcga gacacctgcg gcgaacaggc cggtgcaggc ggaataacag
    16801 aagctccgtg aggcatccac aagcgtgctc aaccgccgct tgcggatttc ccgtgcgatt
    16861 tccgtcacag gccagatgcg accacccgga ctgttctgaa gcacaacgat gcgtactttt
    16921 gcaaactgag gcgttttcga cagctccatg aagcgaacgt ggtcggaatc cacaaccgca
    16981 cccgacatca ccagcgtatc cccgcgaatg gtcatgtcca tcgctgcaac tggagtcatg
    17041 aagattgcaa gaaacacaat aacaaagaaa ttcaagtccg gctcctctct tgaatacgtc
    17101 aagaagagaa tgcacagacg gtcgttccag gcaacgccaa acgcaaatat tgagcgtgaa
    17161 ttcgtaagac ggtgctcacc cgcagtcgga cacaagccgc ttgtttaccc atccatccac
    17221 cctgccctgg caatcctgct cgggcatgcc cttgcggcac caggtgacgc gcacccagcc
    17281 agagcgctcg ccctcgcgca acagcaccat gtcgccagag ggcatgcgcg ccagttgctt
    17341 tgccgaagcg cccggagcgg cacggagggc ggcgaagcca tcggccgttt tcaccacctc
    17401 gcaataggag gtggcgaagg ctggagcggt gcaaaggacg aaaacggcag cggcgcctgc
    17461 aatgttacgc atgaatacct cccggttcag atggtcagcg atagcgctga ctatcgcccc
    17521 ttcagtgcgc gcgcgcacaa tgttgcgtgc agacgggcct gatcagcccg tcttgccgaa
    17581 cagctcgcgt ccaatcagca tgcgacggat ttcgctggtg cccgcgccga tctcgtagag
    17641 tttcgcgtcg cgcagcaggc ggccggtggg ataatcgttg atgtagccgt tgccgcccag
    17701 aagctggatc gcgtcgagcg ccactttcgt ggccttttcg gcggcgtaga gaatggcgcc
    17761 ggccgcgtcc tcgcgggtgg tctcgccccg gtcgcaggcc ttcgccacgg catagacgta
    17821 ggccttgcag gcattcatgc ccacatacat gtcggcgatc ttgccctgca caagctggaa
    17881 ttcgccgatg gcctggccga actgcttgcg ctcatggaca tagggcataa ccacatccat
    17941 gcaggcctgc atgacaccga gcggtccggc ggcgagcacg gcgcgctcat agtcgagacc
    18001 gctcatcagc acattgacgc cgcgcccgac atggccgagc acgttgtctt ccggcacctc
    18061 gcagtcctcg aacacgagtt cgcccgtgtc ggagccgcgc atgccaagct tgtccagctt
    18121 ctgcgccgtg gaaaaccctt tcatgccctt ttcaacgaga aaggccgtga tgccgcgcgg
    18181 cccggcgccg ggatcggtct tggcatagac aaccagtgtc tccgcgatgg gaccgttggt
    18241 gatccacatc ttgttgccgt tgagaacata tcggtcgccc ttcttgtccg cgcgcgtctt
    18301 catcgacacg acatcggatc ctgcgcccgg ctcggacatg gccagcgctc cgacatgctc
    18361 gccggaaatg agcttcggca gatacttgcg cttttgcgct tcactgccgt tgcgccggat
    18421 ctggttgacg cagaggttcg aatgcgcccc ataggaaaga ccgaccgaag cggaagctcg
    18481 gctcacttcc tccatcgcca cgacatgttc gaggtagccg aggccggcgc cgccgtattc
    18541 ctcttccacc gttatgccat gcaggccgag cgcgcccatc ttgggccaaa gatcacgcgg
    18601 gaactggttt gagcgatcga tttcgtcggc gcgcggcgcg atctcggcct gggagaaatc
    18661 ccgcacggtt tcacggatcg cgtcggccgt ctcgccaagg tcgaagttga attcgcgaac
    18721 ggtgttggac agcatgacaa ctccatggtg cggtatggca tcgttgcccg aatccgtagc
    18781 agtcccgcgc gccacttcca actagccttc ggatgcagaa accagcaacg ctcccttaac
    18841 gctggaacca agcagcgctc gctccttaag cccatcgtta gggccgtgcg gcaaggtcgc
    18901 agcccagggg aacagggaac tgcatgcgct tcaacgcccg ccatcagccg caaccggctt
    18961 cgcgctacgt cgtcgaatgg acgggcgtgg aacagcttgc cgcgcggccc gaagcctgga
    19021 acgctctcag cgcgcgcgcg ctggaaccga acccgttcta tcagcccacg tatctgctgg
    19081 cgtccgcgcg ccggctcgtc cacgccgaca tacgctgcgt cgccgtctat cgcgacgctg
    19141 cgcgcgacag cgagcttctt ggcctgttcc cgctccagca ggcgcgcctc acgcaaggcg
    19201 gattcttccc cgccatagat ttctgccgca acgacttcat ccctttgaca gcccctctga
    19261 tcgatccgga cgatccggtc ggggtctggg aaagcttctt cctggccttt cagggggaga
    19321 cacccacgcc cagaatcgtt ctgtcacgct atcacccgca ggagcgcggc accgtcgcgg
    19381 cgcttcggga ggcgctggcg cgcaacagcc aggcgttcag cgtcatcgac agttccgagc
    19441 ggccatcggt ggacaccaac ctcgagtggg aatcctacgg ccgcactatc gaggcgcatg
    19501 agcggtcgga aatcggccgc cgccagcgca agctttccgc tctgggagcg gtgaccataa
    19561 ggactgtgac gcaaggcccc gacgcgaagg cggcgctcga cgagttcctg cgcatcgagg
    19621 gcgctggctg gaaaggcctg aacggcacgg cgatggcggc aaaggcatcg ccagagcagt
    19681 tcgtgcgcga tgcattcgac actccgaatg cggaattcga tgtgctggcg atcgacggaa
    19741 aggccattgc cgtcgccatc aacctgaatg ccggcggcgt gctctacacc gtcaagaccg
    19801 cctacgatga agcctaccgg gcgcaaggtc cgggcatggc gctcggcgtc caccagatgc
    19861 gcaaggcgct cgccggaggg ccttatcgtc gcattgattc ctgcgccgtt cccggcgtgc
    19921 ccatgggcaa ggtctggcgt cagcgagaac ccatgcaatg gcttgccttc agcacaacgc
    19981 cagagatcgg cgcgggccgg gttgacagcc tcgctgcgac cttgcgcggc ctggggcgtc
    20041 tcaaggcctg gatcaagcag cagatcggac gcaagtaggc ggacatgaca cgcgtgatgt
    20101 ccctggtgcg gcgcatcgat cggagcaagc tggcagcgct tgcgccgctg gcgatccgca
    20161 tcgccgctgc cgggctggcg gccgtcctgc aggttgtgct ggctcgattg ctcggcgccg
    20221 acgactatgg gattttcgcc tgggcctggg cgctcgtcgc ggtcggcggc tatgccacca
    20281 ccttcggctt gagccagacg gcggtacgcc atctcgcgct ttacgccgag caaaaggatt
    20341 cagcggcagc ccaagggttt ctgcgcttct cgatgctctg tgtgatcgcc ggttccagcg
    20401 cgctggccgc ggtgacggcc ttgcccgtcc tcgccggcgc gatcccctgg atcgaagcga
    20461 accggctggt tcccatgctg atcgtgctcg ccatcatccc gttctttgcc cttggcgatc
    20521 tcgcggaagg gttcgcccgc tcgcaaggat ggagcgtcct cgcactggcg ccgggttatg
    20581 tcatgcgcca gttgctgctc atcctgctgc tgccggcgat ctggctgctc ggcaagacgc
    20641 ccaccgtcac gaccggtttg accgtcgcgc ttgtcgcgac cgcgatgact gccctggtcc
    20701 agatggctct ggtcctgcag cggcttaaat cggtattcgc acgcggagag atccgtcacg
    20761 atgatcgacc tcggtggctt tccgccgccg gcccggtctt cctggcggac atcgtgcagg
    20821 tgctgcgcca gaacgtcgac gtcctgattc tggcggcttt cgctccgccg gcacaggtcg
    20881 cggtctattt cgccgccacg cgcgtcgcat cgctgctggg actagtcgaa ttcgccgtcg
    20941 gcgcggccgc agcccaccgt ttcgcgcgga tcgacgccaa gcacgacgcg ggcgctctcg
    21001 acagagccgt ccgggaaagc gcgatcctga ccttctggcc aacgcttttc gccgctgtcc
    21061 tgctgtggct gatttccccc ttggtgctgg cgctgttcgg cgaagggtat cgcagcgcca
    21121 atgaacttgt tctcgtgctg gcgctcggcg cggtgttgcg cgcctcggtc gggccggccg
    21181 acgatctcct gaccatgcag gggcatgggg ccatgaacct ggtggctcag gccgtcgggc
    21241 ttgccactgt aagtctgacc gcgatcgtcc tcgtgcctaa gctcggtccg tctggcgcgg
    21301 cgctttccgt tcttttcgct accggcgcga ccacgttcgc gcttgcgctc gccagcggca
    21361 ggcgcatcgg gatcgtcccc ctgcccctgc cctcactcat ccttgcccgg agacagaccg
    21421 cgtgaatgca ctgttgaaac ccgtccggag accgcgatca tcctatatcg ccgaatggac
    21481 cagcgttgac tcgctcgccc gctacaagga ccgctggcgc gcgttggctg atcgggctgc
    21541 ggagcccaac ccgttctata gtccggagta cctgctctcg agtgcgcggg acatcgagaa
    21601 gtcggacatc cgctgcatgg cgatctatcg cgatgccacg ccggacgccg accttgtcgg
    21661 actattcccc gtgatgcacg ggcgactggc gcagggcgcg gtgatcccga ccctggagtt
    21721 ctatcagaac gactacatct gttcgaccgc gccactgatc gaccgggacg atccggtcgg
    21781 ggtctgcgga cgttttctcg atttgttcga gggcgagctt tcccgccccc gcacggccat
    21841 ttcccggttc atgccatcca tgcgggccgc gtcaaaggcg ctggcggagg ccctggcgga
    21901 acgcacctat cccttggaga tcgtgtcccg attcgaaagg gcagcggttg aaacggaacg
    21961 tgggctcgac agctacctgt cgcagctcag cacaaaccgg cgcaaggacc tgcgtcgacg
    22021 tcaccggcgg atggaggaag tcgggtcggt gacattccac accgccgcga acggaccgga
    22081 cctgcgggcg gcgctggacg acttcctgcg catcgaggct tccggctgga agggcgaggc
    22141 gggcaccgcc atggattgcc agccagggac acgccggctt gccgaacatg cgttcctcgg
    22201 ccccgattcc gagatcgacc tgctcgccat cgatggtcgg gccgtggcgg cctgcgcctc
    22261 gatcatttcg cggggcgtca agttcacggt caaggtcgcc ttcgacgaaa cgtatcgggc
    22321 gaacagcccc tccgtcctgc tcgattccta catgctcggc cagttcacgc gcggcggacg
    22381 tctttcccgc gccgattcct gtgccatacc cggcgccttg gtcgaaaatc tgtggcgcga
    22441 aaaggaaccc gtcgagtttc aggcgtttgc catccacccc tcgaccagca ctgcgcgcgt
    22501 gcgttcgctg gcctcgacct tgcgcggctt tgaaagactc aagacctgga tgaaggctca
    22561 gttgcccagg gactgacgcg cccgccggaa cggcaatgcc gtcagtgccc ggcgatcagt
    22621 tcgccctgct ccacgaatgc cttccactgc ctctcggacc catagaaggc gttgcggtcc
    22681 acatcgccac ggattccagg cacgcgcccg gtggtggtga attgccagaa cgtccacggc
    22741 cggctggtat agcgctcatg cggctcggcg gcggtcgagc ggatccagta cggatagtcg
    22801 ctgaactggc cttcaagcac atccttgtgg aacggtatgt cggtatagat gatgggcttc
    22861 ttgcccgtat gctgctccag ttcggtcagc atcagacgga ttttctccag cgccacatcg
    22921 cggggaaccc gcttcgggca agtcttggag tggccgttcc actcgacgtc caagaccggc
    22981 ggcagggcat cgggatcctg cggaatgtgc ttgcggaacc attcgacctg ctcatgcgcg
    23041 ggccggcacc agtagacgaa atggtaggcg ccgcgcgcca cgccggcggc cttcgccccg
    23101 ttccagttgg ccaggaagcg ttcatcgaca tggtcgccgc cttcggtggc cttgatgaag
    23161 gcgaacttcg tgccggcgcg cttgaccgcg tgccagtcga tctcgccctg ccatttcgag
    23221 atgtcgatgc cctgaatcgg ctggcgcgtc gcgcgcgcca ccgccggatg cggcttgaca
    23281 tcgcccttct gcgaatagcg gttcatatcc tccatggcgc aggaggacag gatgacaagg
    23341 gcgccgatgc ccgccacgag gcggggccac agcgatggct ttcgagtttt ccgtttcatg
    23401 accgagagaa atgcagccaa tttcttaagc gttcgtcaag acttgcctag gcgaggccaa
    23461 accagagcgt ggccgttccc aggaaggtta agaagccgac gatatccgtc acggtcgtga
    23521 cgaacggccc ggacgacacg gccggatcgg cgccgagccg attcagcgtg agcggcacga
    23581 tgacgccggc aagcgctgca gcaacgagca ccagttgcat cgccaccgcg atcacgatgc
    23641 ccagatcgaa cgtcttgaac catagcgccg ccaaagcacc ggcgatcacg gcgaaagcaa
    23701 ggccgttgat gcagccgacc gccgcttcgc gcaggatgac tttcagaaag ttcgccgacg
    23761 tgatctggcg ttcggcgatc gcacgtaccg ttacggtcat ggcctgcgtt ccggcgttgc
    23821 cgcccatgct ggcgacgatc ggcgccagca ccgccaaagc gaccatcttt tcgatctgcc
    23881 cctgaaattc cttcagcaca ctggaggcaa taaaggcagt gatcatgttg gcgaacagcc
    23941 aggtgaagcg gccgcgcgag atgcggaaca ccggatcggt gatttcctcg tccgccttga
    24001 cgccggccag cgcgcgcatg tcctcgtcgg cttcctggtc gatgacgtcc acgatgtcgt
    24061 cgaacgtcag gacgcccacc agccgctcct cggcatcgac gaccgggatc gccacgaggt
    24121 tgtagcgctt gaacgcctcg gcaacatctt cctgatcgtc gtcgacacgg gcgatgtgct
    24181 ccacatcctc catgagagcg tcgatcttca ctggccgctt ggcgcgcagg aggcgatcga
    24241 gcgagacgac gccaacgatg cgctggccag gatcgacgac gaaaatgcgg tagaatttct
    24301 cgggaagatc ctcagtctcg cgcatgtagt cgatggtctg gccaaccgtc cagaacggcg
    24361 gcacggcgat gaggtcggcc tgcatgcgcc gccccgccga ctcctcggga tagtcgaggc
    24421 tgcgctccag cgtgacgcgt tccagcgccg gaatacgcgc caggacctct tcctggtcct
    24481 cttcggccaa atcttcgaga atgtagaccg catcgtcgga atcgaggtcg cgcacgccct
    24541 ccgcgatggc ttcgttggga atttcctcga ggatttcctc gcggaccgcg tcatcgacct
    24601 cggtcagagc cgcatagtcg aaatcgcgtc cgagcagctc gatgaggcgc ggtcgctcat
    24661 cggtttcgag cgcctcgatc aggtcgccga ggtcggcctc gtgcaggtcc tcgacgagag
    24721 ctgcaagccg ctcgcgatgc gcgccttcga tcgcggcgct gaccagagcc acgaattccg
    24781 cgcgcacttc cccctcgtcg ttccggaact cttccggaaa acccaaggcg gccggttctt
    24841 tctgcacggc ttcggccatc ggacacgacc gcgttggagg aggatgctcg actcgtatta
    24901 ggtaagcgct tcggtcgagg caattcggat tgcatggggg aaatcgcttg cgtgcacttc
    24961 gcgacgctct tgtgacgctt ttgcttgtgt tggcggcgtc gcctatcgca cgcgcgtgcg
    25021 gtccggatgc gctgggcaca gccaggacac tctcgctcga cacgagcggt ggaccgcaat
    25081 tcggcctgaa gcaatatgcc cggacccttc ccctcggcga tcgcgaagtc gtcctgacct
    25141 tcgatgacgg gccgcaccat ggcgtgacgc cgcgcgtgct ggacgcgctg gcgcgtgaat
    25201 gcgtgaaggc gacgttcttc ctcatcggcc ggaatgcggc ggcgctgccc ggattgactg
    25261 cgcggattgc gcgcgatggg cacacgctgg caaaccacac cttttcccat ccctggacca
    25321 tcgaccgcct gtcacatgcc gcggcacttg ccgatctcga tcggggcgag gacgcgatcc
    25381 gcatggcctc cggggcgcgt gtcgcccggt tcgtgcgctt tcccggattt gtcgagaccc
    25441 cggccctgct ctcggaaatg acccgccgta acgtcgctgt cttcggcacc gacgtctggg
    25501 catcggactg gaacgtcatg gcgccggatg tgcagctccg gcttgttctc ggtcggatcg
    25561 agcgggcacg tcgaggcatc gtcctgtttc atgacatcaa ggaccagacc gccgccatgc
    25621 tgccggcctt cctgcgcgaa ctgaagcggc gcgggttcag ggtcgtgcat gtgacgggct
    25681 gaaaggttga cacccgccgc cgttctgaaa ggatttccgc aaagtggagg cgtgaatggt
    25741 aggcaaactg cggaaccctg ccttggcggc agtatgtctc gcgaccctgg ctgccgtgct
    25801 cgccacatcg acgcccgcgg ctgcgcaagg aacggcgggg atccgcatct ccggcgcggc
    25861 caagaacaag cccttcattg ttttgatgcc cggttccggc ggcgccattc cctcccactg
    25921 ggcgggcgtt gcgtctgctc agggcttcgc cgtcgtgagc gttgacgtcg cttccatttt
    25981 ttccggacgc tccctgctca acgtctccga acaggaacag gtccagaccg ttggcaccgc
    26041 gcttcaggcg gcgcagcagg cggggctgga catgcagcgc tgggcgatcc tcggcctgtc
    26101 caagggaggt acggttgcgc tgctaagcgc cggcgcctcg cgaccggcag gaacggcgca
    26161 gccttcggct gtcttcgcct tctaccccgg aagcgtgggc tggtgtccca acagccacgg
    26221 cgccagcacc agggtgcaca tcttctacgg cgacgccgat ggctggggca cgcatcgtgg
    26281 tacgcgcgat gcttgcaggc gacaggccgg cggcaatgtc gcctaccatg aatatcctgg
    26341 cgtccatcat gggttcgatc gcggcggcgc cgctcgcacc gtgacttgct gcggtacgtc
    26401 ctgggtcgat caaggcaacc cggcggccac cgccaaggcg cgcggcgtgg ttgcggcaac
    26461 gctgcggtca gtacggcgct aggacgaaaa aagcgacccg aaggtcgcct tttcaacacc
    26521 ggtttcacca gatgaatggt gcggccaaga ggactcgaac ctccacggtg ttacccacta
    26581 gcacctcaag ctagcgcgtc taccaattcc gccatggccg cgcggggggc cggctaaccg
    26641 gcttgctccg aggcgcgctg actaacaaac gcttgccatg atgacaaggc ttaagttgcc
    26701 tgcggctgcc ctggttccgg aacaaccgct ggtctctcga agatggcatt gacttcgact
    26761 gcaagccgct cctcacggcc acggatgagc cgcccttgcc cgatcggcaa cccgttggcc
    26821 aggatgtcaa cggcatccgc gccaccgtcg aggaatagcc gcgcaccccg tccaagccgc
    26881 aacagcgtcc ccagtgtcag gacgcatgat ccgacgaccg cagagacgtc gatttccacc
    26941 ttgtcgaggc gcgccatgcg tggtcttcct ccgagtcgac actttcgcgc caattgttca
    27001 ccctctggtt aaaaaatggc ggacggcaac tcaggattgt tctctcgctc cgatggcgca
    27061 acggtcgaat ggcgcatcgc ggatgggctg atgccctacc cggaggcggt cgccttcatg
    27121 gaggcacgtg tcgctgccat ttcttcaggc gaagcacagg agctggtctg gctggtcgag
    27181 caccccccgc tctatacggc agggacatcc gcgaagatga ccgatctggt cgactcccgc
    27241 tttcctgtcc acgagaccgg gcgcggcggc gagctgacct atcacggacc cggccagcgc
    27301 gtggcctacg tcatgctgga cctgaaccgc cgcaagcgcg atgtccgcgc ctttgtaacc
    27361 gcgctcgaat cctggctgat cgccgcgctg gcggacttca acgtgcgagg agaaacgcgc
    27421 gcgggccgag tcggcgtctg ggtgaagcgg ccagagcgcg gcgttgattt cgaagacaag
    27481 atcgctgcga tcggtatccg cgtgcgccgc tgggtgacgt ttcacggcat ttccttgaat
    27541 gtggaaccag agttgaagga tttctccggc atcgtaccct gcggcatcgc cgatccgcgc
    27601 tatggcgtga cctcgcttgt cgatctcggc ataccggtca ccatgccgga agtggacagc
    27661 gtgctgcgca ggacattcga aagagtgttt tcgtgatcgg gctcggcctg atgcgtgcaa
    27721 gaggaggcca ggttggaatc gcccgaccag acagcgcccg atttcgtctt cgacctgatg
    27781 ccgctgaccc tgacgaccta tgcaaccggc cagaacctca tcttcgaacc ctaccgcaag
    27841 gaacagcgac gcttcggtat cggcagcgtc ctgcggttcc tcgcctttct cgcgctcggg
    27901 ggaatcgcgg ggctcgcatt aagctacttc cgcgtcggcg cggagctcga agtctacgcc
    27961 agttcgattg gccgaagctg gtaccttgat gggtttggca tcgtgctgct tctgggtgcc
    28021 gccttgctgt tcggcattgg gctgggactg ctggcggtgc gctcgggcgg tcgctcgatg
    28081 atgctgtgga tgatgcgcaa cagccgcgcc atggcggagc ccggccgcat cgtcttttcg
    28141 cccgccggac tgaccgctac cgcagcgtcc gggagccggc agttcccgtg gcgcacgatc
    28201 accggctacc ggcgcgcgcg ggggcactgg ttcgttttcg tggacaccgt gtcgttctat
    28261 tggctcgatg aggatatggt cgcggatccg gtcgcttttg ccagctatct cgaccgccac
    28321 atcgcaaccg ctacccccta agccgcgcct cttcctcgaa catgaagtca cgcgtcaccg
    28381 gcacatcggc ccgcccggcc gaaatcagca gctggaacac catgttcgag ccggtgagga
    28441 agccgagttc gactgcgctc aggtagaact cccacatccg ggcgaagcgc tcgcccatca
    28501 tcgcgacgac cttgggccgc acggcggcaa aattctggcg ccagtgcttg atggtccagt
    28561 agtagtgcag gcgcaggaac tcacagtcgg aaacccaaag cccgaccctt tccgtcgatg
    28621 cgaagacctc cgacatcgat ggcacatagc cgccggggaa aatgtatttc ctgatgaacg
    28681 gcgaagtggt tccgggcggc gccatgcggc ccatgcaatg gatgagaccg tatccccccg
    28741 gcttcagcag ccgcttgatc gcaccgaaat aggcgtcgaa gtagtcgatc ccaaccgcct
    28801 ccatcatgcc gacggagacg atgcggtcga acgccccctc catgatccgg tagtcctgct
    28861 cgacaaaggc gacccggtcg ccaaccccgg cagcctctgc acggcggcgc gattcggcga
    28921 gttgacgcgg cgagacattc aggcccgtga catgcacgcc gcaggttttc gccagataga
    28981 tcgcaaggcc gccccagcca gagccgatct ccagcacgcg catgcctggt ttcagagcca
    29041 gctttgccgc cacatgcctg agcttggcca gttgcgcttc ggccagcggt gtctcgggcg
    29101 tctcgaaata ggcgcaggtg taggtcatgc tctcgtcgag ccagagcgag tagaactcct
    29161 ccgagaggtc atagtgccgc gacgcgttgt cggagacctt gctgaccgga ttggcctgat
    29221 ggcggctgcg cagggacatc cagacccggc gcaacgcctt ctgcaaagga tgtgcgccca
    29281 ggccctcgcg gttgatcgca aacagcatca gcagatcgtg gacgctcgaa ccttcctcga
    29341 aatccaggcg cccatccata taggcttcgg ctgccttcaa ctcgggattg aagaaaatct
    29401 cgcgttcgac cctggcatcg ccaagccgga gcgtgacgct gtgcccgccc tcccccgatc
    29461 caaagacatg gcgggcgccg gtgtggtcga tgaccacgag gcgacccttg cggatgaagc
    29521 gcttcaacag atgaggtagg aacaacatgg atccattgcg cgccgaaaag caggcgaacg
    29581 tcaatggtgt ccgcaaacaa taatgtcagc agtcacttag cggacaaggc gccccagtgc
    29641 ctttggtcca gcgggcggcg gcgatccatc cagaccggca acgtatcccg ctggcaggcc
    29701 cgcggccttt gccgccgcca cgacgccttc aagatacccg ggtttgggaa ggccgggttg
    29761 cgcactgcgg ccaatgtaga ccagcgcctg ccgcgggcca gcggatgtca gcacaggctg
    29821 cacgaccttg gtgtaaaggc cctgcgccac gccctcgtag cggtcgaggg cctggacatc
    29881 cctgagcgcc aggtcccaca ggagtccatg gaccgtcccg cgtgcatcgc gcaccacagt
    29941 cgcatagcca tcgacattga tcaggaagcg atggcgcggc aggcgggcaa gaccgatggc
    30001 ccgcgatgcc gggcaacggt ccgccatcgc cgtcacatcc atgttcgagc cataggcgaa
    30061 gtagagcggc acggcgccgt tcccgctcgg cgtcaggcgg ccttgctggc cgcgccggac
    30121 ggcgcaggct tgccttcctc ggcaccgact ttcaggccga ggtagatcag caccggcgat
    30181 gcgatgaaga tcgacgaata agtgccgacc accacgccga acaacatggc ccaggagaag
    30241 gacgagatca cgttgccgcc gaaaatcacc agcgcgacga gcgccagcat ggaggtgaac
    30301 gatacgagga aggtgcgcgc caacgtctgg ttcatggcca gatcgagcaa ctcctcgatc
    30361 ggcatcttct tatagcggcg cagcaattcc cggatgcggt caaacaccac gacggtatcg
    30421 ttcagcgaat agcccacaat ggtcaggatc gccgcgatcg atgtcatgtt gaactcgatc
    30481 tgcgtcgccg cgaagaaccc gatggtcatc accagatcgt gcaaggtggc gatgaccgcg
    30541 ccgacggcga actgccactc gaaacggaac caaagataga tcaacacgcc gaacacggag
    30601 acgacgactc ccagcgtgcc ggcctgcacc aattcgccgg aaacgcgcgg tcccacgact
    30661 tccacacggc gaaactcgaa atcgttgatg aaggcggcgc gcgccttctc caccaccacc
    30721 tgctggccct tgtcgccgcc aggctgcagc acgaaacgca tcgaaacctc ggcgttcgtg
    30781 gagaattcct gcacctcgac ctcgccgagg ccgagcttct ccgccgtggt tcgcacctgg
    30841 tggatatcga ccttgccgga cttggcgcgc atctcgatca gcgttccgcc ggtgaagtcg
    30901 atgccgaaat tcatgctgac cgagaagaac agcgcgatag cgatcaccga gatcagcgcc
    30961 gagaacggaa agctgatgcg cctgaagcgc atgaacggga atttggtgcc gtccgggatg
    31021 atgcggagct ttttcatgat ctcagctcct caaaacggga tctttgtcgg cttggtatac
    31081 caataccaga gcgcgatcat catgcgggtc atggtcacgg ccgtcacgac cgttgtgatg
    31141 atgccgacga tcatggtcac ggcgaagccg cgaaccggac cggaaccgag gaaatagagg
    31201 atcgcggcgg cgatgaacat cgtcacgttg gagtcgatga tcgtcgcgaa ggcccggttg
    31261 aagcctgctt ccagcgacga cacgatggtg cgccccaggt gcgactcttc gcggatgcgt
    31321 tcgtagatca gcacgttggc atccacggcc gtgccgatgg tcagaacgat gccggcgatg
    31381 cccggcagtg tcagtgttgc gccaagcttg gtcatgatcg cgaagatcag caggatatgg
    31441 atggccagcg cgatgctggc gaagatgccg aacagcccat aggtcgccag catgaagagg
    31501 aacaccaggc acgccgccac gtacgacgcc ttcttgcctg cgtcgatcga gtcctgtccg
    31561 aggcctggac cgactgtgcg ctcctcgacg atggtgaagg ccgcaggcag cgcgccggag
    31621 cgcagcagga tcgccagccg gttggcagaa tcgaccgtga acgagccgga aatctgcccc
    31681 tgcccgcccg tgatcggcga acggatgacc ggcgcggaga tcaccttgcc gtcgaggatg
    31741 atggcgaacg gcttgccgac attttccgtg gtcacctggc cgaaccggcg cgcgccattg
    31801 atgttgaagc ggaaattgac gatcggctcg ctggacctct ggtcgaatcc cggttcggcc
    31861 gacgtgaggt cggcgccatc gaccatgacc cggcgttcga cagtaacggt accgcgcccc
    31921 ggttcgtcgg agggcagcgt ctctgtgtcc gcgggattgg cacccggctc ggccaccagg
    31981 cggaactcaa gcttggccac ctttccgagg atttcgcgca gttccgtcgg gtcctggagg
    32041 cccgggacct ggatcagaac gcggtcgatg ccctgacgct ggatggagat ttccttggtc
    32101 ccctccggat cgacgcggcg ccggaacaca tcgagcgact gatcgacggc gcggcgcgtg
    32161 cggtcattga tgccgggttc ggtgaaggcg aaggtgaaca gttccccctg ctcgaccact
    32221 tcgagattgc gaacgcctgc cccgaggcgc gcggcatcga tcggctgcgc cagttccttg
    32281 agctttggcc cgactttggc ccggtcctcc gcattcggaa cacgcacggc cgcgccgcgc
    32341 ggctgggccg ggacgccacc tgtcggtgca acgcgcgcat cgcggaaaat gccgcggatg
    32401 tcggcctgaa gctgctggac catctggcgg atcagcgccg tttggtccac ctccagcacg
    32461 acatgcgtgc cgccctgcaa atccagtccg agggtaatgc ccttgtgcgg gatcagccac
    32521 ttcggcgccc aggtcggcag cgacttttcc catgccaact gctgcgccgg tgaaaaggcg
    32581 cttggcaggg aatacaggat gctcgccaac gccagcgcga cgactgcaag agttttcagc
    32641 ttcgaaaagc gcagcatatt ccgtaacttg ttagttgttc ttcagaactg ccatgcgtca
    32701 gttcgcaacg ggctcggcct tgttgcgaac gtccgcgatc atgcctttca caacccgcac
    32761 gcgcacgttg tcggcgattt ccgcctcgac ttcggtatcg tccaccacct tggtgacctt
    32821 ggcgatcatg cccgagctca tgacaatggt atcgccacgg cgaacgttat tgatcatctc
    32881 ctgatgcgcc ttagcacggc gctgctgcgg ccgtatcaga aggaagtaca tgatgatcag
    32941 gatcagcacg aaaggtgcga actgggcgac catgtcgccc gcccccggag cgccggcggc
    33001 ctgggcaaag gctggggtaa tcacgaagga acctcactct gatggatcac aaggcgcgat
    33061 ggcgcgcccg gttaaaagtt gcgcggacta tagtgtcgaa cgcgcataag gcaacgagtt
    33121 tcggcctgan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    33181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ggtccatctt
    33241 gagcgccagc tcgatgttgt gcgtggcgac cagcgcagcc aggcccgact gcttcacaat
    33301 ccccgacagc aggccgaaga cgtgctccga ggtgtgaggg tcgagattgc cggtcggttc
    33361 gtcggcaagc agcagccgcg gggcattggc gacagcccgg gcgatggcaa cgcgctgttg
    33421 ctcgccgccc gaaagcttgg ccggctggtg tgtcaggcga tgcccgagtc ccaggtaatt
    33481 caggatttcc gccgcccgct tgccggcttc cggcttgcct aacccggcga tcagctgcgg
    33541 catcatcaca ttctcgatcg ccgtcaattc gggcaggaga tggtggaact gatagacaaa
    33601 gcctatctcc tcgcggcgga ttcgcgtgag ttcggcgtcg gaaagcccgg tgcaacggcg
    33661 atcggagatc cgcacctcgc ctgatgtcgg cttttccaac aggccggcga tgtgcagcag
    33721 cgtcgacttg ccggtgcccg atggcgcgac cagggccacc aattcgccgg gagcaatggt
    33781 caggtccacg cccttgagaa tcggcagttc ctcgacgcct tgccggtagg tgcgcaccac
    33841 gcccgcgaga atgagggtcg acatgctatt ccgccctcaa ggcttcgacc ggatcgagcc
    33901 gtgctgcctt ccacgccggg tatatcgtcg cgaccaggga caggaccagc gacatgccga
    33961 cgatcagcga gacttcgcgc gggtcgacca cggccggcag gcggctgagg aagtaaagct
    34021 ccggcggaaa cagtggcctg ctgaacagcc aggagaagaa cttgcgcagg gtctcggcgt
    34081 tttccgcgac gatcagcccg cccgcgagcc caagcagcgt gccggtaacg ccgatggcgg
    34141 ccccggtgat gatgaagatt cgcaggatcg ctccgcgcgt cgcccccatg gtgcgcagga
    34201 tcgcgatgcc acgcgtcttg tccttcacca gcatggtcag gccggagatg atgttgaagg
    34261 cggcgaccag cacgatcatc gtcaggatca ggaacatcac gttccgttcc acttcgaggg
    34321 cgccaaagaa tgtgttgttg cggaaacgcc agtccgtcag cgccacgggc gccaaggcgg
    34381 cctcctgcaa tgcctgcgtc agcgcatcga ccttgtccgg gtgctcgatg aacagttcga
    34441 gcacggatgc ctgcccttcc atgtcgaagt agagctgtgc ctcgccgaac ggcatgaaca
    34501 cgaagccgga atcgaattcc gacatgccga cctcgaaaat cgccgcgacc tcataggcct
    34561 tctggcgcgg cgcgctgccg aacggcgtgg aaggcccctt gggtgaaagc agccgcacac
    34621 ggtctccgat gccgacattg agatagccag ccatgcgctt gccgatggca atgccctgcc
    34681 cttcgtcgaa tccgtcgagc gttccttcgc ggatgttgtt ggcgatggca gcgaagcgct
    34741 tgatgtcgcc ctcgcgcagg ccacgcacca tcgcgccggt ggaggcggtg ccggacgcgg
    34801 ccaacacctg cccttcgacg agcggggccg cgagcttgac gcccttgagc gcgttcatcc
    34861 gatccgccat ggccgcgtag tccgtgagcg gctggcccag cggttgcacg aagatatggc
    34921 cgttaacgcc gagaattttc ccgatcagct ccttgcggaa cccgcccatg acgctcatca
    34981 cgacgatcag cgtcatcacg cccagcatga tgccaaggaa cgaaaagatg gcgatgaccg
    35041 agatgaaccc ctcgcgccgg cgggcgcgca ggtagcgcca ggccagcatc cattcgaacg
    35101 gggcaagcgg cccgccgggc gtgccgattc ggcccggggt gggaaaagcc gcctgtgtca
    35161 cgcggtaaac cgggcaacgg cgtcgccggg gctgagcaat tcgcgatctc cgccgacgcg
    35221 gcgcttgagt tccaccttgc cttctgccag ccccttcggt ccgaccatga gctgccacgg
    35281 cacgccgatg agatcggcgg tggcgaactt cgcgccaggc cggtcttcgc ggtcatcata
    35341 gagcacatcg acgcccttgg ccgtaagctg gcgatacagc gtctcgcaag ccgcgtcgca
    35401 actgctgtcg ccttgcctga ggttcagcag cgccaccttg aagggagcga tcgcttcggg
    35461 ccagatgatg ccggcatcgt catgcgacgc ctcgatggcc gcagccgcaa cgcgcgaggg
    35521 accgataccg taggacccca tgtgaaccgt tgtttccttg ccgtccgggc cattcaccac
    35581 ggccatcatc gggtcggaat acttcgtgcc gaaatagaag atatgaccaa cctcgatgcc
    35641 gcgcgccgaa acgcggctcg cggccggaat ggcctcgtag gccgccacgt cgtgcatctc
    35701 cgaggtcgcg gcgtagagcg aggtccactt gtcgacgatg ccctggatgc cggcgatgtt
    35761 gtcgaaatcc gtgtcgacgc ccggcacggc aaagtcgaga tagtccttgt ggcagaacac
    35821 ctcgctctcg ccggtcgaag ccagcacgat gaactcatgg ctgaggtttc cgccgatggg
    35881 gccggtatcg gcacgcatcg gaatcgactt caggccaagg cgatcaaagg tgcgcagata
    35941 ggccacgaac atcttgttgt aggcgtgccg cgccccggcc tgatcgagat cgaacgaata
    36001 tgcgtccttc atcaggaact cgcgcgagcg catcacgccg aaacggggac gcacctcgtc
    36061 gcggaacttc cactggatgt ggtagaggtt caacggcagg tccttatagg acttcacata
    36121 ggcccggaag atttcggtga tcatctcttc gttcgtcggg ccaaacagca tctcgcgctc
    36181 gtggcgatcc ttgatgcgca gcatttcctt gccgtaggca tcgtagcgac cgctttcccg
    36241 ccacaggtca gccgactgga tcgtcggcat gagcagttcg atggcgccgg aacggttctg
    36301 ttcctcccga acgatcgcat tgaccttgtt cagaaccctg agcccgagcg gcagccaggc
    36361 atagatgccg gccgattcct ggcggatcat cccggcgcgc agcatcaggc ggtgcgaaac
    36421 gatctccgct tccttgggtg tatcgcggag cacgggcaga aagtagcggg tcagacgcat
    36481 ggggagtcca ggtgcaaaac gattcagggc aagtcggcgg cagagctaac aagcggatca
    36541 cgcggaaaaa cacaagcagg acgccgggcg ttgcaaaaaa agctcctgaa gctgaaaaat
    36601 accgcatgac acgggaatgt gatgcggcta agaaagcggc gctgatcggg ccaaaagtag
    36661 gctagccccg gcttaaatgg ggcatcctgc ttggcaacgc ccaagtcttc ggaggaaaga
    36721 catcagcccc gatgggggag aatggacgcc agtccacagg gtcggggcca cggatcggat
    36781 acctgctcga gcaaggcttg cgccttgctc ttattttttc tgccctcatc gacatcgcgc
    36841 tcttgcacgc tggcggccct cgttcttgag gcgcgcgcca gcggtcggca tcaaagcacc
    36901 gatgtgaatg aggccgggaa ccagtcatag gccgcgccac tcttcgccac gaatcccgtc
    36961 gacgggaagg ccgcgtggta gaaactgatc tgcatgcggt cggcggacgc catgtcgagc
    37021 agcttcttgc gcgatgtcac cgccagcggc ttgtccgcat cgaacatcag atgccactcg
    37081 ggattgcggg cgaacaggcg cggatcattg caggcatccc cgaccaccag caacgatttc
    37141 gtgccggacg agacgacgaa ggacgtatga cccatcgtgt gtccgtccga ctgaatagcg
    37201 gtgatgccgg gcgcaacctc cttgccccac tcgaactgcc gcacatcctt ggcaaccggc
    37261 gcgaacaccc ggcgcgtcac cccgaaggct ggccgggccg cttccggagc attgttcatc
    37321 cgcgcatcgt ccatccagaa cgcccactcc ttggcaggaa cgacgagttc cgcattcggg
    37381 tagactgcgg ccccctcctt cgcgcgcagc ccattgatgt gatccgggtg aaaatggctg
    37441 accagcaccg tgtcgatctg cctgggatcg atgccagccg ccgccatgtt cgcggcgagc
    37501 tgaccggtcg ttggggcgcc gttgttgttg aatccggagt cgatcaggat cagcttcgac
    37561 cccgtgttga ccgtcatcac ggtgaagtgg ttcggcgtca cgtcggtcgc catgaactgt
    37621 gccgccatga ccttctgcac ctcttccagc ggcgtattcg ggatgtaggc ctgctggacc
    37681 tggcggggca cgataccgtc gtgcagtgcc gtgatctcga aatcgccgat cttgaaccgg
    37741 aagaagccgg gagcctgctt gccgaccggt ggagcgcccg ccagagcggg aaggcccgac
    37801 atcggaagca ttgcggcagt tgtcgcggca gcggaagcgc cgagaacggc gcgacgggtc
    37861 atcatcatgg catccctccc agagcgccgg gcagcgttcg cccgcgcctc gccccaagct
    37921 atcccgcttc cagcttgcgg caagccgcgt cacgcagccg tgatgatgcg aaatcgcaag
    37981 atctcgactg cgcaacgcca caaaaaaaac ggcggggaaa ccccgccgtc acgaacgcgt
    38041 ccgagggccg tgttcagcca acgatctcat tgcccgcgaa gaactgggcg atctcgatcg
    38101 cagcgttctc gacgctgtcg gagccatgtg ccgagttctc gcccatggag cgggcgaaca
    38161 gcttgcggat cgtgccttcg gcggcattgg ccgggttcgt cgcgcccatg acatcgcggt
    38221 acttctgcac cgcgttctca ccttcaagca cctgcaccac caccggcgca gacgtcatga
    38281 actcgacaag ttcgccgaag aaggggcgct ccttgtgcac ggcatagaac gtttcggcct
    38341 gcttgcgggt catgtggatg cgcttctgag cgacgatccg cagacccgac ttctcgatca
    38401 ccgcgttgat cgcgccagtg aggttccgct ccgtggcgtc cggcttgatg atggagaagg
    38461 tgcgttgcgt ggccatgtcg atgtttccag tgttccagat agggattgca ggattgcggc
    38521 gtcataattc acgcgcgtca aaatgcaacc atcgcaggag cgtctcttgc cgatcttcgg
    38581 gtcttggcta aaaggcgcgt catgttgcgc atgtccggac tgacttaccg ccttggcgac
    38641 cgtctcctgt ttgaccaggc ctcggcgaca gtgcccagcg gcgcgcgggc cggcttcgtc
    38701 ggacgcaacg gctcgggcaa gacaacgctt tttcggcttg ttggcgggga catcggcgcc
    38761 gaggatggcg acatcgacct tccgcgcggc gtgcgtatgg gacgtgtcga gcaggaagca
    38821 ccgggcggac cggagtctct gctcgacttc gtgctcgcag cggacaccga acggctctcc
    38881 ttgctcaacg aagctgaaac cgcgacggat ccggcgcgta ttgccgagat ccagctgcgc
    38941 ctggccgaca tccatgcgca ttcagcgccg gcgcgggccg ccgccatcct gcatggattg
    39001 gggtttgatg cggaggccca ggccaggccc tgctccgcct tctccggcgg ctggcgcatg
    39061 cgcgtcgcgc ttgccgccgt gctgttcacc gagcccgacc tgctgctgct cgacgaaccg
    39121 acgaactatc tcgatctcga aggcgcgatc tggctgcagg aacatctggc gcgctaccca
    39181 cacaccgcca tcgtcatcag ccacgaccgg gattttctcg atgccgtgac gaacttcacg
    39241 ctgcatctcg accgcgggaa gctgacgctc taccgcggcg gctactcatc cttcgaacgc
    39301 cagcgggccg aagcgatcgt gctggccgaa aaggccgccg ccgctgtcgc tgcggaaaag
    39361 gccaggctcc aggccttcat cgatcggttc aaggcgaaag cctccaaggc gagccaggcc
    39421 cagtcgcgcg tgaaacgcct ccagaagctc ggggaggcac cggcgaccgc gcgcgaagaa
    39481 ggcgccgaac tcacgttccc gaaccccgag aagccccagt cgcctcccct cgtcgccttc
    39541 gaaggcgtca gcgccggcta cggcgaacac ctcgtcttgc ggcgcctgaa cctgtcgatc
    39601 accgacgatg accgtatcgg cctcattggc gcaaatggca acggcaagtc gaccttcgtc
    39661 aagttgatcg cggatcggct cgcgccgctt tcgggaaaca tcacgcgggc gccaaaattg
    39721 aacgttgcct acttcgccca gcaccagctc gatgaactga cggcggaagc aacgccctat
    39781 ctcttggtca agcgccgcat gccggaggca cccgaggcga aagtcaggtc ccgcaccgcg
    39841 caacttggct tttcatcggc caagtcggac accccgatcg ccagtctctc gggcggcgaa
    39901 aaggcgcgcc tccttctcgg tcttgccacc tttgacggtc cgcacctcgt catcctggac
    39961 gaaccgacga accacctcga catacccatg cgcgacgccc tagtccgggc tctggcggac
    40021 tacaagggcg cggcgatcat cgtttcgcat gaccgccacc tgatcgacgc ctgctgcgac
    40081 cggctgtggc tcgtcgccga tcagagtgtg cagccgttcg atggtgatct cgacgcctac
    40141 gcccggctgg tcatgagcgc acgggcgaaa tcgcccgacg tgcgctcgct tgacctgccc
    40201 acccccgccg cgtcagacga gcgtcgcgcg cgctcggaac gacgcctcgc acttggcccg
    40261 cttcgccgga agatcgctga tctcgaagcg caaatggcgc gcctctcaga cctggccgcc
    40321 aaggtggacg tggcactggc ggcgcccgac gccttcatca agaacccaga aaaggcagcg
    40381 caactggcaa aggatcgtgc tgccctggca gaacggctcg cgaccgtgga agaagcctgg
    40441 ctcgaagcaa gcgcggacct ggaggcggcg gagggagcct gaaacaaatg cccccgccga
    40501 agcgggggca cggccttcat cccggtatcg caggcctcac gaggtgatgc gcagcttcac
    40561 aacttggccg gcgatgatct tcatcacgtt cttcagctcg gccgcgtcga tgcggtagaa
    40621 ccgcgcaaca ccattcttgt tcgggcccgc gcagttattg agcaactgga ccgcctgcgg
    40681 attggtgaag ttcaccgcta tgacgaacag atcgaccttc ttttcgttct tcagatagtt
    40741 gcaccactgg gtcgtgcgct ggtcgagcgc cgcttgatcc ggagaaccgg tgcagcccgg
    40801 gttgacgtag cccttgcacc cccagtagcc gggatagtcg atggcctgcg aattctcgcc
    40861 atcgctgatg aggatcatca tcttggtctc ctcgcccttc cacttcacgg gcggcttgga
    40921 cagaccgaag aagcttggcc agtcgccatc aggtgaaagg gtgcgcatgc cccagcgcag
    40981 gccgacgtcg gtatgcgtgc cgcccgagac cggcgtcatg cgatccagag tctcgattat
    41041 ctgcctgcgg ttgccggaaa ggccaagcat cgggatcggg cagttcaggt tcggaccgat
    41101 ggtcggatca aacttggtcc agtccgtacg cggcacccac gggcaccacg cgcctgtcca
    41161 cttgtgctcg ttggggtcgc cgacgcaggc ccctatgttc tgggtgcgct cggtaccggc
    41221 gagaacgcag gcgccgctct ggctgttgtt ggcgcatacg atcgggttgg cgctgtagac
    41281 gttgcgacgc ccttccttga tgtcctgctc gcaggccggg taggtcttgg atggaatgct
    41341 gccgtcgcac ctcaactcca tgaccgtttc tgtccagcac cgatcttcca ccgtgcggta
    41401 gcaggtgttg gccttctgca ccggcggctg aagcacgtca gcatccttga accggtcgcc
    41461 gaaaaaggcg gcaggctgca ccggcgcatc ggtcgtcttc agatcatcga ggaagccctg
    41521 catgccgccg gaagaggagg acgacgaaga ggatgatgat gaggacgatg aggatgatga
    41581 ggaggaagac gacgacgaag aagaagacga agaagaggaa gaccaggaag acgatgagga
    41641 cgaggacgaa ctaccgctgg aggaaggtgg cggcggcggc ggcggacatt cgtaagcctc
    41701 ctgcttatac gtgcagcgcc gatacggtat cggcatcatc ttgccttgct gtatcgtgac
    41761 cgtgtcgaga ctgttgtttg gaacgttcag cgcattccag atcatgccgc tggcatccga
    41821 cggaccaggc gcctcacccg atcccgatat gcagccgcgc cagccgaccg gcgcaaacag
    41881 cgcggaattt acgtttaccc gcccgccacg cagcatcggc gaggccaccg gccttttgct
    41941 gccatagtcg ggaatcggcg ttacggccgc gtcaccggcg acatccgggg ctcgcggcgc
    42001 ttcgctcagg acattgacgg tggcagccca aggaacaacg gacaggcgga gccagtcttt
    42061 ggagccggac tgctcggcgg cattgaacag ttgatgagtg aacagcttgg cggaatcgcg
    42121 caactgcgtg aagcgggtct gagcgcgacc gtccttcatg aacatcgacg ccgtgttgtc
    42181 gagcacaagg gccacttccg tcggcagggg taccgagaag ttcgctgtcg cctggacctc
    42241 gacgtccgag aacgcctcac cgatgatgcc cgcgaacaac gtattcagct tcgccttgac
    42301 cgtcaccttc gagctgccat ccgccagcca ggtgatcgtt gcattcggcc gtgccacgcc
    42361 atcgaagacc gacgcctcgg atatggctgc atcgaagatc ctcttgccgg cgatctcgcc
    42421 agcggcacgg gcctgagccc gctgcatcgt cgtcagagaa tccgtctcgg tacgcttcgc
    42481 cgcaagagcg gcggcatccg ctgccttctg caactcggtt ccgccaaggc tgcgctggtg
    42541 cagctcgacc gatccggcga tcagcgtgag gatcgccatc gacgcgccgg cgaacatgat
    42601 ggcaacgccg ccgcgcctgt cttcagcgaa ccgcgaggca attgagccaa atctcttgaa
    42661 cccggacatc ggcttcggcg ccatgattcc ctccaacgca ccgcccgcgc actcagcagg
    42721 cgtattgagc cacattcgag gaaaaccaat gccgggacaa aaataacatg cctttaacgt
    42781 gatcgttgaa actcgcgcgc atcggcaaaa atctcctaaa atttttcgcg tgttcgccga
    42841 aaggttgcga agtcagaaaa gcctgcagcc cgttattccc gatttgttaa cgatagcgcg
    42901 ccatcgtgcg cgcatgactt acgtcttcac gtccacgccc gagcgcgggt cggaagcgcc
    42961 gcagtccgtc catgcaaatg cggctgcgcc agtcagcctc gccatcagtc tttgcggtct
    43021 ggccgctgcc gcactcacgc tcgccttttg ccgccagtca ggcgctctgc ggatcgaagc
    43081 ggcattgctt gtctgcatgg cctacgccgg tacggtcctt gctggcgaga ccctgctcct
    43141 gcgcacgcct tgggcggcct ccgctggcct cgatttcgcc cgacgcggct tcgaccttgc
    43201 ccggatcggc accaagctcc tggggctata cggcgtgttc gcggtgctgg catgcatcta
    43261 ctggctgttt ccggagtacc gcgcagggat gtaccagccg acctgggtag ccgcgcagca
    43321 gctcctgcct tacgtgctga tcctggcggt tccctacgtc gcgtgtgtcg atcgcgcgat
    43381 gaaggcgccg gaagacgggc tttactggtt cggccgcctg ttgctgcttg accgcgaccc
    43441 ggcgcgctgg ccggccctgc gccagtttgc tctgggctgg atcgtgaagg gcttcttcac
    43501 gccgatcatg ttccacggcc ttgtacagaa caccgagacg ctgatgcgct cgtccttcga
    43561 tctgggaagc ctgacattcg cacagttcta tgcgctcgcc acggtctgcc tgttctggac
    43621 cgatgtcatg gtcgcctgcg tcggctatgt ttttgcgctc cgtctgtttg acacgcatat
    43681 tcgttccagc gaaccgaccg cgttcggctg gttcatctgc ctgatgtgct atccaccgtt
    43741 ccagactgtg ctgatggcgc agtatttcgc cttcagcgcg cagaacgccg tgcgcctgct
    43801 gtccgactgg ccgctcctcc agatggtctg gggagtggct gcgcttgcac ttcttgtgat
    43861 ctacacgctg tcaacgcttg tattcggctg ccgcttctcg aacatcaccc atcgcggcgt
    43921 gattgccgcc ggcccctacc gcttcaccaa gcacccggcc tatgtcacga agaacctgta
    43981 ctggtggctc gcgcatgcgc cgttcatccc ggtcgccggc gccggtgaag ccctgcgctt
    44041 ctgcatcctg atggtcggca tcaacgtgat ctactacctg cgttcgaaga ccgaggagcg
    44101 gcatctctcg cacgacccgg cctacgtcgc ctatgcgaca gcaatgaacg acaagagcct
    44161 gttcgctccg gttgcgcgca ttctgccctt cctgcggtat cgatcaccgg aatgcggaac
    44221 cgcgcacgat ggcccctaca agggcctggc ataggcgacg cggcagccgg atttcacaga
    44281 agcatcgggc tgtgccacaa ggtggttcct tcatgcgaac ccattgagtc ccttggcacg
    44341 ccgaaagcct cccgaagacg gattcgtttt gccctcgcgc gaggacgtgc tggccttcgt
    44401 cgcccgttca ccgggcaaag tgggcaagcg cgacatcgcc cgtcacttcg gcgtcggcgg
    44461 cggtgcgcgc gtggctttga agcgtctgct caaggacctc gaggccgacg ggctgctgtc
    44521 gcgccgggcc aagacgctgc atcgcgccgg cgaattgccg tcgatggtcg tcgcgcacat
    44581 cgcaggcaag gatcgcgacg gagagctgtt tgccgccccg gtcgagtggg acgaagagca
    44641 aaacggccgc atcccccgca tccacgtgcc gattctcagg cgccagaaac atgccgggcc
    44701 ggcccctgcg gtcggagacc acgtcttgct gcgggtcgaa cgggatcgtt cggaagagga
    44761 tcaatacacc ggtcgcatcg tcaaggtcct gacgcagcag aaggaccgga cgctgggcat
    44821 tttccgcgag gacaagtcgc gcggcggcgg ccgtctcctg ccgatcgaca agaaatcacg
    44881 cggcctggaa atggtaatcc ggcccggtga cgaacaggac gccgaggacg gcgatctcat
    44941 tgcggtcgat gtcttgcgtg acaatcgcct tggcttgcgt tcggcgcgcg tgcgcgacgt
    45001 gctggggagc gtgaaatccg aaaaggcggc gagcctcatc gccatccacc agcacgatat
    45061 cccgcacgtc tttcccgagg cgacattgca ggaggctgcg gcggcgcgcg agccgaccct
    45121 tgcgggtcgc gaggactggc gggcaatccc cttgctgacc atcgacccgg cggacgccaa
    45181 ggaccatgac gatgcggtcc atgccgaggc ggacgatccg cagaccaatc ccggtggttt
    45241 catcctgcgc atcgccatcg ccgatgttgg cgcctatgtg cggcccggct ccatgctgga
    45301 ccgggaagcg ctcaatcgcg gcaattctgt ctattttcct gaccgtgtcg tgccgatgct
    45361 gcccgagcgc atctcgaacg acctatgttc gctcaagccc ggtgtggcgc gcgccgcgat
    45421 ggcgcttcgg gtcgtcatcg atgcgcaagg ccgcaagaag agccatggct tccatcgcgt
    45481 gctgatgcgt tcggccgcca agctcgccta tccccaggcg caggccgcca tcgacggccg
    45541 tccggatgat gtcacaggtc cgctcctcga aaccgtgctc aagccgctgt gggacgccta
    45601 tgccgccctg aaacgggcaa gggccaagcg tgcaccgctc gatctcgacc tgcccgagcg
    45661 aaagcttgtg ctcaatcccg atggcaccct gaaggacgtg atcgttccgg agcgcctcga
    45721 tgcgcaccgg ctgatcgagg agttcatgat cctgtccaac gtctgcgcgg ccgagacgct
    45781 ggagaaggcc aacacgctgc tgctgtaccg cgtgcacgac gagcccagtc tcgaaaagat
    45841 ggaatcgcta cgcgaattcc tcgaaaccat cggcatcgac ctgccgaagt cgggcgcgct
    45901 caagccggag ctgttcaacc gcatcctcac ccgtgtccgg ggaacgccaa acgagattct
    45961 caccaatgag gttatcctgc gctcgcaggc gcaggccgag tattcgcctg agaactatgg
    46021 gcatttcggc cttgcattgc gacgttatgc ccatttcacc tcgccgatcc gccgctatgc
    46081 cgacctgatc gttcatcgcg ccctgatccg ggcgctcgac ctgggaaccg atggtctgcc
    46141 cgagacgacg ctggaccaga tgcgcgagat cggcgccact atttccgcag ccgaacggcg
    46201 cgccatggcg gcggaacgcg ataccgtgga ccggcttatc accttccacc tcgccgacaa
    46261 ggtgggaacg agcttctccg gccggattgg cggcgtcacc cgttccggat tgttcgtgcg
    46321 gctcgacgga atcggcgcgg atgggttcgt gccagcggca acgctcgggg cggacttctt
    46381 tcgatttgac gagaaagcgc atgcgcttgt cggcagccgt accggagaaa cctatcgcct
    46441 gggtgatccg gtcaaggtac ggcttgtcga ggcgcaaccc gtggctggcg ccctgcggtt
    46501 cgaaatcctg tcgcagggac gctatacaac cccggcccgg gggcgttctt ccccgggcaa
    46561 aggccgacac ttcgcgcgcg gcgcgcggca gggccgacga gatcagagcg gatagaacca
    46621 tggcgattga gtaccgggtc ggagccaatt ctggagttga cgaggcgcgc gactgggcga
    46681 gcggcctgag gcgcggtctt tcctgccgct gcccccggtg cggcgaaggc cgcatgtttg
    46741 ggcgcttcct gaaggtgaag cccgcctgcg agacctgcgg actcgaactg tccggccacc
    46801 gcgccgacga catgccgccc tatgtcgtga tgatgattgt cggacacatc ctcgttcccc
    46861 tcgtccttct ggccgacaat gccgaggtgg attggccgat gtggctgcac atggcgctct
    46921 ggccgacgct gacactcgtc ctctgcctgg ctctgcttca acccgtcaag ggagccttga
    46981 tcgggtatca gtgggcgctc aagatgcacg gattcgatcc ggaagccgcc acgcgcgatc
    47041 cggcgcttcc agccccccta ccggatgccc ccgcccgatg accaatcctc atgtcgagac
    47101 tatcgaacgc aaatacatga aggggcagca caaggccgtg cgcccgcgtg atgccgcaac
    47161 aatcatcctg atcgaccggc gcggcaggga gccgcgcgtg ctcatgggct tgcggaacga
    47221 agccgccaag ttcatgcccg gcgtcttcgt cttccccggc ggcagggtcg agatcgagga
    47281 tggcctcgtt ccccatgccg gagcgctatc cgcgcgcgat gtcgaggcat tgcacaagat
    47341 gtctggccgg ctttcggagc gccggatccg cgccttcgct ctcgccgcga tccgcgagac
    47401 gttcgaggaa accggcctgc gtatcggcgt tcctggcggc gccgcgctgg agaggccggg
    47461 agcctggcgc aagggaacct gggcagagtt caccgagacc ggactcatgc cgaccatcga
    47521 cgatctgcat ttcctctccc gcgccattac gcctcccggg cgcaatcgcc gtttcgacac
    47581 acggttcttc gtgcgcgacg tatcggcact cgccgaacag gtggtggacc gcacgacccc
    47641 ggacagcgaa ctcgtcgagc tgaagtgggt gccgttcgcc gagacctcgg acctgaaaac
    47701 ggtcgagatc accaagatca tgcttgagga actgcaaagg caggcagagc aggagtttgc
    47761 accctcactg ccccggccga tgtaccgcgc gctgaacgga aagttccagc gtattcagct
    47821 ctgaaggcac tatgagcgaa actggccgcg ccctcatcgc agcgattgcg ctggtcagtc
    47881 tggctggcct tggcctgagc gcgatgatcc cgctgctgtc gctcgaactg gaacgaatgg
    47941 gcgtttccag cgccatgaat ggcctgaacg ccgcggtcgg cggcatcgcc aacatcctga
    48001 ttgctccggt gacgccgttg cttgccggcc ggatcggcgt tcggcggatg atcgccggcc
    48061 tgatcctgat cgacgcggca gcgatcctgc tgttcccgct gcttccggac ttctgggcct
    48121 ggtgcctcat tcgttttgtc tttggtgctg cgctcggcgc catgttcatc ctgtcggagt
    48181 actggatcac cgcactcgcg ccaccggacc gccgtgggct ggtgatgggc atctacgcct
    48241 cgttcctcgc gatcgggttc gccgccggac cggtggtcct ggcgcgcgcc ggcacctccg
    48301 gctgggcgcc ctatctcgtc accagcgccc tgttcctcgc cgcgctggcg cccttggcct
    48361 tggcgccaaa atccccg
//
LOCUS       DAOMXN010000005        45814 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_578_length_45814_cov_12.679342, whole genome shotgun sequence.
ACCESSION   DAOMXN010000005 DAOMXN010000000
VERSION     DAOMXN010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 45814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45814
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_578_length_45814_cov_12.679342"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="PK812_01155"
     CDS             <1..341
                     /locus_tag="PK812_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68188.1"
                     /translation="AHLCCFADLAELVRQGEHDRHGIYMMVPGLLRQMRTGAIAGLAA
                     PRPQLACMGAKDPLTPPSAIAVAVADVRASYAARNALDRLTITIDAEAGHCETPEMRT
                     AVLDFLTRQL"
     gene            577..2277
                     /locus_tag="PK812_01160"
     CDS             577..2277
                     /locus_tag="PK812_01160"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HRJ68189.1"
                     /translation="MTFDPALRDAAFSANAWPFEEARKLVARVEKTGQKQVLFETGYG
                     PSGLPHIGTFGEVARTTMVRRAFELLTGGTISTRLIAFSDDMDGLRKVPDNIPNKDLI
                     AAHLGKPLTEVPDPFGTHESFGAHNNARLRAFLDAFGFEYEFLSATQCYASGRFDRTL
                     LFMLQRVEAIMEIMLPSLREERAASYSPFLPIHPETRVVMQVAIEKVDARAGTIAWRD
                     PDTGKAFETPVTGGHCKLQWKPDWAMRWVALGVDYEMAGKDLIDSVKLSSAIARALDS
                     TPPDGFNYELFLDEKGQKISKSKGNGLTIDEWLTYASPESLSLFMYQKPREAKKLYFD
                     VIPRCVDEYLSFLDAYPRQDEKNRLGNPVWHIHSGAPPAPERISNEANGRGSGISFAL
                     LLNLVAVANAETKDVLWGFLKRYAPSASPATHPQLDALVGYAIRYYTDFVKPKKVYRA
                     ADADEIAALARLAGRLRALPPEATSEAIQEAVYDVARPIPRFQDMKAKGATPERPGVS
                     NLWFNTLYQILLGEERGPRFGSFAAIYGLSETATLIDRALAGELIREHEAFVAKRAEG
                     "
     gene            complement(2306..2935)
                     /locus_tag="PK812_01165"
     CDS             complement(2306..2935)
                     /locus_tag="PK812_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRJ68190.1"
                     /translation="MLKHEQIWHAIDALAARHGLSASGLARRAGLDPTTFNRSKRLTP
                     DGRQRWPSTESVAKILMATQESLDTFVSLISVQDPAAGLRMPVISLSEAASPAAFSEL
                     GEPAGGTWDEIALPGFQGRKSFAVEIDSPSASTLYGEGTVLVASCDAQVRRGDRVLLR
                     LKDGGMVLGEVRRETTNGIDLARTEASGPSQFFPRDDIVFVARIFWASQ"
     gene            3087..>3343
                     /locus_tag="PK812_01170"
     CDS             3087..>3343
                     /locus_tag="PK812_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HRJ68191.1"
                     /translation="MPLVFKIVDRHAWQRAEALGSFDGAPVDLRDGYIHLSAAHQVRE
                     TAAKHFRGQDDLLLVAYTSEVLGKDLRWEASRGGDLFPHLYG"
     assembly_gap    3344..3443
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3444..3583
                     /locus_tag="PK812_01175"
     CDS             <3444..3583
                     /locus_tag="PK812_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HRJ68192.1"
                     /translation="DLRWEASRGGDLFPHLYGSLPTAKALWAKPLRLDADREHLFPDL
                     S"
     gene            3580..4668
                     /locus_tag="PK812_01180"
     CDS             3580..4668
                     /locus_tag="PK812_01180"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HRJ68193.1"
                     /translation="MITSLLFRAARPALFVLDAETAHQASLTALKFAPVLPCAPDDPL
                     LASEALGLSFPSPVGLAAGYDKHAEIPDRLLALGFGFVEVGAVTPRPQPGNPKPRAFR
                     LAGDEAVINRYGFNSEGLEVVAARLEHRKRRGIVGVNLGSNKDTSDRAADYEILVARL
                     AHCADFLTANVSSPNTPGLRSMQNRAQFDDLLARVVAGRDAVCAGGRRTPVLVKIAPD
                     LTDADLDDVTDVALKRGIDGMIVSNTTISRPESLRETALAKETGGLSGRPLFRLSTQV
                     LAKVALRTEGRLPLIGVGGVDSGEAALMKIRAGATLVQLYTALVYHGPGLIDDIKRHL
                     ATTVRKGGHTSLAPLIGSAAKEIAAGAF"
     gene            complement(4676..4942)
                     /locus_tag="PK812_01185"
     CDS             complement(4676..4942)
                     /locus_tag="PK812_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6460 domain-containing protein"
                     /protein_id="HRJ68194.1"
                     /translation="MSDSRVQQFLGGSPVSVLIKLVLLSLLVGVILAAFGFTPWSFLH
                     YLRYLVHEIFGSGIEALYTMGGYIAAGAVLVVPLWLLIRLLARK"
     gene            complement(5043..6107)
                     /locus_tag="PK812_01190"
     CDS             complement(5043..6107)
                     /locus_tag="PK812_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ68195.1"
                     /translation="MAKQPASVEFRNVTKRYGGVVAVDDVSFTIAPATLVTLLGPSGC
                     GKTTSLRMIAGLETASDGRIIIGDKDVTNLSAADRDVSMVFQSYALFPHMSVLDNAAY
                     GPSVQGATKARAREMAMDKLTMLGLAGMEHRFPSELSGGQQQRVALARALVLEPQVLL
                     FDEPLSNLDAKLRRRVREEIRDLQLRLNLTVAYVTHDQEEALAVSDKVIVMSNARIAQ
                     VGAPHELYEEPASLFVADFIGDSNLIEAEVVSVTTGFAKVRVGEAMLDLPSSNGRTGK
                     ATLVVRPTAARIYCKKPAEPHVRGKVAKVAYLGKHMEYTVETDVGEVFLVEDVRPDPW
                     RKNEDVYVAFNPLKVKILQE"
     gene            complement(6128..>7041)
                     /locus_tag="PK812_01195"
     CDS             complement(6128..>7041)
                     /locus_tag="PK812_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRJ68196.1"
                     /translation="KVYTTITGKGDSGLQLPLPKRVEWGAYLVAIPWGLFTVAVYALI
                     LVAGFVKTVGRDYTPTLQHYITAFKIEQGQFGLVFSGSAWNSLFATMKIAAIAAPFTA
                     AIGLLTAYLLTRQRFAGQRAFEFGTLLSFAIPGTVVGVSYILAFNVPPIEITGTAMIL
                     VICFIFRNMPVGVRAGVAAMSQIDKSLDEASLTLGAGSLTTVRRVMLPLLRPAILVSL
                     VYAFVRAMTAVSAVIFLVSAEYNMATIYIVGRVDAGEHGLAIAYSSALIVIMGIVITL
                     IGLAVGERKLGRRGTGGGPATMPIPAG"
     assembly_gap    7042..7141
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7142..8436)
                     /locus_tag="PK812_01200"
     CDS             complement(<7142..8436)
                     /locus_tag="PK812_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRJ68197.1"
                     /translation="MMRRGWFWCCVALVGVAICPWYLEPPGAGTLGKIVNPAHSALAL
                     ALSSRFWLILPVLVLAGLLASLLLRSRREAALATSLTVGGIFGLVVVLGQGFLIGLKG
                     WNLDVVTTLFGAGPSQSGMGTGALVVLSAFLMLACVGLAGRGWCRGDLFVVASIGWIV
                     ALIALFVFFPVLTILSSAVEDNAGHFAPHLFAQKVTDSSIWGLGCLGGGTACGVAWNT
                     LFLAVLVGISSTLLGLAFALISARTGFALKGSLKLLAILPIITPPFVIGLALILIFGR
                     SGAVSSVLFQWFGIPPSRWIYGLPGVFIAQTLAFTPPAYLVMLGVIQGISPTLEEAAQ
                     TLRAKSWTIFLTLTWPLMRPGLANSFLLGFVESLADFGNPLVLGGNFEVLSTKIFFAV
                     VGASTDQGRAAVLSLVLLVFTLTGFAAQQLWVGKKVYTT"
     gene            complement(8467..9495)
                     /locus_tag="PK812_01205"
     CDS             complement(8467..9495)
                     /locus_tag="PK812_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRJ68198.1"
                     /translation="MRQPVIAGGMAAIVLAALATTAMAQAQVTLYCSILEEQCREGAA
                     MFEKATGTKVLMIRKSTGETYATIKAEASNPRGDIWWGGPGDAHLQAAEEGLTEEYKS
                     QKLGELQPWAVRHAEQSKYRTAGTYLGALGLGINTEVLARRGIPEPKCWADLLDPKFK
                     DEIQMADPNSSGTAYTFVATIVQVMGEDKGFEYMKALHRNVSQYTKSGIAPVNALKLG
                     ETGVGISFLHDVITQKLAGAPLKTVAPCEGTGYETGSVSLIKGGKNPEAARRFYDFAL
                     GTEAQEINNRLKIYSMPSNRNVKVPADAPDFSTMKLITYDTAKYGSSAERTRLLRKWD
                     AEVKSLPR"
     gene            complement(9560..10594)
                     /locus_tag="PK812_01210"
     CDS             complement(9560..10594)
                     /locus_tag="PK812_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRJ68199.1"
                     /translation="MFKTHVRAGALAGLALGSLAIAPAHAQGNLVLYCAVQEEWCRAV
                     STMFEKDTGIKVAMTRKSSGEFYAQLKAESANPRGDIWWGGTGDPHMQAAEEGLSQEY
                     TSPMNAQLHDWAIRQHQATKGRSIGIYTGALGFGYNEKVLKAKKLPEPKCWADLVKPE
                     YKDEVQVADPFSSGTAYTLLATIVQIQGDDKAFDWLKALHKNINQYTKSGAAPAKATS
                     LGETTIGITFMHDMIAMARENPDVKVVAPCEGTGYEIGSMSMVKGARNPDAAKKFYDW
                     ALTAAPQALGAENKQFQTPSNKATPLPKFGVDLSQTKLINYDFNKYGSSATRNALLDR
                     WAKEVKNAPK"
     gene            10770..11201
                     /locus_tag="PK812_01215"
     CDS             10770..11201
                     /locus_tag="PK812_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRJ68200.1"
                     /translation="MKVRVTWADNMMFVGETGSGHALVMDGAPEAGGKNLGVRPMEML
                     LLGLGGCTAFDVVLILKRGREPVNGCRVDVEAERAESEPKIFTKIHMIYRLSGKGLSP
                     VKVERAIQLSKEKYCSASAILGATAEITHEFTVSDERELNG"
     gene            11198..12049
                     /locus_tag="PK812_01220"
     CDS             11198..12049
                     /locus_tag="PK812_01220"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRJ68201.1"
                     /translation="MSVPSQGLVAPHVLAAVLADPGVFVLDCRSGPDGAGRAAFEQGH
                     IPGAAHTDYGADAWRVKIGGAPGMMPERAEVAGKLGRLGLEPYHRLVICPAGLSANDL
                     AAAARIAWSARMAGHRAVALLDGGFAGWVAAGLPVATGASLPRPATDYPLAPPTAQRR
                     SAAEVLAAVTKGTATLVDGRAESYYRGREKASEALAAGHIPGAINIDYVMAYDALTNR
                     LKSPAQLATVYATLPPGAVISYCNTGHTAALNWFVLAEVLGRDDVALYDGSMTDWTQD
                     MSRPVSI"
     gene            complement(12042..12968)
                     /locus_tag="PK812_01225"
     CDS             complement(12042..12968)
                     /locus_tag="PK812_01225"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRJ68202.1"
                     /translation="MTDDLRTSGHLAADRRYLWGEGLREEGEHAAAADLFRQAVDIAP
                     GWATGWFAYGRAAMEAGQTDVAREALRRCLELEPADVLGASLLLARLDGMQDGFALPE
                     AYVAGLFDAYAHRFDKHLTGTLAYRGPSVLCTALDKACFRLGRQDRFGMTLDLGCGTG
                     LMAAALQDRAGPIDGVDLSSRMLAKAGDTGLYRSLRNSDAVAALGSSHETYDLIIAAD
                     VVVYIGDLAPLLETARPRLGHHGLLALTAQRQDGKGYSLGADLRFSHSEAYLRQATQA
                     AGYHLAVLDITSTRRDAGVDVPGLVAVLTPVR"
     gene            complement(12965..14623)
                     /locus_tag="PK812_01230"
     CDS             complement(12965..14623)
                     /locus_tag="PK812_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68203.1"
                     /translation="MNFRSAGSVLLVAVVFVLVSGSMVADRLGAPSELVAKLTLMLAV
                     GGLTAAGFAARSMKPSAFFAVGRNLSATAVLALMVTTMMSLGAVRATATGVLDLAMVS
                     AACAGVLLFSATAAGPLRRLGGYTFADAIAARFGSRLLTAIAGILSFCMLGGLAVWLA
                     PATLRGVQMAGNLKPEWASLTVLTLCASVIVPGGLAGVAAAALLSSALIVGAWASPIL
                     PAMFADSSTRTHWLEHAGTAFTSGSAAGADWPLLALAAAGLFTVLNLSGTVRSQKAST
                     QVGCFAVMAVIAISFAGVFAHEVERQRVAMIESASVQTMPPIVFTDRFRGLVRICGSE
                     PTTPDDLKRICARAGHSEGLPRSAVQIDKRGGHWYATILAIPSAAGEAFALAAPLALL
                     VGLALACHAAAASLTHDLLRRFVLPPATAGGDLAVQRMAALLVLAAALYGPGYVPTVD
                     PRLVSLLLFAAACCPLPLVVLARWRRADWRAALCGLASTLLAGAAVWTGVLEGPSGVL
                     AGIACSLCAGAFAAAVFPASKAQEHVLAVIEGAAPGPLILDRSA"
     gene            14809..15489
                     /locus_tag="PK812_01235"
     CDS             14809..15489
                     /locus_tag="PK812_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008550072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRJ68204.1"
                     /translation="MAGGAEAVSVIIADDHPLFRGALRLAVDGMFSAQKIVEAASLDE
                     VLSALQKTPDVDLVLLDLRMPGMSGYSGLTHIRSQYPGVPVVIVSGASDPTTIRRCLD
                     FGASGFIPKTTDIDSMRKAIRAVLNGGSWTPADFDPASPVDKETTELVRRLGSLTPQQ
                     MRVLMMLSEGLLNKQIAYELSVSEATVKAHVSAILTKLGVESRTQAVIAAAKIGVGEG
                     KTAGESGA"
     gene            complement(15497..16528)
                     /locus_tag="PK812_01240"
     CDS             complement(15497..16528)
                     /locus_tag="PK812_01240"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HRJ68205.1"
                     /translation="MTVRKGLVEAIGHTPLIRLNRASELTGCEILGKAEFMNPGGSVK
                     DRAALAIVQDARQTGALRPGGIIVEGTAGNTGIGLALVGNALGHRTVIVIPETQSQEK
                     KDMLRFAGAELVEVPAVPYANPNNYVKVSGRLAERLNASEPNGAVWANQFDNTANRRG
                     HIATTATEIFAQTYGRVDGFICAVGTGGTLAGTGMGLKALNPKIIIGLADPPGAALYS
                     YYTSGELKASGSSITEGIGQGRITANLREAPVDVAFQIPDEESIPIVFDLLENEGLCL
                     GGSSGVNVAGAIRLAQHLGPGHTIVTVLCDSGTRYASKLYNPDFLHAKALSVPSWLER
                     RTKIDPGLI"
     gene            complement(16537..17013)
                     /locus_tag="PK812_01245"
     CDS             complement(16537..17013)
                     /locus_tag="PK812_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRJ68206.1"
                     /translation="MIEIARLDAIDRKILSLLQEDCSLSMAQISEKVGLSQTPCWKRI
                     QRLEAEGVIRGRVALVSPETLGLGLSVFVSVETGDHSGPWLENFATVVSAMPEVMSLH
                     RMAGDVDYLLHVVVSDMAAYDRFYKRLIATIPLKNVTSRFAMERIKSTTAYAVPAA"
     gene            17225..18418
                     /locus_tag="PK812_01250"
     CDS             17225..18418
                     /locus_tag="PK812_01250"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HRJ68207.1"
                     /translation="MPNERRASNALHPETRLVHEGALRSQFGETSEALFLTQGHVYES
                     AEQCEARFLGQEEGFIYARFSNPTVQMFERRVAALEGAEAARATATGMAAVTAALMGV
                     VRAGDHVVAARALFGSCRYVVEEHLPRYGVASTLIDGPDLAAWRRAVQPNTKLFFLES
                     PANPTLEVIDIAAVAALAREVGAKLVVDNVFATPLWQKPLTLGADCVVYSATKHIDGQ
                     GRCLGGVILASRQFIDDHVHTLLRQTGPAMSPFNAWVLLKALETLPLRIRRQTETAGI
                     IADWLAGQPQVRGLRYPGRQDHPQRELIARQMLGPSTLIAFDVAGGKAAAFALLNRLR
                     LIRISNNLGDAKSLIVHPATTTHQRFSPEQRAEMGVSDGMVRLSIGLEHPDDLIADLA
                     QALSA"
     gene            18470..19276
                     /locus_tag="PK812_01255"
     CDS             18470..19276
                     /locus_tag="PK812_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRJ68208.1"
                     /translation="MTETVQKTGPRVALFVTCLVDTMRPSVGFAAAKLLEDAGCTVEV
                     PRQTCCGQPAFNSGDRNTARALAEQMIAAFGTCDYIVAPSGSCAGMVRAHFPELFAGD
                     PNMAPKANAFAAKTHELVSFLTDVLGVEAVEARHEGAVTYHDSCSGLREMGVKGQPRK
                     LLATVQGLTLTEMAENETCCGFGGSFAVKYGELSDAIVSKKAENIEASGAPMLLAGDL
                     GCLLNMAGKLQRRGSAVQVRHIAEVLAGTTDEPPIGVPRSPLGDTGGGRG"
     gene            complement(19385..21136)
                     /locus_tag="PK812_01260"
     CDS             complement(19385..21136)
                     /locus_tag="PK812_01260"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRJ68209.1"
                     /translation="MAGWEALVASGALDEETYLQRLGRWIDAPVIEQAQPDPRVRLED
                     ALRQGWCRARLVDGRDVLLVSCAGPLLRVLLETTPRLRHGQVALATGDAYRSILIRHF
                     SERIALDAAESVPGALSARSGAVMRQKRVLGFCAVLSALCAALWPQEMLTLLPLVLGL
                     LFLGASLVMLSACVEGAPLATRVIETNDTRLPRYSILVPLYREAEVVEKLIAALNLID
                     YPREKLQVLLVIEEHDLEVRSRLERLLLPASFSVLVAPAGAPHTKPRALNAAMPFVTG
                     EHVVVYDAEDEPDPDQLRAAAAAFRAAPKRVACLQARLAIDNTFDSVLTRLFTIEYAA
                     LFDVVKAGNARLGLPVPLGGTSNHFRVEALRRIGLWDAWNVTEDADLGIRLAQHRLLV
                     DDLPSTTTEEAPSTVRPWLAQRTRWLKGWMQTVVVHSRAPLTAIRAMGPVNFFSAVSM
                     SAGVVVGALGAPIFYALLAQRLMRQEPFGAGGPLDRFADAVTIVLGVAGLLSTVLPAL
                     LGLHRRRLHGLIPWIALLPLYQLLVSVAAWRAFFELLKAPHRWNKTRHGLARTSRRAQ
                     KQATQAAASSSTKTALP"
     gene            21394..22248
                     /locus_tag="PK812_01265"
     CDS             21394..22248
                     /locus_tag="PK812_01265"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRJ68210.1"
                     /translation="MSVFLSRLLVPVLAFFCGFLGDERPAQAEEKVFIPGYWDLRRRA
                     EKPDVSRIRQIRFLTESDYPPFHFIGPNGMLTGFEVDLARASCEVLGVTCTIQPHRWD
                     RLLDALRGNVGDAVIAMVAITPENRRQFLLTSAYHRTPARFVVSRTAPGGALGADALK
                     GKRVAVVVGSTHEAYLRAHFPTAVVVAEPNIASVLTSVQYGGVAAAFVDGVSAAFWLN
                     GEASGNCCRFAGGAFTESRYFGEGAGIVVRRDSQHIQQALDWALQRLASDGTYATLYL
                     KYFPVGIY"
     gene            22421..24208
                     /locus_tag="PK812_01270"
     CDS             22421..24208
                     /locus_tag="PK812_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HRJ68211.1"
                     /translation="MPVYKAPVDDVSFLLNDVFHIERYGNLPGFSDLSPEVLEAIVSE
                     AAKLCEEQLQPLNRVGDQQGCKRHDDGSVTTPKGFKEAFRAYSDGGWLGISAPEQFGG
                     QGLPYTVGAIMNEFSSAANMAFSMYPGLTLGAMAAIAQHGSEEQKALFLPRMVEGKWS
                     GTMNLTEPHCGTDLGLLRTKAVPNGDGSYAITGTKIFISAGEQDITENIIHLVLARIE
                     GAPAGVKGISLFIVPKMNVNPDGSLAGRNAVVCGSIEHKMGIHGNSTCVMNYDGAKGF
                     LIGQENKGLAAMFVMMNEARLGVGIQGLAQSEAAYQNAVAYAKDRLQGRALTGPKEPA
                     KAADPIIVHPDVRRTLLTIRAFNEAARAFVLWTSLKSDVAHRSGDAKEQQAADDMLGL
                     MTPVLKGFLTDVGFDNAVRAQQMFGGHGYIEEWGMSQFVRDARIAMIYEGANGIQALD
                     LVGRKLGRDGGRGIMTFFGEVGQFLKDNSADEGMKPFVGPLQTSLGHLQQATMWFMQN
                     AMAKPDNAGAGSTDYMHLMGFVVLGYMWALQAKVAQEKLKGGANDRMQAKLAVGRFYM
                     ERMLPETGMRLARVTVGADSVMALPAEAF"
     gene            24223..24477
                     /locus_tag="PK812_01275"
     CDS             24223..24477
                     /locus_tag="PK812_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68212.1"
                     /translation="MARKTMPLPVRFNAALSEAAYAQLRALNARTGLSNNYLLTILLE
                     RLDAIADPDRLQTEFDAFISEYGAPASGVRMSKGSGGPNG"
     gene            24470..25678
                     /locus_tag="PK812_01280"
     CDS             24470..25678
                     /locus_tag="PK812_01280"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HRJ68213.1"
                     /translation="MADAFIYDHVRTPRGKGKADGSLHEVTAVNLSIQTLKAVRERNS
                     LDTALVEDVVLGCVDPVGEQSAVIARSAVIGAGYGKQTPGVQINRFCASGLDAVALAA
                     AQVMAGQKDLAIGGGVESMSRVGMGASGMLPAADPSIAIPSYFSPQGVAADMIATKFG
                     FSRDDLDAFAVESHKRAAGAWADGRFAKSVVPVKDINGLTILDRDETVRPDANMQSLG
                     ALKPAFAMLGEQAGFDAVLIQAHPDVESINHVHHAGNSSGIVDGASAVLLGSKAGGKK
                     AGLKPRARIRGFASVGSDLALILTGPVDVTELLLKKTKMTLKDIDLFEINEAFACVPL
                     HYMLSLDLDPAKVNVNGGAIAMGHPLGATGGMILGTVIDELERQDKAVGLVTLCVAHG
                     MGTAMIVERV"
     gene            25702..27915
                     /locus_tag="PK812_01285"
     CDS             25702..27915
                     /locus_tag="PK812_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HRJ68214.1"
                     /translation="MKLTNFRFETDADGIATLFWDQADRSMNVITIEWMQELNKAIDH
                     IAATPEIKGCVVASGKPDSFSGGADLTMLQATGLQYAKMAKAEGEEKAMAFFFEQTSA
                     LSRLFRKLETCGKPFAAAIHGVCMGGAFELALSCHHRVASDSDRTRLGLPEIKVGLFP
                     GAGGTQRVPRLMQTGDALQMLFKGDQIRPAAARGMGLIHQVVPQDRIIETAKAWIKAG
                     GSAKQPWDTEGYKLPSNKVHSPQGMMIWPPASAIYRKETADNYPAARAILQAVYEGLQ
                     LPMDLALRVESRYFAKILRSPEAAAMIRSLFLSKGELEKGARRPAGIPATKLMTIGVV
                     GAGFMGAGIAYVTALAGMNVVLVDRDQEAADKGKAFSHKILSDQIMKGRAKSADRDAL
                     LGRINATADYAALKACDLIVEAVFEDRAVKAEVIKKIEAVIGKEVIFASNTSTLPITS
                     LAENSARPDQFIGIHFFSPVDKMLLVEIILGKKTGDRALATALDYVRAIKKTPIVVND
                     TRGFFANRCVLNYVREGQLMFMEGVPATMVETVAKMAGMPVGPLSLMDEVALDLGWKI
                     LQATKKDLGAKAIDPVQEALLEEMVVKRGRFGRKNRMGFYDYPEKGPKKLWPGLADVQ
                     KKKLAPATIDIAELKQRMLVVQALEAARTVEEGVITDVREADVGSILGFGFAPYTGGT
                     LSYIDGMGTKAFVALCDKLARKHGARFKPNKLLRDMAEKGETFYGRFAPVRKQAA"
     gene            27938..28480
                     /locus_tag="PK812_01290"
     CDS             27938..28480
                     /locus_tag="PK812_01290"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRJ68215.1"
                     /translation="MNLWLRMLWLALTARFRPRFDTPLGLSRLTLRVWPFDVDVNRHL
                     NNGRYLTIADLGRADLFHRSGVWRQARAMGLFSTMLGVAIRFQKEIKPLRTFDLESRW
                     VYWNERVAVMEHLFLIGAGEGRRVAAREFASCGFYDMKVRRFVDPGDLFRKAGAQGGS
                     PPASEEILAFLKAQETLKQV"
     gene            complement(28498..29094)
                     /locus_tag="PK812_01295"
     CDS             complement(28498..29094)
                     /locus_tag="PK812_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012092648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031966 - mitochondrial membrane
                     [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial cytochrome c
                     oxidase assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HRJ68216.1"
                     /translation="MPDPLNPLRIVRVVAWGLIAALSAVMLYVTLSSEKPAGVAIGGP
                     FTLTAMDGKRLNTAEVMRGRPYGVFFGFTHCPDICPTAMWELSEILRKSGPMAKDFRV
                     FFVTVDPERDTPELLSSYISAFDNRIIGMSGTAEEIAAVARAHRAFYRKVPTPSGYTM
                     DHTATIYLFDGKAQLWGTFDATESEENRLKKLARLLGK"
     gene            complement(29154..30146)
                     /locus_tag="PK812_01300"
     CDS             complement(29154..30146)
                     /locus_tag="PK812_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012329790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1775 domain-containing protein"
                     /protein_id="HRJ68217.1"
                     /translation="MFTTIGRMAAISVAALCLTATAHAHATFDIAEATQDSSFKGVVK
                     IGHGCEGSPTHTVIISVPEGVIGVKPMPKSGWKLKTTTGAYARTYDYFGRPMSEGVKE
                     IVWSDGQLKDSEYDEFVFSSRISGAVPTGPLHFPVTQLCEKGEHRWSEVAAPGVDPHS
                     LKSPAPAVRIKAKQVAQAGGHGSHGTQTQAQHQHHAAPTASGLILHLPWTRATPPGAK
                     VAGGFMTIENKTGTADRLIGGSFEVANVFEVHEMAMEGGVMKMRALPKGLEVPAGGKA
                     ELKPGGYHVMFIDLKRQLKEGEKLKGELVFEKAGKIPVEYIVRPIGSTSGGHAH"
     gene            complement(30190..30552)
                     /locus_tag="PK812_01305"
     CDS             complement(30190..30552)
                     /locus_tag="PK812_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68218.1"
                     /translation="MFRPRRRSLTREIIALALSSVVFIAAVAAARLQGAHPGARAGFS
                     GVLCLSDDTNPEVPGKSPAMHQHCVLCAFAASDCGGERPPETKRFATRAAPDPSSEAA
                     VVVFEVQLFDARGPPALI"
     gene            complement(30594..34166)
                     /locus_tag="PK812_01310"
     CDS             complement(30594..34166)
                     /locus_tag="PK812_01310"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing hybrid sensor histidine
                     kinase/response regulator"
                     /protein_id="HRJ68219.1"
                     /translation="MVDGWLIVLSGLFYLCILFAVAHFGDTAGSRILAGRLRPVIYAL
                     TLAVYCTSWTFLGSVGLAARSGYDFLPIYIGPVLVIAFGWSLLARVVKLGKTQNTTSV
                     ADMIATRYGKDERVAALVALICVVGMVPYIALQLKAVALSLDLISLPGIQFRPPELGA
                     LPFGDGGLWIALILAFFAVAFGTRHIDATEHQSGLMLAVATESVVKLVAFIGVGAFVV
                     WGMFGGAGDLVEKARQNPDIMRALTFAPDAASWLVTTLIAALAFVLLPRQFHVIVVEN
                     RELGDLKKAAWLFPLYLVAINLFVIPIAAAGMLIFPGRVDHDLTVMALPLYANSNLVA
                     AVAVVGALSAATAMVIVETVALGIMVSNDLILPFLLRRSSRHRERGATAVDARMAGEP
                     EPASAADMSGTVLIIRRAAIVVILLLGYLYYRASNEAALASIGLLSFAAASQLAPAFI
                     GGFLWHRGTARGAVAGLIAGILCWAYLLFLPSFAAGNSYVAAFVADGPFGIDWLRPQA
                     FNVFGLTPLVNGVIISLSLNIIAFVGFSLTRHATPIERLQANVFIGVDTGLSGPSFRL
                     WRSAVTVAELRQTVARYLGDERTAREFTEFLTRHGQPENVNGEADAHTIRFAEHLLAS
                     VIGAASSRLVLSLMLKRRNVSTEAALKLLDDASAAIQYSRTLLQHALDHAKQGVTVFD
                     SDLRLVCWNRAFQDLFELPHELVRFGVGLDDIIGYNARRGIYGPSSSPDFIASRLESF
                     VHNTEPVRLRLHPLGKVLEIRSNHLPDGGIVTTYTDITEAVEAEEALSQANESLERRV
                     LERTDELVRVNEELARAKAEADDANLSKTRFLAAASHDILQPLNAARLYATSLVERTD
                     ATPHAQLAQNVGASLDAVEEILGALLEISRLDTGKLRPEWSVFRIDDLFAQLAVEFDP
                     AVRAKGLELTFVPSGASIRSDRRLMRRALQNLISNAIKYTEKGRVLVGCRRRKGMVRI
                     EVRDTGIGIPASKQRIIFKEFQRLDQGARVARGLGLGLSIVERIARVLNHRIAVTSTA
                     GKGSTFSLEAPIASAEPVTFVPPEVTNASQQPLAGLHVLAIDNDPTILDGLRTLLEGW
                     GCVVATAASFDDAKRTLRLAGNPDVILADYHLDNSDGLDLVRRLRKHARIDLPAVLIT
                     ADRSTEVRDRAAEMGMQVLNKPLKPAALRAILVTYRPAQEVAAE"
     gene            34371..34787
                     /gene="mscL"
                     /locus_tag="PK812_01315"
     CDS             34371..34787
                     /gene="mscL"
                     /locus_tag="PK812_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015822930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HRJ68220.1"
                     /translation="MLKEFKEFAMKGNVVDLAIGVIIGAAFGKIVESTVADIIMPIIG
                     MLGNFDFSNLYASLGPKVPAGTGYADAKKLGPVLGYGQFITYVVNFVIIAWVLFMVVK
                     GMNRLKKPEEAAPAAPAEPPADVKLLTEIRDLLAKK"
     gene            34913..36124
                     /locus_tag="PK812_01320"
     CDS             34913..36124
                     /locus_tag="PK812_01320"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRJ68221.1"
                     /translation="MNQHHALSSPASRWRIGEAAQAAPTSGIVEVFNYGRNRPGLLPL
                     WVGEGDRKTPDFIVEAAQKSLRDGETFYTYQRGIPALREAIAAYMSDLYGQSLGADRF
                     FVTIGGMHALQIAMRIVAGAGDEVIVPTPAWPNFIGAIVANGSISRPVEMPRTATGFV
                     LDFEALEQAIGPRTRAICLNSPANPTGWTATRADQERLLDIARRHDLWIIADEIYARF
                     FYQGFRAPSFHDIMAEDDRILFVQTLSKNWAMTGWRVGWIEAPPALGQVIENLIQYSS
                     SGVAVPLQRGAAAAIAQGGSLIAEQVELARANRTLLLDTLRRNNALVIPEPTGAFYLF
                     FGIEGLLNSTQAAFRLIDDANLGLAPGASFGPGGEGFMRLCYLRREDDIAEAARRLVN
                     NLERIRSGVSA"
     gene            36121..37194
                     /locus_tag="PK812_01325"
     CDS             36121..37194
                     /locus_tag="PK812_01325"
                     /inference="COORDINATES: protein motif:HMM:NF016999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HRJ68222.1"
                     /translation="MIDRRGPILATVQTLAAGIAGGWVFSILGLPVPWLAGPMAAVAA
                     LAMSGQSVRLPRLLCDTGLFVAGISLGSTVSPEALAAVSRYPASIVALVLSVAAIMVV
                     SMVLLELAFGWDRQTAYLASVPGALTMTMALVAETRADAGRVAVVQAIRLFALVALLP
                     ILITGSAASAPTSGQTVMTPAGMACLCLVALALDALLTRIGAITPLFLGGMISATVLH
                     VSDLVPGALPEPIVIVGFLLIGVYAGLRFDGMTVAVMVRVLVPGLVSLAATLAVTVAV
                     AWLVVGLAGVAPAEALVALAPGGVEAMILIGASLGLDTLYIGTHHVVRLLVLNALTPV
                     FVPRHPRSETEHRPHSANPPSAR"
     gene            complement(37164..37910)
                     /locus_tag="PK812_01330"
     CDS             complement(37164..37910)
                     /locus_tag="PK812_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRJ68223.1"
                     /translation="MAGRLKGKVAVVTAAGQGIGRAIAEAFAAEGAKVYATDVEVSKL
                     EGLARVKKRKLDVRSSRAVRMLAEKTGPVDILVNAAGFVHHGTVLDCSEDDWDFSFDL
                     NVTSMHRTITAWLPGMLAKGSGSIVNIASGASSVRGIPNRYVYGTTKAAVIGLTKAVA
                     ADFIKKGIRANAICPGTIQSPSLDDRIAALAKSTGQTAKAVRQAFIDRQPMGRLGTAE
                     EIAMLAVYLASDEASYTTGQIHLADGGFAL"
     gene            38126..39313
                     /gene="metC"
                     /locus_tag="PK812_01335"
     CDS             38126..39313
                     /gene="metC"
                     /locus_tag="PK812_01335"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009492603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HRJ68224.1"
                     /translation="MTKMTPEKKAKLRSSSKLVHLGRDAAEQFGYVNTPIYRGSTVLH
                     PTVEALKGRTQRFSYGTRGTPTTEALESAWADISGAAGTVLVPSGLAAVVLALMAVVK
                     AGDHILVVDSVYRPTREFCGSMLARYGVETEYYDPAIGARIADLIRPNTSLVFTEAPG
                     SQSFEMQDIPAIAAAARARGCCVIMDNTWATPLFFPPHERGVDIAIEAGTKYLGGHSD
                     ILLGLVTANAQWLPKIKDVFIATAMCPGPEDVFLALRGLRSMELRLKHAEQQALGLAR
                     WLEQRSEVARVLHPALESHPGHAIWKRDFLGSSGLFSIILKPCSEKAVAAMLDGLELF
                     GMGYSWGGFESLVIPFDCASYRTATQWTPGGPALRIQVGFEHLDDLKADLEAGFERLR
                     KTG"
     gene            39353..39934
                     /locus_tag="PK812_01340"
     CDS             39353..39934
                     /locus_tag="PK812_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRJ68225.1"
                     /translation="MHWYLTHPQVRIDPAIPVPDWGLSDVGRARIMTLAGAPWIRRFG
                     RIVSSGERKAMESAQAVGLAIGVPVELAEASHENDRSSTGFLPPQEFERMADAFFASP
                     QDSVRGWERAIDAQARIVAVVRQVLQARPRTPTLFVGHGGVGTLLKCWLRGRDITRAE
                     DQGPGGGGNHFGFTLSPKGVLHDWLPLETPPPG"
     gene            40012..40215
                     /locus_tag="PK812_01345"
                     /pseudo
     CDS             40012..40215
                     /locus_tag="PK812_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014298101.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2093 domain-containing protein"
     gene            complement(40179..41216)
                     /gene="lpxK"
                     /locus_tag="PK812_01350"
     CDS             complement(40179..41216)
                     /gene="lpxK"
                     /locus_tag="PK812_01350"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HRJ68226.1"
                     /translation="MKAPRFWWTRPPAVIARLLAGIGWIYGWLTARRMEQSGARAVVP
                     VICIGNFVVGGAGKTPTALAVARALQASGQTPAFLTRGYRGAHRGGPLAVDPAVHKAS
                     EVGDEALLLARAASTITAVDRVAGAQAATALGASVLIMDDGLQNPTLEKDFTIAVVDG
                     SRGSGNGLCIPAGPLRAPLAAQVGKVDAILVIGLGHGMAEAAELGKAAGKPVYRTEFL
                     VDDAIAQQFGGQKVLAYAGIGNPEKFHQTLRGLYAKVVAARSFPDHHAFTDHDAQSLL
                     DEARAQGLMLVTTEKDAARLSGSPALEALRAETTVLPVSLRLPLALIDALVSVAGPPA
                     RTRLSEISAEA"
     gene            complement(41213..42505)
                     /locus_tag="PK812_01355"
     CDS             complement(41213..42505)
                     /locus_tag="PK812_01355"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HRJ68227.1"
                     /translation="MSGRDPLSLHLHRLATTVIEPAMPAFLRSRQKRGKEHAERMPER
                     LGLTTLKRPDGPLVWMHGASVGETVSLLPLIDALVAKRFNVLLTSGTVTSAEVAARRL
                     PAGALHQFAPVDTPTAAERFITHWQPDLALFAESELWPNLIRIASASGTVLGIVNGRM
                     SPRSFRTWRKAPRFIGALLHRFALVLAQSEADAERYRLLGATRACSVGNIKFDVAAPP
                     ADPATLRSLRGQIGERPVFIAASTHPGEEEAVIAAHISASDHLPALLTIVAPRHPERG
                     PHLETSASRAGLSTARRSRGEAITPKTDLYVADTIGELGLIYRLGRVAYLGGSLIEHG
                     GQNPIEPAKLGLAILHGPHVFNFTDVYAAFGESGATDLVTEPEQLGPAVARRLDSMGT
                     LDRMGAQAMLTATALGGAVGRTLNALEPHLTHLRSRTT"
     gene            complement(42507..43262)
                     /locus_tag="PK812_01360"
     CDS             complement(42507..43262)
                     /locus_tag="PK812_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRJ68228.1"
                     /translation="MLKEIWRSRPVQEAMGSLLAGYLTLVRRTNSMHFDPPDAYAGIE
                     ADQPVIVAMWHGQHFMIPFFRKPHHRISVLISRHGDGEMNAIAAHKLGIGLVRGSGAQ
                     RGDQVRKRGGIKAMFDMISVLERGENMALTADVPKVSRVCGKGIVTLAQKSGRPIYPL
                     AVVCSRRIDLKSWDRSSIGLPFGKCGIAVGQPVHVAEEASEAELETARLAVEAELDRV
                     HAVAYALVGSKDPGAGRASVIEARAVAAAEAVR"
     gene            complement(43273..43503)
                     /locus_tag="PK812_01365"
     CDS             complement(43273..43503)
                     /locus_tag="PK812_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4170 domain-containing protein"
                     /protein_id="HRJ68229.1"
                     /translation="MPDKQLLHLVLGGELTSLDSSEFKDLSKVDVVGLFPDYKSAHAA
                     WKAKAQQTVDNAQMRYFVVHLHRLLDPSTDAH"
     gene            complement(43540..44346)
                     /locus_tag="PK812_01370"
     CDS             complement(43540..44346)
                     /locus_tag="PK812_01370"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HRJ68230.1"
                     /translation="MMATASPTPYLGLMRRAAQAASEAALRHFRAGSNTTARVSWKEG
                     GSPVTEADHDADAAIRAVLSASAPDVPVFSEEAAHELQRLHSRRVFVVDPIDGTRAFM
                     QGRDEWCVSIAMMEDGRPVAGVVHAPARNETFEASSGSGAFLNGARLPIIAGQQDRPI
                     RTAGPRPLIERIVAADPELSASEPLRALAYRLVAVASGGITAALASKGAHDWDIAAAD
                     VILAETGCVLLSASGERPVYNAPDPVHPALVAAPPALVERLLALLERMPA"
     gene            44597..45046
                     /locus_tag="PK812_01375"
     CDS             44597..45046
                     /locus_tag="PK812_01375"
                     /inference="COORDINATES: protein motif:HMM:NF039656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6101 family protein"
                     /protein_id="HRJ68231.1"
                     /translation="MTGPFEGLSPARFALAGEGARPPRPANDNQPGTKGAQGFHGLLL
                     RRDGEKYEILLCHREPGLSQRVETVDDDVDVIALWRGIGRKLNLPLFAETADGEIVQM
                     EPMPCLVAAPRRYGSPLSGRRPRFLACRKMGAPRPDAKTRAGAQRAL"
     gene            complement(45031..>45814)
                     /gene="ubiA"
                     /locus_tag="PK812_01380"
     CDS             complement(45031..>45814)
                     /gene="ubiA"
                     /locus_tag="PK812_01380"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HRJ68232.1"
                     /translation="LASAAAGQWPHVGHFLLFLAGAIVMRGAGSTWNDIVDRDLDGRV
                     ARTRGRPIPSGAVSARQAAAFMLALCLAGLAVLLTFNNFTVMLGFAALLPVVVYPFMK
                     RVTNHPQIVLGLAFAWGGLMGWAAIFGKLEAPALLLYAAAITWTVGYDTIYALQDVED
                     DSLVGIGSTAIAWGPRAPALVAGMYALTVGLVGAALWYALPDGAPIAWFFLGLFAAHL
                     LWQVRALRLGDWLLALRLFKSNRDSGLILTAGLVLESAYRAL"
BASE COUNT         8443 a        14250 c        14698 g         8223 t
ORIGIN      
        1 tcgcgcatct gtgctgcttt gccgaccttg ccgaactggt cagacagggc gaacacgacc
       61 gccatggcat ctacatgatg gttcccggac tgctgcggca gatgcgcacc ggagcaatcg
      121 ccggactggc cgcgccacgc ccgcagcttg cctgcatggg ggccaaggat ccgttgaccc
      181 caccttccgc catagcggtg gcagtcgccg atgtccgggc atcgtatgct gcccgcaatg
      241 cactggatcg gctgactatc acgattgacg cggaagctgg ccactgtgag acccctgaaa
      301 tgcggacggc ggtgctggat ttcctgacac ggcagctttg aacaggcggg caggttgacg
      361 ctcttgacgc gcgcacctat tagccagagt gcaatgaacg acgtctcgat ccgtcgcagg
      421 aattcctctt tgcccccttg gcgggagtga ttccttggaa ggcaccagtc tgccgggaat
      481 tcgtctcctc aggtttggat gctcgggatc agacgcggcc atcgcccttg tgctgcgggt
      541 gcagcgatgt cccagcgacc catccgattg cgagccatga ctttcgatcc cgcccttcgc
      601 gacgcagctt tcagcgccaa cgcctggccg tttgaggagg cccgcaaact ggtcgctcgc
      661 gtcgagaaaa cgggacaaaa gcaggttctg ttcgaaaccg gctatgggcc ttcgggcctg
      721 cctcacatcg gcaccttcgg ggaggtggcg cgcacgacca tggtgcgccg cgcattcgag
      781 ttgctcacgg gcgggacgat cagcacgcgg ctgatcgcat tttccgacga catggatggg
      841 ctgcgcaagg ttcccgacaa catcccgaac aaggacctga tcgccgctca tctcggcaag
      901 ccgctcacgg aggtgccgga cccattcggc acgcatgaga gctttggcgc gcacaacaat
      961 gcccgcttgc gcgcctttct cgacgccttc ggctttgaat acgagtttct gtctgccacg
     1021 caatgctatg cctcgggccg tttcgacagg acgctgctgt tcatgcttca gcgtgtcgag
     1081 gcgatcatgg agatcatgct gccgagcctg cgcgaggagc gggctgcatc ctattcgccg
     1141 ttcctgccga tccacccgga gactcgcgtg gtgatgcagg tggcgattga aaaagttgat
     1201 gcacgggcgg gcaccatcgc ctggcgcgat ccggacaccg gaaaggcctt cgagacaccg
     1261 gtcaccggcg gtcactgcaa actgcagtgg aagcccgact gggccatgcg ctgggtggcg
     1321 cttggcgtcg actacgagat ggcaggcaag gatctcatcg actcggtgaa actgtcgagt
     1381 gcgatcgccc gcgcgctgga ttcaacgccg ccggacggct tcaactacga gcttttcctg
     1441 gacgagaagg gccagaagat ctcgaaatcg aagggcaatg gcctcaccat cgacgagtgg
     1501 ctgacctatg ccagcccgga gagtctgtcg ctgttcatgt atcagaagcc gcgcgaggcg
     1561 aagaagctct acttcgatgt cattccgcgc tgcgtcgacg agtatctctc tttccttgac
     1621 gcgtatccgc gacaggacga aaagaaccgt ctcggcaatc cggtctggca cattcacagc
     1681 ggcgctccgc ctgcgccgga acggatctca aacgaagcga acggacgtgg cagcggcatt
     1741 tccttcgcgc ttctgctcaa tctcgtcgcc gtggcgaacg ctgaaacgaa ggatgtgctc
     1801 tgggggttcc tgaagcgcta tgcgccgtcg gcgtcccccg ccacccatcc acaattggat
     1861 gcgctggttg gctacgccat tcgctactac acggatttcg tgaagccgaa gaaagtctat
     1921 cgcgcagccg acgcagacga gatcgccgcg cttgccaggc ttgccggtcg tctgcgggcg
     1981 ttgccgccgg aggccacatc cgaagcgatc caggaagccg tctacgacgt tgccaggcca
     2041 attccgcgct tccaggacat gaaggcgaag ggggccacgc ccgagcgccc gggcgtatcg
     2101 aacctgtggt tcaatacatt gtaccaaatc ctgctcggcg aggagcgcgg cccgcgcttc
     2161 ggttcgtttg ccgccatcta tgggctgagc gagaccgcga ccctcatcga tcgcgccctt
     2221 gccggcgagt tgatccgcga gcatgaggcc tttgtggcga aacgtgccga ggggtgaggc
     2281 acgtcgtccc tcccggcttt gcccgctact ggctcgccca gaatatccgt gccacgaaga
     2341 cgatgtcgtc gcgggggaag aactgtgacg ggccactggc ttccgttcgg gcaagatcga
     2401 tcccattggt ggtttcgcgc ctgacctcgc cgagcaccat gcccccgtcc ttcaaccgaa
     2461 gcagcacccg gtcgccgcgt ctgacctgcg catcgcagga cgccaccaac acggttccct
     2521 cgccatagag cgtcgaagcg ctgggggaat caatctcgac cgcaaaggac ttccttccct
     2581 gaaacccggg cagggcgatc tcatcccagg ttccgcccgc tggctcgcca agttcggaga
     2641 aggcggcagg gctggcggcc tccgacaggc tgatgaccgg cattcgcaac ccggccgccg
     2701 gatcctgtac ggaaattagg gacacgaagg tgtcgaggct ctcctgcgtc gccatcagga
     2761 tcttggcaac ggattccgtc gatggccagc gctgacgacc gtcgggcgtc agtcgcttgg
     2821 aacggttgaa tgtcgtcgga tcgagacctg cccggcgcgc aagaccggac gccgaaagtc
     2881 catggcgtgc ggcgagcgca tcaatggcgt gccagatctg ctcatgcttc aacatggaat
     2941 gccttatatc tccaatcgcc tcgatcaact gaggtaagta tgtctgaaca ctgcatgaaa
     3001 gcagccctgc atggtgcgtt caaaaaagtt gtttgctcca aattcgaaca cttggcaatg
     3061 agggtgccga agcggggaaa atcctgatgc cactcgtttt caagatcgtt gaccgccatg
     3121 cctggcagcg tgcggaggcc cttgggtctt ttgatggcgc gcccgttgat ctgcgggatg
     3181 ggtacatcca cctgtcggcg gctcaccagg tgcgcgagac cgcagcgaaa cactttcgcg
     3241 gacaggacga tcttctgctc gtcgcataca caagcgaggt tctcggaaag gatttgagat
     3301 gggaggcctc gcgcggtggc gacctgttcc cccatctcta tggnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnaggattt gagatgggag gcctcgcgcg gtggcgacct
     3481 gttcccccat ctctatggat cactgccgac cgcgaaagcg ctttgggcca agccattgcg
     3541 gcttgacgcg gaccgcgagc atctgtttcc agacctgtca tgatcacttc gctgctgttt
     3601 cgcgctgctc gtccggccct cttcgtcctc gacgccgaga cggcgcacca ggcgtcgctc
     3661 accgcattga agttcgcgcc ggtgctgcct tgcgcgccgg acgatcccct ccttgcaagc
     3721 gaggcccttg gcctgagctt tccaagcccg gttggcctgg ccgcagggta cgacaagcac
     3781 gcggagatac cagaccgatt gctcgcactg ggcttcggtt ttgttgaggt aggggcggtg
     3841 acgccgcgtc cccagccagg aaatccgaaa ccccgcgcgt ttcggctggc aggcgatgag
     3901 gcggtgatca atcgctatgg tttcaacagc gagggtcttg aagtcgtggc agcacggctc
     3961 gaacaccgga agcgccgcgg cattgtcggc gtcaatctcg gctcgaacaa ggatacgagc
     4021 gaccgggcgg cggactatga aattctcgtc gcgcgcctgg cgcactgcgc cgacttcctc
     4081 acggcgaatg tgtcttcacc caacaccccg ggcctgcggt ccatgcagaa ccgggcgcag
     4141 ttcgatgatc tgctggctcg cgtcgtcgcg ggtcgtgatg ccgtttgcgc cggaggcagg
     4201 cgcacgcccg tgctggtcaa gatcgccccc gacctgacgg atgcggatct cgacgatgtg
     4261 acggatgtag cgctcaagcg gggcatagac ggcatgatcg tttccaacac gacgatctcg
     4321 cgtcctgaaa gcttgcggga gacggcgctt gcgaaggaga cgggtggact ttcgggacgc
     4381 ccgttgtttc gcctgtcaac ccaggtgctg gccaaggtgg cgctgcgcac cgagggacgt
     4441 ctgccgctga tcggcgtcgg cggggtcgat tccggcgaag cagcgctgat gaaaattcga
     4501 gccggcgcca cgttggtgca gctctacacg gcgctcgtct accatggccc gggcctgatc
     4561 gacgacatca agcgccacct ggcgacaacg gtgcgcaagg gtggccatac ctccctcgcg
     4621 ccactgattg gcagcgcggc aaaggagatc gcggcaggag cgttctgagc ctcgtctact
     4681 tgcgcgccag cagccggatc agcagccaca gcggcaccac aagcacggcg ccggccgcaa
     4741 tgtagccacc catggtgtac aacgcctcga tgccgcttcc gaaaatctca tgcaccagat
     4801 agcgcaggta gtgcaggaag ctccacggcg tgaagccgaa ggcggccagg atcaccccga
     4861 ccagcagcga cagcagtaca agcttgatca ggaccgagac gggcgatcca cccaggaact
     4921 gctgcacgcg tgaatccgac attcgactgc ctccacccat agggcattcg acatctgctc
     4981 tatcgcatgc ccgctgagtg gcaagtgaac gccgacgcgc gccgcgctca ccaccactct
     5041 gatcattctt gcaggatttt taccttgagc gggttgaagg cgacgtagac atcctcgttc
     5101 tttcgccagg gatcggggcg cacgtcctcg acgaggaaga cctcgcccac atcggtctcg
     5161 acggtatatt ccatgtgctt gcccagatag gcaaccttgg caaccttgcc gcgcacatgc
     5221 ggttcggccg gtttcttgca gtagatgcgg gcggctgtgg ggcgcacgac cagtgtcgcc
     5281 ttccccgttc gtccattcga agagggcagg tcgagcatcg cctcgcccac gcggaccttg
     5341 gcaaaaccag tcgtgacgga cacgacttcc gcctcgatca ggttcgaatc gccgatgaag
     5401 tcggcaacga acaggctcgc cggctcctcg taaagctcat gcggcgcgcc aacctgcgcg
     5461 atgcgcgcat tcgacatcac gatgaccttg tcggaaaccg cgagcgcttc ctcctgatca
     5521 tgcgtgacat aggcgacggt aaggttgagc ctgagctgga gatccctgat ctcctcgcgc
     5581 acccggcggc gcagcttggc gtcgagattg gagagcggct cgtcgaacag cagaacctgc
     5641 ggttccagta ccagcgctcg ggcaagggcc acacgttgct gctgaccgcc ggagagctcg
     5701 gacggaaagc ggtgttccat gccggccaga ccgagcatgg tgagcttgtc catggccatc
     5761 tcgcgcgctc ttgctttcgt cgcgccctgc acggaaggtc cataggccgc attgtccagc
     5821 acgctcatat gcgggaagag cgcataggac tgaaacacca tcgagacgtc gcgatcggcc
     5881 gcggacaggt tggtgacgtc cttgtctccg atgatgatgc gaccgtcgct cgccgtctcc
     5941 aatccggcaa tcatgcgcag cgacgtggtc ttgccgcagc ctgaggggcc aagcagggtt
     6001 accagcgtcg ctggcgctat ggtgaaggag acgtcatcca cagccaccac gccgccgtag
     6061 cgcttggtga cgttgcggaa ctcgaccgat gccggctgct tggccatggt tcttgctttc
     6121 taaaaaatca acctgccggg atcggcatgg tcgcaggccc gccccccgtg ccgcgccgtc
     6181 ccagcttgcg ctcgcccacg gcgaggccga tcagcgtgat gacgatgccc atgatcacaa
     6241 tcagcgccga ggaataggcg attgcgagcc cgtgctcgcc tgcgtccacc cggccgacga
     6301 tgtagatcgt cgccatgttg tactcggcgg aaaccaggaa aatcaccgca ctcaccgcgg
     6361 tcatggcgcg cacgaatgca tagaccagcg agacgaggat ggcaggtcgg agcaacggca
     6421 gcatcacacg gcgcacagtg gtcagcgacc ccgcgcccag cgtcagcgag gcctcgtcga
     6481 ggctcttgtc gatctggctc atcgccgcga cgccggcccg cacgcccacc ggcatattgc
     6541 ggaagatgaa gcagatgacg aggatcatcg ccgtgcccgt gatctcgatc ggcggcacat
     6601 tgaaggcgag gatgtaggag acgccaacca cggtaccggg aatggcgaag gacaggagcg
     6661 tgccgaactc gaaggcccgc tgcccggcga agcgctggcg cgtcagcagg taggccgtga
     6721 gcaatccgat cgcggcggta aagggcgcgg cgatggcggc gatcttcatg gtcgcgaaca
     6781 gcgagttcca ggcgctgccg gagaaaacga gcccgaactg gccctgttcg atcttgaatg
     6841 cggtgatgta gtgttgcagc gtcggcgtgt agtcgcgtcc gacggtcttc acgaagccgg
     6901 cgacaaggat aagcgcatag acggccaccg tgaacaggcc ccacggtatc gccacgaggt
     6961 aagcgcccca ttctacgcgc ttgggcagag gcagttgcag gccggaatcg cccttgcccg
     7021 tgatggttgt atagaccttc tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7141 natggttgta tagaccttct tgcccaccca gagctgttgg gccgcaaagc cggtcagcgt
     7201 gaacacgagg agcaccagcg acagcaccgc ggcgcggccc tgatccgtgg aggcccccac
     7261 cacggcaaag aaaatcttcg tcgacagcac ctcgaaattg ccgcccagca ccagaggatt
     7321 gccgaagtcg gcgaggcttt cgacgaaacc gagcaggaag gaattggcga gacccggccg
     7381 catcagtggc caggtcagcg tcaggaagat cgtccaggac ttggcgcgaa gcgtctgcgc
     7441 cgcctcctca agcgttggcg agatgccctg gatgacgccg agcatcacga gatacgccgg
     7501 cggcgtgaag gccagtgtct gggcgataaa tacgcccggc aacccgtaga tccagcgcga
     7561 gggcggaatg ccgaaccact gaaaaagcac gctggaaact gcgccggagc gcccgaagat
     7621 cagaatgagc gccaggccga tcacgaacgg cggcgtgatg atcggcagga tcgcaagcag
     7681 cttgaggctg cccttgagcg cgaagccggt gcgcgccgaa atcagcgcga aggcaagccc
     7741 gagcagggtc gatgagatgc cgacgagcac ggccagaaac agtgtgttcc aggcaacgcc
     7801 gcaagcagtg ccgccgccca gacacccaag cccccagatg gacgaatccg tcaccttctg
     7861 cgcgaacagg tgcggcgcga aatgcccggc gttgtcttcc accgccgagg acaggattgt
     7921 cagcacagga aagaacacga acagagcgat cagggccacg atccagccga tggacgcgac
     7981 cacgaaaagg tcgccccggc accagccgcg cccggcgagc ccaacgcacg cgagcatcag
     8041 gaaagcgctc aggaccacaa gcgcgccggt gcccatgccg ctttgcgacg gccccgcccc
     8101 gaaaagggtg gtgaccacat caaggttcca gccttttaac ccgatcagaa atccctggcc
     8161 cagaacgact acgagtccga agatgccgcc gacggtaaga ctggtcgcaa gcgccgcctc
     8221 gcgcctggag cgcagcagaa ggctggcgag caggccagcc agcaccagca ctggcaggat
     8281 cagccagaac cgcgaggaaa gcgccagggc gagcgctgaa tgagcgggat tgacgatctt
     8341 gcccaaggtc cccgcccccg gcggctccag gtaccacggg cagatcgcaa cgccgactag
     8401 cgccacgcag caccagaacc acccccgtcg catcatgatc gccgccccac ccatctcaga
     8461 caccgctcag cgcggcaggc tcttaacctc ggcatcccac ttgcgcagga gccgcgtgcg
     8521 ttcggccgaa gagccatact tcgccgtatc gtaggtgatg agcttcatgg tcgagaaatc
     8581 cggagcgtcg gccgggacct tcacgttcct gttggatggc atggaataga tcttcaggcg
     8641 attgttgatc tcctgcgcct cggttcccag cgcgaaatca tagaacctgc gcgcggcctc
     8701 cgggtttttg ccacccttga tcagggagac cgatccggtt tcgtatcctg tgccttcgca
     8761 gggcgcgacc gtcttcagtg gtgcacccgc caacttctgt gtgatcacgt cgtgcaggaa
     8821 ggaaatgccg acaccggttt cgccgagctt cagtgcgttc accggcgcga tgcctgactt
     8881 ggtgtattgg ctgacattcc tgtgcagggc cttcatgtac tcgaagccct tgtcttcgcc
     8941 catcacctgc acgatcgtgg caacaaaggt gtaggccgta ccggacgaat tcgggtcggc
     9001 catttgaatc tcgtccttga acttcggatc gagcaggtct gcccagcact tcggttccgg
     9061 gatgccgcgc ctggccagca cttccgtatt gatcccgagc cccaaggcgc cgagataggt
     9121 gccggcggtg cggtatttcg actgctccgc atgccgcacg gcccaaggct gcaattcacc
     9181 aagtttttgc gacttgtact cttccgtcag gccttcttct gcggcctgga gatgcgcgtc
     9241 gcctggtccg ccccaccaga tgtcgcccct ggggttggag gcttccgctt tgattgtcgc
     9301 gtaggtctcg ccggtgctct tgcggatcat cagcactttc gtgccggtcg ctttctcgaa
     9361 cattgccgcg ccttcgcggc actgctcctc gagaatggag cagtagagag tgacttgggc
     9421 ctgggccatg gctgtcgtcg ccaatgccgc cagaacaata gccgccatgc cacccgcgat
     9481 aacgggctgt ctcatgatgt cctcccggtc tttgcaaaaa ccggcgacag ccttctgccg
     9541 ccggctgtga aacgtcgcgt cacttcggcg cgttcttcac ttccttggcc cagcggtcga
     9601 gcagtgcatt gcgggtggcg gaagagccat acttgttgaa atcgtagttg atcagcttgg
     9661 tctggctaag atcgacgccg aatttcggca ggggcgtcgc cttgttggat ggcgtctgga
     9721 actgcttgtt ctccgcgccc agcgcctgcg gcgcggcggt cagcgcccag tcatagaact
     9781 tcttggccgc atccgggttg cgcgcgccct tgaccatgga catcgagccg atctcgtagc
     9841 cagtaccttc gcagggggcg acaaccttca cgtccgggtt ttcgcgcgcc atcgcgatca
     9901 tgtcgtgcat gaaggtgatg ccgattgtcg tttcgccaag gctggtcgcc ttggcaggcg
     9961 cggcaccgga cttggtatac tgattgatgt tcttgtgcag agccttgagc cagtcgaagg
    10021 ccttgtcgtc gccctggatc tgcacgatcg tcgcgagcag ggtgtaggcc gtcccggatg
    10081 agaacgggtc ggcgacctgg acctcgtcct tgtattccgg cttcaccaga tcggcccagc
    10141 acttgggttc cggcagcttc ttcgccttga gcactttctc attgtagccg aagccgagtg
    10201 cgccagtata gatgccgatc gaacgacctt ttgtcgcctg gtgctggcgg atcgcccaat
    10261 cgtgcagctg ggcgttcatc ggtgatgtgt actcctgaga cagcccttcc tcagccgctt
    10321 gcatgtgcgg atcgccggtg ccgccccacc agatgtcgcc gcgcgggttg gcggattcag
    10381 ccttcagctg ggcatagaac tcgccagagc tcttgcgtgt catcgccacc ttgatgccgg
    10441 tatccttctc gaacatcgtc gaaacggctc ggcaccattc ctcctgaacg gcgcaataca
    10501 gcaccagatt cccctgcgcg tgcgctgggg cgatggcaag cgaaccaagg gcaagtccgg
    10561 cgagagcgcc ggcgcggacg tgtgtcttga acattatcaa cctcccggga caggcacatc
    10621 gttgcagcgt gccgtttgaa gcgatttaga ccgcacgcca accccagcgt caatgactgt
    10681 tgtgacagtt tgatgatccc gttccccttg ccaattgtgc tccggcgtgg tgcctgagcg
    10741 gatcgccgtc ggacaaggga agagctgcga tgaaggtgcg tgttacgtgg gctgacaaca
    10801 tgatgttcgt cggcgaaact ggttcaggcc atgccctggt gatggatggc gcgcccgaag
    10861 ccggcggcaa gaacctcggt gtgcggccca tggaaatgct cctgttgggg cttgggggct
    10921 gtaccgcatt cgacgtggtg ctgatcctca agcgtggccg agagccggtg aacggctgtc
    10981 gggttgacgt cgaggcggag cgtgccgaga gcgaaccgaa gatcttcacg aagatccata
    11041 tgatctatcg gctgagcggc aagggcctgt cgccggtcaa ggtcgagcgc gctatccagt
    11101 tgtcgaagga gaagtattgc tcggcttctg ccattctcgg agcgaccgcc gagatcacgc
    11161 atgagttcac ggtctcggac gagcgggagc tgaacggatg agcgttcctt cccaggggct
    11221 ggttgcgccc catgtgctcg ccgcggtcct ggccgatccc ggcgtatttg tcctcgattg
    11281 ccggagcggg ccggacgggg cgggacgtgc ggcgttcgaa cagggacaca ttccgggtgc
    11341 agcacacacc gactacggcg cggacgcttg gcgggtgaaa atcggcggcg cgcccggcat
    11401 gatgccggag cgagccgaag tcgccgggaa gctcgggcgc ctgggccttg aaccctacca
    11461 caggctggtg atctgcccgg ccgggctttc cgccaacgac cttgcggctg cggcccgcat
    11521 cgcctggagc gcgaggatgg cgggccatcg cgccgtcgcc cttctggatg gcggctttgc
    11581 cggctgggtc gcagcggggc tgccagtggc caccggcgct tccttgccac gaccagcgac
    11641 agactaccct ctcgcgcccc cgaccgccca gcgccgcagc gcagccgagg ttctcgccgc
    11701 cgtgaccaag ggcaccgcca cgctggtcga cgggcgcgcc gagagctatt atcgcggccg
    11761 cgagaaggct agcgaggcgc ttgcagccgg gcatatcccg ggtgcgatca acatcgacta
    11821 tgtgatggcc tacgacgcct tgaccaaccg gttgaagtcg cctgcgcaac tggcgaccgt
    11881 ctatgccacc ctcccgccgg gcgctgtgat cagctactgc aatacggggc atacggcagc
    11941 gcttaactgg ttcgtccttg ccgaggtgct cggacgcgac gatgtcgcgc tctatgatgg
    12001 ctccatgacc gattggaccc aggacatgag ccggccggtt tctatctgac aggcgtaagg
    12061 acagcgacga ggccgggaac atctacgcca gcgtcgcgcc gcgtcgacgt gatgtcgaga
    12121 acggcaagat ggtagccggc tgcctgtgtg gcctgcctca gataggcttc ggagtgggaa
    12181 aagcgcaggt cggcgccgag gctgtaaccc ttgccgtctt gccgctgggc cgtgagggca
    12241 agcaggccat gatgtcccag tcgtggccgc gcggtctcca gaagcggcgc aagatcgccg
    12301 atatagacga caacatccgc cgctatgatc aaatcgtagg tctcgtgcga tgagccgagc
    12361 gcggcgaccg cgtcgctgtt gcgcaaggat cggtagagcc cggtgtcccc tgctttggcg
    12421 agcatccgtg acgaaagatc gacaccgtcg atcgggcctg cgcgatcctg cagcgccgcc
    12481 gccatcagcc ccgtgccgca gcccagatcg agtgtcatgc cgaatcggtc ctgccgacca
    12541 agccggaaac aggctttgtc cagcgcggtg cacagaaccg aaggtccccg ataggcgagc
    12601 gttcccgtca ggtgtttgtc gaagcggtgg gcataggcgt cgaacaagcc ggcgacatag
    12661 gcttcaggca gggcaaagcc gtcctgcatg ccatcgaggc gggcaaggag aagggacgca
    12721 ccaagcacat cggccggctc cagttcgagg cagcgccgca acgcctcgcg tgccacatcc
    12781 gtctgtccgg cttccatcgc ggcgcggcca taggcgaacc atcccgttgc ccagcccggt
    12841 gcgatgtcga cggcctgtcg aaacaggtcc gccgctgcgg catgctcgcc ttcctcccgc
    12901 aagccttcac cccacagata ccgccggtcg gcggccaggt ggccggaggt gcgcaagtcg
    12961 tcggtcatgc gctgcggtcc aggatcagcg ggccgggcgc tgctccttcg atcacggcga
    13021 gaacatgctc ctgagccttg gaagcaggga acacagcggc cgcgaacgcg ccggcgcaga
    13081 gggagcaggc gatgcccgcc agcacgcccg atggcccctc gagaacgccg gtccacaccg
    13141 ccgcgccagc cagaagtgtg ctggcaagac cgcagagcgc ggcgcgccag tccgcccggc
    13201 gccagcgtgc cagcaccacc aaaggcagtg ggcagcatgc ggcagcaaac agtaggagcg
    13261 aaacgaggcg cggatcaacc gtcgggacat agcctggacc atagagtgct gcagcaagaa
    13321 caagaagcgc cgccatgcgc tggaccgcga gatctccccc cgccgttgcc ggaggaagca
    13381 cgaaccgcct caggagatcg tgggtcagcg acgccgcagc cgcatggcag gcgagggcaa
    13441 gcccgacgag aagcgcgagg ggagcagcga gcgcaaatgc ttcgccggcc gccgagggaa
    13501 tcgcgagaat cgtggcgtac caatgtccac cccgcttatc gatctgcaca gctgacctcg
    13561 gcagcccttc gctgtgtccg gccctggcgc agatgcgttt caggtcgtcg ggtgtggtcg
    13621 gttcgcttcc gcagatccgc acgagcccac ggaaccggtc agtaaacacg atcgggggca
    13681 tggtctgaac cgaagcgctt tcgatcatgg cgacccgctg ccgttcgacc tcatgggcga
    13741 agacgcccgc gaacgaaatg gctatcaccg ccattaccgc gaaacagccg acttgcgttg
    13801 atgctttctg gctcctgact gttccggaca ggttcaggac cgtaaacagg ccagctgccg
    13861 caagggcaag cagcggccag tcggctccag ctgcgctccc cgatgtgaag gccgttccgg
    13921 cgtgttcgag ccagtgggtc cttgtcgatg aatccgcgaa catcgccggt aggatcggcg
    13981 atgcccaggc gccaacgatc agcgcagatg aaagcaatgc cgccgccgcg acgccggcca
    14041 gtccgcccgg aacgatcacg ctcgcgcaca gggtcagtac cgtcaggctt gcccattccg
    14101 gcttcaggtt cccggccatc tgaacgcccc ggagcgtcgc gggcgccagc cagaccgcca
    14161 atccgccaag catgcagaat gacaggatgc cggcgatcgc cgtgagaagg cgtgacccaa
    14221 atcgcgccgc gatcgcatcg gcgaacgtat agccccccag gcgacgtagc ggaccggctg
    14281 cggtggccga gaacaggaga accccggcac aggccgcgga caccatggcg agatcaagaa
    14341 cgccagttgc cgtggcgcgc acggcgccga gcgacatcat cgttgtcacc atcagcgcca
    14401 ggaccgcggt cgcggacaga ttgcggccaa cggcgaaaaa ggcggagggt ttcatgctgc
    14461 gcgccgcgaa ccctgccgcg gtcaagccgc caactgccag catcagggtc agtttcgcca
    14521 cgagttcgga gggcgcgccg agccgatccg ctaccatgct gccggagacg agcacgaaga
    14581 cgacggcgac caaaaggact gaaccagcgc tccgaaagtt cacgtcggca tccgcacgtt
    14641 gggagggttg cgctcgtgtt agctcatcgc gcatggttag gcgagacgtc tgaaaactct
    14701 gtgctcgggc gttgaaatcg cctgtagccc aaaggattgc ttgccaaacc gactggaaag
    14761 agcgcacttt gtgccttgac tggtggcaat ttggggaagc agatttcgat ggctggtggc
    14821 gcggaagcag tttctgttat cattgctgat gatcatccct tgttccgggg tgcgctccgg
    14881 ctggccgtcg acggcatgtt ttccgcgcag aaaattgtag aggcagcgag tctggacgag
    14941 gtcctgtccg cgcttcagaa gacgccggac gtcgacctgg tgctgttgga tctgcgcatg
    15001 cccggcatga gcggctattc gggcctcacc catatccgtt cgcagtatcc cggcgttccg
    15061 gtggtgatcg tttcgggcgc atccgacccg acgactatcc ggcgctgcct tgacttcggc
    15121 gcatcgggct tcatcccgaa gacgaccgac attgacagca tgcgcaaagc cattcgtgcc
    15181 gtgctcaatg gcggatcatg gacgcctgcc gacttcgatc cggcttcccc cgtcgacaag
    15241 gagacgacag aactcgtgcg ccgcctcggg agtctcacgc cgcaacagat gcgcgtcctg
    15301 atgatgctgt ccgaagggct tctgaacaag cagatcgcct acgagctgtc ggtttccgaa
    15361 gcaaccgtga aagcacatgt ctcggcaatc ctgaccaagc tcggtgtcga gagccgcacg
    15421 caagcggtga tcgctgccgc gaagatcggc gttggcgaag gcaagacggc cggagaaagc
    15481 ggcgcctgaa gcagactcag atcagtcccg gatcgatctt tgtccgtcgc tccagccagg
    15541 acgggacgga gagtgccttc gcgtgcagga agtccgggtt gtagagcttc gaagcatagc
    15601 gggtgccgga atcgcaaagc acggtgacaa tggtgtggcc cggaccgagg tgttgcgcca
    15661 ggcggattgc tccagcaacg ttcacgccgg acgagccgcc aagacagagc ccttcgttct
    15721 ctagcagatc gaagacgatt ggtatggact cctcgtccgg gatttggaaa gccacgtcaa
    15781 ccggggcttc acgcagattt gccgttatcc gtccctggcc gataccttcg gtgatcgatg
    15841 atcccgatgc tttgagctca ccgctggtgt agtagctgta gagtgcggcg ccgggcgggt
    15901 cggccaggcc gatgatgatc ttcgggttga gggccttgag acccatgccg gtaccggcca
    15961 gggtgccgcc ggtgccaacg gcgcagatga acccgtccac cctaccgtag gtctgcgcga
    16021 agatttcggt agccgtggtg gcgatatggc cgcgccggtt ggcggtgttg tcgaactggt
    16081 tcgcccagac agcgccgttt ggctcggatg cgttcaagcg ctcggccagc ctgcccgata
    16141 ccttcacgta gttgttcgga ttggcatacg gcacggcggg gacctcgacc agctcagctc
    16201 ccgcaaaccg cagcatgtct ttcttttcct ggctttgcgt ctcgggaatg acgatcacgg
    16261 tgcgatgacc cagtgcgttg ccgacaaggg caaggccgat gcccgtgttg cctgcggttc
    16321 cctcgacgat gatgccgccc ggtctcagcg cccccgtctg acgcgcatcc tgaacgatcg
    16381 ccagcgccgc cctgtccttg accgagccgc ccggattcat gaactcggcc ttcccgagta
    16441 tctcgcaacc cgtgagctcc gaagcacggt tgaggcggat gagcggggtg tgccctatgg
    16501 cctcgacaag acctttgcgt acggtcatgg aaggcctcag gcggcaggaa cggcatatgc
    16561 cgtggtggac ttgatccgct ccatggcaaa gcgcgacgtg acgttcttca gggggatcgt
    16621 ggcgatcagg cgcttgtaga agcgatcata tgcagccatg tcggagacaa ccacatgcag
    16681 gaggtagtca acgtccccag ccatgcggtg cagggacatt acctcgggca tggcggagac
    16741 gacggttgcg aaattctcca gccacggacc ggagtgatcc ccggtctcga ctgacacgaa
    16801 gaccgataat cccagtccga gcgtctcggg cgaaaccagg gcaacgcgcc cccggatgac
    16861 cccttcggct tccagccgct ggatgcgctt ccagcacggt gtctgcgaga ggccgacctt
    16921 ctccgaaatc tgcgccatcg atagggagca gtcctcctgc aggagggata gaatcttgcg
    16981 atcgatcgcg tccagcctgg caatttcaat catggaatat ccttcccaaa caggccgaaa
    17041 tctcgcccga agtcgcatca aagacacaaa ctgaaatgga cttgtagaat gtctttctag
    17101 ccggttcaag atttgcgcgg tcactcgggc actaatgcgt tctgcaaaaa atatgatgcg
    17161 ttcgtcttga agaacaaaag gaatggttct agcctctgga ggctccatcg gggaatgatc
    17221 gttcatgcca aacgaacgcc gcgcgtcaaa tgccctgcat ccggaaaccc gcctcgtgca
    17281 tgagggcgcg ctgcggtctc agttcggcga aacctcggaa gccctcttcc tgacccaggg
    17341 ccatgtctac gagtccgcgg agcagtgcga agcgcgcttt ctggggcagg aggaggggtt
    17401 catttatgcc cgcttctcca atccgacagt gcagatgttc gagcgccggg ttgctgccct
    17461 ggaaggcgcc gaggcagcac gcgccacagc caccggcatg gcagccgtta cggcagcgct
    17521 catgggagtg gtgcgggcgg gcgatcatgt ggtagcggcg cgcgccctgt tcgggtcctg
    17581 tcgatatgtc gtggaggagc acctgccgcg ttacggcgtc gcctctacgc tgatcgatgg
    17641 ccccgatctc gccgcctggc gccgggccgt tcagccgaat accaagctgt ttttcctcga
    17701 aagtcccgcg aatccgactc tggaagtcat cgacattgcc gccgttgctg cactggccag
    17761 ggaggttggg gcgaaactcg tcgtggacaa cgtcttcgcc acgccactgt ggcagaagcc
    17821 gctgacactg ggcgcggact gcgttgtcta ctccgccacc aagcatatcg atggacaggg
    17881 ccgctgcctg ggcggcgtta tcctggcatc ccggcagttc atcgacgatc acgttcatac
    17941 gttgcttcgt cagaccgggc cggccatgtc tccgttcaac gcctgggtgc ttctcaaggc
    18001 gctggagacc ctgccgctgc ggatccgccg gcagacggag acggcgggaa taatcgccga
    18061 ctggctcgcc gggcagccgc aggtccgcgg gctacgctac cccggacgac aggatcatcc
    18121 gcaaagagag ctgatcgcca ggcagatgct gggccccagc acgctgatcg ccttcgacgt
    18181 ggcgggggga aaggccgccg ctttcgcgct tcttaacagg ctgaggctga tccgcatttc
    18241 caacaacctc ggcgatgcca agagcctgat cgttcacccc gcgacgacga cccatcagcg
    18301 gttttcaccc gaacagcgtg ccgagatggg cgtgagcgat ggcatggtcc gcctgtccat
    18361 tggtcttgaa caccccgatg atctgattgc tgacctcgct caggcgctct cggcttgaac
    18421 cgcggccgct cttgggccaa ggtcggttga tgccaaggag atgcctgtcg tgaccgaaac
    18481 tgttcaaaag accggaccac gggtcgccct cttcgtgacc tgcctcgtgg acaccatgcg
    18541 cccgagcgtc ggctttgctg ctgccaagct gctggaagat gcagggtgca cagtcgaggt
    18601 tccgcgccag acgtgctgcg gccagcctgc tttcaattcg ggggatcgta atacggcgcg
    18661 tgcgttggct gagcagatga tcgccgcatt cggaacctgt gattacattg tggctccttc
    18721 cggttcttgc gccggcatgg tgcgtgccca cttcccggaa cttttcgccg gcgatccgaa
    18781 catggctccg aaagccaacg ccttcgcggc caagacccat gaactggtct cgttcctgac
    18841 cgatgtgctc ggggtcgagg cggtggaggc gcgccacgag ggggccgtga cttaccatga
    18901 cagttgctcg ggtctgcgcg agatgggcgt gaaaggccaa ccgcgcaagc tgctcgcgac
    18961 tgtgcagggc ctgacgctga cggaaatggc cgagaacgaa acctgttgtg gcttcggcgg
    19021 ctcattcgcc gtgaagtatg gcgaattgtc ggacgccatc gtctccaaga aggcagaaaa
    19081 catcgaggca tcgggcgcgc cgatgctgct cgcgggcgac ctcggatgcc tcctgaacat
    19141 ggcgggaaag ctgcagcggc gtggttcggc cgttcaggtc cgtcacatcg cggaagtgct
    19201 tgccggaacg acagatgaac cgccgatcgg ggttccccgg tcgccgctag gcgatacagg
    19261 tggcggccgt ggctgaaccg atgcgcgccc cggtctcgga aatccgtccg gatcggcgcg
    19321 ggtggcgcct ggcattgctc gccgcacttc ttctccttgc tggatccgcc atgctttggg
    19381 cgcgctatgg cagcgccgtc ttcgtggaac tgcttgccgc cgcctgggtc gcctgtttct
    19441 gagccctgcg cgaggtacgt gccagcccgt gccgtgtctt gttccagcga tggggggcct
    19501 tgaggagttc gaagaacgca cgccacgctg caacggaaac caggagctga tacagcggca
    19561 gcagggcaat ccaagggatc agcccgtgca ggcgccgtcg gtgcagcccg agaagagcgg
    19621 gcaacacggt cgatagcagg ccggcaacgc caagcacgat ggtcaccgca tccgcgaagc
    19681 ggtcgagcgg ccctccggct ccaaacggtt cctgccgcat cagacgctgg gcaagaaggg
    19741 cgtagaagat cggcgctccc aatgcgccga cgacaacgcc ggcggacatc gaaacggcgc
    19801 taaagaaatt cacaggtccc atcgcgcgga tggccgtcag cggggcgcga ctgtgaacca
    19861 ccacggtttg catccacccc ttgagccagc gcgtgcgctg ggcaagccag gggcgcaccg
    19921 tgctcggcgc ttcctctgtt gtcgtcgagg gcaggtcgtc caccagcagc cgatgctgcg
    19981 ccagccgaat gccgagatcg gcatcctccg tgacgttcca ggcgtcccag agcccgatcc
    20041 gccgcaaagc ttccacccgg aagtgattcg aggtgccgcc aagaggtacc ggaagtccaa
    20101 gtctggcgtt cccggccttc accacgtcaa acagcgccgc atactcgatg gtaaacaggc
    20161 gcgtcaggac ggagtcgaac gtgttatcta tggcgagccg cgcctgcagg caggcaacgc
    20221 gcttcggcgc ggcacgaaag gcggcggcgg cggcgcgcaa ctgatcggga tctggttcgt
    20281 cctcggcgtc gtagacgaca acatgctctc ccgtgacaaa gggcatggcg gcgttgagcg
    20341 cgcgcggttt ggtgtgcggc gctccggccg gcgcgacgag aacggaaaag gaagctggca
    20401 gcaacagccg ttcgagacgg gagcggactt caagatcatg ctcttcgatc accagcagga
    20461 cttgcagttt ctcgcgcgga tagtcgatca gattgagcgc ggcgatcagc ttttccacga
    20521 cttccgcctc gcgatacagc ggcacaagga tgctgtagcg cggcaagcgc gtatcgtttg
    20581 tctcaatcac gcgggtggcg aggggagcgc cttcaacgca agccgagagc atgacgaggc
    20641 ttgcaccgag gaacaacagg ccgagcacca ggggcagcag cgtcagcatc tcctgcggcc
    20701 acaaggcagc gcagagggca gacaggaccg cacagaaccc cagcacacgc ttctggcgca
    20761 tcacggcccc tgatcgggcc gagagtgccc cgggaacgct ttccgcagca tccagggcga
    20821 tgcgctcgga aaaatgccgg atcagaatgc tccgataggc atcaccggtt gccaaggcca
    20881 cctggccatg tcgcaaacgg ggggtggttt caagcaggac gcgcagcagc ggaccggcgc
    20941 aggagaccag caggacatcg cgaccgtcca ccagacgcgc ccggcaccac ccttgccgca
    21001 aggcatcttc gaggcgcacg cgcgggtcgg gttgggcctg ctcgatgacc ggcgcatcga
    21061 tccatctgcc gagccgttgc aagtacgttt cctcgtcgag cgctcccgag gccacaaggg
    21121 cttcccaccc ggccacgcca agtccgagcg ccctttgaga ggccctgtac agcgtcggaa
    21181 agtcgaaccc ttgcagaaac aggaaagaca gatcagccgg cagggccgag ggcgcgacga
    21241 cgtcccggcg ccgatctctt gaggatcttg ctgtccaaaa caccccttgc actggccgtg
    21301 tttcgcggcc agagtcggca ttcacgccca actgcatgcc ctaagcccca gtcttcctga
    21361 cgctttggtt gcccccagga accagataac gacatgtcag tgtttctgtc gagactgctt
    21421 gttccggtcc ttgctttctt ctgtggtttt cttggcgatg agcgccccgc ccaggccgag
    21481 gagaaagtct tcattcccgg atactgggat ctgcggcggc gcgcggaaaa gccggatgtc
    21541 tcgcgcatcc gtcagattcg cttcctgacg gaaagcgact atccgccgtt ccacttcatt
    21601 ggtccgaacg gaatgctgac cggttttgag gtcgatctcg cgcgcgcgtc ctgcgaggtg
    21661 ctgggcgtga cctgcaccat acagccccat cgctgggaca ggttgctgga cgcgctgcgc
    21721 gggaacgtgg gcgatgcggt gatcgcgatg gttgcgatca cgccggagaa ccgccgccag
    21781 ttcctgctga cgtcggccta ccatagaacg ccggcgcggt ttgtcgtgtc caggaccgcg
    21841 cccggaggcg ccctgggagc agatgcgttg aagggaaagc gcgtggcggt tgtggttggc
    21901 tcaacccatg aagcctactt gcgcgcccac tttccgacgg cggtcgttgt ggcggaaccg
    21961 aacatcgcct cggtgctgac gtccgtgcag tatggcggcg ttgcggcggc ttttgtcgac
    22021 ggcgtcagcg cggcgttctg gctcaatggc gaggcgtcgg gcaattgttg ccggttcgcc
    22081 ggcggagcct ttacggaaag ccgctacttc ggagagggcg ccgggatcgt tgtccgccgg
    22141 gactcccagc atatccagca ggcgctcgac tgggcattgc aaaggctggc atccgacgga
    22201 acctatgcca ccctttatct gaagtacttt cctgttggta tctactgact ggcaggcctt
    22261 ggtcgctccg gcgacggctt cgtcgcgcgg tcgtggaatc ccttcgcccc acttgcaatt
    22321 cggcatcgat agtttatata tgaactgttc aattcgcgat cggcatcccc ggtcgctgcg
    22381 ggagtccgac tgcggacccg ccatctgaag ggagacccga atgcccgtct acaaggcccc
    22441 ggtcgacgac gtttcgttcc tgctcaacga cgtgtttcat atagagcgct acggaaatct
    22501 gccgggattt tccgatcttt cgccggaagt tcttgaggcg atcgtgtcgg aagcggccaa
    22561 gctgtgcgaa gagcagttgc agccgctgaa ccgggtcggc gatcagcagg gctgcaagcg
    22621 ccatgatgat gggtccgtga cgacgccgaa aggcttcaag gaggcgttcc gcgcctactc
    22681 ggatggcgga tggctgggca tttccgcgcc ggagcaattc ggcggccagg gcctgcccta
    22741 taccgtcggc gcgatcatga acgagttctc ttcggccgcg aacatggcgt tctccatgta
    22801 tcccggcctg acgctgggcg cgatggccgc cattgcccag catggcagcg aggagcagaa
    22861 ggccctgttc ctgccccgga tggtcgaggg caaatggtcg ggcactatga atctgaccga
    22921 accgcattgc ggcacggacc ttggcctgct gcgcacgaag gccgtgccaa acggcgacgg
    22981 gtcctatgcc atcacgggga ccaagatatt catctcggcc ggcgagcagg acatcacgga
    23041 gaacatcatt cacctcgtgc tggcgcgcat cgagggcgcg ccggcaggcg ttaagggcat
    23101 ctcgctgttc atcgtgccga aaatgaacgt caatccggac ggttctctag ccggccgcaa
    23161 cgcggtcgtc tgcggctcga tcgaacacaa gatgggcatc cacggcaatt cgacctgcgt
    23221 gatgaactac gacggggcga agggtttcct gatcggccag gaaaacaagg gtcttgccgc
    23281 catgttcgtg atgatgaacg aggcccgcct gggtgtcggc atccagggtc tggcccagtc
    23341 cgaagctgcc tatcagaacg cggtggctta tgcgaaggac cgcctgcagg gtcgtgccct
    23401 gaccggtccc aaggagccgg ccaaggccgc tgacccgatc atcgtgcatc ccgatgtgcg
    23461 ccgcacgctg ctgaccatcc gcgccttcaa cgaggcggcc cgcgccttcg tgctgtggac
    23521 atcgctgaag agcgacgtcg cccaccgatc gggcgacgcc aaggagcagc aggccgccga
    23581 cgacatgctt ggcctgatga cgccggtgct gaagggcttc ctcacagacg ttggttttga
    23641 caacgccgtg cgtgcccagc agatgttcgg cggacacggc tacatcgagg aatggggcat
    23701 gtcgcaattc gtgcgcgatg cccgcatcgc catgatctac gaaggcgcca acggcattca
    23761 ggctctcgat ctcgtcggcc gcaagcttgg tcgcgacggc gggcgcggca tcatgacctt
    23821 cttcggtgag gtcggccagt tcctcaagga caattccgcc gatgagggga tgaagccgtt
    23881 tgtcggccca ctgcagacct ctctcggaca cctccagcag gcgacgatgt ggttcatgca
    23941 gaacgccatg gccaagccgg acaatgccgg tgccggctcc accgactaca tgcacctgat
    24001 gggcttcgtg gtcctaggat atatgtgggc tctgcaagcc aaggtggcgc aggagaagct
    24061 gaaaggcggg gcgaatgatc gcatgcaggc caagctcgcg gtcggccggt tctacatgga
    24121 gcggatgctg ccggagaccg gcatgcggct cgctcgggtg accgttggtg ccgattcagt
    24181 gatggcgctg ccggcagagg cgttttgacg agccgcgccg ccatggcgcg caagacgatg
    24241 cccctcccgg tgcggttcaa cgccgcgctg tcggaggcgg catatgcgca gttgcgggcg
    24301 ctcaatgccc gcactgggct ttccaacaat tatctgctca cgatcctact ggaacggctt
    24361 gacgccatcg ccgatccgga tcgacttcag acggaattcg acgctttcat ttcggaatat
    24421 ggcgcgcccg catccggtgt gcgcatgagc aagggatcag gaggccccaa tggctgacgc
    24481 gttcatctat gatcacgtgc gtaccccgcg cggcaagggc aaggcagacg gcagcctgca
    24541 tgaagtgact gcggtcaacc tctcgatcca gacgctgaag gcggtgcgcg agcgcaacag
    24601 cctcgacacg gcgctggtcg aggatgtggt ccttggctgt gtcgatccgg tcggcgagca
    24661 gtcggcggtc attgcccgtt ccgcggtgat cggcgcaggg tatggcaagc agactccggg
    24721 cgtgcagatc aaccggttct gcgcgtccgg cctcgatgcc gttgcgctgg cggcggcaca
    24781 ggtgatggcc gggcagaagg acctcgctat cggcggcggc gtggaatcca tgagccgcgt
    24841 cgggatgggc gcttccggca tgctgccggc ggctgatccc tccatcgcca tcccgtccta
    24901 cttctcgccg cagggcgtgg cggcggatat gatcgccacg aaattcggct tttcgcgcga
    24961 cgatctcgac gcttttgctg tcgaaagcca caagcgggct gccggggcct gggccgacgg
    25021 tcggttcgcg aagtcggtcg tgccggtgaa ggatatcaac ggcctgacca ttctcgaccg
    25081 tgacgagacg gtgcgcccgg acgccaacat gcagtcgctc ggggccctga agccggcttt
    25141 cgccatgttg ggcgaacagg caggctttga tgcggtactg atccaggcgc accccgacgt
    25201 ggagagcatc aaccacgtgc atcacgccgg caattcgtcc ggcatcgttg atggcgcttc
    25261 cgccgtgctg ctcggctcga aggctggcgg caagaaagcc ggactcaagc cacgcgcgcg
    25321 catccggggc ttcgcctcgg tcggatccga cctggcgttg atcctcaccg gtcccgtcga
    25381 tgtcaccgaa ctgctgttga agaagacgaa gatgaccttg aaggacatcg atctgttcga
    25441 aatcaacgag gccttcgcct gcgtgccgtt gcactacatg ctttcgctgg acctcgatcc
    25501 ggccaaggtc aacgtcaacg gcggtgccat cgccatgggg cacccgctcg gcgcgacggg
    25561 cggcatgatc ctgggtacgg tgatcgacga acttgagcgg caggacaagg cggtgggttt
    25621 ggttaccctc tgtgtcgccc acggcatggg cactgccatg atcgtcgagc gcgtgtaatc
    25681 gaaaccggac ggagaacagc catgaaactc accaatttcc gtttcgagac cgacgccgac
    25741 ggcattgcga cgctgttctg ggaccaggca gatcgctcga tgaacgtcat caccatcgag
    25801 tggatgcagg agttgaacaa ggcgatagac cacatcgccg cgacgcccga gatcaaaggc
    25861 tgcgtcgtcg cgtctggcaa gccagactcc ttctccggtg gcgccgatct gaccatgttg
    25921 caggcgaccg gcctgcaata cgccaagatg gcgaaggccg agggcgaaga aaaggcgatg
    25981 gccttctttt tcgagcagac gtcggccctc tcccgacttt tccgcaagct tgagacctgc
    26041 ggcaagccgt tcgcggctgc gatccacggc gtctgcatgg ggggggcgtt cgaattggcc
    26101 ctgtcctgcc atcaccgcgt cgcctccgac agtgatcgga cccgcctggg cctgccggag
    26161 atcaaggttg gcctgttccc cggcgcgggc ggcacccagc gcgtgccgcg cctgatgcag
    26221 actggcgatg ccctgcaaat gctgttcaag ggcgaccaga ttcgtcccgc cgccgcgcgc
    26281 ggcatggggc tgatccacca ggtggtcccg caggacagga tcatcgagac tgccaaggcc
    26341 tggatcaagg caggcggctc cgccaagcag ccctgggaca cggagggcta caagctcccg
    26401 tccaacaagg tgcactcgcc gcaaggcatg atgatctggc cgccggcctc tgcgatctac
    26461 cgcaaggaaa cggccgacaa ctacccggct gcccgcgcca tcctgcaggc ggtctatgaa
    26521 ggcctgcaac tgccgatgga cctcgcgctg cgcgtcgaga gtcgctactt cgcgaagatc
    26581 ctgcggtcgc cggaagcggc ggcgatgatc cgcagcctgt tcctttcgaa gggcgaactt
    26641 gagaaaggtg cgcgccgccc ggcgggtatt ccagccacga aactgatgac catcggcgtt
    26701 gtcggcgccg gcttcatggg cgcgggcatc gcctatgtca cggcgctggc cggcatgaat
    26761 gtcgtgctgg tggaccgcga ccaggaggcc gccgacaagg gcaaggcctt ctctcacaag
    26821 atcttgtccg accagatcat gaaggggcgt gccaagtccg cggatcgtga cgccctgctc
    26881 ggccgtatca acgcgaccgc tgactatgcc gcgttgaagg cgtgcgatct gatcgtcgaa
    26941 gcggttttcg aggaccgcgc ggtcaaggcc gaagtgatca agaagatcga ggcggtgatc
    27001 ggcaaggagg tgatctttgc ctccaatacc tcgacccttc cgatcacctc actggcggag
    27061 aattcggcga ggccggatca gttcatcggc attcatttct tctcgcccgt ggacaagatg
    27121 ctgctggtcg agatcatcct gggcaagaag accggtgacc gtgccctggc gacggcgctc
    27181 gactacgtcc gcgccatcaa gaagacgccg attgtcgtca atgacacccg gggcttcttc
    27241 gccaaccgct gcgtgctgaa ttacgtgcgc gaaggtcagc tgatgttcat ggaaggtgta
    27301 ccggccacga tggttgaaac cgtcgccaag atggcgggca tgcccgttgg gcccctgtcg
    27361 ctgatggatg aagtcgcgct cgatctgggc tggaagattc tccaggcgac caagaaggat
    27421 ctgggagcca aggccatcga tcccgtgcag gaggcgctgc ttgaggaaat ggtggtcaag
    27481 cgggggcgct tcggccgcaa gaaccgcatg ggcttctatg actatcccga gaagggaccc
    27541 aagaagctct ggccgggcct cgctgacgtg caaaagaaga agttggcgcc ggcaaccatc
    27601 gatatcgccg aactcaagca gcggatgctc gtggtacagg ctctggaagc ggcgcggact
    27661 gtcgaagagg gtgtcatcac ggacgtgcgc gaagcggatg ttggctcgat cctggggttt
    27721 ggctttgcgc cttacaccgg cggcacgctg tcctacatcg acggaatggg cacgaaggcc
    27781 ttcgtggcgc tgtgcgacaa gctggcccgc aagcatggcg cacgcttcaa gccgaacaaa
    27841 ctgctgagag acatggccga gaaaggcgag acgttctacg gccggttcgc cccggtgcga
    27901 aagcaggcgg cgtgaccgtc aggagtgctc cgacgccatg aatctctggc ttcgcatgct
    27961 ctggttggcg ttgacggcgc ggtttcggcc gcgtttcgat acgcctctcg gtctatcgcg
    28021 cctgacgctg cgggtctggc ccttcgacgt ggacgtcaac cgccacctca acaacgggcg
    28081 ctatctcacc atcgccgatc tggggcgggc ggatctgttt catcgatccg gcgtatggcg
    28141 gcaggcgcgc gctatgggcc tcttctccac aatgctgggc gttgccatcc gcttccagaa
    28201 agagatcaag ccgctgcgga ccttcgacct tgaatcgcgc tgggtctact ggaacgagcg
    28261 ggtcgcggtg atggagcacc tgttcctgat cggggcagga gagggccggc gtgttgctgc
    28321 ccgcgagttc gcgtcgtgcg gcttctacga catgaaggtg cgcaggtttg tcgatccggg
    28381 tgatctgttc cgcaaggctg gagcgcaagg cggttcgccg ccggcgagcg aggagatcct
    28441 ggctttcctg aaggcgcagg aaaccctgaa gcaggtgtga ttcaattctg gggagaacta
    28501 cttccccagg agccgcgcga gcttcttcag cctgttttct tcgctctcag tggcatcgaa
    28561 cgtgccccag agctgtgcct tgccgtcgaa cagatagatc gtggcggtgt ggtccatcgt
    28621 gtagcccgac ggggtcggta ccttgcgata gaaggcacgg tgggcgcgcg cgacggcggc
    28681 gatctcttcc gccgtgccgc tcatgccgat gatgcgattg tcgaaggccg agatgtagct
    28741 cgacaacagc tccggtgtgt cacgctccgg gtcgacggtg acaaaaaaca cccggaagtc
    28801 ctttgccatc ggtccggact tgcgcaggat ctcggacagt tcccacatgg cggtggggca
    28861 gatgtccgga caatgggtga agccgaagaa aacgccgtaa ggcctgccgc gcatcacctc
    28921 tgcggtgttg aggcgcttgc cgtccatggc tgtcagcgtg aaggggccgc caatggcaac
    28981 gcccgccggc ttttccgacg acagcgtgac atacagcatg accgccgaaa gtgcggcgat
    29041 cagtccccag gcaacaacgc ggacgattcg taggggattg agcgggtcgg gcatgggtca
    29101 cctcgggttc gaggtcaggc ccggtcgagc gcgaccgggc ctgatccttc gtgtcagtgc
    29161 gcgtggccgc cgcttgtgga gccgatcggc cggacgatgt attcgacggg gattttaccg
    29221 gccttttcga acaccagttc gcccttcagc ttctcgccct ccttcaactg ccgtttgagg
    29281 tcgatgaaca tgacatggta gccaccgggc ttcagttccg cctttccgcc ggctggcacc
    29341 tccaggccct tggggagggc gcgcattttc atcacgccgc cctccatggc catctcatgc
    29401 acttcgaaga cattcgcaac ttcgaaggag ccgccgatga ggcggtccgc ggtcccagtc
    29461 ttgttctcga tggtcatgaa ccccccggcg accttggcac ccggcggtgt ggcacgtgtc
    29521 cagggcagat ggagaatcag accggacgcc gttggcgccg cgtggtgctg gtgctgcgct
    29581 tgggtctggg tgccgtgact accgtgaccg ccagcctgcg cgacctgctt cgctttgatg
    29641 cggacagcgg gcgcagggct cttcagggag tgcggatcaa cgccaggcgc ggcgacttcc
    29701 gaccaacgat gttcgccctt ctcgcacaat tgtgtgaccg ggaagtgcag cggacccgtg
    29761 ggaacagcgc ccgaaatccg gcttgaaaac acgaattcgt cgtattctga atccttcagc
    29821 tgcccatcgg accacacgat ttcctttacg ccttccgaca tggggcggcc gaagtagtca
    29881 taggtgcggg cataggcgcc ggtggtcgtc ttgagcttcc agcccgactt cggcatcggc
    29941 ttgacgccaa tgacgccctc cggaacggag atgatgacgg tatgcgtggg ggagccttcg
    30001 catccgtgcc cgatcttgac gacgcccttg aaggaactgt cctgtgtcgc ttcggcgatg
    30061 tcgaacgttg cgtgggcatg ggctgtcgct gtcaggcaaa gcgcagcaac gctgatggcg
    30121 gccatgcggc cgatggtcgt gaacatggtt ttcctcgatg tctgaactgg tttccaaagc
    30181 atgcggaact cagatcaggg caggaggtcc ccgcgcgtcg aaaagctgga cttcaaagac
    30241 gacgacagca gcctcggatg atggatcggg tgccgcccgt gtcgcgaaac gcttcgtttc
    30301 cggaggccgc tcgccaccgc aatcggaggc tgcaaaggcg cacagcacgc agtgctggtg
    30361 catggccggt gacttgccgg gcacctccgg gttggtgtca tcggaaaggc agagaacacc
    30421 cgaaaaccca gctctggcgc caggatgcgc gccttgcaga cgtgctgcgg ccaccgcggc
    30481 aatgaacacc acggatgaca gggccagcgc gatgatctcg cgcgtgagcg aacgccgtcg
    30541 aggcctgaac atccgtcttc ttaagcgcgc tgcatgccgc aaggcaagcc ggactactcc
    30601 gccgcgacct cttgtgccgg ccgataggtg acgaggatgg cccgaagcgc tgcaggcttc
    30661 agcggcttgt tgagcacctg catccccatc tcggcggcgc gatcccgaac ctcggtcgag
    30721 cggtctgcgg ttatcagaac cgccgggaga tcgatccgcg catgcttgcg gaggcggcgc
    30781 accagatcga ggccgtcact gttgtcgagg tggtaatccg cgaggatgac gtccggattg
    30841 cctgccaggc gcaaggtccg cttggcatcg tcgaaagatg cggccgtcgc cacgacgcac
    30901 ccccagccct caaggagcgt gcgtagcccg tcgaggatgg taggatcgtt gtcgatcgcc
    30961 agcacatgca agcccgcaag cggttgttgt gaggcattcg tgacctccgg cggaacaaag
    31021 gtcaccggtt cggcgctcgc gatgggcgcc tctagtgaaa aggtcgatcc ctttcccgct
    31081 gtcgaggtaa cggcaatgcg atgattgagt acacgggcga tgcgctctac gatcgacagg
    31141 ccaagtccga gaccgcgcgc gacccgggct ccctggtcga gtcgctggaa ttccttgaaa
    31201 atgatgcgct gcttcgatgc cgggataccg atgccagtgt cgcggacctc aatgcgcacc
    31261 atgcccttcc gccgccggca gccaaccaga acgcggccct tctcggtgta cttgatggcg
    31321 ttggagataa ggttttgcag cgcgcggcgc atcaggcggc gatcggagcg aatggaggcg
    31381 cccgacggta caaaggtcag ttccagcccc ttggctcgga cggctggatc gaattcaacg
    31441 gcgagctggg caaacaagtc gtcgatccgg aagacgctcc actccggcct cagcttcccg
    31501 gtgtcgagcc gcgagatctc cagaagtgcg ccaaggattt cctcgacggc gtccagtgaa
    31561 gccccgacgt tctgggcaag ttgggcgtgc ggggtggcgt cggtacgctc aaccaaggag
    31621 gtcgcgtaga ggcgggcggc attgagcggc tgcaggatgt catgactggc ggctgccaga
    31681 aaccgggtct tggagaggtt tgcgtcatcc gcctcggcct tggccctggc cagttcttca
    31741 ttgacgcgga ccaattcgtc agtgcgctca aggacacgcc gttccagcga ttcgtttgcc
    31801 tgtgacagcg cttcctcggc ctcgaccgct tcggtgatgt ccgtataggt cgtgacaatg
    31861 ccgccgtccg ggagatggtt ggatcggatc tcgagcacct tgcccagagg gtgcaagcgc
    31921 agacgcaccg gctcggtatt gtgcacgaag ctctctaggc gcgatgcaat gaagtccggt
    31981 gaactggagg gcccgtagat gccgcgacgt gcattgtacc caatgatgtc gtccagcccc
    32041 acgccgaaac gcaccagttc gtgcggcaac tcgaacaggt cctggaaggc acggttccaa
    32101 cagaccaggc gcaggtcgct gtcgaacacg gtgacgcctt gcttggcatg gtcgagggcg
    32161 tgctgaagca gggtccgact gtactggatc gcagcagaag cgtcgtccag cagcttgagc
    32221 gcggcttcgg tcgaaacgtt gcggcgcttc agcatgagcg acaggacgag gcgggaagaa
    32281 gctgccccga tcaccgaagc aagcaggtgc tcggcaaagc ggatcgtatg ggcgtcggcc
    32341 tcgccgttga cgttttccgg ttgaccatgc cgcgtcagaa attcggtaaa ctcgcgggcg
    32401 gtacgttcgt ctccgaggta gcgggccacg gtctggcgca gttcggcgac cgttaccgcg
    32461 ctgcgccaca ggcggaagct cggcccggac aatccggtgt cgacgccgat gaagacattg
    32521 gcttgaaggc gctcaatcgg cgtcgcgtgc cgcgtcagcg aaaagccgac aaaagcaatg
    32581 atgttgagcg acaggctgat gatgacgcca ttcaccagcg gcgtcagacc gaagacgttg
    32641 aacgcctgcg gcctgagcca gtcgatgccg aaggggccgt ccgcgacaaa ggcggcgacg
    32701 taggagttcc cggccgcgaa ggatggcagg aaaaggagat aggcccagca cagaattccg
    32761 gcaatcagac ccgccaccgc gccacgcgca gtgccgcgat gccagaggaa tccaccgatg
    32821 aaagccggcg caagttgcga agcggcggcg aaggacagca ggcctatgct ggcgagggcc
    32881 gcttcgttcg atgcccggta gtagaggtag ccaagcagca ggatcacgac gatagccgcg
    32941 cgccggatga tcaacaccgt gccgctcatg tccgcggcag acgccggttc tggctcgccg
    33001 gccatgcgcg cgtccactgc ggttgctccg cgctcgcggt gccggctgga acggcgcaag
    33061 aggaacggca ggatcaggtc attcgaaacc atgatcccga gcgccacggt ttccacgatc
    33121 accatggccg tggccgccga aagcgcgccg accacggcga ctgcggcgac gaggttggaa
    33181 ttcgcgtaca atggcaatgc catcacggtc agatcgtggt cgacgcgccc gggaaagatg
    33241 agcatgccgg cggcggcgat aggaataacg aacaggttga tggcgacgag atagagcggg
    33301 aacagccaag cggccttctt cagatcgccg agctcccggt tctcaaccac gatcacatgg
    33361 aattgccggg gcagcagcac gaaggcaagg gccgcgatca gcgttgtgac aagccagctc
    33421 gccgcatcgg gcgcaaacgt cagcgcgcgc atgatatccg gattctggcg cgccttctcg
    33481 actagatcgc cggcgccgcc gaacattccc cagaccacga acgcgccgac gccgataaag
    33541 gccaccagtt tgaccacgga ctcggtcgct accgccagca tcagtccgct ctgatgttcg
    33601 gtcgcatcga tatgccgagt gccaaaggcg acggcgaaga aggccaggat caacgcgatc
    33661 caaagtcctc cgtccccgaa tggaagcgct ccgagctcgg gcggtcggaa ctggattccc
    33721 ggcagcgaga taaggtcaag cgacagcgcc accgctttga gctgcagcgc gatgtaggga
    33781 accatgccga caacgcagat cagcgccacg agcgctgcga cccgctcatc cttgccgtaa
    33841 cgcgtggcga tcatgtccgc gacggaagtg gtgttctgcg tcttgccgag cttcacgacc
    33901 cgggccagca gggaccagcc gaaggcaatg accagaacag gtccgatata gatcggtagg
    33961 aaatcgtagc ccgaacgcgc cgccaggccg accgagccga ggaaggtcca ggaggtgcaa
    34021 tagaccgcga gcgtcagtgc atagatgacc gggcggagcc tgccggcgag aattcgactg
    34081 ccggccgtgt cgccgaaatg cgcgacggcg aacaggatac agagatagaa cagtccggaa
    34141 aggacgatga gccagccgtc aaccatcaag cgccaggatg tagagcaagg acctttgtcc
    34201 ttgtcgtctc tagaaaaccg cgatttcgct ccgggcacaa gcgccgcttg cgccaacagg
    34261 cgccgacatc gccaggaacg gcctgcaaag cttgcaagaa tgccctgttc cgatagtctg
    34321 gccttcgtca agtcgttcta aatggatatg ctcggcagga ggggacaatc atgctgaagg
    34381 aattcaagga atttgccatg aagggcaatg tcgtcgatct ggcgatcggc gtcattattg
    34441 gcgcggcctt cgggaaaatc gtggaatcga cggtcgcgga catcatcatg ccgatcattg
    34501 gcatgctcgg aaatttcgat ttctcgaacc tctatgcctc cctcggaccg aaggtcccgg
    34561 ccggaacggg ctatgccgac gccaagaagc tggggccggt tctgggctac ggccagttca
    34621 tcacctacgt cgtgaatttc gtcattattg cctgggtgct gttcatggtc gtcaagggca
    34681 tgaacaggct caagaagccg gaagaggccg cgcctgcggc gccggcggaa ccgccggcgg
    34741 atgtcaagct gttgacggag atacgcgacc ttctcgccaa gaagtagggc atcgccggtc
    34801 ttgccttgac aggccgagcg ccggcgttga tcgctggcgc tcgttgcttt tgtgcgttcc
    34861 gttccagtag ttcatggttg cctttttccg gcgaggccct gacagtccgc ccatgaatca
    34921 gcatcacgct ctgtcttctc ccgcatcgcg ttggcgcatt ggtgaggccg cgcaggcggc
    34981 accgaccagc ggcatcgtcg aagtgttcaa ctatggccgc aaccggcctg gcctgctccc
    35041 gctgtgggtg ggtgagggcg accggaaaac accggatttc atcgttgagg cggcacagaa
    35101 gtcactgcgc gacggggaaa ccttctatac ctatcaacgg ggcattcccg ccctccgcga
    35161 agccatcgcg gcctacatgt ccgacctcta tggacaatcc ctcggagcgg accgcttttt
    35221 cgtcaccatc ggcggcatgc acgcgcttca gatcgccatg cgcatcgtcg ccggcgcggg
    35281 ggacgaggtg atcgtgccga cgccggcttg gccgaacttc atcggcgcca ttgtcgccaa
    35341 cggcagcatc agtcgccccg tcgagatgcc gcgcacggcc acgggcttcg tgctggactt
    35401 cgaggcgctg gagcaggcca tcggaccgcg gacccgggcg atctgcctca actcgcccgc
    35461 caacccgacg ggctggacag ccacgcgtgc cgatcaggag cggctgctgg atatcgcccg
    35521 tcgccatgac ctctggatta tcgccgacga gatttacgcg cgctttttct atcaggggtt
    35581 ccgggcgccg tcctttcacg acatcatggc ggaagacgac cgcattctgt ttgtccagac
    35641 gctctccaag aactgggcga tgacgggctg gcgcgtcggc tggatcgagg cgccgccggc
    35701 ccttggacag gtgatcgaga acctgatcca gtactcctcg tccggcgttg ctgtgccgct
    35761 gcagcgcggg gctgccgctg cgattgccca aggcgggtca ctcattgccg aacaggtcga
    35821 gcttgcccgc gcaaaccgga cgttgctgct ggatacactg agacgcaaca acgcgctggt
    35881 cattccggaa ccaactggcg ccttctatct gttcttcggc atcgaaggcc tgttgaacag
    35941 cacacaggcg gctttccggc tgatcgacga tgcgaacctc ggccttgcgc ccggagcgtc
    36001 ttttggaccg ggcggagaag gcttcatgcg cctgtgctac ctgcggcgag aggacgatat
    36061 tgccgaggcc gcgcgtcggc tggtcaacaa cctcgagcgg atacgcagcg gcgtctccgc
    36121 atgattgacc ggcgcgggcc gatccttgcg accgtgcaga cgttggccgc gggcatcgcc
    36181 ggtggctggg tattctcgat actcggactg ccggttccct ggctcgccgg tcctatggcg
    36241 gcggtcgcgg cgctcgcgat gtcgggacaa tccgtgcgct tgccgcgcct tttgtgtgac
    36301 accggcctgt ttgttgcggg gatctccctg ggcagcacgg tgtcgccgga agcgctggcc
    36361 gccgtttccc gctaccccgc cagcatcgtt gccctcgtcc tgtccgtcgc ggcgatcatg
    36421 gtcgtttcca tggtcctgct ggaactggcg ttcggctggg atcgtcagac cgcgtatctt
    36481 gcttccgttc ccggggcact gaccatgacc atggccctgg tggcagagac cagggcggac
    36541 gccggccgcg tggctgttgt gcaggccatc cgcctgttcg cgctggttgc gttgctgccc
    36601 atcctgataa ccggctctgc ggcctctgca ccgacgtccg gtcagaccgt catgacaccg
    36661 gccggaatgg cctgcctgtg cctggtggcg ctggcgctgg acgccctgct gacgcgaatc
    36721 ggtgcaatta cgccactctt tctcggcggc atgatctcgg cgaccgtcct gcatgtctcc
    36781 gatctggttc ccggcgcctt gccggagccg atcgtgatcg tcgggttcct tttgatcggc
    36841 gtgtatgcgg gcctgcggtt cgatggcatg acggtcgcag tgatggtccg cgtgttggtt
    36901 cccgggcttg tatcccttgc cgcgacactc gccgtgacgg tcgccgtggc ctggcttgtg
    36961 gttgggctag ccggcgtggc gcctgccgag gctctcgtcg cgttagcgcc gggtggggtc
    37021 gaagcgatga tcctgattgg agcttcgctt ggcctcgaca cgctctacat cggaacgcac
    37081 cacgtggtgc gcctgctggt gctgaacgcg ctgacgccgg tcttcgtgcc gcgacatccg
    37141 cgttcagaaa ccgagcatcg gcctcacagc gcgaatccgc cgtccgcgag atgaatctgg
    37201 cctgtggtgt agctggcttc gtcagacgcg agatagaccg ccagcatggc gatctcttcc
    37261 gctgttccga gccgccccat gggctgccgg tctatgaagg cctgccgcac ggcctttgcc
    37321 gtctggcctg tcgacttggc cagggcggcg atacggtcat ccagcgaggg cgactggatt
    37381 gtgccggggc atatggcgtt cgcccggatg cccttcttga tgaaatcagc cgccaccgct
    37441 ttggtaaggc cgatgacggc cgccttcgtc gtgccgtaga cgtaacggtt ggggatgccg
    37501 cgaaccgatg aggcaccgga cgcgatattg acgatcgatc cgcttccctt cgccagcatg
    37561 ccgggcagcc aggcggttat tgtgcgatgc atcgacgtca cattgagatc gaaggagaag
    37621 tcccaatcgt cttccgagca gtccaggacc gtaccgtggt gcacgaaccc ggcggcgttg
    37681 acgagaatat cgaccggccc ggtcttttcg gccagcatac gtacggcgcg gctcgaacgc
    37741 acgtcgagct tgcgcttttt gacgcgcgcc agtccttcca gcttcgacac ttcgacgtcg
    37801 gtggcgtaga cctttgcgcc ttcagcggcg aaagcttctg caatggcgcg gcctatcccc
    37861 tgtcccgccg ccgtcaccac cgcgaccttg cccttcaatc ggcctgccat gcgaatctct
    37921 cctgttccat cagaccggaa agttcgcgaa acgtcgggcg ctgcgcaagg cgaaacttct
    37981 ggccggaacg ccgatgggat cgtccctgtt caatgcgaca ttggcaagcc acgcttgatt
    38041 ctgcgtccgt catgagagac agttatgctc tcatgttgag ccggttggaa aaaaatacct
    38101 attgtaggtc gaggaagcga gaaaaatgac taagatgacg ccagagaaga aggcgaaact
    38161 ccgctcgagt tcaaagctcg tgcacctcgg gcgcgatgct gccgaacagt tcggatatgt
    38221 gaatacgccg atctatcgtg ggtcgaccgt tcttcacccg acggttgaag cgctgaaggg
    38281 acgcacccag cgcttcagtt acggcacgcg aggcacgcca acgacagaag cgctcgaatc
    38341 cgcctgggcc gatatcagcg gtgcggccgg gaccgtgctg gtgccgtccg ggctggctgc
    38401 ggtggtgctc gcgctgatgg cggtggtcaa ggccggcgat cacattctcg tggtggacag
    38461 tgtgtacagg ccgacccgcg agttctgcgg ctcgatgctc gccaggtatg gagtcgagac
    38521 cgaatactac gatcctgcca tcggcgcgcg gatcgctgat ctgatccggc cgaacacgtc
    38581 ccttgtgttc acggaagctc cgggttcgca gtcattcgag atgcaggaca ttccggcgat
    38641 tgccgcagcg gcgcgggctc gcggatgctg cgtgatcatg gacaacacct gggcaacgcc
    38701 gctgttcttc ccgccgcacg aacgcggtgt cgacatcgcc attgaggcgg gcacgaagta
    38761 tctgggcggg cactcggaca ttctgctcgg cctggtgacg gcgaatgcgc agtggctgcc
    38821 gaagatcaag gatgtgttca tcgcaacggc gatgtgcccc ggcccggaag acgtgtttct
    38881 cgcgttgcgg ggactgcggt ccatggaact ccgcctgaaa catgccgaac agcaggctct
    38941 gggcctggcg cgctggctgg agcagcggag cgaggtggcg cgcgttctgc atccggcgct
    39001 cgagagccac cccggtcatg ccatctggaa gcgcgatttc ctcggctcgt ccggactgtt
    39061 ctccatcatc ttgaagccgt gttcggaaaa ggctgtggcg gcaatgctcg atgggctgga
    39121 acttttcggc atgggctact cctggggtgg cttcgagagc ctggtaattc cgttcgattg
    39181 cgcgtcctat cgaaccgcaa cgcagtggac tcccggtgga ccggccctgc gcatccaggt
    39241 tgggttcgag catctcgatg atctgaaagc cgatctggaa gccggattcg aaaggctgcg
    39301 gaaaaccgga taggctgaga tcggagaacg ctgggcaagg caccaaaccc ccatgcactg
    39361 gtatcttacg cacccgcagg tccgcatcga tcccgccatc ccggttcccg actgggggct
    39421 gtccgatgtt ggtcgcgccc gcatcatgac gttggcgggc gcgccgtgga tccgtcgctt
    39481 tggccggatt gtgtccagcg gggaacgcaa ggcgatggaa agcgcccagg cggtcggctt
    39541 ggcgatcggc gttccggttg agttagccga agcctcgcat gagaacgaca gatcctccac
    39601 cggattcctg ccgccgcagg agttcgaacg catggcggat gccttcttcg cctcccctca
    39661 ggacagcgtg cgtggttggg aacgcgccat cgatgctcag gcgcgcatcg ttgccgtggt
    39721 ccgccaggtg ctccaggcac ggcccagaac gccgacccta tttgttggcc acggtggcgt
    39781 cggcacgttg ctgaagtgct ggctcagggg cagggacatc acgcgcgccg aggatcaggg
    39841 gccgggtggg ggcggcaatc actttggctt tacgctctcg cccaagggcg ttctgcacga
    39901 ttggctgccg ctggaaacgc cgccgcccgg gtgagatcgc ctgcgggtct gcggatttcg
    39961 gttgctcggc cgtcgcggcg accgtaataa acggcccgca cggaggcgca ggtgaaccgg
    40021 ttcgaacgtc ctgaaaagct cgggcacgaa gccgagatcg agtatctcga cggcgatttc
    40081 cgaattgttc ggcacggcag tttcgtacgc tgcgccgtta ccggccagcc gattgcgttg
    40141 gacgatctgc gctactggag cgtcgaatgg caggagccct acgcttccgc cgagatttcg
    40201 ctcaaacgcg tcctggctgg cggccccgcc acgctgacaa gagcgtcgat cagggccaag
    40261 ggcaaccgca gggacaccgg aaggaccgtg gtctccgccc gaagcgcttc aagcgccggt
    40321 gagccggaca gtcgggccgc atctttctcg gtcgtgacca gcatgagccc ctgagcgcgt
    40381 gcttcgtcga ggagcgactg cgcgtcgtga tccgtgaaag catggtggtc cgggaacgac
    40441 cgggcagcga cgactttggc gtagaggccg cgcaacgtct ggtgaaattt ctctggattg
    40501 ccgatgccgg cataggccag gactttctgt ccgccgaact gttgggcgat cgcgtcgtcg
    40561 accaggaatt cggttctgta caccggcttt ccggccgcct ttcccaactc ggccgcctca
    40621 gccatgccgt ggccgagtcc aatgaccagt atggcgtcca ctttgccgac ctgtgccgcg
    40681 agcggggcgc gaagcgggcc tgccggtatg cacaggccgt tccccgagcc gcgcgatccg
    40741 tccaccacgg caatggtgaa gtctttttcg agcgtcggat tttgcaggcc gtcatccatg
    40801 atcaatacgc tcgcgcccag cgcggtcgct gcctgcgcgc ccgcgacccg gtcaaccgct
    40861 gtgatcgttg atgcagcgcg cgccagcagc aaagcctcgt cgccgacctc ggacgccttg
    40921 tgaaccgccg gatcgacggc aagcggcccg ccacgatgcg cgccgcgata gccgcgcgtc
    40981 aggaaggccg gcgtttgtcc ggaagcctgc aaggcccgtg caaccgcaag tgcggtgggc
    41041 gttttcccag cgccaccgac aacgaagttg ccgatgcaga tcacgggaac gaccgcccgg
    41101 gctcctgact gctccatgcg tcgcgcggtc agccacccgt agatccagcc gatgccggcc
    41161 agcagtcgcg cgatgacggc gggcgggcgt gtccaccaga agcgcggcgc cttcacgtgg
    41221 tccggctccg cagatgggtc agatgcggtt cgagcgcgtt gagcgtgcgg ccgaccgctc
    41281 cgccgagtgc ggtggccgtc agcatggctt gtgcgcccat gcgatcgagc gtacccatcg
    41341 aatccagccg tcgggcgacc gccggcccga gttgctccgg ttcggtcacg aggtcggtgg
    41401 cgccggattc gccgaacgcg gcatagacat ccgtgaagtt gaagacgtgc ggtccgtgca
    41461 ggatggcgag tcccagcttc gccggctcga tcgggttctg cccgccgtgc tcgatcagcg
    41521 agccaccgag ataggccacc cgtccgagcc gatagatcag cccaagctcg ccgattgtgt
    41581 ctgcgacata gaggtctgtc ttgggcgtga tcgcctctcc ccgtgaccgt cgtgccgtgg
    41641 acaggccggc cctgctggct gatgtctcaa ggtgcggccc gcgttcagga tgtcgcggcg
    41701 ctacaatggt caggagcgcg ggcaggtgat cgctcgcact gatatgcgct gcaatcaccg
    41761 cctcctcctc gcccggatgt gtgctggcgg cgatgaacac cggacgctcg ccgatctggc
    41821 cacgcaggga tcgtagcgtg gcggggtcgg caggcggggc ggcgacatcg aacttgatgt
    41881 tgccgaccga acacgcgcgg gtagcgccga gcaatcgata gcgctccgca tcggcctcgc
    41941 tctgtgccag cacaagcgcg aaccggtgca acaaggcgcc aatgaagcgc ggcgccttgc
    42001 gccaggtgcg aaaggagcgt ggcgacatgc ggccgttgac aatgccaaga accgtaccgg
    42061 atgccgacgc gatccggatc agattgggcc agagttcgga ttccgcaaaa agtgccaggt
    42121 ccggttgcca gtgagtgatg aaccgctctg cggcggttgg cgtgtcgact ggcgcgaact
    42181 ggtgcaaagc gcctgccggc aaccgccttg cggcaacttc ggccgaggtt accgtgccgg
    42241 acgtaagcag gacgttgaac cttttcgcca ccagcgcgtc gatcaaaggc aggagcgaca
    42301 cggtctcgcc gacgctggcg ccatgcatcc agaccagcgg tccatccggg cgcttcaggg
    42361 tggtgagacc aaggcgttcg ggcatgcgtt ctgcatgctc cttcccgcgc ttctgacggc
    42421 tgcgcaggaa ggccggcatc gccggttcga tcaccgtcgt ggccaggcga tgcagatgca
    42481 gcgacagcgg gtcgcggccg gacatgtcag cgtacggctt ccgctgccgc gacggcccgt
    42541 gcctcgatga cactggcgcg gccggctccc ggatccttcg atccaaccag tgcataggcc
    42601 acggcgtgaa cacggtcgag ctcggcctcg acagcaaggc gcgcggtctc cagttccgct
    42661 tcgctggctt cctctgccac atgaaccggt tgcccgacgg caatgccgca cttgccgaaa
    42721 ggcaagccga tggagcttct gtcccaggac ttcaggtcta tgcgacggct gcagacgacg
    42781 gcgagcggat agatcgggcg gccggatttc tgtgccagcg tgacgatacc cttgccgcag
    42841 acacgcgaaa cctttggaac atcggctgta agcgccatgt tttcgccgcg ctcaagaacc
    42901 gagatcatgt cgaacatcgc cttgattcca ccgcgcttgc gcacctgatc gccgcgctgg
    42961 gctccggaac cgcgcaccag accgatgccc agcttgtggg ccgcgatggc attcatttcg
    43021 ccgtcaccgt ggcgggagat cagcacgctg atcctgtggt gcggcttgcg gaagaacggg
    43081 atcatgaagt gctggccatg ccacatggcg acaatcaccg gctgatccgc ctcgatgccg
    43141 gcataggcat ccggcggatc gaagtgcatg ctgttggtcc ggcgcacaag agtcagataa
    43201 ccggccagaa gcgaacccat cgcttcctgc accggccttg agcgccagat ctctttcagc
    43261 atgggccagc tttcagtgcg cgtctgtcga cgggtcgagc aaccggtgca gatgcaccac
    43321 gaagtagcgc atctgggcgt tgtcgacggt ttgctgtgcc ttggccttcc aggccgcatg
    43381 ggccgacttg tagtccggga aaaggccaac cacgtccact ttggacaggt ccttgaactc
    43441 cgaggagtcg aggctcgtga gctcgcctcc gagcacgagg tgaagcagtt gcttgtcggg
    43501 catcatctat tccgtattct gttacgtcat catggccgtt caggctggca tccgctcaag
    43561 aagcgccaac aggcgctcca cgagcgcggg cggagcagcg acgagggctg gatgcaccgg
    43621 atcgggagcg ttgtaaactg gccgttcgcc ggacgcgctc aacaggacgc agccggtttc
    43681 cgccaaaatc acgtcggctg cggcgatatc ccaatcatgc gcgcccttgc tcgccagcgc
    43741 cgcagtgatg cctcccgaag ctaccgcgac gagccggtag gccagagccc ggagtggttc
    43801 cgatgcggaa agttcgggat cggcggcgac aatgcgctct atcaggggac gcgggccagc
    43861 ggtccggatc ggtcggtcct gttggcctgc gatgattgga aggcgtgcac cgttcaggaa
    43921 cgcgccgctt ccggacgaag cctcaaaggt ttcattccgg gctggcgcgt gaacgacgcc
    43981 ggcaacgggc ctcccgtcct ccatcatcgc gatggaaacg caccactcgt cgcgcccctg
    44041 catgaaggcg cgcgtaccgt cgatcgggtc gacgacgaac acacggcggc tatgcaaccg
    44101 ctgcaactca tgtgccgcct cctcggagaa cacggggacg tcgggggccg aggctgacag
    44161 caccgcgcga atggccgcgt cagcgtcatg gtcggcctcc gtcaccggcg agccgccctc
    44221 cttccagctt acgcgggccg tggtgtttga gcccgccctg aaatgacgca gcgccgcttc
    44281 gctggcggcc tgggccgcgc ggcgcatgag accaagatac ggggtcgggg aagctgtcgc
    44341 catcattcca attgcttcaa gactgcctgc acgccaatgg caagccctat gtcaagcggc
    44401 ggctcgatgc tgaatgaagc tgcggcaaac atcatgtgcg cgataaccct ccatcaagca
    44461 tcgtgcgcat ttcaagccgc atctgcgctc tgtacgccct cttaaccagc aaaattaagc
    44521 gcttcggagc ggttctcccg gaaagtcggt tcgcaacggg gaacacagag tccccggcag
    44581 ggaaaaggca tcgaacatga caggcccatt cgagggcctc tccccggcgc gattcgcgtt
    44641 ggccggggag ggcgcccgac cgcccagacc ggcgaatgac aaccagccag gcacgaaggg
    44701 cgcccaggga ttccatgggc tgctcctgcg ccgagatggc gaaaaatacg agatcctgct
    44761 ctgccatcgc gagcccggcc tgtcgcaacg ggtcgaaaca gtcgacgatg acgtggatgt
    44821 catagccttg tggcgaggca tcgggcgcaa gctgaacctg ccgctgttcg ccgagacggc
    44881 cgatggggag atcgtgcaga tggagccgat gccctgcctc gtggcagcgc cgcgccgtta
    44941 cgggtcgccg ctgtccggtc ggcggccccg ctttcttgcc tgtcggaaaa tgggcgcacc
    45001 ccggcccgat gccaagactc gcgccggcgc tcaaagagcc ctgtaggcag attccagcac
    45061 aagcccggcg gtcaggatca ggccggaatc tcggttggac ttgaacagac ggagtgccag
    45121 cagccaatcg cccagcctga gcgcgcgcac ctgccagagc aggtgggcgg caaacaggcc
    45181 gaggaaaaac caggcgatgg gcgcgccatc cggcagcgcg taccacagcg ccgcgccgac
    45241 aaggccgacg gtcagcgcgt acatcccggc cacgagggcc ggcgcgcgcg gtccccaggc
    45301 aatggccgtg gagccgatgc cgactaagga atcatcctcg acatcctgca gggcgtaaat
    45361 ggtgtcgtag cccaccgtcc aggtgatcgc cgctgcgtag agcaggagcg ccggtgcttc
    45421 gagcttgccg aaaatcgccg cccagcccat caagccgccc caggcgaagg cgaggcccag
    45481 cacaatttgc ggatggttgg tgacgcgttt catgaacgga tagacgacca cgggcaggag
    45541 cgcggcaaaa ccgagcataa cggtgaagtt gttgaacgtg agcagcacgg caagcccggc
    45601 aaggcatagg gccagcatga aggccgccgc ctggcgcgcg gaaacggcgc ctgaagggat
    45661 tggtcggccg cgcgttcggg ccaccctccc atccaggtca cggtcgacaa tgtcgttcca
    45721 cgtcgaccct gcgccgcgca tcacgatggc tccggcaaga aacagcagga agtgcccgac
    45781 atgcggccat tggccggcgg cggcgctcgc cagg
//
LOCUS       DAOMXN010000006        45147 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_594_length_45147_cov_12.527655, whole genome shotgun sequence.
ACCESSION   DAOMXN010000006 DAOMXN010000000
VERSION     DAOMXN010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 45147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45147
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_594_length_45147_cov_12.527655"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="PK812_01385"
     CDS             <1..726
                     /locus_tag="PK812_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004687343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ68233.1"
                     /translation="VSALAAIAARALLREQGLEVAADEQKARQIDVVVQRRYNPEGIS
                     QYNIVPGLLGVILQMTMVMMTAMALTREIERGTMENLLAMPATATEIMLGKVLPFLIV
                     GGVQMAVVLVAAKVLFAVPFVGSLRLLLTSVLVFVLALVLLGYAFSTMARSQMQAMQL
                     TFFFFLPSLLLSGFMFPFSGMPAWAQYLGEIFPLTHFLRVVRAVMLKGASFSDVSFEF
                     SMLCLFVPIYGLLALLRFRTTLD"
     gene            740..1972
                     /locus_tag="PK812_01390"
     CDS             740..1972
                     /locus_tag="PK812_01390"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HRJ68234.1"
                     /translation="MPSPAVTPSMIEDAARRISGQLAATPCLPSRTLSDIVGAEVWLK
                     FENLQFTASFKERGALNCLMQLDGSARRNGVIAMSAGNHAQGVAYHARRLGIPATIVM
                     PVGTPMVKVENTRRHGADVKITGATLEDSARFAREHGEKHGMTFVHPYDDPAIIAGQG
                     TIAREMLAVAPAIDTLVVPIGGGGLISGIAIAAKAIKPDIEVIGVQSELYPSMYNAIN
                     DEALPMRGDTLAEGIAVKAPGKITTDLVRRHVDDILLVSEPEIEQAVSLLITIEKTVV
                     EGAGAAGLAAVVSRSRRFAGRTIGLVLCGGNIDTRLLASVLNRELAREGRLSKLSIDI
                     VDRPGQLARVAALLGEAGANIVEVSHQRVFSDLPAKGTLLEVVIETRDAHHRDETVTM
                     LRAAGFVVDLVSNPGGQR"
     gene            complement(2075..3094)
                     /locus_tag="PK812_01395"
     CDS             complement(2075..3094)
                     /locus_tag="PK812_01395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_function: GO:0033226 - 2-aminoethylphosphonate binding
                     [Evidence IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HRJ68235.1"
                     /translation="MLTPIARLLAAFLAICIAATAPAVAQTRTQLLVYTTYLTEHLDT
                     FKKAFEAANPDVEIIWHRDSTGTLTARLIAEAGAPKADAVFGLAATSLVEFSKRGMLE
                     AYAPKGLAELRPSFRDEANPPAWIGMSAWVAAVCFNRVEAGKHGLPTPKSWEDLAQPV
                     FKGRISMPDPASAGSGFITVSAWLQVLGEEKGWALMERLHKNVAVYEKSGSKPCSQAA
                     AGEFAVGISHDLAASQLAAKGAPIDIVVMQEGGGWEMDAAGIVKGTKKLDAAKKLMDW
                     AASREANQLYASFVNLLARPEVPPANPNYPEAVAKSMIRNDLAWASANRERILSEWTK
                     RFGSK"
     regulatory      complement(3138..3262)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(3301..3924)
                     /locus_tag="PK812_01400"
     CDS             complement(3301..3924)
                     /locus_tag="PK812_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68236.1"
                     /translation="MDEPALSPADERFLRYWSVNMTEAFRSSSDAWWPGFAYDAEQKQ
                     RMREIAGSEDISGGSVLTFYAIVVLGYIAFAGMASVTIMVPVISWLYPDPSKLPALPF
                     VLLLAGVCLLALGVGLPLSMRAGAWAGDRWAGGRPLPSKPGDAALVARARFQLRRIAI
                     VAAGMLVPGVLLFILFDIDAGPLVFWLKLATVLCVLLSIGALRWLRP"
     gene            complement(3917..4315)
                     /locus_tag="PK812_01405"
     CDS             complement(3917..4315)
                     /locus_tag="PK812_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68237.1"
                     /translation="MVRALWAFLASLIAGLSVVQVAATTGRADETMIVAYLLVPVVAV
                     VALIVLLIAGLGDSGAARVRRLGPLALACMALLGLLAVGWEVAKAGPATLVRYASLYL
                     ALFLGPMAAVATQWLILRRDDRTGARGSDG"
     gene            complement(4319..5428)
                     /locus_tag="PK812_01410"
     CDS             complement(4319..5428)
                     /locus_tag="PK812_01410"
                     /inference="COORDINATES: protein motif:HMM:NF018389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HRJ68238.1"
                     /translation="MVRSLGFLCGLIALTTPVAAEIGALRTFNDWLVACDNVRFCRAM
                     GVSPTGSVEGPALIVAREGQGAALPKLLLLWPDSPPGQTMILTADGETLGSLDPARDV
                     EQTEDHGGGQMIKSEAVAQKIFAALRKAKTITIAVEGSKEEPHAVSLAGASAALLFID
                     DRQKRVGTRTALVRAGDRDPSTVPAPPALPKPPSAPKVPKKAFAEEAPPAVVKLYLSE
                     AEKACDRDAKEAKEASAYLAGRLSPTQVLYAMQCWRAAYNFGAAFYVFKEGPGGGTAT
                     RAAFPAAFERATQGDVWPTHVLSNAEFDTDALEVTTFHKGRGIGDCGTAYSWRWTGTK
                     FVPTAAKSMPICRGFIEENWLQLYRVAGQNKPLAE"
     gene            5660..7030
                     /locus_tag="PK812_01415"
     CDS             5660..7030
                     /locus_tag="PK812_01415"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012254356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HRJ68239.1"
                     /translation="MLFNSFAFLLVFLPLSLAAHWAAERYAPAWRLAVLALLSFAFYA
                     YWDWRLLPLMLISIGVNWLIARMYGATQRGMLVTLAIVVNLLVLCVFKYAGFFVDTAN
                     TLGGFSWARPEIILPLGISFFTFHHIMYLVDLRAGKAPQFNLTQYALYIAFFPQVLAG
                     PLVRWSEIMHQFDQRPFREGYFDRAAQGLMLLVFGLMKKVFLGDPLSEYANPIFADAA
                     AGKTIAFAQAVQAVLAFTFQIYFDFSGYTDMAIGIGLMFGVLLPLNFNSPYVATSLQD
                     FWRRWHMTLSRFLRDYLYIAMGGNRLGLAIQLFALFATMALGGLWHGAGWTFVAWGVA
                     HGVGLGIGVLWRKNGWPMPTFLGWLATFIFVMLCWVLFRAQTFDAAMAIYQGLLGYNG
                     LGSGFKWRMLAVAAVIAIVGPTSFALSEKLRPAPWLAVLLAVVFVAVLLKIGDDANYE
                     FIYFQF"
     gene            7033..8004
                     /locus_tag="PK812_01420"
     CDS             7033..8004
                     /locus_tag="PK812_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68240.1"
                     /translation="MAGKRFLLVFCASVAAGVGVLALLLLILDPFDRGHGLFAKRGTL
                     ERGPRTANVSRGRDPAFNAAVFGNSHIQLLKPERLNAATGLSWVQLTVPATMPDEQVI
                     VMHWFLHNHPGSSKALVIGTDHFWCNADPNLSSKHPFPRWLYSLEPVSYYSNLFRMQA
                     VRLMPRKVSFLLGQTKARPTDGYWDYEAYRTRNEAQLLKEAEAARSAPLWNETGKYPW
                     IERLARELAALDRRIAVILVAPPHFQPGMPPQGSAQAQAERACAAQLATVARNRPGTA
                     FLDMRADRDAFSNPAHFWDTTHYSADVARAIETEIAKALRSIEASGK"
     gene            8626..10194
                     /locus_tag="PK812_01425"
     CDS             8626..10194
                     /locus_tag="PK812_01425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRJ68241.1"
                     /translation="MTFLQSERDGILDDVSVVRRIARVGFRIPVFGPALRWALLSALR
                     TVREVTSFSRTADFVVRGSLTLLKTTRRLPGGRTLFDRVWLPLARGSVVLAEAPERPL
                     SDFSPLLERDAFSGGPIVLCNNALAWGGVERQIVNTLNGLAAETTESLGLLCVRLDAG
                     PDYGFYLPSLDGFRGFVRNAVTPGEAAARINAMVRADRAGDLSAQIAWLPSHVQEEVR
                     RFVGEFLTLKPSVVHVWQDALSISAGMAAKLVGVPKIIVASRNMAARRFAYHQPYMAT
                     GYRELAACPDVVFLNNSEAGARDYAGWLGILPDRFELLRNGVDEASLSRPPSEATAAL
                     KARLGIPPDAPVLGSVFRFYDEKQPMLWVQAAGIVAKALPQTHFVVFGTGPLMGEVWK
                     FAERQGFADRLHLPGTIEDVALGVSLFDAFMLTSQLEGTPNVVLEASLLGVPVVATNA
                     GGTVEAIADGLTGYVARHGQAEALAELVLKVLRDPIWPARAREHGPSFISQRFGLKRM
                     IDETVALYGLKRLK"
     gene            10261..10977
                     /locus_tag="PK812_01430"
     CDS             10261..10977
                     /locus_tag="PK812_01430"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRJ68242.1"
                     /translation="MHLRPEGARMLEIGAGTGRQAQELASKGFAVEAIEILDSNYAGD
                     RLFPITNYDGRNIPFEDASFDIVFSSNVLEHVPDLVQMHSEIRRVLRPGGYAVHVLPT
                     HAWRFWTTLSAFPTAFQYAATLGGQLAPKWPFGPSEIKRLIKIWLMAARHLAAPFFQR
                     RHGERGNILSETWLFHPSWWRRNFAQNGFSIMRDEPMGLFYTGNMTFGQRWSMEKRAR
                     LAKTLGSACHIFELQPTPRK"
     gene            complement(11060..12604)
                     /locus_tag="PK812_01435"
     CDS             complement(11060..12604)
                     /locus_tag="PK812_01435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRJ68243.1"
                     /translation="MTAPRDDLGKAQGREKTGVAGGNAGWRPSLGFALRRMLDHAFSL
                     AQPVARRVLPRSVRARLAGVASLIEQATATSRMEAFRRRARPLASISPLLPADRFTGA
                     PLLVNDALAWGGVERQIVYTLQGLAARLPEPAGLLCLRLGQDENYDFYRPSLQDFGGL
                     LRNVVPASEASAILAETMTAERRRDIEQNIGWLPRDVQEDIWRFVADFVRLKPRVVHA
                     WQDSLSISAGYAARLAGVPKVIVAGRNIAPIHFAYHRPHMRLAYEELAGCDDVTMLNN
                     SVAGARSYAAWLAVPEARFVILRNGIDPNALVAASQGDVARLRAELAIPEQSPVIGSI
                     FRFYAEKQPLLWIEAAGIVASRLKHAHFVVFGTGPQREDMLRRARELGFADRVHLPGT
                     IAAPTLGLGLFDVFLLCSGMEGTPNVVLEASLMGVPVVATDAGGTAETIAEGESGFVV
                     RSANPQEIAGKVLDVLEDAGFKARAGHHGPKFILDTFGLERMIDETIALYADVGARHA
                     APGNAP"
     gene            complement(12742..13881)
                     /locus_tag="PK812_01440"
     CDS             complement(12742..13881)
                     /locus_tag="PK812_01440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRJ68244.1"
                     /translation="MKVLIVVGRLGLGGAERQALALARGLGDLGHQARVAVFRDGGAM
                     QADAVATGVAMEVLSGSNAAARIASLRGLIARERPDVVHGYLTAGNLACLCARTLTRR
                     PLLVWGIRASDMRMENYGIKWRWAAWLERRLMPLADLLIVNSQSGLEVLRAGGVPDRR
                     IVVIENGIDLARFAPHQHGIDHRRNTARVAWNCRDDTAVIGHVARIDPMKDHSGFLQA
                     LALLRHQRTGWKAVLVAVGSQEQRDRLTAEAASLGLGEDVVVIPAAADIASLYPGFDV
                     ACSSSAYGEGFSNVIAEALASGLPVVATDVGDARRIVGPCGRIVPPGAPEILADALSD
                     VLDRRDVLSGMAREQIAPYDQKRLARRTVEALEQALRRWDRQVGA"
     gene            13996..15123
                     /locus_tag="PK812_01445"
     CDS             13996..15123
                     /locus_tag="PK812_01445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRJ68245.1"
                     /translation="MRILHVISNLMSGGAESMLTKVVEALPAPRFEHAVVTFVNGGLF
                     ADRLGEAGVPIFGLGQQKGQAARALLRLSDVRRIVEEFQPDILQGWMYHGNLAASVAA
                     GRRPVIWNVRQRLERLSDNAVTTRLAILGSLGWRARVRAVIYNSEHAALEHERWLYPR
                     ARRIIIPNGFDLEAFRPDQTARATLRGELGLASDAVVVGRIARDDAIKDTPNLVRAFA
                     ALPFRDVHLVLAGRGMDSRNALLLAELRSLGIGDRVHLLGERRDIARLNAGFDLAVLS
                     SSHGEGFPNVVCEAMAAGTPIVSTDAGECRAIIGDPSRIAPIRDPQALGAVMTRVLAL
                     TSGERMALGRADRERVSRLYSLPAVAESYAALWHRIAGKQA"
     gene            complement(15152..15892)
                     /locus_tag="PK812_01450"
     CDS             complement(15152..15892)
                     /locus_tag="PK812_01450"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRJ68246.1"
                     /translation="MQSDLTPEALNRYLAVWKAKPVLRAVYGDFYDRIAAACGPGPTL
                     EIGAGIGNLPEKLPDVIGSDIQFGPWLDLVADAQKLPFKADCLGNIVMVDVLHHIEHP
                     LNFFREAARTLRPGGRVVMVEPGITPLSWAFFNYIHEEPVIMSADPLTAGAPDPARNP
                     YESNQAIPSLIATRHRERFEHLVPEMTVREVVWFSPLSYPLSGGFKKWTLLTGGMARA
                     LMALERRVERWIGRYVGFRVFLVFEKRA"
     gene            complement(15919..17370)
                     /locus_tag="PK812_01455"
     CDS             complement(15919..17370)
                     /locus_tag="PK812_01455"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRJ68247.1"
                     /translation="MTTLLAAAPLSPRKEKIRAASDAAAPDRRAWRERNAFFHEEDLR
                     YLQFLIPPGARVLEIGCGTGDLLAALKPSRGVGVDISPRMIDVARKAHPGLEFVVGDA
                     EDPATLDAIEGPFDAIVVVDSIGQMDDCQAFLARLHRLCNRDTRVIIGYFSHLWWPVL
                     KLAETAGLRTKVPEVNALSVADVKGLAELSDFELVKSEIRLLSPARLLGLGRFLNRFI
                     APFPGIRNLALRHYSVCRSQRCADPGYRSATVVVPARNEKGNIEAAVLRTPRFTDDLE
                     IIFVEGHSKDGTWEEIERVQKAYPQYDIKIMRQPGKGKADAVFHAFDRARGDVLMILD
                     ADLTMPPEQLPKYWHAISSGKAEFVNGSRLVYPMEDEAMRFLNLIANKSFSYLFSWLL
                     NQRFTDTLCGTKVISRADYQRLKAGRAYFGDFDPFGDFDLIFGASKLNLKVLEVPIRY
                     ANRSYGETQISRFRHGVMLLRMVLFAFMRIKAL"
     gene            complement(17373..18401)
                     /locus_tag="PK812_01460"
     CDS             complement(17373..18401)
                     /locus_tag="PK812_01460"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HRJ68248.1"
                     /translation="MATAKDAAVSWRKRGLVAFKLAVAVGCMVMVSRQIDPANLRKLL
                     ENLEPGWVALAILMIALEVPLVGERWRLVVTSLGRGMYQLPHGDVHAVNGFGQFVSQV
                     LPNLAGDGVRALMLRSHGVTLPHAAWSVLLDRALGVHSLFLMAFAFLLLPSGLESLGG
                     YRQPVLLTVALVAMGGTLALLSARPLGAMLERRQMLRFLGVALTETHAALLGPNAVKL
                     FAISALVHGLTILCIFALGRAIGLALPLADAAVLMVYIMVITLLPISVGGWGVRELAV
                     TALLTAHGASAEEAVVFSVAFGMTVMLATVPGALYWLFRRNASMRAAESDSAQRAPQT
                     DQTEKTGM"
     gene            complement(18657..19610)
                     /locus_tag="PK812_01465"
     CDS             complement(18657..19610)
                     /locus_tag="PK812_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HRJ68249.1"
                     /translation="MASANYALAGKRVFVAGHTGMVGGALVRRLANEGCTILVAPRSS
                     ADLRRQAEVESWMAENRPQAVFLAAARVGGIHANNTYRGDFIYDNLAIAMNIIHAAHL
                     NGVEKLMFLGSSCIYPKLAPQPMNEDQLLQGPLEPTNEPYAIAKIAGIKLAESYRRQF
                     GDDFVSVMPTNLYGPGDNYHPENSHVAAALIRRFHEAKQSGARSVVVWGSGTPRREFL
                     FVDDLADACVHLMKSWSDERLLNIGTGTDVTIRELAESIARTVGYEGEIVFDTSKPDG
                     TPRKLLDVSRLQSLGWRASTSLTEGLQRAYKAFLDGQSRMV"
     gene            complement(19597..20676)
                     /gene="gmd"
                     /locus_tag="PK812_01470"
     CDS             complement(19597..20676)
                     /gene="gmd"
                     /locus_tag="PK812_01470"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRJ68250.1"
                     /translation="MTLAEGARRVALISGVTGQDGAYLSELLLSKGYEVHGIKRRSSS
                     FNTQRIDHLYQDPHVESARFHLHYGDMTDSTNLIRLVQEVQPTEIYNLAAQSHVAVSF
                     ESPEYTANADGIGVLRLLEAIRILGMEKQTRFYQASTSELYGLVQEVPQRERTPFYPR
                     SPYAAAKLYGYWITVNYREAYGMYASNGILFNHESPIRGETFVTRKITRSVARIETGL
                     DDCLFLGNLDAKRDWGHAKDYVRGMWMILQSDEPDDFVLATGETRTVREFVDIAFSEV
                     GRKIEWRGSGVDESGVDTKTGKTLVRVDPRYFRPTEVDLLVGDASKAQQRLGWQPEIS
                     FATMVRDMVATDLTAAKKERVDGVR"
     gene            complement(20826..24014)
                     /locus_tag="PK812_01475"
     CDS             complement(20826..24014)
                     /locus_tag="PK812_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68251.1"
                     /translation="MTPNATIIRFATLGSRVLTFFAAGILAAWLVLSSGFVGVRSTLD
                     MTRYQAEIGKAFIGSLGLARTPYFYVDERYGLVGFSVLKENDLRLGAPRSLHQDIRTI
                     GNGRYSVWARYLYFSTSDNTDPRTNGRAYTLTGPVAPVSQHWLLVTGLLAAGLGIGHV
                     LRRGASRLSASAGVADRTPALLAVLPFFAGVAIALAPVVTRLRLQEATLSDLPFFLVL
                     LGIASILTPALAFPVSSLRSSYVASRIIRTVGIAAALLLALVTSGTLMGWHSVKPESV
                     QHVKGQLYTYDVSNLPLFSQFVTDEERTLSLRPILQDRNGPLTSGQSDARIEQMPGGL
                     YSALSQTFRFSYRNAGGPTAPENGMTVNGSLRFDRAITLLLLGMLLSAVLVSNLALRS
                     GFAVAVDKAPDLRRAMIAGSILFVLGLAGMLIPSSLFPATETALTLKLRLTQAITLTG
                     AVLVLSCLPWRVLVQDVRSRPGPAASVAILLAAFAMPFAGLADAWIASGRINTHSQTG
                     FHILGSMQASDSFGYLHGALELLTTGTLNDWNSRRPLTGVLTTLRMLAGAPDMQVIMG
                     LGALLVAAGLTLATREIMLALGGWAALAFATACGAFVSEWTPTTLSESTGFAMGAFGL
                     AAILAAVRRDQAALFGLGVMVLAIGLMARAGPFFLLPLVILAGAANQWRRGRKQMALW
                     LVAGGVGGVLGFAHSPYLVKMLGGSSGLIQSNFSYTLYGLAVGGKGWQQIFVDHPQLF
                     ASTMKTPPEPAIYAFAIEAIRAKPSLIVKALMDELLRTPEFFLTYFDLTVINIWLIFG
                     ALIALVSFRNPVALIGLAAAAGIVLSSPLLLTDGGTRVYAAGVPLMGLLVALGFDAFR
                     RGLANLMQGAGAFDLWRTLTSPTLPPAMEPAVTRRLSLAGVGAFAVVALIAAAPLIIG
                     PGKLKPVDQIAALDACPAGERRLYAEGRIFADIFDVRADSEPGFLLSPTVRQSELLHA
                     TKIIGPVFAQALAKASVPYTLRAVAEVQKPENPRAVYPLLAAFPKAVSIPRESRYWSA
                     CVATIGHADSDSIGGLEVVEIRSVQPAQLITR"
     gene            24314..24934
                     /locus_tag="PK812_01480"
     CDS             24314..24934
                     /locus_tag="PK812_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68252.1"
                     /translation="MNEVPKSLGPALILVCSYRGAADRTAPSDLVGWKRTLVDAVTLR
                     KLREYVATWRDFYANPRNAAYQRRLAEAVAARCAPGSSIVVDARLAPEAVPVTGVEAD
                     RVSRRDLGAEKAADLPSAPVVVFVYPDPLGLSWQRLEAAIAAQGRSMFIVNGRGRLFR
                     LDRQAARALRLRRFMATTRIAELLAGLMLVPISGIFALRDRLNGRT"
     gene            24937..25860
                     /locus_tag="PK812_01485"
     CDS             24937..25860
                     /locus_tag="PK812_01485"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009465845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRJ68253.1"
                     /translation="MLDTEKADPASSRATVMKEKIRRWWAEAPMTYGETHGTTEYHLP
                     DGSVETVEIGTRRFFELADQRFYSWNTPLHQPDGPFSAIFDYKRFEGKRVLEIGCGMG
                     CMAMNWAQRGALVTAVDLNPTSVSQTKLRFKQFGLDGDIRESDGESLPFEDESFDYVY
                     SWGVLHHTPGTREALAEVYRVLKPGGQFGLMLYHRNSFLYRYTIAWVEGFVNMESRFL
                     SPLELASRYSDGAREDGNPHTWPVTKREVRRDLVSQFGGVDIKVLGTDVPVILNVWLP
                     RLAEKLLPLSWQKAMARRFGWSLWITGGKSL"
     gene            25860..26855
                     /locus_tag="PK812_01490"
     CDS             25860..26855
                     /locus_tag="PK812_01490"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRJ68254.1"
                     /translation="MTASSLTRFARRVRYAVAHPTAVVRYLVGTPAAPAMGATAVAED
                     APPVELAQPDPLQPFERYLALGRAQGIEQPTLFLSFDCDTDLDFTAALEVKRFLDSLG
                     IKATWAVPGVQIERGADTYRSIAATGAEFMNHGQLPHTAWGEDRYISITWYHEMTPED
                     VTADILAGDATIRRVLGVTPGGFRAPHFGHYAAPDQLALVHACARQLGYSYCSTTLPA
                     YGLGNGPLHDADGIVELPTFGSLVAPTSVLDSWSYLTDRRVYQLGDVYAELMCATAER
                     LVSERAAALLTWYADPSHVVGQAPFERAMQRLVSLGIPSLHGSEAAALLRRASPG"
     gene            26871..28736
                     /gene="asnB"
                     /locus_tag="PK812_01495"
     CDS             26871..28736
                     /gene="asnB"
                     /locus_tag="PK812_01495"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HRJ68255.1"
                     /translation="MCGIAGWLDQERRGDAADLARVRRMCDTMVHRGPDDDGYITLPG
                     VALGMRRLAIVDISGGHQPMASDDGAIQIVFNGEIFNHDALRSELAAGGTHFRSRSDT
                     EVILRLYEQFGLAAIDRLNGMFAIAIWDGRDKAMHLVRDRMGEKPLYYLWDGRRLLFG
                     SEIKAILAGLDQRPPVNPQALWDFFTFRYVPPDHTIWKSISKLPPAHRLTIRAGGGVP
                     EIARWWDIPRPSHRQRSDSDEVAQFDALLTNATQLRMLADVPVGILLSGGLDSSVVAA
                     KAALEGQRVKTFSVAFEGAEAIDERRYARMVAKAFNTDHHEVVIGEREFVDFLPDFVR
                     FTDEPLADLASIPLHYVCKLARKNVTVVLSGEGSDEILGGYSFDNWAARWDEAATLSK
                     PLTLMERLLRRQAPWVDPGLLDLRDAPQPLTMTSYMTSEEKRALFRAGMEAPDSLDGL
                     RAKLAHFGKASPLAQALYIYCQDWLVEDLLMKADKMSMATSVELRAPFLDHRVVEAAA
                     RLPDHLRVGRRADGTYETKRILRMLAAKCAVPQEILTRPKMGFPVPVYGWLSDSLRPF
                     AHDLLASPGTRLNDWCDPAALAAILARGTAPDAPMQDRHVLWNTLILEIWMRSWL"
     gene            28727..30004
                     /locus_tag="PK812_01500"
     CDS             28727..30004
                     /locus_tag="PK812_01500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRJ68256.1"
                     /translation="MAVTKRIVAVTWTLPPIVLPRSIQVSRSMAELTRRGWDVHVITA
                     PLDANNPAIPHDEALADIYRGAYAQTEIEFREGRQRSSTWRRFLRYHQEQRSFDDDNW
                     IIRAASAARRLLRRKDSVFVSFAQPWIDHLVGLRLKRIRPSTRWVAHFSDPWVDSPYY
                     DANNPAQTARMTLWRQQERAVIKHADAVVFVTAETADLVMRKYPAAWRSKVHVTPHGF
                     DRRLMPNQPLGGEDPKAGRPLRVIYTGNIYEGRREPVPLFTVLRDMDRDGELRGRVHF
                     DFYGNSPVATARQASDMGLDGIVSFHSSVGYLESLQLIAKADLLLLLDANADVNVFLP
                     SKIVDYMMTGKPILGLTPPVGASASVLRKTGNLYLDPNDKATMRTTLKALIEAHHRSE
                     PLCQLRMDAVEAFDIRRTTDSFEAALSGVIRKG"
     gene            30010..31119
                     /gene="wecB"
                     /locus_tag="PK812_01505"
     CDS             30010..31119
                     /gene="wecB"
                     /locus_tag="PK812_01505"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009465857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HRJ68257.1"
                     /translation="MKKVHIVAGARPNFMKIAPLYHALAGHPWLKPVVIHTGQHFSAN
                     MSDDFFVDLKLPAPDFHLGARGETHARQTAAIMTAYEDLALAHRPDWVVVVGDVNSTV
                     AAALVAKKLELPVAHLEAGLRNFDRTLPEEINRIVTDSISDLFWTPSPDADDNLRREG
                     VPAERIDRVGNIMIDSFLMLESAIAAVDMPRRIGLSGRPYVVSTLHRPANVDHEAPLR
                     EIVDAYVALSAEVDVVFPVHPRTDARLKAFDLRRKLERPGIKLLEPLRYMEFMSLVLR
                     ASLVVTDSGGVQEETSFLGIPCLTLRPNTERPITVTEGTNRLIGTESLLSASRTALSG
                     PRPERPIIDRWDGQAARRVVASLERHASSTPAGKS"
     gene            31121..32863
                     /locus_tag="PK812_01510"
     CDS             31121..32863
                     /locus_tag="PK812_01510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRJ68258.1"
                     /translation="MDQLASLAEDFRSEIRKVGSIWIVAPAVEHLAELMAFEGVAGAI
                     LQVRDDALAAAYPGRIGWIDWTLHEICMPDASAQCVISLFSRPEISLTVLKLMRQRGI
                     SKIGYFSIKTGRFHADLLPIAAFKRLVNAIYFRIFEEPLRVVRSPVIKNTESAEEKDW
                     SIALGRITSQTTPLLPEKAFKASKIIIVSGSLAAGGSERQVTYTALGLRKRDLSPQIV
                     CSDLEGSNAFFVPRLTEQGISAIQLPPMPGYFSRSRALLELNADIRSMVDGEDIFTDE
                     IARYAALFLSERPGVVHAWLDWTNAAAGLAALAVGVPRIVLSGRSLSPTHFAFLRSFM
                     RPAYQELLKSDRVVLLNNSRAGADDYASWIGVDPSRIKVIPNAIDPAEHPVPDPASVR
                     AFRERYNLAEGTPVLGGVFRLMPEKRPDLWLKIAAEVAEVRPDARFMIVGGGPERASI
                     EAYARQLGLGEKLILTGEASDVVTPLAVMSCFLLTSRVEGLPNVLLEAQLSGVPVVTS
                     AVGGAPETIDSDDDTSLAIDGDDPAPYVRAVLAILGNPERLRQSKTTLPAIIGRKFGF
                     DAMIDRTLGIYFDK"
     gene            33012..34082
                     /locus_tag="PK812_01515"
     CDS             33012..34082
                     /locus_tag="PK812_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRJ68259.1"
                     /translation="MLQSEAAAVSSASAHKAEARPAVSVVCPFFNEEAIIERAAQRML
                     DNLTTQFGDEFELILVNDGSRDHSLERITALLATHPRSAQVRVLTYAFNQGRGRALKT
                     GIDAARAEIIVTTEVDCSWGDDIAQRLHAELVANPQADFVIASPHREGGSLVNVSASR
                     AFLTKAGNWLIRLFFDSRVTMNTGMTRGYRARVIQPLMTEENGKEFHLEVLLKLLGLG
                     FRVREIPATLTWLDEKLQRANSAKRKSSTRILKTINSHLRFIAIAQPVRHYAALTALT
                     VLAGVAFLMAAVAMLITKDAPAVYLAIVGLNLLVIALLFGGFSILFFQLRELSRLNWM
                     KGYESRTPPTALPVHTAFPAQR"
     gene            34092..36017
                     /gene="asnB"
                     /locus_tag="PK812_01520"
     CDS             34092..36017
                     /gene="asnB"
                     /locus_tag="PK812_01520"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HRJ68260.1"
                     /translation="MCGIVGIAGRHDPDLVRRMADAIVHRGPDGAGFHNCASAGICVG
                     MRRLAIIDLVTGDQPLHSADRKVHIVYNGEIYNHREVRLELEKLGHGFKTSSDTEVVL
                     ESYLAWGGEAWQRLHGMFALAIIDERGHRPKLILVRDRPGIKPLYLARQGNTLLFASE
                     VKALQADAGLGSEVDLNAVRAYLSLRYTPGPGTLLSGVEKLPPGHELTWCDGDVRVRR
                     WWTPPAPTVVEPGLSLDGAADRLGAALRTSVHRHMIADVPVGAFLSGGIDSNIIVALM
                     AEAASKPVKTFSIGFPDFPDDDRNLAALTARRLGTDHHPIDCRAEDMAMLPDIAFKLD
                     EPVGDAIVVPMSVLAREARQHVKVVLSGEGADEILGGYMFHRKLKQIAKVKSVVPSAV
                     FGLAADVASVMPVAVLERLFDYPGALGEEGKRKIVEMTRRIGSASLSSLYRSAVSLFD
                     PDDIRALAGHPALARLADVAPGPTLAETPGATALQALVDMQYPDWLPDDILMKADKMS
                     MAHSLEGRVPFMDEIVISAAASLPDHTKIGRAGNKLALRRFASRLLPEEVVRAPKRAF
                     YIPLESYARTQPLAGIFDWALDSARVKRRGLFSPEWIAAQRNAGPEKGFLPLKRLFSI
                     VMLELWFERFAPNVGWT"
     gene            36045..38228
                     /locus_tag="PK812_01525"
     CDS             36045..38228
                     /locus_tag="PK812_01525"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-domain-containing oxidoreductase"
                     /protein_id="HRJ68261.1"
                     /translation="MKIVVQNFKTGVLSVSDSPAPRAGAGQVLVRAHASLISAGTDRA
                     IIGLGKKSYLGKALDRPDLARKVINRARTEGLWPTYKVVKNLIAEPIPLGYSLVGTAI
                     EVGRDVGDISVGDRVACAGLGYANHAEVVAVPKNLCVRVPDGVSDEQASYVTLGAIAM
                     HGLRQADQQLGASVLVVGLGLVGQLTVQLCVAAGLRVAGLDLDPGKLTLARQAGASLA
                     MTPSDPSLGAAIAELTGGYGIDAVLLTVGSRDSGAVFEDVARLCRDRGRVVVVGDVKM
                     DISRRTYFEKELEILQSRSYGPGRYDTAYEAAGHDYPIGYVRWTERRNLRSFLDLVAD
                     KRIDPATLTTHRFSIDEAAQGYDLVTGKTKAFSVGILIQYPPREIEQAVSARPAAGAV
                     SGKIGLGVIGAGTFAKGILLPALMDTNAFRMHGVASGRGISAKSVADRYGAGIATNDA
                     RQLLDDDSIQAVVIATRHDSHARYVIEALKRGKHVFVEKPLAITAEELDAVEAAARST
                     SATLTVGFNRRFAPLVQQMAAHFAGRVEPMAMLYRINAGRIPLKSELAWVHDRATGGG
                     RIVGEACHFVDTMQFLCGALPVSVQVTSANPRRADVAHDDIVTFTVSFDDGSIGSVHY
                     WSNGDASYPKERFEAFAQERMALLDNFRALDLVAGNKTTRKRSFNVDKGFAAEAAAFA
                     RACRGGDPGIALDSLLATTRVTLAVAGALGGAGAHVDDPMAEAHD"
     gene            38221..39108
                     /locus_tag="PK812_01530"
     CDS             38221..39108
                     /locus_tag="PK812_01530"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRJ68262.1"
                     /translation="MTEAGSPCVVCGSVAQRRARVRLRYGTIFECAGCGTGLLEPRPT
                     AAGLSALHSEEAYFSHPYFEDRRELTAAMRASSEAKLSRIEARVGSLAGKTLVDVGCD
                     LGVLGAYATESRGMTAIGIDIIAKVVEIGRAAGRDLRHGTLESVALPSGSADLICGFD
                     LIEHVDDPRGLAREAWRVLKPGGVLALETPNYNGLVYRIGRWLGGAPGLASVIAGLQE
                     RLWPPFHVQYFTADSLRNVLDGAGFADVKVQGRELDTSELAVSSPVLRAAVLSVFAAA
                     RVTGSATLLTAIARKPRQR"
     gene            39114..39464
                     /locus_tag="PK812_01535"
     CDS             39114..39464
                     /locus_tag="PK812_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68263.1"
                     /translation="MIRLLASGVVGGCIVGGQMCLAAFARALAPPFTIANLASQAFAM
                     PWLYLAGAAYSVAVLLYLILLRTGAISATNLPIMAVIVVLNIAIALSPGDALTARQIT
                     GAVLVGAGTLLMLN"
     gene            39474..41102
                     /locus_tag="PK812_01540"
     CDS             39474..41102
                     /locus_tag="PK812_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68264.1"
                     /translation="MAHADPYVHAITPLLGRALSGLDAEITSPSRGSFDRTWWCWKFT
                     DFSATRFQEGAHVLAWLATSPVSPAPEASRPRFAQLCGASLAFWARLQHGDGSFDEAY
                     PFERSLAATAFSAFYLGRAIERLRGALAPEVESLALRTLERSAGWLAANGEYHGILSN
                     HLAAAAGALQIAGDVLQTDRFRSGRDRYLGLIYRHQHPSEGWMREYGGADPGYQSHGM
                     FYLAEIERRTRDGELFERLSRGCDFLRWFVHPDGTIGGEYASRGTKFSYPAAFELLAA
                     RLPAAASIAAHLREQLRDGRGVGAREMDAWNQFPLLNNLLFAADAAGDLGRPAPLPWH
                     QDGSTATFPDAGLVIARRGRRIIACGANMGGAVKIWSADGALEYEDCGYAAQSADRWF
                     VSQGEGSAHIDAVAETALTVTAKTPFRGIPTTRFDPWRFIAFRLFTTTIGRLPSVARW
                     LKELLVKVLIRKKQPHPGSLLRHVTIASDGSVTIDDTISDVATAPVPLPRHVPFHMGS
                     SRYADFADWCGAERPCPAPTASEGGYRRRLVLDA"
     gene            complement(41111..42037)
                     /locus_tag="PK812_01545"
     CDS             complement(41111..42037)
                     /locus_tag="PK812_01545"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRJ68265.1"
                     /translation="MPDQVESCPVCGSGRVHLHRSDVVDFEYGVRPDREFRIARCDDC
                     QSDFLTPRPTVAEIASFYPDDYHAYHDDHSGVARLLVSLRSRARASSYRGMIGSEGGR
                     LFDVGAGDCRHFDALKPYCSLEFSGVEIKPEIAEKARARGYDVETGTLEEMDLARHLG
                     RYDIVSMNHVIEHVLDPREMARRAFAILKPGGRALGQLPARDCWEESLAGRCWGGYHF
                     PRHLQAFSYGGLARCLTEAGFVDVKVRSAPHVQAALSVQNTLISKGWKPSMRFGKTPV
                     YSWIILAVLPFETVAWLAGRGGVMDFSARKPG"
     gene            complement(42030..43028)
                     /locus_tag="PK812_01550"
     CDS             complement(42030..43028)
                     /locus_tag="PK812_01550"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HRJ68266.1"
                     /translation="MTKQAAESRALNDPRRYITDAELRAFHKPRPWIVARDLAFDWFL
                     ILGALALWGWTRHPLVFVLSFVVISARQHALNNWVHEASHFSLTRNKALNDWICDIFA
                     ATPHFIATRDYRSKHKLHHTDLGDPEKDTEIKSRFIIKGLRFWKRCILTLLGSSALLT
                     AKTYASQSLGDPEDGRQRLRFLVLTAGTNGVILAWCWYWGAPFAWLYLWFLPLLTLTS
                     LLATIRVIAEHQSDEYALAGIEQFDQQLDPAITRTISTDWLTAFLLAPVKFNYHFEHH
                     VWPAVPYTSLPALSQLLHDRGFYKANPHAYGRSYYQVLGRLIHPEAVSSSKDTVHA"
     gene            complement(43093..>45147)
                     /locus_tag="PK812_01555"
     CDS             complement(43093..>45147)
                     /locus_tag="PK812_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68267.1"
                     /translation="GPGVGVFIGLLLTHWFALGSRLLPQPGAIAAMALGTAALFLPWH
                     SVRRQSDRIVEHLQSKARDALRATAPSPVWAIILVVGAWTVLLWRLAGLNPNYSDTIA
                     PALWLTGELVAHPGTIQAEVYPHLQVLLPRLAAMLQKAFGLPVQALHDGLAAVFAMLG
                     LGAVAMTAAKLSRTSGWVGLLAGCLAIAALVQAWPGKMHLGYGLLMHMPGYLLGAWAL
                     AAALAIWSWWLFSARSMRANVIAFGLAGLLFDLHSTFGVILLGTMLTGLGLEALSQRA
                     FAVAIRSCVAGLAAFLVCASPQMVMLMLHALASPVAQGPIPDPAAWWTLMAARKPSHI
                     FIWGENGVVQPLAWLAALWSLAVFAIWPLVASERRLRLVATGLAALVFSAMAYVAFAW
                     RPTPTIAGLVLTRATLICVATLPALLAALLVLNVRAWSQSGNLASLGSAALAGLSLAL
                     ISLPEAGPELRSLALVLLALAGLAARTLGPPRTPGAAVSLGRVVAMVACAVSLAVPLW
                     LVPAKARETLASMPRPDGPEWSEVTRFLRERTEPAEMALLPPYPYAMMSSRKASPTDY
                     GQLAFSVYARWLLPFEFNQLMVLYGIDLRDRPAHSVAAWLAEQGGLLCMIERGYVHLV
                     SDAGRLQALRRHYPALRLIVAPKDGTEPEGWTCGPVKLPQLPLERAFENSRFVVYKLA
                     TG"
BASE COUNT         8246 a        14599 c        14289 g         8013 t
ORIGIN      
        1 gtcagcgcgc ttgccgccat cgcggcccgc gccctcttgc gagaacaagg gcttgaggtc
       61 gcggccgacg agcaaaaggc gcgccagatc gatgttgtcg tgcagcggcg ctacaacccg
      121 gagggcatct cgcaatacaa catcgtgccg ggcctgctcg gtgtcatcct gcagatgacc
      181 atggtgatga tgacggcaat ggcgctcacc cgcgagatcg agcgcgggac catggaaaac
      241 ttgctggcca tgccggcaac cgcgacagag atcatgctcg ggaaggttct gcccttcctc
      301 attgtcggcg gggtgcagat ggcggtggtg ctggtcgcgg ccaaggtgct gttcgccgtg
      361 ccgtttgtgg gcagcctcag gttgctcctc acctccgttc tggtcttcgt gctggctctg
      421 gtgctgcttg gctacgcctt ctcgaccatg gcgcgctcgc agatgcaggc aatgcagctg
      481 accttcttct tcttcctgcc ctcgctcctg ctttccgggt tcatgttccc cttctcggga
      541 atgccggcct gggcgcaata ccttggggaa atcttcccgc tgacgcattt cctgcgcgtg
      601 gtgcgcgccg tcatgctgaa gggcgccagt ttttccgacg tcagcttcga attctcgatg
      661 ctttgcctgt tcgtcccgat atacgggctg ctcgcgttgc tgcgtttccg cacaacgctc
      721 gattgaccca ggagtgccca tgcccagccc cgccgtcaca ccttcgatga tagaggacgc
      781 cgcaaggcgc atcagcggcc agcttgccgc gacgccctgc ctgccatcgc ggacactgtc
      841 ggacatcgtc ggggccgagg tctggctgaa gttcgagaac ctgcaattca ccgcctcgtt
      901 caaggaacgc ggcgcgttga actgcctgat gcaactcgat ggttcagccc gccggaacgg
      961 cgtcatcgcc atgtcggccg gcaatcatgc gcagggcgtc gcctaccacg ccaggcggct
     1021 cggaattccg gcaaccatcg tcatgcccgt cggcacgccg atggtgaagg tggagaacac
     1081 ccggcgccac ggcgcggacg tcaagatcac aggggcaacc ctggaggact ccgcacgctt
     1141 cgcccgcgag cacggcgaga agcacggcat gacgttcgtg cacccctatg acgatccggc
     1201 gatcatcgcc ggccagggca cgatcgcccg cgaaatgctg gcggtggcgc ccgcgatcga
     1261 cactctggtc gtgccgatcg gcgggggcgg attgatctcc ggcatcgcca tcgccgccaa
     1321 ggcgatcaag ccggacatcg aggtgatcgg cgtccagtcc gagctttatc cctccatgta
     1381 caacgccatc aacgacgagg cgctaccgat gcggggcgac accctggccg agggcatcgc
     1441 cgtgaaggcg ccgggaaaga tcaccaccga tctcgttcgc cgccacgtgg acgatatcct
     1501 gctggtgagc gagccggaga tcgaacaggc tgtcagcctg ctgatcacca tcgagaagac
     1561 tgtcgtcgag ggcgcgggcg cggccggcct ggccgccgtc gtcagccgct ccagacgctt
     1621 cgcggggcgc accattggcc tcgtgctgtg cggcggcaac atcgatacgc gcctgctcgc
     1681 cagcgtgctc aaccgcgaac tggcgcgcga ggggaggctc tccaagctct cgatcgatat
     1741 tgtcgaccgt ccggggcagc ttgcgcgcgt ggccgcgctt ctcggcgaag cgggtgcgaa
     1801 cattgtcgag gtgtcgcatc agcgcgtgtt ctccgacctg ccggccaagg gcacgctgct
     1861 ggaagtggtg atcgagacgc gcgacgcgca ccatcgcgac gagaccgtga cgatgctgcg
     1921 cgcggcaggg ttcgtggtgg acctcgtcag caatccgggt gggcagcgct aagccacgcg
     1981 tgtcgacggt gtcgttccgg agcagcgaag cagatcccgg attgaaggca tcgagcgcaa
     2041 gacggggctt tgccggagtt gccggttcag ccccctactt cgagccgaac cgtttcgtcc
     2101 actcgctgag aatgcgctcc cggttggctg aagcccaggc aagatcgttc cggatcatcg
     2161 acttcgccac agcctcggga tagttcgggt tcgccggcgg cacctccgga cgggccaaga
     2221 gattgacgaa ggaagcgtag agctggttgg cctcgcggct cgcggcccag tccatcagct
     2281 tcttcgctgc atcgagcttc ttcgtgccct tgacgatgcc ggcggcgtcc atctcccagc
     2341 cgccgccttc ctgcatgacg acgatgtcga tcggcgcgcc cttggcggcc agttgggagg
     2401 cggcgagatc atgcgagatt ccaacggcga actcgcctgc ggccgcctgc gaacagggct
     2461 tggagccgga tttctcatag accgcgacat tcttgtgcag ccgttccatg agcgcccagc
     2521 ccttctcctc gccgagcacc tgcaaccagg ccgagacggt gatgaacccg gagccggcgg
     2581 aggcgggatc cggcatggag atgcgtccct tgaagacggg ctgcgccagg tcttcccacg
     2641 acttcggcgt cggcaggcca tgcttgcccg cctcgacgcg gttgaagcag acggcggcga
     2701 cccaggcgct catgccgatc caggccggtg gattggcctc gtcgcggaaa ctcgggcgca
     2761 gctccgcaag ccccttcggc gcgtaggctt ccagcatgcc ccgcttcgaa aactcgacca
     2821 gcgaggtggc cgcaaggcca aaaaccgcat ccgccttcgg cgcccccgcc tcggcgatca
     2881 ggcgtgccgt cagcgtgccg gtggaatcgc gatgccagat gatctccaca tcgggattgg
     2941 ccgcctcgaa ggccttcttg aacgtgtcga gatgctcggt gagataggtc gtgtagacca
     3001 gaagctgcgt cctggtctgc gcgaccgccg gtgcggtcgc agcgatacag atggcaagaa
     3061 aggcggcaag aaggcgtgcg attggcgtca gcattatccc attccctcga ttgcgggatg
     3121 ggacacggat cacggcttcg cgtgctgctt cccatccctt cgccggcatg acccggatca
     3181 ggttcgaagg gtcgccgcgg ggcaacgcaa agcgcgccaa tcggcttctc agccccttgc
     3241 cggggctccc ctaggtgagc gtgaacgtag cgtcgggaac cggcgcgtca agtgcggcgc
     3301 tcagggcctc aaccagcgaa gcgcgccgat agacaggagg acgcaaagga ccgttgcgag
     3361 cttgagccaa aacaccaatg gcccagcatc aatatcgaac aggatgaaca gcagaacgcc
     3421 ggggacgagc attccggcgg ccacgatggc gatgcggcgc aactggaacc gcgcccgagc
     3481 gaccagggcc gcatcccccg gtttcgacgg cagaggcctg cctccggccc agcgatcgcc
     3541 cgcccatgcc ccggcgcgca tggacagcgg caacccgaca ccaagagcca gcaggcaaac
     3601 gcccgcgagc agcaggacga aaggcaaggc cggcagcttg gaaggatcgg ggtaaagcca
     3661 ggaaatgacc gggaccatga tggtgaccga cgccatgccg gcgaaggcaa tgtagcccag
     3721 caccacgatg gcatagaacg tgagcaccga accgcctgaa atgtcttccg aaccggcgat
     3781 ttcgcgcatg cgttgtttct gctccgcgtc gtaggcgaac cccggccacc aggcatcgct
     3841 gctggagcga aaggcctcgg tcatgttcac cgaccagtag cgcaggaacc gctcgtcggc
     3901 cggtgaaagc gccggttcat ccatcgctgc ctcgcgcgcc cgtcctgtcg tcgcgcctca
     3961 ggatcagcca ctgcgtcgcg acggccgcca tcgggccaag gaagagcgcc agatagaggc
     4021 tggcgtagcg caccagcgtg gcaggtccgg ctttggccac ctcccagccg accgcaagaa
     4081 ggccgaggag cgccatgcag gccagcgcca gcggcccaag gcgcctgacg cgcgccgcgc
     4141 cggaatcgcc cagccctgcg atcagcagca caatgagcgc gaccaccgcg accacgggta
     4201 cgagcaaata cgccacgatc atcgtctcat cggcgcgacc ggtggttgcc gccacctgca
     4261 cgacggacag gcccgcgatc aacgacgcaa gaaaggccca gagcgccctc accatcgcct
     4321 attccgccag cggtttgttc tggccggcga cccgatagag ttgcagccag ttctcttcga
     4381 tgaagcctcg gcaaatcggc atggacttgg ccgcggttgg aacgaacttc gtcccggtcc
     4441 accgccagga atacgccgtg ccgcaatcgc cgatgccgcg ccccttgtga aaggtcgtca
     4501 cttccagggc atcagtgtcg aactcggcat tggagaggac gtgcgtcggc catacgtcgc
     4561 cctgtgtcgc gcgctcgaag gccgcgggga aggccgcgcg cgtcgccgtg cccccaccgg
     4621 gcccttcctt gaacacatag aacgccgcgc cgaaattgta ggccgcgcgc cagcactgca
     4681 tggcatagag cacctgggtc ggggacaggc ggccggcaag gtaagcgctg gcctccttgg
     4741 cctccttcgc gtcccgatcg caggcctttt ccgcctccga cagatagagt ttcaccaccg
     4801 ccggcggcgc ctcttccgca aacgccttct tcggcacctt tggcgcggat ggcggcttcg
     4861 gcagcgccgg tggcgcagga acagtcgagg gatcgcgatc gccagcgcgg accagcgccg
     4921 tgcgcgtgcc gacgcgcttc tgccggtcat cgataaaaag caacgccgcc gaggcgccgg
     4981 caagcgagac ggcgtgcggc tcctccttgc tgccttcgac cgcgatggtg atcgtctttg
     5041 ccttgcgaag cgcggcaaaa atcttctgcg cgaccgcctc ggacttgatc atctggccgc
     5101 cgccgtggtc ctccgtctgc tcgacgtccc gggctgggtc gagactgccc agtgtctcgc
     5161 catcggccgt gagaatcatg gtctggcccg gcgggctgtc cggccacaga agcaacagct
     5221 tcggcagggc cgcgccctgc ccttcccgtg ccacgatcag cgccgggcct tcgacggaac
     5281 ccgtcggcga aacgcccatg gcgcggcaga accgcacgtt gtcacaggcc accagccagt
     5341 cgttgaacgt gcgcagcgct ccgatttcag ctgcgacggg agtggtaagc gcgatcaagc
     5401 cgcacaggaa acccagggaa cggaccattc atgactccca atggcgcacc gacatgcgcg
     5461 gaaggtcgac ggggccccaa ccccagcaga ctagcaaaga cgccagaggc tgcaatggct
     5521 ctgcggacgt tccaagggcg agacccttgc cgcagcgagg cgccaggcct gcgcgtgtcg
     5581 ggtgcgattg gacatcgatg agcgttccgc tatgagagcg ctccgagtgt caaccgccaa
     5641 gaccggcccg caaagtcccg tgcttttcaa cagttttgcc ttcctgctgg ttttcctgcc
     5701 gctgtcgctt gcagcgcact gggccgccga gcgctatgcg ccggcctggc ggctggcggt
     5761 gctcgcgctg ctgtcttttg cgttctatgc ctactgggac tggcgcctgc tgccgctgat
     5821 gctgatttcc atcggcgtga actggctcat cgcacgcatg tacggtgcga cgcagcgcgg
     5881 catgctggtg acgctggcca tcgtcgtgaa cctcttagtg ctctgcgtgt tcaagtacgc
     5941 agggttcttt gtcgacaccg caaacactct tggcgggttt tcctgggcac gaccggagat
     6001 catcctgcct ctgggcattt cgttcttcac cttccaccac atcatgtatc tggtggacct
     6061 gcgcgccgga aaggcgccgc agttcaatct gacccagtac gcgctctaca tcgccttctt
     6121 cccgcaggtc cttgccggcc cgctggtgcg ctggagcgag atcatgcacc agttcgacca
     6181 gcgcccgttc cgggagggct acttcgaccg cgcggcacag ggcctgatgc tgctggtgtt
     6241 cggcctgatg aagaaggtgt ttctgggcga tcccctttcg gagtatgcca atccaatctt
     6301 cgcggatgcc gcggctggaa agacgatcgc cttcgcgcag gccgttcagg ccgtccttgc
     6361 cttcaccttc cagatctact tcgacttttc cggctatacc gacatggcga tcggcatcgg
     6421 cctgatgttc ggcgtgctgc tgccgctcaa cttcaacagc ccctatgtcg caacctcgct
     6481 gcaggatttc tggcggcgct ggcacatgac gctgtcgcgg ttcctgcgcg actatctgta
     6541 catcgcgatg gggggcaatc gtctcggcct ggccatccag ctcttcgccc tgttcgccac
     6601 gatggcgctt ggcggcctct ggcacggtgc gggctggacc ttcgtggcct ggggcgtcgc
     6661 acacggcgtc ggcctgggca tcggcgtgct ctggcgcaag aacggatggc cgatgcccac
     6721 gttcctcggc tggctggcga ccttcatctt cgtgatgctg tgctgggtgc tgttcagggc
     6781 gcagaccttc gatgccgcca tggcgatcta tcagggcctg ctcggctaca acgggctggg
     6841 cagcggtttc aaatggcgca tgctcgcggt ggcggcggtg atcgcgatcg tcggcccgac
     6901 cagtttcgcg ctgagtgaaa aactgcggcc ggcgccctgg ctcgccgtgc ttctggccgt
     6961 ggtgttcgtg gcggtattgc tgaaaatcgg cgacgacgcg aactacgagt tcatctactt
     7021 tcagttctga ccgtggctgg caaacgtttc ctcctggtct tttgcgcctc ggtcgcggca
     7081 ggcgtcggcg ttctggcgct cctgctgctg atcctcgatc ccttcgatcg cggacacggg
     7141 cttttcgcga aacgggggac gctggaacgc ggaccgcgca cggccaacgt cagccgcggg
     7201 cgtgatcccg cgttcaatgc cgcggtgttc ggcaattcgc acatccagtt gctcaagccg
     7261 gagcgtctga atgccgcgac cggcctgtca tgggtgcaat tgaccgtccc ggcgaccatg
     7321 cctgatgagc aggtcatcgt gatgcactgg tttctgcaca atcatcctgg atcatccaaa
     7381 gcactcgtca ttggaaccga tcacttctgg tgcaacgccg atccgaatct ttcatcgaag
     7441 catccgtttc cgcgctggct ctacagcctg gaaccggtca gctattattc caacctcttc
     7501 cgcatgcagg cggttcggct gatgccgcgc aaggtgtcct ttctcctcgg ccagacgaaa
     7561 gcccgaccga cggacgggta ttgggactac gaggcctacc ggacgcgcaa cgaggcgcaa
     7621 ctcctgaaag aggcggaagc cgcgcgcagc gccccgctct ggaacgagac cggaaagtac
     7681 ccctggatcg agcggctcgc gcgcgaactg gcagcgctgg accggcgcat cgccgtcatc
     7741 cttgtcgcac cgccgcattt ccaacccgga atgccgcctc agggctccgc ccaggctcag
     7801 gccgaacgtg cctgcgcggc gcagcttgcc accgtggcga gaaatcggcc gggcaccgcc
     7861 ttccttgaca tgcgagccga cagggatgcc ttcagcaacc cggcccactt ctgggatacg
     7921 actcactatt cggccgatgt cgcgcgagca atcgagaccg agatcgcaaa ggccctgcgg
     7981 tcgatcgagg ccagtggaaa gtaatctcct gcgtcctgtc gacgcagggc ccgtagcgcc
     8041 gaagagcgcg cgcgtccctg gcctgcccgc cgccccatgg gtgcttgccg gttgcggacg
     8101 ccagaccgag acgacccgga tctttgcgct ttcatacctt gccgcctgtg gcgggcctgc
     8161 gaacaacgca ggtcgagccc tgtccatttt ccgtgtagcc gcacgaaatc agaggtttcg
     8221 agcgtcccgt gcgatggggt ctcggccctg tggtgattcg atacggtcgc gtaacagagc
     8281 gccggcaggg actggcagcg ccgctcgacc tcctgcctga gtgcacgaca ccaccgcgcc
     8341 agcagcatcg tgccaacgca ccggcctggg atgcacgctt cagttgtata ttgaccatcg
     8401 ctgcgatcag ttctgatacc gcacggaatt cattgagttt tctgatcccc gatggccccc
     8461 tttcggtgcg ttcaacggcc cctcaccgaa ccgttccaca gcgctgaggt taacgaattc
     8521 gaaacaattg gatcgcccta tgacggggtg tgttcgtttt ttgatttcac gttccaagcg
     8581 tcctgaacgt caaacctcac atgcccccaa gatggaactg gttgaatgac attcctacaa
     8641 tcggaacgcg atggcattct ggatgacgtt tcagtcgtca gacggattgc gcgggtcggc
     8701 ttcaggattc cggtgttcgg tccagccctg cggtgggcgc tcctgagtgc tttgcggacc
     8761 gtgcgtgagg tcacgagctt ttcgcggacc gcagatttcg tggtgcgtgg ttcgctgacc
     8821 ttgctcaaga cgacacggcg attgccggga ggcaggacgc tgtttgatcg cgtctggctt
     8881 ccgcttgcgc gcggcagcgt cgtgctggcg gaagcccccg agcgtcccct ctcggatttt
     8941 tccccgcttc tggaacgtga cgcgttttcc ggcggaccga tcgtgctgtg caacaacgca
     9001 ctcgcctggg gcggcgtcga gcggcagatc gtcaataccc tgaacggcct cgcggcagag
     9061 acgacggaaa gcctcggatt gctgtgcgtt cgtctcgacg ccgggccgga ttacggcttc
     9121 tacctgccat cgctggacgg gttccgaggc tttgtccgca atgcggttac ccccggagaa
     9181 gccgctgctc gcatcaacgc gatggttcgt gctgaccggg cgggcgatct gtctgctcag
     9241 atcgcatggc ttccctcgca tgttcaggag gaggtcaggc gctttgtcgg cgaattcctg
     9301 acactcaagc cgtccgttgt ccacgtctgg caggatgccc tgtccatttc cgctggcatg
     9361 gcggcgaagc tcgtcggagt gccgaagatc attgtggcga gccgcaacat ggccgcgcgt
     9421 cggttcgcct accatcagcc ctacatggcg accggctacc gggagctggc cgcctgtccc
     9481 gacgtcgtgt tcttgaacaa cagcgaagca ggcgcgcggg actatgcggg ctggctcggc
     9541 atcctgcctg atcgcttcga actgctgcgg aacggcgttg atgaggcctc tctcagccgg
     9601 ccgccctcgg aggctaccgc cgcactcaag gcccgtctcg gaatcccgcc ggatgcaccg
     9661 gttctcggtt cggttttccg attctacgac gaaaagcagc cgatgctctg ggtccaggct
     9721 gccggcatcg tggcgaaagc cctgccgcaa acgcacttcg tggtcttcgg cacaggaccg
     9781 ctgatgggag aagtgtggaa gttcgccgaa cggcagggct ttgccgatcg cctgcacctg
     9841 ccaggaacga tcgaggatgt tgcgctgggg gtgtcgctgt ttgacgcatt catgctgacg
     9901 tcgcagcttg aaggcacccc gaatgtggtt ctggaggcca gcttgctcgg tgtccccgtc
     9961 gttgctacta acgccggcgg cacggtcgaa gcgattgcgg acgggctgac gggttatgtc
    10021 gcccgacatg ggcaggccga ggcattggcc gaactggtcc tgaaggtcct gcgggatccg
    10081 atttggccgg cgcgggccag ggaacacggc ccctcattca tctcgcagcg gttcggcctg
    10141 aagcggatga tcgacgaaac agtcgcgcta tatggcctga agcgcctgaa gtagaggtct
    10201 tttgtgtttt cggtcgaaca tctcaacgtc ttgcgtgctg cggaaatcac caagattctc
    10261 atgcatctcc ggccggaagg ggcccgcatg ctggaaattg gcgcaggcac gggacgacag
    10321 gcacaggaac ttgcaagcaa gggattcgcc gtcgaggcga tcgaaatcct tgattcgaac
    10381 tatgccgggg accggctgtt tccgatcacg aactatgatg gccgcaacat tcctttcgag
    10441 gatgccagtt tcgacatcgt cttttcctcg aatgtgctgg aacatgtgcc tgatctcgtg
    10501 cagatgcatt ccgaaatccg gcgcgtcctg cggcccggcg gctatgccgt ccatgtcctc
    10561 ccgacgcacg cctggcgctt ctggacgacg ctttcggcat ttccgacagc cttccagtat
    10621 gctgcaacgc tgggtggcca actcgcgccg aaatggccct tcggcccttc cgaaatcaag
    10681 cgcctgatca agatctggct gatggcggcc cggcacctgg ccgcaccgtt cttccaaaga
    10741 cgccatggcg agcgcggcaa catcctgtcc gaaacatggc ttttccatcc gagctggtgg
    10801 agacgcaact tcgcccagaa cggcttcagc atcatgcgcg acgagccgat gggcctgttc
    10861 tacacgggca acatgacgtt cggccagcgc tggtccatgg agaagcgtgc acggctggca
    10921 aagacactcg gaagtgcctg ccatatcttc gagttgcagc ccacccccag gaaatagccc
    10981 ccggcagcgc gccgcgcgtc agtgaacaga ccggccaggt cggatcaagt ccgtatcgcg
    11041 tccggcggac cgacttgcct cagggagcgt tcccgggtgc ggcatgtcgc gcaccgacat
    11101 cagcgtacag ggcgattgtc tcgtcgatca tgcgctccaa cccgaaggta tcgagaatga
    11161 acttcgggcc atgatgcccc gcgcgggcct tgaacccggc atcttccagg acatccagaa
    11221 ccttcccggc aatctcctgc ggatttgcgg aacgcacgac gaatccgctt tcgccctctg
    11281 cgatcgtttc cgcagtgcct ccggcgtcgg tcgcaacgac cggcacgccc atcaggctcg
    11341 cctcgagaac gacgttcggc gtgccttcca tgcccgaaca gagcaggaag acatcaaaca
    11401 gcccgagccc aagcgttggg gcggcaatgg tcccgggcaa atgcacccga tcggcaaaac
    11461 cgagttctct tgcccggcgc agcatgtcct cgcgctgggg gccggtgccg aagaccacga
    11521 aatgcgcgtg cttgagcctg gaggcaacga tacccgccgc ttcaatccag agcagcggtt
    11581 gtttctcggc ataaaaccgg aagatcgagc cgatcaccgg cgactgttcg ggaatagcga
    11641 gttcggcgcg aagacgggca acatcaccct gcgaagcagc aaccagggca ttcgggtcga
    11701 tcccgttgcg caggatgacg aaccgcgcct ccggaactgc gagccatgcc gcgtaggagc
    11761 gcgcgccggc gacgctgttg ttgagcatcg tcacgtcatc gcagccggcc agttcctcat
    11821 aggcgaggcg catgtgcggc cggtgatagg cgaaatggat cggcgcgatg ttgcgccccg
    11881 ccacgatgac cttgggcact ccggccagac gcgcggcata ccccgcggaa atggacaggg
    11941 aatcctgcca ggcatgaacg acgcgcggtt tgaggcggac gaaatcggcg acaaaccgcc
    12001 agatgtcctc ctggacgtcg cgcggcagcc agccgatgtt ctgctcgatg tcgcggcggc
    12061 gttctgcggt catcgtctcc gcaaggatcg cggatgcctc gctggcgggg acgacgttgc
    12121 gcaagagccc gccaaagtcc tgtagcgacg gccggtagaa gtcgtaattc tcgtcctggc
    12181 cgaggcgcag gcacaaaagg ccggcgggct ccggcaagcg cgcggccaag ccctgaaggg
    12241 tatagacgat ctgccgctcg acaccgcccc aggccagggc gtcgttgaca aggagcggcg
    12301 ccccagtgaa ccgatcagcc ggcaggaggg ggctgatgct ggcgagcgga cgcgcccgcc
    12361 gtctgaaggc ctccattctg gaagtagccg tcgcttgctc gatcaagctg gcgacaccgg
    12421 caagccgggc acgcacgctt cggggcagga cacgccgggc caccggctgg gcaagcgaaa
    12481 aagcatgatc cagcatgcga cgcagggcga acccgagcga cgggcgccag ccggcattcc
    12541 caccggcaac gcctgtcttt tcccggccct gcgctttgcc caggtcgtcg cgtggtgcag
    12601 tcacgatgat agcgctcctg tttggttctg ctggcgcggc aagaaccaaa gcctgcgcca
    12661 gaccaacggg gtcctttcaa cagtcacggg tccgaaaagg caaggcgagg accgcgtgcc
    12721 acgatagcgc acctcgccag ttcaggctcc aacttgcctg tcccaacggc gcagcgcctg
    12781 ctccagcgct tccaccgtgc gtcgcgccag gcgtttctgg tcataaggag cgatctgctc
    12841 ccgagccatt cctgacagaa catccctccg gtcgaggacg tccgaaaggg catcggcgag
    12901 aatttccggc gcgccgggcg gcacaatgcg gccgcatggg ccaacgatcc gcctcgcatc
    12961 gccgacatcg gtcgccacca ccggcaatcc gctggccaag gcctccgcga tgacattgct
    13021 gaatccctcg ccgtaagcag aggacgaaca ggcgacatcg aaccccgggt aaagactggc
    13081 gatgtccgcg gccgccggga tgacgacgac gtcttccccc agaccaagcg aagcagcctc
    13141 cgccgtgagc cggtcccgct gctcctggct accgaccgca accaagacgg ccttccagcc
    13201 cgtgcgctgg tggcgcagca gggccagcgc ctgcaaaaaa ccggagtgat ccttcatagg
    13261 atcaattctg gcgacgtgac cgataacagc cgtgtcatcc cgacaattcc aggcgactcg
    13321 tgcggtgttg cggcgatgat cgatcccgtg ctggtgcggc gcgaagcgtg ccaggtcgat
    13381 accgttctct atcaccacga tgcgccggtc cgggacgccg cctgcacgca acacctcaag
    13441 gccagactgg gaattgacga tgagcaggtc ggcaaggggc atcaagcggc gctccagcca
    13501 ggctgcccat cgccacttga tcccgtagtt ctccatgcgc atgtcggagg cgcgaatgcc
    13561 ccagaccagc agcggccgcc gggtgagtgt gcgggcacac aggcatgcca gattgcccgc
    13621 cgtgagatag ccatggacga catcgggccg ctctctggcg atcaggcccc ggagactggc
    13681 gatgcgcgcg gccgcattgc ttccggagag cacctccatg gccacgcccg tggccaccgc
    13741 atcggcctgc atggctccgc catcgcgaaa cacggcgacc cttgcctgat ggcccaggtc
    13801 gccaagcccg cgcgccagcg ccagcgcctg gcgttcagcg ccgccgaggc caaggcgccc
    13861 aaccacgatc aagaccttca tgacgcgtgc gataagcgtt gtggcaggcg cgggcaatag
    13921 gctagagcgg tgcccagccc cctcgcacac tgctccaggt cctttgggcg gctctggaat
    13981 cctgcaaacc cggcaatgcg catccttcac gtcatatcca acctgatgtc gggcggcgcc
    14041 gaaagcatgc tgaccaaggt cgtggaagcc ttgccggctc cgcgcttcga gcacgccgtc
    14101 gtgacgttcg tcaatggcgg cctgttcgcc gatcgtctgg gcgaggccgg agttcccatt
    14161 ttcggtctgg ggcagcagaa agggcaggca gcgcgtgcct tgctgcgact gtccgatgtg
    14221 cgccggatcg tcgaagagtt tcagccggat atcctgcagg gctggatgta tcacggcaat
    14281 cttgctgcct ccgtcgcggc gggccgacgt cctgtcatct ggaacgtgcg tcagcgcctc
    14341 gaacgtctgt cggataacgc cgtcacgaca cgccttgcca tcctggggtc cctgggctgg
    14401 cgcgcgcgcg tccgcgccgt catctacaat tccgagcatg cggcgctgga gcacgaacgc
    14461 tggctttacc cccgggcgcg gcggatcatc atccccaacg gtttcgatct tgaggcgttt
    14521 cggccggatc agacggcgcg cgcgaccctg cgcggcgagc ttggactggc gtccgacgcc
    14581 gttgtcgtcg ggcgcatcgc gcgcgacgac gcaatcaagg acacaccgaa cctggtgcgc
    14641 gcgtttgctg ccttgccgtt tcgcgatgtg cacctggtcc tcgccgggcg tggcatggat
    14701 tcccggaacg ccctactgct cgcagagcta cgatccctcg ggatcgggga ccgcgtccac
    14761 cttcttggcg aacgccggga catcgcgcgg ctgaacgccg ggttcgacct tgccgtgctg
    14821 tcatccagtc acggcgaagg ttttccgaat gtcgtgtgcg aggccatggc agcgggcacg
    14881 ccgatcgtct ccaccgacgc cggcgagtgc cgcgccatca tcggcgatcc cagccgtatc
    14941 gcgcccattc gcgatccgca ggcccttggc gccgtcatga cgcgcgtact ggcgttgacg
    15001 tccggcgaac gcatggcact ggggcgcgcc gatcgcgagc gcgtctcgcg cctgtacagc
    15061 ctgccggcgg tcgccgagtc ctatgccgcc ctctggcaca ggatcgccgg aaaacaggcg
    15121 taaacgccgg ttcgccgctg ggccagcccg ttcaggcgcg tttctcgaac accaggaaca
    15181 cgcgaaaccc gacatagcgc ccgatccaac gctcgacgcg ccgttccagc gccatcagcg
    15241 cccgtgccat gccgcctgtc agcagggtcc atttcttgaa cccgcccgac aggggatagg
    15301 acaacggcga gaaccagacg acctcgcgca cagtcatttc gggcacgagg tgctcgaacc
    15361 gctcgcgatg ccgcgtggca atcagcgacg ggatggcctg gttcgattca tacgggttcc
    15421 gtgcgggatc aggcgctccg gccgtgagcg gatcggcgct catgatcacg ggctcctcgt
    15481 gaatgtagtt gaagaacgcc caggacagcg gcgttatccc gggttcgacc atcacgaccc
    15541 gtcccccggg acgcaacgtg cgcgcggcct cacggaagaa gttcagcgga tgctcgatat
    15601 ggtgcagcac gtcgaccatg acgatgttgc ccaggcaatc cgccttgaac ggcagtttct
    15661 gcgcgtccgc gaccagatcg agccaggggc cgaactggat gtcggagcca atgacgtcgg
    15721 gcagcttttc cggcaggttg ccgatccccg cgccgatctc cagcgtcggg ccaggcccgc
    15781 aggcggcggc gatgcggtca tagaagtcgc cgtagacggc ccgaagaacc ggctttgcct
    15841 tccagaccgc gagataccgg ttgagcgcct ccggggtgag atcgctctgc atgtgggatg
    15901 cctgttgctc gcccatgatc acaatgcctt gatgcgcatg aaagcgaaca ggaccattcg
    15961 cagcaacatc acgccgtgcc ggaaccggga gatctgcgtt tcgccgtagg agcggttggc
    16021 atagcggatg ggtacttcaa gcaccttcag gttgagcttc gaggcgccga atatgagatc
    16081 gaagtcgccg aacggatcga agtcgccgaa ataggcgcgg cccgccttca ggcgctggta
    16141 gtcggcacgc gagatcacct tcgtgccgca cagggtatcg gtgaaccgct ggttgagcag
    16201 ccaggagaac agatacgaga agctcttatt ggcgatgagg ttgagaaacc gcatcgcctc
    16261 gtcttccatc ggatagacga ggcgcgagcc gttcacgaac tccgccttgc cgctcgaaat
    16321 ggcatgccag tacttcggca gttgctccgg cggcatggtc aggtccgcgt ccaggatcat
    16381 cagcacatcg ccgcgcgccc ggtcaaatgc gtggaagacc gcatccgcct tgcccttgcc
    16441 gggctggcgc atgatcttga tgtcatactg gggataggcc ttctggaccc gctcgatctc
    16501 ctcccaggtg ccgtccttgg aatgtccttc gacgaagatg atctcgagat cgtcggtgaa
    16561 ccgcggcgtt cgcagaacgg ccgcttcgat gttgcccttt tcattgcgcg ccggaaccac
    16621 gacggtggcc gaccggtagc ccggatcggc acagcgctgt gaacggcaga cgctgtagtg
    16681 gcgcaacgcc agattacgaa tgccgggaaa tggcgcgatg aagcggttca ggaagcgccc
    16741 cagacccagc agcctggccg ggctgagcag gcggatctcg ctcttgacca gttcgaagtc
    16801 cgagagttcg gccagcccct tcacgtccgc gacagacagt gcgttgactt ccggcacctt
    16861 ggtgcgcagt cccgcggttt ccgcgagttt cagcacgggc caccacagat gcgagaaata
    16921 gccgatgatg acgcgcgtat cgcggttgca gagccgatga agccgcgcca ggaaagcctg
    16981 gcaatcgtcc atctggccaa tgctgtcgac cacgacgatc gcatcgaagg ggccctcgat
    17041 cgcatcaagg gtagcggggt cttcggcatc gcccaccacg aattcaagtc cggggtgcgc
    17101 cttccgcgcg acgtcaatca tgcggggcga aatgtcgacg cccacgccac gcgacggctt
    17161 gagagcggca agaaggtcgc cggtgccgca gccgatttcc agcacccggg caccgggcgg
    17221 aatgagaaac tgaaggtagc gcaggtcttc ctcgtgaaag aaggcgttgc gctcgcgcca
    17281 agcgcgccgg tcgggagcgg ctgcatccga agcggcccga atcttttcct tccgcggact
    17341 aagcggagcg gcagccaaga gcgtcgtcat gttcacattc cagtcttttc ggtctgatct
    17401 gtctgcggcg cgcgctgggc gctgtcgctt tctgctgccc gcatagaggc attgcgtcga
    17461 aaaagccaat acagcgcccc tggcactgtc gccagcatga ctgtcattcc gaacgcaacg
    17521 gagaatacca cggcctcctc ggccgaggct ccgtgcgccg tcagcagcgc ggtgaccgcc
    17581 agctcacgca cgccccagcc tcccaccgag atcggcagca gcgtgatcac catgatgtag
    17641 accatcagca cggcggcatc ggcaagcggc agcgccaggc cgatggcgcg gccgagggcg
    17701 aagatgcaca agatcgtcag tccgtggacg agcgcgctga tcgcaaacag cttgacggcg
    17761 ttcggcccca ggagcgcggc atgcgtctcg gtcagcgcta cgccaagaaa acgcaacatc
    17821 tgccgtcttt cgagcatggc ccccagagga cgcgccgaaa gcagcgccag cgttccgccc
    17881 atcgcaacca gcgccacggt cagcaacacg ggctggcggt acccgccgag cgactccagg
    17941 ccggacggca gcaacaggaa cgcgaaagcc atcaggaaca gcgaatgaac gccgagcgcc
    18001 cgatccagca gcacactcca tgccgcatgg gggagcgtca cgccatggct gcgcaacatg
    18061 agcgcgcgaa caccatcacc ggcaagattg ggcagcacct gactgacgaa ctggccaaac
    18121 ccgttgaccg catgcacatc cccatgcggc aactggtaca tgccgcggcc gagggaggtg
    18181 acgaccagtc gccaacgctc tccaaccagc ggcacctcca gggcgatcat cagaatggcg
    18241 agggcaaccc agccgggctc caagttttcc agaagctttc gcagattggc cggatcgatc
    18301 tgccgcgaca ccatgaccat gcagccgacc gcaactgcca gcttgaaggc gacaagaccg
    18361 cgcttgcgcc aactgacagc ggcgtccttg gcagtcgcca caggttccga cctttcacct
    18421 gtcaccgctt acaggctcca ctgcaaactc caaatccgtt cttccggcct gtccaggcca
    18481 atcccgagcc atgcgccgca ggcggcttcg gctgaaggcg cccggccacg caaatcctcg
    18541 cttcatggcg ggcgggccca aggcaaggac cgctgattcc cgacacgatc cgcgccaatg
    18601 gtcgcggcac aagagcggcc gatgcggatg gtccggtgtc gccgagcggc gatccttcac
    18661 accatccggc tttgaccgtc cagaaatgcc ttgtaggcgc gctgcaatcc ttccgtgagc
    18721 gacgtgcttg cccgccagcc gagcgattga agccgggaga cgtcgagcag cttcctcggc
    18781 gtgccgtccg gtttgctcgt atcgaaaacg atctcgcctt cataaccgac cgttcgcgca
    18841 atcgactcgg caagctcgcg gatcgtgacg tccgtgccgg tcccgatgtt caggaggcgc
    18901 tcgtcggacc aggacttcat caggtgcacg caggcatccg cgaggtcatc gacaaacagg
    18961 aactcgcgcc ggggcgtgcc gcttccccag acaacgaccg accgcgcgcc actctgcttc
    19021 gcctcgtgga agcggcggat gagtgccgcg gcgacgtggc tgttctcggg gtgataattg
    19081 tcgcccggac cgtagaggtt cgtcggcatg accgagacga aatcgtcccc gaattgccgc
    19141 cggtagctct cggccagttt gatcccggcg atcttggcga tagcgtaagg ctcgttggtc
    19201 ggctccagcg gcccctgcaa caactggtcc tcgttcatcg gctgaggcgc gagcttggga
    19261 tagatgcagg aagaaccaag gaacatcagc ttctcaacgc cgttgagatg agcggcgtgg
    19321 atgatgttca tggcgatcgc gagattgtcg tagatgaagt ctccgcgata agtgttgttg
    19381 gcgtggatgc cccctaccct ggcggcggcg agaaagaccg cctgtggccg gttttcagcc
    19441 atccagctct cgacctcggc ctgccggcga agatcggcag acgaccgtgg cgcgaccaga
    19501 atcgtgcagc cctcgttcgc gaggcgccgc accagggctc caccaaccat gccggtgtgg
    19561 ccggcgacga agacccgttt gcccgccagc gcgtagttag cggacgccat cgaccctctc
    19621 cttcttggcg gcggtgaggt cggtggcgac catgtcccga accatcgtcg cgaagctgat
    19681 ctccggttgc cagccaagcc gctgctgcgc cttgctcgcg tcgccgacga ggaggtcgac
    19741 ctccgtcgga cggaagtagc gcggatcgac gcgcacgagc gtcttgcccg tcttggtatc
    19801 cacgccgctc tcgtcgacgc cgctgccccg ccactcgatc ttgcggccaa cttccgagaa
    19861 cgcgatgtcg acaaattcgc ggacggtgcg ggtttcaccg gtcgccagca cgaaatcgtc
    19921 cggttcatcg gactgcagga tcatccacat gccgcggacg tagtccttgg cgtggcccca
    19981 gtcgcgcttg gcgtcgagat tgcccaggaa caggcaatcg tccagaccgg tctcgatcct
    20041 ggcgacggat cgggtgatct tgcgggtcac gaaggtctcg ccgcggatcg ggctctcatg
    20101 attgaacagg atgccgttcg aggcatacat gccataggct tcgcggtagt tcaccgtgat
    20161 ccagtagccg taaagctttg cggcggcata gggcgagcgc ggatagaacg gcgtgcgctc
    20221 gcgctgcggc acttcctgga ccaggccgta cagttccgac gttgaggcct gatagaaccg
    20281 cgtctgcttc tccatgccaa gaatgcgaat agcttcgaga aggcgcagaa cgccaatgcc
    20341 atcggcgttc gccgtgtatt ccgggctctc gaacgagacg gccacatggc tctgcgcggc
    20401 caggttgtag atttccgtcg gctgcacctc ctgcaccaac cggatcaggt tggtggaatc
    20461 ggtcatgtca ccgtagtgga ggtgaaaccg cgcgctttcg acgtgcggat cctggtagag
    20521 atgatcgatg cgttgggtgt tgaacgagga tgaccggcgc ttgatgccgt gtacctcgta
    20581 gcccttcgaa agcagcaact cggaaaggta ggcgccatcc tgtccggtca cgcccgagat
    20641 gagggcaacg cgccgcgcgc cttccgccag cgtcatacta cgatcctttg cggcagttaa
    20701 aaaagggctc tggctgcggc cttaccgaaa ccggaaaggc gcatagcaca attctgggag
    20761 cgcctgtcaa tgcgcgtcgg ccggcgccga tgcccggacg acgtgcgaag ataggataat
    20821 tgggcctatc tcgtgatcag ttgggccggt tgcaccgaac gaatttcaac cacctccaga
    20881 ccgccgatcg aatcggaatc ggcgtggccg atcgtggcga cgcaagcgct ccaatagcgg
    20941 ctctcccgcg gtatgctgac cgccttgggg aatgccgcca gcaacggata gaccgcccgg
    21001 gggttctcag gtttctgcac ctcggcgacc gcgcgcaggg tgtagggaac gctcgccttc
    21061 gccagcgcct gggcaaagac tggtcctatg atcttcgtgg cgtgcaggag ttcggattgc
    21121 cgtacggtcg gtgacagcag gaaaccaggt tcggaatccg cccggacatc gaagatgtcg
    21181 gcgaagatcc tgccttccgc atacaatcgt ctttcgccgg caggacaggc atccagcgcc
    21241 gcgatctggt cgaccggctt cagcttgccc ggaccgatga tcaggggtgc agcagcgatc
    21301 aatgcgacaa cggcgaacgc gccgacaccc gccagcgaca agcgccttgt caccgcaggt
    21361 tccatggccg gcggcagcgt tggcgaagtc agcgtgcgcc agagatcgaa agcgccagct
    21421 ccttgcatca ggttcgccaa gccgcggcgg aaggcatcaa agcccagcgc gacgagcagc
    21481 cccatcaagg gcacgccggc cgcgtaaacc cgcgtccccc cgtccgtcag gagcaacggc
    21541 gacgaaagca caatgccggc ggccgcggcc agcccgatca gcgccaccgg gttccggaat
    21601 gacacgaggg cgatcaacgc gccgaatatc agccagatgt tgatcaccgt caggtcgaaa
    21661 tatgtcagga agaactccgg cgtccgaagc aactcatcca tcagggcctt gacgatcagc
    21721 gacggtttcg cgcggatagc ctcgatcgca aaggcgtaga tcgccggctc cggcggcgtt
    21781 ttcatggtgc tcgcgaaaag ctgggggtga tcgacaaaga tctgctgcca gcccttgccc
    21841 cccacggcca gcccatagag cgtataggag aaattgctct ggatgaggcc ggacgagcct
    21901 ccgagcattt ttacgagata cggggagtgg gcaaatccaa gcacaccgcc cacgccgccc
    21961 gcgacaagcc aaagcgccat ttgctttctg ccgcgccgcc attgattggc ggcacccgcg
    22021 aggatcacca gcggcaacag gaagaacggg cccgcacgcg ccatcagccc aatggcgaga
    22081 accatgacgc ccagcccgaa caatgcggcc tggtcgcggc gcaccgccgc caggatcgcc
    22141 gcgagcccga acgcgcccat ggcaaatccg gtgctttccg aaagggttgt cggcgtccac
    22201 tctgaaacga acgcgccgca ggccgtggca aaggcaagcg ccgcccagcc ccccagggcc
    22261 agcatgatct cacgcgtggc caaggtcagg cccgcagcca cgagcaaggc gccgagcccc
    22321 atgatgacct gcatgtccgg agcgcccgcc agcatgcgca gggtcgtcag gacgccggtc
    22381 agcggacggc gcgaattcca gtcgttgagc gtgccagtcg tcagcaactc gagcgcgccg
    22441 tgcagatacc caaacgagtc cgatgcctgc atcgagccaa gaatatggaa cccggtctgc
    22501 gaatgggtat tgatccgtcc gctggcgatc caggcgtcgg ccaaacccgc gaacggcatt
    22561 gcgaaggctg ccagcaaaat ggcgacggac gcggccgggc cgggccgact gcggacatcc
    22621 tgcaccaaga cccgccaggg caggcaggac agcacgagca cggcgccggt cagagttatg
    22681 gcctgcgtca gccggagttt gagagtaagc gcggtttccg tggctggaaa cagcgaagaa
    22741 ggaatcagca tccccgcaag tcccagcacg aacaggatcg agcctgcgat catcgcgcgg
    22801 cgcaggtccg gtgccttgtc gacagcgacg gcaaacccgc tgcgcaacgc cagattgctc
    22861 accagcaccg ccgacagaag catcccaagg agcagtaggg tgatcgccct gtcgaaccgc
    22921 agcgaaccgt tcaccgtcat gccgttctca ggcgcggttg ggcctcccgc gttgcgatag
    22981 gagaagcgaa aggtctgcga aagcgccgaa tacaggcccc cgggcatctg ctcgatgcgg
    23041 gcatcggact ggccggaagt cagcggtccg ttcctgtcct gaaggatcgg gcgcagcgag
    23101 agggtccgtt cctcgtcggt gacgaactgg ctgaaaagcg gcaggttcga cacgtcgtag
    23161 gtgtacaatt gtcccttcac gtgctgcacg gattcgggct tgacgctgtg ccagcccatc
    23221 agcgttcccg aggtcacgag cgccaggagc agcgccgccg ctatgccaac ggtacggatg
    23281 atccggctcg cgacataact gctgcgcagg ctggacaccg gaaaggccag cgccggcgtc
    23341 agaatgctgg caatgcccag caagacgaga aagaagggca agtcactgag ggttgcctcc
    23401 tgcaggcgca accgcgtcac gacaggcgcc aaagcaatgg cgacgccggc gaaaaacgga
    23461 agcacggcaa gcaaggccgg ggtcctgtcc gctacgccgg cggatgcaga cagcctggac
    23521 gcaccgcgac gcaacacgtg gccgatgccg agtcctgccg ccaacaggcc cgtgaccagc
    23581 agccagtgct gcgacaccgg cgcaacaggc ccggtcaacg tgtatgcccg gccattggtc
    23641 cgggggtccg tgttgtccga cgtggaaaaa tagagatacc tggcccagac cgagtatcgc
    23701 ccgttgccga tggtgcgaat gtcctggtgc agcgatctcg gtgcgccaag acgcaggtca
    23761 ttttccttga gaacgctgaa accgacgagg ccatatcgct cgtcgacgta gaaatagggc
    23821 gttcttgcca ggcccagcga cccgatgaag gctttgccga tttccgcctg gtagcgggtc
    23881 atgtccagcg tgctgcgcac accgacgaag cctgacgaca gcaccagcca ggcggctagg
    23941 atgcctgcgg caaagaacgt caagacacgg ctgcccagcg tcgcaaagcg aattatcgtg
    24001 gcatttggcg tcatgtcttt gtctcgcgga tgcttgggct ggagcagcgc accaccggag
    24061 gtcgcgccat tgcatcttct ccccggcata gcaagtgtcg cacgccccgc accgttccga
    24121 cgaacgcgca atgcgagcat cacgctgctg cgcaggcatt gccggctcgg gctcgcaggt
    24181 tgccagagcc agggcgagcg actagagaga gcccaaaatc gtgatcccct ggtcggacca
    24241 acgccgagac ggcgcatgtc gcgacctcag ctttggaaat caggcgttgc cagtcgtcga
    24301 gctttgctcc cgaatgaacg aagtccccaa aagcctgggg ccggcgttga tccttgtctg
    24361 ctcctatcgc ggcgccgccg accgcaccgc cccgtcggat ctggtcggct ggaagaggac
    24421 gctcgtcgac gccgtgacgc tgcgaaagct gcgcgagtat gtggcgacct ggcgggattt
    24481 ctacgccaat cctcggaatg ccgcctatca gcgtcgtctc gccgaggccg tcgctgcccg
    24541 ctgtgcaccc ggttcgagca tcgtcgtgga cgctcgcttg gcgcccgagg ccgtgcctgt
    24601 gacgggcgtc gaggcagatc gggtttcgcg tcgcgatctc ggcgctgaaa aggcggcaga
    24661 cctgccgtcc gcgccggtcg ttgtgtttgt ctatcccgat ccgctgggtc tgagctggca
    24721 aaggctggag gcggcgatcg ccgcccaggg ccgcagcatg ttcatcgtca atggtcgcgg
    24781 acgcctgttc aggctcgaca ggcaagcagc tcgcgcgctt cgattgcggc ggttcatggc
    24841 cacgacgcgc atcgccgaac tgcttgcggg acttatgctt gtgccgatct ccggaatttt
    24901 cgccctgcgc gaccgcctga acggtcggac atgaccatgc tggacaccga aaaagccgac
    24961 cccgcgtcgt cacgtgcaac cgtgatgaag gaaaagattc gccgttggtg ggccgaagcg
    25021 cccatgacct atggcgaaac gcatggcaca accgagtacc acctgccgga tggcagcgtg
    25081 gagacggtcg aaatcggcac caggcgattc tttgaactgg cggatcagcg gttctacagc
    25141 tggaacacgc cgctccatca gccagatgga ccgttttcgg cgattttcga ttacaagcgg
    25201 ttcgagggca agcgcgtgct ggagatcggc tgcggcatgg gctgcatggc gatgaactgg
    25261 gcgcagcgcg gagccttggt gacggcggtc gacctgaacc cgacttcggt cagccaaacg
    25321 aagctccggt tcaagcaatt cggcctcgac ggcgatattc gtgaaagcga tggtgaaagc
    25381 cttccgttcg aggacgaaag cttcgactat gtctactcat ggggcgtgct gcatcatacg
    25441 cccggaacac gcgaagccct cgccgaggtc tatcgggtcc tcaaaccggg cggccagttc
    25501 ggcttgatgc tgtatcatcg caactccttc ctttaccgct acacgatcgc ctgggtggaa
    25561 ggcttcgtga acatggagag ccggttcctg tcgccgcttg aactcgcgag ccgctattcc
    25621 gacggcgcca gggaagatgg caatccgcat acctggcctg tgaccaagcg ggaagttcgg
    25681 cgtgatctgg tttcccagtt tggcggcgtc gacatcaagg tgctgggcac cgacgtgccc
    25741 gtcattctga acgtctggct gcccaggctg gccgagaagc tgttgccgtt gtcctggcaa
    25801 aaggccatgg cacgccgctt cggctggagt ttgtggatca caggcggcaa gagcctatga
    25861 tgacggcgtc ctcgctgacg cggtttgcca ggagggtgcg ctatgccgtg gctcatccca
    25921 cggcggtcgt gcgctatctt gtgggaacgc cggcggcccc tgcgatgggt gcaacggcag
    25981 tcgcagagga tgccccccct gtcgagttgg cgcagcccga tcccttgcag ccattcgaac
    26041 gctatctcgc acttggccgc gcccagggaa tcgaacagcc gacgctgttc ctgtcattcg
    26101 actgcgatac cgacctcgac ttcaccgccg cgctggaggt gaagcgattt ctggattccc
    26161 tgggaatcaa ggcgacctgg gctgtgccgg gcgtccagat cgaacgcggc gcggatacct
    26221 accggtcgat cgcagcgacc ggcgcggagt tcatgaacca tggtcagttg ccccataccg
    26281 cctggggcga ggatcgctac atcagcatca cctggtacca tgaaatgacc ccggaggatg
    26341 tcactgcgga cattctggcg ggcgacgcca cgatccggcg cgtgctgggc gtcacgccag
    26401 gcggatttcg cgcgccgcac ttcgggcatt atgcggcgcc tgaccagctt gccctggtgc
    26461 acgcgtgcgc gcgccagctg ggctactcct actgttcgac caccctgccg gcctatggtc
    26521 tcggcaacgg cccgcttcac gacgccgacg gcattgtcga actgccgacc ttcggctctc
    26581 tcgtcgcgcc caccagtgtc ctcgattcct ggagctatct gactgatcgc cgcgtgtatc
    26641 aactcggcga cgtctatgcg gagctgatgt gcgcaacggc ggagcggctg gtgtccgagc
    26701 gcgccgctgc attgctgacg tggtatgctg acccttccca tgtggtcggc caggctccgt
    26761 tcgagcgcgc catgcagcgt ctggtttccc tcggcattcc cagcctccac ggcagcgaag
    26821 ccgccgctct gctgcggcgg gcatcgccag gctgaactga caggcttgcc atgtgcggaa
    26881 ttgcgggttg gctggatcag gaacgccgcg gtgatgcggc cgatctggcg cgcgtccgtc
    26941 gcatgtgcga cacgatggtg catcgtggcc cggatgatga cggctatatc acgctgcctg
    27001 gcgtggcgct cggaatgcga cgactggcga tcgtcgacat ttccggcggt caccagccga
    27061 tggcctccga cgatggcgcg atccagatcg tcttcaacgg tgagatcttc aaccacgatg
    27121 ccctgcgatc ggagttggcg gccggcggca cacacttccg cagccgcagt gataccgaag
    27181 tcatcctgcg tctctacgag cagttcggcc tggcagcgat cgatcgcctc aacggcatgt
    27241 tcgccatcgc catatgggat ggccgggaca aggccatgca tctcgtccgc gaccggatgg
    27301 gcgagaagcc gctctattat ctctgggacg gtcgccgtct gctgttcggt tcggagatca
    27361 aggcgatcct ggccgggctg gaccagcgtc cgcctgtcaa tccgcaggcg ctctgggact
    27421 ttttcacgtt tcgctacgtg cctccggatc acacgatctg gaagtccatt tccaaactgc
    27481 cgcccgcgca ccgcctgacg atacgcgccg ggggcggcgt tccggagatt gcgcgctggt
    27541 gggacattcc acggccaagc caccgccagc gcagcgacag cgacgaggtt gcgcagttcg
    27601 atgcccttct gaccaacgcg acccaactga ggatgctggc ggatgtcccg gtcggaatcc
    27661 tgctgagcgg cgggctggat tcgagcgtgg tcgcggcgaa ggcggccctt gaggggcagc
    27721 gcgtcaagac cttttccgtc gccttcgaag gtgcggaagc gatcgacgaa cggcgctatg
    27781 cccgcatggt ggcgaaggca ttcaacacgg accaccacga ggtggtcatc ggagagcggg
    27841 aattcgtcga tttcctgccg gacttcgtgc gatttaccga cgaaccgctc gccgatctcg
    27901 cctccatacc gctgcactac gtgtgcaagc tggcgcgcaa gaacgttacc gtggtgctgt
    27961 caggagaagg ttccgacgaa atcctcggcg gctattcctt cgacaactgg gccgcgcgct
    28021 gggatgaagc tgctaccttg tccaagccgc tgaccctcat ggagcgcctg ctgcggcgcc
    28081 aggcgccatg ggtcgatccc ggcctgctcg acctgcggga cgcaccgcag ccgctgacca
    28141 tgacctccta catgacgagc gaggagaagc gcgccctatt ccgagcaggc atggaggcgc
    28201 ccgacagcct ggatggcctg cgcgccaagc tggcgcattt cggcaaggcg tcgccccttg
    28261 cgcaggccct ctacatctat tgccaggact ggttggtcga agacctgctg atgaaggccg
    28321 acaagatgtc gatggccaca tcggtggagc tgcgcgcgcc cttccttgac caccgcgtcg
    28381 tcgaagccgc cgcccggctg cccgaccatc tgcgcgttgg ccggcgcgcg gacggaacat
    28441 acgaaacgaa gcggatcctg cgcatgctcg cggcgaagtg cgcggtgcct caggagatcc
    28501 tgacacgccc caagatgggc tttccggtgc ccgtctacgg atggctttcc gacagtctgc
    28561 gaccgtttgc ccacgatttg ctggcgtcgc ctggaacacg cctcaacgat tggtgcgatc
    28621 ctgccgctct ggccgccatc cttgcgcgcg gcacggcgcc ggacgcgccg atgcaggatc
    28681 gccacgttct ttggaacacc ttgatccttg agatatggat gcggtcatgg ctgtgaccaa
    28741 acgcatcgtc gcggttacct ggaccttgcc gccgatcgtg ctgccacgct ccatccaggt
    28801 ttcccgcagc atggccgagc tgacgcggcg cggctgggac gtgcacgtca ttaccgctcc
    28861 gctcgacgca aacaacccgg ccatccccca tgacgaggcg ctcgccgaca tctatcgcgg
    28921 cgcctatgcg cagaccgaga tcgaattccg cgaagggcgg cagcgcagtt caacttggcg
    28981 gcgcttcctg cgctaccatc aggagcaaag aagcttcgac gacgacaact ggatcattcg
    29041 cgccgccagt gcggcgcgcc ggctgctcag gcggaaggac agcgtgttcg tttcgtttgc
    29101 gcagccctgg atcgatcatc tggttgggct caggctgaag cggatacggc cctcaacgcg
    29161 atgggtcgcg catttctccg atccgtgggt cgacagcccc tattacgacg cgaacaatcc
    29221 cgcccagacg gcccgcatga cgctctggcg ccaacaggag cgcgcggtca tcaagcacgc
    29281 cgatgccgtg gtgttcgtga cggcggagac agccgacctc gtcatgcgga agtatccggc
    29341 agcgtggcgc tccaaagttc atgtgacgcc gcatggattc gacaggcggc tgatgccgaa
    29401 ccaaccgctc ggcggcgaag accccaaagc cgggcgccca ctgcgcgtca tctacaccgg
    29461 caacatctat gaaggacgcc gcgagcccgt gccgctcttc acggtcctgc gcgacatgga
    29521 cagggatggg gaattgcgcg gccgggtgca cttcgacttc tacggcaatt cgccggtggc
    29581 gaccgcacgg caagccagcg acatggggct ggacggcatc gtctcgttcc acagctcggt
    29641 cggctatctc gaaagcctcc aactgatcgc gaaggctgac ctgctgctgc ttctggatgc
    29701 gaacgcggac gtcaacgtct tcctgccgag caagatcgtc gactacatga tgacgggcaa
    29761 gccgattctc ggcctgacgc cgcctgtcgg ggcgtccgcg agcgtgctcc ggaagaccgg
    29821 caatctgtac ctcgatccca acgacaaggc caccatgaga acgacgctca aggcgctgat
    29881 cgaggcacac caccgaagcg aacccctgtg ccagttgcgg atggatgcag tagaagcgtt
    29941 tgacattcgc cggaccaccg attccttcga ggcggctttg agcggcgtta tccgaaaagg
    30001 gtgatccggg tgaaaaaggt tcatatcgtc gccggcgcgc gcccgaattt catgaagatc
    30061 gcgccgctgt atcacgcgct ggccggtcac ccctggctga agcccgttgt gatccatacc
    30121 ggccagcatt tctccgccaa catgtcggac gacttcttcg tcgacctcaa acttcccgcg
    30181 ccggactttc acctgggcgc gcgtggagaa acccatgcgc gacagaccgc agcgatcatg
    30241 acggcctatg aggatctggc cctggcgcac cgacccgact gggtcgtggt ggttggcgat
    30301 gtgaactcca cggtcgcggc ggcgctggtg gccaagaagc ttgaactgcc agttgcgcac
    30361 ctggaagcgg gtttgcgcaa tttcgatcga accctgccgg aggaaatcaa ccgcatcgtc
    30421 acggactcga tcagcgacct gttctggacg ccgtcccccg atgccgacga caacctgcgg
    30481 cgtgaaggcg tgcctgcgga gcgcatcgat cgggtcggca acatcatgat cgacagcttc
    30541 ctgatgctgg aaagcgccat cgccgccgtc gacatgcctc gccggatcgg cctttccggg
    30601 cgcccctatg tcgtgtcgac gctgcatcgc ccggcgaatg tcgatcacga ggcgccgctg
    30661 cgcgagatcg tcgatgccta tgtcgcgctg tcggcagagg tggatgtcgt ctttccggtg
    30721 catccgcgaa ccgatgcgcg gctcaaggcc tttgacttgc ggcgcaaact ggagcggccc
    30781 ggtataaaac tgctggaacc gctccgttac atggagttca tgagcctcgt cctgcgggcg
    30841 agcctcgttg tcaccgattc cggcggggtg caggaagaga cgagcttcct tggcattccc
    30901 tgtctgacgc tgcggccgaa tacagaaagg ccgattaccg tgaccgaagg caccaaccgc
    30961 ctgatcggaa cggagtcgct gctttcggcg tccagaacag cgctttcagg gccccgccca
    31021 gagcggccga tcatcgaccg gtgggatggt caggcggccc ggcgcgtggt ggcgagcctg
    31081 gagcgccatg cttcgtccac ccctgccggg aagagctgac atggaccaac ttgcttccct
    31141 ggccgaggat ttcaggtccg agatccgcaa ggtaggcagc atctggatcg tcgcgccggc
    31201 agtggaacat cttgcggagt tgatggcctt cgaaggcgtg gccggcgcca ttctgcaagt
    31261 gcgcgacgat gccctggcag cggcctatcc cggccgcatc ggctggatcg actggacctt
    31321 gcacgaaatc tgcatgccgg atgccagcgc ccagtgcgtg atatccttgt tctcgcggcc
    31381 cgaaatatcg ctgaccgtcc tgaagcttat gcgccaaaga ggcatcagca agattggata
    31441 tttttccatc aagaccgggc gcttccatgc ggatctactg ccaattgctg catttaaacg
    31501 ccttgtgaat gcgatttatt ttcggatttt cgaagaacca cttagagttg ttcgaagtcc
    31561 cgttatcaag aacacggaga gtgcggaaga aaaggactgg agcatcgctc tgggccgcat
    31621 cacgtcgcag acgacaccgt tgcttcccga gaaggccttc aaagcttcca agatcatcat
    31681 tgtcagcgga agtctcgcag ccggcggttc cgaacgccag gtgacctaca cggcgctggg
    31741 cctgcgcaag cgagacctgt cgccgcagat cgtttgctcc gacctggaag gcagcaacgc
    31801 gttttttgtg ccccggctca ccgagcaggg catttccgcg atccaactcc cgccgatgcc
    31861 tggatatttt tccagatcgc gcgccctgct ggaactcaat gcggatattc gctcgatggt
    31921 ggatggcgag gacattttta ccgatgaaat tgcccgctat gccgcgctgt tcctgtcgga
    31981 acgcccaggc gtcgtccatg cttggcttga ctggaccaat gcggctgcgg gattggcggc
    32041 gctggccgtc ggcgtgccga ggatcgtgct gagcggccgt tccttatcgc ccacccattt
    32101 cgcgttcctg cgcagcttca tgcgcccggc gtatcaggaa ctgctgaaga gcgaccgggt
    32161 tgtcctgctg aacaacagtc gggccggcgc cgacgattac gccagttgga tcggcgtcga
    32221 tccttcgcgg atcaaggtca tccccaatgc gatcgatccg gccgagcatc cggtgcctga
    32281 cccggcgtcc gtgcgcgcct tccgggagcg ctacaatctt gccgaaggca cgcctgtgct
    32341 tggcggggtt ttccggctga tgccggagaa gcggccggat ctctggctca agatcgctgc
    32401 cgaggtcgct gaagttcgcc cggacgcgcg cttcatgatc gtaggcggcg gcccggaacg
    32461 cgcgtccatc gaagcctatg cccgtcagct cggtctgggc gagaagctca ttctgaccgg
    32521 cgaggcatcc gatgtggtga cgccgctggc ggtcatgtcc tgcttcctgc tgacatcccg
    32581 cgtcgaagga ctgccgaacg tcttgctcga agcgcaactg agcggcgtgc ctgtcgtgac
    32641 cagcgccgtg ggcggcgcgc cggagaccat cgacagcgat gatgacacca gcctggccat
    32701 cgatggcgat gatcctgcgc cctacgtcag ggccgtgctt gccatcctcg gcaatccgga
    32761 acgtctgcgc cagtcgaaaa caacgcttcc ggcgatcatc ggcaggaagt tcggatttga
    32821 cgcgatgatc gacaggacgc ttggcatcta tttcgacaag tagcggccga cacccagtcg
    32881 ggcagcgcgc gggtcacgga aggcgttcac gcggaatacg cgtgacatgt cccgccttca
    32941 gtaggcttgc tcaaatctcc gacatgggca agaaggtcaa gcatccagtg acaacgacgt
    33001 gagttcccaa gatgctccag tcggaagccg cggctgtcag ttcggcaagc gcgcataaag
    33061 ccgaagcgag accggccgtc tcggtcgtct gtccgttctt caacgaagag gcgatcatcg
    33121 agcgcgcagc ccagcgcatg ctcgacaacc tcacgacaca gttcggcgac gaattcgaac
    33181 tgatcctcgt caatgacggc tcgcgcgatc attcacttga acggatcacg gcacttctgg
    33241 ccacccatcc gcggtctgcc caggtccgtg tgctgaccta cgcattcaac caggggcgag
    33301 gtcgggcgct gaagacgggc atcgacgccg ccagggcgga gatcattgtc acgacggaag
    33361 ttgattgctc ctggggcgac gacatcgcgc agcggctgca cgctgaactg gtcgcgaatc
    33421 cgcaggccga tttcgttatc gcatcgccgc acagggaagg cggcagcctg gtgaatgtgt
    33481 ccgcgtcgcg cgcctttctg accaaggccg ggaactggct gatccgccta ttcttcgaca
    33541 gccgtgtcac gatgaacacg ggcatgacgc gtggctatcg cgcgcgtgtc atccagccgc
    33601 tcatgaccga ggagaacggc aaggagtttc acctggaagt gctgctgaaa ctgctcggac
    33661 tgggttttcg ggtgcgcgag attccggcca ccctcacctg gctcgatgaa aagctgcagc
    33721 gcgccaattc cgccaagcgg aagtcgtcga cgcggatcct gaagaccatc aacagccatc
    33781 tgcggttcat cgcgatcgcg cagccggtac ggcattatgc tgcgctgaca gccctgaccg
    33841 ttctggccgg cgtggctttc ctgatggccg ccgtagccat gctgatcacc aaagacgccc
    33901 ctgccgtgta tcttgcgatc gtgggcctaa acctgcttgt gatcgcgctg cttttcggag
    33961 ggttctccat tctcttcttc cagttgcggg aactttcgcg tctgaactgg atgaagggct
    34021 atgaaagccg gacgccaccg accgcccttc cggtgcacac cgcctttcct gcgcaaagat
    34081 gaagacctga catgtgtgga atcgtcggaa tcgcgggcag gcatgatcct gaccttgtcc
    34141 gccgcatggc cgatgccatc gttcatcgcg gtccggacgg agcaggattc cacaattgcg
    34201 ccagcgccgg catctgcgtc ggcatgcgtc ggctcgcgat catcgatctg gtgacaggcg
    34261 accaacccct gcacagcgcc gaccgcaagg ttcacatcgt atacaatggc gaaatctaca
    34321 atcatagaga agtacgactg gagctggaaa agctcgggca tggcttcaag accagctccg
    34381 ataccgaagt tgtccttgaa tcctatctgg cgtggggcgg agaagcctgg cagcgcctgc
    34441 acggcatgtt cgccctggcg atcatcgatg agcgcgggca caggccgaag ctgatcctgg
    34501 tgcgcgaccg tccgggcatc aaacctctct acctcgcgcg ccaaggcaac actctgcttt
    34561 tcgcctcgga ggtgaaagct ctgcaagcgg acgccgggct cggttccgaa gtcgatctta
    34621 acgccgtgcg cgcctatttg tcgctgcgct ataccccggg gcccggcacg ctgttgtcgg
    34681 gcgtggaaaa actgccgcca ggccacgaac tgacgtggtg cgacggggac gtcagggtgc
    34741 ggcgctggtg gacgccccct gcccccaccg tggtcgagcc tggactttcc ctggatggag
    34801 ccgccgaccg actcggcgcc gcgttgcgga catccgttca ccgccacatg atcgcggatg
    34861 tgccggttgg ggcgtttctt tccggcggca tcgattccaa catcatcgtt gccctgatgg
    34921 cggaggccgc cagcaagccg gtcaagacct tttcgatcgg ctttcccgat tttccagacg
    34981 acgaccgcaa tctcgcggcg ctgaccgccc gccgcctggg gacagaccat catcccatcg
    35041 actgccgcgc cgaagacatg gcgatgttgc ccgacatcgc cttcaaactg gacgagcctg
    35101 tcggtgacgc gatcgtcgtt cccatgagtg tccttgccag ggaagcacgg caacacgtca
    35161 aagtcgtgct ttccggcgaa ggggccgacg agatcctcgg cggctacatg ttccatcgca
    35221 agctcaagca gatcgcgaag gtgaagtccg tcgtgccatc cgcggtgttc ggcctggcgg
    35281 ccgacgtggc cagcgtcatg ccggtcgctg tgctggagcg cctgtttgac tatcccggag
    35341 cgctcggcga agaggggaag cgcaagatcg tcgagatgac ccgcaggatc ggcagcgcgt
    35401 cgctgtcgtc cctctaccga tccgccgttt ccttgttcga ccctgacgat atccgcgccc
    35461 tggcaggcca tcctgccctc gcccggctcg cggacgtggc gcctggcccg acacttgccg
    35521 aaacgccggg ggcgaccgca ctgcaggcgc ttgtggacat gcagtatccg gactggctgc
    35581 ccgacgacat cctgatgaaa gccgacaaga tgtcgatggc ccattcgtta gagggccgcg
    35641 tgccgttcat ggacgaaatc gtgatttccg cggcagcctc cttgcccgat cacacgaaga
    35701 tcggacgcgc cggcaacaag ctggcgttgc gacggttcgc ctcgcggctg ttgcccgaag
    35761 aggtggtgcg ggcgcccaag cgcgccttct acatcccgct ggaatcctac gcgcgcacgc
    35821 agccgctcgc cgggattttc gactgggcgc tcgacagcgc ccgcgtcaag cgacgcggac
    35881 tgttttcgcc ggagtggatc gccgcgcagc gcaatgccgg cccggaaaag ggatttctcc
    35941 cgctcaaacg tctgttctcg atcgtcatgc tcgaattgtg gttcgaacgc tttgcaccga
    36001 acgtgggctg gacctgagcc tcgacctgac gcagaagggc aaccgtgaaa atcgtcgttc
    36061 agaacttcaa gaccggtgtg ctgtcggtct ccgacagtcc cgcacccagg gccggcgccg
    36121 gtcaggtgct ggtgcgcgcc cacgcctcgc tgatcagcgc cggcacggat cgggccatca
    36181 tcggcctggg caagaaaagc tacctcggga aggccctcga tcgacctgac ctggcgcgca
    36241 aggtcatcaa ccgcgcccgg accgaaggcc tctggccgac gtacaaggtc gtcaagaacc
    36301 tgatcgcgga accaataccg ctgggctatt cactggtcgg cacggccatc gaagtcgggc
    36361 gcgacgtcgg cgacatatcg gtcggggacc gtgtcgcctg cgccgggctt ggctatgcca
    36421 accatgcgga agtggttgcg gtgccgaaga atctctgcgt gcgcgtcccg gatggcgtga
    36481 gcgacgagca ggcgtcctat gtgacacttg gcgcgatcgc catgcatgga ttgcgtcagg
    36541 ccgaccagca actcggcgcg agcgtgctcg tggtcgggct tggactcgtg ggccaattga
    36601 ccgtccagct ttgtgtcgct gccggactgc gtgtcgccgg tctcgatctc gatcccggca
    36661 agctcacgct ggcaaggcag gcgggcgcga gtctcgcgat gacgccgtcc gatccttcac
    36721 ttggcgcggc gattgccgag cttaccggcg gatatggcat tgatgccgtg ctgctgacgg
    36781 ttggctcgcg cgatagcgga gctgtcttcg aggacgtcgc caggctctgt cgcgaccggg
    36841 gccgcgtggt ggttgtcggc gatgtgaaga tggacatctc gcgccggacc tatttcgaga
    36901 aagagctgga aatcctgcaa tcccgttcct acggccccgg acgctatgat acggcctacg
    36961 aagcggcagg ccacgactat cccatcggct atgtgcgctg gaccgagcgc cggaatctcc
    37021 gcagttttct cgacctggtc gccgacaagc ggatcgatcc ggcaacgttg accacacatc
    37081 ggttttcgat cgatgaggcg gcgcagggct atgatctcgt taccggcaag accaaggctt
    37141 tcagtgtcgg catcctgatt cagtatccgc ctcgcgaaat cgagcaagcg gtctcggcgc
    37201 gaccggccgc cggtgccgtc agcggcaaga tcgggcttgg tgtgatcggc gcgggcacct
    37261 tcgccaaggg catactgctg cccgctctca tggacaccaa cgcctttcgg atgcacggcg
    37321 tcgcgagcgg tcgcggaata tcggcgaaga gcgtggcgga tcgctatgga gcaggcatag
    37381 ccacgaacga cgcccgccaa ctgctcgatg acgattccat ccaggccgtc gtcatcgcca
    37441 cgcgtcatga cagccatgcc cgctatgtga tcgaggcgct caagcgcggc aagcatgtct
    37501 tcgtggagaa gccgctggcg atcacggcgg aagaactcga tgcggtggag gccgcagccc
    37561 gttccacgtc ggccaccctt acggtagggt tcaaccgccg gttcgcgccg ctggtgcagc
    37621 agatggcggc gcatttcgcc ggccgtgtcg agccgatggc catgctgtac cggatcaatg
    37681 caggccgcat cccgctcaag tcggaactcg cctgggtcca tgatcgtgcg accggcggcg
    37741 gacgcatcgt cggcgaggcg tgccacttcg ttgacacgat gcagtttctc tgcggcgcgc
    37801 tgccggtgtc ggtccaggtc acctctgcca acccgcgccg ggccgatgtc gctcacgatg
    37861 acatcgtgac gttcacggtc agcttcgacg acggctcgat cggcagcgtg cactactggt
    37921 ccaatggtga cgccagctat ccgaaggagc ggttcgaggc cttcgcgcag gagcggatgg
    37981 ccttgctcga caacttccgc gcgctggatc tggtcgctgg caacaagacg acgcgcaagc
    38041 gctccttcaa tgtcgacaag ggttttgcgg cggaggccgc cgcgttcgcc cgcgcctgcc
    38101 ggggcggcga tcctggaatt gcactcgaca gcctgctggc aacgacccgg gtaacgctgg
    38161 cggtagccgg cgcgcttgga ggcgcaggtg cacacgtcga cgatccgatg gccgaagccc
    38221 atgactgaag ccgggtcgcc atgcgtcgtc tgcggatccg tcgcccagcg ccgcgcccgc
    38281 gtccgtctgc gctacggcac catcttcgag tgcgctggtt gcggcaccgg gttgctcgaa
    38341 ccacgtccca cggcagccgg cctcagcgcc ttgcacagcg aggaggcgta tttcagccat
    38401 ccctatttcg aggaccggcg cgaactcacg gccgcgatgc gcgcctccag tgaggccaaa
    38461 ctctcgcgca ttgaagcgcg cgtgggttcc ctcgccggga aaacgctggt cgatgtcggc
    38521 tgcgatcttg gcgtgctggg cgcctatgca accgaaagcc gtgggatgac cgccatcggc
    38581 atcgatatca tcgccaaggt ggtcgagatc ggacgcgctg cgggccgcga cctgcgccac
    38641 ggcacgctgg aaagcgtcgc cttgccgtcc ggcagcgccg acctcatctg tggattcgac
    38701 ctgatcgaac acgtggacga cccccgcggc ctcgcgcgcg aggcgtggcg cgtactcaag
    38761 cccggcgggg tcctggcgct ggaaacgccc aactacaacg gcctggtcta ccggatcggc
    38821 cgatggctgg gtggagcgcc gggccttgcg tcggtcatcg cggggttgca ggagcggctc
    38881 tggcccccgt tccatgtcca gtatttcacc gccgattccc tccgcaatgt tctggatggc
    38941 gcggggtttg ccgatgtgaa ggttcagggg cgcgaactgg acacatccga gctggcggtt
    39001 tcaagtccgg tgttgcgggc ggccgtgctg agcgtgtttg ccgccgcgcg ggtcaccggt
    39061 tcggccacgc tgcttacggc gatagcccgc aagccgcggc aaaggtgagt gcaatgatca
    39121 ggctgctcgc atcgggcgtg gtgggcggct gcattgtcgg tggccagatg tgcctggcgg
    39181 cattcgcgcg cgcgctggca ccacccttca cgattgcgaa cctggcgtcg caggcatttg
    39241 cgatgccttg gctctatctg gctggcgcgg cgtatagcgt ggcggtgctg ctgtatctga
    39301 tcctgttgcg cacaggcgcg atatccgcca ccaacctgcc catcatggct gtgattgtcg
    39361 tgctgaacat cgccatcgcc ctgtctcccg gcgatgcgct gacggcgcgt cagatcacgg
    39421 gggcggtcct tgtgggggcc ggtaccctgc tgatgttgaa ctgaggcacc gccatggccc
    39481 atgccgatcc ctatgttcat gccataacgc cacttctcgg gcgcgcgctg tcggggctcg
    39541 acgccgagat cacgtccccg tcacgcggga gtttcgaccg cacctggtgg tgctggaagt
    39601 tcacggattt ttccgcgacg cgatttcagg aaggcgcgca tgtgcttgcc tggctggcga
    39661 catcgccggt gtcacctgcc ccggaagcgt cacgaccgcg ctttgctcag ttatgcggcg
    39721 catccttggc cttctgggct cgactgcagc atggcgacgg cagcttcgat gaagcctacc
    39781 ctttcgaacg cagccttgcc gccaccgctt tttccgcctt ctatctcggg cgcgcaatcg
    39841 agcggctgcg cggcgccctg gcgccggaag tcgagtctct tgcgcttcga accctggagc
    39901 ggagcgcggg ctggcttgct gccaacggcg agtatcacgg cattctctca aaccacctgg
    39961 ccgctgcggc tggcgccctg caaatcgccg gcgacgtcct gcaaacggat cgtttccgct
    40021 cggggcgcga tcgctacctg gggctgatct accgccatca gcatccgtcg gaaggctgga
    40081 tgcgcgagta cggcggcgcc gatccgggat accagagcca cggcatgttc tacctcgccg
    40141 agatcgagcg gcgaacgcga gacggggaac tgttcgagcg cttgtcgcgc ggctgtgatt
    40201 tcctgcgctg gttcgtgcac ccggatggga ccattggcgg cgaatacgcc agccgcggca
    40261 ccaagttttc ctatcccgcc gctttcgagt tgctggcggc gcgtcttcct gccgcggctt
    40321 cgattgctgc acacctgcgc gagcagctgc gggacggccg tggtgtcggc gcacgggaga
    40381 tggatgcctg gaaccagttt ccgctcctca acaatctgct gtttgcggcc gacgccgccg
    40441 gtgatctggg ccggccggca cctctcccct ggcatcagga tggcagcacc gcgacgtttc
    40501 ccgatgccgg ccttgtcatt gcccggcgcg ggaggcgcat catcgcctgc ggcgccaaca
    40561 tgggcggcgc ggtgaagata tggtcggccg acggcgctct ggagtacgag gattgcggct
    40621 atgcggccca aagcgctgat cgctggttcg tcagccaggg cgagggcagc gcgcatatcg
    40681 acgcggtggc tgaaacggcg ctgaccgtga ccgcgaagac gccgtttcgg ggcattccga
    40741 ccactcggtt cgatccctgg cgcttcatcg ccttccggct cttcacgacg accatcgggc
    40801 ggctgccttc ggtagcgcga tggctcaagg agctgctcgt gaaagtgctg atccgcaaga
    40861 aacagcctca tcccgggtcg cttctgcgcc atgtcacgat cgcatcggac ggttcagtca
    40921 cgatcgatga caccatcagc gacgtggcga ccgccccggt cccgcttccg cgccatgttc
    40981 cgtttcacat ggggtcgtcg cgctacgcgg acttcgcgga ctggtgcgga gccgaaaggc
    41041 cctgccctgc tcccaccgcg tcggagggcg gctaccgccg cagactcgtc ctggatgcgt
    41101 gacgcgacgg tcagcccggt ttgcgggccg aaaaatccat gacgccgcca cgcccggcaa
    41161 gccaggcgac cgtctcaaac ggcagcacgg ccaggatgat ccaggaatag acaggcgtct
    41221 tgccgaagcg catcgacggc ttccagccct tcgaaatcag cgtgttctgc accgacagcg
    41281 ccgcctgaac atggggcgcg cttcgaacct tgacgtcaac gaacccggcc tctgtcaggc
    41341 accgcgccag gccgccgtat gaaaacgcct ggaggtgacg ggggaaatga taaccgcccc
    41401 agcagcggcc cgcgaggctt tcctcccagc aatcgcgcgc aggcaactgg cccagcgccc
    41461 tgccgccggg tttcaggatt gcaaaggcgc ggcgcgccat ttcccgcggg tcaaggacat
    41521 gctcgatcac atggttcatc gacacgatat cgtaccgccc gaggtgacgt gcgaggtcca
    41581 tttcctcaag cgtgccggtc tccacgtcgt acccgcgcgc gcgggctttt tcggcgattt
    41641 ccggcttgat ctcgacgccg ctgaattcaa gcgagcaata gggcttcagc gcatcgaaat
    41701 gccggcagtc gcccgctcca acgtcaaata ggcgcccgcc ttcagaaccg atcatcccgc
    41761 gatatgagga tgccctggcc cgcgaacgca acgacaccag gagccgggct acaccggagt
    41821 gatcgtcgtg ataggcgtgg tagtcgtcgg ggtaaaagct ggcgatctcc gccaccgtcg
    41881 ggcgtggagt gaggaagtcc gactggcagt catcacagcg ggcaatcctg aactcacgat
    41941 ccgggcgcac gccgtattcg aaatcgacga catcgcttct gtgcaggtgg acacgaccgg
    42001 aaccgcaaac gggacaggat tcgacttgat caggcatgca ccgtgtcctt ggaggaagaa
    42061 actgcttcag gatggatcag ccgacccagc acctgatagt agctccttcc ataggcatgc
    42121 ggattggcct tgtaaaatcc ccgatcgtgc aggagttgcg acaacgccgg cagcgacgtg
    42181 tagggcacgg ccggccagac gtgatgttcg aagtggtagt tgaatttcac gggcgcgagc
    42241 aggaatgccg tcagccagtc cgtggagatg gtgcgcgtga tcgcgggatc gagttgctga
    42301 tcaaactgct cgatgccggc cagggcgtac tcatcgctct ggtgttccgc gatcacccgg
    42361 atcgtcgcta gcagggaggt cagcgtcaac aggggcagga accaaagata cagccacgcg
    42421 aaaggagcgc cccaatacca gcaccaggcc aggatcacac cgttggttcc agccgtcaga
    42481 accaggaaac gaagcctttg cctcccgtcc tccgggtcac ccagggactg gctggcgtat
    42541 gtctttgccg tcagaagcgc cgaagagccc aagagggtga ggatgcatct tttccaaaat
    42601 ctcagaccct tgataataaa tctcgatttt atttctgtat ctttttccgg atcaccaaga
    42661 tccgtgtgat gaagcttatg ctttgatcga tagtcacgcg tggcaataaa atgcggcgtc
    42721 gctgcgaaaa tatcgcagat ccagtcattc agagccttgt ttcgggtcag cgaaaaatga
    42781 gatgcctcgt ggacccaatt gttcagcgca tgctgacgtg ctgaaataac aacgaatgac
    42841 aatacaaaaa caagcggatg gcgggtccat ccccacagtg ctagcgcccc aaggatcagg
    42901 aaccagtcga acgccaggtc cctcgcgaca atccaggggc gcggcttgtg gaacgcacgc
    42961 aactccgcgt ccgtgatgta acgccgggga tcattcaggg cgcgggactc tgcagcctgt
    43021 ttggtcattg ttcgggcctg aaatgtgggt tgccttgcag ccgacagccg tgctcacgct
    43081 gaagcagcgc catcagccgg tcgcgagttt gtagacgaca aacctggagt tctcaaacgc
    43141 cctttccaga ggcaactgcg gcaatttgac aggaccacag gtccagcctt ccggctcggt
    43201 cccgtccttt ggcgcgacga tcagccgaag tgcgggatag tggcgccgca gcgcttgcag
    43261 cctcccggcg tcggagacaa ggtgcacgta accgcgttcg atcatgcaga gcaatccgcc
    43321 ctgttcggcc agccaggcgg cgaccgaatg cgccggacgg tcgcgcaggt cgatgccata
    43381 gagaaccatc aactggttga attcgaaagg caaaagccag cgtgcataga ccgagaaggc
    43441 gagctggcca tagtccgtcg ggctcgcctt gcgggacgac atcatggcgt agggataagg
    43501 cggcagcagc gccatttcag ccggttcggt tctctcccgc aggaaacgcg tgacttccga
    43561 ccattccggc ccatcgggcc tgggcatgga agccagggtc tcgcgcgcct tcgcggggac
    43621 aagccacagc ggaacggcga gcgacaccgc gcaggccacc atcgccacga cgcggcccag
    43681 cgaaaccgcg gctccgggag ttctcggcgg accaagcgtc ctggcggcaa gccccgcgag
    43741 cgccagcagc acaagcgcaa gcgatcgcag ttcgggcccc gcttcgggca ggctgatcaa
    43801 cgcgagggaa agccctgcga gggccgcgga gccaagactt gccaaatttc cggattggga
    43861 ccaggcgcgc acgttcaaga ccaacagggc ggccaggagg gcgggcagcg tcgcgacaca
    43921 gatcagggta gcgcgagtca gcacaagtcc cgcaatcgtt ggcgtcggcc tccaggcaaa
    43981 ggccacatag gccatggccg aaaacacaag ggccgcgaga ccagtcgcca ccagccgcag
    44041 tctccgttcg gatgcgacga ggggccagat cgcgaagacg gcaaggctcc aaagcgcagc
    44101 gagccaggcg agcggctgga cgacgccgtt ttctccccag atgaagatat gcgacggctt
    44161 gcgcgccgcc atgagtgtcc accacgccgc cgggtcgggt atcggcccct gcgccaccgg
    44221 cgacgccagc gcatgcagca tgagcatcac catctgcggc gatgcgcaaa cgagaaacgc
    44281 ggccaatcct gcgacacacg aacggatcgc gacggcgaag gcccgctggc tgagcgcctc
    44341 caggccaagc ccggtcagca ttgtgcccag caggatcacg ccgaaggtgc tgtgaaggtc
    44401 gaagagaagg ccggcgagcc cgaaggcgat cacgtttgcc cgcattgatc gcgctgagaa
    44461 cagccaccat gaccagatcg ccagcgcggc ggcgagcgcc caggcgccca gcaggtaccc
    44521 gggcatgtgc atcaggagcc catagccaag atgcatcttc cctggccagg cctgcacgag
    44581 tgccgctatc gcgaggcatc ccgcgaggag cccgacccaa cccgatgtcc tggaaagctt
    44641 tgcggcggtc atcgcgactg caccgagacc cagcatcgca aagaccgccg cgagcccatc
    44701 gtgaagcgcc tgcacgggca acccgaacgc cttctgaagc atcgccgcaa gacggggcaa
    44761 cagcacctga agatgcgggt agacttcggc ctggatggtc ccgggatgcg ccaccaactc
    44821 accggtcagc cacagcgccg gcgcgatcgt gtcgctgtaa ttcggattga gcccggccag
    44881 acgccagagc agcacagtcc aggcgccgac gacaaggatg atcgcccaga cggggctggg
    44941 ggcggtggcc cgcaaagcat ccctcgcctt gctttgaagg tgctcgacaa tccggtcgga
    45001 ctgcctgcgg accgaatgcc atggcaagaa cagcgcagcc gtcccaaggg ccatggccgc
    45061 gatcgcgccc ggctgcggaa ggaggcgcga gcccagcgcg aaccagtgcg tcaggagcag
    45121 cccgatgaac accccgacgc ccgggcc
//
LOCUS       DAOMXN010000007        43365 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_623_length_43365_cov_12.259686, whole genome shotgun sequence.
ACCESSION   DAOMXN010000007 DAOMXN010000000
VERSION     DAOMXN010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 43365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43365
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_623_length_43365_cov_12.259686"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="PK812_01560"
     CDS             <1..792
                     /locus_tag="PK812_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HRJ68268.1"
                     /translation="TRTLGPLLPVHRVALANIAAAFPEKLEAERRAIARGAWENLGRV
                     AAEYAHLHHLFDWDADQPDKPSRCAVEGIEHFIALRDDNRPGIIFSAHLANWELPAIC
                     AATYGLNVTAVFRAPNNPAVNRVVHAVRSGTMGGLEAARQGAAFAMKDALEKGGHLGM
                     LIDQHFTRGVAVPFFGRPAMTNPIMAKFAEAFDCPVHGARVIRLPGYRFKLELTPPLE
                     LPRHPDGSLDVPGAMAAMTAVVEGWVREHPEQWLWMHRRWRTPTK"
     gene            870..1910
                     /locus_tag="PK812_01565"
     CDS             870..1910
                     /locus_tag="PK812_01565"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HRJ68269.1"
                     /translation="MQQTPDPVPEGRAAAEAAPAPVSETAAFRRRTAMQRLGALASAA
                     LFIMALVVLYLVISELDFDEVKAVFGRTSREQLLMALGLTVLSYLMLTGYDALALWQL
                     AKGQVRYRLTALASFTSFAVSFTLGFPLITAATVRYWVYSAVGLGPGVIASLTLIAGL
                     TFWLGMSVVLGLVMIAQPFATSVFTRLPAQVNGYVGFAILSAIFLYTVWIAARPRHIR
                     IQGWTLHLPRLRVSLLQMLLGAVDVCAACGVLYVLLPAGHGVPFAAMVAAFVFACVLG
                     IASHAPGGIGVFEATLLLALPGIPKEALLGSILMYRLCYYVLPFIAAIWLLGLREIWL
                     RARRLRSEISGS"
     gene            1915..3129
                     /locus_tag="PK812_01570"
     CDS             1915..3129
                     /locus_tag="PK812_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRJ68270.1"
                     /translation="MAGRWVLAGAMAIAAILFGVAIDGWSGLLIGTVALGAGGVAAKL
                     LSGRGEQSGASAGHQSAQPGNAGMQEALETAPLAALLLARDTTILFANREARRLFPSL
                     RTGDPLSLRLRVPEVLDAVAATLRDHVAQGTDFSERRPVERSFHARTFPLTAESGKAP
                     ALAVFLEDASGLRRVEAMRSDFVANASHELRTPLASILGFIETLQGPARRDAEAQQKF
                     LAIMADQARRMARLVDDLLSLSRIQLKEHIPPTARVDVRGVVEQIADALSGLARERGV
                     TVAIMASGPAVVAGDRDDLLRVFDNLIENAIKYGRSGGKVDVSIEDEAATPDIVIRVR
                     DYGPGIDPQHLPRLTERFYRVDTTVSRAEGGTGLGLALVKHIVNRHRGRLDIDSEPGM
                     GATFSVSLPRPK"
     gene            3209..3910
                     /gene="phoB"
                     /locus_tag="PK812_01575"
     CDS             3209..3910
                     /gene="phoB"
                     /locus_tag="PK812_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019998202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HRJ68271.1"
                     /translation="MAALIWIVEDEEALSLLLRYNLEAEGFAVEVITRGDDADVRLKE
                     QQPDLLLLDWMLPGVSGIEICRRLRSRDGGERLPIIMLTARGEEAERIRGLKTGADDY
                     MVKPFSIPELMARVRALLRRAAPERVTTMLRIADLEVNRETRRVRRGGKELHLGPTEY
                     RLLEFLMSRPGRVFSREQLLDGVWGSETYIDDRTVDVHVGRLRRALNTTDQRDPIRTV
                     RGAGYSFDETFGAAG"
     gene            complement(3913..4410)
                     /locus_tag="PK812_01580"
     CDS             complement(3913..4410)
                     /locus_tag="PK812_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcrA family cell cycle regulator"
                     /protein_id="HRJ68272.1"
                     /translation="MSWTDERIERLKKLWSEGLSASQIAAELGGVTRNAVIGKVHRLH
                     LSGRVKTSSTTAQRSRKTVRSQVRATPRVTTIARGNLAVVQLLDTEAVVAYRPAEEVV
                     VPISRRISIMELREGTCRWPMGDPLQPDFVFCGGDCEVGKPYCMAHARVAFQPSQDRR
                     RLAPR"
     gene            4763..5980
                     /locus_tag="PK812_01585"
     CDS             4763..5980
                     /locus_tag="PK812_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRJ68273.1"
                     /translation="MTKAGGEKKTESAVLPTYARADLRFERGEGSWLITDTGDRYLDC
                     VGGVAVNALGHCHPHLVETLQKQAARLWHTSNLFRIPEGERLARRLADLTFADQMFFT
                     NSGAEALECAIKMARKYHAVTGHPERYRIITFEGAFHGRTLATIAAGGNKKYLEGFGP
                     EMPGFDQVPLNDIDAVKTRIGAETAGILVEPVQGEGGVRSATPAFLRELRQICDENGL
                     LLIFDEVQCGMGRTGKLFAHEWPGIAPDIMAIAKAIGGGFPLGACLCTAEAGKGMTAG
                     THGSTYGGNPMAMAVGNAVLDVIAQDEFLAEVQRKALLFKQRLAELKDRYPRIIAEIR
                     GEGLLLGLKAVVPNTDLVAAAREQHLLAAAAGENVMRLMPPLNITDQDIAEVLKRLDA
                     ACAALEGKARSAA"
     gene            6003..6932
                     /gene="argF"
                     /locus_tag="PK812_01590"
     CDS             6003..6932
                     /gene="argF"
                     /locus_tag="PK812_01590"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HRJ68274.1"
                     /translation="MTALRHFLDLSALRPDEFTGILSAAARMKKRRGTPAADQDRPLA
                     GKKIAMVFEQPSLRTRVSFDVGIRELGGEPLMITGKEIELGERETIADTARVMSRYVD
                     AIMIRMLDHAQLYELAAHATVPVINGLTKRSHPCQVTADIMTFEERVGPLAGRTVAWT
                     GDANNVLASWVQASALIPFTFRIATPEELAPRPELIAEARKAGGRIEVGHDPIKAVEG
                     ADCVITDCWVSMGDKDEGRRHNLLRPFQVNRRLMSAANQGAIFMHCLPATRGEEVTDE
                     VMDGPQSAVFDEAENRLHAQKAVLAWVFGAAVS"
     gene            6942..7928
                     /locus_tag="PK812_01595"
     CDS             6942..7928
                     /locus_tag="PK812_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone"
                     /protein_id="HRJ68275.1"
                     /translation="MNEPLTRFVSDAPGAGDDAVLPFQVDALDARGRLVRLGPVVDTI
                     LHRHAYPAQVARLLGEAIALTALLGASLKFDGKFQLQTRTDGPVGMLVVDFTAPSSLR
                     AYARFEADRIVAGAGTASLLGNGHLALTIDQGPHMQRYQGVVPLDGQGLETAAHQYFL
                     RSEQIPTKVRLAVAEETTPTDSGPRARWRAGGILIQFLPKSADRQRQAELDPGDAPPG
                     TAAAPFAEDDAWVEARSHVETVEDHELVDPAISGERLLYRLFHERGVRVFERNPLVER
                     CRCSSQRILDMLKSFSADDRQAMVGDDGRIGVTCEFCSVHYGFDPADAESAS"
     gene            complement(7921..8313)
                     /gene="apaG"
                     /locus_tag="PK812_01600"
     CDS             complement(7921..8313)
                     /gene="apaG"
                     /locus_tag="PK812_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009339621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HRJ68276.1"
                     /translation="MYRAVTDDIEVVVRPQFSQDRSQPEKNQYFWTYDVEITNHGDET
                     VQLRHRHWEITDGNGQRQDVRGPGVVGEQPILKPGDTFRYASGCPLSTPQGIMVGEYE
                     MVDAQGRRFQVQIPAFSLDSPFEKRQLN"
     gene            8415..9047
                     /locus_tag="PK812_01605"
     CDS             8415..9047
                     /locus_tag="PK812_01605"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRJ68277.1"
                     /translation="MLDGVMRRIVTPPLDGIAAWCAARGIAANAVTLLGAAFGLACGA
                     CIAVGWFMPALACLAASRIADGLDGAIARRSGITDFGGYLDIVCDFVFYSSVPVGFAI
                     LDSGRCAVAASLLLASFYLNGASFLGFSALAAKRGMDTDVRGRKSLYFTTGLMEGSET
                     IAFFAAFLIWPEYFPVLAPIFTALCLVTMLARMALAWRVFSVEDSGRRQG"
     gene            9101..10189
                     /locus_tag="PK812_01610"
     CDS             9101..10189
                     /locus_tag="PK812_01610"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018044102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HRJ68278.1"
                     /translation="MTGSSGIHSDTMILGLIQSGTIQLAAPVATGQVQPASLDLRLGA
                     RCWRMRASFLPGPQRQVRERVDALALHEIDLTKGAVLETGCVYIAELMESLALPKGVS
                     ASANPKSSTGRLDVFTRVITDRGAAFDVVPEGYAGPLYAEISPKTFPVLVRTGSRLSQ
                     IRFRSGTPRLDDAELSALNAREALVSGGRHPIAGGVAVSVDLSGFDAGGLIGYRGKRH
                     TGLVDVDRVGGCRILDYWEPLTADGTGALVLDPSEFYILASKEAVHVPPDYAAEMVPF
                     DPLVGEFRVHYAGFFDPGFGHAGAGGAGSRAVLEVRSHDVPFIIEDGQIVGRLVYEHM
                     AERPATLYGAGLSSNYQAQGLKLSKHFR"
     gene            10233..10307
                     /locus_tag="PK812_01615"
     tRNA            10233..10307
                     /locus_tag="PK812_01615"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10265..10267,aa:Gly,seq:ccc)
     gene            10613..11548
                     /locus_tag="PK812_01620"
     CDS             10613..11548
                     /locus_tag="PK812_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRJ68279.1"
                     /translation="MLTLRQIEVIRAIMVTGTVGGAARLLHVSAPGVSRTMKHAESVV
                     GIKLFSRKGGRYTPTREASDIFGQINAVYDKVEDLQFVIDRMKKGEEAELRIGSVPSL
                     SNVMVPRAIADIRSAYPSLRIDFDVLKIEDAIDYLLLGKGEVVAMSYRIEHPMLSFQP
                     LATGRLKCIVPAGHPFGGRKRISAQEIADHPLIGIDPNDPYGRIMAGIFAASAAKYDV
                     TIRARFGSTVCALVTNGLGIAVIDEFTLAGGNWPGLRALDIEEPTAFQTYIAMRRDAA
                     LTGYCEAFVTRLRHHMQALSPGKPALGGVGRSGKN"
     gene            11691..12698
                     /locus_tag="PK812_01625"
     CDS             11691..12698
                     /locus_tag="PK812_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014130376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRJ68280.1"
                     /translation="MMRHMFKLAALAAALAAFAAPASAQPYPNQDIQGVIQWGAGGST
                     DVVVRAVTPHAEKVLGRSVIMTNRTGGVGAIAMKFVQSQKSDGYTLLMGAENPQLYKV
                     MGLSDTDYTDSFTTINLLARGIPILVANKDAPFNTAKELEAYAKANPGKVKVGSTGPG
                     GLPSVVMAMLGTQVKLDLTAVPYDGDGPALTALQGGAIDLMPAVLGAAIEHIKAGRMK
                     PIAVIDTAANPALPNVKPIVADYPGFSKLLPWGPFFGIFVKKDTPKDVVDKLVQAFNQ
                     GAKHPDFVKLIDDRGFKMMNIAGAEADDFLKRWQANTSWIVFDAGIAKKSPAEFNIPK
                     P"
     gene            12770..13297
                     /locus_tag="PK812_01630"
     CDS             12770..13297
                     /locus_tag="PK812_01630"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HRJ68281.1"
                     /translation="MDPQPTRRPGETVFTVMMLLLSIFLLWNAYKISGFSSKSSPGAF
                     PLAATLVMVVAGLVAVFNTLRLPEGPKGFSAFRSQVAPNLVLAFAGLILFYGLALESV
                     GFILTSLAFLFAGMLLLHRQGIGRSLMYSVLSLALVYVTFRLVFKVVLPEGIIPERRI
                     LAEIGAVLGPYLGVR"
     gene            13297..14808
                     /locus_tag="PK812_01635"
     CDS             13297..14808
                     /locus_tag="PK812_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014130378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRJ68282.1"
                     /translation="MEALKYFTMAWFDPNLLFLTAIGTFAGIYIGAIPGLSVTMAVSI
                     LIGFTFSWEVNSALALIAGIFTGGVYGGSRTAILLNIPGAPAAIATGFDGYPLAKKGE
                     AGNAIGISTVMSVIGGFFGILVLAVGAPIISEFAVRVQPRDYLLLALMGVLLVGALSG
                     ESLAKGIWAAAFGMVLGSVGLDPLTVEERFTFGLPQLTNGISAIAVMIGMFGLSEALI
                     LLHDLDKKAIKQRVDNIVPKWSDIKKYFGLSMQSSAIGVTVGALPGTGGDIAALLAYD
                     HAKRVTKNPEVPFGQGAKEGIVAPEAANNAAVGGSYIPMLTLGIPGDAVTAVIIGALF
                     IKGLNPGPLMLIEKPHMFWFTVGNLTLANCFLLIFGLTGIKIFAKIVEIPKGVLLPLI
                     VLLSVVGAYSINNSADDVLWMIAFGVFGYFLKLYGYQTGPLILGIILARLIDDNWRRA
                     IISERGDMTAFFLNLVSSPLSAILLGIIVAVLLSKTPLWPKRAQTKARAEGST"
     gene            14805..15689
                     /locus_tag="PK812_01640"
     CDS             14805..15689
                     /locus_tag="PK812_01640"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF001319.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HRJ68283.1"
                     /translation="MSRDRFLVGLLGANIAKSLSPALHEDAFDAHGLRGHYHLMDADR
                     IPFRRLEQALAAVKGLGFAGVNVTFPFKQAVVPLLDEVSADARQIGAVNTVTIDASGR
                     TAGFNTDRSGFRRSVEEAFGPAFVAGKRVVLVGAGGAGRAVAFALMDLKASHVAIHDV
                     DSQRATATVADLMTHYGAGCCSYAAHLADAIAGAAGVVNATPIGMLGMPGNPVPMEAV
                     RQHHFVADVIYTPMETALIAAARRKGCQVLTGGGMCVHQAADAFRLFTGLEPDVRRMA
                     TTFARALAQRDSALAKSA"
     gene            15720..>16765
                     /locus_tag="PK812_01645"
     CDS             15720..>16765
                     /locus_tag="PK812_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017988621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HRJ68284.1"
                     /translation="MKLSIATVCLSGDLRVKLEAIAAAGLRGVEIFENDLLTFDGTPS
                     DVRRMAADLGLEIVAFQPFRDFEGLPPVKRERAFARAERKFDLMAELGCSLMLICSNV
                     APDSIGGIARAADDFKALGERAVRRGFRVGFEALSWGRHINDYRDSWEVVRRADHPAI
                     GLVLDSFHIAARRTDMATIRNIPGDRIFLVQLADAPMLNMDYLTWGRHFRSFPGQGEM
                     PLADFMEALAATGYDGILSLEIFNDQFRAGSARPVAVDGHRSLIYALDHLATTTGRRL
                     PGLPQMPPRARCVGTEFIEFAVDARSAPQLERLLGGLGFRRTGRHRSKAVSRWQQGDI
                     NLVVNADANGFAHA"
     assembly_gap    16766..16865
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16866..17369
                     /locus_tag="PK812_01650"
     CDS             <16866..17369
                     /locus_tag="PK812_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRJ68285.1"
                     /translation="SKLPEAMVLDPGGVVKSQVIQTAGGELRLVLNASQSARTLSSRF
                     LSEAFGSGVQHIAFATDDIFATAAALRRAGVATLAIPENYYDDLETKTELSLDEINRL
                     KASGILYEQDECGEYLQIYTHAFDDRFFFEIVERRRAYQGFGASNAQIRLAAQTRIAR
                     DPAIPKL"
     gene            17457..18290
                     /locus_tag="PK812_01655"
     CDS             17457..18290
                     /locus_tag="PK812_01655"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HRJ68286.1"
                     /translation="MARRISLAHLTVLSLAPPEMIETAHATGYDAVGLRLIAVTPTTP
                     GYPLMSDPAMLRETKAALGRTGIAVNDIEFVRITPELDVRSLEPLLATGADLGARHLI
                     TAPYDEDLGRLADKLAAIADLAQPYRIMPLLEFFPWAFVPNFAGAKDLVAATRRDNVG
                     ILFDVLHFSRTGGSIQQLVSTPARLLPFVHLCDAPAAMPATMEAMLHTARCDRLPPGQ
                     GGLDLLPILSALPADCPLGIEVPMEVLTGEIGPQAVARRCRQATDRLLQTWAGMSADR
                     A"
     gene            18260..20026
                     /locus_tag="PK812_01660"
     CDS             18260..20026
                     /locus_tag="PK812_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRJ68287.1"
                     /translation="MGGHVGGSRLKREGWSVFAASDNAIACDLLVVGSGAGGLSAAVT
                     AAWHGLRVIVAEKEPVFGGTTAWSGGWMWTPRNPLARRAGFVEDIDAPRRYLRHVLGN
                     AFDEARVDAFLDAAPHMVAFFERETLLQFEDGNRICDVYGTVPGAGTGGRSVIAAPYD
                     ARQLGALVDKLRPPLRETAFAGLPIQAGADLAAFLSVTRSARSLAHVTRRVGRHLLDL
                     AIHGRAMQLRNGNALVARLLRSAADLGVDLRESSPVVSLRRGARGIEGATLSTPGGPV
                     HVSAARGIVLAAGGFPHDVARRGAVFPAADQHWTLAVPSATGDGLRLGEAAGGIVDTS
                     LAAPGAWCPVSLVPHPDGTTGRFPHIIERGKPGIIAVLADGRRFCNEANGYHDYVEAM
                     LRSVPRGLEMASWLICTRDFQRRYGLGIARPSPVPEGPYIRSGYIKTGRTLADLARAC
                     GIDPGGLERSVATYNEHARRGEDPQFGRGASPYNRLQGDATCRPNPCVAPIEKPPFYA
                     VKVVPGSFGTFAGLKTDAHARVLDRAGVPIAGLYASGTDMASIMGGYYPAGGINLGPA
                     MTFGYIAGRHAAGKDAVGFETG"
     gene            20187..20276
                     /locus_tag="PK812_01665"
     tRNA            20187..20276
                     /locus_tag="PK812_01665"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20221..20223,aa:Ser,seq:gga)
     gene            20711..21718
                     /locus_tag="PK812_01670"
     CDS             20711..21718
                     /locus_tag="PK812_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRJ68288.1"
                     /translation="MATLRSLASDLGLSITTVSRALDGYEDVAAATRERVNKAAAAAG
                     YRPNSTARRLRKGSSETVALVMPTEPGRFYEPVFVDLLSVLGERLAARHFDLMLLAAR
                     PGAEELAVYKRMIKDRRADACIIVRTRRHDERIAMLQEAGLPFVCHGRTETRAEYAYV
                     DGDGEAGFREVTNRIVSLGHRRIAHLAAPDHFTFAELRSRGWHMAMRAAGLTDDLKLM
                     CASTEEAGEQAATALLAGPDKPTALICATDRIAIGAVRAAQAAGLVVGRDIAVTGHDN
                     IHASRFTHPAITTMELDVRSVGACLADKLLHLVERGMPEHAGDVFPLRQVLRASSGEA
                     N"
     gene            21777..23057
                     /locus_tag="PK812_01675"
     CDS             21777..23057
                     /locus_tag="PK812_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007765679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRJ68289.1"
                     /translation="MTGLKRLLAATALTGAALSGAMAQETIFLSTQLRPVEEATKVRQ
                     IILKGAPGPVTFVVEEPSPFQTRMDAETQAGKRTISMVGALHGELAPLVGSKGLEPLD
                     ALVAKAKERGVPDAMLALGKLGTDKQMYIPWMQATYIMAAKKEALQYLPAGADVNALT
                     YVQLKDWAKNIQDKTGKRALGFPAGPKGLLARFFQGYLYPSYTGGVVASFKSAEAEAM
                     WGAFKDLWAHVNPNSASYDFMQEPLMAGEVMIAFDHVARLKDALVAAPNDYVTFPAPS
                     AAKGRGFMPVVAGLGIAPNAPNKAGAMAAIEHLLKPETQVTMARELGFFPVVKADLPT
                     DLPGGIKLLVEGVAKTQGAKDALVSLLPVGLGAKGGEFNKVYVDTFQAIVLRGAPIRA
                     TLDAQAATLQTIMNETKAPCWAPDKPSDGACKVN"
     gene            23117..23965
                     /locus_tag="PK812_01680"
     CDS             23117..23965
                     /locus_tag="PK812_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRJ68290.1"
                     /translation="MSRTRLLPYLLIGPATVFLLVLFVVPLVQTIALAFTDNGTASLT
                     NFSKMAGDLNFGLSLRNTFLLVLAVIPLQLCLALMMAMMVQKLEKGRDLVLWILIIPL
                     GISDLAAGLVWLSILQDRGYFNTVLNMLGLIDGPVNWLSYEAPLSLFIGVVLAEVWRA
                     TAIVLVILIAGLQLIPKEYAEAAEIFGATAWTRFRKITLPLLKPSIQSALILRTVLAF
                     EVFAVVYALSGRNFPVLVGEAYAWQHVNQNNGVAAAYAILVMGISLAATVIYIKALRI
                     RPEMQP"
     gene            23962..24786
                     /locus_tag="PK812_01685"
     CDS             23962..24786
                     /locus_tag="PK812_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015917991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRJ68291.1"
                     /translation="MSAASVSPGLRRLVFWSGVAMLCAWVLVPIYLIALGAFGGRLGV
                     FRWPKNLLPWDTSLAAMQTFLAVEGVWAAAWNGALVAGLTMLFSLLLGAPAGYALARY
                     SFAGQNAFRMLVVLTRAFPLAILALPLTVTFIRAGLYDTAIGVALVHTALALPFAALV
                     SSSLFMGIPRELEEAAWVFGCTGFQAFRKVVLPLALPGLAAAAIFAFVISWNEVFAAS
                     VLTVRNKTLTAYLLTVLAESPLHFRFAGGFLLILPSVIFIFAVRKYLFAMWGIANR"
     gene            24795..25853
                     /locus_tag="PK812_01690"
     CDS             24795..25853
                     /locus_tag="PK812_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ68292.1"
                     /translation="MAGITIDRIEKKFGTVTALQQLSLEVADSEFLALLGPSGCGKTT
                     LLRIIAGLESQTSGRILIGGRDVSALPPRQRGLAMVFQNYAVFPHMTVYENVAFGLRM
                     KGAGDAEVKKQVERAAGLLHIEPYLERYPAKLSGGQRQRVAVARALAVQPAVLLMDEP
                     LSNLDALLRLEMRSELKAVLSASGTTTVYVTHDQTEAMGLADRIAVMHKGVIEQIDRP
                     QTLYERPATRFVGGFVGSPPMNFIDLAVTAGKASLGAFSSSVALSEGTITAGIRAEDI
                     TVSDGGGMPFEVSVCEPLGATLLLTGRIEGQLARVSVRPDARVRPGDRLGLAPALDRI
                     VWIDPHNGKALGAATQSS"
     gene            25850..27115
                     /locus_tag="PK812_01695"
     CDS             25850..27115
                     /locus_tag="PK812_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017967643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HRJ68293.1"
                     /translation="MKMFNDTNAQAKAILAGNDRGGYTVPTHGLYPFQWNWDSAFVAM
                     GFATYDIDRALRELERLAEGQWDDGMIPQIVFHAPADSYFPGPEVWGTHHRIATSGIT
                     QPPAFAMALKFIADRAGAAFEPRIRALFAVADRYHRWFATARDPDGTGLVSTLHNWET
                     GRDNSPEWDVPFARVPETTATIIRRRDTGHVDASMRPTDADYRRYIHLVDLYRSLDWS
                     PAAMLEAAPFRVADIGTNAILLAADEALLELAPRFGASGSELDIRARISRLSHGLARL
                     WNAERGWYLSQDLLAMEPIAVRTSAGLLALLTTVPGRAQVDQMASAVAAHLDAVTMGI
                     ASTLPGEPGFEPKRYWRGPVWAIVNWMIAEGLARHGHAALAERLRAMTVSTIETRGFG
                     EYFDPTTGDALGGGAFSWTAAILLMLKAA"
     gene            complement(27361..28077)
                     /locus_tag="PK812_01700"
     CDS             complement(27361..28077)
                     /locus_tag="PK812_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1194 domain-containing protein"
                     /protein_id="HRJ68294.1"
                     /translation="MDVALVFAVDISYSMDPEEQALQKQGYVEALNSPEVLQAIRSGM
                     TGKIAVSYFEWANAFDQRMVVPWTIIDGPESARAVTDRIVASPLRRSQRTSVSGAIAY
                     GHKLIEAMPYRAFRKVLDVSGDGPNNNGDPVEVARDRALSAGIVINGLPIVLKRQTGY
                     GWGDIKDLDLYYQDCVIGGPGAFMVAIVSRDQFVPATRSKILREIADLGARDVQYAQN
                     DRRKSDCMIGERMYRERWGN"
     gene            complement(28355..29299)
                     /locus_tag="PK812_01705"
     CDS             complement(28355..29299)
                     /locus_tag="PK812_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018042418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRJ68295.1"
                     /translation="MTGIEPLAPLGEAAPETPAFNREAPVPGRLDQLSPLVRRLPVAN
                     PGPFTFTGTCTYIVGQGNVAVIDPGPADTHHIAALLEALGDERVAQIVVTHTHRDHSP
                     GAALLKARTGAPVVGCKPHMAFRDLHVGEIAMEAGADTAYRPDVALSDGEVLAGDGFT
                     LEAVFTPGHTANHLAFALKEENALFSGDHVMAWSTSIVAPPDGSMREFMLSLERLRGR
                     DETIYWPGHGGPVTEPGRFVRALIHHRRMRETSILKRLAAGDETIPALVDSIYEGLDP
                     RLKGAAGLSVLAHLEDLVERGAVVSDGPPLLGSRYAPR"
     gene            complement(29363..29764)
                     /gene="folK"
                     /locus_tag="PK812_01710"
                     /pseudo
     CDS             complement(29363..29764)
                     /gene="folK"
                     /locus_tag="PK812_01710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
     gene            complement(29768..30136)
                     /gene="folB"
                     /locus_tag="PK812_01715"
     CDS             complement(29768..30136)
                     /gene="folB"
                     /locus_tag="PK812_01715"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019903967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HRJ68296.1"
                     /translation="MTDRIRLTRIGVFAYHGLHPEETRLGQRFFISIDCRLDLRPAGI
                     ADDFTKTADYHAMTEQTQEVAVGRQFKTIEALAEAVAAMVLSGFPVVREVTVTVEKPA
                     APIPAMIENVSVEVTRQRTS"
     gene            complement(30133..30993)
                     /gene="folP"
                     /locus_tag="PK812_01720"
     CDS             complement(30133..30993)
                     /gene="folP"
                     /locus_tag="PK812_01720"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012317520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRJ68297.1"
                     /translation="MAFQPNTPNDNPWPQAAGGRTRIMGVINLTPDSFSDGGHVAGVD
                     QAVATAMRLADEGADILDIGGESTHPRATPIDAETELARVLPGLRAITDKVALPVSID
                     TYKARTADAALAAGARIVNDVWGLQRDGEMARVVAHHGAGLCIMHNRTTVDPSLDILV
                     DIRAFFERSLDMAARAGIPQARIVLDPGIGFGKTFDQNLIVLRRLAELKAFGLPLLVG
                     SSRKGFIGAVTGRSVPAERLPGTLAAHVAAALAGASIIRAHDIRPHREALALTDAIIR
                     GSVTPNQGTP"
     gene            31133..32236
                     /locus_tag="PK812_01725"
     CDS             31133..32236
                     /locus_tag="PK812_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRJ68298.1"
                     /translation="MDHTDCIVIGAGVIGLSIARELALAGREVIVLEAEKVIGSGVSA
                     RNSGVIHAGLYYPAGLAKARLCVEGKRRLYDFAQRCGLSHSRCGKLVVATAPEQNARL
                     EALKRQGEANGVDDLKMLTASEARTLEPELACVAALLSPSTGIVDVHDYLLALRGEAE
                     AHGAMIAFGTPALSGSAEDDGIRIETGGDSPMTLKGKAVIVAAGLQGQSVARAIVGMP
                     AERVPPLHLAKGNYYSLTGRQPFRRLIYPVPEDGGLGVHVTIDMGGQTRFGPDVEWID
                     TIDYSVDPCRADAFYAAIRRYWPALPDGALVPAYAGIRPKIERPGGSTTDFLVLTGKE
                     HGVPGLVTLFGIESPGLTASLAIAAEVAAAINP"
     gene            32248..33168
                     /locus_tag="PK812_01730"
     CDS             32248..33168
                     /locus_tag="PK812_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HRJ68299.1"
                     /translation="MADIVAYGEPMLEFNHTGEGGERLYLQGFGGDTSNFVIAAARQG
                     ARAAYVTALGDDRYGAMFRALWAEEGVDASGVRTDPDAPTAVYFVDHDASGHHFSFFR
                     KGSAASRFRPTDLPRALIGQARFFHLSGISLAISDTACDAGLEAMHLARQAGATVTFD
                     TNLRLRLWPKHRARAIITEAMRLAHVCLPSLDDIIAIGGPEQPDAIIDHILDLGPRTV
                     ALKLGARGAIIADAGKRAMLPPHPCRPVDATGAGDCFGGAFVARLAAGDDPVSAGRYA
                     GVAAALSTEGYGAVAPIPRASTVRAAMAGA"
     gene            complement(33056..34069)
                     /locus_tag="PK812_01735"
     CDS             complement(33056..34069)
                     /locus_tag="PK812_01735"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HRJ68300.1"
                     /translation="MANPEEVYVPDEARSGAVGHTPALAFFWRIRLLEILAAGIADLT
                     DPELPQPRRVQQGRRAAKATSALLRLAPPGLGRKARAARMVLASVRRSLGPSRDLDAL
                     AESFERIAPKGGGLNGKASRFVETWRERRLAANHEAVQSIGSAVEVLQRIRTHISGWK
                     PRSAADGAILAAVAQGYRRARRRMPSDLADPDLTEFHEWRKSVNDIRHQLAFLAAWLG
                     EPASHRLDRRSNRLRRLQVAIGEHRDLSLLHTELAGNGEGEALHRLLKRIDRKLEERL
                     KTCRKLAGRLFEERPRDLRDTLLSFRRRPSRLARSTRGESARPRHSLRSTAQPQPRRS
                     DRR"
     gene            34336..35193
                     /locus_tag="PK812_01740"
     CDS             34336..35193
                     /locus_tag="PK812_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRJ68301.1"
                     /translation="MRFLSFSVDGMPSYGIATSKGVHDLGLRHGNVFPDLRSFLEAQS
                     TGLLAPLSVPRETDYKLSEIEYDPVIPNPGKIICVGANYVAHREEMGRPEIGHPTLFT
                     RYADTLVGHKAGLVKPNATDRFDYEGELAVIIGQHCRNVPDSEAMDVIAGVCCFNDGS
                     VRDWQRHSSQFTAGKNFPGTGALGPEMVTLDELGDLGRLKLETRLNGQTVQSTSLDMM
                     IFSITASIAYITAFTPLAPGDIIATGTPGGVGDKRNPPLYMKVGDKVEVVIEKVGHLT
                     NPVVAEDEA"
     gene            35216..36853
                     /locus_tag="PK812_01745"
     CDS             35216..36853
                     /locus_tag="PK812_01745"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRJ68302.1"
                     /translation="MVTIYRARKIITMNPARPVATHVAVRDGRVLGAGSYEELKGWGE
                     HRLDERFATLVLMPGMVEGHSHLSEGVLWRHAYCGYFDRMDPDGKTWTGARSIEEIVA
                     RLRDAEAGMADPQRPVAGWGLDPIYYGNRRVMRADLDQVSTRRPVGVVHASGHILNVN
                     TRGLELAGLMRAGINHPGVVLGADGLPTGELKGPEAMSLAARHIGMDRGLLNSDEAGL
                     RAFARLCVRTGVTTAADLGNMLEPQAVEMMTRVTAEERFPARIVALRMAFDIEPEALI
                     ARAREVSGMSTDRLRLGIIKVVVDGSIQGFSARLLWPGYYNGAPNGLWYIAPQRLAEI
                     FERALAAGIQVHMHTNGDEATELVLDTMEAALRKHPSADHRFTLQHCQLANRAQFRRM
                     KALGMNVNLFPNHHFYWGDEHYALTVGPERAERMNACASALEAGVPFAIHSDAPVTPL
                     GPLFTAWCAVNRRTASGRTLGEAEKIPVGEALRTITLGAAHTFKLDAEIGSIETGKRA
                     DFAVLEDDPEHVGAELLKDVRVWGTVLGGRVFAAADI"
     gene            36850..37776
                     /locus_tag="PK812_01750"
     CDS             36850..37776
                     /locus_tag="PK812_01750"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRJ68303.1"
                     /translation="MTGRIPVTVIGGYLGAGKTTLVNHLLRHAGGLRLAVMVNDFGAM
                     AIDADLIEAQEEDLISIAGGCICCSFGSDLIEAMAKLAERSPRPDHVLLETSGVALPG
                     PVAGTVSLLPDFDVDCVAVLADATRIRALVADRYLADTMDRQLADAQIVLLTKTDCIG
                     PSLADETLAWLSGRVPGARCLPVSNGAVDPAVLIGARLGYRATLSLSAPHQAAMLYHT
                     ESLPMVEPLDIAALTTLLQGADLGLLRAKGFVRNAVGQIYGVQVVGTVVRVERHLAPR
                     PVEPGLIAIGLRRSFDPALFRQALAVTRVTGD"
     gene            complement(37763..38122)
                     /locus_tag="PK812_01755"
     CDS             complement(37763..38122)
                     /locus_tag="PK812_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019905637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HRJ68304.1"
                     /translation="MSVRVLLLIASMFGASGVALLAAGAHAAGNQATTAGQMLLFHAP
                     ALMAGALALATGRLRAGLALPALVVMALGVALFAGDLTLRALHGMRLFANAAPIGGLL
                     TISGWIALAVAALVNRR"
     gene            complement(38147..40195)
                     /locus_tag="PK812_01760"
     CDS             complement(38147..40195)
                     /locus_tag="PK812_01760"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRJ68305.1"
                     /translation="MPSPKREVTGAYEAWAYGIGAPHVFGTSQAREAPFIAALAALPA
                     GMRPEALAKGTRRDTQRRVLVPQLWNGAHADGDVAFWPVILEAASRVAGANGLVDTYA
                     GALRGTKGLREETGLRLNMPVPSRAVDTAYDPRRIERRLGQHPPAKPKVVVAVIEDGI
                     PFAHKALRSADGERSRVAFCWMQSASTAETAAQRTVLFGREFLAPDIDALLTMAGGDE
                     DLFYDLAGARTENAYPYATLDRRGSHGSHVTGLAAGQDGAAMDDVAVIAVQLPEPLTL
                     DTAGVGKDAFILSALHYIFDRTDRLAGAHGIDRLPLVVNFSYGFTGGPHGGRDRLERA
                     IRTLVAKRAATQPTHLVMPAGNTFHARLYGEISAAMLSARGHKPDAFTIPWRIQPGDR
                     TSNYLELWLPDRASPAGVTIDVALPGGSEHDLSWTLGDGERLATRPLKSRGRDVGEAS
                     IERYGIGSGASLWRLLLAIGPTEPTDPLAPAAPAGLVRIGLSGIGAVLACGPICCRIQ
                     RDTDPFGYQRGARQSYLDDPYDGVLRANGAPADAENPPAAFVRRFGTLNGLATHDRVT
                     VVGGFVGQTRKPSVYASGPSFASPGLPFSAPSDSNPVLRGVPGHGTRSGAVIRMDGTS
                     VAAPQIARALARRYVEQPELADKLTASIGYLIGGSLESPASSASQTPQDRQRLGRRLR
                     "
     gene            complement(40237..41364)
                     /locus_tag="PK812_01765"
     CDS             complement(40237..41364)
                     /locus_tag="PK812_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRJ68306.1"
                     /translation="MTWMGGIGGIGGIGGIGGIGGIGGIGGIGGIGGIGGIGGFVSGG
                     SGDPAVSTGLMTSAQAILHVGDEGVAAADALAFSSSANMTLWAGWVRASLYLTDFLKD
                     LSWSARPKDGGSIEIRYAGNRLVELARPDPALFQRQVELVRSYMDQRADRQVEILTQV
                     GFPLDYFASILGLNASTNPRTFELILITQVLTAHVAMVAKHHLACRRPDRLSGTIMPL
                     IPTPGHGAFPSAHAAESFAAATLLSTLLSEADVTRHYPDVTNRRKLLFKQAERIAVNR
                     TVAGMHFPIDTWAGAALGEAVAQAILSKCGKGAPIPRSYAARDIDFSVARFAPEIWGG
                     RPASPAETGLRRDRRPAKGKIEPSDHFQWLWNEAVSEFKLP"
     gene            complement(41389..43032)
                     /locus_tag="PK812_01770"
     CDS             complement(41389..43032)
                     /locus_tag="PK812_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68307.1"
                     /translation="MVGKVLDVNLFGACSVESATPGRFCITGTKQKALFALLATAPFG
                     RRTRAFLQETLWGASCYDTGRQSLRRALSDIKQIMGEAYSEVLSSNNLELTIDLSRVR
                     FMGHLQAGLFLEGIEIREPLFNEWLVGIRNNPGQLAGLFSLTDRPHVSRILPGVAVLP
                     FRAIGGDRSDAVFGDWIAEELSRTLSRSRLFDVISHLSCRQVDSSGVDMSHVRDLLKA
                     DFCVSGSLRRTRSEIVLDADLIDARSGRILWTRQFLNPYDAFLDRSGEALAAILTSIS
                     GAIADDALTHVIGRSPPEIDDHRLLIAGIGLMHRATLRQFMRSHDLLNEAARRAPDAP
                     EVHAWLGKWCVLSVFNGWSTDIAKDTRLAVDHTARGLDLSPDNAFCLTIDGFAHSNLL
                     RRLDIAEKRYDLALAANPNSSLTWLLKGALHAFRGESTNAIGATDRARRLSPLDPFGY
                     FYDSLDATARLAHGDYRSGLELANRSLAANDRHLSTYRAKIIALYGLGQIAEAKIVGG
                     ELLRRQPDLTVAGYISSHPAADHDFGRLAAKALTAAGIP"
BASE COUNT         7245 a        13926 c        14182 g         7912 t
ORIGIN      
        1 acccgcacgc tcggcccgct gcttccggtg cacagggtcg cgctcgcaaa catcgccgcc
       61 gcgtttccgg aaaagcttga agccgaacgg cgcgccattg cgcgtggcgc ctgggagaac
      121 ctcgggcgcg tcgccgcgga gtatgcccat ctgcaccacc tgttcgactg ggacgcggac
      181 cagccggaca agccctcgcg ctgcgcggtg gaaggcatcg agcatttcat cgcgctgcgc
      241 gacgacaaca ggccgggcat catcttttcc gcccatctcg ccaactggga actgccggcg
      301 atctgcgctg cgacctatgg cctgaacgtc acggcggtct tccgcgcgcc gaacaatccg
      361 gccgtcaacc gcgtggtgca tgccgtccgt tccggcacga tgggcgggct ggaggcggcg
      421 cggcagggcg cggccttcgc catgaaggac gcgctggaga agggtggtca cctcggcatg
      481 ctgatcgacc agcatttcac gcgcggcgtg gctgtgccgt tcttcggccg gcccgccatg
      541 accaatccga tcatggcgaa attcgccgaa gcctttgact gtccggtgca tggtgcgcgc
      601 gtgatccgtt tgcccggcta tcgtttcaag ctcgaactga ccccgcccct ggagctgccg
      661 cgccatccgg acgggtcatt ggatgttccc ggcgcgatgg cggcgatgac ggccgtggtc
      721 gaaggctggg tgcgcgagca cccggagcaa tggctgtgga tgcaccggcg ctggcgcacg
      781 ccgacgaagt agggggctga agcttgccgc atcggccacg aagcgcgtat gaacggccgg
      841 cgcaggtcag agtccggaaa ccgctcgcga tgcaacagac gccagatccg gttccggaag
      901 gcagggccgc tgccgaggcc gctcctgcac ctgtcagcga gaccgccgcc ttccgccgcc
      961 gaacggcgat gcagcgcctt ggcgcgctcg ccagcgccgc gctgttcatc atggcgctgg
     1021 tcgtgctgta cctcgtcatt tcggagcttg atttcgatga ggtgaaagcg gtcttcgggc
     1081 gaacatcgcg cgagcagttg ctgatggcct tgggcctgac cgtgctcagc tacctgatgt
     1141 tgaccggcta cgatgcgctc gccctgtggc aactggcgaa aggccaggtg cgctaccggc
     1201 tgaccgcgct ggcttccttc accagcttcg ccgtcagctt cacgctcggg tttccgctga
     1261 tcaccgccgc gaccgtgcgc tactgggtct actccgccgt cggtctcggg ccaggcgtca
     1321 tcgccagtct gaccctcatc gccgggctca ctttctggct cggcatgagc gttgtgctcg
     1381 gcctggtgat gatagcgcag ccctttgcga cttcggtgtt cacgcgcctg ccggctcagg
     1441 tcaacggcta tgtcgggttc gccattctct ctgcgatctt tctctacacg gtctggatcg
     1501 ccgcccggcc gcggcatatc cgcatccagg gctggacgct gcacctgccg cgcctacgcg
     1561 tgagcctgct gcagatgttg ctcggcgcgg tcgatgtctg tgcggcctgc ggtgtgctgt
     1621 acgtgctgct gcctgccggg cacggcgtac ccttcgcggc gatggttgcg gcgttcgtct
     1681 ttgcctgcgt gctcgggatc gccagtcatg cgccaggcgg cattggcgtc ttcgaggcaa
     1741 cgctcctgct tgcgctgccc ggaatcccga aggaggcgct gctgggatca atcctgatgt
     1801 accggctgtg ctattacgtt ctgccgttca tcgcggcgat ctggctgctg ggcctgcggg
     1861 aaatctggct gcgcgcgcga cgattgcgca gcgagatcag cgggagctga ggcagtggcg
     1921 ggtcgctggg tccttgccgg tgccatggcg atcgccgcta tcctcttcgg cgtggcgatt
     1981 gacgggtgga gtgggctgct gatcggtacg gttgcgctgg gcgccggtgg ggtcgcggca
     2041 aagctgcttt caggccgggg tgaacagtct ggtgcgtctg ccggccacca gtcggcgcag
     2101 ccgggcaatg ccggcatgca ggaggcgcta gaaactgcgc cgctggctgc cctgctgctc
     2161 gcccgggaca cgaccatcct gttcgccaac cgcgaggcgc gccggctgtt tccatccctg
     2221 cggaccggcg accctctgtc cctgcgtctg cgcgttcccg aggtgcttga tgcggttgca
     2281 gcgaccctgc gcgaccatgt ggcgcagggc acggactttt ccgagcgccg tcccgtggaa
     2341 cggtcctttc atgccaggac gttcccgctg acggccgaat ccgggaaagc tccggcgctg
     2401 gcggtctttc tggaagacgc gtccgggctc aggcgtgtgg aggcgatgcg ctccgacttc
     2461 gtcgccaacg ccagtcatga actgcgcacg ccgctcgcct cgatcctcgg attcatcgag
     2521 accttgcagg gaccggcgag aagggatgcc gaggcgcagc agaagttcct ggccatcatg
     2581 gctgatcagg cgcggcgcat ggcccgtctg gtcgatgacc tgctgtcgct atcgcggatc
     2641 caactgaagg aacatatccc gccaaccgcg cgggtggacg tccggggcgt tgtcgagcag
     2701 atcgcggatg cgctttcggg actggcccgg gagcgtggcg tcactgtcgc gatcatggcg
     2761 agcggcccgg cggtggtggc cggagaccgc gacgatctgc tgcgcgtctt cgacaatctg
     2821 atcgagaacg ccatcaagta tggccgctcc ggcggcaagg tcgacgtctc gatcgaggac
     2881 gaggccgcaa cgccggacat cgtcatccgc gttcgcgatt acgggccggg gatcgatccg
     2941 cagcacctgc cacgcctgac ggagcggttc tacagggtcg atacgacggt aagccgggcg
     3001 gaaggcggca ccgggctcgg tcttgccctt gtcaagcata tcgtcaaccg gcatcgcggc
     3061 aggctcgaca tcgacagcga gccgggcatg ggcgcgacgt tcagcgtcag tctgccgcgg
     3121 ccgaaatgac gacgcgcgaa ccgtcatcaa tctgttatgc gcttgtcgta tttcgccact
     3181 gcatgaaaat ggcagggagc gacccgacat ggcggcgctg atctggatcg tcgaggatga
     3241 agaggcgctt tcgctcctgc tgcgctacaa tctggaggct gaagggtttg ccgtcgaggt
     3301 catcacgcgc ggcgacgacg ctgatgtccg gctgaaggaa cagcagcccg acctcttgct
     3361 gctcgactgg atgctgccgg gcgtttccgg catcgagata tgtcggcgct tgcggtcacg
     3421 cgacggcggc gagcgcttgc cgatcatcat gctcacggcc cgcggcgagg aggccgagcg
     3481 cattcgcggc ctcaagaccg gcgccgatga ttacatggtc aagccgttct ccattcctga
     3541 actcatggcg cgcgtacggg cgctgctgcg ccgcgccgcg ccggagcgcg tgacgaccat
     3601 gctcaggatc gcggatctgg aggtcaaccg ggaaacccga cgggtgcggc gtggcggaaa
     3661 ggaactgcac ctcggcccca ccgaataccg gctgctcgaa ttcctgatga gccgccccgg
     3721 ccgcgtcttc agtcgcgagc aacttctcga tggcgtctgg ggatcggaga cctacatcga
     3781 cgatcggacc gtcgacgttc atgtcggccg cctcaggcgc gcgctcaaca cgacggacca
     3841 gcgcgacccg atccggaccg tgcgtggcgc cggttactcc ttcgacgaga cgttcggcgc
     3901 tgcgggctga attcagcgcg gcgcgagcct gcggcggtcc tgggatggct ggaaggcgac
     3961 ccgggcatgc gccatgcaat acggcttgcc gacctcgcag tcgccgccgc agaagacgaa
     4021 atccggctgg agcgggtcgc ccatcggcca gcggcaggtg ccttcccgga gttccatgat
     4081 cgatatgcgc cgcgaaatcg gaaccaccac ctcctcggcc ggccggtagg ccacgacggc
     4141 ctcggtgtcg agcaactgca cgacggccag gttaccgcgg gcgatggtcg tcacgcgcgg
     4201 ggttgcccgg acctgcgacc ggaccgtctt gcgactgcgt tgggctgtcg tcgaggacgt
     4261 cttcacccgt ccggacaggt gcaaccggtg caccttgccg atcacggcat tgcgcgtgac
     4321 gccgccgagc tcggctgcga tctggctggc gctcagccct tcgctccaga gcttcttcag
     4381 gcgttcgatc cgttcatcgg tccaggacat gaccttcctc tcaagacata cccgtttgtg
     4441 aatccatcgc cgtccggcgc gatcccgttg caccggccca accactcacg cacagactga
     4501 aattactgaa gcgccacgac atctcgtggg tgacgcagac aacgctacta gatcggggag
     4561 cgtagcggca gattccttca gcgcttcact tcagttttcc ccaacgatct cctgtgggaa
     4621 agatggcctc aggtcaagag tccggcttgc gcgatgcaat tgaccagaac ggccaaaaat
     4681 gggaaaagaa cgcgccgccg ccgaaatgcc ggcggctttt tgcgttcggg gcccctgcgg
     4741 accccagatt caggaacgga ccatgaccaa ggctggtggc gaaaaaaaga cagaatcggc
     4801 agtcctgccg acctacgcgc gcgctgacct ccggttcgag cgaggcgagg ggtcctggct
     4861 gatcaccgat accggcgacc gctacctgga ttgcgtcggc ggcgtcgcgg tgaacgcgct
     4921 cggccattgt catccgcatc tggtcgagac gctgcagaag caggcggcca ggctctggca
     4981 tacgtccaac ctcttccgca tcccggaagg cgagcgcctc gcccgccgcc tggcggacct
     5041 gacgttcgcc gaccagatgt tcttcacgaa ctcgggcgcc gaggcgctgg aatgcgccat
     5101 caagatggcg cgcaagtacc atgcggtcac gggtcatccc gagcgctacc ggatcatcac
     5161 cttcgaaggc gcgttccatg gccgcacgtt ggcgaccatc gcggccggtg ggaacaagaa
     5221 gtacctggaa ggattcgggc cggagatgcc gggtttcgat caggtgcccc tgaacgacat
     5281 cgacgcggtg aagaccagga tcggcgccga gacggccggc atcctggtgg agccagtgca
     5341 gggcgaaggc ggcgtgcggt cggcgacccc ggccttcctg cgcgagctgc gccagatctg
     5401 cgacgaaaac ggcctgttgc tgatcttcga cgaggtgcaa tgcggcatgg gtcgcaccgg
     5461 gaagctgttc gcgcatgaat ggcccggtat cgctccggac atcatggcca tagccaaggc
     5521 gatcggcggc ggtttcccgc tcggcgcctg tctctgcacc gcggaagccg gcaagggcat
     5581 gaccgccggc acgcatgggt ccacctatgg cggcaatccg atggccatgg cggtcggcaa
     5641 cgccgtgctc gacgtgatcg cgcaggacga gtttctggcg gaggtgcagc gcaaggccct
     5701 gctgttcaag cagcgtctgg cggagctgaa ggaccgctat ccccgcatca tcgccgagat
     5761 tcgcggcgag ggcctgctgc ttggcctcaa ggccgtcgtg cccaacaccg acctcgtcgc
     5821 cgccgcgcgc gagcagcatc tgctggccgc cgccgccggc gagaacgtga tgcgcctgat
     5881 gccgccgctc aacatcaccg atcaggacat cgccgaagtc ttgaagcggc tcgatgccgc
     5941 ctgcgccgcg ctcgaaggca aggcgcggtc cgccgcctga ggaaagggca agtatcatgc
     6001 gcatgaccgc tctccggcat tttctcgatc tctccgcact caggccggac gagttcaccg
     6061 gcatactctc ggcagcagca cgcatgaaga agcgccgcgg tacgccggca gcggaccagg
     6121 atcggccgct ggctggcaag aagatcgcca tggtgttcga acagccctcg ctgcgcacgc
     6181 gcgtgtcctt cgacgtcggc atccgcgaac tcggaggcga gccgctgatg atcaccggca
     6241 aggagattga actgggcgag cgcgagacca ttgccgacac ggcgcgcgtg atgtcgcgtt
     6301 atgtcgacgc catcatgatc cgcatgctgg atcacgcgca gctctacgaa ctggccgccc
     6361 atgcgaccgt gccggtgatc aacgggctca ccaagcgctc gcacccctgc caggtgacgg
     6421 cggacatcat gaccttcgag gagcgcgtgg gtccgctcgc cgggcgtacc gtggcctgga
     6481 ccggcgacgc gaacaacgtg cttgcgtctt gggttcaggc atcggcgctg atcccgttca
     6541 ccttcaggat cgccacgccg gaagaactgg cgccccggcc cgagctgatc gccgaggcac
     6601 gcaaggcagg cggcaggatc gaagtcggcc acgacccgat caaggcagtt gaaggcgcgg
     6661 actgcgtcat cacggactgc tgggtctcga tgggtgacaa ggacgagggg cgccggcaca
     6721 acttgctgcg cccgttccag gttaaccgcc ggctgatgag cgccgcgaac cagggcgcga
     6781 tcttcatgca ctgcctgccg gcaacgcgtg gcgaggaagt cacggatgaa gtgatggacg
     6841 ggccgcaatc ggccgttttc gacgaggcgg agaaccgcct gcatgcccag aaggcggtgc
     6901 tcgcctgggt ttttggcgca gcggtttcct gaagatccgt catgaacgag cccctgaccc
     6961 ggttcgtctc cgatgcaccg ggagccggag acgacgccgt gctgcccttc caggtggatg
     7021 cgctggatgc gcgcgggcgc cttgtccgtc tcggtccggt tgtcgacacg atcctgcatc
     7081 gccacgccta tccggcccag gtcgcccggt tgctgggaga ggcgatcgcg ctgacggcgc
     7141 tgctaggcgc gtctctcaag ttcgatggga aattccagct tcagacccgc acggacgggc
     7201 cggttggcat gctggtcgtc gatttcaccg cgccgtccag cctgcgggcc tatgcgcggt
     7261 tcgaggcgga caggatcgtg gcgggggccg ggaccgcttc cctgctcggc aacggacatc
     7321 tggcgctgac gatcgaccag ggtccgcata tgcagcgcta ccagggtgtg gtcccgcttg
     7381 acgggcaggg gctcgaaacg gcggcgcacc agtacttcct gcgatcggaa cagatcccca
     7441 cgaaagtgcg cctcgccgtt gccgaagaaa ccacgccgac cgacagcgga ccgcgcgcgc
     7501 gctggcgtgc cggagggatc ctgattcagt tcctgcccaa gtccgcggac cgccagcgcc
     7561 aggccgagct ggaccccggc gatgcgccgc ctgggactgc ggcggcgccc tttgccgaag
     7621 acgacgcctg ggtggaggcg cggtcccatg tcgagaccgt cgaggatcac gaactggtcg
     7681 accccgcgat cagcggcgag cggctgctgt atcgcctgtt ccacgagcgc ggcgtaaggg
     7741 tgttcgagcg gaatccactg gtcgagcgct gccgctgttc ctcgcagcgc atcctggaca
     7801 tgctgaagag cttctcagcc gacgaccgac aggcgatggt cggcgacgat ggccgcatcg
     7861 gcgtcacctg cgagttctgc tcggtgcact acggtttcga tccggctgat gccgaaagcg
     7921 ctagttgagc tggcgcttct cgaaggggct gtcgagcgag aaggcgggaa tctgcacctg
     7981 aaatcgccgg ccttgtgcat ccaccatctc atactctccc accatgatgc cctggggcgt
     8041 ggaaagcgga cagccggacg cgtagcggaa cgtgtcgccg ggcttgagaa tgggttgttc
     8101 gcccaccaca ccggggccgc gcacgtcctg gcgttgaccg ttgccgtcgg tgatctccca
     8161 gtgcctgtgg cgcagctgga cggtctcgtc gccgtgattt gtgatttcca cgtcgtaggt
     8221 ccagaagtac tggttcttct cgggctgcga ccggtcctgc gaaaactgcg ggcggaccac
     8281 gacctcgata tcgtcggtaa cggcgcgata catgctggtt cagtccctgg actgcctccg
     8341 gcggacactc tgcgtattgt cgggcatcgc ctgcatttgg caagcctgcc cgctggatac
     8401 aggaaaggaa ttgcatgctg gatggcgtga tgcgccggat cgtgacgccg ccgctggacg
     8461 ggatcgcggc gtggtgcgcg gcgcgaggga tcgcggccaa tgccgtgacg ttgcttggcg
     8521 cggccttcgg cctcgcctgc ggtgcctgca tcgccgtcgg ctggttcatg ccggcgcttg
     8581 cctgtctggc ggcgagccgg attgcggacg ggctggatgg cgccattgcc cggcgcagcg
     8641 gaatcacgga tttcggcggc tacctggata tcgtctgcga cttcgttttc tactcgagcg
     8701 tgccggtggg tttcgccatc ctcgactccg gacgctgcgc cgtcgccgcc tcgctgctgc
     8761 tggcgagctt ctatctcaat ggcgcgagct tcctggggtt ttcggcactg gcagccaagc
     8821 gcggcatgga cacggatgtc cggggccgca agtcgcttta ttttaccacg ggcctcatgg
     8881 agggcagcga gaccatcgcg tttttcgcgg ccttcctgat ctggcctgag tactttcccg
     8941 tgctggcgcc gatcttcacg gcgctgtgcc ttgtcaccat gttggcccgg atggcgctgg
     9001 cctggcgcgt cttctcggtg gaagactccg gtcgccggca aggctgagct tgccgccaca
     9061 ttccctgacg actagggctt tcgaaggagg atttcagcgc gtgacaggca gcagcggcat
     9121 ccattcggac acgatgatcc tgggactgat tcagtccggc accatccagc tggcagcgcc
     9181 cgtggcgacc gggcaggtcc agcccgccag cctcgatctg cgtctcggcg cccgttgttg
     9241 gcgcatgagg gccagcttcc tgcccggtcc acagcgacag gtgcgcgagc gggtggacgc
     9301 gctggcgctg catgagatcg acctgaccaa gggcgcggtg cttgaaaccg gctgtgtgta
     9361 tatcgccgaa ctcatggaaa gcttggcgct gcccaaaggg gtttcggcct ccgcgaaccc
     9421 caagagttcc accggccggc tcgatgtctt cacccgtgtc atcaccgacc ggggcgctgc
     9481 cttcgatgtg gtgccggagg gctatgccgg gccgctctat gccgaaattt cgccgaagac
     9541 ctttcccgtg ctggtgcgca ccggctcgcg cctgtcgcag atccgcttcc gctccggcac
     9601 gccacgcctc gacgatgccg aattgtcggc gctgaacgca cgcgaggcgc tggtcagcgg
     9661 tggccggcat ccgattgccg ggggcgtcgc ggtcagcgtc gacctttccg gtttcgacgc
     9721 cggcggcctg atcggctacc gcggaaagcg ccacaccggc cttgtcgatg tggacagggt
     9781 gggcggctgc cgcatcttgg actactggga gccgctgacg gcggatggaa ccggtgcgct
     9841 cgtgctggac ccgagcgagt tctacatcct tgcgtccaag gaagccgtgc atgtaccccc
     9901 ggattacgcg gcggaaatgg tgcccttcga tccactcgtt ggagaattcc gcgtgcacta
     9961 cgccggcttt ttcgaccccg gtttcggcca tgccggcgcg ggcggggccg gctcgcgcgc
    10021 ggtgctggag gtgcgctcgc acgacgtgcc tttcatcatt gaggatgggc agatcgtcgg
    10081 gcgcctcgtc tacgagcaca tggcggaacg accggcgacg ctctatggtg ccggcctcag
    10141 ttccaactat caggcgcagg gcctgaaact gtcgaagcat ttccgctgac gggttgcggc
    10201 gggctggcga accggcttat atcgcggtgc gggcgggcgt agttcagtgg tagaacgaca
    10261 gcttcccaag ctgtatgtcg tgggttcgat tcccatcgcc cgctccagat catcccggat
    10321 atcacgacga cggaacacgc tggagcgcac ttgggccggt tgcggagcgc gccttgtgcg
    10381 cccgaaaacg gacctgatcg catccgtgag cttccaccag ggccagcgcc tgaatcgcct
    10441 gtgggcgctt cgggcagcgc cgccgtcgcg cgatacttcc tggcttgtcg gcagaagtgc
    10501 cgccgacgat ccctgggcgg cgtcggtgcc ttgatcgtgc cgatgcagtg ttgaccagcg
    10561 acacctgtcg tttcagcgct atgctggaac cggtttcgaa ggagtcgttc gattgcttac
    10621 cctccgccag atcgaggtca tccgcgccat aatggtgacg ggcacggtcg gcggcgccgc
    10681 gcggctgctg cacgtctcag cgcccggcgt cagccgcacc atgaagcacg ccgagagcgt
    10741 cgtgggcatc aagctgttct cccggaaggg cgggcgctat acgccgacgc gcgaagcctc
    10801 cgacatcttc ggccagatca acgccgtcta tgacaaggtg gaggatctgc agttcgtcat
    10861 cgatcgcatg aagaagggcg aggaagcgga actgaggatc ggatcggtgc caagcctgtc
    10921 gaatgtgatg gtgccacgcg ccattgccga catccggagc gcctacccga gcctcaggat
    10981 cgatttcgat gtgctcaaga tcgaggatgc catcgactat ctgctgctgg gaaagggtga
    11041 agtcgtggcg atgagctaca ggatcgagca tccgatgctc tcgttccagc cgctcgccac
    11101 ggggaggctc aaatgcatcg tcccggccgg ccatcctttt ggcggaagga agcgcatttc
    11161 cgcgcaagag attgcggatc atccgctgat cggcatcgac ccgaacgatc cctacgggcg
    11221 catcatggcc ggtatttttg cggcaagtgc cgcgaagtat gacgtgacca tacgggcgcg
    11281 cttcggctcg accgtttgcg cgctggtgac caacggcctt ggcatcgctg tcattgatga
    11341 attcactctg gccggaggca actggcctgg ccttcgcgcg ctcgacattg aggagccgac
    11401 cgcctttcag acctacattg ccatgcgccg cgatgccgcg ctcaccgggt actgcgaagc
    11461 gttcgtcaca aggctgcgcc atcacatgca ggcgctgtca ccgggaaagc cggcgttggg
    11521 tggggtaggc cgcagcggca aaaattaaca tcatgtttgg cgcaaaagca ttcttggtat
    11581 ttgaccgcat cttgggcttc tgccaactct agcgcgcctg ggtcatctgc gagcggcgca
    11641 gcccgaaaca gcgacagaga gccgggcgtg gaggaactca gggagatcgc atgatgcgtc
    11701 acatgttcaa actggcagct ttggctgccg ccttggccgc ttttgcagcg ccggcgtccg
    11761 cgcagcctta cccaaaccag gatatccagg gcgtgatcca gtggggcgcg ggcggcagca
    11821 cggacgttgt ggtgcgcgct gtcacgcccc atgccgagaa ggttctcggg cgttcggtca
    11881 tcatgaccaa ccgcaccggc ggtgtcggcg cgatcgcgat gaagttcgtg cagtcccaga
    11941 agtccgacgg ctacaccctg ctgatgggcg cggagaaccc gcaactctac aaggtcatgg
    12001 gcctttccga caccgactac accgacagtt tcaccaccat caatctgctc gcccgtggca
    12061 ttccgatcct ggtggccaac aaggatgcgc cgttcaatac ggcgaaggaa ctcgaagcct
    12121 atgccaaggc aaacccgggc aaggtcaagg tcggctcgac cggtcccggc ggcctgcctt
    12181 cggtcgtcat ggccatgctc ggtacgcagg tgaagcttga cctgactgcg gtcccctatg
    12241 acggcgacgg cccggccctg accgccctcc agggcggcgc catcgacctg atgccggccg
    12301 ttcttggcgc cgcgatcgaa catatcaagg ccggtcgcat gaagccgatc gcggtgatcg
    12361 acaccgccgc gaacccggca ttgccgaacg tgaagccgat cgtcgcggat tatcccggct
    12421 tttccaagct gctgccctgg ggtcccttct tcggcatctt cgtcaagaag gacacgccga
    12481 aggatgtcgt cgacaagctt gtccaggcct ttaatcaggg tgccaagcac ccggacttcg
    12541 tcaagctgat cgacgaccgc ggcttcaaga tgatgaacat cgccggcgcc gaagcggatg
    12601 acttcctgaa gcggtggcag gcaaacacat cgtggatcgt gttcgatgcc ggcatcgcca
    12661 agaagtcgcc ggccgagttc aacattccga agccctgatc tgaacaaggg gcggcaaagc
    12721 gaagggcttt gccgcctttt cctcttcaca ggctaaaacg gaacgcgcca tggatccgca
    12781 acccacgcgg cgcccggggg agaccgtgtt cacggtcatg atgctgctgc tcagcatctt
    12841 cctcctgtgg aacgcctaca agatatccgg attttcgtcg aagagttcac ccggagcgtt
    12901 tcccctcgct gcgaccctcg tcatggtcgt cgccgggctt gtggccgtgt tcaacacgct
    12961 gcgcctgcct gagggcccga agggcttttc cgccttccgg tcgcaggtcg cacccaacct
    13021 cgtccttgcc ttcgccggcc tgatcctttt ctacggcctg gcgctggagt cggtgggatt
    13081 catcctgacc tcgctcgcct tcctgtttgc cggcatgctg ctgctgcaca ggcaaggtat
    13141 tggccggtcg ctgatgtatt ccgtgctctc gcttgcgctc gtctacgtga ccttccggct
    13201 cgtcttcaag gtcgtcctgc cggagggcat catcccggag cggcgcatcc tggccgagat
    13261 tggtgccgtc cttggcccct atctgggggt gcggtgatgg aagcgctcaa gtatttcacg
    13321 atggcctggt tcgatccgaa ccttctgttc ctgacggcca tcggcacctt tgccggaatc
    13381 tatatcgggg caatccccgg cctttccgtg acgatggccg tgtcgatcct gatcggcttc
    13441 accttttcct gggaagtcaa cagcgcgctc gcgcttatcg ccgggatctt caccggcggc
    13501 gtctacggcg ggtcgcgtac cgcgatcctg ctcaacattc ccggcgcgcc cgccgcgatc
    13561 gccaccggct tcgatggcta tccgctcgcc aagaagggcg aggcaggcaa cgccatcggc
    13621 atctcgacgg tcatgtcggt catcggcggc ttctttggca tcctcgttct ggccgtcgga
    13681 gcaccgatca tctcggagtt cgccgtcagg gtgcagccgc gcgactacct gcttctggcc
    13741 ctcatgggcg tactcctcgt cggcgctctg tcgggcgaaa gcctggcgaa aggcatctgg
    13801 gccgcagcct tcggcatggt tctcggttcc gttgggctcg atccgttgac ggtcgaggag
    13861 cgcttcacct ttggcctgcc gcagttgacc aacggtatct cggcgatcgc cgtgatgatc
    13921 ggcatgttcg gcctgtccga ggcgctgatc ctgctgcacg acctcgacaa gaaggcaatc
    13981 aagcaacgcg tggacaacat cgttccgaag tggtcggaca tcaagaagta cttcggcctg
    14041 tcgatgcagt cctccgccat cggcgtcacg gtcggggcct tgccgggtac gggcggcgac
    14101 atcgccgccc tgcttgccta tgaccatgcc aagcgcgtga ccaagaaccc ggaagtgccg
    14161 ttcgggcaag gcgcgaagga aggcatcgtc gcgccggaag cggcaaacaa tgcggcggtc
    14221 ggagggtcct atatccccat gctcaccctc ggcatccccg gcgatgcggt gacggcggtc
    14281 atcatcggcg ccctgttcat caagggcctc aatcccggcc cgctgatgct gatcgagaag
    14341 ccgcatatgt tctggttcac ggtcggcaac ctgacgctcg ccaactgctt cctgctgatt
    14401 ttcgggctga ccggaatcaa gatcttcgca aagatcgtcg aaatcccgaa aggtgttctg
    14461 ctgcccctga tcgtgcttct gtcggtggtg ggcgcctact ccataaacaa ctcggctgac
    14521 gacgtgttgt ggatgatcgc cttcggtgtg ttcggttact tcctcaagct ctacggttat
    14581 cagaccgggc cgctgatcct cggcatcatt ctggccaggc tgattgacga caactggcgc
    14641 cgcgccatca tctcggaacg aggcgacatg accgccttct tcctgaacct cgtctccagc
    14701 ccgctttcgg caatcctgct gggcatcatc gtggctgttc tgctgtcgaa gacgccgttg
    14761 tggcccaagc gagcgcaaac caaggcaaga gcggaggggt cgacatgagc cgggatcggt
    14821 ttctggttgg actgctcggc gcgaacatcg cgaagtcgct gtcgccggcg ctgcatgagg
    14881 acgccttcga cgcccacggc ctgcgcggcc actaccatct gatggatgcc gatcggatcc
    14941 ctttccggcg cctggaacag gccctggccg ccgttaaggg tttgggattc gccggcgtga
    15001 atgtcacgtt cccgttcaag caggcggtcg tgcccttgct ggacgaggtt tcggctgatg
    15061 cgcgccagat cggcgccgtc aacaccgtga ccatcgacgc gagcgggcgc acggccgggt
    15121 tcaatactga ccgcagcggg tttcgccgca gtgtcgaaga ggccttcggt ccggcctttg
    15181 tggcgggcaa gcgggtggtc ctggtcggcg ccggtggcgc ggggcgtgcc gtcgccttcg
    15241 cgctcatgga cctcaaggca tctcatgtgg cgatccacga tgtcgacagc cagcgcgcga
    15301 cggcgacggt cgccgacctg atgacgcatt acggcgccgg gtgctgcagc tatgcggcgc
    15361 atctggcgga cgccatcgcg ggcgccgccg gcgtggtgaa tgcgacgccg atcggcatgc
    15421 tcggcatgcc cggcaacccg gttcccatgg aggccgtgcg ccagcatcat ttcgtcgccg
    15481 acgtcatcta cacgccgatg gagacggcgc ttatcgcggc agcacgccgc aagggctgcc
    15541 aggtgctgac gggcgggggc atgtgcgtgc accaggccgc cgatgcgttt cggttgttca
    15601 ccggcctgga acccgatgtc cgccgcatgg cgacgacctt cgcgcgggcg ctggcgcagc
    15661 gtgatagcgc tttggcgaaa tcggcctagg gcttccggct tgaaaccaag gagcacgcta
    15721 tgaaactctc gatcgccacg gtgtgcctca gtggtgacct cagggtaaag ctcgaagcca
    15781 tcgccgccgc cggcctgcgt ggggtcgaga tcttcgagaa cgacctgctg accttcgacg
    15841 gcaccccttc ggatgtccgg cgcatggcag ccgaccttgg ccttgagatc gtcgccttcc
    15901 aacccttccg cgatttcgag gggttgccgc cggtcaagcg cgagcgggcg ttcgccaggg
    15961 ccgagcgcaa gtttgatctg atggcggaac tcggctgctc gctcatgctg atctgctcca
    16021 atgtcgctcc cgacagtatc ggcggcatcg cgcgcgcagc cgatgatttc aaggcactgg
    16081 gagagcgcgc cgttcggcgt ggctttcgcg tcggttttga agccttgtca tggggacggc
    16141 acatcaacga ctaccgggac tcctgggaag ttgtgcggcg cgccgatcat cccgcgatcg
    16201 gccttgttct cgacagcttc cacatcgccg cgcggcgtac cgacatggcg acgatccgca
    16261 atatcccggg agatcggatc ttcctggtcc agcttgccga tgcgcccatg ctgaacatgg
    16321 actatctcac ctgggggcgc catttccgca gctttcctgg acagggcgaa atgccgctcg
    16381 cggatttcat ggaagcgctc gccgcgaccg gatacgatgg catcctgtcg ctggagattt
    16441 tcaacgacca gtttcgggca ggctccgcca ggccggtggc cgtcgacgga caccggtcgt
    16501 tgatctatgc gctcgatcac ctcgccacca cgaccggccg gcggctgccc ggcctgccgc
    16561 agatgcctcc gagggcgcgc tgcgtcggca ccgagttcat cgagttcgct gtcgacgcgc
    16621 gctccgcgcc gcagcttgag cggctgctcg gcgggctcgg gtttcggcgg acagggcgac
    16681 acaggtcgaa agcggtgtca cgctggcagc aaggggacat caatctggtc gtcaacgcgg
    16741 atgccaacgg ctttgcccat gccttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16861 nnnnntcgaa gctgccggaa gccatggtgc ttgacccggg cggggtcgtg aagagccagg
    16921 tcatccagac ggccggaggc gagctccggt tggttctgaa tgcctcgcag tccgcgcgca
    16981 cgctgtcctc gcgcttcctg agcgaagcct ttggatcggg cgtgcagcac attgcctttg
    17041 cgaccgatga catcttcgcc accgcggcag ccctccgccg ggctggcgtc gcgacgcttg
    17101 caattccgga aaactactac gacgatctcg agacaaagac cgaattgtcg ctcgacgaga
    17161 tcaaccggct caaggcatcg ggcattctgt atgaacagga tgaatgcggc gagtacctcc
    17221 agatctacac gcatgccttt gatgaccgct tcttcttcga gatcgtcgaa cgcaggcgcg
    17281 cctatcaggg gttcggcgcc tccaatgccc agattcgatt ggcggcgcag acccgcattg
    17341 cccgcgatcc cgccataccg aagctctgaa ggctctgacc gcaagcacgc gcaatcgcgg
    17401 cgttcccggc ctcggtccga ccagcgggcg gcgccaaaac tcctgagagg ttagcaatgg
    17461 cccggcgtat ctcattggcc catctgacgg tcctgtcgct ggccccgccg gagatgatcg
    17521 aaacagcgca cgcgacgggc tacgacgcgg tcggcctgcg gctgatcgcc gtgacaccga
    17581 cgacaccagg ttatccgctg atgtccgacc ctgccatgtt gcgcgagacg aaagccgcgc
    17641 ttggacgcac cggaattgcg gtgaacgaca tcgaattcgt acgcatcact ccggagctcg
    17701 acgtcaggtc actcgaaccg ctcctggcca ccggcgcgga cctcggcgcc agacatctca
    17761 tcaccgctcc ctatgatgag gacctcggcc ggctcgcgga caagctcgcc gccattgcgg
    17821 accttgcgca gccctaccgg atcatgccgc tgctcgaatt ctttccatgg gcattcgttc
    17881 cgaacttcgc cggcgcgaaa gacctggtcg cggccacccg tcgggacaat gtcggcattc
    17941 tcttcgatgt cctgcatttt tcgcgcactg gcggctcaat ccagcagctt gtgtcgacgc
    18001 cggcgcggct gttgccgttc gtccatctgt gcgacgcgcc ggccgccatg ccggcgacga
    18061 tggaagcgat gctgcatacg gcccgctgtg accggctgcc gccggggcag ggtgggctcg
    18121 atctcctgcc gatcctctcg gcgctgccgg cggactgtcc cctgggaatc gaagtgccca
    18181 tggaggtcct gaccggtgag atcggcccgc aagcggttgc ccggcgctgc cggcaggcga
    18241 cggatcgtct gcttcagaca tgggcgggca tgtcggcgga tcgcgcctga agcgggaggg
    18301 ctggtcggtg ttcgccgcct cagacaacgc catcgcctgc gatcttctcg tcgtcgggtc
    18361 cggtgcgggc ggattatccg cggcggtcac ggcggcgtgg cacggcctgc gtgtgatcgt
    18421 cgccgagaag gagccggtct ttggcggcac gacggcctgg tccgggggct ggatgtggac
    18481 gccgcgcaat ccactggcgc ggcgcgccgg gttcgtcgag gacatcgacg ccccgcgccg
    18541 ctaccttcga cacgtcctcg gcaatgcgtt tgacgaagcc cgcgtggatg cctttctcga
    18601 cgccgcgccg cacatggtcg ccttcttcga gagggagacg ttgctgcaat tcgaggacgg
    18661 caaccgcatc tgcgatgttt acggaacagt tccgggagcc gggacgggtg gccgctcggt
    18721 catcgcggcg ccctatgacg cgcgacaact cggggcgctg gtggacaagc tgcgccctcc
    18781 gttgcgcgag acggcgttcg ccggcctgcc cattcaggcc ggggccgatc tggcggcctt
    18841 cttgtccgtg acgcgctcgg caaggtcgct ggcccatgtg acaaggcgtg tcggcagaca
    18901 cctgctcgat ctcgcgatcc acggtcgcgc catgcagctg cgcaacggca atgccctggt
    18961 cgcacggctg ctgcgctctg cggcggatct tggcgtcgac ctgcgcgagt cttcgcccgt
    19021 cgtttccctc cggcgcggcg cgcgcgggat cgagggcgca accctatcga ccccgggcgg
    19081 gccggttcat gtcagcgccg cacggggcat cgtgctggcc gccgggggat ttccccatga
    19141 tgtcgcgcgc cgcggcgcgg tcttcccggc ggcagaccag cattggacgc tggctgttcc
    19201 gtccgcgacg ggcgatggcc tgcgccttgg agaggcggcc ggcggcatcg tcgacacctc
    19261 ccttgccgca cccggcgcct ggtgtcctgt gtccctcgtg ccgcacccgg atggaaccac
    19321 gggacgattt ccccacatca tcgagcgcgg caagcctggg atcatcgccg tgttggcgga
    19381 tggcaggcgg ttctgcaacg aggccaacgg ctaccatgac tacgtggagg cgatgttgcg
    19441 ctccgtacca cgcgggctgg agatggcctc ctggttgatc tgcacgcgcg acttccagcg
    19501 ccggtacggg cttggcatcg cgcgacccag ccccgttccc gaaggaccat acatccgctc
    19561 cggttacatc aagaccggca ggaccctcgc cgacctcgcg cgcgcttgcg gaatcgatcc
    19621 cggcggtctg gagcgcagcg tcgcaaccta caatgagcac gcccgcaggg gcgaggatcc
    19681 gcaattcggc cgcggcgcat caccctataa tcgcctgcag ggcgatgcga cctgccggcc
    19741 caatccctgc gtcgcgccga tcgagaaacc accgttctac gccgtcaagg tggttcctgg
    19801 cagcttcggg acctttgccg ggctgaagac cgatgcccac gcccgtgttc ttgaccgcgc
    19861 cggcgtgccc attgccggtc tctatgcgtc cggtaccgac atggccagca tcatgggtgg
    19921 ctactatcct gccggtggca tcaatctcgg ccccgccatg acgttcggct atatcgccgg
    19981 ccgccatgcg gcgggcaagg acgctgtcgg gttcgaaacc ggatgagcgc gccttgtcgg
    20041 ggcctgcctg cccgggggca aggtcggtgg cccgcccttt ccgttcagtc cggcaaccga
    20101 tggtttacaa aggcgggagg tcggcttatg aggcgcagcg ctgttcgggc accgtgcacc
    20161 acgcgatggt tgtccgggca gcatctggag aggtggccga gtggttgaag gcgcacgcct
    20221 ggaacgcgtg tatacgggaa accgtatcga gggttcgaat ccctctctct ccgccagtca
    20281 tccccacaca ttgtcgcctt gattgatctg atccggcggc cgctggcagc gtgaggccat
    20341 caagggtcgg ttctatttgg caccacgccg ttgagcgagg gtcccgccgt ttgcgcgtgg
    20401 ctcgaaggac gtgttcgacc gcgccgagat tccctgagag ccaaccggcg aacacgcttg
    20461 gaacgggatg ccgtcatcga tcgagctcaa gtgcctgccc aaaccgaaag ccgaaggcgt
    20521 ctggaaaact cggacggttc ggtcgagtat gaaatcatgc ctcgtgacat gacttgaccc
    20581 atcaagccgt gaaccttcca cggcatgagg tgatccccac ctacgcacga gtcagcgacc
    20641 ggcgttgtac tgaagcattg actcttcaaa acgatttgga tcatatccaa aacgttttgg
    20701 ggaaacgctt atggcaacgc tgcgaagcct tgcgtccgat cttggcttgt cgatcacgac
    20761 cgtttcgcgc gcgctcgacg gctatgagga tgttgctgcg gcaacccgcg agcgcgtgaa
    20821 caaggccgcc gccgccgccg gataccgtcc caactccact gcccggcgat tgcgcaaggg
    20881 atcaagcgag acggtcgcgc tggtcatgcc aaccgagccg gggcgtttct atgaacctgt
    20941 gtttgtggat cttctttccg tcctcggaga gcggctggcg gcccgccact tcgatctgat
    21001 gctgctcgcc gcgcgtccag gcgcggagga actggcggtc tacaagcgca tgatcaagga
    21061 caggcgcgcc gacgcctgca tcatcgtgcg cacgcgccgc catgacgagc gcatcgcgat
    21121 gctgcaggag gctggactgc ctttcgtctg ccacggccgg accgagacgc gcgccgagta
    21181 tgcctatgtg gatggtgacg gcgaggctgg cttccgggag gtcacgaacc gaattgtgtc
    21241 gctcgggcac aggcggattg cgcatctggc ggcgcccgac cacttcactt ttgccgaatt
    21301 gcgttcccgt ggctggcaca tggcgatgcg cgcggccgga cttaccgatg acctcaagct
    21361 catgtgcgcg tccactgaag aagctggcga gcaggctgcc accgcattgc ttgccggacc
    21421 ggacaagccg acagcgctga tctgcgcgac cgaccgcatc gccattggcg cggtgcgggc
    21481 ggcgcaggct gccgggcttg tggtgggtcg cgacatcgcg gtgacgggtc acgacaacat
    21541 ccacgcttct cggttcaccc atcccgccat caccacgatg gaactcgatg tgcggtccgt
    21601 cggcgcgtgt cttgccgaca agctgctgca tctcgtcgag cgcggcatgc cggagcacgc
    21661 cggcgacgtg ttccccctac ggcaggtcct gcgtgcatcc tccggcgagg caaactgacg
    21721 ttcaaccgaa ccagcggcgg ggaaggcccg gcgcttcgaa cccaagggag accacaatga
    21781 ctggattgaa gcgactgctg gccgcgaccg ccctgacagg cgcggccctc tccggcgcca
    21841 tggcgcagga aacgattttc ctctcgaccc agctccgccc tgtcgaagag gcgaccaagg
    21901 tgcgtcagat cattctgaaa ggcgctccag ggccggtgac ctttgtggtc gaggagccct
    21961 cacctttcca gacgcgcatg gacgcggaaa cgcaggccgg caagcggacc atctcgatgg
    22021 tgggcgcact ccatggcgaa ctcgcgccgc tcgtcgggtc gaagggactg gaaccgctgg
    22081 atgcccttgt cgccaaggcc aaggaacgcg gtgtgcccga tgcgatgctg gcgctgggta
    22141 agctcggcac ggacaagcag atgtacatcc cgtggatgca ggcgacctac atcatggccg
    22201 ccaagaagga agcccttcag tatctgcccg ccggcgccga tgtgaacgcg ctgacctatg
    22261 tccagctcaa ggactgggca aagaacatcc aggacaagac cggcaagcgc gcgctcggat
    22321 tccccgccgg ccccaaggga ctgctcgcgc gcttcttcca gggctatctt tacccgtctt
    22381 ataccggcgg cgtcgtcgcc tcgttcaaga gtgcggaagc cgaggcgatg tggggtgcgt
    22441 tcaaggatct ctgggcgcac gtcaatccca actcggcgtc ttacgacttc atgcaggagc
    22501 cgctgatggc tggcgaggtg atgatcgcct tcgaccatgt tgcccgcctc aaagatgccc
    22561 tggtcgccgc gccgaacgac tatgtcacct tccctgcgcc ttccgccgcc aaggggcgcg
    22621 gcttcatgcc ggtcgtcgcg ggcctcggaa ttgcgccaaa cgccccgaac aaggcagggg
    22681 cgatggccgc catcgaacat ctcctcaagc cggaaacgca ggtgacgatg gcgcgcgaac
    22741 tcggcttctt cccggtggtg aaggccgact tgccgaccga tctgcctggc ggcatcaagc
    22801 ttctcgtcga aggcgttgcc aagacgcaag gggcgaagga tgcgctggtc tccctgctgc
    22861 cggtcggcct cggcgccaag ggcggcgagt tcaacaaggt ctatgttgat accttccagg
    22921 ccatcgtgct gcgcggtgcg ccgattcgcg cgacacttga tgcgcaggcc gcgacgctcc
    22981 agaccatcat gaacgagacg aaggcgccgt gctgggcgcc ggacaagccg agtgacggag
    23041 cctgcaaggt caactgagag cccgcggtgc attggccctc tcccgtgcgg gagagggcgc
    23101 ttccggtgag gtttccatgt cgcgcacgcg cctgctccca tatctgttaa tcggcccggc
    23161 gacggtattt ctgctggtgc ttttcgtcgt gccgttggtg cagacgatcg ctttggcctt
    23221 cacggacaac ggaacggcct cgctcaccaa cttctccaag atggcgggcg acctcaactt
    23281 cggcctgtcg ctgcgcaaca ccttcctgct ggtgctggcc gtcatccctt tgcagctttg
    23341 tctcgcgctg atgatggcga tgatggtgca gaaactggag aaagggcgcg acctcgtgct
    23401 ctggatcctg attattccgc tggggatctc cgatttggcg gcgggcctcg tatggctctc
    23461 cattcttcag gacaggggct atttcaatac cgtcctaaac atgctgggtc tcatcgacgg
    23521 gccagtgaac tggctgtcct atgaggcccc cctcagcctc ttcatcggcg tcgttctggc
    23581 ggaagtttgg cgtgccaccg cgatcgtcct ggtaatcctg atcgccgggc tgcagctgat
    23641 tccgaaggaa tacgccgaag ctgcggaaat cttcggcgca acagcctgga cacggttccg
    23701 caagattacg ctgcccctgc tcaaaccctc gatccagtcg gcgctcatcc tccggacggt
    23761 gcttgccttc gaggtctttg cggtcgtcta tgcgctttcc gggcgcaatt tccccgtgct
    23821 ggtgggcgag gcctatgcct ggcagcatgt gaaccagaac aatggcgttg ctgcggccta
    23881 cgccattttg gtgatgggga tctcgcttgc ggcgacggtc atctacatca aggcgctgag
    23941 gatcaggccg gagatgcagc catgagtgcg gcgagcgttt cgcccggatt gcgccggctt
    24001 gtcttctggt ccggcgtcgc gatgctctgc gcttgggtgc tggtgccgat ctacctgatc
    24061 gcgcttggcg cctttggggg acgcctgggc gtgtttcgct ggcccaagaa cctgctgccg
    24121 tgggatacgt ctcttgctgc gatgcagacc tttctggcag tggagggcgt ctgggcggct
    24181 gcgtggaacg gtgctctggt tgccggcctt accatgctgt tctcgctgct gctcggcgcg
    24241 ccggcaggct atgcgctggc gcgttactcc ttcgccgggc agaacgcctt ccgcatgctg
    24301 gtggtactca cccgtgcctt tcctctcgca atccttgctc tgcccctgac ggtgacgttt
    24361 atccgggccg gcctgtacga cacggccatc ggcgtcgcgc ttgttcacac ggcgttggct
    24421 ctgccctttg ccgcgctggt ctcctccagc ctgttcatgg gcatcccgag ggaactggag
    24481 gaagccgcgt gggtattcgg gtgcaccggc tttcaggcct tccgcaaggt cgtgctgccc
    24541 ctggcgttac ccggtctggc ggcggccgca atctttgcct tcgtgatatc gtggaatgag
    24601 gtgttcgcgg cctcggtgct caccgtgcgg aacaagaccc tgaccgccta tctactgacc
    24661 gttttggcgg aatcgccgct gcactttcgc ttcgcagggg gtttcctgct catacttccg
    24721 tccgtcatct tcatcttcgc cgtgaggaaa tacctctttg cgatgtgggg cattgccaac
    24781 agatagggtc cgttatggct ggcatcacaa tcgaccggat tgagaagaag ttcggaacgg
    24841 tcaccgcgct gcagcaacta tcgctggagg tcgcggacag cgagttcctt gccctgcttg
    24901 gcccttctgg atgcggcaag accacgctgc tgcgcatcat cgccgggctg gaaagccaga
    24961 cctccggccg catccttatt ggcgggcggg acgtctccgc actgccgccg cgccagcgcg
    25021 gtctcgccat ggtgttccag aactacgccg ttttcccgca catgacggtg tacgagaacg
    25081 tggccttcgg tctgcgcatg aagggggcag gcgatgccga ggtgaagaaa caggtggagc
    25141 gcgctgcggg cctcttgcat attgagccct atctggagcg ctaccctgca aagctctcgg
    25201 gcgggcagcg tcagcgcgtg gcagtggcgc gcgcattggc cgtgcaaccg gctgtcctgt
    25261 tgatggacga gccgctgtcg aatctggatg cgctgctgcg tcttgaaatg cgctctgagc
    25321 tcaaggcggt tctttccgct tcaggcacga ccacggtcta tgtcactcat gaccagaccg
    25381 aggcgatggg gctcgccgac cggattgccg tgatgcacaa gggcgttatc gagcagatcg
    25441 acaggccgca gacgctctac gagcggccgg cgacgcggtt tgtcgggggc ttcgtcggct
    25501 ccccgccgat gaacttcatc gatcttgccg tgacagctgg caaagccagc ctcggcgcct
    25561 tttccagctc ggttgcctta agcgagggca ccatcaccgc gggcatccgc gccgaggaca
    25621 tcacggtatc cgacggcggc ggcatgcctt tcgaagtcag cgtctgcgaa cccttggggg
    25681 cgaccctgct gctgaccggc cgcatcgagg gacaactggc ccgcgtctcc gtgcgcccgg
    25741 atgcacgcgt caggcccggg gacaggttgg ggctcgcgcc cgccctggac cgcatagtct
    25801 ggattgatcc gcacaacggc aaggctttgg gagcagcgac gcagtcgtca tgaagatgtt
    25861 caacgatacg aacgcccagg ccaaagccat tctcgcgggc aatgaccggg gtggctacac
    25921 ggtgccgacc catgggctct acccgttcca gtggaactgg gattcggcct ttgtcgccat
    25981 ggggtttgcg acctacgata tcgaccgcgc cttgcgcgaa ctggagcgcc ttgccgaagg
    26041 ccagtgggac gacgggatga tcccccagat cgtctttcac gctccggcag acagctattt
    26101 cccgggaccg gaagtgtggg gtacgcacca ccgcatcgcc acctccggca tcacgcagcc
    26161 gccggctttc gcgatggcgc tgaagttcat tgccgatcgg gccggcgccg cgttcgaacc
    26221 gcggatccgg gcgttgtttg cggtggcgga tcggtatcat cgctggttcg ccacagcgcg
    26281 cgatccggat ggaaccgggc tggtctcgac gctgcacaac tgggagaccg gtcgcgacaa
    26341 ctcgcctgaa tgggacgtgc cgttcgcgcg tgtccctgaa acgaccgcca ccatcatccg
    26401 tcgtcgcgat accgggcatg tcgatgcctc gatgcgtccg acggatgctg actaccggcg
    26461 ctacatccac cttgtcgacc tctaccgttc gctcgactgg agcccggcag caatgctgga
    26521 ggcagcgccg ttccgcgtcg cggacatcgg caccaacgcg attctgctgg cggccgacga
    26581 ggccctgctg gaacttgcgc cccgctttgg cgcgagcggg tcggagttgg atatccgcgc
    26641 ccggatttcg cgtttgtcgc atggcctcgc gaggctctgg aatgcggagc gcggctggta
    26701 tctttcgcaa gaccttctcg cgatggagcc aatcgcggtc cggacgtcgg ccgggctgct
    26761 ggccctgctg acgactgtgc ccggtcgtgc gcaggtggat cagatggcca gcgccgtcgc
    26821 ggcccatctt gatgcggtca ccatgggcat cgcctccacc ctgcccggag agccgggctt
    26881 cgaacccaag cgctactggc gcggtccggt ctgggccatc gtcaactgga tgatcgcgga
    26941 aggattggcg cgccatggcc atgcagcgct cgccgaaagg cttcgcgcaa tgaccgtctc
    27001 gaccatcgaa actcgcggct ttggagagta tttcgatccc acgaccggcg atgcgttggg
    27061 aggcggcgcg ttttcatgga ccgcggcgat cctcctgatg ctgaaggctg cgtgagtcac
    27121 ttgcccatgg atcatgtgat gatgcgcgag gccttgaccg aaggtgagct acccccggag
    27181 tgttcctcgc tctgatgtaa agactgccag cctgaggagg agcaggcaac atgaggaaga
    27241 gccgtttcac cgatgcgcag atcatcggga tgaaaaagga acgggaggcg aggctgctcc
    27301 tccagcacag gcgaaatccg cacaccgctg cgtgcctggt gcgagaaaaa gacggcgccg
    27361 tcagttgccc cagcgctcgc gatacatgcg ctcgccaatc atgcaatcgg acttgcggcg
    27421 gtcgttctgg gcatactgta catcgcgcgc accgaggtca gcgatttccc gcaggatttt
    27481 cgagcgtgtt gccggcacga actggtcgcg cgagacgatc gccaccatga acgcgcccgg
    27541 cccgccgatg acgcaatcct gatagtagag gtcgagatcc ttgatgtcgc cccagccata
    27601 gccggtctgg cgtttcagca cgatcggcag tccgttgatt acaatgccgg ccgacaacgc
    27661 ccggtcccgc gcaacctcca ccggatcgcc attattgttc ggtccgtcgc cggagacgtc
    27721 cagcaccttg cggaaggcac ggtagggcat ggcctcgatc aacttgtggc cataggcgat
    27781 ggcgccggag acagaagttc tctgagaacg ccgcaacggc gaggcaacaa tccggtccgt
    27841 taccgccctg gcgctttccg ggccatcgat gatcgtccag ggtacgacca tgcgctggtc
    27901 gaaggcgttg gcccattcga aataggacac cgcaatctta cccgtcatgc cggagcggat
    27961 ggcctgcagc acctcgggcg aattcagcgc ttccacgtag ccttgcttct gcagggcttg
    28021 ttcctcaggg tccatggaat aggagatgtc gaccgcgaaa acgagggcga catccacctc
    28081 ctcgccctca gccgcaattc cccttgttgc cgataggccc aaggccagca acccggcggc
    28141 acagagtgtc gccaaccgtg ccgcaatccc ccctctggta ccgccatcca tgagtcgcgc
    28201 tcctgagatt gcgttttggg gaagactgcg ctctgccggg caggattgtc cagaaaaaac
    28261 tccagggctg ccatcggaac agatcacagg cgcattaccg cgtgcggcgc caatcccctg
    28321 gcttgcggat catcgtcccg tcgggccgtg ccagctatcg aggcgcgtac cggctgccga
    28381 gcaggggcgg cccatcgctc acgacggctc cccgttcgac cagatcttcc agatgggcca
    28441 gcaccgagag cccggcagcc cccttcaggc gcggatcgag cccctcgtag atcgaatcca
    28501 caagagcggg aatcgtctcg tcgccggccg cgagacgttt caggatcgat gtctcgcgca
    28561 tccgccggtg gtggatgagg gcacgcacaa agcgaccggg ttccgtcacc ggtccaccgt
    28621 gacccggcca gtaaatcgtc tcgtcacggc ctctgagccg ctccagcgac agcatgaact
    28681 cccgcatgga tccgtccgga ggggcgacga tcgatgtcga ccaggccatc acgtgatcgc
    28741 ccgagaacag ggcattttct tccttcaggg cgaaggcgag atggttggcg gtgtggcccg
    28801 gcgtgaacac cgcctcgagg gtgaacccat cgcccgcgag cacttcgcca tccgacagcg
    28861 ccacgtcggg ccgatatgcg gtgtcggcgc cggcttccat ggcgatctcg cccacatgca
    28921 agtcgcgaaa ggccatgtgg ggcttgcagc cgacgaccgg tgcgccggtc cgcgccttca
    28981 gcagtgcggc gccgggtgaa tgatcgcgat gggtgtgggt gacgacgatc tgtgcgacgc
    29041 gttcatcacc cagagcctcg agcagcgcgg cgatatggtg cgtgtcggct gggcccgggt
    29101 cgatgaccgc gacgtttccc tggccgacga tgtaagtgca ggtcccggtg aaggtgaagg
    29161 gtccgggatt tgcgaccgga agccggcgga caagcgggga aagctgatcg aggcgacccg
    29221 gaacgggggc ttcccggttg aaggccggtg tctcgggtgc ggcctcgccc aacggggcga
    29281 gaggttcgat cccggtcatg tcaaggcgca cgagggcgct cccgataggt tgtcccgcga
    29341 gggagaggtc aggcgcgacc tccgccaggg gtgccagcac gaaggcgcgc tcgagcgcgt
    29401 gccgatgcgg caaggtcagg tgcgcgttct caagctgcac ctgatcatag gcgatgatgt
    29461 cgatgtcgat gacgcgcggt ccccactttt cgcggcgctc gcgtcccagg cgcttctcga
    29521 tggcaagaca ggcgtcgagc agggcgtggg gcgtcaggtc cgtctcaacc gcgacggcgg
    29581 cattgacgaa ccagtcctgg tcggtcttgc cccacggcgg cgttcgccag tcgcgggagc
    29641 gggcaacgac ccgggtctga ggcagtgcgc cgatcaggtc gatggcgcgg gcgatcgttc
    29701 cgcgtttgtc ggcgatgttg gcgccgaggc ccaaccacgc ggcatgctgg aagaccttgg
    29761 acacgggtca ggatgtccgc tgacgcgtga cttcgaccga gacgttctcg atcatggccg
    29821 gtattggcgc agcaggcttt tccactgtca cggtcacctc ccgcacgacg ggaaatccgg
    29881 acagcaccat cgccgcgacg gcttctgcca gggcctcgat ggttttgaac tggcgaccga
    29941 ccgcgacctc ctgcgtctgt tccgtcatcg cgtggtagtc ggcggtcttg gtaaaatcgt
    30001 cggcgatgcc ggccggccgc agatcaagcc ggcagtcgat cgagatgaag aagcgttgcc
    30061 ctagccgggt ctcttccggg tgcagcccgt ggtaggcgaa gacgccgatt cgggtcaggc
    30121 gtatacggtc ggtcatggtg tcccctggtt cggcgtgacc gaaccccgga tgatggcgtc
    30181 ggtcaaggcc agtgcctcgc gatgcggccg gatgtcgtgc gcgcggatga tcgacgcgcc
    30241 cgcgagcgcg gcggcaacat gggcggcgag ggttcccggc aggcgttcgg cgggaacaga
    30301 ccgtcccgtg actgcgccga taaacccctt tcgggaactg ccgaccagca gcggcaggcc
    30361 aaaggccttc agttcggcga ggcgtcttag gacgatcagg ttctggtcga atgtcttccc
    30421 gaagccgatg ccgggatcaa gcacgatgcg tgcctgggga atgccggcgc gtgccgccat
    30481 gtcgagggac cgctcaaaga acgcgcggat gtcgaccagg atgtcgagac tgggatcgac
    30541 cgtcgtccgg ttgtgcatga tgcacagccc ggcgccgtga tgcgccacca cgcgcgccat
    30601 ctctccatcc cgttgcaggc cccacacgtc gttgacgatg cgggcgccag cggcaagcgc
    30661 ggcgtctgcc gtgcgcgcct tgtaggtgtc tatggaaacc ggcagcgcga ctttgtcagt
    30721 gatggcgcgc agtcccggca ggacacgggc cagttctgtc tcggcatcga tgggcgtggc
    30781 tctgggatgg gtggattccc cgccgatgtc gagaatatcc gcaccttcat ccgccagacg
    30841 catcgcggtc gcgaccgcct ggtcgacacc ggcaacatgg ccgccatcgg aaaaggaatc
    30901 cggcgtcagg ttgatgaccc ccatgatacg cgtgcgcccg ccggccgcct gcggccaggg
    30961 attgtcattc ggggtgtttg gctggaaagc catgggcagt gcggatttgg tctggcacga
    31021 gggagaagcc cgtctaagtc ctgaatgcag ccatgccaag caaaacctct ccaagccgcc
    31081 gcgcgctgcg ggcggctttt cctgtggcgc gggttgcgct agtccgatgg ccatggatca
    31141 taccgattgc attgtcattg gcgccggagt cattggactg tcgatcgcgc gcgaactagc
    31201 gctggccggg cgcgaggtga ttgtgctcga agccgagaaa gtcatcggca gtggggtgtc
    31261 cgcccgcaac agcggtgtca ttcacgccgg attgtactat ccggctggcc tcgcgaaagc
    31321 ccgactgtgc gtcgagggca agcggcggct gtatgatttc gcccagcgtt gtggcctttc
    31381 gcacagccgt tgcgggaagc tcgtcgtggc cacggcgccg gaacagaatg cgcgccttga
    31441 ggcgttgaag cggcagggcg aggcgaacgg cgtcgacgat ctcaagatgc tgacagcctc
    31501 cgaggcacgc acgctggagc cggaactggc ctgcgtggcc gccctgctgt cgccatcgac
    31561 aggcatcgtc gacgtgcacg actacctcct cgccctgcgt ggcgaggcgg aagcgcacgg
    31621 cgcgatgatt gcgttcggaa ccccggccct gtccggctcc gcggaggacg acggcatccg
    31681 catcgagacc ggcggggatt cgccgatgac gctcaagggc aaagctgtaa ttgtggccgc
    31741 agggctgcag gggcagtctg ttgcgcgggc catcgtcggg atgccggcgg aacgggtgcc
    31801 gccgctgcat cttgccaagg gcaactacta cagcctgacc ggccgccaac ctttccgaag
    31861 gctgatctat ccagttccgg aagatggcgg ccttggggtg catgtgacca tcgacatggg
    31921 cggacagacg cgcttcggtc ccgacgtcga gtggatcgat accatcgact attcggtcga
    31981 cccgtgccgc gccgatgcct tctatgccgc catccgcaga tattggccgg cccttcccga
    32041 tggcgctctt gtacccgcct atgccggcat ccgtcccaag atcgaaaggc ccggtggctc
    32101 gaccacggac ttccttgtcc tgaccgggaa agaacacggt gttccggggc tggtgactct
    32161 gttcggcatc gaatctcccg ggctgacggc ctcgctcgcc atcgcggcag aggttgcggc
    32221 ggcgatcaac ccatgaggat ggatacgatg gcggacatcg tcgcctacgg ggaaccgatg
    32281 ctggagttca accacaccgg agaggggggc gagcggctct acctgcaggg atttggcggc
    32341 gacacgtcca acttcgtcat cgctgcggcg cggcagggtg cgcgcgccgc ctatgtcacg
    32401 gcgctcggcg acgaccgcta cggggcgatg ttccgcgcgc tctgggctga agagggcgtg
    32461 gatgcgtccg gcgtcagaac cgatccggac gcccctacgg cggtctactt tgtcgatcat
    32521 gacgcaagcg gccaccattt ctcctttttc cgcaaggggt ctgcggccag ccggttccgg
    32581 cccacggacc tgccgcgcgc tctgatcggg caggcgcgat tctttcacct ttccggcatt
    32641 tccctggcga tttcggatac ggcttgcgat gcggggctgg aagccatgca tctggcccgg
    32701 caggccggcg cgacggtgac gttcgacacc aacctgcgcc tgcgcctgtg gccgaagcat
    32761 cgggcacgcg ccatcatcac tgaagccatg agattggcgc atgtctgcct gccgagcctc
    32821 gacgacatca ttgccatcgg tggacccgag cagccggacg ccatcattga tcatatcctc
    32881 gatcttggcc cgcgcaccgt tgccctgaaa cttggcgcgc gcggggccat cattgccgat
    32941 gccggcaaac gcgcgatgct cccgccgcat ccctgccggc cggtggatgc caccggcgcg
    33001 ggcgattgct ttggcggcgc gtttgtggcg cgccttgccg caggagacga tccggtcagc
    33061 gccggtcgct acgccggggt tgcggctgcg ctgtcgaccg aaggctatgg cgcggtcgcg
    33121 ccgattcccc gcgcgtcgac cgtgcgagcc gcgatggccg gcgcctgaat gacagcagcg
    33181 tgtctcgcag gtcccttggg cgttcctcga acagcctgcc agcgagcttg cggcaggtct
    33241 tgagccgctc ctcgagcttg cgatcaatgc gcttgagcaa gcggtgcagc gcctcgcctt
    33301 cgccgttgcc ggccagttcg gtgtgcagca gcgacagatc gcgatgctcg ccgatggcaa
    33361 cctgcaaccg cctcaggcga ttggagcgcc gatcgagacg gtgcgaagcc ggctcgccga
    33421 gccaggcggc caggaacgcc aactgatggc ggatgtcatt tacggacttg cgccattcat
    33481 ggaactccgt gaggtccgga tcagcaaggt cggatggcat gcgtcgccgt gcacgccggt
    33541 acccttgggc cacggccgcg agaattgcgc catcggcagc ggatcgtggc ttccatccag
    33601 agatatgagt gcgaatccgc tgaagaacct cgactgctga tccgatcgat tgcaccgctt
    33661 cgtggttggc ggctaggcgc cgctcgcgcc acgtctcgac aaagcgcgag gctttcccat
    33721 tcagtccacc acccttcggt gcgatccgct cgaaggattc ggccagcgca tcgagatcgc
    33781 gggaaggacc cagcgaccgc cgaacggacg caagcaccat gcgcgccgcg cgagccttgc
    33841 ggccgagacc agggggcgcg agcctgagca gcgcgctggt cgccttggcc gcccgtcgtc
    33901 cctgctgcac gcgtcgaggc tgaggcagtt cgggatcggt caggtcagca atccctgcgg
    33961 ccagaatctc cagcagccgt atccgccaga aaaaggcgag cgcgggggtg tggcccacag
    34021 ctccggagcg cgcttcgtcc ggcacgtata cctcttcagg attggccatg gatgtggccc
    34081 atggcgctga ttgaacgtgg gttcgacaga ggatgccact tcctgctgat cagaagggtc
    34141 acgacgagac aaggccaagg tatcgcgcct tgccatgggg cgcagccgca atctgaccgc
    34201 ccttggagag tggggcactc atcgatgacg gacatatgac aggaggcgcg aatttggcgt
    34261 tgacgccgtc gcgacctcgc atgcagggtg caacccgacc taacgtcttc agccagattc
    34321 aatgtggagc agcccatgcg tttcctctcc ttctcggtcg acggcatgcc gtcctacggc
    34381 atcgccactt cgaagggcgt gcatgacctt ggcctgcgcc acggcaatgt gtttcccgat
    34441 ctgcgcagtt tcctcgaagc ccagtcgacc ggcctgctgg cgcccctgtc cgtgccgagg
    34501 gagacggact acaagctgtc cgagatcgag tatgatccgg tcattcccaa cccgggcaag
    34561 attatctgcg tcggcgcgaa ttacgtcgcc catcgcgagg aaatggggcg gccggaaatc
    34621 ggccatccca ccttgttcac gcgctatgcc gacacgctgg tcggccacaa ggccggcctg
    34681 gtgaagccga acgcgaccga tcgcttcgac tatgagggcg aattggccgt gatcatcggc
    34741 cagcattgcc ggaacgtgcc ggactccgag gccatggacg tcattgccgg cgtctgttgc
    34801 ttcaacgacg gctccgtgcg cgattggcag cgccactcct cgcagttcac ggcgggcaag
    34861 aatttccccg ggactggcgc tctcggaccg gagatggtga cgctggacga actcggcgat
    34921 ctcggtcggc tgaaactcga aaccaggctg aacggccaga ccgtgcagtc cacgtcgctg
    34981 gacatgatga ttttctcgat caccgccagc attgcttaca tcaccgcgtt cacacccctg
    35041 gcgccgggcg acatcatcgc cacgggcacg ccgggcggcg tcggcgacaa gcgcaatcct
    35101 cccctttaca tgaaggtcgg cgacaaggtg gaagtggtga tcgagaaggt tggccacctg
    35161 accaacccgg tcgtcgccga ggacgaagcc tgacggcgaa aggagccgaa cggccatggt
    35221 taccatctac cgcgcacgca agatcattac catgaacccg gcgcgtcccg tcgccacgca
    35281 tgtcgccgtg agggatggcc gggtgctcgg agcgggaagc tacgaagagc tgaagggctg
    35341 gggagaacat cgtctcgatg agcggtttgc gaccttggtc ctgatgcctg gcatggtgga
    35401 agggcatagc cacctgtccg agggcgtact ctggcgccat gcctattgcg gctacttcga
    35461 ccgcatggat ccagacggga aaacctggac gggcgcgcgt tcgatcgagg agatcgtggc
    35521 gcggcttcgc gatgctgagg ccggcatggc cgatcctcag aggcccgtcg ccggctgggg
    35581 tctcgacccg atctattacg gcaaccggcg cgtcatgcgt gccgatctcg atcaggtctc
    35641 gacccggcgt cccgtcggcg tcgtgcatgc cagcggccac atcctgaacg tgaacacgcg
    35701 tgggctggaa ttggcgggtc tgatgcgggc gggcatcaat caccccggcg tcgtcttggg
    35761 ggccgatggg ttgccgacgg gagaattgaa gggaccggaa gcgatgtcgc tcgcggcacg
    35821 gcacatcggc atggatcgcg gacttctcaa ttcagacgag gcaggccttc gcgccttcgc
    35881 gcggctttgc gtgcgcaccg gcgtcaccac tgcggcggac ctcggcaaca tgctcgagcc
    35941 gcaagccgtg gagatgatga cgcgcgtgac cgcggaagag cgctttccgg cccggatcgt
    36001 ggccctgcgc atggcattcg acatcgagcc ggaagccctg atcgccaggg cgcgcgaagt
    36061 ctcggggatg agcacggatc gattgaggct cggaatcatc aaggttgttg tagatgggtc
    36121 gatccaggga ttttccgcac gcctgctctg gcccggctac tacaacggcg caccgaacgg
    36181 cctgtggtat atcgcgccgc agcgtctagc cgagattttt gagcgggcgc tcgcggcagg
    36241 tatccaggtc cacatgcaca ccaacggcga cgaagccacc gaactggtac tggacaccat
    36301 ggaagccgcc ttgcgcaagc accccagtgc cgaccaccgc ttcaccctgc agcattgcca
    36361 gctggcgaac cgcgcgcagt tccggcgcat gaaggcgctg gggatgaacg tcaacctgtt
    36421 cccgaaccac catttctact ggggcgacga gcattatgcg ctgacggtcg ggccggagcg
    36481 cgccgagcgg atgaacgcct gcgcctcggc acttgaggcc ggcgtgccct tcgccatcca
    36541 ttccgacgcg ccggtcacgc cgctcggccc gctgttcacc gcatggtgcg ccgtcaaccg
    36601 ccgcacggcg tcaggccgga ccttgggcga agccgagaag atcccggttg gcgaggccct
    36661 gcgcacgatc acgctcggag cggcgcatac cttcaagctc gatgcggaga tcggctcgat
    36721 cgagaccggc aagcgtgcgg atttcgccgt tctcgaggac gaccccgaac atgttggggc
    36781 cgagctgctg aaggatgtgc gggtttgggg cacggtgctg ggcggccggg tgtttgccgc
    36841 cgccgatata tgaccgggcg cattccggtc acggtcattg gcgggtacct cggcgccggc
    36901 aagacgacgc tggtcaacca tctgctgcgc catgccggcg gcctgcgcct cgccgtgatg
    36961 gtcaacgact tcggcgccat ggccatcgat gccgacctca tcgaggcgca ggaagaggac
    37021 ctgatctcca tcgctggcgg gtgcatctgc tgcagcttcg gcagcgacct gatcgaagcc
    37081 atggcgaagc tcgccgagcg cagtccgcgt ccggatcatg tgctcctcga gacaagcggc
    37141 gtggcccttc cggggccggt ggcgggaacc gtttcgctcc tgccggattt cgatgtcgac
    37201 tgcgttgccg tactggctga cgcgacacgg attcgggctc tcgtggctga ccgctatctt
    37261 gcagacacca tggaccgcca gttggcggat gcgcagatcg tgcttctgac gaagaccgat
    37321 tgcatcgggc cgtcactggc cgatgagacg ctggcgtggc tttcaggacg cgtgccaggc
    37381 gcgcgctgcc tgcccgtcag caacggggcc gtcgatccgg ccgtactgat cggcgcgcga
    37441 ctggggtaca gggcaacgtt gtcgctcagt gcaccgcatc aggcggccat gctctaccat
    37501 accgaaagtt tgccgatggt ggagccactg gacatcgcag cgctgacgac gctcttgcag
    37561 ggggccgatc tcggcctgct gcgcgccaag ggcttcgttc gcaacgcagt gggacagatc
    37621 tatggcgttc aggtcgttgg aaccgtggtt cgcgtcgagc gccacctcgc gccgaggcct
    37681 gtcgagccgg gattgatcgc catcgggctg cgccggagct tcgatcctgc cctctttcgc
    37741 caggccttgg cggtgacgcg ggtcaccggc gattgaccag ggcggcgacg gccagcgcaa
    37801 tccagccgga aatcgtcaac aaaccgccga tcggtgccgc attggcgaac agccgcatgc
    37861 catgcaaggc gcgcagggtc aggtctccgg caaacagtgc gacccctaag gccatcacaa
    37921 cgagcgccgg cagggcgagg ccggcccgca gtcgtcctgt cgccagtgcc agggcgccgg
    37981 ccatcagcgc cggagcgtga aacagcagca tctgcccggc ggtcgtcgcc tggttgcctg
    38041 cggcatgcgc accggcggcc aacaaggcta ctcccgaggc gccgaacatc gacgcgatca
    38101 gcagcagcac gcgcacggac atggcgaaac cctagtggtt ggacgcttat cttagacgac
    38161 gtccgagcct ctggcgatcc tggggcgtct ggcttgcgct cgaagccgga ctttcgaggc
    38221 ttccgccgat caggtatccg atgctggctg tgagtttgtc ggcgagttcc ggctgttcga
    38281 cgtagcgccg cgccagggct cgtgcgatct gcggcgcggc gacgctggtt ccatccatgc
    38341 ggatgaccgc gccgctgcgt gttccgtgcc cgggcacgcc gcgcagcacc ggattgctgt
    38401 cggaaggtgc tgagaacggg aggccgggcg acgcgaacga aggaccggag gcataaacac
    38461 tcggcttgcg cgtctgccct acaaatccac caaccacggt cacccggtcg tgggtcgcca
    38521 gaccgttcag ggttccaaag cgccgaacaa acgccgccgg cgggttttct gcatcggccg
    38581 gtgcaccatt ggcgcgcagc accccatcgt acgggtcgtc aagatagctc tgacgcgcgc
    38641 cgcgctgata gccgaaggga tcggtatccc gctggatgcg acagcagatc ggaccgcacg
    38701 caaggacagc gccgatcccg gaaagtccga ttctgaccag cccggccggt gcggcggggg
    38761 caagcgggtc cgtcggttct gtcgggccga tggcgagcag aagccgccac agcgaagcgc
    38821 ccgaaccgat cccgtaccgt tcgatgctgg cttcgccgac gtcccgtcct cggctcttca
    38881 ggggacgggt ggcaaggcgc tctccgtccc cgagcgtcca ggacaggtcg tgctccgacc
    38941 cacccggcag ggcgacatcg atcgtcacgc cagccgggct ggcacgatcc ggcagccaga
    39001 gttccaggta gttgctggtt cggtcgccgg gctggatgcg ccaggggatg gtgaaggcat
    39061 ccggcttgtg accgcgcgcg ctgagcatcg ccgccgagat ctcgccataa agtctggcat
    39121 gaaacgtatt tcctgccggc atcaccagat gtgtcggctg agtggcggcc cgtttcgcca
    39181 ccagcgtccg gatagcacgt tccaaccggt ctcggccgcc atgcgggccg ccggtaaagc
    39241 cgtagctgaa gttcacgaca agcggcagcc tgtcgatgcc atgcgccccg gccaggcgat
    39301 cggtgcggtc gaatatgtag tgcagcgcgc tcagtatgaa tgcgtccttg ccaaccccgg
    39361 ccgtatcgag cgtcaggggt tcgggcaact gcacggcaat gacggcgacg tcgtccatgg
    39421 ccgcgccatc ctgtccggcc gcgaggccgg tgacatggga gccatgggag ccgcgccgat
    39481 caagtgtggc atagggatag gcgttctccg tccgcgcgcc ggccaggtcg tagaacaggt
    39541 cctcgtcgcc gccggccatc gtcagcagcg catcgatgtc gggggccagg aattcccggc
    39601 cgaaaagcac cgtccgctgg gctgccgtct cggcggtcga cgcggactgc atccaacaga
    39661 aggcgacacg actgcgttcg ccgtccgcgc tccgcaacgc cttgtgagcg aatgggatac
    39721 cgtcctcgat caccgccacg acgacctttg gcttcgccgg cgggtgctga ccgagccgcc
    39781 gctcaattcg gcggggatca taggccgtat cgaccgcccg ggaggggacc ggcatgttca
    39841 gtcgaagtcc ggtttcctcg cgcaatccct tcgttccgcg cagagcgccc gcgtaggtgt
    39901 cgacgagccc gttggccccc gcgacccggc tggcggcttc caggatcacc ggccagaacg
    39961 caacgtctcc atcggcgtgc gcgccattcc agagctgcgg cacgagcacg cggcgttgcg
    40021 tgtcccgcct cgtgcccttg gcgagcgctt ccgggcgcat ccccgccggc aatgccgcga
    40081 gggcggcaat gaacggggcc tcgcgcgcct gggacgttcc gaagacgtga ggcgccccaa
    40141 ttccataggc ccatgcctca taggcaccgg tcacttcgcg cttgggactt ggcattggcg
    40201 tcggcccccg attaccagga atagcgaata agtcggtcag ggcagtttga attcgctgac
    40261 ggcctcgttc cagagccact ggaagtggtc gcttggctcg atcttgccct tcgccggcct
    40321 gcggtcacgc ctcaacccgg tctcggccgg gctcgcaggg cgtccgcccc agatttccgg
    40381 cgcgaagcgc gcgaccgaga agtcaatgtc cctggcggca tagctgcgcg gaattggcgc
    40441 gcctttgccg catttcgata ggattgcctg agcgactgcc tcccccagcg cggcgcccgc
    40501 ccacgtatcg atggggaaat gcatgcccgc gaccgtccgg ttgaccgcga tccgctcggc
    40561 ctgtttgaac agcagtttcc tgcggttcgt gacatcggga tagtgccgcg tgacgtcggc
    40621 ctccgacaga agcgtagaaa gcagcgtcgc ggcggcaaag gactcggctg catgcgcgct
    40681 tggaaacgcg ccgtgcccgg gcgtaggaat caggggcatg atcgtgccgc tcaggcgatc
    40741 cggacgccgg caggccaggt gatgtttggc caccatggcg acatgggcgg tcaggacctg
    40801 tgtgatcagg atcagttcga aggtgcgcgg attggtcgag gcgttcagcc cgaggatcga
    40861 ggcgaaataa tccagcggga agccaacctg cgtcaggatt tccacctggc gatcggcgcg
    40921 ctgatccatg tagctgcgca cgagttcgac ctgccgctgg aagagcgccg gatccgggcg
    40981 cgccagctcc acaaggcggt ttcccgcata gcggatttcg atcgagccgc catccttggg
    41041 acgggctgac cagctcagat ccttcaggaa atccgtgaga tagagcgatg cccgcaccca
    41101 accggcccaa agcgtcatgt tcgcgctgct tgaaaacgcc agcgcatcgg ccgccgcgac
    41161 cccttcgtct ccgacatgca ggatggcctg ggcgcttgtc atcaggcctg tcgaaaccgc
    41221 cggatccccc gaaccacccg atacgaagcc accgatacca ccgatgccgc caatgccacc
    41281 gatgccaccg atgccgccaa ttccaccaat tccaccgatg ccgccgattc caccaattcc
    41341 gccgatgccg cccatccagg tcattgtcga atctccggga agttgaagtt atggaatgcc
    41401 tgcggcggtc agcgcctttg cggcgagtcg tccgaaatcg tggtcagctg cggggtggct
    41461 gctgatgtag cctgcaacgg tcaggtcggg ctggcgcctg agcaactcgc cgccgacgat
    41521 cttggcttcc gcgatctgtc ctagaccgta aagggcaatg atctttgccc ggtaggtgga
    41581 caggtggcgg tcgttcgcgg ccagtgatcg gttggcaagc tcgagcccgg acctgtaatc
    41641 gccgtgcgca aggcgcgccg tcgcgtcgag agaatcgtag aaatagccaa agggatcgag
    41701 cggagacagg cggcgcgcac gatcagttgc gccgatcgca ttcgtgctct ctccccgaaa
    41761 ggcatgcaag gcgcccttga gcagccaggt cagggaggaa ttcggattgg cggccagtgc
    41821 cagatcgtag cgcttttcgg cgatgtccag ccgccggagc aggttactgt gtgcaaatcc
    41881 gtcaatcgtc aggcagaagg cattgtccgg actgaggtca agcccgcgcg cggtgtggtc
    41941 aaccgccagg cgcgtgtcct tggcgatgtc ggtgctccag ccgttgaaca cgctgagcac
    42001 gcaccatttt ccaagccagg catggacttc aggcgcatcc ggcgcacggc gcgcagcttc
    42061 attcagcagg tcgtgggatc gcatgaactg ccgcaacgtg gcgcggtgca tcaggccgat
    42121 accggcgatc agcaggcggt ggtcgtcgat ttccggcggc gagcgtccaa tgacatgggt
    42181 cagggcgtcg tcggcgatgg caccgctgat cgaggtcagg atggcggcaa gcgcttcgcc
    42241 ggatcgatca aggaacgcgt catacggatt taggaactgc cgggtccaca ggatgcgacc
    42301 ggagcgcgca tcgatcaggt cggcgtcgag cacgatctcg gaccgggtcc gccgcaggct
    42361 gccgctgacg cagaaatccg ccttgagcag gtcgcggaca tgcgacatgt ccacgccgga
    42421 gctgtcgacc tgccggcagg acagatggct gatgacatcg aataatcgcg aacggctcag
    42481 ggttcgcgac aattcctctg caatccaatc gccgaacacg gcgtcggaac gatcaccgcc
    42541 aatggcgcga aacggcagga ccgcaacccc cggcaggatg cgcgagacat gcggccggtc
    42601 cgtcaggctg aacaggccgg cgagctggcc aggattgttg cggatgccga ccagccattc
    42661 gttgaacagc ggttcgcgga tctcgatccc ttcgaggaag aggccggcct gtaaatgccc
    42721 catgaagcgc acgcgcgaca ggtcgatggt cagttcgaga ttgttgctcg aaagcacctc
    42781 cgaataggcc tctcccatga tctgcttgat gtcggacagg gcgcgccgca ggctttggcg
    42841 gcccgtgtcg tagcaagacg ccccccaaag cgtttcctgc aggaaagccc gggtgcggcg
    42901 gccgaacgga gccgtcgcca gcagggcgaa cagcgccttc tgcttggtcc ccgtaatgca
    42961 gaatcgtccg ggcgtcgcgg actcgaccga acacgctccg aagagattca cgtcaaggac
    43021 cttgcccacc atgattgcgt cagcccgtcc cacaccaaga atatgctagc attgcgtaga
    43081 attccgggac aaggcggctc acgatttact cacgcgcctc gcgttatcga ccgcactaac
    43141 atgcttgcat ccatatgcgg gccctgcgcc ccgcacgcag gaggaaggct cgatgtccgg
    43201 ccaaacatca actgcgctcg cgcatgtgcg cgatctgatc gattttgtca ggcactggga
    43261 tacctatcgt gacgtggcgc agacagcttc cggcagttcg gcgctgacga ccgaacagaa
    43321 agccgtgatc gactggttga tcatgctggc cgatcgtgtt ggccc
//
LOCUS       DAOMXN010000008        41781 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_656_length_41781_cov_13.275176, whole genome shotgun sequence.
ACCESSION   DAOMXN010000008 DAOMXN010000000
VERSION     DAOMXN010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 41781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41781
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_656_length_41781_cov_13.275176"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PK812_01775"
     CDS             complement(<1..188)
                     /locus_tag="PK812_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HRJ68308.1"
                     /translation="MLDPDFLLGPSTRGARFMLEFEKADEVLRAHRIASTLVVFGSAR
                     AREGAPGAMGRWYDAARA"
     gene            328..1410
                     /gene="pheS"
                     /locus_tag="PK812_01780"
     CDS             328..1410
                     /gene="pheS"
                     /locus_tag="PK812_01780"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018264367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HRJ68309.1"
                     /translation="MTDLPSLQAAILADIAAAGDEAALESVRVSALGKKGSISLQLAG
                     LGKLAPEDRKSAGAAINAVKDAVGEALSARKSVLKAAALDARLKAEALDVTLPVQPSA
                     LETGRIHPISQVIDELTAIFADLGFAVAEGPDIETDDLNFTKLNFPLGHPAREMHDTF
                     FFPPDANGERKLLRTHTSPVQIRTMLAQKPPIRIIVPGRTYRCDSDQTHTPMFHQVEG
                     LVIDKTANLGELKWVLAEFCKAFFEVPNVTMRFRPSFFPFTEPSMEVDIQCRRQGGEI
                     RFGEGEDWLEILGCGMVHPNVLRNCGLDPDEYQGYAWGMGIDRIAMLKYGMPDLRPFF
                     DADVRWLTHYGFRPLDLPTLAGGLTG"
     gene            complement(1407..2222)
                     /gene="phnE"
                     /locus_tag="PK812_01785"
     CDS             complement(1407..2222)
                     /gene="phnE"
                     /locus_tag="PK812_01785"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010687323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HRJ68310.1"
                     /translation="MKETQAPPDQARRWQRFSPAERIARSMLAIVTAVAVVWSIKTID
                     IIPEFLYDAPEQTADLFKRMWPFDWGFYPKAVHDALIETFHIATLGTMLAIVLALPLS
                     FLAARNFTKSPVLNFLAQFIFVSSRSVNSLIWALLFVAIFGPGALSGVLSIAFRSIGF
                     IGKLFGEALEEAQRGPIEALTAAGAPRSMILAKGYWPQVEPAFWSVALLRWDINIRES
                     AVLGLVGAGGIGVALDSALNNLYWDQVGLILAVIFLVVIATEIVTSTIRKRII"
     gene            complement(2219..3016)
                     /gene="phnE"
                     /locus_tag="PK812_01790"
     CDS             complement(2219..3016)
                     /gene="phnE"
                     /locus_tag="PK812_01790"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HRJ68311.1"
                     /translation="MSLAASRPRPPAFPVNWPARLAWIGLALYALYATSILNLTWARF
                     VVGLDNGAKFLDRMFPPNMQADKLELIGSGMLESIQIAVLATFFGVLLSLPLGLMAAR
                     NLAPGWLSWSARGLIAVLRSFHPVIVSILFVKAVGFGALAGILALIVSSMGFVSKLFA
                     EVVEEMNAKQVEAVRATGANFWNVLIFAVMPQVMARFVGLSVYQLDSNLRNSTMVGIV
                     GGGGIGAPLFTAYQRFDYDVVLTILICIIALIMLAEVVSGRIRRIFQ"
     gene            complement(3013..3774)
                     /gene="phnC"
                     /locus_tag="PK812_01795"
     CDS             complement(3013..3774)
                     /gene="phnC"
                     /locus_tag="PK812_01795"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HRJ68312.1"
                     /translation="MNSALLTIRNLRKEYRLGQPVLKDISLDIDAKGVTAIIGPSGTG
                     KSTLIRCINRLVDPTSGEILFAGQDLAKLSGAPLRAARRRIGMVFQEYNLVERLSVIE
                     NVLSGRLGYVPAWRAFLRRFPQADIARAFDLIDAVGLNQDFATRRADALSGGQRQRVG
                     IARAIMQDPALVLADEPTSSLDPKTSVEIMELLAELSDKRGVPIIVNIHNVGLAKRFA
                     GRIIGMTGGEVVYDGPPDGLRDDHLKHIYGGEDFL"
     gene            complement(3771..4811)
                     /gene="phnD"
                     /locus_tag="PK812_01800"
     CDS             complement(3771..4811)
                     /gene="phnD"
                     /locus_tag="PK812_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020091223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_function: GO:0043225 - ATPase-coupled inorganic anion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HRJ68313.1"
                     /translation="MIVAAAVAAAFPALAQDSCPNRGQLDMAYCDANNDLVADLPTDP
                     SRHKDPATLIFAYSPVENPAVYQTVYQPFMDYLGSCTGKRIVYFPVQNNAAQIEAMRS
                     GRLHISGYATGVVGFAVNLAGAVPFAVIGSDKGVSGYKLFALVKADSPYKTLSDLKGK
                     KVAHTSPSSNSGNLAPRVFFPDNGLKPEEDYKVLMSGGHDKSVLGVASGDYDMAPVAS
                     DVYERMVARGAVKADALRVIWQSETFPTSSWSIPHDLKPELAKRITACFMDYKWHEGL
                     KKEYAGEDRFLPMTYKDTWRPIRDVAEKSGTPYNRAAYDAQTKREADAAAKKAAEAAA
                     KAAAGQPAAPKQ"
     gene            complement(4832..5905)
                     /gene="phnD"
                     /locus_tag="PK812_01805"
     CDS             complement(4832..5905)
                     /gene="phnD"
                     /locus_tag="PK812_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_function: GO:0043225 - ATPase-coupled inorganic anion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HRJ68314.1"
                     /translation="MQQSLVIRAFLTATALAGLSVTALAQDACANRGQLDTLYCDANN
                     DLVADAPADPKKWRDPSTLVWAYTPVEDPAVYANIFKPFTDHLEKCTGKRTVYYPVQS
                     NSAEIEAMRSGRLHFAGFSTGPTGFAVNLAGAVPFAAKGLGNEVRGYNLIAIVKASSP
                     YKSLADLKGKKVAHTSPSSNSGNLAPRVLFPEQGLKTDDDYKPLMSGGHDKSVLGVNS
                     GDYDMAAVASDVFDRMVTRGTVKGDDFRVIYRSPVFPTSSFAHAHDLKPELASKLKEC
                     FYSFRFTPAMTKEFNGDDRFLPIAYAKDWKVVRDVAEKSGTPYNKAAYDAEAKREADA
                     AAKKAAEAAAKAAQPATTTPPKQ"
     gene            6111..7442
                     /locus_tag="PK812_01810"
     CDS             6111..7442
                     /locus_tag="PK812_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009339488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRJ68315.1"
                     /translation="MISRRVALAAAALSVALAGAAHGQAPSGKVTVVTSFSKEVTDPF
                     KAAFEKAFPGTTLEVQNRNTNAGVKFIEETKSNNGVDLFWASAPDAFEVLKAKGLLEA
                     YKPKADGIPDKIGHFPINDPQGFYSGFAASGYGIMWNDRYVKANKLPEPKEWEDLATA
                     PYFDHVSMAAPSRSGTTHLTIEAILQSAGWEKGWGIVKAFSGNLRGVTERSFGVPDAV
                     NSGQVGYGIVIDFFGFSAQGAGFPVKFVYPSLSTIVPANIGVVKNAPNAAGAKAFVDF
                     ILSEPGQAILFDPKIRRLPVRTASYAKAPAGLPNPFEFKWLADPKSKFDVEKSEKRFA
                     AVDALYDQLVTFQHEALKAATKAIHDADAAIARKPSPEASRLVAEARALVARMPVSEA
                     EAASPEMAAAFTGGKEKGPRQAELEQKWAAYAKENYAAALAKAQAAQQAAK"
     gene            7552..9228
                     /locus_tag="PK812_01815"
     CDS             7552..9228
                     /locus_tag="PK812_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013169069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRJ68316.1"
                     /translation="MASPGLALLALLIGLFLAAFLVIPVVTVIFVAFQPKGSTGFTLI
                     NFVDFFSTDLFRRSFWNSAYVSLMSVVIASLLALPLAYYTTRFEFRGAALVQTLGVIP
                     LIMPPFAGAVAMQLLFGRNGTVNLLLDDWFGFKVPFMEGLNGVIFVEAVHYFPFILVN
                     LSTSLRNIDRAMEEAAQNLGCSGFRLFRRIVFPLAMPGYVAGASLVFIKVFDDLATPL
                     LLNVKDMLAPQAYLRITSVGLADPMGYVISVVLIVASLLAMWLSTLALRGKDYATVQR
                     GGGGTVKRPLRGIDRVLAYGAITVVLVLVLAPHVGLFLLSIATIWSFAPLPDGYTAAH
                     YARVLSESGQYITNTLIYAGLAGLIDVVIGSAIAYLVLRTGMPAGRWLDYVATAALAV
                     PGVVLGIGFLRTYFGVTLPSGQSLAAFWGMIVLALAIRRLPYALRACMAAMQQISVSL
                     EEAAQNLGATRSRTVRRIVMPLMLSGVAAGFVTSFATAAVELSATMMLVQTQSDAPLA
                     YGLYVFMQSPAGRGAGAALGVIAVVIVGLSTYVSNRLVERDMAARSEAKE"
     gene            9225..10217
                     /locus_tag="PK812_01820"
     CDS             9225..10217
                     /locus_tag="PK812_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013169070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ68317.1"
                     /translation="MTALLQPVGIDVEGVDLAFGATQVLRNVSLSVHPGEFFAFLGPS
                     GCGKTTLLRLIAGFNVAQKGRVLIGGRDVTALPPWRRDVGMVFQSYALWPHMSVARNV
                     AFGLEERRLPRSEVAGRVDEALDLVGLRHLKDRKPSQLSGGQQQRVALARTIAVRPQV
                     LLLDEPLSNLDAKMRVEVRRELRELQQRLKLTTIFVTHDQEEANTICDRIAVMSEGIV
                     QQVGAPMDLYDRPANLFVAGFLGTANFLEEGGRKVMVRPQNVQLGAEAATGAFMISGA
                     IVHREFQGATMRYVLETERGPLIVDEPYDPGRSPYAVGSQVNAAIRPERLVAVA"
     gene            complement(10315..11190)
                     /locus_tag="PK812_01825"
     CDS             complement(10315..11190)
                     /locus_tag="PK812_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007610662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF296 domain-containing protein"
                     /protein_id="HRJ68318.1"
                     /translation="MARPGYVRQPGAAIAARVSAAPAQGTALTLDLPAGLPLLEAVRR
                     ALARTGHDSGVVDLRDLALGPFGYVMPALAENGRHAAFYSPVFRPAGMTRLAAGAMTF
                     GLRDQSPFFHAHALWREQDGKQGGGHLLPEETLLAEPAQVEALALTGATFTWRLDPEI
                     NFTVPGPAAASAPGHDRTAHALRVHPNVDLHRAIEDYCVRHAIAHARIRGGVGSTIGA
                     LFDDGREVTNFATEVFIERGSIAPGTNGLPVATIDVGLIDFSGRVERGRLQRGANPVL
                     MTFELALEVTGVSTR"
     gene            complement(11192..12826)
                     /locus_tag="PK812_01830"
     CDS             complement(11192..12826)
                     /locus_tag="PK812_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HRJ68319.1"
                     /translation="MTKGSAPAGGVPRCSRRVMNLAHILTQNRRRLPDHRGLIHGDES
                     WSWHAIDGAVTALASALAARGIAKGDRVLVHSKNCAEMYFSMFACFRLGAVWVPTNFR
                     LLPEEIVYLATFSGAKAFLCHGDFPDHAAMIAHRAANVDFIWRMGGSASFGETTVGAA
                     IARHGGAVLENAPVDHDDPCWFFFTSGTTGRSKAAVLTHGQMAFVITNHLADLLPGAT
                     EDDASLVVAPLSHGAGVHQLDIAARGAPTILLPSERFDIPEAFRLIQEHRITNMFTVP
                     TILKMLAEHPAARTYDHSSLKHVIYAGAPMYREDQKTALRALGKCIVQYFGLGEVTGN
                     ITFLPARLHDEADGPAARIGTCGFERTGMQVSIQDDAGRELKPFETGEICVIGPAVFA
                     GYYDNPEANAKAFRDGWFRTGDLGHLDEQGYVYITGRASDMYISGGSNVYPREIEEKI
                     LMHPAIAEVAIVGVPDPLWGETGIAVCVLRDGQCVTEDEIVAFLTPKVTRYKMPKRVF
                     FFEALPKSGYGKVPKRMVRDELEARGLLTPEQLIRI"
     gene            complement(12914..13516)
                     /locus_tag="PK812_01835"
     CDS             complement(12914..13516)
                     /locus_tag="PK812_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRJ68320.1"
                     /translation="MARPLAADYGDKRKAILKASARLFARDGYDRASMADIAAECSIS
                     KALLYHYYDTKEQLLVAIIREHLEELVAATLPPAGGSPRARLRALVGALLDCYRDADH
                     EHQIQINELKKLPKPRAEELVELERVLVRRFSAAVAAAVPSLEKRSALIKPVTMSLFG
                     MLNWHYMWFREDGPITREAYADLATDLIVEGAQKAAARGS"
     gene            complement(13521..14831)
                     /gene="paaF"
                     /locus_tag="PK812_01840"
     CDS             complement(13521..14831)
                     /gene="paaF"
                     /locus_tag="PK812_01840"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HRJ68321.1"
                     /translation="MIIKNSYKPMVDPIEVASRDEITGLQLQRLKWSLRHAYDNVPAY
                     RKKFEAAGVHPDDMKSLSDLARFPFTTKADLRDNYPFGMFAVPRDKVARVHASSGTTG
                     KPTVVGYTLGDIAVWSDVMARSIRASGGRPGMMVHVAYGYGLFTGGLGAHYGAEKLGC
                     TVVPISGGMTERQVQLIADFKPEIIMVTPSYMLAILDEFRRVGIDPRATSLQVGIFGA
                     EPWTNAMRQEIEQAFDMDAVDIFGLSEVMGPGVANECVESKDGLHIWEDHFYPEIIDP
                     ETGQVLPDGEMGELVFTSLSKEAMPVIRYRTRDLTRLLPGTARSMRRMEKVTGRSDDM
                     MILRGVNVFPSQIEEKLFAIPELSAHYQIVLTREGRMDQMEIKLEARDGSVTDQDRKA
                     AAARLAHMIKSTIGVSTRINVGDPGAVERSAGKARRIIDNRPKV"
     gene            complement(14851..15291)
                     /gene="paaI"
                     /locus_tag="PK812_01845"
     CDS             complement(14851..15291)
                     /gene="paaI"
                     /locus_tag="PK812_01845"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006933316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HRJ68322.1"
                     /translation="MTANELARACADAMWAEDKASRTLGMALGAVSAGRAVLTMPVTE
                     TMVNGHGICHGGYIFTLADSAFAFACNTYNARTVAQSCAITYIAAARLGDVLTATGTE
                     VSRTGRSGIYDIAVQNQAGATIAVFRGNSRTIKGEFFPGVIIVS"
     gene            complement(15308..16099)
                     /gene="paaG"
                     /locus_tag="PK812_01850"
     CDS             complement(15308..16099)
                     /gene="paaG"
                     /locus_tag="PK812_01850"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HRJ68323.1"
                     /translation="MTDPVLVEDCAGYRVLTLNRPDRLNSFNEAQHRLLKSAVDHAAA
                     DPACRAVLLTGAGRGFCAGQDLSDRVQGEGGDAPDLGRTIEAFYNPLVRAIRGMDKPV
                     VCAVNGIAAGAGANIALACDIVLAARSASFLQAFSRIGLVPDSGGTWFLPRLVGEARA
                     KALVLLAEPVKAEEAERIGMIWKAVDDAQLMADAHALCARLAQAPTYGIGLAKRALQA
                     SHANTLDQQLDLERDTQRRAGVSADYAEGVRAFMDKRKPNFTGRP"
     gene            16248..17915
                     /gene="paaN"
                     /locus_tag="PK812_01855"
     CDS             16248..17915
                     /gene="paaN"
                     /locus_tag="PK812_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016557509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaN"
                     /protein_id="HRJ68324.1"
                     /translation="MPALFEKHRPLLEQAIAAARSRANWSAYPESQSPKIYGEDAKAN
                     ADNAFEALKGKPFDLPGHPGGKHVGGEVSPYGFALGVTYPQAEVDVLVAASKAAGAKW
                     GAASPEERAGVLLEIIARINRASFLMAHAVMHTSGQAYSMAFQAGGPHAQDRALEAVA
                     YAFDEMSRVPGAVRWSKQTGKDQVTTLDKTFRIVPRGVALVIGCATFPTWNTYPGMFA
                     SLATGNTVIVKPHPGAILPLALTLGIAREALAEAGFDPNVVLLAPDTIDRPLTKELAL
                     HPGIGIIDFTGSPVFGAWVRENAKGKLVYTEEAGLNSIVIASTDNFRAMATNIAFSLT
                     LYSGQMCTAPQNIFVPAGGIETDQGHKSFDEVADAIKSGIDKLLADPDRASAFLGAVQ
                     SEATAQRVKEAAKLGRVVRSSDSPVYSAAPGARTASPIILAVDAADEPAYMAERFGPI
                     SFVIAVRDGGDGIARAAAAARQHGAISAALYATDEATIDRAIGAFAEAGVTLSINLTG
                     GVFVNQNAAFSDFHVTGANPAGNACLTDAAFVSNRFRVVGTKRQVAA"
     gene            17979..19127
                     /locus_tag="PK812_01860"
     CDS             17979..19127
                     /locus_tag="PK812_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRJ68325.1"
                     /translation="MTRLKRLIGVGVVASTAILAAAAAQAQIKIGAVLSVTGPASFLG
                     DPEKRTLDIYVEQINAAGGINGQKIEMKVYDDAGDANAARTFATRLVEEDKVMAVIGG
                     STTGTTMAMMPVFEDAKVPFISLAGAVQIIQPVRKFTFKTPHTDTMACEKIFQDLKAR
                     NLTTIAMISGTDGFGASMKAQCEAVAGKSGITIAHQESFGPRDTDMTPQLTNIKGKAG
                     VQAVVVPGFGQGPAIVTRNYRQLDIKLPLYHSHGVASKQFIDLSGPAAEGVRLPAAAL
                     LVAEKLPDSDPQKKVVVDYKATYEKRTGQPVSTFGGHAYDGLMILVDAIKRAGSANAE
                     KVRDAIESTKGYVGTGGIVNMSPTDHMGLDLSAFRMLEVSRGEWTLVQ"
     gene            19300..20172
                     /locus_tag="PK812_01865"
     CDS             19300..20172
                     /locus_tag="PK812_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRJ68326.1"
                     /translation="MPELMQFVFSGLTVGAVYALVALGFTLIYNASDVVNFAQGEFVM
                     LGGMITVFLGLAGLWLPLAAMLAIILTVFVGLALYKLAIEPARGAAAVTLIIITIGAS
                     IFLRGIAQIVFDKRFHALPHWFGQQPLKFGGASILPQSLVVLAGALVIVVLLYGFFER
                     TLFGKAVLATAANRLAARLVGINTDVVVGFSFAVSAAIGAIAGLLVAPITLTSYDVGT
                     LLALKGFAAAMLGGMGNPVGAVVGGLVVGLLESLAAGLISSKYKDAIGFIVILFVLFA
                     MPNGLLGKTKVERV"
     gene            20172..21116
                     /locus_tag="PK812_01870"
     CDS             20172..21116
                     /locus_tag="PK812_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRJ68327.1"
                     /translation="MQRFLSHRLTVVVVLALIVALLPLVFPSAYYYRVAGQVFVFGLA
                     AIGLNLLMGYAGQVSLGHAGFMGIGAYAAAIGPAQTGTPVWFWVVAGSALSGLIAWVV
                     GRPILKLRGHYLAVATLGFGILLFMVFTNESRWTGGPDGMPVQRLELFGWRVRTGIEW
                     YWVSGVLLVLGAWVAGNLVDSPTGRAMRAIHDSEVASRSAGIDVARQKLTAFVISAVY
                     ASVAGASFALMNGHLTPDVSSFLRSVELVTMVVVGGMGSIFGSLVGSAVLVVLPQALT
                     VFHDYEHAILGLMIMLFMIFLREGIVPSLARRFGRRSA"
     gene            21113..21898
                     /locus_tag="PK812_01875"
     CDS             21113..21898
                     /locus_tag="PK812_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ68328.1"
                     /translation="MSKLLSVSDLSISFGGVQAVDGVSFEARTGEIYAIIGPNGAGKT
                     TLFNMVSGVYRPDRGRVMLDGQDVTELEPDRLCRLGLSRTYQNLQIFFRLTAAENVMV
                     GRHRHESANVLAHLLALPSVARQNRETRRNAVALLARIGMERYADTPAGSMPYGALKR
                     LEIARALATEPRFLLLDEPAAGCNPVETDEVEAVIHAIAKDGVGVVLVEHDMKLVMRI
                     SDRILVLEQGRPLAEGQPRDIRENPAVMKAYLGEHGAREAARA"
     gene            21891..22592
                     /locus_tag="PK812_01880"
     CDS             21891..22592
                     /locus_tag="PK812_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003498150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ68329.1"
                     /translation="MLEVANLTSSYGRIEVLHGVSLTVAAGEIVTIVGANGAGKTTLL
                     RAISGVQPITAGTISFEGRLIQSIAGHDRVGLGIAQVPEGRQVFAPLSVEDNLRLGAW
                     SRRGADVLDDIERAYAMFPVLKEKRHQHAGALSGGQQQMLAIGRALMSKPKLIVLDEP
                     SMGLAPILVDQIFGVIRAFKQAGQTVLLVEQNAFAALSIADRGYVLETGRIALSGRGA
                     DLVRDERVRATYLGQ"
     gene            22632..23942
                     /gene="hmgA"
                     /locus_tag="PK812_01885"
     CDS             22632..23942
                     /gene="hmgA"
                     /locus_tag="PK812_01885"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HRJ68330.1"
                     /translation="MALSYMTGFGNGFETEALPGALPVGRNSPQRCAYGLYAEQLSGS
                     PFTAPRASNERSWLYRIRPTVRHWGRFRKADIGLWRTAPAREVEAPPAPYRWSPLPLP
                     SESVSFLQGVRTITTAGDAGATAGMSASIYLVTRSMVDECFYNADGELLFVPEHGGLR
                     LVTEFGIIEVTPGEIAVVPRGIKFRVEVPDGQGAARGYLCENYGGAFTLPERGPIGAN
                     CLANQRDFLTPVACFEQQDKPTAMYVKWGGALWVTDLKHSPIDVVAWHGNYAPYKYDL
                     RRFSPVGPLLFDHADPSIFTVLTSPSETAGTANIDFVCFPERWMVAENTFRPPWYHMN
                     CMSEFMGLIYGQYDAKPEGFVPGGFSLHNTMLPHGPDHDSFEMNSTVELKPHKLANTM
                     AFMFETRFPQAITAYALNAPELQHSYGDYGHALKDHFDPHRRDR"
     gene            23951..24976
                     /locus_tag="PK812_01890"
     CDS             23951..24976
                     /locus_tag="PK812_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019857519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRJ68331.1"
                     /translation="MKLATLKDGSRDGRLVVVSQDLTRHTDAAGICRTLQQALDGWER
                     YAPALADLAEQLEHGSVPAQRFHEHDCASPLPRAYQWADGSAYVNHVELVRKARGAEM
                     PPSFWTDPLMYQGGSDTFLGPRDPVPVATEAFGIDLEGEVAVIVGDVPMGATPEIAAR
                     AIRLVMLVNDVSLRNLIPNELAKGFGFFQSKPSSAFSPVAVTPDDLGPAWVGGKVRLP
                     LLTFINGKPFGKPDAGQDMTFDFPTLIAHAAKTRPLSAGTIIGSGTVSNKDRDGGPGR
                     PVADGGLGYACVAEIRMVETIRDGKPTTPFLSFGDVVRIEMKDARGKSIFGAIEQEIV
                     AHTGSQA"
     gene            24973..25926
                     /locus_tag="PK812_01895"
     CDS             24973..25926
                     /locus_tag="PK812_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRJ68332.1"
                     /translation="MSGFASTNDLADKKVSFSEIGPGLYAYTAEGDPNTGVIIGDDGV
                     MIVDAQATPAMAQDVIAKIRSVTDKPVRYVLLSHYHAVRVLGASAYTDAHAILASDRT
                     RALIVERGAQDMDSEVGRFPRLFRGKETIPGLTWPTLTFTGEISVWLGKREVRIAQPG
                     RGHTAGDTIAWVPDAGVMFSGDLVEYKSACYCGDAHFRDWPETLKKLKAYDPVALVPG
                     RGDALVGGQKVSAAIAGTTDFLAALYGAVEASVKRGESLKEAFAAVRKAMDGPFGSYA
                     IYEHCLPFNVSRAYDEARGIDHPVIWTAERDVAMWKALQAG"
     gene            26078..27016
                     /locus_tag="PK812_01900"
     CDS             26078..27016
                     /locus_tag="PK812_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HRJ68333.1"
                     /translation="MSMTQLNAEDVRRRLPWDRLIGAIEAQFSEGAQVPPRPVFDVAN
                     PKGQAGALLLMPAWTEGETLGVKVVTFFPGNSELGLSTISAAYLLFDAATGQIRAVCD
                     GDEITVRRTAATSAAAARRLARSDAKRLLVVGSGQLAPNMAEAHAAVRGYDVIEIFGR
                     SRDKAAAVVSRLVRAGLPARVADDLEASVRAADVVSCCTSATVPPVQGTWLRPGTHVD
                     LVGGFRDDMREVDDTAVARASLFVDERPGALKSGDLAQPIRAGLLRESDIRADFRELV
                     TGSHVGRTSAQEITLFKSVGHGLEDLAAGRMIARTL"
     gene            27118..27825
                     /locus_tag="PK812_01905"
     CDS             27118..27825
                     /locus_tag="PK812_01905"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012322211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HRJ68334.1"
                     /translation="MSTALLPTDARAIARQTAKMLLEIEAVLFRADQPFIFTSGWASP
                     VYTDCRKIISYPRLRAALMDFCAATVMREVGYEQIEVIAGGETAGIPFAAWISERLGL
                     PMQYIRKKPKGFGRNAQIEGNVVEGQRALLVEDLATDGRSKVNFVNALRTAGMTCNHG
                     IVIFYYDIFPSGKQVMKDLDFQLLHLATWWDVLDVAKEGRYFDAGVLREVESFLHKPA
                     EWSAAHGGISEFGKAGE"
     gene            27910..28866
                     /locus_tag="PK812_01910"
     CDS             27910..28866
                     /locus_tag="PK812_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRJ68335.1"
                     /translation="MSLARRIRLPRTDLDVSPICLGGNRLGGELDQAASFALLDAFVA
                     AGGNFIDTAHVYANWLKDAERSCSEKTLGRWLRARGGTRGVVIATKGGSPEAEAPEVR
                     RLDEGSLRRDLAESLEFMGVSTIDLYYLHRDDPARPVEELLALLEDMKTKGQIRHYGA
                     SNWSVARLEEAESVCRARGWSGFAANQCEWSFAKRNAGTTGADMVQGDAAMAAWHRRH
                     GIAAVPYSSQAKGWFDKVATGRQEPLTDRLYDNAGNRALAPVLAAAAQAVGSTPTQAM
                     LARMMQAPFPFVPIIGPRSVEQMRSSVASLALPPVEHGLPAV"
     gene            complement(28871..30007)
                     /locus_tag="PK812_01915"
     CDS             complement(28871..30007)
                     /locus_tag="PK812_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018259897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRJ68336.1"
                     /translation="MILTAEQEMIRDTIRAFAQEQLWPNAARWEREKTFPREALQALG
                     ALGALGMTVPAKWGGAGLDYVSLVLALEEIAAGHGATSTIVSVQNSLACGIPLAYGTD
                     EQKRRYLTKFASGAWIGCFCLTEPHTGSDASALRTTARREGDAYILNGTKQFITSGKH
                     GQAALVFAVTDAAAGKRGISCFLVPTDAPGYIVSRVEDKMGQHASDTAQIILENCRVP
                     ADNLIGSEGEGYKIALANLESGRIGIAAQCVGMARSALEEAVRYANDRKAFGVPIVQH
                     QAVSFRLADMATRLEAARQLVWHAASLKDAGRPCLKEASMAKLFASEAAESICSDAIQ
                     IHGGVGYTADFAVERIYRDVRVCQIYEGASDIQRLVIGRAVAGG"
     gene            complement(30104..30742)
                     /locus_tag="PK812_01920"
     CDS             complement(30104..30742)
                     /locus_tag="PK812_01920"
                     /inference="COORDINATES: protein motif:HMM:NF036525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRJ68337.1"
                     /translation="MPAESAILPAETPADRRREEVLAAAAECFMQKGYENTSMDDVAE
                     RLGATKGRVYHHFNSKPELFFAVYRRAMDMLTQAVAPAVSRPGLAGDRLRDMAQTHAL
                     TLMESLPFQRTAALAPDLWRFGRAAEHQATLSDLIMLRDIYEALFRRVLEDGARDGSL
                     RRFPEAALASRTLLGALNWVAVWYQPRPGEGAAERRTLAATIAETVLSGYRA"
     gene            complement(30732..32372)
                     /locus_tag="PK812_01925"
     CDS             complement(30732..32372)
                     /locus_tag="PK812_01925"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009467773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HRJ68338.1"
                     /translation="MLRGMMMDVPLNVSTILDYAAEMHAESEIVSAALEGGFHRQTYA
                     QAAQRSAQLAHGLKALGVKPGDRIATLAWNGYRHFELYYAVMGLGAVCHTINPRLFPE
                     QLTYIVNHARDKALFFDINLAPLVAKMRAHWPKDLKLVALCDRGHLPTGEGLEGVLAY
                     EDLLAGQPRSMAWPSLDENTACCLCYTSGTTGEPKGALYSHRSTVLHALFGMTTGIAG
                     LRPGQTILPVVPLFHVNAWGLPFSAPMIGVNLIMPGARLDGASLFDMMEAEKVFSAWG
                     VPTVWLGLLAEIQKRGRKPNGFGEVIVGGSAAPASMIETFEKMDVSIVHAWGMTEMSP
                     IGTHGVIAVKDQAKSFAERMAIKARQGRRVFGVELKIVDENGQRQPHDGEATGELFVR
                     AATVISGYYENAEASAKVFDKDGWFGTGDVAKITPDGYLVIVDRTKDLVKSGGEWISS
                     IDVENAAMATPGVAACAVIGVPHPKWQERPLLVAVKAPGANPTREDVLATLEKAIAKW
                     QLPDDVVFVDSLPLTATGKVSKLTLRKQFAGHKLPDAG"
     gene            32510..33700
                     /locus_tag="PK812_01930"
     CDS             32510..33700
                     /locus_tag="PK812_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRJ68339.1"
                     /translation="MTDLAAFRAETRAFLEANCPVSMRSPVRSDEDICWGGRNWRFQS
                     DDQRLWMERMAARGWTVPTWPKAYGGGGLSHEEAKVLKEEMSAIGARQPLLSFGIWML
                     GPALLAFGNEAQKLEHLPRIARGEIRWCQGYSEPGAGSDLASLQTRAEDKGDHYEVTG
                     QKIWTSYADKADWIFCLVRTNATAPKHLGISFLLFDMTSPGVTTRPITLISGKSPFCE
                     TFFDAVKVPKANLVGTLDRGWDIAKYLLTHEREMIGEGGSGLLGGRSPGQMATDAIGL
                     DATGRLADPFLRADAAMAEVDALAFALTMDRYKDEAASGGRGVGDRSAILKYAGTELN
                     KRNHELMMRAGGHEALEWEGQRSNDGSLPRAWLRTKANSIEGGTSEVMLDIIAKRLLQ
                     LPSA"
     gene            33709..34845
                     /locus_tag="PK812_01935"
     CDS             33709..34845
                     /locus_tag="PK812_01935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRJ68340.1"
                     /translation="MLILNEDQKMLQESARGFLESRSPVSALRKLRDDRSIDGFDRAL
                     WAEMAEMGWAGILVPEEHGGLGFGYVGAGILCEEMGRTLAASPFLSTSVMAATALRAS
                     GTLGHQQRWLPGIAGGRAIIAAAVDEGRVHDPAGTALAAERHGNGFLLRGSKAVVVDG
                     HVADAFIVAARTAGAPGEKDGVTLFLVDAKAKGLSIERTIMLDSRNAASLQFDGVEAT
                     GADILGETDAGYPLLEVMLGAGRAGLAAELAGIAAESFGRTVSYLKERKQFGRTIGSF
                     QALQHRAAHLFSEIEIVGSAVLNALQALDRDANTASLAVSVAKAKAASVARLAAQESV
                     QMHGGIGMTDAVDIGLFMKRMQSASMLFGDVSFHADRVALARGF"
     gene            34916..35377
                     /locus_tag="PK812_01940"
     CDS             34916..35377
                     /locus_tag="PK812_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HRJ68341.1"
                     /translation="MTKQVTFNDMRASVGTEVGVSDWIEITQDMINRFADVTFDHQFI
                     HVDVEKAKLSPFKTTIAHGFLTVSLLSAMAYSGLPDIKGRVMGVNYGFNKLRLVSPVP
                     SGARVRARFTLKAHDEKLPKQHTFTFDVTVEVEGSGKPALVAEWVTLSFEG"
     gene            35389..36318
                     /locus_tag="PK812_01945"
     CDS             35389..36318
                     /locus_tag="PK812_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRJ68342.1"
                     /translation="MSIRFDGRVAIVTGAGNGLGRCHALGLAERGAKVVVVDFGGARD
                     GSGGSSEAARKVVAEIEAKGGEAMAHGANVTDMAQVTDMVEKTMARWGRVDILVNNAG
                     ILRDKTFSKMDLADFKTVVDVHLIGSVNCTKAVWEIMRAQNYGRIVFTSSGSGIYGNF
                     GQSNYGAAKAGVVGLMNVLHQEGSKNDIRVNTLAPTAATRMTEELLPKEALDLMTPES
                     ITPGLLVLVAENAPSKFILSAGCGTFARIVIEETDGVYFPETERTPEQIAARLGEICD
                     TRGKAPLPGAFDQTKKLIAKAAAAKGVSVPGLS"
     gene            36345..37535
                     /locus_tag="PK812_01950"
     CDS             36345..37535
                     /locus_tag="PK812_01950"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HRJ68343.1"
                     /translation="MREAVVVSTARTGLAKSWRGALNNTHGASMGGHVVKHAMQRAKV
                     DPGEVEDVIVGCANPEGATGWNIARQVALAAGCPVSVSGVTVNRFCSSGLQTIALAGQ
                     RIMAGEGDVYAAGGLESISLVQNQMNKHKFMDAGLTKSKPEVYWPMLQTAETVARRYN
                     ISREVQDHYGVESQRRAAAAQQGRKFDDEIVPFTTMMTMAETNEKKEVVRTWEQEVTL
                     KTDEGIRADTTYEGVSKIRPAVEGGVISAGNASQFSDGASMSIVMDSKLAEKRGLAPL
                     GIFRGFAVAGCEPDEMGIGPVYAVPKLLKRHGLTVEDIDLWELNEAFAVQVIYSRDKL
                     GIPNDRLNVNGGAIAVGHPYGMSGARLVGHALIEGRRRKAKYVVVTMCIGGGMGAAGL
                     FEVA"
     gene            complement(37583..38356)
                     /locus_tag="PK812_01955"
     CDS             complement(37583..38356)
                     /locus_tag="PK812_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68344.1"
                     /translation="MRRTWMAAVFVAMLTPVAAQTAQESIRPTGLALLPDTPKGHFGT
                     PLPSTQFNEHQQRKLMLITFDKSAVGQTLNFAVRARSTTASAPAVVFRASATVNEQGL
                     VPIEVKLPRKWPVGHYEVLVFQNDQPVGVLPYRVIADPPRETELSFGDVAIEKVTGPD
                     QVEPVEKVRSSYRHLNFSVKVSGANTNGINLTWVFSAIETTSGSRELHRQDDANRPVE
                     DTELQMDVSLPRDWPIGRYRVDLFVDGKPVVTRGFTIEP"
     gene            38504..39106
                     /locus_tag="PK812_01960"
     CDS             38504..39106
                     /locus_tag="PK812_01960"
                     /inference="COORDINATES: protein motif:HMM:NF037152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRJ68345.1"
                     /translation="MAHRPSLSAEETRGRILEVAEEHFRRVGYAKTAIADIAAVLGMS
                     PANVYRFFASKADIKAAIADKLMSAGRAILRHEAEGAGTAAERLLRVAMLLHRYNKQQ
                     FTADRRLHDMVEAAMSENWTVIQDHLEQITGIFADIIRSGIASGEFALRDPLEAGRTL
                     QQMMASVCHPTLIAQCVDVDLEEQTERIVGYFVRSLKVTG"
     gene            39110..40195
                     /locus_tag="PK812_01965"
     CDS             39110..40195
                     /locus_tag="PK812_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006018944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRJ68346.1"
                     /translation="MRLEAKAILALLLPVLAACGEGTARQKQPSAQPRAVVVEVVRFA
                     PRAPERSLVGVIRPRVETDMGFRVAGKVAERLVQQGERVKAGQPLFRLDPADLNLQRE
                     QAAAELAAARASLASSAAQDQRSGELKKSGWVSQANLDQQLARTADARGRMERAERAM
                     ALAENQAAYAEIRSDVDGIVTATMAEPGQVVAAGAPVVRVARSSGFDAVVAVPELLVD
                     SVRGGRAGVTLWSRPGTTYPATLREFAASADAVTRTFQARFAVSAPEETLAIGMTATV
                     TVVEPDAGKIARLPVSALYSNGRGASLFVVDPADGGLDLRRVTVAGYEGAHVLVTEGI
                     NDGEQVVAFGVQKLDPSQKVRVIEKRG"
     gene            40202..>41781
                     /locus_tag="PK812_01970"
     CDS             40202..>41781
                     /locus_tag="PK812_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRJ68347.1"
                     /translation="MSDVSAFDARKSRGNWSYWAVTHPTLTLFMIMVIMASGMVSFMR
                     LGRSEDPSFTIKVAVITVSWPGATAQEVQQQVAERIEKKLQELPWFDKMQTYTKANFA
                     AMQVQFKDSTPPREVPGLFYQIRKKLDDLKPDLPAGVIGPNVNDEYGDVDSVLYTLTG
                     DGAGYADIKRVAERLKSRLLRVPNVTKVNIYGAQDEKIFVEFSHAKLGTLGVPVQAIF
                     ETLQKQNVLTPAGTIDTGVARIPLRVSGALDGVDAVAAAPVEANGRIFRLGDIATVTR
                     GTVDPPSYLIRQEAVPALAVGVVMQKGANITEFGEALAEAKEQFLAEVPAGIDIHQIA
                     DQPKVVNLAIAEFLRAFAEALAIVLAVSFLTLGLRTGVIVALSVPLVLAVVFSAMYAM
                     GIDLHRISLGALIISLGLLVDDAIIAVEMMVVKMEQGFDRLSAAAHAWTSTAFPMLTG
                     TLVTIIGFLPVGLANSSVGEYAGGIFWVTGLALVVSWFVAVLFTPYLGMKLLPDFRAL
                     ELRKHDRAVRRAARRGRPA"
BASE COUNT         7339 a        13047 c        13596 g         7799 t
ORIGIN      
        1 aaggcgcgcg ccgcgtcata ccagcggccc atggcccccg gcgcgccttc ccgcgcccgc
       61 gccgaaccga acaccacaag cgtcgaggcg atgcggtggg cacgcaggac ctcgtccgcc
      121 ttttcgaatt ccagcatgaa ccgcgcgccg cgcgtgctcg gcccgagcag gaagtcggga
      181 tcaagcatgg cgaggcgata ggtcggcgag gatttctggg cggaattgtc cggcacagcc
      241 tgctccgttc ggaatcgaac gtcagcactt tcgcatggac cgaattctga tagaaccccg
      301 gccttgtctc ttccagccag tgccgccatg accgaccttc ccagcctgca agccgccatt
      361 ctcgccgaca tcgccgcagc aggcgatgaa gcggcgcttg agtccgtccg tgtttcggca
      421 ctgggcaaga aaggctccat atccctgcaa ctcgcaggac tcggcaagct cgctccggag
      481 gatcgaaagt ccgcgggcgc cgccatcaat gcagtcaagg atgccgttgg cgaggcgctg
      541 tccgcccgca agtccgtgct gaaggcagcg gcgctggatg cccgcctcaa ggccgaggcg
      601 ctggacgtga ccctgccggt gcaaccatcg gctctcgaga ccggacgcat ccatccgatt
      661 tctcaggtga tcgacgagct caccgccatt tttgccgatc tcggtttcgc ggtggcggaa
      721 ggtccggaca tcgagacgga cgatctcaac ttcaccaagc tgaacttccc gctcggccac
      781 ccggcgcgcg agatgcacga caccttcttc ttcccgccgg acgccaacgg tgagcgcaag
      841 cttctgcgca cgcacacctc gccggtgcag atccgcacca tgctggccca gaagccgccg
      901 atccgcatca tcgtgccggg ccgcacctac cgctgcgatt cggaccagac ccacacgccg
      961 atgttccatc aggtcgaggg gttggtgatc gacaagaccg ccaatctcgg cgagctgaaa
     1021 tgggtgctgg cggagttctg caaggccttc ttcgaagtgc cgaacgtcac gatgcgcttc
     1081 cgaccctcgt tcttcccctt cacggagccc tcgatggagg tggacatcca gtgtcgccgc
     1141 cagggcgggg aaatccgctt cggcgaggga gaggactggc tggaaatcct gggctgcggc
     1201 atggtgcacc cgaacgtgct ccggaactgc ggcctcgacc ccgacgagta ccagggctac
     1261 gcctggggca tgggcatcga ccgcatcgcc atgctgaaat acggcatgcc tgacctgcgc
     1321 ccgttcttcg acgccgacgt gcgctggctc acgcactacg ggttccgccc gctcgacctg
     1381 ccgacgctgg ccggcggtct gacgggctag ataatgcgct tgcgaatggt ggacgtgacg
     1441 atctcggtcg cgatcaccac aaggaagatg accgccagaa tcaggccgac ctgatcccag
     1501 tagaggttgt tcagggctga atcgagcgca acccctatgc ccccggcgcc gaccagtccg
     1561 agcacggcgc tctcgcgaat gttgatgtcc cagcgcaaca aagcaacgga ccagaaggcc
     1621 ggctcgacct gcggccagta gcccttggcg agaatcatcg agcgcggtgc tcctgccgcc
     1681 gtcagcgcct cgatgggccc acgttgtgcc tcttcaagcg cttcgccgaa caacttgccg
     1741 atgaagccga tggacctgaa ggcgatggac agaacgccgg acaaggcgcc ggggccgaaa
     1801 atcgccacga acagcagcgc ccagatcagc gagttaactg agcgcgagga tacgaagatg
     1861 aactgggcga ggaagttcag cacggggctt ttcgtgaaat tccgtgctgc caggaatgag
     1921 agaggaagcg ccaggacaat ggcgagcatg gtcccgagcg ttgcgatgtg aaaggtctcg
     1981 atcagggcgt catgcaccgc cttgggataa aatccccagt cgaaaggcca catccgtttg
     2041 aacagatcgg ccgtctgctc gggagcgtcg tagagaaact ccgggatgat atcgatcgtc
     2101 ttgatcgacc agaccacggc gacggcggtg acgattgcca acatcgagcg cgcaattcgc
     2161 tcggcgggac tgaaccgctg ccatctgcgt gcctgatccg gcggcgcctg tgtttccttc
     2221 actggaagat cctccggatt cggccggaga caacttctgc cagcatgatc aatgcgatga
     2281 tgcagatcag gatggtcagc acgacatcgt agtcgaagcg ctgataggcg gtgaacagcg
     2341 gcgcaccgat tcctcccccg ccaacgatgc ccaccatcgt ggagttccgc aggttggagt
     2401 cgagctggta tacggacagg ccaacgaaac gggccatcac ctgcggcatg accgcgaaga
     2461 tcagcacgtt ccagaagttc gcgcccgtgg cccgcaccgc ttccacctgc ttggcgttca
     2521 tctcctcgac cacttcagcg aagagtttcg agacaaagcc catggaagag acgatcaacg
     2581 ccaggatgcc ggccagcgcg ccgaacccga ccgccttcac gaacaggatc gaaacgatca
     2641 cgggatggaa ggagcgcagc acggcgatga gtccgcgcgc actccagctc aaccagcctg
     2701 gcgcaaggtt acgggcggcc atcaggccga ggggcaggga caggagcacg ccgaagaagg
     2761 tcgcaagaac cgcgatctgg atcgactcca gcatgcctga gccgatcagt tccagcttgt
     2821 ccgcctgcat gttgggcggg aacatgcggt caaggaattt cgcgccgttg tcgaggccga
     2881 cgacaaagcg cgcccaggta aggttcagga tcgacgtggc atagagcgca tagagcgcaa
     2941 gtccgatcca ggcgagccgg gccggccagt tgacgggaaa cgcaggaggg cgtggacggg
     3001 atgccgcaag gctcacaaaa agtcctcgcc gccgtagatg tgcttgagat gatcgtcccg
     3061 caacccgtcg ggtggcccgt catagaccac ttcgcctccg gtcatgccaa tgatgcggcc
     3121 ggcaaaccgt ttcgccagac ccacattgtg gatgttgacg atgatcggaa cgccccgctt
     3181 gtcggacagc tcggccagca gttccatgat ttcaactgat gttttcggat cgagcgacga
     3241 tgtcggctcg tcggccagca ccaacgccgg atcctgcatg attgcacggg cgatgccgac
     3301 gcgttgacgc tgccccccgg agagagcgtc ggcgcgccgg gtggcgaagt cctggttgag
     3361 accgacggcg tcgatgagat caaaggcacg cgcgatgtct gcctgcggga accggcgcag
     3421 gaaggcgcgc catgccggga catagccaag ccggcccgac aacacgttct cgatcactga
     3481 caggcgttcc acgagattgt actcttggaa caccatgccg atgcggcgcc gcgcggctcg
     3541 aaggggggct cccgagagct ttgccaggtc ctggcccgcg aacaggatct ctcccgaggt
     3601 ggggtccacc agccggttga tgcagcggat cagggtcgac ttgccagtgc cggaaggacc
     3661 gatgatggcg gtcactccct tggcgtctat atcaaggctg atatctttca ggacaggctg
     3721 gccgagacga tactccttcc gcaggttgcg gatggtcagc agcgccgaat tcattgcttg
     3781 ggcgccgccg gctgtcctgc ggcggccttg gcggctgctt ctgctgcctt tttggcggca
     3841 gcatcagcct cgcgctttgt ctgcgcatcg taggcggccc tgttgtacgg cgttcccgac
     3901 ttttcggcga cgtcccggat gggccgccag gtgtccttgt aagtcatcgg caggaaccgg
     3961 tcctctccgg cgtattcctt cttcagtccc tcatgccact tatagtccat gaagcaggcc
     4021 gtgatccttt tcgccagttc aggcttcagg tcgtgcggaa tggaccagga agacgtcggg
     4081 aacgtctcgg attgccagat cacgcgcagg gcatcagcct tgacggcgcc gcgcgccacc
     4141 atgcgctcat agacgtccga ggcaaccggg gccatgtcgt agtcaccgga ggcgacgccg
     4201 agcaccgact tgtcgtgccc gcccgacatc aggaccttgt aatcctcctc cggcttcagg
     4261 ccattgtccg ggaagaacac gcgtggcgcg agattgcccg aattcgacga cggcgaggta
     4321 tgcgcgacct tcttgccctt gaggtcggaa agcgtcttgt aagggctgtc ggccttgacc
     4381 agggcaaaga gcttgtagcc cgaaacgccc ttgtcggacc cgatgacagc gaagggcaca
     4441 gcgcccgcga gattgacggc gaatccaacg acgcccgtgg cgtaaccgga aatgtgcagt
     4501 cgccccgagc gcattgcctc gatctgcgcc gcgttgttct gaaccgggaa gtagacgatc
     4561 cgcttgccgg tgcagcttcc gagatagtcc atgaaaggct ggtagaccgt ctggtagacc
     4621 gcaggatttt ccaccggcga gtacgcgaag atgagcgttg ccggatcctt gtggcgcgac
     4681 ggatcggtcg gcaggtccgc aacgaggtcg ttgttggcgt cacaataggc catatccagt
     4741 tgcccgcgat tggggcagga atcctgggca agcgccggga aggcggcagc caccgccgca
     4801 gctacaatca tgttccggaa cattgaacgg ctcactgttt gggaggtgtc gtggtggctg
     4861 gctgtgcggc ctttgcggca gcctccgcag ccttcttggc tgcggcgtcg gcttcgcgct
     4921 tggcttccgc gtcgtaggca gccttgttgt agggcgtgcc cgatttctcg gcaacgtcgc
     4981 gcaccacttt ccagtccttg gcgtaggcga tcggcaggaa gcggtcgtcg ccgttgaact
     5041 ccttggtcat cgctggtgtg aagcggaagg aatagaagca ctccttcagc ttggaggcga
     5101 gttcgggctt gagatcgtgg gcatgggcga acgatgatgt cgggaatacc ggcgagcggt
     5161 agatgacgcg gaagtcatcg cccttcactg tgccccgcgt caccatgcga tcgaacacgt
     5221 ctgaagcgac ggcagccatg tcgtagtcgc cggagttgac gccaagcacc gacttgtcgt
     5281 gtccgcccga catcagcggc ttgtagtcgt cgtcggtctt caacccctgc tcggggaaca
     5341 gcacgcgtgg cgccagattg ccggagttgg aggatggcga agtatgggcg accttcttgc
     5401 ccttgaggtc cgcgagggac ttgtaggggc tggatgcctt gacgatggcg atcaggttgt
     5461 agccgcgcac ctcgttgccg agacctttcg cggcgaaggg cacggcgcca gccagattga
     5521 cggcaaaccc cgtcggtcct gtggaaaatc cggcgaaatg aagtcgtccg gaccgcatgg
     5581 cctcgatttc cgccgaattg gactggacgg ggtagtagac agtccgcttg ccggtgcatt
     5641 tctcgagatg gtcggtgaag ggtttgaaga tgttggcata aacagccggg tcttcaaccg
     5701 gcgtataggc ccagaccagt gtggaggggt cgcgccattt cttcgggtcg gccggggcgt
     5761 cagcgacaag gtcgttgttc gcatcgcagt agagcgtgtc gagctgtccc cgattcgcgc
     5821 aggcgtcctg cgccaatgcc gttaccgaaa gccccgccag tgcggtcgcg gtcaggaacg
     5881 cacggatcac gagcgactgt tgcatggtat cctccccact cggcgctgac gccgaattgt
     5941 tcgttggcta ccaatcatgc cgagcccggc agctaagtca acgcctgtgg gtggcctgcc
     6001 ttgtcagtcg gcatgacagc accgcggcag ggcactcaat ctgcctttga cagacgccgg
     6061 tgttggcttc atcgttgagg aaatcaaacg ttctcgggag gcattagatc atgatcagcc
     6121 gcagagtcgc acttgcagcc gccgcactgt cggtagcgct tgctggcgca gcccatggtc
     6181 aggccccctc cggcaaggtc accgtggtga cgtccttttc gaaggaggtc acggatccct
     6241 tcaaggcggc gttcgagaag gcgtttccag ggacgactct cgaagtccag aaccggaaca
     6301 cgaacgccgg cgtcaagttc atcgaggaaa ccaagtccaa caacggcgtc gacctgttct
     6361 gggcttccgc gccggacgcg ttcgaggtgc tcaaggccaa gggtctgctg gaggcctaca
     6421 agccgaaggc ggacggcatt ccggacaaga tcggccattt cccgatcaac gatccgcaag
     6481 gcttctattc gggctttgcg gcttccggct acggcatcat gtggaacgat cggtatgtga
     6541 aggccaacaa gttgcctgag ccgaaggagt gggaggatct cgcgacggcg ccgtacttcg
     6601 accatgtctc gatggccgcg ccctcgcgct cgggcacaac gcatctgacg atcgaggcga
     6661 tcctccagtc agcaggctgg gaaaagggat ggggcatcgt caaggccttc tcggggaacc
     6721 tgcgcggcgt caccgaacgg tccttcggcg tgcctgatgc ggtcaattcc ggccaggtcg
     6781 gctatggcat tgtcatcgac ttcttcggct tctcggcaca gggcgcgggt tttccggtca
     6841 agtttgtcta cccctctttg tcaacgatcg ttccggcgaa tatcggcgtg gtgaagaacg
     6901 cgccgaacgc ggcgggagcc aaggcgtttg tcgacttcat cctgtcggag ccggggcagg
     6961 ccattctgtt cgaccccaag atccgtcgac tgccggtacg caccgcgtcc tacgccaagg
     7021 cccccgccgg cctgccgaat ccgttcgaat tcaagtggct tgccgatccg aagtcgaaat
     7081 tcgacgtcga aaaatcggaa aagcgcttcg ccgccgtcga tgcgctctac gatcagcttg
     7141 tcaccttcca gcacgaggcg ctgaaagcgg cgaccaaggc cattcatgac gccgatgctg
     7201 ccatcgcgcg caaacccagt ccggaggcat cgcgccttgt cgcggaagcg cgcgccttgg
     7261 ttgcccggat gcctgtgagc gaagcggagg cggcaagccc ggaaatggcg gctgctttca
     7321 caggcggcaa ggagaaaggg ccgcgccagg cggaactcga gcagaaatgg gcggcttacg
     7381 ccaaggagaa ttacgcggca gcgctggcga aggcgcaggc tgcccagcag gcggcaaagt
     7441 gacggcggct ctccgcgcgc aacggacgga tgcactcgac tgccagacgt ggagcgggga
     7501 ggccggctag tgtcaggcag cgcttttcct gtccagggcg cggggcgcgg gattgcttcg
     7561 cccggcctgg cgctgttggc cctgctcatc gggttgtttc ttgccgcctt tctggtgatc
     7621 ccggttgtta cggtcatttt cgtcgccttc cagcccaagg gatctactgg cttcacgctg
     7681 atcaatttcg tcgatttctt ctcgaccgat ctgtttcggc ggtcgttctg gaactctgcc
     7741 tacgtctcgt tgatgtcggt ggtgatcgct tcgctgctag ccctgccgct cgcctactac
     7801 acgacacggt tcgagtttcg gggcgccgca ttggtgcaga cgctcggcgt cattccgctg
     7861 atcatgccgc ccttcgccgg tgcggtggcg atgcagctcc tgttcgggcg caacggcacc
     7921 gtcaatcttc tgctcgacga ttggttcggc ttcaaggttc cgttcatgga ggggttgaac
     7981 ggcgtcatct tcgtggaagc ggtgcactat ttccccttca tccttgtcaa tctgtcgacg
     8041 tcgctgcgca atatcgaccg cgccatggaa gaggcggcgc aaaacctggg ctgctccggt
     8101 tttcggctgt ttcgccgtat cgtctttccg ctcgccatgc cgggctatgt tgccggcgcc
     8161 tcgctggtat tcatcaaggt gttcgacgac ctggcgacgc cgctcctgct caacgtcaag
     8221 gacatgctcg caccgcaggc ctacctgcgc atcacctcgg tcggcctcgc cgatccgatg
     8281 ggttatgtca tctcggttgt cctgatcgtc gcttcgttgc tggctatgtg gctgtcgacc
     8341 cttgcactca ggggaaagga ctacgctacc gtgcaacgcg gcggtggcgg aacagtgaag
     8401 cggccgctcc gtggcatcga ccgcgtgctc gcctatggcg ccattacggt ggtgctggtg
     8461 ctggtgctgg caccgcatgt aggcctgttc ctgctttcga tcgcgacgat ctggtcattc
     8521 gctcccttgc cggacgggta caccgctgcg cactatgcaa gggtgctttc cgagagcgga
     8581 cagtacatca ccaatacgct gatctatgcc ggtctcgcgg gactgatcga cgtggtgatt
     8641 ggctcggcca tcgcgtatct cgtgctgcgc accggcatgc cggccggccg ctggcttgat
     8701 tatgtcgcga cggctgcgct ggccgtgccg ggcgtcgtac tgggcattgg cttcctgcgc
     8761 acgtatttcg gggtcacctt gccctccggc cagtcgctgg cggccttctg gggcatgatc
     8821 gtcctggcgc tggctatccg ccggctgccc tatgcgctgc gcgcctgcat ggcggcgatg
     8881 cagcagatct cagtcagtct ggaggaagca gcgcaaaacc tcggcgcgac acggtcgcgt
     8941 acggtgcgcc gcatcgtcat gccgctcatg ctctccggcg tggctgcagg gttcgtcacc
     9001 agtttcgcga cggcggccgt tgaattgtcc gccacgatga tgctggtgca gacccaatcg
     9061 gatgccccgc tcgcgtacgg tctttatgta ttcatgcagt cgccggccgg gcgtggcgca
     9121 ggtgccgcgc ttggcgtcat cgccgtggtg atcgtaggcc tgtccaccta cgtttccaat
     9181 cgcctggttg aacgcgacat ggcggccaga tcggaggcga aggaatgact gcgctacttc
     9241 agcccgtcgg tatcgatgtt gaaggcgttg accttgcttt tggcgcgacc caggtgcttc
     9301 gcaatgtcag tctatcggta cacccgggcg agttcttcgc ctttcttggt ccatccggct
     9361 gcggcaagac cacgctgctt cgtttgattg ccggcttcaa cgtcgcccag aaggggcgcg
     9421 tgctgatcgg tgggcgtgat gtcacggcgc tgcctccctg gcgccgggat gtcggcatgg
     9481 tgtttcagag ctatgcgctg tggccccaca tgagcgttgc ccgcaatgtc gccttcgggc
     9541 tcgaggagcg gcgactgccg cgctctgaag tcgccgggcg cgtcgatgag gcgctcgatc
     9601 tggtcgggct gcgtcacctg aaggatcgca agccgtcgca gctgtccggc gggcagcagc
     9661 agcgcgtggc gttggcccgc accatcgccg ttcgaccgca ggtgctcttg ctcgacgagc
     9721 ctttgtccaa tctggacgcc aagatgcgtg tcgaggtgcg gcgggaattg cgcgagctcc
     9781 agcaaaggct gaagcttacg actattttcg tgacgcacga tcaggaggag gccaatacaa
     9841 tctgcgaccg catcgcggtc atgagcgaag ggatcgtcca gcaggtgggt gcgccgatgg
     9901 acctctacga ccggccggcc aacctgttcg tcgccggctt tctgggaacg gcaaattttc
     9961 tagaggaagg cggccgaaag gtcatggttc gcccgcagaa tgtgcagctg ggtgccgaag
    10021 ccgcgacagg cgctttcatg atctcgggcg ccatcgtcca ccgcgaattc cagggtgcga
    10081 ccatgcgcta tgtccttgag acagagcggg ggccgctgat cgtcgacgag ccttatgatc
    10141 cgggccgatc tccctacgcc gttggatcac aggtgaacgc cgcgatccgt cctgagcgcc
    10201 ttgtggcggt tgcctgattg ggcgctccgc agagcacgaa aacatcgtgt gatctctcgg
    10261 ccagaccggg cgtactgacg ttcgagcgaa gtaccgcatt cagagaagag tggatcaacg
    10321 cgtcgaaaca cccgtcacct caagtgcgag ttcgaaggtc atgaggaccg ggttggcgcc
    10381 tctttgcagg cggccccgct caacgcgacc gctgaaatcg atcaacccca cgtcgatggt
    10441 ggcgacaggc aggccatttg tccctggagc aatgctgccg cgttcgatga acacctcggt
    10501 ggcgaaattc gtgacctcac gtccgtcatc gaaaagcgcg ccgatggtcg agccgacgcc
    10561 gccccgaatg cgtgcatgcg ctatggcatg ccgcacacag tagtcttcga tggcacggtg
    10621 caggtcgaca ttggggtgta cacgcaaggc atgggcggtt cgatcatgac caggagccga
    10681 tgcagccgcc ggccccggca cggtgaaatt gatttcggga tcaaggcgcc atgtgaaggt
    10741 ggcgccggtc agggccagcg cctcgacctg tgccggctcg gccagcagcg tttcctccgg
    10801 caggagatgg cctccgcctt gctttccgtc ctgctcgcgc caaagggcgt gggcatgaaa
    10861 gaaggggctc tggtcccgta gcccgaacgt catcgccccg gccgccagcc gcgtcatgcc
    10921 agccgggcgg aagaccgggc tgtagaaggc tgcatgccga ccgttttctg ccagcgcggg
    10981 catgacgtaa ccgaacggtc caagcgcgag atcccggaga tcgacgacgc cggaatcatg
    11041 ccccgtcctg gcaagagcgc gccgcacggc ttcgagcagc ggcaaccctg ccggcaggtc
    11101 aagcgtgagc gctgttccct gcgccggcgc cgccgaaacg cgcgccgcga tggccgcacc
    11161 aggttgtcga acatagccgg ggcgtgccac gtcagatcct gatcaactgt tccggcgtca
    11221 gcaggccgcg ggcttcgagt tcgtcacgca ccatgcgttt gggtaccttg ccgtatccgg
    11281 attttggcag ggcctcgaaa aagaacaccc gcttgggcat cttgtagcgc gtgacctttg
    11341 gcgtgaggaa ggcgacgatc tcgtcctcgg tcacgcactg accgtcacgg agcacgcaga
    11401 ccgctatgcc cgtctcgccc cagagcgggt cgggcacgcc gacaatggcg acttccgcaa
    11461 tggccggatg catcaggatc ttttcctcga tctcgcgcgg atagacattg gagccgccgg
    11521 atatgtacat gtccgaggcc cggcccgtga tgtagacata gccctgttcg tccaggtgac
    11581 cgaggtcgcc ggtgcggaac cagccgtcgc ggaatgcctt ggcattggct tcgggattat
    11641 cgtagtagcc ggcaaagacg gccggaccaa tcacgcagat ttcgccagtc tcgaatggct
    11701 tgagttcccg ccctgcgtcg tcctggatcg agacctgcat cccggtgcgc tcgaaaccgc
    11761 aggtgccgat acgtgccgcc ggtccatccg cttcgtcgtg cagccgggcg ggcaggaagg
    11821 taatgttgcc cgtgacttcg cccaacccga agtattgcac gatgcatttg ccgagcgcgc
    11881 gcagcgccgt cttctgatcc tcccggtaca tcggagcgcc tgcgtagatc acgtgtttga
    11941 gcgacgaatg atcataggtc cgggcggccg gatgctccgc cagcatcttg aggatggtcg
    12001 gcaccgtgaa catgttggtg atccggtgct cctggatcag ccggaaggcc tccggaatgt
    12061 cgaagcgttc gctgggcagc aggatcgtcg gggcgccacg tgccgcaatg tcgagttgat
    12121 gaacaccggc tccatggctg agcggcgcga caaccagcga ggcatcgtcc tcggtagcgc
    12181 ccggcaggag gtcagcaagg tggtttgtga tgacgaaggc catctggcca tgggtcagga
    12241 cggccgcctt ggaacggccg gtggtgccgg acgtgaagaa gaaccagcag ggatcgtcgt
    12301 ggtcgaccgg agcattctcg agcacggctc cgccatgccg cgcgatggcc gcgcccacgg
    12361 tcgtttcgcc gaagctggca gagccaccca tacgccagat gaagtcgacg ttagcggccc
    12421 ggtgggcgat catggcggca tggtcgggaa agtcgccgtg gcagaggaat gccttggcgc
    12481 ccgagaaggt cgcgagatag acgatttcct ccggcagcag acgaaagttg gtcgggaccc
    12541 agaccgcgcc gagccggaag caggcgaaca tcgagaagta catctcggcg cagttcttcg
    12601 aatgcaccag cacccggtcg cctttggcga taccgcgggc cgccagcgcg ctcgcaagcg
    12661 ccgtcaccgc cccgtcaatg gcgtgccagg accagctttc atccccatgg atcaggcctc
    12721 gatggtccgg cagccggcga cggttctggg tgaggatatg ggcgaggttc atgacacgcc
    12781 gcgagcatcg cggaacgccg cccgcaggag cgctaccctt ggtcatgcgc aggattccga
    12841 aggttctagg gagccaggac gctggcgcct ggcgccatca tggcaaagcc atggtgcacg
    12901 ggcaatgggc gattcagctc ccccgcgccg ccgccttttg cgcaccttcg acaatcaggt
    12961 cggtagccag gtccgcgtag gcctcgcggg tgatcggtcc atcctcgcga aaccacatgt
    13021 aatgccagtt cagcatgccg aaaagactca tggtgacagg cttgatcagt gccgatcgtt
    13081 tctccagcga cggcactgcg gctgcgaccg ctgcggaaaa cctgcgcacc agaacccttt
    13141 cgagttcaac cagttcctcg gcccgaggct tcgggagctt cttcaattcg ttgatctgga
    13201 tctggtgctc atggtcagcg tcgcgatagc agtcgagcaa ggcgccaacc agtgcgcgga
    13261 gccgtgcgcg gggcgagccg ccagcgggcg gcagtgtcgc cgccaccaac tcttccagat
    13321 gctcgcgaat gatggccacg agcagctgct ccttggtgtc gtaataatgg tacaggagcg
    13381 ccttcgagat ggagcattcc gccgcgatat ccgccatcga agcccggtca tagccatccc
    13441 gcgcaaacag gcgcgcgctt gccttcagaa tggccttgcg cttgtcaccg tagtcggcgg
    13501 ccaatggtcg cgccatcgcg tcagaccttc gggcgattgt cgatgatgcg acgcgccttg
    13561 ccggcggagc gctcgacagc accaggatcg ccaacgttga tcctggttga aacaccgatc
    13621 gtcgacttga tcatgtgagc aagccgcgcc gccgctgcct tgcgatcctg atccgtcacg
    13681 gagccgtcgc gcgcctccag cttgatctcc atctgatcca tgcggccttc gcgcgtcagc
    13741 acgatctggt aatgggccga caattcggga attgcgaaaa gcttttcctc gatctggctc
    13801 gggaagacgt tgacgccgcg caggatcatc atgtcgtcgg agcgacctgt caccttttcc
    13861 atgcgccgca tggagcgcgc tgtgccgggc agcagacgcg tgaggtcgcg cgtgcggtag
    13921 cggataaccg gcatggcctc cttgctgagg gaggtgaaga caagttcgcc catctcgccg
    13981 tccgggagca cctgaccggt ctcgggatcg atgatctcgg gatagaagtg atcttcccag
    14041 atgtgcagcc cgtccttgct ctcgacgcat tcgttggcga cgccggggcc catcacctcg
    14101 gacaggccga agatgtcgac cgcgtccatg tcgaaggcct gctcgatctc ctgccgcatg
    14161 gcattcgtcc atggctcggc gccaaagata ccgacctgca gggaggtcgc gcgcgggtcg
    14221 atcccgacgc gccggaactc gtcgaggatc gccagcatgt agctcggcgt caccatgatg
    14281 atctcaggct tgaagtcggc gatgagttgc acctgccgct cggtcatgcc ccccgagatc
    14341 ggcacgacgg tgcagcccag cttttcggcg ccgtaatgtg cgccgagccc gccggtgaac
    14401 aggccgtagc cataggcgac atgcaccatc atgccgggcc gacctccaga agcgcggatc
    14461 gagcgcgcca tcacgtcgga ccaaacggcg atatcgccga gcgtgtagcc gaccactgtc
    14521 ggcttgccgg tcgttcccga tgaggcatgg acgcgggcca ccttatcccg cgggacggca
    14581 aacattccga aggggtagtt gtcgcgcaga tccgccttgg tggtgaaggg aaacctggcg
    14641 aggtccgaga gggacttcat atcgtcaggg tgcacgcctg ccgcctcgaa cttcttccga
    14701 taggccggca cgttgtcgta ggcatgcctc agcgaccatt tcagtcgctg caactgcaat
    14761 ccggtgatct cgtcccgcga cgcgacctca attgggtcga ccatgggctt gtagctgttc
    14821 ttgatgatca tgctcccctc cctgatcccc tcaggaaacg atgatgacgc cggggaaaaa
    14881 ctcccctttt atggtgcgcg agttgccacg aaacacggca atcgttgccc ccgcctggtt
    14941 ctgcaccgcg atgtcataga tgcccgaccg ccctgtgcgc gagacctcgg tgccggtcgc
    15001 ggtcagcaca tcgcccagtc ttgccgcggc gatgtaggta atggcgcagc tctgcgcgac
    15061 cgtgcgggca ttgtaggtgt tgcaggcgaa ggcgaaggcg gaatcggcca gggtgaagat
    15121 gtagccgccg tggcagatgc cgtgtccgtt caccatcgtc tcggtgacgg gcatggtcag
    15181 gacggcgcgg ccagcggaca cggcgccgag tgccatgccg agggttcggc tggccttgtc
    15241 ttccgcccac atggcgtcgg cgcaggccct ggccagttcg ttcgcagtca tggattgcat
    15301 ggtaacctca aggacgtccg gtgaagttcg gcttgcgctt gtccatgaac gcgcgcacgc
    15361 cctcggcata gtcggcggaa acccccgcac gccgctgggt atcgcgttcc agatcgagct
    15421 gctgatcaag cgtgtttgcg tgggaggcct gcaaggcccg ctttgccagt ccgatgccat
    15481 aggtcggagc ttgggcgagg cgagcgcaca gcgcatgtgc gtcggccatc aattgcgcgt
    15541 catccacggc tttccagatc atgccgatgc gttcagcttc ttccgccttc acaggttcgg
    15601 ccagcagcac cagggctttg gcgcgtgcct cgccaaccag cctgggcagg aaccaggtgc
    15661 ccccggaatc gggtaccagg ccgatcctgg aaaaagcctg cagaaagctc gccgagcgcg
    15721 ccgcaagcac gatgtcgcag gccagcgcga tgttggcgcc ggcaccggcc gcgatgccgt
    15781 tgacggcgca gacgaccggc ttgtccatgc cgcgtatggc gcgcacgagc ggattataaa
    15841 aagcctcgat ggtccgcccc agatcgggtg catcgccacc ctcgccctgg acacgatcgg
    15901 acaggtcctg gcccgcgcaa aacccgcgac ccgcaccggt cagcagcacg gcccggcatg
    15961 cgggatccgc agcggcgtga tcgacagccg actttagcag acggtgttgt gcctcattga
    16021 aggaattgag ccgatccgga cgattcaggg tcagcacgcg gtagcccgcg caatcctcta
    16081 ccaggaccgg atcagtcatg tcgtcctccc tatcaggccg tcgcagggca gccggtgttt
    16141 gacgccaaat tagacttgac tgaccggtca gtcaatgaat aatgcacaac gctgcgagga
    16201 ggcctgtccg ctaggagacg gtgcccgcgc gacagggagg gtttgcaatg ccggctttgt
    16261 tcgaaaagca tcgtccgctc ttggaacagg caattgctgc cgcgcgttca cgcgcaaact
    16321 ggtcagccta tccggaatcc cagtcgccca agatctatgg cgaagacgcc aaggccaatg
    16381 cagacaatgc cttcgaagcc ctgaagggca aaccgttcga ccttcccggc caccccggtg
    16441 ggaagcatgt gggcggcgaa gtctcgccct acggctttgc cctgggcgtt acctaccctc
    16501 aggcggaagt ggacgtcctc gtcgcggctt cgaaggcggc gggcgccaag tggggcgccg
    16561 ccagcccgga agagcgggcc ggggttcttc tggagatcat tgcccgcatc aacagggcgt
    16621 cgttcctgat ggcgcatgcg gtgatgcata cgtctggtca ggcttactcc atggccttcc
    16681 aggctggcgg cccgcatgcg caggaccgcg cactggaggc tgtcgcctac gctttcgacg
    16741 agatgagccg ggtgccgggc gccgtgcgtt ggtcaaagca gaccggcaag gaccaggtca
    16801 ccacgctgga caagaccttc cgaatcgtgc cgcgcggcgt ggcgctggtc atcggctgcg
    16861 ccacgtttcc gacctggaac acctatcccg gcatgtttgc ttcgcttgcg accggcaata
    16921 cggtcatcgt caagccgcat ccaggagcga tcctgccgct ggccctgacg ctagggattg
    16981 ctcgcgaggc attggcggaa gccggctttg atccgaacgt ggtcctgctg gctcccgata
    17041 cgattgatcg gccgctgacc aaggaattgg cgctgcatcc cggcatcggc atcattgact
    17101 tcaccggatc gccggtattc ggagcctggg tgcgtgaaaa cgcgaagggc aagctggtct
    17161 ataccgagga ggcgggtctc aattccatcg tcatcgcctc gaccgacaac ttcagggcga
    17221 tggcgacgaa catcgccttc tccctgacac tctattccgg gcagatgtgc acggcgccgc
    17281 agaacatctt cgttccggct ggaggcatcg aaacggatca gggccacaag agcttcgatg
    17341 aggtggcgga cgcgatcaag tccggtatcg acaagctgct ggccgatccc gatcgtgcgt
    17401 cggcgtttct cggcgcggta cagagcgagg cgacggctca gcgggtgaaa gaggccgcga
    17461 aactcggccg tgtcgtgcgg tcatcggaca gcccggttta ttccgccgcg ccaggcgcgc
    17521 ggaccgcgag cccgatcatt cttgctgttg atgcggcaga cgagccggct tacatggccg
    17581 aacgcttcgg gccgatttcg tttgtcatcg ccgtcaggga cgggggcgac ggcatcgcga
    17641 gggctgccgc ggccgccagg caacatggcg cgatctcggc cgcgctttac gccacggacg
    17701 aggccaccat tgacagggcg atcggtgcat tcgccgaggc tggcgtgacc ttgtcaatca
    17761 acctcactgg cggcgtcttc gtgaaccaga acgccgcgtt ctcggacttc cacgtcacgg
    17821 gcgccaatcc tgcgggcaac gcctgcctga cggacgcggc gttcgtgtcg aaccgttttc
    17881 gtgttgtcgg cacaaaacgg caagtcgccg cctgaatttc atggcgggaa cgccccgcgc
    17941 atgtttgact gaaaagaagc cctaagaagg aggaattcat gaccaggttg aaaagactca
    18001 tcggggtggg tgtggttgct tccaccgcca tcctcgccgc cgccgcggcg caggcgcaga
    18061 tcaagattgg cgccgtcttg tcagtgaccg gccccgcctc gttcctcgga gacccggaaa
    18121 aacggaccct cgatatctat gttgagcaga tcaacgcggc cggcggcatc aacggccaga
    18181 agatcgaaat gaaggtctat gacgatgccg gcgatgcaaa tgccgcgcgc accttcgcca
    18241 cgcgccttgt ggaagaagac aaggtcatgg ctgtgatcgg cggttccacc acgggcacca
    18301 ccatggccat gatgccggtg tttgaagatg ccaaggtgcc gttcatctcg cttgccggcg
    18361 cagttcagat catccagccg gttcgcaagt tcactttcaa gacgccccac accgatacca
    18421 tggcctgcga gaagatcttc caggatctga aggcgcgcaa tctgacgacg attgccatga
    18481 tctccggcac cgacggattc ggcgcctcga tgaaggcgca gtgcgaggcg gttgctggca
    18541 agtccggcat caccatcgcg caccaggaga gcttcggtcc gcgagacacc gacatgacgc
    18601 cgcagctgac caacatcaag ggcaaggccg gcgtgcaggc ggtcgtcgtt cccggcttcg
    18661 gccagggacc ggctattgtc acccgcaact atcgtcagct cgacatcaag ctgccactct
    18721 accattcaca cggcgtggcc tcgaagcagt tcattgatct ctccggaccc gctgccgagg
    18781 gtgttcgcct tccggctgcc gccctgctgg tagcggaaaa gctgccggac tccgatccgc
    18841 agaagaaggt cgtggttgac tacaaggcca cgtatgaaaa gcgcaccggc cagcccgttt
    18901 ccacctttgg gggccatgcc tacgacgggc tcatgattct cgtcgatgcg atcaagcgtg
    18961 cggggtcggc caatgctgaa aaggtccgtg acgcgattga gtcgacgaag ggctatgttg
    19021 gcaccggcgg gatcgtgaac atgtcgccga ccgaccacat gggcctcgat ctctcagcgt
    19081 tccgcatgct ggaggttagc cggggcgagt ggactctggt tcagtaggaa ccaggtcgct
    19141 atcggcgcga cggcgaggac aagacaggca ccccggaccg gtccggggtg cctgcaagcc
    19201 ctgcgcctcg agccgcgcag ttgccgcccg gccgcgtgag ggattgccag cctgagcgac
    19261 tgcctgccct ccgtgttcgc caaactccgg accagcgcca tgcctgaact gatgcagttc
    19321 gttttctctg gactgaccgt gggcgcggtc tacgcgctgg tcgcgcttgg gttcacgctg
    19381 atctacaacg cttcggacgt ggtgaatttc gcccagggcg aattcgtgat gctcggcggc
    19441 atgatcaccg tgttcctcgg gcttgccggt ctctggctgc cgctggctgc catgctggcc
    19501 attattctta cggttttcgt cggtctggcg ctctacaagc ttgccattga accggcgcgc
    19561 ggcgctgccg ccgtgacgct gatcatcatc accatcggcg cctcgatttt cctgcgtggc
    19621 attgcgcaga tcgttttcga caagcgcttc cacgccctgc cgcactggtt cggacagcaa
    19681 cccctgaaat tcggcggtgc gtccatcctg ccgcagagcc ttgtcgttct ggcgggcgca
    19741 ctggtcatcg tcgtgctgct ctatgggttc ttcgaacgta ccctgttcgg caaggccgtg
    19801 ctcgccacgg ccgccaaccg gctggccgcg cggctcgttg ggataaatac cgacgtggtc
    19861 gtcggctttt cctttgcggt atcggccgcc atcggtgcga ttgccgggct gcttgtcgcc
    19921 ccgatcacgc tcacgagcta tgatgtcgga accttgctgg cgcttaaggg gttcgccgcc
    19981 gccatgctgg ggggcatggg caatccggtc ggggccgtcg tcggtggtct tgttgtcggc
    20041 ctcctggaat cgctggccgc cgggctgatt tcctcgaaat acaaggacgc catcggcttt
    20101 atcgtcatat tgttcgtgct cttcgcgatg cccaacggac tgttgggcaa aacgaaagtg
    20161 gagcgcgtgt aatgcagcgg ttcctttccc atcgcctcac cgtcgttgtc gtgctggcgc
    20221 tgattgtagc gctgctgccg ctggtgtttc catccgccta ctactaccgc gtcgccgggc
    20281 aggtcttcgt cttcgggctt gccgccatcg ggctgaacct gctgatgggc tatgccggtc
    20341 aggtcagtct cgggcatgcc ggattcatgg gcatcggcgc ctatgcggca gcgatcggac
    20401 ccgcacagac cgggacacct gtctggttct gggtggtggc cggctcggcg ttgtccggcc
    20461 tgatcgcctg ggttgtcggg cggccgatcc tgaagttgcg tggccactac ctggcggtgg
    20521 cgaccctggg ctttggcatc ctccttttca tggtcttcac caacgaatcg cgctggaccg
    20581 gagggccgga tgggatgccg gtgcaaaggc tcgaactctt cggctggcgc gtccgaaccg
    20641 gcatcgagtg gtactgggtc agcggcgtgc tcctggtgct tggcgcctgg gttgccggca
    20701 atctggtgga cagccccacc ggccgcgcca tgcgcgcgat ccacgacagc gaggtcgctt
    20761 ctcgatctgc cggcatcgac gtggccagac aaaaactcac ggccttcgtg atatccgccg
    20821 tctatgcctc tgtcgccggt gcttcctttg cgctgatgaa cgggcatctg acgccggatg
    20881 tgtcctcgtt ccttcgctcg gtggaacttg tgacgatggt cgtggttggc ggcatgggct
    20941 cgatcttcgg ctcgctggtc gggtcggccg tgcttgtcgt tctgccgcag gcgctcacgg
    21001 tgtttcatga ctacgaacac gccattcttg gcctcatgat catgctgttc atgatcttcc
    21061 tgcgggaagg catcgtgcca tccctggccc ggcgttttgg ccggaggagc gcatgagcaa
    21121 gctgctttcc gtttccgacc tgtcgatttc tttcggcggc gtgcaggccg tggatggcgt
    21181 ttcgttcgag gcgcggaccg gcgaaatcta tgcgatcatc ggaccgaacg gagcgggcaa
    21241 aacgaccctg ttcaacatgg tttcgggcgt ttaccggccg gaccgcggac gggtgatgct
    21301 ggacggacaa gacgtgacgg agcttgagcc tgatcggctc tgccggctcg gcctgtcgcg
    21361 cacctatcag aatctgcaga tatttttccg cctgaccgcg gccgagaacg tcatggtcgg
    21421 ccgtcaccgg catgagagcg ccaacgtgct ggcgcacttg ctggcgctgc cctcggtcgc
    21481 gcgccagaac cgcgagacgc gccgcaatgc ggtcgccctg ctggcgcgca tcggcatgga
    21541 gcgctatgcc gatacgccag ccggttccat gccgtacggc gccttgaagc gtctcgaaat
    21601 tgcccgggct ctggccaccg agccacgttt cctgcttctg gatgagccgg ccgccggctg
    21661 caacccggtc gaaacggatg aggtcgaggc ggtgattcat gccatcgcca aggacggcgt
    21721 cggggtcgtc ttggtcgagc atgacatgaa gcttgtcatg cggatttcgg atcgcatcct
    21781 ggtgctggag cagggccgtc ctctggcgga agggcagccg cgggatattc gggagaatcc
    21841 ggctgtaatg aaagcctacc tcggtgagca tggcgcgcgg gaggcagctc gtgcttgaag
    21901 tcgcgaacct gacgtcatcc tatggccgca tcgaggtgtt gcacggcgtt tcgctgaccg
    21961 tcgcggcggg cgagattgtc accatcgttg gcgcaaacgg ggcaggaaag acgacgctcc
    22021 tgcgcgctat atccggtgtc cagccgatca ccgccggcac gatttccttc gaaggacgcc
    22081 tgatccagtc gattgccgga catgatcggg tcggcctcgg cattgcccag gtgccggaag
    22141 gacgccaggt gtttgccccg ctcagtgttg aggacaatct ccgcctcggg gcatggagtc
    22201 gccggggcgc ggatgttctg gacgacatcg agcgcgccta tgcgatgttt ccggtcctga
    22261 aggagaagcg ccaccagcat gccggcgccc tgtccggtgg ccagcagcag atgctggcta
    22321 tcggccgagc tctcatgtcc aagccgaagc tcatcgtgct ggacgaaccc tcgatgggat
    22381 tggcgcccat tctcgtcgat cagatcttcg gggtcattcg ggcgttcaag caggccgggc
    22441 aaaccgtgct actggtggag cagaatgcct ttgccgcgct ttcgatcgcc gaccgcggct
    22501 atgtcctgga gacgggtcgt atcgccctga gcggacgggg cgccgatctg gtgcgcgacg
    22561 aacgggtgcg tgctacctat ctgggtcagt agacacccag tccatggccg caccaacaca
    22621 agaggacttc catggcgctt tcctacatga ccggcttcgg caatggattc gagaccgaag
    22681 cgctgccggg cgcattgccg gtcgggcgca attcgccgca gcgctgcgcc tatggactct
    22741 acgccgagca gctgtccggg tcgcccttca ccgcgccccg cgcctccaac gagcgctcct
    22801 ggctctaccg catccggcca acggtcagac actggggacg gttcaggaag gcggacatcg
    22861 gtctctggcg gacggccccg gcgcgtgagg tcgaggcgcc gcccgcaccg taccggtggt
    22921 cgccgttgcc gctgccgagc gaatccgtca gctttctgca gggcgtgcgc acgataacga
    22981 ccgctggcga cgccggagcg accgcgggca tgtctgcctc gatctatctc gtgacccgtt
    23041 cgatggtcga tgagtgcttc tacaatgcgg atggcgaact gttgttcgtg ccggagcatg
    23101 gcgggctgcg tctcgtcacg gagttcggga tcattgaggt cacgccggga gaaatcgcgg
    23161 tcgtgccgcg cggcatcaag ttcagggttg aagtcccgga tgggcagggc gcggcgcgcg
    23221 ggtatctgtg cgagaactac ggcggcgcct ttaccctgcc ggagcgtggc ccgatcggcg
    23281 cgaactgtct tgccaaccag cgcgatttcc tgacgccggt cgcctgtttc gagcagcagg
    23341 acaagccgac cgccatgtat gtgaaatggg gcggggccct ttgggtaacg gacctcaagc
    23401 actcgccgat tgatgttgtg gcgtggcacg gcaactatgc cccgtacaag tacgacttgc
    23461 gccggttctc acccgtcgga ccgctgctgt ttgaccacgc cgatccctcg atcttcacgg
    23521 tgctgacctc gccctcggag acggcgggca cggcgaacat cgatttcgta tgcttcccgg
    23581 agcgctggat ggtggctgag aacaccttcc ggccgccctg gtaccatatg aactgcatga
    23641 gcgagttcat ggggttgatt tacgggcagt acgacgcaaa gccggagggg ttcgtcccgg
    23701 gcggtttctc gctgcacaat accatgctgc cgcacgggcc ggaccacgac agttttgaaa
    23761 tgaactcgac agtcgaattg aaaccgcaca agctcgcgaa caccatggct ttcatgttcg
    23821 agacgcggtt cccgcaggcg atcaccgcct atgcgctgaa cgcgcccgaa ctgcagcaca
    23881 gctatggcga ctatgggcac gccctgaagg atcattttga tccgcatcgg cgcgacaggt
    23941 aaggacggac atgaagctcg ccaccctcaa ggacggctcg cgcgacggac ggctggtcgt
    24001 cgtctcgcag gatctgacgc gacataccga cgctgcgggc atctgccgga cgttgcagca
    24061 ggcgctcgac ggctgggagc gctatgcgcc ggcgctcgcc gacctcgcgg agcaactgga
    24121 acacggcagt gtgcccgccc agcgcttcca cgagcatgac tgcgcctcgc ccctgccgcg
    24181 cgcctaccag tgggcggacg gctcagccta tgtcaatcac gtggagctgg tgcgcaaggc
    24241 ccgcggtgcc gagatgccgc cgtcgttctg gaccgatccg ctgatgtatc agggcggttc
    24301 cgataccttt ctcgggccgc gtgatcccgt gccggtggcg accgaggcct tcggaattga
    24361 cctggaaggc gaagttgcgg tgatcgtcgg tgacgtgccc atgggggcga caccggaaat
    24421 cgccgcaagg gccatccggc tggtgatgct ggtcaacgat gtttcgctgc gcaacctcat
    24481 cccgaacgaa ctggccaagg gcttcggctt tttccagtcg aaaccatcct ccgccttctc
    24541 gccggtggcg gtgacgccgg atgacctcgg gccggcctgg gtcggcggta aggtgcgctt
    24601 gccgctgctg accttcatca acggcaagcc gttcggaaag ccggacgcag gccaggacat
    24661 gacattcgat tttccgacgc tcatcgccca tgcggcgaag acacggccgc tttcggccgg
    24721 cacgatcatc ggttctggca cggtctccaa caaggatcgt gacggtggtc cgggccgtcc
    24781 tgttgccgat ggcgggctgg gatacgcctg cgtagccgaa atccgcatgg tggagaccat
    24841 tcgggacggc aagccgacga cgccgtttct ctccttcggt gacgtcgtcc gcatcgaaat
    24901 gaaggacgcc aggggcaagt cgatttttgg cgccatcgag caggaaatcg tcgcccatac
    24961 gggatcgcag gcatgagcgg ctttgcttcg acgaacgacc ttgccgacaa gaaggtcagc
    25021 ttttccgaga tcggtccggg tctctacgcc tacaccgccg agggcgatcc caatacgggc
    25081 gtgatcatcg gtgatgatgg cgtgatgatc gtcgatgcgc aggccacgcc cgcgatggcg
    25141 caggacgtga tcgcgaagat tcgctccgtc accgacaagc cagtccgcta cgtgctgctc
    25201 tcgcattacc atgcggtgcg tgtgctcggc gcctcggcct atacggatgc gcatgcgatc
    25261 ctggcttccg accgaactcg tgccctgatt gtcgagcgcg gcgcgcagga catggattcg
    25321 gaagtcggcc gttttccgcg cctgtttcgc ggcaaggaaa cgatccccgg cctgacctgg
    25381 ccaacgctca ccttcaccgg ggaaatctcg gtctggctcg gcaagcggga agtgcgcatc
    25441 gcgcagccgg ggcgtggcca cacggcgggc gacacgattg cctgggttcc cgatgcgggc
    25501 gtgatgtttt ccggcgatct cgtcgagtac aagtcggcct gctattgcgg tgacgcgcat
    25561 ttcagggatt ggccggaaac gctcaagaag ctgaaggcgt atgatcctgt ggcgttggtt
    25621 cccggtcggg gcgatgcgct ggttggcggg cagaaagtct ctgccgccat cgccggcacg
    25681 acggattttc tcgcagcgct ctatggcgcc gtcgaagcct ccgtgaagcg tggggaaagt
    25741 ctgaaggaag cgttcgcggc ggtgcgcaag gcgatggacg ggccgtttgg atcctacgcc
    25801 atctacgaac attgcctgcc attcaatgtc tcgcgcgcct atgatgaagc acggggcatc
    25861 gatcatccgg tgatctggac ggcagagcgc gacgtggcga tgtggaaggc gttgcaggcg
    25921 gggtgacgct gaagcggtct tgacgtctgg gtcaatccac acgctgccgt acgccttgaa
    25981 ctggcggtct cagcctcggc cgaacacctg agacgttgaa ccaccgttgc tcagcctgag
    26041 gctatggatt ttgatggtcg cgaccagggg gccgcacatg tcgatgacgc agttgaacgc
    26101 cgaagatgtt cgccgccgcc tgccatggga tcgactgatc ggcgccatcg aagcacagtt
    26161 ttccgaaggc gcgcaggtgc cgccgcgccc ggtgttcgat gtcgccaatc cgaaggggca
    26221 agccggcgcg ttgctgctca tgccggcgtg gacggagggg gagacgctcg gggtcaaggt
    26281 tgtcacattc tttcccggca attccgaact tgggctttcc accataagcg ccgcctacct
    26341 cctgttcgat gccgcgacgg ggcagatccg cgcggtctgc gacggggatg agatcaccgt
    26401 gcgccgtacg gctgccacgt cggctgccgc ggcgcggcgg ctggcccgga gcgatgccaa
    26461 gcgcctgctt gtggttggct ccgggcaact cgctcccaac atggcggaag cccacgccgc
    26521 agtgcgcgga tacgatgtga ttgagatctt cggacgcagt cgcgacaagg ctgccgcggt
    26581 cgtgtcgcgg ctcgtccgtg ccggactgcc ggcacgcgtt gccgatgacc tcgaagcatc
    26641 ggttcgcgcg gcggacgttg tatcgtgctg cacctcggca acggtccccc ccgtccaggg
    26701 aacatggctg aggccaggca ctcatgtcga tctcgtcggg ggcttccggg acgacatgcg
    26761 cgaggtggac gacaccgccg tcgcccgtgc gagccttttt gtcgacgaac gcccgggcgc
    26821 gctgaagtcg ggcgatctgg cgcagccgat ccgggccggg ctcctgcggg aaagcgacat
    26881 ccgggcagac ttccgcgaac tcgtcacggg aagccacgta ggacggacat cggctcagga
    26941 aatcaccctg ttcaagtccg ttgggcacgg gctggaggac cttgccgctg ggcgcatgat
    27001 agcgcggacg ttgtagggcg accgaagtcc gggtcgctgc tgccgcgctt ggatcgtcgt
    27061 tgacggtgtc ctcatgcccg cgcataagcg cgcaacgccg tttcgaggat ttccgccatg
    27121 tcgactgccc tgcttcccac cgacgcccgc gccattgccc ggcagacggc gaagatgctg
    27181 cttgagattg aggcggtgct gtttcgggct gaccagccgt ttattttcac atcaggttgg
    27241 gcaagtccgg tctacaccga ttgccgcaag atcatttcct atccgcgtct gcgtgcggcc
    27301 ctcatggatt tttgcgccgc aacggtcatg cgcgaagtcg gctacgagca gatcgaggtg
    27361 atcgctggtg gcgagacggc tggaattccc ttcgccgcct ggatttccga gcgactcggc
    27421 ctgccgatgc agtacatccg caagaagccg aaggggtttg gccgaaacgc ccagatcgag
    27481 ggcaacgtcg tcgagggtca gcgcgccctt ctggtcgagg acctggccac cgacgggcgc
    27541 agcaaggtga atttcgtcaa cgcccttcgc accgccggga tgacctgcaa ccacggcatc
    27601 gtgattttct attacgacat ctttccgtcc ggaaagcagg tcatgaagga cctcgatttc
    27661 cagttgctgc atcttgccac ctggtgggat gttctggacg tggcgaagga aggccggtac
    27721 ttcgacgctg gcgtgctgcg cgaggtcgaa agctttctcc acaagccggc ggaatggtct
    27781 gccgcccacg gcggcatttc ggaattcggc aaggccgggg agtagggcac ccgccattcg
    27841 acaggcccgg gacaggggcg taaggtgccc gggattggaa gaaggcccga aacacccact
    27901 ggtgccagca tgtctcttgc ccgccgcatc cgcctgccgc gtaccgacct cgacgtttcg
    27961 ccgatctgcc tcggcggcaa ccggcttggc ggcgaactgg atcaggccgc ctccttcgcg
    28021 ctgctcgatg ccttcgtggc ggcgggcggc aatttcatcg ataccgccca tgtctatgcc
    28081 aactggctga aggatgccga gcgttcgtgc tcggagaaga cacttggccg ttggctgagg
    28141 gcacggggcg gcacgagggg cgtcgtgatc gccacgaagg gcgggtcgcc cgaggcggag
    28201 gcgccggagg tcaggcggtt ggacgagggg tctctgcgcc gcgatctggc cgaaagcctc
    28261 gagttcatgg gggtctccac catcgacctc tactacctgc atcgggacga tccggcccga
    28321 ccggtcgagg agctgctggc actgttggag gacatgaaga cgaaagggca gatccgtcac
    28381 tacggcgcgt ccaactggtc ggtggcgcgg ctggaagagg cggagagcgt ttgtcgcgcc
    28441 cgaggctggt caggctttgc cgcgaaccag tgcgaatgga gctttgctaa gcgcaatgcc
    28501 ggcaccactg gtgcagacat ggtgcagggc gatgcagcga tggctgcctg gcaccgcagg
    28561 cacggcattg cggccgttcc ctattcgtca caggccaagg gctggttcga caaggtcgcg
    28621 accggccggc aggagccgct gaccgaccgc ctctacgaca acgccgggaa ccgcgcgctt
    28681 gcgccggtcc tggcggctgc cgcgcaggct gtcggtagta cgccgactca ggcgatgctg
    28741 gcgcgcatga tgcaggcgcc gttccccttt gttccgatca tcggaccgcg ctcggtggag
    28801 cagatgcgtt cttcggtcgc aagccttgcc ctgccgccgg tcgagcacgg cctgccggcg
    28861 gtgtgaaaac tcagcccccg gccactgcgc ggcctatgac caggcgctgg atatcggagg
    28921 cgccctcgta gatctggcac accctgacgt cgcgatagat gcgctccacg gcgaaatccg
    28981 cagtgtagcc gacgcctccg tgaatctgga tggcgtccga gcagatcgat tcagccgcct
    29041 ccgaggcgaa cagcttggcc atcgaagcct ccttcaggca ggggcggccg gcgtccttga
    29101 ggctggcggc atgccagacc agttgccttg ccgcttcgag ccgcgtggcc atgtcggcga
    29161 gccggaagga cacggcctga tgctgcacga tcggcacgcc gaaggccttg cggtcgttcg
    29221 cgtatctgac ggcctcttca agcgcggaac gggccatgcc gacgcattgc gccgcgatcc
    29281 cgatgcgtcc ggattcaagg ttggccagcg cgatcttgta gccctcgcct tcggagccga
    29341 tcaggttgtc cgccggaaca cggcagtttt ccaggatgat ctgggcggtg tcggaggcgt
    29401 gctgtcccat tttgtcctcg acacgcgaca cgatgtagcc gggcgcatcg gtcggcacga
    29461 ggaagcagga aatgccgcgc ttgccggctg cggcatcggt caccgcgaag acgagcgccg
    29521 cctgaccatg cttgcccgaa gtgatgaact gcttcgtgcc attgaggatg taggcgtcgc
    29581 cctcgcgccg tgccgtcgtc ctgagcgccg aagcgtccga accggtatgt ggctcggtca
    29641 ggcagaagca gccgatccag gcgccgctcg cgaatttcgt cagatagcgc cgcttctgtt
    29701 cgtcggttcc gtaggcgagc ggaatgccgc aggcgagcga gttctgcacc gacacgatgg
    29761 tggatgtcgc cccgtgtccg gccgcgatct cctccagcgc cagaaccagc gagacatagt
    29821 cgaggccggc gccgccccac tttgccggca cggtcatccc gagagcgccg agagcgccca
    29881 gcgcctgcag cgcttcgcgc ggaaaggtct tctcacgctc ccaccgcgct gcgttcggcc
    29941 agagttgctc ctgcgcgaag gcgcggatcg tgtcgcggat catttcctgt tcagccgtca
    30001 ggatcatggc tggacaacct cccggttctg ttcacgagca gcctatcagg tgcgaagccg
    30061 gctgccacta acgccggatc ggcaaaaagg cgcagcggtg cgttcaggcg cggtagcccg
    30121 aaagcacggt ctccgcgatg gtggcagcca gcgtccgacg ctcggccgcg ccttccccgg
    30181 gccgaggctg gtaccagacc gcgacccagt tcagcgcccc aagcaacgtg cgcgaggcaa
    30241 gcgctgcctc cgggaaccgc ctcagggagc catcgcgagc accgtcttcc aggacgcggc
    30301 ggaacagcgc ctcgtagatg tcacgcagca tgatgaggtc gctcaacgtc gcctgatgtt
    30361 cggcggcgcg gccgaagcgc cagaggtccg gggcgagcgc cgccgttcgc tggaaaggca
    30421 gggattccat cagcgtcagg gcatgcgtct gcgccatgtc ccgcaaccgg tcaccggcca
    30481 gcccgggccg cgagacggcc ggtgcgaccg cctgcgtcag catgtccatg gcccggcggt
    30541 agaccgcgaa gaacagttcc ggcttcgaat tgaaatggtg gtagaccctg cccttggtgg
    30601 cgcccagccg ctccgccaca tcatccatgg acgtgttctc gtagcccttc tgcatgaagc
    30661 actccgccgc ggcggcgagc acttcctcgc ggcggcgatc agccggagtc tcggcgggga
    30721 gaatcgctga ttcagccggc atcgggcagt ttgtggccgg cgaactgctt gcgcagcgtc
    30781 agtttggaca ccttgcctgt cgctgtcagg ggcaacgaat cgacgaatac gacgtcgtcg
    30841 ggcaactgcc atttggcgat cgccttttcc agagttgcaa gcacatcctc gcgcgtcggg
    30901 ttggctcccg gagccttgac cgcaaccagc agcggccgct cctgccactt ggggtgaggc
    30961 acgccaatca ctgcgcaggc ggcaacgccc ggcgtcgcca tggctgcgtt ttccacatcg
    31021 atcgaggaaa tccactcacc tccggatttg accaggtcct ttgtgcggtc gacgatcacc
    31081 aggtagccgt caggcgtgat cttggcgaca tcaccggtgc cgaaccagcc gtccttgtcg
    31141 aagaccttgg cgctggcctc ggcattctcg tagtagccgg agatcaccgt cgcggcgcgc
    31201 acgaacaatt ccccggtcgc ttcgccgtca tgcggctggc gctggccgtt ctcatcgacg
    31261 atcttcagtt cgacgccaaa cacccgccgt ccctgccgtg ccttgatcgc catgcgctcc
    31321 gcaaaggact ttgcctggtc cttgacggcg atgaccccat gggtgccgat cggactcatt
    31381 tcggtcatgc cccaggcatg aacgatggac acgtccatct tctcgaacgt ctcgatcatc
    31441 gaagccggcg ccgccgagcc gccgacgatg acttctccga atccgttcgg cttgcggccg
    31501 cgcttctgga tttcggcgag caggccgagc cagaccgttg gcacgcccca ggcggaaaac
    31561 accttttctg cctccatcat gtcgaacagg ctggcgccat caagccgagc gccgggcatg
    31621 atcagattga cgccgatcat cggcgcgctg aacggcaggc cccaggcgtt gacgtgaaac
    31681 agcggtacga caggcaggat ggtctgcccc ggccttaggc ctgcgatgcc ggtggtcatg
    31741 ccgaacaggg cgtgcaggac cgtcgagcga tgggaataca acgcaccctt gggctcgccg
    31801 gtcgtgccgg aggtgtagca taggcagcag gccgtgtttt cgtcgaggct cggccaggcc
    31861 atggagcgcg gttggccggc aagcagatcc tcgtaggcga gaacgccctc gaggccttcg
    31921 ccggtcggca ggtggcccct gtcgcagagc gcgacaagtt tcaggtcctt cggccaatgc
    31981 gcgcgcatct tggcgaccag cggcgcgaga ttgatgtcaa agaacagcgc tttgtctcgg
    32041 gcgtgattga cgatgtaggt cagttgctcg ggaaacaggc gcgggttgat ggtatggcac
    32101 accgcgccga gacccatcac cgcatagtaa agctcgaaat gccgatagcc attccaggcc
    32161 agcgtggcga tgcggtctcc cggcttcacg ccaagtgcct tcaggccgtg cgccagttgc
    32221 gctgaccgct gcgccgcctg cgcataggtc tggcgatgga acccgccttc cagcgcggcg
    32281 gaaacgatct cgctttcggc gtgcatttcg gccgcatagt caagaatggt cgagacgttc
    32341 agcggaacgt ccatcatcat gccgcgcagc attctatcct cccgcatctc cgagctttct
    32401 cggctgccag agccagtcgg tcaatgggct tgccataaaa catacttgtg agtatgtccg
    32461 tgtctggacg gggcgtcaac ggcggtccgt cagcggagga actggagcga tgaccgacct
    32521 tgccgccttt cgggccgaga cgcgtgcctt ccttgaagcg aactgtccgg tgtcgatgcg
    32581 ttcacctgtc cgatcggatg aggacatctg ctggggcggg cgcaattggc gcttccagtc
    32641 ggacgatcag cgcctgtgga tggagcgcat ggcggcgcgc ggctggaccg tgccgacctg
    32701 gccaaaggcc tatggcggtg gcggcctgag ccacgaggaa gccaaggtcc tgaaggagga
    32761 gatgtccgcc attggcgcgc ggcagccgct gctctccttc gggatctgga tgctgggccc
    32821 ggcgctgctc gctttcggca acgaagcgca gaagctggaa catctgccga ggatcgcccg
    32881 cggcgaaatc cgctggtgcc agggctattc cgagcccggc gccggctcgg atcttgcctc
    32941 gctccagacc agggccgagg acaagggcga ccactacgaa gtgacggggc agaaaatctg
    33001 gacgagttac gccgacaagg cggactggat cttctgcctg gtccggacca atgccaccgc
    33061 gcctaaacac cttggcattt cgttcctgtt gttcgacatg acctcgcccg gcgtcaccac
    33121 ccggccgatc acgctgattt ccggcaagtc gccgttctgc gaaaccttct ttgacgcggt
    33181 gaaagtgccg aaggcgaacc tcgtcggcac gctggatcgt ggctgggaca tcgcgaaata
    33241 cctgctcacc cacgaacgcg agatgatcgg cgagggcggc tccggcctgc tcggtggccg
    33301 ctcgccgggc cagatggcga cggacgccat cggcctggac gccacgggtc gactggccga
    33361 tcccttcctg cgggctgatg ccgcgatggc ggaagtcgac gccctggcct ttgccctcac
    33421 catggaccgc tacaaggatg aagcggcgtc tggggggcgt ggcgtcggcg acaggtcggc
    33481 gatcctgaag tacgcgggaa cagagctcaa caagcgtaac cacgaactga tgatgcgtgc
    33541 cggcggccat gaggcgctgg aatgggaagg gcagcggtcg aatgacggca gcctgcctcg
    33601 cgcctggctg cgtacgaagg ccaattccat cgagggcgga acatcggagg tgatgctcga
    33661 catcatcgcc aagcgcctgc tgcaactgcc gtccgcctga ggtccgcaat gctcatcctg
    33721 aacgaagacc agaagatgct gcaggaaagc gcgcgcggct tcctggaaag ccgttcgccc
    33781 gtgtccgccc tgcgcaaact gcgcgacgat cgctccatcg acgggttcga ccgtgcactc
    33841 tgggccgaaa tggcggagat gggttgggcc ggtattctgg tgccggaaga acacggcggc
    33901 cttgggtttg gctatgtcgg cgccggcatt ctttgcgagg aaatgggccg cacgctggcg
    33961 gcatcgccct tcctctcgac gagcgtcatg gcagcgacgg ccctgcgggc atcggggacc
    34021 cttgggcacc agcagcgttg gctgccgggc attgctggcg gcagggctat cattgccgcg
    34081 gccgtggacg aaggccgcgt tcatgatccg gccggcacgg cgctcgccgc cgaaagacac
    34141 ggcaacgggt tcctcctgcg cggatcgaag gccgtcgttg tggatgggca tgtcgccgac
    34201 gccttcatcg tcgccgctcg caccgcaggc gcgccgggcg agaaggacgg agtcaccctc
    34261 tttctggtcg atgccaaggc gaaggggctg agcatcgagc gcacgatcat gctcgacagc
    34321 cgcaatgccg cgtccctcca gtttgacggc gtcgaggcca cgggcgccga tattcttggc
    34381 gagacggacg caggctaccc cctgctagaa gtcatgctcg gggcgggtcg tgccgggctc
    34441 gcggcagaac tcgcgggcat cgctgccgag agcttcggtc gcaccgtatc ctatctgaag
    34501 gagcgcaagc agttcggccg caccatcggg tcgttccagg cgctccagca tcgtgccgcg
    34561 cacctgtttt cggagatcga gatcgtcggt tccgctgtgc tcaacgcact gcaggcgctg
    34621 gaccgcgatg cgaacacggc ttcgcttgcc gtctcggtcg ccaaggccaa ggcggcctcg
    34681 gttgctcggc ttgccgcaca ggaatcggtg cagatgcacg gaggcatcgg catgacggac
    34741 gcggtggata tcggcctctt catgaagcgg atgcagtcag ccagcatgct ctttggcgat
    34801 gtgtcgttcc atgccgaccg ggttgccttg gcgcggggat tttaagggtt gcctttctcg
    34861 tcgcgggaac gcgatggatc ggcgagggcc ggattgaaga caagagagag gggctatgac
    34921 caaacaggtc acattcaatg acatgcgcgc cagcgttggc acggaagtcg gcgtctccga
    34981 ctggatcgag atcacccagg acatgatcaa ccgcttcgcg gacgtgacct tcgatcacca
    35041 gttcatccat gtcgatgtcg agaaggcgaa gctctcgccg ttcaagacga cgatcgccca
    35101 tgggttcctc accgtgtcgc tgctctccgc catggcctat tccggccttc cggacatcaa
    35161 ggggcgggtc atgggcgtca actacggctt caacaagttg cgtctcgtct cgccggttcc
    35221 gtcgggagcg cgggtgcgtg cgcggttcac gctgaaggcg cacgacgaaa aactgccgaa
    35281 gcagcacacc tttaccttcg acgtcacggt cgaggtcgaa ggatcgggca agccggcgct
    35341 ggtcgcggag tgggtcactc tgtcgttcga aggatgagga acggaactat gagcatccgg
    35401 tttgatgggc gtgtggccat cgtcacgggc gcaggcaatg gtctgggccg ctgccatgcg
    35461 ctgggccttg cagagcgcgg cgcgaaggtg gtcgtggtcg attttggcgg cgcgcgggat
    35521 gggtccggcg gttcctcgga ggcggcgcgg aaggtcgtgg ccgaaatcga ggcgaagggt
    35581 ggcgaggcca tggcgcatgg cgccaacgtc accgacatgg cgcaggtcac cgacatggtg
    35641 gaaaaaacca tggcgcgctg gggccgtgtc gacatccttg tcaacaacgc cggcatcctg
    35701 cgcgacaaga ccttctcaaa gatggatctt gccgacttca agacggtcgt cgatgtgcat
    35761 ctgatcggct cggttaactg caccaaggcg gtttgggaga tcatgcgcgc gcagaattat
    35821 gggcgcattg tcttcacctc gtcaggctcg ggcatctatg gcaactttgg ccagtcgaac
    35881 tacggcgcgg ccaaggccgg cgtcgtcggg ctgatgaacg tgctgcacca ggagggctcg
    35941 aagaacgaca tccgcgtcaa cacgctcgcc ccgaccgcgg cgacccggat gaccgaggaa
    36001 ctgctgccga aggaagcgct cgacctgatg acgccggaaa gcatcacgcc cggcctgctg
    36061 gtcctcgtcg ccgagaacgc cccgtcgaag ttcattctct cggccggctg cggaaccttc
    36121 gcacgcattg tcatcgagga gaccgatggc gtctatttcc cggaaacgga acggacgccg
    36181 gaacagatcg cggcccggct cggggaaatc tgcgacacca ggggcaaggc ccccttgccg
    36241 ggagccttcg accagacgaa aaagctcata gccaaggcgg cggctgcaaa gggcgtatcc
    36301 gtgcccggcc tgtcgtgacc ctgttcaacc ggaaaggatt ttccatgcgt gaagccgtcg
    36361 tcgtctccac cgcccgcacc ggccttgcca agtcctggcg cggtgctctc aacaacacgc
    36421 acggcgcttc gatgggcggc cacgtggtca agcatgcgat gcagcgcgcc aaggtcgatc
    36481 ccggcgaggt cgaagacgtg attgtcggct gcgccaatcc cgaaggcgca accggctgga
    36541 acattgcccg ccaggtggct ctcgccgccg gctgcccggt ttccgtgtct ggtgtgacgg
    36601 tcaaccgctt ctgctcctcg ggcctacaga ccattgccct ggccggacag cgcatcatgg
    36661 ccggggaggg cgacgtctac gcagcgggcg gacttgaatc gatttccctg gtgcagaacc
    36721 agatgaacaa gcacaagttc atggatgcgg gcctgaccaa gtccaagccg gaagtctatt
    36781 ggccgatgct gcagaccgcc gagactgtgg ccagacgcta caacatctcg cgcgaggtgc
    36841 aggaccacta cggcgttgag agccagcgcc gggccgccgc cgcccagcag ggccgcaagt
    36901 tcgatgacga gatcgtgccc ttcaccacca tgatgaccat ggcggaaacg aacgagaaga
    36961 aggaggtcgt gcgcacctgg gagcaggagg tgacgctgaa gaccgacgag ggcatccggg
    37021 cggacaccac ctacgagggc gtttcgaaga ttcgcccggc cgtcgagggt ggcgtgattt
    37081 ccgccggcaa tgccagccag ttctcggatg gcgccagcat gtccattgtc atggattcca
    37141 agctggccga gaagcgcggc ctggcgccgc tcggcatctt caggggcttt gccgtcgccg
    37201 gctgcgaacc cgatgagatg ggcatcggcc cggtctatgc cgtccccaag ctcctgaagc
    37261 gccacggact cacggtcgag gacatcgacc tgtgggagtt gaacgaggcg ttcgcggtgc
    37321 aggtcatcta cagccgcgac aagcttggca ttccgaacga tcggctgaat gtcaacggcg
    37381 gtgccatcgc cgtcggccat ccctatggca tgtcgggtgc gcgtctggtc ggccacgcgc
    37441 tgatcgaagg ccggcgccgc aaggccaagt atgtcgtcgt caccatgtgc atcggtggcg
    37501 gcatgggcgc cgccggactg ttcgaggtgg cgtgacaggt acccggtgcg ccgctcccat
    37561 cctgggagtg gcgcgcctgc gtctatggct cgatggtgaa gccccgggtg acgaccggct
    37621 tgccgtcaac gaagaggtcc acgcggtagc gcccgatagg ccaatcgcgg ggcagtgaga
    37681 catccatctg caactcggtg tcttccaccg ggcggtttgc gtcatcctgg cgatgcagct
    37741 cccgggaccc cgaggtggtt tcgatggcgc tgaataccca ggtcaggttg atgccgttcg
    37801 tgttcgcgcc ggaaaccttc accgaaaaat tcaggtgccg ataggaggac ctgaccttct
    37861 cgacaggttc cacctggtcc ggaccggtca ctttctcgat ggccacgtca ccgaaactca
    37921 gttcggtctc gcgcggcggg tcggcgatca cgcgatacgg caaaacgccc acgggctggt
    37981 cgttctggaa aaccaggact tcatagtgac cgaccggcca ttttcgcggc agcttcacct
    38041 cgatcggaac cagtccctgc tcgttcaccg tggcactggc ccggaagacg acggccggcg
    38101 ccgaggctgt cgtggaccgg gcgcgcacgg cgaagttcag ggtctgaccc acagccgatt
    38161 tgtcgaaagt gatcagcatc agcttgcgct gctggtgctc gttgaactgg gttgagggaa
    38221 gcggcgtccc aaaatgcccc ttgggcgtat ccggaagcaa ggcaagcccg gtgggccgga
    38281 tcgattcctg ggcggtttgc gcagccaccg gcgtgagcat ggcaacaaat acggcggcca
    38341 tccaggttcg acgcatgcgt cccctccggt tccggaacat ccaaatcact ggctggaacc
    38401 tgccgtcgcc ttcacgtctt ggcaagcttg cggcactgac cccgttgact gataactgag
    38461 aaatgataaa aacgaaaaat catcagtgtt cacgttgggg atcatggcac atcgcccaag
    38521 cctgtctgcc gaagaaacgc gtggccgcat tctcgaggtg gcggaggagc atttccgccg
    38581 cgtgggatat gccaagaccg cgatcgccga catagccgcc gttctcggca tgagcccggc
    38641 caatgtctat cgcttctttg cttcgaaggc ggacatcaag gcggccatcg cagacaaact
    38701 catgagcgcc ggacgcgcca tactgcgcca cgaagccgag ggcgcgggta cggctgctga
    38761 gcggctgctg cgggtggcca tgctcctgca tcgctacaac aagcaacagt ttaccgccga
    38821 tcgtcgcctg cacgacatgg tcgaggcggc gatgagcgag aactggaccg tcatccagga
    38881 ccatctggag cagatcacgg gcatcttcgc cgacattatc cgctccggaa tcgcgtctgg
    38941 cgagtttgcg cttcgggacc cactggaggc cgggcgcacg ctccagcaga tgatggcctc
    39001 cgtttgccat ccgacgctga tcgcccagtg cgtcgacgtt gatctggaag agcaaaccga
    39061 acgtatcgtt ggctatttcg tgcggtcact gaaggttacg gggtgaacca tgcgtcttga
    39121 ggctaaggca atcctggccc ttcttttgcc ggtactggct gcctgcgggg aaggaacggc
    39181 ccgccagaag cagccgtccg cccagccccg tgccgtggtg gttgaggtgg tgcggtttgc
    39241 gcctcgcgcg cccgaacgca gtctggttgg cgtcattcgc ccgcgcgtcg aaaccgacat
    39301 gggcttccgg gttgccggaa aggtggcgga acggctggtg cagcagggcg agcgggtgaa
    39361 ggcgggtcaa cccttgttcc gcctcgatcc ggctgatctc aatctccagc gcgagcaggc
    39421 ggcggcggag cttgccgcgg cgcgcgccag ccttgccagc agcgccgcgc aggatcagcg
    39481 ctcgggcgaa ttgaagaaat ccggctgggt ttcacaggcc aacctcgatc agcagcttgc
    39541 ccgcacggcg gatgcacgcg ggcgcatgga gcgcgcagag cgtgcgatgg cgcttgccga
    39601 gaaccaggcc gcatatgctg aaatccgctc ggacgtcgat ggcatcgtca ccgccaccat
    39661 ggccgaacca ggacaggtgg tcgcggccgg cgctccggtc gtgcgcgttg cccgttccag
    39721 cggctttgat gccgtcgttg cggtgcccga actgctcgtg gacagcgtgc gcggcggcag
    39781 ggccggcgtg acgctttggt cgcggcccgg cacgacctat cccgcgaccc tgcgggagtt
    39841 tgcggcctcg gccgacgcgg tgacccgcac gtttcaggcc cgcttcgctg tttccgcgcc
    39901 tgaagagacg cttgccatcg gcatgacggc gaccgtgaca gtggtggagc cggatgccgg
    39961 caagatcgcc agactgccgg tctcagccct gtattccaat ggccggggcg cctctttgtt
    40021 cgtcgttgat ccggcggatg gtgggctcga tctcagacgc gtgacggttg ccggctacga
    40081 aggcgcccat gtcctggtga ccgaagggat aaacgacggc gaacaagtcg ttgcctttgg
    40141 cgttcagaag ctcgatccga gccagaaggt tcgcgtcatt gagaagcgag gctgagcggc
    40201 catgagcgac gtcagcgcct tcgatgcgcg caaatcccgg ggcaactggt cctactgggc
    40261 ggtcacgcat ccgacgctca ccctgttcat gatcatggtg atcatggcgt ccggaatggt
    40321 ctccttcatg cggcttggcc gttcggagga tccatccttc accatcaagg tggccgtcat
    40381 caccgtgagc tggcccggcg cgaccgcgca ggaagtccag cagcaggtcg ccgaacgcat
    40441 cgagaagaag ctgcaggaac tgccctggtt cgacaagatg cagacctaca ccaaggcgaa
    40501 tttcgccgcg atgcaggtcc agttcaagga cagcacgccg ccccgcgagg tgccgggcct
    40561 gttctatcag atccgcaaga agctggacga ccttaagccg gacctgcccg ctggcgtgat
    40621 cggcccgaac gtcaacgacg aatatggcga tgtcgacagc gtgctctaca cgctgacggg
    40681 cgatggcgcg ggttatgccg acatcaagcg cgtggcggag cggctcaaga gccggctcct
    40741 gcgcgtgccc aacgtcacca aggtcaacat ctacggcgcg caggacgaaa agatcttcgt
    40801 cgagttcagc cacgcgaagc tcggcacgct gggcgtgccg gtacaggcga tcttcgaaac
    40861 tctgcagaag cagaacgtgc tgacgccggc cggaacgatc gacaccggag tcgcgcgcat
    40921 tccgctgcgc gtttcgggcg cactggatgg cgttgatgcg gtcgcggcag ctccggtgga
    40981 agcgaacggg cggattttcc gactcggtga catcgcgacg gtcacgcgcg gcaccgtcga
    41041 cccgccgagc tatctcatcc gccaggaggc cgttcctgcg ctggcggtcg gcgtggtcat
    41101 gcagaaaggc gcgaacatca ctgagttcgg cgaagccctt gccgaggcca aggagcagtt
    41161 tctcgcggag gttccggccg ggatcgacat ccaccagatc gcggatcagc ccaaggtcgt
    41221 gaacctcgcc atcgccgaat tcctgcgggc tttcgcggaa gcgctggcca tcgtccttgc
    41281 cgtgtccttc ctgacgctcg gcctgcgcac aggcgtcatc gtggccctgt ccgtgccgct
    41341 ggttctcgcg gtggtgtttt ccgcgatgta tgccatgggc atcgacctgc atcgcatctc
    41401 gcttggcgcg ctgatcattt cactcggatt gctggtcgat gacgccatca tcgccgtcga
    41461 gatgatggtg gtgaagatgg agcaggggtt cgaccggttg tccgctgcgg cgcatgcctg
    41521 gacctcgacg gcctttccca tgctcaccgg cacgctggtg acgatcatcg gcttcctgcc
    41581 ggtgggcctg gcgaactcat ccgtcggtga atacgctggc ggcattttct gggtcaccgg
    41641 cctcgcactg gtcgtgtcgt ggttcgtggc cgtgctgttc acgccctatc tcggcatgaa
    41701 gctgctgccg gatttccggg cgctggaact ccggaagcac gatcgcgccg tgcgccgggc
    41761 cgccagacgc ggccggcctg c
//
LOCUS       DAOMXN010000009        35797 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_810_length_35797_cov_11.438560, whole genome shotgun sequence.
ACCESSION   DAOMXN010000009 DAOMXN010000000
VERSION     DAOMXN010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 35797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35797
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_810_length_35797_cov_11.438560"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            180..1595
                     /locus_tag="PK812_01975"
     CDS             180..1595
                     /locus_tag="PK812_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009737185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRJ68348.1"
                     /translation="MANPNDPSTQRPAPGAVSAAIAELTRTFGNRCVTNQSVRDNHAH
                     TRTYLPPQPPDAVVFPHDEGEVAAAVSIAARHGLPIIPFGIGSSLEGQVNAPLGGLCI
                     DTGQMKAIVAVHPEDLDVVVQPGVTRSELNAHLRDLGLFFPVDPGADATLGGMVATRA
                     SGTNAVRYGTMKDLVLALRVVLANGEIVTTARRAKKTSAGYDLTRLFVGSEGTLGVIT
                     EITLRLFGIPEAISAATCSFPSVKAAADAASLAIQSGIPLARVELMDAMAVKAVNGYS
                     KLSLPEQPLLLLEFHGSPAGVKEQAESFQAIAEECGGAGFAWTARPEERSKLWQARHD
                     AYWAGLTLKPGASCLSTDVCVPISRLAECIDQTAADLEALRIPGTIVGHVGDGNFHVL
                     PLFDPNIPEELGRVKQFCDRLVLRALAMEGTCTGEHGVGQGKMKYLDTELGAPALGAM
                     RAIKQALDPRNLMNPGKVVTV"
     gene            1653..5450
                     /locus_tag="PK812_01980"
     CDS             1653..5450
                     /locus_tag="PK812_01980"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HRJ68349.1"
                     /translation="MREFLTFIGIVLCLALAALMAGPHFVDWDHYRPQIENRLAASLG
                     MPVSVNGPVSVTLLPTPSLTLEKVRFGSADGGKASTFEADRLAVVVSATSLLRGEIHV
                     TEALIDAPVLKVRQGAPLLSMSGSGGVVRPEQISIESLRLRDGKIVVERDGQPDLRIT
                     GLSADLEANSLIGPAKGSGSFVVGTTPRTFRFALGKLEEGRSRLKALVEDAQLALRLD
                     ADGVLGLVGAGAGFEGSLHLSGNPTLGSDGKGPQLPVRVSTRSRIDGTRAVFDDVSMV
                     IGAEPQPLTLTGNAGMTFDGRRRVEATLSARSFDFDRPGPDGKPRAAVPVDLIRQAAA
                     LVPGGTGVEAPDFDLDVSVGGIIVGGQTIVGGRVAISKDASGIGIEALEGELPGQTRI
                     KLARSAKSPAGLLAGRIEAESRDPERFLRWLTGEPGVATSGLSLKASAELETSVDGFR
                     LREIGIERGDTRLSGEGSFLLAVPGQRPVPRVVLKLASPRLNIVDVPGLTFGEKSGES
                     PDIDLDFEVEATKLVFEGNETGRLMARIRRDGPIVSIERLAVTEFGGASLIASGTIGG
                     GARRVTVKLDANRVEALTALGEKLVPGAFMASVRHRSPLLAPALVVATFANDDADDSY
                     DIRADGHLAGTKVQAAGKLSGRTDLRLDLELGLESADGALLAAQIGGHRSPAVGAGPA
                     KIDLSMRGDPRGSLSTVLTGSMPGVDARVIGNIRIFQPFAPFAGDLTIRAADLGPVAA
                     ALGVTHPAIPKGTTATLSGHVESNLERITIENLKAQIGGQEYSGEIAFSLAENGKVAG
                     QIKTPYVDFRPVIGLALGAPRAGAAPWASTAFADPMAPPLPGDLWIEADRARLDDKTL
                     LNDAKFVLRFDRGAASVEYAQFALGPARVEGDVFLRRTAGTAFLSSKLKASGIRLEQM
                     GDGSAKGLLDVQAQFSGSGESPDKLIAALAGGGALTMRQVQAPRYDPDALGRLLAMPL
                     DKLGPLDAANLTRRLEAELERSHWTVAETRVPIVVVNGVVRTGATAIDSKDARHEAQA
                     ALDLGGMVLDVKVTTSSAAVPADWRGAHPQFSTQWRGPVPNLRRSLAVDSLVNGYLSL
                     ALVRDLQKTEVFEQDVRERSAHNRRLKADEAMRRRADELSRFEQIERERREKAAEEQR
                     QLEARLAEDARRRAEEEERARAEKAAEAERQRAAEEESQRQARLAEEEAARIQRIQRL
                     LQNPETMATPTPRRSVAPQPQQAPLAPPLSLNPPTPGRPRVAAPVEIRPLNAPSAAPD
                     TAR"
     gene            complement(5404..5667)
                     /locus_tag="PK812_01985"
     CDS             complement(5404..5667)
                     /locus_tag="PK812_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HRJ68350.1"
                     /translation="MSGRVIKRSVSIAGHRTSISLEESFWNDLKVLAARRSVSAATLI
                     AEVDGERRDTNLSSALRVHVLDAAKAGLLSAPYPAPQKERSTA"
     gene            complement(5664..5885)
                     /locus_tag="PK812_01990"
     CDS             complement(5664..5885)
                     /locus_tag="PK812_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4169 family protein"
                     /protein_id="HRJ68351.1"
                     /translation="MLAAAIMGEKMSAEIINLRRARKQRLRTEQEARANENRIRFGLP
                     KADRERLKQERDRQATRLEGHKRELDPDL"
     gene            complement(5917..8160)
                     /locus_tag="PK812_01995"
     CDS             complement(5917..8160)
                     /locus_tag="PK812_01995"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRJ68352.1"
                     /translation="MRADRTFDISRFLDLISDSYKTNADTRDRSVAYALKRLKSDNRT
                     LEDLVNRRTGELKSARFLFGAVLQNTAQGIIIFDDEDRIVACNQRAADLLELPEHLSR
                     EGTPIAAVIKHQWSIGEFDGNDKEATGWVGKGDVRAAPSVYERQRPNGTIIEIRTLLL
                     DGGGALRTFSDITERRRQENAAREAQELHRSLFENAAVAIYRATLDGQMTAANPEFAR
                     IHGYGSVEELLAAQNDRREWRYVKAGRREKFRRRLFRLGRVTDFVSELEHVDTGRRIW
                     SQETAWLVRDAAGNAVCYEGCIADITERKLNEQKIVHMAMHDALTGLANRRNLMDWLA
                     RRAETLPDQPLAVLCIDLDRFKEINDSYGHTAGDLLLWAAADRMSQSAGEEALVARLG
                     GDEFAVILPSAIHAEAAEEKAEALVAALCEDFDIGGRSVAIGASIGICFGAIDPKQTD
                     MLLKNADLALYQVKKNGGGRVRLFDASMDAEAQQRRRIERDLRSAVTGGEFKLVYQPI
                     LSLRSGEVSGFEALIRWQHPTHGMIAPSDFIPVAEESELINRIGNWVLNQACADFAAV
                     SAPLSVSVNVSAVQLRQSNFVTSILKCLSDTGLAPQRLTLEITESVLISDNAITRAAL
                     KALRGFGIRIALDDFGAGHSSLGYLRQYEFDRIKIDRSFISGGGADKADGAVVNAVIG
                     LGRGLGIEVIAEGIETEEQLDRLRFLGCDSVQGYFIGKPSALSDWPQLKPQSATLSLA
                     NLMRKSA"
     gene            complement(8402..9796)
                     /gene="fumC"
                     /locus_tag="PK812_02000"
     CDS             complement(8402..9796)
                     /gene="fumC"
                     /locus_tag="PK812_02000"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018427176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HRJ68353.1"
                     /translation="MTEKRTRTETDTFGPIQVDSTRYWGAQAERSLGNFKIGWEKQPA
                     SIVRALGIVKRAAAQANMKLGKLDTKLGAVIVQASQEVIDGKLNDHFPLVVWQTGSGT
                     QSNMNANEVISNRAIEIMGGEMGSKKPVHPNDHVNMSQSSNDTFPTAMHIACAEEVVR
                     KLLPALRHLHAALDAKANAWDGIIKIGRTHTQDATPVTLGQEFSGYAQQLSNGIKRLE
                     LTLPMLMELAQGGTAVGTGLASPVGFAEAVAKEIADLTALPFTSAPNKFEALAAHDAM
                     VFTHGAITTVAMSCFKIANDIRFLGSGPRSGLGELALPENEPGSSIMPGKVNPTQCEA
                     MTQVCAHIHGNNAAIAFAGSQGHFELNVFNPMMAYNFLQSVQLLADAAISFTDNCVVG
                     IEPRLDNIAKGLANSLMLVTPLKEKYGYDRAAKIAKTAHKNGTTLREEAVKDGIPAED
                     FDAIVRPELMIGPG"
     gene            complement(9923..10411)
                     /locus_tag="PK812_02005"
     CDS             complement(9923..10411)
                     /locus_tag="PK812_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2938 family protein"
                     /protein_id="HRJ68354.1"
                     /translation="MSDFKIQAIMLGIFATATLDVWNVALNRFAGMPVPNWGFVGRWF
                     AHMLRGRFRHEAIAAAEPVPNEVAIGWLAHYLIGILFAAILLAFWPGWIRNPTFWPPM
                     VVGWVTILAGWLLLSPGLGGGIAHARRPDATRARLLNIAGHTIFGLALWVGGMAILAL
                     GR"
     gene            complement(10441..11448)
                     /locus_tag="PK812_02010"
     CDS             complement(10441..11448)
                     /locus_tag="PK812_02010"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRJ68355.1"
                     /translation="MLQFPFDKPEDGAPVEIADGLHWVRFTIPYVLNHVNIYLLRDGD
                     GWLAIDTCIDYPVARHLWTTLLERFSPLNRLLLSHFHIDHTGAAGWLHEQTGCQILMS
                     PVEYALMERFRAPWSAELIESMRTNYLRMGCGAQEADELAHARWVPTRHSGSTPRLDV
                     PLLRGDEVTIGKRRWRVMVGRGHSPDGTLLYCADERLLVAGDTILPSISPFIGVDHRD
                     PRANPLQDYLDSLQDIEGELHDDALVLPGHGLPFRGVRERLQALHTHHEERNERILAA
                     CRDSAKTVREIALAIFNRNLDGVMGMAMSETMAHVNWLIAQQRLSSTSHDGQLRLKTT
                     T"
     gene            11559..13205
                     /locus_tag="PK812_02015"
     CDS             11559..13205
                     /locus_tag="PK812_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006726860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding dehydrogenase"
                     /protein_id="HRJ68356.1"
                     /translation="MDKADVIVVGGGLAGLVAAAELGDRGKSVILVEQEGENSLGGQA
                     FWSLGGLFFVNSPEQRRFGIRDSRELALRDWLGSAQFDRPEDEWPRRWAEAYVDFAAG
                     EKRSWLAGQGVKWFPMPGWAERGGGFASGHGNSVPRFHLTWGTGPGIVEPFERRVRAH
                     VADGRIVLKCRHQVSRIVMTNGRAAGVAGERLEESRVGRGQQSGRGIVGDFEFSADSV
                     IVSSGGIGGNFDLVRKSWPVERLGPAPKHMVAGVPHHVDGRMIGITQRAGGAVINADR
                     MWHYTEGLHNWDPIWPNHGIRIIPGPSSMWFDARGERLPAPCLPGFDSLGTLKAILAT
                     GHEYSWFVLTKAIAKKEYVLSGSEQNPDMTSKSWLSVLKSRRGRGAHPNVEAFLKHGK
                     DFVVRDDLRDLVAGMNALTGTDLIDHDRLKAQIEARDAQLDHAFSKDFQIMAIQNARR
                     YLGDKIVRTAKPHRFLDPKNGPLIAIRLNILTRKTLGGIHTDLDARVLDGSGAAIAGL
                     YAAGEASGFGGGGYHGYNALEGTFLGGCIFSGRVAGRAAG"
     gene            complement(13404..14012)
                     /locus_tag="PK812_02020"
     CDS             complement(13404..14012)
                     /locus_tag="PK812_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003597772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor
                     SspB"
                     /protein_id="HRJ68357.1"
                     /translation="MSTDLIRYDVLVQQALRGVVRKVLTDAAKEGLPGTHHFYISFNT
                     DAPGAKVSARLKSMYPDEMTIVLQHQFWDLSVSDTGFEVSLSFDKIAERLVVPFEAVT
                     GFFDPSVKFGLKFEAAEVAPENDDVATKPAAKGKGKAAKKPDPEAEAPPEPARIGKKP
                     AKAPAAETKSEPPAEKHPDDSEKPTAPGTAEVVSLDAFRKKK"
     gene            complement(14103..14463)
                     /gene="ssrA"
                     /locus_tag="PK812_02025"
     tmRNA           complement(14103..14463)
                     /gene="ssrA"
                     /locus_tag="PK812_02025"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01849"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="alphaproteobacterial-type; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01849"
     gene            14563..14892
                     /locus_tag="PK812_02030"
     CDS             14563..14892
                     /locus_tag="PK812_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2853 family protein"
                     /protein_id="HRJ68358.1"
                     /translation="MELKDHIADIKKYTASVDEKAVAGMAKTYALVMSKADSRYVAVS
                     DPDEVARVVEGFCKKKLGRKEKDADLVAVVKAQGEKMKGDRTKSRITIYYLIAEHFKQ
                     LSAFHPK"
     gene            14964..15758
                     /locus_tag="PK812_02035"
     CDS             14964..15758
                     /locus_tag="PK812_02035"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HRJ68359.1"
                     /translation="MKAYLDLMRLVLETGVDKADRTGTGTRSLFGAQMRFNLADGFPL
                     VTTKRLHLKSIIHELIWFLKGDTNVEYLQERGVSIWDEWADENGDLGPVYGKQWRNWD
                     SPDGRHIDQIREVIARIRKDPDSRRLIVTAWNPADIPFMALSPCHCLFQFYVAEGRLS
                     CQLYQRSADLFLGVPFNIASYALLTHMVAQVTGLKVGDFVHTFGDVHLYANHLEQARL
                     QLTREPKSLPRLWLDPSVTNIDDFRFEHIRIDNYLFHPAIKAPVAV"
     gene            15822..16040
                     /locus_tag="PK812_02040"
     CDS             15822..16040
                     /locus_tag="PK812_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68360.1"
                     /translation="MRVAAIAVLALVLPALGGCKMALLKGATRLALGAEQAETLDPLA
                     MVRRQLRSPPPRAPSPADASSRPPDPAR"
     gene            16045..16557
                     /locus_tag="PK812_02045"
     CDS             16045..16557
                     /locus_tag="PK812_02045"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HRJ68361.1"
                     /translation="MTTLVMVAAVARNGAIGRDNRLLWRLKSDLQRFKRITLGKPMIM
                     GRKTYDSIGRPLPGRTTIVVTRDPAFAAAGVMVAASPEAALHLARQEAEKLGVPDVII
                     AGGGEIYTRFLPIADRLEITEVDLAPVADAYFPPLDPTLWKVAAREAHAPGPGDETAF
                     TFVTYVRADA"
     gene            16690..17826
                     /gene="hflK"
                     /locus_tag="PK812_02050"
     CDS             16690..17826
                     /gene="hflK"
                     /locus_tag="PK812_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014286579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HRJ68362.1"
                     /translation="MPWSNNTGGGGPWQPKNNGPWGGGPQGGGGGGGGGGNRPPDLED
                     IFRKGQDRLKSIVPGGGGVGGIGVALIAVAGVLVWLATGIYTVRPNEIGIERVFGQFR
                     NVAPPGLHYNWPAPIGRVDKPVIAVQSVEIGMRTEMGRGGLRRDVPQESLMLTGDENI
                     VDIDFTVLWRVDERKPQDFIFNMRNTEATVKAVAEAAMREVIGKSNVIPVLTGARQTT
                     ESAVQELMQKVLNEYGAGVTVQQVQLQKVDPPAQVIDAFRDVQAARADQERAQNEAQA
                     YANKVVPEARGEAARIIQRAEAEKEKMIAGARGEAAQFVKLYDEYKKAPEVTRQRLFL
                     ETIERIYGGMDKVILDQSGAGQGVVPYLPLDQLNRRSGTQGAPR"
     gene            17826..18725
                     /gene="hflC"
                     /locus_tag="PK812_02055"
     CDS             17826..18725
                     /gene="hflC"
                     /locus_tag="PK812_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HRJ68363.1"
                     /translation="MALHPIIAGVVGVAAAAAFTSVFVVDQTQQAIVTQFGKPQRVVV
                     KPGLNFKAPFFFESVIHLDKRILDLESASEEVITSDQKRLVVDAFSRYRIENPLQFFQ
                     ATSGSIAAGEDRLRTLLNSSLRRVLGESSFAAVVRDERAGLVLKVKEQLNREAQALGI
                     GVVDVRIRRADLPEQNSQAVFRRMQTERQREASEYRAQGVEASERIKARADRDVVVTR
                     AEAERQARETLGSGDAERTKLLADAYGRDGEFFAFWRSMQAYEAGLRAGDTKMILTPD
                     SDFFRYLNDPKGRARAPNGEKKQ"
     gene            18756..18935
                     /locus_tag="PK812_02060"
     CDS             18756..18935
                     /locus_tag="PK812_02060"
                     /inference="COORDINATES: protein motif:HMM:NF021366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HRJ68364.1"
                     /translation="MVFAVALVLAFEGIVCAAFPSAMKRAMQQAGHLPEGAMRIAGLA
                     SAVVGVALLWLIKAA"
     gene            19047..20561
                     /locus_tag="PK812_02065"
     CDS             19047..20561
                     /locus_tag="PK812_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HRJ68365.1"
                     /translation="MSTLPMRSVRGTLANRASRATRAVATIALGASLFLTQVPAALAR
                     GPESLADLAASVMDAVVNIKASQTVQQRSVPMPQLPPGSPFEDLFNDFFNRRGPGGEG
                     QQGRPQQRSSSLGSGFVIDASGIVVTNNHVIADANEVTVVFNDGTELKAEVVGKDSKV
                     DLAVLRVKTDKPLKAVKWGDSQKARVGDWVMAIGNPFGFGGSVSAGIVSAKDRNIRSG
                     PYDNFIQTDAAINKGNSGGPLFNMAGDVIGVNTAIISPTGGSIGIGFAVPSAMAMGIV
                     DQLTKFGETRRGWLGVAIQDVDDAMAESLGIGKARGAFVSSVDDKGPAKPAGIEREDV
                     IIKFDGKDVRMSRDLPRIVAETPVDKQVDVVILRGGKEVIRQVKIGRLEDNDRRASAA
                     PGNAPSSGNNSNASRTVIGLDLSPLNPDLRRRFNIKDSVNGVVITRVAPGSRAAERMI
                     QPGDVIVEVQRTPVKTVAEVSSRLDELKKEKRANALFYVANANGDLRYITLPVE"
     gene            complement(20676..21572)
                     /gene="serB"
                     /locus_tag="PK812_02070"
     CDS             complement(20676..21572)
                     /gene="serB"
                     /locus_tag="PK812_02070"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014765103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HRJ68366.1"
                     /translation="MLFVATLIANPSTPVLEEAHLRAAAQGLPDADAAKWLAPGIAAD
                     LTFEADNHFDCRGAALAVQLMLAGLPADVVVQPAATRRKRLLVADMDSTMIGQECIDE
                     LADFVGLKDKVSQITERAMRGEIAFEPALRERVALLKGLPATIADRIIAERITLTPGG
                     AELVRTMRRDGGEAWLVSGGFTLFTRKIGASIGFTADRSNELVLDQGLLAGRVAEPIL
                     GKEAKRLTLIEVRERLGLAAHETLAVGDGANDLDMIREAGLGVAFRAKPAVAAEAHAR
                     IEHADLTALLYAQGYRRDEFAL"
     gene            21571..22524
                     /gene="miaA"
                     /locus_tag="PK812_02075"
     CDS             21571..22524
                     /gene="miaA"
                     /locus_tag="PK812_02075"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HRJ68367.1"
                     /translation="MEPAEALSEIARRCRAGALDAVLIAGPTASGKSALALALAETLG
                     GTVVNADSMQVYADLRVLSARPTRDEEARVPHRLYGIVPAARAYSVGDYLRDAAPVLA
                     DLKRAGRVAVIVGGTGLYFRALTTGLAETPVVPQEVRERVEGLARHGADLHAELARRD
                     PEAAARLSPADSPRIQRALEVFEATGTPISRWWQSHQSVPLLAPGRWQGLFLNPSRDA
                     LFARIDARFREMMGQGALEEVRHLATLGLAANRGVMKAHGLPHLVAHLAGALDLDEAI
                     RLGQRDTRRYAKRQLTWARKFMPDWIWAESVPALGGLVPPA"
     gene            complement(22531..23928)
                     /locus_tag="PK812_02080"
     CDS             complement(22531..23928)
                     /locus_tag="PK812_02080"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HRJ68368.1"
                     /translation="MYVSDIEDHLRTPESRELLARYRQAQRAGGLPHVRLIDSVTMRT
                     IGRDLTLVEKNAHGEFEYLYFGEALQRLTGSDLTGKSTAHQSPQSGASARRTFLKTLE
                     LRAPLFAIDREAENGRVHLWGRLHLPLDDGRGGIVICSYNRPREYSDELLREILDAAA
                     DGILAVRATRDGHGNVIDGAVLAANAPMLEMLRREQQDVLATPVSRLFPELRREGLWE
                     QHVEVLQTRQCLSYEAVLERGGTARWYRIVAAPMNDGLVISFTDITELKQLNMMLEEQ
                     RKRLTEEMERRTLVEQELWNLAHLDPLTNVANRRSMKEQSIATLKLAARNEVSCAMIV
                     IDIDYFKRINDTYGHSAGDKAIQAVATAAQSCCRSQRDILARTGGEEFAMLLYDANAT
                     TAIAIAERLRQRIEQMTISDGANAFTVTISCGVAESREGLGYEEFLVAADRALYSAKN
                     DGRNRTHTEEGRKAA"
     gene            24282..26042
                     /locus_tag="PK812_02085"
     CDS             24282..26042
                     /locus_tag="PK812_02085"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HRJ68369.1"
                     /translation="MSETMTGAEMVVRALQDQGVEHLFGYPGGAVLPIYDALFQQDKV
                     QHILVRHEQGAAHAAEGYARSTGKVGCVLVTSGPGATNAVTGLTDALMDSIPLICITG
                     QVPTHLIGSDAFQEADTVGITRHCTKHNYLVKRVEDLPRILHEAFYVAANGRPGPVVI
                     DIPKDVQFARGKYSRPTNSEHKTYRPRVDGDMALIERAVDLMAKAKRPIFYTGGGVIN
                     SGPAASAALRDLVRATGFPITSTLMGLGAYPAADPQWLGMLGMHGTYEANLAMHDCDV
                     MINIGARFDDRITGRIDAFSPKSKKIHVDIDPSSINKNVKVDIGIIGDCGRVLVEMVR
                     IWKEKKHKADAEMLKAWWGQISKWRARNCLAYKRSETEIKPQYAVERLYALTKGRDAY
                     FTTEVGQHQMWAAQFLHFEEPNRWLTSGGLGTMGYGLPAAVGAQLAHPKSLVVCVAGE
                     ASVLMNMQEMSTAAQYRLPVKIFILNNEYMGMVRQWQELLHGGRYSESYSAALPDFVK
                     LAEAYHAKGIRCSDPKELDAKIQEMIDYPGPVIFDCLVTKEENCFPMIPSGKAHNEMI
                     LPDFEGDTGQIIDAKGKQLV"
     gene            26045..27022
                     /locus_tag="PK812_02090"
     CDS             26045..27022
                     /locus_tag="PK812_02090"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine dehydratase"
                     /protein_id="HRJ68370.1"
                     /translation="MRPTLADLEQAHAVVRAAMPPTPQYAWPLLGQRLGAEVVVKHEN
                     HTPTGAFKVRGGLVYMDRLKRERPQVAGVVSATRGNHGQSLAFAGGRHGVAVTIVVPR
                     GNSIEKNAAMRAFRADLIEHGSDFEEARQEARRLAENRGLEMVPSFHPDLMCGVATYA
                     LELLTAHPDLDVLYVPIGQGSGICGCIAARDALGLRTEIVGVQSTEAPAYALSMAAGH
                     AVSTNSANTKADGMATRVPDGEALAIILRGAARIVTVTDDEIAVATRAYWTDTHNIAE
                     GAGAAALAAAMQETTRLAGRRVGLILSGGNIDFDLFRTWICGVPASQPI"
     gene            27036..27581
                     /gene="ilvN"
                     /locus_tag="PK812_02095"
     CDS             27036..27581
                     /gene="ilvN"
                     /locus_tag="PK812_02095"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HRJ68371.1"
                     /translation="MKSHYSDPSRHQSVERRTLAIIVDNEPGVLARIAGMFSGRGYNI
                     ESLTVSETEHEKHLSRITVVTRGTPEVIDQIKAQLDRLVPVHRVVDLTLSGSAIEREL
                     ALVKVAQEGATRSEHRMEALRLATAFNARTVDATLGSFIFEIAGTSDDVERFVSLLGQ
                     IGLVEVARTGIAAITRGAGGL"
     gene            27582..28190
                     /locus_tag="PK812_02100"
     CDS             27582..28190
                     /locus_tag="PK812_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018095390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HRJ68372.1"
                     /translation="MSASATLAALLVFSFVNSVTPGPNNLMLLASGVNFGFRRTIPHM
                     LGIGCGFMVMTALVGVGLSQVFERFPIAYLALKVAGAGYMVWLAWKIATSGPVGEGTS
                     RGKPMTFLQAAAFQWVNPKAWMMVVTAVSTYSITDSVVVNALTIALVFGIVNPPSISV
                     WVLFGTAMQRWLRDPVRLRLFNGCMAAALIVSLIPMLLDGKP"
     gene            28290..29309
                     /gene="ilvC"
                     /locus_tag="PK812_02105"
     CDS             28290..29309
                     /gene="ilvC"
                     /locus_tag="PK812_02105"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018324362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HRJ68373.1"
                     /translation="MRVYYDRDADLNLIKGKKVCIVGYGSQGRAHALNLKESGVKNIV
                     IALKPTSATAEKVKADGLKVMSVAEAAKWADVMMMATPDELQADIYNTEIAPNIRDGA
                     AIAFAHGLNVHFNLIEPKKSLDVIMIAPKGPGHTVRGEYQKGGGVPCLIAIHQDASGN
                     AHDIALSYACGVGGGRSGVIETNFKEECETDLFGEQVVLCGGLVELIRAGYETLVEAG
                     YAPEMAYFECLHEVKLIVDLIYEGGIANMNYSISNTAEFGEYVTGPRIITDETRAEMR
                     RVLKDIQTGAFTSEWIRECRAGQPKFKATRRMNDAHPIEEVGSKLRAMMPWIAKNKLV
                     DKARN"
     gene            29429..30574
                     /locus_tag="PK812_02110"
     CDS             29429..30574
                     /locus_tag="PK812_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3095 domain-containing protein"
                     /protein_id="HRJ68374.1"
                     /translation="MTRFYDDLPREADFGALLEPAAYVPLPHDWLVGVTDVVDSTGAI
                     AAGRYKSVNMAGATVIAALTNAFPGRSFPFVFGGDGASFAIPGTQESAARAVLGQTIR
                     FVEEALGLALRAALIPVSDLRAAGKDVAVARHAASASIAYAMFRGGGLSHAEAEMKRG
                     AYAVAPSAPGDAPDLTGLSCRWSPIPARHGVIVSLILSPAHGEDAEFRAVVGKVLGLA
                     SEAGQAARPVALAEDISVRWPPDGAVNEALTRPVWRRAPALARLYLRGLIAFQKLIFA
                     RNWTVGAFDPARYRRDLVANADFRKYDDGLKLTLDCTPALADRLEALLAEAERSGVAR
                     FGLHRQGAAIVTCFVPSAARNDHVHFIDGADGGYARAAHRLKAKQAA"
     gene            complement(30582..31739)
                     /locus_tag="PK812_02115"
     CDS             complement(30582..31739)
                     /locus_tag="PK812_02115"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRJ68375.1"
                     /translation="MSRRNTIRVATFNVENLFTRLAFPDAMPPEDNLVGAYQFDNPEE
                     LKLARRIVEAAASDDKRQLTALALHACRADIVALQEVDSKESLEVFYQRYVKRMIEPE
                     AAAEKNELRREARRAGRRPEDSDLFAVDRRHYYDWRVVLDGNDGRGIDVGVMSKVPIR
                     VRTHAHRTFQELGVWNEDLKNYRERINGRERRLTQNDRLFRRDCLEIDLEVGSRPLTL
                     YVCHLKSMNPSRELTRPMRKAEALGIRRLIEDRFGRHARDANWIICGDMNDFTEVDGL
                     PLLDDTGKVAPHGLEPLFADGFSENIVRRREPDERWTTYHGPEDQYAQLDMLLVSPAL
                     AKANPDAVPDIIRMGQPYRARRYEGPRFPRIGWDRPKASDHCPVVVELAIP"
     gene            31889..32713
                     /locus_tag="PK812_02120"
     CDS             31889..32713
                     /locus_tag="PK812_02120"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ68376.1"
                     /translation="MASNQNNTAEPAIRFDGVSISYVLADGGRYDAVAPVDISIADGA
                     FVSIVGPTGCGKSTLLNVAAGLAAPTTGSATIFGKPLAGINAQAGYLFQQDALMPWKT
                     AQDNVALGLEVQGLPRAQALAKAHDMLRRVGLKGFGERYPHQLSGGQKKRVALAQVLV
                     RGPKILLMDEPFGPLDAQTRLIMGELLSGLWQADRKAVIFVTHDLDEAIGLSDRVLIM
                     AAGPQSRIIGDFEIGLERPRDLAEIRSDSRFHTLHAEIWAVLRDEVRKTYRDVEAA"
     gene            32713..33504
                     /locus_tag="PK812_02125"
     CDS             32713..33504
                     /locus_tag="PK812_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ68377.1"
                     /translation="MSRAKLLVLQVGVAVIAIAIWHVVTTTNLIGDQKVMKFFFSTPA
                     DVFWRTWKVFAEGTIWRHLGITLWETILAFVIGSAAGIVIGFWFARQPLVAAVFDPYV
                     KMINALPRVVLAPIFALWFGLGIWSKVALGFTLVFFVVFFNVYQGVKEVNPVVLANAR
                     MLGMNERQLLRHVYWPAALSWVFSSLHTSVGFALVGAVVGEYLGSSAGLGYLIHQAEG
                     VFDVAGVFSGMLILAVFVVIIDGLVTMIEGRLLKWRPSTEIKTQA"
     gene            33533..34549
                     /locus_tag="PK812_02130"
     CDS             33533..34549
                     /locus_tag="PK812_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012092984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRJ68378.1"
                     /translation="MRISRRGLLVAGAGLVAMPGIVRAQSVEKPKLVLGVGGKNLLYY
                     LPLTIAERRGFFKEQGLDVEITDFGGGAKALQSLVGGSADIVTGAYEHTIRMQAKGID
                     IRSTLELGRFPAIVVAVKKDLAGKVKSAADLKGLKIGVTAPGSSTNMLVQFAMVKAGL
                     KPMDAAWVGVGAGASAVAAMKKGEIDAISNLDPVISKLEADGDIVVLIDTRTEAGTQA
                     LFGGTNPAACLYMKNDFIEKNPETTQRLVNGLFKALKWIGSASADDIANSVPAEYHLG
                     DKGIYMRSLAAVRDSYSKNGIVSEAGHQSIHAMLVQLDAELKDAKIGRDKTFVERFVA
                     KASA"
     gene            34616..35485
                     /gene="pdxY"
                     /locus_tag="PK812_02135"
     CDS             34616..35485
                     /gene="pdxY"
                     /locus_tag="PK812_02135"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: protein motif:HMM:TIGR00687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HRJ68379.1"
                     /translation="MPRVLSIQSHVADGHVGNAAAVLPMQRLGIEVLPVHTLQLSNHP
                     GHGAFRGRVFEASHVAEVLEGLEAHGSLASCDAVLTGYLGSAAIAEVVRDVVARARSL
                     NPSLVYVCDPVIGDAHKGVYVRPDLADAIRDRLVPLADFLLPNQFELQFLTGIDICDA
                     ASLRAAAERLTGGVRSAVVTSVTHGGIAAPCVATALFDARGTEVIETRRIDRQFSGAG
                     DCFAALFTAYRLVTGDNRAAHWQAATAMASLLSKTERLGRDELALVEGQGAFRLRSLA
                     ELSRAHRQWKRPG"
     gene            complement(35576..>35797)
                     /locus_tag="PK812_02140"
     CDS             complement(35576..>35797)
                     /locus_tag="PK812_02140"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRJ68380.1"
                     /translation="TAPAPAAQPAAQNQLVNLNTAPASELDKLPQIGEKRAADIIAKR
                     PYKDWNDFVAKNVVPSNAEAAIKDKVRFR"
BASE COUNT         6074 a        11272 c        12044 g         6407 t
ORIGIN      
        1 gaaggcgccg gctcgtcggc cggatcgaag gcggggaagg acaaggtctg tctcgcgaat
       61 ccgaaatagc ctagcttaga gcatcgggcc gggcatgaga acgggacgcg aaccggaacg
      121 ctggcgtcca cacatcgttc accatgacct gctcgaatgg cgaatcagag gcgagtgaca
      181 tggccaaccc gaacgatcca tcgacgcaac gcccggcccc gggggcagtc tcagcggcca
      241 ttgcagagct gacgcgcact ttcggcaatc gctgcgtcac caatcagtcc gtgcgcgaca
      301 atcacgccca tacgcgcacc tacctgccgc cgcagccacc ggacgccgtc gttttcccgc
      361 acgacgaagg cgaggtcgcg gcagcggtct ccatcgcggc ccggcatggg cttccgatca
      421 ttcctttcgg catcggctcc tcgctcgaag ggcaggtgaa cgccccgcta ggcggcctgt
      481 gcatcgatac cggacagatg aaggcgatcg tcgcggtgca tccggaggat ctggacgtgg
      541 tggtccagcc cggcgtgacc cgcagcgagc tgaatgcgca tctgcgcgat ctcggcctgt
      601 tcttcccggt cgatcccggc gcggatgcga cgctgggcgg catggtggcg acgcgcgcct
      661 ccggaacgaa cgcggtccgt tacggcacca tgaaggattt ggtcctggcg ctccgcgtag
      721 tgctggcgaa cggcgagatc gtgacgacag cgcggcgcgc caagaagacc agcgccggct
      781 atgatctcac ccggctcttc gtcggttccg agggcacgct cggcgtcatc accgaaatca
      841 cgctgcgcct gttcggcatt ccggaggcga tctcggccgc aacctgttcg ttcccgagcg
      901 tcaaggcggc cgcagatgcg gccagcctgg cgattcagtc cggcattccc ctggctcgcg
      961 tcgagctcat ggacgccatg gccgtgaagg ccgtcaacgg ctattccaag ctctccctgc
     1021 cggaacagcc gctgctcctg cttgagtttc atggatcgcc ggccggcgtg aaggaacagg
     1081 cggaaagttt tcaggccatc gccgaggaat gcgggggagc cggtttcgcc tggacggcgc
     1141 ggccggagga gcgctcaaag ctctggcagg cgcgccatga cgcctactgg gcgggcctga
     1201 cgctgaagcc cggcgcaagc tgtctttcca ccgatgtgtg cgtgccgatc tcgcgcctgg
     1261 cggaatgcat cgatcagacg gcggctgatc ttgaggcgct ccggattccc ggaaccatcg
     1321 tcggccatgt cggcgacggc aatttccatg tgctgccgtt gttcgacccc aacattcccg
     1381 aggagctggg ccgggtgaag cagttctgtg accggctcgt cctgcgtgcc ctggcgatgg
     1441 aaggcacctg caccggcgag catggagtcg gacagggcaa gatgaagtat ctcgacactg
     1501 aactgggcgc gccggcgctt ggggcgatgc gggcgatcaa gcaggcgctt gatccccgaa
     1561 atctcatgaa tcccggcaag gtggtcacgg tttagccaag gagcgatggc aggaaggccg
     1621 ctggccttcg gacagaacga ggtgacggca cagtgcgtga gttcctgact ttcatcggca
     1681 ttgttctgtg cctggcgctg gcggcgctga tggccgggcc gcattttgtc gattgggacc
     1741 actaccggcc acagatcgag aaccggttgg cggcgagcct gggaatgccg gtttcggtga
     1801 acggcccggt gtcggtgacg ttgctgccca ccccttcgct cactctggag aaagtgcgtt
     1861 tcggcagcgc ggatggcggg aaggccagca ccttcgaggc ggatcgcctg gcggtcgtgg
     1921 tttccgcgac ttcgctgttg cgcggtgaaa tccacgtcac cgaagccctg atcgacgcgc
     1981 ccgtattgaa ggtgcgccag ggggcgccgt tgctttcgat gtcggggtcg ggcggtgtgg
     2041 tacggccgga acagatcagc atcgagagcc tgcggctaag ggacgggaag atcgtcgtcg
     2101 aacgggacgg tcagcccgac ctccggatca cgggcttgag cgccgatctg gaagccaaca
     2161 gcctcatcgg cccggccaag ggcagcggct cctttgttgt cggaacaacg ccgcgcacgt
     2221 tccgctttgc gctcggcaag ctggaggaag gccggtcgcg gctaaaggcg ctcgtggaag
     2281 atgcgcagct ggcgctgagg ctcgatgcgg atggggtgct cggattggtc ggagcgggcg
     2341 ccggcttcga gggtagcctg cacttgtccg gaaatccgac gctcggttcc gatggcaaag
     2401 gcccgcaact gccagttcgc gtgtccacgc gcagccggat tgatggcacg cgcgccgtat
     2461 tcgatgacgt ttcgatggtg attggcgccg aaccccagcc gctgacgctt accggcaatg
     2521 cgggcatgac ttttgacggg cgcagacgtg tcgaggcaac gctttcggcc cgctcgttcg
     2581 acttcgaccg ccccggtccg gacggaaagc cgcgtgcggc cgtgccggtc gacctgatcc
     2641 ggcaggctgc ggcgctggtt ccgggcggca cgggcgttga agcaccggac ttcgaccttg
     2701 atgtctccgt cgggggcatt atcgtcggcg gccagaccat cgtgggtggc agggtggcca
     2761 tcagcaagga tgcgagcggg atcgggatcg aagccctcga aggcgagttg cccgggcaaa
     2821 cccggatcaa gctggcccgg tcggcaaaat ctcccgccgg attgctcgcc gggcgcatcg
     2881 aggccgaatc ccgcgacccg gaacggttcc tgcgctggct gacgggagaa cctggcgttg
     2941 cgacatcggg gctttcgctg aaagcgtcgg cggaactgga aacgtccgtg gatggcttcc
     3001 gtctccgtga aatcggcatc gaacggggcg atacacggct gagcggcgag ggcagtttcc
     3061 tgctcgccgt gccaggtcag cgcccggttc ccagggtcgt cctcaagctg gccagcccga
     3121 gactgaacat cgtcgatgtt cccggactga cgttcggaga gaaatcggga gaaagccccg
     3181 acatcgatct cgatttcgag gtcgaggcca ccaagctggt gttcgaaggc aacgagaccg
     3241 gtcgcctgat ggcccggatt cggcgcgatg gcccgatcgt atccatcgag cggcttgccg
     3301 tgaccgaatt cggcggcgcc agtctgatcg catcaggcac cattggtgga ggcgcgcgcc
     3361 gcgtcacggt caagctggat gcgaaccggg tggaggcgct gaccgcgctt ggcgaaaagc
     3421 tcgtgcccgg cgcgttcatg gcgtcggtgc gccatcggtc gccgctgctg gcgccggctc
     3481 tggttgtcgc cacctttgcc aatgacgacg ccgacgattc ctacgacatc cgggccgacg
     3541 ggcatttggc aggcaccaag gtgcaggccg caggaaagct ttccgggcgc acggacctgc
     3601 gtctggatct cgaactcggg ctggaaagcg ccgatggcgc gttgctggcg gcgcagatcg
     3661 gcggccatcg atcgccagct gtgggcgcag gtccggcgaa gatcgacctg tcaatgcgtg
     3721 gcgatccgcg cggttccctg tcgacggtcc tgaccggcag catgcccggt gtcgatgcac
     3781 gcgtgatcgg caatatccgg atatttcagc ccttcgcgcc ttttgccggc gacctgacga
     3841 tccgagcggc ggatctcgga ccggttgcag ccgcccttgg cgtgacacat ccagccattc
     3901 caaaagggac cacggccact ctctccgggc atgtggaaag caacctggag cggatcacga
     3961 tcgagaatct caaggcccag atcggcggac aggagtattc gggcgagatt gccttcagtc
     4021 tggccgagaa tggcaaggtc gccggccaga tcaagacacc ctatgtcgac ttccgcccgg
     4081 tgatcgggct cgcgctcggg gcgcctaggg ctggcgccgc cccctgggcg agcactgcct
     4141 ttgccgatcc catggcgccc cccttgccgg gcgatctttg gatcgaggcg gaccgggcgc
     4201 ggctggatga caagaccctg ctcaacgacg cgaagttcgt tttacggttt gatcgcgggg
     4261 cggcgagcgt cgagtatgcg cagttcgcgc tcggaccggc gcgtgtcgag ggcgatgtgt
     4321 tcctgcgccg cacggccgga accgcgttcc tctcaagcaa gctgaaggca agcggcatcc
     4381 gtctcgaaca gatgggcgac ggctcggcga agggcctgct tgacgttcag gcccagttct
     4441 cgggaagcgg cgagtcgccc gacaagctga tcgccgcgct ggctggcggc ggcgcgctga
     4501 ccatgcgcca ggtgcaggcg ccacgttacg atcccgatgc gctcggccgg ctgcttgcca
     4561 tgccgctgga caagctgggt ccgctcgacg cggccaattt gacccgtcgg cttgaagccg
     4621 aactggaacg gtcccactgg accgttgcgg aaacccgggt gcctatcgtc gtggtgaacg
     4681 gcgtggtgcg gactggggca acggcgattg acagcaaaga tgccaggcac gaggcgcagg
     4741 ctgcgcttga tcttggtggg atggtactgg acgtcaaggt caccacctcg tcggctgctg
     4801 ttccagcgga ttggcgcggc gcgcatccgc agttctcgac gcagtggcgc ggcccggtcc
     4861 ccaatctgcg gcggagcctg gcggtcgatt ccctggtcaa cggctacctg tctttggcgc
     4921 tggtgcgcga tctccagaag accgaagtct tcgaacagga cgtgcgcgag cggtccgcgc
     4981 acaatcgccg gctgaaggcc gatgaggcca tgcgccggcg ggccgacgaa ctcagtcggt
     5041 tcgagcagat cgaacgcgag cgtcgtgaga aggcggcgga agaacagcgc cagcttgagg
     5101 cccgccttgc ggaggatgcc cggcgcagag ccgaggaaga ggaacgcgcg cgcgccgaga
     5161 aggcggctga ggccgagcgt caacgggcgg ccgaagagga aagtcagcga caggcgcggc
     5221 tggctgaaga ggaggctgcg cgcattcagc gaatccagcg acttctgcaa aaccccgaga
     5281 cgatggctac gccgacgccc cgccgcagcg tggcgccgca acctcagcag gcgcccctgg
     5341 caccaccgct cagtctcaat ccgccaacgc ctggacgtcc aagggtggcg gcgccagttg
     5401 aaatcaggcc gttgaacgct ccttctgcgg cgccggatac ggcgcgctga gcaggccagc
     5461 cttcgccgca tcaagaacat ggacgcgcag agcggacgac agatttgtgt cgcgccgctc
     5521 accgtcgact tccgcgatca gcgtcgctgc ggacacactg cgccgggcgg caagcacctt
     5581 caggtcgttc cagaaactct cttcgagcga gatggatgtg cggtggcctg cgatggagac
     5641 cgagcgcttg atgacccgac cgctcacaga tccggatcca gttcgcgctt gtgtccttcc
     5701 agccgggtgg cctgccggtc gcgctcctgt ttcaggcgct ccctgtcagc cttgggcagg
     5761 ccgaagcgga tacggttctc attggcgcgc gcctcctgct cggtacgcag acgttgcttg
     5821 cgggcacgac gcaggttgat gatctcggcc gacatctttt cccccatgat ggccgccgcc
     5881 agcatatgcg ctgcggtggc cggtgcaaag ggcggctcag gcgctcttgc gcatcaggtt
     5941 ggcgaggctc agggtcgccg actgcggctt gagttgcggc cagtcggaca aggcgctcgg
     6001 cttgccgatg aaatacccct gtacgctgtc gcagccaaga aaacgcaggc ggtcgagttg
     6061 ttcctcggtc tcgatgcctt cggctatgac ctcgatgccg aggccgcgac cgaggccgat
     6121 gacggcattg accaccgcgc cgtcggcctt gtccgcacca ccaccgctga taaacgagcg
     6181 atcgatcttg atgcgatcga attcatactg gcgcagatag cccagtgacg aatgtcccgc
     6241 gccgaagtcg tcaagagcga tgcggatacc gaatccgcgc agcgccttca gcgcggcgcg
     6301 cgttatggcg ttgtcggaga tcaggacgga ttcggtgatc tccagcgtca ggcgctgcgg
     6361 cgccagaccg gtgtccgaca ggcacttcag gatcgaggtg acgaagttcg actggcgcag
     6421 ctgcacggcg gatacgttca cagacacgga caacggcgca ctgacagccg cgaagtccgc
     6481 gcaggcctgg ttcaggaccc agttgccgat gcgattgatg agttcgcttt cctctgccac
     6541 cgggatgaaa tcgctggggg cgatcatgcc atgtgtcgga tgctgccagc ggatcagcgc
     6601 ttcgaagccc gaaacctcgc cggagcgcaa ggacagaatg ggctggtaga cgagcttgaa
     6661 ctcgccgccg gtcacggctg atcgaagatc gcgctcgatc cggcgtctct gctgcgcctc
     6721 ggcgtccatg ctggcatcga aaagccggac gcgtccgcct ccgttcttct tgacctgata
     6781 gagtgccaga tcggcgttct tgagcagcat gtctgtctgc ttcggatcaa tggcgccaaa
     6841 gcagatgccg atgcttgcgc cgatcgcgac agatcgtccg ccgatgtcga agtcttcgca
     6901 cagcgcggca accagagctt cggctttctc ctccgccgct tcggcatgga ttgccgaggg
     6961 aaggatcaca gcgaactcat cgccaccgag gcgggcgacg agcgcttcct ctcctgcact
     7021 ctgactcatc cggtcggctg ccgcccagag caagagatcg cctgcggtat ggccgtaact
     7081 gtcgttgatt tccttgaatc ggtcgagatc aatgcaaaga acggccagtg gctgatccgg
     7141 caacgtttcg gcgcgccttg caagccagtc catcagattg cggcggttgg ccaagccggt
     7201 cagcgcgtcg tgcatcgcca tgtgcacgat cttctgttcg ttcagctttc gctcggtgat
     7261 gtccgcgatg cacccttcgt agcagaccgc gttcccggca gcgtccctga ccagccaggc
     7321 cgtctcctgc gaccatatcc ggcggcccgt atcgacatgc tcgagttccg agacaaagtc
     7381 cgtgacacgg ccgagcctga acaggcgcct gcgaaacttc tctcgccggc ccgccttgac
     7441 gtagcgccat tcccggcggt cgttctgcgc tgccagcaat tcttccactg acccgtatcc
     7501 atggatccgt gcgaactccg gattggccgc agtcatctgc ccatcgagcg tggcgcgata
     7561 gatggcgacg gctgcgttct cgaacaggct ccgatgcagt tcctgcgcct cgcgggccgc
     7621 attctcttga cgccggcgct cggtgatgtc ggagaacgtc cgcagcgcgc cgccgccgtc
     7681 gagcaacagc gtgcggatct cgatgatcgt accgttcggg cgctggcgct cgtataccga
     7741 cggcgcggca cggacgtcgc ccttaccgac ccatccggtg gcctccttgt cgttaccgtc
     7801 gaactccccg atggaccatt ggtgcttgat caccgcagct atgggcgtgc cttcgcgcga
     7861 aaggtgttcc ggcagctcga gcaggtctgc cgcgcgctgg ttgcaggcga cgatgcggtc
     7921 ctcgtcgtca aagataatga tgccctgcgc cgtattctgt aggaccgcgc cgaacaggaa
     7981 ccgtgccgac ttcagttcgc ccgtgcggcg gttcacaagg tcttccagtg tgcgattgtc
     8041 gctcttcagc cgcttgaggg cgtaggcgac gctgcggtcg cgcgtgtcgg cattggtctt
     8101 gtagctgtcg gaaatcagat cgagaaagcg cgagatgtcg aacgtgcgat ccgcgcgcac
     8161 gcactctcgg acctggtgct caaggagcgg atgacgttgg gtcaagcaga atcgcgccgg
     8221 gaagaaaata cagattcgcc ccggaatcta ccgacaattg attaagcagg ccttgccgca
     8281 ttgcggccgc atttgggagt ggcggactgg agcttgtttt cgccccaggc gcgccacgtc
     8341 acggcgcgcc cggtacaggg ggcaacaagg cgctgtcccc ggactgaaga acaggcgctc
     8401 ctcagcccgg accgatcatc agttccggac ggacgatggc atcgaagtcc tcggccggga
     8461 tgccgtcctt caccgcttcc tctcgcaagg tcgtgccgtt cttgtgcgcg gtcttggcga
     8521 tctttgcggc gcggtcgtag ccgtacttct ccttgagcgg cgtcaccagc atgagcgagt
     8581 tggcgagccc cttcgcgatg ttgtcgagac gcggttcgat gccgacgacg caattgtcgg
     8641 tgaagctgat cgcggcgtcg gccagcaact gcaccgactg caggaagttg taggccatca
     8701 tcgggttgaa gacattcaac tcgaaatgtc cctgcgatcc ggcgaaggcg atggcggcgt
     8761 tgttgccgtg gatatgggcg cagacctgcg tcatcgcctc acactgggtc ggattgacct
     8821 tgcccggcat gatcgacgag cccggctcgt tctccggcag cgccagttcg ccaaggcccg
     8881 agcggggtcc cgaccccagg aagcggatgt cgttggcgat cttgaagcag ctcatggcca
     8941 ccgtggtgat ggcgccgtgc gtgaagacca tggcgtcgtg cgccgccagc gcctcgaact
     9001 tgttcggcgc cgaggtgaag ggcagggcgg tgagatccgc gatctccttc gccacggctt
     9061 ccgcgaagcc gaccggtgaa gcaaggccgg tgccgaccgc cgtgccgccc tgcgccagct
     9121 ccatcagcat gggcagggtc agttccaggc gcttgatccc gttcgaaagc tgctgggcgt
     9181 agccggaaaa ttcctgaccg agcgtgaccg gcgtcgcatc ctgcgtgtgc gtgcggccga
     9241 tcttgatgat gccgtcccag gcattcgcct tggcgtccag cgctgcatgg agatgtctca
     9301 gcgccgggag gagcttgcgc acgacttcct cggcgcaggc gatgtgcatg gccgtgggga
     9361 aggtgtcgtt ggacgactgg ctcatgttga cgtggtcgtt cggatgaacc ggcttcttgg
     9421 agcccatctc cccgcccatg atctcgatgg cgcggttgga gatcacctcg tttgcgttca
     9481 tgttcgactg cgtgccggaa ccggtctgcc agacgaccag gggaaaatga tcgttgagtt
     9541 tgccgtcgat cacctcctgc gaggcctgca cgatgaccgc gccgagcttc gtgtcgagct
     9601 tgccgagctt catgttggcc tgcgcggccg cgcgcttgac gatgccgagc gcgcgcacga
     9661 tcgaggccgg ctgtttctcc cagccgatct tgaagttgcc gagcgaacgc tccgcctgtg
     9721 cgccccaata gcgggtggaa tcgacctgga tggggccgaa ggtatcagtc tcggtgcggg
     9781 ttctcttctc ggtcatggca gggccttcgc tggctgaacg gtcacgaaac acgttcgcgc
     9841 ttctagcgaa gccgcgccgg ggcggcaatg cggcgaaggg ggcaggccga ctgtcaggag
     9901 gttaccgtcc cggcacagca gttcatctgc ccagagccag gatcgccatc ccgcccaccc
     9961 acagcgccag gccgaaaatc gtgtgccccg cgatgttcaa gagccgcgcg cgggtggcgt
    10021 cggggcgtct ggcatgcgcg atgccgccgc cgaggccggg agacagcagc agccatccgg
    10081 cgaggatcgt gacccagccg acgaccatcg gcggccagaa ggtcgggttc ctgatccagc
    10141 cgggccagaa ggccagaagg atcgcggcga acaggatgcc gatgaggtaa tgcgccagcc
    10201 agccgatggc gacctcattg ggcacaggtt cggccgccgc gatggcttcg tgccggaaac
    10261 ggccgcgcag catgtgcgcg aaccagcggc cgacgaagcc ccagttgggt accggcatcc
    10321 cggcgaagcg gttcagggct acgttccaga catccagggt cgccgtggcg aagatgccga
    10381 gcatgatcgc ctgaatcttg aaatcggaca tcacaccccc tctgcctcgt ggcctgacga
    10441 ctaggtcgtc gtctttagcc gcaactggcc atcgtggctt gtcgatgaca ggcgctgttg
    10501 cgcaatgagc cagttcacat gggccatggt ttccgacatg gccatgccca tcacgccgtc
    10561 cagattgcgg ttgaagatcg ccagcgcgat ctcgcgcacg gtctttgcgc tgtcgcggca
    10621 ggcggcaagg atgcgttcgt tgcgctcctc gtgatgggtg tgcagcgctt gcaagcgctc
    10681 gcgaacgccc ctgaacggca ggccatggcc cggaagcacg agggcgtcgt cgtgaagctc
    10741 gccttcgatg tcctgcaggg agtcgagata gtcctgaagc ggattggctc gaggatcgcg
    10801 gtgatcgacg ccgatgaagg gcgagatcga gggcaggatc gtgtcgccgg cgaccagcaa
    10861 ccgttcgtcg gcgcaataaa gcagggtgcc gtccggcgaa tggccgcggc cgaccatgac
    10921 gcgccagcgc cgtttgccga tcgtcacttc atcgccccgc aacagcggaa cgtcgagacg
    10981 tggcgtcgag ccggaatgac gcgtgggaac ccagcgggca tgggcgagtt cgtccgcctc
    11041 ctgcgcgccg caacccatgc gcagataatt ggtgcgcatg ctttcaatga gttccgcgct
    11101 ccagggcgcg cggaaccgct ccatcagcgc gtattcgacc ggcgacatca ggatctgaca
    11161 gcccgtttgc tcgtgcagcc agcctgccgc tccggtgtgg tcgatgtgga aatgactgag
    11221 cagcagccgg ttcagcggcg aaaagcgctc cagcaaggtt gtccagagat gccgggcgac
    11281 ggggtagtcg atgcaggtgt cgatggccag ccagccatca ccatcgcgca ggaggtagat
    11341 gttcacgtgg ttgagcacgt agggaatcgt gaagcggacc caatggagtc catccgcgat
    11401 ctcgacgggc gcaccgtctt cgggcttgtc gaaggggaac tgaagcatga tggttcagga
    11461 cttaactgat gttgcgcatc cggcgcaacc gccagtgttt tgacttgcac cctcgcggcc
    11521 cgcattgtct cgggtgccgg aaacttcggg aggctcaggt ggacaaggcg gatgtgatcg
    11581 tcgtcggcgg cgggcttgcc ggattggtcg ccgcggcgga actgggagac cgaggcaagt
    11641 cggtgatcct ggtcgaacag gagggcgaga attcccttgg cgggcaggcc ttctggtcgc
    11701 ttggagggtt gttcttcgtc aacagccccg aacagcggcg attcggcatc agggattccc
    11761 gcgagctcgc cctgcgcgac tggctgggct ctgcacagtt cgaccgaccg gaagacgaat
    11821 ggccgcgccg ctgggccgag gcctatgtgg atttcgctgc aggcgagaag cgctcctggc
    11881 tcgcggggca gggcgtcaag tggtttccca tgccgggctg ggcggaacgg ggcggcgggt
    11941 tcgcgtccgg tcacggcaat tcggtgccgc gctttcacct gacgtggggc acggggccag
    12001 gcatcgtcga gccgttcgag cgccgggtgc gagcgcatgt cgcggacgga cggatcgtgc
    12061 tgaagtgccg acatcaggtc agccgaattg ttatgacaaa tggccgcgcc gctggcgtcg
    12121 ccggcgagcg gctggaagaa agccgcgtcg ggcgtggcca gcagtcagga cgcggcatcg
    12181 tcggcgactt cgagttcagc gcggattcgg tgatcgtgtc gtccggcggc atcggcggca
    12241 atttcgatct tgtgcgcaag tcgtggccgg ttgaacgcct cggaccggcg ccgaagcaca
    12301 tggtggctgg cgtgccgcac catgtcgacg gacggatgat cggcatcacg cagcgggcgg
    12361 gcggcgcggt gatcaatgcc gaccgcatgt ggcattacac cgaaggcctg cacaactggg
    12421 atccgatctg gccaaaccac ggcatccgca tcattccggg gccatcgtcg atgtggttcg
    12481 atgcgcgcgg agaacggctg ccggcaccgt gtctgccggg ctttgattcg ctcggtacgc
    12541 tgaaggccat cctcgccaca ggtcacgagt attcatggtt cgtgctcacc aaggcgatcg
    12601 ccaagaagga gtacgtcctg tccggctcgg agcagaaccc ggacatgacc tcgaagagct
    12661 ggctgtccgt gctgaagagc cggcgcgggc gcggcgcgca tccgaacgtc gaggccttcc
    12721 tgaagcacgg caaggacttt gtcgtgcggg acgatctgcg cgatctggtc gctggcatga
    12781 atgcgttgac gggtacggac ctgatcgacc acgaccgcct caaggcccag atcgaggcgc
    12841 gcgacgccca gctcgaccat gccttttcca aggacttcca gatcatggcg atccagaatg
    12901 cacggcgcta cctcggcgac aagatcgtgc gcacggccaa gccgcaccgc ttcctggacc
    12961 cgaagaacgg cccgctgatc gccatccgcc tcaacatcct cacgcggaag acgctgggtg
    13021 gcatccacac cgatctggac gcccgggttc tggatggatc gggcgcggcg attgccggac
    13081 tttacgcggc cggtgaggcg tcgggcttcg gcggcggcgg ctatcacggc tacaacgcgc
    13141 tggaaggcac gttcctcggc ggctgcatct tttcgggccg cgtggcaggc cgggcggcgg
    13201 ggtgaccctc tcaggtcttg cggagcaagg aaactgcgac tttctggagc attttcaagc
    13261 gcagtggata ccggttcgcg tgaagaatgt gcgcaagaac agcagattga gcggctgaag
    13321 tgaccgtgcg ttcacttgaa acggtttagc aagcgggctt cgacgttgcg acagcgctgt
    13381 ggcggaaccg gcgctgcgcg ccgctacttc ttcttccgga acgcgtcgag gctgacgact
    13441 tcagcggtac ctggcgccgt gggcttttcg ctgtcgtccg ggtgtttctc ggcgggcggc
    13501 tcggactttg tctcggccgc aggggccttt gcgggcttct tgccgatgcg cgccggctca
    13561 ggtggagcct ccgcctcagg atcgggcttc ttcgctgcct tgcccttgcc ctttgcggcg
    13621 ggctttgttg ccacatcgtc gttctccggc gcgacctcgg ccgcctcgaa cttcaggccg
    13681 aatttgacgc tggggtcgaa aaagccggtc accgcctcga agggaacgac caggcgctcg
    13741 gcgatcttgt cgaaggacaa cgacacctcg aatccggtgt cgctcaccga caggtcccag
    13801 aactggtgct ggaggacgat agtcatctcg tcgggataca tgctcttgag gcgtgcggag
    13861 actttcgcgc ccggagcgtc ggtattgaac gagatgtaga agtgatgcgt gccgggaaga
    13921 ccttccttgg cggcatcggt cagcaccttc cgcaccacgc cgcgaagcgc ctgctgaacc
    13981 agaacgtcgt atcggatgag gtcggtggac atgaacggct tcgctcgcga cgtcagaatc
    14041 tatcggaaga ggctagcaag attcccgcgc aaggggaaat gcggcgacgc aaggccgggg
    14101 aaagtgggag gcttctgttg ccaggcgcct cccggccccg ccttcaggtg ctaccccgaa
    14161 ggactttggt tcggtatttc aaaccgcatt acgcggcctg agcaaccgga gcatagttgt
    14221 cattcgcaac tataggtttg acccgataac ggcggtatca tgccgggcaa aagtccaccc
    14281 tttacgccct cgtcgatcct gtttcgcccc cgccgcagcg catcccgttc aggcttgcgt
    14341 tccggtggag gcgccgggta ccgcccccgg gtccgatggg tttattacga tggccgttta
    14401 tcgccatagc cgtccgagga cggcacggtg aatataggcg ctcggacgtg gcattgcaaa
    14461 ggccacgatg cggcggaccg gcggcaaagg cgctctggac cgattttgtc gagaagtata
    14521 aggtcttagc cgtcgaaact cgattccgcc gggaggcctg acatggaatt gaaagaccat
    14581 atcgccgaca tcaagaagta cactgcctcg gtcgacgaga aggcggtcgc cggcatggcg
    14641 aaaacctatg cgctggtgat gagcaaggcg gattcccgct atgtcgccgt ctccgatccc
    14701 gacgaggtgg cgcgcgtcgt cgaaggcttc tgcaagaaga agctcggccg caaggaaaag
    14761 gacgccgatc tggtcgcggt cgtgaaagcc cagggcgaga agatgaaggg cgaccggacc
    14821 aagtcgcgca tcaccatcta ttacctcatc gccgagcact tcaagcagct cagcgctttc
    14881 catccgaagt agggcgatcc gtcctttcca cagggtgaag gacgcgcttt ccagcagaat
    14941 cggaatcggg ataggctttg acgatgaaag cctatcttga tctgatgcgt ctggttctgg
    15001 aaaccggcgt tgacaaggcg gaccggaccg gcacggggac gcgctccctg ttcggcgcgc
    15061 agatgcgttt caacctggcg gacgggtttc cgctggtgac aaccaaacgc ctgcatctga
    15121 agtcgatcat tcacgaactc atctggttcc tgaaggggga caccaacgtc gagtacctgc
    15181 aggagcgcgg cgtcagcatc tgggacgaat gggccgacga gaacggcgac cttggcccgg
    15241 tctacggcaa acagtggcgc aactgggatt cccccgacgg gcgacacatc gatcagatcc
    15301 gcgaggtgat cgcccggatt cgcaaggacc cggattcgcg ccggctgatc gtcacggcct
    15361 ggaatccggc cgatatcccg ttcatggccc tgtcgccgtg ccactgcctg ttccagttct
    15421 acgtggccga ggggcggctg tcctgccagc tctatcagcg gtcggcggac ctgttcctgg
    15481 gcgttccctt caacatcgcc tcctatgcgc tgctgacgca catggtggcc caggtcaccg
    15541 gcctgaaggt cggcgatttc gtgcacacct tcggcgatgt gcatctctat gcgaaccatc
    15601 tggagcaggc ccgtctccag ctgacgcgcg aaccgaagtc gttgccacgt ctctggctcg
    15661 acccctccgt gaccaatatc gacgatttcc gcttcgagca catccgcatc gacaactacc
    15721 tcttccatcc cgccatcaag gcgccggtgg cggtgtgagt tcggccgccg ttcatcggca
    15781 ggcgcccatg gctgccgcca ttgatggcga aaggggcgat catgcgagtt gcggcgattg
    15841 cggttctggc gctggtgctg ccggcgctgg ggggatgcaa gatggcgctg ctcaagggcg
    15901 cgacgcggct ggcgctcggc gcggagcagg cggaaacact ggatccgctg gcgatggtgc
    15961 gtcgccagtt gcggtcccca ccgccgcgcg cgccttcgcc agccgacgcg tcttcgcgcc
    16021 cgcccgatcc tgccaggtga cggcatgacg acgctcgtga tggtcgcggc tgtcgcacgc
    16081 aacggagcca tagggcgcga caaccggttg ctctggcgcc tgaagagcga cttgcagcgt
    16141 ttcaagcgca tcacgctagg caagcccatg atcatggggc gcaagacgta tgactcgatc
    16201 ggcaggccgt tgccgggacg cacgacgatc gtcgtcacgc gcgaccccgc cttcgccgcg
    16261 gcaggcgtga tggtcgccgc ttcgccggag gcggcgttgc atctggcgcg gcaggaggcg
    16321 gaaaaactcg gtgttcccga cgtcatcatc gcgggagggg gagaaatcta cacccgcttc
    16381 ctgccgattg cggatcggct cgagatcacc gaggtcgacc tggcgcccgt tgcggatgcc
    16441 tactttccac cgctcgaccc gacgctctgg aaggtcgcgg cacgagaggc gcatgcgccg
    16501 gggccaggcg acgagacggc tttcaccttc gtgacctacg tgcgggcgga tgcctgacac
    16561 gaagcttgcg ctttgccgat ccttgacctt ttgccgaaag tcaccacttc cccacgatgg
    16621 agcgcattgc ggttctcccg ccgcttgcct atatcgacgc aacgagtttt ccccggaaac
    16681 gaggactata tgccctggag caacaatacc ggcggcggcg gcccctggca gcccaagaac
    16741 aacggcccct ggggcggtgg cccgcaaggt ggcggtggtg gtggcggcgg gggcggcaac
    16801 cgtcccccgg atcttgagga tattttccgc aagggacagg accgtctgaa gagcattgtg
    16861 ccaggcggcg gcggcgttgg cggcattggc gtcgcgctga ttgcggtcgc cggcgtgctc
    16921 gtctggctcg cgaccggtat ttacaccgtg cgcccgaacg aaatcggcat cgagcgcgtg
    16981 ttcggccagt tccgcaatgt cgcgccgccc ggtctgcact acaactggcc agcgcccatc
    17041 gggcgggtgg acaagccggt gattgccgtg cagtccgtcg aaatcggcat gcgcaccgaa
    17101 atggggcgcg gcgggcttcg gcgcgacgtg ccgcaggaaa gcctgatgct gacgggcgac
    17161 gagaatatcg tcgatatcga tttcacggtt ctgtggcgcg tcgatgagcg caagccgcag
    17221 gatttcatct tcaacatgcg caacacggaa gcgacggtga aggctgtcgc ggaagctgcc
    17281 atgcgcgagg tgatcggcaa gtccaacgtc atcccggttc tgaccggcgc gcgccagacg
    17341 acggagagcg ccgttcagga gttgatgcag aaagtcctga acgaatacgg cgccggcgtc
    17401 acggtccagc aggttcagtt gcagaaggtc gacccgcctg cgcaggtcat cgatgccttc
    17461 cgcgacgtgc aggcggcccg cgccgatcag gagcgcgcgc agaacgaagc gcaggcctat
    17521 gccaacaagg tcgtgccgga agcgcgcggc gaggcggccc gcatcatcca gcgggccgaa
    17581 gcggagaagg aaaagatgat tgccggcgca cgcggcgagg cagcccagtt cgtcaagctc
    17641 tacgacgaat acaagaaggc gccggaagtg acgcgccagc gcctgttcct cgaaacgatc
    17701 gaacgcattt atggcggcat ggacaaggtg atcctggatc agtccggggc cgggcagggc
    17761 gtcgtcccct atctgccgct tgatcaactc aatcgccgtt ccggcacgca gggagcgcca
    17821 cgctaatggc actgcacccc atcatcgccg gcgtggtcgg cgtcgccgcg gctgcggcct
    17881 ttacgtcggt gttcgtcgtc gatcagacgc aacaggccat tgtcacgcag ttcggcaagc
    17941 cgcagcgcgt ggtcgtgaag ccggggctca acttcaaggc gccgttcttc ttcgagtccg
    18001 tcatccatct ggacaagcgc atcctggatc tcgaatcggc gtcggaggaa gtcatcacgt
    18061 cggaccagaa gcgtctcgtc gtcgatgcct tcagccgcta tcggatcgag aatccgctgc
    18121 agttcttcca ggccacgtcc ggctccatcg cggcgggcga ggaccggttg cgcacgctgc
    18181 tcaattcgtc cctacgccgc gttctgggcg aatcgagctt tgcggccgtg gtgcgcgacg
    18241 agcgcgccgg cctggtgctc aaggtcaagg aacaactgaa ccgcgaggcc caggccctcg
    18301 gtatcggcgt tgtcgatgtg cgcatccgtc gcgccgatct tccggagcag aacagccagg
    18361 cggtcttccg gcgcatgcag acggagcgtc agcgcgaggc gtcggagtat cgcgcgcaag
    18421 gcgtcgaggc gtcggaacgg atcaaggcgc gcgccgaccg cgacgtcgtc gtcacccggg
    18481 cggaagccga acgccaggca cgcgagacgc tgggctcggg tgatgcggag cgcaccaagc
    18541 tgctggccga tgcctacggg cgggatggcg agttcttcgc gttctggcgg tcgatgcagg
    18601 cctatgaggc cggattgcgc gccggcgaca cgaagatgat cctgacgccg gacagcgact
    18661 tcttccgcta tctgaacgat cccaaggggc gcgcgcgcgc gcccaatggc gagaaaaagc
    18721 aatagccggc ggcggcggag gcgaccattc gggatattgt cttcgccgtc gcactggtgc
    18781 tggccttcga gggaatcgtc tgcgccgcat tcccatcggc catgaagcgg gccatgcagc
    18841 aggccggtca cctgccggaa ggcgcgatgc gcatcgccgg gcttgcgtcg gcggttgtcg
    18901 gggtcgcgct gctgtggttg atcaaggccg cgtgaaaagt gacacaattt tctatatgac
    18961 tatggctttg acgcgttttg gccgcaattg ataccttcat cacagcccgt cacggggcga
    19021 ccatgacaga atccagaggg acacagatgt cgactcttcc tatgcgtagc gttcgcggca
    19081 cactggccaa cagggccagc cgagcgacgc gggccgtcgc gaccatcgcg ctcggtgcca
    19141 gcctgtttct gacgcaggtg ccggcggcat tggccagagg gccggaatcg ctggccgatc
    19201 tggccgccag cgtcatggac gcggtggtga acatcaaggc gtcacagacg gtgcaacagc
    19261 gctcggttcc catgccgcag ttgccgccgg gatcgccttt cgaagacctt ttcaacgact
    19321 tcttcaatcg gcgcgggccg ggcggcgagg gccagcaggg ccgtccgcag cagcgctcgt
    19381 cctccttggg gtccggcttc gtgatcgatg cctccggcat cgtggtgacc aacaaccacg
    19441 tgatcgccga cgcaaacgag gtgacggtgg tgttcaacga cggcaccgaa ctgaaggccg
    19501 aggtggtcgg caaggactcg aaggttgatc tcgcggtcct gcgcgtgaag accgacaagc
    19561 cgctgaaagc cgtcaagtgg ggcgattccc aaaaggcacg ggtcggcgat tgggtgatgg
    19621 cgatcggcaa tccgtttggc ttcggcggct cggtttcggc tggcatcgtc tcggccaagg
    19681 atcgcaatat ccgctccggt ccctatgaca acttcatcca gaccgacgcg gcgatcaaca
    19741 aggggaactc cggcggcccg ctgttcaata tggccggcga cgtaatcggc gtgaacaccg
    19801 ccatcatctc gccgaccggc ggatcgatcg gcattggttt cgccgtgccg tccgccatgg
    19861 ccatgggcat cgtggaccag ctcacgaagt tcggcgagac ccggcgcggc tggctcggcg
    19921 ttgccattca ggacgtggat gacgcgatgg ccgagagcct cggcatcggc aaggcgcgcg
    19981 gcgccttcgt ctccagcgtg gatgacaagg gcccagcgaa gccggcggga atcgagcgtg
    20041 aggatgtcat catcaagttc gacggcaagg atgtgcgcat gtcgcgcgat ctgccgcgca
    20101 tcgtcgccga gacgccggtg gacaagcagg ttgatgtcgt catcctgcgt ggcggcaagg
    20161 aagtcatccg ccaggtcaag atcggccgcc tggaggacaa tgaccgtcgc gccagcgccg
    20221 cgccgggcaa tgcgccgtct tccggcaaca acagcaatgc ctcgcgcacg gtgatcggtc
    20281 tcgatctgtc tcctctcaat cccgatctgc gccgccggtt caacatcaag gacagcgtca
    20341 atggcgtggt catcacacgc gtggcgccgg gatcgcgcgc tgccgagcgc atgatccagc
    20401 cgggcgatgt gatcgtcgag gtgcagcgca cgccggtcaa gaccgttgcg gaggtatcga
    20461 gccgcctgga tgaactgaag aaggagaagc gcgccaatgc gctcttctac gtggcgaacg
    20521 cgaacggcga cctgcgctac atcacgctgc cggtggagtg atcctgtccg tcgcctggta
    20581 caggccggca ttcagcagac agtagaaagg gcggtcgaaa ggccgccctt tcgctgtgtc
    20641 ctccaggatc gaaaggcacc cgaaacgacc atgctctaca gggcgaattc gtccctgcgg
    20701 tatccctgcg cgtagaggag ggcggtcagg tcggcatgtt cgatccgggc atgggcttcg
    20761 gccgccacgg cgggtttggc cctgaaggcg acgcccagac cggcttcgcg gatcatgtcc
    20821 agatcgttgg cgccgtcgcc gactgccagg gtctcatggg cggccaatcc caaccgctcg
    20881 cgcacctcga tcagggtcaa ccgcttggcc tccttgccga ggatcggttc cgccacgcgg
    20941 cccgcgagca ggccctggtc cagaacgagt tcgttggacc ggtcggcggt gaagccgatc
    21001 gaggcgccga tcttgcgggt gaacagggtg aacccgcccg aaacgagcca ggcctcgccg
    21061 ccatcgcgac gcatggtcct taccagttcg gcgccgcccg gcgtgagcgt tatgcgttcg
    21121 gcgatgatcc tgtccgcgat ggtcgcgggc aggcccttca gcagcgccac gcgctcccga
    21181 agggcgggtt cgaaggcgat ctcgccgcgc atggcccgtt cggtaatctg cgaaaccttg
    21241 tccttgaggc caacgaaatc ggcgagttcg tcgatgcact cctggccgat catggtggaa
    21301 tccatgtccg ccaccagcag tcgcttgcgc cgtgtggccg ccggctgaac cacgacgtcc
    21361 gccggtaggc ccgcgagcat cagctggacg gcgagcgccg cgccgcgaca gtcgaagtgg
    21421 ttgtcggctt cgaaggtcag gtcggcggcg atgcctggcg ccagccattt cgccgcatcg
    21481 gcatcaggta ggccttgcgc tgcggcgcgc aagtgcgctt cctccagcac cggagtcgat
    21541 ggattggcta tgagtgtcgc gacgaacagc atggaaccgg cggaagccct ttcggagata
    21601 gcccggcgct gccgcgccgg cgcgcttgac gccgtgctga tcgcaggtcc tacggccagc
    21661 ggcaagtccg ctcttgcgct ggcgctggcg gaaacgcttg gtggcacggt ggtcaatgcg
    21721 gattccatgc aggtctatgc ggatctccgc gtgctctccg cgcggccgac gcgggatgag
    21781 gaagcgcgcg ttccccaccg tctgtatggg atcgtgccgg ccgcgcgcgc ctattcggtg
    21841 ggcgactacc tgcgcgatgc ggcaccggta ctggccgatc tcaagcgggc tgggcgggtc
    21901 gcggttatcg tcggcggcac ggggctgtac ttccgggcgc tgaccacggg gctggccgaa
    21961 acgcccgtgg tgcctcaaga ggtgcgcgag cgggtcgagg ggttggcgcg gcacggcgcg
    22021 gacctgcatg cggaactggc caggcgcgat ccggaagccg cagcccgcct cagcccggcc
    22081 gacagcccga ggatccagcg cgcgctggaa gtcttcgagg ctaccggaac gcccatctcc
    22141 cggtggtggc agtcgcatca gtccgttccg ctgctcgccc ccgggcgctg gcagggactg
    22201 ttcctcaacc cttcgcgcga tgccttgttt gccagaatcg atgcgcggtt tcgcgagatg
    22261 atgggtcagg gcgcgctgga ggaggtgcga cacctggcaa cgcttggcct tgcggccaat
    22321 cgtggggtga tgaaggccca tggcctgccg catctcgtgg cccatctcgc cggcgcgctg
    22381 gacctggatg aggccatccg gctgggacag cgcgacacgc gccgctatgc caagcgtcag
    22441 ctgacctggg cgcgcaagtt catgccggac tggatctggg cggagtccgt cccggcgctc
    22501 ggcgggttgg tgccgccggc ctgaaccgtc tcaggcggcc ttgcggccct cttcggtgtg
    22561 cgtgcggttg cgaccgtcgt tcttggcgct gtacagcgcc cggtcggcgg cgacgaggaa
    22621 ctcctcatac cccagccctt cccggctttc cgcaacaccg caactgatgg tgaccgtgaa
    22681 cgcgttcgcg ccatccgaaa tcgtcatctg ctcgattcgt tgacgcagac gctcggcgat
    22741 ggcgatggct gttgtcgcat tggcgtcgta caggagcatc gcgaattcct cgccgcctgt
    22801 tcgggccaag atgtcccttt gactgcggca acaggactgg gctgctgtcg cgacggcctg
    22861 gatggccttg tcgccggcac tgtggccgta ggtgtcgttg atccgcttga agtagtcgat
    22921 gtcgatgacg atcatggcgc agctgacctc gttcctggcg gccagcttca aggttgcgat
    22981 ggattgttcc ttcatcgacc gccggttggc gacgttggtc aacggatcga gatgggcgag
    23041 gttccacagt tcctgctcca ccagggtgcg gcgctccatc tcctcggtca gccgttttct
    23101 ctgctcctcc agcatcatgt tgagctgctt gagctccgtg atgtccgtga agctgatcac
    23161 cagcccgtcg ttcatcgggg cggcgacgat gcgataccag cgcgccgtgc caccccgttc
    23221 caggacggcc tcgtaggaca agcactggcg cgtttgcagc acctcgacat gctgttccca
    23281 cagaccttcc ctgcgcaact cggggaacag gcggctgacg ggagtggcca gaacgtcctg
    23341 ctgctcgcgc ctgagcattt ccagcatggg cgcgttggct gccaggacgg ctccgtcgat
    23401 cacgttccca tgcccatcac gcgtcgcgcg caccgccagg atgccgtcgg cggccgcatc
    23461 gaggatttcg cgcagcagtt catcgctgta ttcgcgggga cggttgtagg agcagatgac
    23521 aatgccgccg cgcccgtcat cgagcggaag atgcaggcgg ccccagaggt gaacccgtcc
    23581 gttttccgcc tccctgtcga tggcgaacag cggcgcgcgc agctccaacg tcttgaggaa
    23641 ggtccggcgc gcgcttgcac cggactgcgg cgactgatgg gccgtggact ttccggtcag
    23701 gtcggatcct gtcaggcgtt gcagcgcctc gccgaagtag agatactcga actctccgtg
    23761 ggcgttcttc tcgaccagcg tcaggtcgcg tccgatcgtg cgcatggtca cgctgtcgat
    23821 caggcggaca tgcggcaggc cgccggcgcg ctgggcctgc cgatagcggg caagcagttc
    23881 gcggctctcc ggcgtgcgca gatgatcctc gatgtccgag acgtacacct gaaagcgacc
    23941 cgttgtgaaa atacacgtat tagatgtata tcgcacatgt tatttccaaa tggttgcatt
    24001 gctgtcaggc cggcggcttc gcacggcgcg gacttcgcca agcgtgcggg ccggcctgcg
    24061 aggcgaaaaa cctgatttgt cagttgacgg cgccgcaaac gcttcctagt gtccgcgccg
    24121 acggagatgg ccgatggctt tcatcattct tgtatgcaat gcgccggagg tgggacgggt
    24181 ctaggcccga tagtcccgac gccggcgcat atccgaggcc cctcaggggc ctttttcttt
    24241 gtccgaaacc gatgatgtga acgagcaggc aggagaacga gatgagcgag acgatgaccg
    24301 gagcggaaat ggtggtccgc gccctgcagg atcagggcgt cgagcacctg ttcggctatc
    24361 ccggcggcgc ggtgctgccc atctacgacg cgctgttcca gcaggacaag gtgcagcaca
    24421 ttctggtgcg gcacgagcag ggcgcggccc atgcggcgga aggctacgcc cgctcgaccg
    24481 gaaaggttgg ctgcgtgctg gtgacgtccg gtcccggcgc gaccaatgcg gttaccgggc
    24541 tgaccgatgc cttgatggac tcgatcccgc tgatctgcat cacggggcag gtgccgacgc
    24601 atctgatcgg ttccgatgcg tttcaggaag cggacacggt cggcatcaca cgccactgca
    24661 ccaagcacaa ctatctggtg aagcgggtcg aggacctgcc gcgcattctg cacgaggcct
    24721 tctacgtggc ggcgaacggg cgccccggcc cggtggtcat cgacattccc aaggacgtgc
    24781 aattcgcccg gggcaagtat tcccgcccga ccaattccga gcacaagacc tatcgccccc
    24841 gggtggacgg cgacatggcg ctgatcgagc gcgcggtgga cctcatggcg aaggccaagc
    24901 ggccgatctt ctacaccggc ggcggcgtca tcaattccgg tccggcggca tcggccgcgc
    24961 tgcgcgatct ggtgcgcgcc acgggtttcc ccatcacctc gacgctgatg gggctcggcg
    25021 cctacccggc ggccgatccg caatggcttg gcatgctggg catgcacggc acctacgaag
    25081 ccaatcttgc gatgcatgac tgcgacgtga tgatcaacat cggcgcgcgt ttcgatgacc
    25141 ggatcaccgg ccgcatcgat gcgttctcgc cgaagtccaa gaaaattcac gtcgatatcg
    25201 atccgtcctc gatcaacaag aacgtgaagg tcgatatcgg catcatcggc gattgcgggc
    25261 gcgtcctcgt cgagatggtg cgcatctgga aggagaagaa gcacaaggcc gacgccgaga
    25321 tgctgaaggc ctggtggggg cagatctcca agtggcgcgc gcgcaattgt ctcgcctaca
    25381 agcgctccga gacggagatc aagccgcagt atgccgtcga gcgcctctat gcgctgacca
    25441 agggcaggga tgcctacttc accaccgaag tggggcaaca ccagatgtgg gcggcgcagt
    25501 tcctgcattt cgaggagccg aaccgctggc tcacctcggg cggtctcggc accatgggct
    25561 acggcctgcc ggcggcagtg ggcgcgcagc tcgcccatcc gaagtcgctg gtcgtctgcg
    25621 tcgcggggga agcctcggtg ctgatgaaca tgcaggaaat gtccaccgcg gcgcagtatc
    25681 ggctgccggt caagatcttc atcctgaaca acgagtacat gggcatggtg cggcagtggc
    25741 aggaactgct gcacggcgga cgctattccg agagctattc ggcggcgctg ccggatttcg
    25801 tgaagctcgc cgaggcctat cacgccaagg gcatccgctg ttccgatccg aaggaactgg
    25861 acgcgaaaat ccaggagatg atcgattatc ccgggccggt gatcttcgac tgccttgtca
    25921 ccaaggaaga gaactgcttc ccgatgatcc cctcgggcaa ggcgcacaat gagatgatcc
    25981 tgccggattt cgagggcgac accggccaga tcatcgatgc caagggcaag cagctggtgt
    26041 gacgatgagg cccaccctcg ccgatctgga gcaggcgcac gccgtcgttc gcgccgccat
    26101 gccgccgacg ccgcagtatg cctggccgtt gctcggacag cggctgggcg ccgaggtcgt
    26161 ggtcaagcac gagaaccaca ccccgaccgg ggcgttcaag gtgcgcggcg gactggtcta
    26221 catggaccgg ctcaagcgcg agcgcccgca ggtcgccggc gtcgtttcgg cgactcgcgg
    26281 caatcacggc cagagcctcg ccttcgccgg tggccggcac ggggtggcgg tgacgattgt
    26341 tgtcccgcgc ggcaattcga tcgagaagaa cgcggcgatg cgcgccttca gggccgacct
    26401 gatcgagcac ggcagcgact tcgaggaagc gcggcaagag gcgcggcgcc tggcggagaa
    26461 ccggggcctc gaaatggtcc cgtcctttca tccggacctg atgtgcgggg ttgcgaccta
    26521 tgcactggaa ctgctcaccg cgcatcccga tctcgacgtc ctctatgtac ccatcgggca
    26581 gggctcgggc atctgcgggt gcattgccgc gcgggacgct ctgggtctga ggacggagat
    26641 cgtcggcgtt cagtcgaccg aggcgccggc ctatgcgctg tccatggccg cgggccacgc
    26701 ggtttccacc aacagcgcca acaccaaggc cgacggcatg gcgacgcgcg taccggatgg
    26761 cgaagcgctg gcgatcatcc tgaggggcgc agcgcggatc gtcacggtca ccgatgatga
    26821 gatcgctgtt gccacgcggg cctactggac cgacacgcac aacatcgccg aaggggcggg
    26881 cgccgccgcc cttgccgccg cgatgcagga aacgacgcgg cttgccggcc ggcgcgtcgg
    26941 gctgatcctg tcgggcggca acatcgattt cgacctgttc cggacctgga tctgcggcgt
    27001 gcccgcgtcc caacccattt gagcgagcga gagccatgaa atcccactat tccgacccat
    27061 cccggcacca gagcgtggaa cggcgcaccc tggcgatcat cgtcgacaac gagccgggcg
    27121 tgcttgcccg catcgccggc atgttctccg gccgcggcta caatatcgaa agcctcacgg
    27181 tttccgagac cgagcacgag aagcacctct cgcgcatcac ggtcgtgacg cggggcaccc
    27241 cggaggtgat cgaccagatc aaggcgcagc tcgaccgcct cgttcccgtg caccgggtcg
    27301 tcgacctgac cctctccggc agcgcgatcg agcgcgaact ggcgctggtc aaggtggcgc
    27361 aggagggcgc gacgcgcagc gagcatcgca tggaggcgct gcgccttgcc accgcgttca
    27421 acgcccgcac cgtcgacgcc acgctcggct ccttcatctt tgagatcgcc ggaacgagcg
    27481 acgacgtcga gcgctttgtc tcgctgctgg ggcagatcgg cctcgtcgag gtggcgcgca
    27541 ccggcatcgc ggccatcacg cggggtgccg ggggtctcta gatgtccgcg tcggcgacac
    27601 tcgcggcgct cctcgtcttc tccttcgtga actcggtcac gccggggccg aacaacctga
    27661 tgctgctcgc ttcaggggtg aacttcggct tccgccgcac cattccgcat atgctcggca
    27721 tcggctgcgg tttcatggtg atgacggcgc tggtgggcgt aggcctcagc caggtcttcg
    27781 agcgctttcc cattgcctat ctggcgctga aggtcgccgg ggcgggctac atggtgtggc
    27841 tcgcctggaa gatcgccaca tcgggtccgg tcggcgaagg gacgtctcgc ggcaagccga
    27901 tgaccttcct gcaggcagcg gccttccagt gggtcaatcc caaggcctgg atgatggtcg
    27961 tgacggcggt ttcgacctat tccatcaccg acagcgtggt tgtgaacgcc ttgaccattg
    28021 cccttgtctt cgggatcgtg aatccgccca gcatcagcgt ctgggtcctg ttcggcaccg
    28081 ccatgcagcg ctggctgcgt gatccagttc gtctgcgcct gttcaacggg tgcatggcgg
    28141 cggcgctgat tgtctcgctc atcccgatgc tgcttgatgg caaaccttag ctgctgctgc
    28201 tcttcccgct tgaccgggcc gaaacttagg cctttaagcg ccgtcgaaat tcccttccgt
    28261 ttatgacatc tgacaggagt tcccatccca tgcgcgttta ctacgaccgc gacgccgatc
    28321 tgaatctgat caagggcaag aaggtctgca tcgtcggcta tggttcgcag ggccgcgccc
    28381 atgcgctgaa cctcaaggag tccggcgtca agaacatcgt catcgcgctg aagccgacct
    28441 cggcaaccgc cgagaaggtg aaggccgacg ggctgaaggt catgtccgtg gccgaagccg
    28501 ccaaatgggc cgacgtcatg atgatggcga cgccggacga gttgcaggcc gacatctaca
    28561 acaccgagat cgcccccaac atccgcgacg gcgcggcgat cgccttcgcg cacggcctca
    28621 acgtgcactt caacctgatc gagccgaaga agtcgctcga cgtgatcatg atcgcgccga
    28681 aaggcccggg ccacaccgtg cgcggcgagt accagaaggg cggcggcgtg ccctgcctga
    28741 tcgccatcca ccaggatgct tccggcaacg cgcatgacat cgccctgtcc tatgcttgcg
    28801 gcgtgggcgg cggccgttcg ggcgtgatcg agaccaactt caaggaagag tgcgaaaccg
    28861 atctgttcgg cgagcaagtc gtgctctgcg gcggtctggt cgagctgatc cgcgccggct
    28921 acgagaccct ggtcgaggcc ggctacgccc cggagatggc ctacttcgag tgcctccacg
    28981 aggtgaagct gatcgtcgac ctcatctacg agggcggcat cgccaacatg aactactcga
    29041 tctccaatac ggcagagttc ggcgagtacg tgaccggccc gcgcatcatc accgacgaga
    29101 cgcgcgccga aatgcgccgc gttctgaagg acatccagac cggcgccttc acctcggagt
    29161 ggatccgcga gtgccgcgcc ggccagccga agttcaaggc cacgcgccgc atgaacgatg
    29221 cgcatccgat cgaggaggtt ggttccaagc tccgcgcgat gatgccgtgg atcgccaaga
    29281 acaagctcgt cgacaaggcg cgcaactgat cccgcgtcgc cggtcggtca ccggcacctg
    29341 tcggatacgt gaaaggcccc gccgtcgcgc ggggcttttt tcgttggcgc gatcgcgtat
    29401 ctgataggcc gcaacggaga gccccggcat gacgcgtttc tatgacgacc tgccgcgcga
    29461 agcggatttc ggcgccctgc tcgaaccggc cgcctatgtg ccgctgccgc atgactggct
    29521 ggttggcgtg accgacgtgg tcgattccac cggcgccatc gccgccgggc gctacaagtc
    29581 cgtgaacatg gccggcgcga cggtgatcgc cgcgctgacg aatgccttcc ccggccggag
    29641 ctttccgttc gttttcggcg gcgatggcgc gagcttcgcc attcccggga cacaggagag
    29701 cgcggcgcgc gccgttctcg ggcaaaccat ccgcttcgtc gaggaagcgc tcggtcttgc
    29761 cctgcgcgcg gccctgatcc cggtttcgga tctgcgtgcg gcgggaaagg acgttgccgt
    29821 ggcgcggcac gccgcgtctg catcgatcgc ctacgccatg ttccggggcg ggggcctctc
    29881 ccatgcggaa gccgagatga aacgcggcgc ctatgccgtt gcgccatccg cgccgggtga
    29941 tgcgcccgat ctgaccggcc tgtcctgccg gtggagcccg atcccggcgc gtcacggcgt
    30001 gatcgtctcg ctgatcctgt cgccggccca cggcgaggat gcggagtttc gtgcggtggt
    30061 cggcaaggtt ctgggcttgg cctcggaagc cgggcaggcc gcgcgtccgg tggcgttggc
    30121 ggaggatatt agcgtgcgtt ggcctccgga cggcgccgtg aacgaggccc tgacccgtcc
    30181 cgtctggcgg cgggcgccgg ccctggcgcg gctctacctg cgcggcctga tcgcctttca
    30241 gaaactcatc ttcgcccgca actggaccgt tggcgccttc gatccggccc gctaccgtcg
    30301 cgatctcgtg gccaacgccg atttccggaa atatgacgac gggctgaagc tgacgctcga
    30361 ctgtacgccc gcgctggccg accgcctcga ggcgctgctg gccgaggccg agcgcagcgg
    30421 cgtggcgcgt ttcggattgc atcgccaggg cgcggcgatc gtcacctgct tcgtcccctc
    30481 cgcggcgcgc aatgatcatg tgcacttcat tgacggtgcc gacggcggct acgcgcgggc
    30541 cgcgcacagg ctcaaggcga agcaggcggc gtgactggcg cctagggaat ggccagttcc
    30601 accaccaccg gacagtgatc ggacgccttg ggtcggtccc agccgatgcg cggaaaccgc
    30661 ggaccttcat agcggcgcgc acgatacggc tgtcccatgc ggataatgtc cgggacggca
    30721 tcgggatttg ccttggcgag cgccggcgag accagcagca tgtcgagttg cgcatactgg
    30781 tcttccgggc cgtgataggt ggtccaccgc tcgtccggtt cgcgccggcg gacgatgttc
    30841 tcgctgaacc cgtcggcgaa caatggttcc aggccgtgcg gcgccacctt gccggtgtcg
    30901 tccagcagcg gcaggccatc cacctcggtg aagtcgttca tgtcgccgca gatgatccag
    30961 ttggcgtccc gcgcatgccg accgaagcgg tcctcgatca gccggcggat gccgagcgcc
    31021 tcggccttgc gcatgggcct ggtcagttcg cgcgaggggt tcatcgactt gaggtgacag
    31081 acgtagagcg tcagcggccg actgccgacc tcaaggtcga tctccaggca atcccggcgg
    31141 aacagccgat cgttctgcgt caggcgccgc tcgcgaccgt tgatcctttc acggtagttc
    31201 ttcaggtcct cgttccagac gccgagctcc tggaaggtcc ggtgcgcgtg cgtgcgcacc
    31261 cggatcggga ccttggacat gacgccgaca tcgatgccgc gcccgtcgtt gccgtcgagg
    31321 acaacgcgcc agtcgtagta gtgccggcga tcgacggcga acaggtccga atcctccggc
    31381 cggcggccag cgcggcgggc ttcgcggcgc agttcgttct tctccgcggc cgcctcaggc
    31441 tcgatcatcc gcttcacgta gcgctggtag aaaacctcaa gcgattcctt gctgtccacc
    31501 tcctgcaagg cgacgatgtc cgcccggcag gcatgcaggg caagcgcggt gagctggcgc
    31561 ttgtcatcgg atgcggcagc ctcgacaatg cgacgggcga gtttcagttc ctccggattg
    31621 tcgaactggt aggcgccgac gagattgtcc tcgggtggca tggcatcggg aaatgccagg
    31681 cgcgtgaaca ggttctcgac gttgaaggtg gcgacgcgga tcgtgttgcg gcggctcatg
    31741 cgcatgttca tcccggtgga ggatcgccgc aggataggcg agacgaccgg cgatcccaag
    31801 cgcgaatgca gccggtgctt gcggtcggcc tgccggttct ctacttcttg acgctgccgg
    31861 cccagaagag ccgggcaggg gagaaggcgt ggcgtcgaac cagaacaaca cggcagagcc
    31921 ggcgatccgg ttcgatggcg tttccatttc ctacgtcctt gcggatggcg gacggtacga
    31981 cgccgtcgcg ccggtggaca tcagcatcgc cgacggggcg ttcgtttcca tcgtcggtcc
    32041 gaccggatgc ggcaagtcga cgctgctcaa tgtcgcggcc ggcctggccg cgccgaccac
    32101 cggctccgcc acgatctttg gcaagccgct cgccggcatc aacgcgcagg ccggctacct
    32161 gttccagcag gatgcgctga tgccgtggaa gacggcgcag gacaatgtcg cgctcggtct
    32221 ggaagtgcag gggctgccgc gtgcgcaagc tctggcgaag gcgcatgaca tgctgcggcg
    32281 cgtcggcctc aagggtttcg gcgagcgcta tccgcatcag ctctccggcg gccagaagaa
    32341 gcgtgtggcg ctggcgcagg tgctggtgcg cggccccaag atcctgctga tggacgagcc
    32401 gttcggaccg ctggatgcgc agacgcggct tatcatgggc gaactgctgt cgggcctctg
    32461 gcaggccgac cgcaaggcgg tgatcttcgt cacgcacgat ctcgacgagg ccattggcct
    32521 ttccgaccgc gtgctgatca tggccgccgg gccgcagtcg cgcatcatcg gcgatttcga
    32581 gatcgggctg gagcggccgc gcgatcttgc cgagatccgc tccgacagcc gcttccacac
    32641 gctgcatgcg gagatctggg ccgtgctgcg cgacgaggtg cgcaagacct atcgcgacgt
    32701 ggaggcggca tgatgagccg cgccaagctc ctcgtccttc aggtcggcgt cgcggtcatc
    32761 gccatcgcca tctggcatgt ggtgaccacg acgaacctca tcggcgacca gaaggtgatg
    32821 aaattcttct tctcgacgcc ggccgatgtg ttctggcgga cctggaaggt gtttgcggaa
    32881 ggcacgatct ggcgccacct cggcattacc ctgtgggaga cgatcctcgc cttcgtgatc
    32941 ggatcggccg ccggcatcgt catcggcttc tggttcgccc gccagccgct ggtcgcggcg
    33001 gtgttcgacc cctacgtgaa gatgatcaac gccctgccgc gcgtggtgct ggcgcccatc
    33061 ttcgcgctgt ggttcggtct cggcatctgg tcgaaggtgg cgcttggctt cacgctggtg
    33121 ttcttcgtgg tgttcttcaa tgtctaccag ggggtcaagg aagtgaaccc cgtggtgctc
    33181 gccaacgccc ggatgctggg catgaacgaa cgccagttgc tgcgccatgt ctactggccg
    33241 gcggcgctgt cctgggtgtt ttcctcgctg cacacttcgg tggggttcgc gctggtgggc
    33301 gcggtggtcg gcgagtatct cggctcctcg gcggggctcg gctatctcat ccatcaggcg
    33361 gaaggcgtgt tcgatgtcgc cggtgtcttt tccgggatgt tgatcctcgc cgttttcgtc
    33421 gtcatcatcg atggcctggt gacgatgatc gaagggcggc tcctgaaatg gcggccctct
    33481 acggaaatca agacgcaggc atgatgcctg cccaactctt ctggaggaaa cgatgcggat
    33541 ttcgagacgt ggacttctgg tggccggcgc cggcctggtg gcgatgccgg gcatcgtccg
    33601 ggcgcagagc gtggagaagc cgaagctggt gctcggcgtc ggcggcaaga acctgctcta
    33661 ctatctgccg ctgaccatcg cggagcggag gggcttcttc aaggagcagg gccttgacgt
    33721 cgagatcacc gacttcggcg gcggcgcgaa ggcgctgcag tcgctggtgg gcggttccgc
    33781 cgacatcgtg acgggcgcct acgagcatac gatccgcatg caggcgaaag gcatcgatat
    33841 ccgctcgacg ctggaactcg gccgtttccc tgccatcgtc gtggcggtca agaaggacct
    33901 tgccggcaag gtcaagtccg ccgctgacct gaagggcctg aagatcggcg tcacggcgcc
    33961 gggctcctcg accaacatgc tcgtgcagtt cgccatggtg aaggcgggcc tgaagccgat
    34021 ggacgcggcc tgggtcggtg tcggtgcggg cgcttcggcc gtcgcggcca tgaagaaggg
    34081 cgagatcgac gcgatttcca acctcgatcc ggtgatttcc aagcttgagg ccgacggcga
    34141 catcgtggtg ctgatcgaca cccgcaccga ggccggtacg caggcgctgt tcggcggcac
    34201 caatcccgcc gcgtgcctct acatgaagaa cgacttcatc gagaagaacc cggaaacgac
    34261 gcagcgcctc gtgaatggcc tgttcaaggc gctgaagtgg ataggcagcg cctcggcgga
    34321 tgatatcgcc aattcggttc cggccgaata ccacctcggc gacaagggca tctacatgcg
    34381 ctcgctcgcg gcggttcgcg acagctactc gaagaacggc atcgtcagcg aggcggggca
    34441 ccagtcgatc cacgccatgc tcgtgcaact ggatgccgaa ctgaaggacg cgaagatcgg
    34501 gcgcgacaag acgttcgtcg agcggttcgt ggcaaaggcg agcgcctagg gcggcatcgg
    34561 gcgcgctccc tcgcctcggg tgagcgcgcc ggccgtcctg ccaggctgac ttgcgatgcc
    34621 gcgcgtcctg tcgatccagt cgcacgtggc ggacggccat gttggcaatg ccgccgccgt
    34681 cttgccgatg caacgcctcg ggatcgaggt gctgccggtg catacgctgc aactgtccaa
    34741 tcaccccgga cacggcgctt tccggggccg ggtcttcgag gcgtcgcatg tcgcggaagt
    34801 tctggaaggg ctggaagcgc atggcagtct tgcgtcctgt gatgccgtcc tgacgggcta
    34861 cctcggctcc gccgcgatcg ccgaggtggt ccgtgatgtc gtcgcgcgtg ccaggtcgct
    34921 gaacccgtcg cttgtctatg tctgcgatcc cgtgattggg gacgcgcaca aaggcgtgta
    34981 cgtccggccg gacctcgccg acgcgatccg cgaccggctg gtgccgctgg cggacttcct
    35041 gctgccgaac cagttcgaac tgcagtttct cacggggatc gacatctgcg acgcagcgtc
    35101 gctccgcgct gcggccgagc ggttgacggg cggcgtcaga agcgccgtcg tcacctccgt
    35161 gacgcatggc gggattgccg cgccctgtgt cgcgaccgcg ctgtttgacg cgcggggaac
    35221 cgaagtgatc gagacgcgcc gcatcgatcg ccagttcagt ggcgcgggcg actgcttcgc
    35281 cgcgctgttc acggcttatc ggctggtgac aggagacaac cgagcggcgc actggcaggc
    35341 ggcgaccgca atggccagcc tgctcagcaa gacggagcgg ctcgggcgcg acgaactggc
    35401 cctcgtcgag gggcagggcg cctttcgcct gcgcagcctg gccgaactca gccgggccca
    35461 ccgacaatgg aaacggccgg gctgagccgg ccgttccagg atgcgcaggg tcgaagtgac
    35521 gtggcgcggc tcgaaggcga cgtccggatg gacaccgcgc gccggccgaa tgctcttagc
    35581 ggaagcggac cttgtccttg atcgcggctt cggcgttgga cgggaccacg ttcttggcaa
    35641 cgaaatcatt ccagtccttg tagggacgct tggcgatgat gtcggcggcg cgcttctcgc
    35701 cgatctgcgg cagcttgtcg agttcagacg ccggggcggt gttgaggttg acgagctggt
    35761 tctgcgcagc gggctgcgcg gccggagccg gggcggt
//
LOCUS       DAOMXN010000010        34242 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_860_length_34242_cov_12.313950, whole genome shotgun sequence.
ACCESSION   DAOMXN010000010 DAOMXN010000000
VERSION     DAOMXN010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 34242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34242
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_860_length_34242_cov_12.313950"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..541)
                     /gene="nrdR"
                     /locus_tag="PK812_02145"
     CDS             complement(62..541)
                     /gene="nrdR"
                     /locus_tag="PK812_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HRJ68381.1"
                     /translation="MRCPYCGSLDTQVKDSRPADDNTSIRRRRVCPDCGGRFTTFERV
                     QLRELTVVKRSGRRVPFDRDKLSRSVHIALRKRPVPDERIERLINGIVRQLEASSDGD
                     VTSEQIGELIMEGLKGLDSVAYVRFASVYRNFREAREFGELIDSLEKDGETTPEQGA"
     gene            complement(591..1874)
                     /gene="glyA"
                     /locus_tag="PK812_02150"
     CDS             complement(591..1874)
                     /gene="glyA"
                     /locus_tag="PK812_02150"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HRJ68382.1"
                     /translation="MAAPRSNSFFSASLADRDPEIARAIDLELGRQRDEIELIASENI
                     VSRAVLEAQGSVLTNKYAEGYPGRRYYGGCQFVDIAEKLAIERACKLFGCSFANVQPN
                     SGSQANQGVFFALMRPGDTFMGLNLAAGGHLTHGAPVNMSGKWFNVVSYNVRPDTQTI
                     DMDEVEKLAKEHKPKIIVAGGSAYARHWDFARFREIADAVGAFFMVDIAHFAGLVAGG
                     AHPSPFPHAHVVTTTTHKTLRGPRGGMILTNDEEIAKKVNSAIFPGIQGGPLMHVIAA
                     KAVAFHEALQPEFKAYAQQVVDNAQALAKSLLGHGFALVTGGTDNHLMLVDLRPKKVT
                     GKAAEAALGRAHITCNKNGIPFDPEKPMVTSGVRLGSPAATSRGFGVSEFTTVGDLIA
                     EVVEGLSKHGDAGNGAVEEAVKAKVHQLTARFPIY"
     gene            complement(1999..2628)
                     /locus_tag="PK812_02155"
     CDS             complement(1999..2628)
                     /locus_tag="PK812_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HRJ68383.1"
                     /translation="MARTWGVSVFTSQTRLWLSAAAVLALAAPVAQAQTVNIVVIGDS
                     NVAGTGVSAEDRYPSQLEAALKARGLDVRVSNQGVNGEVSAATASRAGWIGSGTDIVV
                     YWNGCQNDRRHGMSLDACRSSNGSAMATLAGKGILTYLIRPPVHDQSMHGNKNFTLGG
                     EGKMMDFRDGRGAVPDGHFNRAGYAVITQRTLEPVRKLVLQAQKKKAGR"
     gene            2747..3121
                     /locus_tag="PK812_02160"
     CDS             2747..3121
                     /locus_tag="PK812_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68384.1"
                     /translation="MRRLWVLVFLLLAVPALAAGENCPVGGDASRAAIDAAPSCSAAM
                     KQVRDCPWGSGMDNILWRHAMDKCEAGVFARLTPAERRHLDRADAACTRKVARQSGTM
                     AVSAAALCRADAAMKLARRKKG"
     gene            complement(3139..3933)
                     /locus_tag="PK812_02165"
     CDS             complement(3139..3933)
                     /locus_tag="PK812_02165"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRJ68385.1"
                     /translation="MALGVEGSIARCLLVAALAVVMNASALAAGGTPPISSACETPLP
                     DIATPEPLPNLAASLERKLPLKVMAIGSSSTVGIGASAPSRSYPNQLSEILLRNYTDL
                     KLTMVNRGVSGELAAVTAERLRMQVALERPTLVLWQVGTNDALNRVPVEQFVQTVTNH
                     VRWMKSHDIDVVLVGLQYTAKSVRDEHYHAIRHALTELAEREGVLLVRRYAAMEFLEK
                     AKGTPVLTDDDLHLNDLGYRCMAEHIAHALVVSSFLKKPKRPPLMQ"
     gene            complement(3888..4976)
                     /gene="opgC"
                     /locus_tag="PK812_02170"
     CDS             complement(3888..4976)
                     /gene="opgC"
                     /locus_tag="PK812_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018264903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OpgC domain-containing protein"
                     /protein_id="HRJ68386.1"
                     /translation="MRAPNAIDFWRGFALVSIFINHVPGLAYERLTHRNIGISDSAEL
                     FVFLAGWSLRGIAAGGGDPLTVRRTILRVGARGVTLYAAQIVITMLAIALLAATALVV
                     QHQALLEWHNAASVFHDPIRTHVGLVLLTHQLGYFDILPLYVVLMFGAVLLALVHRLN
                     AGLVLPLSLALYVFALVSQVNFPTWPLEGVWFFNPLCWQLIFVLGFVAAGDNGIGAWL
                     HRNPRKVLALGLPGFLVGLVIAAIGYNPDPLTLPQPLLFFVIDKTYLTPLRLWHALSL
                     FAVMAAAFPLILRFFPVVVGFLCLLGRNSLYVFCVGSLASLAGQLARFVLGGNLAVDT
                     VILASGIVILGVTGWLSELKDRLRGASS"
     gene            5091..5702
                     /locus_tag="PK812_02175"
     CDS             5091..5702
                     /locus_tag="PK812_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68387.1"
                     /translation="MHRLPALAVLTLAVTAAAAHEAPDLDRMFPVGGDFCFARAYDAA
                     HMAKHPRQVVRAIQVMGRNAHRTAAAPGETAQERTVPGLNVRASLRVTFRDGAVRDWP
                     GTCHEDGDLPGHPAVCRFVPARNPDTLEFGLRLKRTGAAVDAVAFGDIGRFRRALDEG
                     SDRRRDSDDRVFRLHASTSATCAFPAKYWSARGATRALEIALP"
     gene            complement(5707..5946)
                     /locus_tag="PK812_02180"
     CDS             complement(5707..5946)
                     /locus_tag="PK812_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HRJ68388.1"
                     /translation="MKKIPARHTWLVMPAILALLMTFVVSCVSTLRAIGLAPDFASKW
                     MQAWLLSYVVAYPTLLAVMPVVRRLVAAIVEPPKA"
     gene            6052..6504
                     /locus_tag="PK812_02185"
     CDS             6052..6504
                     /locus_tag="PK812_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRJ68389.1"
                     /translation="MTSVPSVPPQAGVLPGAALDGVRSRRIVAWMIDFVIICALMWLF
                     GLIMVVLSLPTFGVSFALAPLIAFVGVFYSGLTVSGRGRGTWGMRALDLQVTARDGSR
                     VDFIVAAGHALLFYLLTPFFTLLSLFNAEKRTLHDFLTGVIVTRRPPG"
     gene            6585..7304
                     /locus_tag="PK812_02190"
     CDS             6585..7304
                     /locus_tag="PK812_02190"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HRJ68390.1"
                     /translation="MTQQPRDTPQFYLTSPTSCPYLPGRQERKVFTHVVGKRAPELND
                     LLTHSGFRRSQTIAYRPACESCRACVSVRVLARDFEPTRNMRRILKANTDLVVAVVPP
                     KPTSEQYSLFRAYLDSRHFDGGMADMSVLDYAMMVEDTHIDTRLVEYRSTARDNALIG
                     VALTDRLGDGLSMVYSFYDPDEAERSFGTFMILDHIRRAADQDLPYVYLGYWVDGSRK
                     MDYKARFQPQERLVGEGWKRA"
     gene            7420..9636
                     /locus_tag="PK812_02195"
     CDS             7420..9636
                     /locus_tag="PK812_02195"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRJ68391.1"
                     /translation="MGSIPDDNATTFNHVDPGTKLALCLSGGGLRATFFHFGVVKALA
                     GADRLKDVSDIIAVSGGSILAGHMIKNWDSYCGTAQNLDAMEMELRRMAARGMRERVV
                     RRWVTKGAWHGLKALLGSEHRPYTQSRAIQYEYERVLGKGTVADCYRNAPREKRPPQV
                     HFMCTNFNSGEPCCFTSDEFIIYRKPPISVVNGVAKRPAGAGHTGTGSDKDEPVRKLT
                     MAVSSDTVELSYAVAASSAFPGLFPPMKLNAARLGASARDFPVTVYLSDGGIYDNLGL
                     EHFKELVRQSEKKASSETSSQVRAKPDDERMLKASTGGTHAQAVLVSNASGAFQWDNR
                     NDFYHLLPRNLRTIDILMNLMIEERWETTPKELVQGEKARKIHLLRADIGNAHWEAGA
                     PPKSVQQQIQDIRTDLDRFTDEEITLLIDHGYRTARQQTGVEPVQQSSPFMAAAAGEP
                     APVGRFRLLDDLVKTAAERQPRLFDWHDPLRSLGMGIALLVLFVAAILPFVAPFAAWG
                     WWDANEKLFDSRKENDAKSDRIAAQAQELQSIKKALEDITNAQIASATRLEQLSGSSG
                     AKAPPPDSSTPADLPVLRPDPTRPSRDVLLSQREKLDELQKQIQFTIKQEVARIETLS
                     AQRDFKPQSTRVEVNRQSYKVWLQFAGSFRRQEMIDFGKALKAKWPNAPGAEQGGERT
                     ENAAGLFEVRYGPPEDKAAAEALTQDILDLKIVPKMNQPRRLAIIPKGSLEIWISK"
     gene            complement(9643..9921)
                     /locus_tag="PK812_02200"
     CDS             complement(9643..9921)
                     /locus_tag="PK812_02200"
                     /inference="COORDINATES: protein motif:HMM:NF025051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HRJ68392.1"
                     /translation="MKLIAAASASLAALAGVGVSLWPQTCTSTPPRHWLTPLEVEARL
                     RESGLKLQSLRTGDDRCFAVVARDRQGRLRDLVVNPADGMIVREAERG"
     gene            complement(10099..12342)
                     /gene="parC"
                     /locus_tag="PK812_02205"
     CDS             complement(10099..12342)
                     /gene="parC"
                     /locus_tag="PK812_02205"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HRJ68393.1"
                     /translation="MGKPVEPPKDGEVESVELKQALEERYLAYALSTIMHRALPDARD
                     GLKPVHRRLLHAMRLLKLDPGAAFKKCARVVGDVIGKYHPHGDQAVYDAMVRLAQDFA
                     QRYPLVEGQGNFGNIDGDNAAAYRYTEARLTDVAALLLEGIDEDAVDFRPTYNQEDEE
                     PIVLPAAFPNLLANGAQGIAVGMATSIPPHNAAELCQAALHLLENPGAAPDDLLRFVP
                     GPDFPTGGVIVEGRGSIAETYRTGRGGFRVRAKWEKEDLGRGTWVVVVTEIPYLVQKG
                     RLIEKIAELLNEKKLPLLADIRDESAEDIRIVLEPRSKTVDPVMLMESLFRLSELESR
                     IPMNMNVLVDGLAPRVIGLAEALRCWLDHRRDVLLRRSRHRLGKIEHRLEILGGLLIV
                     YLDLDEVIRIIREEDDAKAALMARFTLTELQANAILDTRLRSLRKLEEMELKREHEAL
                     TKEKAQIEQLLGSESKQWKTVAWQVKDVLRSFGPETRLGRRRTQFAEAPATSEIDLTE
                     AMIEKEPITVVVSEKGWIRALKGHVVELANVQFKGDDSVKLSFPTETTAKLLVLTTNG
                     KVFTLEASKLPGGRGFGDPIRLMADIDPEAEIVTVWPHEPGAKRLLIARDSRGFVANS
                     DDLVANTRKGRNVLSVDSARAWLSVPVSGDTVAIVGENRKLLVFPLDQIPEMARGKGV
                     RLQKYKEGGVSDAKTFTLADGLTWLDSASRTYTVKGAELAEWQGNRAESGRLPPKGFP
                     KNNRFTG"
     gene            12475..12696
                     /locus_tag="PK812_02210"
     CDS             12475..12696
                     /locus_tag="PK812_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68394.1"
                     /translation="MPDKMNRPKEIIEFCLKPLNLDKNSDAYEAAYARLEHVIKTYQA
                     QILKGKQSVTVDFSQMKTITINEDAHGHD"
     gene            complement(12763..13482)
                     /gene="recO"
                     /locus_tag="PK812_02215"
     CDS             complement(12763..13482)
                     /gene="recO"
                     /locus_tag="PK812_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HRJ68395.1"
                     /translation="MEWRDDGIIIGLRRHGEANVILEAMTRAHGRHLGLVRGGRGRRQ
                     APLLQPGNAVDLTWRARLDEHLGTYAVEVTQVRAARFLGSAAALYALNTVAEHLRLLP
                     ERDPHGPLFEALDVIIAHLDEPATACALIARFELALLGDLGFGLDLETCAATGSNEDL
                     VFVSPKSGRAVSRVAGEPYADRLLPLPCFLKPRAASQQCAPQLAEAFRLTGYFLERHV
                     LQPRGLAMPESRAALLTLATG"
     gene            complement(13493..14398)
                     /gene="era"
                     /locus_tag="PK812_02220"
     CDS             complement(13493..14398)
                     /gene="era"
                     /locus_tag="PK812_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HRJ68396.1"
                     /translation="MTAPVTRCGFVALVGAPNAGKSTLLNALVGAKVSIVSRKVQTTR
                     TQVRGIALSGQAQIVFVDTPGLFKPKRRLDRAMVTSAWGGAGDADMVGLLVDVHKAGE
                     DETLAILERIADLRQPVFLVLNKIDTMPRDRLLAVAADLNAKAKFAATFMVSALTGDG
                     VATILTWLEGRLPPGPWLYPEDQISDAPLRFLAAEITREKMFERLHDELPYRSTVETE
                     QWKTLRDGSVRIEQTIYVERESQRKIVLGEKGATIKKIGELARKEIAEAAEAKVHLFL
                     FVKVRENWADDPERYREMGLEFPKG"
     gene            complement(14395..15099)
                     /gene="rnc"
                     /locus_tag="PK812_02225"
     CDS             complement(14395..15099)
                     /gene="rnc"
                     /locus_tag="PK812_02225"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HRJ68397.1"
                     /translation="MAADAARLAELEERLGHVFTDKSLLEQALTHVSHSGAGTKAMSY
                     QRLEFLGDRVLGLCVADLLYARHPEAPEGQLSRALADLVRKETCAAVAESIGVGACLR
                     MGKGERKSGLARKPAVLGDACEALLGAVYLDAGLPTVDTVVRRHWLERLPDMTTAGVD
                     PKTALQEWAHTRGLPEPRYAEVDRTGPDHAPVFRVAALVEGLEPCEGQGRSKRIAERA
                     AAEAMLAREGVAGGMP"
     gene            complement(15101..15853)
                     /gene="lepB"
                     /locus_tag="PK812_02230"
     CDS             complement(15101..15853)
                     /gene="lepB"
                     /locus_tag="PK812_02230"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRJ68398.1"
                     /translation="MSDKSKSEGGIGETIRVIIEALLIALVVRTFLFQPFNIPSGSLI
                     PTLLVGDYLFVSKYSYGYSKHSFPYSAVPTNGRYFFVSEPKRGDIAVFKLPMDGQTDY
                     IKRVIGLPGDRVQMIQGVLHINGSAVKRELVGTYPGNDKFGRPAQVPRYRETLPGGGS
                     HEIIELEGDTSYWDNTDVYHVPEGHYFMMGDNRDNSTDSRVLNQVGYVPFENFVGRAE
                     IIFFSIDENVRFWEVWNWPWAIRWSRMFRGIR"
     gene            complement(15970..16371)
                     /gene="acpS"
                     /locus_tag="PK812_02235"
     CDS             complement(15970..16371)
                     /gene="acpS"
                     /locus_tag="PK812_02235"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009757553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HRJ68399.1"
                     /translation="MIIGIGSDLCDIRRIEKSLDRFGDRFVNRVFTEVEKAKSDRRSE
                     RAASYAKRFAAKEACSKALGTGIRMGVFWKNMGVVNLPSGKPTMRLEGQALERLHALT
                     PSGYEPVIHLTITDDYPLAQAFVIIEARPVA"
     gene            complement(16368..17120)
                     /locus_tag="PK812_02240"
     CDS             complement(16368..17120)
                     /locus_tag="PK812_02240"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011927321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HRJ68400.1"
                     /translation="MSGTPIRLGVNIDHVATVRNARGGAVPDPVRAALAAIEAGADGI
                     TAHLREDRRHIRDDDVFRLKAEIAKPLNFEMANTVEMLDIALRVKPHACCLVPEKREE
                     RTTEGGLDVAGAGPDLAMAIARLSHAGIRVSLFIEPDAGQIAAAKRLGAHIVELHTGT
                     WCEAAVHHEVEKAEREYQRLAEGARLAAQLGLEPHAGHGLDYRTAEKLAHVREFVEFN
                     IGHFLVGEAIFVGLGDTIRQMKAAMLRGRKAR"
     gene            complement(17262..18350)
                     /locus_tag="PK812_02245"
     CDS             complement(17262..18350)
                     /locus_tag="PK812_02245"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRJ68401.1"
                     /translation="MDQHCPGAYFDGVSSRRRDVTVRIDAGLEIIDGDVCLAEWSFLD
                     LRQLDSLPDTLKVRSVAAPELASLTIADPTTIEAIRVRAARLRYDGQEPRHGILRIVA
                     LSLAAAVSILGVAFFGVPKMARAVASITPVWAERKLGDAVAGQVKVMFGGEVCVGGEG
                     GAALAKLSEKLTVKARLPHPVRIEVLSSRIPNAVALPGGRVFLFDGLLARAEHPDEVA
                     GVLGHEIGHEANRDGLRALIEAGGTSFLLGLLFGDLTGSATLVLVVREIISSAHSREA
                     EAAADIFGRDLMHALGRPAAPLARVLTRIDPSKPGAKGSIFDSHPLTAERLKRLEAAD
                     ASLGRPLDGPPLLNEAEWAALKTICAKK"
     gene            complement(18350..19603)
                     /locus_tag="PK812_02250"
     CDS             complement(18350..19603)
                     /locus_tag="PK812_02250"
                     /inference="COORDINATES: protein motif:HMM:NF017770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HRJ68402.1"
                     /translation="MTSESDPEGPWGPRKADLKPPPPTESAPQTKPATSADPAPSREE
                     VTPVIFHGSRRDWVGIMARGMALYLPTFGFYRFWLKTEQRRFLWRSISVNDEALAYHG
                     TARELLVGFLFAIAVYLPISVTYFILGLYAETVQAFASVPFALFTIVFSYFAAYRARR
                     YRLTRTVFRGVRFWMDGSAWRFAFTALGWMVATGVTLGIAWPWMAAGLERFKWRHSHF
                     GDLQGDFHGTGWGLFKALFWPALGLVVGAFIPIANLLAIPIMMSAIIAVYGHWLIGGV
                     SFGPVRFQSSIDSFLLLGPAVVTALAVGLFGAVWIGIGYVGFWGSLLGLASFEVPQGS
                     DAAVLGALVRHAPWLLLGFAWGLIGLVGITFLKSLFFDFPRMQRFAASTSLTNAEALE
                     TVVQKGHAEGSLGEGLADALGSDGF"
     gene            complement(19738..20574)
                     /locus_tag="PK812_02255"
     CDS             complement(19738..20574)
                     /locus_tag="PK812_02255"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRJ68403.1"
                     /translation="MKSILMCLHAPFAENVHPPACSAYGISLASKLGAHLSVVMAAPK
                     LQQSVFLPVPVVTGLLADNDRVRAANAATMSEALGRSAVASGITMSTETLQSPFPSLQ
                     RRLLNRAHIHELVLVGRPGADDYVMPGLIESLIFESGRPVMLVDSDHARPAAFERIVV
                     AWDGSARAGRAVWDALPLLKQAQKVEIVSIKDEKDLSKSVPGAELAPALARHGIDVTV
                     TDLMCKGDTVANTIKAHVGLVRADLLVMGAFGHNRWRELILGGVTEDLLRAANIPVLM
                     SR"
     gene            complement(20655..22715)
                     /locus_tag="PK812_02260"
     CDS             complement(20655..22715)
                     /locus_tag="PK812_02260"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HRJ68404.1"
                     /translation="MDRFVDSVADAVAKKLAGEGMASAQAAAVRKNAEETSETIHQLV
                     SRLGVIASQFPGLVRHVAGLPARLDASGPKDAGFIGLLIKIAAVAGLALAAEWAARMA
                     SSGLVKRIVRSVESPPPWARWFALTAVEAGAVVAFAVVGNAVFASLFLSGGEHGRIAG
                     LVLMGLFWWRVGLAVARCWLRPHLPAGRLAPVDDHDAAVLMRGFAMATAVPSITRAFA
                     GILVATNAQRDEIVLYQFLFAPLVVGTFIAMVLAVRMPMARWLARLGSLNDERSWRRV
                     LADNWHVPGVLFYVLVWAAQAYGGMSGRVDVPASLNDLQQLLIGWLLVETILFCIHRR
                     RLDAKKSAGITAPQLEDIAWRGLNILVRIAIFLIAVRIVVTEILNLIPHSAWAGYSAT
                     LAGAGFVVFAAWAIWQVTKFYFEKVAGPARAPAIPGAENETEEDTLEGASRLQTLMPV
                     LRSVLGVLIVGFTVLIVLSQLGVNVTPLLAGASIFGLALSFGSQSLVRDIVSGIFFLA
                     DDAFRVGEYIDIGKAKGTVESFTLRSIRLRHQGGQIHTIPFGQLGQITNFSRDWATMK
                     FNLRFARETDIEALRKATKKIGQELLEDPDLKDDFLLPLKMQGIADIQDNALIARFKF
                     TVKPGRPSYIQRIAVKRMLQVFPEKGIEFASATVAVKSLDQGGVLPELAAAAARGAPA
                     PVGG"
     gene            22941..23363
                     /locus_tag="PK812_02265"
     CDS             22941..23363
                     /locus_tag="PK812_02265"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRJ68405.1"
                     /translation="MREPAPSLDSLPFRTQHELRYSDMDEQGHINNGVSSALMEHCRV
                     SLLRGGGLKARFNNTFVLARNEIDYFAELFWPGQVTAAIGVERLGETSITFRQAIFRD
                     GSCISAGRAVVVAFDGQTRRPTPLAMEARRELEAWRVR"
     gene            23547..24452
                     /locus_tag="PK812_02270"
     CDS             23547..24452
                     /locus_tag="PK812_02270"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRJ68406.1"
                     /translation="MPSLAAPPAPPRWKVVLILMLIATTFGSNHVAARFAFDHGASVF
                     TAALVRSLGTALALVVALKATGVSLKVPRHQVFRAAVTGLLVASASVFIYSAVARIPV
                     GLALLTFNTFPFVFTLVTWLMDGRRPSGRTLGFMGLALAGLSLALTFGGGASYPPPAV
                     MATGVALAFAASLSFGGVLYLSQRWLGAMDGRVRTLIMMSTTAIVVGVIGTLGDAFRL
                     PVDSVGWTGLALLTILYGSAFTALFVVLPRYGMLDNAGVLNFEPVAVLAMGAVLLGQT
                     ITPVQMLGAGIVLVAMLGFASEGKR"
     gene            complement(24463..25908)
                     /locus_tag="PK812_02275"
     CDS             complement(24463..25908)
                     /locus_tag="PK812_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRJ68407.1"
                     /translation="MSIFPNFIGGETVSSPATSRNVNPSDTNDVIGEFAAGSRADVEA
                     AIAAAKAAFPAWSRTTPQERYDVLKKASDEVLARREELGRILSREEGKTLPEGIGEAT
                     RAGQILAFFAGEALRIGGETVPSVRPGLGVEMTREAVGVVGMITPWNFPIAIPSWKIA
                     PALAWGNTVVLKPADLVPHSAWALCDILIRSGLPKGVLNLVMGRGSVVGQAIIDHADV
                     AAISFTGSVSTGRRIAASCVASQPMKKVQLEMGGKNPLVVLDDADLKTAVECAVNGAF
                     FSTGQRCTASSRLIVTKGIYNAFVAAMVERMKGLVVDDALKAGTHIGPVVDQSQLDQD
                     EKYIAVGRDEGAKLAWGGERLNRDTPGYYLQPALFTDCSNAMRISREEIFGPVASVIP
                     ARDYDEALALANDTEFGLSSGICTSSLKYATHFKRNAEAGMVMVNVPTAGVDYHVPFG
                     GRKRSSYGPREQGSYAREFYTTVKTAYTLAM"
     gene            26298..26891
                     /locus_tag="PK812_02280"
     CDS             26298..26891
                     /locus_tag="PK812_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68408.1"
                     /translation="MAQLLSLIVLIVVVFGIVNFVHFRLLPVRVVLYDAVLDLVVAFV
                     ICLLLYGLFRRSWGSLSGMEAMLAMAVGFLIGLFYAISIPTVVDRSLSIYILEKVAQR
                     GGAIREDALGDIFINEYMPEHRLVDIRLTEQLNSATITVENGCIRLTERGRRIAEMTR
                     WYRAHILPRHRELRGELTDALTDPFRHSKTDVTYQCR"
     gene            complement(26983..27945)
                     /locus_tag="PK812_02285"
     CDS             complement(26983..27945)
                     /locus_tag="PK812_02285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRJ68409.1"
                     /translation="MVNEANTGRKPHHALIVIMPNYEDRASATHLLRDLARECAVAPY
                     VVAIDDGSTRDPLGCEAIQAAGLAGEVVYLRRNMGHQRAIAVGIVYVAANFSADAVIV
                     MDSDGEDQPHSIDPLLTTLNEGRYDIVVAERRRRSEPWSFRTFYFVYRQLFRLLTGRS
                     IRFGNFSALSMRAVARLAAMQETWVHVAAAVMVSRLRIGSVATDRGTRYEGRSRMNFT
                     SLALHGLRSIIVFAEDVLVRVGISCILLSALALGSLVATVVLKTIGFATPGWFSIAVG
                     VLVLIVMQAGILTFVTLMVSGFIRNAPPASQATLDQLIERVDRA"
     gene            complement(28037..29872)
                     /locus_tag="PK812_02290"
     CDS             complement(28037..29872)
                     /locus_tag="PK812_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68410.1"
                     /translation="MPKGGREVRKISLALGISAILAVLFVNRFAPEKLDADLILMSIM
                     SLQNVTLFYWGQNRLANVLPLAVSFIRDPAANLFALMVLATAAHFVLLFLIARGVSQF
                     LRLGGHLRLAEFQIFAVLCLATLLLIEESVSYALIIAHPEYSMAAALIGIALILAVQA
                     GALRSIRWALAALCIFVAIGINFAAVIPMTYAAGIVALMAARFRLRAIAVVGMTMASA
                     YAWSLLSAAHGQSRYAGFSLQGFRDGILPTLSNLRLDFDPAGAAFVAVIIVFGALSLF
                     RHWGRQAEMNGRIAFVFAAVVAFSIGWIVLFSSNEWVATNGHASRYFVFATYGLVLLC
                     AFGIAGALTVGRSGRWEVVTFAALAFAIAALVRSPVAFSDYAVFRKVNVAVPQPVGLY
                     AGDYWVAWPAVMRDLLADRSAYGLGYRAEGNGAAARAAVAAMAASAGAFPVVCLEAAQ
                     AECRRQVSAVVGPVDVVQASAGAPSGTAPVLAFALATAISYRGAELGSLPSKSGAFTG
                     THLETNGTAGCLFFGPYTRAWAGRYRLVVEGRATRLAEAVVDVTSRTGRVQHVAFALN
                     GPGEEGRLLDREVLLAEEISDLEIRLCVGSQDVLSVHGYTFAKIP"
     gene            complement(30422..30497)
                     /locus_tag="PK812_02295"
     tRNA            complement(30422..30497)
                     /locus_tag="PK812_02295"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30462..30464),aa:Phe,seq:gaa)
     gene            complement(30597..30800)
                     /gene="yacG"
                     /locus_tag="PK812_02300"
     CDS             complement(30597..30800)
                     /gene="yacG"
                     /locus_tag="PK812_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013164967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HRJ68411.1"
                     /translation="MSEDPAPFPANDNARPCPVCGKRPVDRYRPFCSARCADVDLGRW
                     LAGAYAIPAEEEDEGLPERGEER"
     gene            complement(30803..31441)
                     /locus_tag="PK812_02305"
     CDS             complement(30803..31441)
                     /locus_tag="PK812_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf-like protein"
                     /protein_id="HRJ68412.1"
                     /translation="MTAVTDGWRPRLALASASPRRFALLQQIGVVPDAVVPAAIDETP
                     QRGEKPRALVTRLSREKAKAALKVVRSDPALAGAFLVAADTSVSVGSRILPQTHTVDE
                     AAHCLRLLSGRSHRVYTSVVVVTPGDAFRQRIAEARVRFKRLSESEIEAYLGSGEWRG
                     KAGGYAIQGLAGAFVVKLVGSYSAVVGLPLYETAGLLVGDGFPLHFSWANSV"
     gene            complement(31476..31694)
                     /gene="infA"
                     /locus_tag="PK812_02310"
     CDS             complement(31476..31694)
                     /gene="infA"
                     /locus_tag="PK812_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HRJ68413.1"
                     /translation="MSKEELLEFPGIVIELLPNATFRVKLENDHEIIAHTAGKMRKNR
                     IRVLAGDKVLCEMTPYDLTKGRITYRFK"
     gene            31997..34105
                     /locus_tag="PK812_02315"
     CDS             31997..34105
                     /locus_tag="PK812_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRJ68414.1"
                     /translation="MSPLPPITFDVAIVGGGPGALMAAMHINEKVGAEAKVTIFEQSN
                     RLGGKLMTRSFDKVPALYEAGVAEIYDYAHIGPDPLKELITGKLGLDVVRMDSECVVI
                     GDKIINTYAEIERHLGKKASDAIAKFRKKCTTILSPQQYYEGIGRDDNDQEWLWKSGL
                     DVLHDEVKDETARNYIRYNAHSDIAIAPNETSGLNACKNFLMDWDEYIKYYSVVGGNE
                     RITQRLSEIVRADVEMHSRVRRVGKAPNGKYRLVVQQNGKEVERLFDFVVVSLPYNWL
                     ATMEWEGERLYRALGAHLDHFNYPAHYLRVAILFEKPFWKDFVQGSWFMSDAFNGCCV
                     YDESSRHDYGKAGALGWLIAGSDALAYANMPDDHLVRMALASLPPQLQFGRELMLEAK
                     VVRWLNTVNGIPGGRPVRDTQQNHQPEPVEHPGLFVTGDYLFDATLNGVLDSADCASD
                     MLMSEYVKADYEARIRLRTRGAEAAKRTAVASSRNVNRAYFENYWNHGPYPQAWNKFF
                     HAGILRDSIRDVWGAGKTFSMLDAGSASGLTVQALRNLGLDAWGIEKNAWIHAQTPKD
                     VRAYNKLGDVTELPFKDGQFDFVYESCLANLPRHKVQKAIRELHRVARKGIILGSVTS
                     DLTWPVLIDNEMQRGVKTFATLWQWSDWLFNVGFDHAIEDDALLKRIWDRHDAVGMGK
                     GGWFEDPESIRYCYFNRLDV"
BASE COUNT         6570 a        11066 c        10818 g         5788 t
ORIGIN      
        1 gccgcgtcca tcaggcgatg gtcgagatcg tcggcaggcc acgccggcgc gccggcagga
       61 ttcacgcgcc ctgctccggc gtggtttcgc cgtccttttc cagcgaatcg atgagttcgc
      121 cgaactcgcg cgcctcgcgg aaattgcgat agaccgaggc gaagcggaca taggcgacgg
      181 aatcgaggcc cttcagcccc tccatgatca gttcgccgat ctgctccgag gtgacgtcgc
      241 cgtcgctgga cgcctcaagc tggcgcacga tgccgttgat cagccgctcg atgcgctcat
      301 ccggcaccgg acgcttgcgc agcgcgatat gcaccgatcg cgacagcttg tcgcgatcaa
      361 acggcacgcg ccggcccgag cgcttgacca cggtgagttc gcgcagctgc acccgttcga
      421 acgtggtgaa gcggccgccg caatcgggac agacgcgccg gcggcggatc gacgtgttgt
      481 cgtccgcggg gcgcgaatcc ttcacctgcg tgtcgagcga gccgcaatag gggcagcgca
      541 tgggggctcc ggtcaggcgt cctggcccgc ggcgcgcggg ccggaaaacc tcaatagatc
      601 gggaagcgcg ccgtcaactg gtggaccttg gccttcaccg cctcttccac cgcgccgttg
      661 ccggcatcgc cgtgcttcga caagccttcc accacctcgg cgatcaggtc gccgaccgtg
      721 gtgaactcgg agacgccgaa gccgcgcgag gtcgccgccg gggagccgag gcgcacgccg
      781 gaggtcacca tcggcttttc cggatcgaag gggatgccgt tcttgttgca ggtgatgtgg
      841 gcgcgcccga gcgccgcttc cgccgccttg ccggtgactt ttttcgggcg caggtcgacc
      901 agcatcaggt gattgtcggt gccgccggta accagcgcga agccgtggcc gagcaggctc
      961 ttggccagcg cctgcgcgtt gtccaccacc tgctgcgcgt aagccttgaa ctccggctgc
     1021 aaggcttcgt ggaaggccac ggccttggcg gcgatcacat gcatcagcgg accgccctgg
     1081 atgccgggga agatcgccga attgaccttc ttggcgatct cctcgtcgtt ggtgaggatc
     1141 atgccgccgc gcgggccgcg cagggtcttg tgcgtcgtcg tggtgacgac atgggcatgc
     1201 gggaagggcg aaggatgcgc gcctcccgcc accagaccgg cgaaatgggc gatatcgacc
     1261 atgaaaaagg cgccgaccgc gtcggcgatc tcccggaacc gggcgaagtc ccagtgtcgg
     1321 gcataggccg agccgccggc gacgatgatc ttcggcttgt gctccttcgc cagcttttcc
     1381 acctcgtcca tgtcgatggt ctgggtgtcg ggccgcacgt tgtaggagac gacgttgaac
     1441 cacttgccgg acatgttgac cggcgcgccg tgggtgaggt gtcctccggc ggcgaggttc
     1501 aggcccatga acgtgtcgcc cgggcgcatc agggcgaaga atacgccctg gttggcctgc
     1561 gagcccgaat tcggctgcac attcgcgaag gaacagccga acagcttgca ggcgcgctcg
     1621 atcgcgagct tttccgcaat gtcgacgaac tggcagccgc cgtagtagcg ccggccggga
     1681 tagccttcgg cgtatttgtt ggtcagcaca gagccctgcg cttccagcac ggcgcgcgag
     1741 acgatgttct ccgaggcgat gagttcgatc tcgtcacgct ggcgaccgag ttccagatcg
     1801 atcgcccggg cgatttccgg atcgcggtcg gcgagggagg cggaaaagaa ggaattggag
     1861 cggggagcgg ccatgggcgc ctcgtcaatc agtcgcgact tcgcggagga acgggtttcg
     1921 gccggcgctt agcatgcgcg gcaagcgggg ggaaggccgt gttcgcgagg tgcgaagaga
     1981 tgacggctgc gacgcggcct agcgtcccgc cttcttcttc tgcgcctgaa gcaccagctt
     2041 gcgcaccggc tccagcgtgc gctgcgtgat cacggcatag ccggcgcggt tgaagtggcc
     2101 gtccggcacg gcgccccgac cgtcgcggaa atccatcatc ttgccttcgc cgccgagcgt
     2161 gaagttcttg tttccgtgca tgctctggtc atgcaccggc gggcggatca gataggtcag
     2221 gatgcctttg ccggcgagcg tcgccatggc gctgccgttg ctggaccggc aggcgtcgag
     2281 agacatgccg tgccgccggt cgttctggca gccgttccag tagacgacga tgtccgtgcc
     2341 ggaaccgatc caaccggcgc gactggcggt ggcggcgctc acctcgccat tcacaccctg
     2401 attggagaca cgcacgtcga gaccgcgcgc cttcaacgcc gcctcaagct gcgagggata
     2461 gcggtcctcg gcggaaacac ccgttccggc cacattgctg tcgccgatga ccacgatgtt
     2521 caccgtctgc gcctgcgcga ccggcgccgc gagcgccaga acggcggcgg ccgaaagcca
     2581 caggcgtgtc tgcgaggtga agacgctgac gccccatgtc cgtgccatga ccaacctcca
     2641 tctttccggc ttccccgccg ggcagtgaga cgctactcct ctgcgcagcg gccgtaaatg
     2701 gaccccgggg cgacggccgg aacacagtct tgcgatgagg ccgcgcatgc gacgattgtg
     2761 ggtgctcgtc tttctgctcc ttgccgttcc ggcgctggcg gccggcgaga actgcccggt
     2821 cggcggcgat gcgtcgcggg ctgccatcga cgccgcgccc agttgcagcg ccgcgatgaa
     2881 gcaggtgcgg gactgcccct ggggttcggg catggacaac atcctgtggc gccatgccat
     2941 ggacaagtgc gaggccggcg tcttcgcgcg cctgacgcca gccgaacgcc ggcaccttga
     3001 tcgggcggac gccgcctgca cgcgcaaggt cgcgcggcaa tccggcacga tggcggtttc
     3061 cgccgcagcg ctctgccgcg ccgatgcggc gatgaagctg gcccgccgca agaagggcta
     3121 gcaaaccgga aagcggcact attgcatcag cggggggcgt ttgggcttct tgaggaagga
     3181 actcaccacc agggcgtgcg caatgtgctc cgccatgcag cgatagccca gatcgttgag
     3241 atgcaggtcg tcatcggtga ggaccggcgt tcccttggcc ttttctagga actccatcgc
     3301 ggcatagcgg cgcaccagca ggacgccttc cctttccgca agttccgtca gcgcgtggcg
     3361 gatggcatga tagtgctcgt cgcgcacgga cttggcggtg tattgcaggc cgaccagcac
     3421 cacgtcgatg tcgtgagact tcatccatcg gacgtgattg gtgacggtct ggacaaactg
     3481 ctcgacggga acgcgattga gcgcatcgtt ggtgccgacc tgccagagca ccagtgtcgg
     3541 gcgctcgagg gcgacctgca tgcgcaggcg ctcggcggtc acggcggcga gctcgcccga
     3601 tacgccgcgg ttgaccatgg tcagcttcag gtccgtgtaa ttgcgcagca aaatctcgga
     3661 aagctggttt ggataggagc gcgagggggc ggaagcgcca atgcccaccg tggaggatga
     3721 gccaatcgcc atcaccttga gaggcagctt gcgttcgagc gaagcggcaa gatttggaag
     3781 cggttcaggc gtggcaatgt ccggcagcgg cgtctcgcag gcagaactga tcggcggggt
     3841 gccgcccgca gcgagagccg aggcgttcat caccacggca agtgccgcta cgaggaggca
     3901 ccgcgcaatc gatccttcaa ctccgagagc catccagtca ctcccaagat cacaatcccg
     3961 ctcgccagga tgacggtatc gaccgccaga ttaccgccaa ggacgaacct tgccaactgg
     4021 ccggccaggc tcgccagcga tcctacacag aagacatata acgaattgcg gcccaaaagg
     4081 cacaaaaaac cgacgactac ggggaaaaaa cgcagaatga gcgggaatgc cgcagccatc
     4141 acggcgaaca ggctgagcgc atgccacagg cgcagcggcg tcaggtaggt cttgtcgatc
     4201 acgaagaaca gcagtggctg cggcaaggtc aggggatcag gattgtagcc gatggcggcg
     4261 atcaccagtc cgaccaggaa ccccggcaga ccgagggcca gcaccttgcg cgggttgcgg
     4321 tgcagccagg cgccgatacc gttgtcaccg gccgccacga agccgaggac aaagatcagc
     4381 tgccagcaca ggggattgaa aaaccagact ccctccaatg gccaggtcgg aaagttgacc
     4441 tgcgacacca gcgcgaagac gtagagcgcg agcgacagcg gcaagaccag ccccgcgttc
     4501 agccgatgca cgagcgccag caggaccgca ccgaacatca gcacgacgta gagcggcaag
     4561 atgtcgaagt agccaagctg gtgcgtgagc agcaccagac cgacgtgcgt gcggatcgga
     4621 tcgtggaaca ccgaagcggc gttgtgccat tccagcagtg cctggtgctg aacgaccagc
     4681 gccgtcgccg ccaggagcgc gatcgccagc atggtgatga cgatctgggc ggcatagagc
     4741 gtgacgcccc gcgctccgac acgcaggatg gttcggcgca cggtcaacgg atcgccgccg
     4801 ccggcggcga tgccgcgcag cgaccagccc gcgaggaaaa cgaaaagctc cgctgaatcg
     4861 gaaatgccga tgttgcgatg cgtcaggcgc tcgtacgcga gcccggggac atgattgatg
     4921 aagatgctga cgagcgcgaa accgcgccag aaatcaatcg cattgggcgc cctgatggac
     4981 acgtcccgct ccagtcacct ggccgtcgcg ctggcggtca cgcggtgtga cgctgcgcac
     5041 ggcgcaggac tccgaatgcc accaaattcc gaaacatcaa gggcaatcgc atgcatcgac
     5101 tccccgctct cgcagtgttg accctcgccg tcaccgccgc cgccgcgcac gaggcgccgg
     5161 acctcgaccg gatgttcccg gtcggcgggg atttctgctt tgcccgagcc tacgatgccg
     5221 cgcatatggc gaaacatccg cgccaggtcg tgcgcgccat tcaggttatg ggacgcaatg
     5281 cccatcgcac cgctgcggcg cccggcgaga cggcgcagga gcgcaccgtt cccggcctca
     5341 atgtgcgcgc gtcgctgcgg gtgaccttcc gcgatggcgc cgtgcgcgac tggccgggaa
     5401 cctgccacga ggatggcgat cttcccggcc atcccgccgt ctgccgcttc gtgccggccc
     5461 gcaatccgga cacgctggaa ttcggcctgc gcctgaagag gaccggcgcg gccgtcgatg
     5521 ccgtcgcctt cggggacatc ggccggtttc gccgcgcgct ggacgagggt tcggaccggc
     5581 gacgcgacag cgacgacagg gtcttccggt tgcacgccag tacatccgcg acctgtgcct
     5641 tcccggcgaa gtactggtcc gcgaggggcg cgaccagagc gctggaaatt gcgctgccct
     5701 gatggtctaa gccttcggcg gctcgacgat ggcggccacc agtctgcgca ccaccggcat
     5761 cacggcaagc agcgtcggat aggcgaccac gtaggacagc agccaggcct gcatccactt
     5821 gctggcgaaa tccggcgcga ggccgatggc gcgcagcgtg gacacgcagg acacgacgaa
     5881 ggtcatcagc agcgcgagga tcgccggcat gaccagccag gtgtgacggg cggggatctt
     5941 tttcataagc acttactaat gcatctgacg ccaaaagcaa tcccgtaccg cgacctttcg
     6001 cccggccgcg cgcgccccta tatcagggcg gtcctgacag ggagcccgcc catgaccagc
     6061 gtccccagcg ttccgccgca ggccggcgtg ctgcccggcg ccgccctcga tggggtgcgc
     6121 tcgcgccgta tcgtcgcctg gatgatcgac ttcgtcatca tctgcgccct gatgtggctg
     6181 ttcggcctca tcatggtggt gctgtcgctg ccgactttcg gggtttcctt cgcgctggcg
     6241 ccgctgatcg ccttcgtcgg cgtgttctat tccggcctca ccgtctccgg gcgcgggcgg
     6301 ggcacctggg gcatgcgggc gctggacctg caggtgacgg cgcgggacgg cagccgggtc
     6361 gatttcatcg tcgccgccgg ccacgcgctg ttgttctacc tgctgacgcc gttcttcacg
     6421 ctgctgtcgc tgttcaacgc cgagaagcgc acgctgcacg atttcctgac cggcgtgatc
     6481 gtgacgcgcc ggccgcccgg ctgaagcgga cgtgatgcag ccgatccttg cggcgcggcg
     6541 gttggcggct atgatcggtg cgtcagacca gtatcgagac cccggtgacc cagcagccgc
     6601 gcgacacgcc gcagttctac ctgacctcgc cgaccagctg cccctatctg ccggggcggc
     6661 aggagcggaa ggtgttcacg catgtggtgg gcaagcgcgc gccggaactg aatgacctgt
     6721 tgacgcacag cggctttcgc cggagccaga ccatcgccta ccggccggcc tgcgagagtt
     6781 gcagggcctg cgtctcggtg cgcgtgctgg cgcgcgactt cgagccgacg cgcaacatgc
     6841 ggcgcatcct caaggccaac accgatctcg tcgtcgccgt cgtgccgccg aagccgacct
     6901 cggagcagta ttccctgttc cgcgcctatc tcgactcgcg ccatttcgac ggcggcatgg
     6961 ccgacatgag cgtgctcgat tacgccatga tggtggagga cacgcacatc gacacgcgcc
     7021 tcgtcgaata tcggtccacg gcgcgcgaca acgcgctgat cggcgtcgcg ctgacggacc
     7081 ggctcggcga cgggctgtcg atggtctatt ccttctacga tccggacgag gcggagcgct
     7141 ccttcggcac cttcatgatc ctcgaccaca tccgccgcgc ggcggaccag gacctgccct
     7201 acgtctacct cggctattgg gtcgatgggt cgcgtaagat ggactacaag gcgcggttcc
     7261 agccgcagga gcgtctggtt ggcgagggtt ggaagcgggc ttaggccagt ctcgcgacgc
     7321 cgaattccca tttttttcac gcccggacgc gacagcatcc cttgcaacgg cgccgccatg
     7381 cgtcacgatt ggcgaggcag gcgcgctcgc gaggggcaca tgggcagcat tccggacgac
     7441 aacgcgacga cattcaacca cgtcgatccc ggcacgaaac tggcgctctg tctttccggc
     7501 ggcggcctgc gggcgacgtt tttccatttc ggcgtggtca aggcactggc gggcgcggac
     7561 cgtttgaagg atgtctcgga catcatcgcg gtttccggcg gcagcatcct ggctggccac
     7621 atgatcaaga actgggacag ctactgcggg accgctcaga accttgacgc catggaaatg
     7681 gaactgcgcc gcatggcggc gcggggcatg cgcgagcgcg tggtgcggcg ctgggtgacc
     7741 aaaggcgcct ggcacgggct gaaggcgttg ctcggctccg aacaccggcc ctacacgcaa
     7801 agcagggcaa tccagtacga gtacgagcgc gttcttggaa aaggtacggt cgccgattgc
     7861 taccggaacg cgccaaggga aaagcgtccg ccacaggtgc atttcatgtg caccaacttc
     7921 aactccggcg agccctgctg cttcaccagc gacgaattca tcatctaccg gaagccgccc
     7981 ataagcgttg tgaatggcgt cgccaagcgc ccggccggcg cggggcatac cggaacggga
     8041 agcgacaagg acgaacccgt caggaagctc accatggccg tgtcatccga tacggtcgag
     8101 ctttcctatg cggtcgcggc ctccagcgcc tttcccgggc tgttcccgcc gatgaagctc
     8161 aatgccgcac ggctcggtgc ctcggcccgc gactttccgg tgacggtcta tctttccgac
     8221 ggcggcatct acgacaatct cggactggag catttcaagg agctggtgcg ccagagcgaa
     8281 aagaaggcat cgtccgaaac ctcatcccaa gtcagggcaa aacctgacga tgagcggatg
     8341 ctgaaggcca gcacgggcgg cacccatgcg caggcggtgc tggtgtccaa cgccagcggc
     8401 gccttccagt gggacaatcg caatgatttc tatcacctgc tgccacggaa cctgcgcacc
     8461 atcgatatct tgatgaatct gatgatcgag gagcgctggg aaacaacgcc caaggaactg
     8521 gtgcaaggcg agaaggcgcg gaaaattcac ctgctgcggg ccgacatcgg caacgcgcac
     8581 tgggaagccg gagccccacc caagagcgtg cagcagcaga ttcaggacat tcggaccgat
     8641 ctcgaccggt tcaccgacga agagatcacc cttctgatcg atcatggcta tcgcacggcc
     8701 cggcagcaaa ccggcgtcga accggtccag cagtcgtcgc ctttcatggc ggcggcggcc
     8761 ggcgagccgg cgccagtcgg gcggttccgc ctcctcgatg acctggtcaa gacggcagcg
     8821 gagcggcagc ccaggctgtt cgattggcac gatcccttgc gaagcctcgg catgggcatc
     8881 gccttgctgg tgctctttgt cgccgccatc ttgccgtttg tggcgccctt tgcagcctgg
     8941 ggctggtggg atgcgaacga aaagctgttc gattcacgca aggagaacga tgcgaagagc
     9001 gaccgtatcg ccgctcaggc gcaggaactg caaagcatca agaaggcgtt ggaggacatc
     9061 accaacgccc agattgcaag cgccacccgc ctggagcaac tcagcggttc gtctggtgcc
     9121 aaggcacctc cacccgacag ttcaacacct gcggacctgc cagttcttcg gccggatccg
     9181 acacggccaa gtcgcgatgt gctcctgtcg caacgcgaga aactggacga gttgcagaag
     9241 cagatccagt tcaccatcaa gcaagaggtc gcgcggattg agactctttc agcgcaacgc
     9301 gacttcaagc cgcagtcgac acgtgtcgaa gtgaaccgcc agagttacaa ggtctggctc
     9361 cagttcgccg gctcgttcag gcggcaggag atgatcgact tcggcaaggc gctgaaggcg
     9421 aagtggccca atgcgccggg cgccgagcag ggcggcgagc gcacggagaa tgccgcaggg
     9481 ttgttcgaag tgcgctacgg cccgccggag gacaaggcag cggcggaagc actgacgcag
     9541 gacattctcg acctgaagat cgtgccgaag atgaaccagc cgcgcaggct tgcgatcatc
     9601 cccaaaggct cgctggaaat ctggatcagc aagtagatcc tctcaccccc gctccgcctc
     9661 gcgcacgatc atcccatccg ccgggttgac gacgaggtcg cgcaggcggc cctgcctgtc
     9721 acgcgccacg acggcgaaac agcggtcgtc gccggtgcgc agggactgca gcttcaggcc
     9781 ggactcgcgc agtcgcgcct cgacctccag cggcgtcagc cagtggcgcg gtggggtgga
     9841 ggtgcaggtc tgcggccaga gggaaacgcc gaccccggca agggcggcga gggaggcgct
     9901 ggcggcggcg atgagcttca tgacggaccc caggttgaac ggtcccgatc atgatcgata
     9961 caatttaaaa ttgtgttttc ttgtttcttc accctaaagg agatttttcg gtgtgatggc
    10021 tgccggcgtc tcgcgtgcga acgggatcga aacgttcctc ccgagcatcg accgtttcgg
    10081 ccctaaaacc cggggttctc accccgtaaa tcgattgttc ttcgggaagc ctttcggggg
    10141 caggcgaccg gactcggcgc ggtttccttg ccactccgca agctcggccc ctttcaccgt
    10201 ataggtgcgg ctggcgctgt cgagccaggt caatccgtcg gcgagggtga aggtcttggc
    10261 atcggagacg ccgccttcct tgtacttctg caagcggacg cccttgccgc gcgccatttc
    10321 cgggatctgg tccagcggga agaccagcaa cttccggttt tcgccgacga tggcgacggt
    10381 gtcgccggaa accggaaccg acagccaggc ccgcgccgaa tccaccgaaa ggacgttgcg
    10441 tcccttgcgc gtgttggcga cgagatcatc ggaattcgcc acgaaaccgc gcgaatcccg
    10501 ggcgatcagc agacgtttcg cccctggttc atgaggccag accgtgacga tctcagcttc
    10561 gggatcgatg tcggccatca ggcggatggg atcgccgaac ccgcgcccgc ctggcagttt
    10621 cgatgcttcc agcgtgaaga ccttgccgtt ggtggtgagc accagcaact tcgccgtggt
    10681 ctcggtcggg aaggacagct tgacggaatc gtcgcccttg aactgcacat tcgcaagttc
    10741 gacgacatgc cccttgagcg cgcggatcca gcccttctcc gagacgacga cggtgatcgg
    10801 ctccttctcg atcatcgcct cggtgaggtc gatctcggat gtcgcgggcg cttctgcaaa
    10861 ctgggtcctc cgacggccca ggcgggtctc cggaccaaag gacctcagca cgtccttgac
    10921 ctgccaggct accgtcttcc actgcttgga ctcagaccca agcaattgtt ctatttgcgc
    10981 tttttccttt gtcagcgcct cgtgctcgcg cttcagttcc atctcttcga gcttgcgcag
    11041 ggagcgcagg cgggtgtcga ggatggcgtt ggcctgtagc tcggtcaacg tgaaccgtgc
    11101 catcagcgcg gccttggcgt catcctcttc ccggatgatg cggatcacct catcgagatc
    11161 gagatagaca atcagcaatc cgccaaggat ttcaaggcga tgctcgatct tcccaagccg
    11221 gtggcgggac cgtcgcaaca gcacgtcgcg ccggtggtcg agccagcagc gcagggcctc
    11281 ggcaaggccg atgacgcgtg gcgcaagccc gtccaccagc acgttcatgt tcataggaat
    11341 gcgcgactcc agttccgaaa gccggaacag cgattccatc agcatgaccg gatcgacggt
    11401 cttggagcgt ggctccagaa cgatgcggat atcctcggcg gattcgtcgc gaatatcggc
    11461 aagcaacgga agtttcttct cgttcagaag ctcggcgatc ttctcgatca accggccctt
    11521 ttgcacgaga taggggatct ccgtgaccac gacgacccag gttccacgac caagatcctc
    11581 cttctcccac ttggcccgca cccggaaacc gccccggccc gtccggtagg tctccgcgat
    11641 ggaaccgcgc ccctcgacga tgacaccgcc ggtcggaaaa tcgggcccgg gcacgaaccg
    11701 gaggagatcg tccggcgcgg ccccgggatt ttccaggaga tggagcgcgg cctggcaaag
    11761 ttcggcggcg ttgtgaggcg ggatcgacgt ggccatccca accgcgatgc cctgcgcgcc
    11821 gttggcgagc aggttcggaa aggccgcagg caggacgatc ggctcctcat cctcctgatt
    11881 gtaagtcggg cggaagtcga cggcatcttc gtcgatccct tccaggagca gcgcggcgac
    11941 gtcggtcagc cgcgcctcgg tgtaacgata ggctgcggca ttgtcgccat cgatgttgcc
    12001 gaaattgccc tgaccctcga ccagtggata acgctgggcg aaatcctggg cgaggcgcac
    12061 catcgcgtcg tagacggcct gatcgccatg cggatgatat ttgccgatca cgtcgccgac
    12121 gacacgggcg catttcttga aggccgcgcc gggatcaagc ttcaggagcc tcatcgcgtg
    12181 cagcagccgg cgatgcaccg gcttcagacc atcgcgtgcg tccggaaggg ctcggtgcat
    12241 gatggtggac agcgcatagg ccagatagcg ctcctccagc gcctgcttca gctcgacgct
    12301 ctcgacttcg ccatcctttg gcggttcaac cggctttccc atggattttg gctagcaggc
    12361 ccgctgattc ggggaaagtg ccggccgcgt aaggtatcct ttctccccct tcgtcggggt
    12421 tgaccggagc cgatggcatg gccataagcg gccgactgaa aaccggagcc tgccatgccc
    12481 gacaagatga accgcccgaa ggaaattatc gaattctgcc tcaagccgct gaacctcgac
    12541 aagaacagcg acgcctatga ggcggcctat gcgcgtctgg agcatgtcat caagacctac
    12601 caggctcaga tcctgaaggg caagcagtcg gtgacggtcg acttctcgca gatgaagacg
    12661 atcaccatca acgaagacgc gcacggtcac gactgagcgc agctgccgca gagacaaggc
    12721 cccgcccggc tgtgccggag cggggttttt tgtgcccgtt gcctagccgg tcgcgagcgt
    12781 cagcagtgcg gcgcgcgatt ctggcatggc aaggccgcgc ggttgaagca cgtgccgttc
    12841 aaggaagtag ccggtgaggc gaaaggcctc ggccagttga ggggcgcact gctgcgaagc
    12901 cgctcgcggt ttcaggaagc acggcagcgg caggagccgg tcggcatagg gttctccggc
    12961 gactcggctg accgcccggc ccgacttcgg cgacacaaag accaggtcct cgttgctacc
    13021 cgtcgcggcg caggtctcaa gatcgagccc aaagccgaga tcgccgagca gcgccaattc
    13081 gaaccgcgcg atcagcgcgc aggcggtcgc cggctcatcg agatgggcga tgatgacatc
    13141 cagcgcttcg aacagcggcc cgtgcgggtc acgctccggc aacaggcgca aatgctcggc
    13201 aaccgtgttg agcgcataga gcgcggcggc gctgcctaga aagcgcgcgg cgcgaacctg
    13261 cgtgacctcg acggcgtagg tgccgaggtg ctcgtcgagt cgcgcgcgcc aggtcagatc
    13321 cacggcattg cccggctgca acagtggagc ctgccgccgg ccccgcccgc cgcgcaccag
    13381 accgagatgt cggccgtgcg cccgcgtcat ggcttccagg atgacgttgg cctctccgtg
    13441 acgcctgagg ccgataatga tgccgtcgtc gcgccactcc atccgagtca gcctatccct
    13501 tcgggaactc cagccccatc tcgcgatagc gctccggatc gtcggcccag ttctcgcgta
    13561 ccttcacgaa gaggaacagg tgaaccttcg cttccgccgc ctcggcgatc tccttgcggg
    13621 cgagttcgcc aatcttcttg atggtggcgc ccttctcgcc cagcacgatc ttgcgttggc
    13681 tctcgcgctc tacgtagatc gtctgctcga tgcgcacgga tccgtcgcgc agcgtcttcc
    13741 attgctcggt ctcgacagtc gagcgatagg gcaattcgtc gtgcaggcgc tcgaacatct
    13801 tctcgcgcgt gatctccgcc gccaggaagc gcaggggcgc gtccgatatc tggtcttccg
    13861 ggtagagcca tgggccgggc ggaaggcgcc cttccagcca agtcaggatg gtggcgacgc
    13921 cgtccccggt cagagccgac accatgaagg tggcggcgaa cttcgccttc gcgttcaggt
    13981 cagcggcgac ggccagaagc cggtcgcgcg gcatggtgtc gatcttgttg agcacgagga
    14041 acacgggctg acgcaggtcg gcgatccttt ccaggatcgc cagggtctcg tcctcgcccg
    14101 ccttgtggac atcgaccagc aggcccacca tgtcggcgtc acctgccccg ccccaggcgc
    14161 tcgtcaccat ggcgcgatcc agccggcgct tcggcttaaa cagtccaggc gtgtcgacaa
    14221 acacgatctg ggcctgaccg ctcagcgcaa tgccacgcac ctgcgtgcgt gtggtctgca
    14281 ccttgcgcga gacaatcgag actttcgcac cgaccagcgc attgagaagc gtcgacttac
    14341 cggcgttcgg cgcaccgacc agcgccacga agccacagcg ggtaactggc gcggtcacgg
    14401 catgcctcca gcgacaccct cgcgggcaag catcgcctcg gcagcggcac gctccgcgat
    14461 gcgtttcgaa cggccctgac cctcgcaggg ctcgagcccc tcgaccaagg cagcaacgcg
    14521 gaaaaccggc gcgtgatccg ggccggtccg gtcgacctcg gcgtaacgcg gttcgggcag
    14581 cccgcgggta tgcgcccatt cctgcagcgc ggtcttgggg tcgacgccgg cggttgtcat
    14641 gtcgggaaga cgctccagcc agtggcgacg caccacggtg tcgacggttg gcaggccggc
    14701 atcgagatag accgcaccga gcagcgcctc gcaggcgtcg ccaagcaccg caggcttgcg
    14761 ggcaaggccc gacttgcgct cacccttgcc catgcgcaag caggcgccga cgccgatgga
    14821 ttcggcgacg gcagcgcagg tctccttgcg gacgagatcg gccagtgcgc gcgacaactg
    14881 cccctcaggc gcctccggat ggcgggcgta aagcagatcc gccacgcaca ggccgagcac
    14941 gcgatcaccc aggaattcga ggcgctggta actcatcgcc ttcgtcccgg caccggaatg
    15001 gctgacatgg gtcagcgcct gttccagcag gctcttgtcc gtgaacacat gaccaaggcg
    15061 ctcctccaac tcggccagcc gggccgcgtc cgccgccatg tcagcggatg ccccggaaca
    15121 tgcgactcca gcggatggcc cagggccagt tccaaacctc ccagaagcgc acgttctcgt
    15181 cgatggagaa gaagatgatc tcggcccggc cgacgaagtt ctcgaatggc acatagccga
    15241 cctggttgag cacgcggctg tcggtcgagt tgtcgcgatt gtcgcccatc atgaagtagt
    15301 gcccctcggg gacatgatag acgtccgtgt tgtcccagta ggacgtatcg ccttcgagct
    15361 cgatgatctc gtgcgagccc ccgccgggca gggtctcgcg gtagcgcggc acctgcgcgg
    15421 gacgaccgaa cttgtcgttg ccgggatagg tgccgaccag ttcgcgcttc acggcggagc
    15481 cgttgatgtg cagcacaccc tggatcatct gcacccgatc gcccggcagg ccgatgacgc
    15541 gcttgatata gtcggtctgc ccgtccatcg gcagcttgaa gacggcgatg tcgccgcgct
    15601 tgggctcgga cacgaagaag taacggccgt tcgtcggcac cgcgctgtag gggaaagagt
    15661 gcttcgaata gccgtaggaa tacttcgaaa cgaacaggta atcgccgacg agcagcgtcg
    15721 ggatcagcga accggacgga atgttgaacg gctggaacag aaacgtgcgc acgacgagcg
    15781 cgatcagcag cgcctcgatg atgacacgga tggtctcgcc aattccgcct tcgctcttgg
    15841 acttgtcaga cataggctct tcttcgtgaa cgtggatcgt cggcaggagc gtcttggccg
    15901 tgacggccga agcggcggtg gaaggtggcg ctgatataga cgttcgccgg cgtgccagca
    15961 atccgggcat caggccacag gtctggcctc gatgatgacg aaggcctgcg ccaacgggta
    16021 gtcgtcggtg atggtcagat gaatgacggg ctcgtatccg ctcggagtta gcgcatgaag
    16081 gcgctccagg gcttgcccct ccagccgcat ggtgggcttg ccggagggca ggttgacaac
    16141 gcccatgttc ttccagaaca cgcccatgcg gatgccggtt ccgagcgcct tggagcacgc
    16201 ctccttggcg gcgaaacgct tggcatagga cgcggcgcgt tcgctccggc ggtcggattt
    16261 cgccttctcg acctccgtaa agacccggtt gacgaaacgg tccccgaacc ggtcgagcga
    16321 cttctcgatg cgacggatgt cgcacaggtc cgaaccgatg ccgatgatca tcgcgccttg
    16381 cgccccctga gcatcgccgc cttcatctgg cgaattgtgt cgcccaaacc gacgaagatc
    16441 gcttcgccta ccagaaagtg cccaatgttg aactcgacga actcgcgcac atgcgccagt
    16501 ttttcggccg tccgatagtc gagaccgtgg ccggcatgcg gctcgaggcc aagctgggcg
    16561 gcaagacgcg caccctcggc gaggcgctgg tattccctct ccgccttttc cacctcgtga
    16621 tgcacagccg cctcgcacca ggtgccggtg tgcagttcca cgatatgcgc gccgagtcgc
    16681 ttggccgccg cgatctggcc tgcatccggc tcgatgaaca gcgacacccg gatcccggcg
    16741 tgggaaagcc gagcaatcgc catcgccaag tccggtccgg cgcctgcaac gtcgagcccg
    16801 ccctcggtcg tgcgctcctc acgcttctcc ggcacgagac agcaggcgtg tggcttcacc
    16861 cgcagcgcaa tatccagcat ctccaccgtg ttcgccattt cgaagttcag gggcttggcg
    16921 atctccgcct tgagccgaaa cacgtcgtcg tcgcggatgt gtcgccgatc ctcgcgcaga
    16981 tgcgcggtta tgccatccgc gccggcctct atggcggcaa gggcggcgcg gaccggatcc
    17041 ggcacggctc cgccgcgcgc attgcgcacg gtggcaacgt gatcgatgtt gacgccgagg
    17101 cggatgggcg taccggacat gtcgaaacct ctgctgcgaa ctcgggcgaa acctcaaaag
    17161 cgaaggccgt ccttcatagg ctatcgcctt agcctaatgc ggctttccct aggtttgaac
    17221 cacttcgcgc gcccgtgcca gagaaccgat gggtcagcac atcatttttt tgcgcagatc
    17281 gtctttagcg cggcccattc ggcttcgttc agcaacggcg ggccatcaag cggacgcccc
    17341 agactggcat cggccgcctc aaggcgcttc agccgttccg ccgtcagcgg gtgactgtcg
    17401 aagatactgc ccttcgcgcc gggcttcgaa gggtcgatcc gggtcaaaac ccgggccagc
    17461 ggcgcagcgg gccggcccag cgcatgcatc aggtcgcgcc cgaagatgtc ggccgcagcc
    17521 tcagcctcgc gcgaatgggc tgaactgatg atttcgcgca cgaccagaac gagcgttgcc
    17581 gagccggtca ggtcaccgaa cagcagcccg agcaggaagg aggtgccgcc tgcctcgatc
    17641 agagcgcgca agccgtcgcg gttcgcctca tggccgatct cgtgcccgag cacgccggca
    17701 acctcgtcag gatgttcggc gcgggcgagc aggccatcaa acaggaacac ccgcccgccg
    17761 ggaagtgcaa cggcattggg aatgcgcgag ctgagcacct cgatccgcac gggatgaggc
    17821 aacctggctt tgacggtgag cttttccgaa agcttcgcca gggcggcacc gccctcgccg
    17881 ccgacgcaga cttcgccgcc gaacatcacc ttgacctggc cggccacggc atcgccaagc
    17941 ttgcgctccg cccagaccgg tgtgatcgac gcaacggccc gcgccatctt gggcacaccg
    18001 aaaaaggcga cccccaggat cgacaccgcc gccgccagcg acagggcaac gatgcgcaag
    18061 atgccgtgcc taggttcttg cccatcgtag cgcagacgag cggcccgtac ccgaatggcc
    18121 tcgatcgtgg tgggatcggc gatcgtcagg ctggcaagtt ccggcgcggc aaccgagcgc
    18181 accttcagcg tatcaggcaa cgaatcgagt tgacgcaggt ccaggaagct ccactcagcc
    18241 aggcaaacat cgccatcgat gatttccaga cctgcatcga tgcgcacggt gacatcgcgc
    18301 cgccggctgg aaacgccatc gaagtatgcg ccggggcagt gctggtccat caaaacccgt
    18361 ccgatccgag cgcatcggcg agaccctcgc cgagtgagcc ttccgcatgc cccttctgca
    18421 ccaccgtctc gagcgcttcc gcattggtca gcgacgtgct cgcagcgaag cgctgcatgc
    18481 ggggaaaatc gaagaacagg gacttcagga atgtgatgcc gacaaggccg atcagccccc
    18541 aggcgaagcc aagcagcagc cagggcgcgt gcctcaccag cgcgccgaga accgcggcgt
    18601 cggatccttg cggcacctcg aagctcgcaa gtcccaaaag tgaaccccag aacccgacat
    18661 agccgatgcc gatccagact gctccgaaca gcccgacggc cagcgccgtg accacggccg
    18721 gccccagcag caagaagcta tcgatcgacg attgaaaccg caccgggcca aaggacacgc
    18781 cgccaataag ccaatggcca taaacggcga tgatcgctga catcatgatg ggaatggcaa
    18841 gaaggttggc aatgggaatg aacgcgccga caaccagccc caaagccggc cagaacaacg
    18901 ccttgaacag cccccagccg gttccgtgga aatcgccttg aaggtcgccg aaatggctgt
    18961 gacgccattt gaaccgttcg agacccgccg ccatccaggg ccaagcgata ccaagggtca
    19021 cgccggtcgc caccatccat cccaaggcag tgaaggcgaa ccgccaggcg ctgccgtcca
    19081 tccagaagcg cacgcctcgg aacacggtcc gcgtcaaccg atagcgtcgc gcccgataag
    19141 ccgcgaaata gctgaatacg atggtgaaaa gggcaaaggg aaccgaggcg aaagcctgga
    19201 ccgtctcggc gtagaggccg aggatgaagt aggtcaccga gatcggcaga tagacggcta
    19261 tggcgaacag gaatccgacg agcaactcgc gcgccgtgcc atggtaggcc agcgcctcgt
    19321 cgttcaccga gatgctgcgc cacaggaagc gccgctgttc cgtcttcagc cagaagcggt
    19381 agaagccgaa cgtcggcaga tacagcgcca tgccacgcgc catgatgccg acccagtcgc
    19441 ggcggctccc gtggaaaatg acgggtgtta cctcctcccg ggatggcgct gggtcggctg
    19501 aggtggcggg cttggtctga ggcgcagact ccgtcggcgg cggcggcttc aggtcggcct
    19561 ttcgcggtcc ccagggaccc tccggatccg attctgaagt catgatgtcg cctccgctcg
    19621 cggattcgct ccgatcttag cggctctccc gcagagtcga tacctgtccg accgatcctg
    19681 ccgcagcttg cgcgatgccc tctcccggcg acaggagagg gcgaggcgtt ggcatgccta
    19741 gcgcgacatg agcacgggta tgttcgcggc gcgcaacagg tcttccgtga cacccccgag
    19801 gatcaattcg cgccagcggt tgtgtccgaa cgcgcccatg accagcaggt ccgccctcac
    19861 gaggccgaca tgagccttga tcgtgttggc gaccgtgtct cccttgcaca tgaggtcggt
    19921 gacggtgacg tcaatgccgt gccgggccag cgcaggcgcg agttccgcac cagggaccga
    19981 cttcgacagg tccttctcat ccttgatcga gacgatctcg actttctggg cctgtttaag
    20041 cagcggcaat gcatcccaga ccgcgcgtcc ggcccgggcg ctcccgtccc acgcgacgac
    20101 gatccgctcg aatgccgccg ggcgcgcgtg gtcgctgtcg accagcatga ccggacggcc
    20161 actctcgaag atgaggctct cgatcaggcc gggcatgaca taatcgtcgg cgccggggcg
    20221 gccgaccagc accagttcgt ggatatgcgc ccggttcagc aagcggcgct ggagagacgg
    20281 gaacggggac tgcagcgtct ccgttgacat cgtaatgccg ctcgcaacag cgctccgtcc
    20341 gagcgcttcg gacatggtgg cggcgttggc cgcgcgcacc cggtcattgt cagcgagcag
    20401 gccggtcacg acgggaacgg gcagaaatac gctttgctgc agcttcggag ccgccatcac
    20461 caccgacagg tgggcgccga gcttgctcgc caaactgatg ccataggcgg aacaggcagg
    20521 gggatgcacg ttttccgcga acggcgcgtg caaacacatc aggatcgact tcatgacttt
    20581 cctccaggat acggagatca gttagggtca acctgaagga tttccatgac gcggatcaac
    20641 gcgccgagcg atcgctatcc gccgaccgga gccggagcgc cgcgcgccgc cgctgcggcc
    20701 agttcgggca gaacgccccc ctgatcaagc gacttgacgg ccaccgtggc ggaggcgaac
    20761 tcgatgccct tctccgggaa gacctgcaac atgcgcttga ccgcgatacg ctggatgtag
    20821 gacgggcgtc ccggctttac cgtgaacttg aaccgggcga tcagtgcatt gtcctggatg
    20881 tcggcgatac cttgcatctt caggggcagc aggaaatcgt ccttgagatc ggggtcctcc
    20941 agcagttcct gcccgatctt tttcgtggcc ttgcgcaacg cctcaatgtc ggtttcgcgc
    21001 gcaaagcgca ggttgaactt catcgtggcc cagtcgcgcg agaagttggt gatctgcccg
    21061 agctggccga aggggatggt atggatctgc ccgccctggt ggcgcaggcg gatagaacgc
    21121 agcgtgaagc tttccaccgt gcccttggcc ttgccgatgt caatgtactc tccgacgcgg
    21181 aaggcatcat ccgccaggaa gaaaatgccg gagacgatgt cgcgcaccag agactggctg
    21241 ccgaaggaaa gggccaggcc gaaaatcgag gctcctgcca gcagcggcgt aacgttcact
    21301 ccgagctgcg acaggacaat gaggacggtg aagccgacga tcagcacgcc gagcacgctc
    21361 ctgagtaccg gcatcagggt ctgcaatcgc gatgcgcctt ccagcgtgtc ctcctcggtc
    21421 tcgttctcgg ctcccggaat ggcgggagca cgtgccggcc cggcaacctt ctcgaaatag
    21481 aacttggtca cctgccagat cgcccaggcc gcgaagacca cgaatccggc acccgccaga
    21541 gtcgcggagt acccggccca tgccgaatgc gggatgaggt tcaggatttc cgtgacaacg
    21601 atccgcaccg cgatgaggaa gatggcaatc cgcaccagaa tgttgagtcc acgccaggcg
    21661 atatcctcga gctgcggggc ggtgatgccg gcggacttct tcgcgtccag cctgcgccgg
    21721 tggatgcaga acagtatagt ttcgaccagc agccagccga tgagcaactg ctggagatcg
    21781 ttgagcgaag ccggcacatc gacgcggccg gacatgccgc cataagcctg cgcggcccag
    21841 accagcacat agaacagcac accggggaca tgccagttgt cagccagcac ccggcgccat
    21901 gaacgctcgt cgttgagcga gccgagccgc gcgagccagc gtgccatcgg catgcgcacg
    21961 gcaagaacca tggcgatgaa cgtaccgacc acaagcggcg caaacaggaa ctggtacaac
    22021 acgatctcat cgcgctgcgc attcgtcgcg acaagaatgc ccgcgaaggc gcgcgtgatc
    22081 gacggcactg cggtcgccat ggcaaagccg cgcatcaaca cggccgcgtc atggtcgtca
    22141 accggcgcaa gccggccggc cggcagatgc ggtcgtagcc agcaccgagc aacggccaga
    22201 ccgacgcgcc accagaacag ccccatcagc acgagcccgg cgatgcggcc atgctcgccg
    22261 ccggaaagaa acagcgacgc gaagacagcg ttgccgacca cagcgaaggc gacgactgca
    22321 ccagcttcca ccgccgtaag agcaaaccag cgcgcccacg gcggcggcga ttccacagac
    22381 cgcacgatcc gcttcacaag cccagacgac gccatgcgcg cggcccattc ggccgccaac
    22441 gcgagacccg ccacggcggc gattttgatc agcaacccaa tgaacccggc atccttcggt
    22501 ccggacgcgt caagccgggc cggcaatccc gcgacatgcc ggacaaggcc gggaaactgc
    22561 gatgcgatca cgcccagtcg cgacacaagc tgatggatgg tttcggacgt ctcctccgca
    22621 ttcttgcgaa cggcggcagc ctgcgcggag gccattccct ccccggcaag tttcttggct
    22681 accgcatcgg cgaccgaatc gacaaaccgg tccatctcct gtttcgacca gcctggcggc
    22741 ggcgtctgcg ccgaaacggg cgcgatccat gcgcaaaaag caaagacaaa tgacagcgaa
    22801 aatgcccgta acgcccgcgc aaacatgccg gtctcccatc cagtccctgc aaagaagcat
    22861 ccacaacggc tgccggcaac tccctgtttc ccgaaccccg ggtttcaggg catagaactg
    22921 gctcgtcaaa aggacttgca atgcgcgaac cagccccgag cctcgactcc ctcccctttc
    22981 gcacgcagca tgagcttcgc tattccgaca tggacgaaca ggggcacatc aacaatggcg
    23041 tatcctcagc gctgatggaa cattgtcgcg tttccctgct gcgcggcggc ggattgaagg
    23101 cgcgcttcaa caacaccttc gtgctcgctc gcaatgagat cgactacttc gccgagttgt
    23161 tctggcccgg tcaggtgacg gcggcgatcg gcgtagagcg ccttggcgag acttccatca
    23221 ccttccggca ggccattttc cgtgatggtt cctgcatttc ggcgggtcgt gcagtggtcg
    23281 tcgccttcga cgggcagacg cgccgcccga caccgcttgc catggaagca cgccgagaac
    23341 tcgaagcctg gcgcgtacga tagatctggc gcctgtcttc gccgaccggt catccggacg
    23401 gcaatccgcc tcgggcgacc aaggcgaagg cataaagtcg ggttggaaag aacggaccct
    23461 ttcgcgcgcc cggcgtttga ctatgatctc ggccatttct tagagagcga agaatcggcg
    23521 cggccgatct cgccgccgga gttgccatgc catcccttgc cgcaccaccc gccccaccgc
    23581 gctggaaggt ggtgctgatc ctcatgctca tcgccaccac attcggtagc aaccacgtcg
    23641 ccgcacgctt tgctttcgat cacggggcaa gcgttttcac cgctgccctc gtgcgttcgc
    23701 tgggcacagc actggctctt gtggttgcac tgaaggcaac cggcgtatcg ttgaaggttc
    23761 cgcgtcatca ggttttccgc gcggccgtta ccggattgct ggtcgcctcg gccagcgtgt
    23821 tcatctattc cgccgtggcg cgcataccgg tcgggctggc gctgctgacc ttcaacacgt
    23881 tcccgttcgt cttcacgctg gtgacatggc tgatggacgg aaggcgtccc tccggacgaa
    23941 cgctcggctt catggggctg gcgcttgcgg ggctgtcgct ggcgctgacc tttggcggag
    24001 gcgccagcta tccgccgcca gccgtcatgg cgacgggcgt cgctctggcg ttcgccgcct
    24061 cgctgtcctt cggcggcgtg ctctacctct cgcaacgctg gctcggcgcc atggacgggc
    24121 gcgtgcgcac gttgatcatg atgtcgacga cggccatcgt cgtcggcgtg atcggaaccc
    24181 ttggcgacgc ctttcgcctg ccggtcgact ccgtaggctg gaccggcctg gcgcttctaa
    24241 cgatccttta cggctccgcc ttcaccgcgc tgttcgtggt gctgccgcgc tacggcatgc
    24301 tcgacaatgc cggcgtgctc aacttcgagc ccgtcgcggt gctcgcgatg ggcgccgtcc
    24361 tccttggtca gacgatcacg ccggtgcaaa tgctcggcgc cggaatcgtg ctggtcgcca
    24421 tgctgggctt cgccagcgag gggaagcggt agggggctgc cctcacatcg ccagcgtgta
    24481 ggccgtcttc accgtggtgt agaattcgcg ggcataggaa ccctgctcac gagggccgta
    24541 actcgacctt ttccggccgc cgaagggcac gtggtaatcg acgcctgccg tcggcacatt
    24601 gaccatcacc atgcccgctt ccgcattgcg cttgaaatgc gtggcgtact tcaggctgct
    24661 tgtgcagatg ccggaggaga ggccgaactc cgtgtcgttg gcgagcgcca gcgcctcgtc
    24721 atagtcccgg gctggaatga cggacgcgac cgggccgaaa atctcctcgc gcgagatgcg
    24781 catggcgttg gagcagtccg tgaacagcgc cggctggaga tagtaaccgg gcgtatcccg
    24841 gttcaatcgt tcgccgcccc aggcgagttt cgcgccctca tcgcgaccga cggcgatgta
    24901 cttctcatcc tgatcgagct ggctctggtc aacaaccggg ccgatatgcg tgccggcctt
    24961 cagggcatca tccacgacga ggcctttcat gcgctcgacc atggctgcga cgaatgcatt
    25021 gtagatgccc ttggtcacta tgaggcgcga ggaggcggtg cagcgctggc ctgtggagaa
    25081 aaaggcaccg ttgaccgcgc attccaccgc cgtcttcagg tccgcatcgt cgagcacgac
    25141 gagcgggttc ttgccgccca tctctagctg aactttcttc atcggctgcg aggcgacgca
    25201 gctggcggcg atgcgccggc cggtcgagac cgaaccggtg aaggagattg cggccacatc
    25261 ggcatgatcg atgatcgcct gcccgaccac tgacccacgg cccatgacga ggttcagcac
    25321 gcctttcggc agtccggaac ggatcaggat gtcgcagagc gcccaggcgg aatgcggcac
    25381 gagatcggcg ggcttgagca cgactgtgtt cccccaggcc agcgccggtg cgatcttcca
    25441 ggacggaatg gcgatgggga aattccacgg cgtgatcatg ccgaccaccc cgaccgcctc
    25501 gcgcgtcatc tcgacgccaa gaccaggacg gacggagggc actgtctcgc cgccaatgcg
    25561 cagcgcctcg ccggcaaaga aggcaaggat ctggccggcg cgggtcgcct cgccgatccc
    25621 ttcggggagt gtctttccct cctcccgcga caggatgcga ccaagctcct cgcggcgggc
    25681 gagcacttca tcggaggcct tcttgaggac gtcatagcgc tcctgcggcg tcgtacggct
    25741 ccaggccggg aaggcggcct tcgcggcggc aattgccgcc tcgacatcgg cacgcgatcc
    25801 ggcggcaaac tcgccgatca catcgttggt gtcggaagga ttgacgttgc gcgacgtggc
    25861 cggggatgaa accgtttcac caccaatgaa attggggaaa atcgacacgg gaaccgctcc
    25921 tgactgacaa tacgcccaag gtgggcaatc tggtccaatg cgagatcagg tccaccacgc
    25981 aaccgaaccc gcgaactggc tgacctgcgg cgcgatctct cgccggagct tccagatagc
    26041 gcggcacagg tcatcataca accggcacgt cgtgctctgc cttttccgga gatccggcca
    26101 gggcggctcc gtcttgcacg gcgggtgtca cgacccgccg aacaccggga cgctgcgcac
    26161 atctgtcagt tgtcgaccgt ctgcacgctg gtcgccacct cgatttcctg aagcactcgg
    26221 tccatagtcc tgttgatgcg taggcgtccc cgtcgttttt catatggtca ggccaatcca
    26281 tgcggatgat atgccgagtg gcacagctgc tatcgttgat cgtgctgatc gtcgttgtgt
    26341 ttggcatcgt gaatttcgtg catttccgcc ttcttcctgt gcgggtcgta ctctacgatg
    26401 ccgttctcga tctggtcgtc gccttcgtca tctgcctgct tctgtacggc ctgttccggc
    26461 gttcatgggg cagtctgagc ggcatggaag ccatgctggc gatggcggtc ggatttctga
    26521 tcggcctgtt ctatgccatc tccattccca ccgtcgtcga tcgctcattg tccatctaca
    26581 tcctcgaaaa agtggcccag cgcggcggcg cgatccgtga ggatgcgctt ggagacattt
    26641 ttatcaacga gtacatgccg gaacatcggc tggtcgatat ccggctcacg gagcaattga
    26701 attcagccac gatcacggtc gaaaatggct gcatccggct gaccgagcgc gggcgccgga
    26761 tcgccgagat gacccggtgg tatcgcgccc atatccttcc gaggcatcgg gaactgcgcg
    26821 gggaactgac cgatgcgctg accgatccgt tcaggcattc caagacggac gttacctacc
    26881 agtgccgcta ggcggcatgc ctttggaaag ccccgcagag ggccggtagg gcttgttcca
    26941 ggccacaaag gcctgtggca gacgtctctt tccggcctgg agtcaggcgc gatcaacgcg
    27001 ttcgatgagt tgatcgagcg tcgcctgcga ggcaggcggc gcattccgga tgaaccccga
    27061 gaccatcaac gtgacaaagg tcaggatacc ggcctgcatg acgatcagca cgagcacgcc
    27121 cacagcgatc gagaaccagc ccggcgtggc aaatccgatc gtcttcagga cgacggtcgc
    27181 cacgagcgat ccgagcgcga gagcggaaag aagaatgcag gagatgccca ctcgcaccag
    27241 aacgtcctcg gcaaacacga tgattgagcg cagaccgtgc aaggccagcg aggtgaagtt
    27301 catgcgggac cggccctcat agcgcgtacc gcgatcggtc gccaccgaac cgatccgcag
    27361 ccgcgacacc atgacggccg cggcgacatg cacccaggtc tcctgcatcg cggcgagcct
    27421 ggccaccgcc ctcatggaaa gggccgagaa gttgccaaag cggatggagc gtccggtcag
    27481 cagcctgaaa agctggcgat agacaaaata gaacgtccga aacgaccaag gttcggaacg
    27541 cctgcgacgt tccgcgacga caatgtcata gcggccttca ttcagtgtcg tgagcaaggg
    27601 atcgatcgag tgcggctggt cctctccgtc cgaatccatc acgatgacgg catccgcgct
    27661 gaagttcgct gcgacataga cgataccgac cgctatcgcc cgctgatgcc ccatgttccg
    27721 cctgagatac actacctccc cggccagccc tgccgcttgg atcgcctcac agccgagcgg
    27781 atcgcgggtc gaaccgtcgt cgatggcgac gacatagggt gccacagcac attccctcgc
    27841 caggtcgcgc agcagatgcg tcgcactcgc ccgatcttcg tagttcggca tgatcacgat
    27901 cagcgcgtgg tgcggctttc ggcctgtatt tgcttcgttg accatcgctc gaacccttgc
    27961 ttctgacggt tggagtcttt tcgcccggag cgcggcgagg gggaatgacc gagcgcaatt
    28021 gcgggtgacg cgcgacctag gggatcttgg cgaaggtgta tccgtgcacc gacaacacat
    28081 cctgagaccc cacgcacagg cggatctcca ggtcgctgat ttcctcggca agcaagactt
    28141 cgcggtcgag cagccgaccc tcctcacccg gaccgttcag cgcaaaggca acatgctgca
    28201 cccggccggt gcgggacgtg acgtcgacga cagcctcggc cagcctggtc gcacggccct
    28261 cgaccacaag tctgtaacga cccgcccaag cccgcgtgta gggaccgaag aaaagacatc
    28321 cggccgttcc atttgtttcg aggtgggtgc ccgtgaaagc gccggacttc gagggcaggc
    28381 taccgagctc agccccccgg taggaaatgg ccgtcgccag cgcgaaggca agtacgggag
    28441 cagtccccga cggagcgccg gcggaagcct gaacgacatc gaccggacca accactgccg
    28501 atacctggcg tcggcactcg gcctgagcag cttccagaca gacaaccgga aaagcgccgg
    28561 cgcttgccgc cattgccgcg acggccgcgc gtgcagcggc accgttgccc tcggcgcggt
    28621 acccgagccc gtatgcactc ctgtcggcca gaaggtcgcg catcaccgct ggccaggcca
    28681 cccaatagtc accagcgtaa agcccgaccg gttgcggcac cgccacgttc acctttcgga
    28741 agacggcata gtccgagaag gcgaccggcg acctgaccaa ggctgcaatg gcaaaggcca
    28801 aagctgcgaa agtcaccacc tcccacctgc ccgagcgccc caccgtcagg gcaccggcga
    28861 taccgaaggc gcaaagcaga acgagaccat aggtcgcgaa gacaaaatag cgggaggcgt
    28921 gtccattcgt ggccacccac tcattcgacg aaaacaggac aatccagccg atgctgaagg
    28981 cgacgaccgc ggcgaacaca aaagcaatcc tgccgttcat ctctgcctgc ctgccccagt
    29041 gccggaacaa ggacagcgcc ccaaaaacaa tgatgaccgc aacgaacgcc gcgccagcag
    29101 ggtcaaagtc caatctgagg ttggacaggg tcggaaggat gccatcacga aagccctgaa
    29161 ggctgaatcc ggcatagcgc gactggccgt gagccgcaga gagcaggctc caggcgtagg
    29221 ccgaagccat cgtcatcccg acaaccgcaa tggcacgcag gcgaaagcgc gcggccatga
    29281 gcgcaacaat tcctgcggca tacgtcatcg ggatcacggc cgcgaagtta atgccgatgg
    29341 ccacaaagat gcagagcgcg gccagtgccc agcgaatcga acgcagcgct cccgcttgaa
    29401 ccgccagaat tagagcaatg ccaatcaaag ccgcggccat cgaatactcg ggatgcgcga
    29461 tgatcagcgc ataggacacc gactcctcga tcaggagcaa ggtcgccagg cacaggactg
    29521 cgaagatctg aaactcagcg agcctaagat gccctccgag cctgaggaat tgggacacgc
    29581 cccgggcgat gagaaagagg agcacgaaat gcgctgccgt cgcgagcacc atgagcgcaa
    29641 agaggttcgc cgccggatca cggataaatg agactgccag cggcaggaca ttcgccagtc
    29701 tgttctggcc ccaatagaac agcgtcacgt tctgcaacga catgatcgac atgaggatca
    29761 ggtcagcatc aagcttctcc ggagcaaatc tgttgacgaa caggacagcc agaatcgccg
    29821 aaatgcccag cgccaggctg atcttccgga cttcccgccc gcccttcggc aagtccggcc
    29881 cggcgtcgtc cggttgacgg ccggcatcgg gaaaagcgcg cctcaagagc gccagcacgg
    29941 gaaacttcac caagcccacc aaccgcaagg taccgaaggc ctcgcgccaa gccaacgaat
    30001 ccgccacgcc gcaggtgcat cgaagaacca tcgacccggg cagccactag actagttgca
    30061 gccataatgg gcttcaagat gccccagagc cggtgcggca tttcgacatc cgatgcgagc
    30121 accaagctgc ttggttgttg cccgatgctc cagactggtc gccacggacg caagcgggcg
    30181 agccgtccga cgtgatgctt gccgcacgca ctcgaacgcg cgcgttggtc cgccaaaggc
    30241 attccggaga ccttcaacag ccggcttggg agaatgcccg gccggcgcac cggcgcacgg
    30301 tcccgccttc tctttactgg cgccagcaag agcaggtgga cgttcgcgaa ggcagtcgcg
    30361 cccaaatgcc gcttgctctt ccaggaaagt gaaattcgat ggagaatccc ggagggagag
    30421 atggtgccca ggggcggaat cgaaccaccg acactgcgat tttcagtcgc atgctctacc
    30481 aactgagcta cctgggcgga agctccgacc gggcgcgccc ggcgagagga agcccgcgta
    30541 tagtcgctcg cttcggccct gtccagaggg tgcgaacggc aaagatcacc ggctcgctat
    30601 ctctcttcgc cgcgctccgg caggccttca tcttcctctt ccgcggggat cgcataggca
    30661 ccggcaagcc accggccgag atcgacgtcg gcgcaccgtg cgctgcagaa cggacgatag
    30721 cgatcgaccg ggcgcttgcc acagaccgga caggggcggg cattgtcgtt cgccgggaag
    30781 ggagcggggt cttccgacat ggtcagaccg aattcgccca actgaaatgc agtgggaaac
    30841 catcgccgac cagcaggccc gccgtctcgt agagcgggag accgacaaca gcagagtagg
    30901 atccaacgag tttgaccacg aaggcgccgg caagcccctg aatggcatag ccgccagcct
    30961 tgccgcgcca ttcgccggag ccgagatagg cttcgatttc cgattccgag agccgcttga
    31021 accgcacgcg cgcttcggca atgcgctgac ggaacgcatc gccgggcgtc acgaccacga
    31081 ccgaggtgta aacccggtgc gaccggccgg acaacagacg caggcaatgg gccgcttcgt
    31141 caaccgtatg cgtctggggc aggatgcgac tgccaacgct gacgctggtg tccgccgcga
    31201 cgagaaatgc ccccgccagc gcgggatcgg aacggacaac cttaagcgcg gccttcgcct
    31261 tttctcgcga aagcctggtg acgagcgccc gaggtttttc gccgcgctgc ggggtttcat
    31321 caattgcggc ggggactaca gcatcgggca cgacgccgat ctgctgcaac agcgcaaagc
    31381 gtcgcggaga tgccgacgcc agcgccaggc gcggtcgcca gccgtcggtc accgcagtca
    31441 tgcctgtccc gccgccgaca gagagaacat gacgcttact tgaagcggta ggtgatgcgg
    31501 cccttcgtca ggtcataggg cgtcatttcg cagaggacct tgtcgccggc cagcacgcgg
    31561 atgcggttct tgcgcatctt gcccgctgtg tgggcaatga tctcgtggtc attttcgagt
    31621 ttcacgcgga acgtggcgtt cggcagtagc tcgatgacga taccgggaaa ttcgagaagt
    31681 tcttccttgg acaagggggg cgctcgcagc tgaattcggg gaagctcgcc agcggcttaa
    31741 ccgaaaccgc agcgaatgtg aacaggctat gcgccgcccg tcccgtttcc gagcgaaccc
    31801 gcacccgcag gtttgcggac gggcacgatg gccgttcagc gacgatttag gaattgatgt
    31861 ccataaacac gggatgcttc ctgcagctct ccgccctatt tcgcttcaag aaatgcgttc
    31921 gggcggctat atcgcgcgaa aacaacactt gcatcgccgt caaaggcgac ctgcggaaac
    31981 cgccgaagga aattgaatgt ctcccttgcc cccgatcacg ttcgacgtcg ccatcgtcgg
    32041 cggcggcccc ggtgccctga tggccgcgat gcacatcaat gaaaaagttg gggccgaggc
    32101 gaaggtcacc atcttcgaac agtccaatcg tctcggcggc aagctgatga cgcggtcgtt
    32161 cgacaaggtg ccggcgctgt atgaggctgg cgtcgccgaa atctacgatt acgcccatat
    32221 cggcccggac ccgctgaaag agctgatcac gggcaaactc ggccttgatg tcgtgcggat
    32281 ggacagcgaa tgcgtcgtca ttggcgacaa gattatcaac acctacgcgg agatcgagag
    32341 acatctgggg aagaaggcat ctgacgcgat tgccaagttt cggaagaaat gcaccactat
    32401 tctttcacct cagcaatact atgagggcat aggacgcgac gacaacgacc aggaatggct
    32461 gtggaagagc ggccttgacg ttctccacga cgaggtaaag gacgagacgg cacgcaatta
    32521 cattcgctat aacgcacaca gcgacatcgc catcgcgccg aatgaaactt caggacttaa
    32581 tgcctgcaag aatttcctga tggactggga tgaatacata aaatattata gcgtcgtcgg
    32641 cggcaacgag cggatcacgc aacgactttc ggagatcgtg agggccgatg tcgagatgca
    32701 cagccgggta cggcgcgtcg gcaaggcgcc aaacggcaaa tatcgcctgg tcgtgcagca
    32761 aaacgggaag gaggtcgaac gcctgttcga cttcgtcgtc gtgtcactgc cctacaactg
    32821 gctggcaacg atggagtggg aaggggagcg gctctatcgg gctctgggag cgcatcttga
    32881 ccatttcaac taccccgccc actacctgcg tgtcgccatc ctgttcgaga agccgttctg
    32941 gaaggatttc gtgcagggtt cctggttcat gtcagacgcc ttcaacggct gctgcgtgta
    33001 cgatgagagc tccaggcatg actacggcaa ggccggggcg ctcggctggc tgatcgcggg
    33061 atcggacgcg ctggcctacg ccaacatgcc ggatgaccat ctggtgcgca tggcgctcgc
    33121 gagcctgccg ccgcaattgc agttcgggcg cgaactgatg ctcgaggcga aggtcgtgcg
    33181 ctggctcaat accgtgaacg gtattcccgg cgggcgtccc gtgcgcgaca cgcagcagaa
    33241 ccaccagccg gagccggtcg agcaccccgg ccttttcgtc accggcgatt atctgttcga
    33301 tgcgacgctc aacggcgtgc tcgacagcgc cgactgtgct tccgacatgc tgatgtcgga
    33361 atacgtgaag gcggactacg aggcgcgcat ccggcttcgc acccgaggcg ccgaagccgc
    33421 caagcgcacg gcagttgcca gttcgcgcaa tgtcaatcga gcgtatttcg agaactactg
    33481 gaaccacgga ccctacccgc aggcctggaa caagttcttc catgccggga tcctaaggga
    33541 ttctatccga gacgtgtggg gagcaggcaa gaccttctcc atgctcgatg ccggatctgc
    33601 cagtggcctg accgtgcagg cgctccgcaa ccttggcctt gatgcgtggg gaattgaaaa
    33661 gaacgcatgg attcacgcac agacgccgaa ggatgtgcgc gcctacaaca agctcggcga
    33721 cgtgacggaa ctgcctttca aggatggaca gttcgacttc gtctacgaat cctgcctggc
    33781 caacctgccc cggcacaagg ttcagaaggc gatccgggaa ttgcaccgcg tcgcccgcaa
    33841 gggcatcatc ctggggtcag tgacctccga cctgacctgg ccagtcctga tcgacaacga
    33901 aatgcagcgc ggcgtgaaga cctttgccac actctggcaa tggtccgact ggctgttcaa
    33961 cgtcggattt gaccacgcca tcgaggatga tgcgctgctc aagcgcatct gggatcggca
    34021 tgatgccgtc gggatgggca agggcggctg gttcgaagat ccggaaagca tccgatactg
    34081 ctatttcaac cggcttgatg tgtgaaagcc gcctgccacc ctaagaaatg aatcgctttt
    34141 cacttccggg gatttcgggc taggacaggg cagggctgcg ctgcctcgac ggcgcggcga
    34201 accgttgtgc cggctcgccg gcctgtctgg aatgccacta tg
//
LOCUS       DAOMXN010000011        30127 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_1012_length_30127_cov_11.782987, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000011 DAOMXN010000000
VERSION     DAOMXN010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 30127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30127
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1012_length_30127_cov_11.782987"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..1109)
                     /locus_tag="PK812_02320"
     CDS             complement(30..1109)
                     /locus_tag="PK812_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HRJ68415.1"
                     /translation="MKIAVIGGGHGCYAAAADLSEAGHEVRFWRQDAAAFAPVLATGT
                     VRLKDFRGSRDVRIAQPTLDIGQAVHGADLILSPLPAVAQADIARRIAPHLADGQVIF
                     IPPGTLGSVLMLQVIRAAGSTADIAIGEAGTLPWLTRKHGPAEIAVTTRASRLPTGIF
                     PARLSDAALAVIRRAFPACIEPVEDALSAALMNAGPIIHPPLILMNAGPIEHFERWDI
                     HKEGTQPSIRRVHDALDGERIALREALGYRPPHFPLADHYATANWMYGNLAHDKLVGS
                     GDWHETLNLSTHRYMAEDVALGLALLVSIARWAGVPAPTAEGLLQIGGVAAGVDFLRS
                     GRTLATLGLDGLDRAQMADLMAGGI"
     gene            complement(1136..2587)
                     /locus_tag="PK812_02325"
     CDS             complement(1136..2587)
                     /locus_tag="PK812_02325"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HRJ68416.1"
                     /translation="MWKPPEKIKHETRPGPWPTAAEAAGSRLFSPVRHRALALESRTW
                     VPAMVPWRATEDGFVTQDNLDWYARFAQGRPGAIVVEATGIRDVPSGPLLRIGHERFI
                     PGLKRLVEAVREASGGHTRLFIQIIDFLNIRRRPDPAKFLERFLTISARHRDALGARD
                     WPEADVRKALASMPLAELKAILTDREYEDLTIGFRERVTDTHLPHIAELPVVLPGLFA
                     NAATRAREAGFDGVELHYAHAYTMASFLSRTNSRSDGYGGALENRLRLPLEVYAATRK
                     AVGDDYVLGCRYLADECIEGGNTVEDATGIGIAFARAGMDFLSLSRGGKFDDAKQPKV
                     GESAYPYTGPSGYECMPGYISDAFGPFGRNVEPVGRVRAAIRATGLETPVVAAGGVHG
                     FAQAEAILADGKADIVGLARQALADPDWFRKVRSGHGGAVRLCEYTNYCEGLDQKHKP
                     VTCQLWDREALDEPGAPKTPDGKRRMIAPSWSA"
     gene            complement(2680..3840)
                     /locus_tag="PK812_02330"
     CDS             complement(2680..3840)
                     /locus_tag="PK812_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRJ68417.1"
                     /translation="MSGTVLKTALLAALMSTTAIVGARADITVGFITSMSGPVSSIGI
                     PYSRGIKAGEAHIGEVGGQKFKVIALDDASDVSNASKLARKLIEEDKVDVLIGTAGAP
                     GSAAIMAVATEMKVPVVGITPVPGVPKNDGKPWALTVVQPASDMVGAVVQEMKAQNIK
                     NVGFIGFSDSWGDLVYNNGKAAADKLGINIVTNERYARADTSVSAQILKVVAARPDAV
                     LNGGSGTGGALPYTALAERGFKGPIFGTPALINPDFVRLAGAAGEGTVCSTGPVMVVN
                     QLPDAHPSKKIGLAFKEAHLKANGVASNDGFSAYAFDAWLVLADASKRALAKAKPGTP
                     EFRSALRDEMYQVKELAGSHGVFNFKEDSPYGTDMRALVLVKLQKGEWKFYK"
     gene            complement(3919..4704)
                     /locus_tag="PK812_02335"
     CDS             complement(3919..4704)
                     /locus_tag="PK812_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008597749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HRJ68418.1"
                     /translation="MSTPIRIWYQSFVDYENGKPYWDRLRQHISAVVDPGTTVEVHGI
                     TPFDNYAHAIVEYRCGREVICNAVRAEREGYDAFVIGHFQDAGLYEARACVDIPVVAL
                     GESSMLYACQLGQRIGIVTINTRFIPWFHHQIRKYGLEQRITGVHAMSFEPGQILEAY
                     ASAELAKEVERLFEKQAKPLIAQGVDVLLPGGGIPMLLFSDIKGHVIDGAPVVNGIPI
                     AVKMAEMAVKMRRLSGLGPSRVSDFFKPPVEIIDEFMAHPKGL"
     gene            4858..5907
                     /locus_tag="PK812_02340"
     CDS             4858..5907
                     /locus_tag="PK812_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRJ68419.1"
                     /translation="MDFQIAAILTVDGIANGAIYVLIALGTVLIFSVTRVMFVPFGDI
                     AAFAALTLAMLQMGRLPGTVLLVAVLAAIALAMELFHLWRHGKLAQTPRAVLNYAILP
                     CLPAAVAWLLEGVSLPLGGQMLLALALVIPIAPLLDRIAFQPLADASALVLLIVAVAV
                     HFAISGLALLFFGPEGFRTEPISRAVFDFGGVIVSAQTILMVLAAAAFSGLLYLYFEK
                     TIAGKALRATAVNRVGARLVGIRPQRTGTIAYLLASVLGGVSGLLIGPVATLYYDSGF
                     LIGLKAFVGAVVGGLMSYPLTAVGAIAVGILESFASFWSSTLKETIVFAALIPVLIWR
                     SLVSAHHEEEEEEIS"
     gene            5904..7673
                     /locus_tag="PK812_02345"
     CDS             5904..7673
                     /locus_tag="PK812_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HRJ68420.1"
                     /translation="MSLAAVSRWAPLLLAFVIAALPLVVSPFTVTLMNYIGIGAIVAL
                     GLVLMTGFGGLTSFGQAAFVGIGAYATAWATAVMGYPPLLGLALALVVSGAVALVLGA
                     ITLRLGGHFLPLSTIAWGIAIYFLFANIPGLGAHDGIRGIPPITLAGVSLAPNRAIYY
                     LIFALLAAAMWGVANLLDSREGRAIRSLRGGATMIASLGVDLFRMRLTIFLIAALLAG
                     LAGWLYAHMLRFISPSPFDIRPSIEYLLMAMAGGAGSVYGAIVGAAIVTFLKNWIQDI
                     LPYLSKNATQLEIVAFSVLLIILLQYARGGLLSLVQRFVPREARIPRAAQTGLARRAL
                     PQAGETLLKVEGATKRFGGLVAVNNVSFEMRAGEILGLIGPNGAGKSTLFNLVTGALR
                     MDGGSVAFMGRDVSNAPQASIARAGVARTFQHVKLRANMSLVDNVLLGAYPRTSAGFV
                     QCLLRLEREEEARATREALDQLDLVGLGAKAAELAGNLPLGNQRVLEIARALAADPIL
                     IVLDEPAAGLRKAEKDALAAQLRAIRARGVSILLVEHDMDFVMGLVDRIVVLDFGSKL
                     MEGTPADVRASAAVQEAYLGGVA"
     gene            7670..8389
                     /locus_tag="PK812_02350"
     CDS             7670..8389
                     /locus_tag="PK812_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ68421.1"
                     /translation="MSRLMEVTNLSVSYGKVEAVRAVSLTIDAGQIVTIIGPNGAGKT
                     TLLGAIMGLLPSLGSIVYEGRDLKGRDIEDRVFDGLCLVPERRELFGDLPVEDNLLLG
                     AYVHRRDSAHVKRSFEVVYDRFPRLKERRAQLAGTLSGGERQMLALGRALMSKPRLLM
                     LDEPSLGLAPLIVREIFKIIASLRDLGVSILLVEQNARAALETADFGYVLETGDVTLS
                     GKADILMHDPRVTATYLGGHG"
     gene            8386..9954
                     /locus_tag="PK812_02355"
     CDS             8386..9954
                     /locus_tag="PK812_02355"
                     /inference="COORDINATES: protein motif:HMM:NF004808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004467 - long-chain fatty acid-CoA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005324 - long-chain fatty acid
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent acyl-CoA ligase"
                     /protein_id="HRJ68422.1"
                     /translation="MMLPGFPWLPLPPEERTLARMLVGQAERYGSRRLFSCLGETWRF
                     DEAPGIAAHMAGALAEAGIQAGDRVAILSPNRPEVMRLLVGCGWLGAVAVPLNTAVKT
                     RQLRYYLENSGARLLVIDPQLLAALEPGAFDGLALDRIWVPGGDAPTPGAEPFRAEGP
                     AVPAHASSPGDPFAILYTSGTTGAPKGVVCPQAQFHWWGLLTARFLELREGDVLATPL
                     PLFHTNAINTFFQALLTGSTLEVLPKFSASGFWRAMSESGATVSYLLGAMVPMLLAQP
                     ESDLERAHRLRVALGPGVPAAMHERLFERTGVRLVDGFGSTETNFIIGGTAGERVPGR
                     MGRVAPGIAARVVDGLDEPVPDGTAGELVVRSDEPFSFASGYFGLPEKTVEAWRNLWF
                     HTGDRVIRHGDGTFAFIDRLKDVIRRRGENISSFEVEQVIAAFAEVETVAVFPVPSDL
                     AEDEVMAALVLKPGAALDAADFFARCARELPRHAVPRYVEVFAELPRTENGKVQKFKL
                     RERGLTPATLDRMA"
     gene            complement(9968..10699)
                     /locus_tag="PK812_02360"
     CDS             complement(9968..10699)
                     /locus_tag="PK812_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007009248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRJ68423.1"
                     /translation="MDGKRTALVTGGAAGIGAEIVRSLIADGFHVVSIDRHAPETGSP
                     DRTDILCDLIDPEATARAAAEVAARFSVTHFVHNAGTIRANLIESTSAADLAALSQLH
                     LGAAITLAQAILPAMKAAGSGRIVLMSSRAVLGAATRTVYSATKAGVIGLVRTWTLEL
                     ARHGITVNALAPGPIGGTDMFHDVMPKGDPRIAALAASIPMKRLGTPADVANAARFFL
                     SDASGFITGQTLYVCGGVSVGQAAF"
     gene            complement(10703..11479)
                     /locus_tag="PK812_02365"
     CDS             complement(10703..11479)
                     /locus_tag="PK812_02365"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007755719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HRJ68424.1"
                     /translation="MSGTLTAMLAAIAGGGVKVVDLTFTLSPEFPALVLPPELGQCAP
                     FRMEEVSRYDPRGPAWYWNNISMSEHCGTHYDAPIHWVTGKDLPNNSVDTIPVERFLA
                     PACVIDCSAQAAADPDFVLSTGDIAAFERDHGRIPAGWWVLMRTDWSKRDFTGYANLR
                     EDGAHTPGPSPEAMRFLVDERGILGFGTETIGTDAGQAHHFAPPYPAHHFLHGAGRYG
                     LQCLCNLDLLPPRGAVIVAAPLKIRQGSGSPLRVLALVEG"
     gene            complement(11476..12717)
                     /locus_tag="PK812_02370"
     CDS             complement(11476..12717)
                     /locus_tag="PK812_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRJ68425.1"
                     /translation="MTAPLPPPKRKNPLKRTRLPVSPPDLRSRQSHLLTRAAAEGRFG
                     LPTCLDCGALHYPPRDACPHCLSSRIGMRECSALGTLAATTTVQISNNVYFRERSPWR
                     VGLVTLDAGPGVVAHVHGDCADGGRVRLDWRLDRSGNATAFAVPDPPSPNMMDDPQMR
                     EMTLDPKHRRVLVTDGRTALGQAMAKALSDAGAALVFVGIAEAWKPFPGSERLSAMRG
                     VEIMPLDMTDTDSVNDLADAIGARVDIIVNTGAHIRPGGLLERKGVTVAREELEAGYL
                     ALLRLAQGFGPVMRFRGADGANSACAWVNVFSIYAQMAWPAYGAWSAAQAAMLNASLS
                     LRGELRPGGVRVVHVFTGPTEDEWFQPLPPPKVAAGQIASAVVDALRRGLEDVYVGDV
                     AQDFRARLDLNPKALERELGQ"
     gene            complement(12719..13900)
                     /locus_tag="PK812_02375"
     CDS             complement(12719..13900)
                     /locus_tag="PK812_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008597734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HRJ68426.1"
                     /translation="MARFAVPPRQGYGDVVVCAPVTVPYQRYSTESAHWWIGRALKAL
                     TETAGLRRQDLDGFLVSSFTLFPDTAIGLTQHYGLSPRWLDHIPMGGASGILALRKAA
                     RAVQAGDCAIAACVAGDTNHVDSFRKSLSTFSRFAQDAVYPYGSGGPNASFALICAHY
                     MRMYGATREDFGKLAVAQRANALRFPHALMRKPLTLDDYLSARMIADPIGLYDCVMPC
                     AGAEAFLVMREATARALGLPFARLLATIERHNAHAEDAIQHRGGWSVDIGELYTMAGI
                     APEALSFIQTYDDYPVISLMQLEDLGLCAKGEAKDFVRANSFTVDGTMPHNTSGGQLS
                     VGQAGCAGGFLGMVEAIRQLTGTALGGQVANARYGLVSGFGMITYDRGLASGAAILGG
                     A"
     gene            complement(13902..15536)
                     /locus_tag="PK812_02380"
     CDS             complement(13902..15536)
                     /locus_tag="PK812_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006938510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRJ68427.1"
                     /translation="MRETEPTTVHGALERAARLWPRNAFLNVLPETATIYAIEPGEIS
                     YADMAALVTEQIAAYRAAGVGAGHRLGLLVENRPVFFRHWFAMNALGASVVPINPDLR
                     AGELEYLIGHSEMSAAIVLPGRIADIAAAAKAAGRAIPVIAPDAALPRLDDARAPDPA
                     PGSETECALLYTSGTTGRPKGCVLTNTYFLNNGRWYATVGGLVRIRPGRERMLTPLPL
                     FHMNAMATSTMAMMMSGGCLTILDRFHPRTWWDSVRAARATIVHYLGVMPPMLMGAPP
                     SDRDSDNEVVFGFGAGVDKRLHGPFEARFGFPLVEAWAMTETGGGVIIAANREPRHLG
                     VSCIGRPDEVTELRIIREDGSDCGADEPGELLVRRKGPDPRHGFFREYLKDRQATEEA
                     WAGGYFHTGDVVRRDGDGYIYFVDRRKNVIRRSGENIAAVEVESVLQQHPAVAAVAVA
                     ATPDPVRGDEVFACIVAKEPPADAAGRRALAESLVQHALSRLAYYKAPGWVAFVPSLP
                     VTATQKIQRGAMKDLVAQLAGDPATVDTRAMKKRTA"
     gene            complement(15536..16018)
                     /locus_tag="PK812_02385"
     CDS             complement(15536..16018)
                     /locus_tag="PK812_02385"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008546673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HRJ68428.1"
                     /translation="MTQPTDKDLIDFVIREARLLDELKFEAWLTLFTEDGHYWMPAEW
                     GQTDARLVGSLMYEDKLLLTVRVERLKGNRTFSQKPKSRCHHVLQTPTVDARDDAKGE
                     YHLYTPFHYVETRLDEQTLYAGWARHHLVLQDGALKIRLKRVDLVNCDAAFGNIQLFM
                     "
     gene            complement(16027..17343)
                     /locus_tag="PK812_02390"
     CDS             complement(16027..17343)
                     /locus_tag="PK812_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HRJ68429.1"
                     /translation="MGKYRGNPDAVRALVRDAEVHRDVYIDQEIFELEMEHLFANTWV
                     HVGHESQVANPGDYFTTTIGSQPVIMVRHTDGTIKVLHNRCPHKGVKIAIDGCGNTGK
                     FFRCPYHAWTFKTDGSLLAIPLKKGYENTGFENSQAARGMACVKNVKNYRGFVFAKLN
                     DVGPDFDEFFGDSLSSFDNMIDRSPVGKLEVAGGVLRYMHKCNWKMLVDNQTDTCHPM
                     VAHESSAGTAAAVWKPFEGTGAKKPMAVEIFLPFISPYEFFEGMGIRIWDNGHGHTGV
                     GHSIHSNYTEAPGYFEAMKTAYGEERAKAILGENRHNTCYFPNVMIKGPIQLLRIFRP
                     LAADRTLVESYTYRLVGAPDMLLERTQMYNRLINAPTSVVGHDDLEMYERAQEGLRSN
                     GNEWTNVQRLYDPAEKEQTVAVTNGTNEWQMRNHFRAWAKFMTLSM"
     gene            complement(17367..18353)
                     /locus_tag="PK812_02395"
     CDS             complement(17367..18353)
                     /locus_tag="PK812_02395"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008597738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDR/VanB family oxidoreductase"
                     /protein_id="HRJ68430.1"
                     /translation="MPVLSLKVAGIRDETALIRRIVLTRSDGGALPGFEAGAHITLHA
                     PGIGRRKYSLVNADPTPGVTSAPSRYVLGIRRDDAGQGGSKWAHGLKVGDIIEAEPPQ
                     NDFELAAGEGPVLLIAGGIGVTPLIAKAAHMKALGRPFRFIYAAKSMAEMAFLPEIQA
                     LAGDALTLHADDMAGTFLDIRALIDTLTHDEPVYICGPKPMIKAAMQEARALGWPAGR
                     LRFELFFNAAAEAPPAPPAPDEGAFEVEIKSTGAVFKVPRDRTILAVLKEAGLDPLHD
                     CDKGECGVCQVGVLSGVPDHRDSLLSEAERAAGKTMQICVSRAKSSRLVLDL"
     gene            complement(18449..19243)
                     /locus_tag="PK812_02400"
     CDS             complement(18449..19243)
                     /locus_tag="PK812_02400"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HRJ68431.1"
                     /translation="MAKDLLTKLAKALAKRKIVVVDLTQRLHPSTPVIQLPPMFAPSA
                     PFSISEISRYDEKGPAWYWNNIACGEHTGTHFDAPVHWVSGKDLKDGATDTVDVQRMV
                     APACVIDVSRECRKDERFLLTPKHILAWEKKHGKIPAGSWVLMRSDWSKRTDPAKFLN
                     MKEDGPHTPGPNADAVRLLVEERDVNGWGVEAVGTDAGQAFSFEPAFPAHALMHGANK
                     FGLASLCNLDKLPPTGALLITPPLKITHGSGSPLRVLALVDSTDED"
     gene            complement(19263..20645)
                     /locus_tag="PK812_02405"
     CDS             complement(19263..20645)
                     /locus_tag="PK812_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013850798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRJ68432.1"
                     /translation="MNAAVEALRAALGDGIVRTGADIPARNLTDSSRYTPPTPPLALV
                     LPRSVEDVATALRLCHAHGQPVVTQGGMTGLADGATPARTEVAISLERMSGIEEIDVQ
                     AGTITALAGTPLEAIQKAADDAGFVCGIDLGARGSCTIGGNVATNAGGNQVLRYGMTR
                     RNVLGLEAVLADGTIVRSLNKMLKNNAGYDWTQLLFGSEGTLGIVTRVVVMLHPKPAS
                     IETALIALPSADAAIRLLRRAGAELPGGLLVFEAMWRDLIEVAIQHVKLPSPFAETPE
                     MTVLLEAPGDRSGQFRELLEGFLGSMLEEGIITDALVAKSGADRARFWAYRECMYEFH
                     RLMPPMVAFDVSLPIGSMREAVDVIRARLAARHGDAIVVNFGHIADSNLHLIVMKPGL
                     DKTAKLDIEAIVYAGVSEYGGSVSAEHGVGRAKRPYLTLSRTPEELALMRTIKQALDP
                     RGILNPERIL"
     gene            complement(20642..21460)
                     /locus_tag="PK812_02410"
     CDS             complement(20642..21460)
                     /locus_tag="PK812_02410"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HRJ68433.1"
                     /translation="MFDPRRASLRYRIASGRCIGVIWLAMGSAALVEVAARSKPDAIV
                     LDLQHGLFDRRELEAAIGIVPPEIPVIVRVAENSPFAIGMALDAGAEGVMVPLVESAK
                     QARNAVRYAKYPPDGVRSGGGVRPLGDFAEYVEGSDKIVVMVMIETDKGVKNARDIAA
                     VDGVDMVFIGTGDLSLSIGVFPNIHHDHTIACAAIHAACKKAWTPCGIFTPSLDMAKM
                     RRGQGYRMVVTANDMDILDRGFSKASTDFAATPPAPPAPVIEANAAPLRIGDDT"
     gene            complement(21453..21998)
                     /locus_tag="PK812_02415"
     CDS             complement(21453..21998)
                     /locus_tag="PK812_02415"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRJ68434.1"
                     /translation="MTSETAAVASSEAFRQGMRALAGSVCVVAANGADGAPVGLTATA
                     VTSLSADPPSLIVCVNRNASIAAALGQGAQFSVNLLALGQEEVAQAFGGQKAAKGVAR
                     FAFGDWIRSAQDIPLLMGANVSFECEVAQVTDWATHHIVIGLIRDARFAQAQTAALVY
                     HQGRYGSVGTTPRAPKEDGDV"
     gene            complement(22006..23100)
                     /locus_tag="PK812_02420"
     CDS             complement(22006..23100)
                     /locus_tag="PK812_02420"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRJ68435.1"
                     /translation="MLKPHPLKGPNRFKIGVFSANADGGLAITDVPERWRARWDDVVT
                     AAQIADRAGMEFFLPIARWRGFGGKNRVREHSFETFTFAAGLASATQQIGLFMTVHVP
                     IVHPVYAAKALATVDHISGGRAGLNIVCGWNPDEFAMFGIQLAGDAYVQAAEWVDVIA
                     QCYGTNQPFDFKGKFYDLKQVVSRPTSVQVPRPVTMNAAFGPPGRDFAARNCDYLFST
                     FSEIADGRQHVEDMRERAAAVGRNVGVYTVCHVVCRETQKEAEEYYTRYAVTEADHAA
                     VDYHMASKKEFANSHDGDAFNRYRKRFAGGAGSYPLVGTPEKIVDEMLKISREGYAGS
                     ALTFVNYSYELPFFCDRVLPLMKQAGLRVQ"
     gene            complement(23100..23843)
                     /locus_tag="PK812_02425"
     CDS             complement(23100..23843)
                     /locus_tag="PK812_02425"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRJ68436.1"
                     /translation="MTSAIAPRLAEHRRLVRRDNLSDRIYEDMRERLQRGEFDNRTRL
                     VDVDVAATYGTSRMPAREALLRLSNDGFLIGTTRGFVLPQLSLDDIREIFEVRRLLEP
                     RAAANAARDLTDEAQMRMAQALSMARIASQEDDVERLIESNMAFRMAWLDCVRNKRLA
                     NTIARFVDHVQTVRLGTLKDAVTRKVVADGLEGLYDAFVRRDPVAAADRMASFMAAAE
                     QAFFDIRKAEISGSSQDGEGRPQTWRSRA"
     gene            complement(23933..25507)
                     /locus_tag="PK812_02430"
     CDS             complement(23933..25507)
                     /locus_tag="PK812_02430"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRJ68437.1"
                     /translation="MFRTAFGSTSLALCMALAAPGLHAADLRLGVSSPISSIDPHFQN
                     LVPNLAVSAHIFDTLVAMTADAQIRPALAESLKPIDDTTWEVKLRKGVKFHDGSDFTA
                     EDVIFSLDRPPTVVKSPAPFTIYTRAITEKTVVDSHTLRLKTATPYPLLANDLAQVFI
                     VSKKASQNATTEDFNAGKAAIGTGPYRFTSYTAEDRVQMSANPSYWGGKPSWDNVTYR
                     FIASNSTRVAALLSGDVDAIDQVPTQDLERIKKDQNLTFADKPSLRIIYLYVDSGRDA
                     PPFVTAKNGQPLPKNPLKDLRVRQALSMSINRDAIRERIMDGLATPSSHFVPPPLRNN
                     EPGFDTVKFDPDAAKKLLAEAGYPDGFSITIHGPNNRFVNDDKIVQSVAQFFTRIGID
                     AKVDTMPMGMYATRGAKGEFALGLIGWGAQGGEVSSTLRAIIACANKDRGFGQFNWSN
                     YCNPKVDELLVKALATMDDAARLKTLNEASKMAANDGAIIPIHFQATTWAAKKGITIE
                     GRADERTYAMGFRPAR"
     gene            complement(25548..26933)
                     /locus_tag="PK812_02435"
     CDS             complement(25548..26933)
                     /locus_tag="PK812_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRJ68438.1"
                     /translation="MQAKTDRVLSHIDANLDQSLERLFALLRIPSISTDSAHAGDVAR
                     AAEHVARDARSIGFAAAPVPTPGHPAVLGHYRKNNRPHVLFYGHFDVQPVDPLALWDF
                     PPFEPKLVEVAPGRTVIRARGACDDKGQMMTFLEACRAWKAVTGDLPVDVSLVLEGEE
                     ENGSESLPEVLRAHRKALKADHCFICDTGQWDRDTPAISISLRGMFHEEIVVRTATTD
                     LHSGRFGGAANNAIRVLSRIVADLHAPDGSIAIPGFYDGIEDISPAMRTAWRRLGLTP
                     KKLLGPIGLSVPAGEKDRMVLEQISTRPTCDVNGIWGGYQGEGVKTVIPNEAHAKVSF
                     RLAAGQHPDRIRKLFRAFVRARLPKDCSVTFTNFKATTPANVRWDSDQVRRAGAALAA
                     EWGRKPVLMGQGGSIPIVNDFKKILGLEPLLIGFALKDDQVHAPNEKYDLQSFHKGIR
                     SWARILAAFAH"
     gene            27119..28285
                     /locus_tag="PK812_02440"
     CDS             27119..28285
                     /locus_tag="PK812_02440"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004770.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRJ68439.1"
                     /translation="MSLIRPAIEALKDSPIADVWRLGFTTDGVIGMWAGEPDVPTPDF
                     ICDAATAALRAGHTFYTHTRGIPELRQAIAGYLRRLWAADVADDRIAVTSAGMNAVML
                     VAQGLVQAGDNVVCVTPSWPNIMRAMQVAGADIREVAMRPGGNGWSLDIDAVINACDD
                     RTRLIYLATPGNPTGWMIERAEAERLLAFARRTGTALLSDEVYHRLVYDRPVAFSFLE
                     IARPEDPVFIVNSFSKSWAMTGWRMGWLIYPAGLEPQFEKLVQFNTSGGQAFLQHAGI
                     AAIRDGEPFVASFLERCRTGREIVNRRLARMPRVTNIPNTGAFYAMFEVEGVSDTLAF
                     CKRAVPEARIGLAPGVAFGKGAERLIRLCYAKSPANLEEAMDRLERYLATYKEA"
     gene            28287..29441
                     /gene="argE"
                     /locus_tag="PK812_02445"
     CDS             28287..29441
                     /gene="argE"
                     /locus_tag="PK812_02445"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF005710.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008777 - acetylornithine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HRJ68440.1"
                     /translation="MQPSARVLETLTSIIAFDSTTTKSNLDCIDWARGELERHGARTL
                     TDWNEDRSKANLFATFGEGAGGVVLSGHVDTVSVEGQDWSSDPFRLTIRDGRAYGRGT
                     CDMKGFDAVVIALAQRYGAARLKRPIHVALTYDEEQGCLGIPKLIAALDRAGIHPDGA
                     LIGEPTSMRLVSAHKGGEVYRVTVRGKEAHSSQTPKAVNAIEISAVLIAKIRQIARRH
                     QTDGPFAPEFVTPHTTLSTNLFSGGTGPNIVPNEARFLFDIRYIPGVDPAEIMAEIRA
                     ESAALAREMAALGVAEGIRFDRVGAIPALDARPSDAIYRTVHGLCADKGVDKVDYGTE
                     ASFFQAYGVPSVVCGPGSIAQAHKPDEFVPLADLAACERMIEGVVAACEN"
     gene            complement(29484..>30127)
                     /locus_tag="PK812_02450"
     CDS             complement(29484..>30127)
                     /locus_tag="PK812_02450"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRJ68441.1"
                     /translation="ALRALVIASSGNEAFCAGGDLQQMGDGAPDAFDAHGERGQFAAL
                     FRDLWDLGKPVLARVQGPAMAGGFGLAAACDLIIASEKASFGLPEVAVGIWPYMITVP
                     LLHAMAPRQALRLMMTGERIDAVEGQRLGFVTEVTPHAELDACIDRWIARLSAGSPQA
                     IALGRTAFYTALNHDMDARLRMLEALLTANLTMPDAAEGLAAFAEKRRPNWKT"
BASE COUNT         4729 a         9720 c        10412 g         5266 t
ORIGIN      
        1 ggcaacgaca ggcggtgcgc cgtccacggt cagatcccgc cagccatgag gtctgccatc
       61 tgcgcgcgat cgaggccatc gaggccgagt gtggcgaggg tgcggccgga cctgaggaaa
      121 tcaacgccgg ccgcgacgcc gccgatctgc aagaggcctt ccgccgtcgg cgcggggacg
      181 ccggcccatc gggcgatcga gacgagcagc gccaggccca gggcgacgtc ttccgccatg
      241 tagcgatggg tcgaaaggtt cagcgtctcg tgccagtctc ccgagccgac gagcttgtcg
      301 tgcgcgagat tgccatacat ccagttggcg gtggcatagt ggtcggccag cggaaaatgc
      361 ggcggacgat acccaagagc ctcgcgcagg gcgatcctct cgccgtcaag cgcgtcgtgc
      421 acgcggcgga ttgagggctg cgtgccttcc ttgtggatgt cccagcgttc gaaatgctcg
      481 atcggcccgg cgttcatcag gatcagcggc ggatggatga tcggcccggc attcatcaga
      541 gcggccgaga gcgcatcctc gaccggctcg atgcaggccg ggaaggcgcg gcggatcacg
      601 gcgagcgccg catcgctcag gcgtgctgga aagataccgg tcggcaggcg cgacgcccgt
      661 gttgtgaccg cgatctccgc cggtccgtgc ttgcgcgtca gccagggcag ggtgccggct
      721 tccccgatgg cgatgtcggc ggtcgaaccg gccgcgcgga tgacctgcag catcagcacc
      781 gaacccagcg tgccgggcgg aatgaagatc acctgcccat cggcaagatg cggggcgatt
      841 cgccgggcga tgtcggcctg ggcgaccgcc ggcagcggcg acaggataag gtcggcgccg
      901 tgcacggcct ggccaatgtc gagcgtcggc tgcgcgatgc ggacgtctcg gctgccgcgg
      961 aaatccttca ggcgcacggt tccggtcgcg agcaccgggg cgaaggcagc cgcatcctgc
     1021 cgccagaagc gcacctcgtg cccggcctcg ctcaggtcgg cggctgccgc gtagcagccg
     1081 tgtccaccgc cgatgaccgc gatcttcatg gcactggaca cggggacatc cttgctcacg
     1141 ccgaccagga gggcgcgatc atgcgccgtt tgccgtcggg cgtcttcggc gcgccgggct
     1201 cgtccagcgc ctcgcgatcc cagagctggc aggtcaccgg cttgtgcttc tggtcgaggc
     1261 cctcgcagta gttggtgtat tcgcacaggc gcacggcgcc gccgtggccg gagcgcacct
     1321 tgcggaacca gtccgggtcc gccagcgcct gtcgggcgag gccaacaatg tctgccttgc
     1381 cgtcggcgag gatcgcttcc gcctgcgcga agccgtgcac gccgccggcg gccaccaccg
     1441 gcgtttccaa ccctgtggcg cggatggcgg cgcgaacgcg tccaaccggc tcgacgttgc
     1501 ggccaaaggg accgaacgcg tcggaaatgt agcccggcat gcattcgtaa ccgctcgggc
     1561 cggtgtaggg ataggcggat tcgcccacct tcggctgctt ggcgtcatcg aacttgccgc
     1621 cgcgcgacag cgacaggaag tccatgccgg cgcgggcgaa ggcgatgccg atgccggtgg
     1681 cgtcctcgac cgtgttgccg ccctcaatgc actcgtcggc gagatagcgg cagccgagca
     1741 catagtcgtc gccgacggcc ttgcgggtgg cggcgtaaac ctccagcggc agccggagcc
     1801 ggttctccag ggcgccgcca tagccgtcgg atcggctgtt ggtccgcgag aggaacgagg
     1861 ccatggtgta ggcatgcgcg tagtgcagtt cgaccccgtc gaatccggcc tcgcgggcgc
     1921 gggtcgcggc gttggcgaag agaccgggca acacaacggg cagctcggcg atgtgcggca
     1981 ggtgcgtatc ggtcacgcgc tcgcgaaagc cgatggtcag atcctcgtat tcacgatcgg
     2041 tgaggatggc cttgagctcc gcgagcggca ttgaagccag cgccttgcgc acgtcagctt
     2101 ccggccagtc gcgcgcgccg agcgcgtcgc ggtggcgcgc gctgatcgtc aggaagcgtt
     2161 cgagaaactt ggccgggtcg ggccggcggc ggatgttcag gaagtcgatg atctggatga
     2221 acaggcgggt gtgcccgccg ctcgcctcgc gcacagcctc gaccagtcgc ttcagcccgg
     2281 ggatgaagcg ttcgtggccg atgcgcagca acggtcctga cggcacatcg cgaatgccgg
     2341 tcgcctcgac cacgatggcc ccggggcggc cctgcgcgaa gcgggcatac cagtcaaggt
     2401 tatcctgggt gacgaagccg tcctcggtcg cgcgccaggg caccatggcc ggcacccagg
     2461 tgcggctttc gagggcgagc gcccgatgcc ggacaggcga gaacaggcgt gaacccgccg
     2521 cttccgccgc cgtgggccag ggccccggtc tggtctcgtg cttgattttc tcaggcggtt
     2581 tccacatggc gctcaaaggg tcttttcgtc gtcaaagcca ttcggctccc ttctccgcca
     2641 acgggagaag ggagcggatt gttccaggca tgcggtggtc tacttgtaga acttccactc
     2701 gcccttttgg agcttcacca gcacaagggc gcgcatgtcg gtgccatacg ggctgtcttc
     2761 cttgaaattg aagacgccgt gtgagccggc cagttccttg acctggtaca tttcgtcgcg
     2821 cagcgcggag cggaactccg gcgtgccggg cttcgccttg gccagggcgc gtttcgacgc
     2881 gtccgccagc acaagccacg catcgaaggc ataggcggag aagccgtcgt tggaggcaac
     2941 gccgttggcc ttcagatgcg cttccttgaa ggcgaggccg atcttcttgg acgggtgcgc
     3001 gtccggaagc tggttgacca ccatgacggg accggtcgag cagaccgtgc cttcacccgc
     3061 agcgcccgcg agccgcacga agtcggggtt gatcagcgcc ggcgtgccga agattggtcc
     3121 cttgaagccg cgctcggcca gtgccgtata gggcagcgcg ccgccggttc ccgagccgcc
     3181 gttcaggaca gcatccgggc gggccgcgac caccttcagg atctgggcgc tgacggaggt
     3241 gtcggcgcgg gcgtagcgct cgttggtgac gatgttgatg ccgagcttgt cggccgctgc
     3301 cttgccgttg ttgtagacga ggtcgcccca ggaatcggag aagccgatga agccgacgtt
     3361 cttgatgttc tgggccttca tttcctgcac gacggcgccg accatgtcgc tggccggctg
     3421 caccacggtg agcgcccagg gcttgccgtc attcttcggc acgccgggaa cgggcgtgat
     3481 gccgacgacc gggaccttca tttcggtcgc gaccgccatg atcgcggcgg agccgggcgc
     3541 gccggccgtg ccgatcagga cgtcgacctt gtcctcctcg atcagcttgc gcgccagctt
     3601 cgaggcgttg gacacgtccg aggcgtcgtc gagcgcgatg accttgaatt tctggccgcc
     3661 gacctcgccg atatgcgcct cgcccgcctt gatgccgcgc gagtagggaa tgccgatcga
     3721 cgagaccggt cccgacatcg aagtgatgaa gccgaccgtg atgtcagcgc gcgcgccgac
     3781 aatggcggtc gttgacatca atgccgcgag cagcgccgtc ttgagtacag ttcctgacat
     3841 aggttcctcc accttcctgt tgtcacggac cgaccggacg gccggcccga atgccttgag
     3901 tgcggcggcg ggcgccgcct acagcccttt tggatgcgcc atgaactcgt cgatgatctc
     3961 gaccggcggc ttgaagaaat ccgaaacgcg actggggcca agtccactca agcggcgcat
     4021 cttcacggcc atttcggcca ttttgacggc gatcggaatg ccgttgacca cgggcgcgcc
     4081 gtcgatcaca tgccccttga tgtctgagaa gagcagcatc gggatgccgc cacccggcag
     4141 gagcacgtcg acgccctgcg cgatcagcgg cttcgcctgc ttctcgaaaa gcctctcgac
     4201 ctccttcgcc agctcggcgg aggcataggc ctcgaggatc tgtcccggct cgaacgacat
     4261 ggcatgaacg cccgtgatcc gctgctccag tccgtatttc cggatctggt gatgaaacca
     4321 cggaatgaag cgggtgttga tggtgacgat gccgatgcgc tgcccgagct ggcaggcata
     4381 gagcatggag gattccccga gcgcgaccac cgggatgtcc acgcaggccc gcgcctcgta
     4441 gagtccggcg tcctggaagt gcccgatcac gaaggcgtcg tagccctcgc gctctgctct
     4501 gactgcgttg cagatcacct cgcggccgca gcgatactcg acgatggcat gggcgtaatt
     4561 gtcgaaaggc gtaatgccgt ggacctcgac cgttgttcca gggtcgacaa ctgcggaaat
     4621 atgctgacgc aggcgatccc aatagggctt gccgttctcg tagtccacga agctctggta
     4681 ccagatacgg atcggcgtgc tcatggcggc cttcgggtta gggtgttccc ctcgaccgca
     4741 atcctagacc cgcgatattt gaaacgtaaa gtattttgtt gacgagtcgg cgcggccggc
     4801 caagattgta tccgatggtg cggctgggag gcatgcgccg gtgcaaggac gggcggaatg
     4861 gatttccaga ttgcggcgat cctgacggtg gatggcatcg ccaacggcgc gatctatgtg
     4921 ctgatcgcac tgggcacggt gctgatcttt tccgtcacgc gcgtcatgtt tgtcccgttt
     4981 ggcgacatcg ccgccttcgc cgctctgacg ctggcgatgc tgcagatggg ccggttgccg
     5041 ggaacggtgt tgctggtggc cgtgctggcg gccatcgctc tggctatgga gctgttccat
     5101 ctgtggcgac acggaaagct ggcgcagacc ccgcgcgcgg ttctcaacta cgccatcctg
     5161 ccctgcctgc cggctgcggt cgcctggctt ctggagggcg tgtcgctacc gctcggcgga
     5221 cagatgctgc tggcgctcgc cctggtgatc cccattgcgc cgctgctcga tcgcattgcc
     5281 ttccagccgc tggcggatgc ctctgcgctg gtcctgctga tcgtcgcggt tgccgtgcat
     5341 ttcgcgatct cgggactggc cctgctgttc ttcggcccgg aagggttccg aaccgagccg
     5401 atctcccgcg cggtgttcga tttcggcggg gtgatcgtca gcgcgcagac gatcttgatg
     5461 gtgcttgccg ccgcagcctt ctcgggcctg ctctacctgt atttcgagaa gacgatcgcg
     5521 ggaaaggcgc tgcgcgcgac ggccgtcaac cgggtcggcg cccggctggt gggcatccgg
     5581 ccgcagcgga ccggcaccat cgcctatctt ctggcctccg tgctgggcgg agtgtcggga
     5641 ctgctgatcg gtcccgtcgc gacgctctat tacgactccg gtttcctcat tggactgaag
     5701 gcgttcgtcg gcgcggtggt gggcgggctg atgagctatc cgctgacggc ggtcggggct
     5761 atcgcggtcg gcattctgga aagtttcgcc tcgttctggt cgagcacgct gaaggagaca
     5821 attgtgttcg cggcgctcat tccggttctc atctggcgct cgctggtcag cgcgcaccat
     5881 gaggaggaag aggaggaaat ctcgtgagcc ttgccgccgt gtcccgctgg gcgccgctgt
     5941 tgctggcttt tgtgatcgcg gcgctgccgc tggtcgtcag cccgttcacc gtgaccttga
     6001 tgaactacat cggcatcggc gcgatcgtcg ccctggggct ggtgctgatg accgggtttg
     6061 gcggcctgac ctcgttcgga caggcggcct ttgtcggcat cggggcctat gcgacggcgt
     6121 gggcgacggc ggtgatgggg tatccgccgc tgctgggcct cgcccttgcg cttgtcgtca
     6181 gcggcgcggt ggcgctggtg ctgggggcga tcacgcttcg gctcggcggc catttcctgc
     6241 cgctttcgac catcgcctgg ggcatcgcca tctacttcct gttcgccaac atcccgggcc
     6301 tgggggcgca tgacggcatt cgcggcattc cgccgatcac tcttgccggc gtgtcgctgg
     6361 cgccgaaccg ggccatctac tacctgatct tcgcgctgct ggccgcggcg atgtggggcg
     6421 tcgccaatct gctcgattcc cgcgagggcc gggcaatccg ctcgttgcgc ggcggcgcga
     6481 cgatgatcgc cagcctcggc gtcgacctgt tccgcatgcg actgacgatc ttcctgatcg
     6541 cggcgctgct tgccggcctc gccggctggc tctacgcgca catgctgcgc ttcataagcc
     6601 cctcgccctt cgacatccgg ccgagtatcg aatacctgct gatggcgatg gctggcggcg
     6661 ccggctcggt ctatggcgcc atcgtcggcg cggcgatcgt gacgttcctg aagaactgga
     6721 ttcaggacat tctgccctat ctgtcgaaga acgcgacgca gcttgagatc gtcgcctttt
     6781 cggtgctgct gatcatcctt ttgcaatatg cgcgcggtgg actgctgtcg ctggtgcagc
     6841 gcttcgtgcc gcgcgaggcc cgaattccga gggcggcgca gaccggactt gcccgccgcg
     6901 cgctgccgca ggccggcgag acgctgctca aggtcgaagg cgcgacgaaa cggttcggcg
     6961 gcctggtcgc ggtcaacaac gtgtcgttcg agatgcgggc cggagaaatt ctgggcctga
     7021 tcggacccaa cggcgcgggc aagagcacgt tgttcaacct cgtcaccggc gcgctgcgca
     7081 tggatggcgg ctcggtcgcg ttcatggggc gcgacgtcag caacgcgccg caggcgagca
     7141 tcgcgcgggc cggagtggcg cgcaccttcc agcacgtgaa actgcgggcc aacatgagcc
     7201 tggtcgacaa cgttctgctg ggcgcctatc cgcgaacctc cgccggcttc gtgcagtgcc
     7261 tgctgcggct cgaacgggag gaggaggcgc gcgccacgcg cgaggcgctg gaccagctcg
     7321 acctggttgg ccttggagcg aaggccgccg aactcgccgg caacctgccg ctcggcaacc
     7381 agcgcgtgct ggagatcgcc cgggcgctcg ccgccgatcc gatcctgatc gtgctggatg
     7441 agcccgcggc gggactgcgc aaggcggaaa aggacgccct ggcggcgcag cttcgcgcca
     7501 tccgcgcgcg cggcgtgtcg atcctgctcg tcgagcacga tatggacttc gtcatgggcc
     7561 ttgtcgaccg catcgtcgtg ctcgatttcg gctccaagct gatggagggc acgcccgccg
     7621 acgtgcgcgc cagcgcggcg gtgcaggaag cctatctcgg aggcgtcgca tgagccggct
     7681 catggaggta acgaacctgt cggtctccta cggcaaggtc gaggcagtgc gcgccgtgtc
     7741 gctgacgatc gatgccggcc agatcgtcac catcatcgga ccgaacgggg cgggcaagac
     7801 gacgttgctc ggcgcgatca tggggctgct gccttcgctg gggtccatcg tctacgaggg
     7861 ccgcgacctg aaagggcgcg acatcgagga tcgcgtgttc gacggactgt gccttgtgcc
     7921 ggagcggcgc gagttgttcg gtgacctgcc ggtggaggac aacctgctgc ttggcgccta
     7981 tgtgcatcgc cgcgacagcg cgcatgtgaa gcgcagcttc gaggtggtgt acgaccgctt
     8041 cccgcgcctc aaggagcgcc gcgcgcagct cgccggcact ctctcgggcg gcgaacgcca
     8101 gatgctggcg ctgggccgcg cgctgatgtc gaagccgcgc ctgttgatgc tggacgaacc
     8161 ctcgctcggc ctggcgccgt tgatcgtgcg cgagatcttc aagatcatcg cgtctctgcg
     8221 cgatctcggc gtgtcgatcc tgctggtcga gcagaatgcg cgcgccgcgc tggagacggc
     8281 ggatttcggc tatgttctgg agaccggcga cgtcacgctg tcgggcaagg ccgacatctt
     8341 gatgcatgat ccccgggtca ccgcgactta ccttggcggg catggatgat gttgccggga
     8401 tttccctggc tgccgctgcc gcccgaggag cgaacgctgg cgcgcatgct tgtcggccag
     8461 gcggagcgtt acgggtcgcg ccggctgttt tcctgccttg gcgaaacctg gcggtttgac
     8521 gaggctcccg gcattgccgc ccacatggca ggtgcgctgg cggaagccgg catccaagcc
     8581 ggcgaccgcg tcgccatcct gtcgcccaac cggcccgagg tgatgcgcct gcttgtcggc
     8641 tgcggctggc tgggcgccgt cgccgttccg ctcaacaccg cggtgaagac gcgccagttg
     8701 cgctattatc tcgaaaactc cggggcgcgg ctgctggtca tcgatccgca gctgctggcg
     8761 gctcttgagc ccggggcctt cgacggtctg gcgctggacc gcatctgggt tccgggcggc
     8821 gatgccccaa cgcccggggc cgaacccttc cgtgccgagg ggcctgcggt tccggcgcat
     8881 gcgtcaagcc cgggtgatcc gttcgcgatc ctctatacct ccggcacgac cggcgcgccc
     8941 aagggcgtgg tctgcccgca ggcgcaattc cactggtggg gattgttgac ggcgaggttc
     9001 ctggaactgc gtgaaggcga tgtgctggcg acgccgctgc cactgttcca caccaacgcc
     9061 atcaacacat tctttcaggc gcttctgacc ggatcgacgc tggaggtgct gccgaaattc
     9121 tccgcctccg gcttctggcg cgccatgagc gagagcggcg cgaccgtcag ctacctgctc
     9181 ggcgcgatgg tgccgatgct gctggcgcaa ccggaatcgg accttgagcg ggcgcaccgg
     9241 ttgcgcgtgg cgctcggtcc cggcgttccg gccgccatgc acgagaggct gttcgagcgc
     9301 accggcgtcc ggctggtcga tggatttggt tcgaccgaga cgaactttat catcggcggc
     9361 acggcgggcg agcgtgtgcc cggccggatg ggccgcgtcg caccgggcat cgcggcacgt
     9421 gtcgtggatg ggctcgatga accggttccg gatggcacgg ccggcgaact ggtggtgcgg
     9481 tcagacgagc cgttcagttt cgcctccggc tacttcggcc tgccggagaa gaccgtggag
     9541 gcctggcgca acctgtggtt ccacaccggc gaccgggtga tccggcatgg cgatggcacg
     9601 ttcgccttca tcgaccgtct gaaggacgtc atccgccggc gcggcgagaa catctcttcc
     9661 ttcgaggtcg agcaggtcat cgcggccttc gcggaagtgg aaaccgtcgc ggtcttcccc
     9721 gtgccgtccg atctggcgga agacgaggtc atggcggccc tggtgctgaa gcccggcgcg
     9781 gcccttgacg cggcggattt cttcgcccgc tgcgcgcgcg agctgccgcg ccatgcggtg
     9841 ccgcgttatg tcgaggtctt cgccgaactg ccccgcaccg aaaacggcaa ggttcagaag
     9901 ttcaagctgc gcgaacgtgg cctgacgccc gcaacgctcg atcgcatggc gtgagcgaga
     9961 gccggcttca gaacgctgcc tgtccgacac tgacgccgcc gcagacgtag agtgtctggc
    10021 cggtgatgaa ccccgaggcg tcggacagga agaagcgcgc ggcattggcc acgtccgccg
    10081 gcgtgcccag ccgcttcatc ggaatggatg cggcgagcgc cgcgatgcgc gggtcgccct
    10141 tcggcatgac atcatggaac atgtcggtgc cgccgatcgg ccctggcgcg agcgcattga
    10201 cggtgatgcc gtgccgggcg agttccagcg tccaggtccg cacaagcccg atgacgccgg
    10261 ccttggttgc ggaatagacc gtgcgtgtcg cggcacccag cacggcgcgc gaggacatca
    10321 gcacgatccg gccgctgccg gccgccttca tcgccggcag gatcgcctgc gcgagtgtta
    10381 tggccgcgcc gagatgcagc tggctcaacg ctgcaaggtc ggcagccgag gtgctctcga
    10441 tcaggttggc gcggatcgtg cccgcgttgt gcacgaaatg cgtcacggag aaccgcgccg
    10501 cgacctcggc tgcggcgcgc gccgtcgcct ccgggtcgat gaggtcgcag aggatgtccg
    10561 tgcgatccgg actgccggtc tccggcgcat ggcgatcgat gctgaccaca tggaatccgt
    10621 cggcgatcag actacggacg atctccgcgc cgatgccggc tgcgccgccg gtgacgagcg
    10681 ccgtgcgctt gccgtccatg gttcagccct ccaccagcgc gagcacgcgc agcggcgaac
    10741 cggaaccctg ccggatcttg agcggcgccg cgacgatcac cgctcctcgc ggcggcagca
    10801 gatcgaggtt gcagaggcat tgcaggccat agcgaccggc cccgtgcagg aagtggtgcg
    10861 ccgggtaggg cggggcaaag tggtgcgcct gccccgcgtc cgtcccgatg gtttccgtgc
    10921 cgaagccgag gatgccgcgc tcgtcgacaa gaaatcgcat cgcttccggc gacggtcccg
    10981 gtgtatgggc gccgtcctcg cgaagattgg catagccggt gaaatcacgc ttcgaccagt
    11041 ccgtccgcat cagcacccac cagccggccg gaatgcggcc gtggtcgcgc tcaaaggccg
    11101 cgatgtcgcc ggtgctcagc acgaagtcgg ggtcggccgc cgcctgggcc gaacaatcga
    11161 tgacgcaggc cggcgcgagg aaccgctcga cgggaatcgt atcgaccgag ttgttcggca
    11221 ggtccttgcc cgtcacccag tggatcggcg cgtcataatg cgtgccgcaa tgttcggaca
    11281 tggagatgtt gttccagtac caggccgggc cgcgcggatc gtagcgcgac acttcctcca
    11341 tgcggaacgg cgcgcactgg ccgagttccg gcggcaggac cagcgccgga aactccggcg
    11401 agagtgtgaa ggtgaggtcc accaccttca cgccgccgcc ggcgatcgcg gcgagcatcg
    11461 cggtcagggt tccgctcatt ggccaagctc ccgttccagc gccttggggt tgaggtcgag
    11521 gcgggcccgg aagtcctgcg ccacatcccc gacatagacg tcctcaaggc cccggcggag
    11581 cgcatcgacg acggcggagg cgatctgtcc ggccgcgacc ttgggtggcg gcaacggctg
    11641 gaaccattcg tcttcggtgg gcccggtgaa gacatgcacc acccgcacgc cgccgggccg
    11701 aagttccccc cgcagcgaca gcgaagcgtt gagcatcgcc gcctgcgcgg cggaccaggc
    11761 gccataggcg ggccaggcca tctgcgcata gatggaaaag acattcaccc aggcgcaggc
    11821 gctgttggcc ccgtccgcgc cacggaaacg catcaccgga ccgaagccct gagccagcct
    11881 gagcagcgcg agatagccgg cttccagctc ttcgcgcgcg acggtgacgc ccttgcgttc
    11941 gagcaggccg cccggacgga tgtgcgcgcc ggtattgacg atgatgtcga cccgcgcgcc
    12001 gatggcgtca gcgaggtcgt tgaccgagtc ggtatcggtc atgtcgagcg gcatgatctc
    12061 gacgccccgc atggcgctca gccgctccga gccgggaaac ggcttccagg cctcggcgat
    12121 gccgacgaag acgagcgccg ctccggcatc ggaaagggcc ttcgccatgg cctgaccgag
    12181 cgcggtgcgc ccatcggtga ccagcacgcg ccggtgcttg ggatcgagcg tcatctcgcg
    12241 catctgcggg tcgtccatca tgttcgggct cggcggatca ggcacggcga aggccgtcgc
    12301 gttgccgctg cggtcaagcc gccagtcgag gcgcacgcgc ccgccgtccg cgcagtcgcc
    12361 gtggacatgc gcgacgacgc ccgggccggc gtccagcgtc accagcccga cgcgccaggg
    12421 gctgcgttca cggaagtaga cattgttgga aatctgcacc gtggtggtcg ccgcgagcgt
    12481 gccgagcgcg gaacactcgc gcatgccgat gcgggacgac aggcagtgcg gacaggcatc
    12541 gcgcggggga tagtgcaatg cgccgcagtc gaggcaggtc ggcaggccga agcggccctc
    12601 cgccgcggcg cgggtaagca ggtgggattg ccggctgcgc aggtcgggcg gggagaccgg
    12661 caggcgggtg cgcttcagcg ggttcttgcg cttgggcggg gggagcgggg cggtcatgtc
    12721 aggcgcctcc cagaattgcc gcacccgacg cgaggccgcg atcgtaggtg atcataccga
    12781 agccggaaac gaggccgtag cgcgcattcg ccacctgccc gcccagggcg gttccggtga
    12841 gttgccggat cgcctcgacc attccgagga agccgccggc gcagcccgcc tgcccgacgg
    12901 acaattgccc gcccgaggtg ttgtgcggca tcgtgccatc cacggtgaag ctgttcgccc
    12961 gcacgaagtc cttcgcctcg cccttggcgc agaggccgag gtcctccagt tgcatcagcg
    13021 agatgacggg gtagtcgtcg taggtctgga tgaaggacag cgcctccgga gcgatgccgg
    13081 ccatcgtgta gagttcgccg atgtccacgc tccagccgcc ccgatgctgg atcgcgtcct
    13141 ctgcatgggc gttgtggcgt tcgatggtcg cgagaaggcg cgcgaagggc agtccaagcg
    13201 ccctggccgt ggcttcgcgc atcaccagaa aggcctcggc accggcgcag ggcatgacgc
    13261 aatcatagag cccgatgggg tcggcgatca tgcgcgcgga aagatagtcg tcgagcgtca
    13321 gcggctttct catcagcgcg tggggaaatc gcagcgcatt ggcacgctgg gccacggcaa
    13381 gcttgccgaa atcctcgcgc gttgccccgt acatgcgcat gtaatgggcg cagatcaggg
    13441 cgaacgaggc gttcggcccg ccggatccgt agggatagac cgcgtcctgc gcgaagcggg
    13501 agaaggtcga gagcgacttg cggaaactgt cgacgtggtt ggtgtcgccc gcgacgcagg
    13561 cagcgatggc gcagtctccg gcctgcacgg cccgggcggc cttgcgcagg gcgagaatgc
    13621 cgctcgcccc gcccatggga atatggtcga gccagcgtgg cgacaggcca taatgctgcg
    13681 tcaggccgat ggcggtgtcc gggaacagcg tgaatgagga aaccaggaag ccgtcgaggt
    13741 cctgccggcg cagcccggcg gtctcggtca gcgctttcag cgcccgtccg atccaccaat
    13801 gggcgctctc ggtggaatag cgctggtagg gcacggtgac cggcgcgcac accacgacat
    13861 cgccgtagcc ttgccggggc ggaacggcga agcgcgccat gtcaggccgt ccgcttcttc
    13921 atggcgcggg tgtcgaccgt cgcgggatcg ccggcaagct gcgccaccag atccttcatc
    13981 gcgccgcgct ggatcttctg cgtcgcggtg accggcagtg aggggacgaa ggcgacccag
    14041 cccggcgcct tgtagtaggc cagccgcgag agcgcgtgct ggaccaggct ttcggccagg
    14101 gcgcggcggc cggcggcgtc cgctggcggt tcctttgcca cgatgcaggc gaacacctcg
    14161 tcgccgcgca ccggatcggg ggtcgccgcc accgccaccg ccgccaccgc aggatgctgc
    14221 tgcagcacgc tttcgacctc caccgcggcg atgttctcgc ccgagcggcg gatgacgttc
    14281 ttgcggcggt cgacgaagta gatgtagccg tcgccgtcgc ggcgcaccac gtcgccggta
    14341 tgaaagtagc cgccggccca ggcctcttcg gtcgcctggc gatccttcag gtattcccgg
    14401 aagaagccgt ggcgcgggtc cggtcccttg cggcgcacca gcaattcgcc cggctcgtcg
    14461 gcgccgcagt cgctgccgtc ctcccggatg atgcgcagct ccgtgacctc gtctggccgg
    14521 ccgatgcagg agacgccgag atggcgcggt tcccggttgg cggcgatgat gacgccgccg
    14581 ccagtctccg tcatcgccca ggcctcgacc agcgggaagc cgaagcgtgc ctcgaacggg
    14641 ccatgcaggc gcttgtcgac gcctgcgccg aagccgaaca ccacctcgtt gtcgctgtcc
    14701 ctgtcggagg gcggtgcgcc catcagcatc ggcggcatga cgccgaggta atgcacgatg
    14761 gtggcgcgcg cggcgcgcac gctgtcccac caggtccggg ggtggaagcg gtcgagaatg
    14821 gtcaggcagc cgccggacat catcatcgcc atggtcgagg tcgccatggc gttcatgtgg
    14881 aacagcggca gcggggtcag catgcgttcg cggccggggc ggatccgcac caaaccgccg
    14941 accgtggcat accagcgccc gttgttgaga aagtaggtgt tggtcaggac gcagcccttg
    15001 ggccgcccgg tcgtgccgga ggtgtagagc agcgcgcatt ctgtctcgga gcctggcgcc
    15061 ggatcgggtg cgcgggcgtc atcgaggcgc ggcagcgcgg catccggcgc gatgacggga
    15121 atcgcgcggc ctgcggcttt cgcggcggcg gcgatgtcgg cgatccggcc cggcaggacg
    15181 atcgccgcgc tcatctcgga atggccgatg agatattcga gttcgccggc cctcagatcc
    15241 gggttgatcg gcacgacgga ggcaccgagc gcgttcatcg caaaccagtg ccggaaaaac
    15301 accggccggt tctccaccag caggcccagg cggtggcccg cgcccacgcc ggcggcccgg
    15361 taggcggcga tctgttccgt gaccagtgca gccatgtcgg catagctgat ctcgccgggc
    15421 tcgatggcgt agatggtggc cgtctcgggc agcacattga ggaaggcgtt gcgcggccac
    15481 aggcgggccg cgcgctcaag ggcgccatga acggttgtcg gttccgtctc gcgcatcaca
    15541 tgaacagctg gatgttgccg aaggcggcgt cgcagttcac caggtcgacg cgtttcagcc
    15601 tgatcttcag cgcgccgtcc tgaagcacca ggtgatgcct ggcccagccg gcgtaaagcg
    15661 tctgctcgtc gagccgcgtc tccacgtagt ggaacggggt gtagaggtga tactcgccct
    15721 tcgcgtcgtc acgggcgtcg acggtcgggg tctgcagcac gtgatggcag cggcttttcg
    15781 gcttctgcga gaaggtgcgg ttgcccttga ggcgctcgac gcgcacggtg agcagcagct
    15841 tgtcctcgta catcaacgag ccgacgagcc ttgcgtcggt ctggccccac tcggccggca
    15901 tccagtaatg gccgtcttcg gtgaagagcg tcagccacgc ctcgaacttc agttcgtcca
    15961 gcaggcgggc ttcgcggatg acgaagtcga tgaggtcctt gtcggtcggt tgtgtcatgg
    16021 cgcgcctcac atcgacagcg tcatgaactt ggcccaggcg cggaagtggt tgcgcatctg
    16081 ccattcattg gtgccgttgg tgaccgcgac cgtctgctcc ttctcggcgg gatcgtagag
    16141 tcgctgcacg ttggtccatt cgttgccgtt ggacctgagg ccctcctgcg cccgctcgta
    16201 catctcgagg tcgtcgtggc cgaccaccga ggtcggcgcg ttgatcagcc ggttgtacat
    16261 ctgcgtgcgc tccagcagca tgtccggcgc gccgaccagg cgataggtgt agctttccac
    16321 cagcgtgcgg tccgccgcga gcgggcggaa gatgcgcagg agctggatcg gccccttgat
    16381 catgacgttg gggaaatagc aggtgttgtg gcggttctcg ccgaggatcg ccttggcgcg
    16441 ctcctcgcca taagcggtct tcatggcctc gaaatagcca ggcgcttcgg tatagttcga
    16501 gtggatggag tggcccacgc cggtgtggcc gtgtccgttg tcccagatgc ggatgcccat
    16561 gccctcgaag aactcgtaag gtgagatgaa gggcaggaag atttccaccg ccatcggctt
    16621 cttggcgccg gtgccctcga agggcttcca taccgccgcc gccgtgccgg ccgagctctc
    16681 atgcgcgacc atcgggtggc acgtgtcggt ctggttgtcg accagcatct tccagttgca
    16741 cttgtgcatg tagcgcagca cgccgccggc aacttccagt ttcccgaccg gcgagcggtc
    16801 gatcatgttg tcgaaggagg agaggctgtc gccgaagaac tcgtcgaaat ccggcccgac
    16861 gtcattcagc ttggcgaaga cgaagccgcg atagttcttc acgttcttca cgcaggccat
    16921 gccccgcgcc gcctggctgt tctcgaagcc ggtgttctcg tagcccttct tcagtgggat
    16981 cgcgagcagg ctgccgtcgg tcttgaaagt ccaggcgtgg taggggcagc ggaagaactt
    17041 tcccgtgttg ccacagccat cgatggcgat cttgacgccc ttgtgcgggc agcggttgtg
    17101 caggaccttg atggtcccgt cggtgtggcg caccatgatc accggctgcg agccgatggt
    17161 ggtggtgaag tagtcgccgg gattggcgac ctggctttca tgaccgacat ggacccaggt
    17221 gttggcgaag agatgctcca tctccagttc gaagatttcc tggtcgatgt agacgtcccg
    17281 gtgcacctcg gcgtcgcgca cgagtgcccg caccgcatcg gggttgccac gatatttgcc
    17341 catgtcaggc ctccggttcg acccctctag agatcaagca ccagtctgga agatttcgcg
    17401 cgggagacgc agatttgcat tgtctttccc gccgcgcgct cggcctcgct caggagggaa
    17461 tcccggtgat ccggcacgcc ggaaagcacc cccacctgac agacgccgca ctctcccttg
    17521 tcacaatcat gcagcgggtc gagacccgcc tctttgagca ctgccaggat ggtcctgtcg
    17581 cgcggcacct tgaacaccgc acctgtgctc ttgatttcca cctcgaaagc gccctcgtcc
    17641 ggagcaggcg gggcgggcgg cgcttccgcc gcagcattga aaaacagttc gaatcgcagc
    17701 cgcccagccg gccagccgag ggcgcgcgct tcctgcatcg ccgccttgat catcggcttc
    17761 gggccgcaga tgtaaaccgg ctcgtcgtgg gtgagcgtgt cgatcagggc gcggatgtcg
    17821 agaaacgtcc ccgccatgtc gtcggcatga agcgtcagcg catcgccggc gagcgcttgg
    17881 atctccggca ggaaggccat ctccgccatg ctctttgcgg cataaatgaa gcggaacggc
    17941 cggcccagcg ccttcatgtg cgccgccttc gcaatcagcg gggtcacgcc gatgccgccg
    18001 gcgatcagca gcaccggccc ttcgccggcc gccagttcga aatcgttctg cggcggctcg
    18061 gcctcgatga tgtcgccgac cttgagccca tgcgcccatt tcgagccacc ctggccggca
    18121 tcgtcgcgcc ggatgcccag gacatagcgg gagggggccg acgtgacgcc tggcgtcggg
    18181 tccgcgttga cgagcgaata cttgcgccgt ccgatgccgg gcgcgtgcag ggtgatgtgc
    18241 gcgccggcct cgaatccggg cagcgcgccg ccgtcgctcc tggtgagcac gatgcgccgg
    18301 atcagggccg tttcgtccct gatcccggca accttgagcg agagcactgg catgcaccat
    18361 ccggaatgcg gtccgccggg aaggcggaca ggagcagata ttcgaagcct aaagcatttc
    18421 ggccgtgcag gacaaggggc gtctcgtctc agtcttcgtc cgtgctgtcg accagcgcca
    18481 gcacgcgcag cgggctgccc gatccatgcg tgatcttgag aggcggcgtg atcagcagcg
    18541 ctcccgtggg cggcagcttg tcgaggttgc acaggctggc cagcccgaac ttgttggcgc
    18601 catgcatgag ggcgtgggcc gggaaggccg gctcgaagga gaaggcctgg cccgcgtccg
    18661 tgccgaccgc ctcgacgccc cagccgttga cgtcgcgctc ctcgaccagc aaacgcaccg
    18721 catcggcgtt cggacccggc gtatggggtc cgtcttcctt catattgagg aacttggccg
    18781 gatcggtgcg cttcgaccag tccgaccgca tcagcaccca ggagccggcg ggaattttcc
    18841 cgtgtttctt ttcccaggcc aggatgtgct tgggcgtcag caggaagcgt tcatccttgc
    18901 ggcattccct tgagacgtcg atcacgcagg ccggcgcgac catgcgctgt acgtcgacgg
    18961 tgtcggtcgc cccgtccttc aggtccttgc cggaaaccca gtgcaccggt gcgtcgaaat
    19021 gcgtgccggt atgctcgccg caggcgatgt tgttccagta ccaggccggg cccttctcgt
    19081 cataacgcga gatctcggag atcgagaacg gcgccgaggg ggcgaacatc ggcggcagct
    19141 ggatgaccgg cgtcgagggg tgcaggcgtt gcgtcaggtc gacaacgacg atcttgcgtt
    19201 tggccagcgc cttcgccagc ttcgtcaaca ggtccttggc cattttgtcc tcccggaagt
    19261 gttcaaagga tacgctcagg attgaggatg cccctggggt cgagcgcctg cttgatggtg
    19321 cgcatcagcg ccagttcctc aggggtgcgc gacagagtca gatagggccg cttcgcccgg
    19381 ccgacaccgt gctccgcgga gacggagccg ccgtattccg acacgccggc ataaacgatg
    19441 gcctcgatgt cgagcttcgc ggtcttgtcg aggcccggct tcatgacaat gaggtgcagg
    19501 ttggaatcgg cgatatggcc gaaattgacg acgatcgcat cgccatgacg ggccgccagc
    19561 cgcgcgcgga tgacgtccac cgcctcccgc atgctgccga tcggcaagga tacgtcgaag
    19621 gcgaccatcg gcggcatcag ccggtggaac tcgtacatgc attcgcgata ggcccagaac
    19681 cgcgcgcgat ccgcgcccga tttcgccacc agcgcgtcag tgatgatgcc ctcctccagc
    19741 atggatccga ggaagccttc cagcagttcg cggaactggc cggagcggtc gcccggcgct
    19801 tccaggagca cggtcatttc cggcgtctcg gcgaagggcg acggcagttt cacatgctgg
    19861 atcgcgacct cgatcaggtc gcgccacatc gcttcgaaca cgagcagccc gcccggcagt
    19921 tcggctccgg cgcggcgcag caggcggatc gccgcgtcgg ccgagggcag ggcgatcagc
    19981 gccgtctcga tcgaggccgg cttggggtgc agcatcacca cgacgcgcgt gacgatgcca
    20041 agcgtgccct cggagccgaa cagcaactgc gtccaatcgt agcccgcgtt gttcttcagc
    20101 atcttgttga gagaccgcac gatcgtcccg tccgcgagca ccgcctccag gccgagaacg
    20161 ttgcgccgcg tcataccgta gcgcagcacc tggttgccgc cggcattggt ggcgacattg
    20221 ccgccgatgg tgcaggagcc gcgagcgccc agatcgatgc cgcagacgaa tccggcgtcg
    20281 tccgcagcct tctggatcgc ctccagcggc gttccggcca gcgccgtgat ggtgccggcc
    20341 tggacgtcga tttcctcgat gccgctcatg cgctccagcg agatcgcgac ctcggtgcgg
    20401 gcgggcgtcg cgccgtcggc gaggccggtc atgccgccct gggtcaccac gggctggccg
    20461 tgggcatggc agaggcgcag cgccgtcgcc acatcctcga cgctgcgcgg cagcacaagc
    20521 gccagcggcg gcgtcggtgg cgtgtagcga ctggaatcgg tgaggtttcg tgccgggatg
    20581 tcggcgccgg tgcgcacgat cccatcgccg agagcggcgc gcagggcttc tacggcggcg
    20641 ttcatgtgtc gtctccgatc cgcagcggtg cggcgttggc ctcgatgacc ggtgccggcg
    20701 gtgcgggcgg ggtcgcggcg aagtctgtgg aggccttgct gaacccccgg tccaggatgt
    20761 ccatgtcgtt ggccgtcacc accatgcggt aaccctggcc gcggcgcatc ttcgccatgt
    20821 cgaggctcgg cgtgaagatg ccgcagggcg tccaggcctt cttgcaggcc gcgtggatcg
    20881 ccgcgcaggc gatggtgtgg tcatggtgga tgttgggaaa gacgccaatc gacagggaca
    20941 ggtcgccggt gccgatgaag accatgtcca cgccgtcgac ggcggcgatg tcccgggcgt
    21001 tcttcacgcc cttgtcggtc tcgatcatca ccatgacgac gatcttgtcc gatccttcca
    21061 catactcggc gaaatcgccc agcgggcgca cgccgccgcc ggagcgcacc ccgtccgggg
    21121 gatacttggc gtagcgcacg gcattgcgcg cctgtttggc cgattccacc agcgggacca
    21181 tcacgccttc cgcgccggcg tccagcgcca tgccgatcgc gaagggcgag ttttccgcca
    21241 cgcgcacgat caccggaatt tccggcggca cgatgccgat ggcggcctcc agctcccggc
    21301 ggtcgaacag cccgtgctgg agatcgagca cgatggcatc gggcttggac cgggcggcaa
    21361 cctcgacgag tgccgccgat cccatggcca gccagatcac gccgatgcag cgtccggacg
    21421 cgatgcgata gcgcagcgag gcgcgtcggg gatcaaacat cgccatcctc ctttggtgcg
    21481 cggggggtgg tcccgacact gccgtagcga ccctgatggt agacgagcgc ggcggtctgg
    21541 gcctgggcga aacgggcatc gcggatcagg ccaatgacga tgtggtgcgt cgcccagtcg
    21601 gtgacctgcg ccacctcgca ctcgaacgag acgttcgcac ccatcagcag gggaatgtcc
    21661 tgcgccgagc ggatccagtc gccaaaggcg aagcgcgcca cgcccttggc cgccttctgg
    21721 ccgccgaagg cctgcgcaac ctcctcctgg ccgagcgcca gcaggttcac cgagaactgc
    21781 gcgccctggc cgagcgcggc ggcgatgctg gcgttgcggt tgacacagac gatcagggac
    21841 ggcgggtcgg ccgacagcga tgtcacggcg gttgcggtca ggccgaccgg cgccccgtcg
    21901 gcgccgttcg cggcgaccac gcagaccgaa ccggcaaggg cgcgcatgcc ctgccggaag
    21961 gcctcgctgc tggcaaccgc ggccgtctcg ctcgtcacgg tgtcctcatt gcacgcgcag
    22021 gccagcctgc ttcatcagcg gcaagacccg atcgcagaag aacggcaact cgtagctgta
    22081 gttgacgaag gtcagcgcgc tgccagcgta gccctcgcgc gagattttca gcatctcgtc
    22141 gacgattttt tctggtgttc cgaccagagg ataggagccg gcaccgccgg cgaaacgctt
    22201 gcgatagcgg ttgaaggcgt cgccatcgtg ggaattggcg aattccttct tgctcgccat
    22261 gtggtagtcc accgccgcgt ggtccgcctc ggtcacggca tagcgggtgt aatactcctc
    22321 ggcttccttc tgtgtctcgc ggcagacgac atggcagacc gtgtagacgc cgacgttgcg
    22381 tccgacagcg gccgcgcgct cgcgcatgtc ctcgacatgc tgccggccgt cggcgatctc
    22441 ggaaaaggtc gagaacaggt agtcgcagtt gcgcgcggcg aagtcgcgtc ccggcggacc
    22501 gaaggcggcg ttcatggtga cggggcgcgg cacctgcacg ctggtgggcc ggctgacgac
    22561 ctgcttcaga tcgtagaact tgcccttgaa gtcgaaaggc tggttcgtgc catagcactg
    22621 cgcgatgacg tcgacccatt cggcggcctg gacataggcg tcaccggcca actggatgcc
    22681 gaacatcgcg aattcatcgg gattccagcc acagacgatg ttgagcccgg cgcgtccacc
    22741 gctgatgtgg tcgacggtcg cgagcgcctt tgcggcatag accgggtgca cgatcggtac
    22801 gtgcacggtc atgaacaggc cgatctgctg ggtggcggac gccaggccgg cggcgaaagt
    22861 gaaggtctcg aaggaatgct cgcgcacccg gttcttgccg ccgaagccgc gccagcgggc
    22921 gatcggcagg aagaattcca tgccggcccg gtccgcgatc tgcgcggccg tgacaacgtc
    22981 gtcccagcgc gcgcgccagc gttccggcac gtcggtgatg gcaaggccgc cgtcggcgtt
    23041 ggcggagaac acgccgatct tgaaccggtt cgggcctttc agcggatggg gtttgagcat
    23101 catgcgcgcg atctccaggt ttgcggacgc ccctcgccgt cctggctgct gccgcttatt
    23161 tcggcttttc tgatgtcaaa gaaggcctgc tccgcggcgg ccatgaagga ggccatgcgg
    23221 tcggctgcgg ccacggggtc gcggcgcacg aaggcatcgt agagaccttc cagcccgtcg
    23281 gcgacaacct tgcgcgtcac cgcatccttg agcgtgccga gccgcacggt ctggacgtga
    23341 tcgacgaacc gggcgatggt gttggccagc cgcttgttgc gcacgcagtc gagccaggcc
    23401 atgcggaagg ccatgttgga ttcgatcagc cgctcgacat cgtcctcctg tgaggcgatc
    23461 cgcgccatcg acagggcttg cgccatgcgc atctgcgcct cgtcggtcag gtcgcgcgcg
    23521 gcatttgcgg cggccctcgg ctccagcagg cgccggactt cgaagatctc gcggatgtca
    23581 tcgagcgaga gctgcggaag tacgaagccg cgtgtcgtcc cgatcaggaa tccgtcgttc
    23641 gagagccgca gcagcgcctc ccgggcgggc atgcgcgagg tgccataggt ggccgccacg
    23701 tcgacgtcca ccaggcgcgt gcggttgtcg aattcgccgc gctgaaggcg ctcgcgcatg
    23761 tcctcgtaga tccgatccga caggttatcc ctgcgcacca ggcggcggtg ttcggcgagg
    23821 cggggagcaa tggcggaggt catgacggat tttcttgtat aatgaataca gtatatggaa
    23881 agtccggccg gtcaagggaa gccacgcctg accggccgga atggttactc cgtcagcggg
    23941 cgggccggaa gcccatggcg taggtccgct catccgcccg gccttcgatg gtgatgccct
    24001 tcttggccgc ccaggtggtg gcctggaagt ggatcgggat gatggcgccg tcattggccg
    24061 ccatcttcga ggcttcgttg agtgtcttca ggcgtgccgc gtcatccatg gtcgcgagtg
    24121 ccttgaccag cagttcgtcg accttcgggt tgcagtagtt ggaccagttg aactggccaa
    24181 aacccctgtc cttgttggcg caggcgatga tcgcgcgcag cgtcgaggaa acttcgccgc
    24241 cctgcgcgcc ccagccgatg aggccgagcg cgaattctcc cttggcgccg cgggttgcgt
    24301 acatgcccat gggcatggtg tcgaccttgg cgtcgatgcc gatgcgggtg aagaactgcg
    24361 ccacggactg cacgatcttg tcatcgttga cgaaacggtt gttcggcccg tggatggtga
    24421 tcgagaatcc gtcggggtag ccggcttccg ccagcagctt cttcgctgcg tccggatcga
    24481 acttgacggt gtcgaagccg ggctcgttgt tgcgcagcgg cggcggaacg aaatggctgg
    24541 agggcgtcgc aagcccgtcc atgatgcgtt cgcggatggc gtcgcggttg atggacatcg
    24601 acagggcctg acgcacgcgc aggtccttca gcgggttctt cggcagcggc tggccgttct
    24661 tggcggtgac gaatggcggt gcgtcgcgac cggagtcgac atagaggtag atgatgcgca
    24721 gcgagggttt gtccgcgaag gtgaggttct gatccttctt gatgcgttcg aggtcctgcg
    24781 tggggacctg gtcgatggca tcgacgtcac cggacagcag cgccgcgacg cgggtgctgt
    24841 tcgaggcgat gaagcgatag gtcacgttgt cccaggatgg cttgccgccc cagtaggacg
    24901 ggttggcgct catctggacg cggtcctcgg cggtgtagga ggtgaatcgg taaggcccgg
    24961 ttccgatggc ggccttgccc gcgttgaaat cctcggtggt ggcgttctgc gatgccttct
    25021 tcgagacgat gaacacctgc gccagatcgt tcgcgagcag cggatagggt gtggcggttt
    25081 tcaggcgcag agtatgggaa tcgacgaccg tcttctcggt gatcgcgcgg gtatagatgg
    25141 tgaagggggc cgggctcttg accaccgtcg gcggccggtc gagcgagaag atcacgtcct
    25201 ccgccgtgaa gtcggagccg tcatggaact tgacgccctt gcgcagcttc acttcccagg
    25261 tcgtgtcgtc gatgggcttc agggattcgg ccagcgccgg cctgatctgc gcgtcggccg
    25321 tcatcgccac cagcgtgtcg aagatatgcg cgctgaccgc gagattgggc acgaggttct
    25381 ggaagtgcgg atcgattgac gagatcggcg aggatacgcc gaggcgcaag tcggcggcgt
    25441 gaagccccgg cgcagcaagt gccatgcaaa gtgccagcga tgtgctgcca aacgcggtgc
    25501 ggaacattat tccctcctgt gttctggtgg cgtgcgatca ccaccggtta gtgcgcgaag
    25561 gccgcaagaa tccgggccca gctgcgaatg cctttgtgaa acgactgcag atcgtacttt
    25621 tcgttcggcg cgtgcacctg gtcatccttc agcgcgaagc cgatgagcag aggctcaagt
    25681 ccgaggattt tcttgaagtc gttgacgatg ggaatggagc cgccctgacc catcagcacc
    25741 ggcttcctgc cccattccgc cgcgagggcg gcgccggcgc gtctgacctg gtcgctgtcc
    25801 cagcggacat tggccggtgt cgtcgccttg aagttggtga acgtcaccga acagtccttc
    25861 ggcaggcgcg cccgcacgaa ggcgcggaac agcttgcgga tgcgatccgg gtgctggccc
    25921 gccgcgaggc ggaacgacac cttggcgtgg gcctcgttcg gaatgacggt cttcacgccc
    25981 tcgccctgat agccgcccca gatgccgttg acgtcgcagg tcgggcgcgt ggaaatctgt
    26041 tcgaggacca tgcggtcctt ctcgccggcc ggaaccgaca atccgatggg gccgagcagt
    26101 ttcttcgggg tcaggccgag ccgtctccat gccgtgcgca tggctggaga gatgtcctcg
    26161 atgccgtcat aaaagccggg gatggcgatg gatccgtccg gcgcatgcaa atctgcgacg
    26221 atgcgcgaaa ggacccggat ggcgttgttc gcggcgccgc cgaaccggcc ggaatgcagg
    26281 tcggtcgtgg ccgtccgcac cacgatctcc tcgtggaaca tgccccgcag cgagatcgag
    26341 atcgccgggg tgtcgcgatc ccattgcccg gtgtcgcaga tgaagcagtg gtctgcctta
    26401 agcgccttgc gatgggcacg caggacctcg ggcaggctct cagagccgtt ttcctcctcg
    26461 ccttccagca caagcgagac atcgacggga agatcgcccg tcacggcctt ccaggcgcgg
    26521 caggcctcga ggaaggtcat catctggccc ttgtcgtcgc aggcgccgcg cgcgcggatc
    26581 accgtccggc ccggagcgac ttcgacgagt ttcggctcaa agggcggaaa gtcccagagc
    26641 gccagcgggt cgacgggctg gacgtcgaaa tgcccgtaga acagcacatg cggacgattg
    26701 ttcttccgat agtgcccaag cacggcggga tggccggggg tgggcacggg agctgccgca
    26761 aacccgatgc tgcgggcatc gcgcgccacg tgctcggccg cccgggcgac gtcccccgca
    26821 tgggcgctgt cggtcgagat cgagggaatg cgcaacagcg cgaacagccg ttcgagcgac
    26881 tggtcaagat tggcatcgat gtgcgacaga acgcggtcag tcttggcctg catatgggct
    26941 ccggcgccgg gtgatcaggg gcctcggaaa actgaattca atattccgta gtggggcatg
    27001 tcaagagatg gttggcggcc aaggcctgcc attttccgac tgcgtgacga tcttgaccgc
    27061 cggccatggc ctgtagtgta ttcggtttga gtgtgtcccg gccgtttcac ggagcgtcat
    27121 gagcctgatc cgccctgcca tcgaagccct taaggattcg cccatcgccg atgtctggcg
    27181 actggggttc accaccgacg gtgtcatcgg catgtgggcc ggcgaacccg atgtgccgac
    27241 gcccgacttc atttgcgacg cggcgaccgc cgcgctcagg gctggccaca cgttctacac
    27301 ccatacgcgc ggcattcccg aactgcggca ggccatcgcc ggctacctca ggcgcctgtg
    27361 ggctgccgat gtcgccgatg accgcatcgc ggtcacctcc gccggtatga acgcggtgat
    27421 gctggtggcg cagggcctcg tgcaggccgg cgacaacgtg gtctgcgtga cgccgtcctg
    27481 gcccaacatc atgcgggcca tgcaggtcgc cggggcggac atccgggagg ttgcgatgcg
    27541 tccgggcggc aatggctggt cgctcgacat cgacgccgtg atcaacgcct gcgatgaccg
    27601 cacgcgtctg atctatctgg cgacgccggg caatcccacg gggtggatga tcgagcgcgc
    27661 cgaggcagaa aggctgctgg ccttcgcgcg gcgcaccggc acggcgctcc tgtcggacga
    27721 ggtctatcac cggcttgtct atgaccgccc ggtggccttc tcattcctgg agatcgcgcg
    27781 gccggaagac ccggttttca tcgtcaacag cttctccaag tcctgggcga tgaccggctg
    27841 gcgcatgggc tggctgatct atccggcagg gctcgaaccg cagttcgaaa aactcgtgca
    27901 gttcaacacc tcgggcggac aggccttcct gcaacatgcc ggaatcgcgg cgatccgcga
    27961 cggcgagccg ttcgtcgcct cgttcctgga gcgctgccgc acgggccgcg agatcgtcaa
    28021 ccgccggctc gcgaggatgc cgcgcgtcac caacattccc aataccggcg ccttctatgc
    28081 gatgttcgag gtggagggcg tcagcgacac gctcgccttc tgcaaacgtg ccgttccgga
    28141 ggcgcgcatc gggctggcgc cgggcgtcgc cttcggcaag ggtgcggaac gtctgatccg
    28201 gctgtgctac gccaagtcgc cggccaatct ggaagaggcg atggaccgtc tcgaacggta
    28261 cctcgcgacc tacaaggaag cctgacatgc agccgagcgc gcgcgtcctg gagacgctga
    28321 cgtcgatcat cgccttcgat tcgaccacga cgaagtccaa cctcgactgt atcgactggg
    28381 cgcgcggcga gctggagcgc catggcgcgc gcacgctgac cgactggaac gaggaccgca
    28441 gcaaggccaa tcttttcgcc accttcggcg agggcgcggg cggcgtcgtg ctgtccggcc
    28501 atgtcgacac ggtatcggtc gaggggcagg actggtcgag tgatccgttc cggctgacga
    28561 tccgcgacgg cagggcctat ggccggggga cctgcgacat gaagggcttc gacgcggtcg
    28621 tcatcgccct ggcccagcgc tatggcgcgg cgcggctgaa gcggccgatt catgtggcgc
    28681 tgacctatga cgaggaacag gggtgcctcg gcattcctaa actcatcgcc gcgctggaca
    28741 gggcaggcat ccaccccgac ggcgcgctga tcggcgagcc gacctcgatg cggcttgtca
    28801 gcgcccacaa gggcggcgaa gtctatcgtg tcacggtgcg cggcaaggag gcgcactcct
    28861 cgcagacgcc gaaggccgtc aacgccatcg agatctcggc cgtgctgatc gcgaagatca
    28921 ggcagatcgc ccgccggcat cagaccgacg ggccgttcgc gcccgagttc gtcacaccgc
    28981 acacgacgtt gtcgacaaac ctgttttccg gcggaaccgg accgaacatc gtgccgaacg
    29041 aggcgaggtt cctgttcgac atccgttaca ttcccggagt cgatccggcc gagatcatgg
    29101 cggagattcg cgccgaaagc gccgcgctgg ctcgggagat ggcggcgctc ggcgtggcgg
    29161 agggcatccg ctttgatcgt gtcggtgcca ttcccgcgct cgacgcgcgc ccctccgacg
    29221 ccatctaccg cacggtgcac gggctgtgtg ccgacaaggg cgtggacaag gtcgattacg
    29281 gcaccgaagc ctcgttcttc caggcctatg gcgtgccgtc cgtggtctgc ggtcccggct
    29341 ccatcgcgca ggcgcacaag ccggacgagt tcgtgccgct cgccgatctc gcggcctgcg
    29401 agcggatgat cgagggggtt gttgcggcct gcgaaaactg acggctgccg cggcggcgca
    29461 aggtgcccgg gccctccgcc gggtcaggtc ttccagttcg ggcggcgctt ttcggcgaag
    29521 gctgcgagcc cctcggctgc gtccggcatg gtgaggttcg cggtcaacag cgcttccagc
    29581 atgcgcaggc gcgcgtccat gtcgtggttg agcgcggtgt agaaggccgt gcgacccagg
    29641 gcgatggcct gcggtgagcc ggccgagagc cgggcgatcc agcggtcgat gcaggcgtcg
    29701 agctcggcat ggggtgtcac ctcggtgacg aagccgaggc gctggccttc cacggcatcg
    29761 atgcgctcgc ccgtcatcat caggcgcagc gcctgccggg gcgccatggc atgcagcagg
    29821 ggcacggtga tcatgtaggg ccagatgccg accgccacct ccggcaggcc gaagctggcc
    29881 ttctcgctgg cgatgatgag atcgcaggcg gcggcgaggc cgaagccgcc ggccattgcg
    29941 ggtccctgca cgcgggccag caccggcttg ccgaggtccc acaggtcgcg gaacagggcg
    30001 gcgaactggc cgcgctcgcc atgggcgtcg aaggcgtcgg gcgcgccgtc gcccatctgc
    30061 tgcaggtcgc cgccggcgca gaaagcctca ttcccgctgc tggcgatcac cagcgcgcgc
    30121 agcgccc
//
LOCUS       DAOMXN010000012        29349 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_1042_length_29349_cov_16.435140, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000012 DAOMXN010000000
VERSION     DAOMXN010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 29349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29349
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1042_length_29349_cov_16.435140"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1366
                     /locus_tag="PK812_02455"
     CDS             <1..1366
                     /locus_tag="PK812_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012253904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HRJ68442.1"
                     /translation="AAQAQPVRYEVQNPAPVQPAGKSEEYFATKSMVFGAMIEAIDLS
                     QLSKLDAEAAREELRDIVTEIINLKNVVLSIAEQEELLEDICNDVLGYGPLEPLLARD
                     DIADIMVNGANRTFIETGGKIQLTNVRFRDNAQLMNICQRIVSQVGRRVDESSPICDA
                     RLLDGSRVNVIAPPLAIDGPALTIRKFKKDKLTLDNLVKFGSISAEGADILKIIGRVR
                     CNVVISGGTGSGKTTLLNCLTRYIDHDERVITCEDAAELQLQQPHVVRLETRPPNLEG
                     NGQVTMRDLVKNCLRMRPERIIVGEVRGPEAFDLLQAMNTGHDGSMGTLHANNPRECL
                     SRLESMITMGGFTLPIKTIREMICASVDVIVQATRLRDGSRRITHITEVMGMEGDVII
                     TQDIILYNIEGEDANGKIIGRHASTGIGRPRFWERARYYGETERLAAALDALSSKPGE
                     SQAA"
     gene            1378..2352
                     /locus_tag="PK812_02460"
     CDS             1378..2352
                     /locus_tag="PK812_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRJ68443.1"
                     /translation="METRQLLIMAFAAVAAGGFAYAFIEPRMTGEVTAEARQKKLAGA
                     RVRGGDNLGKTAGRIKQVSETLKEVERKKAANDKVTLHDKIVQAGLDWERQRFMLVSM
                     ACAVVMGFVSLIISGNLFVGIGAFIVGGLGLPQWLLIFLRNKRVSAFTTELPNALDVI
                     VRGLRSGLPLGDCIRIIANEAAEPVKTEFRQIVEAQSIGLTIQEAVQGISKRVPTPEA
                     NFFSIVIEIQSKAGGNLSEVLSNLSKVLRERKKMKGKVAAMSMEAKASAAIIAALPFI
                     VAILVYISSPDYMSRLWTTEAGKITMVVSGFWMLIGIMSMKKMINFDL"
     gene            2365..3351
                     /locus_tag="PK812_02465"
     CDS             2365..3351
                     /locus_tag="PK812_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRJ68444.1"
                     /translation="MEGNIVSLILSPRFMGGVFTAIFVGAAILTVALPLLDRSDFKNR
                     MNAVALEREAIRQRERERMLQVRGTGRGNLKHEHKAFVKGFVEKFDLQRYIGADEMKA
                     KLAMAGYRGAPAEYGFMFFRFIMPIVLLLITALYVFVILDLSNYTVPMKIGMCLAGTY
                     LGFKAPEIFLSNQISKRQQNMQRAFPDSLDLLLICVEAGMSIEHAFRRVSVEIVGQSV
                     ALAEEYALVTAELSYLPDRRTAYENLAIRTGMESIKSVSTALVQAEKYGTPVGSALRV
                     LAQEGRDMRMNEAEKKAAALPPKLTVPMILFFLPVLFAVILTPALTQVFGWQ"
     gene            3452..4930
                     /locus_tag="PK812_02470"
     CDS             3452..4930
                     /locus_tag="PK812_02470"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRJ68445.1"
                     /translation="MNVRFVGYFLAGALPKLAMFLALFLLARHLDPAETGLFVLVITL
                     GEVIEMTAGTWLRIYVLKAEAGRKTIGPYRVGRFLVLALAMLAMALLVSLPIAAIIAP
                     GRFWTFAGATCAYIAAFAALRHAHTVLQILESHVVFILAEIARSLLIVACVLGSLATW
                     PTSFVAPSLLVSAATAATAVISLLLVASRLHGPRFTLAGIQPAVIFGAPIIADVVMSL
                     VIINFDRFVLNQMIGPAAVGIYALAYALGRQPIEFIAGPLNTYALPVLFAGYEHGGEE
                     RARQILTGLCITLFILCAAVLTGITLLEQPIANVALRMEYRDAAGLLMPVITFAACLL
                     VFKAFIFDNVFHMLQKNDEKLASVALATGVGVAATILLVYVAGINGAAAALVLSSGVG
                     LAASVYVSRRFFVFAIPLRHFAMIALAASGSGVALSLASWAVSAYGDGARIGAGFLAF
                     CLCYALMLKLQGISLKALASTPWEPFDARTGSERRPSDSNLR"
     gene            complement(4831..6021)
                     /locus_tag="PK812_02475"
     CDS             complement(4831..6021)
                     /locus_tag="PK812_02475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRJ68446.1"
                     /translation="MARGTQARTPGVIHLISSLRIGGAERLLVSTMTAAADGAAIRPV
                     VIVMNEAIDPGLMAELRGSGVPVYHLARREGHLHPRYLAAILRVIRTHDIEAIHAHND
                     GSLFWGMLAKLARPRLKLVYTQHEQGGALRIRGLSRLAYRAMVDMTVAISPFIVEETR
                     AITGDRVTLIENGIDLRKFIRSPAYQTPGRPVHLVQVGRFAPVKGQDVLLRAVRLCLD
                     RGLNIDCTLAGSVTDPAYFEMVTSLIKELDLENHVRIQLDRTDVENLLSDMDIFVLSS
                     RQEGFGIAIIEAMAANLPVVVPSVGGARDIVRHGDNGLLFEVGNPRDLADKLQHLIAT
                     PDLGAKFAAAGARTAARYDIRKTLEAKVTLYRKLLSDGRRSLPVRASNGSQGVDAKAL
                     REMP"
     gene            complement(6024..6782)
                     /locus_tag="PK812_02480"
     CDS             complement(6024..6782)
                     /locus_tag="PK812_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007606847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HRJ68447.1"
                     /translation="MRVFLFDTPVDILSRQETIDRAVAAMRTKVPCQHVALNVAKLVN
                     ARTDAELRQDVATADVVGIDGMGIVYALRLLGHRVDERVAGVDLFEDLMDLCAREGFR
                     PFLLGATQEVLTATQAELRRRHPDLRFAGCHHGYFKTEDDAAIADAIRESGADCVFVA
                     MPTPRKERFMKAWRARMGAPFVMGVGGTFDVVSGHVRRAPVIWQRLGLEWAYRMMQEP
                     RRMFWRYARTNAIFAVMLTRSMMLRLAGRIEKAA"
     gene            complement(6852..8639)
                     /gene="asnB"
                     /locus_tag="PK812_02485"
     CDS             complement(6852..8639)
                     /gene="asnB"
                     /locus_tag="PK812_02485"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HRJ68448.1"
                     /translation="MCGFAGLVQRTQDETAAARFRALALKSLARRGPDAAESAVIGPV
                     EMIHTRLSIIDVEGGRQPMEDEAGAIAFNGEIYNHADLRQPNTLYRTRSDTEVLLKGL
                     NTQGMDFLNRADGMFAFAYYNKAQRKVYLARDAFGIKPAYFVAKRDVFAFSSTIPALM
                     AFSEKRINAEGYLQFYMARAARGANTLFDDVHEVPPGHVAVYDIDANTLELTRWLKRE
                     PVAVSRKSEAELLDELDQLLHFSVRRHLVSDVPVASLLSGGIDSGLLTAIAARHVPDL
                     ACFSIGFKDQRFDESPYSQAIARKYGLRHFVKFVDGDDLVGMMEDWPSIADDASAEPA
                     SVMRYLVAQFARESGFKVVLSGEGADEFFGGYHQYFRFKLARQLNAFGRFAPFVGTLA
                     KDLLWPKSRLVHHLLQVTGTPEYHGTSMIFEPHMLGEVFAAKKVGYPVAHGLKDALRL
                     DQQFRLADDLLTVTDRSTMHASIEARVPFITRYVADFAASLPEHMIIKGRHKKYLLRQ
                     LALKYLPAECVTRRKVGFDMPLGRWLRNELRPLVEETLATSWQRDFLRKGAMEKIVAA
                     HMSGKADFADKIWAFITLERNVRALRAMA"
     gene            complement(8779..10104)
                     /gene="wecC"
                     /locus_tag="PK812_02490"
     CDS             complement(8779..10104)
                     /gene="wecC"
                     /locus_tag="PK812_02490"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008126140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HRJ68449.1"
                     /translation="MFMSAPQLNRQESVQAQRPAASSRIATVAVVGLGYVGLPTAALL
                     ASRGLNVVGVDVKRAAVERINEGRAHISEPDLDMVLNAAVKSGMLKAYTEAQPADVFL
                     IAVPTPITDEKTADMSMVESALASIAPVLKKGDLVVIESTSPVGTTERAAEQLRRLRP
                     DLTYPDTMPEVSDIMMAYCPERILPGQTLRELVDNARIVGGLDHRSAVRARDVYRHFA
                     MGKIKLTTSRVAELSKLTENAFRDVNIAFANELSLVCDHLGVDVWSVIKMANLHPRVN
                     ILSPGPGVGGHCIPVDPWFIHEALPEATPLIRNAREVNDAKPKRVADQISRLANRYRE
                     PVVALLGLSYKPNVDDLRESPALEIAEILSQRGIGRLLIVEPNIEKLPKSLASRPGVE
                     FMDHHAAIAAADVVAILVAHSKFRNADRAALASKVVIDTVGLLQQQDDE"
     gene            10348..12285
                     /locus_tag="PK812_02495"
     CDS             10348..12285
                     /locus_tag="PK812_02495"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HRJ68450.1"
                     /translation="MNVAKLRWYAARLAAMNPAEISHRFGELAKKRAWRRDDRGWNTY
                     SNVGDGRVLDIPELRNRIASGWPAARAGLSVFREGRLCGLGREVPATLGAAVWFTDPV
                     RGVRWPGAEKYCFDIDVRSTGETIGDVKFVWEINRLQFLHPLACAIATGTDEAAIAQT
                     LDMLADWRAANPPYRGVNWISGIELAMRLVSLTLVTAAIPQDRMSAGSRKLIRQLTAA
                     HGFWLQRYPSRHSSANNHLVAEGLGLFLAAHLAPDLPQARHWESEGRSILEAEAALQI
                     LPDGVGAEQSPTYQAFTMEMLAFAALIGELSHMPLASAVYERLRAGASFLRTLMDESG
                     GVPQIGDDDEGRVIAAPPDREPRYVASVTAAVAGLLRDDGLLPPSRQVHWRDALFNVP
                     ARTARPASGLSQFMEGGYSVGREEVNGRRCVLTFDHGPLGYLSLAAHGHADALAVWLS
                     IDGQPVIVDAGTFLYHSGRSMRAQLRASNAHNTLTVQGVSQSLPSAAFSWSTKAATRL
                     DETRPGRWWSLTGSHSGYERRFGLRHVRSVTRTPTGFAISDQLEGARKQFDVEIGFLV
                     HPAIAVVRENDRFVLLSNDTAIAEVVTPVSLFARIVRDDRETGMGLHSPRFGELVVAP
                     CIVASGTMSADPVVTRLNILA"
     gene            complement(12295..14412)
                     /locus_tag="PK812_02500"
     CDS             complement(12295..14412)
                     /locus_tag="PK812_02500"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide transport family protein"
                     /protein_id="HRJ68451.1"
                     /translation="MTFLVKNDIRGSQLMAMQAALPGLAGYAPLPPADTLTIDFRHLF
                     RGLARRKFWIVVPFLLFSAIGLLAALQVQPRYTAMVQVLVDPREIQVVRPDANLRSQV
                     PEAVNSVAENAIVMLRSSSTIASVVDRERLFDDEEFTGKQPRFGKPFDQSTEGRRLRA
                     IASLEKRVGARRADRSSVVEATVWTQDPVKSARIANAVAAVFLESQTSAENDTAKKAS
                     HAVTSRLEELAVRVQKAEREVEEYKARNNLQTANGRLVGEQQLQELNSQLVLARARTS
                     EARSKFDGVRKLSVQAIERGELPDTAQAGVLSQLRLKYAEAQRLEADARTKLGAKHPD
                     MIALAAQTRDMRSLVLDELNRISKSAQAEFERARASEESLSKSLDQLKMQSSDMGEAS
                     VRLRELERQAEATRTIYSSFLKRARELSEQEDLTTLNARIISSATPPHQSAGVSRSLI
                     LAGSMVAGLMIGLMLALLLEQFDSTLRDRKQFQLASNLPVLGEMPARRRKDGGVVAHV
                     IESPAAPFSLGACRVADTFAAHALPDRARSVLFLTVGRKTSATEIVLNVAIAAAQATW
                     RVLMIDGDASGNGLSAQLDHQPHAGLAEVVDRRVSLAGAVLTDERSGLRILPVCGPQG
                     GARRPSPQQLQANVLVPANSFELVFIDGGSLEQGNSALAFAGAVDDIVLVAGSGRTST
                     RELQEAVEALRSVASKLRGIVTA"
     gene            complement(14432..15892)
                     /locus_tag="PK812_02505"
     CDS             complement(14432..15892)
                     /locus_tag="PK812_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68452.1"
                     /translation="MTQILDIARGAYAKLPYSARSQIGKVLQFVPARHRYGATHQLWR
                     DRLVRTRDDAATLKALQDEARVSVVRAAYEKSRWYRSALTRAFGASFDPASVLKHDMW
                     SQVPLLTPDTVTRERDALCTRPVDDLDRGNTGGTSGQPVQFYLDRNRSPIEYAFVFDA
                     WARAGYRAGDWRAVFRGVEVQDASRIAIERDPALAEVRFSIFNLTDEMMRRFHSTILR
                     DGIRYLHGYPSAIGMFASFLQRAGLAPMPQIKGVFLMGERLYPNYRNAIEAAFPHASL
                     VPFFGLSEKCAFAVEVAGLPDVYDFEPLYGYTELLDRNDQPVTQPGARGRLVSTGLLF
                     KGMPFLRYDTRDEATLVASPSAANGWKLRVERIVPRRGHEFLVSRDNRLIPILALVVF
                     GDEMLAIREFQFVQDRPGEALLKVVRAAGASHAAAQAYIDLIRRKTGGLLTLSAEYVD
                     ELPPSPRAKRRFVDQRLDIAAVERSMNLSADATAAQ"
     gene            16063..19014
                     /locus_tag="PK812_02510"
     CDS             16063..19014
                     /locus_tag="PK812_02510"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF010706.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HRJ68453.1"
                     /translation="MNLHADASRSSVETLAQRLRSGPRFTRTALKHVDDLCQEDSEGA
                     HRLAQILSRSPAARDLLAGIRDGAGFLWRLILQDTDRLASLLEDDPQHHIERICAIAR
                     ATAELSSEPDAMRRLRRLRAEAALLVALCDLGDVWQVDRVTACLSDFADACVQGAFGF
                     VLNKAITDGQIAPARTSHPTEGLGMFILALGKHGARELNYSSDIDLTVFFDPEAAPFK
                     PAADPQGLSVRMIKGLVRLLQDRNADGYVHRVDLRLRPDPGSTPIVMPVVAALGYYET
                     VGQNWERAAMIKARPIAGDIAAGQAFLKELTPFIWRKYFDYAAISDIHAMKRQIYAVR
                     GHEKVAIEGHDLKVGRGGIREIEFFVQTQQLVYGGRRPALRGARTLDMLSVLRADGWI
                     TERAEAELSEAYRFLRALEHRVQMVGDEQTQRLPSSEEDLKRFAAFCGVTRAAFDKAL
                     TKRLLTVEAHYARLFESMPGLASEAGSLVFTGVDDDPETLETLRKLGFTRPELAAETV
                     RGWHFGRRTAVTTQRAREILTELVPALLEAFGNSTDPDAALLAFDTALGKLPAAVELF
                     AILKNHPGVRSLFAEILGSAPRLAEIVSRRPHVLDSIVDPDFTRPHDADEIERRAEAQ
                     LARERDFEHFLEHARTIAHHEQFLVATQVVSRVLPAEDAGRIHAAIAEAFLRLVLARV
                     EAALAERHGRVPGAEVCILGYGKLGSREMTATSDLDLVVVYNAAPDAVSDGEKPLYAS
                     EYYARLTQRLLTALSAPLRTGTLFEIDLRLRPSGRKGPVAVSLDAFRTYQNGEAETWE
                     HMALCRARPVAGSAAFGAVIASSIQSILAKPRDTRTVARDVARMRALIEQEKPASNAF
                     DVKMMPGGLVDLEFIAQYLVLTHASTDANLIGMPTIVVFENAVAQGWLASDDAETLIE
                     AGRLYTRISQFLRIVLVNDGDVDGQPASFRVKLAQATGLPSYKVLTAEKARLAKEVRR
                     IFVRVVGPVKA"
     gene            complement(19160..19660)
                     /locus_tag="PK812_02515"
     CDS             complement(19160..19660)
                     /locus_tag="PK812_02515"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HRJ68454.1"
                     /translation="MTDLQITIQHEVPGDTAAIENLHDRAFGPGRYARTAERLREAAP
                     HDLRLSFVARVATLLVGSVRLTPIRVGDHPALLLGPLAVEPAFTSRGIGRALIERATK
                     DAAALGYNLVLLVGDEPYYARVGFRRVPPGQMVMPGPVDPARVLVWAADPERCKSIRG
                     QVRAAA"
     gene            complement(19723..20073)
                     /locus_tag="PK812_02520"
     CDS             complement(19723..20073)
                     /locus_tag="PK812_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HRJ68455.1"
                     /translation="MTAGTPEITFADFLKVDIRVGTIVGVEPFPQARKPAFKLRIDFG
                     PEIGIRKSSAQITRHYTPDTLLGRQVAAVVNFPPRQIGPFMSEVLTLGFPDEGGEVVL
                     VQPSIAVPDGGRLF"
     gene            complement(20070..20777)
                     /locus_tag="PK812_02525"
     CDS             complement(20070..20777)
                     /locus_tag="PK812_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRJ68456.1"
                     /translation="MPRTSESLDKRRAAVEALMALAAEKPFDSITLGEIAGKAGLSLV
                     DLRDLFPSKGAILGGFLRIIDREVLQAGGQDMSGEPAHDRVLDVMMRRFDALAPYRDG
                     LRSVRDGLKRDPATAFAFNQAAVNSWRYLLESAGIDTNGSLGLLKMQGAVLVFARAFD
                     TWLTDDPDLSRTMATLDRELKRGETLLGQAEALHRLAAPFRGFARALCQRSRERSDEW
                     RRRGSARAAGDQAEAAT"
     gene            complement(20850..21671)
                     /gene="proC"
                     /locus_tag="PK812_02530"
     CDS             complement(20850..21671)
                     /gene="proC"
                     /locus_tag="PK812_02530"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HRJ68457.1"
                     /translation="MTNWPRSLVLVGAGKMGGAMLEGWLTAGMPGPSIAILDPHLADS
                     MIALAGDSGVRVNPVPSLVADPEVIVLAIKPQMLDAAAEVLGKIAGPQTLVVSVLAGK
                     TIANLRSRMPRARAFIRAMPNTPAAVGRGITGVSACVEVTEAQRRLADRLLSAIGRVE
                     WVPSEDLIDAVTAVSGSGPAYVFYLVECLTEAGIVAGLPAATAARLARATIEGAGELL
                     FQDAATSPGQLRINVTSPGGTTAAALEVLMAENGLKPLMARAVAAAQRRAGELSG"
     gene            complement(21668..22168)
                     /locus_tag="PK812_02535"
     CDS             complement(21668..22168)
                     /locus_tag="PK812_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HRJ68458.1"
                     /translation="MSLTDVAQDRNEHPVDMVERVASLNQWAFERSDEDEISISVGGG
                     WSDYHVSFTWLEDMDSLHMGAAFDLKVPDGRRAEVLKLISFINEQLWVGHFDLWHSEN
                     AVMFRHALLLPGGIVATAEQCESMLKIAVDACERYFQAFQFVVWAGKGAREALSGAMF
                     DTVGEA"
     gene            complement(22482..22736)
                     /locus_tag="PK812_02540"
     CDS             complement(22482..22736)
                     /locus_tag="PK812_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HRJ68459.1"
                     /translation="MSGSNPLLDGISRLMTDAVGVAQGVKKEADSVVRSQIERILRDI
                     DVVTREEFDAVKDMAALARDENEKLAERIAALEEMLASRS"
     gene            22847..23689
                     /gene="lgt"
                     /locus_tag="PK812_02545"
     CDS             22847..23689
                     /gene="lgt"
                     /locus_tag="PK812_02545"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRJ68460.1"
                     /translation="MNPPLTAAFTFPGFDPVAIWIGPLPIRWYALAYIGGLLIGWWYA
                     RSLAAKDRLWGGTKRPAATDLDDLLVYAALGVVIGGRLGYVIFYNPGHFLANPLDTLQ
                     VWRGGMAFHGGLIGAAAAVWLFARRRGLEPLALFDLCCTVVPVGLLLGRIANFINGEL
                     WGRETSVSWGVIFPHAGPVPRHPSQLYEAALEGALLLIVLGWAARRFGFARPGLLTGL
                     FAIGYGLARIASEFFREADEEWGYFAGDWLTMGMLLSLPMILAGAVFLRRALMRRATG
                     RANA"
     gene            23686..24792
                     /locus_tag="PK812_02550"
     CDS             23686..24792
                     /locus_tag="PK812_02550"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRJ68461.1"
                     /translation="MTPLAAELRRLILLEGPMPLDRYMAYCLAHPKHGYYTTRDPLGD
                     AGDFTTAPEVSQMFGEMIGVWAMSVWAQMGRPDAFALVELGPGRGTLMADLLRASKIL
                     PGFRDAAKVHLVEISPVLMERQGHLLAGSGQAVTWVPSLARIDHGLPLIVIANEFFDA
                     LPVRQFVHCGGSWRERLVGLGQDDVLRYGLSPDPVPHMDMPGQEGDIREFCPDGQDIM
                     RQMAERLARDGGAALAIDYGYARSQAGDSLQALRGHAFADPLEQPGEADLTTHVDFAA
                     LSAAAIAGGARAFPLLSQKTLLERLGIGLRAQKLAAAAPDRAADVASALHRLTGTGEG
                     QMGTLFKALAIASSTLGAPPAFDSPDPVLQHLAR"
     gene            24798..25571
                     /gene="pgeF"
                     /locus_tag="PK812_02555"
     CDS             24798..25571
                     /gene="pgeF"
                     /locus_tag="PK812_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HRJ68462.1"
                     /translation="MLDPILSSDLSALQGVRHAYFTRAGGVSNGVYATLNGGIGSDDE
                     AARVQENRGRMADWMGVERSHLVSLWQHHSADVVVVDKPWLQTARPKADAMVTNQPGI
                     ALAIGTADCGPVLFADPQARIVGGAHAGWKGAFTGVLEATVAAMEHLGARRERIVACL
                     GPMISARAYEVGPEFIARFTAAEPGNARYFTPSNKAGHGMFDLPAYISARLVNAGVGR
                     ALDLSICTYGNEDRLFSYRRMTHRGERDYGRQMSVIALV"
     gene            complement(25551..26318)
                     /locus_tag="PK812_02560"
     CDS             complement(25551..26318)
                     /locus_tag="PK812_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009494592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRJ68463.1"
                     /translation="MKAALVTGGALRIGAAIVRRLAASGHAVAIHCNRSRNEAEALAA
                     GINASGGRAMVVCGDLADPATSPRIVAEATHCFGSLTLLVNNAALFEGDSLTVFETDT
                     FSRQIAVNLQAPLLLSRAFAEHACEGGAIINLIDQRVLKPTPYYLSYALTKEALWSAT
                     RILARSLAPRLRVNAVAPGPCLPNSADGPEVFAREVAAIPLQRAVSPDEIAEAVVFLA
                     QAEGVTGQMIAVDGGQHLGWKTPDVLALEGASNQGDH"
     gene            complement(26315..27121)
                     /locus_tag="PK812_02565"
     CDS             complement(26315..27121)
                     /locus_tag="PK812_02565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRJ68464.1"
                     /translation="MSGGPVPDLSIFIITKNEADRLPRTLAAVRNLSDDIVVVDSGSS
                     DDTCALAIASGARVVENEWPGYGPQKRFAEEQCHGTWLLNIDADEVVPPELGNEIRAA
                     VEFPFADAYEIRIAEIFPGERRPHSLGYALAPVRLYRRDKGRYSPSPVHDRVDLAPDA
                     RVARLKGVVHHFSVRSIGDQIAKLNAYTDQQVDDLAARGKHVPVWRLFVEFPASFLKA
                     YIGRRHFARGLYGVITATNFAFYRWLRVAKDVERRLLERDAARQKKEGNS"
     gene            complement(27316..27528)
                     /locus_tag="PK812_02570"
     CDS             complement(27316..27528)
                     /locus_tag="PK812_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HRJ68465.1"
                     /translation="MAQQGTVKFFNTDKGYGFIKPDGGGADIFVHVTAVQRAGLKTLL
                     EGQKISFEVEPDKKGKGPKAINLVAL"
     gene            complement(27699..28544)
                     /gene="rlmJ"
                     /locus_tag="PK812_02575"
     CDS             complement(27699..28544)
                     /gene="rlmJ"
                     /locus_tag="PK812_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HRJ68466.1"
                     /translation="MNYRHAFHAGNFADVMKHAVLARILSYLALKPAPFRVIDTHAGC
                     GAYDLSGHAPNRTGEWREGIGRILGASLPADVEPVLWPYLSVVAPMMREKRPIYPGSP
                     IIAQALMRTQDRLICAEKHPEDARKLVAALAGDRRVKAIEIDGWVALNAQLPPKENRG
                     LILIDPPFEEPGEFGRLARAFLVAYRKWHSGIFALWYPIKDIGAVESFVADLHASNIR
                     KILRLEMWVDQPDSDGPLAGSGMIIVNPPWTLKAECERLLAWLAQATARTARSGCQVV
                     WITPE"
     gene            complement(28554..29213)
                     /locus_tag="PK812_02580"
     CDS             complement(28554..29213)
                     /locus_tag="PK812_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012452789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T2"
                     /protein_id="HRJ68467.1"
                     /translation="MIPRFLFALCLAALATLPAAGQGRDRRGGVPGQFDFYVLALTWS
                     PAFCQAEGARRVSDQCGLKAQDAFVVHGLWPQYDRGFPTECGFGRNPTRSDMDKAMQV
                     FPDEGLARYEWRKHGTCAGGSPADYFEEVTVARGKVTIPEEFTRPVRAFSTTPLEVER
                     AFVAANRGLRSDMMSVQCNRNVLREVRICFTRDLRSFVTCPEVNRSGCRARDITVPVG
                     R"
BASE COUNT         5580 a         9392 c         9092 g         5285 t
ORIGIN      
        1 ggcggcgcag gctcagccag tccgctacga ggtgcagaac ccggcgccgg tgcaacctgc
       61 tggcaagtcc gaagagtatt tcgctacgaa gagcatggtc ttcggcgcca tgatcgaagc
      121 catcgatctg tcccagctct ccaaactgga tgcggaggcc gcgcgcgaag aactgcgcga
      181 catcgtcacc gagatcatca atctcaagaa cgtcgtgctg tcgatcgccg agcaggagga
      241 actgctcgag gacatctgca atgacgtgct ggggtacgga ccgctggagc cgctgctggc
      301 gcgcgatgac atcgccgaca tcatggtcaa cggggcaaac cgcaccttca tcgaaactgg
      361 tggcaaaatc caactcacca atgtccgttt ccgcgacaac gcccagctga tgaatatctg
      421 ccagcgcatc gtcagccagg tcggccgccg cgttgatgaa tcctcgccga tctgcgacgc
      481 gcgcctgctc gacggttctc gcgtcaacgt catcgcgcct ccgctggcca tcgacggacc
      541 ggcactgacg atccgcaagt tcaagaagga caagctcacg ctcgacaacc tggtgaagtt
      601 cggatcgatc tcggcggagg gcgccgacat cctgaagatc atcgggcgcg tgcgctgcaa
      661 tgtcgtcatt tccggaggca ccggttccgg caagacgacg ctgctgaact gcctgacccg
      721 ctacatcgac cacgacgagc gcgtcatcac ctgcgaggac gcggccgaac tgcaactcca
      781 gcagccgcac gtggtgcgcc tggaaacgcg tccgccgaac ctcgaaggca atggccaggt
      841 cacgatgcgc gacttggtca aaaactgcct gcgtatgcgc ccggaacgaa tcatcgtcgg
      901 cgaagtccgc ggacccgagg ccttcgacct gctgcaggcc atgaacacgg gccacgacgg
      961 atcgatgggc acgcttcacg ccaacaaccc gcgcgaatgc ctgagccgtc tggaatcgat
     1021 gatcacgatg ggcggattta ccctgcccat caagacgatc cgcgagatga tctgcgcttc
     1081 cgtggacgtc atcgtgcagg cgacccgcct gcgtgacggc tcgcggcgca tcacgcacat
     1141 cactgaagtg atggggatgg agggtgatgt gatcatcacc caggacatca tcctctacaa
     1201 catcgagggt gaggacgcga acggcaagat catcgggcgg catgcctcga ctggaattgg
     1261 acgtccgcgg ttctgggagc gcgcccgata ctatggcgag accgagcgtc ttgctgccgc
     1321 cctcgacgcc ttgagcagca agcctggcga aagccaggcc gcgtaggaga gacggccatg
     1381 gaaacccgtc aactcctgat catggcgttc gcggccgtcg cggccggcgg gtttgcctat
     1441 gcgttcatcg aaccgcgcat gaccggcgag gtgactgccg aggcgcgcca gaagaagctt
     1501 gccggagcac gtgtgcgcgg aggcgacaac ctcggcaaga ccgccgggcg tatcaagcag
     1561 gtctcggaaa ccttgaaaga ggtcgagcgc aagaaggcag cgaacgacaa ggtcaccctg
     1621 catgacaaga tcgtgcaagc cggtctcgac tgggaacgcc agcggttcat gttggtgagc
     1681 atggcatgcg ctgtggtcat gggcttcgtt tccctgatca tcagcggcaa cctgttcgtc
     1741 ggaatcggcg ccttcatcgt cggcggcctt ggactgccgc aatggctcct gatctttctg
     1801 cgcaacaagc gcgtctcggc gttcaccaca gaacttccga acgcgcttga cgtgatcgtg
     1861 cgcggcctgc gctccggcct gccgctcggc gactgcatcc gcatcatcgc taatgaagcg
     1921 gccgagccgg tcaagaccga attccggcag atcgtcgagg cgcagagtat tggccttacg
     1981 atccaggaag cggttcaggg aatttccaag cgggttccca cgcctgaggc aaacttcttt
     2041 tccatcgtga tcgaaatcca gtccaaggcc ggcggcaacc tttccgaggt tctcagcaac
     2101 ctttccaagg tcctgcgcga gcgcaagaag atgaagggca aggtcgcagc gatgagcatg
     2161 gaggcgaaag cctccgccgc catcatcgcg gcgctgccgt tcatcgtggc gatcctcgtc
     2221 tacatctcca gtccggacta catgtctcgc ctgtggacta cggaagccgg caaaatcacc
     2281 atggtggtga gtggattctg gatgctcatc ggcatcatgt cgatgaagaa gatgatcaac
     2341 ttcgatcttt gaggcgcctg atccatggaa gggaatatcg tcagcctgat cctcagcccc
     2401 agattcatgg gcggggtgtt cacggcgatt ttcgtcggcg cggcaatcct gacagtggcc
     2461 cttccgctgc tcgatcgcag tgacttcaag aaccggatga atgccgtcgc gctggaacgg
     2521 gaagcgatcc gccaacgcga acgggagcgc atgctgcaag tgcgcgggac aggcagaggc
     2581 aacctcaaac acgagcacaa ggcttttgtg aaaggtttcg tcgagaaatt cgacctgcag
     2641 aggtacatcg gcgccgatga aatgaaggcc aagctggcca tggccggcta ccgcggcgcg
     2701 ccggccgaat acggattcat gttcttccgt ttcatcatgc cgatcgtgct gctgctcatc
     2761 acggcgctct acgtcttcgt catcctggac ctctccaact acacggtacc gatgaagatc
     2821 ggcatgtgcc ttgcgggcac atacctgggc ttcaaggcgc cggagatctt cctgtccaat
     2881 cagatctcaa aacgccaaca gaacatgcaa cgggcatttc ccgattccct cgacctgctg
     2941 ctgatctgcg tggaagccgg catgtcgatc gagcatgcgt tccgtcgcgt gagcgtggag
     3001 attgttggcc agtctgtggc tctggccgaa gaatacgcac tggtgacggc cgaactctcc
     3061 tacctgcccg atcgccgcac ggcctacgag aaccttgcca tccgcaccgg catggaatcg
     3121 atcaagtcgg tcagcacggc gctggtgcag gcagagaagt acggcacacc ggttggcagc
     3181 gcgctacgcg tcctggccca ggagggccgc gacatgcgca tgaacgaagc agaaaagaag
     3241 gcagccgcac tgcccccgaa actgacggtg ccgatgatcc ttttctttct tcccgtcctg
     3301 ttcgccgtga tcctgacacc ggccctgacg caggtctttg gctggcagtg atctgcggtc
     3361 ggagaccccc tgaaacagcc tgtgcgactg cctcggacag gcgagacagc ttcactcttg
     3421 caggcacgcc catagacgac gcggtgtagc catgaatgtg cgtttcgtcg gctacttcct
     3481 cgctggagca ttgccgaaac tggccatgtt cctcgcgttg tttctgctgg ccagacatct
     3541 tgatcccgcc gagaccgggc tttttgtcct cgtcatcact cttggcgaag tgatcgagat
     3601 gacagcggga acatggctgc gcatctatgt cctgaaggct gaagcaggcc gcaagaccat
     3661 tggcccatac cgcgtcggtc ggtttctcgt ccttgcgctg gccatgctcg ccatggcgct
     3721 gctggtgtcg ctgccgatcg cggctatcat cgcgccgggc cgattctgga cctttgcagg
     3781 cgctacttgc gcctatatcg ccgcttttgc cgcgctccgc catgcgcata cggtcctcca
     3841 gatcctggag tcgcatgtgg tcttcattct cgcagagatc gcgcgcagcc tgctcatcgt
     3901 cgcctgcgtt ctgggcagcc ttgcgacatg gccgacatct ttcgtggctc cctcgctcct
     3961 ggtaagcgcc gcgaccgccg ctactgccgt gatcagtctg ctcctggttg cctcacgtct
     4021 ccacggtcct cgtttcacac tggccggcat tcagcctgcc gtgatcttcg gtgcacccat
     4081 catcgccgac gttgtcatga gcctggtcat catcaacttc gaccgcttcg tgctcaatca
     4141 aatgatcggc ccggccgccg ttggcatcta tgcgctcgcc tatgcgctcg ggcggcaacc
     4201 gatcgagttc atcgccggtc cgctcaacac ttatgcgctt cctgttctgt ttgccggcta
     4261 cgaacatggc ggagaagagc gtgcacgcca gatcctgacg ggcctgtgca tcaccctgtt
     4321 catactttgc gctgcggtcc tcacgggcat aaccctgctg gagcagccca tagccaacgt
     4381 ggcgctcaga atggagtacc gcgatgcggc gggactgctc atgccggtga tcacgtttgc
     4441 agcctgcctg ctcgtcttca aggcgttcat tttcgacaat gtcttccata tgctgcagaa
     4501 gaacgacgag aagctggctt cggtcgcgct cgcaaccggc gtgggcgtcg cggccaccat
     4561 tctgctggtc tacgttgccg ggatcaacgg ggcagccgcc gcgctggtgc tctccagcgg
     4621 cgtgggactt gctgcaagcg tgtatgtcag ccggcgcttc ttcgtgttcg ccattccgct
     4681 gcgacacttc gcgatgattg cgctcgcggc ctctggctcg ggcgtggcgc tttcgctggc
     4741 atcatgggca gtgtctgcct acggcgatgg cgctcgcata ggcgccggat tccttgcgtt
     4801 ctgtctctgc tacgcgctga tgctgaaact tcagggcatt tctctcaaag ccttggcgtc
     4861 gacgccctgg gagccgttcg atgcccgaac aggcagcgag cgacggccat cggacagcaa
     4921 cttgcggtaa agcgtgacct tggcctccag cgtcttgcgg atgtcgtagc gcgctgcggt
     4981 acgcgcgccg gcggccgcga atttcgcccc gagatccggc gtggcgatca gatgctgcag
     5041 cttgtccgcg aggtcgcgcg ggttaccgac ctcgaagagc aaaccgttgt caccatgccg
     5101 gacgatgtca cgtgcccctc cgacagaagg aacgaccacg ggaagatttg cggccatcgc
     5161 ttcgatgatg gcgatgccga aaccctcctg ccgggacgac agaacgaaga tatccatgtc
     5221 ggacaagaga ttttcgacat ccgtgcgatc cagctgaatc cttacgtgat tctccagatc
     5281 gagttccttg atcagggacg tgaccatttc aaagtaggct gggtccgtca cggatcccgc
     5341 cagtgtacag tcaatgttca gacctcggtc cagacacagg cgaaccgcgc gcaagagcac
     5401 gtcctggccc ttgacaggcg caaagcggcc aacctggacg aggtgaacag gccgccccgg
     5461 cgtttggtaa gcaggcgaac ggataaactt tcggagatcg attccattct cgatcagcgt
     5521 cacgcgatcg cctgtaatgg cgcgcgtttc ctcaacgatg aatggggaga tcgcaacggt
     5581 catgtcgacc atcgcccgat aggccaaacg agagaggccg cgaatgcgaa gcgccccacc
     5641 ctgctcatgc tgggtgtaga caagcttcag gcgcggccgt gccagcttgg cgagcatccc
     5701 ccagaagagg ctgccgtcgt tatgggcatg gattgcttcg atatcatgcg tccggatcac
     5761 acgcaggatc gcggccaggt atctcggatg gagatgccct tcgcgtcgcg ccaggtgata
     5821 gacaggaacg ccgctccctc tcagttccgc catcaggcca ggatcaatcg cctcgttcat
     5881 gacgatcacg acaggccgga tggctgcacc gtccgctgca gccgtcatgg tcgagacgag
     5941 aagccgttcg gcgccgccaa tcctgagcga cgaaatgagg tggataacgc ccggtgtgcg
     6001 cgcttgagtg cccctcgcca tggtcaagcc gccttttcga tgcggcccgc cagacgaagc
     6061 atcatactcc gcgtcagcat cactgcgaag atggcattgg ttcgtgcata gcgccagaac
     6121 atgcgtcgcg gctcctgcat catacgatac gcccactcta gtccaaggcg ctgccagatg
     6181 accggggcgc gtcgcacatg tcccgaaacc acatcgaacg taccgcccac acccatgacg
     6241 aacggcgccc ccatgcgggc acgccaggcc ttcatgaagc gctcctttcg cggtgtcggc
     6301 atggcgacga acacgcagtc agcgccgctc tcccggatcg catccgcaat cgcggcatcg
     6361 tcttccgtct tgaagtagcc atgatgacag ccggcaaacc gcagatcggg atgacgtcgg
     6421 cgaagctcgg cctgcgtagc ggtaaggacc tcctgcgtcg cacccaacag aaacggtcgg
     6481 aagccttcgc gggcgcagag gtccataagg tcctcaaaca gatcgacgcc ggcgacacgt
     6541 tcgtccaccc ggtgccccag caggcgcaac gcatagacga tgcccatgcc atcgatgcca
     6601 accacatcgg cggtcgcgac atcctgccgg agttcggcat ccgtgcgcgc gttcaccagc
     6661 ttcgcgacat tcagcgccac gtgctggcac ggcaccttcg tgcgcatggc tgcgacggca
     6721 cgatcgattg tctcctgccg ggacaggatg tccactggcg tgtcgaacag aaaaacccgc
     6781 attgcttgcc tccgttccga attggtgcgg gcggctcgat gccgccgctc tctgcaggca
     6841 tcgagcaagg atcaggccat ggcgcgcagc gcgcggacat tgcgttcaag cgtgatgaag
     6901 gcccagatct tgtcggcgaa atccgccttg ccggacatgt gcgcggcgac gatcttctcc
     6961 atcgcgccct tcctgaggaa gtcacgttgc cagcttgtcg ccagcgtctc ctcgaccagc
     7021 gggcgcaact cgttgcgcag ccagcggccg agcggcatgt cgaagcccac ctttcgccgc
     7081 gtaacgcact ccgccggcaa atacttcagc gcgagttggc gcaggaggta cttcttgtga
     7141 cgccccttga tgatcatgtg ctctggcagg gatgcggcga agtcagcgac atagcgcgtg
     7201 atgaagggca cgcgcgcctc gatcgaggca tgcattgtcg aacggtcggt gacggtcaga
     7261 aggtcgtctg cgaggcggaa ctgctggtcc agacgcagcg cgtccttgag gccgtgcgcg
     7321 acgggatagc cgactttctt ggctgcgaag acctcaccca gcatgtgcgg ttcgaagatc
     7381 atcgacgtgc cgtgatattc cggcgtgccg gtgacctgga gcagatgatg taccaggcga
     7441 ctcttcggcc agagcaggtc cttcgccaaa gtgccgacga aaggcgcgaa gcggccgaaa
     7501 gcgttcaact gccttgccag cttgaagcga aaatactggt gatagccgcc gaagaactcg
     7561 tcggcgccct cgccggacag cacgaccttg aagcctgact cccgcgcaaa ctgcgccacg
     7621 agatagcgca tcaccgaagc cggttcggcg gaggcatcgt cggcgatgga gggccagtcc
     7681 tccatcatgc cgacgagatc atcgccatcg acgaacttca cgaagtgccg caggccgtac
     7741 ttgcgcgcga tcgcctgtga ataggggctc tcgtcgaagc gctgatcttt gaaaccaatg
     7801 gaaaaacagg ccagatccgg cacatgccgg gcggcgatag ccgtcagcaa tccagaatcg
     7861 atgccgccgg acaggaggct cgcgaccgga acgtcggaaa caagatgccg gcgcacggaa
     7921 aagtgcagca gttgatcgag ctcgtcgagc agctcggcct cgctcttgcg ggaaaccgca
     7981 accggttcgc gcttcaacca gcgcgtcagt tccagcgtgt tggcatcaat gtcgtagacc
     8041 gcgacatgac ccggcggcac ctcatgcacg tcgtcgaaca gcgtgttggc cccgcgcgcg
     8101 gcgcgcgcca tgtagaactg aaggtagcct tctgcattga tgcgcttctc ggaaaaggcc
     8161 atcagcgccg gaatagtcga ggagaaggcg aagacatcgc gcttcgccac gaaataggcc
     8221 ggcttgatgc cgaaggcatc gcgggcgaga taaacctttc gctgcgcctt gttgtaatag
     8281 gcgaaggcga acatgccgtc ggcgcggttg aggaagtcca tgccctgggt gttcaaaccc
     8341 ttgagcagaa cctccgtgtc cgaacgcgtc ctgtagagcg tgttgggctg gcgcaggtca
     8401 gcgtggttgt agatttcgcc gttgaaggcg atggcgccgg cctcgtcctc catgggttgg
     8461 cgaccgccct cgacatcgat gatcgaaagc cgagtgtgga tcatttcgac cggtccgatg
     8521 actgcgctct ccgccgcatc cggtccgcga cgggccaggg acttgagcgc caatgcgcgg
     8581 aagcgcgcgg cagctgtttc atcctgggtg cgctgaacga gtccggcaaa tccgcacatg
     8641 acactggtcc tcaaaaccta ttgaaggggt tcgcagcctt gccgtcgtca ccgcgagcgg
     8701 cgcgaaacaa tccgaccgga actgatgctc aggcttgccg gatcagccca agcctgatcg
     8761 cggtgaagcc atagccggtc actcgtcatc ctgctgctgg agcaggccga cggtgtcgat
     8821 cacaactttg ctcgcgagcg cagcccgatc cgcattgcgg aacttcgaat gcgcgaccag
     8881 gatcgccact acatcggccg cagcaatcgc cgcatggtgg tccatgaatt ctacgccagg
     8941 gcgactggcg aggctcttcg gcagcttttc gatgttcggc tcaacgatca gcagtctgcc
     9001 aatgccgcgc tggctgagaa tctcggcgat ttccagcgcc gggctctcgc gcaggtcgtc
     9061 gacgttcggc ttgtaggaca ggccgaggag cgccacgacc ggttcgcggt agcggttggc
     9121 gaggcgggag atctggtcgg cgacgcgctt gggcttggcg tcgttgacct cgcgggcgtt
     9181 gcggatcagc ggcgtcgcct ccggcagcgc ctcatggatg aaccaaggat cgacgggaat
     9241 gcaatggccg ccgacgcccg gccccggcga caggatgttc acgcgcggat gcaggttcgc
     9301 catcttgatc accgaccaga catcgacgcc caagtgatcg cataccagcg agagctcgtt
     9361 ggcgaaggcg atgttgacat cgcggaaggc gttctcggtc aacttcgaca gttcggcgac
     9421 gcgcgaggtc gtcagcttga tcttgcccat ggcgaaatga cggtagacat cgcgggcccg
     9481 cacggcagag cggtggtcga ggccgccgac gatgcgggcg ttgtcgacca attcgcgcag
     9541 ggtctggccc ggaaggatgc gctccggaca ataggccatc atgatgtccg acacttccgg
     9601 catggtgtcg ggataggtca ggtcgggccg caggcgccgc aactgttccg ccgcgcgttc
     9661 cgtggtgcca accggtgacg tggattcgat gacgacgaga tcccccttct tcagcaccgg
     9721 agcgatcgag gcgagagcag actccaccat gctcatgtcc gcggtcttct cgtcggtgat
     9781 cggtgtcggc acggcgatga gaaagacatc ggccggctgt gcctcggtgt aggccttcag
     9841 catgccggac ttcaccgcgg cgttgaggac catgtcgaga tccggctcgg agatgtgcgc
     9901 gcgaccttca ttgatgcgct ccactgcggc acgcttaacg tcgacgccca cgacattcag
     9961 cccgcgagag gcgagcagag cagccgtcgg caggccaaca tagccaaggc caacgacggc
    10021 caccgtcgcg atccggctgg aggccgcggg gcgctgggcc tggacacttt cctgacggtt
    10081 caattgcgga gcggacatga acatgcttgg cctcctcgtg agtcccggat tgacccggac
    10141 aacaatcatg ggaaaccgga ttgcaaccgg caggccaatg ccgttcgacg gaaagatgcc
    10201 gctgaaactg aactgatcca gactgattcc gagcatccgg tatagggaat cgcaagctct
    10261 tggagcgaga attggctttg gcgctgtccg gagagggaca accgactacc gcctgtgttc
    10321 cagaatgatc ctccaggtac ttggcctgtg aacgtcgcaa aactgcgctg gtacgcggct
    10381 cgcctcgccg cgatgaaccc ggctgaaatc tcgcatcggt ttggagaact ggcgaagaag
    10441 cgcgcctggc gccgggatga ccggggctgg aacacgtatt cgaatgtcgg cgacgggcgc
    10501 gtcctcgata ttccggaact gcgaaaccgg atcgcgtcgg gctggccggc tgcccgcgcc
    10561 ggcctgagcg tcttccgcga agggcgcctg tgcgggctcg gccgcgaggt tccggcgact
    10621 ctgggggctg ccgtctggtt cacggatccg gtccgcggcg tcaggtggcc gggcgcggaa
    10681 aaatattgct tcgacatcga cgttcgcagc accggcgaga ccatcgggga cgtcaagttc
    10741 gtatgggaaa tcaacagact acagtttctg catccactgg cctgcgccat tgcgacgggt
    10801 acggacgagg cggcgatcgc ccagacgctt gacatgctgg cggactggcg cgccgccaat
    10861 cccccctacc gtggtgtaaa ctggatttcc ggcatcgaac tggcgatgcg ccttgtcagc
    10921 ctgacgcttg tgacagcggc cataccccaa gaccgcatgt cggctggcag ccgcaagctc
    10981 atccgccagc tgaccgccgc ccatggattc tggctccagc gctatccatc gcgacattcg
    11041 tccgccaaca accatctggt ggcggaaggg ctggggctgt ttctggccgc acatctggcg
    11101 ccggacctgc cgcaggcgcg gcattgggag agcgaggggc gctcgattct cgaggccgag
    11161 gcagctctcc agatccttcc cgacggcgtg ggtgccgagc agtcgccgac gtaccaggcc
    11221 ttcaccatgg aaatgctggc cttcgcggct ctgatcgggg agctgtcgca catgccgctc
    11281 gcatccgcgg tctacgaacg tctgcgggcg ggcgcttcct tcctgcgtac gctcatggat
    11341 gaatctggcg gcgttccgca gatcggcgac gatgacgagg gccgggtgat cgcggcacct
    11401 ccggatcgtg aacctcgcta tgtggcgtcc gtcacagccg cggtagccgg ccttcttcgg
    11461 gacgatggac tactgccgcc atcgcgtcag gtgcattggc gtgacgccct tttcaatgtg
    11521 ccggcccgca ccgctcgccc agcttccgga ctcagtcagt tcatggaagg cggctattca
    11581 gttggtcgcg aagaggtcaa cgggcgccgc tgcgtgctca cctttgacca cggaccgctg
    11641 ggctatctct cactggccgc gcatggtcat gctgatgcac tcgcggtctg gctgtccata
    11701 gacgggcagc ccgtaatcgt ggacgcgggg acgttcctgt accactccgg gcgcagcatg
    11761 cgagcccaac tgcgcgccag caatgcccac aacacactga ccgtccaggg cgtctcccag
    11821 agcctgccgt cggccgcgtt ttcctggtcg acaaaggccg caacgcggct cgatgaaaca
    11881 cgaccggggc gctggtggag tctgaccggt tcgcacagtg gctatgagcg gcgtttcgga
    11941 ttgcggcacg tgcggtcggt cacccggacg ccgacgggtt ttgccatcag cgatcagctg
    12001 gaaggcgcgc gcaagcagtt cgacgtcgag atcggcttcc tcgtccatcc cgccattgcc
    12061 gtggtacgag agaatgatcg tttcgtgctg ctctccaacg acacggcgat cgcggaagtc
    12121 gtcacgccgg tgtctctctt cgcgcgcatt gtccgtgacg acagggaaac aggcatgggc
    12181 ctgcattccc ctcgcttcgg cgaactcgtc gttgccccct gcatcgtggc atccggcacg
    12241 atgtcggccg atcccgtcgt gacacggctc aacatcctgg cttgaaccgc ccgatcaggc
    12301 cgtcacgatg ccgcgcaact tggacgcgac cgaacgaaga gcctcaaccg cttcctgcaa
    12361 ctcgcgcgtg gacgtgcgtc ctgagccagc gacgagcacg atgtcgtcaa ctgcgccggc
    12421 aaacgcgagc gccgagttgc cctgctccag cgagccacca tcaatgaaca ccagctcaaa
    12481 gctgttcgcc ggaacgagga cattcgcctg aagctgttgc ggcgaggggc gacgcgcccc
    12541 cccctgcggg ccgcagaccg gaagaatacg cagaccggaa cgttcgtcgg tcaagacggc
    12601 gccggcaaga ctgacgcggc ggtccacgac ttcggcgagt ccggcatgcg gctggtgatc
    12661 aagctgcgcc gacagaccat ttccagaggc atcgccatcg atcatcagga cgcgccaggt
    12721 cgcctgggcg gcagcgatgg caacgttcag cacgatctcc gtggccgacg tcttgcgccc
    12781 gaccgtcaga aacaggacgc tgcgggcgcg atcggggagc gcgtgagcgg caaaggtatc
    12841 cgccacgcgg caggcgccaa gcgagaacgg cgccgcgggg ctttcgatca catgcgccac
    12901 aaccccgcca tccttacggc gtcgcgccgg catctcgccg aggaccggca ggttgctggc
    12961 cagttggaac tgcttccggt cacgcagggt cgagtcgaac tgttcgagca ggagcgccag
    13021 catgaggccg atcatgagac ccgcaaccat cgagcccgcc agaatgagac tcctggaaac
    13081 accggcggac tgatgaggcg gagtggcgga agagataatg cgggcgttga gcgtcgtgag
    13141 atcttcctgt tcgctgagtt cccgggcacg cttgaggaat gagctgtaga ttgtgcgcgt
    13201 cgcttcggcc tgacgctcca attccctaag gcgtaccgag gcctccccca tgtccgagga
    13261 ttgcattttc aactggtcga ggctcttcga cagagactcc tcggaggccc gggcccgctc
    13321 gaactcggcc tgagcggact tcgagatgcg gtttagttca tccagcacca gagaccgcat
    13381 gtcacgcgtc tgggcggcaa gcgcaatcat gtcgggatgc ttcgcgccaa gcttggtgcg
    13441 ggcatcggcc tccaggcgct gcgcttccgc gtacttcagg cgcaactgcg aaaggacacc
    13501 ggcctgcgcc gtgtctggaa gctctccgcg ttcaatggcc tggaccgaaa gcttgcggac
    13561 gccatcgaat ttcgaacgcg cttccgaagt gcgagcgcgg gcgaggacga gttgggaatt
    13621 caactcctgc aactgctgtt cgccaacgag gcgaccattg gcggtctgca ggttgttgcg
    13681 ggccttgtat tcctcgacct cgcgttccgc cttctgcaca cgcacagcca gttcttcaag
    13741 ccgcgacgtg acggcatgcg acgctttctt tgcggtatcg ttttccgcgc tggtctggct
    13801 ctcaagaaac acagccgcga cggcgttggc gatgcgcgcc gacttcactg ggtcctgtgt
    13861 ccagaccgtc gcttccacca cagaggagcg atcggcgcga cgcgcgccaa cccgcttctc
    13921 aagggacgcg atcgcgcgca ggcggcgtcc ctccgtcgac tggtcgaatg gcttgccgaa
    13981 ccgcggctgc ttgcctgtga actcctcgtc atcgaacagc ctttcccggt cgacgacgct
    14041 ggcgatggtc gaagaggagc gaagcatgac gatggcgttc tccgcgacac tgttcacggc
    14101 ttccggaacc tgcgaacgga gatttgcgtc gggccgaaca acctgaattt cacgcggatc
    14161 gaccagcacc tggaccatcg cggtgtagcg cggctgcacc tggagcgcag ccagcaggcc
    14221 aatggccgag aacagcagga aagggacgac aatccagaac ttgcgacggg ccagtccgcg
    14281 aaacagatga cggaagtcga tcgtcagcgt gtcggccggc ggaagcggtg catagccggc
    14341 taatccaggg agcgcggcct gcatggccat cagctggctg ccgcggatgt cgttcttcac
    14401 caggaaggtc atggcgtgtt cctcctgtca ctcactgggc ggcggtcgca tcggcagaaa
    14461 ggttcatcga cctttcgacc gccgcgatgt cgaggcgctg atcgacgaag cgcctcttgg
    14521 cacgcgggct tggaggcagt tcatccacat actcggcgga cagtgtaagc agcccgccgg
    14581 tcttgcggcg gatcaggtcg atgtaggctt gcgctgcggc atgacttgct ccggcagcgc
    14641 gcacgacttt cagcagggct tcgccaggac ggtcctggac aaactggaac tcccggatgg
    14701 caagcatctc gtcaccgaag acaacgagcg caagaatggg gatcagccga ttgtcgcggg
    14761 agacaagaaa ctcgtgaccg cgccgaggca caatacgctc gacccgcagt ttccagccat
    14821 ttgcagcgga gggggaagcg accagcgttg cttcatcgcg cgtatcatag cgcaggaacg
    14881 gcatgccctt gaacagcagg cctgtcgaca cgaggcgtcc gcgtgcccca ggctgtgtca
    14941 ccggctgatc gttccggtcc agaagctctg tgtacccata gagcggctcg aagtcgtaga
    15001 catccggcag gcccgcgacc tccacggcaa aagcgcattt ctcgctcagt ccgaagaagg
    15061 gcacgagcga ggcgtgcggg aaggcggcct cgatggcatt gcggtagttg ggataaagcc
    15121 gctcgcccat caggaatacg cccttgatct gcggcatggg cgccagcccg gcgcgctgga
    15181 ggaacgacgc gaacatgccg atggcagagg gatagccgtg gaggtagcgg ataccatcgc
    15241 gcaggatggt gctgtggaag cgccgcatca tctcgtcggt gagattgaag atcgagaacc
    15301 gcacttccgc cagcgcagga tcacgctcga tggcgatccg ggacgcgtcc tgcacctcaa
    15361 cgccgcgaaa caccgcacgc cagtcgccgg cccggtagcc ggcgcgcgcc caggcgtcga
    15421 acacaaacgc atactcgatc ggagaacgat tgcggtcgag atagaactgc acgggctggc
    15481 ccgacgtgcc gcccgtattg ccgcgatcca ggtcgtcaac agggcgcgtg catagcgcat
    15541 cgcgctcgcg tgtcaccgtg tcgggcgtaa gcaacgggac ctgggaccac atgtcgtgct
    15601 tcaggactga cgccgggtca aaactggcac cgaacgcccg cgtcaacgct gatctatacc
    15661 agcggctctt ctcataggcg gcgcggacaa ccgagacgcg ggcttcgtcc tgcagtgcct
    15721 tgagggtcgc cgcatcgtct cgcgtccgca ccagcctgtc gcgccagagt tgatgcgtgg
    15781 cgccatagcg gtggcgcgcc ggaacaaact gcaacacttt cccgatctgg gaccgcgcgc
    15841 tgtagggcag cttggcgtag gctccacgtg caatgtcaag gatctgtgtc atatcaagac
    15901 gtccgggtct gaatgcgctg tcgtgccaga ccttgagcaa gcaatgtgcc gccagagcat
    15961 accgatccgg cgcggccatg cgacgcaacc gcgaagggct tgctgcggac ggtcgttcca
    16021 tcttacctgc atcgacatgg gtgacacgta ctctggccgg acatgaacct gcacgctgac
    16081 gcctccagaa gctctgttga aacgctcgcg cagcgcttgc gtagcggccc aagattcacg
    16141 cgcaccgcgt tgaagcatgt cgacgacctc tgtcaggagg acagcgaagg cgcccaccgt
    16201 ctggcgcaga ttctgtcgcg ctctcccgcg gccagggatc tgctggcggg aattcgcgat
    16261 ggcgccggct ttctctggcg cctgatcctg caggatacgg accggctcgc cagcctgctc
    16321 gaagacgatc cacagcacca tatcgaacgg atctgcgcca tcgcccgcgc cacggcggag
    16381 ctttcatcag aaccggacgc gatgcgccgc ctgcgccggc tgcgtgcgga agccgccctg
    16441 ctcgtcgcct tgtgcgatct cggcgacgtc tggcaggttg atcgggtcac cgcctgcctc
    16501 agcgacttcg ccgatgcctg tgtgcagggt gccttcggat tcgtgctcaa caaggccata
    16561 actgacggcc agattgctcc tgcccgaacg agccacccga cagaagggct cggcatgttc
    16621 attctggcgc tcggcaagca tggcgcgcgg gaactgaact attcgtcaga catcgacctg
    16681 acggtgttct tcgatccgga agcggcaccg ttcaagcccg ccgcagatcc gcaaggcctt
    16741 tcggttcgaa tgatcaaggg actggttcgc ctgctgcagg atcgcaacgc cgatggatat
    16801 gtgcatcgcg tcgacctgcg cctcaggccc gatcctggct ccacgcccat cgtcatgcca
    16861 gtggttgccg ctctgggcta ctatgaaacc gtcgggcaga attgggagcg cgcggccatg
    16921 atcaaggccc gccccatcgc cggagacatt gcggccgggc aggctttcct caaggaactg
    16981 acgccgttca tctggcggaa gtatttcgac tatgccgcga tcagcgacat ccacgcgatg
    17041 aagcgacaga tttacgccgt gcgcggtcac gagaaggtcg ctatcgaagg tcacgatttg
    17101 aaagtcgggc gcggaggcat tcgcgagatc gaatttttcg tgcagactca acagcttgtc
    17161 tatggcggtc gtcgtccggc attgcggggc gcgcgcacgc tggacatgct ttcggttctg
    17221 agggcggatg gctggatcac cgaacgcgcc gaggccgaac tgagcgaggc ctatcggttc
    17281 ctgcgcgcac tggagcatcg cgtgcagatg gtcggcgatg agcagacgca gcggctgccg
    17341 tcgagcgagg aggacctcaa gcggttcgcg gccttctgcg gcgtcacacg agcagccttc
    17401 gacaaggcgt tgaccaagcg cctgctgacc gtcgaggctc attatgcaag gctgttcgaa
    17461 tccatgcccg gactcgcctc cgaggccggc agcctggtgt tcacgggcgt cgatgatgat
    17521 ccggaaacgc tcgaaacgct gcgcaagctc ggcttcaccc ggcccgaact ggcggccgaa
    17581 accgtacgcg gctggcattt cggacgccga acggcggtca cgacccagag ggcacgtgaa
    17641 atcctcacgg aactcgtgcc agctttgctg gaggcattcg gcaactcgac cgatcccgac
    17701 gccgcgctgc tcgcgttcga cacggcgctg ggaaagctgc ccgcagcggt ggagcttttc
    17761 gcgatcctca agaaccatcc cggcgttcgc agcctgtttg cggagatcct cggcagcgca
    17821 ccacggcttg ccgagattgt gtcgcggcgg ccgcatgtgc tcgattccat cgtcgatccg
    17881 gatttcaccc gtccgcatga cgccgacgaa atcgagcgcc gggccgaggc gcagctggcg
    17941 cgggaacgcg acttcgagca ctttctcgaa catgctcgga ccatcgccca ccatgaacaa
    18001 ttcctcgtgg ccacccaggt tgtttcccgg gtgctgcctg cagaggatgc agggcgtatc
    18061 catgcggcca tagccgaggc gttcctgcgc cttgtgctgg cccgcgtgga ggctgcactg
    18121 gcggagcgcc acgggcgcgt gcctggcgcg gaagtatgca tcctcggtta cggcaagctc
    18181 gggtcccggg agatgacagc tacatccgat cttgacctcg tggtggtcta caatgcggcc
    18241 cccgatgctg tttcggatgg cgaaaagccg ctttacgcgt cggagtatta cgcccggctc
    18301 acgcagcgcc tcctgacagc cctgtccgcc cccttgcgta ctggaacgct gtttgagatc
    18361 gatctgcggc tgcgcccctc cgggcgcaag gggcccgtcg cggtgtcgct ggacgcattc
    18421 cgtacctacc agaacggcga ggcagagacc tgggagcaca tggctttgtg ccgcgccagg
    18481 ccggtggccg gcagcgcggc cttcggcgcg gtcattgcct cgagcatcca gtccatcctc
    18541 gccaagccgc gcgacacgag gacagttgca agggacgtgg cccgcatgcg tgcgctcatc
    18601 gagcaggaga agccggcctc caacgccttc gacgtcaaga tgatgccggg cggcctcgtc
    18661 gacctcgagt tcatcgccca gtatctcgtt ctgacccatg ccagcactga cgcgaatctt
    18721 atcggcatgc cgaccatcgt cgtgttcgaa aatgcggtgg cacagggctg gctggcgagt
    18781 gacgatgccg agacgttgat cgaagccggg cgcctgtata cgcgcatcag ccagttcctg
    18841 cgcatcgtcc tcgtcaacga tggtgacgtg gacggacagc cggcgagttt ccgcgtgaaa
    18901 ctggcgcagg ccaccggcct gcccagctac aaggtcctga cggccgaaaa ggcccgcctg
    18961 gccaaggaag tccgtcgcat ctttgtgcgg gtcgtcgggc cggtgaaggc ctgatcatcg
    19021 gagccgcgcc ggcaatagcc gccgattcat gccaagggtc atgaccaatc cccttggttc
    19081 ccattccgaa ctttcattgc ccatcatgaa caatgaaggt gcagaagtgg tcctcggtca
    19141 cgtctcgctc ccaatggcat caggccgccg cgcggacctg ccctcgtatc gacttgcagc
    19201 gttccggatc cgcggcccag accagcaccc gcgccggatc gaccggcccc ggcatgacca
    19261 tctggcccgg cggcacgcgc ctgaagccca cgcgcgcgta gtagggctcg tcaccgacca
    19321 gcagcacaag attatagccg agcgcagcgg catccttggt ggcgcgctcg atcaatgccc
    19381 tgccaatgcc cctgcttgtg aaagccggct cgaccgccag cggacccagc agaagcgccg
    19441 gatggtcgcc gacccggatc ggcgtcaacc gcacggaccc gaccaggagc gtcgccacgc
    19501 gcgccacgaa actcaatcgc agatcgtgcg gcgcggcctc gcgcaagcgt tccgccgtac
    19561 gcgcataccg gcctgggcca aaagcccggt catgcaaatt ttcgatagcg gccgtatcgc
    19621 cgggcacctc atgctggatg gtgatctgca ggtccgtcat gggcttttcc gtttgcgttc
    19681 cgatacaacg cgcaatgctt tccgccaagc cagcaagtcg cttcagaaga ggcggccgcc
    19741 atcgggaact gcgatcgagg gctgaacaag cacgacttcg ccgccttcat cgggaaatcc
    19801 aagggtcagc acttccgaca tgaagggccc gatctgacgc ggggggaaat taacgaccgc
    19861 ggcaacctgc cgaccaagga gcgtgtcagg ggtgtagtgc ctggtgatct gggccgatga
    19921 cttgcggatg ccgatctccg gaccgaagtc gatgcgcagc ttgaaggcag gcttgcgcgc
    19981 ctgcgggaag ggttcgactc cgacgatcgt cccaacccgg atatccacct taagaaaatc
    20041 cgcaaacgtg atttcaggcg ttcctgccgt cacgtcgccg cctccgcctg atcaccggcc
    20101 gcgcgggcgg aaccgcgcct gcgccactcg tcactccttt cgcgcgagcg ctgacagagc
    20161 gcgcgggcaa agccgcgaaa cggagccgcg aggcgatgca aggcctcagc ctggcccagg
    20221 agggtttcac cccgtttcaa ctcgcggtcg agcgtcgcca tggtgcgcga caggtccgga
    20281 tcatcggtca gccacgtatc aaaggcccgc gcaaacacca gcacggcccc ctgcatcttc
    20341 agcagcccca gcgatccgtt cgtgtcgatg ccagccgatt ccagcaggta gcgccaggag
    20401 ttgaccgcag cttggttgaa agcgaaagcg gtggcgggat cgcgcttcag gccgtcgcgc
    20461 accgagcgca gaccgtcgcg gtaaggggca agggcatcga agcggcgcat catgacatcc
    20521 agcacgcgat catgggcggg ttcgccagac atgtcctggc cgccggcctg cagcacctca
    20581 cggtctatga tccgcaggaa gccacccaga attgcacctt tcgaggggaa aaggtcgcgc
    20641 aggtccacaa ggctaagccc tgccttgccg gcgatttccc caagcgtgat ggagtcgaat
    20701 ggcttttccg ccgcgagcgc catcagcgct tcaacggccg cacggcgctt gtccaagctc
    20761 tcggatgtac ggggcatggg cttctcctga tcgatcccga atgtagccta cagcgggccg
    20821 gtagcaaggt gcacgagtgc cttgttcgat cagccggaca gttcgcccgc ccggcgctgc
    20881 gcagccgcca ccgcacgggc catcagcggc ttgaggccat tttccgccat cagtacctca
    20941 agcgcagcgg ccgtcgtgcc gcccggagag gttacgttga tgcgcaattg gcccgggctt
    21001 gtcgcggcgt cctggaaaag cagctcgccg gccccctcaa tggtcgcacg cgccaatcga
    21061 gcagccgttg ccgcaggcag tccggccacg atgccggctt ccgtgaggca ctcgaccaaa
    21121 tagaagacat aggcggggcc tgaaccggaa accgccgtaa cggcatcaat caagtcttcg
    21181 ctggggaccc attcgacacg accgatcgcg gagagcagcc tgtccgccag gcggcgctgc
    21241 gcttccgtga cctcgacaca ggcggagacg ccggtgatgc cgcgcccaac cgcagcaggg
    21301 gtgttcggca tggcccggat gaaggcccga gcgcgcggca tgcggctccg caagttggca
    21361 atcgtcttcc cggcgagaac cgagacgacc agcgtttgtg gcccggcgat cttgcccaga
    21421 acctcggcgg cggcatcgag catctgcggt ttgatcgcga ggacgatcac ttctggatcg
    21481 gcaacaaggg acgggaccgg attcaccctt acgccactgt cgccagcaag cgcgatcatc
    21541 gaatccgcga ggtggggatc aaggatggct atggagggcc ccggcatgcc ggcggtcagc
    21601 cagccttcga gcatagcgcc gcccatcttg ccggcgccca ccaggacgag tgaccgaggc
    21661 cagttggtca tgcttcgcca accgtatcga acatggcgcc ggacagcgct tctcgcgcgc
    21721 ccttgccggc ccagactacg aactggaaag cctgaaagta gcgttcgcag gcatcgacag
    21781 cgatcttcag catgctttcg cattgctcgg ccgtcgccac gatgccgccc ggcagcagca
    21841 atgcgtggcg aaacatgacc gcattctccg agtgccacag gtcgaaatgg ccaacccaga
    21901 gctgctcgtt gatgaaagag atcagtttga gcacttcggc acgccgtccg tccggcacct
    21961 tcaggtcaaa agccgcaccc atgtgcagcg aatccatgtc ctcaagccag gtgaagctga
    22021 cgtggtaatc ggaccaccca ccgccaacgg agattgaaat ctcatcttca tccgagcgtt
    22081 cgaaagccca ttggttcaag gaagcaaccc gttcgaccat gtcgaccggg tgttcgttgc
    22141 gatcctgagc gacatctgtg agcgacatgc ggcctcattg cagaacttga gccgggacag
    22201 agccgggaca tcgctgccca gcccaaggtc acatacgcca cgcacctttt ttcgaagacc
    22261 tttcgattaa ccgaagtcct tggcgaaact catgaaatcg cccgaaaaca gcagccgaat
    22321 cagtcttccg tccgacagta gatcgaaaag gcgcctgaat gaatccggat tctcgatcct
    22381 gcagcgcgcc ggtcctgacc tgaccggggc gaacctctgt gactcgcgcc gaagccacaa
    22441 cggccaatgc gcttcgtcca cagattcggc gcgggatcag gtcaggaacg ggaggcgagc
    22501 atttcttcga gcgcagcgat gcgctccgcg agcttctcgt tctcgtcgcg tgccaaggcc
    22561 gccatgtcct tcacggcgtc gaactcttcg cgtgtcacca catcgatgtc gcgcaggatg
    22621 cgctcgatct gggagcgcac cacgctgtcc gcctctttct tgacgccttg cgcgacgccg
    22681 accgcatcgg tcatcagacg ggaaatgcca tcaagaaggg gattggagcc ggacatggtc
    22741 gacctgtctg aatgggctac gaagcgcgct gcatagcgca ccatcgcgga cgtgtcatca
    22801 cgcaggccat tgacgggacg atacgctgcg cgcagatgct ggcaccatga acccgccgct
    22861 gaccgctgcc ttcactttcc caggatttga tcccgtggcg atctggatcg gccctttgcc
    22921 gatccgctgg tacgcgctcg cctatatcgg cggtctgctg atcggctggt ggtatgcccg
    22981 cagcctcgcc gcgaaggatc gcctctgggg gggcacaaaa cgcccggccg ccaccgacct
    23041 ggacgacctc ctcgtctatg ctgccctggg tgtcgtcatc ggtggccgtc tcggctacgt
    23101 tatcttctac aatcccgggc atttcctcgc caaccctctg gacaccctac aggtctggcg
    23161 cggcggcatg gcgtttcatg gcggattgat cggcgcggcg gccgcggtct ggcttttcgc
    23221 cagacggcgc ggactcgaac cgctcgcgct gttcgacctg tgctgcaccg tcgtgcctgt
    23281 cggcctgctg ttgggccgca tcgccaattt catcaacgga gagttgtggg gacgcgagac
    23341 gagcgtctcc tggggcgtga tctttccaca tgccgggccg gtgccccgcc accccagcca
    23401 gctctacgaa gccgctctcg aaggcgctct gctcctcatc gtcttggggt gggcggccag
    23461 acgcttcggc ttcgcgcggc ctggcctgct gaccgggctt ttcgccatcg gctacgggct
    23521 tgcgcggatt gcgtccgagt tctttcgcga agcggacgag gaatggggtt atttcgccgg
    23581 cgactggttg acgatgggca tgctgctgtc acttccgatg atcctggccg gcgcggtctt
    23641 tctcaggcgc gcgctgatgc gccgagccac tggcagggcg aacgcttgac gccgctcgca
    23701 gccgaactcc ggcggctgat cctgctcgaa gggccgatgc cgctcgatcg ctacatggcg
    23761 tactgcctcg cgcatccgaa gcatggctac tacacgacac gcgatccgct gggagacgcc
    23821 ggcgacttca ccacggctcc tgaagtcagc caaatgttcg gcgagatgat cggtgtctgg
    23881 gccatgtcgg tctgggcgca gatggggcgg ccggacgcat tcgcgcttgt cgaacttggt
    23941 ccggggcgcg gcacgctgat ggccgacctc ctgcgcgctt cgaaaattct gccggggttt
    24001 cgtgatgctg caaaggtgca tctcgttgaa atcagtccgg tcctgatgga gcggcaaggg
    24061 cacctcttgg caggtagcgg tcaggccgtc acctgggtgc cgagcctcgc gcggatcgac
    24121 cacgggctgc cgctcattgt cattgcgaat gagttcttcg atgccctgcc cgtgcgccag
    24181 ttcgtgcatt gcggtggcag ctggcgcgaa aggctggttg gcctcggcca ggatgacgtg
    24241 cttcgttatg gcctgtcacc cgatcccgtt ccgcacatgg acatgccggg acaggagggc
    24301 gacattcgtg aattctgtcc ggatggacaa gacatcatgc ggcaaatggc cgagcggctg
    24361 gcgcgcgacg gtggagcggc ccttgccatc gactacggtt acgcaaggag ccaggccggg
    24421 gacagcctgc aagctctgcg ggggcacgca ttcgcagatc cgctggaaca gcctggagaa
    24481 gcggacctga caacacatgt cgatttcgca gcactctctg cggcggccat tgccggtgga
    24541 gcacgtgctt ttccgctgct gagccaaaag acacttctcg aacgcttggg gatcggcctg
    24601 cgcgcccaaa agctggcggc ggctgctccg gaccgcgccg ccgatgtcgc gtccgcgctg
    24661 catagactga caggcaccgg cgaggggcag atgggaaccc ttttcaaggc gctcgccatc
    24721 gccagttcaa cgcttggtgc accgcctgca tttgacagcc ccgacccggt gcttcaacac
    24781 cttgcgcgat gacacagatg cttgatccga tcttgagttc cgacctttcc gcacttcaag
    24841 gcgttcgcca tgcttatttc acgcgcgccg gaggagtgtc gaacggggtc tacgcaacgc
    24901 tcaatggcgg catcggatcg gacgacgagg ccgcccgggt gcaggagaac cgtggccgca
    24961 tggcggactg gatgggcgtc gagcgcagcc atctggtttc gctctggcag caccattccg
    25021 ccgatgtggt ggtcgtggac aaaccctggc tccagacggc gcgtcccaag gccgacgcca
    25081 tggtgaccaa ccagcccggc atcgcactcg ccatcggcac ggcggattgc ggacccgtgc
    25141 tgtttgcgga cccacaagcg cgcatcgtcg gcggtgcgca cgcgggctgg aagggagcgt
    25201 tcaccggtgt tctcgaagcg acggtcgctg cgatggagca cctcggcgca cgccgcgagc
    25261 gcatcgtcgc ctgccttggc cccatgatca gcgctagggc ctatgaagtg gggccggagt
    25321 tcatcgctcg attcacggct gccgaaccgg gaaatgcccg ttacttcacg ccttcgaaca
    25381 aggcaggaca cgggatgttc gatctgcccg cctacatatc tgcacgactc gtgaacgcgg
    25441 gcgtgggccg cgcgcttgac ctgtctattt gtacctatgg caatgaggat cggctgttca
    25501 gctatcggcg catgacgcat cgcggcgaac gcgactacgg tcgacagatg tcagtgatcg
    25561 ccctggtttg aggcaccctc cagcgccagc acgtcgggcg tcttccagcc caggtgctgg
    25621 ccgccatcaa cggcgatcat ctgtccggtt acaccctctg cctgcgccag aaatacaacc
    25681 gcctcggcga tctcgtcggg ggaaacggca cgctgcaagg gaattgccgc cacttcgcgc
    25741 gcaaaaacct ccggaccgtc ggcggaattc ggaaggcaag gccccggcgc gaccgcattg
    25801 acacgcaggc gcggtgccag cgaacgggcc agaatccgcg tcgccgacca gagcgcttcc
    25861 ttcgtcagcg cgtaggaaag atagtacggc gtcggcttga gcacgcgctg gtcgatcagg
    25921 ttgatgatcg cgccaccctc gcacgcatgc tcggcaaaag ccctgctgag cagcaagggc
    25981 gcctgcaaat tgacagcgat ctgccttgaa aacgtgtcgg tttcgaagac cgtcaggctg
    26041 tcaccctcaa agagagcggc gttgttcacc agcaaggtca aggatccaaa gcaatgcgtg
    26101 gcttcggcga cgatacgcgg cgatgtagcc ggatcagcca gatccccgca aacgaccatc
    26161 gcccttccac cggaggcatt gataccggcc gccagcgcct cggcctcatt ccgcgagcga
    26221 ttgcagtgaa tggccacggc atggcctgac gcagccaatc gtcgaacgat cgccgcgccg
    26281 atgcgaagcg cgccacccgt caccaacgct gccttcacga gttcccctct ttcttctgcc
    26341 gagcggcatc gcgttccagc aggcgccgtt ccacgtcctt ggcaacgcgc agccaacgat
    26401 agaaggcaaa gttggttgcc gtgatcactc cgtaaagccc ccgcgcgaag tgccggcggc
    26461 caatatacgc cttcaggaag gaagccggga attctacgaa cagccgccag acaggcacgt
    26521 gcttcccacg cgctgccaga tcgtccacct gctggtcggt gtaggcgtta agcttggcta
    26581 tctgatcgcc aatcgaccgc acggaaaagt ggtggaccac ccctttcagc cgagccacgc
    26641 gagcatcggg cgccaggtca acccgatcat gcaccggcga aggcgaatag cgtcccttgt
    26701 cgcgccggta gagccggacc ggagccagtg catacccaag cgaatggggt cgccgctctc
    26761 caggaaagat ctccgcaatg cggatctcat aagcatcggc aaaaggaaat tcgaccgccg
    26821 ctcgtatctc gttcccaagc tctggtggaa caacctcgtc ggcatcgatg ttcagaagcc
    26881 atgtgccgtg gcactgctcc tcggcaaatc gcttctgggg gccataacca ggccattcgt
    26941 tctcaacgac ccgggcaccc gacgcaatgg ccagagcaca agtgtcgtca ctcgaaccgg
    27001 aatccaccac aacgatgtca tcggagagat tgcgcacagc ggccagcgtg cgcggcaggc
    27061 gatccgcctc gttcttggtg atgatgaaga tcgatagatc gggaacggga ccgccggaca
    27121 tggcggcttc gtggcatgac agcacttgtg gcgcaagcat tgcgagggtt tcacacgatc
    27181 ccctgccgcg acacggcctg cgcacgctaa ccgatcagtc ctggccaccg ggaaaaggaa
    27241 agccgcttca accgggacac aagcaaagcc caccctgcga caccacgagc cgcaacgcag
    27301 gcgggccagc gcgattcaga gcgcgacgag attgatggcc tttgggccct tgcccttctt
    27361 gtccggttca acctcgaacg agatcttctg cccttccaac aaggttttca gacccgcccg
    27421 ctgcaccgcg gtgacatgca caaagatgtc cgcgcccccg ccgtccggct tgatgaagcc
    27481 gtagcctttg tcagtgttga aaaacttcac agttccctgc tgcgccatcg cacgcgcgtc
    27541 ctttccgctt ccctcccgcg cggatcattg ccgcgcccca cgaaaagcca agggaacccg
    27601 tttcgcatcg actgtcccaa gacaccccgc gaaagtggat aatttggcga aaaccgcgac
    27661 ttggcaagcg aaaatcggac tagttgtcga tcctgcgctc actcaggcgt tatccaaacc
    27721 acttggcaac cgctccgagc ggtccgcgcc gtggcttggg cgagccaggc aagcaggcgt
    27781 tcacactccg ccttcaaggt ccaaggcggg ttgacgatga tcatcccgga gccggcaagc
    27841 ggtccgtcag aatccggctg atcgacccac atctcaaggc gcaggatctt gcgaatgttc
    27901 gaggcatgga ggtccgcaac aaaggattcg accgcgccaa tatccttgat cgggtaccac
    27961 agcgcgaaaa taccgctatg ccacttgcgg tacgcaacca agaaggcccg cgccagacga
    28021 ccgaactcgc cgggttcctc gaaaggcgga tcgatcagga tcaggccccg gttttccttc
    28081 ggcgggagct gtgcattgag cgcgacccag ccgtcaatct cgatcgcctt tacgcgtctg
    28141 tcgccagcca acgcggcaac gagtttcctg gcatcctccg ggtgtttctc cgcgcagatc
    28201 aaccgatcct gcgtccgcat caatgcttgt gcgataatcg gagacccggg gtagatcggg
    28261 cgtttctccc gcatcatcgg cgcaacgacc gacaggtagg gccacaacac cggctcgacg
    28321 tcagccggca aggaagcgcc caggatgcgc ccgataccct cacgccattc gccggtccgg
    28381 ttcggcgcat gcccggaaag atcgtaggcg ccgcagccgg catgcgtatc gataacgcgg
    28441 aaaggcgccg gcttgagtgc gagatagctg agaatgcgcg ccagcacggc atgtttcatg
    28501 acatcggcga aattgccggc atgaaaggcg tggcgatagt tcatcgcccc tgatcagcgc
    28561 ccgaccggaa cggtgatgtc gcgcgcgcga cagccgcttc ggttcacttc cggacaggtc
    28621 acgaaggacc tgaggtccct ggtaaagcag atccgcacct cgcgcagcac gttgcggttg
    28681 cactgcaccg acatcatgtc agatcgaagt ccccggttgg cggccacgaa cgcgcgctcg
    28741 acttcaagcg gtgtcgttga gaaggcgcgg acaggacgcg taaactcctc cggaatggtc
    28801 accttgccac gagcgaccgt cacttcctcg aaatagtccg ccgggctacc acccgcacag
    28861 gttccgtgct tgcgccactc gtaccgggcg agcccttcat cggggaaaac ctgcatcgcc
    28921 ttgtccatat cggaccgggt cggattgcgg ccgaagccgc attccgtcgg aaacccacga
    28981 tcgtactgcg gccagagccc atggacgaca aaggcatcct gtgccttcag gccgcactga
    29041 tcggatacgc gccgggcgcc ctcggcctgg cagaatgccg gggaccatgt cagcgcaaga
    29101 acatagaaat cgaattgccc gggcacgccg ccccgccgat cacggccctg ccctgccgcc
    29161 ggcaaggttg cgagcgccgc aagacacagg gcgaacaaga aacgcgggat caaggccgtt
    29221 ctcagggacg tgccatcttg cagtcgggcg cactcgccca attgcgatca taggcctgga
    29281 cgcagaaatg caccccccag gtcatgccga tcatgcccgc gtcctcgaca atagaataga
    29341 agatacggc
//
LOCUS       DAOMXN010000013        25918 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_1199_length_25918_cov_11.599544, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000013 DAOMXN010000000
VERSION     DAOMXN010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 25918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25918
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1199_length_25918_cov_11.599544"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PK812_02585"
     CDS             complement(<1..312)
                     /locus_tag="PK812_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68468.1"
                     /translation="MTIRFTTIVSALYGLMLAFAVVASAASRPLAEAGPLLAGDSFAE
                     TAKAIDIIAASGDPRASDILGALADRRLFVAAGGVVVLRSRTGDLSSALDGAALKVAP
                     DG"
     gene            complement(405..1697)
                     /gene="urtA"
                     /locus_tag="PK812_02590"
     CDS             complement(405..1697)
                     /gene="urtA"
                     /locus_tag="PK812_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HRJ68469.1"
                     /translation="MTLSRRSLLASTIAATAASALPSVPVFAQSGDTIKVGILHSLSG
                     TMAISETTLKDVMLMLIAEQNAKGGLLGKKLEAVVVDPASNWPLFAEKARELITQHKV
                     AATFGCWTSVSRKSVLPVFKELNNILFYPVQYEGEESERNVFYTGAAPNQQAIPAVDY
                     LMSKDGGEAKRWVLAGTDYVYPRTTNKILEAYLLSKGVAKEDIMINYTPFGHSDWQTI
                     VSDIKKFGSAGKKTAVVSTINGDANVPFYKELGNQGIKATDIPVVAFSVGEEELAGID
                     TKPLVGHLAAWNYFQSVKNPANEAFIKQWKAFKKSDKAVTNDPMEAHVIGFAMWVKAV
                     EKVKTTDIDKVIDALPGIEVPNLTGGMSKMLANHHITKPVLIGEVRGDGQFDVVWQTK
                     DLVPGDAWTDHLEGSKDLEADWVGKKCGNFNMKTNKCG"
     gene            1728..2819
                     /locus_tag="PK812_02595"
     CDS             1728..2819
                     /locus_tag="PK812_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68470.1"
                     /translation="MPSIRCAKPTIYLNYNSFFWSDAGICRRIRQAKCQQRGQLHTIR
                     LVAPKFAQGAWFSIKMATVWALAPAERKPVHRKAGRSKERQRLLRSWIRSCPLGMRGA
                     GPAAGRLTDMKHLIIAAMAVVATLQVAMSQVPTGLRLVSDTPRGHLGETISGDAFVET
                     QRRLLMLVTFDKAAAGQPMDVWVRAMKTTASRSALLISGSVKIDAEGRIPLTVSLPRQ
                     WPVGEYVVQVARPGEKPVELPFRVRPESPRSTPLKVSAIELDRIVGAGKSERVQRFLP
                     SDRHWNFGATVEGARTEGVDVRWVLTAVQTTSGSAEVGKIDVPRRPIENTELQFDMEL
                     PRDWPVGRYRVELFLNGKSEAMKEFDVRP"
     gene            2806..2943
                     /locus_tag="PK812_02600"
     CDS             2806..2943
                     /locus_tag="PK812_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68471.1"
                     /translation="MSGRDAAGVADNLVVPDCSNNAGPLGAGLEAACGLLTVEGLVGF
                     S"
     gene            2988..3422
                     /locus_tag="PK812_02605"
     CDS             2988..3422
                     /locus_tag="PK812_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68472.1"
                     /translation="MQYTGIRRWIIALSLAGLASFAPDALAQGASETTRTREGTRVTG
                     TGGNFGGGYDVDRPGRRVTGTGRNFGSGYEVDRSGRRMTGTGRNFGSGYETSRSRQRI
                     TGTGQNFGKGWMRSNNGKEWLGTGANFGKRCPVTATPPTVSC"
     gene            3506..4159
                     /locus_tag="PK812_02610"
     CDS             3506..4159
                     /locus_tag="PK812_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68473.1"
                     /translation="MTRYALAALFVLGHLAPGFAQAPAPSDGTATASYIRAKPEQMLE
                     VYPREVVTRVFSNEFTEARRSKVMSTIQGAPGVNCPERPNVVAYAVIPFKLGKDQTTW
                     IERYAVGCDKAIGRNFLVILEPAGVKLLELVRGATRTDPFLQRDLGGPVGGIAQSRRP
                     QGCQSHIIADTAITQQPPQGGGPWVEKWDIAACDHRSSIEITFTPSAQGGTTWNIKP"
     gene            complement(4221..5300)
                     /locus_tag="PK812_02615"
     CDS             complement(4221..5300)
                     /locus_tag="PK812_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007199016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HRJ68474.1"
                     /translation="MVPDFDPHASPAPALRRTVHGALARLAEARDESLDGALSALARP
                     DCLWRVAHPINSCAGNAEALTRAFAPLKRALPDLERRDVIFVGGTYQRRIYLAAVGHY
                     CGTMRGDWLGIPATNRPIYLRYGEVYEVDAQGAVVQANLLWDVLDVMRQAGVWPLAPP
                     LGSAEMWPAPLTADGVRFVTSDPAQSQDSITQTIAMHRTLAEFDDRGRLNRENLIAMP
                     QKEHWHPRMMWYGPSGIGTTRGLDGFVDGHQLPFRIAFHRPQGTVDQVQAEKARHGAG
                     HYIRIGDGPYSVTGGWPSVYALHAGGGFCGLPPSGKPVFMRVMDFYLHHEGLIRENWV
                     PLDMLDLMAQMGCDALGRLREVLRR"
     gene            5579..6727
                     /locus_tag="PK812_02620"
     CDS             5579..6727
                     /locus_tag="PK812_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007199015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRJ68475.1"
                     /translation="MSIVGNEFPAIKTVAANAAKCQTGSLTVKSNLTANHQRINLPGM
                     QGNPAEYTSAIVATSSIVALINDDVIRPLDALVAAHGQDIPKNQLVTINGKVMAVAFM
                     ANSQHLVYRKDVLAQVGAEPPKTYEDMIRVGKLIRERGIMKNPIGGAYKAGWSLAQEF
                     VNMYIGHGGEFFKAGSAEVAINNAQGIAALNTMKALTELMHPDFLTHDNNETNAEWKA
                     GNVAMMNMWGSRTGVLMAKEGNDPKVAENTTVGAPMTVGGGKTPATTLWWDGWTVAKN
                     ISDDDARATFLAMKNGISPSILNDETMGQAVWMIAGYKPAAVNQGVMQTMAMGAKPYP
                     MLPYMGLLHTALQDGIAPFLQGRKDAATALSDIEKAYAAAAKEKGFLK"
     gene            6846..7868
                     /locus_tag="PK812_02625"
     CDS             6846..7868
                     /locus_tag="PK812_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRJ68476.1"
                     /translation="MRHATFLRFFLPTATAMLLFIALPIISVVIQSVYAPHDQVVIEV
                     QNCTPFGCTKSTAIDQDATKKLREEKPLGRFVGGAIYTDRAHLAFSELGEAWQLASGP
                     GDFLSRVMNLPFYRALVFTLTYMAFVTPLSILLGFIVAMTVNAIPRLMRGPVIFFSLL
                     PMIVTPLVGALILFWMVDSRGILGAAVQWLANNPNLSLKASTTLTWIMLIVYGTWSTS
                     PFAFVVYYAGLQTVPKDTLESAMLDGASRWHRVRYIILPHLMPLTVFMFLIKIMDNFR
                     VFEPIVSFNAGAHAQSLSYFIYSDLGGETRLLSSAAATSVLTIGGVLVLLSPALVRTW
                     RQFGKA"
     gene            7879..8760
                     /locus_tag="PK812_02630"
     CDS             7879..8760
                     /locus_tag="PK812_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009466711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRJ68477.1"
                     /translation="MSGSGLKRPLTLNLILGTFLVLWLVIAIFPFAWTLWGSFKVEND
                     FFSKADWSHALTGPLTQATHGSPFTGAGYEGAWIKEEFWRAALNSLIVVVCVVTISLT
                     FGTLGGYALSRSTHRYAFWLLIVALVFRAMPPITLVSGYLLPFYQWNLWGHLPTAIIV
                     LVAINQPFTLWMLTAFFKNIPKDLDESARVDGCTPFQAFRHVIIPVMWPGVITTGLFS
                     FLLAYNDFVVTAMLLSKQNQTMIPKIASFLGTTQTEGNVMFAVSSVVSVTAPLFLLVM
                     FFQRQIVSGLTAGAVKG"
     gene            8764..9822
                     /gene="ugpC"
                     /locus_tag="PK812_02635"
     CDS             8764..9822
                     /gene="ugpC"
                     /locus_tag="PK812_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HRJ68478.1"
                     /translation="MADIRLTNLSKRWGTFNAVRTMDLHIGDGEFLVLLGPSGCGKTT
                     TMRMIAGLEDVTEGEIRIGDRVVNDLDPKDRDIAMVFQSYGLYPTMTVYENIRFPLKV
                     RKIPEADHHERIMRAARTVELTELLDRKPAALSGGQRQRVALARAIVRKPKVFLMDEP
                     LSNLDAKLRVSTRAQIKNLHHELKTTTIYVTHDQIEAMTLADRVVVMNKGEVQQVGTP
                     LEIYDNPSNTFVASFIGNPAMNLISGDMRAGSFSAPGVSISGLAAPDGPVTLGFRAED
                     AQVGAAGDITAPVYSMELLGDSSMVTLQLGGGAFAAIKAAKDFKAAIGSTLSVRVPAG
                     ICHLFDARTGQRIARSAP"
     gene            9819..10811
                     /locus_tag="PK812_02640"
     CDS             9819..10811
                     /locus_tag="PK812_02640"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HRJ68479.1"
                     /translation="MSIDAGLALKRRYADHLTAFATPGADRWKAATRHFFAPDAVINT
                     VHPFNASTGPDAHWASVHEPLLAAFDGLYRRVDILMGGHFENADWVSATGYLVGHFQA
                     PFLGIRPTHRMAHLRFGEFHRMDKGQAVESYIFLDLPELMMAAGQWPLPMPPGHTGIL
                     QGPATQDGLQLHVNDPAQGSRSFTQVTEMLSKLNTPDEAWRPYWHPNMVWYGPAAFGT
                     YLGVDRFSGFQRPFEQCFEGWAGGSAGSGRTRHFTRFGDGLYTCSGGWPSLSGTSVRP
                     FLGQQPTGRMTFFRVCDWWRREGDLLMENWVFVDIPYAMLQFGVDVFAALQVRS"
     gene            10808..11317
                     /locus_tag="PK812_02645"
     CDS             10808..11317
                     /locus_tag="PK812_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007199011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HRJ68480.1"
                     /translation="MSDALEHGARSAATLAVIQRMEAALGANSNDMAQHFHEDFVWRG
                     NAGCGVKKGLAEFRRNWQLPLRAAFTDRVYRTEKFLADGDWASCFGHIEATHSGPFMG
                     IAPTGRRVIIPYMDFWRVADGRIADNPVFVDFAAVLAQLGRDVFAGEGWEAFDRGEKI
                     PPAPPGITP"
     gene            11329..12639
                     /gene="hisD"
                     /locus_tag="PK812_02650"
     CDS             11329..12639
                     /gene="hisD"
                     /locus_tag="PK812_02650"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018517387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HRJ68481.1"
                     /translation="MAIKTLKSGKPEQERAEDDAKVRATVESILKDIETRGDAAVREL
                     ALKFDRYAPPAFRLTSSEIEAAMAKVAPRDMEDIRFAQAQIRRFAEAQRASMTDIEVE
                     TLPGVILGHRNIPVQSVGCYVPGGKFPMVASAHMSVLTASVAGVPRIIASAPPVNGEP
                     HPAIVAAMHMGGAHEIYVLGGIQAVGAMALGTETISPVDMLVGPGNAFVAEAKRQLYG
                     RVGIDLFAGPTETMVIADETVDAELCATDLLGQAEHGYNSPACLITNSRRLAEGTLRE
                     VERLLKILPTAETAAVSWRDYGDVILCDTHEEMLQVANDMAYEHVQVMTDRDDWYLKN
                     MHSYGALFLGARTNVANGDKVIGTNHTLPTRKAGRYTGGLWVGKFLKTHSYQKVLTDE
                     AATLVGEHGSRLCMLEGFIGHAEQCNIRVRRYGRKNVPYGMAAQ"
     gene            12639..13421
                     /locus_tag="PK812_02655"
     CDS             12639..13421
                     /locus_tag="PK812_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRJ68482.1"
                     /translation="MATPARPVLPRTPSFRLDGKRALVTGASSGIGLGAAAALAEAGA
                     RVLLVARRENELDLVTKAIVAAGGMAEFAVLDITDIAATADFIARSGPFDVLVNSAGL
                     ARHAPALSTTPEDYDAAMNLNLRAAYFLTREVARGLIAAGKPGSLINVSSQMGHIGGP
                     DRAVYCANKHALEGMTKAMALEWASSGIRVNTLCPTFIRTPLAEQTLAIPERKAWILS
                     KIKLGRVGEIEDLMGPVVFLASDASALMTGTHLVIDGGWTAE"
     gene            13418..14449
                     /locus_tag="PK812_02660"
     CDS             13418..14449
                     /locus_tag="PK812_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009466716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRJ68483.1"
                     /translation="MSSQRPLEKVTSADVARLAGVSQSAVSRVFTPGASASPKTAAKV
                     RAAAERLGYRPNVLARAMITGRSRIIGLVVAYLENQFYPVALERLSSALQERGYHILV
                     FLAGNAAENVENVLGELLDYQVDGIITASVGMTNDLTSRCQTAGIPVVMFNRGQNDPR
                     LSEVTSDNRAGGRRVAEFLIAGGHKRIAHIMGWQGSSTGRDRAEGFRDALSAAGLAPF
                     AMIDGEYSRERAAAVARGLCGGRERPDAIFVGNDHMAFAVIDELVHGLRLRVPEDVSI
                     VGYDDVPMAGWPSYNLTTIQQPVDAMVDATIGTLIGQIEGNTEPCQFRIDGPLIVRQS
                     ARIPEGYRP"
     gene            14446..15429
                     /locus_tag="PK812_02665"
     CDS             14446..15429
                     /locus_tag="PK812_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009466717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HRJ68484.1"
                     /translation="MSTFSNRWPDFRDYIIDITREIWEGRGIGTLNHYYAPDIPVRSP
                     MGLSHGNQAVIAATMATCHEFPDRQLLAEDVIYSEHDEHGFLSSHRIISTGTHAADGA
                     FGSATGKRFTVRVIADCAARNDTIYDEWLVRDYGGIVRQLGMKPDDYARALIQREGGA
                     GKASRPLTPESDVPNVYKGRGNTNEWGVRYADILTRIMAVDFAHVHAAYDRAVIGEYA
                     GASQALGRDAVTAFWVGLRSAFPSATFTIHHQIGMDGGMLSPRAAIRWSLDGTHDGWG
                     AFGRPTGARVHVMGMSHAEFGPFGPDGIGLRREFALYDEIAIWKQIALHAG"
     gene            15518..16831
                     /locus_tag="PK812_02670"
     CDS             15518..16831
                     /locus_tag="PK812_02670"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HRJ68485.1"
                     /translation="MINRRTFGLGAATLGFGGLLPGSAWSQAAALANLKPGKPHAGTE
                     IKILCVVATQFRAHEARAAAFTEATGITVKYTYVPFANMREALTAEMVGGTGGYDMVV
                     VMDQWVPSLANLMEPMDERITTKKIDLKRYPEAFLKLGQVGGKQLGLPTRAHVQLLFY
                     RKDLFAKHNLQAPKTWDDVVKISKVLQEKENIAGIAMPYGKNQGQNLMVWYNMLWGRG
                     ADVFDAQGMPAFASEAGLKATQDYIDMLRTHKVTPAAAVSFNEQDAVNSMRGGNAAMV
                     PVWWWVRGGLLDPKVSKLTEEQVGFAPLPSYPGGKSSTYINCWIYALTKGSKNKDAAM
                     EFVTFVSDPSVERDILLDPKESDVVAVQYPNLRDADVNKRFGGMHTFAAEALQNTKVV
                     PYSADWPQMMEALETAMSEAASGAKSVPDAFKAADAAIRRIVRRG"
     gene            16874..17764
                     /locus_tag="PK812_02675"
     CDS             16874..17764
                     /locus_tag="PK812_02675"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRJ68486.1"
                     /translation="MQAPDRFLRYGLIAPALVLVIGTTLWPVVSAVTTSLRDWRLKRS
                     SLPQKWVGFDNYLVAFEEPEFWNAVKVTTTFVILDVLLTVCLALAVALLLRRAGVLQS
                     LVRAVIILPFAVSPALIGVSFRFMLNGEHGVLARGFAATFPWLSDVVWLATPGYAMAA
                     LIASDVWHWFPYLTLMFLGGLAAIPKETEEAAELDGANGLQMIFLVILPQIRSIIAVG
                     AILKTIFALKMFDQVATLTGGGPGITTQTLAHYTYAVGFVHYDMGLASALAVILTAIL
                     VTIGIFYIRLVLPQREHSAP"
     gene            17761..18579
                     /locus_tag="PK812_02680"
     CDS             17761..18579
                     /locus_tag="PK812_02680"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRJ68487.1"
                     /translation="MSDAPRPPVLVSAALILLFGIIVFPIAWMVLTAFKQPRDVYSLT
                     FVFAPTLENFITVFQPPWNIGRRIVNSLLIAGGTLAIAIPAAILAAYSFSRFQFMFKR
                     SLFFLVLATQFVPAVVVILPFYLMFRDLGLLDTRIGLIIVNLAIVTPFAIWMLKGFFD
                     AVPVECDEAALIDGASRLDIIRMVIVPMAMPGIIVASVFCFILTWNEFIFALILARDN
                     AVTLQVGLVNFRTERGDLWELMAAAGVFITVPAFIAALSIQKHFEAGLTGGAVK"
     gene            18576..19280
                     /locus_tag="PK812_02685"
     CDS             18576..19280
                     /locus_tag="PK812_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HRJ68488.1"
                     /translation="MNRELDPKVFAALRAVDTPTICNAIEAIEGRRRADGFTQSPVVC
                     ADVNLPAMVGFAKTAVIRSEAPASRPAAEMRDIRLNYYRYVAAGGPHRLVVIEDRGGR
                     KGVGAFWGEVNVALHKGLGLQGVLTDGSIRDLGAIDAGFQLLAGSVGPSHAFVHVAEF
                     DTPVTVFGLLVKPGDLIHADRHGAVVIEPGIEKDLPRAIDLVTRKEQPLLKAARAPDF
                     NIDKLLSAWGEAEDVH"
     gene            19317..20279
                     /locus_tag="PK812_02690"
     CDS             19317..20279
                     /locus_tag="PK812_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008034762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRJ68489.1"
                     /translation="MVDMMSRIVRYGELKPCKTAFIDAHTPGSNQKENFTIIGAGVSE
                     SADQHVHIRETPGFNIGAAGQPPKCRNSLHSHRTAEVFFVLKGRWRFFWGRWGTAGEV
                     ILEEGDIFNIPTGIFRGFENIGTDYGMIMAILGGDDAGGGVIWAPQVIEDAKAHGLVL
                     GANGRLYDTKKGQALPKGVKPMPLLTDKELKAFPEVPVEGVVRNYVARYWDLMALAAG
                     KPCKVIGAEAVLKDRPGFEVELLTRGSISEKPYSMKRHEILMPVRGHWRLAWEGGETI
                     LNPGDTCAVPPGLKHSLVPSMTGECSLYRVTNTKDKAGPTRVIN"
     gene            20288..21415
                     /locus_tag="PK812_02695"
     CDS             20288..21415
                     /locus_tag="PK812_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007199006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HRJ68490.1"
                     /translation="MTIQTTHVGSLPRPQKVADLLFGREKGLPFDEKPYDAVMKEATV
                     AIVAAQKAAMVDIPSDGETSKISYATYIKDRLTGFDGDSPRRPPQDLDDYPTYRDRLA
                     RAGGTPSYRRPKCVGPIKLKTLDPLKKDIAAMKAAMKKAGYRKGFMNAASPGVIALFQ
                     PSDYHKDMDAYLGDLSDAMRHEYEAIVEAGLILQIDAPDLGLGRHMMYRELDEKKFLK
                     RFAVHVEAINNALRNVPRDRVRMHICWGNYEGPHTRDIALKAVLPGLLKSKPGALLFE
                     GANPRHAHEWTVWRERKLPDDLVLIPGCIDSTTNFVEHPELVAQRLANYIGAVGVDRV
                     IAGTDCGFSTFAGFGVVEPEITWAKLRSLKEGAEIAATRAR"
     gene            21425..22111
                     /locus_tag="PK812_02700"
     CDS             21425..22111
                     /locus_tag="PK812_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009466720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRJ68491.1"
                     /translation="MLPLVLIPGMMCDARMWGAPGELGRRMVVHAACTEYDTMPALAS
                     AILADAPRRFALAGLSMGGIVAMEMLRQAPERIERLALLDTNPFAEQPEARARRQGQI
                     DRALGGALASVMRDEMKPNYLAPGPGNIPVLDLCLAMALALGPEVFARQSRALRDRSD
                     QSETLRSFRGPALVLLGRHDSLCPLQRHEAMQAMMPQARLAVIEGAGHLPTLEQPAAT
                     WAELTRWLEA"
     gene            22220..23905
                     /locus_tag="PK812_02705"
     CDS             22220..23905
                     /locus_tag="PK812_02705"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015930966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HRJ68492.1"
                     /translation="MATDIEIAREARLKPIAAIAAKIGVPESAVSPYGRHIGKIDLAH
                     VETLGDRPTGKLILVTAISPTPAGEGKTTTTVGLGDGLGRIGRKAMICLREPSLGPCF
                     GVKGGAAGGGHAQVVPMEQINLHFTGDFHAIGSAHNLLSAMIENHIYWGNALQFDTRR
                     VAWRRAIDMNDRSLRSIVSSLGGATNGFPREDGFDITVASEVMAVFCLATGLRDLEER
                     LGRMVVGHTRDRTAILARDVKAAGAMTVLLKDALSPNLVQTLEGNPAFVHGGPFGNIA
                     HGCNSVIATGAALRLADYVVTEAGFGADLGAEKFFNIKCRSAGFTPAAAVIVATVRAL
                     KMHGGVQKDALGGEDLKALAKGLSNLRRHVQNVRKFGVPAVVAINHFTSDTPAEHRLI
                     QEYCRTELGVEAVVCSHWADGSRGTEELAHKVVALAETPAPLDGRGFRPLYPLDMPLW
                     DKMRTIATEIYGASDIIADKKVRDQFRTLEAEGYGNLPICVAKTQYSFSTDPALRGAP
                     SGHIVPIREITLSAGAGFLVVVCGEIMRMPGLPRVPSAEKIHIDQQGRIEGLF"
     gene            24051..>25918
                     /locus_tag="PK812_02710"
     CDS             24051..>25918
                     /locus_tag="PK812_02710"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HRJ68493.1"
                     /translation="MSLSTARVASGLAKDYRPEVDGLRAVAVLAVIVYHAELPWLRGG
                     FAGVDVFFVVSGYLITRLILAERDAGHFSLLRFFERRARRILPALYLVIAACLPFAWM
                     LMFPEELRRFGDSVLSVLLLVANFYFVNTQDYFAPGAELSPLLHAWSLSAEEQFYLVF
                     PILALATLSISRRAFVGCILCLATASLGLAQFGGLLHENAGGLRWLAPPAWGYYLLPT
                     RAWELLLGAALAAGRFEQTALNARLPGLLRQTLGIAGIALIVASCLFYRRSTPFPTAY
                     ALVPTIGTALVILFARQGTLAHAALAWRPLVGIGLMSYSLYLWHQPVLAFSRLYLPRG
                     MSEVWLVGAVALSFLLAWLGWRFVEQPCRDRAAVSTRAFALGVILSGLALGAAGYACR
                     VVGADPMLLSRAGAISARVDHAVGLSPACVYFGSWHHKPECATGDEPEAIVWGDSFAA
                     HTVNAIVAANPGLPLLQATMPVCGPIPGIAPVVNEYDESWGRRCIQFNQAVLEHVLAS
                     PSIREVILGIHFANYIWGEQKLFDGTRVRSASPERLRGELRAAIGRLRAAGKRVTLIS
                     SPARSLFDVGRCLALATWRSLPGEICDFSYSDHPQNAFIDAFLRSLEGEGVHVIALR"
BASE COUNT         4420 a         8252 c         8412 g         4834 t
ORIGIN      
        1 tccatccggc gcgaccttca gggcggcgcc gtcgagggca ctcgacagat cgccggtcct
       61 tgagcgcagg acgacgacgc cgcccgcagc gacaaacagc ctgcggtcgg cgagcgcgcc
      121 aaggatatcc gaagctctgg gatcgccact ggcggcgatg atgtcgatgg ccttggccgt
      181 ctccgcgaag ctgtcgccgg ccagcagcgg gccggcttca gccaagggcc ggctggcggc
      241 tgacgccacc accgcgaagg cgagcatcaa gccatacagc gccgacacaa tcgtggtgaa
      301 gcggatggtc atgtcggctt tcttcccagg cgataggcag tcgggaactc tgccccgcat
      361 cgggtgtggc ccggatgcag ggcgaagcgt tcagcagcgt gcggtcagcc gcacttgttg
      421 gtcttcatgt tgaagttgcc gcacttcttg ccgacccagt cggcctcgag gtccttcgaa
      481 ccttcaaggt gatcggtcca ggcatcgccc ggaaccaggt ccttcgtctg ccaaaccacg
      541 tcgaactggc cgtcgccgcg cacttcgccg atcagcacgg gcttggtgat gtggtggttg
      601 gccagcatct tggacatgcc gccggtcagg ttcggcacct cgatgccggg cagcgcatcg
      661 atgaccttgt cgatgtcggt ggtcttcact ttttccaccg ccttcaccca catggcgaac
      721 ccgatcacat gggcttccat cggatcgttg gtcacagcct tgtcgctctt cttgaaggcc
      781 ttccactgct tgatgaaggc ttcgttcgcg gggttcttca cgctctggaa atagttccag
      841 gccgcgaggt gaccgacgag cggcttggtg tcgatgccgg ccagttcctc ttcgccgacc
      901 gagaaggcga cgaccgggat gtcggtcgcc ttgatgccct ggttgcccag ttccttgtag
      961 aacggcacgt tggcgtcgcc attgatggtc gacaccacgg cggtcttctt gccggcggaa
     1021 ccgaatttct tgatgtcgga tacgatcgtc tgccagtcgg aatgaccgaa gggcgtatag
     1081 ttgatcatga tgtcttcctt ggccacgccc ttcgaaagga gataggcctc aaggatcttg
     1141 tttgtcgtgc gcgggtagac atagtccgtc ccggcaagca cccagcgttt ggcctcgccg
     1201 ccatccttcg acatcaggta gtcgacggcc gggatggcct gctggttcgg tgcggcgcca
     1261 gtgtagaaca cgttgcgctc gctttcctcg ccctcatatt gcacggggta gaacaggatg
     1321 ttgttcaatt ccttgaacac cggcaggacc gacttgcgcg acacggatgt ccagcatccg
     1381 aaagtggcgg caaccttgtg ttgcgtgatc agttcccggg ccttttcggc aaagagcggc
     1441 cagttcgagg cggggtcgac gaccacggct tccagcttct tgccaagcag gccgcccttg
     1501 gcgttctgtt cggcgatcag catcagcatg acgtccttca gggtcgtctc ggaaatcgcc
     1561 atcgtgccgg atagcgagtg aaggattcca accttgatgg tgtcaccgga ctgggcgaag
     1621 acaggaaccg aagggagagc ggaggctgcc gttgcggcaa tggtggacgc gagcaggctg
     1681 cggcggctga gggtcatttc ggaaactcct tggaggtgga cgttcccatg ccaagcatcc
     1741 gctgtgccaa accaactatc tatttgaatt ataatagttt tttctggagc gatgccggca
     1801 tatgccgacg aattaggcag gcgaaatgcc aacaacgcgg gcagctgcat acaattcggc
     1861 ttgtcgcgcc gaagttcgcg cagggcgctt ggttttccat caagatggcc acggtttggg
     1921 cactcgcgcc ggcagagcgg aaaccggttc accgtaaagc cggccgatca aaagaacgcc
     1981 agcggctctt gcgttcctgg atcaggtcgt gcccactagg gatgcgtggc gccggtcccg
     2041 ccgcaggcag gttaacggac atgaagcatt tgatcattgc ggcgatggca gtcgtcgcga
     2101 cacttcaggt ggcgatgtca caggttccga cggggctccg gctggtgtcg gacacgccgc
     2161 gcggccatct tggcgagacg atcagcggcg atgcgtttgt cgagacccag cgccgtctgc
     2221 tcatgctggt gacgttcgac aaggccgccg ccgggcagcc catggatgtc tgggttcgtg
     2281 ccatgaagac caccgccagc cggagcgccc tgctgatttc cggcagtgtc aagatcgatg
     2341 ccgaaggccg tattccattg acggtcagcc tgccgcgcca gtggcccgtg ggcgaatacg
     2401 tggtgcaggt cgccaggccg ggcgagaagc cggtcgagtt gccgttccgc gttcggccgg
     2461 aaagcccccg cagcacgccg ctgaaggtca gcgcaattga gttggaccgc attgtcggag
     2521 cgggaaagag cgagcgggtc cagcgtttcc tgccttccga caggcactgg aatttcggcg
     2581 ccacagtcga gggcgcgcgc accgaaggcg tcgatgtgcg ctgggtgctg acggccgttc
     2641 agaccacatc cggctcggcc gaagtcggca agatcgatgt gccgcgccgc cccatcgaga
     2701 acacggagct gcagttcgac atggagttgc cgcgcgactg gccggttggc cggtacaggg
     2761 tggagctgtt tctgaacggc aagagtgagg cgatgaagga attcgatgtc aggccgtgat
     2821 gcggcgggtg tcgccgacaa ccttgtcgtg ccggactgct cgaacaacgc cgggcctctg
     2881 ggggcagggc ttgaggcggc ttgcgggttg ctcacggtag aagggctggt cggattctcg
     2941 tgaacccggc gcgtcccgtg gagggcctcg gattgggagg gttttcgatg cagtacaccg
     3001 ggatcaggcg ctggatcata gcgctgtcac tggccgggtt ggccagcttc gcgccggacg
     3061 ccctggcgca gggcgcttcc gaaacgacac gcacgcggga agggacacgc gtcaccggca
     3121 ccgggggcaa cttcggcggc ggctacgacg tcgatcgtcc cggccggcgc gtcaccggaa
     3181 caggacgcaa tttcgggagc ggctatgagg tcgaccggtc cgggcgtcgc atgaccggta
     3241 ccggccggaa cttcggcagc ggctatgaga cgtcgcgcag tcgacagcgc attaccggta
     3301 ccggtcagaa tttcggcaag ggctggatgc gttccaacaa cggcaaggag tggctcggta
     3361 ccggcgccaa cttcggcaag cgttgtccgg tgacggcgac gccgccgacc gtttcctgtt
     3421 gacgacgcat gcccgtccgc ggtgttcgtc ccggcgcttc gcatgaaagt cgcacgcaac
     3481 gggcgtgcag gcatggagaa agcagatgac acggtatgcc ttggctgccc ttttcgttct
     3541 cggccacctt gctcctggat tcgcccaggc gccggcccct tcagatggga cggccacggc
     3601 ctcctacatc cgcgccaagc ctgagcagat gctcgaggtc tatccgcgcg aggtggtgac
     3661 gcgggtgttt tcgaacgaat tcacggaggc gcgtcgctcc aaggtgatgt cgacgatcca
     3721 gggcgcgccg ggcgtgaact gccccgagcg gccgaacgtg gtggcctatg ccgtgatccc
     3781 cttcaagctt ggcaaggacc agaccacctg gatcgagcgc tacgccgttg gctgtgacaa
     3841 ggcgatcggg cggaacttcc tggtcattct ggagcctgcc ggagtcaaac tcctggaact
     3901 cgtgcgcggc gcgacgcgga ccgacccgtt cctgcagcgc gatctcggcg ggcctgtcgg
     3961 cggcatcgcg cagtcacggc gcccgcaggg ctgccagagc cacatcatcg ccgacaccgc
     4021 catcacgcag cagccgcccc agggcggcgg cccgtgggtg gagaaatggg acattgccgc
     4081 ctgcgatcat cgctcgtcca tcgagatcac attcacccct tcggcgcagg ggggaaccac
     4141 ctggaacatc aagccgtgac cgaatgccgg gatgggtggt cgacgtttca gtccctggca
     4201 tccgcttcac gcgcgggggg ctagcggcgc agcacctcgc gcaagcgccc cagggcgtcg
     4261 catcccatct gggccatgag atcgagcatg tcgagcggca cccagttctc gcggatcagc
     4321 ccctcgtggt gcaggtagaa gtccatcacg cgcatgaaca ccggcttgcc cgacggcggg
     4381 agcccgcaga aaccgccgcc ggcatggagt gcgtaaaccg atggccagcc gcccgtgacg
     4441 gaataaggcc cgtcgccgat gcggatatag tggccggcgc cgtggcgggc cttctccgcc
     4501 tgtacctggt cgaccgttcc ctgcggccgg tgaaaggcga tccggaacgg cagctggtgc
     4561 ccgtcgacaa agccatccag accgcgtgtc gtccctatgc ctgagggtcc gtaccacatc
     4621 atcctgggat gccagtgctc cttctggggc atggcaatca ggttctcgcg gttgagccgg
     4681 ccccgatcat cgaattcggc cagcgttcgg tgcatcgcga tggtctgcgt gatgctgtcc
     4741 tgggactgcg cggggtctga cgtcacgaag cgtacgccat ccgccgtcag cggcgccggc
     4801 cacatttccg cgctgccgag cggcggtgcg agcggccaga ccccagcctg ccgcatcacg
     4861 tcgagcacat cccacagcag gttggcctgc acgacggcgc cttgcgcatc gacctcatag
     4921 acttcgccat agcgcaggta gatcggtctg ttggtcgccg ggatgccgag ccagtcgccg
     4981 cgcatcgttc cacaatagtg cccgaccgct gccagataga tgcggcgctg gtaggtgccg
     5041 ccgacgaaga tgacgtcccg ccgctcaagg tcgggcaggg cgcgcttcag cggagcgaaa
     5101 gcgcgggtga gcgcctcggc attgccggcg caagagttga tcgggtgcgc gacgcgccac
     5161 aggcaatccg gtcttgcgag cgccgaaagc gccccatcaa ggctctcgtc ccgcgcttcg
     5221 gccagacgcg cgagtgcgcc atgcaccgtc cggcgcaagg cgggcgcggg ggatgcgtgc
     5281 gggtcaaaat cgggaaccat gggacttccg gtcagggttg atgcgagcac cgcgatccga
     5341 cggcgcaggc gcagtattcc ggaagtcgca gacgccatca agataagact tgcatacgat
     5401 tgcaagttgt gatcggaatg ggtggaagct gccgcatgcc gagaaccgga gacggttggc
     5461 cgcgattggg agggaagagg gaatgatcag gacactcagg acagcattgt cacttgcggc
     5521 gctactcgcg gccggctctg ttcaggctca agtgaattgc ggcgcgacga ccgggcgcgt
     5581 gagcattgtc ggcaatgagt tcccggcgat caagacggtg gccgcgaacg ccgccaagtg
     5641 ccagaccggt tcgctgacgg tgaagtcgaa cctcacggcc aatcaccagc gcatcaacct
     5701 gcccggcatg cagggcaacc cggcggaata cacctccgcc atcgtggcga cgtcgtcgat
     5761 cgttgcgctg atcaatgacg atgtgatccg cccgctcgat gcgctggtgg cggcccacgg
     5821 tcaggacatt cccaagaacc agctcgtcac gatcaacggc aaggtgatgg ccgtggcgtt
     5881 catggccaat tcgcagcatc tggtctatcg caaggatgtg ctggcgcagg tcggcgccga
     5941 gccgccaaag acctatgagg acatgatccg cgtcggcaag ctgatccgcg agcgcggcat
     6001 catgaagaac ccgatcggcg gcgcctataa ggccggctgg tccctcgccc aggaattcgt
     6061 caacatgtac atcggccacg gcggcgagtt cttcaaggcc ggctcggcgg aagtggcgat
     6121 caacaacgcg cagggcatcg cggcgctgaa cacgatgaag gcgctgaccg aactgatgca
     6181 tcccgatttc ctcacgcatg acaacaacga gaccaatgcc gaatggaaag ccggcaacgt
     6241 cgcgatgatg aacatgtggg gttcgcggac cggcgtgctg atggcgaagg aaggcaatga
     6301 tccgaaggtc gcggagaaca cgaccgtcgg cgccccgatg acggtcggcg gcggcaagac
     6361 tccggccacc acgctctggt gggacggctg gaccgtggcg aagaatatct ccgatgacga
     6421 cgcccgtgcc acgttcctcg ccatgaagaa tggcatctcg ccctcgatcc tcaatgacga
     6481 gaccatgggg caggcggtat ggatgatcgc aggctacaag cccgccgccg tcaatcaggg
     6541 cgtgatgcag accatggcca tgggcgccaa gccgtatccg atgctcccct acatgggtct
     6601 gctgcatacc gccctccagg atggcatcgc gcccttcctc cagggccgca aggatgccgc
     6661 gaccgctctc tccgatatcg agaaggccta tgcggcagcc gcgaaggaaa agggcttcct
     6721 gaagtaagcg gttcctctcc cgcaagtacg gcgacggcct ttctgccgtc gccggtcgct
     6781 tccgaccgat cactcggccg caggtgcgcg gctcacgcgc agcatggatg cgcgaaggag
     6841 gaggcatgag gcacgcgacc ttcctgcgct ttttcctccc gacggcgacg gcgatgctgc
     6901 tgttcatcgc cctgccgatc atctcggtgg tgatccagtc cgtctatgcg ccgcacgatc
     6961 aggtcgtgat cgaagtgcag aactgcacgc cattcggctg caccaagtcc acggcgatcg
     7021 atcaggacgc gacgaaaaag ctgcgtgaag aaaagccgct cgggcgtttc gtcggcgggg
     7081 cgatctacac cgaccgcgcc catctcgcct tttctgaact tggcgaagcc tggcagctgg
     7141 cgtccggacc cggcgatttc ctgtcgcgcg ttatgaacct gcccttctac cgggcgctgg
     7201 tcttcacgct cacctacatg gcattcgtca cgccactctc tatcctgctc gggttcatcg
     7261 tggcgatgac ggtcaacgcc atcccccgtc tgatgcgcgg gccggtgatc ttcttctcgc
     7321 tgctgccgat gatcgtcacg cccctggtgg gagcgctgat cctgttctgg atggtggatt
     7381 cccgcggcat tctcggggct gccgtgcaat ggctggcgaa caacccgaac ctgtcgctga
     7441 aggcgtccac gacgctgacc tggatcatgc tgatcgtcta cggcacctgg tccacctcac
     7501 ccttcgcctt cgtggtctac tatgccgggc ttcagaccgt gccgaaggac acgctggaga
     7561 gcgccatgct tgacggcgcc tcgcgctggc accgcgtccg ctacatcatc ctgccccacc
     7621 tgatgccgct gaccgtgttc atgttcctca tcaagatcat ggacaacttc cgtgtcttcg
     7681 agccgatcgt cagcttcaat gcgggcgcgc acgcgcagtc actgtcctat ttcatctatt
     7741 ccgatcttgg aggcgagacg cggctgctgt cgtcagccgc cgccacctcg gtcctcacca
     7801 tcggcggcgt cctggttctt ctctcgccgg cgctggtgcg cacctggcgg caattcggca
     7861 aggcgtaagg agtccccgat gtccggcagc ggcctgaagc gccccctcac tctcaacctg
     7921 atcctcggca cgtttctggt gctgtggctg gtgattgcca ttttcccgtt cgcctggacg
     7981 ctctggggca gtttcaaggt ggagaacgac tttttctcca aggcggactg gagccatgcc
     8041 ctgaccggac cgctgacgca ggccacgcat ggcagcccgt tcaccggcgc gggctacgag
     8101 ggggcctgga tcaaggagga gttctggcgc gcggctctga attcgctgat cgtggttgta
     8161 tgcgtggtga cgatctcgct tactttcggc acgcttggtg gctacgcgct cagccgctcg
     8221 acccaccgct acgccttctg gctgctgatc gtggcgctgg tgtttcgcgc catgccgccg
     8281 atcacgctgg tttccggtta cctgctgccc ttctaccagt ggaatctctg gggacacctg
     8341 cctacggcga tcatcgtcct tgtggcgatc aaccagccgt tcacgttgtg gatgctgacg
     8401 gcgttcttca agaacattcc gaaggacctg gacgagagcg cccgcgtcga cgggtgcacg
     8461 ccgttccagg ccttccggca tgtgatcatc ccggtgatgt ggccgggcgt gattaccacg
     8521 gggctgttct ctttcctgct ggcctataac gacttcgtgg tcacggcgat gctgctgtcc
     8581 aagcagaacc agaccatgat tccgaagatc gcaagtttcc ttggaaccac gcagaccgag
     8641 ggcaacgtca tgttcgcggt ctcgtcggtc gtttccgtca ccgcgcccct gtttcttctg
     8701 gtgatgttct tccagcgtca gattgtctcc ggcctgacgg ccggcgcggt gaaaggctag
     8761 gccatggcgg atatccggct caccaatctg tccaagcgct ggggaacctt caacgcggtc
     8821 aggacgatgg atcttcacat cggcgacggc gagttccttg tgctgctcgg cccatctggg
     8881 tgcggcaaga ccacgaccat gcgcatgatc gccggactag aagatgtcac cgagggtgag
     8941 atccgcatcg gcgaccgggt ggtcaacgac ctcgatccga aagaccgcga catcgccatg
     9001 gtgttccagt cctatgggct gtacccgacg atgaccgtct acgagaacat ccgtttcccg
     9061 ctgaaggtgc gcaagatacc cgaggctgac catcacgagc gcatcatgcg cgcggccagg
     9121 acggtggaac tgacggaatt gctcgatcgc aagccggcgg cgctttccgg tggccagcgc
     9181 cagcgcgtcg cactggcacg cgccatcgtg cgaaagccca aggtcttcct gatggatgag
     9241 ccgctgtcca atcttgacgc caagctgcgc gtctcgacgc gggcgcagat caagaacctc
     9301 caccacgaac tgaagacgac cacgatctac gtcacgcacg accagatcga ggccatgaca
     9361 ctcgccgacc gtgtcgtggt gatgaacaag ggcgaggtgc agcaggttgg tacacccctt
     9421 gagatctatg acaacccgtc caacaccttc gtggcctcgt tcatcggcaa tcccgcgatg
     9481 aacctgattt ccggcgacat gcgtgcaggc tccttcagtg ctcccggcgt gtcgatcagc
     9541 ggcctggccg cgccggacgg gccggtgacg ctgggcttcc gtgccgagga cgctcaggtc
     9601 ggggcagcgg gagacataac ggcgccggtc tactctatgg aacttctggg cgattcctcc
     9661 atggtgacgc tgcaactggg gggcggcgcc ttcgcggcga tcaaggccgc gaaggacttc
     9721 aaggccgcga tcggcagcac gctgtccgtt cgggtcccgg ccggcatctg ccatctgttt
     9781 gatgcacgca cgggccaacg catcgctcgg agcgcgccat gagcatcgat gccggacttg
     9841 cgttgaaacg gcgctatgcg gaccatctca cggcattcgc cacgcccggc gccgaccgtt
     9901 ggaaagccgc gacgaggcac ttcttcgctc cggacgccgt catcaacacg gtgcatccct
     9961 tcaatgcctc gaccggaccg gatgcgcatt gggcctcggt ccacgaaccg cttcttgccg
    10021 ccttcgacgg tctgtatcgg cgggttgaca tcctgatggg gggacatttc gagaatgccg
    10081 actgggtgag cgccaccggg taccttgtcg gccatttcca ggcgccattc ctcggcattc
    10141 gccccacgca tcggatggcg cacctgcggt tcggcgagtt tcaccggatg gacaagggcc
    10201 aggctgtcga gagctacatc ttcctcgacc tgcccgaact catgatggcg gccgggcagt
    10261 ggcccttgcc gatgccgccg gggcacaccg ggatcctgca aggcccggcg acgcaggacg
    10321 gtctgcaact gcatgtcaac gaccctgcac agggctcccg ctcgttcacg caggtgaccg
    10381 agatgctctc gaagctgaac acgccagacg aggcctggcg tccctactgg caccccaaca
    10441 tggtctggta tggaccggcg gcattcggca cttatctcgg tgtcgaccgg ttctcggggt
    10501 tccagcgccc gttcgaacag tgtttcgaag gttgggccgg cgggtcggca gggtcaggga
    10561 gaaccaggca cttcacccgc ttcggggatg gtctctacac ctgttcgggc ggctggccgt
    10621 cgctctccgg cacctcggtc agaccgttcc ttggccagca gcctacgggc aggatgacct
    10681 tcttccgggt ctgcgactgg tggcgccgag aaggcgacct gctgatggag aactgggttt
    10741 ttgtcgacat tccctacgcc atgcttcagt tcggcgtgga tgtctttgct gcgctccagg
    10801 tgcggtcatg agcgacgctc tggagcatgg cgcgcgctcg gcggcgacac tggctgtgat
    10861 ccagcggatg gaggcggcgc ttggcgccaa ttccaacgac atggcgcagc atttccatga
    10921 ggacttcgtc tggcgtggca acgccggctg cggcgtgaag aagggtctgg ccgaattccg
    10981 ccgcaactgg cagctgccgc tgcgggcggc gttcacggat cgcgtctaca ggaccgagaa
    11041 gttcctggcc gatggagact gggcttcttg cttcggccat atcgaggcga cccattccgg
    11101 tcccttcatg ggcatcgccc cgaccggacg gcgcgtgatc atcccgtaca tggatttctg
    11161 gcgtgtcgcc gacgggcgca tcgccgacaa tccggttttc gttgattttg ccgccgtgct
    11221 tgcacagctt ggtcgcgatg tgttcgcggg cgaaggctgg gaagcctttg atcgcggcga
    11281 gaaaattccg ccggcaccgc cgggcataac tccatgagga cgtgactgat ggccatcaag
    11341 accctcaaat ccggcaagcc tgagcaggag cgggccgagg acgacgccaa ggttcgtgcg
    11401 accgtcgaat ccatcctcaa ggacatcgag acgcgcggcg atgcggcggt gcgcgaactg
    11461 gccttgaagt ttgatcgcta cgcgccaccg gctttccggc tgacatcctc cgagatcgag
    11521 gcggcgatgg cgaaggtcgc gccccgtgac atggaggaca tccgcttcgc ccaggcacag
    11581 atacggcgtt ttgccgaggc gcagcgcgcc agcatgacgg atatcgaggt cgagacgctt
    11641 cccggcgtga tccttggcca tcgcaacatt ccggtgcagt ccgtcggctg ttatgtgcct
    11701 ggcggcaaat ttccgatggt cgcctcggcg catatgtcgg tcttgaccgc ctcggttgcc
    11761 ggcgtcccgc gcatcatcgc atccgccccg ccggtcaatg gcgagccgca tcctgcgatc
    11821 gttgccgcca tgcacatggg cggagcgcac gagatctacg tgcttggggg cattcaggcg
    11881 gtcggcgcca tggcgctcgg caccgagacg atcagcccgg tcgacatgct tgttggccct
    11941 gggaacgcct ttgtggccga ggccaagcgc cagctctatg gccgcgtcgg catcgacctt
    12001 ttcgccggac cgaccgaaac catggtgatc gccgacgaga cggtcgatgc cgaactctgc
    12061 gcgacggacc tgctcggcca ggccgagcac ggatacaatt ctccagcgtg cctgatcaca
    12121 aattcgcgcc ggctcgccga gggcacattg cgcgaggtgg agcggttgct gaagatcctg
    12181 ccgaccgccg agaccgcagc cgtgagctgg cgcgactacg gtgacgtgat cctgtgcgac
    12241 acccacgagg agatgctgca ggtggccaac gacatggcct acgagcatgt ccaggtgatg
    12301 accgaccgag acgactggta cctgaagaac atgcattcct acggtgcgct gttcctgggc
    12361 gcgcgcacca acgtggccaa cggcgacaag gtgatcggta ccaatcatac cctgccgacc
    12421 aggaaggcag ggcgttacac gggggggctg tgggtgggca agttcctcaa gacccacagc
    12481 taccagaagg tgctgacgga cgaggcggcc acgcttgtcg gcgaacatgg ctcgcggctg
    12541 tgcatgctgg aaggcttcat tggccatgcc gagcagtgca acatccgtgt ccggcgttac
    12601 ggccggaaga acgtccccta cggcatggcg gcgcagtgat ggcaacgcct gctcgtcctg
    12661 tcctgccgcg cacgccgtcc ttccggctgg acggcaagcg tgcgctcgtg accggcgctt
    12721 cctcgggcat cggactgggg gctgccgcgg cgctcgcaga ggccggcgcg cgagtcctgc
    12781 tggtggcaag acgcgaaaac gaactggacc ttgtgacgaa agccatcgtc gccgccggcg
    12841 gcatggcgga gtttgcggtg ctcgacatca cggacatcgc cgccacggca gatttcatcg
    12901 cccgcagcgg cccgttcgac gtgctggtga actcggccgg gctggcccgt cacgccccgg
    12961 cgctctccac cacgccggag gattacgacg cggcgatgaa cctcaacctg cgcgccgctt
    13021 acttcctgac gcgcgaggtg gctcgcggcc tgatcgctgc gggaaagccg ggttctctga
    13081 tcaatgtctc gtcccagatg gggcatatcg gcgggccaga ccgcgccgtc tattgcgcca
    13141 acaagcatgc gctggagggg atgaccaagg ctatggcgct ggaatgggcg tcgagtggca
    13201 tccgcgtcaa caccttgtgc ccgaccttca ttcgcacgcc cctggccgag cagaccctcg
    13261 ccatccctga gcgcaaggcg tggatcctgt ccaagatcaa gctgggcagg gtcggcgaga
    13321 tcgaggacct gatggggccg gttgtgttcc ttgcatcgga tgcttccgcg ctcatgaccg
    13381 ggacccatct ggtgatcgat ggagggtgga cagcggaatg agcagccagc gccccctgga
    13441 aaaggtgacg agtgcggacg tggcgcgcct ggccggcgtg tcgcagtcgg cggtatcgcg
    13501 cgtgttcacg ccgggcgctt ccgcgtcgcc gaagacggct gccaaggttc gcgctgccgc
    13561 cgagcgactg ggctatcgcc cgaacgtcct ggcgcgcgcc atgatcaccg gccgcagccg
    13621 catcatcggc ctggtggtcg cctatctgga gaaccagttc tacccggtgg cgctggagcg
    13681 tctgtcaagc gccctgcagg agcggggcta tcacatcctg gtcttccttg cgggcaacgc
    13741 tgccgaaaat gttgagaatg tccttggaga acttctcgac tatcaggtcg acggcatcat
    13801 caccgcttcg gtcgggatga ccaacgacct cacctcccgg tgccagactg ccggcatccc
    13861 ggtggtgatg ttcaaccgcg ggcagaacga tccgcgcctc agcgaagtga cgtcggacaa
    13921 cagggccggc ggacggcgcg tcgccgagtt cctgatcgcc ggcggacaca agcgcatcgc
    13981 gcacatcatg ggctggcagg gctcatccac tggtcgcgac cgcgccgaag gctttcgcga
    14041 cgccttgtcg gcggccggcc tcgccccctt cgcgatgatc gacggcgaat attctcgtga
    14101 gcgcgcggcc gccgtagcgc gcggcctgtg cggcggcagg gagcggcccg acgcaatatt
    14161 cgtcggcaac gatcacatgg ccttcgcggt gatcgacgag cttgttcacg gcctcaggct
    14221 gcgcgtgccg gaggatgtct cgatcgtcgg ttacgacgac gtgccaatgg ccggctggcc
    14281 ttcctataac ctcacgacca tccaacagcc ggtggatgcc atggttgacg ccacgatcgg
    14341 caccttgatc ggccagatcg agggcaacac ggagccatgc cagttccgca tcgacggtcc
    14401 gttgatcgtc cgccagtcgg cccgcattcc cgagggatat cgcccgtgag cactttttcg
    14461 aaccggtggc cggatttccg cgactacatc atcgatatca cccgggaaat ctgggaaggt
    14521 cgcggcatag gcaccctgaa ccactactac gcgccggaca ttccggtgcg ttcgcccatg
    14581 ggcctcagcc atggcaatca ggccgtgatc gccgccacca tggcgacctg tcacgagttt
    14641 ccggatcgcc agctgctggc cgaggatgtc atctactccg agcacgacga acacggcttc
    14701 ctgtcgtcgc atcgcatcat ttcgaccggc acgcatgccg ccgacggcgc cttcggctcg
    14761 gcgacaggca agcggttcac ggtgcgtgtc atcgccgatt gcgcggccag gaacgacaca
    14821 atctacgatg aatggctggt gcgcgactat ggcggcattg tccgccagct cggcatgaag
    14881 ccggatgact acgcgcgcgc tctgatccag cgggaaggtg gcgcgggcaa ggcctcgcgc
    14941 ccgctgacgc cggagtccga cgtgcccaac gtctacaagg ggcgcggcaa taccaacgag
    15001 tggggcgtgc gctacgccga catcctcacg cggattatgg ctgtggactt cgcccatgtt
    15061 cacgctgctt atgatcgcgc cgtgatcggc gagtacgccg gcgccagcca ggcgctggga
    15121 cgcgacgcag tcaccgcctt ctgggttggg ctgcgctccg catttccgtc ggcgaccttc
    15181 accatccacc atcagatcgg catggatggc gggatgctct ctccgcgcgc cgcgatccgc
    15241 tggtcgctcg acggaacgca cgacgggtgg ggcgccttcg ggcggccgac cggcgcgcgc
    15301 gtgcatgtca tgggcatgag ccacgccgag tttgggccat tcggtccgga tgggatcggt
    15361 cttcgccgtg aattcgcgct ttatgacgaa atcgcgatct ggaagcagat cgcgcttcat
    15421 gccggctgag ttcgggcttg cgtcgaggcc cgttccgcgt aagtttgcaa tcgaatgcaa
    15481 aaaggtccgc aggacctatt ctgaggagga aaccgtgatg atcaatcgcc gtactttcgg
    15541 actgggggct gctacgctcg gctttggagg cctgcttcca ggatcggcct ggtcccaggc
    15601 tgccgcgctg gccaatctta agccgggaaa gccgcacgcg ggaaccgaga tcaagattct
    15661 ctgcgtggtc gcgacgcagt tccgcgccca cgaagcccgg gctgcggcct tcaccgaagc
    15721 gaccggcatc acggtaaaat acacctacgt ccccttcgcc aacatgcgcg aggcgctgac
    15781 ggccgagatg gtcggcggca ccggcggcta tgacatggtc gttgtgatgg accagtgggt
    15841 gccttcgctc gccaacctta tggagccgat ggacgagcgg atcacgacca agaagattga
    15901 tctcaagcgc taccccgagg ccttcctgaa gctgggtcag gtcggtggca agcagcttgg
    15961 cctgccgacg cgcgcgcacg tgcagttgct gttttaccgc aaggacctgt tcgccaagca
    16021 caacctgcag gcgcccaaga cctgggatga tgtcgtcaag atctcgaaag tactccagga
    16081 gaaggaaaac atcgctggca tcgccatgcc ctatggcaag aaccagggcc agaacctgat
    16141 ggtctggtac aacatgctgt ggggccgcgg cgctgacgtt ttcgacgctc agggaatgcc
    16201 ggccttcgcc agcgaggcgg ggctcaaggc gacgcaggac tacatcgaca tgctgcgcac
    16261 ccacaaggtg accccggcgg cggcggtgtc cttcaacgag caggatgcgg tgaactccat
    16321 gcgcggtggc aatgccgcaa tggttccggt ctggtggtgg gtgcgcggtg gcttgctgga
    16381 tccgaaagtg tccaagctca cggaagagca ggtgggtttt gccccgctgc cgagctatcc
    16441 cggcggcaag tcatcgacct acatcaactg ctggatctac gcgctcacga aaggctccaa
    16501 gaacaaggac gccgcgatgg agttcgtgac ctttgtttcg gatccctcgg ttgagcgtga
    16561 catcctgctc gacccgaagg aaagcgacgt tgtcgctgtg caatatccga acttgcgcga
    16621 cgcggacgtg aacaagcggt tcggcggcat gcataccttc gcggccgaag cgctccagaa
    16681 caccaaggtc gtgccctatt cggccgactg gccgcagatg atggaagcgc tggaaaccgc
    16741 catgtcggaa gccgcgtccg gcgcgaagtc ggtgcccgac gccttcaagg ctgcggacgc
    16801 agccatccgc cgcatcgtgc ggcgcggttg atgcaggagc tggcccggac ggatcgcgtc
    16861 cgggcctcct gacatgcagg cacctgaccg cttccttcgc tatggcctga ttgctccggc
    16921 gctggtgctg gtgatcggca cgacgctgtg gccggtcgtg tcggctgtca cgaccagcct
    16981 gcgcgactgg cgcctgaagc gcagttccct gccccagaaa tgggtgggat tcgacaatta
    17041 cctcgtggct ttcgaggagc ctgagttctg gaatgccgtg aaggtcacca cgaccttcgt
    17101 gatactcgat gtgctcctga cggtttgcct cgcgctggcc gtggcgctgc tgctgcggcg
    17161 cgccggcgtg ctgcagagcc tcgtgcgcgc cgtgattatc ctgccgtttg ccgtaagccc
    17221 tgcgctgatc ggcgtatcct tccgcttcat gctcaacggc gagcatgggg tgctggcccg
    17281 cggtttcgcg gcgactttcc cctggctgtc ggatgtggtc tggctggcga cgccgggcta
    17341 cgcgatggcc gcgctgatcg cctccgatgt ctggcactgg ttcccctacc tgacgctgat
    17401 gttcctgggc ggccttgccg caattccgaa ggagacggag gaggccgccg aactggacgg
    17461 ggcgaatggc ttgcagatga tcttcctggt cattctgccg cagattcgtt ccatcatcgc
    17521 ggtcggggcc atcctgaaga cgatcttcgc gctgaagatg ttcgatcagg tcgcgaccct
    17581 aaccggcggc ggacccggca tcaccacgca gacgctggcg cattacacct atgcggtcgg
    17641 cttcgtgcac tacgacatgg gccttgcctc ggcgcttgcg gtgatcctca ccgcgatcct
    17701 tgtgacgatc ggcatattct acatccggct ggtccttccg cagcgggagc acagcgcgcc
    17761 atgagcgatg caccccggcc gcccgtcctc gtgtcggctg cgctgatcct gctgttcggg
    17821 atcatcgttt ttccgattgc ctggatggtc ctgacggcct tcaagcagcc acgggacgtc
    17881 tattcgctga ccttcgtctt cgcgccgacg ctggagaact ttatcacggt cttccagccg
    17941 ccctggaaca tcggccgtcg catcgtcaac agcctgctga ttgctggggg cacgctggcg
    18001 atcgcgatcc cggcggcgat cctggcggcc tattcgttct cgcgcttcca gttcatgttc
    18061 aaacgcagcc tgtttttcct cgtgctggca acgcagttcg ttcccgccgt ggttgtcatc
    18121 ctgccgttct acctgatgtt ccgcgatctc gggctgctgg acacgcgcat cggcctgatc
    18181 atcgtcaatc tggccatcgt gacgcctttc gccatctgga tgctgaaggg cttcttcgac
    18241 gcggtgccgg tcgagtgcga cgaggcggcg ctgatcgacg gcgcatcacg gctcgacatc
    18301 atccgcatgg tgatagtgcc gatggcgatg ccgggcatca tcgtcgcctc ggtcttctgt
    18361 tttatcctga catggaacga gttcatcttc gcgctgatcc tggcccggga caatgcggtg
    18421 acgctgcagg tcggtctggt caacttccgc accgagcgcg gcgacctgtg ggaactgatg
    18481 gcggcggccg gggtcttcat cacggttccc gccttcattg cagcgctctc gatccagaag
    18541 cacttcgagg ccggattgac gggaggcgcc gtgaaatgaa ccgagaactc gatccgaagg
    18601 tcttcgccgc gctcagggcg gtcgatacgc ccaccatttg caacgccatc gaggcgatcg
    18661 aaggccgccg gcgggctgat ggcttcacgc agtcgcccgt ggtctgcgcc gatgtgaacc
    18721 tcccggcgat ggtcggattc gcgaagaccg cggttatccg ttcggaagct ccggcaagcc
    18781 ggcccgccgc cgagatgcgc gacatacgcc tcaactacta tcgctacgtc gcggccggtg
    18841 gaccgcatcg gctggtcgtt atcgaggatc gcggcggcag gaagggcgtc ggcgccttct
    18901 ggggcgaggt caacgtcgcg ctgcacaagg ggctcggcct tcagggcgtg ctgacggacg
    18961 gttccatccg tgatctcggc gccatcgatg ccggcttcca gctgctggcc ggcagtgtcg
    19021 ggccaagcca tgccttcgtg catgtcgccg aattcgatac gcccgtcacc gtcttcggac
    19081 tgctggtgaa accgggcgat ctcatccatg ccgaccgcca tggcgcggtc gtcatcgagc
    19141 cgggcatcga gaaagatctg ccacgcgcca tcgacctggt gacgcgaaag gagcagccgc
    19201 tgctgaaagc ggcgcgagcg cccgacttca acatcgacaa gctgctctcc gcctgggggg
    19261 aggcggagga tgtccactga aatcaagctt tccaagaccc tgagaaggag ccccccatgg
    19321 tcgacatgat gagccgcatc gtccgttacg gcgaattgaa gccctgcaag accgccttca
    19381 tcgatgccca tacgccggga tcgaaccaga aggagaactt caccatcatc ggcgccggcg
    19441 tctcggaatc agctgaccag catgtgcata tccgcgagac acccggcttc aacatcggag
    19501 cggcgggcca accgccgaaa tgccggaact cgctgcattc gcaccgcacc gcggaggtgt
    19561 tcttcgttct gaagggtcgc tggcgcttct tctggggccg ctggggcacg gctggcgagg
    19621 tgatcctgga agagggcgac atcttcaaca ttccgacagg cattttccgg ggcttcgaaa
    19681 acatcggcac cgactacggg atgatcatgg ccatcctcgg cggtgacgat gccggcggcg
    19741 gcgtcatctg ggcgccgcag gtcatcgagg atgcgaaggc gcacgggcta gtgctcggtg
    19801 ccaacggccg tctctacgac accaagaagg gtcaggccct gcccaagggc gtgaagccga
    19861 tgccgctcct gaccgacaag gagctcaagg ccttccccga ggtgccggtc gagggagtcg
    19921 tgcgcaacta cgtcgcccgc tactgggacc tcatggccct ggcggccggc aagccctgca
    19981 aggtcatcgg cgccgaggcc gtgctgaagg accgtcccgg attcgaggtc gagctcctga
    20041 cccggggctc catctccgaa aagccctatt cgatgaagcg gcatgagatc ctgatgccgg
    20101 tgcgcggtca ctggcggctt gcctgggagg gcggtgagac gatcctcaat ccgggcgaca
    20161 cctgcgctgt tccgcctggc ctcaagcaca gccttgtgcc gtccatgacc ggtgagtgca
    20221 gcctctatcg ggtgacgaac accaaggaca aggcgggacc gacccgcgtc atcaactgag
    20281 gctaaccatg acgatccaga cgacgcatgt cggctccctg ccgcgccccc agaaggttgc
    20341 ggacctgctg ttcggccgcg aaaaagggct gcctttcgac gagaagccat atgacgccgt
    20401 gatgaaagag gcgacggtcg cgatcgtcgc cgcccagaag gcggcgatgg tggacattcc
    20461 ctccgacggc gagacctcga aaatctccta cgccacctac atcaaggacc ggctgacggg
    20521 attcgatggg gattcgccgc gccgcccgcc gcaggatctt gacgattacc cgacctacag
    20581 ggatcggctg gcgcgggcag gggggacgcc gagctaccgc aggccgaaat gcgtcggtcc
    20641 gatcaagctc aagacgcttg atcccctgaa aaaggacatc gccgccatga aggcggcgat
    20701 gaagaaggcg ggataccgga aaggtttcat gaatgccgcc agccccggcg tcatcgcgct
    20761 gttccagccc tcagactatc acaaggacat ggacgcctat ctcggcgacc tctccgacgc
    20821 gatgcggcac gaatacgaag ccatcgtcga ggccggcctg atcctccaga tcgatgcgcc
    20881 cgatctcggc ctcggccgcc atatgatgta ccgcgaactg gacgagaaga agttcctcaa
    20941 gcgcttcgcc gtgcatgtcg aggcgatcaa caatgcgctg cgaaacgtgc cgcgtgaccg
    21001 cgtccgcatg catatctgct ggggcaacta cgaaggaccg cacacccggg acatcgcgct
    21061 caaggcggtg cttcccggac tgctgaagtc caaacccggc gccctgctgt tcgaaggcgc
    21121 caacccgcgc catgcgcacg agtggacggt ctggcgcgag agaaagctgc cggatgacct
    21181 cgtcctgatc ccgggttgca tcgattccac gacgaatttc gtcgagcacc cggaactggt
    21241 cgcgcagcgg cttgccaatt acatcggcgc tgttggtgtc gaccgggtga tcgccggaac
    21301 cgactgcggc ttctcgacct tcgccggctt cggcgtggtc gaaccggaga tcacctgggc
    21361 gaagctcagg agcctgaagg aaggcgccga gatcgcggcg acccgcgccc gctaggtggc
    21421 gagcgtgctg ccgctcgtgc tcattcccgg gatgatgtgc gacgcccgca tgtggggcgc
    21481 gccgggcgaa ctcggtcggc ggatggtcgt gcacgccgct tgcaccgagt acgacacgat
    21541 gccggcgctg gcctcggcga tcctggcgga tgcgccccgg cgcttcgctt tggctggcct
    21601 gtcgatgggt ggcatcgtcg cgatggagat gcttcgccag gcgcccgagc gcatcgaacg
    21661 tctggcgctg ctggatacca accctttcgc cgagcagccg gaagcgcgcg cccgccgtca
    21721 ggggcagatc gaccgcgcat tgggcggcgc gctcgccagc gtcatgcgcg acgagatgaa
    21781 gccgaactat ctcgcgcccg gacctggcaa tatccctgtg ctcgatctgt gcctggcgat
    21841 ggcgctggcg cttggtcccg aggtcttcgc ccggcagtcg cgggccttgc gcgatcgatc
    21901 cgaccaatcc gaaacgctgc ggagcttcag ggggccggcc ctcgtgcttt tggggcgtca
    21961 cgacagcctt tgccctttgc agcgccatga agcgatgcag gcgatgatgc cgcaggcgcg
    22021 cctcgctgtc atcgagggcg ccggccatct gccgacactc gagcaaccag cggcgacctg
    22081 ggcggaactg acacgctggc tggaggcatg acgggccggg gcttcgccgt tcggcgcgag
    22141 gggcgttcgc cacgcattgc gtgatgcggt tcatggatgg cggcgcgcat gcgtcgcttg
    22201 tatgcggagg acgacaggca tggcgactga catcgagatc gcgcgcgagg ccaggttgaa
    22261 gccgattgcc gccatcgcgg caaagatcgg tgttcccgag agcgccgtgt ctccctatgg
    22321 ccggcacatc ggcaagatcg acctcgccca tgtcgaaacg ctcggcgaca ggcccacagg
    22381 caagctcatc cttgtcacgg cgatcagccc gacgccggcc ggcgagggca agacgaccac
    22441 gacggtcggt cttggcgatg gtttgggccg gatcggccgc aaggcgatga tctgcctgcg
    22501 agagccgtcg ctgggcccct gcttcggcgt caagggcggt gctgccggcg gcggccacgc
    22561 ccaggtcgtg ccgatggagc agatcaacct gcatttcacc ggcgatttcc atgccatcgg
    22621 ctcggcgcac aacctgctct cggccatgat cgaaaaccat atctactggg gcaacgcgct
    22681 gcagttcgat acgcggcggg tcgcctggcg ccgggccatc gacatgaacg accggtcgct
    22741 gcggtcgatc gtctcgtccc ttggtggcgc tacgaatggc tttccgcgcg aggatggatt
    22801 tgacatcacg gtcgcctctg aggtcatggc cgtcttctgt ctggccacag gactgcgcga
    22861 ccttgaggag cggctgggcc gcatggtggt tggccacacc cgcgaccgca cggcgatcct
    22921 ggcgcgcgac gtcaaggcgg cgggcgccat gacggtcctg ctcaaggacg cgctgtcccc
    22981 gaacctcgtg cagaccctgg agggcaatcc ggccttcgtc catggcgggc cgttcggcaa
    23041 catcgcgcat ggctgcaact cggtgattgc gaccggagcc gcattgaggc ttgccgacta
    23101 cgtggtgacg gaggcggggt tcggggccga tctcggcgcc gagaagttct tcaacatcaa
    23161 gtgccgctcc gcgggcttca cgccggccgc cgccgtgatc gtcgccacgg tgcgggcgct
    23221 caagatgcat ggcggggtgc agaaggacgc cctgggcggc gaagacctca aggcgctcgc
    23281 aaagggactt tcgaacctgc gccggcatgt gcagaacgtg cgcaagttcg gcgttcccgc
    23341 cgtggtggca atcaaccatt tcacctcgga cactccggcg gaacaccggc tgatccagga
    23401 gtattgccgc acagaactgg gcgtggaggc cgtcgtctgc agccactggg cggacggctc
    23461 gcgcggaacg gaggaactgg cgcacaaggt cgtcgccctg gccgagacgc cggcgccgct
    23521 ggacgggcgc gggttccggc ccctctaccc cctcgacatg ccgctatggg acaagatgcg
    23581 gacgatcgcg accgagattt acggcgcgtc ggatatcatc gcggacaaga aggtgcgcga
    23641 ccagttcagg acgctggaag cagaaggcta tgggaatctg ccgatctgcg tcgccaagac
    23701 ccagtattcg ttctccaccg atcccgccct gcgcggggcg ccatccggtc acatcgtgcc
    23761 gatccgcgaa atcacgcttt cggcgggcgc cggattcctc gtggtcgtct gcggcgagat
    23821 catgcgcatg ccggggctgc cgcgcgtgcc ttcggcagag aaaatccaca tcgatcagca
    23881 ggggcggatt gaaggattgt tctagggtca gggcgattcc tcgtcgagca gccggtcggc
    23941 cggccggtcg acggcggcgc ggtgatcgcc tataggttcc atgtccggtc gcggggaggg
    24001 cgcagccgat tggattggcg gctacctaag tcgcggaagg aagtgctgga gtgtcgttat
    24061 caaccgccag ggtggcgtcc ggccttgcga aggactatcg cccggaagtc gacggattgc
    24121 gggccgtcgc cgtgctggcg gtcattgtct atcatgccga acttccctgg ttgcggggcg
    24181 gctttgccgg cgtcgatgtc ttcttcgttg tcagcggcta cctgatcacc cggctcatcc
    24241 tggcggaacg ggatgcaggc cattttagcc tgctgcggtt tttcgaacgg cgcgcgcggc
    24301 gcattctgcc ggcgctttat ctcgtgatcg ccgcctgcct gcctttcgcc tggatgctga
    24361 tgtttcccga agagcttcgg cgcttcggcg acagcgttct ttcggttctg ctgctggtcg
    24421 cgaacttcta tttcgtcaac acgcaggatt acttcgcccc cggcgccgaa ctttcccccc
    24481 tgctccacgc atggagcctc tcggcggagg agcaatttta cctggtcttc ccgatcctcg
    24541 cgctggcgac cctgtcgatt tcacgcagag cctttgtcgg ctgcatcctc tgcctggcga
    24601 cggcgagcct tggccttgcc cagttcggcg ggcttctcca tgagaacgcc ggcggattgc
    24661 gttggttggc tccgccggca tggggctact acctgctgcc gacgcgggcc tgggagcttc
    24721 tgctcggcgc agcgcttgcg gctggccgct tcgaacagac tgccttgaac gcgcgcctgc
    24781 ccggtctgct ccgccagact ctcggcattg ccggcatcgc gttgatcgtc gcgtcatgtc
    24841 ttttctacag gcgcagcacg ccgtttccca cggcctacgc gctggttcca acgatcggaa
    24901 cagcactcgt catcctgttc gctcgacaag gcacgcttgc gcatgcggcc cttgcctggc
    24961 gcccgctcgt cggtatcggg ctcatgagct acagccttta tctctggcat cagcccgtgc
    25021 tggcgttctc ccggctgtat ctgccgcgcg gaatgagcga ggtctggctc gtcggcgctg
    25081 tcgcgctgag tttcctgctg gcctggctag gctggcgttt tgtcgagcag ccttgccgcg
    25141 accgtgccgc cgtttcgacg cgcgcctttg cccttggcgt gatcctgagc ggtcttgccc
    25201 tgggcgcggc aggctatgcg tgccgggtcg taggcgccga tcccatgctg ctgtcgcgag
    25261 ccggggcgat ttctgcgcgt gtcgatcacg cggtggggct gtccccggcc tgcgtctatt
    25321 tcggctcctg gcatcataag ccggagtgcg cgaccggcga cgaaccggag gccattgtct
    25381 ggggcgattc cttcgccgcg cataccgtga acgcgatcgt cgcggccaac cccggtctgc
    25441 ctctcttgca ggccacaatg ccggtttgcg gcccgattcc gggaattgcg ccggttgtga
    25501 acgagtatga cgagtcctgg ggacgccggt gcatccagtt caaccaggcg gtgctcgaac
    25561 atgttcttgc cagcccgagc atccgggagg tcatccttgg cattcatttt gccaactaca
    25621 tctggggtga acagaagttg tttgacggga cgcgcgtccg ttccgcctcg cccgagaggc
    25681 tgcgaggcga actgcgcgcg gccatcgggc ggctgcgcgc tgccggcaag cgcgtcacgc
    25741 tgatctcatc gccggcgcgc agcctttttg atgtcggtcg ctgcctggct ctcgccacct
    25801 ggcgttccct gcccggagaa atctgtgact tctcgtacag cgatcatccg caaaacgcct
    25861 tcatcgatgc cttcctgcgt tcgctcgaag gcgagggcgt ccacgtcatt gcgctgcg
//
LOCUS       DAOMXN010000014        25406 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_1228_length_25406_cov_12.135537, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000014 DAOMXN010000000
VERSION     DAOMXN010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 25406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25406
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1228_length_25406_cov_12.135537"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="PK812_02715"
     CDS             <1..243
                     /locus_tag="PK812_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRJ68494.1"
                     /translation="LHPMQEAFRDNHGLQCGFCTPGMIMTAVDMVNRLGHDLDEHTVR
                     DELEGNICRCTGYHNIVKSVQAGAKAMGKGKKKKAA"
     gene            312..2678
                     /locus_tag="PK812_02720"
     CDS             312..2678
                     /locus_tag="PK812_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HRJ68495.1"
                     /translation="MSATGVGASVRRKEDFRFITGNGHYTDDINRPGQAHAYFLRSPH
                     AHATIKKIDTSAAAKTPGVVAIFTGADIEADKVGGLICGWMIHNKDGSPMKAGAHPAL
                     AQGKVRYVGDHVAVVIADSLAIARDAAEKIKVDYGVLPAVADLSKATDKGAATVHDVS
                     ANNQVYDWHLGDKAATDKAFKAAKHVTKLDFSNNRLIPNAMEPRAAVGEYDTGTDSLT
                     LYTTSQNPHVTRLVMSAFLGLAPEHKLRVIAPDVGGGFGSKIFIYAEETVCIWAARKI
                     KRPVKWTADRTEAFLADAHGRDHITHAEMAVDDKGKITGVRVKTRANLGAYLSTFASS
                     VPTYLYAPLLSGQYDIPAIYCEVEGVYTTTAPVDAYRGAGRPEATYVVERLVEVTARQ
                     LGQDPAAFRKRNFIKKFPHQTPVIMCYDAGDYAASLNKALDLADYKGFGKRKRDSARK
                     GMLRGIGFSAYIEACGIAPSQAVGSLGAGVGLWESAEVRVNPVGTVEVLTGSHSHGQG
                     HETTFAQLVSDRLGISIDNVSIVHGDTDKVQFGMGTYGSRSGAVGMTAIAKALDKVIA
                     KGKKVAAFVLEAAEGDIDFKDGHFSVKGTDKKIDFGSVALQAYIAHKFTGAQLEPGLK
                     EGAFYDPTNFTFPSGVHICEVEVDPATGRTRIDRWTAVDDFGNVINPMIVEGQVHGGI
                     AQGVGQALLEGAVYDSSGQLLTASYMDYCMPRADDLPSYTVGTTVTACPSNPLGIKGC
                     GEAGAIAAPPAVINAITDAVGHEDVAMPATPQAVWRAVQKGMKKQAAE"
     gene            2682..3155
                     /locus_tag="PK812_02725"
     CDS             2682..3155
                     /locus_tag="PK812_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68496.1"
                     /translation="MRGTLPAVFAVLACASLEAQAQHRWTTSIKQGIEEHVIRTGGGA
                     RFIISCPSGLEKTNPGFTFESKKLKIAQSETRSVELILGIDAASHAYKVECSGDSCDW
                     TSADAAGQSAIEKSVNSLRSAKKFVVRVPGLKVSEEFSTSGARKALENALDGCES"
     gene            3169..3972
                     /locus_tag="PK812_02730"
     CDS             3169..3972
                     /locus_tag="PK812_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018261279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HRJ68497.1"
                     /translation="MYAFKYHRPASLRKAAGLLAKHEDAKLLAGGHTLLPTMKNRLAQ
                     PGHIVDLGALTDLAGIQKKGRNIVIGAMTRHADVAGNADVKAALPGLAALAGGIGDPA
                     VRHRGTIGGSIANNDPAADYPAACLALGATIVTSKRKIPADEFFNGLFTTALEEGEII
                     TKISFPIAGKFAYAKFPNPASRYAMVGVAVAKKGGDVRVAVTGAGADGVFRWAKAEEA
                     LKGRFNAKALDGVVHPAKGINGDIHGSAEYRAHLIGVMAKRAVAAATGK"
     gene            complement(4119..4958)
                     /gene="bcsS"
                     /locus_tag="PK812_02735"
     CDS             complement(4119..4958)
                     /gene="bcsS"
                     /locus_tag="PK812_02735"
                     /inference="COORDINATES: protein motif:HMM:NF028346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsS"
                     /protein_id="HRJ68498.1"
                     /translation="MRHRAMAVALAGLVLASVGPAQALDDVSLTYGNYWRVAFSSVEI
                     EKASAFLSSGAKLGVSNPTNDVALFIASSQGYSLSDGERAFRLGWPANRVETQSRMMV
                     GLERSEGKVFVSLAAGVSQARMQAMRQPDPNLRLVQLARQYDVDLRLLPVLLMSVPPS
                     TIRGLLPPHMKDHAGAVVDLNVWWRPDNKTYLHWAVVGDSAAQSLWTRLRAGAKLGAL
                     PFSFGPEAATAVGRNWHKYKVGLHLGDLKLWRFELAAAGGWIYDETRRWGTYGSLALH
                     TRF"
     gene            5117..6172
                     /locus_tag="PK812_02740"
     CDS             5117..6172
                     /locus_tag="PK812_02740"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRJ68499.1"
                     /translation="MNQILSVQVLRALAALSVAYGHAAYDASQIAARSGQTFEPAFRE
                     LSAAGVDLFFVISGFVMVWSSRSLFGRPGAAGLFIGRRLARIVPLYWATTTVFLLALA
                     MLPQAVNAAAPAVGDIVKSYVFIPFRRPGDGSLQPVYGLGWTLNYEMFFYVCFAGLLA
                     LRAGWAIGVLVAVFSGIVAAGYWLTPQSAPLQFWSQPIILEFVLGALVGYAYLSGVRV
                     PRPVGLAAVALAACFFLAATGGTFPQGAFDRLWMLGAPAALALAGATLVNQSPREGAV
                     WRGLAMLGDASYALYLIHPMLIRALNMVWTKLGLAPQVGWPFIIVALLLSLASAVVVY
                     RWFERPLTLWLQGRSRS"
     gene            6121..6909
                     /locus_tag="PK812_02745"
     CDS             6121..6909
                     /locus_tag="PK812_02745"
                     /inference="COORDINATES: protein motif:HMM:NF017833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HRJ68500.1"
                     /translation="MVRAAADALAAGTITVVTALPGREAKGTGAPAFSLAGRTGKRSA
                     RRYIVAASSRYLRVPLQQPKTISEVLTALLAEGPTSEDGKMSLDEMVGALKHRAFGIL
                     YLLFGLPNCLPMPPGIPILCGVVVAVISLQMLLGQPVLVLPRWLGKRRVSRAMLTGVV
                     EKSAKYLTALERFSRPRFPLLSGDLMRRAIGLAGLALAVALMAPIPLFGGIPAGIAVT
                     ILGLAFTQRDGVLIGFGVLIATPVALAVTSAMLFALFQGAAAMF"
     gene            complement(6928..9036)
                     /locus_tag="PK812_02750"
     CDS             complement(6928..9036)
                     /locus_tag="PK812_02750"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRJ68501.1"
                     /translation="MSLAPDVGHRTQSVSLSIVPILVLTFAVIGSMSFLARSAFETNE
                     QRAVALEQRRIELALEEKARDFANSIEAELQLLFREDSTPKTVVEYQKAIDYLADAHD
                     IEFAAVFEKTGQLVAAHVRSPGILERRMKDAGEIIAVRRDQKTEYALRRLGMSRIVIE
                     PSTIKALAAVNVAGNYELVASKSLGEPVLAEIGNALMLDGLRFRHPEAQSAPDGTGFA
                     MIAWNRINDGSITLDSLASIGFLGIAVIGIYGLLVYMHIRRVTRELEHNEAKAQHLAG
                     HDPLSGLPNRLLFSKRLDHELRRLQRDGGGLAVMYLDLDKFKEVNDKLGHDAGDKLIG
                     MVARRLGDLVRGADMVARFGGDEFAIIQVGVRSISDTEVLARRILGELQRPFRIDDAD
                     AQVGVSIGISVAPDNGSDAPALMRYADVALYRAKNEGKNRFSFFEQEMNEQLRIKKLV
                     EDDLRNAIDRGELELNYQPIVTPEGGKIVGLEGLVRWNHPVRGRIAPNQFIGIAEERG
                     LICPLGEWVLRRACEDGKRWPDLTIAVNVSPVQFKQKTFVETVSKIIRETGFDAARLE
                     LELTEGVVIEDADAAEAAIMELRTMGVRLALDDFGVGYSSLIYLRRFAFDRIKIDRSF
                     LESMEAAGESAVLLHSIIHLGRALGLQVTAEGVETEEQRRFLQAVGCHHLQGYYFAKP
                     TTADEIDLIIRDINGQRTAA"
     gene            complement(9173..9856)
                     /gene="radC"
                     /locus_tag="PK812_02755"
     CDS             complement(9173..9856)
                     /gene="radC"
                     /locus_tag="PK812_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HRJ68502.1"
                     /translation="MTDETPHYHGHRDRLRQRFTTTGGDGLADYELLELVLFRSIPRR
                     DVKALAKDLIARFGSFAEVVAAPTERLREVGGMGEATAIDLKIIEAASRRMAKGAVRK
                     RESLSSWRAVIDYCRVSMAFSEREELRLLFLDKRNGLIADEVQQTGTVDHTPVYPREV
                     VRRALELSATALIIVHNHPSGDPTPSSADIKMTQDIVAIAKPLGLTIHDHIIIGRDGH
                     ASFKGLGLL"
     gene            complement(9853..10674)
                     /gene="map"
                     /locus_tag="PK812_02760"
     CDS             complement(9853..10674)
                     /gene="map"
                     /locus_tag="PK812_02760"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRJ68503.1"
                     /translation="MNNLEAAPIKRRPGQIRIHGPESFAAMRKAGRLTAEALDMLIDH
                     VRPGVTTDFLDRLVFDFAQDHDALPATLNYRGYTRACCTSINHVVCHGIPDDKPLRDG
                     DIVNIDVTLIVDGWHGDSSRMYLVGDVPRKAQRLVEVTHEALMRGIAAVRPGGTTGDI
                     GHAIQTYAEAERCSVVRDFCGHGLGQLFHDEPNILHFGKPGEGVQLKPGMLFTIEPMI
                     NLGKPHVKVLSDGWTAVTRDRSLSAQFEHTVGVTQDGVEIFTLSPKGLHQPPYGR"
     gene            10815..11654
                     /locus_tag="PK812_02765"
     CDS             10815..11654
                     /locus_tag="PK812_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012317711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HRJ68504.1"
                     /translation="MPDKEPPFGAYAPTGLISRLLGATRALPPQGVSKRIGFVLRRIA
                     ILLLGGRPLDVVSFGVRFRLHPYYNVCEKRILFSPRQFDEIERDLIAQRIRPGFCFVD
                     VGANIGGYALFVAAQAGQGARILAIEPQPEIFRRLMANIGFNPFGTVKAMDCAVADSE
                     GEVTLFIDAENQGESSLKIITAGSRSSVRVPARRLLSILRDEGFDRLDAIKLDVEGAE
                     DLILETFFAKAPEKLYPGLIVIERAPDRWQVDLPSLLVAKGYRLIAETRNNFVFERGF
                     AAD"
     gene            complement(11661..12374)
                     /gene="sfsA"
                     /locus_tag="PK812_02770"
     CDS             complement(11661..12374)
                     /gene="sfsA"
                     /locus_tag="PK812_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HRJ68505.1"
                     /translation="MRFPSRIVPGRLIQRYKRFLAEVALADGSIVTAHCANPGAMLGL
                     TDPGSTVWLSMSPDPKRKLAYNWELVEVDFGDGTQCVGINTSHPNRLAELAISAGTLP
                     ELAGYPTLRREVRYGVNSRIDLLLEAPERAPCYVEVKNVHLCRRAGQAEFPDCVTARG
                     AKHLGELANVVRDGGRAVMLYVIQMRADSFSLAADIDPAYARAFDAARAAGVEALAYS
                     CDVSPDGLGLARAVPFVLG"
     gene            12459..12896
                     /locus_tag="PK812_02775"
     CDS             12459..12896
                     /locus_tag="PK812_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016556419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRJ68506.1"
                     /translation="MPYTLTFHRVFRASPEILYRAFTDAQAKVKWLPPNGFTAKVHHL
                     DLRVGGGYAMSFTSIATGESHSFRGVYDECEPGKRLRYTDRFDDPNLPGAMHVTVDFR
                     AVSVGTDVSIRQDGVPDVIPPEACHVGWQQSLALLALLVEANV"
     gene            12953..13705
                     /locus_tag="PK812_02780"
     CDS             12953..13705
                     /locus_tag="PK812_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018042407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HRJ68507.1"
                     /translation="MTPHPDVTAALLVIGDEILSGRTKDKNIGYIAEYLTGVGIDVRE
                     VRVVPDIEAEIVAAILALKERYTYLFTTGGIGPTHDDITADCVAKAMGVSIDHDPRAV
                     AILRERYRPEDLTEARLRMARIPAGAELIENPISKAPGFMIANVIVMAGVPAIMQAML
                     DNVAGRLKTGARMQSVTIDSGGLPEGIYAEALASVARDHPGVVIGSYPSFTHKGFENK
                     IVLRCKDTSLLAHAEKAVIDALAQLQAAKASR"
     gene            13735..14241
                     /gene="gpt"
                     /locus_tag="PK812_02785"
     CDS             13735..14241
                     /gene="gpt"
                     /locus_tag="PK812_02785"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HRJ68508.1"
                     /translation="MNAQPLPEKAFPVSWDQFHRDARALAWRLAAAGPFRGILCITRG
                     GLVPAAIVARELDVRLIDTVCVASYHDYANQGELKVIKPIDTGLIGEDGGKGVLVVDD
                     LVDTGKTMKLVRDTMPNAHYAAVYAKPLGRSMVETFVTEVSQDTWIYFPWDMGFAFQP
                     PITKGSAG"
     gene            14372..15928
                     /locus_tag="PK812_02790"
     CDS             14372..15928
                     /locus_tag="PK812_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009494101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRJ68509.1"
                     /translation="MFDASRFRKTLPDALLDARDRHGGAKEALEDPERTPLSYDRLIL
                     GALVLGRKLAGEAAAGARVGVLLPNVNGAVVTFFGLLFHGLTPVMLNFTAGLKNLTSA
                     CETAQIGMIVTSKRFVDTAKLDDVVAGLGQGRRVLYLEDVRKQIGVLDKIAGLVRSRF
                     ARAIARRACPDPDAPATILFTSGSEGVPKGVVLTHANIAANAGQIWKLADGMLSEADT
                     AMNPLPIFHSFGLTAGLLLGLFNGMKVVLYPSPLHYKQVPKLAGETRATLLFATDTFL
                     VGYARAADPNDLVSVRLVVTGAEKVKDGTRKLWEPYGATILEGYGCTECSPVLACNTP
                     TANRPGTVGKLLPGIEARLDPVEGIPEGGRLVVKGANVMAGYMLAARPGFVQPPDGGW
                     YDTGDIVDIDAEGFVTIKGRAKRFAKLGGEMVSLAAVEAMASTLWEGATHVAVTLPDA
                     RKGEQVLLVTDRPDADRAALIEFAREQGMPELFVPRAVLVVGAVPVLGSGKVDYAGAA
                     ELARSMRPLI"
     gene            16007..16927
                     /locus_tag="PK812_02795"
     CDS             16007..16927
                     /locus_tag="PK812_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRJ68510.1"
                     /translation="MSSSSFPPVTPSEARRQRLLAIAMTCGAVLCFTLIDTAAKALSP
                     QVGVMTTTWLRYVAAFLFILPFLNPWRVPDLLKSEKPFLQSLRGLLLFGSTILNFVAM
                     QYLQLAQTVSIGFLAPLLFALLAGPFLGEWAGPRRLIAIVIGFMGVLIVARPGLGVIH
                     PAALLSICSATCYSMVLLLTRNLSVRDSAETTMFYSTFVGVVLMAPLAFLNWKTPEFP
                     SGWALVALTGLGGALGHWLMIQAQRRAAAPVLAPFIYFQIVWMSLSGYVFYGEQPDRW
                     TFLGGAIVVASGLYLLHRERKRKPAAGAAS"
     gene            17126..17608
                     /locus_tag="PK812_02800"
     CDS             17126..17608
                     /locus_tag="PK812_02800"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HRJ68511.1"
                     /translation="MAEDGERRQGAIKSPQDLAASLFLLGIAVLALWLGRDLSTGTLR
                     QMGPGMLPKALAVLLAGLAVVLLIDAFKEAGEELAAWSPRAIIFVLGAFCAFGFAVRP
                     LGLSVAGPLAVIIAGFASNETKFVETLIFGLVMTAFCVGLFKFALGLPIPLAPWLIGY
                     "
     gene            17619..19121
                     /locus_tag="PK812_02805"
     CDS             17619..19121
                     /locus_tag="PK812_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007773183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRJ68512.1"
                     /translation="MDIIQNLGLGFSVALTFQNAALCFIGCLVGTMIGVLPGVGPIAT
                     IAMLLPLTFKVDPTGALIMLAGIYYGAQYGGSTTAILINIPGEATSVVTALDGHMMAK
                     NGRAGVALGMAAIGSFIAGTIATLVIALLGKPLTAMALIFGPAEYFSLMVLGLALAVA
                     LARGSVLKAILMIVLGILLSTVGTDLETGEERLTLGLQDLSDGIDFAVLAMGVFGLAE
                     ILKNLENPEARDVVKSKIGRLLPSMAEFRQSLAPILRGTSLGSILGILPGNGAVLAPF
                     ASYTIEKKIAKDPSRFGKGAIEGVAGPEAANNAGAQTAFIPLLTLGIPPNAVMALMVG
                     AMTIHGIIPGPLVMTKSPDLFWGMIASMWIGNLMLVIINLPLIGIWVKLLQVPYRLMY
                     PAILLFCAIGIYSINNNPSDVFFTAFFGLVGYVFYKFGLEPAPMLLGYVLGKLMEQKL
                     RQALTLSRGDFMTFIERPVSGVLLFIALVIIVLALLPSMRKEREQVFAGD"
     gene            19233..20198
                     /locus_tag="PK812_02810"
     CDS             19233..20198
                     /locus_tag="PK812_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HRJ68513.1"
                     /translation="MKRLLLAAAAAVGLAAVPAQAQDYPNKPVTMIVPFAAGGPTDII
                     ARLVAESMSRTLGQQVIIENVAGAGGTTGSTRASQAAADGYTIMMGHMGTHGAAPGLY
                     KNLKYNPSTDFSPIGLAAGTPIIIVAKKAFPADDLKGFMAHVKANNDKITQAHAGVGS
                     VSFTTCMLLTAQLGVKATAVAYTGTGPALNDLVGGQVDYMCDQIPSVVAQIRAGSVKA
                     IAIALDARSPALPNLPTTKEAGLPEFQVSAWNAVFAPKNLPAPIQAKLADALSKALDD
                     DKVKARLLDLGAVIPQGDGRGPDALAKLVKSEVERWGPILKAVAQ"
     gene            complement(20268..20585)
                     /locus_tag="PK812_02815"
     CDS             complement(20268..20585)
                     /locus_tag="PK812_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68514.1"
                     /translation="MKFKILVPVAIVAAGLMGVSGEAQAKDGNRKSALIGAAAGVAVG
                     AVGATLYHRSQQPSAPARPEYTGSTARPVSYQAAGGCSVRPVRLFDEQGNYVKTERLK
                     VCR"
     gene            20575..20907
                     /locus_tag="PK812_02820"
     CDS             20575..20907
                     /locus_tag="PK812_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68515.1"
                     /translation="MNFMIVTRLIAPPPGCAARVNTTWAALAALADPVPTFRSKCCEW
                     LKVIETGLIAALAYFKNVNILNINIKERAWRGAFLSFWGIPQSRAFAVLWRKAMWPAR
                     EALATMRE"
     gene            20793..21377
                     /locus_tag="PK812_02825"
     CDS             20793..21377
                     /locus_tag="PK812_02825"
                     /EC_number="1.-.-.-"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRJ68516.1"
                     /translation="MARRILVILGHPAEQSFCRALAESYVAGAGSAGHYARVIDLRAL
                     TFDPVLHGGYGGEQSLESDLVRAQESILWAQHLVFVYPIWWGAVPALLKGFIDRVFLP
                     GFAFKYHKDKISTWDKLLSGRTARLLVTMDSPPWYFRFVSRAPGHNQMRRTILEFCGV
                     KPVAISSFGSVQASDAARRAAWLKQAEGLGSAGS"
     gene            21475..22518
                     /locus_tag="PK812_02830"
     CDS             21475..22518
                     /locus_tag="PK812_02830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRJ68517.1"
                     /translation="MTASLELGLDTFGDVTADEAGRPVSHAQVIRDLVDEAVLADQVG
                     LDFIGVGEHHRDDFAVSAPEAVLAAMAVKTRRIHLGSAVTVLSSDDPIRVFQRFATVD
                     ALSNGRAEVILGRGSFTESFPLFGFNLRDYEVLFEDKLDLFARLASNEAVTWQSPASP
                     PTRTPLKNQHVYPKLEHGPLKVWIGVGGSPESVVRAARYGLPLMLAIIGGEPARFKPF
                     VDLYGRACEQLGQPRLPIGVHSPGYVADTDAAAREEFFQDYKAMRDRIGRERGWPPMG
                     RGEFDHEVEKGSLYLGSPETVAKKIAKTARALGISRFDLKYSAGPLPHARLMRCIELY
                     GTKVMPMVREMLA"
     gene            complement(22522..22608)
                     /locus_tag="PK812_02835"
     tRNA            complement(22522..22608)
                     /locus_tag="PK812_02835"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22572..22574),aa:Leu,seq:taa)
     gene            complement(22656..23435)
                     /locus_tag="PK812_02840"
     CDS             complement(22656..23435)
                     /locus_tag="PK812_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRJ68518.1"
                     /translation="MDLGLKGRKAIVCAASKGLGRGCAEALAAEGVDLVICARTRADI
                     ESAAAEIAAKFGVKVTPVACDVTTEEGRKAVLAACPDPDILVTNAAGPPAGDFRNFTL
                     DDWRKAAEGNFFTPIALIQATVYGMMERGFGRIVNITSASVKAPIAMLELSNGVRAGL
                     TGAVATLARTVSKHNVTINNLLPGQHDTDRLTAIIGNNMKKSGKDRDATVSEMQARIP
                     ANRFGTAEEFGHACAFLCSAKSGFITGQNLVADGGAYPGTL"
     gene            complement(23586..24095)
                     /locus_tag="PK812_02845"
     CDS             complement(23586..24095)
                     /locus_tag="PK812_02845"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HRJ68519.1"
                     /translation="MNIPVVRTAVEPRYKYISTKTYRQIGPVAYRQWRADSHCKFVHG
                     YALSFHFEFGCKDLDARNWCMDFGGLKPLKELLDEWFDHKLLVAADDPHREELLNLGK
                     LGIAQITEVEKTGCEGLADFLYEYINTIFLKQYGEQDRIWCCKVEVRETDSNMAMRVG
                     HREDNEFVD"
     gene            complement(24193..25374)
                     /locus_tag="PK812_02850"
     CDS             complement(24193..25374)
                     /locus_tag="PK812_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HRJ68520.1"
                     /translation="MSGGKSAVIVGAGIGGLTAALSLARDGWQVQVFEQAHRLEPVGS
                     GLQISPNASRVLADLGVLPRLDGLAVEPEAMVVRSGRNGSTLTSMPLGETAEKRWGAP
                     FLVVHRGDLQNALLAAATEQSSIALHLGRRLEALKFRDRSVTATLSRYTDELTVETDL
                     LIGADGLWSKVRGQVGLTGPASFSGCVAWRTLIPADSVPAFAREMRSNLWLGSGAHVV
                     HYPLRGGSVINVVAVVEENWRERGWSEPGDIAWINRRFEDWHSDLTALIGSADTWLRW
                     SLFDRAPEWRWSRSPAVLLGDAAHPMLPFLAQGAAQAIEDAAVLSRSLRGHASISDAL
                     KGYEEERLRRAARIQRLSHRQASIYHLANPAAIVRDAAMALLGHDVLARRYGWIYDHR
                     A"
BASE COUNT         4405 a         8045 c         8170 g         4786 t
ORIGIN      
        1 ctgcatccga tgcaggaggc gttccgcgac aatcacggcc tgcagtgcgg cttctgcacc
       61 ccgggcatga tcatgacggc cgtcgacatg gtcaaccggc tcggccacga cctcgacgaa
      121 cacacggttc gcgacgaatt ggaaggcaat atctgccgct gcacgggcta ccataacatc
      181 gtcaagtcgg ttcaggccgg cgccaaggcg atgggcaagg gcaagaagaa aaaggcggcc
      241 tgaccgaagc cgcctcgctc acgtttgatg ccaagaatgc aatccaattc gagttccttg
      301 ggaggaacgc catgagtgcc accggagtcg gcgcatctgt ccgacgcaaa gaggacttcc
      361 gcttcatcac gggcaacggc cactacaccg acgacatcaa ccgtcccggc caggcccatg
      421 cctatttcct gcgctcgccg cacgcccatg cgacgatcaa gaagatcgac acgtccgcgg
      481 ctgccaagac ccccggcgtc gtggcgatct tcaccggcgc ggacatcgag gccgacaagg
      541 tgggcgggct gatctgcggc tggatgatcc acaacaagga cggatcgccc atgaaggccg
      601 gcgcgcatcc ggcgctggcg cagggcaagg tgcgctatgt cggcgaccat gtcgcggtcg
      661 tcatcgccga ttctctggcg attgcgcggg acgcagcgga gaagatcaag gtcgactacg
      721 gcgttctgcc ggcggtggcc gatctttcca aggcgacgga caagggcgcc gcgactgtcc
      781 atgacgtgtc ggccaacaac caggtctatg actggcacct cggcgacaag gccgcgaccg
      841 acaaggcctt caaggctgcc aagcatgtca ccaaactgga cttttcgaac aaccggctga
      901 tccccaatgc gatggagccg cgcgcggccg ttggcgagta tgacaccggt acggacagcc
      961 tgacgctcta cacgaccagc cagaatccgc atgtgacacg cctcgtcatg agcgctttcc
     1021 tcgggctcgc accggagcac aagctgcgcg tgatcgcgcc ggacgtcggc ggcggcttcg
     1081 gctcgaagat cttcatctac gcggaagaga cggtctgcat ctgggcggcg cgcaagatca
     1141 agcgcccggt caagtggacg gcggaccgca cggaagcctt ccttgccgac gcccatggcc
     1201 gcgaccacat cacgcatgcc gagatggccg tggacgacaa gggcaagatt accggcgtgc
     1261 gggtgaagac gcgcgccaat ctcggcgcgt atctctccac cttcgcctcg tcggtgccga
     1321 cctatctcta cgcgccgttg ctgtccggac agtacgacat cccggcaatc tactgcgagg
     1381 tcgagggcgt ctataccacg acggcgccgg tcgacgccta tcgcggcgct gggcgccccg
     1441 aggcgaccta tgtggtggaa cggctcgtcg aggtgacggc gcgccagctg gggcaggacc
     1501 cggctgcctt ccgcaaacgc aacttcatca agaagttccc gcaccagacg ccggtgatca
     1561 tgtgttacga cgcgggcgat tatgccgcct cgttgaacaa ggcgctcgac ctggccgact
     1621 acaagggctt cggcaagcgc aagcgcgaca gcgcccgcaa gggcatgctg cgcggcatcg
     1681 gcttctccgc ctatatcgaa gcgtgcggca tcgcgccctc gcaggcggtc ggttctctcg
     1741 gcgccggcgt cgggctgtgg gaatcggcgg aagtgcgggt caacccggtc ggcaccgtcg
     1801 aggtgctgac gggttcgcac agccatggcc aggggcacga gacgaccttc gcgcagcttg
     1861 tcagcgaccg cctgggcatc tcgatcgaca acgtgtcgat cgtccacggc gacaccgaca
     1921 aggtgcagtt cggcatgggc acatacggct cgcgctccgg cgcggtcggc atgaccgcaa
     1981 tcgccaaggc gctcgacaag gtgatcgcca agggcaagaa ggtcgcggcc ttcgtgcttg
     2041 aagcggcgga aggcgacatc gacttcaagg atgggcactt cagcgtgaag ggcaccgaca
     2101 agaagatcga cttcgggtcg gtggcgctgc aggcctatat cgcccacaag ttcacgggcg
     2161 cgcagctgga gccgggcctg aaggaagggg cgttctacga cccgacgaac ttcaccttcc
     2221 cctccggcgt ccacatttgc gaggtcgagg tcgatcccgc gaccgggcgc acccggatcg
     2281 atcgctggac ggcggtcgac gacttcggca acgtcatcaa cccgatgatc gtcgaaggcc
     2341 aggtgcatgg cggcatcgcc cagggcgtcg gtcaggcgct tctggaaggc gcggtctatg
     2401 actcgagcgg acagctgctg acggcgagct acatggacta ttgcatgccg cgcgccgatg
     2461 acctgccatc ctacaccgtt ggcacgactg tcacggcctg cccgtccaat ccgcttggca
     2521 tcaaggggtg cggcgaggcc ggcgcgatcg cggcgccgcc tgccgtgatc aacgcgatca
     2581 cggatgcggt cggtcacgag gacgtggcga tgccggcaac accgcaggcc gtctggcggg
     2641 cagtccagaa gggcatgaag aagcaggcgg cggaataagc catgcgcggc accctgcctg
     2701 cggtcttcgc ggtcctggcc tgcgcctccc tggaggcaca ggcgcagcat cgctggacca
     2761 cgagcatcaa gcaggggatc gaggagcatg tcatccgcac gggcggcggt gcccggttca
     2821 tcatctcctg cccttcgggg ctggagaaga cgaacccggg gttcaccttc gagtcgaaga
     2881 agctgaaaat cgcccagtcc gagacacgtt cggttgagct gattctcggc atcgacgccg
     2941 ccagccacgc ctacaaggtc gagtgttccg gcgattcctg cgactggacc tcggctgacg
     3001 ccgccggcca gagcgcgatc gaaaagtcgg tcaacagcct gcgcagcgcg aaaaagttcg
     3061 tggtccgggt gccgggcctc aaggtttcag aggaattttc cacttcgggc gcacgcaagg
     3121 cgcttgagaa cgcactcgat ggatgcgagt cctagggaga caacccagat gtacgctttc
     3181 aaatatcacc gtcctgcctc gctccggaag gccgccggac tcctagcaaa gcatgaggat
     3241 gccaagctgc tggcgggcgg acacaccctg ttgccgacga tgaagaacag gctggcccag
     3301 cccggacaca tcgtcgatct cggcgctctc accgatctcg ccggtatcca gaaaaaaggc
     3361 cgcaatatcg tcatcggcgc gatgacgcgt catgccgacg tcgccggcaa tgctgacgtg
     3421 aaggcggcgc tgccgggact ggcggcgctg gccggcggta tcggcgatcc ggcggtgcgc
     3481 catcgtggca ccatcggcgg ctcgatcgcc aacaacgatc cggcggcaga ttatcccgct
     3541 gcctgcctgg cgctcggggc gaccatcgtc accagcaagc gcaagatacc agccgacgag
     3601 ttcttcaacg gcctgtttac cacggcgctg gaagagggcg agatcatcac caagatctcg
     3661 tttccgatcg ccggcaagtt cgcctatgcc aagttcccga acccggcctc gcgctatgcc
     3721 atggttggcg tcgcggtggc caagaagggg ggcgacgtgc gcgtcgccgt cacgggcgcc
     3781 ggtgcggatg gtgtcttccg ctgggccaag gccgaggagg cgctgaaagg ccgcttcaac
     3841 gccaaggcgc tggatggcgt cgtgcatccg gcaaagggga tcaacggcga tatccacggc
     3901 tcggccgagt atcgcgcgca tctcattggc gtgatggcga agcgggccgt ggcggccgca
     3961 accgggaagt gacatccagc tgcgctgcgg ccggccatgc cggccgcggt cattccaaca
     4021 ccggaggcgc ctggcttcgt gtcgggcgcc tcgattgttt cggaccgacc gtcggcgatg
     4081 ggcagcggca agggacgacg ccccccacgg gctggcgctc agaagcgcgt gtgcagggca
     4141 agacttccat aggttcccca gcgtcgcgtc tcgtcgtaaa tccagccgcc ggcggccgcc
     4201 agttcgaagc gccagagctt caggtctccc agatgcaggc caaccttgta cttgtgccag
     4261 tttcgaccga ccgcggttgc ggcctcgggt ccgaaggaaa acggcagggc acctagcttc
     4321 gctccggcgc gaagtcgggt ccacaggctt tgcgctgcgc tgtcgccgac cacggcccaa
     4381 tgaaggtagg tcttgttgtc cggccgccac cagacgttga ggtcaaccac ggcgccggca
     4441 tgatctttca tgtgcggcgg caggagcccg cgaatggtcg agggcggcac cgacatcaag
     4501 agcaccggca gcagccgcag atcgacgtcg tactggcgcg caagctggac gaggcgcagg
     4561 ttcgggtcag gttgccgcat ggcttgcatg cgcgcctgcg acacgccggc ggcgaggctc
     4621 acgaagacct tgccttcact gcgttcgagg ccaaccatca tgcgggattg cgtttcaacg
     4681 cggttcgccg gccagcccag acgaaaggca cgttcgccgt cgctcaggga atagccctga
     4741 gagctggcga tgaacagcgc cacgtcgttg gtcggattcg atactccgag tttggccccg
     4801 ctcgacagga acgcgctggc cttttcgatc tccaccgacg agaaagcgac gcgccagtag
     4861 ttgccatagg tcagggaaac gtcgtccaga gcctgggcgg gacccacgct cgccagcacc
     4921 agtcctgcca aggcaaccgc cattgcccgg tggcgcatcg gcttcaactc caaagcacgc
     4981 cgggcaagca aaggtactgg tttgccgcag ttccagcatc ctgtggttgc aacgttgcga
     5041 attcacgccc cgacgccatc gttgtgcacg catttccgct cgccggcgct gcagtcggtc
     5101 gtgtaggagc gcagcgatga accagatcct ctccgttcag gtcctgcgcg ccctggccgc
     5161 gctgtccgtg gcctatggcc atgcggccta tgacgcgtcg cagattgccg cgcggtccgg
     5221 gcaaacgttc gagccggctt tccgggagct gagcgccgcc ggcgtggacc ttttcttcgt
     5281 gatttccgga tttgtgatgg tctggtcctc gcgttcgctg tttggccgac ctggcgcggc
     5341 ggggttgttc atcgggcgca ggctggcgcg catcgtgccc ttgtactggg cgaccacgac
     5401 ggtgttcctg ctggcgctcg ccatgcttcc gcaagcggtg aatgcagccg cgcccgccgt
     5461 gggcgacatc gtgaaatcct atgtcttcat tccgtttcgc cgccccggcg atggcagcct
     5521 gcagcccgtc tacgggttgg gctggacact gaactacgag atgtttttct acgtctgttt
     5581 cgccgggctg ctggcgctgc gggccggctg ggcgatcggc gtgttggtgg ccgtcttctc
     5641 gggcatcgtg gcggcagggt attggctgac gccgcagtcg gcgcccctgc agttctggag
     5701 ccagccgatc attctcgaat tcgtgctggg cgcgctggtc ggctatgcct acctgtctgg
     5761 cgtgcgtgtt ccaagacctg ttgggctggc tgcggtggcg ctggccgcct gctttttcct
     5821 ggcggcaacg ggcggaacgt ttccgcaggg tgcgtttgat cggctatgga tgcttggagc
     5881 gccggcggcg ctggcgctgg ccggcgcaac gttggtcaat caaagccccc gggaaggcgc
     5941 ggtctggcga ggactggcga tgctcggcga tgcttcctat gccctttatc tgatccaccc
     6001 gatgctcatc cgggccctga acatggtctg gaccaagctt ggcctggctc cgcaggtcgg
     6061 ctggcccttc atcatcgtgg ccctcctgct gagccttgcc agcgcggtgg tcgtctaccg
     6121 atggttcgag cggccgctga cgctctggct gcagggacga tcacggtcgt gacagctctt
     6181 ccggggcggg aggccaaggg cacgggggca cccgcatttt cgttggccgg acgcactgga
     6241 aaacggagcg caaggcggta tatcgtagcg gcatccagcc gttacttgcg tgtgccattg
     6301 caacagccga agacgatttc cgaagttctg acggcgttgc tggcagaagg cccgacctcc
     6361 gaggacggga agatgtcgct tgacgagatg gtcggcgcac tgaaacaccg ggctttcggt
     6421 atcctgtatc tgctcttcgg tttgccaaac tgcctgccga tgccgcccgg catcccaatt
     6481 ctgtgcggtg tcgtggtggc cgtgatctcg cttcagatgc tgctgggaca accggttctg
     6541 gtcctgccgc gctggctcgg caaacgacgt gtcagccgcg ccatgctgac cggcgtcgtc
     6601 gagaaatccg cgaaatacct cacggcgctg gagcgcttct cgcgcccgcg gtttccactt
     6661 ctgtcggggg acctgatgcg tcgcgccatt ggcctggcag gccttgcgct ggcggtcgcg
     6721 ctgatggcgc cgattccgct tttcggcggc attccagcgg gtatcgccgt cacgatcctc
     6781 gggctggcct ttacgcagcg cgacggcgtg ctgatcggct tcggcgtgct gatcgcgacg
     6841 cccgtcgcgc tggcggtgac cagcgccatg ctgttcgcgc tgttccaggg cgccgcagcg
     6901 atgttctgaa acccggcgtg gacggcgtca cgccgctgtt cgctggccgt tgatgtcgcg
     6961 gatgatcagg tcgatctcgt ccgccgtcgt cggcttggcg aagtagtagc cctggagatg
     7021 gtggcagccg accgcctgga ggaagcgccg ctgctcttcc gtctccacgc cctccgccgt
     7081 gacctggagc ccgagggccc ggccaagatg tatgatggaa tggagcagca ccgccgattc
     7141 cccggctgct tccatgcttt cgaggaatga ccggtcgatc ttgatccggt caaaggcgaa
     7201 gcggcgcaag tagatcaggc tggagtagcc gacgccgaaa tcgtccagcg caaggcggac
     7261 gcccatggtg cgcagctcca tgatggcggc ttcggcggcg tcggcatcct cgatcaccac
     7321 gccttccgtc agttcaagtt cgagccgtgc cgcatcgaac ccggtttccc ggatgatttt
     7381 cgaaaccgtt tcaacaaatg tcttctgctt gaactgaacc ggtgaaacgt tgaccgcaat
     7441 ggtcaggtcc ggccagcgct tgccgtcctc gcaggcgcgg cgcagcaccc attcgcccag
     7501 cggacagatc aggccacgtt cttcggcgat gccgatgaac tggttgggag cgatgcggcc
     7561 acgaaccggg tggttccagc gcacgaggcc ttcaagcccg acgatctttc cgccctcggg
     7621 cgtcacgatg ggctggtagt tcagctccag ctccccgcgg tcgatggcgt tgcgcaggtc
     7681 atcctcgacg agcttcttga tgcgcaactg ttcattcatc tcctgctcaa agaaggagaa
     7741 tcggttcttg ccctcgttct tggcacgata gagcgcgacg tcggcatagc gcatgagcgc
     7801 gggggcatcg ctgccgttgt cgggggcgac cgagattccg atcgacacgc cgacctgcgc
     7861 gtctgcgtcg tcaattctga aggggcgctg cagttcgccc agaatgcggc gcgccagcac
     7921 ctcggtatcg ctgatcgagc gcacgccgac ctgaatgatg gcgaattcgt cgccaccgaa
     7981 gcgggcgacc atgtccgccc cgcgaacgag atcgccaagc cggcgggcca ccatgccaat
     8041 gagtttgtcg cccgcgtcat gcccgagctt gtcgttgact tccttgaact tgtcgaggtc
     8101 cagatacatc acggccagtc cgccaccgtc gcgctggagt cgacgcagct cgtgatcgag
     8161 ccgtttcgag aagagcagtc gattgggcag tccggacagg ggatcgtggc cagccaggtg
     8221 ctgggccttg gcttcgttat gctccagttc gcgcgtgacg cgacgaatat gcatgtagac
     8281 cagcagaccg tagatgccga tcacggcaat gccgaggaag ccgatgctgg caaggctgtc
     8341 gagcgtgatc gagccgtcgt tgatgcggtt ccaggcgatc atggcaaagc cggtcccatc
     8401 aggagccgac tgtgcttcgg ggtggcggaa gcgcaagccg tcaagcatca gcgcgttgcc
     8461 gatttcggca agcacgggct cacccagaga cttggaggcc accagctcgt aattgccggc
     8521 gacattcacc gcagccagcg ccttgatggt gcttggctcg atgacgatgc ggctcatgcc
     8581 cagacggcgc agcgcatatt cagtcttctg gtcgcggcgg accgcaatga tctcgccggc
     8641 atccttcatt ctgcgttcga gaatgcccgg gctgcggaca tgggccgcaa ccagctgtcc
     8701 ggtcttctcg aaaaccgcag cgaactcgat gtcgtgggca tcggccagat agtcgatggc
     8761 cttctggtac tcgacgacgg tcttcggggt gctgtcctcg cgaaacagca gttgcaactc
     8821 cgcttcgatg ctgttggcaa agtcacgcgc cttttcttcg agcgccagtt cgatgcggcg
     8881 ctgctcaaga gccacggcgc gctgttcgtt ggtctcgaat gccgagcgcg ccaggaacga
     8941 catgctgccg atgacggcga aagtcagcac cagaatgggc acgatcgaga gcgatacgga
     9001 ctgtgttcgg tgtccgacat caggcgcgag cgacatgaat tttaccgatg gtctgaaatc
     9061 aagtgccgca atattgcaga tggaagttta ccattccttt gcaatcagag atggcgctac
     9121 ttatctgtgg ttgcaattct tggtggcggt cgcgggtgct ccgcccctcc agctacagca
     9181 atccgaggcc cttgaagctg gcgtggccgt cccgcccgat gatgatgtga tcgtggatgg
     9241 tcagccccaa gggcttggcg atggcgacaa tgtcctgcgt catcttgatg tcggcggacg
     9301 agggcgtcgg atcgcccgaa gggtggttgt ggacgatgat cagtgcggtc gcggacaatt
     9361 cgagcgccct acgcacgact tctcgcggat agaccggcgt gtggtccacg gttccggtct
     9421 gctgcacttc gtcggcgatc agcccgttgc gcttgtcgag gaacagcagg cgcagttctt
     9481 cacgctcgct gaaggccatc gagacacggc aatagtcgat cactgcgcgc caggaggaca
     9541 gcgattcgcg cttgcgcacg gcccctttcg ccatgcgccg cgatgccgct tcgatgatct
     9601 tcaggtcgat cgcggttgcc tcacccattc cgccgacttc gcgcaggcgt tcggtcgggg
     9661 cggcgacgac ctcggcgaaa gagccgaacc gtgcgatcag atccttggca agcgccttga
     9721 cgtccctgcg ggggatcgag cggaacagga cgagttcgag caactcgtag tccgcgagcc
     9781 catctccacc cgtagtcgtg aaccgctggc gcaggcggtc acggtggccg tggtagtgcg
     9841 gcgtctcgtc tgtcatcggc cgtagggcgg ctggtgcagt cccttcgggg acagcgtgaa
     9901 aatctcaacc ccgtcctggg tcacgccaac ggtgtgttcg aactgggccg acagggaccg
     9961 gtcccgggtc accgccgtcc agccatcgga cagaactttg acatgcggct tgccgaggtt
    10021 gatcatgggt tcgatcgtga agagcatgcc gggcttgagc tggacgcctt caccgggctt
    10081 gccgaaatgc aggatgttgg gctcgtcgtg gaagagctgc ccgagcccgt ggccgcagaa
    10141 gtcgcgtaca acggaacagc gttcggcttc ggcataggtc tgaatggcat ggccgatgtc
    10201 gccggtcgtg ccgccggggc gcacggcggc gatgccgcgc atcagcgcct cgtgggtcac
    10261 ttccacaagt cgttgtgctt tcctgggcac gtcgccgacg agatacatgc gacttgaatc
    10321 gccatgccag ccatcgacga tcaacgtcac gtcgatgttg acgatgtcgc cgtcccgcag
    10381 gggcttgtcg tccggaatgc cgtggcacac aacgtgattg atcgaggtgc agcacgcgcg
    10441 ggtatagccg cggtagttga gcgtcgccgg cagggcgtca tggtcttggg cgaaatcgaa
    10501 gaccagccga tcgaggaaat ccgtcgtcac gcccggccgg acatggtcga tgagcatgtc
    10561 gagcgcctcc gccgtgagcc gtccggcctt gcgcatggcg gcgaacgatt ccggtccgtg
    10621 tatccggatt tgtcccggac ggcgcttgat gggggcggct tcgagattgt tcatgattcc
    10681 ggagctgagc aggtgctttg catcctatgt aggcattcag gccgtgcacg ccaatctgta
    10741 agttcggcct ctgcgtttgg ggtttgcggc cagagcggct tgatctatga agcgcgcgat
    10801 acggaaagac cccgatgcca gacaaggaac caccctttgg cgcgtatgcg ccgacgggac
    10861 tcatctcccg gttgctggga gccacgcgcg cgctaccgcc gcagggcgtg agcaagcgca
    10921 ttggcttcgt gctgcgccgg atcgccattc tgctgctggg cggccggccc ctcgacgtcg
    10981 tttcgttcgg cgtcaggttc cggctgcacc cctactacaa cgtctgcgag aagcgcatcc
    11041 tgttctctcc gcgacagttc gacgagatcg agcgggacct gatcgcgcag cgaatccggc
    11101 cgggtttctg cttcgtcgat gtaggcgcca atatcggcgg ctatgcgctg ttcgtggcgg
    11161 cccaggcggg gcagggcgcc cgcatcctgg ccatcgagcc ccaaccggag atcttccggc
    11221 gactgatggc gaacatcggg ttcaatccct tcggcaccgt caaggcgatg gattgcgcgg
    11281 tcgcggacag cgaaggcgag gtcacgctgt tcatcgacgc cgagaaccag ggcgaatcca
    11341 gcctcaagat catcaccgcc ggttcccgat cgagcgtgcg cgtgccggca aggcggttgc
    11401 tgtcgatcct tcgggacgag ggtttcgacc ggctggatgc catcaagctc gatgtggaag
    11461 gggcggaaga cctgatcctc gagacattct tcgccaaggc gccggaaaag ctctatcccg
    11521 gcctgatcgt gatcgaacgg gcgccagacc gctggcaagt cgatctgccg agtctgcttg
    11581 tggccaaggg ctaccggttg attgctgaga cgcggaacaa cttcgtgttc gagcgcgggt
    11641 ttgccgcgga ctgaaccggt tcagcccagc acgaagggca cggcacgcgc caaccccaga
    11701 ccgtctggag aaacgtcgca gctgtaggca agggcttcga cgccagccgc ccgggcggcg
    11761 tcgaaggcgc gggcgtaggc gggatcgatg tcggcggcaa ggctgaaact atcggcgcgc
    11821 atctggatga catagagcat caccgcgcgt ccgccgtcgc ggaccacgtt ggcgagttcc
    11881 cccagatgct ttgcgccgcg cgcggtgacg caatctggaa actcggcctg gccggcgcgc
    11941 cggcacaggt gcacgttctt gacctcgaca taacagggag cgcgctccgg tgcttccaaa
    12001 agcagatcaa tgcgcgaatt gacgccgtat cgcacttcgc ggcggagcgt tggatagccg
    12061 gcaagttcgg gcagggttcc cgccgagatg gccaattccg ccagccggtt ggggtggctg
    12121 gtgtttatgc cgacacattg cgtaccgtcg ccgaagtcga cctcgaccag ttcccagttg
    12181 taggcgagct ttcgcttcgg gtccggcgac atcgacagcc agaccgtgct cccgggatcc
    12241 gtgagaccca gcatcgcgcc tgggttggcg cagtgcgcgg tgacgatgct tccgtcagcc
    12301 agcgcgacct ccgcgaggaa gcgcttgtag cgctggatca ggcgtccagg gacgatacgc
    12361 gaggggaagc gcaatcgcgg catgattccg ggcggtgtgg tgcactgtcg atgtgctttg
    12421 cagttgaccg tacctatccg gtcctgttca gttccgccat gccctacact ctgaccttcc
    12481 accgcgtatt ccgtgcgtct cccgaaatcc tctaccgggc tttcactgat gcgcaggcca
    12541 aggtgaagtg gctgccgccc aacgggttta ccgccaaggt gcatcacctc gatctgcgtg
    12601 tcgggggcgg ttacgccatg agcttcacca gtatcgcgac gggtgagtcg cattcgtttc
    12661 gtggtgtcta cgacgagtgc gaaccgggca agcgtctgcg ctatactgac cgttttgacg
    12721 atcccaacct gccgggcgca atgcatgtca ccgttgactt ccgtgcggtt tcggtcggaa
    12781 ccgatgtgtc gatccgtcag gacggcgtgc cggacgtgat cccgccggaa gcctgtcatg
    12841 tcggctggca gcagtccctc gccttgctgg ccctgcttgt ggaggcgaat gtctgaacgg
    12901 cggcgaaggg cgcgacgccc ttcaccgtcc attccaaccc ggttcgagtg cgatgactcc
    12961 acatcccgac gttactgccg cccttctcgt catcggcgac gaaatcctgt ccggccgcac
    13021 caaggacaag aacatcggct acatcgccga gtaccttacc ggcgtcggca tcgatgtgcg
    13081 cgaggtgcgc gtcgtgccgg acatcgaggc ggaaatcgtc gctgccatcc tcgcattgaa
    13141 ggagcgctac acctacctgt tcaccaccgg cggcatcggg ccgacgcatg acgacatcac
    13201 ggcggattgc gtcgccaagg ccatgggcgt ttccattgac cacgatccgc gtgccgtggc
    13261 gatcctgcga gagcggtatc gtccggaaga cctgaccgag gcgcggctgc gcatggcgcg
    13321 cataccggcc ggggcggagc tcatcgagaa cccgatctca aaggcgccgg ggttcatgat
    13381 tgccaacgtc attgtcatgg ccggcgtgcc ggcgatcatg caggccatgc tcgataacgt
    13441 cgcgggacgg ctgaagaccg gcgcgcgcat gcagtccgtc accatcgact cgggcggact
    13501 gcccgaaggc atttatgccg aggcgctagc atcggtcgcg agagaccatc caggtgtcgt
    13561 cattggttcg tatccgtcgt ttacgcacaa gggcttcgag aacaagatcg tcctgcgttg
    13621 caaggacacg agtctgctcg cccatgccga aaaagctgtg atcgacgccc tggcgcagct
    13681 tcaagccgcc aaggcttcgc gttagagcga aacgagacct acggagaacc agcgatgaat
    13741 gcacagccgt tgcccgagaa ggcctttccc gtctcgtggg accagttcca tcgcgacgcc
    13801 cgggcgctcg cctggcggct ggcggccgcc ggacccttcc gcggcatcct gtgcatcacg
    13861 cgcggcggtc tggtgccggc ggcgatcgtg gcgcgtgaac tggacgtcag gctgatcgat
    13921 acggtctgcg ttgcatccta ccacgattac gccaaccagg gcgaactgaa ggtgatcaag
    13981 ccgatcgaca cggggctgat cggcgaggat ggcggcaagg gcgtgctcgt ggtcgatgac
    14041 ctggtcgaca ccggcaagac catgaaactg gtgcgcgaca ccatgccgaa cgcgcattac
    14101 gcggcggtct acgccaagcc gttggggcga tcgatggtcg agactttcgt caccgaggtc
    14161 tcgcaggaca cctggatcta tttcccctgg gacatgggct ttgccttcca gccgccgatc
    14221 accaagggtt cggccgggta ggagccatcc tggtttcgtt ttctcatgac atttgagggc
    14281 gcgtgtcact tgccaagtgc gcgcacgccc tgaaagatgc cgttgatgtc cgccgtaaga
    14341 atgcgggctt cgggaggcat ccttggctga agtgtttgat gcgtcgcggt tccgcaagac
    14401 cctgcccgac gcactgctcg atgcgcggga tcggcatggc ggcgcgaaag aggcgctgga
    14461 agatcccgag cgcacgccgc tcagttatga ccggctgatc ctcggcgcac tggtgcttgg
    14521 ccgcaagctg gccggcgagg ctgcggcggg cgcgcgagtg ggagtgcttc tgccgaatgt
    14581 gaatggcgcc gtcgtgacgt tcttcggcct gctgtttcac gggctgacgc cggtcatgct
    14641 gaacttcacc gccggactga agaacctgac ttcggcctgc gagacggcgc agatcgggat
    14701 gatcgtcacc tcgaagcgct tcgtcgacac agccaagctc gatgatgtgg tcgcgggcct
    14761 cggtcaggga cggcgtgtcc tgtatctgga ggacgtacgc aagcagatcg gggtgctcga
    14821 caagatcgct ggccttgtgc ggtcgcgatt tgcgcgcgcc atcgcccggc gtgcctgtcc
    14881 cgaccccgac gcgccggcga cgatcctgtt cacctcaggc tcggaaggcg tgcccaaggg
    14941 cgtggtgctg acccatgcga acatcgccgc caatgccgga cagatctgga agctggcgga
    15001 cggcatgctg agcgaagccg acaccgccat gaacccgctg ccgatcttcc actccttcgg
    15061 gttgaccgca ggactgctgc ttggcctgtt caacggcatg aaggtggtgc tgtatccctc
    15121 gccactgcac tacaagcagg tgccaaagct cgctggcgag accagggcga cgctgctgtt
    15181 tgcaaccgac acgttcctcg ttggctatgc cagggctgcc gacccgaacg acctcgtcag
    15241 cgtgcgactg gtcgtcaccg gggcggaaaa ggtcaaggac gggacgcgca agctctggga
    15301 accctacggc gcaaccatcc tcgaaggcta tggctgcacc gagtgttcgc cggttctcgc
    15361 ctgcaacacg ccgacggcga accggccggg aacggtgggc aagctgctgc cgggcatcga
    15421 ggcgcggctt gatccggtcg agggcatccc ggagggtggg cggctcgtgg tgaaaggcgc
    15481 caacgtgatg gcaggctaca tgctggccgc gcggcccggc ttcgtgcagc ccccagacgg
    15541 cgggtggtac gataccggcg acatcgtcga tattgatgcg gagggcttcg tcaccatcaa
    15601 ggggcgggcg aagcggttcg ccaagcttgg cggggagatg gtctcgctcg cggcggtgga
    15661 ggccatggcc tcgacccttt gggaaggcgc cacgcatgtg gcggtcacgc tgcccgatgc
    15721 caggaagggg gagcaggtgc ttctcgtgac cgaccggccg gacgccgacc gggcagcgct
    15781 catcgaattc gcgcgggagc aggggatgcc ggaactgttt gtgccccgtg ccgtcctggt
    15841 ggtgggggcc gtgcctgtcc tgggctccgg caaggtggac tatgccggag cggctgagct
    15901 tgccaggtcc atgcgaccgc tgatctgaga agcgcgcgac gtgccggcaa agcgctggcg
    15961 caaaaatact taggacaata agattgcgag tcgaaagcag tcgatcgtgt cttcatcctc
    16021 cttcccgccc gtgacgccct ccgaagcccg ccgccagcgg ctgctcgcga tcgccatgac
    16081 ctgtggcgct gtgctgtgtt tcacgctgat cgacacggca gccaaggcgc tttccccgca
    16141 ggtcggcgtg atgaccacga cctggctgcg ctatgtcgcg gccttcctgt tcatcctgcc
    16201 gtttctcaac ccctggcggg ttccagacct gctgaagagc gagaagccgt tcctgcaatc
    16261 gctgcgcggc ctgttgctgt tcggatccac gatcctgaat tttgtggcga tgcagtacct
    16321 ccagctggcg caaaccgtca gcatcggttt tctggcaccg cttctgttcg ccctgcttgc
    16381 cggacctttc ctcggcgagt gggcagggcc acgccgcctc atcgccatcg tcatcggctt
    16441 catgggcgtc ctcatcgtcg ccaggcccgg cctcggcgtc attcatcccg ccgcgcttct
    16501 cagcatctgc agcgccacct gctattccat ggtgctgctg ctgacgcgca atctctccgt
    16561 ccgcgacagc gcggagacga cgatgttcta ttccacgttc gtcggtgtcg tgttgatggc
    16621 tccgctggcg tttctcaact ggaagactcc ggagttcccc tcgggctggg cgctggttgc
    16681 gctgacggga cttggcggcg cgctcgggca ctggctgatg atccaggccc agcgtcgagc
    16741 cgccgcgcct gtgctggcgc ccttcatcta tttccagatc gtctggatga gcctttccgg
    16801 ttacgtcttc tatggcgaac agcctgaccg ctggaccttt ctgggcggtg caatcgttgt
    16861 cgcctccggc ctttatctgc tgcatcggga gcggaagcgg aagccggccg cgggagcggc
    16921 ttcctgaccc cggctcgcac cggcacccca cccggctgtt tctggacttc cttatcccga
    16981 tccatgggat tgccgcgcca gcactgcgga ttgcgcaggc atttcctgcc cggtctgcat
    17041 gcgtggacct ggcacaggcc attgtgccgg gtcaatcgaa ggcctatggg ttgtgcaacg
    17101 acaaagtcgg tctggggagt agcgcatggc agaggacggc gagcgccggc aaggggcgat
    17161 caagtcaccg caggatttgg ccgcgagctt gttcctgttg ggaatagcgg ttctggcttt
    17221 gtggctgggt cgggatcttt ccaccggaac gctgcgccag atgggccccg gcatgctgcc
    17281 gaaggccttg gccgtgctgc ttgcgggcct cgccgttgtt ctcctgatcg atgccttcaa
    17341 ggaggcgggc gaagagcttg ccgcctggtc tccgcgggcc atcatctttg tcctcggtgc
    17401 cttctgcgcc ttcggcttcg cggtgcgccc gctcggcctc tccgtcgccg ggccgttggc
    17461 ggtgatcatc gcgggatttg cctcgaacga gacgaaattc gttgaaacgc tgatcttcgg
    17521 gctggtgatg acggcgttct gcgtcggctt gttcaagttc gccctcggac tgcccattcc
    17581 gcttgcaccg tggctgatcg gatattgagg gttccgacat ggacatcatc cagaacctgg
    17641 gcctcggctt ttccgtcgcc ctgacattcc agaacgccgc gctgtgcttc atcggctgcc
    17701 ttgtcggcac gatgatcggc gtgcttccgg gcgtcggccc aatcgccacc attgccatgc
    17761 tgttgccgct gaccttcaag gtcgacccga ccggcgcgct gatcatgctg gccggcatct
    17821 actatggcgc gcagtacggc ggctcgacca cggcgatcct gatcaacatt cccggcgagg
    17881 caacgtccgt cgtgaccgcg ctggacggcc atatgatggc caagaatggc cgtgccggcg
    17941 ttgcgcttgg catggccgcc atcggctcct tcatcgccgg caccattgcg acactggtga
    18001 tcgcactgct gggcaagccg ctgacagcca tggcgctgat cttcggaccg gcggagtatt
    18061 tttcactgat ggttctgggc ctggcgctgg cggtggcgct ggcgcgcggc tcggttctga
    18121 aggcgatcct gatgatcgtg ctcggcatcc tgctctcgac agtcggcacc gatctcgaga
    18181 ctggcgagga acgcctgacc ctcggacttc aggacctctc cgacggcatc gactttgccg
    18241 tgctggcgat gggtgtcttc ggcctcgctg aaatcctgaa gaacctcgaa aatccggagg
    18301 cccgcgatgt ggtgaagagc aagatcggcc gcctgctgcc atccatggct gagttccggc
    18361 agtccctggc tcccatcctg cgcgggacct ctcttggctc gatcctcggc atcctgcccg
    18421 gcaacggcgc ggtcctggct cccttcgcca gctacaccat cgaaaagaag atcgccaaag
    18481 acccttcgcg tttcgggaag ggcgccatcg aaggcgtggc tggaccggaa gccgccaaca
    18541 acgccggcgc ccagacggcc ttcatcccgc tgctgaccct cggaattccg ccgaatgcgg
    18601 tgatggcgct catggtcggc gccatgacca tccacggcat cattcccggt ccgctggtga
    18661 tgacgaagtc gccggatctg ttctggggca tgatcgcctc gatgtggatc ggcaacctca
    18721 tgctggttat catcaacctg cccttgatcg gcatctgggt gaagctgctg caagtgccct
    18781 acaggctgat gtatccggcg atcctgctgt tctgcgccat cggcatctac tccataaaca
    18841 acaacccgtc tgacgtgttc ttcaccgcct tcttcgggct cgtgggctat gtgttctaca
    18901 agttcggact ggagccggcc ccaatgttgc tcggctacgt gctcggtaag ctcatggagc
    18961 agaagttgcg gcaggcgctg acgctgtcac gcggcgattt catgacgttc atagaacggc
    19021 cggtttccgg tgtgctgctg ttcatcgcgc ttgtgatcat cgtgctggcg ctgttgccat
    19081 ccatgcgcaa ggaacgagag caggtattcg ccggtgactg agtgatctcg ctcaaggcga
    19141 tgccacaagc acatccctat gtgtcacctg cctcgacaag cttccacgga accctaattc
    19201 caacccagag tgaaaccagg gagagaccct caatgaaacg ccttcttctt gccgccgccg
    19261 cggctgtcgg cctcgccgct gttccggcgc aggcgcagga ctatccgaac aagccggtaa
    19321 cgatgatcgt accgtttgca gccggcggtc cgacggacat cattgcgcgc cttgttgcgg
    19381 agagcatgtc caggacgctc ggtcagcagg tcatcatcga gaacgtggcg ggcgctggcg
    19441 gcaccaccgg ctcgacccgc gcctcgcagg ccgcagccga cggctacacc atcatgatgg
    19501 gccacatggg cacgcatggc gcggctcccg gcctctacaa gaacctgaag tacaatccgt
    19561 cgaccgattt ttcgcccatc ggtctggcgg cggggacgcc catcatcatc gttgccaaga
    19621 aggcctttcc ggcggacgat ctgaagggct tcatggccca tgtgaaggcc aacaacgaca
    19681 agatcacgca ggcgcatgcc ggcgtcggtt ccgtttcctt caccacttgc atgctgctga
    19741 cggctcaact cggcgtgaag gcgaccgctg tcgcctatac cggcaccggc cccgctctga
    19801 acgatctcgt cggcggtcag gtcgattaca tgtgcgatca gatcccctcg gtcgtggccc
    19861 agattcgcgc cggatcggtc aaggccatcg cgatcgccct tgatgcccgt tcgccggccc
    19921 tgccgaacct cccgacgacc aaagaagccg gcttgccgga gttccaggtt tcggcctgga
    19981 acgccgtgtt cgcgccgaag aacctgcctg cgccgattca ggccaagctc gccgacgcgc
    20041 tgtccaaggc gctggacgac gacaaggtga aagcgcgact cctcgatctc ggcgccgtta
    20101 tcccgcaggg cgatgggcgc ggccccgacg cgctcgccaa gctcgtgaag agcgaagtcg
    20161 agcgctgggg cccgatcctg aaggcggtgg cgcaataagc gcctttccgg aaaagcaaaa
    20221 agggcgccct tgggcgccct tttcatgcgc acctgtgcgc aagtcgctta gcggcagacc
    20281 ttcaaacgct cggtcttcac gtagttgccc tgctcgtcga acagccggac cggacgcacg
    20341 gaacagccgc cggctgcctg gtagctgacc gggcgcgctg tcgaaccggt atattccggc
    20401 cgagcagggg cgctcggctg ctgggaccga tggtaaagcg tcgcgccgac tgcgccgaca
    20461 gcgacacctg cggccgcgcc tatgagcgcg gacttcctgt tgccatcttt ggcctgcgcc
    20521 tcgccactga cgcccatcag gccagcggca acgatggcga ccggaaccag aatcttgaac
    20581 ttcatgatcg ttactcgact gatcgcgccg ccgccgggct gcgccgcgcg cgtgaacacc
    20641 acctgggccg cgcttgccgc gctggccgat cccgtaccga cattccgctc gaagtgttgc
    20701 gaatggctta aggttatcga aacaggcctg atcgcagccc ttgcctactt caagaatgta
    20761 aatatattaa acataaacat caaggagagg gcatggcgag gcgcattctt gtcattctgg
    20821 ggcatcccgc agagcagagc ttttgccgtg ctctggcgga aagctatgtg gccggcgcgg
    20881 gaagcgctgg ccactatgcg cgagtgatcg acctccgggc cctgactttc gatccggtcc
    20941 tgcatggcgg ctatggtggg gaacaatcgc tggaatctga tcttgtgcgc gcgcaggaaa
    21001 gcatcctgtg ggcccagcat ctggtcttcg tctacccgat ctggtggggt gccgtgccgg
    21061 cactcctcaa gggctttatc gatcgggtgt tcctgcccgg ctttgccttc aagtatcaca
    21121 aggacaagat ttcgacctgg gacaaactgc ttagcggccg cactgcccga ctgctggtga
    21181 cgatggattc gccgccctgg tacttccgtt tcgtcagtcg cgcgcccggc cacaaccaga
    21241 tgcgtcgcac cattcttgag ttctgcgggg tcaagccggt ggcgatttcg tcgtttggtt
    21301 cggtgcaagc gtccgatgcc gcaaggcgcg cggcgtggct gaagcaggcc gaaggactag
    21361 gctccgccgg gagttgacgc cgtcgcaaac cgggttcggc atttccgccg cttgcaccag
    21421 cgcatggaaa tccccatctg cttgcggaac ttctcgatcc gcaacggatg atccatgaca
    21481 gcctccctag aactcggcct cgacactttc ggcgacgtta ccgccgatga agcaggccgg
    21541 ccggtatcac acgcgcaagt catccgcgac cttgtcgatg aggcggtact ggccgatcag
    21601 gtcggcctcg atttcatcgg tgtcggcgaa catcaccgcg atgacttcgc cgtctccgcg
    21661 ccagaggccg tgctcgccgc catggcggtg aagacgcgcc gtattcatct gggatccgcc
    21721 gtcacggtgc tgtcctccga cgatccaatc cgcgtgttcc agcgcttcgc gacggtcgat
    21781 gcgctttcga acggccgtgc ggaagtgatc ctcgggcgtg gcagcttcac cgaatccttc
    21841 ccgttgttcg gcttcaacct gcgcgattac gaggttctat tcgaggacaa gctcgacctg
    21901 ttcgcgcggc tggcgagcaa cgaggcagtg acctggcaaa gcccggcctc gccgccgacc
    21961 cgcaccccgc tgaagaacca acatgtctat cccaagctgg agcatggccc gctgaaggtc
    22021 tggatcggag tcggcggcag cccggaatcg gtggtgcgcg ccgcgcgcta cggattgccg
    22081 ctcatgcttg cgatcattgg cggggagcca gcgcggttca aaccttttgt tgatctctac
    22141 gggcgtgcct gcgaacagct gggccagccg cggctgccta tcggcgtgca ttcgccgggc
    22201 tatgtcgcgg acacggacgc ggccgcgcgc gaagagttct tccaggatta caaggcgatg
    22261 cgcgaccgca tcggccgcga gcggggctgg ccgcccatgg gcagaggcga attcgaccat
    22321 gaggtcgaga aaggctcgct ctatctcggt tcgcccgaaa cggtagcgaa aaagattgcg
    22381 aaaacggcgc gggcgctcgg catttcgcgg ttcgacctga agtactccgc cggcccattg
    22441 ccgcatgcgc ggctgatgcg ctgtatcgag ctctacggaa ccaaggtcat gcctatggtg
    22501 cgagagatgc tagcctgagc ttggtgcgga cggcgggggt cgaacccgca cggagttgcc
    22561 tccgagagat tttaagtctc ttgcgtctac cggtttcgcc acgtccgcaa aggcccttcc
    22621 gccggccggg gccgggaggg tggaccgctc aaagcctata gcgtgccggg ataggccccg
    22681 ccatcggcaa cgaggttctg cccggtgatg aaaccggatt tcgccgaaca caggaaggcg
    22741 caggcatgac cgaactcctc ggcggtgccg aagcggttcg ccgggatgcg cgcctgcatt
    22801 tccgaaacgg tggcgtcccg gtccttgccg gacttcttca tgttgttgcc gatgattgcg
    22861 gtcaggcgat cggtgtcgtg ctgccctggc agcaggttgt tgatggtgac gttgtgcttc
    22921 gacacagtgc gggccagcgt tgccacagcg ccggtcaggc cggcgcgcac gccgttcgac
    22981 agttccagca tcgcgatcgg cgctttcacc gaggccgaag tgatgttcac gatgcgcccg
    23041 aaaccgcgct ccatcatgcc gtagaccgtg gcctggatca gcgcgatcgg cgtgaaaaag
    23101 ttgccctccg cggccttgcg ccagtcatcg agggtgaaat tgcggaaatc gcctgccggc
    23161 ggcccggccg cgttggtgac aagaatgtcc gggtcggggc aggccgcgag caccgccttg
    23221 cgtccttctt ccgtcgtcac gtcgcaagca acaggcgtga ccttgacgcc gaacttcgcg
    23281 gcgatctctg cggcggcgct ctcgatgtcc gcccgggtgc gggcgcagat gacgagatca
    23341 accccttcgg cggccagtgc ttcggcgcaa ccgcgcccca gccccttcga ggcagcacag
    23401 acaatggcct tgcggccctt caatcccaaa tccattcctg acctcgatgg cagatagtgc
    23461 gggcgcgggt ccgggcccga cgcaatcgcg ttgttcaacg gtcctgagta tgccgtcgcg
    23521 gccttgtgac gcaaccatga aaaaggcggg actgagcccg ccctcagcga agaccgcaag
    23581 cgcgttcagt ccacgaattc gttgtcctcg cgatggccaa cccgcatcgc catgttcgaa
    23641 tcggtttcgc ggacctcaac cttgcagcac cagatgcgat cctgctcgcc atactgcttc
    23701 aggaagatgg tgttgatgta ttcgtagaga aagtcagcca aaccctcgca cccggtcttc
    23761 tcgacctcgg taatctgcgc aatgccaagc ttgcccaggt tgagcagttc ttcgcgatgc
    23821 ggatcatcgg cggcaaccag cagcttgtgg tcgaaccact cgtcaagcaa ctccttgagc
    23881 ggcttgagcc cgccgaaatc catgcaccaa ttacgggcgt cgaggtcctt gcaaccgaac
    23941 tcgaaatgga aagacagtgc gtagccgtgc acgaacttgc agtggctgtc ggctcgccac
    24001 tggcgatagg cgaccgggcc gatctgtcta taggtcttgg tcgagatgta cttgtagcgc
    24061 ggttcgactg cagttctgac gacgggaata ttcatggctg tccctcccac atcgcttcta
    24121 tagcgcagtt tccgtaaagg aagcagttaa ttggcgccgc ccagatcctg ttttccttaa
    24181 tgcgcgtccc gttcaggcgc gatggtcgta tatccatcca taacgcctgg cgagcacgtc
    24241 gtgcccgagc agcgccatgg ccgcatcgcg cacgatcgcg gccgggttgg caaggtggta
    24301 gatggacgcc tgccggtgtg aaaggcgttg aatgcgcgct gcccggcgca ggcgttcctc
    24361 ctcgtagcct ttcagggcgt ccgaaatcga ggcatggccc cgcagcgatc gcgaaagcac
    24421 ggcagcgtcc tcgatcgcct gggccgctcc ctgtgccagg aagggcagca ttggatgggc
    24481 tgcatcgccc aacagcaccg ccggagatcg cgaccaacgc cattccggtg cccggtcgaa
    24541 cagagaccag cgcagccagg tgtcggcgga accgatcagt gcagtaaggt cggaatgcca
    24601 atcctcaaat cggcgattga tccaggcgat gtctcctggc tccgaccagc cgcgctcgcg
    24661 ccagttttct tcgacaacag cgacaacgtt gataacgctg ccgccgcgca ggggataatg
    24721 aacgacgtga gcgccagacc cgagccacag attggaacgc atctcgcggg cgaatgccgg
    24781 gacagagtcc gccgggatga gcgtgcgcca tgcgacgcag ccggaaaacg aggccggtcc
    24841 ggtcagcccc acctgaccgc gcaccttgga ccacaaccca tcggcgccaa tcaggagatc
    24901 ggtctcgacc gtcaactcgt cagtatagcg ggacagtgtt gcggtgacgc ttctgtctcg
    24961 gaacttcagt gcttcaagcc gccgaccgag atgcaacgcg atgctggatt gctctgtggc
    25021 ggcggcgagc agggcgttct ggagatcgcc gcgatgaacg acaaggaagg gggctcccca
    25081 gcgcttctcg gcggtctcgc ccagcggcat gctcgtcaaa gtcgacccgt tgcgcccgga
    25141 acggacgacc atcgcctccg gctcgaccgc aaggccatcc agcctgggca gcacaccgag
    25201 atccgccagc acgcgcgagg cgtttggcga aatctgcagt ccggaaccta ccggctccag
    25261 cctgtgcgcc tgctcgaaga cctgcacctg ccagccatcc cgggcaagcg acaatgcggc
    25321 ggtcaagccg ccgattcccg ctccaacgat aactgccgat ttgcccccgc tcacggcatc
    25381 aggccctcag gccgcttcgt gccagg
//
LOCUS       DAOMXN010000015        23242 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_1362_length_23242_cov_12.255014, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000015 DAOMXN010000000
VERSION     DAOMXN010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 23242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23242
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1362_length_23242_cov_12.255014"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="PK812_02855"
     CDS             complement(<1..648)
                     /locus_tag="PK812_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HRJ68521.1"
                     /translation="MRRRRRTVQALGLAAGLVVAALVAAKAEQIGTAGAVNPAAQSAP
                     PGGAMRTMNVGGNIVFKERINTSDSGSVQLVFVDRTTLNIGPNSQLTIDEFVYDPKAN
                     TGRMTATLTKGVMRFVGGQASHTGGATIKTPATTLGVRGGVVTVRHDQQNGTRAINHF
                     GVMTAQSGQGTEVIRRPGFALNVANNAAPPSAPARAPQSEIDANNRQLTSAPQQTG"
     gene            complement(659..1915)
                     /locus_tag="PK812_02860"
     CDS             complement(659..1915)
                     /locus_tag="PK812_02860"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ68522.1"
                     /translation="MGNSELKREHDALFAEVLKNPSNVDVSFRYAEVATRLGDYEAAI
                     GALERIIFYNPNLPRVRLELGVLYFRLGSYEMSKSYFESAIAPPDTPIEVRNRVAGFL
                     AEIDRRVQTTHLAFFGQVGLRFQTNANAGPNTPFVRALGFDATLSNQNTRKRDWNWYG
                     SASLRYVYDFENQRGDVFEALVSTYYSRQFDVVRLNTGLVEVNLGPRLALAPDLLQGW
                     SVKPYVVGSAVSLADNNYLNTAGYGVTFAMPLIVVLLEPGYEVRRRAFKNSDEITTAK
                     EQRGWIHTSYLNASGALLPGLRFQARYAHNRVNAFMDYNAYTSNAVDVSFPFEFAGLF
                     ESAGKWTIAPFGGFTKSLYDRPNPLVDPNYRRKDKEFRYGVQIDMPIWKNAGFGAVVQ
                     YQNMSSSIRNYNTRNFSVSFGPTVRF"
     gene            complement(2273..3103)
                     /locus_tag="PK812_02865"
     CDS             complement(2273..3103)
                     /locus_tag="PK812_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009492523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRJ68523.1"
                     /translation="MFGLPLGDLALFAAALLAAGVITGLLAGLFGVGGGAVIVPVLYQ
                     IFGMVGVPESHRMHLCVGTSLAIIIPTSISSFRTHMAKGKVLMDVLKAWALPVLAGVV
                     LGSFVAAYAPGLVLKCVFIVVALTNATKLLAGRDDWRLAPSLPGRRGLGVFGFVIGLA
                     SSLMGIGGGVISNMFLTLHGVSMHAAVATSAGLGVLISIPGAIGYVLAGWPEVADLPP
                     LSLGFVSILGFLLVAPTSTFLAPYGARLAHNTPKRRLEIAFGLFLLLTAGRFVLSLLD
                     "
     gene            3237..3632
                     /locus_tag="PK812_02870"
     CDS             3237..3632
                     /locus_tag="PK812_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HRJ68524.1"
                     /translation="MTVQPQTNDPDFTSRDYWNGLIKMGLSKLFILAAINEQPRHGYD
                     LARRVQEMTRGCCSPTAGALYPALTAFETGGYVTVRSEFHGGRERKVYELTDKGREAL
                     AVAIQSWRDASAVLAVPHCPPCAAPEGRV"
     gene            3646..4857
                     /locus_tag="PK812_02875"
     CDS             3646..4857
                     /locus_tag="PK812_02875"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRJ68525.1"
                     /translation="MTPRIAWAIAGLGVTQIVSWGSTYYLLTVMGPPIAADLGLPPEF
                     VAFGQSLMLITSGLLGPLAGRIMDTRGARPVMTSGSLVSAGGLGLLALARDPFLYLVA
                     CLVVGISGAMVLYSAAFTALTQLDPARARRNITLLTLPGGLASTVFWPLGTWLLTHVG
                     WREVCLIYAMINVLLCLPIHVLVAAARGKDGRLDAAPATADVEGLPKSARQRAFLLLA
                     TMLALQSLATVGLFNQILTFLTGLGHSQDLAIRIAMLFGASQVSARVFEMLFGGRYEV
                     SITGVVAAFGLLAAMLVLPLSTQVPAAGYVFAALLGASNGLFTIVQGALTVALFGSRG
                     YGARIGSISVARAFTSALGPVLFAAVLARIGAWGLAVFCIVVSTGVLVVMLMLFQHIR
                     RHAQDRTNPGA"
     gene            4859..6247
                     /locus_tag="PK812_02880"
     CDS             4859..6247
                     /locus_tag="PK812_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-efflux pump"
                     /protein_id="HRJ68526.1"
                     /translation="MSAPANGLAQKKQHAALISVIASAFIALFKLIAGLASGSLALLS
                     EAGHALIDLGATVLTLFAVRTSEKPADDVHHYGHGKIEAVAALVETGLLFVLAGFVVF
                     EGVKRLLGGGETIAVTWPVIAVLVASILIDLNRWWHLSRVAEETKSVALAADALHFSS
                     DLISSTLVLVGLGLVAAGFPQADSLAALAVAAFILVAGWRLGRRTVDTLIDAAPEGVR
                     DQIRAALESTSGIISVESVRVRPAGATLFVEATVTVPRLLPLDRVQAIKEQASAAIRA
                     AVPEAEPTVTAEPRAMDDESILERVILIAAKRRTPVHHVTVQTVGGQLSVSLDVEVDG
                     RMSLAAAHQIASKLETAIRSDLGPETEVETHIEPLEAQGLVGADVPENEKAAIEEQIR
                     ALALADPLIGEVHSLRARRTDKGLVVSLHVRADPQASVSDVHLAVDRLEHKARSANAE
                     IARIVTHAEPRR"
     gene            6328..8946
                     /gene="pepN"
                     /locus_tag="PK812_02885"
     CDS             6328..8946
                     /gene="pepN"
                     /locus_tag="PK812_02885"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HRJ68527.1"
                     /translation="MRTDIAPVIRLSDYRAPDFLVETVDLDFNLQAEATRVRAVMRLR
                     RNPEGFDTTPLVLEGDDLSLAEVRLDGRLLGHDDYTVAPDKLVLANPPSGDFTLTVET
                     IVNPAANTKLMGLYRSGGNYCTQCEAEGFRRITYFLDRPDVMAVYTVRIEADRDEAPI
                     LLSNGNPGESGTIPGTNRHYAVWRDPFPKPSYLFALVAGDLGVLSDSFLTRSGRAVAL
                     NIYVEKGKEARAAYAMDALKRSMRWDETAFGCEYDLDVFNIVAVSDFNMGAMENKGLN
                     VFNDRYILASPDTATDQDYHNIEAIVAHEYFHNWTGNRITCRDWFQLCLKEGLTVFRD
                     QEFSSDMRSRPVERISAVRTLRASQFVEDAGPLAHPVRPSQYKEINNFYTATVYNKGA
                     EVIRMLKTVIGPEAFRRGMDLYFRRHDGQAATIEQFVACFASAAERNLEKFMLWYQQA
                     GTPVVTVTQAYDAAAQTLRIDFEQQVPPTPGQPEKLPMVIPILMGAIGENGADVISDR
                     VLVLETDKGSKTFQNVAERPVLSLLRGFSAPVRLKFERDDNDLLLLARHDRDPFSRWE
                     ALQTYATRLLKRSVAEIRAGRQPLTDAGLAQAMAEIVREGMVDPAFAELLLTLPGESD
                     MAREIGADVDPEAIAQAVDAVADHIGRACLVDAQALYNRLAEPKPFSADAASAGERAL
                     KAAVLALVARGDRDAGALLALDQYHQAATMTDRMSALRVLNRIGGAEREEAFAAFEAA
                     FAHDPLILDNWFSLNALVRTDDALVRVRNLTDHKAFSIANPNRVRALVGAFAMSNPRQ
                     FNRADGEGYRFVSEFIRDLDAKNPQVAARLATAFRTFGTLEPGRREKARQALEMLQAQ
                     PSLSRDLKDIVDRALA"
     gene            9269..11587
                     /locus_tag="PK812_02890"
     CDS             9269..11587
                     /locus_tag="PK812_02890"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRJ68528.1"
                     /translation="MARTQAANARSRIATSSGLATTVAEPGYHRLISAEIWAQRIVPV
                     LIAVFLAVVGTSAFFQMTGSRDESLADHKNDLDMIATLVSSRLREVLQPSAERSSEEA
                     FRLLLPTATFSYGRRVYIVDGHGAILHGSSAGQRPAAYIDDVIQGSQPLLSFGDRAGV
                     LLVKETNRGEALATARRIPATQQGASHVIVVQSVSDALTNWRGRTKAFVTLFLTLGGV
                     VAALGAAFYVQAARARQADRICNRMTVRIDSALAEARCGLWEWDLSLGRFFWSDSMYQ
                     LIGLARGSDLISLAEIQALSHPDDSSLFALADRMLASNGQTAEYEFRMRHSAGHWVWL
                     RARLQLIRDSEASHPRLIGVVIDVTEQKQFEEMTRRADVRLSDAINSISEAFVLWDHD
                     NRLVLCNAKFRQLHALAPETVVPGARYEDVIPHTSLEMETGSTGERGDRSLSRVYEAQ
                     MPDGRWLQVSERRTMDGGFVSVGTDITTHKDQEARLMDSERRLLATVADLRKSRQALQ
                     IKTVELAELADRYKEKTIAAEAASNAKTAFLANMSHELRTPLNAIIGFSEMMEHKIYG
                     DLGSRHYEEYAGLIRRSGSNLLTIINDILEMARIEAGNVAIDVSELPVTPLVQSVADL
                     VAEEAAAKRIDLKLDLRADLWVRADARAVRQALAHLLHNAIKFTPDGGRAAIRVRRAM
                     TGVNIFIEDTGVGISPENLARFGRPFDVMEGKLINGCKGSGLGVAIARSLVELHGGGI
                     RVRSWVNSGTIVLVHLPIAPPPGTRDPDSLAA"
     gene            complement(11594..13006)
                     /locus_tag="PK812_02895"
     CDS             complement(11594..13006)
                     /locus_tag="PK812_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRJ68529.1"
                     /translation="MTGFGKLLRTTAFRLAVVYLVIFVLFAGAVLGYVAWNTRRLLES
                     QLSETIETEINSLAEQYRIGGMRRLAATIERRSRQARGFLYLLADTRSEVLAGNATEL
                     VQVLQSPPGWREVPFRRADEQESFITLAKVRIFVLPNGMRLLVGHDLEERQRLRQVIR
                     QAMGLSILLVFVLGGFGALMVSRRVLRPVEDIAEAGRRIMSGDLKERLPVRGVGDEFD
                     RLAESLNQMLARIDELMSGMRAVSDNIAHDLRTPLTRLRAGAEEALRNARSPDHLRQA
                     LERTIEESDALIKVFNALLMIARAEAGSTPGAMTDVDLAKIVRDVGELYDPVAEEARL
                     RLVVEAQTPVVVRGNRELLGQALSNLVDNAVKYGVPVEGADAPAEVRLTLGCEGGTAL
                     LVVADHGVGVPPAERERVLDRFVRLEKSRAQPGFGLGLSLVAAIARLHGGNFRIEDNA
                     PGVRAVLTLPRASAVPVAAQ"
     gene            complement(12990..13676)
                     /locus_tag="PK812_02900"
     CDS             complement(12990..13676)
                     /locus_tag="PK812_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRJ68530.1"
                     /translation="MTMKILIVEDDAEAAAYLAKGLREAGHVADHAGDGEKGLEMALS
                     APYDVMIVDRMLPRRDGLSLVSAVRGEGRETPILVLSALGQVDDRVVGLRAGGDDYLS
                     KPYAFTELLARVEALGRRRGSSPAQTVYRVGDLEMDRLSHRVARGATEIDLQPREFRL
                     LEYLMRNAGQVVTRTMLLEHVWDYHFDPQTNVIDVHMSRLRSKIDKGFDKPLLHTVRG
                     AGYMIRDGLR"
     gene            complement(13842..14336)
                     /locus_tag="PK812_02905"
     CDS             complement(13842..14336)
                     /locus_tag="PK812_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HRJ68531.1"
                     /translation="MMRRQARFLAAAMMIAVLSGPAFGLSPAERLPNPAQEQRAREIG
                     AELRCLVCQNQSIDDSDAELAKDLRRLVRERISAGDSDRQVKDFLVARYGEFVLLRPV
                     FSMKNLLLWTLPFLALIAGIFGVRRLFGRQVAAPVAQAALSAEEQAELDRIVAAQDSI
                     TKNS"
     gene            complement(14333..16303)
                     /locus_tag="PK812_02910"
     CDS             complement(14333..16303)
                     /locus_tag="PK812_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016553312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HRJ68532.1"
                     /translation="MIAELGHFALVLALACSAIGTVLPLWGAVREDRALMATARPAAL
                     AQFVLLALAFAALTAVYVQSDFSVLNVYENSHTDQPLIYRFSGVWGNHEGSILLWVLI
                     LALFGACVALFGRHMPEALRAATIAVQSSIAFAFLLFIALTSNPFLRVFPAPAEGRSL
                     NPILQDPGLAIHPPLLYLGYVGYSIVFSFAAGALILGRIDAAWARVVRPWNLIAWTTL
                     TAGIAMGSYWAYYELGWGGFWFWDPVENASLMPWLTGTALLHSAIVMERREALKVWTI
                     LLAVLTFSLSLLGTFLVRSGVLTSVHAFASDPARGVFILLILCLFIGGAFALFAWRGP
                     ALAGGALFSPVSREGSLVLNNLFLSAACVAVLTGTLYPLALEAVTGDKISVGPPFFNA
                     TFLPLCAPLVLAMPFGQQLAWKRGDLLPVAQRLFVAILVGAGMFVAVLAITRTSEAVA
                     AFGLGVGMFVTVGALDDLRVRASRGAAGTWMRRFVGLPKSVHATAIAHAGVGLMTIGI
                     AATAFHEEKIATMAPGDKAIIGGYELVFEGLRKQPGPNFASTVGRFVVSKDGVALAVL
                     EPGKRTYVGRTMQTSETGRLTRGLGQIYLAIGDAHGASVDVRAYWKPWVLLIWLGPLA
                     MTIAGLISLMDRRLRVAAPSRRKLPAMPEAAE"
     gene            complement(16300..16767)
                     /gene="ccmE"
                     /locus_tag="PK812_02915"
     CDS             complement(16300..16767)
                     /gene="ccmE"
                     /locus_tag="PK812_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015930694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HRJ68533.1"
                     /translation="MTRKQRRITLIASAGAILALAVALILTALKDRIVFFRSPTEVQQ
                     MAIKPGQRFRLGGLVENASVERAVGQRISFRVTDGNASITVRHVGILPDLFREGQGVV
                     CEGVLHADGSFLADTVLAKHDENYMPREVSDALKKQGHWQGEAKPGEAGGKPK"
     gene            complement(16764..17855)
                     /gene="ccmI"
                     /locus_tag="PK812_02920"
     CDS             complement(16764..17855)
                     /gene="ccmI"
                     /locus_tag="PK812_02920"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HRJ68534.1"
                     /translation="MLFWFLAAGMVAAVVLVLLMAITPKPLASGGGDFDVAFYKSREA
                     EIARLEAEGLMAADDAKAALAEAARRLIGVKRGGSAAIGNDASRRRLAQALTVLAIPA
                     LTLSLYLTQGRPELPGMPYASRTDLNRSDEELTRLIGRLDAHLADSPDDARGWELAFP
                     VYMRLGRFENAVAAAERLIALKGDTPERQASLAEALIFNARGVVGDAAKAAIDKALAG
                     DPKLARARFYRGLAAEQSGDLDGARAVWQALHDDLPDGNEKTAIRSNLARLGPTGAAA
                     DAIRSLPEAERRTAIRGMVDALAIRLKQDGGSAEDWQKLIRALAVLGERDKLIAAVAE
                     ARAAFRGKDGAAAIEALAGELGLPAEAKP"
     gene            complement(17938..19317)
                     /locus_tag="PK812_02925"
     CDS             complement(17938..19317)
                     /locus_tag="PK812_02925"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRJ68535.1"
                     /translation="MRIRSLSGRLLLSATLLCCTVLLIAGLVLSELNRRSVTQAFDER
                     LRLYLKALVADVATLTDGTSTDVGNLGEPQFQIPLSGWYWQIVKLDQEKDTAAVMRTS
                     RSLFSTKLPFLIDPIDPRRENDASDGYITGPESKRLRILEQLIDLGDNNRFYISVAGN
                     AAEIQDETNRFNTALTITFVLLGAALIGSTVVQVGYGLMPLAELRRAVFAVRQGDAEH
                     VGERFPQEVAPLAHELNELIDANREIVNRARMQVGNLAHALKTPISVLVNEAEQDTSA
                     LALKLREQTGIMKDQVQYYLDRARAATRAVTIGSVTEVDPVIDAFLRTFPKIYRDRDV
                     AYVSTGAKGLRFRGERQDLEDLIGNLIDNAGKWARSRVEVSIAPVPVDPGAAPRFSVL
                     IDDDGPGLTPENRMQATRRGRRLDETKPGSGLGLSIVVDLAALYHGTFELDDSPLGGL
                     RAKLVLPAV"
     gene            complement(19324..20001)
                     /locus_tag="PK812_02930"
     CDS             complement(19324..20001)
                     /locus_tag="PK812_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRJ68536.1"
                     /translation="MRLLVVEDDKDLNRQLVTALQNAGYAVDTAFDGEEGHFLGDTEP
                     YDAIVLDLGLPRMDGVSVLEKWRKGGRRMPVLILTARDRWSDKVQGFDAGADDYVTKP
                     FHMEEVLARLRALVRRAAGHATSELSCGPVLLDTRSSRVIVDGNPVKLTSHEYRLLAY
                     LMHHQGRVVSRGELVEHLYDQDFDRDSNTIEVFIGRLRKKLGVDIIETVRGLGYLVGQ
                     PGKAKSG"
     gene            complement(20128..20859)
                     /locus_tag="PK812_02935"
     CDS             complement(20128..20859)
                     /locus_tag="PK812_02935"
                     /inference="COORDINATES: protein motif:HMM:NF017638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 108 family protein"
                     /protein_id="HRJ68537.1"
                     /translation="MAKGNFERCLAHTLAHEGGFADHPADPGGATNLGITRAVLAAWR
                     GRPVSKAEVRGLTRAEAAAIYRQRYWDAIGGDHLAPGVDLATFDYAVNSGPKRALRAL
                     ATARSDTPQATIRAVCAERRGFLGKLRIFAVFGRGWMARVAAVERAALAMATGQPSPS
                     IASNTSNTTTRKTQAMTPTKSMFESRTVWANLVGLTAVAASMLGFNTGMIDQNAVVEA
                     ILNAVAGASFVASTMYRVAATRRID"
     gene            complement(20867..21250)
                     /locus_tag="PK812_02940"
     CDS             complement(20867..21250)
                     /locus_tag="PK812_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68538.1"
                     /translation="MKVLSARYANLEGTAAIIATDSAGAVALSAPDHPEAFAALLESG
                     VAIAPYEAPPAPVRCASALALRYALNEIGQRAAWDTALAAMSQGTRDYWVSEPNPPET
                     SPKLNRIAAAAGVDLKALFDRARAG"
     gene            complement(21247..22929)
                     /locus_tag="PK812_02945"
     CDS             complement(21247..22929)
                     /locus_tag="PK812_02945"
                     /inference="COORDINATES: protein motif:HMM:NF022436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2793 domain-containing protein"
                     /protein_id="HRJ68539.1"
                     /translation="MSETTPLLGLQLTAAGQAQKHVTVNEALLRLDAIVQLAVIERNR
                     DTPPAAPANGDRYIVGAAPTGAFVGHAGAVASFDAGGWNFLAPGVGWCAYVIAENALL
                     AFDGSGWSATSSTGGITELQNLVKLGLGTMADGANPLAVKVNNALFAAESTPSGTGDL
                     RFKLNKDAAANTVSQLYQTGYSGRAETGLMGDEQYAIKVSADGATWKQALVIDPTSGV
                     VDFPFGATGAGGGGGGAASGINALVNVGLSASVAGGALTVDLKTAALAAPSGSDTVMI
                     PFRSATASSGSVATREVATPTSLTLSSGSTLGVAAINTPFALWIVAFDDGGTVRLGII
                     NCLSGENIFPLGRGSPIASSTAEGGAGGADSAHVFYTGAAVASKPYVVLGRLEWSSGL
                     ATLGTWTAPTNVAVYSAGMKLPGDLVQIASDRYTANAALSTSIPNDDTVPTSTEGTQI
                     VSATLTPTSAANRIHARFAGPFDSTSAIYAYAALFNGGSAAIGTVMSTINGNGYPTVL
                     AMERAQAASSTSSQTWSVRVGGSSAIYANGLSSGRKLGGSMGVTLIVSEVQA"
     gene            complement(22958..>23242)
                     /locus_tag="PK812_02950"
     CDS             complement(22958..>23242)
                     /locus_tag="PK812_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68540.1"
                     /translation="VTFDWIRRTRRGGDNWEALDVPLGEESEAYRLDIRSMAGALLRS
                     IAATQPSCAYAAADELADFGAPQAALNVRLVQISRAVGDGMPFAGIVPIR"
BASE COUNT         4234 a         7566 c         7474 g         3968 t
ORIGIN      
        1 gccagtctgc tgcggtgcgc tggtcaactg acggttgttg gcgtcgatct ccgactgcgg
       61 cgcgcgggca ggcgcactcg gcggcgccgc gttgttggcg acattgagcg caaagccggg
      121 gcggcggatc acctccgttc cctgcccgga ctgcgcggtc atgacgccga aatggttgat
      181 ggcgcgcgtg ccgttctgct gatcgtgccg caccgtcacc acgccgccgc gcacaccaag
      241 cgtcgtcgcg ggcgtcttga tcgtcgcgcc gccggtatgg cttgcctgtc cgccgacgaa
      301 ccgcatcacg cccttggtca gagtcgcggt catgcggccg gtattggcct tgggatcata
      361 gacgaactcg tcgatggtga gctgcgaatt cggcccgata ttcagcgttg tgcggtcgac
      421 gaacacaagt tgcacggacc cggaatccga ggtgttgatc cgctccttga aaacgatgtt
      481 gccaccgaca ttcatggtcc gcatggcacc gccgggcggg gcgctctgag ccgccggatt
      541 gacagcgccg gccgtgccga tctgctcagc cttcgccgcg accagagccg cgacaaccag
      601 tccggctgcc agtcccaggg cttgtactgt ccgtcgtctt ctgcgcatgg ccacctcctc
      661 agaaccgaac cgtcgggccg aagctcacgg aaaagttccg agtattgtag ttgcggatgc
      721 tggaactcat gttctggtac tgaaccaccg cgccaaaacc agcgtttttc cagatcggca
      781 tgtcaatctg gacgccatag cgaaattcct tgtccttgcg ccggtagttc ggatcgacaa
      841 ggggattggg gcggtcgtac agcgacttgg tgaagccgcc gaacggcgcg atcgtccatt
      901 tgcctgcgct ctcgaacagc ccggcgaatt cgaacggaaa cgagacgtcg acggcattgg
      961 acgtgtaggc gttgtagtcc atgaaggcgt tgacgcgatt gtgcgcatag cgcgcctgga
     1021 accgcagacc aggcaggagc gcgcccgacg cgttgaggta gctggtatga atccaaccgc
     1081 gctgctcctt cgcggtggtg atctcgtccg agttcttgaa ggcacgccgg cgcacctcgt
     1141 accccggctc aagcagcacg acgatcagag gcatggcgaa cgtcacgcca tagccggccg
     1201 tgttcaggta gttgttgtct gcaaggctga ctgcgctgcc aacgacatag ggctttacgg
     1261 accagccctg gagcaggtcc ggcgccagcg ccagtcgcgg tcccagattg acctcgacaa
     1321 gcccggtatt caggcgcacg acatcgaact gacgcgaata ataggtcgaa acaagcgctt
     1381 caaagacgtc gccgcgctgg ttctcgaaat catagacgta gcgtagtgat gcggaaccgt
     1441 accagttcca gtcccgcttg cgtgtgttct gattggagag cgtggcgtcg aagccgaggg
     1501 cccgcacgaa gggcgtattg ggtcccgcat tggcattcgt ctggaacctc aggccgacct
     1561 gaccgaagaa cgcgaggtgc gtggtctgca cgcgccggtc aatctccgcc aggaagcccg
     1621 caacacggtt gcgcacctca atcggcgtgt ccggcggcgc gatggccgat tcgaaatagc
     1681 tcttcgacat ctcgtacgag ccgagacgga agtagagaac gcccagttcc aggcgcacgc
     1741 gcggcaggtt gggattgtaa aaaatgattc gctcgagcgc gccgatcgct gcctcgtagt
     1801 cgcccaggcg cgtcgcaacc tcagcatagc ggaacgagac atccacattg gacgggttct
     1861 tcaggacctc ggcgaacagc gcatcatgtt cgcgcttcag ctcagagttg cccacggagg
     1921 ataaggactg cgccgctgcc gggggcgggg cgaccatgca aaggccaagc gcgaccgcca
     1981 cgcccaatgc gcgtgcactc ctgagaattc tctcgagggc gtgataaccg gacattcgcc
     2041 tccatcacct tcttgacgcc cgttcgcgcc tttcaggcta ccgcctctga attcctgcga
     2101 aaagccgctc aataaaagtt tggcgatact cgaaaatcaa tgccgttgcg gcgcagcaat
     2161 agacgggcgc ggctgactcg tgtcaacggg gatcggccaa tccgaaacag gcaccatcag
     2221 cctttccatt tccttgtggt gtttctatca cacggaacag aagcagcgaa gactagtcga
     2281 gcaggctcaa gacaaatcgt ccggccgtca gcaataggaa caggccaaaa gcgatctcaa
     2341 gccgacgttt cggcgtattg tgggccaggc gtgccccata gggagcaaga aaagtcgacg
     2401 tcggcgcaac aagcaggaag ccgaggattg aaacgaagcc gagagacagc ggcggcaagt
     2461 cagcaacttc cggccagccg gcaagaacat agcctatggc tccgggaatc gatatgagca
     2521 cgccaagccc ggcggaggtc gccaccgctg catgcatgga aacgccatgc agcgtcagga
     2581 acatgttcga aatgacaccg ccgccgatcc ccatcagcga ggaagccaga ccgataacga
     2641 acccgaagac gccgagccca cgcctgcccg gcagagatgg cgccaggcgc cagtcgtcgc
     2701 ggccggcaag cagcttcgtg gcgttcgtca gcgccacgac gatgaagacg catttcagca
     2761 ccaggcccgg cgcgtaagcc gccacgaaac tgccaagcac cacgccggcc agcaccggca
     2821 aagcccaggc cttcaacacg tccatcagca cctttccctt cgccatgtgg gtacggaaag
     2881 aggagatcga cgtcgggatg atgatggcaa gcgacgtacc gacgcacagg tgcatgcgat
     2941 ggctctcggg tacgccgacc atgccgaaaa tctggtagag cacgggcacg atcacggctc
     3001 cgccgccgac gccgaagagc ccggccagca ggccggttat gacaccggcc gccagcaacg
     3061 cggcagcaaa aagggcaaga tcccccagcg gcagtccgaa cacgtacatt tccccaggct
     3121 gttgcggtcg atccggacgc tgcgccggca tgcccggaag gtggccttcc tttgcccttg
     3181 tggcgccata gatcgaagtc agatatatcc cgcctcgata cacgcgtcga tggatcatga
     3241 cggttcagcc acagacgaac gatccggact tcaccagccg ggactactgg aacggactca
     3301 tcaagatggg tctgtcgaag ctgttcatcc tggcggccat caacgagcag ccgcgccatg
     3361 gctacgatct cgcccgccgg gttcaggaaa tgacgcgcgg ctgctgttcg ccgacagccg
     3421 gcgcgcttta tcccgccctg actgcattcg agacgggcgg ttacgtgacg gttcgcagcg
     3481 aatttcacgg cggacgcgag cgcaaggtct acgaactgac cgacaaggga cgggaggctc
     3541 tcgccgtggc gattcagtcc tggcgcgatg ccagcgccgt actcgccgtc ccgcattgcc
     3601 caccctgtgc cgccccggag ggcagggttt gagggtccga agatcatgac gcctcgcatc
     3661 gcctgggcca tcgcgggact tggcgtcacg cagatcgtca gctggggatc aacctactat
     3721 ctgctgacgg tcatggggcc accgatcgcc gccgatctcg gcttgccgcc ggagttcgtc
     3781 gcgttcggcc agtcgctgat gctgatcacc agcggcctgc tcgggcctct ggccggccgg
     3841 atcatggata cgcgcggcgc gcgccctgtc atgacatcag gcagcctcgt ttctgcaggt
     3901 gggctcggcc tgctggcgct ggcgcgcgac cctttcctct acctggtcgc ctgcctggtg
     3961 gtcggaattt ccggagccat ggtgctctac tcggccgcgt tcacggcgct gacccaactc
     4021 gatccggcgc gagcgcgtcg gaacatcacc ttgctgaccc tgccgggcgg ccttgcttcc
     4081 accgttttct ggccgcttgg cacctggctc ctcacccatg tgggctggcg cgaggtttgc
     4141 ctcatctatg ccatgatcaa cgttttgctg tgtctgccga tccatgtgct tgtcgccgcg
     4201 gcgcggggca aggatgggcg gctcgatgct gcgccggcca cggcagacgt cgagggattg
     4261 cccaagtcgg cccggcaacg tgcgttcctg cttctggcga ccatgctggc gctgcaatcc
     4321 ctcgcgacgg ttgggctgtt caatcagatc ctgaccttcc tcaccggact tggccacagc
     4381 caggatctgg ccatacggat cgccatgctg ttcggcgcct cgcaagtgtc cgcgcgcgta
     4441 ttcgagatgc tgttcggcgg ccgctacgag gtttcgatca ccggcgtagt cgccgccttt
     4501 ggccttctgg cggcgatgct ggtcctgccg ctctcgacac aggtcccggc cgcaggctat
     4561 gtcttcgctg cgcttctggg cgccagcaac gggctcttca cgatcgtgca gggggcgctg
     4621 acagtggcgc ttttcggatc gcgcggctac ggcgcgcgca tcggatcaat ttcggtggcg
     4681 cgcgcgttca cgtccgcact cggtccggtg ctgttcgccg cagttctggc gcggatcggc
     4741 gcctgggggc tggccgtttt ctgtatcgtg gtgtccaccg gcgtgctcgt cgtaatgctg
     4801 atgctgttcc agcacatacg ccggcacgct caggatcgca ccaatccggg agcctgaaat
     4861 gtctgcacca gcgaacggct tggcccaaaa gaagcagcac gccgcactga tctcggtgat
     4921 cgccagcgcc ttcatcgcgc tgttcaagct gatcgcaggg ctagcgtcgg gatctctggc
     4981 gctgctgtcg gaggcaggtc atgccctgat cgatctgggc gcaacagttc tgaccctgtt
     5041 tgcggtgcgc acctccgaga agcccgccga cgacgtccac cactatggcc acggcaagat
     5101 cgaggcggtg gcggctctgg ttgagaccgg gcttctgttc gtgctggccg gctttgttgt
     5161 cttcgaaggc gtcaaacgtc tccttggcgg cggcgagacg attgccgtga cctggccggt
     5221 tatcgcggtc ctggtcgcct cgatcctgat cgacctcaat cgctggtggc acctgagcag
     5281 ggtggcggag gagaccaaga gcgtggcgct ggcggcggac gcactgcatt tttcctccga
     5341 cctgatcagt tcgaccctgg ttctcgtcgg tctcgggctg gtggctgccg gttttccgca
     5401 ggccgattcg ctggcagccc tggcggtcgc ggcgtttatc ctggttgccg ggtggcggct
     5461 cggcagacgc accgtcgata cgctgatcga tgccgcgccg gaaggggttc gcgaccagat
     5521 acgagccgcg ctggaaagca catcggggat catcagtgtc gagagcgtcc gggtgcgccc
     5581 ggcgggcgcg actttgttcg tcgaagccac cgtcacggtt ccgcgcctcc tgccgcttga
     5641 tcgcgtgcag gcgatcaagg agcaggccag cgccgccatt cgcgccgcgg ttccggaggc
     5701 cgaaccgacg gttacggcag agccgcgcgc catggatgac gaaagtatcc tggaacgcgt
     5761 cattctgatc gccgccaagc ggcggacacc cgtgcaccac gtcacggtgc agacagtcgg
     5821 cgggcagctg tcggtatcgc tggacgtgga agttgacggg cgcatgagcc tggcggcagc
     5881 gcatcagatc gcctccaagc tcgaaaccgc aatccggtct gatcttggcc ccgagaccga
     5941 ggtcgagacg catatcgaac cgcttgaggc ccaaggcctc gttggagctg acgttccgga
     6001 aaacgaaaag gcggccatcg aggagcagat ccgggcgctg gctctggccg atccgctgat
     6061 cggtgaggtg cattccctgc gtgcccgcag gaccgacaag gggctggtcg tcagcctgca
     6121 cgtcagggcc gatccgcagg ccagtgtgtc cgacgtgcat ctcgccgtcg accggcttga
     6181 gcataaggcg cgctctgcga atgccgagat cgcccgcatc gtcacccacg ccgaaccgag
     6241 gcgctagcca gtctcgccct cgcttgacat ccgcacgggc tggcgcaacc cctgtttcag
     6301 agcctccgac agacgacagg accttccatg cgcaccgaca tcgccccggt catccgcctt
     6361 tccgactacc gggccccgga tttccttgtg gaaacggtcg acctcgattt caacctgcag
     6421 gctgaggcga cccgcgttcg cgcggtgatg cggttgcgcc gcaatcccga aggtttcgac
     6481 acaactccgc tggttctcga aggcgacgac ttgtcgctgg ccgaggtccg gctcgatggc
     6541 aggctgcttg gccatgacga ctatacggtc gccccggaca agctcgtgct cgccaatccg
     6601 ccgtccggcg acttcacgtt gaccgtcgaa accatcgtca accccgcggc caacaccaag
     6661 ttgatgggcc tttaccgctc tggcggcaac tactgcacgc agtgtgaggc ggaagggttc
     6721 cggcgcatca cctatttcct ggaccggccg gatgtgatgg cggtctacac ggtacgcatc
     6781 gaggccgacc gcgacgaggc gccgatcctg ttgtccaacg gcaatccggg cgaaagcggc
     6841 accattccgg gtaccaaccg ccactacgcg gtctggcgtg atcccttccc caagccgtcc
     6901 tatctgttcg cgttggtggc cggcgatctc ggcgtcttgt cggatagctt cctgacacgg
     6961 tcggggcgtg cggtggcgct caacatctat gtcgagaaag gcaaggaggc gcgtgcagcc
     7021 tatgcgatgg acgcgctcaa gcgctcgatg cgctgggacg agaccgcctt cggctgcgag
     7081 tacgatctcg atgtgttcaa catcgtcgcc gtgtcggact tcaacatggg cgcgatggag
     7141 aacaagggcc tgaatgtctt caacgaccgt tacatcctcg ccagccccga cacggcgacg
     7201 gatcaggatt accacaacat cgaggcgatc gtcgcgcacg agtacttcca caactggacc
     7261 ggcaaccgca tcacctgccg cgactggttc cagctttgcc tcaaggaagg gctgacggtg
     7321 ttccgcgacc aggaattctc gtccgacatg cgttcgcggc cggtggagcg aatttccgcc
     7381 gtgcgcaccc tgcgcgcctc ccaattcgtg gaggatgccg gccccctcgc ccacccggtc
     7441 cggccaagcc agtacaagga gatcaacaac ttctacacgg cgacggtcta caacaagggt
     7501 gccgaggtca tccggatgct caagaccgtg atcggcccgg aggcgttccg ccgcgggatg
     7561 gacctctatt ttcgccggca cgacggccag gccgcgacca tcgagcaatt cgtcgcctgt
     7621 ttcgccagcg ccgccgaacg caacctcgaa aagttcatgc tgtggtacca gcaggcggga
     7681 acgccggtgg tgacggtgac gcaggcctac gatgccgcgg cacaaaccct gcgcatcgat
     7741 ttcgagcagc aggtgccgcc aacaccggga cagcccgaaa agctgcccat ggtcatcccg
     7801 atcctgatgg gagcgatcgg cgagaacggc gcggacgtca tttccgaccg cgtgctggtg
     7861 ctggagacgg ataagggctc gaagaccttc cagaatgtgg ccgaacggcc cgttctgtca
     7921 ttgttgcgcg gcttttcggc gcccgtccgc ctgaagttcg agcgcgacga caacgatctg
     7981 ctgctgctgg cgcggcatga tcgcgacccg tttagccggt gggaagcgtt acagacctat
     8041 gcgacgcgcc ttctgaagcg ctcggtcgct gaaatccgtg cgggccgtca gcccttgacc
     8101 gacgcggggc tggcgcaggc aatggccgag atcgtgcggg aaggcatggt ggaccctgcc
     8161 ttcgccgaac tgctgctcac cctgcccggt gaaagtgaca tggcgcgcga gatcggtgcc
     8221 gacgtcgacc ccgaggccat cgcgcaagcg gtggacgccg tggccgatca catcggacgt
     8281 gcctgcctgg ttgacgcgca ggccctttac aaccggctgg ctgagccaaa gcccttttcc
     8341 gccgatgcag ccagcgccgg cgagcgcgcc ctgaaggccg cggtccttgc gctcgtggcg
     8401 cgcggcgacc gagacgcagg cgcgcttctg gcgctcgatc aataccatca ggctgcgacg
     8461 atgaccgatc ggatgtcggc gttgcgtgtc ctcaaccgca tcggtggtgc agagcgggaa
     8521 gaggcgttcg ccgcttttga ggcggccttc gcccatgatc cgctgatcct cgacaactgg
     8581 ttctcgctga acgcgctggt gcggaccgac gatgccctgg ttcgggtgcg caacctgacc
     8641 gaccacaagg cgttctcgat cgcaaatcca aaccgggtgc gcgcattggt cggtgccttc
     8701 gccatgtcca atccgcgcca gttcaaccgg gcggatggcg aggggtaccg gttcgtttcc
     8761 gaattcatcc gcgatctcga tgccaagaat ccgcaagtcg ccgcccgcct tgccaccgcc
     8821 ttccgcacct tcggaacgct ggaaccagga cgccgcgaga aagcgcgcca ggcgctggag
     8881 atgctgcaag cgcagccctc gctttcgcgc gacctgaagg acatcgttga ccgggcgttg
     8941 gcgtgagccg gcagccggca gccggaccgg ccctgatcgg cttacctacc gcagggcgac
     9001 agtcgcagac gctgcctgta acagcagacc agaaaataga acgcattgga acaaaataga
     9061 acataaatag aacatgtcgc cgttcgccag cgggaatggt ttcggcaagg gcagctttcg
     9121 attccaaaaa gcaacaaaat gctctgacgg gattcgactg ccctttttgc tctggacagt
     9181 ccgattcgtt gcgattcaac tacagctgat tcggggcggc aaaagcggcg catccccggt
     9241 ttcagggcga agttgacggg ggccaagcat ggcgcgcact caagcggcca atgcacgatc
     9301 gcgtattgcc acaagttcgg ggctggcgac cacggtcgcg gaaccggggt accacaggct
     9361 gatcagtgcg gagatctggg cacagcgcat cgttccggtg ctcatagccg tatttctggc
     9421 cgtagtcggc accagcgcat tcttccagat gacgggatcg cgcgacgaat ctctggccga
     9481 ccacaagaac gatctcgaca tgatcgcgac gctggtgtcg tcgcggctgc gcgaagtcct
     9541 ccagcccagc gctgaacgca gctcagaaga agccttccgg ctgctgctgc caaccgctac
     9601 tttttcctac gggcggcggg tctacatcgt ggatggtcat ggcgcgatcc tgcacggcag
     9661 cagcgcgggc cagcggccgg cggcttatat cgatgacgtc atccagggat cgcagcccct
     9721 cctctccttc ggagacaggg ccggtgtcct gctggtaaag gaaacgaacc ggggcgaggc
     9781 actggccaca gcccgccgca ttcctgccac gcagcagggc gccagtcatg tcattgtcgt
     9841 ccagtccgtt tccgacgcgc tgaccaactg gcgaggccgc acgaaggcgt tcgtaaccct
     9901 gttcctgacg ctcggcggcg tcgttgccgc cctgggggca gctttttatg tccaggcggc
     9961 acgggcccgg caggcggatc gcatctgcaa tcggatgacg gttcgcatcg attccgctct
    10021 ggccgaggcg cgttgcggtc tttgggaatg ggacttgtcc cttggccgct ttttctggtc
    10081 cgattccatg taccagctga taggcctcgc tcgcggcagc gatctgatca gccttgcgga
    10141 gattcaggcg ctcagccatc cggacgacag cagcttgttt gcgctcgcgg accgcatgct
    10201 ggcgagcaac gggcagaccg cagagtacga gttccgcatg cgtcattcag ccggacactg
    10261 ggtctggctg cgcgcgcgcc tccagctcat ccgcgacagc gaagcttcgc atccgcgcct
    10321 gatcggcgtg gtgatcgacg tcaccgagca gaagcaattc gaggaaatga cccgccgtgc
    10381 ggacgtacgc ctgagcgatg caatcaactc gatctcggaa gccttcgtac tgtgggatca
    10441 cgacaaccgg ctcgtcctgt gcaacgcgaa gttccggcag ttgcacgcgc tcgcgccgga
    10501 gaccgtggtg ccgggcgccc gctacgagga tgtcattccg cacacatccc tggaaatgga
    10561 aacaggatcg accggcgagc gcggcgaccg ctcgctatcc cgcgtctatg aagcgcaaat
    10621 gccggacggg cgctggctgc aggtcagcga gcgccgcacg atggacggcg ggttcgtctc
    10681 cgtcggaacc gacatcacca cccacaagga tcaggaagcg cggctgatgg acagcgagcg
    10741 ccgcctgctc gccacggtgg cggacctgcg caagtcgcga caggcgctgc agatcaagac
    10801 ggtcgaactc gctgaactcg ccgatcgcta caaggaaaag accatcgcgg ccgaagccgc
    10861 cagcaatgcc aagactgctt tcctcgccaa catgagccat gagttgcgca cgccgctcaa
    10921 cgccatcatc ggcttctcgg aaatgatgga gcacaagatt tatggcgatc tcggctcgcg
    10981 gcactacgag gagtatgcgg gcctcatccg ccgttccggt tcgaacctgc tcaccatcat
    11041 caacgacatt ctggaaatgg cccggatcga ggccggcaat gtcgcgatcg atgtgtctga
    11101 actgccggtg acaccgctgg tgcaatccgt cgccgatctg gtcgccgagg aagcggctgc
    11161 caagcgcatc gacctcaagc tggacctgcg cgcggacctg tgggtccggg cagacgcgcg
    11221 ggccgtccgg caggcgctgg ctcatctcct tcacaacgcc atcaagttca cgccggatgg
    11281 cgggcgcgcc gccatccgcg tgcgcagggc aatgaccggc gtcaacatct tcatcgagga
    11341 caccggggtt ggcatctcgc cggagaatct tgcacgcttt ggccgaccct tcgatgtcat
    11401 ggagggcaag ctgatcaacg gttgcaaggg gtcgggcctc ggcgtcgcga tcgcccggtc
    11461 gctggtcgag cttcatggcg gtggcatccg tgtccggtcc tgggtcaact ccggcaccat
    11521 cgtgctggtc cacctgccga ttgcgccgcc gcctggaacg cgtgaccctg attcgctcgc
    11581 tgcctgaggc aggctactgc gcggccactg gaacagccga agcccgtggc agggtcagca
    11641 ccgcgcgcac ccccggtgca ttgtcttcga tccgaaagtt tccgccgtgg agacgtgcga
    11701 tcgcggcgac gaggcttaga ccaagcccga agccgggttg ggccctgctc ttctccagac
    11761 ggacaaatcg atccagcacg cgctcgcgct cggctggcgg cacgcctaca ccgtgatcgg
    11821 cgaccaccag cagggcggtt ccaccttcgc agccaagcgt caggcgcact tcggcaggcg
    11881 cgtccgcccc ttcaaccggc accccgtact tgacggcatt gtcgacgaga ttggacagcg
    11941 cctgaccgag cagttccctg ttgccgcgca ccacgacggg cgtttgcgcc tcgaccacaa
    12001 gccgcagcct ggcctcctcc gccaccggat catagagttc accgacatcg cgaacgatct
    12061 tggcaagatc gacgtccgtc atggcgccgg gcgtggaccc ggcctccgcg cgggcgatca
    12121 tcagcagcgc attgaacacc ttgatgagcg cgtcggactc ctcgatcgtc cgctcgagcg
    12181 cttgccgcag atgatcgggc gaacgcgcgt tgcgcagcgc ttcctccgct ccggcccgaa
    12241 gccgggtcag gggcgtgcgc aggtcatggg cgatgttatc ggacaccgcc cgcatgccgg
    12301 acatcagttc gtcaatgcgg gcaagcatct ggttcaggct ctcggccagc cggtcgaact
    12361 cgtcgccgac accgcgaacc ggcaggcgtt ccttgaggtc gccggacatg atgcgccggc
    12421 ccgcctccgc gatgtcctcg acagggcgca gcacgcgccg ggacaccatc aacgcgccga
    12481 aaccaccgag cacgaacacc agcaggatcg aaagccccat tgcctggcgg atcacctgac
    12541 gcaatcgctg gcgctcttcc aggtcatgac cgaccagcag ccgcatgccg ttcggcaaga
    12601 cgaagatgcg cacctttgcc agggtaatga acgactcctg ctcgtcggcg cggcggaacg
    12661 gcacttcccg ccagcctggc ggcgactgca acacctgaac cagttccgtg gcgttgccgg
    12721 ccagaacctc gctgcgggta tcggcgagga gatagagaaa gccgcgggcc tgtcgggatc
    12781 tgcgctcgat ggtcgccgcg agccggcgca tgccaccgat ccggtactgt tcggcgaggc
    12841 tgttgatctc ggtttcgatg gtttcggaaa gctgcgactc gagcaggcgc cgcgtattcc
    12901 aggcgacata gcccagcacc gcgccggcaa agagcacgaa aatgacgaga tagacgaccg
    12961 caaggcgaaa ggccgtggtg cggagtagtt taccgaagcc cgtcacggat catgtatccg
    13021 gcgccgcgca cggtatgcag cagcggctta tcgaagccct tgtcgatctt cgaccgcaat
    13081 cgcgacatat gcacgtcaat gacgttggtc tgcgggtcga aatgatagtc ccagacgtgc
    13141 tccagcaaca tcgtacgggt cacgacctgc ccggcgttgc gcatcaggta ttcgagcagg
    13201 cggaactcgc gcggctgcag atcgatctcg gtggcgccgc gcgcgacgcg gtgggagagt
    13261 cggtccattt cgagatcgcc gacacgatag acagtctgcg ccggggaaga gccgcgccgc
    13321 cggcccagcg cttcaacgcg ggcaagcaat tcggtgaagg catagggctt tgagagatag
    13381 tcgtcaccgc cggccctaag ccccacgaca cggtcatcca cctgaccaag cgcggacagc
    13441 accaggatcg gcgtttcgcg cccctccccg cgcactgcgc tgacgagcga cagcccgtcg
    13501 cgccttggga gcatgcggtc cacaatcatt acgtcgtaag gggcggaaag cgccatttcc
    13561 aggcctttct cgccgtcgcc ggcgtggtcg gcgacatgcc cggcctcacg cagccccttg
    13621 gcgagatagg cggccgcctc cgcatcatcc tcgacgatga gaatcttcat ggtcatgaga
    13681 ctaccacgag aaaagtgaga aggcaggccg gactgggcgg tccgacctgc ctcaaaaacg
    13741 cttcccggat tgggctacct tgggggcgac gggactttgg caggccgtct cgtgaagcat
    13801 cctgaccata ggtggcctgc gattacgcaa acctgtccgg atcatgaatt cttggtaatg
    13861 ctgtcctgcg ccgcaacgat gcggtcgagt tccgcctgct cctcggccga gagcgccgct
    13921 tgcgcgacag gcgcggcgac ctgtcgcccg aacaaccggc gcacgccgaa aatgccggcg
    13981 atcagagcca gaaacggcag cgtccacagc agcaggttct tcatggagaa gacggggcgc
    14041 agcagcacga actcgccgta gcgcgccacc aggaagtcct tgacctggcg atcgctgtcg
    14101 ccggcgctga tgcgctcgcg taccagccga cgcaggtcct tggccagttc ggcgtcggag
    14161 tcatcgatag actggttctg gcagacaagg cagcgcaatt ctgccccgat ttcgcgcgcc
    14221 cgctgctcct gcgccgggtt gggaaggcgt tctgcgggag acaggccaaa agccgggcca
    14281 gacagcacgg caatcatcat cgctgccgcg agaaatcggg cctggcgccg catcattccg
    14341 ccgcctcagg catggcgggc aacttgcgac ggctcggcgc cgcgacgcgc agccggcggt
    14401 ccatgagcga gatcaggcca gcgatggtca tcgccagggg accgagccag atcaggagca
    14461 cccagggttt ccagtaggcc ctgacatcga ccgaggcgcc gtgggcatcg ccgatggcga
    14521 gatagatctg gccaagtcca cgcgtcaatc ttccagtttc ggatgtctgc atggtgcggc
    14581 cgacataggt ccgcttgccc ggttccagaa ccgccaacgc cacgccatcc ttgctcacga
    14641 caaagcgacc gacggttgac gcgaagttcg ggccgggctg cttgcgcagc ccctcgaaga
    14701 cgagttcgta gccgccgatg atcgccttgt cacccggagc catggtggcg atcttctctt
    14761 cgtggaaggc cgtcgcggca atgccgatcg tcatcaggcc gacaccggca tgggcaatgg
    14821 ccgtcgcatg caccgacttc ggcaggccaa cgaagcgccg catccaggtg cccgccgctc
    14881 cccgcgaggc gcgcacgcgc aagtcgtcga gcgcgccgac agtgacgaac atcccgacgc
    14941 cgaggccaaa ggctgccacg gcttcgctcg tgcgggtgat cgcgaggacg gcgacgaaca
    15001 ttcctgcccc gacaaggatc gcgacgaaca gccgctgggc taccggcaga agatcgccgc
    15061 gcttccaggc cagttgctgc ccaaagggca tggcgagcac cagcggcgcg cagagcggca
    15121 ggaaggtggc gttgaagaag ggcggaccaa cggaaatttt gtcgcctgtc accgcttcca
    15181 gcgccagcgg atagagcgtg ccggtcaata ccgcgacgca ggccgccgac agaaagaggt
    15241 tgttgagaac caggctgccc tcgcgcgaga ccggcgagaa caatgcgccg ccggccagcg
    15301 ccggtccgcg ccaggcgaac agggcgaagg ccccgccgat gaacaggcac aggatgagca
    15361 gaataaagac gccgcgcgcc gggtcggagg cgaaggcgtg gacagaggtc agcacgccgg
    15421 agcgcaccag gaaggttccg agcagcgaca gcgagaaggt gagcaccgcc aggaggatcg
    15481 tccagacctt cagcgcctcg cgcctttcca tgacgatggc cgaatgcaga agcgccgtgc
    15541 ccgtcaacca gggcatcagc gaagcgttct ccaccggatc ccagaaccag aacccgcccc
    15601 agccgagttc gtagtaggcc cagtacgacc ccatggcgat gccggcggtc agcgtcgtcc
    15661 aggcgatcag gttccacggc cgcacgaccc gtgcccaggc cgcatcgata cgcccgagga
    15721 tcagggcgcc ggcggcgaag gaaaacacga tcgaatagcc gacatagccg aggtagagca
    15781 gcgggggatg gatcgccagg cctgggtcct gcaggatggg gttgagcgaa cggccttctg
    15841 cgggagcggg aaacacgcgc aggaacgggt ttgacgtcaa cgcgatgaac agcaggaagg
    15901 cgaacgcgat cgaggactgc accgcgatgg tcgcggcccg caatgcctcg ggcatgtgcc
    15961 gaccaaacag cgcgacgcag gcgccgaaca gcgccaggat cagcacccag agcaggatcg
    16021 acccctcgtg gttcccccag acgccggaaa agcgatagat caacggctgg tcggtgtggc
    16081 tgttctcgta gacgttgaga acggagaaat ccgactggac gtagactgcc gtcagcgccg
    16141 cgaaggccag cgccagcaga acgaactgcg ccagcgcggc gggacgcgcc gttgccatca
    16201 gcgcgcggtc ctcgcgcacc gcgccccaca agggcagaac cgtaccgatg gcggagcacg
    16261 ccagcgccag caccagggcg aaatgtccga gttcggcgat cacttcggtt tgccccccgc
    16321 ttcgccgggc ttggcctcgc cctgccagtg cccctgtttc ttgagggcgt cgctcacttc
    16381 gcgcggcatg tagttctcgt cgtgcttggc aagcaccgta tcggcgagga agctgccgtc
    16441 ggcgtgcagc acaccctcgc agaccacccc ctgcccttca cggaacagat ccggcaggat
    16501 gccaacatgt cgcacagtga tggaggcatt gccgtcggta acgcggaagc tgatacgctg
    16561 ccccacggcg cgctccacgg aggcgttctc gacaaggccg ccgagacgga agcgctggcc
    16621 tggcttgatt gccatctgct gcacttcagt cggtgagcgg aagaagacga tccggtcctt
    16681 gagggccgtc aggatgaggg ccacggccag ggcgagtatg gcgccggccg acgcgatcaa
    16741 ggtgatgcga cgctgctttc gcgtcatggc ttggcctccg ccgggagccc gagttccccg
    16801 gcaagcgctt cgatggctgc cgcaccatcc ttgccccgga acgcggcgcg ggcctccgcc
    16861 acggccgcga tcagcttatc cctttcgccc aggacggcga gagcgcgaat gagcttctgc
    16921 cagtcctcgg ccgagccgcc atcctgtttc agccggattg cgagggcgtc caccatgccg
    16981 cggatggcgg tgcggcgctc tgcctccgga agcgaacgga tcgcgtcggc ggcggcgccc
    17041 gtcggaccca ggcgcgcgag attggagcgg atcgcggttt tctcgttgcc gtccggcagg
    17101 tcgtcatgca gcgcctgcca gactgcgcgg gcgccgtcca ggtctccgga ctgctctgct
    17161 gcaagtcccc ggtagaagcg ggcgcgcgcc agtttcggat cgccggcaag agccttgtcg
    17221 atcgcagcct tcgcagcatc gccgacgacg ccgcgtgcgt tgaaaatcag cgcctcggcc
    17281 agcgacgcct gccgctccgg cgtatcgccc ttcaacgcaa tcagccgttc cgctgccgcg
    17341 accgcgttct cgaaacggcc gagccgcatg tacacaggga aggcgagttc ccagcctcgg
    17401 gcatcgtccg ggctgtcagc gaggtgcgca tcgagccggc cgatcaggcg ggtcaactcc
    17461 tcgtcagaac ggttgagatc ggtgcgcgag gcatagggca tgccgggaag ttccgggcgg
    17521 ccctgggtca ggtaaagtga aagggtcaac gccggtatcg ccaggaccgt gagcgcctgc
    17581 gccagccgcc tgcggcttgc atcgttaccg attgcagccg agccgccgcg cttcacgccg
    17641 atgagccgcc gcgctgcttc ggccagcgcg gccttcgcgt cgtcggccgc catcagccct
    17701 tcggcctcca gccgcgcgat ttcagcttca cggctcttgt agaatgcaac gtcgaaatcg
    17761 ccgccgccgg aagccagagg cttcggcgtg atggccatca acagcaccag tacgacggct
    17821 gcgaccatgc ccgcagccag aaaccagaac agcatgccgc tgcacataag gcgtttggcg
    17881 cggaaaggaa acgcgacttt cgcgcacggc cctgcgattt cgttcttgca ggcgccatta
    17941 gaccgcaggc agcaccagtt tcgccctcag cccgccaagc ggcgagtcgt cgagttcgaa
    18001 cgttccatgg tagagggcag caaggtccac gacgatggaa aggccaaggc cggagccggg
    18061 cttggtctca tcgaggcggc ggccgcggcg ggtcgcctgc attcggttct ctggtgtcag
    18121 gcccgggccg tcgtcatcga tgaggacgct gaaccgtggc gcggcgccgg gatccacggg
    18181 aacgggggcg atagagacct cgacgcggct cctcgcccat tttccggcgt tgtcgatcag
    18241 gttgcctatg aggtcttcca gatcctgccg ctcaccccgg aacctcagac ctttcgcgcc
    18301 ggtcgagaca taggcgacgt cgcggtcacg gtagatcttc gggaaggtgc gcaggaatgc
    18361 atcgatcaca ggatcgacct ccgtcaccga gccgatggtg acggcgcgcg tcgcggcgcg
    18421 cgcacgatcg aggtagtact gcacctgatc cttcatgatc ccggtttgct cgcggagctt
    18481 cagtgccagc gccgacgtat cctgctcagc ttcgttgacg agcacgctaa tcggcgtctt
    18541 caatgcatgc gcgaggttgc cgacctgcat gcgtgcgcga ttgacgatct cgcggttggc
    18601 gtcgatcagc tcattgagtt cgtgggccag cggcgccact tcctgcggga aacgttcgcc
    18661 gacatgctcc gcgtcgccct ggcgcacggc aaagacggcc cggcgcaatt cggccagcgg
    18721 catcaggcca tagccaacct gcaccacagt ggacccgatc agagcagcgc cgagcagcac
    18781 gaatgtgatg gtcagggcgg tattgaaccg gttggtttca tcctggattt cagccgcgtt
    18841 cccggcgact gaaatgtaga atcgattgtt gtcgccaagg tcgatcagct gttcgaggat
    18901 gcgcaacctc ttgctttccg gcccggtgat gtagccatcc gaagcatcgt tctctcgcct
    18961 ggggtctatc gggtcaataa ggaagggcag cttggtggag aacagcgagc gcgaggtgcg
    19021 catcaccgcc gcagtgtcct tttcctgatc aagtttgacg atctgccagt accagcccga
    19081 aagaggaatc tggaactgcg gttcgccgag attgcccaca tcggtggatg tcccgtcagt
    19141 cagggtggcg acgtcagcaa ctagcgcctt caggtagaga cggagccgct cgtcaaaggc
    19201 ctgtgtcacc gaacggcggt tgagttcaga caacaccagc cccgcgatca gcaggaccgt
    19261 gcagcataac agcgtggcgg aaagcaggag gcggccggag agcgagcgga tccgcatcca
    19321 ggattagcct gacttggcct tcccgggctg gccgacgagg tagccgaggc cgcgcactgt
    19381 ctcgatgatg tcgacgccaa gcttcttgcg caagcgaccg atgaacacct cgatggtgtt
    19441 ggaatcgcga tcgaaatcct gatcatagag gtgctcgacc agttccccgc gcgagaccac
    19501 gcgcccctgg tgatgcatca ggtaggccag cagccggtac tcatgggacg tcagcttcac
    19561 gggattgccg tcgacaatca cccggctcga acgcgtatcg agcagcaccg gcccgcagga
    19621 aagttcactg gtggcatggc cggccgcccg gcgcaccagc gcgcgcaggc gggccagcac
    19681 ctcttccata tgaaagggct tggtgacgta gtcgtccgca ccggcatcga acccttggac
    19741 cttgtcgctc cagcggtcgc gcgcggtgag gatcagcacc ggcatgcgcc gccccccctt
    19801 gcgccacttt tccagcactg aaacaccgtc catgcgcggc agtccgagat cgagcacgat
    19861 ggcgtcgtat ggctcggtgt cgccaaggaa atggccttcc tcgccgtcga aagcggtatc
    19921 aaccgcatag cccgcgttct gcaacgcagt cacgagttgg cggttcagat ccttgtcgtc
    19981 ttccacaacg agcaggcgca tggtcgttca cgttctcccg gaagggaatc cctcgaaagc
    20041 atacagcagg caccgccgac gcgacaggaa attgcgccgg cggcgactgc gatgcgacag
    20101 ggtccctgaa ctcggcctga acgggctcta gtcgatcctg cgcgtggccg ccacgcggta
    20161 catggtcgaa gccacgaaac tggcgccggc gaccgcgttc aggatggcct cgaccactgc
    20221 attctgatcg atcatgcccg tgttgaaccc gagcatcgaa gccgcgacgg ctgtcagtcc
    20281 caccagattg gcccagacgg tgcggctttc gaacatggat ttggtcggtg tcatggcctg
    20341 cgttttcctt gtagtggtgt tggaggtgtt ggaggcgatg gatggtgatg gctgtcccgt
    20401 tgccatggcg agcgcggcgc gctccaccgc cgcgacgcgc gccatccagc cacgcccgaa
    20461 aacggcgaaa atgcgcagct tgccgagaaa gccccgccgt tcggcgcaca ccgcgcggat
    20521 cgtcgcctgt ggcgtgtccg agcgcgcagt ggcaagggcg cgaagcgccc gtttcggacc
    20581 ggaattgacg gcatagtcga acgtcgcgag atcgacgccc ggcgccaggt ggtcgccgcc
    20641 gatggcgtcc cagtagcgct ggcgatagat cgccgcggct tcggctcggg ttagtccgcg
    20701 cacctccgcc ttgctgaccg gcctgccacg ccaggcggca agcacggcgc gggtgatgcc
    20761 gaggttggtc gcccccccgg ggtcggcggg gtggtcggca aatccccctt catgcgccag
    20821 tgtgtgcgcg agacaccgct cgaaattgcc cttcgccatg gtcgcctcat ccagcccgcg
    20881 cgcggtcgaa cagcgccttg agatcaaccc ccgccgccgc cgcgatgcgg ttcagcttgg
    20941 gcgatgtttc cggcgggttg ggttccgata cccaatagtc ccgcgtgccc tgcgacatcg
    21001 ccgcgagagc ggtatcccat gcggccctct ggccaatctc attgagcgca tagcgcagcg
    21061 cgagagccga ggcgcagcgc accggcgcgg gaggcgcttc atagggcgct atggcaaccc
    21121 cggactccag cagcgccgcg aaggcctcgg gatgatcggg cgccgaaagc gccaccgccc
    21181 cggcgctgtc ggtggcgatg atcgccgccg tcccctccag gttggcgtaa cgtgcggata
    21241 ggaccttcat gcctgcacct cgctcacgat cagggtgacg cccatcgagc cgccgagctt
    21301 gcggccggaa gacagcccgt tggcatagat cgccgacgag ccgccaacgc gcacgctcca
    21361 ggtctgggat gacgtgctgg acgccgcctg cgcccgctcc atggcgagaa cggtcgggta
    21421 tccgttgccg ttgatggtcg acatgacggt gccgatcgca gcggagccgc cgttgaacag
    21481 cgcggcatag gcgtagatcg ccgaggtgct gtcgaacggc ccggcaaatc gcgcatggat
    21541 gcggttcgcg gccgaggtcg gcgtcagggt cgcgctgacg atctgcgtcc cttccgtgct
    21601 ggtcggcacg gtgtcgtcgt tcggaatgga agtcgaaagc gccgcattgg ccgtgtagcg
    21661 gtccgaagcg atctgcacca gatcgcccgg cagcttcatg ccggcggaat agaccgcgac
    21721 gttcgtcggt gcggtccatg tgccgagcgt cgccagtccg gaagaccatt ccagacggcc
    21781 gagaacgaca tagggcttag aggcgaccgc cgcgcctgtg tagaacacat gggcgctgtc
    21841 cgcgccgccc gcgccacctt ccgccgtgga tgaggcgatg ggcgagccgc gcccgagcgg
    21901 aaagatgttt tctccggaca ggcagttgat gatcccgagg cgcacggtcc cgccatcgtc
    21961 aaaggcgacg atccacagcg cgaacggcgt gttgatggct gcaacgccca gggtggagcc
    22021 ggatgagagc gtcaacgagg ttggggttgc cacctcgcgc gttgcgacgc tgccggaact
    22081 ggccgtagcg gaccggaacg gtatcatgac cgtgtcggag cccgatggcg cggcaagtgc
    22141 cgccgtcttc aggtcaaccg tcagcgcgcc gcctgcaacg ctggctgaca agccgacatt
    22201 caccagcgcg ttgatgccgc ttgccgctcc gccgccgcca cccccggcgc cggtggctcc
    22261 gaaagggaag tccaccacgc cgctcgtcgg gtcaatcacc agcgcctgtt tccaggtcgc
    22321 gccatctgcg gacaccttga tggcgtactg ttcatcgccc atcagtccgg tttccgcccg
    22381 tccggaataa ccggtctggt acagctggct caccgtattc gcggcagcat ccttgttcag
    22441 cttgaaacgg aggtccccgg taccggacgg cgtggattcc gccgcgaaga gcgcattgtt
    22501 gaccttcacc gcaagagggt tggcgccatc cgccatcgtg ccgagaccga gcttgaccag
    22561 gttctggagt tcggtgatgc cgcctgtcga cgacgtagcg ctccagccag agccgtcgaa
    22621 ggcaagcaag gcattctccg cgatcacgta ggcgcaccag ccaacgcccg gcgccaggaa
    22681 attccagccg ccggcatcga agcttgcgac cgcgccggca tgccccacga aggccccggt
    22741 cggcgcggca ccgacgatgt agcggtcgcc attcgccggg gcagccggag gcgtgtcgcg
    22801 gttgcgttcg atcaccgcga gctggacgat cgcgtcgagc cgcagcagcg cctcgttgac
    22861 ggtgacgtgc ttctgcgcct ggcctgcggc ggtcagttgc aggccgagca agggcgtggt
    22921 ttcgctcatg gggtcagtcc gttcgtgttg gggacattca gcggatgggc acgatgccgg
    22981 cgaatggcat gccgtcgccg acggcacggc taatctgcac caaccggacg ttcagcgcgg
    23041 cttgcggcgc accgaaatcg gcgagttcgt ccgcggcggc ataggcgcat gacggctggg
    23101 tcgcggcgat cgagcgcagc agcgcgcccg ccatgctgcg aatgtccagg cgataggctt
    23161 cgctctcctc gccgagcgga acgtcgagcg cttcccagtt gtcgcccccc cgccgcgttc
    23221 gccggatcca gtcgaatgtg ac
//
LOCUS       DAOMXN010000016        22802 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_1386_length_22802_cov_11.355695, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000016 DAOMXN010000000
VERSION     DAOMXN010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 22802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22802
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1386_length_22802_cov_11.355695"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1062
                     /gene="glmU"
                     /locus_tag="PK812_02955"
     CDS             <1..1062
                     /gene="glmU"
                     /locus_tag="PK812_02955"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HRJ68541.1"
                     /translation="GFDAIVVLFADTPLVRAATVQRLAEVAHGGAVAAMCFRAKDPTG
                     YGRFLMDGENVAAIREQKDATDEERRITLCSGGLFGIPGQSALAILEAIGCQNAQNEY
                     YLTEAVSVARGRGLAVRPVEVDEEEAFGVNDRAQLAYAEQVLQGRLRRAAMLSGVTMI
                     DPSSVYLSMDTVLARDVTVEPGVFFGPGVRVEEGAVIHAWSHLEGATVGRGASVGPFA
                     RLRPGAQLGEQVKVGNFVEVKAAQVEAGAKISHLSYIGDARVGAEANIGAGTITCNYD
                     GFRKHRTDIGRQAFIGSNSSLVAPVSIGDGAFVASGSVITDNVENDALALGRGRQAVK
                     SGWAVAFRARPENIAKKKD"
     gene            complement(1081..1692)
                     /locus_tag="PK812_02960"
     CDS             complement(1081..1692)
                     /locus_tag="PK812_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68542.1"
                     /translation="MTVRITNGFGSMPAPQADADRARVFTSLVMAENRSGAQARAHQA
                     QPWAGQLNGKPCSAQPQGPRAANGDQMWRVKWLPDGRTPVQVDTALREQFGPQHVQPG
                     TPVRRALETQWGANQSGPRPWSSADGFMGRPVEAKPVRRVPGTDKHVWEVSVGAPATQ
                     KGGLASMLQKPATATVILDGAFDARCLSQEHPLGRELKAKLGA"
     gene            complement(1878..2741)
                     /locus_tag="PK812_02965"
     CDS             complement(1878..2741)
                     /locus_tag="PK812_02965"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRJ68543.1"
                     /translation="MSGSEPTSSPLIEEIRRVGEGLGPAAQRVAAFIIENATRVPDLS
                     ITDVARAANVSEPTVARLCQSLGYSGFRELRIVLARQQPDSMPIVQIEISARDRPNEI
                     ATKVIDRVLSSLMKLKSELDYAAISAASILLAEARRIEFYAHGESAFLAQDAQLRFAR
                     LGIPSIAYSDSHQHVMSASTLGPRDAVVCISRSGANAELVQTLAILGENAVPVVGIMP
                     RDTALGAYCTQLVHIGADDEPNVYAPKAYRFSQMATIEALAIATAVMCGPSATMNLER
                     TRAASLGKLLD"
     gene            complement(2885..3526)
                     /locus_tag="PK812_02970"
     CDS             complement(2885..3526)
                     /locus_tag="PK812_02970"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HRJ68544.1"
                     /translation="MKLIDRLRQHRLRKDERGLAATEFALIAPLLITIFIGLIEASNY
                     VYANQKVESSAANVLNIINQQSQPSLDDIQRIAMAVPEVAKPLQVGGGDFRVVYTAIQ
                     RDKPGRTADVFPEPYVLWQESYGNPGLGSSVLRYSRGGSKEQNKLNPGDLQGLQFTPG
                     DQVIAVEVYIRYKPTIQTGFSPKYDTAMYYLNFSRPRKGSFSMSPREKSELTN"
     gene            complement(3513..4001)
                     /locus_tag="PK812_02975"
     CDS             complement(3513..4001)
                     /locus_tag="PK812_02975"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HRJ68545.1"
                     /translation="MVRRSFIRRFRRDERGATAIEFAFVAPVLIFLMFSIIELGFMLY
                     LRSAVETATQIVARRAITGNFAGEIPANRQQLFNDMLRQEVGQFLLANTTLQVTTTIW
                     SSLSQPSRVGGGNIGGANQIVRYQVDCTYKFMTPLIGWLNSGSSDNLHIQATAFVKNE
                     AY"
     gene            complement(4018..5406)
                     /locus_tag="PK812_02980"
     CDS             complement(4018..5406)
                     /locus_tag="PK812_02980"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HRJ68546.1"
                     /translation="MSIDPRPAGHKRLLSRLLSAGARLPADRQGSSAIIVALALPALI
                     GFAGLAVDTTRFLRAKTIVANAADHAALAAASVDGEDRVAVAERFFNAQVPESYRQAV
                     SIKPGGVKVRSSTGNGPVIVEVDIEAEVQTILGQVVGIHKLGVQHRAQAKREVNNLEV
                     VMGLPASGTMCSNKGRTPNTNRKVPGDTLLELTPDPGCRHFNAMKTGVKAFIDIVKAN
                     QATASFKVGLVPYNAKVKFANPTMIPPSVGQSEAQGYYADFNGFEPLGAIQSMTSNTT
                     LLQQALDRMTLTPEAVAWTRTDLPTHTAGLMLDPTQRTYFPGAEEVKPYNTRDIKKVL
                     ILMSDGSNIGCCFTNWRPGNFYNQYVYNYKPYNDEQIRICKFLKENNVQIFTILFDVQ
                     ESDVGGDIINNVFARCASGAYSDATAREDNPSAMLRCKHRQNCYNVATDEQLVAAYKD
                     IAQNFYAPVLRK"
     gene            complement(5396..6643)
                     /locus_tag="PK812_02985"
     CDS             complement(5396..6643)
                     /locus_tag="PK812_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Hint domain-containing protein"
                     /protein_id="HRJ68547.1"
                     /translation="MRRLRPIAATFALLGLVATAQAQLAWKAVTPSCLPVPGKMMRWS
                     NPQNIPACHCPPKDFCPTTMDEWTSEARMPSFMAEMCCDRPYQCTVEWIHNGGGPCPS
                     YFRAGPEKAEFCNHYKPLQTSDNKEKERRANAAKDLNACLAKKCGPLQKALLDAAPKH
                     NTGVTSPNTKALADYALCEITCRTDAATALGIAVNTIDRSFYDTAAKLSTTFNGVQMP
                     SRVAAFMQTRQLPSPWKVIPAGQGLESKLTCDDKQSSNICLGAMVQTRSTCGDCLAAG
                     TRIQMADGSEKVIEDIRAGDVVAGLGRTNTVLELVSLDWSRVILHRINGAITLTADHP
                     VMTTTGWKAIDFEDGAFKYGLQDVPKLVVGDTLVTLNGSVRVETIEAVPETDGYRTYN
                     LRTRGNGTFIANGLIVKDSVGVD"
     gene            6855..7637
                     /locus_tag="PK812_02990"
     CDS             6855..7637
                     /locus_tag="PK812_02990"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HRJ68548.1"
                     /translation="MSTEVLRLIVFAAILTSLMVVEHFAPWRGQAQRLRRWPSNLGVL
                     LIDSALVRLVFPVTAAGFAELCALKGWGLFNLLAVPGWIAFVLSAVALDLAIYLQHRV
                     FHHVPALWRLHRMHHADTMLDATSGTRFHPLEILLSMAIKLAAIAVLGAPAAAVILFE
                     ILLNATAMFNHANIVLPTRADRVLRWFLVTPDMHRVHHSSERIETDSNFGFSLPWWDY
                     LFKTYNERPEKGLDGMEIGLEVFRDPGEQRLDRMLTQPFRDP"
     gene            7791..9617
                     /gene="glmS"
                     /locus_tag="PK812_02995"
     CDS             7791..9617
                     /gene="glmS"
                     /locus_tag="PK812_02995"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HRJ68549.1"
                     /translation="MCGIVGILGVGPVAPQVVDSLKRLEYRGYDSAGIATLENGHLTR
                     RRAEGKLRNLQALLTLEPLAGHIGIGHTRWATHGRPSEYNAHPHATDRVAVVHNGIIE
                     NFRELRQELTGEGVNFSSDTDTEVVAHLVTRELRKGASPRDAVAFALKRLHGAFALAF
                     LFEGEENLLIGARQGAPLAVGYGDGEMFLGSDALALAPFTDVISYLDDGDWVELTRNS
                     AVFHDASDAIVDRPRQKTQAGAFLVDKGNHRHFMAKEIHEQPEVIGRTLAHYLDMAAG
                     RVNLPFALPFDFRTLRKVSISACGTAFLAGLTAKYWFERIARLPVEIDIASEFRYREM
                     PLDKGELAIFISQSGETADTLASLRYAKACGQHILSVVNVPTSTIARESDVVALTLAG
                     PEIGVASTKAFTCQLSVLAALAVAAGAARGTIDATREHKLVSDLMLIPGLVADSIKRE
                     AQVEQIARELTKAKDVLYLGRGTSYPLALEGALKLKEISYIHAEGYAAGELKHGPIAL
                     IEETLPVIVLAPRDQWFDKTVSNVQEVAARGGQLLLIGPKGIADQAGLTSAESIVMPD
                     MEPDFSAIVYAIPVQLLAYYTAVHMGKDVDQPRNLAKSVTVE"
     gene            9856..10260
                     /locus_tag="PK812_03000"
     CDS             9856..10260
                     /locus_tag="PK812_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68550.1"
                     /translation="MTGAVMRAVRQTSPQLQVVPKAEPQFDVTFKPVMTRREAEILFE
                     IDSVQNGDNPAWTAFFVESLVEFVVYGSRPTGQVSDTDAQWIVSCVGTEASASVPALL
                     RAIILQAENVPGCLIHLAMRCGAMRGPSAVLI"
     gene            10338..12350
                     /locus_tag="PK812_03005"
     CDS             10338..12350
                     /locus_tag="PK812_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003599287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HRJ68551.1"
                     /translation="MFGKILIANRGEIACRVMKTANRMGIKTVAVYSDADRDALHVAM
                     ADEAVHIGPPAAAESYLVIEKIIAACKQTGAEAVHPGYGFLSEREAFAEALKAANITF
                     IGPNPRAIAAMGDKIESKKFANAAKVSTVPGYLGIIDGPEQAVTIAEEIGYPVMIKAS
                     AGGGGKGMRIAHSAHEVADGFARAKSEAKASFGDDRVFVEKFITDPRHIEIQLIGDKH
                     GNVVYLHERECSIQRRNQKVIEEAPSPLLDAATRKKMGEQAVALAKAVDYDSAGTVEF
                     VAGQDRSFFFLEMNTRLQVEHPVTELITGIDLVEEMIRVAAGEKLRFTQSDIKLDGWA
                     VESRVYAEDPARNFLPSTGRLVTYRPPTEGFFDGVKVRNDTGVFEGGEISIYYDPMIA
                     KLVTHAPTRLGAIEAQAEALDAFAIDGIRHNIPFLSTLMQHPRWRSGALSTGFIKEEF
                     PEGFVAPHPEGEIRDRMAAVAAVLDHQSNHRRRQISGQIDGARTDRIQRVRAVMLGDE
                     RVNLETEMTNSGFTVTFLDGNKRRSVKVVPQSLPGELVWRGTFDKTPVAVQVRAVNNG
                     YRLSHGGAAAVVYVYTKREADIFALTPKKQKADTSKTLLCPMPGLVKQIAVKEGQEVK
                     AGDILAVVEAMKMENVLRAEKDVTVKSVKAKEGDSLAVDAVIMEFA"
     gene            12347..12727
                     /locus_tag="PK812_03010"
     CDS             12347..12727
                     /locus_tag="PK812_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HRJ68552.1"
                     /translation="MTLARSGFAPGIHEIEAYGRGGFRFGGVSHIGSILATPSGIHAW
                     SPRSAREITPEVLEALAADAARIDLLFLGTGRDLVPMSAPVREHLASIGLRFEALPTA
                     AAARTYNVLVREGRRAAAGLIAVD"
     gene            12796..13671
                     /locus_tag="PK812_03015"
     CDS             12796..13671
                     /locus_tag="PK812_03015"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HRJ68553.1"
                     /translation="MNFQAPKSIMLEKAYAACCEHVRTLDADRYLATLFAPAERQPHI
                     FALYAFNAEIARVRGLVSDPLPGEMRFQWWRDLLHGEAQGDAARNPIAIALVATIQTC
                     KLPVKPFTDLLEARTFDLYDDPMPTWLDCEGYCGETSSALIRLTSLALGGGADNDAAI
                     AAGHAGVAFALTGLLRAFPWTCRRGQLFLPKEVFAAHGVDLADLPQGKDSSGLRAALG
                     EVRERAMDHLRKTRELIGSVSPRIAPAFFPCCLVEPYLRQMEAKDYDPFRTRIDVSRL
                     LKLFTLWRQSRRSRG"
     gene            complement(13685..15112)
                     /gene="trmFO"
                     /locus_tag="PK812_03020"
     CDS             complement(13685..15112)
                     /gene="trmFO"
                     /locus_tag="PK812_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012171413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HRJ68554.1"
                     /translation="MSKTLTSLSPIHVIGGGLAGSEAAWQIAVSGVPVVLHEMRPERM
                     TDAHKTEGLAELVCSNSFRSDDWQTNAVGLLHAEMRRMDSLIMRSADAHQVPAGAALA
                     VDRDGFSQAVTAALAAHPLITINRGEIAGLPPADWDSVIIATGPLTSPALAQGIRDLT
                     GESGLAFFDAIAPIVHFDSVDMKKAWFQSRYDKAGPGGTGADYINCPLDREQYEAFVD
                     ALLAGDKTEFREFEKNTPYFDGCLPIEVMAERGRETLRHGPMKPVGLTNPHNPTVKPH
                     AVVQLRQDNALGTLFNMVGFQTKLKYGAQSDIFRLIPGLEGAVFARLGGIHRNTYLNS
                     PRLLDNRLRLRADPRLRFAGQITGCEGYVESAAVGLIAGRLAAAERHGRVMAPPPATT
                     AIGALINHITGGHIDTIDAGPRSFQPMNVNFGLFPPLDSAPRSPDGKRLRGKDKALAK
                     KKALTARAMADLAGWCDAHVAPGER"
     gene            complement(15248..15406)
                     /locus_tag="PK812_03025"
     CDS             complement(15248..15406)
                     /locus_tag="PK812_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009492360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HRJ68555.1"
                     /translation="MILSVILARIQTWLKVRASMRELNALSDRELADIGVNRSCIEQV
                     ARECSAAH"
     gene            complement(15591..16166)
                     /locus_tag="PK812_03030"
     CDS             complement(15591..16166)
                     /locus_tag="PK812_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68556.1"
                     /translation="MRIKSIFAKTAIATAFGFGVALGSITLADELPLKPAGANAQNQK
                     ATKVAAVPPAVAAAAKVAQGSNMRIVVVEWKIKKGREQEFLDYWAERSTIPNRDGLVA
                     EFLSTVDSKEKWPWINWLGPIDKPGITTFYNVGLWRDGESFQDNIGKFIDNNRPPLDF
                     EGEKRTRVFLAPQRWRVGETRVPAKDSDGTK"
     gene            complement(16442..19408)
                     /gene="uvrA"
                     /locus_tag="PK812_03035"
     CDS             complement(16442..19408)
                     /gene="uvrA"
                     /locus_tag="PK812_03035"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRJ68557.1"
                     /translation="MTPPASGSARARRDADKTPVDSLFDRAKAAGGSVDGRVIAIRGA
                     REHNLKNVDLVIPRDKLVVFTGLSGSGKSSLAFDTIYAEGQRRYVESLSAYARQFLEM
                     MQKPDVDQIDGLSPAISIEQKTTSRNPRSTVGTVTEIYDYMRLLWARAGVPYSPATGL
                     PIESQTVSQMVDRVLALPEKTRLLLLAPAVRGRKGEYRKELAEFLKRGFQRVKIDGAM
                     HEIADAPALDKNIKHDIDVVVDRIVVRPDIGARLSESLETALELADGIAVIEWVDEQT
                     ADGSPRRQIFSSKFACPVSGFTIPEIEPRLFSFNNPFGACPHCGGIGSELKIDADLIV
                     PDASLTLKKGAIAPWAKSTSPYYGQTLDALAKHYGFSLTVPWEKLPKQAQNVILYGSG
                     GETIRMAYNDGLRSYEVKKPFEGVIINLERRFKETESDWAREEIGRFMGETPCLTCEG
                     HRLKPEALAVKIAGRHVGEVTRLSVKDAADWYNTLPERLTAKQNEIAGRILKEIRDRL
                     RFLVDVGLEYLTLARSSGTLSGGESQRIRLASQIGSGLTGVLYVLDEPSIGLHQRDNE
                     RLLGTLKRLRDLGNSVIVVEHDEDAIRQADHVVDVGPGAGIHGGRIVSQGTPEEVIAD
                     PKSLTGQYLSGTKSVPMPKKRRVPQKSRMLRVVGARGHNLKSVTAEIPLGLFTCVTGV
                     SGGGKSTLIIETLFKAAARRLNGALEHPAPHDRVEGLELLDKVIDIDQSPIGRTPRSN
                     PATYTGAFTPIRDWFAGLPEAKARGYQPGRFSFNVKGGRCEACQGDGVIKIEMHFLPD
                     VYVTCDVCKGKRYDRETLEVKFRGKSIADVLDMSVEEAAEHFKAVPPIRDKMETLARV
                     GLKYISVGQQATTLSGGEAQRVKLSKELSKRATGRTLYILDEPTTGLHFHDVAKLMEV
                     LHELVDQGNTVVVIEHNLEVIKTADWVIDLGPEGGDGGGRIVAQGPPEAIARAEASHT
                     GRFLDDVLKRRPRATS"
     gene            19598..20083
                     /locus_tag="PK812_03040"
     CDS             19598..20083
                     /locus_tag="PK812_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRJ68558.1"
                     /translation="MAGSLNKVMLIGNLGADPEIRRLPSGGSVANMRVATSESWRDKN
                     TGERREKTEWHNVVVFNDNLVRVIEQYIKKGAKVYVEGQLQTRKYQDKSGQDRYSTEV
                     VLQGFNGQLTMLDGRGEGGGRDGGDDRGGSDFGRSSPMERRPAPAGGGRRSDMDDDIP
                     F"
     gene            20227..>22802
                     /gene="gyrA"
                     /locus_tag="PK812_03045"
     CDS             20227..>22802
                     /gene="gyrA"
                     /locus_tag="PK812_03045"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014889727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRJ68559.1"
                     /translation="MADDDKKPDDPSEVHPVSITDEMRRSYLDYAMSVIVSRALPDAR
                     DGLKPVHRRILYSMHENGYTPEKAYKKSARVTGDTMGKYHPHGNLAIYDALVRMAQEF
                     SMRLPLIDGQGNFGSVDGDPPAAERYTEVRLEKVALALLDDLGEDTVDFQSNYTGEFD
                     EPMVLPAKFPNLLVNGAGGIAVGMATNIPPHNLGEVIDACNALIADPALGIDELMRLV
                     PGPDFPTGALILGRGGIRSAYTTGRGSIIMRARASVEEIRKDREAIIVTEIPYQVNKA
                     TLIERIAELVREKKLEGISDLRDESDRQGMRIVIELKRDAMGDVVLNQLYRHTALQTS
                     FGANMIALNGGRRPETMNLKDMLVAFLDFREEVIGRRTRFRLNKARDRAHVLVGLAIA
                     VANIDEVIALIRGSADAATAKAALMERAWPAHDVAPLIALIDDPRHGLTAEGTYRLSD
                     RQAQAILDLRLQRLTALGRDEIAEELEKIAAEIREFLDILASRERILGIVQTELNEVR
                     AAFATPRRSEIIEADSDFEDEDLIAREDMVVTVSHGGYIKRVPLSTYRAQRRGGKGRS
                     GMQTKEEDFVSRLFVASTHAPILFFSSLGQVYKMKVWRLPEGAPQSRGKALVNLLPLD
                     PGERITTIVPLPEDEGQWTALDVMFATTTGSIRRNKLSDCVPRTSLGKRVMEFDGPEQ
                     TIVDVALATEADDVLLTTAQGQCIRFAVTDLRVFGGNGSTGTSTGVRGINLGGDDRVI
                     SMSILRHVEATPEERSTYLKQARLTRGEAGEAEAAADDEVADSAGNLPPERYAEMSAL
                     EQHVLTISENGYGKRSSSYEYRITNRGGKGIVAMAVNERNGALVASFPVENADQIMLV
                     TNG"
BASE COUNT         4002 a         7179 c         7372 g         4249 t
ORIGIN      
        1 ggcttcgatg cgattgtcgt gctgttcgcc gataccccgc tcgtgcgcgc cgcgacggtg
       61 cagcgcctgg ctgaggtggc gcatggcggc gccgtcgcgg cgatgtgctt ccgcgccaag
      121 gacccgacgg gctatggccg cttcctgatg gatggcgaga atgtcgcggc gatccgcgag
      181 cagaaggacg ctaccgatga agaacggcgc ataacgctct gcagcggcgg tcttttcggc
      241 attccgggcc aatcggcgct ggccatcctt gaggccattg gctgccagaa tgcgcagaac
      301 gaatactacc tgacagaggc ggtttccgtg gcgcgggggc gcggccttgc tgtccgcccg
      361 gtcgaggtgg acgaggaaga ggctttcggt gtcaacgatc gggcgcagct ggcctatgcg
      421 gagcaggtct tgcaggggcg gctgcgccgc gccgccatgc tctccggcgt gaccatgatt
      481 gatccgtcca gcgtctatct gtcgatggac acggtcctgg cgcgcgacgt caccgtcgaa
      541 cccggcgtgt ttttcgggcc gggcgtgagg gtcgaggagg gcgccgtcat ccatgcctgg
      601 tcgcatctgg aaggcgccac ggtcggacgc ggcgcctcgg ttggcccctt cgcccggttg
      661 cgtccgggcg cgcagctcgg cgaacaggtc aaggtcggca atttcgtcga ggtcaaggcg
      721 gcccaggtcg aggccggcgc caagatcagc catctcagct acatcggcga tgcccgcgtc
      781 ggcgcggaag ccaacatcgg cgccggcacc atcacctgca actacgatgg tttccgcaag
      841 catcggacgg acatcgggcg gcaggctttc atcggctcca actcgtcgct ggtcgcaccg
      901 gtcagcattg gcgacggggc cttcgtcgct tccggctcgg tcatcaccga caatgtcgag
      961 aacgatgcgc tggcgctggg acgtggccgt caggcggtga agtcgggctg ggcggtcgca
     1021 ttccgcgccc ggccggaaaa catcgccaag aagaaagact agccaggtcc ggcgcagccg
     1081 tcaggcgcca agcttggcct tgagttcgcg cccgagcgga tgttcctggg aaaggcagcg
     1141 ggcgtcaaac gccccatcca ggatcaccgt ggccgtggcc ggcttttgca gcatggaggc
     1201 gaggccgccc ttctgcgtgg ccggtgcgcc cacagacact tcccagacat gcttgtcggt
     1261 gccgggaacg cggcgaaccg gcttggcctc gaccggacgg cccatgaaac cgtcggcgct
     1321 ggaccatggg cgcgggccgg actggttggc gccccattgc gtttccagcg cccggcgaac
     1381 cggcgttccg ggctggacgt gctgcgggcc gaactgctcg cgcagcgcgg tgtcgacctg
     1441 aacgggcgtg cgaccgtccg gcagccactt cacccgccac atctgatccc cgttcgcggc
     1501 gcgcggtcct tgcggctgcg cgctgcaggg cttgccgttc aactggccgg cccagggttg
     1561 cgcctgatgc gcgcgggcct gcgcgccgga gcggttttcg gccatgacca gcgaggtgaa
     1621 cacgcgcgca cggtcagcat ccgcctgagg ggcgggcatc gaaccgaagc cgttggtgat
     1681 cctgactgtc atgccaatcc aaacatgtcg cgccatgccc gcaagcgcag gcatcgtctg
     1741 caacaggcct tctagcaaac cggcgcctgt atggaaagcc cttacatggg aaaacggtcc
     1801 atgcggttaa gccgcatttg gagccggttg ccgccggcgg cgctcacttc cggcgggcgc
     1861 gggatcttcc cgtcccgcta gtcgagcagc ttgcccagcg aggcggcgcg cgtgcgctcc
     1921 aggttcatgg tggccgaggg gccgcacatc acggcggtcg cgatggccag cgcctcgatc
     1981 gtcgccatct ggctgaagcg gtaggccttg ggcgcgtaga cgttcggttc gtcgtcggcg
     2041 ccgatatgta caagttgcgt gcaataggcg ccgagcgccg tgtcgcgggg catgataccg
     2101 accacgggca cggcgttctc tccaagaatg gccagggtct gcacaagttc cgcgttcgcg
     2161 cccgagcgcg agatgcacac gaccgcatcg cgtggcccaa gcgtcgaggc cgacatcaca
     2221 tgctgatgcg aatccgaata ggcgattgat ggaatgccga gccgggcgaa ccgcagctgc
     2281 gcatcctgcg ccaggaacgc cgattcgccg tgggcgtaga actcgatgcg gcgtgcctcc
     2341 gccagcagga tcgaggcggc cgagatcgcg gcatagtcca gttcggactt cagcttcatc
     2401 agcgaggaca gcacccggtc gatgaccttg gtggcgattt cgttgggccg gtcccgcgcc
     2461 gaaatctcga tctggacgat cggcatggaa tccggctgct gccgcgccag cacgatccgc
     2521 aactcgcgga agccgctgta gccgaggctc tggcacaggc gcgccacggt cggctcgctg
     2581 acattcgccg cgcgtgcgac gtcggtaatc gaaaggtcgg gaacgcgggt ggcattctcg
     2641 atgatgaagg cagcgacccg ttgggccgcg gggccgagcc cttcgccgac gcggcggatt
     2701 tcctcgatga gcggggagga tgtgggttcg ctgccagaca cgtgtcggtt ccttttccgc
     2761 ggcaggaaag aagtttccgc tgcatcgggc aggaatctac aggagcggac gcgcgaacaa
     2821 aagccctgtc cgatggttgc gaacagggct tgtcgccctt gacggcgtca gcggaccgtc
     2881 gtggtcagtt cgtcaactcg gatttttcac gcggcgacat cgagaacgag cctttccgcg
     2941 ggcgcgaaaa attcaagtag tacatcgccg tgtcgtactt tggcgaaaat ccggtctgga
     3001 tcgtcggctt gtagcggatg tagacctcca ccgcgatgac ctggtcgccg ggcgtgaact
     3061 gcaggccctg caggtcgccg ggattgagct tgttctgctc ctttgaaccg ccgcgcgaat
     3121 agcgcagcac cgagctgccc aaacctgggt ttccatagga ttcctgccag agcacatagg
     3181 gttcggggaa aacgtcggcg gtgcgccccg gcttgtcccg ctggatcgcg gtgtagacca
     3241 cgcggaaatc cccacctccg acctgcagcg gcttggccac ttccgggacc gccattgcga
     3301 tgcgctggat gtcatccaga gacggctggg attgctgatt gatgatgttc agcacgttcg
     3361 cggccgagct ttccaccttc tggttggcgt agacatagtt cgaggcttcg atcaggccga
     3421 tgaagatcgt gatcaggagt ggtgcgatca acgcgaactc ggtcgcggcc agtccccgct
     3481 cgtccttgcg cagccggtgc tgtctcaggc gatcaataag cttcattttt cacgaacgcc
     3541 gttgcttgga tgtggagatt gtcggaactg cccgaattga gccagccgat gagcggtgtc
     3601 atgaacttgt aggtgcaatc gacctgatac cggacgatct ggttcgcacc gccgatattg
     3661 ccgccgccga cgcgactggg ttggctgagg ctgctccaga tcgtggtggt cacctgaagc
     3721 gtggtgttgg cgagcaggaa ctgcccgacc tcctgacgga gcatgtcgtt gaagagttgc
     3781 tggcggttgg ccggaatctc gcctgcgaaa ttgccagtga tcgcgcggcg cgcgacgatc
     3841 tgcgtcgcgg tttccaccgc cgaccgcaag tacagcatga acccgagttc gatgatcgag
     3901 aacatcagga agatcaggac cggagcgaca aacgcgaact cgatcgccgt ggcgccccgc
     3961 tcgtctcgcc ggaaccggcg aatgaaactt ctgcgaacca tggttccgac cgggtcctta
     4021 cttgcggaga acgggcgcat agaaattctg cgcgatgtct ttgtaggccg cgacaagttg
     4081 ctcatccgtt gcgacgttgt agcaattctg gcggtgcttg cagcgaagca tggcgctggg
     4141 gttgtcttcc cgggccgtgg cgtcgctgta ggcgccgctg gcgcagcggg cgaagacgtt
     4201 gttgatgatg tcgccgccca catccgactc ctgcacgtcg aacaggatgg tgaaaatctg
     4261 gacgttgttc tctttcagga atttgcagat ccggatttgc tcatcgttgt atggcttgta
     4321 gttgtagaca tactggttgt agaaatttcc gggtcgccag ttcgtgaagc aacacccgat
     4381 gttcgatccg tcagacatca ggatcagcac tttcttgatg tcgcgggtgt tgtagggctt
     4441 cacctcttcg gcgcccggga agtaggtcct ctgcgttggg tcgagcatga gaccggccgt
     4501 atgcgtcggc aggtcggtcc tggtccaggc gacggcctcc ggcgtcagcg tcatgcggtc
     4561 cagggcttgc tgaagcaggg tggtgttgga ggtcatggac tggatggcgc cgagcggctc
     4621 gaagccattg aagtccgcgt agtagccctg tgcctcggat tgcccgacgc tgggcggaat
     4681 catggtcgga ttggcgaatt tgaccttggc gttgtagggc acaagcccga ccttgaagct
     4741 cgcggtcgcc tggttggcct tgacgatgtc gatgaaggcc ttgacgccgg tcttcatggc
     4801 gttgaaatgc cggcagcccg gatcgggcgt cagttccagc aacgtgtcgc cgggaacctt
     4861 gcggtttgtg ttcggcgtcc ggcccttgtt tgagcacatc gttcctgagg ccggcagccc
     4921 catcacgact tccaggttgt tgacctcgcg cttggcttgc gcccggtgct gaaccccgag
     4981 cttgtgaatc ccgaccacct gaccgaggat ggtctgcacc tccgcctcga tgtccacctc
     5041 gacgatgacc ggaccgttcc cggtcgaact gcgcactttc acgccaccgg gcttgatcga
     5101 gacagcttgc ctgtagctct ccggaacctg ggcgttgaag aaccgctcgg ccaccgcgac
     5161 gcgatcctca ccatcgaccg aggctgcggc gagtgccgcg tggtccgccg cgttggcgac
     5221 gattgtcttg gcgcgcagga accgcgtcgt atccacggca agtcccgcga atccgatcag
     5281 ggctggcagg gcgagtgcca cgatgatggc gcttgagcct tgccggtcgg ctggcaggcg
     5341 agcgccagcc gacagcaggc gcgagagcag gcgcttatgg ccggcaggac ggggatcaat
     5401 cgacaccgac gctatccttc acgatcagtc cgttggcgat gaaggtcccg ttcccccgcg
     5461 tacgcaggtt gtaggtcctg tacccgtcag tttccggcac ggcttcgatg gtttcgaccc
     5521 gaacgctgcc gttgagggtg accagtgtgt cgcccaccac gagtttcggc acgtcctgca
     5581 atccgtactt gaaggcgcca tcctcgaagt cgatggcctt ccagcccgtc gtggtcatga
     5641 cgggatgatc cgccgtgagc gtgatggccc cgttgatgcg atggagaatc acgcgcgacc
     5701 agtcgagcga aaccagttcc agcacggtat tcgtgcgccc gaggccggcg acgacatcac
     5761 cggcgcgaat gtcctcgatc accttctcgg acccgtcggc catctggatg cgcgttccgg
     5821 cggccaggca gtcgccgcag gtcgagcggg tctggaccat cgcgccgagg cagatattgg
     5881 aagattgctt gtcgtcgcag gtcagcttcg actcgagtcc ctgaccggcc ggaatgacct
     5941 tccacgggct cggaagctgg cgcgtctgca tgaaggcggc aacccggctc ggcatctgaa
     6001 cgccgttgaa cgtcgtcgaa agcttggcgg cggtatcata gaacgagcgg tcgattgtat
     6061 tgaccgcgat gccgagcgcc gtcgccgcat ccgtccggca cgtgatctcg caaagggcat
     6121 agtccgccag cgccttggtg ttgggagacg ttactccggt gttgtgcttg ggagcggcat
     6181 cgagcagcgc cttctggaga ggaccgcact tcttggcgag acaggcgttc aggtccttgg
     6241 ccgcattcgc acgccgctcc ttctccttgt tgtcggaagt ctgcaagggc ttgtaatgat
     6301 tgcagaactc ggccttctcc ggcccggctc ggaaatagct ggggcagggg ccgccgccat
     6361 tgtggatcca ttcaaccgtg cactgatacg ggcgatcgca gcacatttcg gccatgaacg
     6421 acggcattct cgcttccgac gtccactcgt ccattgtcgt cgggcagaag tccttgggcg
     6481 ggcagtgaca ggccggaatg ttttgcggat tgctccagcg catcattttg cccggcactg
     6541 gcaggcagga aggcgtcacg gccttccatg cgagctgggc ctgtgcagtc gcgaccagtc
     6601 cgagcagggc aaacgttgct gcgatcgggc gcaaacgcct caaggagtct cggcctgttg
     6661 ctcgcacttg cgttagcccc gggttagatc atcaatatcc tatgaaatct gtcgcatgcc
     6721 ggctctttcg atcacgttaa ttgcgaacga aaaagcgctg tccggaggca aaaagcggat
     6781 gttactgctc catttcgggt tgaaattcag cgcaaggcat cgcacgctcg gccgttcctt
     6841 tgcgacggag ttcgatgtct actgaagtcc tgcggctgat agtctttgcc gcgatcctga
     6901 cgagcctcat ggtggtcgag catttcgctc cctggcgtgg gcaggctcag cgtttgcggc
     6961 ggtggccgtc caacctcggc gtgctgttga tcgattctgc gctggtgcgc ctggttttcc
     7021 cggtcaccgc tgccgggttt gcggaactgt gcgcgctgaa gggctggggg ctgttcaacc
     7081 tgctggccgt tcccggctgg atcgccttcg tcctgtcagc ggttgccctc gacctggcga
     7141 tttatttgca gcaccgcgtt ttccatcacg tgccggcact ctggcgcctg catcgcatgc
     7201 accatgccga caccatgctg gacgccacgt cggggacgcg gttccatcct ctggaaatcc
     7261 tgttgtcgat ggccatcaag ctggccgcca tcgcagtgct gggcgcgccc gccgcagcgg
     7321 tgatcctgtt cgaaatcctg ctcaacgcaa cagcgatgtt caaccatgcg aacatcgttc
     7381 ttccgacccg agcagatcgc gttctacgat ggtttctggt cacgccggac atgcaccggg
     7441 tgcaccactc cagcgagcgc atcgaaacgg acagcaactt cgggttcagc ctgccctggt
     7501 gggactatct gttcaagacc tacaacgagc ggcccgaaaa gggcctcgac ggcatggaaa
     7561 tcggcctgga ggtctttcgt gatccgggcg agcagcggct cgatcgcatg ctgacgcaac
     7621 ccttccgtga cccgtaggtc ctgaccgggc ttttcacgat ctttcggaaa aatccgccag
     7681 aaaacaggcg attctcaaga tttttaaact tgttgtgcaa cgtgacttga gatcgtgggg
     7741 cgaaccggcg aaaagccgcc gacgatcacg gaatcgcgag gggcagcgac atgtgtggca
     7801 ttgtgggcat tctgggcgtc ggtcctgtcg cgccccaggt ggtcgattcc ctgaagcggc
     7861 tggagtaccg gggttacgat tccgccggca tcgccaccct tgagaacggc cacctcaccc
     7921 gccggcgcgc ggaaggcaag ctgcgcaacc tgcaagcgct gctgacgctg gagccgctgg
     7981 ccggccatat cggcatcggt cacacccgct gggcgacgca cggtcggccg agcgagtaca
     8041 acgcccatcc ccacgcaacc gaccgcgttg cggtcgtgca caacggcatc atcgagaatt
     8101 tccgggaatt gcggcaggaa ttgacgggcg agggcgtcaa tttctcctcc gacaccgata
     8161 cggaggtcgt cgcccatctg gtgacgcggg aactgcgcaa aggagcaagc ccgcgcgacg
     8221 cagtcgcctt cgcgctcaag cggctgcacg gcgccttcgc gctggccttc ctgttcgagg
     8281 gcgaggaaaa tctcctgatc ggcgcccgtc agggggctcc cctggctgtc ggctacggcg
     8341 acggcgagat gttccttggg tcggacgcgc tcgcgctggc accgttcacg gacgtcattt
     8401 catacctcga cgatggtgac tgggtcgagt tgacgcgcaa ttccgcagtt tttcacgacg
     8461 ccagcgacgc catcgtcgat cggccgcgcc agaagaccca ggccggggca tttctggtgg
     8521 acaagggcaa tcatcgccac ttcatggcca aggagatcca cgaacagccg gaagtcatcg
     8581 gtcgcacgct ggcgcattac ctcgacatgg ccgccggacg ggtcaacctg ccgttcgcgc
     8641 tgccgttcga tttccggacg ttgcgtaaag tctcgatctc ggcctgtggg acggcctttc
     8701 ttgccggctt gacggcgaaa tactggttcg agcgcatcgc gcgccttccg gtcgagatcg
     8761 atatcgcctc cgagttccgc tatcgcgaaa tgccgctcga caaaggcgaa ctcgcaatct
     8821 tcatttcgca gtcgggggaa acggccgaca cgctggcctc gctgcgttac gccaaggcct
     8881 gcggccagca cattctgtcc gtggtcaacg tgccgacatc gacgatcgcg cgcgagagcg
     8941 acgtggtcgc gctgacgctg gcggggccgg aaatcggcgt cgcctcgacc aaggccttca
     9001 cctgccaact gtccgtgctg gcggccctcg ccgtcgcggc aggcgcagcc cggggaacaa
     9061 ttgatgcgac gcgcgagcac aagctggtct ccgacctcat gctgatccct ggccttgtcg
     9121 ccgacagcat caagcgcgag gcgcaggtgg aacagattgc gcgcgagctg accaaggcca
     9181 aggatgttct ctacctcggt cgcggcacat cctatcctct ggccctcgaa ggcgcgctga
     9241 aactgaagga aatcagctac atccatgcgg aaggctatgc ggcgggtgaa ctcaagcacg
     9301 gtccgatcgc gctgatcgag gaaacgctgc ccgtgatcgt cctcgctccg cgcgaccagt
     9361 ggttcgacaa gaccgtgtcg aacgtgcagg aggtcgccgc gcgcggcgga cagttgctgc
     9421 tgatcgggcc gaagggcatc gccgatcagg ccggactgac atcggccgag agcatcgtca
     9481 tgccggacat ggagccggat ttttccgcca tcgtctatgc cataccggtg caattgctgg
     9541 cctattacac cgctgtgcat atgggtaagg acgtcgacca gccgcgcaac ctggcgaagt
     9601 cggtgacggt cgaatagcaa atcgccgcgg ccgagaaccc gggacaccgc taccctcggg
     9661 ggcacgccgc ggttcgttgc gaaatcatgc cgcctcggtc gagtttacat cggtgcaacc
     9721 atccccgctt cccttaggtt aaggttgcgg cgcattcatc ctttcgcgtt aacggctttc
     9781 acgcggcgtt catgcgaggt ttcctgccga cgagagaggc ggacacagcc tcccgcaggg
     9841 acaggggtca gaccgatgac aggagccgtc atgcgcgccg tgcgccagac aagcccgcaa
     9901 ttgcaggtcg tgccgaaggc cgaaccgcag ttcgatgtga cattcaagcc ggtgatgacc
     9961 cggcgcgagg cagaaatcct gttcgagatc gactcggtac agaatggtga caacccggcc
    10021 tggaccgcgt tcttcgtgga aagtcttgtc gaattcgtcg tctatggctc gcgtccgaca
    10081 ggccaggtca gcgacacgga tgcccagtgg atcgtctctt gcgtcggaac ggaagcctcc
    10141 gcgagcgttc cggctctgct gcgggcgatc atcctgcagg ccgagaatgt tccggggtgc
    10201 ctgatccacc tcgccatgcg gtgcggagca atgcgcggac cgtctgccgt tctcatctga
    10261 cgaacggatc atttacgcgc cccacgcgct cgcctaaaac ctcgccccaa tcagatcgaa
    10321 acggtcgggg tgagagcatg ttcggaaaga tactgatcgc caaccgtggc gaaattgcct
    10381 gtcgcgtcat gaaaaccgcg aaccgcatgg gcataaagac ggttgcggtc tattccgacg
    10441 cagacaggga tgcgctccat gtcgcgatgg ccgacgaggc ggtgcatatc ggcccgccgg
    10501 ccgcagccga atcctacctt gtcatcgaga agatcattgc ggcctgcaag cagaccggcg
    10561 cggaagcggt gcatcccggc tacggctttc tctccgagcg cgaggctttc gccgaagcgc
    10621 tgaaggcagc gaacattacc ttcatcgggc cgaaccccag ggccatcgcg gccatgggcg
    10681 acaagatcga atcgaagaaa ttcgccaacg ccgcaaaagt ctcgaccgta cccggctatc
    10741 tcggcatcat cgacgggccg gagcaggcgg tgacgatcgc cgaggagatc ggttatcccg
    10801 tgatgatcaa ggcctccgcc ggcggcggcg gcaagggcat gcgcatcgcc cattcggcgc
    10861 atgaggtggc tgacggcttc gcccgcgcca agtccgaggc gaaggcctcc ttcggtgacg
    10921 accgcgtgtt cgtggagaag ttcatcaccg atccgcgcca tatcgagatc cagctcatcg
    10981 gcgacaagca cggcaacgtc gtctacctgc acgagcgcga gtgctcgatc cagcgccgca
    11041 accagaaggt tatcgaggag gcaccctcgc cgctgctcga cgccgccacc cgcaagaaga
    11101 tgggcgagca ggccgtcgct ctggcgaaag ccgtcgacta cgattccgcc ggcacggtgg
    11161 agttcgtcgc cgggcaggac cggtcgttct tcttccttga aatgaatacc aggcttcagg
    11221 tggagcatcc ggtgacggag ttgatcaccg gcatcgacct cgtggaggag atgatccgcg
    11281 tcgcggcggg cgagaagctg cgcttcacgc aatccgacat caagcttgac ggctgggcgg
    11341 tggaaagccg cgtctatgcc gaggacccgg cccgcaactt cctgccgtcg accgggcggc
    11401 ttgtcaccta tcgcccgccg acggaaggct tctttgacgg cgtcaaggtg cgcaacgaca
    11461 ccggcgtttt cgagggcggc gaaatttcga tctactacga tccgatgatc gccaagttgg
    11521 tgacccatgc gccgactcgc cttggcgcca tcgaggcgca agctgaggcg ctcgacgcct
    11581 tcgccatcga cggcatccgc cacaacatcc ccttcctctc cacgctgatg cagcatccgc
    11641 gctggcgctc cggtgcgctc tccaccggct tcatcaagga agaattcccc gagggtttcg
    11701 ttgcgccgca tccggaaggc gagattcgcg accgcatggc ggcggtggcc gccgtgctcg
    11761 atcaccagtc caaccatcgc cggcggcaga tttccggcca gatcgacggc gcgcggaccg
    11821 accgcatcca gcgcgtgcgt gcggtcatgc tgggcgacga gcgcgtcaac ctcgaaacgg
    11881 agatgacgaa ttccggcttc accgtgactt tcctcgacgg caataagcgg cgcagtgtga
    11941 aagttgttcc ccaatcgctg ccgggtgaac tcgtctggcg cggaactttc gacaagacgc
    12001 cagtcgcggt gcaggtgcgg gccgtcaaca acggctaccg gctgtcgcat ggcggcgcgg
    12061 ccgccgtcgt ctatgtctat acgaaacggg aagccgatat cttcgccctg acgccgaaga
    12121 aacagaaggc cgacacctcc aagacgttgc tctgccctat gccgggcctg gtcaaacaga
    12181 tcgcggtgaa ggagggccag gaggtcaagg ctggcgacat cctcgccgtg gtcgaggcca
    12241 tgaagatgga gaacgtgctg cgggccgaaa aggatgtgac cgtcaagtct gtcaaggcga
    12301 aggaaggcga cagtcttgcc gtcgatgcgg tgatcatgga gtttgcatga ccctggcccg
    12361 atccggcttc gcgccgggca tccacgagat cgaggcctat ggccggggcg gcttccgctt
    12421 tggcggcgtc tcgcatatcg gctcgatcct ggcgacgcca tccgggattc acgcttggtc
    12481 ccccaggtcg gcgcgtgaaa tcacaccgga ggtgctggaa gcgcttgcgg cggacgccgc
    12541 gcggatagac ctgctgtttc tgggcacggg ccgcgatctc gtgccgatgt ctgcgcctgt
    12601 cagggagcac ttggcatcga ttggcctgcg gtttgaggcc ttgccaaccg ccgccgcggc
    12661 gcgcacctat aacgtgctgg tgcgcgaggg gcggcgggca gcggccgggt tgatcgcggt
    12721 cgactgaact tttgcttagt ccggactttt caagccggaa ttttgcgcca cataagcgtc
    12781 aaccgacgga tggctatgaa tttccaggcg cccaaatcca tcatgcttga aaaggcctac
    12841 gcagcctgct gcgagcatgt gcggactctg gatgccgacc gttatctcgc gactctcttt
    12901 gctccggcgg agcgccagcc gcacatcttc gcgctctacg ccttcaatgc cgagatcgcc
    12961 cgcgtgcgcg gcctggtcag cgatccgctg cctggcgaaa tgcgcttcca atggtggcgc
    13021 gacctgcttc atggcgaggc acagggggat gcggcccgca atcccatcgc cattgctctg
    13081 gtcgccacga ttcagacctg caagcttccc gtgaagccat tcacggacct gctcgaagcc
    13141 cgaaccttcg acctctacga tgatccgatg ccgacctggc tcgattgcga ggggtactgt
    13201 ggcgaaacct cttcggcgct gatccgtctg accagccttg cgctgggcgg cggggcggac
    13261 aatgacgcag ccatcgcggc cggccatgcc ggagtcgcct tcgcgctcac cgggctcctg
    13321 cgtgcattcc cctggacctg ccggcgcggc cagctcttcc tgccgaagga ggtgttcgcg
    13381 gcgcacggcg tggatttggc ggacctgccg caaggcaagg attcgtcggg attgcgcgcg
    13441 gcgctcgggg aggtgcgcga gcgggccatg gatcatctgc gcaagacacg ggagctcatt
    13501 ggctcggtct cgccccgcat agctcccgcc ttcttcccct gttgcctggt cgaaccctat
    13561 ctgcggcaga tggaagcgaa ggactacgat cccttccgca cccgcatcga cgtctccagg
    13621 cttctcaagc tcttcacgct ctggcggcaa tcacggaggt ctagggggta gatgcgctcc
    13681 agcgttagcg ttcgccaggc gcgacatgcg cgtcgcacca tcctgccaaa tccgccatcg
    13741 cccgcgccgt cagcgctttc tttttcgcca atgccttgtc cttgcccctc aggcgcttgc
    13801 catccggcga acgcggcgcg gagtcgagcg gcgggaatag gccaaagttg acgttcatcg
    13861 gctggaacga gcgcggcccg gcgtcgatcg tatcgatatg acctccggtg atgtggttga
    13921 tcagcgcgcc gatcgcggtt gtcgccggag gcggtgccat cacccgtcca tggcgttcgg
    13981 ctgcggccag ccgccccgct atcagtccaa cggccgcgct ttccacgtag ccttcgcagc
    14041 ccgtgatctg cccggcgaag cgcaggcgcg gatcggccct gagacgcaac cggttgtcga
    14101 gcagacgcgg cgaattgaga tacgtgttac ggtgaatgcc gccgagccgg gcgaagacgg
    14161 cgccctcaag ccctggaatc agccggaaga tgtcggactg cgcgccgtac ttgagcttgg
    14221 tctggaaacc gaccatgttg aacagcgtgc cgagcgcgtt gtcctggcgc aactgcacga
    14281 cggcatgggg cttgaccgtc gggttatgcg ggttggtgag gccgaccggt ttcatcggtc
    14341 catggcgaag cgtctcgcgg ccgcgctcgg ccatgacctc gatgggcagg cagccgtcga
    14401 agtagggcgt gttcttctcg aactcacgga attctgtctt gtccccggcc agcaaggcat
    14461 cgacgaaggc ctcatactgc tcccgatcga gcgggcagtt gatgtagtcc gctccggtgc
    14521 ctcccgggcc ggccttgtcg tagcgcgact ggaaccaggc cttcttcatg tcgactgagt
    14581 cgaaatgcac gatcggcgcg atggcgtcga aaaaggccag cccgctttcg ccggtgaggt
    14641 cacggatgcc ctgagccaga gcgggcgagg tgaggggacc ggtagcgatg atgacgctgt
    14701 cccagtctgc cggcggcagg ccggcgatct ctccgcgatt gatggtgatc aacggatgtg
    14761 cagcaagagc ggcagttacg gcctgcgaaa agccgtcacg atccaccgcg agcgcggcgc
    14821 cggccggcac ctgatgcgca tcggcactgc gcatgatcag cgaatccata cgccgcatct
    14881 cggcgtgcag caggccgacc gcattggtct gccagtcgtc cgagcggaac gaattggagc
    14941 aaaccagttc ggcgaggcct tccgtcttgt gcgcgtcggt cattcgttcc ggacgcatct
    15001 catgcaggac gaccggcacg cccgacacgg cgatctgcca ggcggcctcg ctgccggcga
    15061 ggccgccgcc gatgacatgg atgggactga gggaagtcag tgtcttgctc atgccgcccc
    15121 gttacggcga agccggagca gacgcaacgg ggcagggcag tacagattgg cattgccgtg
    15181 cgcccaaaag caaaaaggcc cgctcgcgcg ggcctgacgg ttgccatatt caacaggcgg
    15241 tcgatgctca gtgcgccgcg ctgcactcgc gtgcgacctg ctcgatgcag gaccggttga
    15301 cgccgatgtc ggcgagttcg cgatcagaca gggcgttcag ttcgcgcatg ctggcacgga
    15361 ccttcagcca ggtctgaatg cgggcgagga tcacggacaa gatcataggt agtctccttc
    15421 acttctgcct ccggtcggtc tgaccgtggg caatctcttg tgcagcgcaa tatgggcact
    15481 cccgtggcga gggtgtctgc cattgatgtg tgccagccat gcaaaaagcg catagaaaaa
    15541 ggccagcgct cacgcgccgg cctgaagtgc agttactcgt ctcaggcggc ttacttcgtg
    15601 ccgtcggaat ccttggccgg gacgcgggtc tcgccgacgc gccagcgctg cggtgccagg
    15661 aagacgcggg tgcgcttctc accttcgaag tcgagcggcg gacggttgtt gtcgatgaac
    15721 ttgccgatgt tgtcctggaa gctctcgcca tcgcgccaca ggccaacgtt gtagaaagtc
    15781 gtgatgccgg gcttgtcgat cgggccgagc cagttgatcc agggccattt ctccttggag
    15841 tctacggtgc tcaggaactc agcaacgaga ccatcgcggt tcgggatggt cgagcgctcg
    15901 gcccagtagt ccaggaactc ctgctcgcga cccttcttga tcttccactc gaccacgacg
    15961 atgcgcatgt tcgagccctg cgcgaccttg gcagcagcgg cgacggcggg agggaccgcg
    16021 gcgaccttcg tcgccttctg gttctgggca ttcgctccgg cgggcttcag tggcagctca
    16081 tccgcaagcg tgatgctgcc gagcgccacg ccaaagccga aggcggttgc aatcgcggtt
    16141 ttcgcgaaga tggatttgat acgcatgctg acaactccca acccggcggt tcatccgccc
    16201 ttcccgcggg aggtgtccat tttcggcaca gtattgcaat gattaacgta acgtatgctt
    16261 aacttaacca agtctgttaa agcaacgcgc tcaagcagcg catccgttac acccgcgcct
    16321 gtttattcgc agcagccaaa gggcgggcag gtgctggcca tgcccgcatc cccggccgca
    16381 gcgggccaag cagccagcac acggatagcg agaatgcggg cggcgcggca aacaggtcgc
    16441 gtcaggacgt ggcacgcggc ctgcgcttca acacgtcgtc gaggaatcgc ccggtatggc
    16501 tggcttcggc ccgggcaatg gcctctggcg gtccttgcgc cacgatcctt ccgccgccat
    16561 cgccaccttc cgggccgagg tcgatgaccc agtcggcggt cttgatgacc tcaaggttgt
    16621 gctcgatcac caccacggtg ttgccctggt ccaccagctc gtgcagcact tccatcagct
    16681 tggccacgtc gtggaagtgc agcccggtgg ttggctcgtc gagaatgtag agcgtccggc
    16741 cggtggcgcg cttagacagt tccttcgaca gcttgacgcg ctgcgcctcg ccgccggaga
    16801 gcgtcgtcgc ctgctggccg acggagatgt acttgaggcc gacgcgggcc agggtctcca
    16861 tcttgtcgcg gatcggcggc accgccttga agtgttccgc cgcctcctcg acgctcatat
    16921 cgagaacgtc cgcgatcgac ttgccacgga acttcacctc gagtgtctcg cgatcatagc
    16981 gcttgccctt gcagacgtcg caggtgacgt agacgtccgg caggaagtgc atctcgatct
    17041 tgatcacgcc gtcgccctgg caggcctcgc agcgcccacc cttgacgttg aaagaaaagc
    17101 ggcccggctg gtagccgcgc gccttggcct ccggcagtcc ggcgaaccag tcgcggatcg
    17161 gcgtgaacgc gccggtatag gtcgccgggt tggaacgcgg cgtccgtccg atgggcgact
    17221 ggtcgatgtc gatgaccttg tcgagcagtt ccaggccttc gacgcggtca tgcggggcag
    17281 ggtgctccag cgcgccattg aggcgccgcg cagcggcctt gaagagcgtc tcgatgatca
    17341 gcgtcgactt gcccccgccg gagacaccgg tgacgcacgt gaacaggccc agcgggatct
    17401 cggccgtcac cgacttcaga ttatgaccgc gagcgccgac gacccgaagc atgcgcgact
    17461 tctgcggaac gcggcgtttc ttcggcatgg gcacggattt cgtgccggac agatactgcc
    17521 ctgtcagcga cttcggatcg gcgatcacct cctcgggcgt gccctgcgag acgatgcggc
    17581 cgccgtggat gcccgcgccc ggcccgacat cgaccacatg gtcggcctgc cggatcgcgt
    17641 cctcgtcatg ctcgaccacg atgacggaat tgccgaggtc gcgcaggcgc ttgagcgtgc
    17701 cgagcagacg ctcgttgtcg cgctggtgca ggccgatcga cggctcgtcc agcacataga
    17761 ggacgcctgt caaccccgag ccgatctgcg acgccaggcg gatgcgctgg ctttccccgc
    17821 cggacagcgt gccggaagac cgcgccagcg tgaggtattc gagaccgaca tcgacgagaa
    17881 agcgcagacg atccctgatc tccttcagaa tacgtccggc gatttcattc tgcttcgccg
    17941 tcaggcgctc gggcagggtg ttgtaccagt cggcggcgtc cttcaccgac aaccgcgtca
    18001 cctccccgac atgccgtccc gcgatcttca cggccagcgc ctccggcttg agccggtgcc
    18061 cttcgcacgt caggcacggc gtctcgccca tgaaccggcc gatctcctcg cgcgcccagt
    18121 cgctttcggt ctccttgaac cggcgttcga ggttgatgat tacgccttcg aaaggcttct
    18181 tcacctcgta ggaacgcagt ccgtcgttgt aggccatgcg gatggtttcg ccgcccgagc
    18241 catagaggat gacgttctgc gcctgtttcg gcagcttttc ccaggggacg gtgagcgaga
    18301 atccgtagtg cttggcgagg gcgtccaggg tctggccgta gtaggggctc gtcgacttcg
    18361 cccagggcgc gattgcgcct ttcttcagcg tcaggctggc atcgggaacg atcaggtctg
    18421 catcgatctt cagttccgag cctataccgc cgcaatgcgg gcaggcgccg aatgggttgt
    18481 tgaaggaaaa caggcgcggc tcgatctctg gaatggtgaa gccggaaacg gggcaggcga
    18541 atttcgagga gaagatctgt cgcctcggcg acccgtcggc cgtctgctcg tccacccatt
    18601 cgatcacggc gatgccgtcc gccagctcca gcgcggtctc gaggctttcc gacaggcgcg
    18661 caccaatgtc gggccgcacc acgatccggt ccaccacgac gtcgatgtcg tgcttgatat
    18721 tcttgtccag cgccggcgcg tcggcgatct cgtgcatcgc gccgtcgatt ttcacccgct
    18781 ggaagccccg cttgagaaac tcggccagtt ccttgcggta ctcgcccttg cggccccgca
    18841 ccgccggcgc cagcaacagc agccgcgtct tctccggcag ggccagcacc cggtccacca
    18901 tctggctgac ggtctggctc tcgataggca atcctgtcgc cggcgagtac ggcacgccgg
    18961 cgcgcgccca aagcaggcgc atgtagtcat agatttcggt caccgtgcca acggtggagc
    19021 gcggattgcg cgaggttgtc ttctgctcga tggagatggc cggcgagagg ccgtcgatct
    19081 gatcgacgtc cggcttctgc atcatttcca ggaactggcg ggcataggcc gagaggctct
    19141 cgacatagcg gcgctggcct tcggcataga tcgtgtcgaa cgcgagcgag gactttcccg
    19201 atccggaaag cccggtgaac accaccagct tgtcgcgcgg gataacgaga tccacgttct
    19261 tgaggttgtg ctcgcgcgcg ccgcgtatgg cgatcacgcg gccatccacg cttccacctg
    19321 cggccttggc gcggtcgaac agggaatcga caggggtctt gtcggcatcg cggcgggcgc
    19381 gggccgatcc ggaggcaggt ggcgtcatgg cggaaccgtg tttccgtcct gtctactgcg
    19441 ggcgcgaccg tcaggcacgc cgagattgag tcttgcacac agcatgatgt cggaataaga
    19501 acaagacaag aacaaaattc cctgtggacc gctcaatttg ccatcggcaa ccctgcgcgc
    19561 caccggctag aatgcccctt taagtgtgga gagaacaatg gctggcagtc tcaacaaggt
    19621 catgctgatc ggcaacctcg gagcggaccc ggaaatccgc cggctgccga gcggcggatc
    19681 ggtggcgaac atgcgcgtcg ccacgtccga aagctggcgc gacaagaaca ccggcgagcg
    19741 gcgcgagaag acggagtggc acaatgtcgt cgttttcaac gacaatctgg tgcgggtcat
    19801 cgagcagtac atcaagaagg gcgccaaggt ttacgtcgaa ggccagctcc agacgcgcaa
    19861 gtaccaggac aagtcggggc aggaccgcta ttccaccgaa gtcgtgctgc agggcttcaa
    19921 tggccagctg accatgctcg acgggcgtgg cgaaggcggt gggcgcgacg gcggcgatga
    19981 tcgcggcggc tcggatttcg gtcgctcctc gcccatggaa cggcggccgg ctccggcagg
    20041 cgggggacgc cgatccgaca tggacgacga catcccgttc tgacggtccg gacgctgtgg
    20101 cgtgcgctgc gcctgatcga tttctatttc attgatttgc attgtgtttt ggcgtgtcgc
    20161 cggcatggcg ccggcgacct gaatcgccta taacgcccgc acgcgattcg tggagtttcc
    20221 agacccttgg ccgacgacga caagaagccg gacgatccgt ccgaagtcca tcctgtctcg
    20281 atcaccgatg agatgcggcg ttcgtacctc gattacgcca tgagcgtgat cgtgtcgcgc
    20341 gcgctgcccg acgcgcgtga cggcctcaag cccgtgcatc ggcgcatcct gtattcgatg
    20401 cacgagaacg gctacacgcc ggagaaggcc tacaagaagt cggcccgcgt caccggcgac
    20461 acgatgggta aataccatcc gcacggcaac ctggcgatct acgatgcgct cgtgcgcatg
    20521 gcgcaggaat tctccatgcg cctgccgctc atcgacgggc aaggcaactt cggttcggtc
    20581 gacggcgacc cccctgcggc agaacgctac accgaagtgc gtctggagaa ggtggcgctc
    20641 gcgctacttg acgatcttgg cgaggatacg gtcgatttcc agtcgaacta taccggcgaa
    20701 ttcgacgagc cgatggtgct gccggcgaag ttcccgaatc tgctggtcaa tggcgccggc
    20761 ggcatcgcgg tcggcatggc gaccaacatt cctcctcaca acctgggtga ggtgatcgac
    20821 gcctgcaacg cgctgatcgc cgatcctgcg cttggcatcg acgaattgat gcggctggtt
    20881 cctgggccgg atttcccgac cggcgcgctg atccttggac gcggcggcat tcgctcggcc
    20941 tataccaccg gccgcggctc gatcatcatg cgcgcccgcg cgtcggtcga ggagatccgc
    21001 aaggatcgcg aggcgatcat cgtcaccgag attccgtacc aggtgaacaa ggcgacgctg
    21061 atcgagcgca tcgccgaact ggtgcgcgag aagaaactcg aaggcatttc ggacctgcgc
    21121 gacgaatccg atcgccaggg catgcgcatc gtcatcgagt tgaagcgcga cgccatgggc
    21181 gatgtcgtgc tgaaccagct ttaccggcat acggcgctcc agacatcctt cggcgccaac
    21241 atgatcgccc tgaacggtgg ccggcgtccg gagacgatga acctcaagga catgctggtc
    21301 gccttcctcg attttcgcga ggaggtcatc ggccggcgca cgcggttccg cctgaacaag
    21361 gcgcgcgacc gcgcccatgt cctcgtgggc ctggccatcg cggtggccaa tatcgacgag
    21421 gtgatcgcgc tgatccgcgg ttcggccgac gcggcgaccg cgaaggccgc gctcatggaa
    21481 cgggcctggc cggcccatga tgtcgcgccg ctgatcgccc ttatcgacga tccgcgccac
    21541 ggcctgaccg ccgaggggac ctaccgcctg tccgatcggc aagcccaggc catccttgac
    21601 cttaggcttc agcgcctgac cgcgctcggt cgcgacgaga tcgccgagga actggagaag
    21661 atcgccgccg aaatccggga gttcctcgat attctggctt cccgcgagcg cattctcggc
    21721 atcgtgcaga ctgaactgaa cgaggtgcgc gccgcctttg ccacgccacg ccgctccgag
    21781 atcattgagg cggacagtga cttcgaggac gaggatctga tcgcacgcga ggacatggtc
    21841 gtcacggtca gccacggcgg ctacatcaag cgcgttccgc tctcgaccta tcgcgcgcag
    21901 cggcggggcg gcaagggccg ctcgggcatg cagacgaagg aagaggattt cgtctcccgc
    21961 ctgttcgtcg cttccaccca tgcgccgatc ctgtttttct cctcgctcgg ccaggtctac
    22021 aagatgaagg tctggcgcct gccggaaggc gccccgcagt cacgtggcaa ggcgctcgtg
    22081 aatctgctgc cgctggaccc tggcgagcgc atcacgacga tcgtgccgct gcccgaggat
    22141 gaagggcagt ggacggcgct ggatgtgatg ttcgccacaa cgaccggctc gatccgccgc
    22201 aacaagctgt cggattgcgt gccgcgcacc agcctcggca aacgcgtgat ggaattcgac
    22261 gggccagagc aaaccatcgt cgacgtcgca ttggcgaccg aagccgatga cgtgctgctc
    22321 accacggctc aggggcagtg catccgtttc gccgtcacgg acctgcgcgt ttttggcggc
    22381 aatggctcca ccggaacctc gacgggcgtg cgcggcatca accttggcgg cgatgatcgg
    22441 gtcatctcga tgtcgatcct caggcatgtc gaggcgactc ccgaggagcg ctcgacctac
    22501 ctcaagcagg cgcggctgac gcgcggcgag gcaggcgagg cggaggctgc ggcggacgat
    22561 gaagtcgccg attcggccgg caatcttccg ccggagcgct acgccgagat gtcggcgctg
    22621 gagcagcacg tgctgacgat ttcggagaac ggctacggca agcgctcgtc atcctacgag
    22681 tatcgcatca caaaccgcgg cggcaaaggc attgtcgcca tggctgtgaa tgagcgcaac
    22741 ggcgctttgg tggcttcctt cccggtggag aacgccgacc agatcatgct ggtgaccaac
    22801 gg
//
LOCUS       DAOMXN010000017        22360 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_1419_length_22360_cov_12.434028, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000017 DAOMXN010000000
VERSION     DAOMXN010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 22360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22360
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1419_length_22360_cov_12.434028"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..918)
                     /locus_tag="PK812_03050"
     CDS             complement(28..918)
                     /locus_tag="PK812_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRJ68560.1"
                     /translation="MLWAAFTVLASGAQTLRNATQRDLTGRLGALGAAHVRFLFGFPF
                     GLGFLALALLVTGEPLPRMDGRYFAWTAAGGLTQIAATALMLSAMTRKSFVVTTAYTK
                     TEPVQVAIFGLVFLGDRLTPLLALAILIATTGVMILGWPRAAQRGDRKDELQAALSGI
                     AAGGLFALSAICFRGGIHALADGSSWMRASTTLAVGLGLQTLVLLAYLLTRDRGMLAA
                     ILAAWRPSLAAGFLGAAASQFWFLAFALTTAARVRTLALIEVLFAYAISGRLFRERLS
                     LREVAGMGLVVVGVALSLNA"
     gene            1026..1991
                     /locus_tag="PK812_03055"
     CDS             1026..1991
                     /locus_tag="PK812_03055"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HRJ68561.1"
                     /translation="MAVYTEVSVEDLNAFLRPYDLGAIKSFRGIAEGVENSNYVLETE
                     RGRYILTLYEKRVREDDLPFFLGLMEHLQKRGINCPQPVRARDGQILHVLSGRPAAIV
                     TFLDGVSYRRPSVAHCAELGRVLAELHVAGADYAIRRANALSLQGWRPLYAQTGGRGD
                     EVAPGLERLITDELGELETRWPGDLPAGVIHADLFPNNVLFLGDRLTGVIDFYFACND
                     AYAYDLAICLNAWCFESDFAFNLTKGKALIAAYQTIRRLEDREVEALPLLCRGSALRF
                     LLTRLVDYLNVPPGALVRPLDPREYMKRLRFHRGVKDASAYGLAP"
     gene            1991..2458
                     /gene="rnhA"
                     /locus_tag="PK812_03060"
     CDS             1991..2458
                     /gene="rnhA"
                     /locus_tag="PK812_03060"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HRJ68562.1"
                     /translation="MQHVEVWTDGACSGNPGPGGWGAILRYNGTEKELCGGEPETTNN
                     RMELTAAIMALEALKRSCSVDLHTDSQYLRGGITGWIFGWKRNGWKTADKKAVKNVDL
                     WQRLDDLTRRHTVAWHWVKGHADDAMNERADALARAGMAPFKKKAGSTAPAIQ"
     gene            complement(2513..2998)
                     /locus_tag="PK812_03065"
     CDS             complement(2513..2998)
                     /locus_tag="PK812_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012783889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRJ68563.1"
                     /translation="MPIKVGDRLPDVTFVKMTDKGPAPATTGEVFTGKKVVLFGVPGA
                     FTPTCHKNHLPGYLTNVDALKAKGISEIAVTAVNDPFVMKAWSEATGAADKITFLSDG
                     SAIFAKATGLELDMSARGLGIRNQRFSMLVDDGVVKVLNIEETPGTADKTGADAMLKQ
                     L"
     gene            complement(3053..3973)
                     /locus_tag="PK812_03070"
     CDS             complement(3053..3973)
                     /locus_tag="PK812_03070"
                     /inference="COORDINATES: protein motif:HMM:NF022853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HRJ68564.1"
                     /translation="MKLKIASLLFVACVPTLCAAQQQRPGHASEWVRLSGAQVRLVAL
                     PVDPSGPDAWQSAGVEIRLEPGVKTYWRAPGDTGVPPTIDFSPSQGVRELTLDFPAPV
                     GFDDGAGGMAIGYRETQIFPVRFKLEKPAEPTSTGGGFFSRKVEAPPPPVPPKLEMNI
                     EFGICTKNMCVPAQAKAALPLGGGTLEAALGDRIAAANARVPVRQKVGAPGAVGISSV
                     HVRRKAGMAEIEVAARVGNDTGTAGLFIEAKETLVAKRTDAPIGGSVVFRAKTADPAD
                     GKLGEARITLATDKGAVDVLVDLDAAARRP"
     gene            4159..4773
                     /locus_tag="PK812_03075"
     CDS             4159..4773
                     /locus_tag="PK812_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HRJ68565.1"
                     /translation="MTLKQILGKSERRGFLDGQMLVAMPGMQDERFIRTVIYVCAHSS
                     DGAMGLVINKRAPNLGIQDLMVQLKLISQDAGILLPPAVDAIQVLSGGPVEKSRGFVL
                     HSNDFFIESSSLPVDDGIAMTITMDILKAIAAGRGPERALVALGYAGWGPGQLDREMQ
                     ENGWLHCPPDLSLIFDKEHETKYERAMRRIGIDPRMLSSEAGHA"
     gene            complement(4792..7647)
                     /locus_tag="PK812_03080"
     CDS             complement(4792..7647)
                     /locus_tag="PK812_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRJ68566.1"
                     /translation="MAFAIGAFLLSVPVVALEAVRVSPEQDAIDLLPALDTYKSEDDR
                     LTVQTAPGADGIVRRIEVQARESGSRPSWIVFALKNDSDQQIERLLVAPRFRMVDSGL
                     IWPDLGAQRIEAITVSQGQRPEREESGDSDVFSIVLDPGASVTFIAELRTPRLPQLTL
                     WDPEAYKDKVNSHTLYRGIVVGIAALLALFLTVVVTVRGSLIFPAAAALAWAVLVYLG
                     IDFGLFGKFLNVRPETERLYRAGAETVLAGTLVVFLFAYLNLNRWHVRYSQLTAIWMI
                     FLIALLALAIVNAPVAAGVARISIAVVAAVGFALIVYLSFHGYDRAVMLIPTWFLLLV
                     WVGAASLVIGGQLATEFAAPALLGGLVLIVMLIGFTVMQNAFSTGIISRSAVTDAERK
                     ALALTGADDLIFDWDVLEDHVYVSPEFELHFGLKRGTLDGPASSWVDIIYPADRDRYR
                     TMLETVLEQRRGRINQDFRLRSSDGQLYWFRLRARPVVDNNGEVVRVIGTLADVTESR
                     TSEERLLRDAVHDNLTGLANRKLFMERLEAALLFSRTDGAIRPTVFCIDLDRFKQVND
                     SLGPTAADTILLTVARRLTRLLKPHDMLARIGGDGFGLILVSENDRQRILALADLMRR
                     AISAVMIYDGREIVVPATIGVVLFDDQIAPDRDLLLKNAELAVAHGKRAGGDRVEIYR
                     SDMRSDRMDKLAIGSELRRAIERSELRVLFQPIVRLEDRTIAGFEALVRWDHPRLGRL
                     TPGEFIPIAEETGLIHELGLFVMERSARELSLWQQALVVDPPIFASVNISSRQLLRHD
                     LLQDVKSILLKYPSAPGSLKLELTESLIMENPEYAAQMMLRLRELGAGLALDDFGTGY
                     SSLSYLQRFTFDTLKIDQSFVRHTGSGARPVILRSIIALAHDLGMQVVAEGAETEADT
                     VELSQLGCEYAQGFAFGQPMSIEQARRLIGVASNEAA"
     gene            complement(7769..8407)
                     /locus_tag="PK812_03085"
     CDS             complement(7769..8407)
                     /locus_tag="PK812_03085"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010658412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRJ68567.1"
                     /translation="MIAGGRFQPLRLLGVEPAGPRWPSLVVAGGDLVLRPPESADYGA
                     WRELRESSRSFLEPWEPLWPADDLTRPAFRRRTRRYEAEIQADEAYPFFIFASGTGLL
                     LGGLTLGNVRRGAAQSATAGYWMGQAYAGRGIMSQALGLACRFGFQRLGLERIEAACL
                     PGNQASIRVLEKAGFRREGFARSFLNIAGKRQDHFLFALLATDQPASLGTST"
     gene            complement(8404..9804)
                     /gene="thrC"
                     /locus_tag="PK812_03090"
     CDS             complement(8404..9804)
                     /gene="thrC"
                     /locus_tag="PK812_03090"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRJ68568.1"
                     /translation="MRYISTRGQAPVLNFADALLAGLARDGGLYVPETWPRFSPQTIA
                     GLAGQRFDQVAVAVLKPFVGGAIPDTVLERAAHDAYATFGHRAVTPLAQIAPNTFVLE
                     LFHGPTLAFKDVAMQLVARLMDHVLAERSQRATIVCATSGDTGGAAAEAFRGRARTDM
                     VILFPRGRVSGVQRRMMTTPGEANIHPLEIDGTFDDCQAIVKALFNHHAFRDRSRLAG
                     VNSINWGRIVAQVTYYFVAAVALGAPHRKVSFCVPTGNFGDIFAGYVAKRMGLPIDRL
                     IIGTNVNDILARTLDTGRYEVRSVAATMSPSMDIQVSSNFERLLFEEGARDASAIRAM
                     MGSLAQSRSFALPEATLTAIRDGFDAASADEGEVADTIRDTLSGSGYLLDPHTAVAIR
                     AAAKVGGEPGTPMIVLSTAHPAKFPDAVQSISGVEPVLPPRLARLMSDPERMTPLPAQ
                     AQAVETYIDRFLALTA"
     gene            complement(9835..10557)
                     /locus_tag="PK812_03095"
     CDS             complement(9835..10557)
                     /locus_tag="PK812_03095"
                     /inference="COORDINATES: protein motif:HMM:NF014193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HRJ68569.1"
                     /translation="MTRKLVYPAAFTFVSLGILLGLGFWQWQRRSEKAELIQTIQTRI
                     GESPAPLAVLGRLETLGQPTFAYRPVRATGRFDHGQEAHVFFALAKPKNGIGGPGYLI
                     VTPFHLTNGGTILVNRGFVPQDRKAAASREGGQVAGESEITGLIRLPERRGMFANPDD
                     PARNIFYVRDPLTIAASRGLGPVAPVIIDLKTPVPAGGLPLPDVTLVDIPNNHLQYAL
                     TWWSLAAVLGIIFLLWSRQAGR"
     gene            complement(10554..10928)
                     /locus_tag="PK812_03100"
     CDS             complement(10554..10928)
                     /locus_tag="PK812_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HRJ68570.1"
                     /translation="MDERFSTAADPVTAGLSGRCPRCGEGRLFEGFVNLAPACRNCGL
                     SYKFADAGDGPAVFVILIAGFVIIGLSLWVEIHYEPPVWVHLAVFLPLTLIVCLGMLR
                     PLKGLLVGLQYRNKAELGRLDP"
     gene            complement(10995..11864)
                     /locus_tag="PK812_03105"
     CDS             complement(10995..11864)
                     /locus_tag="PK812_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HRJ68571.1"
                     /translation="MAAAHGKTHDYHLVDPSPWPFVGSVSAFVMAVGAIAWMKAMPIA
                     GLKMGSFLFFAGFIGVLYTMFAWWSDVIKEAHGGDHTRVVQIHHRYGMILFIASEVMF
                     FVAWFWAYFDVAFYAGESIQYQRTELTGGHWPPKGIPLFNPWELPLFNTLILLTSGTT
                     VTWAHHAMLHGDREGLKKGLWLTVLLGALFTCVQAYEYGHAGFKFSGHIYGATFFMAT
                     GFHGFHVLVGTIFLIVCLMRAYAGHFTPQRHLGFEFAAWYWHFVDVVWLFLFAVIYVW
                     GSEFGRAATVMAH"
     gene            complement(11910..12518)
                     /locus_tag="PK812_03110"
     CDS             complement(11910..12518)
                     /locus_tag="PK812_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006935705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HRJ68572.1"
                     /translation="MNAAPYLTSARLSARNRRTGWIVFGVVMGMLGLSFAAVPLYDLF
                     CRTTGFGGTPMVASKPADRLGTRIYTVRFDAGVNPALPWRFEAEASSIQVRPGEVKTV
                     TYKVRNISASETVGIASFNVLPEITGGYFNKLQCFCFTDVTLKPGETREESVVFFLDP
                     DIENTPGLSHVDTITLSYTFFPSKTPTKPIAANEQTGGSLAR"
     gene            complement(12515..12685)
                     /locus_tag="PK812_03115"
     CDS             complement(12515..12685)
                     /locus_tag="PK812_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68573.1"
                     /translation="MAEPGKQPEGIVLTEEQKRKQRRRSVALALCIGGLVVLFYLITV
                     VKLGPGILVRKL"
     gene            complement(12691..13620)
                     /locus_tag="PK812_03120"
     CDS             complement(12691..13620)
                     /locus_tag="PK812_03120"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HRJ68574.1"
                     /translation="MTDAGLTRPSAALLSEAGPEDFFALLKPRVMSLVIFTALVGLVL
                     APGHIHPVLGAISIFAIAVGAGAAGALNMWYDADIDRVMGRTANRPVPAGRVSESEAL
                     SFGLILSVLSVGVLGLASNWFAAGLLAFTIFFYAVVYTMWLKRWTAQNIVIGGAAGAF
                     PPMVGWAAVTASATIESFLLFLIIFIWTPPHFWALALVKSEDYARAGVPMLPVVAGEA
                     NTRLQILLYTALLAPTGLAPYAVGLAGPAYALVATLGGAGMLWLAVGVYRRREGKEAT
                     KACWRLFGFSILYLFALFAALLVERLFAMARFG"
     gene            complement(13678..15294)
                     /gene="ctaD"
                     /locus_tag="PK812_03125"
     CDS             complement(13678..15294)
                     /gene="ctaD"
                     /locus_tag="PK812_03125"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HRJ68575.1"
                     /translation="MASSAAHAHDHHDHPTGWRRYVLSTNHKDIGTMYLIFAIVAGLV
                     GGFLSIMMRLELQEPGMQYFASAHTFNVFVTGHGLIMIFFMVMPAMIGGYANWFVPLM
                     IGAPDMAFPRMNNISFWLLPSALALLVISMFVEGPPGANGVGGGWTIYPPLSTSGQPG
                     PAMDFAILSLHIAGASSILGAINFIVTIFNMRAPGMTLHRMPLFVWSILITAFLLVLS
                     LPVLAGGITMLLTDRNFGTTFFAPEGGGDPILFQHLFWFFGHPEVYILILPAFGIISQ
                     IVSTFSRKPVFGYLGMAYAMVAIGFVGFIVWAHHMYTTGISLGAQQYFVAATMVIAVP
                     TGVKIFSWIATMWGGSLRFTAPMVWAIGFIFLFTVGGVTGVVLANAGADRVLHDTYYV
                     VAHFHYVLSLGAVFAIFAAWYYWFPKITGYVIPDWIGQLHFWIAFIGANVLFFPQHFL
                     GLAGMPRRYVDYPDAFAGWNYISSIGSYIFAFGLLVFFYGCAVALIRKEKAGNNPWGE
                     SANTLEWTLTSPPPFHQFETLPRITGSDGH"
     gene            complement(15329..16213)
                     /gene="coxB"
                     /locus_tag="PK812_03130"
     CDS             complement(15329..16213)
                     /gene="coxB"
                     /locus_tag="PK812_03130"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013913397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HRJ68576.1"
                     /translation="MKMAGKFAGAGLAAALVFASASAVLAEDKGMPLPWQKGLQPPAT
                     QIAENIQWFHDGLLLPIITVISIFVLALLIYIVVRFNEKANPVPSKTTHHTMLEVAWT
                     VIPVLILVFIAVPSFKLLREQVVIPKSDMTIKVTGKQWYWTFEYPKDYGGITFDAIML
                     NDEDRAKAIKAGAKAEDMPRLLAVDNIAYVPVNKVVRVQVTAADVIHAFAIQSFGVKI
                     DAVPGRLNETWFKATKEGIYYGQCQELCGKDHAFMPLAIRVVSQDKYDAWLKEAQKKF
                     ATDPSPAMQFAGTPAVQQ"
     gene            16528..17046
                     /locus_tag="PK812_03135"
     CDS             16528..17046
                     /locus_tag="PK812_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HRJ68577.1"
                     /translation="MTLSAFLAVAGLTAAPVSAQGALKATHGDWQLRCDSLPGRSGEQ
                     CILMQSLVDDQNVVEGQKVEINLVVVALKASDPKANNARKPILRVIAPLGVLLPRGLG
                     LKIDTTEIGATGFVRCLANGCVAEVEMDDALIDKFKRGKEATFVIFLTPDEGRGLPLS
                     LDGFEKGFAQLQ"
     gene            17057..17668
                     /locus_tag="PK812_03140"
     CDS             17057..17668
                     /locus_tag="PK812_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68578.1"
                     /translation="MPVSLRSVLVLALGLALAGCQSSGGDQAAASGQPSSWRLAFFGN
                     PQAVPPEKPIESSQRDLNCPSVGVLEGGAAHRIGRQGGSEVSHQASLVDTARECRFAG
                     NSMTLKVGVQGRLLIGAAGRAGTYTVPVRVAVKRGEAVVASRFARVSVTIPANEASAS
                     FVHIEDNIALPVSDNDPAEEYDIYVGFDEGGGPADPRRGTRRR"
     regulatory      17731..17817
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     regulatory      17818..17907
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            17958..19100
                     /gene="gcvT"
                     /locus_tag="PK812_03145"
     CDS             17958..19100
                     /gene="gcvT"
                     /locus_tag="PK812_03145"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006935727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HRJ68579.1"
                     /translation="MSDPAAPAKRTPLYESHVAAGGRMVPFAGYEMPVQYPTGILTEH
                     NWTRENAGLFDVSHMGQAFLLGPDHDTTARALETLCPADFVNLGLGKQRYSQLLASDG
                     GILDDIMVTRSVDPAEDGALMLVVNAACKDADFALIEQRLPANVKLLRAGHRALVALQ
                     GPRAAEVLAKHCPDSADMAFMSARSTRFDNFDCHVSRSGYTGEDGYEISINAKYVATV
                     WDTLCADPLVKPIGLGARDSLRLEAGLCLYGHDIDATTSPVEGALAWSIQKRRREEGG
                     FPGADRIQRELRDGPSRLRVGILPEGRQPAREGAEIHADGRKVGVVTSGGFGPTRNGP
                     CAMGYVEAAFARPGTALTLIVRDKPLPAVVAPMPFVPKRFFQPKAG"
     gene            19122..19499
                     /gene="gcvH"
                     /locus_tag="PK812_03150"
     CDS             19122..19499
                     /gene="gcvH"
                     /locus_tag="PK812_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HRJ68580.1"
                     /translation="MSTIRYTKDHEYIRVEGDIGTVGISDYAQAQLGDVVFVDLPAPG
                     KEVAQGAETAVVESVKAASEIYAPVAGSVVEVNARLADNPATVNEAPLGDGWFLKLRI
                     ADTAQLDALMTEEQYQDYLKTLG"
     gene            19524..20864
                     /gene="gcvPA"
                     /locus_tag="PK812_03155"
     CDS             19524..20864
                     /gene="gcvPA"
                     /locus_tag="PK812_03155"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HRJ68581.1"
                     /translation="MRYLPHTQADRDAMLARIGVGSVDELFASIPAEKRLKSLPDLPR
                     ARTELEVERILSKMAGRNVPASAVPFFVGAGAYKHHVPATVDHLIQRSEFLTSYTPYQ
                     PEIAQGTLQYLFEFQTQVALLTGMDVANASMYDGSTGTAEAVLMAHRVTKRRKAVLSG
                     GLHPHYVDVVRTLSDMAGDDLVVLPPDVTAAEDLAATIDSTVSCVVVQTPDFFGNIRD
                     ISALSAKCKAAGALLIAVFTEAVSLGALQSPGEMGADIVVGEGQSIGNGLNFGGPYVG
                     LFATKQQYVRQMPGRLCGETVDSEGRRGFVLTLSTREQHIRREKATSNICTNSGLCTL
                     AFTIHMSLLGEAGLRRLAAVNHANAVTLADMLSGVPGVEVLTRSFFNEFTVRLKQDAA
                     AVLEKLVERGILGGVPVSRLLPNRGLDDLLVIASSEVNTDGDRAALAGALKEVL"
     gene            20864..>22360
                     /gene="gcvPB"
                     /locus_tag="PK812_03160"
     CDS             20864..>22360
                     /gene="gcvPB"
                     /locus_tag="PK812_03160"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HRJ68582.1"
                     /translation="MLNNQGRPTAAGSATTTGHATFTGNRALMQEEALIFETGRLDAT
                     GVDIEEPAKVANRLGGLERKAAIGLPGLSEPEAMRHYVKLSQKNYGIDTGLFPLGSCT
                     MKHNPRLNEKMARLPGFNDVHPLQPQSTVQGAIDVMAELSRWLMELTGMPAVALSPKA
                     GAHGELAGMMAIKAAIEAKGEGKTRNVVLVPDSAHGTNPATAALIGFSVKSVPADSDG
                     TVRVAEVKKLIGPDVAAIMLTNPNTCGLFEPEIADIAAAMHEAGAYFYCDGANFNAIV
                     GKTRPGDLGVDAMHINLHKTFSTPHGGGGPGSGPVVLSERLAPYAPVPFLHKDGDRIV
                     LVEHADGSQAFGRVTAFHGQMGMYVRALSYMLSHGADGIRQASEDAVLNANYIRAGLS
                     DLMSQPFGDKPCMHEALFDDAWLKDTGVTTLDFAKAMIDEGYHPMTMYFPLVVHGAML
                     IEPTESEGRASLDLFIMALRDLAMAARRGETERFTGAPYHAPRRRLDET"
BASE COUNT         4300 a         7163 c         7262 g         3635 t
ORIGIN      
        1 gcaagttgcg cgatggggac ggacccctta cgcgttcaga ctgagcgcga caccaacgac
       61 caccagcccc atcccggcga cttcccgaag cgacagccgc tcgcggaaca gccggcccga
      121 aatcgcatag gcgaagagaa cctcgatgag tgcgagcgtg cgcacgcggg cagcggtcgt
      181 cagcgcgaag gcgaggaacc agaattgcga tgccgccgcg cccagaaacc cggcggccag
      241 cgagggacgc caggccgcca ggatcgccgc cagcatgccc ctgtcccgcg tcagcagata
      301 ggccaggaga accagcgtct gcagaccgag cccgaccgca agcgtcgtgc tggcccgcat
      361 ccagctgctg ccatcagcga gggcgtgaat gccgccccgg aaacagatgg cggagagcgc
      421 gaacagtccg ccggccgcga tgccggacag ggctgcttga agctcgtcct tcctgtcgcc
      481 gcgctgggcg gcccgtggcc agcccaggat catcacgccc gtcgtggcta tcagtatcgc
      541 cagggccaac agaggcgtga gacgatcccc aaggaagacg agaccaaaga tggcgacctg
      601 caccggctcg gtcttcgtgt aggcggtggt gacaacgaag gatttcctgg tcatcgccga
      661 aagcatcagc gcggtagccg cgatctgcgt caggccgccg gcggcggtcc aggcgaaata
      721 ccggccatcc atgcggggca gcggttctcc ggtgacaagc aaagccagag cgagaaagcc
      781 aaggccaaaa ggaaatccga acaggaaacg cacgtgggct gcgccaagcg ctcccaagcg
      841 gcccgtcaga tcgcgctgcg tggcgttgcg cagggtctgc gcgccggagg caagcacggt
      901 gaaggcggcc cagagcacgc gacgcgatcc tcgatccagc gtttgacgaa ggcaagcgaa
      961 agtcgataac acggcgcgga cgcccgacaa cagcggcgca gaaacacttt tccggaagct
     1021 cagccgtggc ggtctacacc gaagtcagcg tcgaggatct caatgccttc ctccgcccct
     1081 atgacctcgg cgcaatcaaa tcgttccgag gcattgccga aggcgtcgag aactcgaact
     1141 acgtgctcga gaccgagcgc gggcgctaca tcctgacact ttacgaaaag cgcgtccggg
     1201 aagacgatct gcccttcttt ctcggcctga tggaacacct ccagaagcgc ggcatcaact
     1261 gcccgcagcc ggtgcgcgcg cgggacggcc agatcctcca cgttctctct ggacgtccgg
     1321 cagcgatcgt tacattcctc gatggcgtct cgtatcgacg cccgtccgtg gcacattgcg
     1381 cagagctggg gcgcgttctg gccgaactgc atgtggctgg cgcggactat gccatccggc
     1441 gcgccaacgc gctttcgctg caaggctggc gtccgctgta cgcccagacc ggcggtcgcg
     1501 gtgacgaggt tgcccccgga ctggaacggc tgatcaccga cgaacttggc gaactggaaa
     1561 cgcgttggcc cggcgacctg ccggccggcg tcatccatgc cgacctgttt cccaacaacg
     1621 tgctgttcct gggcgaccgg ctgacaggcg tgatcgactt ttatttcgcc tgcaacgatg
     1681 cctatgccta tgacttggcg atctgcctca atgcctggtg tttcgagtcc gacttcgcct
     1741 tcaacctgac caaagggaag gctttgatcg ccgcctatca gacaatccgc cggctggagg
     1801 atcgcgaagt cgaagctctg cctctgctgt gccgcggcag cgcgctgcgg ttcctgctga
     1861 cgcgtctggt cgactatctg aacgtgccgc cgggcgcgct ggtccgtccc ctcgatccgc
     1921 gcgagtacat gaagcgtctt cggttccatc gcggcgtcaa ggacgcctcg gcttacggtc
     1981 tggcgccgtg atgcagcacg tcgaggtctg gacggacggg gcctgttccg gcaaccccgg
     2041 gcccggcggc tggggtgcga tcctgcgcta taacggcaca gagaaggagc tttgcggcgg
     2101 cgagcctgag accaccaaca accggatgga actgaccgca gccatcatgg ctctggaagc
     2161 cctgaagcgc agctgctccg tcgatctgca tacggattcg cagtatctac ggggcggaat
     2221 cacgggatgg atattcggat ggaagaggaa cggctggaaa accgccgaca agaaggcggt
     2281 aaagaacgtc gacctctggc agcggctcga tgatctgacc cgccgtcaca ccgtcgcctg
     2341 gcactgggtg aagggccatg cggacgacgc catgaacgag agggcggatg cgttggcacg
     2401 cgccgggatg gcgccgttca agaaaaaggc cggcagtacc gcgccggcca ttcaatagtc
     2461 ctggggtcgg ggcccattca cagagcgggc ggcgtgccgc ccgcgcgccg cgtcagagct
     2521 gcttgagcat ggcgtcggca ccggtcttgt cggcggtgcc tggcgtctcc tcgatgttca
     2581 ggaccttgac cacgccgtca tcgaccagca tcgaaaatcg ctgattgcgg atgccgaggc
     2641 cgcgcgcgga catgtcgagt tccagcccgg tcgccttggc gaagatggcg ctgccatcgg
     2701 acaggaacgt gatcttgtcg gctgccccgg tggcttccga ccaggccttc atgacgaaag
     2761 gatcgttcac cgcagtcacg gcgatttccg aaatgccctt cgccttcagc gcgtcgacgt
     2821 tcgtcaggta gccgggcaag tggttcttgt ggcaggtcgg cgtgaacgcg cccggcacgc
     2881 caaacagaac gacctttttc ccagtgaaga cctctcccgt ggtcgccggc gcaggcccct
     2941 tgtcggtcat cttcacgaat gtgacgtcgg gaagtcgatc tcccaccttg atcggcatct
     3001 gtctggctcc ttcatgcgca aacggaccgg caccaacgcc ggccgcctga acttagggcc
     3061 ggcgcgcggc cgcgtcgagg tctaccagaa cgtccacggc gcccttgtcc gtcgcgagcg
     3121 taatccgtgc ttcgcccagc ttgccgtcgg cagggtcggc ggtcttggct ctgaacacca
     3181 ccgatcctcc gatcggagca tccgtgcgct tggccaccag cgtttccttg gcttcgatga
     3241 acagaccggc ggtgccggta tcgttgccga cccgagccgc aacctcgatc tccgccatgc
     3301 cagccttgcg gcggacgtgg accgacgaaa tgccgaccgc acccggcgct ccgaccttct
     3361 ggcgcaccgg aacgcgggca ttcgcggcgg cgattcgatc ccccaacgca gcttccagcg
     3421 tgccgccgcc cagcggcaag gcggccttgg cctgtgccgg cacgcacatg ttcttcgtgc
     3481 agattccaaa ctcgatgttc atttcgagct tcgggggaac cggaggcggc ggcgcctcga
     3541 ccttgcgcga gaaaaacccg ccgccggtgc tcgtgggttc ggcgggcttc tccagcttga
     3601 agcggacggg gaatatctgc gtttcccgat agccgatggc catgccgccg gcgccgtcgt
     3661 cgaatcccac cggcgccggg aagtccaggg ttagctcgcg caccccctgc gatggcgaaa
     3721 agtcgatggt cggcggcacg ccggtgtcac ccggcgcgcg ccagtatgtc ttgacccccg
     3781 gctccaggcg gatctcgaca ccggctgatt gccaggcgtc cggtccggac gggtcgaccg
     3841 gcagggcgac gagccggacc tgcgcgccgg acaggcgaac ccactcggat gcgtggccgg
     3901 ggcgttgctg ctgcgccgcg caaagcgtcg gcacgcaggc aacgaacagg agcgatgcaa
     3961 tcttgagttt catgatagcg aaataacacc aattcattgc aggttggttg cctggatcga
     4021 tgaaatcagc tgtcggcggc aaaaactgtt caagattgag ggatcaggac cacaaagtcg
     4081 tgaggcaccg gcgcctgcgt cgacggtgcg gacgaaatca ccccttggca tttcttggcg
     4141 ctgacatagc atatcggcat gacgctgaaa caaatcctcg ggaaaagtga gcgacgcggg
     4201 ttcctcgatg gccagatgct ggtggcgatg cccggcatgc aggacgagcg cttcatccgc
     4261 acagtgatct atgtgtgcgc ccactccagc gatggcgcga tgggactggt aatcaacaag
     4321 cgcgcgccaa acctgggcat ccaggacctt atggtgcaac tcaagctgat cagccaggac
     4381 gccggcatcc tgttgccgcc ggcggtcgac gccatccagg ttctgagcgg cggcccggtc
     4441 gaaaaaagcc ggggattcgt gctgcactcc aacgacttct tcatcgagag ttccagcctg
     4501 ccggtcgatg acggcatcgc catgaccatc accatggata ttctcaaggc gattgccgca
     4561 gggcgcggac cggagcgcgc gctggtggcg ctgggctatg ccgggtgggg accgggacag
     4621 ctagatcgcg agatgcagga gaacggctgg ttgcactgtc caccggacct ctccctgatc
     4681 ttcgacaagg aacacgagac gaagtacgaa agggccatgc ggcgcatcgg catcgacccg
     4741 cgcatgctct cgtccgaggc tggacacgcc tgaaccctgc acgcggtgcc ctcaggcggc
     4801 ttcgttgctg gccacgccga tcagccgccg tgcctgttcg atgctcatgg gttgtccgaa
     4861 ggcgaagccc tgggcgtatt cgcaccccag ctgcgacagc tcgaccgtgt cggcctccgt
     4921 ctccgcgccc tccgccacga cctgcatgcc gagatcatgc gccagcgcta tgatcgaccg
     4981 caggatcacc gggcgtgctc cgctgccagt atgacggacg aaggactggt cgatcttgag
     5041 cgtatcaaag gtgaaccgct ggagatagga cagcgaggaa tagcccgtgc cgaaatcgtc
     5101 gagcgccagc ccagccccga gctcgcgcag ccgcagcatc atctgcgccg cgtactccgg
     5161 gttctccatg atcaggcttt ccgtcagttc cagcttcagg gaaccaggcg ctgaggggta
     5221 cttcagcagg atgctcttca cgtcctgcaa cagatcatgg cgcagcaatt gacggctgga
     5281 gatgttgacc gaggcgaaaa tcggcgggtc caccacgagc gcctgctgcc agagcgacaa
     5341 ttcgcgcgcc gagcgctcca tcacgaacag gccgagttca tgaatcaggc cggtttcctc
     5401 ggcaatcgga atgaactcgc ccggggtgag gcggccaagc cggggatgat cccaacgcac
     5461 cagcgcctcg aatccggcga tcgtgcggtc ttcgagacgc acgatcggct ggaacagaac
     5521 gcgcagttcg gagcgttcga tcgcccgccg caattcgctg ccgatcgcca gcttgtccat
     5581 gcggtccgat cgcatgtcgg agcggtagat ctccacccga tcccccccgg cacgcttgcc
     5641 gtgcgccacg gcgagttccg cgttcttcaa gagcaggtcg cggtccggcg cgatctgatc
     5701 gtcaaacagc accacgccga tggtcgccgg cacgacgatt tcccgcccat cgtagatcat
     5761 caccgcgctg atcgcccgtc gcatcagatc ggccagggca aggatgcgct ggcggtcatt
     5821 ctcagacacg agaatgagcc cgaatccgtc gccgccgatc cgcgccagca tgtcgtgcgg
     5881 cttgagcaac cgcgtcagac ggcgggccac ggtcagcagg atagtgtccg ccgcggtcgg
     5941 gccaagcgag tcgttgacct gcttgaacct gtcgagatcg atgcagaaca cggtcggacg
     6001 gatggcgcca tccgtccggg aaaacagcaa cgcagcttcc agccgctcca tgaaaagctt
     6061 gcggttggcc agacccgtca ggttgtcgtg cacggcatcg cgcagcagac gctcttcaga
     6121 cgtgcgtgat tccgtcacgt cggcgagcgt gccgatcacc cgcacgacct cgccgttgtt
     6181 gtcgacgaca ggcctggcgc gcaggcgaaa ccaatacagt tgcccatcgc tggaccgcag
     6241 acggaaatcc tgattgatgc gcccgcggcg ttgctcaagc actgtttcaa gcatcgtgcg
     6301 gtagcgatca cgatcggccg ggtagatgat gtccacccag gacgaggccg gcccgtccag
     6361 cgtcccgcgc ttgagaccga aatgcagttc gaattccggg ctgacataga catggtcttc
     6421 cagcacgtcc cagtcgaaga tcaaatcgtc cgcgcccgtc agcgccagcg ccttgcgctc
     6481 ggcatccgtt acggcgctgc gcgagatgat tccggtggaa aaggcgttct gcatcacggt
     6541 gaagccgatc agcatgacaa tcagcaccag gccgccgagc agcgctggcg cagcgaactc
     6601 ggtcgccaat tggccgccga tgacaagact ggcggcaccg acccagacaa gcaggagaaa
     6661 ccaggtcggt atgagcatca cggcgcggtc gtagccgtgg aaagacaggt agacgatcag
     6721 cgcgaacccc accgccgcca ccaccgcaat cgagatgcgg gccacgccgg ccgcaaccgg
     6781 ggcattcacg atcgccaggg ccagaagagc gatcaggaaa atcatccaga tcgccgtgag
     6841 ctgactgtag cgcacgtgcc agcggttcag gttcaggtaa gcgaacagga acacgaccag
     6901 cgtgccggcc agcaccgtct cagcgccggc gcgatagagg cgctcggtct cgggcctgac
     6961 gttcaggaac ttgccgaaca gtccgaagtc gatgccgaga tagaccagaa ccgcccaggc
     7021 cagcgccgca gccgccggga agatcagcga accgcgcacc gtgaccacca cggtgaggaa
     7081 cagcgccagc aaagccgcga tacccaccac gatgccgcga tacagcgtgt ggctgttgac
     7141 cttgtccttg taagcctcgg gatcccacag cgtcagctgc ggcagtcgag gcgtgcgcag
     7201 ttccgcgatg aacgtcaccg aggcgccggg atcgagcacg atgctgaaca catcggaatc
     7261 gccgctttct tcccgctcgg ggcgctggcc ctggctgacg gtgatggctt cgatgcgctg
     7321 agcacccaga tcgggccaga tcaggccaga atccaccatg cggaagcggg gcgcgaccag
     7381 caggcgttcg atctgctgat cagaatcgtt cttcagcgcg aagacgatcc acgaggggcg
     7441 agagccgctc tcgcgcgcct gcacttcgat ccggcgcacg atgccgtcgg ctccgggcgc
     7501 ggtctggacg gtgagacgat cgtcttccga cttgtaggtg tccagcgcgg gcaacaggtc
     7561 gatggcgtcc tgctccggcg acactcgcac cgcttcaagc gccacgaccg gaacggacaa
     7621 caggaatgca ccgatggcga aggccatcag gccggcgaga aaccgggcgg tgcgacttct
     7681 gacttgcccc caaatcatgc aaccttggta gccgcgcatg cccgcgcccg caagcgcggc
     7741 tggcagggac gatccacggt cggtcgggtc aggtgcttgt tccaaggctc gccggctggt
     7801 ccgttgccag aagcgcgaac aggaaatggt cctgtcgttt tccggcgatg ttgagaaagc
     7861 tgcgggcaaa gccctcgcgc cggaatccgg ccttttcaag aacacggatc gacgcctggt
     7921 tcccgggcag acaggccgcc tcgatccgtt ccagcccgag ccgctgaaat ccgaaacggc
     7981 aggccagacc gagcgcctgc gacatgatgc ccctgcctgc gtaggcctgg cccatccagt
     8041 aaccggcggt cgccgattgc gcggcacccc ggcgcacgtt gccgagagtg aggcctccca
     8101 gcaacaggcc cgtgccggac gcgaagatga aaaacggata ggcttcatcc gcctggattt
     8161 cggcctcata gcgccgggtg cgcctgcgga aggcgggccg tgtcaggtcg tccgccggcc
     8221 agagcggttc ccacggttcg agaaagctgc ggctgctttc gcgcagctcc cgccaggcgc
     8281 cgtagtctgc cgactccggc gggcgcaaca ccagatcgcc gccagctacg accagacttg
     8341 gccagcgcgg accggcaggc tccaccccca gcagccggag aggctggaac cggccgccag
     8401 caatcatgcg gtcaacgcga gaaagcggtc gatgtaggtc tccaccgcct gcgcctgcgc
     8461 cggcagcggc gtcatgcgct cgggatcgct catcaggcgc gccaggcgag gcggcaggac
     8521 cggttcaacg cccgatatgg actgcaccgc gtcgggaaac ttggcgggat gggctgtgga
     8581 cagcacgatc atgggcgttc caggctctcc accgaccttg gccgccgccc tgatggcaac
     8641 cgccgtatgg ggatcaagga gatacccgct tccggaaagc gtgtcgcgga tggtgtcggc
     8701 cacctcgccc tcgtccgcgc tcgcggcgtc gaatccgtcg cggattgctg tcaaggtcgc
     8761 ctccgggagc gcaaagctgc gggactgcgc gagagacccc atcatggcac ggattgccga
     8821 ggcgtcgcgc gccccttcct cgaacagcag gcgttcgaag ttggacgaga cctggatgtc
     8881 catcgacggg gacattgtgg cggcgaccga acggacctcg tagcgacccg tgtcgagggt
     8941 gcgcgccagg atgtcgttga cgttggtgcc gatgatcagc cggtcgatgg gcaatcccat
     9001 gcgcttggcg acataccccg caaaaatatc gccgaaattg cctgtcggca cacagaaact
     9061 cactttgcgg tgcggcgcgc caagcgccac ggcggcgacg aaataatagg tcacctgcgc
     9121 gacgatgcgg ccccagttga tcgaattgac gcccgcaagc cgggagcgat cgcggaaggc
     9181 atgatggttg aacagcgcct tcacgatggc ctggcagtcg tcgaacgtgc cgtcgatctc
     9241 caggggatgg atgttggctt cgccgggcgt cgtcatcatg cggcgctgca cgcccgacac
     9301 ccgccccctg ggaaacagga tgaccatgtc ggtgcgcgcc cggccgcgaa aggcttcggc
     9361 cgccgcgccg ccggtatcgc ccgacgtggc gcaaacgatg gtggcgcgct ggcttcgctc
     9421 ggcgaggaca tggtccatca gccgcgcgac cagctgcatc gccacgtcct tgaaggcaag
     9481 agtcggcccg tggaataact cgagcacgaa ggtgttcggc gcaatctgcg ccagaggcgt
     9541 caccgccctg tggccgaagg tggcataggc atcatgcgcc gcgcgttcga ggacggtatc
     9601 ggggatggcg ccgccaacga agggcttgag caccgcgacc gcgacctgat cgaagcgctg
     9661 gcccgccagc ccggcgatgg tctgcgggga aaagcgcggc caggtctcgg gaacgtagag
     9721 gcccccatcg cgcgccaatc cggcaagcag ggcatcggca aaattgagga cgggcgcctg
     9781 gccacgggtc gaaatgtaac gcatgcttcc ggatagcacc ggggtgcagc cttgtcagcg
     9841 ccctgcctga cgcgaccaga ggagaaatat gatccccagg acggccgcca gactccacca
     9901 tgtcagagcg tactggaggt gattgttcgg gatgtcgacc agcgtcacgt ccgggagcgg
     9961 aagcccgccg gcaggcactg gcgtcttgag atcgatgatg accggggcaa ccggaccgag
    10021 ccctctggag gcggcgatcg tcagaggatc acgcacatag aatatgttgc gggcgggatc
    10081 gtcgggattg gcgaacattc cccggcgctc gggcagtcgg atcaggccgg tgatttcgga
    10141 ctcgccggcc acctgtccgc cctcgcgcga cgccgcagcc ttgcgatcct gcggaacgaa
    10201 cccgcgattg acgaggattg taccgccgtt cgtcaggtga aacggggtga cgatcagata
    10261 gccaggacca ccgatgccgt tcttcggctt tgccaaggcg aagaaaacat gcgcctcctg
    10321 accgtgatcg aaacggccgg tcgcgcgcac cggacgatag gcaaaggtag gctggcccaa
    10381 ggtctcgagg cgaccgagca cggcaagcgg tgctggcgac tcgccaatcc gtgtctggat
    10441 ggtttggatc agctccgcct tttccgatct gcgttgccac tgccagaaac caaggccgag
    10501 aagaataccc aggctgacaa acgtgaatgc cgccggatag acgagctttc tggtcatggg
    10561 tcgagacggc cgagttccgc cttgttgcgg tactggaggc cgacgagcag ccccttgagc
    10621 ggacgcagca tgccgagaca gacgatcaac gtgagcggaa ggaacacggc gaggtggacc
    10681 cagaccggcg gctcgtagtg gatctcaacc cacagcgaca gcccgatgat cacgaatccc
    10741 gcaataagta tgacgaacac ggcggggccg tcgccggcat cggcgaactt gtaggacagg
    10801 ccgcagttgc ggcatgccgg cgccaaattg acaaagccct cgaacagccg gccttctccg
    10861 cagcgcgggc aacgtccgct caggcccgcc gtcacgggat ccgccgcagt ggagaagcgt
    10921 tcgtccatgg ccctgctccg caaatgccaa gggcggcgtc gccgccgccc tattaagcgc
    10981 tattgctctg acgctcagtg cgccatcacc gttgcggctc gaccgaattc cgacccccag
    11041 acgtagatca cggcgaacag gaacagccag acgacgtcga cgaaatgcca gtaccaagcg
    11101 gcgaattcga agccgagatg ccgctgtggg gtgaaatgcc cggcataggc gcgcatcaga
    11161 cagacaatca ggaagatcgt gccgaccagg acgtggaagc catggaagcc ggtcgccatg
    11221 aagaaggtgg ctccgtaaat atggccggag aacttgaaac cggcgtggcc atattcgtag
    11281 gcctgaacgc aggtgaacag cgcaccgagc agaacggtca gccagaggcc cttcttcaag
    11341 ccttcgcgat caccatgcag catggcgtgg tgcgcccagg tcacggttgt tcccgaggtc
    11401 agcaggatca gcgtgttgaa cagcggcagt tcccacgggt tgaacagcgg gatgcccttg
    11461 ggcggccagt gcccgccggt cagttccgtg cgctggtact gaatgctctc gccggcatag
    11521 aacgccacgt cgaaataggc ccagaaccag gcgacgaaga acatcacttc cgaggcgatg
    11581 aacaggatca tgccgtagcg gtgatgaatt tggaccacgc gggtatgatc gcccccgtgg
    11641 gcctccttga tcacgtccga ccaccaggcg aacatggtgt agagcacgcc gatgaagccg
    11701 gcgaagaaca ggaacgatcc catcttcagg cccgcgatcg gcatcgcctt catccaggcg
    11761 atcgcgccga ccgccatgac gaaggccgac accgatccga cgaacggcca tgggctcgga
    11821 tcaacgaggt gataatcgtg tgtcttaccg tgggcagctg ccatggcccc ttccccttca
    11881 gctagaaaat gatggcgcgt tcgaacagtt catcgggcga ggctcccgcc cgtctgttca
    11941 ttcgcggcga ttggttttgt cggcgtcttg gacgggaaaa acgtgtacga caaggtgatc
    12001 gtgtcgacat gcgacaggcc cggcgtgttc tcgatatccg gatcgagaaa gaacaccacg
    12061 gattcctcac gcgtttcccc gggcttcaac gtcacgtccg tgaagcagaa gcactggagc
    12121 ttgttgaaat agccgccggt gatttccggc agcacgttga agctggcgat cccgaccgtc
    12181 tcggaggcgc tgatgttgcg gaccttgtag gtcactgtct ttacttcgcc gggccgcact
    12241 tggatggacg aggcctcagc ctcgaatcgc caaggcagtg ccgggttgac ccctgcatcg
    12301 aagcgcaccg tatagatccg tgtgcccaga cggtctgccg gcttggacgc gaccatcggc
    12361 gtcccaccga accccgtggt gcggcagaac agatcataga gcggcacggc ggcgaaggac
    12421 aggccgagca tgcccatcac gacaccaaag acgatccagc cagtgcgccg gttgcgcgcg
    12481 gaaagccttg ctgaagtgag atacggagct gcgttcaaag cttcctcaca agtatgccgg
    12541 ggccgagctt cacgacagtg atcaagtaga acagaaccac gagcccgccg atgcacaagg
    12601 caagcgcgac cgaacggcga cgctgtttgc gcttctgttc ctcggtaagg acaatgcctt
    12661 ccggctgttt gcctggttcc gccatcgcca tcatccgaag cgtgccatgg cgaagagccg
    12721 ttcaaccagc aaggcagcga acagggcgaa cagatagagg atcgagaagc cgaacagcct
    12781 ccagcacgcc ttcgtggctt ccttgccctc ccgccggcga tacaccccga cggcgagcca
    12841 cagcatgcca gcaccaccca gcgtcgccac cagggcatag gcggggccgg cgaggccaac
    12901 cgcatagggc gcaaggcccg ttggtgccag cagtgccgta tagagcagga tctgcaagcg
    12961 cgtattcgct tcaccggcca ccacgggtag catcggaacg ccggcgcggg cgtagtcctc
    13021 ggacttcacc agcgcgagcg cccagaagtg cggcggcgtc cagatgaaga tgatgaggaa
    13081 cagcaggaag ctttcgatcg tcgcacttgc ggtcaccgcc gcccagccca ccatgggcgg
    13141 gaacgcaccg gcggcaccgc cgatcacgat gttctgcgcc gtccagcgct tcagccacat
    13201 cgtgtagacc acggcgtaga aaaagatcgt gaaggccagc agcccggcgg cgaaccagtt
    13261 ggaggcaaga ccgagcacgc caacggagag tacagaaaga ataaggccga acgacagcgc
    13321 ctcggactcg ctgacgcgcc cggccggcac gggacggttg gcggtgcgtc ccatcacccg
    13381 gtcgatgtcg gcatcatacc acatgttgag cgcgccggcg gcgcccgctc cgaccgcgat
    13441 ggcgaagatc gagatcgcgc caagcacggg gtggatgtgg ccaggcgcca gcaccagacc
    13501 gaccagcgcg gtgaagatga cgagcgacat cacccgcggc ttcagcagcg cgaagaaatc
    13561 ctccggcccc gcttcggaaa ggagcgctgc ggaaggacgt gtaaggccgg cgtcggtcat
    13621 ctggggaaat gtcgcttcag gaatgggcgg ggcggcgcag gccgccccgc atgggtgtta
    13681 gtgaccgtcg ctgccggtga tgcgcggcag ggtctcgaac tggtggaacg gcggtggaga
    13741 ggtcagcgtc cattccagcg tattggcgct ctcgccccat ggattgttgc cggccttttc
    13801 cttgcggatc agcgcgacgg cacagccgta gaagaacacc agaaggccga aggcgaagat
    13861 gtaggaaccg atcgacgaga tgtagttcca gcccgcgaac gcgtcgggat agtcgacgta
    13921 acggcggggc atgccggcga gcccgaggaa gtgctgcggg aagaacagca cgttcgcgcc
    13981 gatgaaggcg atccagaagt ggagctggcc gatccagtcc ggaatgacat agccggtgat
    14041 cttcgggaac cagtagtacc aggccgcgaa gatcgcgaag accgcgccga gcgacagcac
    14101 gtagtggaaa tgggccacca catagtaggt gtcgtgcaac acgcggtcgg cgccggcgtt
    14161 cgccaggacg acgccggtca cgccgccgac ggtgaacagg aagatgaagc cgatcgccca
    14221 gaccatcggc gcggtgaagc gcagcgaacc accccacatc gtggcgatcc aggagaagat
    14281 cttcacaccg gtcggcacgg cgatcaccat ggtcgcggcg acgaagtact gctgggcacc
    14341 gagcgagatg ccggtggtgt acatgtgatg cgcccagacg atgaagccga cgaacccgat
    14401 cgccaccatc gcgtaggcca tgccgaggta tccgaacaca ggcttgcgcg agaaggtcga
    14461 gacgatctgg ctgatgatgc cgaaggccgg caggatcagg atgtagactt cgggatggcc
    14521 gaagaaccag aacaggtgct ggaacaggat cgggtcaccg ccgccttcgg gagcgaagaa
    14581 cgtcgtgccg aaattgcgat cggtcagcag catcgtgatg ccacccgcga gcaccgggag
    14641 cgacagcacc agcaggaagg ccgtgatcag gatcgaccag acaaacagcg gcatgcggtg
    14701 cagcgtcatg cccggggcgc gcatgttgaa gatcgtcacg ataaagttga tcgcgccaag
    14761 gatcgacgag gcaccggcga tatgcagcga caggatggcg aagtccatcg ccggaccggg
    14821 ctgccccgaa gtcgacagcg gcgggtatat ggtccagccg ccgcccacgc cgttcgcgcc
    14881 cggcgggcct tccacgaaca tcgagatcac aagcagggcc aacgccgacg gcagcagcca
    14941 gaacgagatg ttgttcatgc gcgggaaggc catatccggc gcgccgatca tcagaggcac
    15001 gaaccagttg gcatagccac cgatcatcgc cggcatgacc atgaagaaga tcatgatcag
    15061 gccgtggccg gtgacgaaca cgttgaacgt gtgcgcgctg gcgaaatact gcatccccgg
    15121 ctcctggagc tcaaggcgca tcatgatcga caggaagcca ccaaccagcc ccgccacgat
    15181 ggcgaagatc aggtacatcg tgccgatgtc cttgtggttg gtcgacagca cgtaacggcg
    15241 ccagccggtc gggtggtcgt ggtgatcgtg ggcgtgagcc gcggacgatg ccatgatcta
    15301 aatctcctga aaaatcagct gtagccggtt actgctgcac cgcaggcgtg cccgcgaact
    15361 gcatcgccgg cgacgggtcg gttgcaaatt tcttctgcgc ttctttcagc caggcgtcgt
    15421 atttgtcctg cgagacgacg cggatggcga gcggcatgaa ggcatgatcc ttgccgcaca
    15481 gttcctggca ctgaccgtaa tagatgcctt ccttggtggc cttgaaccag gtctcgttca
    15541 aacgccccgg cacggcgtcg atcttcacgc cgaaggactg gatggcgaag gcgtggatca
    15601 cgtccgcagc cgtgacctga acgcggacca ccttgttgac cggaacgtag gcgatgttgt
    15661 cgacggccag caggcgcggc atgtcctcgg ccttggcccc ggccttgatc gccttggcgc
    15721 ggtcctcgtc gttcagcatg atcgcatcga aggtgatgcc gccgtaatcc ttgggatact
    15781 cgaaggtcca gtaccactgc ttgcccgtca ccttgatggt catgtccgac ttcggaatca
    15841 cgacctgctc gcgcagaagc ttgaacgacg gcaccgcgat gaacaccagg atcagcactg
    15901 gaatgaccgt ccaggccact tccagcatcg tgtgatgcgt ggtcttggag gggaccgggt
    15961 tggccttctc gttgaaccgc acaacgatgt agatcagcag cgcgagcacg aagatcgaga
    16021 tcaccgtgat gatcggcagc agcaggccgt catggaacca ctggatgttc tcggcgatct
    16081 gggtggccgg cggctgcaat cccttctgcc agggcaaggg catgcccttg tcttccgcca
    16141 ggaccgcgct cgcggaagcg aacaccaagg ccgccgcgag accagcgccc gcaaatttcc
    16201 ccgccatctt catgccaaac attggatgta gccgctcctg aagcacaatc gtctcatgaa
    16261 aacggtcgcg cggctttgag aaattcctca gcagtccgcg tgcgaccgcg ggcttcgccg
    16321 tcgcaggcga gtcccctcag tttgactgtc aaacacaagt gccgccatga cgcaacaccg
    16381 ctcagcgccg aacctgccgc cgggggtgcg gcaaaattgc gcgggccgcg cgcgccgttc
    16441 cttgactcgc gcccctgctc tgttacccgc tatgccggat tgaacgggaa gcccatgcct
    16501 ttgccagccc caaacctgtt gcgagccgtg acattgagcg cgtttctggc cgtcgccggc
    16561 ttgaccgcgg cgcctgtcag cgcacagggc gcgctcaagg caacccatgg cgactggcag
    16621 ttgcgctgcg actcgttgcc gggacgctcc ggggagcagt gcatcctcat gcagagcctg
    16681 gttgacgacc agaacgtcgt cgaaggccag aaggtcgaga tcaatctcgt cgtcgtcgcc
    16741 ctcaaggcca gcgacccgaa ggccaataac gcgcgcaagc cgatcctgcg cgtgatcgcg
    16801 ccgctgggcg tgttgttgcc gcgcggcctc ggcctgaaga tcgatacgac cgagatcggc
    16861 gcgaccggat tcgtgcgctg tctcgccaac ggctgtgtcg ccgaagtcga gatggacgac
    16921 gccctcatcg acaagttcaa gcggggcaag gaggcgacct tcgtgatctt cctgaccccg
    16981 gacgaggggc gcgggctgcc ccttagtctt gacgggttcg agaagggttt tgcgcagctt
    17041 cagtgagtgt ttgaccatgc cagtctctct tcgttccgtc ctggttctcg ccctcgggct
    17101 cgctcttgcc ggttgccagt cctccggcgg cgaccaggcg gcggcgagcg gacagccgag
    17161 ttcgtggcgt ctggcattct tcggcaatcc tcaggccgtg cctccggaaa agccgatcga
    17221 atcgtcccag cgggacctga actgtccgag tgtcggcgtg ctggaggggg gagcggctca
    17281 ccgcatcggg cgacagggcg ggagcgaggt ctcgcaccag gcctcgctgg tcgatacggc
    17341 aagggagtgc cgcttcgccg gtaacagcat gacgctcaag gtgggcgttc agggtcgatt
    17401 gctgatcggg gccgcaggca gggctggcac ctacacggtg ccagtgcgcg ttgccgtcaa
    17461 gcgcggcgaa gcggtcgttg cctcccgctt cgcacgcgtt tccgtcacga tccccgccaa
    17521 cgaggccagc gccagtttcg ttcatatcga agacaacatc gcgctgccgg tcagcgataa
    17581 cgatccggcc gaggagtacg acatctacgt cggcttcgac gaaggcggcg gtccggccga
    17641 tccgcgccgc ggcacgcgca gacgctagcg gcgcacgtgc ttttcccgtt gacttggctc
    17701 gccctgccgc attaggtcgc tgcacggcat tcgatcctcg cgggagagac cggctcgtcc
    17761 ggcgccgaag gcgcaaccgc cccggaaacg ctcaggcaaa aggaccgcga gaggaagccg
    17821 ctctggaaag cagccgttca ggctcaccga cggagtaagt gcgcgcccag gcgtgcgcga
    17881 atctctcagg tcacgggaca gagggggcgg tcgtgggttt cgacccatgc ctcgtcatgt
    17941 cgtatccgga gcgcccgatg tccgatcctg ccgcgcctgc aaagcgtacc ccgctttacg
    18001 aatcgcatgt tgccgccggc ggtcgcatgg tgcccttcgc tggctatgaa atgccggtgc
    18061 aatatcccac gggcattttg acggagcaca actggacacg agagaacgcc ggcctgttcg
    18121 acgtgtcgca catgggccag gcgttcctgc tcggccccga tcacgacacg acggcccggg
    18181 cgctggaaac gctttgcccc gccgatttcg tcaatctcgg cctgggaaag cagcgctatt
    18241 cgcagctcct ggcgagcgat ggcggcattc tcgacgacat catggtcacc cgctcagtcg
    18301 atccggcgga ggatggggcc ctgatgctcg tggtcaatgc cgcctgcaag gacgcggact
    18361 tcgccctgat cgagcagcgc ctgccggcga atgtgaagct tctacgcgcc gggcaccgcg
    18421 cccttgtcgc gctgcagggg ccgagagcgg ccgaggtgct ggcgaagcat tgtcctgaca
    18481 gcgccgacat ggcgttcatg tcggcgcgct cgacgcgttt cgacaatttc gactgccacg
    18541 tttcccggtc cggatatacc ggcgaggacg gctatgagat ttcaatcaac gcgaaatacg
    18601 tcgcgacggt ttgggatacg ctctgcgccg atcctctggt caagccgatc ggcctcggcg
    18661 cgcgggattc gctgcgtctc gaagccggcc tgtgcctcta cggccatgac attgatgcga
    18721 cgacctcgcc ggtcgagggc gccctcgcct ggtcgatcca gaagcgacgc cgcgaggaag
    18781 gcggatttcc cggcgccgat cgcatccagc gcgaactgcg ggacgggcct tcgcgcctgc
    18841 gtgtcggcat cctgccggaa gggcgccagc ccgcgcgcga gggggccgag attcatgccg
    18901 atggccgcaa ggtcggcgtg gtcacctccg gcggattcgg tccgacgcgg aacggcccct
    18961 gcgccatggg ctatgtcgag gctgctttcg ccaggcccgg cacggcgctg acactgatcg
    19021 tgcgcgacaa gccgttgccg gccgtcgtcg cgccgatgcc ctttgttccc aaacgttttt
    19081 tccagccgaa ggccggctga taccactccg ggagatccct catgagcacc atccgctaca
    19141 ccaaggacca cgaatacatc cgtgtcgagg gcgatatcgg aacggttggc atttccgact
    19201 acgcgcaggc gcaactcggt gacgttgtgt tcgtcgacct gcccgcgccc ggcaaggagg
    19261 tggcccaggg cgccgagacg gcggtggtcg aaagcgtcaa ggccgccagc gagatctacg
    19321 ccccggtcgc cggcagtgtg gtcgaggtca acgcccggct cgccgacaat ccggcgacgg
    19381 tgaacgaggc gccgcttgga gatggctggt tcctgaagct caggatcgcc gacacggcgc
    19441 agctcgacgc cctgatgacc gaagagcagt accaggacta cctgaagacc ttgggctgac
    19501 attcccgaca gcggagagac accgtgcgtt acctgcccca tacacaggct gaccgggacg
    19561 ccatgctggc gcgcatcggc gtcggctcgg tcgatgagct tttcgccagc atcccggcgg
    19621 agaagcgctt gaaatcgctt ccggatcttc cgcgcgcccg gactgagctg gaggtcgagc
    19681 gcatcctgtc gaagatggcg gggcggaacg tcccggcctc ggccgtgccg ttctttgtcg
    19741 gcgccggcgc ctacaagcat catgtgccgg cgacggtgga tcacctgatc cagcgctcgg
    19801 agttcctgac gagttacacg ccgtatcagc ccgagatcgc ccaaggcacg ctccagtatc
    19861 tctttgaatt tcaaacgcaa gtcgccttgc tgaccggcat ggacgtggcc aatgcctcga
    19921 tgtacgacgg ctccaccggc acggcagaag ccgtgctgat ggcgcatcgc gtcaccaagc
    19981 ggcgcaaggc agtgctttcc ggtggactgc acccgcacta cgtcgatgtg gtgcgaaccc
    20041 tttccgacat ggcaggcgac gacctcgtgg tcctgccgcc ggacgtgacg gctgcggaag
    20101 acctggccgc gacgattgat tccacagtct cctgcgtggt cgtgcaaact ccggatttct
    20161 tcggaaacat cagggatatc agcgctttgt ccgccaagtg caaggccgcc ggcgcgctgc
    20221 tgatcgccgt cttcaccgag gcagtttcgc tcggcgcgct gcaatcgccg ggcgagatgg
    20281 gggccgacat cgtggtcggg gaaggccagt cgatcggcaa cggcctcaat ttcggcggcc
    20341 cctatgtcgg gctgttcgcg acgaagcagc agtatgtgcg ccagatgccg gggcggcttt
    20401 gcggcgagac ggtcgattcg gaaggccggc gcggcttcgt gctgacgctg tcgacgcgcg
    20461 agcagcacat ccgccgcgag aaggcaacgt cgaatatctg cacgaattca gggctttgca
    20521 ccctggcctt caccattcac atgtcgttgc tgggcgaagc gggcctgcgc cggctggcgg
    20581 ccgtgaatca cgccaatgcg gtgacgttgg cggacatgct gtccggcgtg ccgggcgtcg
    20641 aggtgctcac ccggagcttc ttcaacgaat tcacggtgcg gctgaagcag gatgccgccg
    20701 cggtgctcga aaagctcgta gaacgcggca tcctcggggg cgtaccggtc tcgagactgc
    20761 tgccgaaccg ggggctcgat gacctgctcg tcatcgcctc cagcgaggtc aacacggacg
    20821 gcgatcgcgc ggcgctcgcc ggcgccctga aggaggtgct gtgatgctca acaaccaggg
    20881 tcgccccacc gccgccggca gcgccaccac aaccggccat gccaccttca ccggcaatcg
    20941 cgcgctcatg caggaagagg cgctgatctt cgagaccggc cgcctggacg cgaccggcgt
    21001 cgacattgag gaaccggcca aggtggcgaa ccgcctcggc gggctggagc ggaaggcggc
    21061 catcggcctg cccggcctgt ccgagccgga ggcgatgcgc cactacgtca agctgagcca
    21121 gaagaactac ggcatcgaca ccgggttgtt cccgctcggc tcctgcacga tgaagcacaa
    21181 cccgcgcctc aacgagaaga tggcgcggct gccgggcttc aacgacgtgc atccgctcca
    21241 gccgcaatcg acggttcaag gcgcgatcga cgtgatggcg gaactgtcgc gctggctgat
    21301 ggaattgacc ggcatgccgg cggtggcgct cagccccaag gcgggcgccc atggcgaact
    21361 ggccggcatg atggcgatca aggccgcgat cgaagccaag ggcgaaggca agacgcgcaa
    21421 tgtggttctg gtgccggatt cggcccatgg caccaatccg gcgacggcgg cgctgatcgg
    21481 cttttcggtc aagtccgtgc cggccgattc cgatggcacc gtgcgcgtcg ccgaggtgaa
    21541 gaagctcatc ggcccggacg tggcggcgat catgctgacc aatccgaaca cctgcggcct
    21601 gttcgaaccg gaaatcgcgg acatcgccgc agcgatgcat gaggccgggg cgtatttcta
    21661 ttgcgacggc gcgaatttca acgccatcgt cggcaagacg cggccgggcg atctcggcgt
    21721 cgacgccatg cacatcaacc tgcacaagac cttctcgacg ccgcatggcg gcggcggccc
    21781 gggttccggc ccggtcgtgc tgtcggagcg gctcgccccc tatgcgccgg tgccgttcct
    21841 ccacaaggac ggcgaccgga tagtcctggt cgagcatgcg gacgggtcac aggctttcgg
    21901 gcgcgtcacc gccttccacg ggcagatggg catgtatgtg cgtgccctgt cctacatgct
    21961 gtcgcacggg gcggacggca tccggcaggc cagcgaggat gcggtgctca acgccaacta
    22021 catccgcgcc ggcctgtccg acctgatgtc gcagcccttc ggcgacaagc cctgcatgca
    22081 cgaggcgctg ttcgacgatg cctggctgaa ggacacgggc gtgacgacgc tcgacttcgc
    22141 caaggcgatg atcgacgagg gctatcaccc catgaccatg tacttcccgc tggtggtgca
    22201 tggcgcgatg ctgatcgagc cgacggaatc ggaaggccgg gcctcgctcg atctgttcat
    22261 catggcgctg cgcgatctcg ccatggccgc gcggcgcggc gagacggagc ggttcaccgg
    22321 ggcgccgtat cacgcgccga ggcggcgtct cgacgagacg
//
LOCUS       DAOMXN010000018        22325 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_1420_length_22325_cov_13.372205, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000018 DAOMXN010000000
VERSION     DAOMXN010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 22325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22325
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1420_length_22325_cov_13.372205"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1677
                     /locus_tag="PK812_03165"
     CDS             <1..1677
                     /locus_tag="PK812_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRJ68583.1"
                     /translation="RRGRPAPPPPDHSDPYAIYRTPVYNGLRRAVSWSVDHKYVTVGA
                     VLLILAGSIAAFTRVQHQFFPISSRPELFFQMRMPQGTSITASLDIAKRGEAMLKDDK
                     DIATFTTYVGQGSPRFWLGLNPQLPDEAFAEIVIIARDVAARERIKARLEGEVAAGKL
                     SEARVRVDRFNFGPPVGFPVQFRVIGPDPASVRETALKVRDIMRANVAARDPHFDWNE
                     QTPSVRLVVDQDRARALGLNVQDVSNMLQTWLSGVTVTSVRDRTERVDVVARAAPAER
                     ADLSRIGELTIATRGGVPVPLAQVARIEYVHEDPILWRRSRELAITVRADVVDGVQPP
                     TVSHDVGEALKPLIATLPPGMRIEQGGSVEESGKANSSIFMLFPLMILGMLTILMLQL
                     QSFSRLFLVFAPAPLGIVGASIGLNLFNAPFGFVALLGLIALAGMDMRNTVILVQQID
                     EDLAAGADMREAVIEATVRRARPVVLTALSAILAMIPLSTSLFWGPMAITIAGGLATA
                     TFMTLLFLPALYSIWFKVQPARNRAPVVAPPSPEQVQDEALTMITRMAAE"
     gene            1758..2453
                     /gene="pyrF"
                     /locus_tag="PK812_03170"
     CDS             1758..2453
                     /gene="pyrF"
                     /locus_tag="PK812_03170"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HRJ68584.1"
                     /translation="MTDARDRMIVALDLPSVAEARAMVTRLGHSASFYKIGKELVYAG
                     GLDIAQELVAAGKKVFLDLKLHDIPNTVERAAAAVSGLGATYLTVHAYPQTLAAAVRG
                     AGKGGLKVLGVSVLTSMNDADLALAGYGLGVADLVRRRARQTMESGAHGLICSPLDIA
                     GVRETVGRALEIVTPGVRPAGSAAGDQKRVMTPRDAIRAGADRLVVGRPVTEAADPKA
                     AAEAIVAEIAAAG"
     gene            2513..2821
                     /locus_tag="PK812_03175"
     CDS             2513..2821
                     /locus_tag="PK812_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HRJ68585.1"
                     /translation="MAKGYWIARVDVYDMDSYKQYAALNQIPFKKYGARYLVRGGAFE
                     LKEGGSRARNVVIEFPTYQAALDCYHSPEYKEACKHRGGWISSGDLIIIEGYEGAQPA
                     "
     gene            2841..3653
                     /gene="dapB"
                     /locus_tag="PK812_03180"
     CDS             2841..3653
                     /gene="dapB"
                     /locus_tag="PK812_03180"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HRJ68586.1"
                     /translation="MGDLRLAVVGAGGRMGRTLVRAITEAGGVRLSGALERPGAPALG
                     QDAGLLAGLPALGVVVTDDINTAFADADGIIDFTAPEATRAFAACAAEKGLIHIIGTT
                     GLAEADFAAISDAGRTAVVVQSGNMSLGVNLLAALVKRAAAALGPEFDIEIVEMHHRH
                     KVDAPSGTALLLGQAAAQGRGIDLGAHSARGRNGHTGARRTGDIGFASLRGGSVIGDH
                     TVTFAGSGERIELTHRAEDRGLFAVGAVKAALWARGRPPGVYSMADVLGIAD"
     gene            3660..4187
                     /locus_tag="PK812_03185"
     CDS             3660..4187
                     /locus_tag="PK812_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012482305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRJ68587.1"
                     /translation="MAHIRRLSAPEAESHLDDLADVLADCVSGGAGVGFVLPFSKDDA
                     KAWWRSIVPGVASGERLLFAAFEGDDLVGTVQLCPAGMPNQTHRADIAKMLVHSRARR
                     KGIGAALMKAAEAHARSMGRFVLVLDTVTTSDARRLYERLGWQVAGPVPRFARSPQGG
                     WDDTLFMWKALDDGA"
     gene            4174..4830
                     /locus_tag="PK812_03190"
     CDS             4174..4830
                     /locus_tag="PK812_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HRJ68588.1"
                     /translation="MTAPEGAACNSAGVALQPGLILHRAWFGRSAQEQLVETVRRVVR
                     DAPLFTPRMPRTGKPFSVRMTNCGPLGWVSDAEGYRYQAAHPDSGVCWPPMPEGLLAL
                     WSAVAGYPHPPEACLVNLYDRAARMGLHQDRDEADFAAPVVSVSLGASALFRFGGTAR
                     TAPTRSFRLESGDVLVIGGESRLCFHGVDRIYPGTSTLVPEDQRINLTLRRVTRPQRS
                     "
     gene            4958..6055
                     /locus_tag="PK812_03195"
     CDS             4958..6055
                     /locus_tag="PK812_03195"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HRJ68589.1"
                     /translation="MATSTVLGIGGLVVGLVFGAIVQRTNFCTLGAVSDLILMEDGRR
                     MRAWVLAAAVALFGTQAMHLAGLINIDSSFYLTSNLGWAGAILGGLMFGFGMTKTGGC
                     AGRTLVRLGAGNLKSLVVLMVMGMVAYMTLRGIIAPLRAAMETSLNYDLKGIGLKTQG
                     VGEMFSRFGGLSPVAGRVVASVVVGGALLWYALKDADFRRSGRDVFAGVGIGLTILGG
                     WTVTGILAKDEFAPVPLTSLSFASPIAEFLQYLMVFTGTTINFGAALVAGVVLGSFLM
                     AAGTGRFRIESFADKDDLVRHLGGATLMGIGAILSLGCTIGQGMTGTATLAFGGMIAW
                     ASIIAGAYYGVKYLEEGTYIGALKASFGASS"
     gene            complement(6060..6422)
                     /locus_tag="PK812_03200"
     CDS             complement(6060..6422)
                     /locus_tag="PK812_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HRJ68590.1"
                     /translation="MIPKAAPTPSEIRLKDKGRLLVVSFPDGASYELPAELLRVESPS
                     AEVQGHSPSEKRIVPGKKDVAILRIEPVGRYAVRLTFDDGHDSGIYAWDTFVSMGTNR
                     EGLFAAYLEALKARGLSR"
     gene            complement(6427..7548)
                     /locus_tag="PK812_03205"
     CDS             complement(6427..7548)
                     /locus_tag="PK812_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HRJ68591.1"
                     /translation="MITKDSVLAALASVPGPDGRTPLGQSGGVTNISVQDSRVFIAIT
                     VDPAFPRTQPVEMDMLRKRVEAAAKTAPGVLSAIVTFTAERPTTNAPAGRPKARGQGD
                     PQGDPVPGVKHIVAVASGKGGVGKSTTAANLAVSLAALGLRVGVLDADIYGPSMPRLF
                     GLTAKPDVNDEGQLIPLENHGCKVMSIGFLVDENTPMVWRGPMVMSALTQLLRDVAWG
                     DLDVMVVDMPPGTGDAQLTMAQTVPLSGAVIVSTPQDLALLDARRGIAMFERVNVPIL
                     GLVENMSYFVCPHCGERSDIFAHGGAKREAETMNVPFLGEVPLEMAIRETSDGGQPIA
                     STAPNGPFAAIYANVARELSDQLALTEPAARPQLKIVVE"
     gene            complement(7578..9002)
                     /gene="cysG"
                     /locus_tag="PK812_03210"
     CDS             complement(7578..9002)
                     /gene="cysG"
                     /locus_tag="PK812_03210"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF007922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HRJ68592.1"
                     /translation="MNVLPIYFDVRNKPVAVVGGGTVASRKAEMALRAGATVTAYAAE
                     LGEDFRGVKDHPNFRHQARAIEASDVDPCALVYCAVEDQETAERVHALAKAARVPVNV
                     ADNPPLCDFSMPTILDRSPVVVAIGTEGASPILGRMLKARLEAAVPASYGRLAAFVRT
                     FRDALKDKVTDGAGRRRFWERIMEGPVAEMVLAGKEKEAEAALNAAIEAEGTRDTPAM
                     GEVYLVGAGPGDPDLLTFRALRLMQKCDVVLYDRLVSDGILNLVRRDSERIYVGKQRA
                     DHALPQEDISSLLVTLARQGKRVLRLKGGDPFIFGRGGEEIEMLAAHHIPFQVVPGVT
                     AAAGCAAYSGIPLTHRDHAHSCLFVTGHGKDGDISADWTTLIQPRQTVAIYMGLAKLE
                     RLTADFIAHGADPQTPAAIIENGTRENQRVVVGTIRSLVEDARREGLKGPTIIIIGSV
                     VTLRDKLNWFASADRAQFRQAATA"
     gene            complement(8999..9223)
                     /locus_tag="PK812_03215"
     CDS             complement(8999..9223)
                     /locus_tag="PK812_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68593.1"
                     /translation="MSVENRTPIVEIAAPYRRAIRLEEVDFESGMRMLRVVLREGHRI
                     TQIDLDAETAARWGTMMADWAARQPGGDTA"
     gene            complement(9220..9429)
                     /locus_tag="PK812_03220"
     CDS             complement(9220..9429)
                     /locus_tag="PK812_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur relay protein DsrC"
                     /protein_id="HRJ68594.1"
                     /translation="MANLSDLIIQHPEITSFYELERLVVESARSGMILLYFDLKPDYA
                     DTPRDWQNRLEMAFYRVETRPGKKR"
     gene            complement(9431..10420)
                     /locus_tag="PK812_03225"
     CDS             complement(9431..10420)
                     /locus_tag="PK812_03225"
                     /inference="COORDINATES: protein motif:HMM:NF014698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HRJ68595.1"
                     /translation="MSDVPRMIPSTSMPTARITAAGAGSGPFSLADETAYRAWRAARL
                     DAAPKQADDLIIPVADLAQPTESELAALRAMVRRANMAVYRSPALAGHAESARRALAR
                     FAQIAGLTEFEEHRSSGPDGIVPIEVVEEGLRAGFIPFSNRAINWHTDGYYNYRGPDR
                     CIRSMALHCVRSAAAGGESGLFDPEIAYIRLRDRNPDYIRALMHPEAMTIPAHEEATV
                     SAHGAVAGPVFVAHPTTGALIMRFTIRKRNVTWRDDPVLAAALEALADILANDPLVYR
                     LRLEPGMGVICNNVLHDRRAFDNAAADTADGGRLLFRIRSYNAVFETGRPARH"
     gene            10848..12179
                     /gene="dsrA"
                     /locus_tag="PK812_03230"
     CDS             10848..12179
                     /gene="dsrA"
                     /locus_tag="PK812_03230"
                     /EC_number="1.8.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018551 - hydrogensulfite reductase
                     activity [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dissimilatory-type sulfite reductase subunit
                     alpha"
                     /protein_id="HRJ68596.1"
                     /translation="MADDKMANESKGGDCGSGGCGCKTAARPTPNLDQLESGPWPSFV
                     TGLKRLRDSGGKKYATMMNDLLGQLEHSYTNRLGYWKGGTVSVFGYGGGVIPRFSEVA
                     DKFPESKEFHTLRVQPPAGFHYNTDVLRQMCDIWEEHGSGLIAFHGQSGDIMFQGTST
                     ENTQKAFDALNAIGFDLGGAGPALRTAMSCVGHARCEMSCYDEVKAHRSVINSFLDEM
                     HRPALPYKFKFKFSGCPNDCVNAIHRADFAVIGTWRDDMKVDQEEVKAFVAKKGRKEM
                     IDTVIAMCPTRALSLNDDDTLDVDNKSCVRCMHCLNVMTKALSPGDDRGATILIGGKR
                     SLKIGDLMGIVVVPFMPLNTDEDHENLVEFARKVLDFFAENALEHERIGETIDRIGLP
                     FFLEAIGVEPDPNMVRHPRTSSYVRTDNFTEEAEKYFERKAAKNAAKTAAE"
     gene            12266..13360
                     /gene="dsrB"
                     /locus_tag="PK812_03235"
     CDS             12266..13360
                     /gene="dsrB"
                     /locus_tag="PK812_03235"
                     /EC_number="1.8.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018551 - hydrogensulfite reductase
                     activity [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dissimilatory-type sulfite reductase subunit
                     beta"
                     /protein_id="HRJ68597.1"
                     /translation="MSQVQANAPAKPRLPDEHGVPDPFQYMHPVLKKNYGQWAYHDRP
                     RPGVLHHVSKTGDEVWTVRAGTQRQMDVYTIRKLCDIADQVGEGHVRFTTRSNIEYMV
                     SRFDKVPALIKALNEAGFPVGGTGNSVSMISHTQGWLHCDIPGTDASGVVKSLMDMMY
                     EEFIKEEMPNRVRITTSCCQINCGGQGDIAINVQYTKPPKINHDLVANVCERPAVVAR
                     CPVAAIRPAMVNGKPSLEVDERKCVCCGACYPPCPPMQINGADSTKIAIWVGGKHSNA
                     RSKPTFHKLVAANLPNNAPRWPEVAEVVRKILYAYKEDGRYWERMGEWIERIGWPRFF
                     EMTGLAFTPHHIDTWVGARHNMNMSTHIHF"
     gene            13386..13778
                     /gene="tusD"
                     /locus_tag="PK812_03240"
     CDS             13386..13778
                     /gene="tusD"
                     /locus_tag="PK812_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HRJ68598.1"
                     /translation="MKFGIVVNEGPYTHQASDTAYQFAKAALDKGHEVVRVFFYHDGV
                     NNATRLTVPPQDERNVQRNWSELGAKHNLDMVVCIAAAQRRGILDENEAKRQGKDANN
                     IAPGFRISGLGQLIEAGILADRLVVFGD"
     gene            13789..14193
                     /gene="tusC"
                     /locus_tag="PK812_03245"
     CDS             13789..14193
                     /gene="tusC"
                     /locus_tag="PK812_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HRJ68599.1"
                     /translation="MAQAQKKFLYVNRKAPHGSIYALESLEVVLIGAAFEQAVSLAFL
                     DDGVFQLARGQNTKGIGAKNFSGTYRALGDYDVRDLYVERESLEERGLSTDDLLEITH
                     DTDDEEDIPSIKIVSRAEMADIMASQDVILSF"
     gene            14203..14511
                     /gene="tusB"
                     /locus_tag="PK812_03250"
     CDS             14203..14511
                     /gene="tusB"
                     /locus_tag="PK812_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HRJ68600.1"
                     /translation="MSTLHTVNKSPFERNSLSSCLGHCKPGDAVLLIEDAVVGARKGG
                     SFAGRLAEAGKQCSMYALGPDLAARGLKPEDMDACVQVVDYGGFVDLAAEHARTQAWL
                     "
     gene            14569..14901
                     /locus_tag="PK812_03255"
     CDS             14569..14901
                     /locus_tag="PK812_03255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HRJ68601.1"
                     /translation="MGYHFNGQDFAADEEGYLTDLSVWSPELAGLIAKDENIDMTPEH
                     WEVVNFLREYYDEYQIAPAIRVLTKALAKRMGPDKSSNKYLYELFPYGPAKQACKIAG
                     LPKPTGCV"
     gene            14973..15722
                     /locus_tag="PK812_03260"
     CDS             14973..15722
                     /locus_tag="PK812_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HRJ68602.1"
                     /translation="MTALTIAYVMLFYAATLVLVGGVGYRIWQFATTPAPLKIPTTPA
                     PVTTSGVAFRLAREVVLFESLFKANKWIWVFGWVFHAALLLVLLRHVRYFQHPVWDVI
                     ALVQPVGVYAGFAMVVGLLGLWARRFLVERVRYISSPSDHLMLALLVLIGASGLMMKF
                     VAHTDIVAVKAFFLGLMRFEFQPLPADPPLLVHLTLVAALMIVFPFSKLLHAPGVFFS
                     PTRNQADNPRERRHLARWAAALGDGPTRRQP"
     gene            15719..17245
                     /locus_tag="PK812_03265"
     CDS             15719..17245
                     /locus_tag="PK812_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRJ68603.1"
                     /translation="MTDARDAKVPDSPPDLPDPLEMPAIACGAMAHSRPYPATPAMQQ
                     ALGFAGELVPDWKNKAIAKMGDLVGKYRSLKVYLDSCVKCGACTDKCHYFLGTGDPKN
                     MPVARQDLFRKVYRRHFTLAGKYAPWLVGAEDLTEEVLQDWNTYFHQCSQCRRCSVYC
                     PYGIDTAEISMAAREIMDHIGLGQKYSNEIIGKVFKVGNNLGLPPKALRATLEGLEEE
                     IEEDTGAKVRLPLDEAGADILLVTPSADFFASPHMEGLIGYAKVFHAAGAKWTISSKA
                     SEAANFGMFVGSYELMRDISLRIREAALELGVKRIVIGECGHAWRVAYSFLNTLAGPF
                     DFLDPNYPKPQHICEYTWDLIQDGKLTFDKSKNDHMKLTFHDSCNVARASRMGPTPGG
                     QFEIPRNIIKAVCNHFYDMAPETTREATFCCGGGGGLLSDELMDLRIKGAQPRMRALE
                     EVVKTNGVTHMAAICAICKAQFSKVLPEYNFDREMIVSVHQLVSNAIVLERAPDPDGE
                     "
     gene            17281..19236
                     /locus_tag="PK812_03270"
     CDS             17281..19236
                     /locus_tag="PK812_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HRJ68604.1"
                     /translation="MNETVAPKPHTFRKFKDGGNMWTDWSDLQQKIFNADKSHKCPTY
                     VHRTPPCQGSCPSGHDIRGWLAIARGQDKPPVAGMPWQHYAFERMLDANPFPAAMGRV
                     CPAPCEDGCNRNEVDDFVGINGVEQYVGDWAIQNGLKAPAAAADTGRKIAVIGGGPAG
                     LAAAFFLRRSGHAVTIFEAHDKLGGMMRFGIPGYRTPRTMLDAEIGRILDMGVQVRLN
                     TRVGWDVPMAELESGFDAIFWAVGAQKGRPLPVPGADAPNCMTGVDFLDAFNQGWVFS
                     TARNIVVVGGGDTSIDVASVARRLGHISQENAKDMSTHELLGYTAHDVAGSLRREGVK
                     ATLTSLFPIEKMTAAEREREDALREGVDIKGGVMPLEVIKNAEGHAIALKMCQCDMKG
                     MTPVPREGTEFEIPCDMIISAIGQMGDLGGELAALDNGRGFIAIDAVYKVKNKDKHFA
                     GGDVIRPHLLTTAIGHGRIAAESINAFLKGEATDKRPKVDVHHFNLLEELQQRNLAPE
                     AYDHQQKRGTSEARYAIHNYEDRGAASIIPHKDLFKGHFAFMERAKRDEKHVEAENVI
                     GNFEERIIGFTEAQAKREGERCMSCGMCFECDNCVIYCPQTAVGRVPKKERALGRYVY
                     TDYTKCIGCHICADVCPTGYIQMGLGD"
     gene            19239..19718
                     /locus_tag="PK812_03275"
     CDS             19239..19718
                     /locus_tag="PK812_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68605.1"
                     /translation="MRGLCIPLRWAHRLALLAVLFFAPALAGAQQAGRTPHPEVPKAS
                     GGQCVAEKDFMRKNHMKLLNHQRDETVHDGLRPADRSLKACIACHAVPGADGRPVTVA
                     DPKHFCRACHDYVAVKVDCFECHASRPDAADRKAALPLPMGHGDVARIGDYLKEIAR"
     gene            19715..20515
                     /locus_tag="PK812_03280"
     CDS             19715..20515
                     /locus_tag="PK812_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRJ68606.1"
                     /translation="MTAPSQASCQCGKPSGGCASARPQGTRRDFLKDAAGFASATLAP
                     GVTLMAFGDTAQARAPNEKVSAKTRWGMLIDTTKCADGCTACVTACSTENGWKDTGHP
                     ETDAQWIRKVTLRNPRNGATRALPVMCQHCASPPCVDVCPTGASFKRADGIVLVDKHL
                     CIGCRYCMMACPYNARSFIHEALEDQKPHAPRGKGTVESCTLCVHRVDAGRAPACVEA
                     CGSAGHKAMLFGDLNDPKSEIARAVAEAPVSQIRADLGVDPGVMYRGI"
     gene            20531..21721
                     /gene="nrfD"
                     /locus_tag="PK812_03285"
     CDS             20531..21721
                     /gene="nrfD"
                     /locus_tag="PK812_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HRJ68607.1"
                     /translation="MAHVGYREASAGPGFWAAVFGLGVLVLAGLGSAWYMEHHGHIVT
                     GMTNRIVWGMPHVFAIFLIVGASGVLNVASLASVFGKMPYKPKARLSGLLAIAMLMSG
                     LIVLVLDLGRPDRLTVAMTSYNFRSIFAWNIFLYTGFLVIVGAYLVVQMTRRWQGWTG
                     AVGVTAFLWRLILTTGTGSIFGWLVAREAYNAAVMAPLFIAMSLNIGLAVFILVRMSV
                     AAGETHDLTDALLERMARLLGIFIAAVLYFTAVQHLTNLYSASRIGVERFLLVDGGIY
                     PMLFWGVQIVLGSLVPLAIVFSPRTARSHPMIALAAVLVMIGGLAQLYVIIIGGQAYP
                     LQLFPGMEVSSSYHDGVVARYVPSLPEFGLGLGGLALSFLITFIGVRVLKILPVNVGA
                     SAGA"
     gene            21718..>22325
                     /locus_tag="PK812_03290"
     CDS             21718..>22325
                     /locus_tag="PK812_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRJ68608.1"
                     /translation="MNRLLVSAAHRSSGKTTVSIGLAAAFSARGERLRPFKKGPDFID
                     PLWLSRAAAVPCYNLDFNTQSADEIRALFAWASSGCDLALIEGNKGLHDGMDVEGADS
                     SAALAKLLDAPVLLVLDVTGITRGVAPLLMGQAAFDPDVNVCGVVLNRVVSARQEAKL
                     RQAIERYSAIPVLGALPRDGDLLVKERPLGLTTPGDAGGVEAR"
BASE COUNT         3730 a         7235 c         7369 g         3991 t
ORIGIN      
        1 agacgcggcc ggcctgcgcc gccgccgccc gaccattccg atccctacgc catctatcgc
       61 acgccggtct acaacgggct gcgccgggcc gtgtcctggt cggttgacca caagtatgtg
      121 acggtcggcg ctgtgctgct catccttgcc gggtcgatcg ccgccttcac ccgcgttcaa
      181 catcagtttt tcccgatttc ctcgcgtccc gaactgtttt tccagatgcg catgccgcag
      241 ggcacgtcga tcacggcgtc gctggacatc gccaagcgcg gcgaggcgat gctgaaggat
      301 gacaaggaca tcgccacctt caccacctac gttgggcagg ggtcgccgcg cttctggctc
      361 ggactgaacc cgcagttgcc tgacgaggcc ttcgccgaga tcgtgatcat cgccagggac
      421 gtggcggccc gcgagcgcat caaggcacgc cttgaaggcg aggtcgcggc gggcaagctg
      481 tccgaagcgc gtgttcgcgt cgatcggttc aatttcggcc caccggtcgg ctttccggtc
      541 cagttccggg tgatcggccc cgatcccgcc tcggtccgcg aaaccgcgct gaaggtccgg
      601 gacatcatgc gcgccaacgt ggcggcgcgc gacccgcatt ttgactggaa cgagcagacg
      661 ccgtccgtcc gcctcgttgt cgaccaggat cgggcgcgtg ccctcggtct gaacgtccag
      721 gatgtctcga acatgctgca gacctggttg tcgggcgtta cggtcacctc ggtccgcgac
      781 cgcaccgagc gggtagacgt cgtggcccgc gcggcgccgg cggaacgcgc cgacctgtca
      841 cggatcggcg aactcacgat cgccacccgg ggcggtgtcc cggtgccgct cgcccaggtc
      901 gccaggatcg aatacgtcca tgaggacccg atcctgtggc ggcgcagccg cgaactggcg
      961 atcacggtgc gcgccgatgt ggtcgatggc gtgcagccgc cgacggtgag ccacgatgtg
     1021 ggcgaggcac tgaagccgct catcgcgacg cttcccccgg gcatgcgcat cgaacagggc
     1081 ggctcggtgg aggaatcggg aaaagccaac agttcgatct tcatgctgtt tccgctgatg
     1141 atccttggca tgttgacgat cctcatgttg cagttgcagt ccttctcgcg gctgttcctg
     1201 gtctttgcac cggctccgct cggcattgtc ggcgcctcga tcgggctgaa cctgttcaat
     1261 gcgcctttcg gcttcgtggc gctcctcggc ctgatcgcgc ttgccggaat ggacatgcgc
     1321 aacaccgtca tcctcgtgca gcagatcgat gaggatctcg ccgccggagc ggatatgcgc
     1381 gaggcggtga tcgaagcgac ggtccgtcgc gcgcggccgg tcgtccttac cgcgctgtcg
     1441 gcgatcctgg cgatgattcc actgtcgacc agtctgttct gggggccgat ggctataacg
     1501 atcgctggcg gcctggccac cgcgaccttc atgaccctgc tgttcctgcc cgcgctgtat
     1561 tcgatttggt tcaaggttca gccggccagg aaccgcgcgc ctgtggtcgc gccgccttcg
     1621 cccgagcagg tgcaggacga agcgctgacc atgatcacac gcatggcggc ggagtaggca
     1681 ggagcctatt gcgtcgcgcc ggggcggcga atcgtgctta agcggtgccg cccccttcga
     1741 ccgctggagt tttgctgatg actgatgcgc gcgaccgcat gattgtggct ctggacctgc
     1801 cgtccgttgc ggaagcgcgc gccatggtga cccgcctcgg gcattccgcg agcttctaca
     1861 agatcggcaa ggagctggtt tatgccggag gcctcgacat cgcgcaggaa ctcgtcgccg
     1921 ccggcaagaa ggtatttctt gatctgaaac tgcacgatat ccccaacacg gtcgaacgcg
     1981 ctgctgcggc ggtctccggg ctgggcgcca catatctgac ggttcatgcc tatcctcaga
     2041 cgctggctgc cgccgtgcgg ggtgcgggga agggcggtct caaggttctt ggcgtcagcg
     2101 tgctgacatc gatgaacgat gctgatctgg cgttggccgg atacggtctc ggcgttgccg
     2161 atctcgtgcg ccggcgcgca cgtcagacca tggagtccgg cgcgcacggc cttatctgct
     2221 cgcccctcga cattgcgggc gtgcgcgaga ccgtgggtcg cgcgctggag atcgtcacac
     2281 cgggcgtgcg ccccgcaggc agcgccgccg gagaccagaa gcgggtcatg actccgcgtg
     2341 atgccatccg tgccggtgcc gaccggctcg tcgtcggtcg cccggtgacc gaggcggcgg
     2401 atcccaaggc cgcggccgag gcgattgttg cggaaatcgc cgccgccggt tgaccgccgg
     2461 ggcgctggca tgccctaaag gtcgtccatg ggtttcatac ggagttcgca gcatggccaa
     2521 gggctattgg atcgcgcggg tcgatgtcta tgacatggac agctacaagc agtatgcggc
     2581 gctcaaccag attcctttca agaagtatgg cgcgcgctat ctcgttcgcg gcggcgcctt
     2641 cgaactgaag gagggcgggt cgcgtgcgcg caatgttgtg atcgagtttc cgacctatca
     2701 ggccgcgctc gattgctatc actcgccgga atacaaggaa gcctgcaagc acaggggcgg
     2761 ctggatctcc tccggcgacc tgatcatcat cgaaggctat gaaggcgctc agccggcctg
     2821 accacgacgg aaccacggga atgggtgatc tgagacttgc cgtcgtcggg gcaggcggcc
     2881 gtatggggcg cacgctggtc agggcgatta ccgaagccgg cggcgtacgc ctgagcggcg
     2941 cactggagcg tcccggcgcg cccgccctcg ggcaggatgc tgggctgctt gcagggttgc
     3001 cggcccttgg cgtcgttgtg acggacgaca taaacacggc cttcgccgac gcggacggca
     3061 tcatcgattt cacggcgccc gaagcaaccc gcgctttcgc cgcctgtgcc gctgagaagg
     3121 ggctgatcca catcatcggc accactgggc tggcggaggc cgatttcgcc gccatctcgg
     3181 atgccggtcg cacagcggtc gtggtgcagt ccggcaacat gagcctggga gtcaatctcc
     3241 tggccgcgct ggtgaaacgg gcggcggcgg cgctcgggcc ggagtttgac atcgagattg
     3301 tcgagatgca ccaccgtcac aaggtggatg cgccatcggg cacggcgctg ctgttggggc
     3361 aggcggcggc tcaggggcgc ggcatcgatc tcggcgcgca ttcggcacgc ggccgcaatg
     3421 gccacaccgg cgcgcgccgg accggagaca tcggattcgc ctcgctgcgt ggaggatcgg
     3481 tgattggcga ccataccgtg accttcgccg gctccggcga gcgcattgag ctgacccacc
     3541 gcgccgagga tcgtgggctc ttcgccgtcg gcgccgtcaa ggcggcgctc tgggcgcgcg
     3601 gcaggccacc cggtgtctat tcgatggcgg acgttcttgg catagcggat tgagccatga
     3661 tcgcgcatat ccgccgtctg tccgctccgg aggccgaatc ccatctcgac gacctcgcgg
     3721 acgtgcttgc cgattgcgtg tcgggtgggg ccggcgtcgg cttcgtcctg ccgttttcga
     3781 aggatgatgc gaaggcatgg tggcgctcca tcgtgccggg tgtcgcctcc ggtgagcgcc
     3841 tgctgttcgc ggcctttgag ggggatgacc tcgtcggcac ggtgcaactc tgcccggccg
     3901 gcatgccgaa ccagacccat cgcgccgata ttgccaagat gctcgtccat tcccgcgccc
     3961 gccggaaggg cattggcgcg gcgctgatga aggccgcgga agctcatgcc aggtccatgg
     4021 gccgcttcgt gctcgtcctg gacacggtga ccacgagcga tgcgcgccgg ctttacgaac
     4081 gtctcggctg gcaggttgcc ggcccggttc cgcgtttcgc acgctcgccg cagggcggat
     4141 gggatgacac cctgttcatg tggaaggcgc tggatgacgg cgcctgaggg ggcggcttgc
     4201 aacagcgccg gggtcgcgct gcagccgggc ctcatcctgc atcgtgcttg gttcggtcgg
     4261 tcggcacagg agcagttggt cgaaacggtg cggcgcgttg tccgggacgc cccgctgttt
     4321 acgcccagga tgccgcgcac cggcaagcca ttctcggtaa ggatgacgaa ctgcgggccg
     4381 ctcggctggg tatccgacgc ggaaggctac cgctatcagg ccgcacaccc ggattcgggc
     4441 gtctgctggc ctcccatgcc ggaggggctg ctcgcgctct ggagcgccgt ggccggctat
     4501 ccccatccgc cggaagcctg cctggtcaac ctttatgatc gcgccgcgcg catgggtctg
     4561 catcaggatc gcgacgaggc cgacttcgcg gcgcccgtcg tgtcggtgtc gctcggcgcc
     4621 agcgcgctgt tccgcttcgg agggacggca cgcaccgcgc cgacgcgctc gttccgtctg
     4681 gaaagcggcg atgttctggt catcgggggc gagtcgcgcc tgtgtttcca cggcgtcgac
     4741 cggatctatc ccggaacctc gacgctggtg cccgaggacc agcggatcaa tctgaccctg
     4801 cgccgtgtca cgcggcctca acgctcctga tcggagcacg agctgttcaa ttcgtcgcgg
     4861 cctgctacct tgcgtgacat ataaactttc gcatatgatt atgtaacgaa gcgggcatgc
     4921 gcagatcccg caacagcggg tggaagcgat ggacggcttg gcgacttcga cggttctcgg
     4981 catcggcggt ctggttgtcg gccttgtgtt tggcgccatc gtgcagcgca cgaatttctg
     5041 cacgctcggc gccgtgtccg atctgatcct gatggaagac ggccgccgta tgcgggcctg
     5101 ggttctggcg gcggcggttg cgctgttcgg tacgcaggcg atgcatcttg ccggcctgat
     5161 caacatcgat tcgagtttct acctcaccag caatctgggc tgggccggtg cgatccttgg
     5221 cggcctgatg ttcggcttcg gcatgaccaa gaccgggggc tgcgccgggc gcacgctggt
     5281 tcgccttggc gctggaaatc tgaagtcgct cgttgtgctg atggtcatgg gcatggtggc
     5341 ctacatgacc ctgcgcggca tcatcgcgcc gctgcgcgcc gcgatggaga caagcctgaa
     5401 ctatgacctg aaaggcatcg gcctgaagac gcagggtgtc ggcgagatgt tttcccgctt
     5461 cggtggtctt tcgccggttg ccgggcgggt tgtcgcaagt gtcgtcgtgg ggggcgcgct
     5521 gctctggtat gcgctgaagg acgccgattt ccgccggtcc ggccgtgatg ttttcgccgg
     5581 cgtcggcatc gggctgacca ttcttggcgg ctggacggtg acaggaatcc tggcgaagga
     5641 tgaattcgct ccggtgccgc tgacctcgct cagttttgct tcgcccattg ccgagttcct
     5701 gcaatacctg atggtgttca ccggtacgac gatcaatttc ggcgccgccc tggtcgccgg
     5761 ggttgtgctc ggcagcttcc tgatggcagc ggggaccgga cggttccgga tcgagagctt
     5821 tgccgacaag gacgacctgg tgcgccatct cggcggcgcg acgttgatgg gcatcggcgc
     5881 catcctgtcg ctgggctgca cgatcggtca gggcatgacc ggcacggcga cgctggcgtt
     5941 cggcggcatg atcgcctggg cctcgatcat cgccggcgcc tactatgggg tgaagtacct
     6001 ggaggaaggc acctatatcg gcgcgttgaa ggcctcgttt ggagcatcgt cctgatgcgt
     6061 caccggctga gcccgcgtgc tttcagcgct tcgagatagg cggcgaacaa gccttccctg
     6121 ttggtgccca tgctgacgaa cgtgtcccac gcgtagatgc cggaatcgtg cccgtcgtcg
     6181 aaggtcaggc gcacggcata gcgtcccacg ggttcgatcc ggagaatggc gacgtccttc
     6241 ttgcccggaa cgatgcgctt ttcggacggc gaatgcccct gcacctcggc cgaagggctc
     6301 tcgacgcgca ggagttcggc cggaagctcg tagcttgccc cgtccgggaa ggagacgacg
     6361 agcagccggc ccttatcctt gagccggatc tcgctgggag tcggcgcagc cttgggaatc
     6421 atcgcctcac tccaccacga tcttgagctg cggccgcgcg gccggttcgg tcagcgccag
     6481 ctggtccgac agttcgcgcg ccacgttggc gtagatcgcc gcgaacgggc cgttcggtgc
     6541 ggtcgacgcg atcggctggc caccgtccga tgtctcgcgg atcgccatct ccagcggcac
     6601 ctcgccgagg aacggcacgt tcatcgtctc ggcttcccgc ttcgcgccgc catgggcgaa
     6661 gatgtccgag cgttcgccgc aatgcgggca gacgaaatag ctcatgttct ccacgagacc
     6721 gaggatcggc acattgacgc gttcgaacat ggcgatgccg cgccgggcgt cgagcagggc
     6781 gagatcctgc ggcgtcgaca cgatcaccgc gccggagagt ggcacggtct gggccatggt
     6841 aagctgcgcg tcaccggtac ctggcggcat gtcgaccacc attacgtcaa ggtcgcccca
     6901 ggcgacatcg cgaaggagct gcgtgagcgc cgacatgacc atcggtccgc gccagaccat
     6961 tggtgtgttc tcgtcgacga ggaagccgat cgacatgacc ttgcagccat gattctccag
     7021 cggaatgagc tgtccctcgt cattgacgtc cggcttggcg gtcaggccga acagccgcgg
     7081 catcgacgga ccgtagatgt ccgcgtcgag cacgccgact cttagaccaa gggccgcgag
     7141 actgacggcg agattggccg ccgtggtcga cttgccgacg ccgcccttgc cggaggcaac
     7201 ggcgacgatg tgcttcactc cgggcaccgg gtcgccctgt ggatcaccct ggccgcgcgc
     7261 cttcggcctg ccggccgggg cgttggtggt cggccgttcg gcggtgaagg tgacgatggc
     7321 gctgagcacg cctggcgcgg tcttcgcagc cgcctcgacg cgcttgcgca acatgtccat
     7381 ctcgaccggc tgggtccgcg gaaaggcggg gtcgacggtg atggcgatga atacgcggct
     7441 gtcctgaacc gatatgttgg tgacgccgcc gctttggcca agcggcgtcc tgccatcggg
     7501 gccgggcacg ctggcgagcg ccgccagaac ggaatccttg gtaatcatgt ggtctttccc
     7561 ctcgaacttt cagatggtca ggcggtcgcc gcctgccgga actgcgcccg gtcagcgctg
     7621 gcgaaccagt tgagcttgtc gcgcaacgtc acgacgctgc cgatgatgat gatggtcggt
     7681 cctttcaggc cctcgcgccg ggcatcctcg accagagacc ggatggtgcc gacgacaacg
     7741 cgctggttct cacgcgtgcc gttctcgatg atcgccgccg gcgtttgcgg atcggcacca
     7801 tgggcgatga agtccgcggt cagccgctcc agcttggcaa gccccatgta gatcgccacg
     7861 gtctggcgcg gctggatcag cgtcgtccag tccgcgctga tgtcgccatc cttgccatgt
     7921 ccggtcacga aaaggcagga atgggcatgg tcccgatggg tgagcggaat gccggaatag
     7981 gcggcgcagc cagccgctgc cgtaacgccc ggcaccacct gaaacgggat gtgatgcgcc
     8041 gcgagcattt cgatctcctc accgccgcgc ccgaagatga atgggtcgcc gcctttcaga
     8101 cgcagcacgc gcttgccctg tctggccagc gtgacgagca gggaactgat gtcttcctgc
     8161 ggcagggcat gatccgccct ttgcttgccg acgtagatcc gctccgaatc gcggcggacc
     8221 agattgagga tgccgtcgga caccaggcga tcgtagagta cgacgtcgca cttctgcatc
     8281 agccgcaggg cccggaaggt gagcagatcc ggatcgccgg ggccggcgcc gacaaggtag
     8341 acttcgccca tcgctggcgt gtcgcgcgtc ccttctgcct caatcgcggc gttgagtgcg
     8401 gcctcggcct ctttctcctt gccggccagc accatctcgg cgactggccc ttccatgatc
     8461 cgctcccaga agcgccgacg accggcgccg tccgtcacct tgtccttcag cgcgtcgcgg
     8521 aaggtccgga cgaaggcggc aagtcgtcca taggaagcag ggaccgccgc ttcgagccgg
     8581 gccttcagca tgcgccccag gatgggagaa gcgccctccg tgccgatggc gacgacgacg
     8641 gggctgcgat cgaggatggt cggcatggaa aagtcgcaga gcggaggatt gtcggcgaca
     8701 ttgaccggca cgcgtgccgc tttggccagc gcatggacgc gctcggcggt ttcctgatcc
     8761 tccaccgcac aatagaccag cgcgcatgga tcgacatccg aggcctcaat ggcgcgggcc
     8821 tggtggcgga agttggggtg gtccttcacg ccgcggaaat cttcaccgag ttcggcggca
     8881 taggccgtga cggtcgcccc ggcgcgcaac gccatctcgg ccttgcgcga ggcgaccgtg
     8941 ccgccgccca ccaccgcaac cggcttgttg cgcacatcga aatagattgg cagcacgttc
     9001 atgcagtgtc tccgccgggt tggcgtgccg cccagtccgc catcatggtg ccccatcggg
     9061 cggcggtttc ggcgtcgagg tcgatctgtg taatgcggtg gccttcccgc agcaccacgc
     9121 gcagcatgcg catgccgctc tcgaagtcga cctcctcaag gcgaatggca cgccggtacg
     9181 gcgcggcgat ctcgacgatc ggagtccggt tctcgacgct catcgttttt tccccggacg
     9241 ggtctctacc cgatagaacg ccatttcgag ccggttctgc cagtcgcgtg gcgtgtccgc
     9301 gtagtccggc ttcaaatcga agtagagcag gatcatgccg ctccgggccg attccaccac
     9361 cagccgttcc agttcgtaga acgaggtgat ctccggatgc tggatgatca ggtcagagag
     9421 attggccatg tcagtgcctt gcagggcgtc cggtctcgaa gaccgcgttg taggaacgga
     9481 tacgaaagag caggcgtccg ccgtctgcgg tatctgcggc agcattatcg aacgcccgcc
     9541 ggtcatgcag gacattgttg cagatgacgc ccatccccgg ttcgaggcgt agccggtaga
     9601 ccagcggatc attcgccagg atgtcggcaa gcgcttcaag cgcagctgca aggactgggt
     9661 cgtcgcgcca ggtcacgttc cgcttgcgga tggtaaagcg catgatcagc gcgcctgttg
     9721 tcgggtgcgc gacgaagacg ggaccggcca ccgcgccatg ggcgctcacc gtcgcctcct
     9781 cgtgcgccgg gatggtcatg gcttcgggat gcatcagcgc tctgatgtag tcgggattgc
     9841 ggtcgcgcag ccggatatag gcgatctcgg gatcgaacag cccgctctcg ccgccggccg
     9901 cagcgctgcg cacgcaatgc agcgccatcg agcggatgca gcggtccggt ccgcgatagt
     9961 tgtagtagcc gtcggtatgc cagttgatcg cccggttcga gaagggaatg aagccggcgc
    10021 gcaggccttc ttccaccacc tcgatgggga cgattccatc cggcccggac gagcggtgtt
    10081 cctcgaactc ggtcagcccc gcgatttggg cgaaacgggc cagcgcgcgg cgcgcggatt
    10141 cagcatgtcc cgcaagggct ggcgagcggt agaccgccat gttcgcgcgc cggaccatgg
    10201 cgcgcagggc ggccagctcc gattccgtcg gctgcgcgag gtcggcgacg gggatgatca
    10261 ggtcgtccgc ctgcttgggc gcggcgtcga gccgggccgc gcgccaggcg cggtaggcgg
    10321 tctcgtcggc gagcgagaag ggcccggagc ctgctcctgc ggccgtgatc ctggcggtcg
    10381 gcatcgacgt ggacgggatc atgcggggga catcgctcat ggattgtgct ggaggagtga
    10441 cgccgcaagg cgccttcgaa tgacgggggc gggatatggc gcagcaagcc gaaaagccaa
    10501 tgactgctcc ggattggcga gcgaatcgaa tatcttaccg aactctcgat ggccgtgttc
    10561 gcgccgctga ccccaagcga aactgagccg ccgttcgaaa aaatatcggg tcaccccggc
    10621 ccctccctgc aattcatcac ttgcattcat tctaatatac aaatacaaga aaccacaagc
    10681 gggccgtcga gttccgcgac ctggaggaaa gaggccgaag agccttaagc ccctgtcgcc
    10741 aagtcgatcg gccaagccaa accggctgac gtctgcggac gtatggccga gacagcgaaa
    10801 tccagtggtc cgggcgcgaa acggaccaga cggcgaggag gctaccgatg gcggacgaca
    10861 agatggccaa tgagtcgaag ggcggcgact gtggcagtgg gggctgcggt tgcaagacgg
    10921 ctgcgcgtcc gaccccgaat ctcgaccagc ttgaaagcgg cccttggcca agcttcgtca
    10981 cgggcctgaa gcggctgcgc gattccggcg gcaagaaata cgccaccatg atgaacgacc
    11041 tgcttgggca gctcgagcat tcctacacca atcgccttgg ctactggaaa ggcggaacgg
    11101 tctcggtctt cggctatggc ggcggcgtta tcccgcgctt ctcggaagtc gccgacaagt
    11161 ttcctgagtc caaggaattc cacaccctgc gcgtgcaacc gccggccggc tttcactaca
    11221 acaccgatgt gctgcgccag atgtgcgaca tctgggaaga gcatggctcg ggtctgatcg
    11281 ccttccacgg tcagtccggc gacatcatgt tccagggcac ctcgaccgag aacacgcaga
    11341 aggccttcga cgcgctcaac gccatcggct tcgacctggg cggcgctggt ccggcgctgc
    11401 gcacggccat gagctgcgtc ggccatgccc gctgcgagat gagctgctac gacgaggtca
    11461 aggcgcatcg ctccgtcatc aattcgttcc tcgacgagat gcaccgcccg gcgctgccct
    11521 acaagttcaa gttcaagttc tcgggctgcc cgaacgactg cgtcaacgcc atccatcgcg
    11581 ccgatttcgc ggtgatcggc acctggcgcg acgacatgaa ggtcgaccag gaagaggtga
    11641 aggcctttgt cgccaagaag ggccgcaagg agatgatcga tacggtcatc gccatgtgcc
    11701 cgaccagggc cctgtcgctc aacgacgacg atacgcttga tgtggacaac aagtcctgcg
    11761 tgcgctgcat gcactgcctg aacgtcatga ccaaggcgct ctcgccgggc gatgacaggg
    11821 gcgcgacgat cctgatcggc ggcaagcggt cgctcaagat cggcgacctg atgggcatcg
    11881 tcgtggtccc gttcatgccg ctgaacaccg acgaagatca tgagaacctc gtggagttcg
    11941 cgcgaaaggt tctggacttc ttcgccgaga atgcgctcga gcacgagcgt atcggcgaga
    12001 cgatcgaccg tatcgggctg cccttcttcc tggaagcgat cggggtcgag ccggatccga
    12061 acatggtcag gcatccgcgc acgtcgagct acgtccggac ggacaacttc accgaagagg
    12121 cggagaagta tttcgagcgc aaggccgcga aaaacgctgc caagaccgcg gcggaatgag
    12181 cctgctcggg gctggctctg gcgcgctgtc tcgcgccgga atggccggcc gagcgtgaga
    12241 ctgatcgact aggaaggaat ctttcatgag ccaggttcag gccaatgcgc ccgcaaagcc
    12301 tcgtcttccc gacgagcatg gcgtgcccga cccgttccag tacatgcatc ccgtgctgaa
    12361 aaagaactac ggacagtggg cctaccatga tcggccgcgt ccaggtgtcc tgcaccatgt
    12421 gtcgaagacc ggtgacgagg tctggaccgt gcgcgccggc acgcagcgcc agatggatgt
    12481 ctacaccatc cgcaagctct gcgacatcgc cgatcaggtg ggcgagggcc acgtgcgctt
    12541 caccacccgc tcgaacatcg aatacatggt ctcgcggttc gacaaggtgc cggcgctgat
    12601 caaggcgctg aacgaggcgg gcttccccgt cggcggcacc ggcaactcgg tctcgatgat
    12661 ttcccatacg cagggttggc tgcactgcga catcccgggc acggacgctt ccggcgtcgt
    12721 caagagcctg atggacatga tgtacgagga gttcatcaag gaggagatgc cgaaccgcgt
    12781 gcgcatcacc acctcctgct gccagatcaa ctgcggcggc cagggcgaca tcgccatcaa
    12841 cgtccagtac accaagccgc cgaagatcaa ccacgatctc gtcgccaacg tctgcgagcg
    12901 gcctgctgtc gtggcgcgct gcccggtcgc ggccatccgt cccgcgatgg tcaacggcaa
    12961 gccgtcgctg gaagtggacg agcgcaagtg cgtctgctgc ggcgcgtgct atccaccgtg
    13021 tccgccgatg cagatcaatg gcgcggattc gaccaagatc gccatctggg tcggcggcaa
    13081 gcattccaac gcccgttcga agccgacctt ccacaagctc gtggccgcga acctgccgaa
    13141 caacgcgccg cgttggccgg aagtggcgga ggtcgtgcgc aagatcctct acgcctacaa
    13201 ggaggatggc cgttactggg agcgcatggg cgagtggatc gagcgtatcg gctggccgcg
    13261 cttcttcgag atgacgggcc tggccttcac gccgcaccac atcgacacct gggtgggcgc
    13321 gcgccacaac atgaacatgt ccacgcatat ccacttctga agtgggttct ggggagatcg
    13381 ggactgtgaa gttcggaatt gtcgtcaacg aggggcccta tacgcaccag gcctcggaca
    13441 cggcgtatca gttcgccaag gcggccctgg acaagggcca cgaggttgtt cgcgtgttct
    13501 tctaccacga cggggtgaac aacgcgacgc gcctcaccgt tccgccgcag gacgagcgga
    13561 acgtgcagcg caactggtcc gaactcggcg cgaagcacaa ccttgatatg gtcgtgtgca
    13621 tcgccgccgc ccagcgtcgc ggcatcctcg acgaaaacga agccaagcgc cagggcaagg
    13681 atgcgaacaa catcgctccg ggcttccgca tttctggtct cggccagctg atcgaggccg
    13741 gcatcctcgc cgatcgcctt gtggtcttcg gcgactgagg aggcgatgat ggcccaggca
    13801 cagaagaagt tcctctacgt caaccgcaag gcgccgcacg gctcgatcta tgcgctggaa
    13861 tcgctcgaag tcgtgctgat cggcgcggcc ttcgagcagg ccgtcagcct cgccttcctc
    13921 gacgacggcg tttttcagct cgcccggggg cagaacacca agggcatcgg tgccaagaat
    13981 ttctccggca cctatcgggc gctgggcgac tacgacgtgc gcgatctcta tgtcgagcgg
    14041 gaatcgctgg aggagcgcgg cctgagcacg gacgatcttc tcgagatcac gcacgacacc
    14101 gacgatgagg aggatatccc ctcgatcaag atcgtcagcc gcgccgaaat ggccgacatc
    14161 atggcgtcgc aggacgtcat cctgagcttt tgaggaatcg cgatgtccac gctgcacacc
    14221 gtcaacaagt cgccgttcga gcgcaacagc ctgtcgagtt gtcttggcca ctgcaagccg
    14281 ggcgatgcgg tgcttctcat cgaggatgcc gtggtgggcg cgcgcaaggg cggcagtttc
    14341 gcaggccgcc ttgccgaggc cggaaagcaa tgcagcatgt atgcgctggg tcctgatctt
    14401 gccgcccgcg gcctaaagcc cgaggatatg gacgcttgcg tccaggtcgt cgactacggc
    14461 ggtttcgtcg atctggcggc ggagcacgcg cgcacgcagg cgtggctctg aaaaaacttt
    14521 gtttgccgcg gaacgagccg cggatcgtga aggcagaagg agacgagcat gggttatcat
    14581 ttcaacggcc aggacttcgc cgctgacgag gaaggctacc tcaccgacct cagcgtctgg
    14641 agcccggaac tggccggcct catcgcgaag gacgagaaca tcgacatgac cccggaacac
    14701 tgggaggtcg tgaatttcct gcgcgagtat tatgacgagt accagatcgc cccggcgatc
    14761 cgcgtgctca ccaaggcgct cgccaagcgc atgggaccgg acaagtcctc caacaagtat
    14821 ctctacgagc tgttccccta cgggccggcc aagcaggcat gcaagatcgc cggcctgccg
    14881 aagccgacgg gctgcgtctg acgtacgcca ggtgctgccg ctccgcgcgg ccgcgcccaa
    14941 accccagtct gggacactga cgggagccgg cgatgaccgc gctaacgatc gcctatgtca
    15001 tgctctttta cgccgcgacc ctcgtgctcg tcggtggcgt tgggtatcgc atctggcagt
    15061 tcgcgacgac gccggccccg ctgaagattc caaccacgcc ggcgccggtc acgacatccg
    15121 gcgtcgcgtt ccgcctggcg cgggaggttg tcttgttcga aagcctgttc aaggcgaaca
    15181 agtggatctg ggtcttcgga tgggtgttcc acgccgcgct cttgctcgtg ctcttgcgcc
    15241 atgtccgcta ctttcagcat ccggtctggg acgtcatcgc gctggtccag cccgtcggcg
    15301 tctatgccgg cttcgccatg gtcgtcggac tgctcggcct gtgggcgcgc cgctttctcg
    15361 ttgagcgcgt gcgctacatt tcctctccgt ccgatcacct gatgctggca ctcctggtgc
    15421 tgatcggcgc gagcggcctg atgatgaagt ttgtcgccca caccgacatc gtcgcggtga
    15481 aggcgttctt cctcggcctg atgcgcttcg aattccagcc gctgccggct gacccgccgc
    15541 tgctggtgca tctgaccctg gtcgccgcgc tgatgatcgt cttcccgttc tccaagctcc
    15601 tgcatgcgcc gggcgtcttc ttcagcccga cgcggaacca ggccgacaat ccgcgcgagc
    15661 gccgccatct cgcccgctgg gccgcagcct tgggcgatgg gcccacccgg aggcagccgt
    15721 gaccgacgcc cgcgacgcca aggtgccgga ctccccgccg gacttgcccg atcccctgga
    15781 gatgccggcc atcgcctgcg gcgccatggc ccattccagg ccctatcccg cgacaccggc
    15841 catgcagcag gcgctgggct ttgccggcga gctggtcccg gactggaaga acaaggccat
    15901 cgccaagatg ggtgatctgg tcggcaagta ccgctcgctc aaggtctatc tcgattcctg
    15961 cgtcaagtgc ggcgcctgca ccgacaagtg ccattacttt ctcggcaccg gggacccgaa
    16021 gaacatgccg gtcgcgaggc aggacctgtt ccgcaaggtc tatcgccggc acttcacgtt
    16081 ggccggaaaa tatgcaccct ggctcgtcgg cgccgaggat ctgacggaag aggtgctcca
    16141 ggactggaac acctatttcc atcagtgctc tcagtgccgc cggtgttcgg tctattgtcc
    16201 ctacggcatc gacacggccg agatttccat ggccgcgcgc gagatcatgg accacatcgg
    16261 cctgggtcag aagtattcca acgagatcat cggcaaggtc ttcaaggtcg gcaacaatct
    16321 cggcctgccg cccaaggccc tgcgcgcgac gcttgagggc cttgaggagg agatcgagga
    16381 ggacaccggc gccaaggtcc gcctgccact ggatgaagcc ggggccgaca tcctgctggt
    16441 gaccccgagc gcggatttct tcgcctcgcc ccacatggaa ggcctgatcg gctacgccaa
    16501 ggtgttccac gccgccggcg cgaaatggac catttccagc aaggcttccg aggcggcgaa
    16561 cttcggcatg ttcgtcggct cctacgagct gatgcgcgac atctccctgc gcatccgcga
    16621 ggcggctctc gaactgggcg tgaagcgcat cgtcatcggc gaatgcggcc atgcctggcg
    16681 cgtcgcctat tccttcctca acacgctggc cggcccgttc gactttctcg atccgaacta
    16741 cccaaagccg cagcacatct gcgaatacac ctgggacctg atccaggacg gcaagctgac
    16801 cttcgacaag tcgaagaacg atcacatgaa gctgacgttc cacgacagct gcaacgtggc
    16861 gcgcgcctcg cgcatggggc cgacgcccgg cgggcagttc gagatcccgc gcaatatcat
    16921 caaggccgtc tgcaaccact tctacgacat ggcgccggag acgacacgcg aggcgacgtt
    16981 ctgctgcggc ggcggcggtg gattgctgag cgacgagctc atggatctgc gcatcaaggg
    17041 cgcgcagccg cgcatgcggg cgctggagga ggtcgtcaag accaatggcg tcacgcacat
    17101 ggcggcgatc tgcgccatct gcaaggcgca gttctccaag gtcctgccgg aatacaactt
    17161 cgaccgggag atgatcgtct cggtccacca gctggtttcc aacgccatcg tcctggagcg
    17221 cgctccggac cccgacggcg aatgacaacg catgcggggg gcgaccccgg gagagcaccg
    17281 atgaacgaga ccgtggcgcc caagcctcac acattccgca agttcaagga cggcggcaac
    17341 atgtggacgg actggagtga tctccagcag aagatcttca atgcggacaa gtcccacaag
    17401 tgtccgacct atgtgcaccg cacgccgccc tgccagggca gctgcccctc cggacatgac
    17461 atccgcggct ggctggcgat cgcgcggggc caggacaagc cgccggtcgc tggcatgcca
    17521 tggcagcact atgccttcga gcgcatgctg gacgccaacc ccttccctgc cgcgatgggg
    17581 cgcgtctgtc cggcgccctg cgaggacggc tgcaaccgca atgaggtcga tgacttcgtc
    17641 ggcatcaacg gcgtggagca gtacgtcggc gactgggcga tccagaacgg cctgaaagcc
    17701 ccggcggcgg cggctgacac cgggcggaag atcgccgtca tcggcggcgg ccctgcggga
    17761 ctggcggcgg cgttcttcct gcgccgcagc gggcatgccg tcaccatctt cgaagcgcat
    17821 gacaagctcg gcggcatgat gcgtttcggc attcccgggt accgcacgcc gcgcaccatg
    17881 ctggacgcgg agatcggccg catcctcgac atgggcgtgc aggtccgcct caacacccgg
    17941 gtcggctggg atgtgccgat ggctgagctc gaatccgggt tcgacgccat cttctgggcg
    18001 gtcggcgcgc agaagggccg gcctctgccg gtgccgggag ccgatgcgcc gaactgcatg
    18061 accggcgtcg acttcctcga tgccttcaac cagggctggg tgttctccac ggccaggaac
    18121 atcgtcgtcg tcggcggcgg cgacacctcg atcgacgtgg cttcggtggc gcggcgtctg
    18181 ggtcacattt cccaggaaaa cgccaaggac atgtcgacgc acgaactgct cggctacacc
    18241 gcccatgacg tcgccggctc tctgcgccgt gagggcgtca aggcgacgct gacctcgctg
    18301 tttcccatcg agaagatgac ggcggccgag cgcgagcgcg aggacgcgct gcgcgaaggc
    18361 gtcgacatca agggcggcgt catgccgctg gaagtcatca agaacgctga aggccatgcc
    18421 atcgcgctga agatgtgcca gtgcgacatg aagggcatga ccccggttcc gcgcgagggt
    18481 acggaattcg agattccctg cgacatgatc atctcggcca tcggccagat gggtgacctc
    18541 ggcggtgaac tcgccgccct cgacaacggc cgtggcttca tcgccattga tgcggtctac
    18601 aaggtcaaga acaaggacaa gcacttcgct ggcggcgatg tgatccgtcc gcatctgctg
    18661 accaccgcca tcggccacgg ccgcatcgcg gcggagagca tcaacgcctt cctcaagggc
    18721 gaggcgaccg acaagcgccc caaggtggac gtccaccact tcaacctgct ggaggagttg
    18781 cagcagcgca acctcgcccc cgaggcctac gatcaccagc agaagcgcgg cacatccgaa
    18841 gcgaggtacg cgatccataa ctatgaagac cgcggcgcgg cgagcatcat cccgcacaag
    18901 gacctgttca agggtcactt cgccttcatg gaacgggcca agcgcgacga gaaacatgtc
    18961 gaggcggaga acgtcatcgg caatttcgag gagcgcatca tcggcttcac cgaggcgcag
    19021 gccaagcgcg agggcgagcg ctgcatgagc tgcggcatgt gcttcgagtg cgacaattgc
    19081 gtcatctact gcccgcagac ggcggtcggg cgcgtaccga agaaggagcg cgcgctcgga
    19141 cgctacgtct acaccgacta caccaagtgc atcggctgcc acatctgcgc cgatgtctgc
    19201 cccaccggct atatccagat gggccttgga gactgagaat gcgcggcctc tgcattcccc
    19261 tgcgatgggc gcaccggctg gcgctgttgg cggtgctctt tttcgccccg gcactggccg
    19321 gcgcgcagca ggccggacgc acgccgcatc ccgaggtgcc gaaggccagc ggcggccagt
    19381 gcgtggccga gaaggacttc atgcggaaga accacatgaa gctgctcaac caccagcgtg
    19441 acgaaaccgt gcatgacggg ctgaggccgg cggatcggtc gctgaaggcc tgcatcgcct
    19501 gccatgccgt gccgggcgct gacggcaggc cggtgacggt cgccgatccg aagcacttct
    19561 gccgcgcctg ccacgattac gtggcggtca aggtcgactg ctttgaatgc cacgcgtcgc
    19621 gaccggacgc cgccgaccga aaggcggccc tgccgctgcc catgggtcac ggcgatgtcg
    19681 cgcgcatcgg cgactatctg aaggagatcg cgcgatgacc gctccgtcgc aagcctcctg
    19741 ccagtgcggc aagccctccg gcggctgcgc ttccgccagg ccgcaaggga cccgccgcga
    19801 ctttctgaaa gacgcggccg gctttgcgtc cgcgacactg gccccgggcg tcacgctgat
    19861 ggctttcggc gacacggccc aggcgcgtgc gccgaacgag aaggtcagcg cgaaaacccg
    19921 ctggggcatg ctgatcgaca cgaccaagtg cgccgacggc tgcacggcct gcgtcacggc
    19981 ctgttccacc gagaacggct ggaaggacac gggccacccc gaaacggacg cccagtggat
    20041 tcgcaaggtc acgctgcgca atcccaggaa cggcgccacg cgcgcgcttc cggtgatgtg
    20101 ccagcattgc gccagcccac cctgcgtcga tgtctgcccg accggcgcct cgttcaaacg
    20161 cgccgatggc atcgtgctgg tggacaagca cctgtgcatc ggctgccgct attgcatgat
    20221 ggcgtgcccc tacaatgccc gctccttcat ccatgaggcg cttgaggacc agaagcccca
    20281 cgcgccgcgc ggcaagggca cggtcgaatc ctgtacgctg tgcgtgcatc gggtcgatgc
    20341 cggtcgtgcg ccggcctgcg tcgaggcttg cggcagcgcc ggccacaagg cgatgctgtt
    20401 cggtgatctc aacgacccga agagcgagat cgcccgggcc gtcgcggaag caccggtgtc
    20461 gcagatccgc gccgatctgg gcgtcgatcc cggcgtcatg tatcgcggca tctgaattcg
    20521 gaggcggatc atggcccatg tcgggtatcg cgaggcaagc gccggtccgg ggttctgggc
    20581 ggcggtcttc gggctgggcg tgctggtgct cgccggcctg ggctcggcct ggtacatgga
    20641 gcaccacggc cacatcgtca cgggcatgac caaccgcatt gtctggggca tgccgcatgt
    20701 cttcgcgatc ttcctgatcg tcggggcttc cggcgtgctc aatgtggcct cgctcgccag
    20761 cgtcttcggc aagatgccgt acaagccgaa ggcgcgcctg tccggcctgc tcgcgatcgc
    20821 catgctgatg tcgggcctca tcgtgctcgt gctcgatctc ggccggccgg accgcctcac
    20881 cgtcgcgatg acctcctata acttccgctc gatcttcgcc tggaacattt tcctctacac
    20941 cggcttcctg gtgatcgtcg gcgcttatct cgtggtccag atgacgcggc gctggcaggg
    21001 ctggaccggg gcggtcggcg tcaccgcctt tctctggcgc ctgatcctga ccaccggcac
    21061 cggctccatt ttcggctggc tggtggcgcg cgaggcctac aacgcggccg tcatggcgcc
    21121 gctgttcatc gcgatgtcgc tcaacatcgg ccttgccgtg tttatcctcg tgcgcatgag
    21181 cgttgcggcg ggcgagacgc atgacctgac cgacgcgctg ctggagcgga tggcgcggct
    21241 cctcggcatc ttcatcgcgg cggtgctgta tttcaccgca gttcagcacc tgaccaacct
    21301 ctattccgcc tcgcgcatcg gcgttgagcg cttcctcctg gtcgatggcg gcatctatcc
    21361 gatgctgttc tggggcgtgc agatcgtgct cggcagcctg gtgccccttg ccatcgtctt
    21421 ctcgccccgg accgcgcggt cccatccgat gatcgcgctc gccgccgtcc tggtgatgat
    21481 cggcgggctg gcgcagctct atgtcatcat catcggcggg caggcctatc cgctgcagct
    21541 cttcccgggg atggaggttt ccagcagcta ccacgatggc gtggtggcgc ggtacgtccc
    21601 cagcctgccg gagttcggcc ttggcttggg cggcctggcg ctgtccttcc tcatcacctt
    21661 catcggcgtt cgggtgctga agatcctgcc ggtgaatgtc ggggcttccg ccggcgcatg
    21721 aaccggctgt tggtctcggc ggcgcatcgc tcgtcgggga agacgacggt ttcgatcggc
    21781 cttgccgcag ccttcagcgc gcgcggcgag cgcctgcgcc cgttcaagaa ggggccggac
    21841 ttcatcgatc cgctctggct gtcgcgcgct gccgcggtgc cctgctacaa tctggatttc
    21901 aatacccagt ccgccgacga aatccgcgcc ctgttcgcgt gggcgtcgtc gggttgcgat
    21961 ctcgcgctca tcgagggcaa caagggcctg cacgatggca tggacgtgga gggcgcggat
    22021 tcctccgccg cgctggcgaa gctgctggat gcgccggtcc tgctggtcct ggatgtgacc
    22081 gggatcaccc ggggtgtcgc gccgctcctg atggggcagg cggcgttcga tccggacgtg
    22141 aacgtctgcg gcgtcgtgct gaaccgggtg gtctcggcgc gccaggaggc gaagctcagg
    22201 caggccatcg agcgctacag cgccattccc gtgctcggcg ccctgccgcg cgacggggac
    22261 ctccttgtca aggagcgccc cctcgggctg acgacgccgg gcgatgccgg cggtgttgag
    22321 gctcg
//
LOCUS       DAOMXN010000019        21535 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_1468_length_21535_cov_12.370298, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000019 DAOMXN010000000
VERSION     DAOMXN010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 21535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21535
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1468_length_21535_cov_12.370298"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="PK812_03295"
     CDS             <1..315
                     /locus_tag="PK812_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68609.1"
                     /translation="NAGQSPREGGQPERPAAPHGQGPRREDRRDDRRPQAGKPGHDRP
                     QGERSRSDRPWEKRLPRDASRHDRSWKEHARQEKPKSAGPDPDSPFAKLLALKAKLEE
                     KP"
     gene            319..594
                     /locus_tag="PK812_03300"
     CDS             319..594
                     /locus_tag="PK812_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017483738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HRJ68610.1"
                     /translation="MTEGPVERQRLDKWLWHARFGRTRTRSAEIVRSGHVRVNGARIT
                     DPGKGIKAGDVLTLAMPGRTLVIRVVAFAGKRGDSPTAQALYEPVAD"
     gene            complement(630..1091)
                     /locus_tag="PK812_03305"
     CDS             complement(630..1091)
                     /locus_tag="PK812_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012317073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HRJ68611.1"
                     /translation="MIRYALCCEKGHDFESWFRSSADFDEQQQRGLVTCPACGSARVA
                     KQIMAPSLARTDKPESVQPAAPAMQNAQPVALIDDREQHLRAMIRAFREQVLAQSENV
                     GQAFAEEARKIHYGESERRSIYGEASPDAVRELLDEGVECHPLPALPDDRN"
     gene            complement(1099..1356)
                     /gene="grxC"
                     /locus_tag="PK812_03310"
     CDS             complement(1099..1356)
                     /gene="grxC"
                     /locus_tag="PK812_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HRJ68612.1"
                     /translation="MASVTIYTKPYCPYCHDAKALLTSKGVAFEEIDILKRPEERDVM
                     IRKAGGRSTVPQIFIGATHVGGCDDIHDLDARGGLDPMLKG"
     gene            complement(1388..2185)
                     /locus_tag="PK812_03315"
     CDS             complement(1388..2185)
                     /locus_tag="PK812_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HRJ68613.1"
                     /translation="MADETLAATCDAAPPLWRAWLGRFGRKGLDIVFPAHCPGCGAGT
                     GEPGALCGPCWQAMPFVTRPFCERLGLPFATDIGGPLLSPAAIAQPPSFDKARAVALH
                     EGQARDFVHLLKFSDRLDLALPMARLMAGAGREMLVETDILVPVPLHWTRLFGRRYNQ
                     SVLLADAIAGLSGVPVERDALKRRKRTPPQVGLSRNARAVNQAGAFVVPPDMKPRVDG
                     RAVVLIDDVMTSGATLNACAGALRRAGARRIDVLTFTIVAHSGPMPI"
     gene            2264..3172
                     /locus_tag="PK812_03320"
     CDS             2264..3172
                     /locus_tag="PK812_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017482775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRJ68614.1"
                     /translation="MADVPNILDEDLQRRRLERAVKGGPATFLLEHVTSDLGERLSPV
                     LRRFESVLDFFSPLGAGGDALVRRSPDAQTTRLAASVSLACGDGWTIADPAIWDPGSG
                     QYDAIVSLLMLQTLNDIPGALVRLRRALRPDGLFIAALIGGDSLKELRQAFLTAEVEI
                     AGGASPRVAPFADVRAMGHLLQRAGFALPVTDSETLTVRYASALDLMRDLRAMGATNA
                     LSERVRRPLRRAILARAIEAYAERFSDQDGRIRATFEIIWLSGWAPHESQQKPLQPGT
                     GKMRLADALSAVSTARAAIEPAKDKR"
     gene            3169..3621
                     /locus_tag="PK812_03325"
     CDS             3169..3621
                     /locus_tag="PK812_03325"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRJ68615.1"
                     /translation="MTTSRPERPVRVGRAAFRRFIPVATRWADNDVYGHVNNVQYYAY
                     FDTAVNQALIEAGLLDIEQSRVVGLVVETSCTYFESLRFPDRIEIGLAMERLGGSSLT
                     YRIGVFKADAPLCAAVGRFVHVYVDRATQRPVPLTDALRTYAEGLVVA"
     gene            complement(3658..3978)
                     /locus_tag="PK812_03330"
     CDS             complement(3658..3978)
                     /locus_tag="PK812_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68616.1"
                     /translation="MFEQLLKLTGLANMNSTHQLILVSVVVIIVVILGFMTDSLLRSS
                     GFGPVGNAAVIILGIVGGAIIYTLYLQAYRHPYPFLPVAAPIVGGFSLLLVMAILKAL
                     MRRE"
     gene            4153..5025
                     /locus_tag="PK812_03335"
     CDS             4153..5025
                     /locus_tag="PK812_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012708921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRJ68617.1"
                     /translation="MLERLSLALFVLIWSTGWIVAKSVAEAQADPLTFLVLRYLSAGL
                     LIAGFAWIVGASWPSTPRQWMHGLVSGVLLHAIYLGGVWWVIDAGLPAGISALIAALQ
                     PLLTAFLARPLLGERITLTNAIGVATGFAGVLIVLGPKYMHLNPGSASSSFMLVAINV
                     AAMFAVTFGTFYQKRFVAQSDLRSITAVQYLGAFVVTLPVAALLEPMHIPPTLETLYA
                     LLWSVLVLSIGAISLMLVMIRRGAVSKVASLIYLIPPTAAVQAWLMFGEVLTPMQILG
                     MAITALGVFLATRR"
     gene            complement(5038..5568)
                     /gene="hpt"
                     /locus_tag="PK812_03340"
     CDS             complement(5038..5568)
                     /gene="hpt"
                     /locus_tag="PK812_03340"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HRJ68618.1"
                     /translation="MPEKNRIRVLYDEQAIARRNAEIAELIAGTAPERLMVVAILRGS
                     FIFAADLLRALHRAGLSPEVEFMQLSSYRAGTTSSGQVTILKDIEGEVQGRDVLLIDD
                     ILESGRTLAYAKDLLAARGARRVMTAVLIEKPGHRAVQFDADFIGFVSPDLFLVGYGM
                     DVAHKYRELPFVGYIE"
     gene            complement(5661..6347)
                     /locus_tag="PK812_03345"
     CDS             complement(5661..6347)
                     /locus_tag="PK812_03345"
                     /inference="COORDINATES: protein motif:HMM:NF023334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HRJ68619.1"
                     /translation="MTRIQCPTCDATYDLPQDSIGPAGRKVRCAACKTLWVASAEPVA
                     PRLSIMMPEPEQSATPAQRTARPWYERLRAEDRRARAPLGWRLAAGLVVVACLGGAVM
                     LRRPIVAAVPETGAAYAALGLPVNLRGLDLRGVQSSLVNEAGNDMLVVQGEIVNISNQ
                     KKALPRLHFTVRDARGLTLYSWTAQADAKDVLPGQSLQFRRRLASPPSEGQDVLVRFA
                     QRADLVASTR"
     gene            6470..7129
                     /gene="ftsE"
                     /locus_tag="PK812_03350"
     CDS             6470..7129
                     /gene="ftsE"
                     /locus_tag="PK812_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HRJ68620.1"
                     /translation="MVRFENVGLRYGMGPEILKDLSFEIPPHSLQFLTGPSGAGKTTL
                     LRLILLTLRPTRGLITLFGSDTARLDKDGVTALRRKVGVVFQDFRLLDHLTTYENVAL
                     PLRVLGRDEASYRPEVIELLKWVGLGDRMHVQPAILSGGEKQRAAIARATISRPELLL
                     ADEPTGNVDPSLARRLLRLFAEMNRSGTSVVIATHDFGLMDQADARRLVLAEGRLHVY
                     D"
     gene            7116..8075
                     /locus_tag="PK812_03355"
     CDS             7116..8075
                     /locus_tag="PK812_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ68621.1"
                     /translation="MSMTEASIAAGNQRGRLFPWRRDLGTPLLPESSIAGRSLVIVIV
                     IMAFLASITAGTVQLIAEASASWTSSIGREMTIQVRPRGGRDIEADLRRASDIARRTP
                     GIADVRIYSKSEAEKLLEPWLGSGVTLTELPVPRLIVLRLSAGEVLDATRLRAELGVV
                     PTASLDDHRQWLMRLSTMANTLVLVGLVLLALVLTAMALAVAFATRGAMAGNREIIDV
                     LHLVGAEDRFIAREFQRHFLWLGLRGGGIGGALAVLAFATASFLATRWVATPGGDQVE
                     ALFGAFSLGWRGYASIVVIALLVAIITAVISRATVFRNLRGLD"
     gene            8163..8813
                     /locus_tag="PK812_03360"
     CDS             8163..8813
                     /locus_tag="PK812_03360"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HRJ68622.1"
                     /translation="MPDDSTRTGQAAQARRVWSLRFVLRLCLVAGGLAVLAFTAGFLR
                     FVEHVSEQETEFGRAVDGVVALTGGAERIAAALAILDEGRAKRLLITGVNVQTSESDL
                     IRQTGHREMFICCVDIDKRALNTVGNAREVSLWAQSHNYSSLLVVTSGYHMPRAMLEL
                     ARFAPQVQLVAHPVFNEASRPDSWMTNPVVARVLLAEYGKYLGAWLRGLISPRPLS"
     gene            9128..9889
                     /locus_tag="PK812_03365"
     CDS             9128..9889
                     /locus_tag="PK812_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HRJ68623.1"
                     /translation="MILLRSILFAIAFYLNCVFWFILANVGYVVPLSWYYQIAFAWTR
                     TSVWLHKTITGSALEYRGIERIPKGALIVACKHQSAWETVGIFSFFSKPTYILKRELL
                     WIPIFGWHLWALGQIGINRGRGSEALAALKAAAQKAMDEDRQILIFPEGTRRAVGAPP
                     EYKYGVARLYAALNVPVLPIAHNAGLAWPRRKFMKYPYKVICEFLDPIPPGLPPAEFH
                     KLLQERIETATNRLVDEALAEKGQSLSDIQARVES"
     gene            complement(9850..10374)
                     /locus_tag="PK812_03370"
     CDS             complement(9850..10374)
                     /locus_tag="PK812_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003839 - gamma-glutamylcyclotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HRJ68624.1"
                     /translation="MKDHHGDLWVFGYGSLMWRPGFAFVERVPALIKGAHRKLCVYSF
                     NHRGTPERPGLVLGLDRGGSCHGIAFRADPHLHVETIAYLREREQITMVYKEVVRPVF
                     LGDGRVVPALCYIVDRKHRQYASSLKREELLQLVRQGVGKSGANPDYVRNTVAALGEL
                     GIHDSTLAWISERL"
     gene            10453..11499
                     /locus_tag="PK812_03375"
     CDS             10453..11499
                     /locus_tag="PK812_03375"
                     /inference="COORDINATES: protein motif:HMM:NF021423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2125 domain-containing protein"
                     /protein_id="HRJ68625.1"
                     /translation="MPDDVTGMPVRRVSRKGLYIPFLLFALACLGWTGFWFYARGKAI
                     EVMDVWMAREARLGRTWTCPDRGVDGFPFRMRFSCQSPTFVSNEPGRAGNGTLRAVDV
                     TARVVDPRMLIANFSGPLTWTSAAGDSIQIDFGRARASYRGAPGAIDQASLEVDELSA
                     MWRAPGLEPQGLKAKRTEVHVRRSPGASVGTDLALSADAVEFPLLNALAGDNAPGEIR
                     FQTSVAQLAPSPPRDWRQTLEAWRTAGGEARIDQFKVAKGPFGLESKGTVRLDEFRRL
                     EGAIEGQATGLLQVLARAGLASGGGGILGGLLGGIRVQQNDGKPRPLPVNIRFENGRV
                     WFGPIQGPRLAPLY"
     gene            complement(11503..12252)
                     /gene="ubiG"
                     /locus_tag="PK812_03380"
     CDS             complement(11503..12252)
                     /gene="ubiG"
                     /locus_tag="PK812_03380"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HRJ68626.1"
                     /translation="MAATAQHYGVDRDEVSRFARMAADWWDPNGPMKPLHRINPQRLA
                     FLKRALCIHFGRDERAIRALSGLQVLDIGCGAGLLSEPLARMGAEVTGVEPAEETIAV
                     ARSHADESNLSIRYVCGLADDLVSQAETFDVVLAMEVVEHVPDVPAFLRTVSSLVKPG
                     GFFAGSTLNRTLRSYALAIVGAEYVMRWLPVGTHSWEKFVRPREFEEALAGAGLDPLA
                     VEGLVYNPLADTWRLSRDTAVNYFITAAKRG"
     gene            12415..13653
                     /locus_tag="PK812_03385"
     CDS             12415..13653
                     /locus_tag="PK812_03385"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRJ68627.1"
                     /translation="MPRLVMKFGGTSVANVERIKNVARHVKREFDAGHDVAVVVSAMS
                     GKTNELVAWCKDTHPLFDQKEYDTVVASGEQVTSGLLAMALQAIGIPARSWQGWQIPL
                     YTDAAHGSARIAGIDGEGIGKGFAERREVAVISGFQGIHKDSGRITTLGRGGSDTSAV
                     AIAAGLKADRCDIYTDVDGVYTTDPRIVPKARRLDRVAFEEMLEMASLGAKVLQVRSV
                     ELAMVHNVRTYVRSSFDDPAAPHPGTLICSEEEILEQQVVTGIAYSKDEAQISLRGVA
                     DRPGVAAGIFAPLAAGNIVVDMIVQNVSEDGKSTDITFTVPSADYARACDLLGKAKEE
                     IGYRALQGATDVAKISAIGVGMRSHAGVAAKAFRALADRGINIRAITTSEIKFSVLID
                     AAYTELAVRTLHQLYGLDAA"
     gene            13718..15094
                     /locus_tag="PK812_03390"
     CDS             13718..15094
                     /locus_tag="PK812_03390"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRJ68628.1"
                     /translation="MAEKQPQDEPEEPSIVERLVVEAEIAAERMIGWVRLALSLWLFA
                     ALAVSVIAVEILDINLGTIRAMVRQVGLPTQIAFLIVGLLSLWIATPQRWRPWMAYVF
                     VTTDALLIGASIFSALKQTGLDGGFISATPPYAAVPVMFCIGSLRPRPRVQYYATALH
                     STIILAIAFWFGGVTEALDERSIRNLPLFFELPGNTLRFALLVAAGLTIALGAQRTRS
                     LLRKSVRETQRRKALTRFLPAEIAPLVANDRFGGFRQGSRQVASVMFVDIRDSTARAE
                     DLDPQRLSIFISSFRRRVMRAVEANEGIVDKFVGDGALILFGVDRGGDDTDGAAKAVA
                     CARQLQSLIDRWNAKRRFSPPVRVGIGIHTGEVYVGVVGDERRLEFTVLGDSVNVASR
                     LEQLTKDVGMPLLASDATVRAAMEEEHWRLVREEPLRGRSGTVRVMAPVDGPAWQPAR
                     SVADATRN"
     gene            15094..16086
                     /locus_tag="PK812_03395"
     CDS             15094..16086
                     /locus_tag="PK812_03395"
                     /inference="COORDINATES: protein motif:HMM:NF020804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit Rsm22 family protein"
                     /protein_id="HRJ68629.1"
                     /translation="MGTALPSALRVAIEAFGAGVSGREMAHRASAQSTRYRSAGDSTT
                     LETREDAAAYVAVRMPATFAAVAAVLAELTRIRPQFAPLTLLDVACGSGSATFAALTH
                     FPSIEHVHLIDRNPTLLAAAATLADASGMASHRRLSAETAMASVANLPAADLVTLSYA
                     LVEMPEAAIAPLAVRLYAAARQGVVLVEPGSQAGFRRIRLARDVLLSAGARIVAPCTH
                     QGTCLMQSPDWCHFSVRLARSREHLRAKSARVPFEDERFSYLVALRDHPEAQGQAGRI
                     LSPPSLTKAGITLRCCEPAGVIDRHVAARDANFRALRRLSWGDVVELQQAQGRD"
     gene            16194..18464
                     /gene="ptsP"
                     /locus_tag="PK812_03400"
     CDS             16194..18464
                     /gene="ptsP"
                     /locus_tag="PK812_03400"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HRJ68630.1"
                     /translation="MRGALSGPRVLLRRLREVMAEPVSAQERLDRIVVLIAANMVAEV
                     CSVYVLRADGALELYATEGLKREAVHLTTMRSGEGLVGLIARDAQPLSLTDAAAHPAF
                     SYKPETGEEIYASFLGVPILRAGDTLGVLVVQNRASRSYSEEEVEALQTAAMVMAEML
                     ATGELEALARPGTGGIALRRPLALKGRAVADGVGLGHVVLHEPRAVIANLVADDVDKE
                     LARLDAAIESMRASIDDLIDQGSVSHAGEHRDVLETFRMFAHDRGWLRRLREAVLSGL
                     TAEAAVERVQSDVRARLLRQTDPYLRERLHDLDDLANRLLHQLTGTAFTLSAADLPEN
                     AILVARAMGPAALLEYQRARLRGLVLEEGGATSHVAVVARALGIPCVGEVEGISSLVQ
                     QGDAIILDGGTGDVHVRPQPDVEAAYAERARLRAGRQEQYRRLKTEPSVTRDGVKIDL
                     LMNAGLLVDLPNLDDANAAGIGLFRTEILFMVAPTLPRASTQEALYRSILAIAGQKPV
                     TFRTLDIGGDKVLPYLRKVDEENPALGWRAIRIGLDRPALLRTQFRAMLAAAADRELR
                     IMLPMVATTAEFDSARAMLERERERARKLGLPQPKVVKLGAMLEVPSLLFDFDSLLQR
                     VDFLSVGSNDLMQFLFAADRDNPRVAGRFDELSPPFLRAMRRIARKCQEARVPVTLCG
                     EMGGKPLEAMALLAAGFRSLSMSPARIGPVKSMLRSLDLSRATEALERALATADGAPS
                     LKPVLETFASANGVQL"
     gene            18541..19614
                     /gene="prfA"
                     /locus_tag="PK812_03405"
     CDS             18541..19614
                     /gene="prfA"
                     /locus_tag="PK812_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HRJ68631.1"
                     /translation="MLPQDKLDAILARHDEVAAHLSEAGDAALIVRLSKELSEIEDVA
                     AKIREYRAFEVSERDLLAMRDEPGADVDLRAMADEELKLLRARHEALEKDIRLALLPK
                     DAADAGGVILEVRAGTGGDEASLFAGDLFRMYQRYADLKGWKVEVLSLSEGTVGGYKE
                     IIAQIIGEGVYARLKFESGVHRVQRVPVTEAGGRIHTSAATVAVLPEAREADIQVPDS
                     DLRIDTMRSGGAGGQHVNKTESAVRITHIPTGVVVVVQDERSQHKNKAKALAVLKARL
                     YDMERQRLASERAADRRTQVGSGDRSERIRTYNFPQGRLTDHRIGLTLYKLTDIVEGS
                     ALDDVIEPLIADHQAALLAGEVA"
     gene            19611..20486
                     /gene="prmC"
                     /locus_tag="PK812_03410"
     CDS             19611..20486
                     /gene="prmC"
                     /locus_tag="PK812_03410"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HRJ68632.1"
                     /translation="MTVPALHAETGRQAALEVLSVAFGAVGIDAPRREARLVMIAALD
                     IDSAELLARPDAALGPHAHRLVETALRRMRREPLSRILGRREFFGLELAIGPEVLDPR
                     PETEILVEAVLDHAARVGLHARPVRVLDIGTGSGAILAAILAGLPLARGVATDLSQAA
                     LTYAVSNFERLGLAGRVQVLRSDWFASVEGDFDIVVSNPPYIASGAVPGLDAEVRHYD
                     PLLALDGGVDGLAAYRAIASGLASHLSREGFAALELGAGQARAVARAFSDAGFPTLDV
                     RRDLAGIERVLMVHR"
     gene            20753..>21535
                     /locus_tag="PK812_03415"
     CDS             20753..>21535
                     /locus_tag="PK812_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4167 domain-containing protein"
                     /protein_id="HRJ68633.1"
                     /translation="MRPGQNKRMRGRNRRGPNPLTRSYESNGPDVKIRGTAAHIAEKY
                     VQLARDAHASGDPVAAESYLQHAEHYLRLIAAAQQAQFQAQQAAMGIKVDDSEELDDD
                     EFESSVMDRFTFRAPQAPGQQPQDGQQPDVPRPDFPGQPRQDQPRGDFQPRHDQRGGG
                     DRPYGDQRPRHGDFRRDRQFDRNGQQQRFDRPRPQSEEGGDIGLPGFITAPVRVPIDV
                     AGQPGQGDQPDVPEGAMPQDEEGYGRFPMRSRRRRRGRGPRDE"
BASE COUNT         3601 a         6926 c         7112 g         3896 t
ORIGIN      
        1 aacgccggac agtcgccccg ggagggaggc cagccggagc gcccggccgc gccccacggt
       61 caaggtccgc gccgggagga ccggcgtgat gaccggcggc cccaggccgg taagcccggc
      121 catgacagac cgcagggcga acgctcgcgc tcggatcggc cctgggagaa gcgtctgccc
      181 cgggacgcat cgcggcatga ccgctcgtgg aaggagcatg cccgtcagga gaagccgaaa
      241 tccgccgggc cggatcccga ttcgcccttc gccaagctcc tggccttgaa ggccaagctc
      301 gaagagaaac cctgagcggt gacggaaggg ccggtcgagc gccagcgtct cgacaagtgg
      361 ctctggcatg cccggtttgg ccggacgcgg acccgatcgg cggagattgt caggtcaggc
      421 catgtccgag tgaatggcgc gcggatcacc gatcccggca agggcatcaa ggcaggggac
      481 gtcctgacgc ttgccatgcc gggccggacc ctggtgatcc gcgtggtcgc cttcgccggc
      541 aagcgggggg attctccaac ggcgcaggcc ctgtacgaac ctgtcgcgga ctgatccgcg
      601 cgcgccggga accgcgaccg gtcccgccct cagttccggt cgtccggcag cgcggggagc
      661 ggatggcatt cgacgccctc gtccagaagc tccctcaccg cgtccggaga ggcctcgcca
      721 tagatcgagc gccgctcgct ctcgccgtag tggatcttgc gcgcctcctc ggcgaaggcc
      781 tggcccacgt tctccgattg cgcgagcacc tgctcgcgga acgcccggat catggcgcgc
      841 aggtgttgtt cccgatcgtc gataagggcc acgggctggg cgttctgcat cgccggcgcg
      901 gccggctgga cggattccgg cttgtcggtt cgcgccagcg agggcgccat gatctgcttg
      961 gcgacccttg ccgagccgca ggccgggcac gtcaccagac cgcgctgctg ctgttcatca
     1021 aagtccgcgc tggaacggaa ccagctttcg aagtcgtgtc ccttctcgca gcaaagggca
     1081 tagcggatca tggcgggctc accccttgag catcgggtcg aggccgccgc gggcgtcgag
     1141 atcatggatg tcgtcgcacc ccccgacatg cgtcgcgccg atgaagatct gcggaacggt
     1201 tgagcgcccg cccgccttgc ggatcatgac gtcacgttcc tcgggccgct tgaggatgtc
     1261 gatctcctcg aaggcgacgc ccttgctcgt cagcaatgcc ttcgcatcgt ggcaataggg
     1321 gcagtagggt ttggtataga tggtcacgga agccatggtc gcgctccaga ggctgtactg
     1381 aaggccttca tatgggcatc ggaccggaat gtgcaacaat cgtgaaggtc agcacgtcga
     1441 tgcggcgggc tccggcccgg cgcagggccc cggcgcaggc gttcagcgtg gcgcccgacg
     1501 tcatgacatc gtcgatcagc accacggcgc ggccgtcgac gcgcggtttc atgtctggcg
     1561 gtacgacaaa ggcgccggcc tgattgacgg cgcgcgcgtt gcggctcaga ccgacctgcg
     1621 gtggtgtgcg cttgcgacgc ttcagggcat cgcgttcgac cggcacgccg ctcagcccgg
     1681 cgatggcgtc ggccaataga acggactgat tgtatcggcg cccgaaaagg cgtgtccaat
     1741 gcagtggaac cggtacgagg atgtccgttt ctacgagcat ttcgcgaccg gcgccggcca
     1801 tcagccgggc catcggcaag gcgaggtcga gccggtccga gaacttcaag aggtgcacga
     1861 aatcacgcgc ctgaccctca tgcaacgcca ccgcgcgcgc cttgtcgaag ctcggcggct
     1921 gggcaatcgc ggcgggcgag agcagcggac cgccgatgtc ggtcgcgaaa ggcaggccga
     1981 gcctttcgca gaacgggcgc gtcacgaaag gcatggcctg ccagcagggt ccgcaaagtg
     2041 cgcccggttc gccggttccg gcgccgcagc cggggcagtg cgcgggaaaa acgatgtcca
     2101 gccccttgcg gccaaaacgg cccagccagg cgcgccagag cggaggtgca gcgtcgcagg
     2161 tagcggcgag cgtttcatcg gccatgccgg aagtctagca gatggcgtgc gatgcgaaag
     2221 cgtcccgatt gcgcaaagtg cccggctcgc ctatcgaaaa gccatggctg acgtgccgaa
     2281 catcctagac gaggacctcc agcggcgacg cctcgagcgt gccgtgaagg gcgggccggc
     2341 aacgttcttg ctggaacacg tgacatccga cctgggcgag cgcctttcgc cggtcttgcg
     2401 gcgcttcgaa agcgttcttg acttcttttc gcctctcggc gcgggtggcg acgctctcgt
     2461 acggcgtagt cccgatgctc agacaacgcg acttgccgcc agcgtttccc tggcctgcgg
     2521 tgatggctgg accattgcgg acccagcgat ctgggacccg ggcagcgggc agtacgatgc
     2581 catcgtgtcg ctgctgatgc tgcagacgct gaacgacatt ccgggcgcgc ttgtgcggct
     2641 gcggcgcgcc ctgcggcccg acgggctgtt catcgccgcg ctcatcggcg gagactcgtt
     2701 gaaggaactg cgccaggcct ttctgacggc ggaagtcgag atcgccggcg gggcgtcgcc
     2761 gcgtgtcgca cccttcgcgg atgtgcgcgc catgggccat ttgctgcagc gcgccggttt
     2821 cgcgctgccg gtcacggaca gtgaaacatt gaccgtccgc tacgcctctg cactggactt
     2881 gatgcgggat ctgcgcgcca tgggcgcgac caatgcgctg tccgaacgcg tccggcgccc
     2941 gctgaggcgc gccattctgg cccgggcgat cgaggcgtat gcggaacgct tttctgatca
     3001 ggatggccga attcgcgcga cattcgagat catctggctt tcgggctggg ctccccacga
     3061 aagccagcaa aagccgcttc agccgggaac cgggaaaatg cgacttgccg atgcgctcag
     3121 cgccgtatct accgcgcgcg ccgcgattga acccgcgaag gacaagagat gaccacttcg
     3181 cgccctgaaa ggccggttcg tgtgggtcgc gcggcgttcc ggcgcttcat tcccgttgcc
     3241 acgcgttggg cggacaacga tgtctacggt cacgtcaaca atgtgcagta ctatgcctat
     3301 ttcgacacgg cggtgaacca ggcgctcatc gaggcgggac tgctggatat cgagcagagc
     3361 cgcgtcgtcg ggctcgtggt cgagacttca tgcacctact tcgagagtct caggttcccg
     3421 gatcgcatcg agatcggcct tgcgatggaa cgccttggag ggtcgtcgct cacctaccgg
     3481 atcggcgtct tcaaggccga tgcaccgctg tgtgccgccg tcgggcgttt cgtccatgtc
     3541 tatgttgacc gggccacgca aaggccggtc ccgttgaccg atgcgctgcg gacctacgcc
     3601 gaaggcctcg tggtcgccta ggctccagcc ggttcacgcg gtgaatgcgc ctgcgattca
     3661 ctcgcgtctc atgagcgcct tcaggatcgc catgacaagc aggagactga accctccgac
     3721 aattggcgcg gcgacgggca ggaacgggta gggatgccga taggcctgca ggtacagtgt
     3781 gtagatgatg gcgccgccga cgatacccag aattatgact gcagcgttgc caaccggacc
     3841 aaagcccgac gagcgtaaca gcgagtctgt catgaaaccc aggataacca cgatgatcac
     3901 cacgacggat accaggatga gctggtgcgt ggagttcatg ttggcgaggc cggtgagttt
     3961 cagaagctgt tcgaacatgg tggcagccaa tcgcggacga tacgatcatc ccatcacaga
     4021 ttgggaaaga atggcttaag gcaagcggtg acagacctgc ccgcatcggc tgccgcagcg
     4081 gtccaggcat gccctatctt ggcagcatcc cgggggcgcg ctacagccac gttccaaccg
     4141 aaagcacccg ccttgcttga acgcctttcc ctcgcgctct tcgtcctgat ctggtcaacg
     4201 ggatggattg tcgcgaaatc cgtggctgag gcgcaggctg atccgcttac ttttctggtc
     4261 cttcgctacc tgtcggccgg gctcctgatc gccgggtttg cctggattgt cggggcctca
     4321 tggccgtcga cgccaaggca atggatgcat ggactggtca gcggcgttct cctgcatgcg
     4381 atctacctcg gcggggtctg gtgggtgatc gatgccgggc tgccagccgg gatttccgcg
     4441 ctgatcgccg cgcttcagcc gcttttgaca gcctttctgg cgcgcccgct gcttggcgag
     4501 cggatcacac tcacgaacgc catcggtgtc gccaccggct ttgccggcgt gctgatcgtg
     4561 ctcgggccga agtacatgca cctgaatccg gggagcgcga gcagttcatt catgctggtc
     4621 gcgatcaatg tcgcggcgat gttcgccgtc accttcggga cgttctacca gaagcgcttc
     4681 gtggcgcagt cagacctgcg atccatcacg gccgtccagt acctcggcgc gttcgtcgtg
     4741 acattgcccg tcgccgccct gctcgaaccg atgcacattc cgcccaccct cgagacgctc
     4801 tacgcgctgc tctggtccgt gctggtgctg tcgatcggcg cgatctcgct gatgcttgtc
     4861 atgatccggc ggggcgcggt ctcgaaggtg gcctcgctga tctacctcat ccctccgacg
     4921 gctgccgtcc aggcctggct gatgtttggc gaagtgctca cgcccatgca gattctcggg
     4981 atggcaatca ccgcgctcgg cgtgtttctg gcgacgcggc gctgatcggg caggcggcta
     5041 ctcgatgtag ccaacaaaag gaagctcgcg gtacttgtgc gcgacatcca tgccgtagcc
     5101 caccagaaac aggtcgggag aaacaaagcc aatgaagtcc gcgtcgaact gcacggcacg
     5161 atggcccggc ttctcgatta ggaccgctgt catcacgcgc cgcgcgccgc gcgccgccag
     5221 aaggtccttg gcataggcca gcgtgcgccc ggattcgagg atatcgtcga tcagaagcac
     5281 atcgcgtccc tgcacctcgc cctcgatatc cttcaggatc gtcacctgtc cggacgatgt
     5341 cgtgcccgcc cggtagctcg acagctgcat gaactcgact tccggcgaca ggccggcccg
     5401 gtgcaaggcg cgcagcagat cggccgcgaa gatgaagctt ccgcgcagga tcgcgacgac
     5461 catcaggcgt tccggcgcgg ttccggcgat cagctcggca atttccgcat tgcgtcgggc
     5521 gatggcctgc tcgtcataga ggacgcggat gcggttcttc tcgggcatgc ccagactccg
     5581 gttggacggc aagaagcgcc gctatagcat gtccgacgtg gctgcgaagc cgccaggcgc
     5641 gttttcccga tccgttggcc ctagcgcgtg ctggcgacca gatcggcgcg ctgggcgaaa
     5701 cgcacgagga catcctggcc ttcagacggc ggcgaggcca gccgccggcg gaactgcagg
     5761 ctctgacccg gaaggacatc cttggcatcg gcctgcgccg tccagctgta gagcgtcagg
     5821 ccgcgcgcat cgcgaaccgt gaagtggaga cgcggcagag ctttcttctg gttcgagatg
     5881 ttcacgatct cgccctgcac gaccagcatg tcgttgccgg cctcgttgac cagagaggac
     5941 tggacgccgc gcagatccag accgcgcagg ttcaccggca ggccgagagc ggcataggca
     6001 gcccccgttt cgggaaccgc cgcaacgatt ggcctgcgca gcatgaccgc gccgcccagg
     6061 catgccacca ccacaagtcc tgcggccagg cgccagccga gcggcgcacg ggcgcggcgg
     6121 tcctctgccc tcaggcgttc ataccacggc cttgcagtgc gctgcgccgg cgtggccgat
     6181 tgctccggct cgggcatcat gatcgaaagc ctgggtgcga ccggctcggc gctggcaacc
     6241 cacagcgtct tgcacgccgc acagcgcacc ttgcggcctg ccggaccgat cgaatcctgc
     6301 ggcagatcgt aggtcgcatc gcacgtcggg cattgaatgc gcgtcattgg tcctgacggg
     6361 ggaacgaatc agtggcggcc ggggcagtca cttgccccat atccttgcta ttgctggaca
     6421 gacacggtta acggcaggtt aagcgatccg gcgattcgcg ggagcggcta tggtccggtt
     6481 cgaaaatgtt ggcctgcggt acgggatggg gcccgaaatc ctcaaggatc tgagcttcga
     6541 gatccctccg cattccctcc agtttctcac cggcccctcg ggggcgggca agacaacgct
     6601 gttgcgcctc attctcctga cgctgcggcc gacgcgcggg ctgatcacgc tgttcggttc
     6661 cgacaccgcc cggctcgaca aagacggcgt gacggcgctg cggcgcaaag ttggcgtcgt
     6721 cttccaggat tttcgtcttc tcgatcatct cacaacctac gaaaacgttg ccctgccgct
     6781 gcgcgttctg ggtcgggatg aagcctcgta caggccggaa gtcatcgagc tgctgaaatg
     6841 ggtcggcctc ggagaccgga tgcatgtcca gcccgcgatc ctgtcgggtg gcgagaagca
     6901 gcgcgctgcc atcgcgcggg cgacgatttc gcgacctgaa ctgctgctgg cggatgagcc
     6961 cacgggcaat gtcgatcctt ccctcgcaag gcgactgctg cggctgttcg cggaaatgaa
     7021 ccggtccgga acctcggtgg tgattgcgac ccatgatttc ggcctgatgg atcaggccga
     7081 tgctcggcgt ctggtactcg cggaagggcg gctgcatgtc tatgactgaa gcctccatcg
     7141 cggccggtaa ccagcgcggc cggttgtttc cctggcgacg ggacctgggc acgccgctcc
     7201 tgccggaatc gtcgatagcc ggacgctcgc tcgtcatcgt catcgtcatc atggccttcc
     7261 tggcgtcgat cacagccggg acggtgcaac tgatcgcgga agcttcggcc agctggacgt
     7321 cgagcatcgg gcgagagatg accattcagg tgcggccgcg cggcggcagg gacatcgaag
     7381 cggatctgcg gcgcgcatcc gacatcgcca ggcgcacgcc gggcatcgca gatgtccgga
     7441 tttattccaa gagcgaggcc gaaaagctgc tcgaaccctg gcttggaagc ggtgtaacgc
     7501 tcaccgaact tcccgttcca aggctgatcg tcctgcgctt gtccgcaggc gaggtgctgg
     7561 atgcgaccag gttgcgcgcg gaactcggcg tcgtgccgac agccagtctc gacgaccacc
     7621 gacagtggct catgcgcctg tccaccatgg cgaacacgct ggtgctggtg gggctcgtgt
     7681 tgctggcgct ggtgctgacc gcgatggcgc ttgcggtcgc cttcgcgacg cgcggcgcga
     7741 tggccgggaa ccgcgagatc attgacgtcc tccatctcgt cggcgccgag gatcggttca
     7801 tcgcccgcga attccagcgg cacttcctct ggctcggttt gcggggaggg gggataggcg
     7861 gcgcgctcgc ggtgctggcc ttcgcgacgg ccagttttct tgcgacccgc tgggttgcca
     7921 ccccaggcgg cgaccaggtc gaggcgctgt tcggggcatt ttccctcggt tggcgcggtt
     7981 atgcgtcgat cgtcgtcatc gcgctgctgg tcgcgatcat cactgcagtg atctcccgcg
     8041 ccacggtctt tcgcaatctg cgcggtctgg actgatgcgc gatcaggtgc cgcgcgcttg
     8101 cgattcagcg gcgctccatg gcattctcgg agccgctccc gtgagcaacg acggagccgg
     8161 acgtgcctga tgattccacc cgcacggggc aagcagcaca ggcgcggcgc gtctggtccc
     8221 tgcgctttgt gcttcgcctt tgcctcgtcg ccggcggcct ggcggtactg gccttcacgg
     8281 cgggcttcct gcggttcgtg gaacacgttt ctgaacagga gaccgaattc gggcgcgcgg
     8341 tagacggggt cgtcgccctg acggggggcg cagaacggat cgccgcggca ctggcgattc
     8401 ttgacgaggg ccgggccaag cgtctgctga ttacgggcgt caatgtgcag acatccgaat
     8461 ccgacctgat ccgtcagacc gggcaccgcg agatgttcat ttgctgcgtc gatatcgaca
     8521 agcgcgcact caataccgtc ggcaatgccc gagaggtcag cctgtgggca caaagtcaca
     8581 actattccag ccttctcgtc gtcacgtccg ggtatcacat gccgcgcgcg atgctggaac
     8641 tggcgcgctt cgcaccccag gtgcagcttg tcgcccatcc cgtgttcaat gaggcatcaa
     8701 ggccggacag ctggatgacg aatccggtcg tggcgcgcgt tctgctggcg gagtacggca
     8761 agtacctcgg cgcatggctg cgcgggctga tctctccgcg cccgctgagc tagggcgtat
     8821 actcactaac agcgctgcgt ttgacgcggg agattttggc gatcaggcat tttttggctg
     8881 atccgaacca aaccgtccgg attgggagga gccggtggaa aaagctggcc caagatcagc
     8941 gcgccctcgg cagggttctg gagcgcaacg gcctcgaaac cgtctctcgt cccggcaaag
     9001 ctgaacacca gcgccctttc ttgccatcga caatcggcgg attctctgag aggaacggaa
     9061 ccggcaagcc atcgggcttg cgagaagaga cctcacgcta cggctttctg gcctggaacc
     9121 gccgaccatg atcctgctgc gatccatcct tttcgccatc gcattctatc tgaactgcgt
     9181 gttctggttc atcctggcga acgtaggcta cgtcgtgccg ctgtcctggt attatcagat
     9241 cgcctttgcc tggacccgca cgtcggtctg gctgcacaag accatcacgg ggagtgcgct
     9301 ggaatatcgc ggcatcgagc gcatccccaa gggagcgctg atcgttgcct gcaagcatca
     9361 gtccgcctgg gaaaccgtcg gaattttctc tttcttctcg aagcctacct acatcctgaa
     9421 gcgagagctg ctctggattc cgatatttgg ctggcacctg tgggcgctcg gacagatcgg
     9481 catcaatcgc ggccgcggat ccgaggccct ggcggcgctc aaagccgcag ctcagaaggc
     9541 catggatgag gacagacaaa ttctgatttt tccggaagga acgcgccggg ccgtcggtgc
     9601 accgccggag tacaagtacg gcgtggcccg tctgtatgcg gctctcaacg taccggtatt
     9661 gcccatcgcc cacaatgccg gtctggcctg gccgcggcgc aagttcatga agtatcccta
     9721 caaggtcatc tgcgagtttc tcgatcccat cccgccgggc ttgcccccgg cggagtttca
     9781 caagctgctg caggagcgca ttgagaccgc aacgaaccgt ctcgtcgacg aggcgctcgc
     9841 cgagaagggt cagagccttt cggatatcca ggccagagtg gaatcgtgaa tgcccagttc
     9901 cccgagggct gcaacggtgt tgcgcacgta gtccggattg gcgccggact tgccgacgcc
     9961 ctgacgcacc agttgcagga gttcctcccg tttcaggctg gaggcgtact ggcggtgctt
    10021 gcggtcgacg atgtagcaca gggcggggac gacccggccg tcgcccagga agaccggccg
    10081 cacgacttcc ttgtagacca tggtgatctg ttcgcgttcg cgaagatagg cgatggtctc
    10141 gacatggaga tgcggatcgg cgcggaacgc gatgccgtgg caggagccgc cgcggtcgag
    10201 tcccagcacc agacccggtc gctcgggtgt gccgcgatga ttgaaagaat agacgcagag
    10261 cttgcgatgc gcgcccttga tcagggccgg cacccgctcc acaaaggcaa agcccggtcg
    10321 ccacatcagc gagccgtaac cgaacaccca cagatcacca tgatggtcct tcatcgggtg
    10381 atcaattgcg aatgacgaga cgggaatcaa gccggctgag cggaaagcat cgctggcaca
    10441 ggagcaaagt tcatgcctga cgatgtgacc gggatgcctg tgcggcgcgt ttcgcgcaag
    10501 gggctctaca ttcccttcct gctgttcgct ctcgcctgtc ttggctggac cggtttctgg
    10561 ttctacgcac gtgggaaggc gatcgaggtc atggacgtct ggatggctcg cgaggccagg
    10621 cttgggcgca catggacctg ccctgatcgc ggcgtggacg gctttccatt ccgcatgcgc
    10681 ttttcctgtc agtcgccgac cttcgtttcg aatgaaccgg gccgggccgg caacggcacg
    10741 ctccgcgcgg tggacgtgac ggcgcgcgtg gtcgacccgc gcatgctgat cgcgaatttt
    10801 tctggtccgc tgacctggac gtcggccgca ggcgatagca ttcagatcga cttcggccgg
    10861 gctcgcgcca gttaccgtgg cgcgccgggt gccatagacc aggcctcgct cgaagtggac
    10921 gaactttccg ccatgtggcg cgctcccggc ctggagccgc agggcctgaa ggcgaagcgg
    10981 accgaagtgc acgtgcgccg tagcccgggg gcaagtgtcg ggactgacct ggcgctcagt
    11041 gccgacgcgg ttgagtttcc gctgctcaac gcgctggcag gggacaacgc gccgggagaa
    11101 atccgcttcc agacgtctgt ggcgcaactg gctccatcac cgccgcgcga ctggcgccag
    11161 acgctcgaag cctggcgcac ggccggcggc gaagcgcgca tcgatcagtt caaggtcgcc
    11221 aagggaccct tcggtctcga atccaaaggc acagtgcggc tcgacgaatt tcggcgcctg
    11281 gaaggggcca tcgaaggtca ggcaacgggc ctgttgcagg tgctggcgcg cgcggggctt
    11341 gcctccggcg gcggcggcat cctgggcggg ctgctcggcg gaatccgggt ccagcagaac
    11401 gacggcaagc cccgaccgct gcccgtcaat atccggttcg agaacggacg cgtctggttc
    11461 ggcccgatcc aggggccacg cctcgcaccc ctctactgac cctcaacccc gcttcgcggc
    11521 cgtgatgaag tagttcacgg ccgtatcgcg ggacaacctc caggtgtcgg cgagcggatt
    11581 gtagacgagc ccctcgaccg ccagcggatc gaggccggcg ccggcaagcg cttcctcgaa
    11641 ctcgcgcggt ctcacgaact tctcccagga atgggtgccg accggaagcc agcgcatcac
    11701 atattcggcg ccgacgatgg ccagggcgta ggagcgcaag gtgcggttta gggttgaccc
    11761 ggcgaaaaac ccgccgggtt tcaccagcga cgagaccgtg cgcaggaagg ccgggacatc
    11821 gggaacatgc tccaccacct ccatggcgag caccacatcg aacgtctccg cctgcgacac
    11881 gaggtcgtcg gcgaggccgc acacgtagcg gatcgacagg ttgctctcat cggcatggct
    11941 gcgtgcgacg gcaatcgttt cctcggccgg ctccactccg gtcacctcgg cgcccatgcg
    12001 cgcaagaggt tcgctcagga gtccagcccc gcagccgatg tcgagcacct gaaggccgga
    12061 aagagcgcgg atcgcccgtt catcgcggcc gaaatggatg catagcgcgc gtttgagaaa
    12121 agccagtcgc tgcggattga tccggtgcaa gggcttcatg gggccattcg gatcccacca
    12181 atcggcggcc atcctggcga accgggacac ctcgtcgcga tcgacgccgt aatgttgcgc
    12241 tgttgctgcc atggcgcact ccggcgcggt cttgatcctc tgcaacaggc aaacgacttc
    12301 tcaaaccgcg caatcgcctc tatagcggcc gcgcgcgctt gccgccacag gcagtctggc
    12361 tcccgttgac gcaggcgcgc gccacagtcc cgcgaaagaa gcaaagaacg cgtcatgccg
    12421 cgccttgtta tgaaattcgg cggaacctcc gtcgccaacg tcgagcgcat caagaacgtc
    12481 gcgcgtcacg tcaaacgcga gttcgacgcc ggccatgatg tggcggtcgt ggtctcggcc
    12541 atgtccggca agaccaacga actcgtcgcc tggtgcaagg atacccaccc gctgttcgac
    12601 cagaaggagt acgacacggt cgtcgcctcg ggagaacagg tcacctccgg actgctggcg
    12661 atggccctgc aggcgatcgg tattccggcg cgatcctggc aggggtggca gatcccgctc
    12721 tacaccgacg cagcgcatgg ctccgcccga attgccggca tcgatggcga aggcatcggc
    12781 aagggttttg ccgagcgtcg cgaggtcgcg gtgatctccg gcttccaggg catccacaag
    12841 gactccggcc ggattaccac cctgggtcgc ggcggctccg ataccagcgc cgtcgcgatc
    12901 gcggccggtc tcaaggccga ccggtgcgac atctacaccg atgtcgatgg tgtctacacc
    12961 accgatcccc gcatcgtgcc gaaggcgcgg cggttggatc gagtcgcctt cgaggagatg
    13021 ctggaaatgg catccctcgg cgccaaggtg cttcaggtgc gctcggtcga gctcgccatg
    13081 gtccacaacg tgcgcaccta tgtgcgctcc agtttcgacg atccagcggc cccccatccc
    13141 ggcacgctga tctgcagcga ggaggaaatc ttggaacagc aggtcgtcac cggcatcgcc
    13201 tattcgaagg atgaggcgca gatttcgttg cgcggcgtgg cggatcgccc gggcgtcgcg
    13261 gccggcatct ttgcgcccct cgcggccggg aacatcgtcg tcgacatgat tgtccagaac
    13321 gtgtccgaag acggcaagtc caccgacatc accttcaccg tgccctctgc cgactatgcc
    13381 cgcgcctgcg accttctggg caaggccaag gaggagatcg gttatcgcgc cctgcagggt
    13441 gccacggacg tggccaagat ttctgcaatc ggcgttggca tgcgctccca cgcgggcgtg
    13501 gccgccaagg ccttccgggc gcttgccgat cgcggcatca acatccgcgc catcaccacc
    13561 tcggagatca aattctccgt gctgatcgat gcggcctata ccgaactggc agtgcgcacc
    13621 ctgcatcagc tctacggtct cgacgcagcc tgacttcggc cataaattgg cgggaggtgg
    13681 gtgtttgcgc ctgcctgtcg ttcccgcgcg agaccctatg gccgaaaaac aaccgcagga
    13741 cgagccggaa gagccgtcga tcgtcgaacg tctggtcgtc gaagcggaga tcgccgccga
    13801 gcgaatgata ggctgggtgc ggctggccct gtccttgtgg ctgtttgcgg cgctggcagt
    13861 atcggtcatc gctgtcgaga tcctcgacat caaccttggc accattcgcg ccatggttcg
    13921 tcaggtcggc ctgccgacac agatcgcctt cctgatcgta ggcctgctct ccctgtggat
    13981 cgccacgcct cagcggtggc gtccctggat ggcctatgtc ttcgtgacca cggatgcctt
    14041 gctgatcggc gcctcgatat tcagcgctct caagcagacc ggactcgacg gaggctttat
    14101 ttcagcgacg ccgccctatg cggccgtgcc cgtgatgttc tgcatcggca gcctgcgccc
    14161 gcgcccgcgc gttcagtact acgccaccgc cctgcactcg acgatcatac tcgccatcgc
    14221 cttctggttc ggcggggtga cggaggctct cgacgagcgg tcgattcgaa atctgccgtt
    14281 gttttttgaa ttgccgggaa acaccctgcg tttcgcactt ctggtcgccg ccggcctcac
    14341 cattgctctc ggcgcccagc gcacgcggtc gttgttgcgg aaatccgtcc gcgagacgca
    14401 acgccgcaag gccctgaccc ggtttctgcc ggccgagatc gctccccttg tcgccaacga
    14461 ccgctttggc ggttttcgtc agggcagccg gcaggtcgca agcgtgatgt tcgtggatat
    14521 ccgggattcg acggcgcggg cggaggacct cgatcctcag aggctttcca tcttcatttc
    14581 ctccttccgt cgtcgtgtca tgcgggccgt ggaggcaaac gaggggatcg tcgacaagtt
    14641 cgtcggcgat ggcgcgctca tcctgttcgg cgtggaccgc ggtggcgacg acacggacgg
    14701 ggcggcaaag gcggtggcgt gcgcgcgcca attgcagagc ctcatcgacc gctggaacgc
    14761 caagcgtcgg ttttctcctc ccgtgcgtgt cggcataggc atccatacgg gtgaggtcta
    14821 tgtcggcgtc gtcggtgacg aacgacggtt ggagttcacc gttctggggg attccgtgaa
    14881 tgtcgcatcc aggctggagc aactcaccaa ggacgtcggc atgccgcttc tggcatccga
    14941 cgcgaccgta agggctgcga tggaggagga acattggcga ctcgtgcgcg aggagccgct
    15001 gcgtggtcgc agtggcaccg tccgcgtcat ggcgccggtc gacgggccgg cctggcagcc
    15061 cgcgcgttcg gtggcggacg ctacgcgcaa ctgatgggaa ccgccctgcc atccgccttg
    15121 cgggtcgcca tcgaggcatt cggcgccggc gtgtcggggc gcgagatggc ccatcgcgcg
    15181 tcggcccagt cgacccgcta ccggtcagcg ggagactcca cgacgctgga gacgcgggaa
    15241 gacgcggccg cctatgttgc cgtacgcatg ccggcgacct ttgcggcggt tgccgctgtc
    15301 ctggccgagc tcacacgcat cagaccgcaa ttcgccccgc tcacgctgct cgatgtcgcc
    15361 tgtggctccg gttcggcgac cttcgcggcg ctgacccatt ttccctcgat agagcatgtg
    15421 cacctgatcg accgcaatcc aacattgctg gcggcggcgg ctacgctggc cgatgcgtcg
    15481 gggatggcga gccatcgccg cctgagcgcc gaaactgcta tggcgagcgt tgccaacctg
    15541 ccggctgccg acctggtcac cttgtcctat gcgcttgtgg agatgccgga agccgcaatc
    15601 gctccattgg cagtccggct ctatgcggcc gcccgccagg gtgtggttct ggtcgagccg
    15661 gggtcgcagg ccggattccg gcgcatccgg ctcgcacgcg acgtgttgct gtcggcgggc
    15721 gcacggatcg tcgcgccatg cacgcaccag ggtacctgtc tgatgcaatc cccggattgg
    15781 tgccactttt cggtgcggct tgcccgaagt cgcgaacacc tgcgtgcaaa atcggcacga
    15841 gtgccgttcg aggatgaacg cttcagctac ctcgtcgcct tgcgcgatca tccagaagcg
    15901 caagggcaag ccggtcgcat tctctcgcct cccagcctca ccaaggccgg aatcaccctg
    15961 cgctgttgtg agcctgccgg cgtgatcgac cgccatgtcg cggcacgcga cgcgaatttc
    16021 cgcgccttgc gaaggctgtc ctggggcgac gtggttgaac ttcaacaggc gcagggcagg
    16081 gattagttgc tcgtgctctt gcaggactgg catgcgactg gcaatacgac cgttgcgtgc
    16141 cggtgcctgc ccaatccttg tgcaggcatt ggcgatgatg aggtggcgtc gtgattcgtg
    16201 gcgcgctgag cggaccgcgc gtgctcctgc gaaggctcag ggaggtgatg gccgaaccgg
    16261 tcagcgccca ggagaggctt gaccgcatcg tcgtcctgat cgccgcgaac atggtggccg
    16321 aggtgtgctc ggtctatgtg ttgcgcgccg acggggcgct cgaactctat gccacggaag
    16381 gcctgaagcg cgaggcagtt cacctgacca caatgcggtc cggtgaaggc ctcgttggcc
    16441 tgatcgcgcg cgatgcgcag cctctgtcgc tgaccgatgc ggcggcgcat ccggcgtttt
    16501 cctacaagcc ggaaacgggc gaagagatct atgcctcgtt cctgggtgtg cccatcctgc
    16561 gcgccggcga caccctcggc gtcctggtgg tgcagaaccg cgcgagccgg agctattcgg
    16621 aagaggaagt cgaggccctg cagacggccg ccatggtcat ggcggagatg ctggcgaccg
    16681 gcgaactgga agctctcgcc cgccctggca ccggcggcat tgcgcttcgg cggcctcttg
    16741 ccctgaaagg gcgcgccgtg gccgacggcg tgggtcttgg ccatgtcgtc ctgcatgagc
    16801 cgcgtgcagt catcgccaat ctcgtcgccg acgatgtgga caaggagctc gcgcgtcttg
    16861 atgcggccat cgagagcatg cgcgcttcca tcgacgatct catcgaccag ggcagtgtca
    16921 gccatgccgg cgaacatcgg gatgtcctgg agacgtttcg catgttcgcg catgatcgcg
    16981 gctggctgag gcgcctgcgc gaagcggtcc tgtccggcct gaccgcagag gcggcggttg
    17041 agcgggtgca gtcggacgtg cgggcccgat tgctccggca aaccgacccc tacctgcgcg
    17101 agcgcctgca cgatctggat gacctcgcga accggctgct gcatcagctg acgggaacgg
    17161 cgttcaccct gagtgcggcc gacctgccgg agaatgcgat actggttgcc cgggccatgg
    17221 gtccggcggc gctgctggaa taccagcgcg cgcgcctgcg cggcctggtt ctcgaggaag
    17281 gaggggcaac cagccatgtc gccgtggtcg ccagggcgct ggggattccc tgtgttggcg
    17341 aagtcgaggg catttccagc cttgtgcaac aaggcgacgc gattatcctc gatggtggca
    17401 cgggcgatgt gcacgtgcgt ccacagcccg acgtggaggc cgcctacgcc gaacgcgccc
    17461 gactgcgcgc cggccggcaa gagcagtacc gcaggctcaa aacggaaccg agcgtcaccc
    17521 gcgacggcgt gaagatcgac ctgttgatga atgccgggct gctcgtcgac ctaccgaacc
    17581 tggacgatgc gaacgctgcg ggaatcgggc ttttccgtac ggaaatcctg ttcatggtgg
    17641 cgccgaccct gccacgtgca tcgacgcagg aagcgctgta ccgctcgatc ctagccattg
    17701 ccggccagaa gccggtgacc tttcggacgc tcgacatcgg cggcgacaag gtcctgccct
    17761 acctgcgcaa ggttgatgag gagaatccgg cgctgggctg gcgcgccata cgcatcggac
    17821 ttgatcgtcc ggcactgctg cgcacgcagt ttcgcgccat gctggccgcg gctgcggacc
    17881 gtgaactgcg tatcatgctg ccgatggtcg ccaccacggc ggaattcgac tccgcacgcg
    17941 ccatgctgga gcgcgagcgc gagcgtgcgc gcaagctcgg gttgccgcaa cccaaggtcg
    18001 tcaagctcgg cgcgatgctc gaggtaccct cccttctgtt cgatttcgat tcgcttctgc
    18061 agcgcgtgga cttcctgtcg gtcggttcga acgatctgat gcagttcctg tttgccgccg
    18121 accgcgacaa tccccgcgtt gccggccgct tcgacgagtt gagtccgccg ttcctgcggg
    18181 caatgcgtcg tattgcccgc aagtgccaag aggcccgtgt gccggtcacg ctgtgcggag
    18241 aaatgggggg caagcccctg gaagcgatgg cgctgcttgc cgctggcttc cggtcgctct
    18301 cgatgtcgcc tgcccgtatc ggtccggtga agtccatgct gcggtcgctg gacctttccc
    18361 gtgcaacgga ggcgcttgag cgcgccctgg cgacggcgga cggcgcgccg tccctgaaac
    18421 ccgtgctcga gacgttcgcc tccgccaacg gcgttcagtt gtagcgacgc tccaagtatc
    18481 gtgcgccgat ccggcgcgac ttccccgctg catctttccc gcattccaga ccgttccgcc
    18541 atgctgcccc aagacaaact cgatgcaatc ctggcgcgcc acgacgaagt ggccgcgcat
    18601 ctttccgagg ccggcgatgc cgcgctcatc gtgcgtttgt cgaaggaatt gtccgaaatc
    18661 gaggacgtcg ccgccaagat tcgggagtat cgcgccttcg aagtttcgga acgcgatctg
    18721 cttgccatgc gcgacgaacc cggggcggat gtggatcttc gggccatggc cgacgaggaa
    18781 ctgaagctct tgcgggcgcg gcacgaagcg ctggagaagg acatccgcct cgcgctcctg
    18841 ccgaaggatg ctgcggacgc tggtggcgtg atcctcgaag tccgcgccgg gaccggtggc
    18901 gacgaagcgt ccttgttcgc tggtgatctc ttccgcatgt accagcgcta tgcggacctg
    18961 aaaggctgga aggtggaggt cctgtcgctg agcgaaggca cggtcggagg ctacaaggag
    19021 atcatcgcgc agatcatcgg cgaaggggtc tatgcgcggc tgaagttcga gtccggcgtc
    19081 caccgggtgc aacgcgtgcc tgtcacagaa gcgggtggac gaatccacac gtcggccgcg
    19141 accgtcgcgg ttctaccgga ggcgcgggaa gcggatatcc aggtgccgga tagcgacctc
    19201 aggatcgaca cgatgcggtc cgggggggcc ggcggccagc acgtcaacaa gacggaatcg
    19261 gctgtccgca tcacccatat cccgacaggg gtggttgtcg tggttcagga cgagcggtcc
    19321 caacacaaga acaaggccaa ggcgctggcc gtgctcaagg cccggctcta cgacatggaa
    19381 cgtcagcgcc tggccagcga acgcgcggcc gatcggcgca cgcaggttgg ctccggcgac
    19441 cgttcggaac ggattcgaac gtacaacttc ccgcaaggcc gtctgaccga ccaccgcatc
    19501 ggactgacgc tctacaagct gaccgacatc gtcgaaggct cggccctcga cgacgtcatc
    19561 gaaccgttga tcgccgatca ccaggctgcc ctgctggcag gcgaggtcgc gtgacggttc
    19621 cggcactgca tgcggaaacc gggcgacagg cggcgctgga ggtgctgtct gtggcctttg
    19681 gtgcggtcgg cattgacgct cctcgccgcg aggcgcggct cgtgatgatc gcggcactcg
    19741 acatcgattc ggcggaactc ctggccaggc cggacgccgc tttgggtccg catgcccatc
    19801 gactcgtcga aacggcgctg cgccggatga ggcgtgaacc gctgtcgcgc attctcgggc
    19861 ggcgcgaatt cttcggcctc gaactggcga ttgggccgga ggtgcttgat cccaggccgg
    19921 aaacagaaat cctcgtggaa gccgtgctcg atcatgctgc gcgagtaggc ctgcatgccc
    19981 ggccagttcg cgtcctggat atcggtacgg gatcgggcgc gattctggcc gccatcttgg
    20041 ccggcctgcc gctggcaaga ggtgtcgcga ccgacctgtc ccaggccgcc ctgacctatg
    20101 ccgtttcgaa tttcgagcgc cttggccttg ctggacgggt ccaggtgctg cggtcggact
    20161 ggttcgcctc ggtcgagggc gattttgata ttgtggtatc caacccgccg tacatcgctt
    20221 cgggcgcggt gcccgggctt gatgccgagg tgcggcacta tgacccgctg ctcgctctcg
    20281 atggtggagt ggacgggctt gccgcctatc gggcgatcgc ttccggcctt gcgtcgcatc
    20341 tgtcccgcga aggcttcgcg gcgcttgagc ttggcgccgg acaggcgcgc gccgttgccc
    20401 gggcgttttc tgacgcaggc ttcccgacgt tggacgtccg ccgcgatctc gccggtatcg
    20461 aacgtgtcct gatggtgcat cgttgaaacg ccctgcatca atgacttgcc atcccgtgtg
    20521 cggaccgcta tggaccatgt gctccgtgcg cgacgaatgg aatgcctgtc ggattatcag
    20581 gcggacggcc agagcgcatg atgttgaacc ggtatagccg ggccatttcc ctttccagag
    20641 cctgtttgtg tctaactgga tcgatggccg ggccgatggc gagggatcgc cgcaaccggg
    20701 attcatccgg cgggagacgg ccggcggttt tcattggcgc cagagagatt tgatgagacc
    20761 tggacagaac aagcgcatgc ggggccgcaa tcggcgcggc cccaatccgt tgacacggtc
    20821 ttacgagtcc aacgggcctg atgtgaagat tcgcggtacg gccgcacaca tcgccgagaa
    20881 atacgtccag ctcgcgcgcg acgcgcatgc ctccggcgat cccgtcgccg cagagagcta
    20941 tcttcagcat gccgagcact atctgcgact gatcgccgcg gcgcagcagg cccagttcca
    21001 ggcccagcag gctgccatgg gcatcaaggt ggatgattcc gaggaactcg atgacgacga
    21061 gttcgagagc tcggtcatgg atcgcttcac gtttcgtgcg ccgcaggctc cgggccagca
    21121 gccgcaggac ggccagcagc ctgatgtccc gcggcctgat ttccctggcc agccgaggca
    21181 ggatcagccg cgcggcgact tccagccacg gcatgaccaa cgcggcggcg gcgatcgccc
    21241 ttacggcgac cagcgtccgc gtcacggcga tttccgccgc gatcgtcagt tcgaccgcaa
    21301 cggccagcag cagcggtttg accgtccgcg tccgcaatcc gaggaaggcg gcgacatagg
    21361 cctgcccggg ttcatcacgg ctccggtgcg ggtgccgatc gatgtcgccg gacagcccgg
    21421 tcagggcgac cagcccgatg tcccggaggg cgcaatgccg caggacgaag agggttatgg
    21481 gcgctttccg atgcgctcgc gccggcggcg gcgtggccgc gggccgcgcg acgag
//
LOCUS       DAOMXN010000020        21364 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_1482_length_21364_cov_12.288986, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000020 DAOMXN010000000
VERSION     DAOMXN010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 21364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21364
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1482_length_21364_cov_12.288986"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1689
                     /locus_tag="PK812_03420"
     CDS             <1..1689
                     /locus_tag="PK812_03420"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRJ68634.1"
                     /translation="TAAIPGPVTEALHADPGLPEPLDPQAAIAMLPPRAPSVPAVVPA
                     PSLAAPVPTPPPVAPESSPVPFGGLDRDALGRAFAALGRSHGLPASVVQSVSEAYDRL
                     AGIPLWTADGRLTPRADSMLRAIGRADQHGLSPARFARLQAALSGTADRESREAAGTL
                     LAVAYAREARGARINPQQVSRLITVTLTLPEPEEVLARVVAAPDAGLALEAFNPTHPG
                     YLALKRKLAELGSAPAAEAGRPKLTFGPVLTQGMSDARVPIVRDLLGLPPADTAVFDR
                     TLADSLKAFQRERGLRASGRLDRATTAALGGVPETEAAVRNPRADIIANMERWRWLPT
                     ELGADHILVNVPDFRLMLVQNDAPVWQTRVIVGKPETQTPVFSHRMQFLVVNPSWNVP
                     PSIALKEYLPLLQRNPQALSARGLQVVSRGRVVDPASVDWSKVGRTVAIRQPPGERNA
                     LGHIKFMFPNQHAVYLHDTPNRSLFANERRAYSHGCVRVQDPFRLAEAIMGTDWPERR
                     LRSMVGGGERQINLPTDLPVHLAYFTTFVDDAGALQSREDLYGHNRKVKALLGF"
     gene            1927..3621
                     /locus_tag="PK812_03425"
     CDS             1927..3621
                     /locus_tag="PK812_03425"
                     /inference="COORDINATES: protein motif:HMM:NF017736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HRJ68635.1"
                     /translation="MTRNFEIRRVLREPIWRVTTLGVLSLGLLSAGVSGTQDAVANGD
                     TRSLNLYHTHTRESLTVTFRRDGSYDRAGLEKLNWFLRDWRRDEPTNMDPRLFDTLWE
                     TYREVGSSEAVRVVSAYRSPETNSMLRRRSSGVAKHSQHTMGKAMDLHMPDVSMGKVR
                     EIGMRLHRGGVGYYPTAGTPFVHLDVGSVRSWPRMTRPQLERLFPDGKTVHLPADGKP
                     LAGYEVALAEIQQRGGSAISYAEVTSPRRSLWAALFGGEDDEVEVQTTKRGGKAVASR
                     PVSGSRGQQTALASSAGMDQSSVYAVNNAVAVPQVAAAQPVPARPATRPQPVEEKAAE
                     APRPTAVAAVSAPRIEAAKADRDADAPRSVTAPVPPRRPGDLTFAMLAAAAVPVPPVR
                     PADEPQVPAQQLAAVSDPAPKLANVPLPAPRPTAFAAASDTITGAVDRSALPAAIAGG
                     TSPAAPPVTAFAPANVPVPPVRPAIRSPQPQAETAPGKYALKLDTGAMNALMTGVSAS
                     EDKPRSPQKVELPGVRQSLGTVIVAGKFSQSAGAESGRFSGGLVRPLGAGFVKRGE"
     gene            complement(3646..3894)
                     /locus_tag="PK812_03430"
     CDS             complement(3646..3894)
                     /locus_tag="PK812_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2312 domain-containing protein"
                     /protein_id="HRJ68636.1"
                     /translation="MDDMNTATAGHLKAFIERIERLEEEKKSIADDIKDVYAEAKANG
                     FDTKVLRKVVSLRKQDANERKEQDEILALYLSALGMEA"
     gene            4087..5373
                     /locus_tag="PK812_03435"
     CDS             4087..5373
                     /locus_tag="PK812_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HRJ68637.1"
                     /translation="MRAEARRTFEAAVTAADPARFLEGRLPLPAKGRVFLIGAGKAAV
                     GMIQAAESFYLDQCRLDPQRLAGLAVTRHGYGGRTRAIPVIEAGHPVPDIFGIDGTER
                     LLNLADETQRGDIAVVLMSGGGSANLIAPAGEVTLAEKQALTSALLRSGAPIDAINCV
                     RKHVSRVKGGRLAARLAPARVVTLAISDVPGDDPSVIGSGPTVPDMTTLADARAVIER
                     YGIMPPASVADALNDPGNETPKPGDPAFEGATFEIVGRPADALAAAVAQAAALGYAVT
                     DLGADVAGEARDVAARHALMALSLKRAGRKAAILSGGELTVTLRGKGRGGPNQEYALA
                     LALALNGESGIVALACDTDGTDGGQGLASDPAGALVDSRTLTRARSNGVDAAACLADN
                     DSTRFFAAAGDLVITGPTRTNANDLRVLLIDPQAAG"
     gene            5417..5815
                     /locus_tag="PK812_03440"
     CDS             5417..5815
                     /locus_tag="PK812_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1036 domain-containing protein"
                     /protein_id="HRJ68638.1"
                     /translation="MPVSADLRICNTTNNRAGIAIGYQEGQSWVTEGWFNLRPNTCDV
                     ILKGRLNSRYYYVFGLDYDRGGEWSGPNYLCVRDQEFSIRGPQDCFARGYERAGFVEI
                     DTGEQADWTVELGEGGLVGVQPAPALPRTQ"
     gene            5821..7245
                     /gene="pyk"
                     /locus_tag="PK812_03445"
     CDS             5821..7245
                     /gene="pyk"
                     /locus_tag="PK812_03445"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRJ68639.1"
                     /translation="MRRNRRTKLIATLGPASSDDSTVLALFRAGADVFRINMSHSTHE
                     VLRSRVAQIRAIEAQLARPIGILADLQGPKLRVGRFAAGPVTLVNGAAFRLDSDDAPG
                     SERRVKLPHPEILSSIEKGHRLLLDDGKIQLRVSAVGAGYADTVVEVGGPLSDRKGVS
                     VPDTVIPVGALTSKDYSDLEAAIDAGVDWIALSFVQRPEDIAEVRKITRGRALVMAKI
                     EKPQAIERLEEIMEIADALMVARGDLGVEMPLEVVPGLQKRITRLARKYGKPVVVATQ
                     MLESMISAPAPTRAEVSDVATAVFEGADAVMLSAESASGKFPIEAVSTMSRIAESVES
                     DVNYQAVIDGQRAAPEPTGADAIAAAARTVAQTLDLKAIVVWTASGASALRIARERPL
                     TPILMLTTSERTARRMQLVWGIHTMIGRDPTHTADMVRIGTGAARENGFADLGQRIIM
                     VAGVPFGTPGATNMLRIAFVGSGS"
     gene            complement(7253..7879)
                     /locus_tag="PK812_03450"
     CDS             complement(7253..7879)
                     /locus_tag="PK812_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68640.1"
                     /translation="MARLFLFALFLALTLPASQAQSPPRANPAPPADANRSDPKAKPP
                     SPPPAPVTLDSLFEKLAKAKDPQEGRAIAAQIERRWQRSGSDTTDLLMSRAIQLLNGK
                     EHEQALDMLDYIIQLRPGWAEAYNKRATLFFTMDDLDGAMRDIRATLAREPRHFGALA
                     GLGLIFQKMDNPKAAHAAFTQALKLHPQMERLQGMLDRLKLDVEERRL"
     gene            complement(7962..8087)
                     /gene="ykgO"
                     /locus_tag="PK812_03455"
     CDS             complement(7962..8087)
                     /gene="ykgO"
                     /locus_tag="PK812_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012177066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HRJ68641.1"
                     /translation="MKIRNSLKTLMKRHRDNQLVRRRGRVYIINKTQKRFKARQG"
     gene            complement(8248..8904)
                     /locus_tag="PK812_03460"
     CDS             complement(8248..8904)
                     /locus_tag="PK812_03460"
                     /inference="COORDINATES: protein motif:HMM:NF006743.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HRJ68642.1"
                     /translation="MHAPEAPVPPRWLASARAVLSGRRRAILGIAGPPGAGKSSLAAA
                     LVSALGTRAALVPMDGFHLANDELRRLGRAERKGAPDTFDAAGYVALLQRLRNPVPLE
                     TVYAPVFRREIEEAIAGAIPIGPDVALVVTEGNYLLLDDPPWDRVRPLLDACWYVDID
                     ATTRRERLIQRHMRHGRSAEAARAWVLRSDEANASLIEATKDRADAMLRWPPEPSHSP
                     "
     gene            complement(8922..10118)
                     /locus_tag="PK812_03465"
     CDS             complement(8922..10118)
                     /locus_tag="PK812_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HRJ68643.1"
                     /translation="MTVAADRLPLAGIRIVEFSHMVMGPTSGLVLGDLGADVIKVEPM
                     GQGDNTRRLAGSGAGFFATFNRNKRSIQVDLKSGEGIAFVRRLIATADALTENFRAGA
                     LDAMGLGYDALSRDNPRLIYCSLKGFLSGPYEDRAALDEVVQMMGGLAYMTGPPGRPL
                     RAGSSVNDIMGGMFAVIGILSALQERARTGRGQFIKSGLFESNMLLVAQHMMQFAVTG
                     TPARPMPERLAAWAVYDVFDTADGQQVFVGVVSDTQWTDFCAAFDLGDLRNHPDLASN
                     RDRVLRRDLFMPRLRTLFAGLDQETILQRCAAIRLPFAPIMRPQDLFDDPHLNHPGAM
                     LEVSLMDGRKARVPALPLELDGKRLGLHHDLPHAGEHTRAVARDLGLADEEINRLVAN
                     GILGPA"
     gene            complement(10115..11143)
                     /locus_tag="PK812_03470"
     CDS             complement(10115..11143)
                     /locus_tag="PK812_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HRJ68644.1"
                     /translation="MWRQGTAAQAAFGKPDGIKEEQSVFRPGEDGRDVLISECGPRDG
                     LQILTQIMPTAEKCRWIAALHGAGLKEIEVCSFVPAKLMPQMADAAQVVAFARTLPGL
                     TVLALVPNRKGAEAALTAGATKLTIPVSGSEGHSRANVRKSRAEMVDEVAAIVRLRDA
                     LAPDVPVEANISVAFGCTIDGHVDESEVIALAERLVATGVDDTGLSDTTGMGNPAQVR
                     RMFARLIDRIGAKCGAAHLHNTRGLGIANALAAYEAGVRVFDASHCGIGGCPHAPGAS
                     GNVVTEDLVFMFEAMGIRTGVDPEALIAARSIVAGALPGVELHGMLAGAGLPRNHPYP
                     HPPEAPRT"
     gene            complement(11159..11797)
                     /locus_tag="PK812_03475"
     CDS             complement(11159..11797)
                     /locus_tag="PK812_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014763418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HRJ68645.1"
                     /translation="MIVIGLGRFGGAVAKSLTRLGHEVLAIDERDQVVQEFAGQLTHV
                     VQANSTSVEALRQLSCDEFDHAVVAIGSDIEASVLTTLALVELGVPNIWAKAVTERHG
                     KILEKTGAHHVVFPEAAMGERVAHLVTGKMMDFIAFDDGFAIAKTRSPAQYAGKTLAH
                     LELRSRYGVTVVGIKRPHSDFLYAKPESLIELGDILIVSGPTVQVEAFAALT"
     gene            complement(11817..13097)
                     /locus_tag="PK812_03480"
     CDS             complement(11817..13097)
                     /locus_tag="PK812_03480"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HRJ68646.1"
                     /translation="MIIIGIGTGLLMLPLARTDGIGAPFLTALFTATSATTVTGLTVV
                     DTAIYWTGFGQAVIFALCQIGGFGIMSGTTLLMLMVSRKHSVSAQLMVQVEARGIALG
                     DVKLVLFFALRIMLVVQLLVGLFLSLRFLSYDMPAMDAFWHGYFHAGSALTNGGFTSL
                     AGGLTPYMQDPSVILTCAIAVVLGGLGLPVLHDITRHRSRHVAYSLHTKLTLTAAALL
                     LPLGFAATLLWEWDNAATLGPLPWLHKLTNALFHSVMTRSGGLNTFDTAAITQDMMLV
                     TYGLMFIGGGSASMAGGIKATTFLVIILAVWFEVRGTADVAVFKRRISSGVLREALTI
                     FALAFATVATSTLLMRSLTDLPLRHLVFDIISAFANCGLSMGITGSLPASGQCLLIGL
                     MFMGRVGIITVAVGLAMRSSRIQYRYPEERPVIG"
     gene            13318..14061
                     /locus_tag="PK812_03485"
     CDS             13318..14061
                     /locus_tag="PK812_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020665363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRJ68647.1"
                     /translation="MADVSPNAPVAPMATILVVDDDGEILGLVARFLRANAFEVLTAR
                     NGTEMAACLKGAAVDLIVLDLMLPGASGLDLCRELRRNSATPVVMLTAKGDEIDRIIG
                     LEVGADDYLAKPFNPRELLARISAVLRRTQAGRVHPATGARRMLHFSGWQLDTLKREL
                     TDPRGVVVDLSTGEYDLLMAFLDAPQRVLSREFLLDATRIRAPDAFDRSIDVQISRLR
                     RKIEVEQEMIKTIRGAGYIFTPDVTRGQA"
     gene            14058..15422
                     /locus_tag="PK812_03490"
     CDS             14058..15422
                     /locus_tag="PK812_03490"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRJ68648.1"
                     /translation="MTEVGGPIARWRALVDGLAARTILIVLVGIGAIHLVSLWTYRHA
                     LSQQAELASDVRLADQLLGIKRAVMREPQSARESVAHDLSGGPLEAHWSRTEHATPGG
                     PGAGHWDGLAQRLKQAAPELGDDGLIIGANRRAPDDPHLALVSMRLPDDTWLNVSIVT
                     VTPRSVEGHGTLLSTTLMAIGAVLVSMLMVRWLTRPLSTFAEAAKTFSGSGAPVAVPE
                     DGPRELRDLASAFNEMQRRIARLVEDRTQALAAVSHDLKTPITRLRFRAEDITDAALR
                     SAITADLSEMERMLDQTLAYLRGERTDERMQPVDLVAILATVCADISDDGGATTLTGA
                     GSAVISGRRLSLKRAFGNLIENAVKYGYRADVSVTEVGGTVVVAVADAGPGIRPEDRE
                     RALAPFVRLEPSRNQDTGGFGLGLTIAYAIVASHAGTLTLADGVDGGLLVTVCLPRDG
                     GAGA"
     gene            15477..16934
                     /locus_tag="PK812_03495"
     CDS             15477..16934
                     /locus_tag="PK812_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017482515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRJ68649.1"
                     /translation="MMILRFDRWRWIAALFVLCLSSAAPVTAHDGHDHGAPPPPVTAT
                     IAPRADASSADFELVVVAREGRLVITIDTFRGNEPVSDAEIEIDTPHGTQKATAEAEG
                     VYSLAAPWVAKPGTYDLAVTVQANGLVDVLTATLDIPEMAAAPGKGARPAIASRDAAQ
                     RFADGALFVPKDSQRILALRTEFTEERTFNGTFELPGRIIPDPNGVGYVQSSVSGRLM
                     PPPGGFPRLGTPVKAGDVLAHVHPGVGAADVTTQQQQARELDQQITLVRRRLERLQQL
                     QNVVARSQIEDAELELAGLLARRANLDRAPRHTEDLVAPVSGVIAAVQATAGQIADPS
                     MVIFQIIDPNRFWVEALTYDAQAITGTATARLANNRSITLSYRGTGLAERNQSIPIHF
                     GVEGDTKGLRAGQFLTVLATLTEEKSGVAVPRSAVLRGANGQNMVFEHTNAERFVPRE
                     VRIEPLDANRVLIVSGIGKARRIATQGAELLNQIR"
     gene            16934..20077
                     /locus_tag="PK812_03500"
     CDS             16934..20077
                     /locus_tag="PK812_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003602543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRJ68650.1"
                     /translation="MFTFLVTQSLRNRLFVLAVAALLTLYGALTVTRLPVDVFPDLNR
                     PTVTIMTEAEGLAPPEVEQLVTYPIETQMNGLPGVTRVRSVSGVGLSITYVEFDWNTE
                     IFRNRQQIAERLALIQPQLPSGVVPQMGPINSIMGQILLVAVTAPDGVSPMELRETAD
                     FVLRPRLLSIPGVAQVIPIGGEVRQYRVSPNPAALRALKVTFADVEKALQQFGANTGG
                     GFTDQYSREFLIRNIGRTRSLDDLKRMVVATVGGQPVALEQIATVEFAARTKRGEGGY
                     QGRSAVIVSVEKQPGVDTIPLTRQIEAALRDITTTLPQGIRADQVLFRQAGFIEASIN
                     NVRTVLFEAAAVVAIVLFLFLLNTRTTLISLTAIPVSVLVTAIVFHLFGLSINTMTLG
                     GLAIAIGELVDDAVVDVENIFRRLRENREAGNPRTVFEVVVSASQEVRSGIVYATMVI
                     VLVFVPLFALSGIEGRLFAPLGHAYIISILASLVVSITLTPVMAYYLLPGLRRLDHRE
                     SGMIRALKAANAAMLRVAFRYQKTLMAAVAMAVVAAGTFAASLPRAFLPPFNEGTLTI
                     NLLFNPGISLAESHRVGLIAERLLMDVPDVERVGRRTGRAELDEHAEGVHSSEIEVAL
                     KPGGRAKDAIIADIRSQLAILPVATNVGQPISHRLDHMLSGVRAQIALKIYGEDLDTL
                     RALAEEFRGKFAAVPGIVDLQVEKQVRIPQLEIIVDYRRAALYGVQPAAITEQLERLS
                     NGRVVSRVIDGNRRFDVVLRLQDQQRTTRGLGELLIETPMGWVPVRQLADVVETDGPN
                     QILRENGRRRVVVLANADGAADMARVVADIRALLAQASLPPGFSTSLEGTFQAQEDSM
                     RTIGLLSLVSLAMIFAILYSRYRSVVFVAIIMASVPLALIGSVAALWIAGQPLSVASM
                     IGFITLAGIATRNGILKISHIINLAIHEKIPFSPDLVLRGSLDRLTPVLMTALSAGVA
                     LVPLLIDAQAPGKEILHPVAVTIFGGLVTATLLDAVLTPTLVLRYGRAALERIVAEAR
                     EAAESLPRGLAARTETY"
     gene            20093..20461
                     /locus_tag="PK812_03505"
     CDS             20093..20461
                     /locus_tag="PK812_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68651.1"
                     /translation="MRLSIAVAALAASVLAASAHEPRKGPNGGTLVDAGKNHHLELVA
                     KGTEEVVVFLSDADDKPVSAAGFRANAILVVGGKPQRFALEPAEGSRLVGRAPVAVPA
                     GTKGAVQLTGPDGATAQAKF"
     gene            20485..>21364
                     /locus_tag="PK812_03510"
     CDS             20485..>21364
                     /locus_tag="PK812_03510"
                     /inference="COORDINATES: protein motif:HMM:NF019352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HRJ68652.1"
                     /translation="MDRRQFLAGTAALGGLAAAGPAARAQSPLLTVATRQIEVKRRAA
                     TVFGVTGPGGRSGILANEGDRFSGAVFNGTSDPLVMHWHGQVHAPAEQDRARAGGGEL
                     AAGQNDVHDFLLTPGTHWMHSHQLTEQQLLAAPMVTRERSTGDVQDVIVMLHDFSFRT
                     PAELLAGLGGTNAHAAHSGHGAAAPAQGGGHGSHAGMNHGAMHHGAMGHGATGHVRGQ
                     VHANDISYDAYLANDRTLDDPDVVRVERGGKVRLRIINAATATAFFIDTGAIPVDCIA
                     VDGNPCVPHSGRRFPLA"
BASE COUNT         3556 a         6896 c         7161 g         3751 t
ORIGIN      
        1 accgcggcca tcccgggacc ggtcaccgag gccctgcatg ccgatccggg tctgccggaa
       61 ccgctggatc cgcaggccgc catcgccatg ttgccgcccc gggcgcccag cgtgccggct
      121 gtcgttccgg cgccttccct ggcagcgccc gtgccgacac ctcctccggt cgccccggaa
      181 tcgtcgcctg tgcccttcgg cggacttgat cgggatgccc ttggccgcgc cttcgccgcg
      241 ctggggcgca gccatggtct gcctgcctcg gttgtccagt cggtgtcgga ggcctatgat
      301 cggttggccg gcatcccgct ttggaccgca gacggccggt tgacgccacg cgccgactcg
      361 atgttgcgcg cgatcgggcg cgccgatcag catgggctgt cgccagcgcg atttgcgaga
      421 cttcaggcag cattgtccgg aaccgccgat cgcgaatcgc gggaagccgc cggtacgttg
      481 ctggctgtcg cctatgcgcg cgaggcgcgc ggcgcacgca ttaatcccca gcaggtctcc
      541 cggctgatca ccgtgaccct gacgctgccg gagcccgagg aggttcttgc gcgcgttgtc
      601 gcggcgccgg acgccgggct tgcgcttgag gcgttcaatc ccacccaccc gggttatctt
      661 gcgctcaagc gcaagctggc ggagctcggc tcagcgcctg cagccgaggc ggggcgcccg
      721 aagctcacgt tcgggccggt cctgacccag ggcatgagcg atgcacgggt gcccatcgtg
      781 cgggacctgc ttggactgcc gccggccgat acggcggttt tcgacaggac tcttgccgat
      841 agcctgaagg ctttccagcg cgaacgaggc ctgcgcgcga gcggtcggct tgatcgcgcc
      901 acgacggcgg cgcttggcgg cgttcccgag acggaagcag cggtccgcaa tccgcgggcc
      961 gacatcatcg ccaacatgga gcgctggcgc tggctgccga cggaactcgg cgccgaccat
     1021 atcctagtca acgttccgga tttccgcctg atgctcgtcc agaacgatgc gcctgtctgg
     1081 cagacacgcg tcattgtcgg caagccggag acccagacgc cggtgttttc gcacaggatg
     1141 cagttcctcg tggtcaatcc gtcgtggaac gtgccgccct cgatcgccct gaaagagtat
     1201 ctgccgctgc ttcagcgcaa tcctcaggcc ttgtcggcgc gcggactgca ggtggtgagc
     1261 cgcggacggg tggtggatcc ggcctcggtc gactggagca aggtaggccg cacggtcgcc
     1321 atccggcagc cgccgggcga acgcaatgct ctggggcaca tcaagttcat gttcccgaac
     1381 cagcatgcgg tctacctgca tgatacgccg aaccgctcgc tgttcgccaa cgaacggcgg
     1441 gcctacagcc atggctgcgt gcgggtgcag gacccgttcc ggctggcgga agccatcatg
     1501 gggacggatt ggccggagcg acggctgcgc tcgatggttg gcggcggcga gcggcagatc
     1561 aacctgccga ccgacctgcc ggtgcatctg gcctatttca ccacgttcgt cgacgacgcc
     1621 ggagccttgc agtcccgcga ggatttgtat gggcacaacc gcaaggtgaa ggcgctgctg
     1681 ggtttctgag gcacccgcga cggggggtcg cggtaacctt tatggcacaa acagcggccg
     1741 agcgggcgga aggtcggctt gatttcgcca tgatctgccg tcacgaggaa attgaggggc
     1801 cggggttcgg ggaacgggcg tttgcttaac cgaaggttta ccgatccgtc gcgactgtct
     1861 ttgtcgtcgc gcatgatggg ttcgttcggc gcggacgctg tcgattgcga aagcgggcta
     1921 gggcgcgtga ccagaaattt cgaaatcagg cgagtgctgc gagagccgat ctggcgcgtc
     1981 accactcttg gcgtactcag cctcggactc ctgtccgcag gtgtttccgg tacgcaggat
     2041 gccgtcgcca atggcgatac ccgcagcctt aacctctatc atacgcacac gcgggaaagc
     2101 ctcaccgtca cgtttcgccg ggatggatcc tacgaccgcg ccggtctgga aaaactgaac
     2161 tggttcttgc gggactggcg gcgagacgaa ccaaccaaca tggacccgcg gcttttcgac
     2221 accttgtggg agacctatcg ggaagtgggc tcatcggagg ccgtgcgcgt ggtttccgcc
     2281 taccgctcgc cagagacgaa ttccatgctg cgccgccggt cgagcggggt cgccaagcat
     2341 agccagcaca ccatgggaaa ggccatggac ctgcacatgc ccgatgtgag catgggcaag
     2401 gtgcgggaaa tcggcatgcg gctgcaccgt ggcggcgtcg gctactatcc cacagccggc
     2461 acaccgttcg tccacctcga tgtgggcagc gtccgatcct ggccacgcat gacgcgtccg
     2521 cagcttgagc gtcttttccc tgacggcaag acggtgcatc tgccggctga tggcaagcct
     2581 ctggctggct atgaagttgc cctggcggag attcagcagc gcggcggctc ggcgatttcc
     2641 tatgcggagg tcacaagtcc gcgccgcagc ctgtgggctg cgctgttcgg cggggaagac
     2701 gacgaggtcg aggtacagac gacgaaacgc ggcggcaagg ccgtggcttc gagaccggtc
     2761 agcggatcgc gtggccagca aacggctttg gccagcagcg ccgggatgga ccagagcagc
     2821 gtctatgccg tcaacaacgc cgtcgctgtt ccccaggttg ccgctgccca gccggtgccg
     2881 gcccggccgg caacgcggcc gcaacccgtc gaggaaaagg ccgccgaggc acccagaccc
     2941 acagcggtgg cagccgtgtc cgcgcccagg atcgaggcgg cgaaggcgga tagggatgct
     3001 gatgcgccaa ggagtgtcac cgcgccggtg ccgccacgcc gacccgggga cctgactttc
     3061 gccatgctgg ctgcggccgc tgtcccggtg ccgcctgttc gtccggcgga tgagccgcag
     3121 gtgccggcgc aacagcttgc cgccgtttca gacccggcac cgaagcttgc caatgtgccg
     3181 ctgccggcgc cgcgccccac ggccttcgct gcggcttccg acacgattac cggagctgtg
     3241 gatcgctcgg ccctgccggc cgccatcgcc ggaggcacat cgcccgccgc gccgccggtc
     3301 accgccttcg cgccggccaa tgtgccggtg ccgcctgtcc gacccgccat cagaagcccg
     3361 caaccccagg ccgagaccgc tccgggcaaa tacgcgctga agctggacac tggcgcgatg
     3421 aacgcgctga tgaccggcgt gagcgccagc gaggacaagc cccgctcgcc gcagaaggtc
     3481 gaattgccgg gggtgcgcca gagcttgggc acggtcattg tcgccggcaa gttcagtcag
     3541 agcgctggcg ccgaatccgg ccggttctcg ggcggacttg tccgtccgct cggcgccggt
     3601 ttcgtgaagc gtggcgagta gaacgcgtcg gtccgttcgt gcccctcagg cttccatacc
     3661 gagggcgctg aggtagagcg cgagaatctc gtcctgttcc ttgcgctcgt tcgcgtcttg
     3721 cttgcgcagg ctgacgacct tgcggagcac cttggtgtcg aagccgttgg ccttggcctc
     3781 cgcgtagaca tccttgatat cgtcggcgat cgacttcttc tcttcctcca gtcgttctat
     3841 gcgctcgatg aatgccttca aatgtccggc tgtggctgtg ttcatatcgt ccatggcacc
     3901 gtctcgatcc ggttcggaaa tctcatggcc tcgtcgcgcg tggacgcggc gcagtcaagg
     3961 gcctcgggca tgatcgatga tgctgcggtt gcgttttccc cggtcctcgt gtttttaggc
     4021 gccggcctcg gctaggcagc ggaaggtcgg cacactggtg gggcgcgaag ggtgagcagt
     4081 gcgacgatgc gcgcggaagc gcgccgaacg ttcgaggcag cggtcactgc ggccgatccg
     4141 gcgcggttcc tggaaggccg gctgccgctc cctgcgaagg gtcgggtttt ccttatcggc
     4201 gcaggcaagg cagcggtcgg catgatccag gctgccgaat cattctatct tgatcagtgt
     4261 cggctggatc cgcagcggct ggccgggctc gcggtcacgc gccatggtta cggcggccgc
     4321 acgcgcgcca ttcctgtgat cgaggcgggt catcctgttc ccgacatttt cggcatcgac
     4381 ggcaccgagc gcttgttgaa cctggccgat gagacccagc ggggcgatat cgctgtcgtc
     4441 ctgatgtcgg gtggtggctc tgcgaacctg atcgcgcctg cgggagaggt cacccttgcg
     4501 gaaaagcagg ccctgaccag cgcattgctc cgatccggcg ctccgatcga tgcgatcaac
     4561 tgcgtgcgca agcatgtctc gcgagtgaag ggcggacgcc ttgcggctcg ccttgctcca
     4621 gcccgggtcg tgacacttgc catatccgat gtgccgggcg acgatcctag cgtgatcggc
     4681 tcggggccga ccgtgcccga catgacgacg ctggcggatg cgcgcgccgt gatcgagcgt
     4741 tacgggatca tgccgcctgc gagcgttgcc gacgcgctga atgacccggg caatgaaacg
     4801 cccaagcccg gagatccggc tttcgaaggg gctaccttcg agatcgtcgg ccggccggcc
     4861 gatgccctgg ctgcggcggt cgcccaagcg gcggcgctcg gttacgcggt caccgacctt
     4921 ggcgcggatg tcgccggcga agcacgcgat gtggcggccc ggcatgcgct gatggctctg
     4981 tcgctcaagc gggcggggcg gaaagcggcg atcctgtccg gcggggagct cactgtcacg
     5041 ttgcggggca agggacgcgg cggcccgaac caggagtatg cgctggcgct tgcccttgcg
     5101 ctgaacggcg agagcggcat tgtggcgctc gcctgcgata ccgacgggac cgatggaggg
     5161 caggggctgg cgagcgatcc ggccggggca ctggtggatt cccggacgct gacgcgggcc
     5221 cgctcaaacg gggtggacgc ggcggcctgt ctcgccgata acgattcgac gcggttcttt
     5281 gccgccgccg gcgatctcgt gatcacaggg cccacccgga ccaacgccaa tgacctgcgc
     5341 gtcctgctta tcgatcctca agcggccggc tgacggggcc gcggctgtgg ccttgtggct
     5401 gtgtctggcc gctggcgtgc ccgtttccgc agatctgcgc atctgcaaca cgacaaacaa
     5461 tcgtgccggt atcgccatag gctaccagga gggtcagtcc tgggtcacgg aaggctggtt
     5521 caacctgaga cccaacacct gtgacgtgat tttgaagggg cgcctaaact cgcgctatta
     5581 ctacgttttc gggctcgatt acgatcgcgg cggagaatgg agcggcccga attatctttg
     5641 cgtgcgcgat caggagtttt cgatccgtgg tcctcaggat tgcttcgcac gaggctatga
     5701 acgggccgga ttcgtcgaga ttgataccgg cgagcaggcg gattggaccg tggaactggg
     5761 agaaggcgga ttggtgggcg tgcaaccggc ccccgcgttg ccgcgaacac aatgaccgac
     5821 atgcgcagaa atcgccgtac caagcttatc gcaacgctcg gacccgccag ttctgacgat
     5881 tccacggttc tggcgctgtt ccgcgccggg gccgatgtgt ttcggatcaa catgagccat
     5941 tcgacgcacg aggtgctgcg cagccgcgtt gcccagatcc gtgcgatcga ggctcagctg
     6001 gcaaggccca tcggcattct ggctgacctg caggggccga agctgcgcgt cgggcgtttt
     6061 gccgccggtc cggtcactct tgtcaacggc gccgcgttcc gcctcgacag cgacgacgcg
     6121 cccggcagcg aacggcgggt gaagcttccg catccggaga tcctcagctc catcgaaaag
     6181 ggacatcgcc tgttgctgga cgatggcaag atccagttgc gggtttcggc ggtcggagcc
     6241 ggctatgcgg acacggtcgt cgaggttggt gggccgcttt cggaccgaaa gggcgtcagc
     6301 gtgcccgata cggtgattcc tgtcggcgca ttgacctcca aggactactc cgacctggag
     6361 gctgccatcg atgccggcgt ggactggatc gccctgtcct tcgtgcagcg cccggaagac
     6421 attgccgagg tccgcaagat cacacgcggg cgcgccctgg tgatggccaa gatcgagaag
     6481 ccccaggcca tcgagcggct cgaggaaatc atggagatcg cggatgcgct tatggtggcg
     6541 cgcggcgatc ttggcgtgga gatgccgctc gaagtcgttc cgggcctgca gaagcgcatc
     6601 acgcgtctgg cccgcaagta cggcaagccg gtggtcgtgg cgacgcagat gctggaatcg
     6661 atgatcagcg cgcccgcgcc gacgcgcgcc gaggtgtcgg acgtggcaac ggcggtgttc
     6721 gagggcgcgg atgccgtgat gctttcggcg gaaagcgcct ccggaaagtt tccgatcgag
     6781 gcggtttcca ccatgagccg catcgcggaa tcggttgaga gcgatgtcaa ctatcaggcc
     6841 gtcatcgacg gccagcgcgc tgcgccggag ccgaccggcg ctgatgccat cgcggcggcc
     6901 gcgcgcacgg tcgcgcagac gctcgacctg aaggcgatcg tggtgtggac ggcgtcgggc
     6961 gccagcgcgc tgcgcatagc gcgcgagcgg ccgctgacgc cgatcctcat gcttacgacc
     7021 agcgaacgta ccgcccgacg catgcaactg gtctggggta ttcacaccat gatcgggcgc
     7081 gatcccacgc acaccgccga catggtgcgg atcggcaccg gcgcggcgcg ggaaaacggc
     7141 tttgccgacc tgggtcagcg gatcatcatg gtcgcgggcg ttccgtttgg aaccccgggc
     7201 gcaaccaaca tgctgcgcat cgccttcgtc ggctccggga gctgacgacg gatcagagcc
     7261 tgcgttcctc gacgtcgagc ttcagtcggt ccagcatgcc ctgcagccgt tccatctgcg
     7321 gatgcagctt gagtgcctgg gtgaaggcgg catgggctgc cttgggattg tccattttct
     7381 ggaagatcag gcccaggccg gccagcgcgc cgaaatggcg gggttcgcgc gccagggtgg
     7441 cgcggatgtc gcgcatggcg ccgtcaaggt catccatggt gaagaacagc gtggcacgct
     7501 tgttgtaggc ttccgcccag cccgggcgca gctggatgat gtagtccagc atgtcaagcg
     7561 cctgctcgtg ttccttgcca ttcaagagct gaatggcgcg gctcatcaac agatcggtgg
     7621 tgtcgctgcc cgatcgttgc cagcgccgtt cgatctgcgc ggcgatcgcg cgcccctcct
     7681 gcgggtcctt cgccttggcc agcttttcga acaggctgtc cagcgtgacg ggcgccggcg
     7741 ggggtgaggg tggcttcgcc ttcgggtcgc tcctgttggc gtccgccggc ggcgcagggt
     7801 tggcgcgcgg cggcgattgt gcctgggacg ccggaagcgt cagcgctaga aacagggcaa
     7861 acaaaaacaa ccgcgccatc gtcagggtcc tttgaacgga ctctaggcga tggcgcggca
     7921 aatgcaattc acggaaacgg aggcgccgcc tccggcctgc ctcagccctg gcgagccttg
     7981 aagcgcttct gggtcttgtt gatgatgtag acgcggccgc gacggcgcac gagctgattg
     8041 tcgcgatggc gcttcatcag cgttttcaga gagttacgaa tcttcatcac ggcacctgtc
     8101 gaagcggcgc acgcgccgtc atcctgtcga aaactgtatc ccggcgcgtg gaccacgagg
     8161 gatccagcgt tgcccatgac cagacagcca tgaccggaag tgcctctcca tacgaatttc
     8221 gacgcgcttg gcaagggttt tttgccgtca cggggaatgg gatggctccg gcggccagcg
     8281 cagcatcgcg tccgcgcggt cttttgtcgc ctcgatgaga gaggcgttcg cctcgtcgct
     8341 gcgcagaacc caggcgcggg cggcctcggc gctgcgccca tgccgcatgt ggcgttggat
     8401 caaccgctcc cggcgcgttg ttgcatcgat atcgacatac cagcaggcgt cgagcagcgg
     8461 gcgcacccgg tcccacggcg gatcatcaag caacaggtag ttgccttcgg tgacgaccag
     8521 ggcgacatcc ggaccgatcg ggatcgcacc ggcgatggcc tcttcgatct cgcgccggaa
     8581 cacgggcgcg tagacggttt ccagcggcac agggttacgc aggcgttgca gcaaggccac
     8641 gtagccggcc gcatcgaagg tgtcgggcgc acccttgcgc tctgcccgac cgagccggcg
     8701 caactcgtcg ttggcgagat gaaacccatc catgggaacc agtgccgccc gcgttcccaa
     8761 cgcgctgacc agcgccgcgg ccaatgacga ttttccggcc cctggcggtc ctgcgatgcc
     8821 gaggatggcg cggcgcctgc cggacagcac cgcacgggcg cttgccagcc agcggggtgg
     8881 aacgggggct tcgggtgcgt gcatgctgtg atgtcccgat ctcaggccgg accgagaatt
     8941 ccgttcgcga cgagcctgtt gatctcttcg tcggcgaggc ccaggtcacg tgctaccgcg
     9001 cgggtgtgtt cgccggcatg cggcaggtca tggtgcagcc ccagccgttt gccgtcgagt
     9061 tcgagcggca gggcgggcac ccgggccttg cggccatcca tcagcgagac ctccagcatc
     9121 gctcccgggt ggttcaggtg cggatcgtca aacaggtcct gaggtcgcat gatcggtgca
     9181 aagggcagtc ggatcgccgc gcatcgttgc aggatggtct cctggtcgag tccggcgaac
     9241 agcgtgcgca ggcgcggcat gaacaggtcg cgcctgagca cgcggtcccg attgctcgcg
     9301 aggtcgggat ggttccgcag gtcacccagg tcgaaggccg cgcagaaatc cgtccattgc
     9361 gtgtccgaga ccacgccgac gaatacctgc tggccgtcag ccgtgtcgaa gacgtcatag
     9421 accgcccagg cggccagtcg ttccggcatc gggcgggccg gtgttccggt cacggcgaac
     9481 tgcatcatgt gctgggcgac gagcagcatg ttgctttcga acaggccgga cttgatgaat
     9541 tgaccgcgtc cggtacgtgc gcgctcctgc agcgccgaga ggataccgat gacagcgaac
     9601 atgccgccca tgatgtcgtt gacggaggag ccggcgcgca gcggacgacc cggcggtccc
     9661 gtcatgtagg cgaggccgcc catcatctgc accacttcgt ccagcgcggc gcggtcctcg
     9721 tagggaccgc tgaggaaccc cttgagcgaa cagtagatga ggcgcgggtt gtcccgggac
     9781 agcgcgtcgt aaccgaggcc catcgcatcc agcgcgccgg cgcggaagtt ctcggtcagt
     9841 gcgtccgccg tcgcgatgag cctacggacg aaagcgatcc cctcgcccga cttgagatcg
     9901 acctggatgg agcgcttgtt gcgattgaag gtcgcgaaga agccggcgcc ggatccggcc
     9961 agacggcgtg tgttgtcgcc ctggcccatc ggttcgacct tgatgacgtc ggcgccgaga
    10021 tcgccgagca ccaggccgga agtcggcccc atcaccatgt gggagaactc gacaatacgg
    10081 atgcccgcga gcgggagacg atcggctgca acggtcatgt gcgaggcgcc tcgggcgggt
    10141 gcggatacgg gtggttgcgc ggcagcccgg cgcccgcgag catgccgtgc agctcgacgc
    10201 cgggcagcgc cccggcgacg atggagcgcg ctgcgatcag cgcctcggga tcgacgcctg
    10261 tgcggatgcc catcgcctcg aacatgaaca caaggtcctc ggtgaccacg ttgcctgatg
    10321 cacccggcgc gtgcgggcag ccgcctatgc cgcaatggga ggcatcgaac acgcgcactc
    10381 cggcctcgta ggcggccagc gcattggcga tgccgagccc gcgtgtgttg tgcagatgcg
    10441 cggcgccgca cttggccccg atgcggtcga tcagccttgc gaacatgcgc cggacctggg
    10501 ccgggtttcc catgccggtg gtgtccgaca ggccggtatc gtcgacgccc gtcgcgacca
    10561 gtctctcggc gagcgcgatc acctcgctct cgtccacatg tccatcgatg gtgcagccga
    10621 aggcgacgga aatgttggct tccaccggga cgtccggcgc cagtgcatcg cgcagccgaa
    10681 cgatggcggc gacttcgtcc accatttcgg cccggctctt gcggacattg gcgcgggaat
    10741 ggccctcgga gccggagacg gggatcgtca gcttcgtcgc gcccgccgtg agggctgcct
    10801 ccgcgccttt ccggttcggc accagtgcca gcaccgtaag gcccggcagc gttcgggcga
    10861 atgcgaccac ctgtgctgcg tccgccatct gcggcatcag tttcgcgggg acgaacgagc
    10921 agacctcgat ctccttcagc ccggcgccgt gcaacgccgc gatccagcgg catttctcgg
    10981 ctgtcggcat gatctgcgtc aggatctgca agccgtcgcg cggaccgcat tcgctgatca
    11041 gcacatcacg cccatcctct cccggtctga acacggattg ctcctccttg atgccgtccg
    11101 gcttgccgaa ggcggcctga gcggccgttc cttgccgcca catgctgcgc gcggcattct
    11161 aggtcaatgc cgcgaacgct tcgacctgca ccgtcggacc ggagacaatc aggatgtcac
    11221 cgagctcgat taggctttcg ggtttggcat agagaaaatc ggagtggggg cgcttgatgc
    11281 cgacgacggt gaccccgtat cggcttcgca actcgagatg cgcgagggtc ttgccggcgt
    11341 actgcgcggg cgaacgcgtc tttgcgatgg cgaatccgtc atcgaaggcg atgaagtcca
    11401 tcatcttgcc ggtgacgaga tgcgccacgc gttcccccat ggcggcttcc ggaaagacaa
    11461 cgtgatgggc gccagtcttc tccagaatct tgccgtgccg ttccgtcacg gccttggccc
    11521 agatgttggg aacgcccagt tcaacaagcg cgagcgtggt caggacgctg gcctcgatgt
    11581 ccgagccaat cgccacgacc gcatggtcga actcgtcgca tgagagctgc cggagcgctt
    11641 cgacgctggt cgaattggct tgaaccacgt gggtcagttg accggcgaac tcctgcacca
    11701 cctggtccct ctcgtcgatg gcgaggactt catggccgag ccgcgtaagg gacttggcca
    11761 cggcgccgcc aaaccgccca agtccgatga cgatcacgct gtcggatgct ggtttcctag
    11821 ccaatgacgg ggcgctcctc tgggtagcgg tactgaatgc ggctgctgcg catggcaagt
    11881 cccaccgcga ccgtgatgat gccgacgcgc cccatgaaca tcaagccgat cagcaggcac
    11941 tgtccggagg cgggcaggga gcctgtaatg cccatggaca ggccgcagtt ggcgaaggcc
    12001 gagatgatgt caaagaccag atgacgcagc ggaaggtcgg tcaaagagcg catcaggagc
    12061 gtagaggtgg cgactgttgc aaaggccagg gcgaagatcg tcagtgcctc gcgcaggaca
    12121 cccgacgaga ttcgccgctt gaacacggcg acatcggccg tgccacgcac ttcgaaccag
    12181 accgccagaa tgatcaccag gaacgtcgtc gccttgatgc cgccggccat tgaagcggag
    12241 ccgccgccga tgaacatcag tccgtaggtc acgagcatca tgtcttgcgt gatggcggct
    12301 gtgtcaaacg tattgaggcc gcccgagcgg gtcatcaccg agtggaacag cgcgttcgtg
    12361 agcttgtgca gccagggcag ggggccaagc gtcgcggcgt tgtcccactc ccacagtaag
    12421 gtggcggcga aacccagcgg cagcaggagc gcggcggcgg tcagcgtgag cttcgtgtgc
    12481 agcgagtagg cgacatgccg agaacggtgg cgtgtgatgt cgtgcagcac cgggaggccc
    12541 aggccgccga gcacgaccgc gatggcgcac gtcaggatga cggaagggtc ctgcatgtag
    12601 ggggtcaatc cacccgcaag gcttgtgaac cctccgttgg tcaaggctga cccggcgtgg
    12661 aagtagccat gccagaaagc atccatcgcc ggcatgtcgt agctgaggaa gcgaagcgag
    12721 agaaacagtc ccaccagcaa ttggacaaca agcatgatcc tcagcgcaaa gaacagcacc
    12781 agcttgacgt cgccaagcgc gatgccgcgc gcctcgacct gaaccatcag ctgtgcgctg
    12841 acgctgtgtt tgcgcgaaac catcagcatg agcagcgttg ttccgctcat gatgccaaag
    12901 ccgccgatct ggcatagagc gaagatcacg gcctggccga agcccgtcca gtagatggcc
    12961 gtgtcgacga ccgtcagtcc ggtaacggtc gtggcggagg tggccgtgaa cagggcggtc
    13021 aggaatggcg cgccgatgcc gtcggtgcgt gccagcggca gcatcagcaa acctgtgccg
    13081 attccaatga taatcatgaa tgcggcggga accaaccgcg cgtagctgct acccatctcc
    13141 cgcccctgcg accgtcgctt cacgctagcc cgaagtcgaa gccaagggaa cggtgtctgc
    13201 cgtcgttgtc ccgatcgcag gtcgatcagg gttgctgatg ggcgaatcga tgggcactct
    13261 gggcagcgtg cattgtgcca caagggatat tgatgtgact gccggacccc aaatctgatg
    13321 gcggatgttt cccccaacgc gccggtcgct ccgatggcga caatcctcgt tgtcgacgac
    13381 gatggcgaga tcctcggcct tgttgcccgg ttcttgcgcg ccaacgcatt cgaggtgctg
    13441 acagcgcgca acgggactga gatggccgcc tgcctcaagg gtgctgcggt cgacctcatc
    13501 gtgctcgacc tgatgctgcc cggggccagc gggcttgatc tgtgccggga gctgcgccgc
    13561 aactccgcga cacccgtagt catgctgacc gcaaagggcg acgaaatcga ccggatcatc
    13621 ggtctggagg tcggcgcgga tgactatctc gccaagccgt tcaatccgcg tgaactgctg
    13681 gcgcgaatca gcgcggtgtt gcgacgcacc caggccggac gggtccatcc tgcgaccggt
    13741 gcccggcgga tgctgcactt ctccggctgg cagttggaca cgttgaagcg cgaactgacc
    13801 gatccgcgcg gcgtggtcgt tgatctttcg accggcgagt atgacctgct gatggcgttt
    13861 ctcgatgcgc cccagcgcgt gctgtcccgc gagttcctgc tggatgcgac ccgcatccgc
    13921 gcgcccgatg cgttcgatcg gtcgatcgat gtccagatca gccgattgcg gcgcaagatc
    13981 gaggtcgagc aggagatgat caagactata cgtggagccg gctacatatt cacgccggat
    14041 gtgacgcggg ggcaggcgtg accgaggtcg gaggacccat tgcccgctgg cgtgcccttg
    14101 tcgacgggct ggcggcgcgc acgatactga tcgtcctggt tggcatcggt gcgatccacc
    14161 ttgtcagcct ctggacctat cgccatgccc tcagccagca ggcggagctc gcgagcgatg
    14221 tgcgcctcgc cgatcagttg ctgggcatca agcgcgcagt gatgcgcgag ccgcagtctg
    14281 cccgcgaatc cgtggcccac gatctttcag gcgggccgct ggaggcgcac tggagccgga
    14341 ccgagcatgc cactccggga ggccccggcg caggccactg ggatgggctg gcgcagcggc
    14401 tcaagcaggc agcgcccgaa cttggtgacg acggcctgat catcggcgcc aatcgtcggg
    14461 cacctgacga tccgcatctc gcgctggtct cgatgcgtct gccagacgac acatggctca
    14521 atgtctcgat tgtcacggtc acgccgcgtt ccgtggaggg gcatgggacg ctgctgtcca
    14581 cgacgctgat ggccatcggc gccgtgcttg tctcaatgct gatggtgcgt tggctgacgc
    14641 gtccgctctc cacctttgcc gaagccgcga aaaccttttc cgggtcggga gcccctgtcg
    14701 cggttcccga ggatggcccg cgcgagctgc gcgatctggc aagtgcgttc aacgagatgc
    14761 agcggcgcat cgcccggctg gtggaggacc gaacgcaagc gctcgccgcc gtttcccacg
    14821 acctgaagac gccgatcacg cggctgcgtt tccgcgccga ggatatcacg gacgccgcgc
    14881 tgcgcagcgc catcaccgcc gacctctccg aaatggaaag gatgctcgac cagaccctgg
    14941 cttatctcag gggcgagcgc acggacgagc ggatgcagcc ggtcgatctt gtggcgatcc
    15001 tcgcgacggt ctgcgcggac atcagcgacg atggcggggc cacgacgctg acgggcgccg
    15061 gcagcgcggt gatcagcggg cgacggctgt ccctgaaacg cgccttcgga aacctgatcg
    15121 agaatgccgt caaatacggc taccgagccg atgtctcggt gacggaggtc ggcggaaccg
    15181 ttgttgtcgc cgttgccgat gccgggcccg gcattcggcc cgaagatcgg gaacgcgcgc
    15241 ttgcgccctt tgtgcggctc gaaccgtcgc gaaaccagga taccggcggg tttggccttg
    15301 gcctcaccat cgcttatgcc attgtcgcta gccatgccgg cacgctgaca cttgccgatg
    15361 gagtggacgg cgggcttctg gtgacggtgt gtttaccaag ggacggcggc gccggcgcct
    15421 gaaacactct gtaacattcc tgaaatctgc cagacaaaga gccatgccac actggcatga
    15481 tgattcttcg tttcgatcga tggcgctgga ttgctgcgct ctttgtcctt tgcctgtcca
    15541 gcgcggcgcc cgtcacagcc catgatggcc acgatcacgg tgcaccgccc cctccggtta
    15601 ccgccaccat tgcgccgcgc gccgatgcgt cgtccgccga ttttgaactc gtcgtcgtgg
    15661 cgcgagaggg gaggctggta atcacgatcg acacgttccg ggggaacgag ccggtgagcg
    15721 atgccgagat cgagatcgat actccacacg gcacgcagaa ggcgacggcc gaggccgaag
    15781 gcgtctattc cctcgccgcg ccctgggtcg ccaagccggg tacctatgac cttgccgtca
    15841 cggttcaggc caacggactg gtcgatgtgc tcaccgcgac gctggacatc ccggagatgg
    15901 ctgccgcgcc gggcaagggc gctcgcccgg ccatcgccag ccgcgatgcc gcccagcgct
    15961 ttgccgatgg tgcgctgttc gtgcctaagg acagccagcg catcctcgcc ctgcgtaccg
    16021 agttcacgga ggaaaggacc ttcaacggta ccttcgaact gccggggcgg atcattcctg
    16081 accccaacgg cgttggctat gtccagtctt cggtgtcggg acgactgatg ccgccaccgg
    16141 gtggctttcc gcggctcgga acgccggtga aggccggcga tgtcctcgcc cacgtgcatc
    16201 cgggggtcgg cgccgccgat gtgacgacac agcagcagca ggcgcgcgag ctggaccagc
    16261 agatcaccct ggtgcgtcgc agactggaac gtctgcagca gctccagaat gtcgtcgccc
    16321 gttcgcagat cgaagacgcg gaactggagc tcgccggcct gctggcgcgc cgggccaatc
    16381 tcgaccgggc gccccgccat acggaagatc ttgtggcgcc cgtcagcggc gtcatcgccg
    16441 cggttcaggc gacggccggg cagatcgccg atccaagcat ggtgatcttc cagatcatcg
    16501 atcccaaccg cttctgggtg gaggccctca cctacgacgc gcaggcgatc accgggaccg
    16561 cgaccgcacg ccttgccaat aaccggtcga tcacattgag ctatcgtggc acgggactgg
    16621 cggagcgcaa ccagtcgatc cccatccatt tcggtgttga aggtgacacg aagggcctac
    16681 gcgcgggcca gttcctcacg gttctggcca cgctgaccga ggaaaaatcc ggtgtcgccg
    16741 tgccgcgttc ggccgtcctg cgcggggcca acggccagaa catggtgttc gagcatacca
    16801 acgccgaacg cttcgtgccc cgggaagtgc gcatcgagcc gctcgacgcg aaccgcgtgt
    16861 tgatcgtgtc tggcatcggc aaggctaggc ggatcgccac gcagggcgcc gaactgctga
    16921 accagatccg ctgatgttca ccttcctcgt cacccagtcc ctgcgcaacc ggctgttcgt
    16981 gctggccgtc gccgcattgc tgacgctcta cggcgcgctg acggtgacgc ggctgccggt
    17041 cgatgtcttt cccgatctca accggccgac cgtcaccatc atgaccgagg cggaagggct
    17101 tgctccgccc gaggtcgaac aactcgtgac ctacccgatc gagacgcaga tgaacggcct
    17161 gccgggggtc acgcgcgtgc ggtcggtgtc aggcgtcggg ctgtcgatca cctatgtcga
    17221 gttcgactgg aacacggaga tattccgcaa ccgccagcag atcgcagaaa ggctagcgct
    17281 gatccagccg cagttgccga gtggtgtcgt gccgcagatg gggccaatca attccatcat
    17341 ggggcagatc ctgctcgttg cggtgaccgc cccggacggt gtctccccga tggaactgcg
    17401 ggaaacggcg gatttcgttc taaggccgcg tctgctgtcc atcccaggcg tggcgcaagt
    17461 catcccgatc ggtggcgagg tgcgccagta tcgggtctct cccaatccag cagccttgcg
    17521 cgcgctgaag gtgacattcg ccgatgtgga aaaggccctg cagcagttcg gcgccaacac
    17581 cggcggcggg ttcaccgacc agtattcccg cgagttcctg atccgcaaca tcgggcggac
    17641 ccgcagcctc gacgatctca agcgcatggt cgtggccacg gtcggcggtc agcccgtggc
    17701 gctggaacag atcgcgactg tcgagttcgc ggcccgcacc aagcgcggcg aagggggcta
    17761 ccagggtcga tccgccgtta ttgtgtcagt cgagaagcag cccggggtcg acaccattcc
    17821 gctgacccgc cagatcgagg cggcgctccg cgacatcacg acaacgctgc cccagggcat
    17881 ccgggccgat caggtgctgt tccggcaggc cggattcatc gaagcatcga tcaacaacgt
    17941 gcgcaccgtt ctgttcgaag cggccgcggt ggtggcgatc gtgctgttcc tgttcctgct
    18001 gaacacgcgc acgacgttga tctcgcttac cgccatcccg gtgtcggtgc tggtgacggc
    18061 catcgtgttc cacctcttcg gcctgtcgat caacacgatg acgctcggtg gcctggccat
    18121 cgccatcggt gaactcgtgg acgatgccgt ggtggatgtg gagaacatct tccggcgcct
    18181 gcgcgagaac cgggaggccg gcaatccgcg caccgtgttt gaagtcgtcg tttctgccag
    18241 ccaggaagtg cgctccggca tcgtctatgc gacgatggtg atcgtcctcg tgttcgtgcc
    18301 acttttcgcc ctttcaggca tcgaaggacg gctgttcgct ccgctcggcc atgcgtacat
    18361 catttccatt ctcgccagcc ttgtcgtctc gatcacgctg acgccggtga tggcctacta
    18421 ccttctgccg ggtctaaggc ggctcgatca tcgcgagagc ggcatgatcc gcgccctgaa
    18481 ggccgcgaat gccgccatgc tacgggtcgc cttccgttac cagaagacgc tgatggctgc
    18541 ggtcgccatg gccgtagtgg cggccggcac cttcgcggca agcctgcccc gcgctttcct
    18601 gccgccgttc aacgagggga cgctgaccat caacctattg ttcaatcccg gcatttccct
    18661 ggcggaaagc catcgggtcg gtctgatcgc ggagcggctg ctcatggatg tgccggacgt
    18721 ggagcgcgtg gggcgccgga cgggccgcgc tgaactcgac gagcatgccg aaggcgtgca
    18781 ttcctccgag atcgaggtgg cgctgaagcc gggcgggcgg gccaaggacg cgatcatcgc
    18841 cgacatccgc agccagcttg ccatcctgcc cgttgccacc aatgtcggcc agcccatttc
    18901 gcaccggctc gaccatatgc tgtcgggcgt ccgcgcgcag atcgcgctga aaatctatgg
    18961 tgaggacctc gataccctgc gggcgctggc cgaagagttt cgcggcaagt tcgccgcggt
    19021 gcccggcatc gttgatcttc aggtcgagaa gcaggtacga attccgcagt tggaaatcat
    19081 cgtcgactat cgccgcgccg cgctctacgg cgtgcagccc gccgcgatca ccgagcaact
    19141 cgaacgtctc tcaaacgggc gcgttgtatc gcgcgtcatt gatggcaatc gccggttcga
    19201 cgtggtgctg cggcttcagg atcagcagcg caccacgcga ggactgggtg agttgctgat
    19261 cgagaccccg atgggctggg tgccggtgcg ccagctcgcc gacgtggtgg agaccgacgg
    19321 gccgaaccag atcctgcgcg agaatggccg gcgtcgcgtg gtggtgctgg ccaatgccga
    19381 tggcgcggcg gacatggcgc gcgtggttgc cgacattcgt gcgctgctgg cgcaggccag
    19441 cttgccgccc ggcttctcca cgagtctcga aggcacgttc caggcgcagg aagactcgat
    19501 gcggaccatc ggcctcctct ccctggtttc cttggcgatg atcttcgcga tcctctacag
    19561 ccgctaccgg tcggtcgtat tcgtcgccat catcatggca tcggtgccgc tggcgctgat
    19621 cggttcggtc gcggcactct ggattgcggg tcagccgctc tccgttgcct cgatgatcgg
    19681 cttcatcacg ctcgccggaa tcgccacacg caacggcatt ctcaagatca gccacatcat
    19741 caatctggcc atccacgaga agatcccgtt cagtcctgat cttgtcctgc gtggcagcct
    19801 cgaccggctg acgccggtgc tgatgacggc gctgtccgcc ggggtcgcgc tggttccgct
    19861 tctgatcgac gcccaggcgc cgggcaagga gatcctgcat ccggttgccg tcacgatctt
    19921 cggcggcctc gtgacggcga cgctgctgga tgcggtcctg accccgacgc tggtgctgcg
    19981 ctacggccgc gcggcactcg agcggatcgt ggcagaagcc cgtgaggcag ccgaatccct
    20041 gccgcggggc cttgccgccc ggaccgaaac ctactgatca aaggagcctg ccatgcgctt
    20101 gtcgatagcc gtcgccgcac tcgccgccag cgttcttgcc gcttccgccc atgaaccgcg
    20161 caaggggccg aatggcggga cgctggtgga tgccggcaag aaccaccacc tcgagttggt
    20221 tgccaaaggc acggaggaag ttgtggtctt cctgtcggat gcggatgaca agccagtatc
    20281 cgctgccggg ttcagggcca atgccatcct ggtcgtcggc ggcaagccgc aacgctttgc
    20341 cctggagccg gcggaaggat cgcgcctcgt cggcagggcg cctgtggcgg tgcccgcagg
    20401 cacgaaaggc gcggtgcagc tgaccggccc ggacggagcc accgcgcagg cgaaattctg
    20461 aacactccag agaggagctt tgccatggat cgccgccagt ttcttgcagg aaccgccgcc
    20521 ctcggtggcc ttgccgcagc cgggccggcc gcccgcgcgc agtcaccgct cctgaccgtg
    20581 gccacgcgtc agatcgaggt gaaacgccgc gccgccacgg tcttcggcgt gaccggaccc
    20641 ggcggtcgca gcggcattct tgccaatgag ggtgaccggt tcagcggggc cgtattcaac
    20701 gggaccagcg atccgctggt catgcactgg cacggacagg tgcatgcgcc ggcggaacag
    20761 gaccgcgccc gcgccggcgg cggagaactg gcggcgggcc agaacgacgt ccatgatttt
    20821 ctgctgacgc ccggcacgca ctggatgcac tcgcaccagt tgaccgaaca gcagcttctg
    20881 gccgcgccga tggtgacgcg cgagagatca accggggacg tgcaggacgt gatcgtgatg
    20941 ctgcacgatt tttcgttccg cacgccggcg gaactgctgg cggggctcgg agggaccaac
    21001 gcgcatgccg cgcattccgg gcatggcgcg gcagctccgg cgcagggggg cggacacggc
    21061 tcgcacgccg gcatgaacca cggcgcgatg caccacgggg ccatgggaca tggcgcgacg
    21121 gggcacgtcc gcgggcaggt ccatgccaac gacatcagct atgacgccta tcttgccaat
    21181 gaccgaacac tggacgatcc agatgtcgtc cgggtcgagc gcggtggcaa ggtgcgcctt
    21241 cgcatcatca acgcagcgac cgctaccgca ttcttcatcg acaccggagc cattccggtt
    21301 gattgcatcg cggtcgacgg caacccctgc gttccgcact ccggccgccg ctttccgctg
    21361 gcgc
//
LOCUS       DAOMXN010000021        21243 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_1499_length_21243_cov_11.179866, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000021 DAOMXN010000000
VERSION     DAOMXN010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 21243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21243
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1499_length_21243_cov_11.179866"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="PK812_03515"
     CDS             <1..275
                     /locus_tag="PK812_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRJ68653.1"
                     /translation="FAGYARGALVFVLSDGLERGDPAAMIRGVRRLAARAFRIDWFSP
                     LAGDAEYRPETEALSAIRPLLASLGDASSTQRICKSILSIGTARAA"
     gene            272..>379
                     /locus_tag="PK812_03520"
     CDS             272..>379
                     /locus_tag="PK812_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRJ68654.1"
                     /translation="MTELVDSHFHIWNQADLPWLVGPMQPRIFGPYEGIR"
     assembly_gap    380..389
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <390..1181
                     /locus_tag="PK812_03525"
     CDS             <390..1181
                     /locus_tag="PK812_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRJ68655.1"
                     /translation="EPIRRDYLIGEYLADCRTAGITQAVYVQANWAPERAMDEVDYVS
                     RASTESGFPIMIVAHADMLADDARPALGQLARNRRVRGVRMQLHWHENPLYRFAKSPD
                     LARDPRLLRNVAALAEFGWSFDLQVFAHQMAGAADLARHCPDVTFVLQHAGMLEDTAP
                     AGVTAWRDGMRRLADCPNVVSKLSGLGTFLRANDPAHIAFVVDETLALFGAERCLFGS
                     NFPIEKLWTDYGALIGAYRAAVPAEAHEHIFTRTACRVYRLSEKT"
     gene            1193..2041
                     /locus_tag="PK812_03530"
     CDS             1193..2041
                     /locus_tag="PK812_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009465062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRJ68656.1"
                     /translation="MPLEIKVLDYGDIELESSFLVLGRDCGRVRRVPTYGFLVLGGPW
                     PIVVDTGYRHNQIMESLGMRGLQFHENMIENQLAKYGVRMGDVRYVLHTHLHIDHAGK
                     DELFPMNTTVIINRRELEYSVSGLMHPQYPAPDIKHLIDRLHTRDALRLEDLELTGMI
                     ELFPGCYMEAAGAHTEGSMNVHVDTVEGRATICGDVIYDFNDQIVNPFHELHANEPRV
                     TGNHGVSKREEKAAIKKLLNGSKFLLPVHDKPAKIAAGQVVGRMDMAVPGPVVQTLPA
                     RNWFAM"
     gene            2093..3679
                     /locus_tag="PK812_03535"
     CDS             2093..3679
                     /locus_tag="PK812_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HRJ68657.1"
                     /translation="MTTHVAVRPVFADLIDLWAPVRQIGTGFQFTEGPIWHPGEHFLL
                     FSDMPGDVRRRYDKGGIREVMKPANKCNGMTYDADLNLIVCEHATSTLVRERPDGRRE
                     VLAAHFEGMELNSPNDVCVSTDGAIYFSDPWYGRMPVYGIERPRQLGFQGVYRLPPGG
                     GKPQLLVDRYLFEQPNGLCFSPDERLLYINDTVQQLIRVFDVQADGTLARGRLFASGI
                     VSPSEPGVPDGMKCDALGNVWVCAPGGVWVYAPDGTLIGKVRIPELVGNLTWGGPDFR
                     TLFVTATHSVYAVETKVGPRVEPYMKHTAARGGTATAAGASIARNGTKKADPGFALDP
                     ARCALIIQDMQNDVVMEGGAFASSGSPAHCREQNAIANAVRLADAARRKGIPVIHVWF
                     IVEPGCPGVTMNAPLFEGLADAKALVRGSWGAAAVPGLEAQPGDHVVEKQRMSAWEGT
                     RLETVLKALRRDIVIVTGAWTNMSIEHTSRTGADKGYYMIVPEDCCSTMNAEWHTASV
                     NYALQNVAVVTDSSALIGAW"
     gene            complement(3751..4533)
                     /locus_tag="PK812_03540"
     CDS             complement(3751..4533)
                     /locus_tag="PK812_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRJ68658.1"
                     /translation="MSGPSRGDFLAIAGSKLEWRLFGAGDPPEIVLLHEGLGCVGLWG
                     DFPERLAARTGRSVLAYSRAGYGASSPVPLPRAMSYMHDEARHVLPALLDAIGFVRGV
                     LVGHSDGASIATIHAGDVVDRRVVGMTLIAPHFFVEDISVAAIAEARKAYETSDLREK
                     LARWHANVDNAFRGWNEAWLDPAFRHWDISACLETINCPVQVIQGTDDQYGTVRQVEV
                     ARARCSAPVETHLLPGVKHAPHREASETTLTLISQFIARHLG"
     gene            complement(4536..6104)
                     /locus_tag="PK812_03545"
     CDS             complement(4536..6104)
                     /locus_tag="PK812_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HRJ68659.1"
                     /translation="MSKTQQWMQPAGDYNAAVDMVDRNVAAGRGGKAAFIDPNETLTY
                     GELAERCNRFANGLRALGISPERRVVLIMLDTVDMPVAFWGAIKAGVIPIPVNTLLTP
                     EQWKFMIDDSRAEAVVVSPELYERAKPRLDEVGARRDLKVLVSGAGVPGVASMRALMA
                     EASPVAEAAPTHADEVAFWLYSSGSTGAPKGTRHFHASPAHTAHWFGQGVLGIREDDV
                     IYSAAKIFFAYGLGNAMSFPMSVGATTILWPERPTPDAVAAIMAKHNPTIFCGVPTLY
                     AAILANPALGKGAGSSRLRLCTSAGEALPEDIGSRWQQTTGVEIIDGIGSTEMLHIFV
                     SNRPGAVRYGTSGTPVPGYEARLLDDEGNVVPHGEIGELVIRGPSAAEGYWLQRGKSR
                     ATFRGEWTHTGDKYRVDADGYYHYCGRTDDMFKVSGIWVSPFEVESALITHPAVFEAA
                     VVGKEDEDGLTKPKAFVVLKPGIVGDDALFESLKAHVKDRAGMWKYPRWIDLRSELPK
                     TATGKIQRFKLRQE"
     gene            complement(6144..6854)
                     /locus_tag="PK812_03550"
     CDS             complement(6144..6854)
                     /locus_tag="PK812_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019998166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ68660.1"
                     /translation="MEPLLAVANVNTYYDESHILRDVSLSLNAGGTLGLMGRNGMGKT
                     TLLRTIMGLVRPRSGTVRFAGRDVTRAPPFRIARSGVAFVPEGRGVFASLTVAENLAI
                     AERPGPEGRQDWTVERVLDLFPRLKQRYANGGDQLSGGEQQMLAIARALLTNPRLLIL
                     DEATEGLAPLIRQEIWKTIRLVRESGIATLVVDKTISEVAAVADRVAILVKGQIVFEG
                     TPADLSADPDIMHRHLGV"
     gene            complement(6854..7609)
                     /locus_tag="PK812_03555"
     CDS             complement(6854..7609)
                     /locus_tag="PK812_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ68661.1"
                     /translation="MADAALALETVDLSKSFGGLRAVQNVSLQLAQGQLHAVIGPNGA
                     GKSTFVNLLTGQLRPTKGTILLAGQDITGTPSHRMPHLGVARSFQRTNILKSLSVLEN
                     VRLAAQALHPLGLRVLRSPRHGAELVDVARRALERVGLTGVEQSAAGILSHGAQRQIE
                     IAMTLVGDPKVILLDEPLAGMGQEEGERMAELLKSLARERALLLIEHDMDFVFAVADR
                     MTVLVDGTVLATGAPAAVRADKAVQTAYLGEHV"
     gene            complement(7602..8606)
                     /locus_tag="PK812_03560"
     CDS             complement(7602..8606)
                     /locus_tag="PK812_03560"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRJ68662.1"
                     /translation="MSEPARMLPQGKAGWALLAAIAVVLALLPPYVPSYYVGLITLAM
                     IGAMLALSLHLLVGGVGLVSLGHGAFYGLAAYVVFLMSPEGSPKPIWLTLPAAMLVSG
                     TVAFVVGGLSLRTKGFFFLMVTLAFGQMIFFVFHDTKLGGGTDGAYLAKPIISAFGAT
                     LDPRSFPRSQRGFPAYYVALVQLILTYLALAWLLRSMFGRVLEGIRVNEHRMEAMGFS
                     VMRYKLAAFTLAGAFAGAAGHMWALHSGFVNPELVSWHKSAEALLMILIGGIGSLAGA
                     VLGAFAFTALGEIAQQVTERKLLAEGVVILVAVLLLRNGLMGIRLGRGKTKGPGGGHG
                     "
     gene            complement(8603..9508)
                     /locus_tag="PK812_03565"
     CDS             complement(8603..9508)
                     /locus_tag="PK812_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRJ68663.1"
                     /translation="MILAGLLSQLLNGLQYGLLLFLISSGLTLIFGVLGVINLAHGSM
                     FMIGAYIAFLVAKSLGSLWFALPVAILAGVALGALLERVLFRHFYNREHLDQVLLTFA
                     LILIFEEMRSIIVGNDFHSVPVPAALDFSVPITNGFSYAAYRFAVIAACLVLAGALFW
                     WIERTKTGAIIRAAAEKPEVVDILGIDARTVHMTVFAVGTALAVTAGALAAPLTSVYP
                     NMGDSMLIISFVVVVIGGLGSVSGAFWSALLIGMIDTLGKAYGQLFVGSAFGNTLAAL
                     AAMSVYILMVAVLLFRPRGLFGGRA"
     gene            complement(9689..10885)
                     /locus_tag="PK812_03570"
     CDS             complement(9689..10885)
                     /locus_tag="PK812_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRJ68664.1"
                     /translation="MTKLITGAGIDRRRLLTGAAALVGASTVPTGWAIAQTKALKVGL
                     MLPYSGTFAQLGENITHAVEMVIAEKGGKLGGREIQLVRLDDESKPELGPQNAERLVK
                     RDNVDVLIGTVHSGVQMGIHKVVAESGTLCIVPNAGNNAVTRELCAKNVFRTSFTNWQ
                     PAYGMGLALGKRGVKKAAFVTWDYAAGQESAAAFKEGLEKGGGQLAQLLTLKFPETNF
                     QPLLAQIPGLNVEAVGAFFAGGGAVQFVKEYAAAGLKVPLVGSGFLTEGVLGPQGAAA
                     EGIETALHYGDGLENAKNAAFTKAFQAKAGRAADVYAVQGYDAAQLLAIGLEVAKGNL
                     DDEATLYKALRGAKIDSPRGPITMSPAQNVVQNIYLRKAQGGQNKVVGIAAENLADPG
                     AGCKLS"
     gene            complement(10991..12196)
                     /gene="boxA"
                     /locus_tag="PK812_03575"
     CDS             complement(10991..12196)
                     /gene="boxA"
                     /locus_tag="PK812_03575"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxA"
                     /protein_id="HRJ68665.1"
                     /translation="MAAAALTGDQGLRKQHLIDPEICIRCNTCEATCPVGAVTHDDVN
                     YVVDAEKCNFCMDCISPCPTGSIDNWRIVRAAWSIEDQLGWNELPPQEEFTATADGEA
                     IDDDVERLLASAHAGAGGKAMAPASASKPAVNLFRRANPVVATVQGNFRITAAETESD
                     IRHIVLAFGDTVVPVLEGQSIGIVPPGKREDGKPHDIRLYSIASARDGEKRNANNVGL
                     TIKRVSGGVASNYMCDLKVGDKVEVTGPFGTTFLMPQDPKANIIMICTGTGSAPFRAF
                     TERRRRAMQNVPGRLVMFFGARTPQELPYFGPLQKVPHNLLEKHLVFSRVPGTEKEYV
                     QDRIRTEGREMAGMLGSDATHIFICGLKGMEAGVEEALADVCRKHGLDWSQLKPRMRA
                     EGRYHVETY"
     gene            complement(12264..13730)
                     /gene="boxB"
                     /locus_tag="PK812_03580"
     CDS             complement(12264..13730)
                     /gene="boxB"
                     /locus_tag="PK812_03580"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HRJ68666.1"
                     /translation="MSTTASYVEGAVDYSTKIPNNINLVDDRRVLKALEQWHPGYIDW
                     WMTMGPEGFQTSEVYLRTAVSIDPKGWAKFDYVKMPEYRWGILLAPQDEQRLVNFGAS
                     KGEKAWQEVPGEHRAALRRLIVIQGDTEPASVEQQRHLGKTAPSLYDMRNLFQVNVEE
                     GRHLWAMVYLLHKYFGKDGRDEAEGLLERRSGDVDKPRMLGAFNEATPDWLSFFMFTY
                     FTDRDGKMQLESLAQSGFDPLSRTCRFMLTEEAHHMFVGETGVGRTIERTCEAMKAAG
                     IEDPYDITRIRALGVIDLPTIQKKANMHYSLSLDLFGSEISTNAANFYNGGLKGRFME
                     TKIEDDHILTNATYPVLKLVNGEAKLVEEPALTAINMRLRDDYSKDCQGGVNRWNKII
                     EKYGIKFQITLPSVAFHRHIGEFKDVHAAPDGRVLGDAEWIKNRDKWLPSKEDGDFIT
                     MLMQPCWERGKYAPWIAPPKVGIDNKGGDFEYVKIHQA"
     gene            complement(13900..15546)
                     /gene="boxC"
                     /locus_tag="PK812_03585"
     CDS             complement(13900..15546)
                     /gene="boxC"
                     /locus_tag="PK812_03585"
                     /EC_number="4.1.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-epoxybenzoyl-CoA dihydrolase"
                     /protein_id="HRJ68667.1"
                     /translation="MITFERSPETYRHWKLKFEGDVAELIMDVDENAGLFEGYQLKLN
                     SYDLGVDIELADALQRLRFEHPEVKVVVLKSGKDKVFCAGANIRMLGGAAHSHKVNFC
                     KFTNETRNGMEDSSEFSGQKFICAVKGTAAGGGYELALACDKIVLADDGNSTVSLPEI
                     PLLAVLPGTGGLTRVTDKRKVRRDRADAFCTVEEGFKGKRAVEWRLVDQSVPNSKFEQ
                     AIADMAKTLAATSDRPSGVPGIKLTPLGRRLDGDTLTYSAVTVEIDRAGRKATLTLLG
                     PTDAPPADAAGIVAQGDQFYPLRLSRELDDAILHLRVNELEIGTFVFKSKGDPAAVMA
                     YDALLDANRSNWLVREILNTWKRVLKRIDMTSRSLVTLVEPGSCFAGTLAEIAFACDR
                     SYMFDGAMKGDNRHATLTLSPLNFGAYPMSNGITRLQTRFLGEPDSIDAAQAEIGKTL
                     DATDAASLGLVTEAFDEVDWDDEIRIFLEERASFSPDGLTGMEANLRFGGPETMETKI
                     FGRLTAWQNWIFQRPNAVGPDGALKRYGTGVKGDFNLERV"
     gene            15756..16637
                     /locus_tag="PK812_03590"
     CDS             15756..16637
                     /locus_tag="PK812_03590"
                     /inference="COORDINATES: protein motif:HMM:NF006015.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HRJ68668.1"
                     /translation="MNAAAHQSLQEAIFAHVGASARSARLRAGVSRKKLAEAAGVSER
                     YLNELEKGVANASVSVLARIAQALDIDMGVLIPSSRPSREFSAQLAQVLARVAPGEEA
                     GVAGAITRYLDERQRNLKGVALLGLRGAGKSTLGRLLAEKHGLSFVSITREVEKRAGM
                     GLADLFNLGGVDAYRALENDVALDLARRRDRIVLETAGGIVGNTEALNTILGAFKTVW
                     LRASPEEHLARVAHQGDTRPMLGNPRALEHLMALLAQREPEYARAEARLDTSGKTPEA
                     CVIELERIAAVVLQRAA"
     gene            complement(16657..17526)
                     /locus_tag="PK812_03595"
     CDS             complement(16657..17526)
                     /locus_tag="PK812_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010971611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRJ68669.1"
                     /translation="MKLDDLIAIDVHTHAEVSCRQEQDVVWQAYEEAANTYFKAGKRP
                     TIAETVAYYREQKIGLVMFTVDSEFQIGNRRIPNEEIADAARDNADMMIAFASIDPHK
                     GRLGVREARDLIESGAVKGFKFHPTCQGFFPNDRLAYGLYELIAHYKLPAIFHSGHSG
                     IGTGMRGGGGLRLKYSEPMHLDDVAVDFPDMTIIIAHPSWPWTDQALSIALHKPNVYI
                     DLSGWSPKYFPPQIVQYANGQLRSKMLFGSDFPLIKPDRWIAEFKGAGFKPEIHDLIL
                     KKNAIRALKLDAA"
     gene            complement(17583..19430)
                     /locus_tag="PK812_03600"
     CDS             complement(17583..19430)
                     /locus_tag="PK812_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="feruloyl-CoA synthase"
                     /protein_id="HRJ68670.1"
                     /translation="MAASMRDVRLGTLGVAMERRPDGVMIIRSTEPLRPWETRLTDYL
                     VRWAREAPDRVYLAERVPDGGWRTMTYAQVHDAVRRIAAALLKRPLSVERPVAILSGN
                     GIDHALVGLAAMTIGVPYAAISVAYSLISSDFGKLKAIMAQLTPGLLFTTHAGPYARA
                     IAAACPADAEVVATSGTLPDRAVTPLADLLAVRHDAAVDAAEAKVDGDTIAKFLFTSG
                     STGNPKGVINTQRMLCANQAMMRQSLAFVADEPPVLLDWLPWSHTFGGNHNFNMVLAT
                     GGSFYIDEGKPTPEGIAATVRNMRDVSPTLYFNVPKGFEALAVHFAREPDVLKSFVRR
                     LKLNFYAGAALAPHVAAELSRLIEEATGEQILMTTSLGSTETAPSALACTREMSLRPG
                     VVGVPLPGVELKLVPSNGKLEARLRGPNITPGYWRDPEQTAKAYDEEGFYKLGDALKF
                     AVDGDPSGGFVFDGRVTEDFKLATGTWVSVGPLRGRFIAAMAPLVRDLVVAGHDRDDI
                     AGLVIPDMDGVRQVAGAAGQGLSDADVLALPEIRAAFRSRLDALARTATGSSSRVDRI
                     LVMAEPPSIDANEVTDKGSINQRSVLARRAALVEELYAEPRSPRVIHAG"
     gene            complement(19465..20262)
                     /locus_tag="PK812_03605"
     CDS             complement(19465..20262)
                     /locus_tag="PK812_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HRJ68671.1"
                     /translation="MARIDLPPTLKFHQEGPVGVIALARPEKRNALDDETILGLQRLF
                     SELPDAVRSVVLHGEGTHFSAGLDLSEVADAMDLYKGVRHSQMWHRAFNEIQFGRVPV
                     FTVLHGAVVGGGLELAASTHVRIAEPSAYYALPEGQRGIFLGGGGSVRITRLIGVPAV
                     QDLMLTGRTLSAEEGHVRGVSQYLVKEGEGMARAMELAQRAATNAPVSNFAILNALPH
                     IAESNPGSGFFTESLMASIAEHTPEARQRLKDFLEKRAAKVLHTKDP"
     gene            20434..20916
                     /locus_tag="PK812_03610"
     CDS             20434..20916
                     /locus_tag="PK812_03610"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRJ68672.1"
                     /translation="MKHPPKQTIASSMPAINYGQLDARMGYAIRRAQIAIFQDFFHTF
                     SGEDIKPAQYSILTIIENNPGLTQSQVAQSLGIKKTNFVAMIDALERRGFVKRQTVPQ
                     DRRSYALFLTEAGRSLMPRLHDLAEQHERRIIALTGRETYERLAEPIRAIGRLAPASD
                     "
     gene            21040..>21243
                     /locus_tag="PK812_03615"
     CDS             21040..>21243
                     /locus_tag="PK812_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004112303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRJ68673.1"
                     /translation="MPVAPLRETPLEQIRLRLPDLPGRLQDAGRYIVDNEFDAATRSM
                     RDIASAAGLQPATFTRLAQALGHA"
BASE COUNT         3808 a         6937 c         6708 g         3780 t
ORIGIN      
        1 gatttgcggg ctacgcgcgc ggcgccctcg tcttcgtcct gtcggacgga ctcgaacgcg
       61 gcgatcccgc cgcgatgatc cgcggcgtcc gccggctcgc ggcgagagcc ttccgcatcg
      121 actggttctc acccttggcc ggcgacgccg agtatcgccc cgaaaccgaa gcactcagcg
      181 ccattcggcc gctgctggcg agcctcggcg acgccagttc gacccagcgg atctgcaagt
      241 cgatcctctc cattgggacg gcgcgcgcag catgacggag ctggtcgatt cccatttcca
      301 catctggaac caagccgacc tgccatggct cgtcgggccg atgcagccgc gcatcttcgg
      361 tccctacgag ggtatccgcn nnnnnnnnng agccgatccg gcgcgactac ctgatcggcg
      421 agtaccttgc ggattgccgg acggccggca tcacgcaggc cgtctatgtc caggcgaact
      481 gggcgccaga gcgcgcgatg gatgaggtcg actacgtatc gcgcgcttcg acggaatccg
      541 gttttccgat catgatcgtg gctcatgccg atatgctcgc ggacgatgcg cgccccgctc
      601 ttggccagct agcgcgcaac cggcgcgttc gcggcgtcag gatgcaattg cactggcacg
      661 aaaatccact ctaccgtttc gccaagtctc ccgaccttgc acgcgatccg cgcttgctgc
      721 gcaatgtcgc ggcgctggcc gaattcggct ggagcttcga tctgcaggtg ttcgcgcatc
      781 agatggcagg ggcggcggat cttgcccggc attgcccgga cgtgaccttc gtgctccagc
      841 atgccggcat gctggaggat accgcgccgg caggcgtcac ggcctggcgg gacggcatgc
      901 gccgcctggc ggactgccca aatgtcgtga gcaagctctc cgggcttggc accttcctca
      961 gggcgaatga cccggcgcat atcgccttcg tcgtggacga aacgttggcg cttttcggcg
     1021 ctgaacgctg cctgttcggt tcgaacttcc cgatcgaaaa gctctggacg gactacggag
     1081 cgcttatcgg cgcttaccgc gcggccgtgc ctgccgaagc gcacgaacac atcttcaccc
     1141 gaaccgcatg ccgggtctac cggctgagcg aaaaaacctg aggaggacga taatgccttt
     1201 ggaaatcaaa gttttggact atggcgacat cgaactggaa tcgagctttc tggtcctcgg
     1261 ccgcgattgt ggacgcgtgc gccgcgtgcc cacctacgga ttcctggtcc tcggcggtcc
     1321 ctggccgatc gtcgtcgaca ccggctatcg ccacaaccag atcatggaga gcctcggcat
     1381 gcggggcctc cagttccatg aaaacatgat cgagaaccag ctcgccaagt acggcgtgcg
     1441 aatgggcgat gtgcgctacg ttcttcatac gcatcttcat atcgatcatg ccggcaagga
     1501 cgaattgttc ccgatgaaca ccacggtcat catcaaccgg cgggaactgg aatactccgt
     1561 gtcggggctg atgcatccgc aatacccggc gcccgacatc aagcacctga tcgacaggtt
     1621 gcacacccgc gatgcgctgc ggcttgagga cctcgaactg acaggcatga tcgaactgtt
     1681 ccccggctgt tacatggagg cggccggcgc gcataccgaa ggttcgatga acgtccatgt
     1741 cgatacggtc gagggacgcg ccacgatctg cggcgacgtc atctacgact tcaacgatca
     1801 gatcgtgaat ccgttccatg aactgcacgc caatgagccg cgtgtgaccg gcaaccatgg
     1861 cgtctccaag cgcgaggaaa aggcggccat caagaagctc ctgaacggct ccaagtttct
     1921 gctgccggtg catgacaagc cagcgaagat cgcggcgggt caggtcgtcg gacgcatgga
     1981 catggcggtt cctggacctg tcgtgcagac gctgcccgcg cgcaactggt tcgccatgta
     2041 gctctgaagg gcgggaagtc cctgcccgtt cgcttgcctg cgggggtttc gcatgacaac
     2101 gcacgtcgcc gtccggcccg tcttcgccga cctgatcgat ctgtgggcgc ccgttcgcca
     2161 gatcgggacc ggcttccagt tcaccgaagg cccgatctgg catcccggag agcacttcct
     2221 gctcttctcc gacatgcccg gcgacgtgcg ccgccgttat gataagggcg gcatccgcga
     2281 ggtgatgaag cccgcgaaca agtgcaacgg catgacctac gatgccgatc tcaacctgat
     2341 cgtgtgcgaa cacgcgacct cgaccctcgt gcgtgagaga ccggacggac ggcgcgaggt
     2401 gcttgccgcg catttcgagg ggatggaact caacagcccg aatgacgttt gcgtcagcac
     2461 ggacggcgcg atctacttct ccgacccctg gtatgggcgc atgccggtct acgggatcga
     2521 gcggccgcgt caactgggct tccagggcgt ctatcgcctg ccccccggcg ggggcaagcc
     2581 gcaacttctg gttgaccgtt atcttttcga acagcccaac ggtctatgct tctcgccaga
     2641 tgaaaggctg ctctacatca acgatacggt gcaacagctg atccgtgtgt tcgacgtcca
     2701 ggcggatgga acgctggcgc gcggaaggct gtttgcgtcc ggcatcgtgt cgccctcaga
     2761 acccggcgtt ccggacggca tgaagtgcga cgcgctcggc aacgtctggg tctgcgcgcc
     2821 cggcggcgtc tgggtttatg cgccggacgg cacgctgatt ggcaaggtcc gcattccgga
     2881 gttggtcggc aatctcacct ggggcggtcc tgacttccgc acgctgtttg tgaccgccac
     2941 gcactcggtc tacgccgtcg agacgaaggt cgggccgcgc gtcgagccct acatgaagca
     3001 cactgccgcg cgcggcggca ccgcgacggc ggccggagcg agcattgccc gcaacggcac
     3061 gaagaaggct gatcccggct ttgcactcga tcctgcgcgc tgcgcgctga tcatccagga
     3121 catgcagaac gatgtggtga tggaaggggg cgcctttgcc tcgtccggtt ccccggccca
     3181 ttgccgcgag cagaacgcca tcgccaatgc cgtgcgcctt gccgatgccg cgcgccggaa
     3241 aggcattccg gtgatccatg tctggttcat cgtcgaaccg ggttgtcccg gcgtgacgat
     3301 gaacgcgccc ttgttcgagg gcctcgccga tgcgaaggcg ctggtgcgcg gcagctgggg
     3361 ggcggcggcg gttccagggc ttgaggcgca gccgggcgat catgtcgtcg agaagcagcg
     3421 gatgagcgcc tgggaaggca cgcggctcga aaccgtgctc aaggcgcttc ggcgcgacat
     3481 cgtcatcgtg acaggcgcgt ggaccaacat gtcgattgaa cacacctcgc gcaccggggc
     3541 ggacaaaggg tactacatga tcgttccgga ggactgctgc tccaccatga acgcggagtg
     3601 gcacaccgcg tccgtgaact acgcgctgca gaatgtcgcc gtggtgacgg actcatccgc
     3661 gctgattgga gcctggtaac ggagccggcg taccgcaagg ttctgcttcg gctcgagata
     3721 cgtgtgaggt gatccgcctg cgccaagcat ctatccgaga tggcgcgcta tgaattgcga
     3781 tatcagcgtc aacgtcgtct cgctggcttc gcgatgcggc gcatgtttca cgccaggcag
     3841 gagatgcgtt tcaaccggcg cggagcaccg cgcgcgggca acctcaacct gtcgcaccgt
     3901 gccgtactga tcgtccgtgc cctggatgac ctgaacgggg cagttgatcg tttcgaggca
     3961 ggccgaaata tcccagtggc ggaaggcagg atcgagccag gcttcgttcc agccgcggaa
     4021 agcgttgtcg acgttggcat gccagcgggc cagcttttcc cgcagatcgc tggtttcata
     4081 ggctttccgc gcttcggcaa tggcggcaac ggaaatgtcc tcgacgaaga aatgcggggc
     4141 gatcagcgtc atcccaacga cgcgccgatc aacgacatcg ccggcatgga tggtggcgat
     4201 ggaggctccg tcggaatggc cgacaaggac gccgcgcaca aagccgatgg cgtcgagcag
     4261 cgccggcagc acgtggcgcg cctcgtcatg catgtagctc atcgcgcgcg gcagcggcac
     4321 gggcgaggag gcgccatatc cggcgcggga gtaggcaaga acactgcgcc cggtgcgcgc
     4381 cgccagacgt tcggggaaat caccccagag accgacacag cccaggccct cgtgcagcag
     4441 cacgatttcc ggtgggtcgc ctgcgccaaa aagccgccat tcgagcttgc ttcctgcgat
     4501 cgccaggaaa tcgcccctgg aagggccgct catcgctatt cctgccgcag tttgaagcgc
     4561 tggatcttgc cggtcgcggt cttcggcagc tcgcttctca gatcgatcca gcgcgggtac
     4621 ttccacatgc cggctcggtc cttcacatgt gccttgaggc tctcgaacag cgcatcgtcg
     4681 cccacaatgc ccggcttgag cacgacgaag gccttcggct tggtgagccc gtcctcatcc
     4741 tccttgccga ccacggcagc ctcgaagacc gcgggatggg tgatgagcgc cgattcaacc
     4801 tcgaagggcg aaacccagat gcccgacacc ttgaacatat cgtcggttcg gccgcagtag
     4861 tggtagtatc catccgcatc gacccggtac ttgtcgccgg tatgggtcca ttctccgcgg
     4921 aaggtcgcgc gggacttgcc acgctggagc caatagccct cggcggcgga ggggccgcgg
     4981 atgacaagtt cgccgatctc gccgtgcggt acgacattgc cctcgtcatc gagcagccgc
     5041 gcctcgtagc cgggcactgg cgtgccggag gtgccgtagc gaacggcgcc cggccggttc
     5101 gagacaaaga tatgcagcat ttcggtcgag ccgatgccgt cgatgatttc gacgccggtc
     5161 gtctgctgcc agcggctgcc gatgtcctca ggcagcgcct cgccagccga agtgcacagt
     5221 ctcagtcgcg atgacccggc accctttccg agggccgggt tggccaggat tgcggcgtag
     5281 agcgtcggca cgccgcagaa gatggtcggg ttatgcttgg ccatgatggc ggccaccgcg
     5341 tcaggcgtcg ggcgctcggg ccaaaggatg gtcgtcgcgc ccaccgacat cgggaaggac
     5401 atggcattgc ccaggccata ggcgaagaag atcttggccg cggaatagat gacgtcatcc
     5461 tcgcggatgc ccagcacgcc ctgcccgaac caatgcgccg tatgcgccgg gctggcgtga
     5521 aagtggcgcg tgcctttcgg ggcgccggta gagcccgacg aatagagcca gaacgccacc
     5581 tcatcggcat gggtcggcgc tgcttccgcg acaggcgagg cttccgccat cagcgcgcgc
     5641 atggaggcta cgcccggaac gcccgccccc gatacgagaa ccttgaggtc gcgccgcgct
     5701 ccgacctcgt ccagtcgcgg cttcgcgcgc tcgtagagct ccggggagac gacgaccgcc
     5761 tcggcgcggc tgtcgtcgat catgaatttc cactgctcgg gagtcagcag cgtattgacc
     5821 ggaattggaa tgacgccggc cttgatcgct ccccagaagg ccacgggcat gtccaccgtg
     5881 tcgagcatga tcagcacaac ccgccgctcc gggctgatgc cgagcgcgcg caaaccgttg
     5941 gcgaagcgat tgcagcgctc cgccagctcg ccataggtca gcgtttcatt gggatcgatg
     6001 aaggccgcct tgccgcccct gcctgcggcg acattccggt cgaccatgtc gacggctgcg
     6061 ttgtagtcgc cggcaggctg catccactgc tgggttttgg acatggcggg atcccctcga
     6121 tccggtcgat tgtcagttct agtctatacg ccgagatggc ggtgcatgat gtccggatcg
     6181 gccgagagat cggcaggcgt tccctcgaaa acgatctgtc ccttcacaag gatcgcgacg
     6241 cggtcggcga cggccgcgac ctctgaaatc gtcttgtcta ccaccagcgt ggcgataccg
     6301 ctctcccgca caagccggat cgtcttccag atttcctggc ggatcagcgg ggcaagcccc
     6361 tccgtcgctt catcgagaat gagcagacgc gggttcgtca gcaaggcacg ggcgatggcc
     6421 agcatctgct gttcgccgcc ggagagctga tcgccgccat tcgcatagcg ctgcttcagg
     6481 cggggaaaca gatcgagcac acgctccacg gtccagtcct gccggccttc cgggcctgga
     6541 cgctcggcga tggcgagatt ttccgcgacc gtaagcgacg caaagacgcc ccgcccctcg
     6601 ggcacgaaag ccacgccgct tcgggcgatg cggaacgggg gcgcgcgggt cacgtcgcgg
     6661 ccggcaaagc gcaccgtgcc ggagcgtggc cgcaccaggc ccatgatggt gcgcaggagc
     6721 gtggtcttgc ccatgccgtt gcggcccatc aggcccagcg tcccaccggc gttcagcgac
     6781 aatgaaacgt cgcgcaggat gtggctctcg tcgtagtagg tgttgacgtt ggcaacggcg
     6841 agcagcggtt ccatcacaca tgctctccga gataggcggt ctgcacggcc ttgtcggccc
     6901 gaacagccgc cggtgctccg gtagccagca ccgtcccatc caccagcacg gtcatgcggt
     6961 cggccacggc gaacacgaag tccatgtcgt gttcgatgag aagtagagcg cgctcacgag
     7021 ccagcgactt cagaagttcg gccatgcgct cgccctcctc ctgccccatg ccggcgagag
     7081 gctcgtcgag caggatcacc ttcggatcgc ccaccagcgt catcgcgatc tcgatctgcc
     7141 gttgtgcgcc gtggctgaga atcccggcgg cactctgctc gacaccggtc aggccgacgc
     7201 gctccagcgc gcgccgggcg acgtccacca gttccgcgcc gtgccgtggc gagcgcagga
     7261 cacgtaggcc aagcgggtgc agcgcctgcg ctgccaatcg aacgttctcc agaacagaaa
     7321 gggatttcaa tatgttggtt ctttggaacg agcgcgcgac gccgagatgc ggcatgcgat
     7381 gggacggcgt tccggtgatg tcctgcccag ccagcaggat cgtgcctttc gtcggtcgca
     7441 actggccggt cagcaggttg acgaacgtgg acttgcccgc cccgttcggc ccgatcacgg
     7501 catggagttg tccctgcgcc agttgcagtg agacattctg aacggcgcgc aaaccgccaa
     7561 acgacttcga caggtcgacg gtttcgaggg cgagcgcggc atcagccatg gccgccccct
     7621 ggtcccttgg tcttgccgcg accaagacgg atgcccatca gcccgttgcg cagcagcagc
     7681 accgccacaa gaatcacgac gccttccgcc agcagcttgc gttccgtgac ctgctgggcg
     7741 atctcgccca gcgccgtgaa cgcaaaagcg ccgagcaccg ctccggccag tgaaccgatg
     7801 ccgccaatca ggatcatcag cagcgcttcg gcggacttgt gccagctcac gagttccggg
     7861 ttcacgaatc ccgaatggag cgcccacatg tgcccggccg cccctgcgaa ggcgccggcc
     7921 agcgtgaagg cggccagctt gtaacgcatc accgaaaagc ccatcgcctc catgcgatgc
     7981 tcgttcacgc ggatgccctc cagcacgcgg ccgaacatcg agcgcagcag ccaggccagg
     8041 gcgagatacg tgaggatcag ctggaccagc gccacgtagt acgccgggaa cccgcgctgg
     8101 ctacgcggga aactgcgcgg atcgagtgtc gcgccgaagg ccgagatgat cggcttggcg
     8161 agataggcgc catccgtgcc gccgccgagc ttggtgtcgt ggaacacgaa aaagatcatc
     8221 tgcccgaagg cgagcgtcac catcaggaaa aagaagccct tggtgcgcaa ggaaaggccg
     8281 ccgacgacaa aggcaaccgt accggatacg agcatcgcag ccggcaaggt cagccagatc
     8341 ggcttcggcg acccctcggg cgacatcagg aagacgacgt aggccgcaag gccgtagaac
     8401 gcgccgtgcc cgaggctgac gagcccgaca ccgccgacaa gcaggtgcag gctcagcgcc
     8461 agcatcgccc cgatcatcgc cagggtgatc aggccaacat agtaggaggg gacatacggc
     8521 ggcagcagcg cgagcacgac ggcgatcgcc gccagcagcg cccagccggc cttgccctgg
     8581 gggagcatgc gggccggttc gctcatgccc tgcccccgaa aagcccgcgc ggccggaaca
     8641 gcagcacagc gaccatgagg atgtagacgg acatcgcggc gagcgccgcc agcgtattgc
     8701 cgaaggcact gccgacaaac agctgcccgt aggcttttcc gagcgtatcg atcatgccaa
     8761 tcagcaacgc cgaccagaac gcgccggaaa ccgatccgag gccgccgatc acgaccacca
     8821 cgaaggagat gatcagcatg ctgtcgccca tgttcggata gaccgatgtg agcggcgcgg
     8881 cgagcgcacc ggccgttacg gccagcgccg tgccgacagc gaagacggtc atgtgcaccg
     8941 ttctggcatc gattccgagg atatcgacga cttccggctt ttcagccgcc gcgcggatga
     9001 tggcgcccgt tttcgtgcgt tcgatccacc agaacagggc gccggccagc accagacagg
     9061 ccgcgatgac cgcgaaacgg taagcggcgt agctgaagcc gttggtgatc ggtacggaaa
     9121 aatccagtgc tgccggcaca ggcacggagt gaaaatcgtt gccgacgatg atcgagcgca
     9181 tctcctcgaa gatcaggatc agcgcaaagg tcagcagcac ctgatcgagg tgctcgcggt
     9241 tgtagaaatg gcgaaacagc acgcgttcca gcaaagcacc gagcgcgacg ccggccagga
     9301 tggccaccgg cagggcgaac cagagcgagc ccagcgactt cgccacgagg aaggcaatgt
     9361 aggccccgat catgaacatc gagccatgcg cgagattgat cacgccgagc acgccgaaga
     9421 tcagcgtcag cccgctcgaa atgaggaaca gcagcagccc gtactgcagg ccgttcagaa
     9481 gctgggaaag aaggccggcc aggatcatgg aaccgcagtc tgcgccgcaa ggtgcgcgcg
     9541 aaagaagcac gaggtcgccc caccgcaccg tctccctcgc acgggatcag gtcgcggaag
     9601 gccaggtgag actttgcccc tcacccgcgc ctgccgcttg cgttcggtgc gtcctcgccc
     9661 gtgaacggga gggggacgtc ttctgctgtc agctcagctt gcagcccgcc cccggatcgg
     9721 ccaggttttc agcggcaatg ccgacgacct tgttctgccc gccctgagcc ttgcgcaggt
     9781 agatgttctg cacgacgttc tgcgcaggcg acatcgttat cggaccgcgc ggactgtcga
     9841 tcttggcgcc gcgcagcgcc ttgtagaggg tcgcctcatc gtcgaggttg ccctttgcaa
     9901 cttcaagacc gatcgcgagc aactgggccg cgtcgtagcc ctgtacggcg tagacgtccg
     9961 ccgcccggcc ggccttggcc tggaaggcct tggtgaacgc cgcgttcttt gcgttctcga
    10021 ggccatcgcc gtagtgcaac gccgtctcga tgccttccgc agccgcgccc tgcggcccca
    10081 ggacgccttc ggtgaggaat ccggaaccga cgagcggaac cttgaggccg gcggccgcgt
    10141 attccttcac gaactgcacc gcgccaccgc ccgcgaagaa ggcgccaacc gcctcgacat
    10201 tcaggcccgg gatctgtgcc agcagaggct ggaagttcgt ctccgggaat ttcagggtca
    10261 gaagctgcgc cagctgtccg ccgccctttt ccaggccttc cttgaaggcg gcggccgatt
    10321 cctggccggc agcgtagtcc caggtcacga aggccgcctt cttgacgccg cgtttgccca
    10381 gcgcgaggcc catgccatag gccggctgcc agttggtgaa ggacgtacgg aagacattct
    10441 tggcgcacag ctcacgcgtc accgcgttgt tcccggcgtt cggaacgatg cagagcgtgc
    10501 cgctctcggc gacgaccttg tggatgccca tctgcacgcc ggagtgaacg gtgccaatca
    10561 gcacgtcgac attgtcgcgc ttgacgaggc gctcggcgtt ctgcggtccg agttccggtt
    10621 tggattcatc gtccagcctg accagctgaa tctcgcgtcc gcccagtttg ccgccttttt
    10681 ccgcgatgac catctccacc gcatgggtga tattctcacc cagttgggcg aacgtgccgg
    10741 aatacggcag catgaggccc actttcaggg cctttgtctg ggcgatcgcc cagccggttg
    10801 gtacggtcga tgctccgaca agcgccgccg ctccggtcag aagtcgccgc cggtcgatgc
    10861 cggcgcccgt gatcagcttc gtcatgggta tcctccctcc ttgtttcctc gcggctcacc
    10921 cgctcgtgga atttgttttg aatttcaact ctttaagact gaagacatgg gcgcctcaca
    10981 ctgctcgcct tcagtaggtc tcgacatggt agcgcccttc agcgcgcatt cgtggcttga
    11041 gctggctcca gtcaagcccg tgcttgcgac acacatcggc aagcgcttcc tcgacgccgg
    11101 cctccatgcc tttcaggccg cagatgaaga tatgggtcgc atccgatccg agcatcccgg
    11161 ccatctccct gccctccgtg cgaatgcgat cttgcacata ttccttttcc gtgccgggca
    11221 cgcgggagaa gacgagatgc ttttccagaa ggttgtgtgg cactttctgg agcggcccga
    11281 aatagggcag ttcctgaggc gtgcgcgccc caaagaacat cacgagacgt cccggcacat
    11341 tctgcatcgc ccgcctgcgc cgttcggtga aggcccgaaa cggcgcggaa cccgtgccgg
    11401 tgcagatcat gatgatattc gccttcgggt cctgcggcat caggaaagtc gtgccgaagg
    11461 gaccggtcac ctcgaccttg tcgccgacct tgaggtcgca catgtagttc gaggcgacac
    11521 ctccggacac acgcttgatg gtcaggccga cgttgttggc gttgcgcttc tcgccgtcgc
    11581 gcgccgaggc gatggagtag aggcggatgt cgtgcggctt gccatcctcg cgcttgccgg
    11641 gcggaacgat gccgatcgac tgcccttcca gcacgggcac caccgtatcg ccgaaagcca
    11701 gcacgatgtg ccggatgtcg ctctcggttt cggcggcggt gatgcggaaa ttgccctgaa
    11761 ccgtcgccac gacgggattg gcgcgccgaa acaggttcac ggcaggcttg gaggcggagg
    11821 ccggcgccat ggccttgcca ccggcgccag catgcgcgct ggccagcagg cgttcgacat
    11881 cgtcgtcgat cgcctcgccg tcagcggtcg cggtgaactc ctcctgtggc ggcagttcgt
    11941 tccagccgag ttgatcctcg atgctccagg ccgcgcgcac gatgcgccag ttgtcgatgg
    12001 agccggtcgg gcaagggctg atgcagtcca tgcagaaatt gcacttctcc gcatcgacca
    12061 cgtagttgac gtcgtcatgg gtcacagcac ccaccgggca ggtcgcctcg caggtgttgc
    12121 agcggatgca gatttcagga tcgatcaggt gctgcttcct gagcccctga tcgcctgtga
    12181 gcgcggcggc cgccatgcgt ttcccctcac gtcttggaag gtgcgcccct ggagcattct
    12241 ccttggggcg caccggccgg ccatcaggcc tgatggattt tcacatactc gaagtcgcca
    12301 cccttgttgt cgatgccgac tttcggcggc gcgatccacg gcgcatactt gccgcgctcc
    12361 cagcagggtt gcatcagcat cgtgatgaag tcgccatcct ccttcgaggg cagccacttg
    12421 tcacggttct tgatccattc cgcatcgccg aggacacggc catcgggagc cgcgtggaca
    12481 tccttgaatt cgccgatatg gcggtggaac gcgaccgatg gcagggtgat ctggaacttg
    12541 atgccgtatt tctcgatgat cttgttccag cggttcacgc cgccctggca atcctttgaa
    12601 tagtcgtcac gcaggcgcat gttgatcgcc gtcagcgccg gttcttccac cagcttcgcc
    12661 tcgccattga ccagcttgag caccgggtag gtggcgttgg tgaggatgtg atcgtcctcg
    12721 atcttcgtct ccatgaaacg gcccttcagg ccgccattgt agaagttcgc ggcgttcgtg
    12781 gaaatctccg atccgaacag gtccagcgac agcgaataat gcatgttcgc tttcttctgg
    12841 atggtcggca ggtcgatgac gcccagcgcg cggatgcggg tgatgtcgta ggggtcctcg
    12901 atgccggcgg ccttcatcgc ctcgcaggtc cgctcgatgg tgcggcccac gcccgtctcg
    12961 ccgacgaaca tgtggtgcgc ttcctcggtc agcatgaaac ggcaggtgcg cgacaaggga
    13021 tcgaagccgg actgggccag cgattccagc tgcatcttgc cgtcgcggtc ggtgaagtag
    13081 gtgaacatga agaacgacag ccagtctggt gtcgcctcgt tgaaggcgcc gagcatgcgc
    13141 ggcttgtcca catcgcccga gcgacgctcc agcagtccct ccgcctcgtc ccgtccgtcc
    13201 tttccgaaat acttgtgcag cagatagacc atcgcccaga ggtggcgccc ctcctccacg
    13261 ttgacctgaa acaggttgcg catgtcgtag agcgagggcg cggtcttgcc gaggtgacgc
    13321 tgctgctcga ccgatgccgg ttccgtgtcg ccctggatga cgatcaggcg acgcagcgcg
    13381 gcgcgatgct cgcccggcac ctcctgccag gccttttcac ccttggaggc gccgaaattc
    13441 accaggcgct gctcatcctg cggggcgagc aggatacccc agcgatattc cggcattttc
    13501 acgtagtcga acttggccca gcccttcggg tcgatcgaga ccgccgtgcg cagatagacc
    13561 tccgacgtct ggaagccttc cggccccatc gtcatccacc agtcgatgta gccgggatgc
    13621 cactgctcaa gcgccttcag cacgcgccga tcatccacaa gattgatatt gttcgggatt
    13681 tttgtcgaat aatcgaccgc tccctcgaca taggatgctg tagtgctcat cgtttcctca
    13741 catgtttagg caagtggcaa attggtgtca ttccggggcc gcgagcggag cccggaatcc
    13801 atcttcctgt gggacgtcaa acgattggat ggattccggt tgcgctcctg cggaacgccc
    13861 cggaatgaca cccggttcgc gaccaaccgt ctgcgcacct cacacgcgct ccagattgaa
    13921 atcgcctttg acgccggtgc cgtagcgctt cagcgcgccg tccgggccga ccgcgttggg
    13981 ccgctggaag atccagttct gccaggccgt cagccgaccg aagatcttcg tttccatggt
    14041 ctccggaccg ccgaagcgca ggttcgcctc catgccggtc aggccgtccg gcgaaaacga
    14101 ggcacgctct tccaggaaaa tgcggatctc gtcgtcccag tccacctcat cgaaggcttc
    14161 cgtgacgagg ccgaggctgg ccgcgtcggt ggcatccagg gtctttccga tctcggcctg
    14221 cgccgcatcg atgctgtccg gctcaccgag aaagcgcgtc tgcaggcgcg tgatgccgtt
    14281 cgacatcgga taggcgccga aattcagcgg cgacagcgtc agcgtcgcat ggcggttgtc
    14341 gcccttcatc gcgccgtcga acatgtagga gcggtcgcag gcgaaggcga tctccgccag
    14401 cgtgcccgcg aaacaggacc ccggctcgac cagcgtcacc agcgaacgcg acgtcatgtc
    14461 gatgcgcttg agcaccctct tccaggtgtt gagaatctcg cggaccaacc agttcgagcg
    14521 gttggcatcc agcagcgcgt cataggccat cacggcagcc ggatcgccct ttgacttgaa
    14581 gacgaaggta ccgatctcca gttcgttgac gcgaagatgc aggatggcat cgtccagttc
    14641 gcgcgacagg cgcagcggat agaactggtc gccctgggcc acgatgccgg cggcgtcggc
    14701 ggggggcgca tcggtcggac cgagaagggt aagggtggcc ttgcgcccgg cgcgatcaat
    14761 ctcaacggtg accgccgaat aggtcagcgt gtcgccgtcg aggcggcgcc ccagcggggt
    14821 cagcttgatg cccggcacgc cagagggccg atcggatgtt gcggcgagag tcttcgccat
    14881 gtctgcgatc gcctgctcga acttcgaatt cggcacggac tgatcgacca gccgccactc
    14941 gaccgcccgt tttcccttga agccctcttc caccgtgcaa aaggcgtcgg cgcgatcgcg
    15001 ccgcactttc cgcttgtcgg tgacgcgggt cagcccgccg gtgcctggca gaaccgcaag
    15061 cagtggaatt tcgggcagcg acaccgtcga gttgccatca tcggcaagca cgatcttgtc
    15121 gcaggcgagc gccagttcgt agccgccgcc agccgccgtg cccttcaccg cgcagatgaa
    15181 cttctggccg gagaattcgg acgaatcctc catgccgttg cgtgtctcgt tcgtgaactt
    15241 gcagaagttc accttgtggg aatgcgccgc gccgccgagc atgcggatgt tcgcgccggc
    15301 gcagaacacc ttgtccttgc cggacttcag caccacgacc ttcacttccg gatgttcgaa
    15361 ccgcaggcgc tgaagcgcat ccgcaagctc gatgtcgacc ccgagatcat aggaattgag
    15421 cttgagctgg tagccctcga acaatccggc gttctcgtcg acgtccatga tcaactcggc
    15481 aacatcgcct tcgaatttca gcttccagtg gcgataggtc tcgggcgagc gctcgaacgt
    15541 gatcatgggc ttccctctcg tttcaaaccg gacgatgcat tttaattccg catcttggtc
    15601 cggatggcat cgtgacatcc tcgtagcctg atccatttaa gaagtaaagt gcctatcacc
    15661 gagattcttc tgacaccctt tacgcgattt tccgccaatt gcgccacttg acgcgcgact
    15721 taatgcacta taattcatcc atcaaaggag attccatgaa cgccgccgct caccagtcgc
    15781 tgcaggaagc catcttcgcc catgtcggcg cttcggcgcg ttcggcgcgc ttgcgagccg
    15841 gcgtctcgcg caagaagttg gcggaagctg cgggcgtgtc ggagcgctat ctcaacgaac
    15901 tggagaaggg cgtcgccaac gcctccgtgt cggtgctggc gcggatcgcc caggccctgg
    15961 acatcgacat gggcgttctg attccatcca gccggccgtc ccgcgagttt agtgcgcagc
    16021 tcgctcaggt gctcgcgcgc gtcgcgccgg gagaggaagc cggcgtcgcc ggtgccatca
    16081 cccgctatct ggatgaacga cagcgcaacc tgaaaggcgt cgccctgctt ggattgcgcg
    16141 gtgccggcaa gtcgaccctt ggccggctgc tggccgaaaa gcatgggctg tcctttgtct
    16201 cgatcacccg cgaagtggaa aagcgtgccg ggatgggcct tgccgatctc ttcaatctcg
    16261 gcggggtcga tgcctatcgc gctctggaga acgacgtggc gctggacctg gcccgccgca
    16321 gagatcgcat cgtgctggag accgccggcg gcatcgtcgg caacaccgag gcgctgaaca
    16381 cgatcctcgg cgcgttcaag acggtctggc tccgggccag tcccgaagag cacctcgccc
    16441 gtgtcgccca tcagggggat acacgcccga tgctgggcaa tccgcgtgca ctggagcacc
    16501 tgatggcgct gctcgcgcaa cgcgagccgg aatacgcgcg cgccgaggcc cggctcgaca
    16561 cctcaggcaa gacgccggaa gcctgcgtca tcgaactcga acgcatcgcc gccgtggtgc
    16621 tgcagcgcgc ggcctgatca gcaccgccgg tgcggcctag gccgcatcca gcttcagcgc
    16681 gcggatcgcg ttcttcttca ggatcagatc gtggatttcc ggcttgaacc cggcgccctt
    16741 gaattctgcg atccagcgat ccggcttgat cagcggaaaa tccgagccga acagcatttt
    16801 cgagcgcaac tgcccgttgg cgtattgcac gatctgcggc ggaaagtact tcgggctcca
    16861 gcctgacagg tcgatgtaga cgttcggctt gtgcagcgcg atggagagtg cctgatcggt
    16921 ccacggccag gaggggtgcg cgatgatgat ggtcatgtcg gggaaatcga ccgcgacgtc
    16981 atcgagatgc atcggctcgg agtatttcag cctcagcccg ccgccgccgc gcatgccggt
    17041 tccaatcccg gaatgtccgg aatggaagat cgccggcagc ttgtagtggg cgatcaactc
    17101 gtagagccca taggcgaggc ggtcattcgg gaagaacccc tgacaggtcg ggtggaactt
    17161 gaatcccttc accgcgccgc tttcgatcag gtcgcgcgcc tcgcgcacgc ccagacgccc
    17221 cttgtgcggg tcgatggagg cgaaggctat catcatgtcg gcgttgtcgc gcgccgcatc
    17281 ggctatctct tcgttcggga tgcgccggtt accgatctgg aactcggaat cgaccgtgaa
    17341 catcaccagg ccgatcttct gctcgcggta atacgcaacc gtctcggcga tggtggggcg
    17401 cttgccggcc ttgaagtagg tgttcgccgc ctcctcgtag gcctgccaga ccacatcctg
    17461 ctcctgccgg caggaaacct cggcgtgggt atggacgtca attgcgatca ggtcatcgag
    17521 tttcatgctt ggttccggtc cgggtggaat gctgggcggg acaacagggg cgctcttgga
    17581 cgtcagcccg catggatgac gcgcggcgaa cggggttcgg catagagctc ctccacgagc
    17641 gccgcgcggc gcgccagtac cgagcgctgg ttgatcgagc ccttgtcggt cacctcattg
    17701 gcgtcgatcg acggcggctc cgccatgacg aggatgcggt cgacccggct cgacgagccg
    17761 gttgcggtcc tggccagcgc atcgagtcga ctccggaacg ccgcgcggat ctccggcagg
    17821 gccagcacgt cggcatcaga cagcccctgc cccgccgcgc ccgcgacctg ccgaacgcca
    17881 tccatgtccg ggatgaccag gccggcgatg tcgtcgcggt catgcccggc aacgacaagg
    17941 tcacgcacga gcggcgccat ggccgcgatg aaccgcccgc gcaggggccc gacgctgacc
    18001 caggttccgg tcgccagctt gaagtcctcg gtcacgcggc catcgaagac gaagccgccg
    18061 gagggatcgc cgtccaccgc gaacttcagc gcgtcgccga gtttgtagaa cccttcctcg
    18121 tcataggcct tggccgtctg ctcgggatcg cgccagtaac ccggcgtgat gttggggccg
    18181 cgcagacgcg cttccagctt gccgttggac gggaccagct ttagttcgac gccgggcagc
    18241 ggcaccccga ccacgcccgg ccgcagcgac atctcccgcg tgcaggcaag cgccgatggc
    18301 gcggtctcgg tcgagcccag gctcgtcgtc atcaggatct gctcgccggt cgcttcctcg
    18361 atcaggcgag agagttccgc ggcgacatgg ggcgccagcg ccgcgccggc atagaaattg
    18421 agcttcagcc gccgcacgaa ggatttcagc acgtcgggtt cgcgcgcgaa atgcacggcc
    18481 agcgcctcga agcccttcgg cacgttgaaa tagagcgtcg gcgagacatc gcgcatgttg
    18541 cgcaccgtgg cggcgatgcc ttcaggcgtc ggctttccct cgtcgatgta gaacgagcca
    18601 ccggtggcca gcaccatgtt gaagttgtgg ttgccgccga acgtatggct ccagggcagc
    18661 cagtccagca ggaccggggg ttcatcggcc acgaaggcca gcgactggcg catcatcgcc
    18721 tgattggcac acagcatgcg ttgcgtattg atcacgccct tcggattgcc ggtcgatccg
    18781 gacgtgaaca gaaacttggc gatggtgtcg ccgtcgacct tggcctccgc cgcatccacg
    18841 gcagcgtcat gcctcaccgc caggagatcg gccagcggcg tcacggccct gtcaggaagg
    18901 gttccggatg tcgcgacaac ttccgcatcc gccggacagg ccgcggcgat ggctcgggca
    18961 tagggcccgg catgggtggt gaagagcagg ccaggcgtca gttgcgccat gatcgcctta
    19021 agcttgccga aatccgagga aatcagcgaa taggccacgg aaatcgccgc atagggcacg
    19081 ccgatggtca tggccgcaag gccgacaagc gcatggtcga tgccattgcc ggacaggatg
    19141 gcaacaggcc gctccacgga cagcgggcgc ttcagcaggg cagcggcgat gcggcgcacg
    19201 gcatcatgca cttgcgcata ggtcatcgtc cgccagccgc catcgggcac gcgctcggcg
    19261 agatagaccc ggtccggcgc ctcgcgcgcc cagcggacga gatagtcggt gagacgggtt
    19321 tcccaggggc gcagcggctc ggtcgagcga atgatcatga cgccgtcggg gcggcgctcc
    19381 atcgcgaccc cgagcgttcc cagtcgcaca tcgcgcatgc tggcggccat cgtctcccct
    19441 ccccgtctca ggtcccgcgc gcggtcacgg gtctttggtg tgcagcacct tcgccgcccg
    19501 cttttcgaga aaatccttaa gccgctggcg tgcctccggc gtgtgctcgg cgatcgaagc
    19561 catgagcgat tccgtgaaga agccggaccc gggattgctc tcggcgatat gcggcaaggc
    19621 gttgagaatg gcgaagttgc tgaccggagc gttcgtggcc gcgcgctggg ccagttccat
    19681 ggccctggcc atgccctcgc cttccttgac gaggtactgg ctcacaccgc gcacatgccc
    19741 ttcttccgcc gaaagcgtgc ggccggtcag catcagatcc tgcaccgccg gcacaccgat
    19801 gagccgggtg atccgcaccg atccgccccc gccgaggaag atgccgcgct gaccttccgg
    19861 cagggcgtag taggctgagg gctcggcgat gcgcacatgc gtcgaagcgg cgagttcgag
    19921 cccgccgcca acgaccgcgc catgcagcac ggtgaacacc gggacgcggc cgaactggat
    19981 ttcattgaag gcgcgatgcc acatctgcga atggcgcacg cccttgtaga ggtccatggc
    20041 gtccgcgact tcgctgagat cgagccctgc ggagaagtgc gttccctctc cgtgcaggac
    20101 gaccgagcgg accgcatccg gcagttccga aaacaggcgc tgcaagccaa ggatcgtctc
    20161 gtcgtcgagc gcgttgcgct tttccggtcg cgccagcgcg atcacgccca ccgggccttc
    20221 ctgatggaac ttcagtgtcg gcggcaggtc gatccgcgcc atcgcatcct cccccggtca
    20281 catgcatgcg accagtgttc ttgctggcgc cggccgcgca atgaaggcgc aaccgccgca
    20341 gagtttagat agttatcttg cataactatc caataccgtc aatatcgccg ggccggccct
    20401 ggaatcccgg ggtcctgcgg aaagagccgt accgtgaagc atccgccgaa acagaccatc
    20461 gcgtccagca tgccggcgat caactacggc cagttggacg cccgcatggg ctatgccatc
    20521 cggcgcgcgc agatcgcgat tttccaggat ttcttccaca ccttttccgg tgaggacatc
    20581 aagccggctc aatactcgat tctcacaatc atcgagaaca accccggcct gacgcaaagc
    20641 caggtcgcgc agtcgctcgg catcaagaag acgaacttcg tggccatgat tgatgcgctt
    20701 gagcggcgcg gcttcgtcaa acggcagacc gtgccacagg atcggcgctc ctatgccctg
    20761 ttcctcactg aagccggccg cagcctgatg ccacggctgc acgatctcgc ggagcagcac
    20821 gaacgccgca tcatcgcgct gaccggccgc gaaacctacg agcgcctggc cgagccgatc
    20881 cgggccattg gccggctggc ccccgcgtcg gactagacag cgacgggacg acacccgcgc
    20941 ccaagcgttt cggcgcaaga gtcacaaact cgccaacctc ccaggccaga ccgagattca
    21001 gttgttgcag aacaagtgaa agcatgaaac acatgtttca tgcctgtagc accgttgcgc
    21061 gagacgccat tggaacagat ccgtctgcgg ctgccggacc tgcccggccg gctgcaggac
    21121 gccggccgct atatcgtcga caacgagttt gacgccgcca cccggtcgat gcgcgacatc
    21181 gccagcgccg caggattgca gcccgccacc ttcacgcggc tcgcgcaggc gctggggcat
    21241 gcg
//
LOCUS       DAOMXN010000022        19890 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_1602_length_19890_cov_10.746811, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000022 DAOMXN010000000
VERSION     DAOMXN010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 19890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19890
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1602_length_19890_cov_10.746811"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="PK812_03620"
     CDS             complement(<1..599)
                     /locus_tag="PK812_03620"
                     /inference="COORDINATES: protein motif:HMM:NF025878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68674.1"
                     /translation="MTQVDLDTATLTAQERLGAWSQAITAAFGPFAIANGDAERFHGR
                     VKVERRHDIRFIGLSYSGHGFVRRPSDVSRLDDAYYSLMRPIRGRLRLVQDGVTHLLE
                     PGHFYVVNHSLPYQTTPEDDYATEAIAFPPSALEARVARVQPFYALRDDPNCPRSALL
                     GTFLNHFAGARATWSDREFERLAAQLVDLVALAIVEPGTA"
     gene            complement(734..1252)
                     /gene="grpE"
                     /locus_tag="PK812_03625"
     CDS             complement(734..1252)
                     /gene="grpE"
                     /locus_tag="PK812_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HRJ68675.1"
                     /translation="MPLPEAPIDPVAALEADKADLKDKLLRTLADMENLRRRTEREVA
                     DSRLYAVTAFARDMLTVADNLRRALDSLSPEARAGMDAAIQAMVEGVELTERDFLKTL
                     ERHGVKKREPLGEKFNPNFDQAMFEVPDESKPSGTVVQVMQPGYVIGERVLRPATVGV
                     SKGGPKPEKPAQ"
     gene            complement(1375..4182)
                     /locus_tag="PK812_03630"
     CDS             complement(1375..4182)
                     /locus_tag="PK812_03630"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:NF003467.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HRJ68676.1"
                     /translation="MSSARPAGRGRIPAFLPPEGESLWPADAARLFEQALASIGGEPT
                     PDMVRAEIVRWGKTVLATARDQAKSILYAQGGTACAQALCAAQDSIIRSLFRYVTTRL
                     YPVANPTSGERIAVCAVGGYGRGTLAPGSDVDILFLLAHSTPWGESVAEAMLYPLWDL
                     GLKVGHATRTVEECLKASQGDLTVRTAVLEARLIDGDPALFAEFQKRFDKEVVAGSVA
                     EFVQAKVQEREARITRAGNSRYLVEPNVKEGKGGFRDLQTLFWIAQYVYRVKNRDDLV
                     AVGLFTPRELALFKRCEEFLWRVRCHMHFATGRAEERLNFELQRVLAEQLGYRTRGAL
                     SPVERFMKHYFLVAKEVGDLSAIVISALEAQESRPTALFSRFIGKFRRRRPVDGGKDF
                     IIDAGRITVADDDVFDRDPVNFIRLFWHADRHQIAIHPDAARLITLSLGRIDAALREN
                     RQANALFLDILVSRNDPEETLRRMHETGVLGRFIPDFARINAMMQFSMYHHYTVDEHT
                     LRAIGILASLDAGKLKEQHPVANEVLPTLPSRQVLYVALFLHDIGKGRAEPHEIVGER
                     IARELGPRLGLSSADTETAAWLVRHHLDMSQTAQTRDISDPRTIATFAETVQTIERLK
                     LLLVLTICDIKAVGPGVWNGWKGQLLRALYWETEILLAGGHTATERDARVQGARQKLR
                     EALPDWSDERFSAYAQRHNTGYWLKVDLDRQVRHAGLLARVDATGERFQTEISTSAFN
                     GVTEVTVLANDHPRLLSIVTGACAAAGANIAEAQIFTTTDGLALDSIFISRAFDSDED
                     ELRRAGRVAGHIIKALKGEIRLPDIVASRRKQAKASSAFVVTPNVTLDNGLSARFTVI
                     EITGLDRPGLLYELTTEIGKLNLNIASARIVTYGEKAVDVFYVTDLTSGKIEQPARQA
                     AIRRQLLDVLEPPALAPTGAT"
     gene            complement(4179..5141)
                     /gene="gshB"
                     /locus_tag="PK812_03635"
     CDS             complement(4179..5141)
                     /gene="gshB"
                     /locus_tag="PK812_03635"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HRJ68677.1"
                     /translation="MASRKLTVAVQMDPIDKIAIKGDSTFALMLEAEARGHSLLYYTT
                     DRLTMRDGRIFAAVRPLSVRDIEGDHFTLGDEERIDLSKTDVVLMRQDPPFDMQYITA
                     THLLERIHPETLVVNNPQHVRNAPEKIFVTEFPDLMPPTLITRDRREIEAFRAEHGDI
                     VMKPLYGNGGAAVFKVGARDPNFGSLYDLFSVTFREPWVCQRFLPQVTEGDKRIILVD
                     GEAAGAVNRVPAADDIRSNMVRGGAAKPTELSKREQEICARIGPALRERGLIFVGIDV
                     IDGNLTEINVTSPTGIRAIRRLGGPDLAVAIWDAIENKAGRSRS"
     gene            complement(5193..5558)
                     /locus_tag="PK812_03640"
     CDS             complement(5193..5558)
                     /locus_tag="PK812_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HRJ68678.1"
                     /translation="MPGGAKRSHATGFEAEWLAAAVLRLKGYSILAKRYTAAGGEIDL
                     VARRGGTLVFVEVKARQTMDEALGAISADKRRRVSRAARAYLARLPSLPATIRFDAIY
                     LAPGRLPRHHIAVAELDLD"
     gene            complement(5562..6455)
                     /gene="rsmI"
                     /locus_tag="PK812_03645"
     CDS             complement(5562..6455)
                     /gene="rsmI"
                     /locus_tag="PK812_03645"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HRJ68679.1"
                     /translation="MTASYTLFGLKAEALPLAPGLHLVATPIGNLKDITLRALEALAA
                     ADVILAEDKRVTKTLLAHYGITTPIMSYHEHNAEVVRPHVMARLHEGAALALVSDAGT
                     PLVSDPGFRLVESAIAQGIAVSALPGASAVLAGLVLAGLPTDRFFFEGFLPSRQTERR
                     RRIRALEAVPGTLVLFEAPHRLAESLSDLSEVLGDRPAAVARELTKKFETVRRGTLPE
                     LTRHYADVEKPRGEIVILVAPVDGAGETVTDDQIDARLIAEMETRSARDASALIAADL
                     GLPKRKVYARALALTDARRKE"
     gene            6704..7918
                     /locus_tag="PK812_03650"
     CDS             6704..7918
                     /locus_tag="PK812_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HRJ68680.1"
                     /translation="MSVTDRSPFKLVGPSRRHVAGGLAVSVAALVAGCADVNTTFPSV
                     ATPSGPTNQPTGNTIGNGPVRIALILPLSAPNGAVPAQALRNAAEMAIAEFNSQELTI
                     LVKDDKGTPEGAQAAAQEALSEGAEAILGPLFAPAVSAAASVARGASRPVIAFSTDAS
                     VAARGVYLLSFMPQSDVRRIVSFATQRGKKSFAALIPQSAYGSVAEAEFAQAVTSSGA
                     RLAMVERYQPNKASIDAAIAKIKAGAQQVDSLFIPDDGNGLPGVGAALQGAGISSRSM
                     QLLGTGVWNEARVFRTAALSGAWFAAPENAGFQNFAQRYRSRFNSDPTRIATLAYDAT
                     ALLSALVRTQGAQRFSEGVLTNASGFAGQDGVFRFRQDGTNDRGLAIYQLGNGSAQVI
                     QPAPRTFAAGSA"
     gene            complement(7926..9089)
                     /gene="hemW"
                     /locus_tag="PK812_03655"
     CDS             complement(7926..9089)
                     /gene="hemW"
                     /locus_tag="PK812_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HRJ68681.1"
                     /translation="MSALPPFDAGFGVYVHWPFCASKCPYCDFNSHVRRGGIDEERFL
                     AAFRREVAGWAAQAPGRVVSSIFFGGGTPSLMRPETVGGILDAIAQHWAIDSSVEVTL
                     EANPTSVEAGRFRGYRAAGVNRVSLGVQALIDTDLKFLGRLHSVAEALAAIAVASSVF
                     ERFSFDLIYARPGQTPQAWRSELVEALAFAPHHLSLYQLTIEPDTAFDALAQAGKLHV
                     PGAEEARILYDVTQDVCDRQGLPAYEISNHARPGEECRHNLLYWRYGEYAGIGPGAHG
                     RLWTVDGRLAMANEKAPEAWCSLVERDGGGCITTERLSQENEADEFLLMGLRLAEGID
                     PKRFERLRGRPLDERRIAVLEVEGLVARDTTGRLRVTPAGFPVLDAVVADLAA"
     gene            complement(9086..9694)
                     /gene="rdgB"
                     /locus_tag="PK812_03660"
     CDS             complement(9086..9694)
                     /gene="rdgB"
                     /locus_tag="PK812_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018045121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HRJ68682.1"
                     /translation="MTGRRLAGRVVLATHNAGKLAEMVDLLAPFGVEVVSSGALGLPE
                     PAETGTLFSENAAIKARAAAVATGLPAIADDSGLCVDLLDGAPGLFTADWAGSPRNFT
                     RAMERLRFELERRGHDASGARAHFVSAIVVAWPDGGEQVFEGRVHGTLAFPPRGPEGF
                     GYDPCFVPLGEIRTFGEMPMAEKKKHSHRARAIAAMTAACFR"
     gene            complement(9694..10407)
                     /gene="rph"
                     /locus_tag="PK812_03665"
     CDS             complement(9694..10407)
                     /gene="rph"
                     /locus_tag="PK812_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012319894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HRJ68683.1"
                     /translation="MRPSKRAPDELRKVTLERGVARYAEGSCLVKFGETHVLCTASLE
                     ERGPPWLRGSGKGWVTAEYAMLPRATLERTRREVTSGKPSGRTQEIQRLIGRALRAVV
                     DLSALGERQIVIDCDVLQADGGTRTAAITGGWVALHDCLAWMKAREMVKNLPLRDHVA
                     AVSCGIYNGTPVLDLDYPEDSSAQTDANFVMTGSGGIVEVQGTAEGAPFSQEELLKLL
                     ELAGKGIGRLVDLQKMAVA"
     gene            10534..11619
                     /gene="hrcA"
                     /locus_tag="PK812_03670"
     CDS             10534..11619
                     /gene="hrcA"
                     /locus_tag="PK812_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HRJ68684.1"
                     /translation="MTADLSKPSSGRPGDIDERSREIFRRIVDGYLATGEPVGSRQIS
                     RQLAVQLSPASVRNTMQDLEDLGLIYAPHTSAGRLPTEIGLRLFVDAMLEIGDVSTDE
                     KARIEAEVQASPGAKGRSFEDTLAEASRLLSGLSGGAGLVAATKGSKRWKHIEFVRLD
                     PAQALIVLVGEDGSLENRLLPLPPGLPASALFEASNYLNARLRGRALGEVRAEIEAQR
                     AEMERELDALTARLVDSGLASAAESIDGVPQLIVRGQANLLENVTAADDLERIRRLFS
                     DLEAKKDVIDLLSRAENADGVRIFIGSENKLFSLSGSAMIAAPFRNGQQRIVGVLGVI
                     GPTRLNYARIVPLVDYTARVVSRMLDR"
     gene            complement(11677..13287)
                     /locus_tag="PK812_03675"
     CDS             complement(11677..13287)
                     /locus_tag="PK812_03675"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF006822.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HRJ68685.1"
                     /translation="MVTSGKHNTTVGANTFGFDSPRTILCNFMPPALYEESIRRGEAQ
                     ILKGGAIVAETGIHTGRSPKDKFTVRDATTADTVWWDQNQAMTPEAFDTLLADFKAHA
                     KGKDLFLQDLIGGADPRNRVRVRVYTELAWHSLFIRNLLIRPDVADLKDFVPEMTIVD
                     LPSFRADPKRHGCKSETVIAIDLSRKLVLIGGTAYAGEMKKSVFTYLNFVLPTNNVMP
                     MHCSANVGKDGDVALFFGLSGTGKTTLSQDPNRVLIGDDEHGWGQDGVFNFEGGCYAK
                     TIRLSPEAEPEIYATTQRFGTVLENVVFDPDTRIADFDDETKTENTRCAYPLHFISNS
                     SKTGRAGHPRNIIMLTCDAFGVMPPIAKLTGAEAMYHFLSGYTAVVAGTERGVTEPQA
                     TFSTCFGHPFMPRHPSVYGNLLRDLIAKHGADCWLVNTGWTGGKAGVGRRMPIRVTRR
                     LLTAALDGSLNRANFRRDPYFGFSVPESVPGVEPHILQPVKTWQDKAAFNETAKKLIA
                     MFEKNFKTFESHVGRDVVAAAPQRLIAA"
     gene            complement(13544..14305)
                     /locus_tag="PK812_03680"
     CDS             complement(13544..14305)
                     /locus_tag="PK812_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008598592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRJ68686.1"
                     /translation="MAVDPEVAAKMAPGFDPLPQAKAILRTARSGTLATLQGDGAPFA
                     SLVSMATDMDGAPLILISRLASHTRQIEADGRCSLLLSQPGKGDPLAHPRLTVVATAG
                     RIDRDSAEGARVRRRFLARQPKAELYVDFPDFSFWRLAPRHVYLNGGFARAWDGPASD
                     VMTDLSGADALVDLEADALAHMNADHADAVRLYASRLCGGGAGPWRATGIDPEGMDLA
                     FGDQTLRLVFPQRVVDGATLRSVLADLAKQARADT"
     gene            14478..15176
                     /locus_tag="PK812_03685"
     CDS             14478..15176
                     /locus_tag="PK812_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRJ68687.1"
                     /translation="MPTIALVDDDRNILTSVSIALEAEGYRVQTYTDGASALDGLRQN
                     PPDMAIFDIKMPRMDGMELLRRLRQKSDLPVIFLTSKDEEIDELFGLKMGADDFIKKP
                     FSQRLLVERVKAVLRRAGAKDVAAPKEADARALERGQLHMDPERHACTWKGQPVTLTV
                     TEFLILQALAQRPGVVKSRNALMDAAYDDQVYVDDRTIDSHIKRLRKKFKVVDDDFDM
                     IETLYGVGYRFKEA"
     gene            15198..16988
                     /locus_tag="PK812_03690"
     CDS             15198..16988
                     /locus_tag="PK812_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stimulus-sensing domain-containing protein"
                     /protein_id="HRJ68688.1"
                     /translation="MLDRNPLPEASDRDVANPRSGTTGADSAQAAPGPVHRLSRALAL
                     RLSSSVARRIALLNLAGLLALLVGFLWLIQTRDSVIDARVQSLFTQAEIIASAVAASA
                     SVETDAITLDPDKLLQLQVGEGTSGAADDMRSAIEFSINPERVAPVLRRLVSPTRNRA
                     RIFDRDGIMLLDTRAFYSAGEIARLDLPPEPEEFTLFERSWSAIKRRFGRMDLPAYEE
                     TGNARAYQEVGRALLGAAGSVVRVSANGQTIVSVAVPVRRFRSVQGALMLTTQEGDID
                     RIIAEERFSILMVFIIAAGVMLVLSFLLAGTIAEPLRKLAEAADRVRRGTRARQEIPD
                     FSGRADEIGHLAGALRDMTRALYNRIEGTERFAADVAHELKNPLTSLRSAVETLPLAR
                     TDEARSRLLSVIQHDVRRLDRLITDISSASRLDAELARAEASPVDIALLLETVVAMAN
                     DVRRDDGVTVRLQVMPESRRIPGALVVNGHDSRLVQVFNNVIDNARSFSPPGGTVDVR
                     CDASADAVQITIDDTGPGIPPHALTRIFERFYTDRPNQGFGQNSGLGLSISQQIVEAH
                     HGTIRAENRLDGDGRTLGARFTVTLLREAA"
     gene            16985..17443
                     /locus_tag="PK812_03695"
     CDS             16985..17443
                     /locus_tag="PK812_03695"
                     /inference="COORDINATES: protein motif:HMM:NF019118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphatase C-terminal
                     domain-containing protein"
                     /protein_id="HRJ68689.1"
                     /translation="MSIAGITLHAGCVVIGEKAVLLTGKSGAGKSSLARALVDTAVAE
                     GRFAAMVADDRVLISRHGDRLVARPHPRIAGLQEIRGFGIVRGPFEPAAVIALVLELS
                     AGRQPRMPEATGETAHLLGMSVPSLRLYGNRPVAENAAITRIRLRTATVF"
     gene            17564..17965
                     /locus_tag="PK812_03700"
     CDS             17564..17965
                     /locus_tag="PK812_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003606016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRJ68690.1"
                     /translation="MIGMVLVSHGRLANEFKAAMEHVVGPQKQVETVCIGPEDDMEAR
                     RLEIVEAIHAVNSGSGVVVLTDMFGGTPSNLAISAMNGTGVEVVAGINLPMLVKLASV
                     RDACCLADAVVQAQEAGKKYINVASRVLAGK"
     gene            17962..18273
                     /locus_tag="PK812_03705"
     CDS             17962..18273
                     /locus_tag="PK812_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015821861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HRJ68691.1"
                     /translation="MNDEPCPDTIIPEGALARAMPIVNRKGLHARASAKFVQCAERFE
                     CDIQVHRCGETVGGTSIMGLLTLAAAKGTTITVTARGSDAEAALDALAELLANRFGED
                     E"
     gene            18386..19780
                     /gene="ahcY"
                     /locus_tag="PK812_03710"
     CDS             18386..19780
                     /gene="ahcY"
                     /locus_tag="PK812_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010685907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HRJ68692.1"
                     /translation="MESIVKDMSLAAFGRKEIELAESEMPGLMATRAEYGAAQPLKGA
                     RIAGSLHMTIQTAVLIETLKALGADIRWVSCNIYSTQDHAAAAIAAAGIPVFAYKGET
                     LTEYWDYTAKLFDWHGGGMPNMILDDGGDATMFVHLGLRAEQGDAAFLDKPGSEEEEI
                     FFALLKKKLAEKPKGWFAELAKSIRGVSEETTTGVNRLYQFAREGKLLFPAINVNDSV
                     TKSKFDNLYGCRESLVDAIRRGTDVMLSGKVAFVAGFGDVGKGSAASLRQAGCRVMVS
                     EVDPICALQAAMEGYEVVTTEDALPRADIFVTATGNKDIITVDHMRAMKDRAIVCNIG
                     HFDNEIQVAALRNFKWTNIKPQVDEITFPKGNRIILLSEGRLVNLGNAMGHPSFVMSA
                     SFTNQTIAQIELWTNPGKYEKKVYTLPKHLDEKVAMLHLAKVGAKLTKLRPDQASYIG
                     VPENGPFKPDHYRY"
BASE COUNT         3517 a         6541 c         6449 g         3383 t
ORIGIN      
        1 gccgttcccg gctcgacgat cgccagcgcc acgagatcga ccagctgcgc cgccaggcgc
       61 tcgaactcgc ggtcgctcca ggtcgcgcgg gctccggcga agtggttcag aaacgtgccg
      121 agcaacgcgc tgcgcggaca gttcggatcg tccctgagcg cgtagaacgg ctgcactcga
      181 gcaacccgcg cctcgagcgc ggacggcgga aaggcgatgg cttccgtcgc gtagtcgtcc
      241 tccggcgtgg tctggtaggg cagcgaatgg ttgacgacgt agaaatgccc cggttcgagc
      301 aggtgcgtga cgccgtcctg caccagtcgc aagcgtccgc gaatggggcg catcaggctg
      361 tagtaggcat catccagacg tgacacgtcg ctgggccggc ggacaaagcc atggccgctg
      421 taactcaggc cgatgaagcg gatgtcgtgg cgacgctcca ccttgacgcg cccgtggaac
      481 cgctcggcat cgccattggc gatggcgaac ggaccgaaag cggcggtgat tgcctgcgac
      541 caggcaccca gccgttcctg cgccgtcaaa gtcgcggtgt cgagatcgac ctgcgtcatg
      601 gcaccttaac ccatggcaac ggccgattgt ggaccggtgg cgcgcacctg gcaagcatcg
      661 gccaaaagcg tgctatcgcc gcgttccgac gcaggtcctg gtgtccgtgg ccccggtcca
      721 gccgatgcag gtgctactgc gccggctttt ccggcttggg accacctttc gaaaccccga
      781 ccgtcgcggg acgcaggaca cgctcgccga tgacataccc cggctgcatg acctgaacga
      841 ccgtgccgct tggcttgctc tcgtctggaa cctcgaacat cgcctggtcg aagttggggt
      901 taaacttctc gcccagcggt tcacgctttt tcacgccgtg acgctccagg gtcttgagaa
      961 agtcgcgttc cgtcaactcg acgccctcga ccattgcctg gatcgcggca tccatgccgg
     1021 cgcgcgcctc aggcgacagg ctgtcgagcg cccggcgcag gttgtccgcc acggtgagca
     1081 tgtcgcgcgc aaatgccgtg accgcgtaga gtcgcgagtc ggcgacttcc cgctcggtgc
     1141 gccggcgaag gttctccatg tccgccagcg tgcgcagcag cttgtctttc aggtccgcct
     1201 tgtcggcctc cagggccgcg accggatcga tcggcgcctc tggcaatggc accaaatctt
     1261 ccggcgtcgt ctctggctgc aacttgtcgc cgtccgtcac gtcgttacct cgaatgcaac
     1321 agggttgttg ggagtgcccc cgatatcgag gcaccgccac cgaaaatcaa gggttcaggt
     1381 cgcgccggtt ggcgccagcg ccggtggctc gagcacgtca agcagctggc ggcggatggc
     1441 ggcctgtcgc gccggttgct cgatcttccc gctggtcaga tcagtgacat agaaaacgtc
     1501 aaccgccttt tctccatagg tgacgatgcg cgcggacgcg atgttcaggt tgagcttgcc
     1561 gatctcggtg gtcaattcat agagcaggcc gggccggtcg agcccggtga tttcgatcac
     1621 cgtgaaccgt gccgacagac cattgtcgag cgtcacgttg ggcgtgacga caaaggcgga
     1681 gctggccttg gcctgcttgc ggcgggaagc cacgatatcg ggcagccgga tttcgccctt
     1741 cagggccttg atgatgtggc cggcgacgcg cccggcgcgg cgcagttcat cctcatcgct
     1801 gtcaaaggcg cgggaaatga agatggaatc gagcgcgagc ccgtcggtgg tggtgaagat
     1861 ctgcgcctcc gcgatattgg cgcccgccgc tgcacaggcg ccggtgacga tcgacagcag
     1921 ccggggatga tcattggcca ggacagtcac ttccgtcacg ccattgaacg cgctggtcga
     1981 gatttcggtc tggaaccgct cgcccgtcgc atcgacgcgg gcgagcaatc cggcatggcg
     2041 cacctggcga tcgagatcca ccttcagcca gtagccggta ttgtgccgct gcgcataggc
     2101 ggaaaagcgc tcgtcgctcc agtcgggaag ggcctcgcgc agcttctggc gcgccccctg
     2161 aacgcgcgcg tcacgctcgg tcgcggtgtg accgccggca agcagaattt ccgtctccca
     2221 gtagagggcg cgaagaagct gacctttcca accgttccaa acgccgggtc ccacagcctt
     2281 gatgtcgcaa atcgtgagca ccagcagcaa cttgagccgc tcgatggtct gcaccgtctc
     2341 ggcgaaggtg gcgatcgtgc gcggatcgga gatgtcccgg gtctgcgccg tctgcgacat
     2401 gtccaggtgg tgccggacca gccaggccgc ggtctccgta tcggcggagg acaagccgag
     2461 ccggggaccg agttcgcggg cgatgcgctc gccgacgatc tcgtgcggtt cggcgcggcc
     2521 cttgccgatg tcgtgcagga acagggcgac atacagcacc tggcgcgagg gcaaagtcgg
     2581 caggacttcg ttggcgacag gatgctgttc cttgagcttg ccggcatcaa gcgaagccag
     2641 gatgccgatg gcgcgcagcg tgtgctcgtc caccgtataa tgatggtaca tcgagaactg
     2701 catcatggcg ttgatgcgcg cgaaatccgg aatgaacctg ccgagtactc cggtctcgtg
     2761 catgcgccgc agggtttcct ccggatcgtt gcgcgagacc aaaatgtcca ggaacagggc
     2821 gttggcctgc ctgttctccc tgagagcggc gtcgatccgg ccgagcgaga gcgtgatcag
     2881 gcgggcagcg tcggggtgaa tggctatctg atgccgatcg gcgtgccaga acaggcggat
     2941 gaaattgacc ggatcacggt caaagacatc gtcgtcggcg accgtgatgc gcccggcgtc
     3001 gatgatgaag tccttgccgc catccacggg acgccgccgg cgaaacttgc cgatgaagcg
     3061 cgagaacagc gcggtcggcc gcgattcctg cgcttcgagc gcggaaatca cgatcgccga
     3121 cagatcgccg acctccttgg ccaccaggaa gtagtgcttc atgaagcgtt ctacgggtga
     3181 gagcgcgccg cgggtccgat agccgagttg ttcggccagg acccgttgca gttcgaagtt
     3241 gaggcgctcc tccgcccggc cggtggcgaa atgcatgtgg cagcgcacgc gccagagaaa
     3301 ctcctcgcag cgcttgaaca aggcaagctc gcgcggcgtg aacagaccga cggcgaccag
     3361 atcgtcccga ttcttcacac ggtagacgta ttgcgcaatc cagaacaggg tctgcaggtc
     3421 gcggaagccg cccttgcctt ccttgacatt gggctcgacg agatagcgcg agttgccggc
     3481 gcgggttatg cgcgcctccc gctcctgcac cttggcctgc acgaattcgg cgaccgagcc
     3541 agcgaccacc tctttgtcga accgcttctg gaattcagcg aacagcgccg ggtcgccatc
     3601 gatcaggcgc gcctccagca ctgctgtgcg caccgtgaga tcgccctgcg aggctttcag
     3661 gcattcctcg acggttcgcg tcgcatggcc gaccttcagg ccaaggtccc agagcggata
     3721 gagcatcgcc tccgcgacgc tctcgcccca gggcgtcgaa tgggcgagca ggaacaggat
     3781 gtcgacatcc gagcccggcg ccagggtgcc gcggccgtag ccgccgacgg cgcagaccgc
     3841 gatgcgttcg cccgaggtgg gattggcgac cggatagagc cgggtcgtga cgtatcgaaa
     3901 cagcgaccgg atgatcgaat cctgcgccgc gcacagggcc tgggcgcaag ccgttccgcc
     3961 ttgggcgtac aggatcgact tggcctgatc gcgtgccgtc gccagcacgg tcttgcccca
     4021 gcgcacgatc tccgcgcgga ccatgtccgg cgtcggctcc ccgccgatgg aggcaagcgc
     4081 ctgctcgaac agccgggcgg catcggccgg ccacagcgat tccccctccg gcggcagaaa
     4141 cgccggaatc cggccacgcc ccgccggtcg cgccgaactc acgacctcga ccttccggcc
     4201 ttgttttcaa tcgcatccca gatggcgacg gcaaggtcgg gtccgccaag acgccggatc
     4261 gcccggatgc cggtgggcga cgtcacattg atttcggtga gattgccatc gatgacgtcg
     4321 atgccgacga agatgagacc cctttcccgc agcgcaggac cgatgcgggc gcagatttcc
     4381 tgctcccgct tcgacagttc ggtcggtttc gccgcgccgc cccgcaccat gttcgagcgg
     4441 atgtcgtccg cggccggcac gcgattgacg gcgccggccg cctcgccatc gaccaggatg
     4501 atgcgcttgt cgccttcggt gacctgcggc aggaaccgct gacacaccca gggctcgcgg
     4561 aaggtgacgg agaacagatc atacagcgag ccgaaattgg gatcgcgcgc gccgaccttg
     4621 aagacggcgg ccccgccgtt gccatagagg ggcttcatga caatgtcgcc gtgctcggcg
     4681 cgaaaagcct cgatttccct gcggtcccgc gtgatcagcg tcggcggcat caggtccggg
     4741 aactcggtca cgaagatctt ctcgggcgca ttgcggacat gttgcggatt gttgacgacc
     4801 aaggtttccg ggtgaatgcg ctccagcaga tgcgtggcgg tgatgtactg catgtcgaac
     4861 ggtggatcct gccgcatcag caccacatcc gtcttcgaca ggtcgatgcg ctcctcgtcc
     4921 cccaaggtga aatggtcgcc ctcgatgtcg cgcaccgaca ggggccggac ggcggcgaag
     4981 atgcgcccgt cgcgcatcgt gagcctgtct gtggtgtagt agagcaggct gtgtccgcgc
     5041 gcctcggctt cgagcatgag cgcgaaggtg gaatcgccct tgatggcgat cttgtcgatc
     5101 gggtccatct gaacggcgac ggtgagcttt cgggaggcca tggcgaacct tgggaacagc
     5161 aggaggatac agcaagccag ttgcgtgcga ggtcagtcca agtcaagttc ggcaacggcg
     5221 atgtgatggc gtggcaggcg tccgggcgcg agatagatcg catcgaagcg gattgtcgcg
     5281 ggcaaggacg gcagtcgcgc gaggtaggcc ctggcggcgc gggagacgcg ccgccgcttg
     5341 tccgccgaga tggcccccag cgcctcgtcc atcgtctgcc gggccttgac ctcgacgaag
     5401 accagggtgc cgccacgtcg cgcgacaagg tcgatctcgc cgcccgccgc agtataccgc
     5461 ttcgccagga tgctgtagcc tttgaggcgc agcaccgctg cggccaacca ttccgcctcg
     5521 aaacccgtcg catgggatcg cttcgcgcca ccaggcacga cttattcctt ccgtctggca
     5581 tcggtcagcg ccagcgcccg ggcatagact ttgcgtttgg ggaggccgag gtcggccgca
     5641 ataagcgctg acgcatcgcg cgcagaacgg gtttccatct cggcaatgag ccgcgcatcg
     5701 atctggtcgt ccgtaacggt ttcgccagcg ccatcgaccg gcgcgaccag aatgacgatc
     5761 tcgccgcgcg gcttttcaac gtcggcatag tggcgggtca gttccggaag agtgccacgc
     5821 cgcaccgtct cgaatttctt ggtcagttcg cgcgcgactg ccgccggacg atcgcccagg
     5881 acttccgaca ggtccgacag cgattcggcc agccgatgcg gcgcctcgaa caagaccagc
     5941 gttcccggca ctgcttccag cgcacgaatc cgccgccgcc gctccgtctg ccgcgaaggc
     6001 aggaagccct cgaagaagaa ccgatccgtc ggcagtccgg ccagaaccag tcccgccaga
     6061 accgcagagg cgcccggcag cgccgagacg gcgatgccct gggcgatcgc cgattccacg
     6121 aggcgaaacc ccgggtcgga taccagcggc gtgccggcgt cggacaccag cgccagcgcc
     6181 gcgccctcgt gcagccgcgc catcacgtgc ggccgcacca cctccgcgtt gtgctcatgg
     6241 tagctcatga tgggcgtggt gatgccgtag tgcgcgagca gagtcttggt gacacgcttg
     6301 tcctcggcca ggatcacatc cgccgccgcc agcgcctcca gcgcccgcag ggtgatgtcc
     6361 ttgaggttgc cgatgggtgt tgcgaccaga tgcaggcccg gcgcgagagg aagcgcttcg
     6421 gccttcaggc caaaaagggt gtaggaggca gtcatcccac gatggtgcag gtccaccgca
     6481 cctcatgcaa gcgagtggat cgcaaaaaga cgagttttgc cggattccct tcggctgaag
     6541 aaggccgctg ctcggaaaca attaaccctg gtcaaaatat ccggccgggg ctgcagggca
     6601 ccgttccagc gttaccctat cgttaatgtg ccgcatttta gctatgatgt gatttgaaag
     6661 ggttgcgtgt tcatgcgtga ccaggactga tgaggccacg gcgatgtccg taactgaccg
     6721 ctctccattc aaacttgtcg gccccagccg tcgccatgtt gcgggaggcc tggcggtttc
     6781 ggtcgctgcg ctggtggcag ggtgcgccga tgtcaacacg acgtttccaa gcgttgccac
     6841 gccaagcgga ccaacgaacc agccgaccgg caacaccatc ggcaacggcc cggttcgcat
     6901 cgcgctgatc ctgccgctgt ccgcgccgaa cggagccgtg cccgcgcagg ccctgcgaaa
     6961 cgcggccgaa atggccatcg ccgaattcaa cagccaggaa ctgaccattc tggtgaaaga
     7021 tgacaagggc acgcccgagg gcgcgcaagc ggccgcgcag gaagccctca gcgagggggc
     7081 ggaggcaatc cttggtccgc tgttcgctcc ggcggtatcg gcggcggcca gtgtggcacg
     7141 cggcgcttcg cggcccgtga ttgctttttc gaccgatgca tcggttgccg cgcgcggcgt
     7201 ctacctcttg agcttcatgc cgcagtcgga cgtacggcgc atcgtttcct tcgccacgca
     7261 acgcggcaag aagtccttcg ccgcgctgat cccgcagtcc gcctatggaa gcgtcgccga
     7321 agccgagttc gcgcaggccg tcacctcaag cggtgcgcgg ctggcgatgg tcgagcgcta
     7381 tcagcccaac aaggcctcga tcgatgccgc gatagccaag atcaaggcgg gggcacagca
     7441 ggtcgactcc cttttcattc ccgacgatgg caacgggctg ccgggcgtcg gggcggcgct
     7501 gcagggcgcg ggcatttcaa gccggtccat gcagctgctc ggcactggcg tctggaacga
     7561 ggcgcgcgtg ttcaggaccg ctgccctgtc aggagcatgg ttcgccgctc cggagaacgc
     7621 cggcttccag aatttcgccc agcgctaccg cagccgcttc aattctgatc caacacgtat
     7681 cgccacgctg gcctatgacg ccaccgccct gctcagcgct ctggtgcgca cgcagggcgc
     7741 ccagcgcttc tcggaaggcg tgctgacgaa tgcctccggg tttgcgggac aggacggcgt
     7801 cttccgcttc cggcaggacg gcacgaacga ccgcgggctg gcgatctatc agctgggcaa
     7861 tggctcggcg caggtcatcc agccggcgcc gcgcaccttc gccgcaggat cggcctgacg
     7921 tccgctcacg cggccagatc cgccacgacc gcgtccagaa ccgggaaccc ggccggcgtc
     7981 acgcgcagcc ggccggtcgt atcccttgct accagtcctt ccacttcgag aacggcgatg
     8041 cgcctttcgt ccagcggacg accgcgcagc cgttcgaatc gctttggatc gatcccttcc
     8101 gctagcctga ggcccatcag caggaattca tccgcctcgt tctcctgtga caaccgttcg
     8161 gtggtgatgc agccgccgcc atcgcgctcc accaggctgc accatgcctc gggcgccttt
     8221 tcgttggcca tggccagccg cccgtccacc gtccagaggc ggccgtgcgc ccccgggccg
     8281 atgcccgcat actcgccgta gcgccagtac agcaaattgt gccggcattc ctcgcccggg
     8341 cgcgcgtggt tggagatctc gtaggccgga aggccctgcc ggtcacacac gtcctgcgtc
     8401 acgtcataga ggatgcgggc ttcctcggcc ccgggaacat gcagctttcc agcctgcgcc
     8461 agggcgtcga aggcagtatc gggctctatg gtgagctggt agagcgaaag gtgatgcggc
     8521 gcgaaggcga gcgcctccac caattcgctc cgccaggcct gcggcgtctg cccgggacgc
     8581 gcgtagatca gatcgaagga gaatcgctcg aacacgctgg atgcgacggc gatcgccgcc
     8641 agcgcctcgg ccacggaatg gaggcgtcca aggaacttca ggtcggtatc gatcagcgcc
     8701 tggacgccga gcgatacccg gttgaccccg gcggcccgat agccgcgaaa ccggcccgcc
     8761 tcgacgctgg tcgggttggc ttcgagcgtc acttcaacgc tggaatcgat cgcccaatgc
     8821 tgtgcgatgg cgtcaaggat gcccccgaca gtttccggcc gcatgaggga cggcgtgccg
     8881 ccaccgaaga agatggacga tacaacccgc ccgggggcct gcgcggccca gccggcgact
     8941 tcgcggcgga aagcggcaag aaagcgctcc tcatcaatgc cgccgcgccg cacatgactg
     9001 ttgaaatcgc aataggggca tttcgaggcg cagaatggcc agtgcacata aacgccgaat
     9061 ccggcgtcga agggcggaag tgcgctcatc gaaagcaggc cgccgtcatg gccgcgatcg
     9121 cgcgcgcgcg gtgggaatgc ttcttcttct ccgccatcgg catctcaccg aaggtgcgga
     9181 tctcgcccag cggcacgaaa cacggatcat agccgaagcc ttccggaccg cgcggcggga
     9241 aggcgagggt tccgtgcacc cgcccttcga aaacctgctc gccgccgtcc ggccaggcca
     9301 ccacgatggc cgagacgaaa tgcgcccttg cgccactggc gtcatgcccg cgtcgctcca
     9361 gttcgaaacg gaggcgctcc atggcccggg tgaaatttcg cgggctgccg gcccagtccg
     9421 ccgtgaacag ccccggcgcg ccatccagaa gatcgacgca caggccggaa tcatcggcga
     9481 tggccggcag tccggttgcc acagccgcgg cccgcgcctt gatggccgcg ttctcggaga
     9541 acagcgtacc ggtctccgcc ggttccggca ggccgagcgc gccggaagac accacttcga
     9601 caccgaaggg ggctagcaga tccaccattt ctgccagctt gccggcgttg tgagtcgcca
     9661 gaaccacgcg cccggccagt cggcgacccg tcatcaggcg accgccatct tctgcagatc
     9721 gaccagacgg ccgatgccct tgccggcaag ttcgaggagc ttcagcagct cttcctgcga
     9781 aaacggtgcg ccttccgccg ttccctgcac ttcgacaatg ccgcccgaac cggtcatcac
     9841 gaagttcgcg tcggtctgcg cagacgaatc ttccggatag tccaggtcca gcaccggcgt
     9901 accgttgtag atgccgcagg aaacggcggc gacatgatcg cggagcggca ggttcttcac
     9961 catctcgcgc gccttcatcc aggccaggca atcatgcagc gccacccagc cgccggtgat
    10021 ggcggcggtc cgcgttccgc cgtccgcctg cagaacgtcg caatcgatga cgatctgacg
    10081 ctcgccgagc gcgctcagat cgacgaccgc ccgcagtgcc cggccgatca gtcgctggat
    10141 ttcctgcgta cggcccgaag gcttgccgga cgtcacttcc cggcgcgtgc gctccagagt
    10201 ggcgcgcggc agcatcgcgt attccgccgt gacccacccc ttgcccgaac cgcgcagcca
    10261 gggtggtccg cgctcttcca gcgaggccgt gcacaggaca tgggtctcgc cgaacttgac
    10321 caggcaggag ccctcggcat agcgggcaac gccgcgctcg agcgttacct tgcgcagttc
    10381 gtccggggca cgtttggatg gccgcattgt cgtctctcgt caaatgggac cggcgctcat
    10441 acgccgttgc gggcccgccc acaagcgtcg accgcccgct tgctgacgaa tgccggctcc
    10501 gggtatggtc gccaaaaccc ccacgtgcag gccatgactg ccgatctgtc caagccaagt
    10561 tccggtcgcc ccggcgacat tgacgagcgc tcccgcgaaa tcttccgccg gatcgtcgat
    10621 ggctatcttg ccacgggcga gccggttgga tcgcgccaga tctcgcgcca gcttgcggtg
    10681 cagctgtcgc cggcgtcggt gcgcaacacc atgcaggatc tggaggacct cggcctgatc
    10741 tacgcgccgc ataccagcgc cggacgcctg ccgacggaaa tcggcctgcg cctgttcgtt
    10801 gacgccatgc tggagatcgg ggacgtctcg acggatgaga aggcgcgcat cgaggccgaa
    10861 gtgcaggcct caccgggcgc gaagggccgc agtttcgagg atacgctcgc ggaagcctcg
    10921 cgtctcctgt ccggcctttc gggaggcgcg gggcttgtcg cggcgacaaa gggcagcaaa
    10981 cgctggaaac atatcgaatt cgtccggctc gatccggcgc aggccctgat cgtgctcgtc
    11041 ggcgaggatg ggagccttga gaaccggctt ctgccattgc cgcccggcct gccggcatcc
    11101 gccctgttcg aggcttcgaa ctacctcaat gcccgcctga gaggccgcgc gctcggcgag
    11161 gtgcgtgccg agatcgaggc gcagcgggcc gagatggaac gggaactcga cgcgctcacg
    11221 gcccggctgg tggacagcgg cctggccagt gccgccgaga gcatcgacgg agtgccgcaa
    11281 ctgatcgtgc gcggtcaggc gaacctgctg gagaacgtca ccgccgccga tgatctggag
    11341 cgcatccgcc ggctcttttc cgacctcgag gccaagaagg acgtcatcga cctgctgtcg
    11401 cgcgccgaga acgctgatgg cgtgcgcatc ttcatcggtt cggagaacaa gctgttttcc
    11461 ctgtccggct cggcgatgat cgcggcgccc ttccggaatg gccagcagcg catcgtgggc
    11521 gtgctggggg tcatcggtcc gacccggctc aactacgccc gcatcgtgcc gctggtcgac
    11581 tataccgcgc gggtggtgag ccggatgctg gatcgctgac atgaaaaggg cgcctgtgcg
    11641 gcgcccctca tgtcagcgtg accctggtac ggacactcac gcggcgatca ggcgctgcgg
    11701 tgcggcggcc accacatcgc gaccgacgtg gctctcgaac gtcttgaagt tcttctcgaa
    11761 catggcgatc agcttcttcg ccgtctcgtt gaaggcggcc ttgtcctgcc aggtctttac
    11821 cggctggagg atatgcggct cgacgccggg caccgattcg ggaaccgaga agccgaaata
    11881 aggatcgcgg cggaagttgg cgcgattgag cgatccatcg agcgccgccg tcagcaggcg
    11941 acgggtcacg cggatcggca tgcgacggcc aacgccggcc ttgccgccgg tccagccggt
    12001 gttgacgagc cagcaatcgg ccccgtgctt ggcgatgaga tcgcgcagca ggttcccgta
    12061 gacggacgga tggcgcggca tgaagggatg gccgaagcag gtcgagaagg tggcctgcgg
    12121 ctccgtaacg ccgcgttcag taccggcgac gacggcggtg tagcccgaca ggaagtgata
    12181 catcgcttcc gcgcctgtca gcttggcgat cggcggcatc acgccgaagg catcgcaggt
    12241 cagcatgatg atgttgcggg ggtgaccggc gcggccggtc ttggacgaat tcgagatgaa
    12301 gtgcagcgga taggcgcagc gggtgttctc ggtcttcgtc tcgtcgtcga agtcggcgat
    12361 gcgggtgtcc ggatcgaaaa cgacgttctc aagcaccgtg ccgaagcgct gggtggtcgc
    12421 atagatctcc ggttcggctt cgggcgagag gcggatcgtc ttggcgtagc agccgccctc
    12481 gaaattgaaa acgccgtcct ggccccagcc gtgctcgtca tcgccgatga ggacgcggtt
    12541 cggatcttgc gagagcgtgg tcttgccggt gccggaaagt ccgaagaaca gagcgacatc
    12601 cccgtccttg ccaacgttgg cggagcaatg catcggcatg acgttgttgg tcggcagcac
    12661 gaaattcagg taggtgaaga cgctcttctt catttcgccg gcataggccg tgccgccgat
    12721 cagcacgagc ttgcgggaca ggtcgatggc gatcacggtc tcgctcttgc agccgtggcg
    12781 cttggggtcg gccctgaagc ttggcaggtc gacgatcgtc atctccggca cgaaatcctt
    12841 caggtccgcg acgtcggggc gaatcaaaag gttgcggatg aagagggaat gccaggcgag
    12901 ctcggtgtag acgcggacac ggacgcggtt cctggggtcg gcgccgccaa tcaggtcctg
    12961 aaggaacagg tccttgccct tggcgtgggc cttgaaatcg gccagcagcg tgtcgaacgc
    13021 ctctggcgtc atggcctgat tctgatccca ccagaccgtg tccgcggtcg tggcatcgcg
    13081 cacggtgaac ttgtccttcg ggctgcggcc agtgtggata ccggtctcgg caacaatcgc
    13141 accacctttc aggatctgcg cctcgccgcg gcggatggac tcttcgtaga gggccggcgg
    13201 catgaaattg cagaggatcg tgcgcgggct gtcgaagcca aacgtattcg caccgaccgt
    13261 ggtgttgtgt ttgccgcttg tgaccataag tcagtccttt tttctgacgg acggaagctg
    13321 ctggagaggc cagggtcgaa gccctcgaag ccatttgccg cccaatgcgc accgtcccta
    13381 ggcgatccgg ccgaaaagac cgttaatcag gtggcatgcc cctaacagaa aatccggatt
    13441 tgccaatgcg caggaccgga aattggcggg agaatcgtat tctcccttcg cattagtcgt
    13501 gcaaaaccct caaattttag gtcagtcctc tggttgtctg atgtcaggtg tcagcgcgcg
    13561 cctgcttcgc cagatcagcg agaacgctcc gcaaggtcgc cccgtcgacg acccgctgcg
    13621 gaaagaccag gcgcaatgtc tggtccccga aggccaggtc cattccctca ggatcgattc
    13681 cggtggcgcg ccaggggccg gcaccgcctc cgcacagacg gctggcataa agccgcaccg
    13741 catccgcatg gtcggcgttc atatgggcga gggcgtccgc ttcgaggtcc accagcgcgt
    13801 ctgccccgga taggtccgtc atgacgtccg atgcaggtcc atcccaggcc cgggcgaacc
    13861 cgccgttcag gtagacgtgg cgaggcgcca gcctccagaa tgagaaatcc gggaagtcga
    13921 cataaagttc ggccttgggc tgacgcgcca ggaaccggcg ccgcacccgc gcgccttcgg
    13981 cactgtcgcg atcgatgcgg ccggcggtgg cgaccacggt caggcgcggg tgcgcgagcg
    14041 gatcgccctt gccgggctgg ctcaagagca acgagcagcg tccgtctgct tctatctgac
    14101 gtgtatggga tgccagtcga gagatcagga tcaggggcgc cccatccata tccgtcgcca
    14161 tcgacaccag cgaagcgaaa ggtgcaccat caccctgcag cgtcgcaagc gtaccggacc
    14221 gcgccgtccg cagaatcgcc ttggcctgcg gcaacgggtc aaaacccggc gccattttcg
    14281 cagctacctc cggatctaca gccatcgcaa cctcccgctt gtgccatgat ctggcccgcg
    14341 catccgcctt gcgcaatgcc atttttccgt ccgccacaat tcggcctgcg tttgttcact
    14401 agaaaacact gcctatattg aggtcgcggt tgccatcggc acccgtgcgt tcaccgcgtc
    14461 agtcccctga ggaactcatg ccaaccatcg ctctcgtcga cgacgatcgc aacatcctga
    14521 cctcggtttc catcgccctg gaggcggaag ggtaccgggt gcagacctac accgacggag
    14581 ccagcgcact ggacgggttg cggcagaacc cgcccgacat ggcgatcttc gacatcaaga
    14641 tgccgcgcat ggacggcatg gaactgctgc ggcggctgcg ccagaaatcg gacctgccgg
    14701 tcatcttcct cacctcgaag gatgaggaga tcgatgagct gttcgggctc aagatgggcg
    14761 ctgatgactt catcaagaag ccgttctcgc agcgcctgct ggtcgagcgc gtcaaggcgg
    14821 tgctgcgccg cgccggcgcg aaagacgttg ccgcgcccaa ggaggccgat gcccgcgcgc
    14881 tcgaacgcgg ccagttgcac atggaccccg agcgccatgc ctgcacctgg aagggccagc
    14941 ccgtcacgct gacggtgacc gaattcctga tcctgcaggc cctggcgcag cgcccgggcg
    15001 tcgtgaagag ccggaatgca ctgatggatg cggcctacga cgatcaggtc tatgtcgacg
    15061 atcgcaccat cgacagccac atcaagcggc tcagaaagaa gttcaaggtg gtggacgacg
    15121 actttgatat gatcgaaacg ctttacggcg tcggctaccg cttcaaggaa gcgtgacgcc
    15181 cagaacgcga ggaccgggtg ctcgatcgca atccgctgcc agaagccagc gacagggatg
    15241 tcgcgaaccc ccggtccggg accaccggtg cggacagcgc gcaggctgcg cccgggccgg
    15301 tgcatcgcct gtctcgcgcg ttggccctgc gcctctcctc gagcgtggcc cgacgcatcg
    15361 ccctgctcaa tctcgccggc ctgctggccc tgctcgtagg cttcctgtgg ctgatccaga
    15421 cgcgagacag cgtgatcgac gctcgcgtgc agagcctttt cacgcaggcc gagatcattg
    15481 cctccgccgt cgcggcctcc gcctcggtcg agacagatgc aatcaccctc gatccggaca
    15541 agctgttgca gctccaggtt ggcgaaggca cgtctggtgc cgcggacgac atgcgctcgg
    15601 ccatcgagtt ctcgatcaac cccgagcgcg tcgcgcctgt gctgcgccgg ctggtttcgc
    15661 ccacgcgcaa ccgggcgcga atcttcgacc gcgacggcat catgctgctc gacaccaggg
    15721 cattttactc cgccggcgag atcgcccggc tcgatctgcc gcccgaaccg gaggaattca
    15781 cgctgttcga gcgcagctgg agcgcgatca agcgccgttt cgggcgcatg gacctgccgg
    15841 cctacgagga aaccggcaat gccagggcct accaggaggt cggcagggcc ttgctcggcg
    15901 cggcgggaag cgtcgtgcgc gtcagcgcca acggccagac catcgtctcc gtggctgtcc
    15961 ccgtgcggcg gttccgctcc gtgcagggcg cgctgatgct gaccacccag gaaggcgaca
    16021 tcgaccgcat catagcggaa gagcggttct ccatcctcat ggtgttcatc attgccgccg
    16081 gcgtcatgct ggtgctgtcg ttcctcctcg ccgggaccat cgccgagccg ctgcgcaagt
    16141 tggccgaggc ggcggaccgg gtgcggcgcg gcaccagggc gcggcaggaa attccggatt
    16201 tttccggccg ggccgatgag atcggccatc tcgccggcgc gctgcgtgac atgacccggg
    16261 ccctctacaa ccgcatcgaa ggcaccgaga ggttcgcggc ggatgtggcc cacgaactga
    16321 agaaccccct gacctcgctg cggtccgcag tcgaaacgct gcccctggca cgaaccgacg
    16381 aggcgcggtc ccgcctgttg tcggtgatcc agcacgacgt gcgccgcctc gatcggctga
    16441 tcaccgacat atcttcagcg tcgcgcctcg atgccgaact ggcgcgcgcc gaggccagcc
    16501 cggtcgacat cgctctcctg ctcgagaccg tcgtggccat ggcgaatgac gtgcgccggg
    16561 acgacggggt cacggtcagg ctgcaggtca tgccggagtc gcggcgcatt ccgggggccc
    16621 tcgtggtcaa cgggcacgat tcgcgactgg ttcaggtgtt caacaacgtc atcgacaacg
    16681 cgcgatcctt ttcgccgccc ggcgggacgg tggacgtccg ctgcgatgca agcgcggacg
    16741 ccgttcagat aaccatcgat gacaccggac ctggcattcc gccccatgcc ctgacccgga
    16801 tcttcgagcg gttctacacg gatcgcccca accagggctt cggacagaac tccggtctcg
    16861 gcctgtccat ctcccagcag atcgtcgagg cgcatcacgg tacgatccgc gccgagaacc
    16921 gcctcgatgg cgacggccgc acgctgggtg cccggttcac agtgactttg ctgcgggagg
    16981 ccgcgtgagc attgccggca tcacgctcca cgccggctgc gtggtgatcg gcgaaaaggc
    17041 tgttctgctg accggcaagt caggcgccgg caagtcctcc ctggcgcgcg cgctggtcga
    17101 cacggccgtc gcggaggggc ggtttgccgc gatggtcgcc gatgatcggg tcctcataag
    17161 ccggcacggc gaccggctgg tggcccgccc acatccgcgc atcgccgggc ttcaggaaat
    17221 ccgcggtttc ggcatcgtcc ggggaccgtt cgagccggcg gcggtcattg ccttggtcct
    17281 ggaactgtcc gcagggcgac aaccgcgcat gccggaggcg acaggggaaa cggcacatct
    17341 gctcggcatg agcgtgccct ccctgcggct ctacggcaat cgcccggtgg ccgaaaatgc
    17401 cgcgatcacc cgaattcgcc tgcgaactgc aacggtcttt tgacataggg gcacgacttt
    17461 tctcggtttg ccccttgaac ttttcttcgc gccgcacaaa atgcgcccct ttcgggagcc
    17521 gggcctcgat ccagaaggat cgccggactc caatggtcgg aacatgatcg gaatggttct
    17581 cgtgagccac ggccgccttg ccaacgagtt caaggcggcc atggaacacg ttgtcggccc
    17641 ccagaagcag gtggagaccg tctgcatcgg gcccgaagac gatatggaag cccggcgcct
    17701 ggaaatcgtt gaggcgatcc atgcggtcaa ttccggctcc ggcgtcgttg tgttgaccga
    17761 catgtttggc gggacgccat caaacctcgc gatttcggct atgaacggta cgggcgtcga
    17821 ggtggtggcc ggcatcaacc tgccgatgct ggtgaaactc gcgagcgtgc gcgatgcatg
    17881 ttgtctggcc gacgccgtcg tgcaggcgca ggaggccggc aagaaataca tcaacgtggc
    17941 cagccgggtg ctcgcgggca aatgaacgac gagccctgcc ccgatacaat catccctgaa
    18001 ggcgcgctgg cccgcgcaat gccgatcgtc aaccgcaagg ggctgcacgc acgcgcctcc
    18061 gcgaaattcg tgcaatgcgc agaacggttc gagtgcgaca tccaggtgca tcgctgcggc
    18121 gagaccgtcg gcggcacttc catcatgggc ctgctcacgc tcgccgcggc gaaaggcacg
    18181 accatcacgg tgacggcgcg aggctcggac gcggaagcag ccctggatgc gctggcggaa
    18241 ctgctggcga atcgcttcgg cgaagacgag tgagccggca caaccccggc ggaatggcga
    18301 cataaagatt tctttatatc tttattgccc tgccggtccg gggctgttat aggcgcgtcc
    18361 aatcccaaca gcaggacgca ctcctatgga atccatcgtc aaggacatgt cgctcgccgc
    18421 cttcggccgc aaggaaatcg agctggctga atccgaaatg ccgggcctga tggcgacccg
    18481 cgccgagtat ggcgccgcgc agccgctgaa gggcgcgcgc atcgcgggct cgctgcacat
    18541 gaccatccag accgcagtgc tgatcgagac actgaaggcg ctcggcgccg acatccgctg
    18601 ggtgtcctgc aacatctact cgacgcagga tcacgccgcc gccgccatcg ctgcggccgg
    18661 catccccgtc ttcgcctaca agggcgagac gctgaccgaa tactgggact acacggcgaa
    18721 gctgttcgac tggcacggcg gcggcatgcc gaacatgatc ctcgatgatg gcggcgacgc
    18781 caccatgttc gtgcatcttg gcctgcgcgc cgagcagggc gatgcggcgt tcctggacaa
    18841 gccgggatcc gaggaagaag agatcttctt cgccctgctg aagaagaagc tggcggagaa
    18901 gccgaagggc tggttcgcgg aactggccaa gtccatccgc ggcgtctccg aggagacgac
    18961 cacgggcgtg aaccgtctct accagttcgc cagggagggc aagctgctgt tcccggcgat
    19021 caacgtcaac gacagcgtca ccaagtcgaa gttcgacaac ctctacggct gccgcgagtc
    19081 gctggtggac gccatccgcc gcggcaccga cgtgatgctg tcgggcaagg tcgccttcgt
    19141 cgcaggcttc ggcgacgtcg gcaagggttc cgccgcgtcg ctgcgccagg ccggctgccg
    19201 cgtcatggtt tcggaagtcg acccgatctg tgccctgcag gcggcgatgg aaggctacga
    19261 ggtcgtcacc accgaggacg ccctgccgcg cgccgacatc ttcgtcaccg ccaccggcaa
    19321 caaggacatc atcaccgtcg accacatgcg ggcgatgaag gatcgcgcca tcgtctgcaa
    19381 catcggccat ttcgacaacg aaattcaggt ggccgcgctg cgcaacttca agtggaccaa
    19441 catcaagccg caggttgacg agatcacctt ccccaagggc aaccgcatca tcctgttgtc
    19501 ggaaggccgg ctggtgaacc tcggcaacgc catgggccac ccctccttcg tcatgtcggc
    19561 ctccttcacc aaccagacga tcgcccagat cgagctgtgg accaaccccg gcaagtacga
    19621 gaagaaggtc tacaccctgc ccaagcacct cgacgaaaag gtcgcgatgc tgcatctggc
    19681 caaggtcggc gcgaagctga cgaagctgcg gccggaccag gcctcgtata tcggcgtgcc
    19741 ggaaaacggc ccgttcaagc cggatcacta ccgctactga agacggcgcg ggcgcagcct
    19801 gcgaccaatc gactcaacca gcgcgcgttc ccgacgggga cgcgcgtttt ttctttgtta
    19861 accacttcct gacggagtca gccgccgatt
//
LOCUS       DAOMXN010000023        19789 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_1614_length_19789_cov_11.392622, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000023 DAOMXN010000000
VERSION     DAOMXN010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 19789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19789
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1614_length_19789_cov_11.392622"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1355
                     /gene="urtB"
                     /locus_tag="PK812_03715"
     CDS             <1..1355
                     /gene="urtB"
                     /locus_tag="PK812_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HRJ68693.1"
                     /translation="TGDLSSALDGAALKVAPGGMTPVRINNRLRGAIEIALGQLTLQS
                     PDPKTRRMAALAIYKSRTAEGEAALGAALEREKDEDIRIVQRLALAAIAATAPGRTDS
                     DRLAAIAVLRDHGDREALAPLSLVASDAGPQVREAAASAAAGIERRLALWASAQNVWY
                     GLSLGSVLLLAAIGLAITFGVMGVINMAHGEMVMLGAYVTFVVQDLIRSVAPAWFNSS
                     LAIAIPAAFLVAGLVGVAIERLVIRHLYGRPLETLLATWGISLILQQTVRTVFGPTNR
                     EVGAPGFMSGSFQLGLLEITWGRLWIVLFALLVFGAVLALLRFTSIGLDMRAVTQNRR
                     MAASMGIRTGRVDALTFGLGSGIAGLAGVALSQIDNVSPNLGQSYIIDSFLVVVFGGV
                     GNLWGTLVAALSLGIANKFLEPLAGAVLGKIALLVFIILFIQKRPRGLFALKGRAVES
                     "
     gene            1369..2526
                     /gene="urtC"
                     /locus_tag="PK812_03720"
     CDS             1369..2526
                     /gene="urtC"
                     /locus_tag="PK812_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HRJ68694.1"
                     /translation="MAPPLMSRIDTRGRMFLAAIAIALVLVPILNLAVPAQSALHVPT
                     WAVSLMGKYLCFAILALALDLVWGYCGILSLGHGAFFALGGYAMGMHLMRQIGPRGVY
                     GHPVLPDFMVFLNWKELPWFWHGFDQFPFAMLMVGLVPGLLAFVFGWFAFRSRVTGVY
                     LSIITQALTYALMLAFFRNDMGFGGNNGLTDFKDLLGYPVQSAGMRVVLFCLTLMTLA
                     ACYVLARGVVTSKAGKVLVAIRDAESRTRFLGFRVEHYKLFVFTLSAVMAGVAGALYV
                     PQVGIINPSEFQPANSIEVVIWVAVGGRGTLVGAAIGAILVNLAKTLFTTGMLAPYWL
                     LALGALFLFTTLFLPKGVIGAFEAWQERRAARRAARGAEGKEALHGAASHG"
     gene            2504..3256
                     /gene="urtD"
                     /locus_tag="PK812_03725"
     CDS             2504..3256
                     /gene="urtD"
                     /locus_tag="PK812_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HRJ68695.1"
                     /translation="MALRLTDDILYLDGVSVAFDGFRAINELSLAVGPNELRAIIGPN
                     GAGKTTMMDIITGKTRPDTGTVLFDGTVDLTRLDEAAIANLGIGRKFQKPTVFDMHTV
                     EDNLRLALKADRRVRSTLFWSATASEDARLDAISEIIRLSDHRAFMAGSLSHGQKQWL
                     EIGMLLAQDPKLLLVDEPVAGMTDAETEATAELLRLIARDHAVVVVEHDMAFVRALDC
                     RVSVLHEGSVLAEGRLDTVSANKRVIEVYLGR"
     gene            3272..3967
                     /gene="urtE"
                     /locus_tag="PK812_03730"
     CDS             3272..3967
                     /gene="urtE"
                     /locus_tag="PK812_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HRJ68696.1"
                     /translation="MLKLEAIDLYYGAAQALRGISLEAEIGQITCVIGRNGVGKTSLC
                     RAISGQQPITRGKIWLDGRDAGALGPVERARAGIGLVPQGREIFPLLTVEENLRTGFA
                     PLRRGERHIPPEVFDLFPVLSSMLGRRGGDLSGGQQQQLAIARALVMRPRLLILDEPT
                     EGIQPSIIKDIGRAIRHLALQKTMAIVLVEQYFDFALEFADRFVLLDRGEVIERGDRN
                     RMGQADVRQRLAL"
     gene            3942..4805
                     /locus_tag="PK812_03735"
     CDS             3942..4805
                     /locus_tag="PK812_03735"
                     /inference="COORDINATES: protein motif:HMM:NF013899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HRJ68697.1"
                     /translation="MSGSALRFDAAPADHDLPPSVRVDARLDLALDAPAGTTRIARRH
                     EQGAFRLRFPKSHCTTPEVCLVNIAGGLAGGDRVSFRFDAGPDAGAQVSSAAAERIYR
                     SAGDVTRIETRLTLDDRARVLWLPQETILHGGARLDRRFAIDVAPSARLLFGEMLYFG
                     RRASGETFTAGHLRESWRLRLGPTLTYADELRITGEFAADILHPVALFEHVAVATLIL
                     CAQDADEALDPIRATLASRHGIEGGASNLGGAVVIRLAARDAAGLRQTVLGLAAVMAA
                     RLPLPLPRALS"
     gene            4815..5117
                     /locus_tag="PK812_03740"
     CDS             4815..5117
                     /locus_tag="PK812_03740"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008121015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HRJ68698.1"
                     /translation="MNLTPREKDKLLVAMAAIVARRRLERGVKLNHPEAIALISDFVV
                     EGARDGRSVADLMAAAATILTRADVMDGIAEMIHDVQVEATFPDGTKLVTVHNPIR"
     gene            5134..5703
                     /locus_tag="PK812_03745"
     CDS             5134..5703
                     /locus_tag="PK812_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018095673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HRJ68699.1"
                     /translation="MKRFYAILFALASGPALAHVGADADHAALAAGFWHPLGGADHSL
                     AMVAVGLVAARAGGRGRYGIPGAFLAAMTVGFVLGATRIAVVPAHEAMILASVIGLGL
                     ALALAHPLPAAAAGAAAALFGLAHGFAHGVEGTLQLGYGAGFVAATALLHCAGLLIGM
                     AMQAFRHPVLFRLAGAGFAVAGLALAAAR"
     gene            5707..6012
                     /locus_tag="PK812_03750"
     CDS             5707..6012
                     /locus_tag="PK812_03750"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012066609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HRJ68700.1"
                     /translation="MIPGEILTRPGDIELNAGRETCLLTVANTGDRPVQVGSHYHFFE
                     ANPALRFERDKAHGFRLDIPAGTAVRFEPGQTRDVRLVRMAGKREVYGFRQAVMGAL"
     gene            6009..7715
                     /gene="ureC"
                     /locus_tag="PK812_03755"
     CDS             6009..7715
                     /gene="ureC"
                     /locus_tag="PK812_03755"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018236416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HRJ68701.1"
                     /translation="MMAKLSRAIYADMFGPTTGDRIRLADTDLFIEVEKDFTTYGEEV
                     KFGGGKVIRDGMGQSQVTRAGGAVDTVITNVVILDHWGIVKADIAIRDGLIHRIGKAG
                     NPDIQPDVDIIIGPGTEIIAGEGKIVTAGAFDSHIHFICPQQIEEALAAGITSMLGGG
                     TGPAHGTLATTCTPGPWHIARMIEAADAFPMNLGFAGKGNASLPAALEEQVLGGACAL
                     KLHEDWGTTPAAIDCCLSVADALDIQVMIHTDTLNESGFVEDTIAAFKGRTIHAFHTE
                     GAGGGHAPDIMKVAGLPNVLPSSTNPTRPFTVNTIDEHLDMLMVCHHLDPSIAEDLAF
                     AESRIRKETIAAEDILHDIGALSMMSSDSQAMGRIGEVIIRTWQTADKMKKQRGPLAG
                     DGASDNARAKRYVAKYTINPAIAHGVSRHIGSVEAGKLADLVLWSPAFFGVKPDMVLK
                     GGVIAMATMGDPNASIPTPQPMHYRPMFGAYGKARTGSSLTFVSKAALDDGLAGRLAV
                     AKRLVPVENCRAIGKASMIHNGATPRIDIDPETYHVRADGELLVCEPASVLPMAQRYF
                     LF"
     gene            7741..8151
                     /locus_tag="PK812_03760"
                     /pseudo
     CDS             7741..8151
                     /locus_tag="PK812_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046824.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
     gene            8204..8869
                     /locus_tag="PK812_03765"
     CDS             8204..8869
                     /locus_tag="PK812_03765"
                     /inference="COORDINATES: protein motif:HMM:NF013858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HRJ68702.1"
                     /translation="MTDTKEFQRQHWFSPAFPVGGYAYSHGIEMAVAAGAVDGPESLA
                     AWIAGVMRLGSTRNDMILLAVSYNADGLEQWRALAELAHALAASSGRRLESAQQGAAF
                     LAHMHSAWPHPGLPVWPDAEPLPYPVAVAIAGKVHEQPLVPLLVGYGQAFAGNLLAAG
                     IRLAVIGQERAQAVLASYAAEIVALANDAAHGTLDSLGSMAFRSDLMALRHEALDGRL
                     FRS"
     gene            8892..9506
                     /gene="ureG"
                     /locus_tag="PK812_03770"
     CDS             8892..9506
                     /gene="ureG"
                     /locus_tag="PK812_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HRJ68703.1"
                     /translation="MYNGPLRVGIGGPVGSGKTTLVAALCQRLRTATDLAVITNDIYT
                     REDAEILNRLQALPQDRILGVETGGCPHTAIREDCSINLHAIAEMRRRFPGLDLIFIE
                     SGGDNLAATFSPDLADLTIYVIDVAQGEKIPRKGGPGITRSDLLVINKTDLAPHVGAD
                     LEVMAQDAARQRGQRPVVFSNLRTGQGVADIIAFIQRSGGLALR"
     gene            complement(9544..10320)
                     /locus_tag="PK812_03775"
     CDS             complement(9544..10320)
                     /locus_tag="PK812_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011996090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRJ68704.1"
                     /translation="MDMQIDGLRVLITAGAGGIGLEVARAFSREGAKVFVCDVDAAAL
                     QALATQDPTIRGVHCDVADRAAVARMFEEAVGWLGGLDVLVNNAGIAGPTARVEEVRP
                     EDWDRCLEVCLTSQFTCARLAVPHLKKSANASIINLSSAAGKHGFALRSPYAAAKWGV
                     IGFTKSLAIELGDHGIRVNAILPGLVKGDRIRRVFEAKAQQYGESFATIERGAFAATS
                     IKEYILPQQLADQMVFIASPRGRTISGQAIAVDGDTKMLM"
     gene            complement(10360..11076)
                     /locus_tag="PK812_03780"
     CDS             complement(10360..11076)
                     /locus_tag="PK812_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRJ68705.1"
                     /translation="MNYETRIKQAFDLTDTGGLESGTLSHRVYAHLSQLLVSGKLAPN
                     DKLSFRAVAESLGTSMMPVREAISRLVAEKALVVEPKKAAFVPVMSATGFRDITRVRI
                     IIEGAASAMAASSATQSDLQDIARCEIAFRNLEGDPRAVRQAAVAANQAFHFAVYRAA
                     HSGELLAIIERLWLRVGPVINLDLRENPERMKFGGAIRYHAALLDGIRQRDPQAARAA
                     LAADIQGAADFILSQGKLPE"
     gene            11178..12428
                     /locus_tag="PK812_03785"
     CDS             11178..12428
                     /locus_tag="PK812_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRJ68706.1"
                     /translation="MSDLIRPSRRGLIKGAGATALLAGVAMPAISHAQADAIRIGHLT
                     PRTGFLGPLGEYAVMAVDLATEEINAAGGILGRKLEVLKEDSVNPQTASTKAERMIER
                     DKVAAIVGEISSASCLTIAQVADRTKTLFINTGGNSDALRGKDCKRYMFHIESQNSMY
                     VKTAGRSLMQQGLITDKKIYSLTADYAFGHDLTSVAKRFFAQNKGNLVADDLVPTDAT
                     DFSAYLLKIRNAKPDLVISNLAGNQITNFLKQYAEFGLAHPVAGFGFDTALAWAAGKD
                     NFAGTWPVVWHHLIDTPKSKAFVAAFTKKYNKPPENQAWGDYMMMKILAQSITETKST
                     DSAKLMEHWEKGAKFDVLKTREGYFRASDHQMMHEMYTVEALKPSDVKNQWDLFKSSP
                     AVPGANESLEVIATQGDEAVCKFS"
     gene            12574..13443
                     /locus_tag="PK812_03790"
     CDS             12574..13443
                     /locus_tag="PK812_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020091907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRJ68707.1"
                     /translation="MLFVQQLLNGLLDGVYYLLIALGLSLIFSLGGIVNLAHGAFFAI
                     GAYLTIVFSPYIGFSGSLVASPVAVAMIGMLMERFLFRRFYRSDPILSLLLTFGLAMV
                     AEQALRWIFGAPPLSYSMPRELRGQIFIGDFVYSYYRTILLGIAIVCVAGIWLLLHKT
                     SFGRVVRAGVQNPDMVGALGISLQPYMTAVAALGIGLAGLAGVLLAPIYAVHPAMGQE
                     IITPAFVVCVIGGLGSFWGVVVAALLVGLVKGFMVAINYPAASIAAIYFMMLVVLLVR
                     PRGLFGERILRFE"
     gene            13448..15964
                     /locus_tag="PK812_03795"
     CDS             13448..15964
                     /locus_tag="PK812_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HRJ68708.1"
                     /translation="MRSYRPLLIAAIGLVILPIALNAIGLTWTSATEVVVFALATMAL
                     NILVGHTGLVSFGHGAWFGLGAYIAGLAQLHWFPGSFVGPILVALATVAVLSAGFGAL
                     ILRRRGVYFSLLTLALSAMLYTIAFRWTAVTGGENGLGGIKRPSWFGINLENSQTYYI
                     FVALIAFAVTYVLWRFHASPVGKTLVAIRENEQRARFVGYPTNTYKLIAFTLSATITA
                     LAGTLLLYQNRMTSAEPISVAFSGELLIMVVIGGMRSFLGPALGALFFILFREYLSTI
                     TEHWLLFFGLLFVGFILFSPDGLVGVYERATRPFRKIVTEAAAMSGRKAGASGRIPSH
                     LIRQGNGTGGALDVTGIAKSFGGIRAVKDVSFAVGDRTLHALIGPNGAGKTTAFNLVS
                     GMFTPDQGRVVLDGRDISGFNPERITEAGIGRSFQITNLFPTLTVEENIRLAVQARHP
                     NRMNVWRNAHALDQVAADTAELMRWTGLAGAEQAEAGSLSYGGQRLLDMGLALATRPR
                     ILLLDEPLAGLAAAERTRVGDLIKALSADIPVLLVEHDIDRVFRLADRVTVMNEGEVL
                     VDGTVEDARSSQRVQDVYLGSGNHAVAAKPRETAALPDVLLSLESVNTFYGKSHILND
                     VTFDVRQNEVIALLGRNGAGKSTLLKTIIGIAPPRTGEIRLSSASIARLPSARIARAG
                     VGFVPQGRAMFAGMSVRDNLALGRHSRLTGEGRHWDEDKILEFFPRLKERMDTPADYL
                     SGGEQQMVAVARALSGDTRVLLLDEPFEGLSPAVTEELFEAFHKLRQELSIIIVDHNL
                     DLALAISDRTVALERGAVMHSGPSRDLRDDLDLRRKVLWL"
     gene            15990..16931
                     /locus_tag="PK812_03800"
     CDS             15990..16931
                     /locus_tag="PK812_03800"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020091909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HRJ68709.1"
                     /translation="MTKIAVVGTGLIGRAWATVFAAAGYEVALFDAHPEATRAAKTFV
                     ARSLREQEALGLVDDARAGAGRVHVAMSLAEALKGVALVQENGPETLEEKTKLFAEMD
                     RIAPRNAILASSTSFIPCSRFSEALAGRRRCLVAHPVNPPHLVPIVELAPAPWTDAAV
                     VQRAKRIYERAGQVPIVLRREVPGFVLNRLQAVLLAEAFRIVGSGVCSPQDLDKTIRD
                     GLGHRWSFMGPFETIELNAPGGIPDYAARYGASLERMASDTLMDDPFGPRTVDTIMGQ
                     WPGAQEKERIATLSRWRDRRLTALKAHKRTAVRKPSL"
     gene            16945..17877
                     /locus_tag="PK812_03805"
     CDS             16945..17877
                     /locus_tag="PK812_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HRJ68710.1"
                     /translation="MAKPNKVIITCAVTGAIHTPSMSPYLPVTPQEIADAAIGAAEAG
                     AAIVHLHARDPVNGKPDQRPEAFEPFLRVIKQRSDCVVNITTGGAPTMLVQERVGPAA
                     HFAPEVASLNMGTMNFGLYPMLNRFKDFKHDWEKPYLEGSTDRIFRNTFADIEFILRT
                     CADNGTRFEIECYDIGHLYTLAHFVDRGLVKAPFFVQSVFGLLGGIGPHPEDVAHMKR
                     TADRLFGTDYRWSVLGAGRNQLPIAAMAAAMGGNVRVGLEDSLWIGAGKLATSNAEQV
                     RAARQIIEGLGLEIATPDEARQILELKGKDRVNF"
     gene            18010..18861
                     /locus_tag="PK812_03810"
     CDS             18010..18861
                     /locus_tag="PK812_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010659336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HRJ68711.1"
                     /translation="MKIALLQMNSVASVEANITQLTELTERAVAQERPDMIVPPEAWN
                     WFGGGAKEKFANADETRGGRSYKAAQALARKHKIWVHAGSLSERNPNGKVNNVTYVFD
                     REGREVARYCKIHMFDITAPDGSRYAESATAQPGREIVTYQAEGLTFGCAICYDLRFP
                     ELFQALAMRGAEVIVLPAAFTLQTGKDHWDVLIRARAIETETYMCAAGMTGPHTSDDG
                     STRFTYGHSLIADPWGHIICKASDGVGYTAGRIDKAFIKKCRQQIPVLEHKVVGTPQM
                     PLRSAAE"
     gene            complement(18887..19780)
                     /locus_tag="PK812_03815"
     CDS             complement(18887..19780)
                     /locus_tag="PK812_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HRJ68712.1"
                     /translation="MPTSCIADEDRPGSPKAGMVWVARGTFRMGSDAAWPEERPPRPV
                     TVDGFWIDRHHVTNAQFARFVAATGYVTFAERRDPRSGVANGSLVFSQPRDIRDLQDI
                     GQWWRLVPGANWRHPEGPGSGIAERQHHPVVHVAYEDAAAYAAWAGRELPTEEQWEFA
                     ARGGREGALYTWGDERDTGEAPLANRWHGTFPLVNTGSRGFRGTSPVGCFPANGYGLY
                     DMAGNVWQWTRSVWTDDHAAPQDSSGSTGERVIKGGSFLCAPNYCMRFRPAARQPGEP
                     GSSTSHIGFRTVHAGAPPPGG"
BASE COUNT         3236 a         6600 c         6466 g         3487 t
ORIGIN      
        1 ggaccggcga tctgtcgagt gccctcgacg gcgccgccct gaaggtcgcg ccgggtggaa
       61 tgacccccgt gcgcatcaac aaccgcttgc gcggagcgat cgagatcgcg cttgggcaac
      121 tcaccttgca gtcgccggac cccaagacgc gccgcatggc ggcgctcgcc atctacaagt
      181 cgcgcaccgc agagggcgaa gcagcactgg gtgccgctct ggagcgcgaa aaggacgagg
      241 acatccgcat cgtccagcgg ctggcactgg ctgcgatcgc agcaaccgcg cccggccgga
      301 ccgattcaga ccggcttgcg gcgatcgccg tgttgcgcga ccacggagac cgggaagcgc
      361 tggctcccct tagtctcgtg gcttccgatg ccggaccgca ggttcgcgag gcggcggcga
      421 gcgccgcggc cgggattgag cgtcgccttg ccctgtgggc gagcgcccag aacgtctggt
      481 acggcttgtc gcttggttcg gttctgctgc tggccgcgat cggcctcgcc attaccttcg
      541 gcgtcatggg cgtcatcaac atggcgcacg gcgagatggt catgctcggc gcctatgtca
      601 cattcgtcgt ccaggacctc atccggtcgg tggcgccggc atggttcaac tcctcgctgg
      661 cgatagcgat cccggcagca ttcctggtcg ccggcctggt cggcgtggcg atcgagcgcc
      721 tggtgatccg gcacctttat ggcaggccgc ttgagacgct gctggccacc tggggcatct
      781 cgctgatcct gcaacagaca gtccggacgg ttttcgggcc gaccaatcgc gaagtcggcg
      841 ctccgggttt catgtccggc tcatttcagc tcggcctgct tgagatcacc tggggacggc
      901 tctggatcgt gctgtttgcg ctgctggtgt ttggcgccgt gctcgccttg ctgcgcttca
      961 cctccatcgg gctggacatg cgcgcggtga cgcagaaccg caggatggcc gcatcgatgg
     1021 gcatccgcac agggcgcgtg gatgcgctca ccttcgggct tggctccggc attgccgggc
     1081 ttgcgggcgt cgccctgtcg cagatcgaca atgtcagccc caatctcggc cagagctaca
     1141 tcatcgacag cttcctcgtc gtggtgttcg gcggcgtcgg caacctctgg ggtacgctgg
     1201 ttgccgccct ctcgctcggc atcgccaaca aattcctgga gcccttggcg ggtgctgtcc
     1261 tcggcaagat cgccctgctc gtcttcatca tcctgttcat ccagaaacgg ccgcgcggac
     1321 ttttcgcgct caagggccgt gccgtcgaga gctgaggccg cccgcatgat cgcccctccc
     1381 ctgatgagcc ggatcgacac gcgcgggcgc atgtttctcg cggcgatcgc cattgccctc
     1441 gtgctcgtgc cgatcctcaa tctcgccgtt ccggcgcagt ccgccctgca tgtgccgacc
     1501 tgggcggtca gcctaatggg caagtatctc tgcttcgcca tcctagccct ggcgctggac
     1561 ctggtgtggg gctactgcgg catcctgagc ctcggtcatg gcgcgttctt tgccctgggc
     1621 ggctatgcga tgggcatgca cctgatgcgg cagatcggtc cgcgcggcgt ctatggccat
     1681 cccgtactgc cggacttcat ggtgttcctg aactggaagg aattgccatg gttctggcac
     1741 gggttcgacc aattcccctt cgccatgctc atggtcggcc tggtccccgg cctgctggcc
     1801 ttcgtgttcg gctggttcgc cttccgaagc cgggtgaccg gtgtctatct ctcgatcatc
     1861 acgcaggcgc tcacctatgc gctgatgctc gcgttcttcc gcaatgacat gggcttcggc
     1921 ggcaacaacg gcctgacgga tttcaaggac ctgctcggct atcccgtgca gagcgccggc
     1981 atgcgggttg tcctgttctg cctgacgctg atgacccttg cggcctgcta cgtcctggct
     2041 cgcggcgtcg tcacgtccaa ggcgggcaag gttctggtcg ccatacgcga tgccgaaagc
     2101 cgcacacgct tcctcgggtt ccgtgtcgaa cactacaagc tcttcgtctt cacgctgtca
     2161 gccgtgatgg ccggggtcgc gggcgcgcta tacgtgccgc aggtcggcat catcaatccg
     2221 agcgagtttc agccggccaa ttcgatcgag gtcgtcatct gggtcgcggt cggcgggcgg
     2281 ggcaccctcg ttggcgcggc gatcggggca atcctcgtaa atctggccaa gacgctgttc
     2341 acgaccggca tgctggcgcc atactggctg ctggcgctcg gcgcgttgtt cctgttcacg
     2401 acgctgttcc tcccgaaggg cgtcatcggt gccttcgaag cctggcagga acggagggcc
     2461 gcgcgccgcg ctgcgcgtgg agccgaagga aaggaggccc tccatggcgc tgcgtctcac
     2521 ggatgacatc ctgtatctgg acggcgtctc ggtcgccttc gatggctttc gggcgataaa
     2581 cgagctttcg ctcgcggttg gtccaaacga gctccgtgcc atcatcggtc ccaatggcgc
     2641 cggcaagacg accatgatgg acatcatcac cggcaagacc cgccccgata ccggcaccgt
     2701 gcttttcgat ggcaccgtcg acctgacgcg cctcgacgag gcggcgatcg ccaatctggg
     2761 catcgggcgg aagttccaga agccgaccgt cttcgacatg catacggtcg aagacaacct
     2821 gcggctagcg ctcaaggcag accggcgggt gcgaagcacg ctgttctggt cagccacggc
     2881 gagcgaggac gcgcgtctcg atgccatcag cgagatcata cgcctgtcgg atcaccgcgc
     2941 gttcatggcc ggaagtctga gccacggcca gaagcagtgg ctcgaaatcg gcatgcttct
     3001 ggcgcaggac ccaaagctgt tgctcgtcga cgagcccgtc gccggaatga ccgatgccga
     3061 gaccgaagcc accgccgagc tgctcaggct gatcgcgcgt gaccacgcgg ttgtggtggt
     3121 cgagcacgac atggccttcg tgcgcgcgct cgactgccgc gtcagcgtgc tgcacgaagg
     3181 ttccgtcctc gccgaaggcc ggctcgacac cgtgagcgcc aacaagcgcg tcatcgaggt
     3241 ctatcttgga cggtaggaat acgaagcgat gatgctcaag ctcgaagcca tcgacctgta
     3301 ctatggcgcg gcgcaagccc tgcgcggcat ttcgcttgaa gcggagatcg gccagatcac
     3361 ctgcgtgatc ggtcgcaacg gcgtgggcaa gacatcgctc tgtcgcgcta tctccggcca
     3421 gcagccgatc acccggggca agatctggct ggatggccgt gacgccggcg cgctgggtcc
     3481 cgtggagcgt gcccgggccg gcatcggact ggttccgcag ggtcgcgaga tttttccgct
     3541 gctcacggtc gaagagaacc tgcgcaccgg ctttgctcca ctgcgtcgcg gcgagcggca
     3601 cattccgccg gaggtgttcg acctctttcc ggtgctcagc tcgatgctgg gccggcgcgg
     3661 cggcgacctt tccggcgggc agcagcagca gctggccatt gcccgcgcgc tggtgatgag
     3721 gccgaggctg ctcatcctgg atgagcccac ggaaggcatc cagccctcca tcatcaagga
     3781 catcggccgc gcgatccgcc atctggcctt gcagaaaacg atggccatcg tccttgtcga
     3841 gcagtacttc gacttcgccc tcgagtttgc tgatcgcttc gtgctactcg accgtggcga
     3901 agtcatcgag cgcggagaca ggaaccgcat ggggcaggca gatgtccggc agcgccttgc
     3961 gctttgacgc agcccccgcc gatcacgacc tgccgccgtc ggtgcgcgtg gatgcccggc
     4021 tggatctggc gcttgatgcg ccggccggga cgacccggat cgcccgacgt catgaacagg
     4081 gcgccttccg cctccgcttt ccgaagtcgc actgcaccac gccggaagtc tgcctggtca
     4141 acatcgccgg cggactggcg ggcggggatc gtgtgtcttt ccgcttcgat gccgggccgg
     4201 atgccggcgc gcaggtgagc agcgccgccg ccgagcgcat ctaccgctcg gccggcgatg
     4261 tgacgcggat cgaaacgcgg ctgacgctcg acgatcgcgc ccgcgttctc tggttgccgc
     4321 aggaaacgat cctgcacggc ggggcgcggc tggaccgtcg attcgcgatt gacgttgcac
     4381 catcggccag gctgctgttc ggggaaatgc tgtacttcgg caggcgcgct tccggagaaa
     4441 ccttcactgc gggacatctt cgggaaagct ggcgcctcag gctcggacca accctgacct
     4501 atgcggacga actgcggatc acgggggagt tcgctgcgga catcctgcat cccgtcgccc
     4561 ttttcgagca tgtggcggtc gccacgctga tcctgtgcgc gcaggatgcg gacgaagcgc
     4621 tggacccgat ccgcgcgacg ctggcatcgc ggcatgggat cgagggtggc gcgtcgaatc
     4681 tcgggggcgc ggtcgtgatc cggctggccg cccgggacgc ggccggcctg cgccagaccg
     4741 tactgggcct cgcggccgtg atggccgcgc ggttgcccct gcccctgcca cgtgccctga
     4801 gctgagtacg acggatgaac ctgacccccc gtgagaaaga caagctgctg gtcgccatgg
     4861 cggcgatcgt ggcccgccgc cgcctggaac gcggcgtgaa gctcaatcat cccgaagcga
     4921 tcgcgctgat ttcggacttt gtcgtcgaag gtgcgcggga cgggcgttct gtcgccgatc
     4981 tcatggccgc ggccgcaacc attctgaccc gcgccgacgt gatggacggg atagccgaga
     5041 tgatccacga cgtgcaggtc gaggcaacct ttccggacgg gacgaagctc gtcaccgttc
     5101 acaatccaat ccggtgatcc acggagaagt gtgatgaagc gtttctatgc gatcctgttc
     5161 gcccttgcct cgggtcctgc ccttgcgcat gtcggggcag atgccgatca cgctgccctg
     5221 gcggctgggt tctggcatcc gctgggcggc gcggaccaca gcctcgccat ggttgccgtt
     5281 ggcctcgtgg ccgcgcgcgc cggtggccgg ggccgctacg ggattccggg cgcctttctg
     5341 gcggcaatga ccgtaggttt tgttctcggc gccacgcgga tcgccgtcgt gccggcccat
     5401 gaagcgatga tcctggcatc ggtcattggc cttggattgg cgctggccct cgcccatccg
     5461 ctgccagccg cggctgccgg cgcagccgcc gcgctgttcg gcctcgcgca tgggtttgcc
     5521 catggcgtcg agggcacgct tcaactcggc tatggcgcgg ggttcgtcgc ggccaccgcg
     5581 ctgctgcatt gtgccggcct gctcattggc atggccatgc aagcgttcag acatccagtg
     5641 cttttccgcc tcgcgggcgc aggcttcgcg gtcgctggcc tggcacttgc cgcggcgagg
     5701 tgatccatga ttcccggcga aatcctgacc cgacccggcg acatcgaact caacgccggc
     5761 cgcgagacct gcctgctgac cgtcgccaac accggcgacc ggccggttca ggtcggctcg
     5821 cactaccact tcttcgaggc gaacccggcg ctgcgcttcg agcgggacaa ggcgcacggc
     5881 ttccggctcg acatcccggc cggcacggcc gtgcggttcg agccggggca gacccgcgac
     5941 gtccgcctcg tgcggatggc agggaagcgg gaggtctatg gcttccggca ggctgttatg
     6001 ggggcattgt gatggcgaag ctttcgcgcg cgatttacgc ggacatgttc ggccccacca
     6061 ccggcgatcg catccgcctg gccgataccg acctcttcat cgaggtcgaa aaggatttca
     6121 ccacctatgg cgaggaagtg aagttcggcg gcggcaaggt catccgcgat ggaatgggcc
     6181 agtcgcaggt gacccgggcc ggcggcgcgg tcgacaccgt catcaccaac gtggtgatcc
     6241 tcgaccactg gggcatcgtg aaggcggata tcgcgatccg cgacgggctg atccaccgca
     6301 taggcaaggc cggcaacccg gacatccagc ccgatgtcga catcatcatc ggtccaggca
     6361 ccgagatcat cgcgggcgaa ggcaagatcg tcacggcggg cgcgttcgat tcccacatcc
     6421 atttcatctg cccgcagcag atcgaggagg cgctggccgc cggcatcacg tcgatgctcg
     6481 gcggggggac cggtcccgcg cacggcactc ttgcaacgac gtgcacgccc ggcccctggc
     6541 acatcgcgcg catgatcgag gccgctgatg cctttccgat gaacctcggc ttcgcgggca
     6601 agggcaatgc ctcgcttcct gcggcgcttg aggaacaggt cctgggaggg gcctgcgcgc
     6661 tcaagctgca cgaggactgg ggcacgacgc cagccgcgat cgattgctgc ctttcggtcg
     6721 ccgacgcgct cgacattcag gtgatgatcc ataccgacac gctgaacgag agcggcttcg
     6781 tcgaggatac gatcgccgct ttcaaggggc gcaccatcca tgcctttcac accgaaggcg
     6841 cgggcggcgg acatgcgccg gacatcatga aggttgcagg gctacccaat gtcctgccga
     6901 gttccaccaa ccccacgcgg cccttcacgg tgaacaccat cgacgagcat ctcgacatgc
     6961 tcatggtctg tcatcacctc gatccatcga tcgcggaaga cctggcgttc gccgaaagcc
     7021 gcattcgcaa ggaaaccatc gcggcggagg atatcctgca cgacatcggc gccctctcga
     7081 tgatgagctc cgacagccag gcgatgggca ggatcggcga ggtaatcatc cggacctggc
     7141 agaccgccga caagatgaag aagcagcggg ggccgctggc cggcgacggc gccagcgaca
     7201 atgcgcgcgc caagcgctat gtcgcgaaat acacgatcaa tccggcgatc gcgcacggcg
     7261 tttcccggca catcggctct gtggaggccg gcaaactcgc cgacctcgtg ctctggtccc
     7321 cggccttctt cggcgtgaag ccggacatgg tcctcaaggg cggcgtgatc gccatggcga
     7381 ccatgggaga cccgaacgcc tcgattccga cgcctcagcc catgcattat cgtccgatgt
     7441 tcggcgccta cggcaaggcg cggacaggca gcagcctgac gttcgtcagc aaggcggcgc
     7501 tggatgacgg ccttgccggt cgcctcgccg tggccaagcg gctggtgccg gtggaaaact
     7561 gccgcgccat cgggaaggca tcgatgattc acaacggggc tactccgcgc atcgacatcg
     7621 atccggaaac ctaccacgtt cgggccgatg gcgagttgct ggtctgcgag ccggcatcgg
     7681 tgctgcctat ggcgcagcgc tacttcctgt tctgaacgca cggagaaaag gaacgtcatc
     7741 gtgctgcgcg caacgcgtgt catcgctgcc ggcgcctggg acggcgaacc ggccgaccgg
     7801 gtcgtcctgg agttcgacga ccgccaccgc cgccggattc acatgagcgg cgagggcggg
     7861 ctgtccttcc tcctcgatct ggcgagtgcc gtggcgttgc gctcgggcga tggccttgtg
     7921 cttgaagacg gccgcctggt cgaggtcgtc ggcgcggcgg aggatctgtg cgagatcgca
     7981 gccacggatg cggcgcactt cctcaggctc gcctggcacc tgggaaaccg ccacctcccg
     8041 gtgcaggtcg gcgatggctc cctgcggatt cggcgcgacc gcgtgatcga ggagatggtg
     8101 gcgggtcttg gcgggcgcat gcgcgccgtg aaagccccct tcgatccgga gacgcgcgcc
     8161 attcacgggg ctcacgacca ccacggccat gccgcgcacc ggcatgactg acacgaagga
     8221 gttccagcgc cagcactggt tctcgcccgc ctttccggtg ggcggctatg cctattccca
     8281 tggcatcgag atggcggtcg cagccggcgc ggtcgatggg ccggagagcc tcgccgcctg
     8341 gattgcaggc gtgatgcggc tgggctcgac gcggaacgac atgatcctgc tggcggtgag
     8401 ctacaacgcg gacggccttg agcaatggcg cgcgctggcc gagctcgccc atgccctggc
     8461 cgcgagttcc ggccgacggc tcgagagcgc ccagcagggc gcggctttcc tcgcgcacat
     8521 gcacagcgcc tggccgcatc ccggcctccc ggtctggcct gatgccgaac cgctgcccta
     8581 tccggtcgcg gtcgccattg ccggcaaggt ccatgagcag ccgcttgtgc cgttgctggt
     8641 tggctacggg caggctttcg ccggcaatct cctggcggcg ggcattcgcc tcgccgtgat
     8701 cggccaggag cgggcgcagg ccgtgctggc gtcctatgcg gcggaaatcg tcgcgctcgc
     8761 caacgatgca gcccatggca ccctcgactc tctcggcagc atggcgttcc gttccgatct
     8821 gatggccctg cgccatgagg cactggacgg acgcctgttc cggtcctgac ccctcaatgg
     8881 agacctgacc catgtacaat ggcccgcttc gcgttggcat cggagggccg gtcggctctg
     8941 gcaagacgac gctcgtcgcg gccctgtgcc agcgcctgcg caccgcaacc gatctggcag
     9001 tgatcaccaa cgacatctac acacgcgagg acgccgagat cctaaaccgg ctccaggccc
     9061 tgccgcagga ccgtatcctg ggtgtggaga ccggcggctg cccccacacg gccatccggg
     9121 aggactgctc gatcaacctg cacgccatcg ccgagatgcg tcggcgcttt ccgggcctcg
     9181 acctgatctt catcgaatcg ggcggagaca acctcgcggc aaccttttcc cccgatctcg
     9241 cggacctgac gatctacgtc atcgacgttg cgcaaggcga gaagatcccg cgcaagggcg
     9301 gtcccggcat cacccggtcg gacctgcttg tcatcaacaa gacggacctg gcgccgcatg
     9361 tgggtgctga cctggaggtg atggcgcagg acgcagcccg ccaaaggggg cagcgcccgg
     9421 tggtattcag caacctgcgc accgggcaag gggtcgcgga catcatcgcc ttcatccaga
     9481 ggagcggcgg cctggccttg cgctagaacg ccgtgtccgt gctccggcac cgcagcccag
     9541 ccgctacatc agcatcttgg tgtcgccatc cacggcaatg gcctggccgg agatcgtccg
     9601 cccgcgcggc gaggcaatga agaccatctg gtccgccagc tgctgcggca ggatgtattc
     9661 cttgatcgag gtggcggcga aggccccgcg ctcgatggtt gcgaagctct cgccatactg
     9721 ctgcgccttg gcttcgaaaa cgcggcggat gcggtcgcct ttgaccaggc ccggcagtat
     9781 cgcgttgacg cggatgccat ggtctccaag ctcgatcgcc agcgacttgg tgaagccaat
     9841 cacgccccac tttgccgcgg catagggtga gcgcagcgcg aagccatgct tgcccgcggc
     9901 cgacgacagg ttgatgatcg aggcattggc gctcttcttc agatgcggaa cggccagtcg
     9961 cgcgcaggtg aactgactcg tcaggcatac ttcgaggcag cggtcccagt cttccggcct
    10021 gacctcctcc acgcgcgcgg tcggcccggc gataccggca ttgttgacca gcacgtccag
    10081 gccgccaagc cagccgacgg cctcttcgaa catccgcgcc acggccgcgc ggtccgccac
    10141 gtcgcaatga acgccccgga ttgtcggatc ctgagtcgcc agcgcctgaa gcgccgccgc
    10201 atcgacatcg cagacgaata ccttcgcgcc ctcccggctg aaggcgcgcg ccacctcaag
    10261 gccgataccg ccggccccgg cagtgatgag aacgcgaagt ccgtcgatct gcatatccat
    10321 taggcttctc cgatgtctcg atacatgccg ccactgccgc tactcgggca gcttgccctg
    10381 cgaaagaatg aaatccgccg ctccctgaat gtcggcggcc agcgccgcac gcgccgcctg
    10441 cggatcgcgc tggcggatgc cgtccagaag cgcggcgtga taccggatag cgccgccgaa
    10501 cttcatgcgt tcggggtttt cgcgcagatc gaggttgatg accggcccga cccggagcca
    10561 cagcctttcg atgatggcca gcaactcgcc ggaatgggct gcccgataaa ccgcaaaatg
    10621 aaacgcctga ttggccgcca ccgccgcctg acggacagct ctgggatcgc cttccaggtt
    10681 ccggaaagcg atttcacagc gcgcaatgtc ctgcaaatcc gactgcgttg cggaagacgc
    10741 agccatcgcg gacgccgccc cttcgataat gatgcgcacg cgggtgatgt cgcggaaccc
    10801 ggtcgcactc atcacgggaa cgaaggcggc cttcttcggc tcaacgacca gcgccttttc
    10861 cgcgaccagg cgggatatcg cctccctgac cggcatcatc gatgtgccga gggattcggc
    10921 caccgcacgg aacgacagct tgtcgttggg cgccagcttc cccgatacca gcagttgcga
    10981 gagatgcgca tagacgcggt gcgacagcgt cccggattca aggccgccgg tgtccgtcag
    11041 gtcgaaggcc tgcttgatgc gcgtttcgta attcatggct ggacaagtgc cttcccttgc
    11101 gtcacgataa ctgtgatcac agatggcagc aaggtccgag agagaccttc tgcgaggaaa
    11161 cagccaggga gaaagacatg agtgacctca ttcgtccgtc gcgccgcggt ctcatcaagg
    11221 gtgcgggcgc aaccgcgctg ctcgccggag tggcgatgcc ggcgatcagc cacgcgcagg
    11281 ccgacgccat ccgcatcggg cacctgaccc cgcgcaccgg ctttctcgga ccgctcggcg
    11341 agtacgccgt gatggcggtc gatctggcga cggaagagat caacgccgcc ggcggcattc
    11401 tcgggcgcaa gctggaagtc ctcaaggaag actcggtcaa cccgcagacg gcttcgacca
    11461 aggcggaacg catgatcgag cgcgacaagg tcgcggcgat cgtcggtgag atttcctcgg
    11521 cgtcctgcct caccatcgcg caggtcgcgg accgcacgaa gacgctgttc atcaacaccg
    11581 gcggcaattc ggatgcgctg cgcgggaagg actgcaagcg gtacatgttc cacatcgaaa
    11641 gccagaactc gatgtacgtg aagaccgctg gtcgttcgct gatgcagcag gggctgatca
    11701 ccgacaagaa gatctattct ctgaccgccg actatgcctt cggtcacgac ctgaccagcg
    11761 tcgccaagcg cttcttcgcc cagaacaagg gcaatctcgt cgccgacgat ctggtgccca
    11821 ccgacgccac cgacttctcg gcctatctgc tgaagattcg caatgccaag ccggacctcg
    11881 tgatctcgaa cctggccggc aatcagatca ccaatttcct gaagcagtat gccgagttcg
    11941 gcctggcgca cccggtcgcg ggcttcggct tcgatacggc gctggcctgg gccgccggca
    12001 aggacaactt cgccggcacc tggccggtgg tgtggcatca cctcatcgat acgccgaagt
    12061 ccaaggcgtt cgtcgccgcc ttcaccaaga agtacaacaa gcccccggaa aaccaggcgt
    12121 ggggcgacta catgatgatg aaaatcctcg cccagtcgat caccgaaacc aagtcgaccg
    12181 acagcgcgaa gctcatggag cactgggaaa agggtgccaa gttcgatgtg ctgaaaacgc
    12241 gcgagggcta tttccgagcg tccgaccacc agatgatgca cgagatgtac acggtcgagg
    12301 ccctcaagcc ctcggatgtc aagaaccagt gggacctctt caagtcctca cccgcggtcc
    12361 cgggcgccaa cgagagcctc gaagtcatcg cgacgcaggg tgacgaagcg gtgtgcaagt
    12421 tcagctgacg ctcctgcccc cgccctcccc cgctcgcggg agagggcggt gcatttccga
    12481 acaaagggaa accgtctctc acagggcgtt ccgttcatcg ctcccgttgc ctggcgagag
    12541 cgataacggg tacgtcacgg ccaagatcgc gacatgcttt tcgtccaaca attgctgaat
    12601 ggcctgctcg acggggtcta ttacctcctg atcgcgctcg gcctgtcgct gatcttctcg
    12661 cttggtggga tcgtgaacct cgcgcacggc gcgttcttcg ccatcggcgc ctatctgacg
    12721 atcgtctttt ccccctacat cggcttttca ggctcgctgg tggcctcgcc ggtggcggtc
    12781 gcgatgatcg gcatgctgat ggaacggttc ctgttccgtc gcttctaccg gtcggacccc
    12841 atcctgtcgc tattgctgac cttcgggctg gcgatggtgg cggaacaggc gctgcgctgg
    12901 atcttcggtg cgccgccgtt gtcctactcg atgccccgcg agctgcgcgg gcagattttc
    12961 atcggcgact tcgtctattc ctattaccgg acgatcctgc tgggcatcgc gatcgtctgc
    13021 gtggcgggca tctggctgct gctccacaag acgtccttcg gccgcgtggt gcgggcaggc
    13081 gtgcagaatc cggacatggt gggcgctctc ggcatctcgc tgcaacccta catgacggcg
    13141 gtggccgcgc tgggcatcgg gctggccggc cttgccggcg tcctgctcgc gccgatctac
    13201 gccgtgcatc cggcgatggg gcaggagatc atcacgccgg ctttcgtcgt ctgcgtgatc
    13261 ggcggtctcg ggtcgttctg gggtgtcgtg gtggcggcgc tgctggtcgg ccttgtcaaa
    13321 ggcttcatgg tcgccatcaa ctatcccgcc gcctcgatcg ccgcgatcta tttcatgatg
    13381 ctggtggtgt tgctcgtgcg cccgcgcggg ctgttcggcg aacgcatcct gaggttcgag
    13441 tagcccgatg cgcagctatc gtcccctgct gatcgccgcc atcggtctgg tgatcctgcc
    13501 catcgcgctc aatgccatcg gcctgacctg gacctcggcc acagaggtcg tcgtcttcgc
    13561 cctcgccacc atggcgctga acatcctggt cggccacacc ggcctcgtct cgttcggcca
    13621 tggcgcctgg tttggccttg gcgcctacat cgccgggctg gcccaactcc attggttccc
    13681 cggatcgttc gtggggccca tcctggtggc gctcgccacg gtggcggtgc tgtccgccgg
    13741 attcggcgcc cttatcctga ggcggcgcgg tgtctacttc tcgctgctga ccctggcact
    13801 ttcggcgatg ctctacacca tcgcctttcg ctggaccgcc gtgacgggcg gcgaaaacgg
    13861 ccttggcggg atcaagcgtc caagctggtt cgggatcaac ctggagaatt cgcagaccta
    13921 ctacatcttc gtggcgctca tcgcctttgc cgtcacctat gtcctgtggc gcttccatgc
    13981 ttcgcccgtc gggaaaacgc tggtggcgat ccgcgagaac gagcagcgcg cgcgcttcgt
    14041 cggctacccg accaacacct acaagctgat cgccttcacg ctttcggcga ccatcacggc
    14101 gctcgccggc acgctgctgt tgtaccagaa ccgcatgaca tcggcggagc cgatctcggt
    14161 cgccttctcc ggagagctgc tgatcatggt ggtgatcggc ggcatgcggt cctttctcgg
    14221 tccggcgctc ggcgcgctgt ttttcatcct gttccgcgag tatctctcga ccatcaccga
    14281 gcactggctg ctgttcttcg gactcctgtt cgtggggttc atcctgttct cgcccgacgg
    14341 tctcgtcggg gtctatgaac gggcgacgcg cccgttccgc aagatcgtga cggaagcggc
    14401 cgccatgtcc ggtcgcaaag ccggcgcctc gggccgcatc cccagccatc tgatccgcca
    14461 gggcaacggc accggcggcg ccctggatgt gaccggcatc gccaaatcct tcggcggcat
    14521 tcgggcggtc aaggacgtca gcttcgcggt tggggaccgg acccttcacg cgctgatcgg
    14581 tccgaacggg gcaggcaaga ccacagcttt caatctggta tcgggcatgt tcacccccga
    14641 tcagggacgc gtcgttctgg acgggcgcga catcagcggg ttcaatccgg aaaggatcac
    14701 cgaagcgggg atcggccgct cgtttcagat caccaacctg ttcccgaccc tcacagtgga
    14761 agaaaacatc cgcctggccg tgcaggcccg tcatccgaac cggatgaatg tctggcgcaa
    14821 tgcccatgcg ctggatcagg tcgctgccga taccgccgag ctgatgcggt ggaccgggct
    14881 ggcgggcgcg gagcaggcgg aggccggttc gctctcctat ggcggacagc gcttgctgga
    14941 catgggcctg gcgcttgcca cacgcccgcg catcctgctg ctggacgagc cgctggccgg
    15001 gctagccgcc gccgaacgca cccgtgttgg cgatctcatc aaggccctgt cggcggacat
    15061 tccggtgctg ctggtcgagc atgacatcga ccgggttttc cggcttgccg accgggtgac
    15121 ggtgatgaac gagggcgagg tgctggtcga cgggacggtg gaggatgcgc gctcctcgca
    15181 gcgggtgcag gatgtctacc ttggctccgg caatcatgcc gtcgcggcaa aaccgcgcga
    15241 aaccgccgcc ctgcccgatg ttctgctgtc gctggagtcc gtcaacacgt tctacggcaa
    15301 aagccacatc ctcaacgacg tgacgttcga tgtccgccag aacgaggtga tcgcgctcct
    15361 tgggcgcaac ggcgcgggca aatccacgct cctgaagacc atcatcggca tcgcgccgcc
    15421 gcgcaccggc gagattcgtc tgtccagcgc ctccatcgcg cgcctgcctt ccgccaggat
    15481 cgcgcgggcc ggcgtcgggt tcgtgccgca gggacgggcg atgttcgcgg gcatgagcgt
    15541 gcgcgacaat ctggcgctcg gccgccattc acgcctgacc ggcgagggcc ggcactggga
    15601 cgaggacaag attctcgaat ttttcccgcg cctcaaggag cgcatggaca cccccgccga
    15661 ctacctctcc ggaggcgagc agcagatggt ggcggtggcg cgggcactgt cgggcgacac
    15721 gcgcgtgctg ctcctggacg agccgttcga aggcctgtcg ccggcggtga cggaagagct
    15781 gttcgaggcg tttcacaagc ttcggcagga gctttcgatc attatcgtgg accataacct
    15841 ggatctcgcg ctggccattt cggaccgtac cgtggcgctg gaacgcggcg ctgtcatgca
    15901 cagcggaccg tcaagggacc tgcgcgatga cctcgacttg aggagaaaag tgctttggct
    15961 gtaactccag tttcgcggaa acggtggcca tgacgaagat cgctgttgtt ggaaccgggc
    16021 tgatcggccg ggcctgggcc acggtttttg ccgctgcggg gtacgaggtg gcgctgttcg
    16081 atgcgcatcc cgaggcgacg cgcgcggcca agaccttcgt ggcccggagc ttgcgcgaac
    16141 aagaagcgct cggactcgtc gacgacgccc gggcgggcgc cggccgcgtg catgtcgcca
    16201 tgagcctcgc ggaggcgctg aagggggtgg cgctggtcca ggaaaacggt cccgaaacgc
    16261 tcgaagaaaa gacaaagctc ttcgccgaaa tggaccgaat cgcgccgcga aacgccatcc
    16321 tcgcctcgtc cacctcgttc atcccctgct cgcgcttttc ggaggcgctt gccggacggc
    16381 ggcgctgcct ggtcgcccat ccggtcaacc cgccccatct ggtgcccatc gtcgaactgg
    16441 cgcccgcgcc ctggacggac gccgccgtcg tgcagcgcgc caagcgcatc tacgagcggg
    16501 ctggtcaggt gccgatcgtg ctgcggcgcg aggtgccggg cttcgtgctc aaccgcctgc
    16561 aggcggtgct gctggcggaa gccttccgca tcgtgggctc cggcgtctgc tcgccgcagg
    16621 atctcgacaa gaccatccgc gacggtcttg gccatcgctg gtccttcatg ggaccgttcg
    16681 agacgatcga gctcaacgcg ccaggcggca ttccggacta cgccgcccgc tacggtgcct
    16741 cgctcgaacg gatggcgagc gacacgctga tggacgatcc tttcggcccg cgcacggtcg
    16801 acacgatcat ggggcaatgg ccgggggcgc aagagaagga gcgtattgcg accctctcgc
    16861 gctggcgcga cagacgtctc accgcgctca aggcgcacaa gcgtaccgcc gtcaggaaac
    16921 catcactgtg aaagggggcc gcaaatggcc aagccaaaca aggtcatcat cacctgtgcc
    16981 gtgaccggcg cgattcacac gccgtcgatg tcgccttacc tgccggtgac gccgcaggag
    17041 atcgccgacg cggccatcgg ggcagcggag gccggcgcag cgatcgtgca ccttcacgcg
    17101 cgcgatccgg tgaacggcaa gccggaccag cgcccggaag cattcgaacc gttcctgcgc
    17161 gtcatcaagc agcgctccga ctgcgtcgtg aacatcacca cgggcggcgc gccgaccatg
    17221 ctcgtgcagg agcgcgtcgg cccggcggcc cacttcgcgc ccgaggtcgc gagcctgaac
    17281 atgggcacga tgaatttcgg cctctatccg atgctgaacc gcttcaagga cttcaagcac
    17341 gactgggaaa agccctacct ggaagggtcc accgaccgca tcttccgcaa caccttcgcc
    17401 gacatcgaat tcatcctgcg gacctgcgcc gacaacggca cgcggttcga gatcgagtgc
    17461 tacgacatcg gccatctcta cacgctggcg catttcgtcg accggggatt ggtgaaggcg
    17521 ccgttcttcg tgcagtccgt gttcggcctg ctcggcggca tcggtccgca tccggaggat
    17581 gtcgcgcata tgaagcgcac cgccgaccgg ctgttcggaa cggattaccg ctggagcgtg
    17641 ctcggcgccg ggcgcaacca gttgcccatc gccgcgatgg cggcggccat gggcggcaac
    17701 gtgcgcgtgg ggctggagga ttcgttgtgg attggcgcgg gcaagctcgc gacctccaac
    17761 gccgagcagg tcagggcggc gcgccagatc atcgaaggcc tcggcctgga gatcgcgacc
    17821 cctgatgagg cccgccagat tctcgaactg aagggcaagg accgggtgaa tttctagggc
    17881 aggtttcgac ctggccactc cgtcgcggag cacaggccgc ctgccccggc gggtgggaag
    17941 cgcggcgtgc cccattgagg aaccgacggt tcgcgggcag tatgcggact ttcaagacaa
    18001 ggacctgcca tgaagatcgc cctgcttcag atgaattcgg tggccagcgt cgaggccaac
    18061 atcacccagc tgaccgagct tacggagcgg gctgtggcgc aggagcggcc cgacatgatc
    18121 gttccgccgg aggcgtggaa ctggtttgga ggcggcgcga aggagaaatt cgccaacgcc
    18181 gacgagacgc gcggggggcg ctcctacaag gcggcgcagg cgctggccag gaaacacaag
    18241 atctgggtcc acgccggctc gctgagcgag aggaacccga acggcaaggt caacaacgtc
    18301 acctatgtct tcgaccgcga gggccgggag gtggcgcgct actgcaagat tcacatgttc
    18361 gacatcaccg cgccggacgg ctcgcgctac gccgagagcg cgacggccca gccggggcgc
    18421 gagatcgtca cctaccaggc cgaggggctg acgttcggct gcgccatctg ctacgatctg
    18481 cgctttcccg aactgttcca ggcgctggcg atgcgcggcg ccgaggtcat cgtgctgccg
    18541 gcggccttca cgctgcagac cggcaaggat cactgggacg tgctgatccg cgcccgcgcc
    18601 atcgagaccg agacctacat gtgcgcggcg ggaatgaccg gcccccacac ctcggacgac
    18661 ggctcgaccc gcttcaccta cggccactcg ctgatcgccg acccgtgggg gcacatcatc
    18721 tgcaaggcgt ccgacggcgt cggatacacc gccgggcgga tcgacaaggc gttcatcaag
    18781 aagtgccgcc agcagatccc ggtgcttgaa cacaaggtcg tcggcacccc ccagatgccg
    18841 ctcaggagcg ccgcggaata agcggccggg cgaagaaccc gccgatctac ccgcccgggg
    18901 gcggcgcccc ggcgtgcacg gttcggaacc cgatgtggga cgtgctgctg cccggctcgc
    18961 cgggctggcg cgcggccggg cggaagcgca tgcaatagtt cggcgcgcag aggaaggagc
    19021 cgcccttgat gacgcgctcc ccggtcgatc cggatgagtc ctggggcgcg gcatggtcgt
    19081 cggtccagac cgaacgggtc cactgccaga cgttgccggc catgtcgtaa agcccgtatc
    19141 cgttggccgg gaaacagccg accggcgagg tgccgcgaaa tcccctgctg ccggtgttga
    19201 ccaggggaaa cgtgccgtgc cagcgattgg ccagcggcgc ctccccggtg tcgcgttcat
    19261 cgccccaggt gtagagcgcg ccctccctgc cgccgcgcgc ggcgaattcc cattgctcct
    19321 ctgtcggcag ttcccgaccg gcccaggccg cataggcggc ggcgtcctcg taggcgacat
    19381 gcaccacggg atgatgctgg cgctcggcga tgccgctccc cggcccttcc gggtggcgcc
    19441 agttggcgcc gggaacgagg cgccaccatt gcccgatgtc ctgaagatcg cggatgtctc
    19501 tcggctggct gaagaccagc gagccgttgg cgacgcccga gcggggatcg cggcgttccg
    19561 cgaaggtgac gtagccggtg gcagcgacga agcgcgcaaa ctgcgcgttg gtgacgtggt
    19621 ggcgatcgat ccagaacccg tcgaccgtca ccggacgcgg cgggcgctcc tcgggccagg
    19681 cagcatccga ccccatgcgg aaggtcccgc gcgccaccca caccatgccc gccttcggac
    19741 tgccgggcct gtcttcgtcc gcgatgcagg aggtcggcag cgccggctc
//
LOCUS       DAOMXN010000024        19370 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_1654_length_19370_cov_11.458038, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000024 DAOMXN010000000
VERSION     DAOMXN010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 19370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19370
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1654_length_19370_cov_11.458038"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="PK812_03820"
     CDS             complement(<1..385)
                     /locus_tag="PK812_03820"
                     /inference="COORDINATES: protein motif:HMM:NF013624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C15"
                     /protein_id="HRJ68713.1"
                     /translation="MRLLITAFGPFPGVPKNPSEALARRIAADRRLKAAGIETAVRIF
                     PTEYAAVTRDIPEILAEVRPACVLMLGVASRRRHLSVEKRGANRVSRLHPDASGARPP
                     HLLLKPGAPAFRLARAPLGSIVVAAL"
     gene            complement(386..1378)
                     /gene="meaB"
                     /locus_tag="PK812_03825"
     CDS             complement(386..1378)
                     /gene="meaB"
                     /locus_tag="PK812_03825"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HRJ68714.1"
                     /translation="MSAIPAAPSVEDLAVRVRSGDRAALARAITLIESKRLDHQALAR
                     NLVQQLLPLTGRAIRLGLTGVPGVGKSTTIDALGMMLIGQGLKVAVLAVDPTSSRTGG
                     AILGDKTRMSRLSVEPNAFIRPSPAGATLGGVAARTREAMLLCEAAGFDVVIVETVGI
                     GQSETAVCDMTDFFLVLLLPGGGDELQGIKKGVIELADMIAINKADGDALPRARITAT
                     EYSAALNLLTPDGAPWKPPVVLTSGLNGEGLDALWGNVRRHRALLHDSGDLQARRARQ
                     QVKWMWSMLDDRLRLRLRTDAKVRARLPEIERSVFDGALSATLAADEVAVMLGL"
     gene            1580..1840
                     /locus_tag="PK812_03830"
     CDS             1580..1840
                     /locus_tag="PK812_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68715.1"
                     /translation="MTIRFAPKILTAAAVLGAALFAAAPASAQYYGYHGPRVAAPPPT
                     VYHGPHYGVHPHHRPWKRWAWRHHHRPPPPWAYGPPRYGYQW"
     gene            1962..2312
                     /locus_tag="PK812_03835"
     CDS             1962..2312
                     /locus_tag="PK812_03835"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018042340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - diacylglycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HRJ68716.1"
                     /translation="MLKLYNAFLNTCAGFAHGLRTERAIQQEAFLLAIGLPLAFWIGA
                     TGWERLALVLALVAVLCVEFLNTAIEKLCDHVTPERHDTIKAIKDMGSAACFCAQAGC
                     GLIWLFAAWQRFWP"
     gene            complement(2356..2844)
                     /locus_tag="PK812_03840"
     CDS             complement(2356..2844)
                     /locus_tag="PK812_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HRJ68717.1"
                     /translation="MNMTHYMELLAVNQPWNLLIFMAVPVILAETVAITELYILYARA
                     YDGVARQLNRLASIVGGIYFAGVFVYLMATAVLPITQAGAWRGPADYIAVFAYLAGVV
                     PLGMIALIDLKLVAKDKDEHARMGLHALWVAVFLVVAHIAMVFGMLDPTVLGGKAGGH
                     AH"
     gene            complement(3354..4508)
                     /locus_tag="PK812_03845"
     CDS             complement(3354..4508)
                     /locus_tag="PK812_03845"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HRJ68718.1"
                     /translation="MQTAIGVISGTSMDAIDVALVRTDGEQAVRTGPARSYPYHRLLR
                     EELLAVIDNPYRAEHLPMPELDDAVADAHSNAVMRFMAENAIAREQVDLVGFHGQTVF
                     HAPQHKLTRQLGDGPRLARRLGIPVVWKFRHADVAAGGEGAPFVPLYHRALAAGLQKP
                     LIILNLGGVANITWLDGEGIVAMDTGPASALLDDFVQKRLGKPFDENGALAASGSVNV
                     AVLMKLLDNPFFDRPPPKSLDRNEFHQGAALVEGLSDADGAATLAAFTVEATLKCLPL
                     LASRPGRLLVTGGGRRNSTFMKLFAARLGIPVEPVEAVGWDGDALEAQCFAYLAVRSR
                     RDLPLSLPTTTGVPQPMPGGEWLDAKGNAIARPEPPPVIEEPAEEPPAQD"
     gene            complement(4579..5580)
                     /locus_tag="PK812_03850"
     CDS             complement(4579..5580)
                     /locus_tag="PK812_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HRJ68719.1"
                     /translation="MPTNHMAVLASRPVGRIRKSDFEVKEVPVPSIGEGEILVRVKVI
                     SLDPAMRGWVQDVKSYLPPVQIGEVMRAGGAGIVEASNNPRFKVGDAVTGVFGVQEYA
                     VSDGKAVAKVDTRLAPLETWIGGLGMPGMTAYFGLLDVGQPKAGETVVVSAASGAVGS
                     IVGQIAKIKGCRVVGIAGGPEKCRYVVEELGFDACVDYKAGNLRQQLKAACPNGIDVN
                     FENVGGEILDTILPMMNLFGRVAVCGLISEYNATEMPEGVKNFRVILVKRLRVQGLIV
                     FDWAKRYPEGVADLVRWRDEGKLKLKEDVREGGVAKFAETVDLLFSGGNFGKLVLKV"
     gene            5724..6620
                     /gene="argB"
                     /locus_tag="PK812_03855"
     CDS             5724..6620
                     /gene="argB"
                     /locus_tag="PK812_03855"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HRJ68720.1"
                     /translation="MSKFPLPDVHVQAEVISQALPHMQRYDQQTVVIKYGGHAMGDKA
                     AQEDFAEDIVLLEQAGVKPIVVHGGGPQIGRMLEKLGIKSEFQAGLRVTDKATVEIVE
                     MVLAGAINKQIVGWIGAEGGKAIGLCGKDGSMVTAKKLTRTMVDPDSSIEKVVDLGFV
                     GDPHKVDRSVLDAVLKSELIPVIAPVAIGEDGETYNVNADTFAGAIAGAMRAKRLLLL
                     TDVPGVLDKNKQLIPELTVNDCRKLIADGTVTGGMIPKIETCIYAIEKGVEGVVIMNG
                     TVPHAVLLELLTDHGAGTLITR"
     gene            6716..7732
                     /gene="corA"
                     /locus_tag="PK812_03860"
     CDS             6716..7732
                     /gene="corA"
                     /locus_tag="PK812_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HRJ68721.1"
                     /translation="MLQPATRPAPSFTPSGPGVVNAAVYSGGRRVRDVAIDEAGTWAR
                     KQDHIVWIGLYEPDDDLLKRVQMQFGLHDLAIEDASHAHQRPKIEAYGDGHFIVTRTA
                     QIVDGRAAFGETHIFVGKGYVISVRHGASTSYATVRARCEAAPNSLSHGEDYILYAIL
                     DFIVDNYMDVLETMHDDVEAIEDIVLSEALTQEQIERLYNLRRDLLRMRNAVTPLVDV
                     CRRLEVTDSVYISENMRVLFRDVTDHIRRVQEEIESLREVLAFAFETSLMTGQAQQTA
                     ISRKLSSGAAILAVPTAIAGIYGMNFKHMPELESPYGYYVVIAVILTLCSILFWRFRK
                     NGWL"
     gene            complement(7737..9134)
                     /locus_tag="PK812_03865"
     CDS             complement(7737..9134)
                     /locus_tag="PK812_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007802008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HRJ68722.1"
                     /translation="MSRETAIAAVHAEFDSGAFRDELARRIAIPTESQNPERAQALTD
                     YLTQQLAPAFSAIGFSTRVLAHPRAKGPFLYAERIEDPQALTVLGYGHGDVIRGLDEG
                     WSEGLSPWVLTERGDRWYGRGVVDNKGQHAINLAAQRAVIAARGKLGFNAKWIIEMGE
                     ETGSPGLREVCSENRDLLRADLLIASDGPRLAAGRPTVFMGARGAYQLDLVVEARKGG
                     HHSGNWGGLLSNPGIQLAHAIATIVGPTGQIRVPELVPEGGIPESVRRALAGCTIDSG
                     PDGPAIEPDWGEPGLSAAEKVFGWCNFEVLAFETGTPRTPVNAIPPRAWARCQLRHVV
                     GIDPARIVPAIRRHLDRQGLTMVRVETTRDDNFPATRLDPDHPAVRFAAASLERTTGK
                     APAVLPNLGGALPNDIFSDMLGLPTVWVPHSYPGCSQHAPDEHVPRALVREALGVMAG
                     LYWDIGQASKSDLLG"
     gene            complement(9154..10125)
                     /locus_tag="PK812_03870"
     CDS             complement(9154..10125)
                     /locus_tag="PK812_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014132787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRJ68723.1"
                     /translation="MMVPVLAIDALQVHFAARQRWFGPRLPPVRAVDGVSLTVARGET
                     LGLVGESGCGKSTLANAALGLVRATAGSVRIDGQEVVGADREMLRAIRARAQMVFQDP
                     ALSLNPRLSVGAAIGEPLLVRGLAKGEELARRIADLLRAVGLNPEHAARFPHQFSGGQ
                     RQRIGIARALALSPDLLICDEAVSALDVSVRAQVLNLLARLQRERGLSYLFVSHDLAV
                     VHHVCDRVAVMYLGRIVEEAPRDALFTNPRHPYTRALMQAVPEPDPVVQRARARLPLA
                     GEPPSPSNVPSGCAFHSRCPLATGICRAERPDLSPRGGPGRVACHHA"
     gene            complement(10122..11120)
                     /locus_tag="PK812_03875"
     CDS             complement(10122..11120)
                     /locus_tag="PK812_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ68724.1"
                     /translation="MAAPVLSIHDLTLRIAGHAVVDRLSLEVGEREVVALVGESGCGK
                     SLTALAAMRLLPPAVEAVSGGIAVGGQDVLRLSETRMRDLRGRQLSIIFQEPAASLDP
                     LTTVGAQIAEAVMAHEAVGKAQALERARSMLNAVGIADADRRLRQYPFELSGGMCQRV
                     MIAIALICRPRLLIADEPTTALDVTIQAQILDLMKTLVVDRGASILLITHDMGVVADM
                     ADRVAVMYAGRIVETGPAARIFAAPAHPYSALLLASIPRLSRPSKTDLPAIEGAVPTA
                     QDMPAGCRFSTRCPLVRDRCRREAPPLTPEGQGAVACWRTADVAAFSATLAPGMAP"
     gene            complement(11124..11990)
                     /locus_tag="PK812_03880"
     CDS             complement(11124..11990)
                     /locus_tag="PK812_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007768732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ68725.1"
                     /translation="MIVALREGRAVLNRLPLAARIAALVLALFLLAALAAPWLSPLDP
                     ARQTLLARLRPPGFTTAAGVTYWLGTDDLGRDLYARLLHGARLSIAIAVLSVVLSCVV
                     GTAVGMAAAWFRGVTEIVLMRLVDIMMSIPAILLAVLSVAVLGPGLLNLVIVLGLTRW
                     PRYARVAYGQTLQSATLPYVKAAELAGVRAPRLLLRHILPNIAGPLLVVVTAEFGLMI
                     LFEAGLSFLGLGIQPPDPSWGSILSVGRNYLERAWWMTAFPGACLFMLVLSVNVLGDA
                     LRDHLDPRAQGR"
     gene            complement(11987..12910)
                     /locus_tag="PK812_03885"
     CDS             complement(11987..12910)
                     /locus_tag="PK812_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007768723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ68726.1"
                     /translation="MTRYILYRLLQAVIATLGVLTIVFVVMRLAGDPTLLLAPDNASR
                     EQIEALRVALGFDRPLHVQYFAYVGDLLRLNLGESVVQRAPAIDILMLRLPYTVSLAL
                     AALTVAIGIGVPVGVIMAVRPDHWLARLLGALTLAGQSMPTFLSGILMIMVFAVTLGW
                     LPSSGADSASGIVMPAIALGALSMATFARMARIAVLDELSKDYVRACRARGLSIWTAV
                     FRHVLRNAALPLVSVAALEIGNLLAGSIIVENVFAWPGVGQLALQAIQSRDFLLVQAI
                     VLFISVTYVATSLAADLVYAWIDPRIVLARS"
     gene            13061..13633
                     /locus_tag="PK812_03890"
     CDS             13061..13633
                     /locus_tag="PK812_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007610348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRJ68727.1"
                     /translation="MTASVARGPIGRALDRLYSLAGYLAACFLFIIFLLMMALSLGRG
                     LRVNIPSGDDFAAWSMVAMAFLGLAHTFKNGEMIRVGLLLDRLDGKRRRVMEAATLTV
                     AVLFIGFFAFHAFRFAHDSWKLNDLSQGVVAVPLWIPQAFFFAGLAILLIAVIDELVH
                     VLRGNMPTFHKEPPKTTEELIERVVQGGGV"
     gene            13635..14939
                     /locus_tag="PK812_03895"
     CDS             13635..14939
                     /locus_tag="PK812_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRJ68728.1"
                     /translation="MSLVDLSLLLLLLLVCVLGSGVWIAIGLALVGLVAMLMTTSVPV
                     GTVLATTVWSATSSWTLAALPLFIWMGEILFRTRLSEEMFRGLAPWLQWLPGRLMHVN
                     VVGCGIFAAVSGSSAATCATIGKIALPELEKRGYDPGMSLGALAGSGTLGLLIPPSIP
                     MVVYAVTANVSVLQVFLAGFIPGALVMALYSGYIIIWSLLNPEKTPPRDPPLSFSEKL
                     RESRRLIPCLALILMVFLSLILGFATATECAAWGVTGALVLAWGSGTLTWQTFLDSLM
                     SATRLTCMIMLILAGAAFTTAAMAYTGIPAALAAWVDAMKLSPFALILALTLLYIILG
                     CLIDGISMIVLTAVIVLPMIKQAGFDLVWFGVYLVILVEMAQITPPVGFNLFVLQNMS
                     GKDSFTVAKAALPFFLLLNVAVAIITLFPQIVLYLPKLVFPD"
     gene            14977..15951
                     /locus_tag="PK812_03900"
     CDS             14977..15951
                     /locus_tag="PK812_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRJ68729.1"
                     /translation="MLRRTLLAGAALAAFALSGAAQAQTKWNLPAAYANTNFHTENLV
                     QFAKDVEAATGGKLAITVHGNASLFKAPEIKRAVATGQAQIGEVLISLHENEDAVFGV
                     DVVPFLATSFKDAKKLWDVSKAATEKKLASQGLMVLMAVPWPPQGLYAKQDINKVEDL
                     KGIKFRSYNAGTARIAELAGAQPVTIQASELPQALATGVVTSFISSGSTGYDSKVWES
                     LTHFYDTQAWLPKNITFVNKAAFDALPKDQQEALVKAAKTAEERGWKKAEELSGWYLK
                     ELAAKGMKVAPPSAELAAGLKKYGEDLTATWLKKAGADGQAVIDAFKK"
     gene            15956..16726
                     /locus_tag="PK812_03905"
     CDS             15956..16726
                     /locus_tag="PK812_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HRJ68730.1"
                     /translation="MTTPTGPEGGQPSGLSSSPARIGILALDTRFPRIPGDVGNPATW
                     PFPVRIETVRGATPERVVHHRAEGLFDAFAEHARALVADGVEAIVTTCGFLTLMQDNL
                     RRAAGAPVLTSALMQVPLVESMLAPGQRVGILTASARSLTPAHLLAAGAPADAPIAGV
                     PENGAFSATFLGNAPTLDEAVARREVVDAARRLMEQQARIGALVLECANMGPYAADVR
                     RETGLPTYDMVSLVTWLHLGLQPRVFPSCGHATLASPA"
     gene            16785..17522
                     /locus_tag="PK812_03910"
     CDS             16785..17522
                     /locus_tag="PK812_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HRJ68731.1"
                     /translation="MKKVYPDARTALADIARDNITVMAGGFGLCGMPEDLIIALRDTG
                     AKGITAISNNAGVDGKGLSLLLETRQIKKMISSYVGENKLFAAQYLAGELELEFNPQG
                     TLAERIRAGGAGIPAFFTKTGVGTIIADGKPVQEFDGEKYVMERWLRADISLVHAWMA
                     DTEGNLVFRKTARNFNPMMATAGKVTVVQCEHLVKPGDIPPDHIHTPGVFVQRIVHVP
                     TAAKYIENRTTRKRSSGAPAAGTGGEI"
     gene            17525..>18105
                     /locus_tag="PK812_03915"
     CDS             17525..>18105
                     /locus_tag="PK812_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015951344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HRJ68732.1"
                     /translation="MAWTREQMAARAAKELKDGFYVNLGIGIPTLVSNYIPDGMSVQL
                     QSENGMMGMGPFPFEGDEDPDLINAGKQTVSELPTTSYFSSADSFAMIRGGHIDLSIL
                     GAMQVAQNGDLANWMIPGKMVKGMGGAMDLVAGVKKVVVVMEHEAKGESKLLKACNLP
                     LTGANVVDMVITDLGVFSIDKKGKGGMVLIELA"
     assembly_gap    18106..18205
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(18220..18963)
                     /gene="truA"
                     /locus_tag="PK812_03920"
     CDS             complement(18220..18963)
                     /gene="truA"
                     /locus_tag="PK812_03920"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HRJ68733.1"
                     /translation="MPRYKLTIEYDGTAYVGWQLQAVGRSIQGALEQAVTRFSGEAAR
                     VNGCGRTDAGVHAIAQVAHVDLAKAWRTDVVRDAMNAHLKLMDDAVAVTAAELVPETF
                     DARFSARRRHYLYRIVNRRPPAPLEAFRAWHVTRRLDLDAMNAAAAVLIGKHDFTTFR
                     AANCQAKSPVKTLEALDCRANGETVEVRASARSFLHNQVRSMTGSLVEVGLGRWTVED
                     MRRALDARDHARCGPNAPPYGLYFVSVEY"
     gene            complement(19225..>19370)
                     /locus_tag="PK812_03925"
     CDS             complement(19225..>19370)
                     /locus_tag="PK812_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRJ68734.1"
                     /translation="GTVLDARLTVACGEGAVRLIEVQRSGKAPMQAADFLNGARIGPG
                     ATL"
BASE COUNT         3526 a         6360 c         6297 g         3087 t
ORIGIN      
        1 gcagggccgc gaccacgatg gaaccgagcg gcgcccgcgc caggcggaaa gccggcgcgc
       61 ccggcttcag cagcaggtgc gggggacggg cgccggatgc gtccggatgc agccgcgaga
      121 cacggttggc gccgcgtttc tcgacgctga gatgacgccg ccgggaggcg acccccagca
      181 tcagcacgca cgccggccgc acctctgcca ggatttcagg gatgtcccgc gtcacggcgg
      241 catattccgt aggaaagatg cgcacggccg tttcaatgcc ggctgccttc aaccgccgat
      301 ccgccgcgat gcgccgcgcc agcgcttccg atgggttttt cgggacgccc gggaacgggc
      361 cgaaagcggt gatgagcagg cgcatctaca accccagcat gacagccact tcatccgccg
      421 ccagagtggc ggaaagcgca ccgtcaaaga ccgagcgttc aatctcgggc agacgggcac
      481 ggaccttggc atcggtccgc aggcgcaggc gcagccggtc gtccagcatg gaccacatcc
      541 atttcacctg ctgccgcgcg cggcgcgcct ggaggtcgcc actgtcgtgg agcaacgcac
      601 gatgccggcg cacattaccc caaagggcgt cgagaccttc accgttgagg ccggaggtca
      661 ggacaaccgg cggcttccag ggtgcaccgt ccggggtcag caggttcaag gcggcggagt
      721 attccgtcgc ggtgatgcgg gcgcggggca gcgcatcgcc atccgccttg ttgatggcaa
      781 tcatgtcggc gagttcgatc acgcccttct tgatgccttg cagctcgtcg ccgccgccgg
      841 gaagcagcag caccagaaag aaatcggtca tgtcacagac cgctgtttcg gactgcccga
      901 tacctaccgt ctcgacgatc accacatcga agcccgcggc ctcacacagc agcatggcct
      961 cgcgcgtcct ggcggcgaca ccgcccagcg tcgcccccgc cggcgagggc cgaatgaagg
     1021 cattcggctc gaccgacagc cgggacatcc gtgtcttgtc gccaaggatc gcaccgcccg
     1081 tgcgcgacga cgtcggatcg accgccagaa cggccacctt tagcccctgc ccgatcagca
     1141 tcatgcccag cgcgtcgatg gtggtggact tgccgacgcc gggcactccg gtgagtccga
     1201 ggcggatcgc ccggccggtc agcggcagaa gctgttgcac caggttgcgc gccagcgcct
     1261 gatggtcgag ccgcttcgat tcgatcagcg tgatcgcgcg cgccaaagcc gcgcgatccc
     1321 ccgaccgcac acgcacggca agatcttcaa cggacggggc tgccggaatc gcggacatcg
     1381 gcgtgttata ggccgcgccg ccggcgcacg ccatgcgccg ggcgccgatg tgcacgccga
     1441 gcgccggggt catggtgaac aagcgctaaa cccggcatga acgatggctt caaacccggt
     1501 tcaacggcgg catcgtactt ttgaaccgtc gcctgaagga ggctccgagg gagccggaag
     1561 gcgacaggga gacgatccta tgaccatccg gttcgcgccg aaaatcctga ccgccgccgc
     1621 ggttctgggc gcggcccttt tcgccgcagc gccggcctcg gcgcagtatt acggctatca
     1681 tggcccgcgc gtggcggccc cgccgccgac ggtctatcac ggaccgcact acggggtgca
     1741 tccgcaccac cggccctgga agcgctgggc ctggcgtcat catcaccgcc cgccgccgcc
     1801 gtgggcctat ggcccgccgc gctacggcta ccagtggtag gaagcgcgtc gacgagtctt
     1861 ttcgagcttc cggcgcgcgg gggaaacccc gcgcgcgtgc attccagctt gatgacagcc
     1921 ggctgccgca ccaagatggc ctcgaatctg ccgaggtcac cgtgctcaag ctttacaatg
     1981 ccttcctcaa cacctgcgcc gggttcgcgc atggcctgcg cacggaacgg gcgatccaac
     2041 aggaagcgtt cctgctcgcc atcggcctgc ccctggcatt ctggatcggt gcgaccggtt
     2101 gggaacggct cgcgctggtg ctggcgctgg tcgcggtgct gtgcgtcgag ttcctcaaca
     2161 cggcgatcga gaagctgtgc gaccacgtca cgccggagcg ccatgatacc atcaaggcca
     2221 tcaaggacat gggatcggca gcctgcttct gcgcgcaggc gggttgcggg ctgatctggt
     2281 tgttcgcagc ctggcagcgc ttctggccct gaggaagcgg cgcgccggac gtcaggtccg
     2341 acgcgccgcc gggaatcaat gggcgtggcc gcccgccttc ccgcccagca ccgttggatc
     2401 aagcatgccg aacaccatgg cgatgtgcgc cacgacgagg aaaaccgcca cccacagggc
     2461 atgcagcccc atccgcgcat gctcatcctt gtccttcgcg acgagcttca gatcaatcag
     2521 cgcgatcatg cccagcggca cgactccggc gagataggcg aaaaccgcga tgtagtccgc
     2581 cggaccgcgc caggcaccgg cctgcgtgat gggcaggacc gccgtcgcca tgagatagac
     2641 gaacacgccg gcgaaataga tgccgccgac gatgctggcc aaccggttga gctggcgcgc
     2701 aacgccgtca taggcccgcg cgtacaggat gtagagctct gtgatggcga ccgtttcggc
     2761 caggatgacc ggcacggcca tgaagatgag aaggttccac ggttggttga ccgcgaggag
     2821 ctccatgtaa tgcgtcatgt tcattgaaat gacctccgat gggcgagctt gcgcatcgcc
     2881 gggattgcgt cgacttgcgt gtgggaatcc ggtgagctag cgacgcggag gacgttccgg
     2941 cggtccgccg ccggcctgtg gcaggccaat gtccgaagcc gggatcatcg gcgttgcgga
     3001 aacgaaagcc agcgcaagca tcgccgaaga cgtgatggcg acgggtgagg agatggcaca
     3061 gccgcaatcg caccgctgcg caccggcggg acaggtccgc acccgttcac cacacggcgc
     3121 gggcgacacc attgcgagcg gctgcaagac aggcgccttc gccgcatcgg gtccgggaac
     3181 cacagcacga tgatggtggt gaccatgacc gtgggcagcc gcggcgccgg catccggaag
     3241 ggccgatccg gcgataaccg cgaggacaag caggacaagg cgcagcatga gggttcgtcg
     3301 cattcctgaa catgagccct gatgcaggtt ctgcatgatc tggtctgcgt ccgtcaatcc
     3361 tgcgccggcg gctcctcggc cggttcctca atgaccggcg gtggctcggg cctggcgatt
     3421 gcattaccct tcgcatccag ccattcgccg ccaggcatcg gctgcggcac gccggtggtc
     3481 gtgggcagcg agagcggcag atcacgccgc gaccgcacag caaggtaggc gaagcactgc
     3541 gcctccagtg cgtcgccatc ccagccgaca gcttcgaccg gttcaaccgg gatgccgagg
     3601 cgcgcggcga aaagcttcat gaaggttgag ttgcgccggc caccgcccgt gaccagcaag
     3661 cgccctggcc tggaggcaag aaggggcagg catttcagcg tcgcctcgac ggtgaaggcg
     3721 gcgagcgtcg ccgcgccatc ggcgtccgag agtccctcga ccagggccgc gccctggtgg
     3781 aactcgttgc ggtccagcga cttgggcggc ggccggtcga agaagggatt gtcgagcagc
     3841 ttcatcaaca ccgcgacatt cacggaaccg gacgctgcca gcgcaccatt ctcgtcgaac
     3901 ggcttgccaa gccgcttctg cacgaaatca tcaagcagcg cgctcgcggg accggtgtcc
     3961 atggcgacga taccctcgcc atcgagccag gtgatgttgg caacgccgcc gagattgagg
     4021 atgatcagcg gcttttgcag cccggcggcc agcgcgcggt ggtacaacgg cacgaagggc
     4081 gcaccctcgc caccggccgc cacgtcggca tggcggaatt tccagaccac cggaatgccg
     4141 agccgccgcg ccagccgcgg accgtcgccg agctggcgcg tcagcttgtg ctgcggagcg
     4201 tggaagaccg tctgcccatg gaacccgaca aggtcgacct gctcgcgcgc gatggcgttc
     4261 tcggccatga accgcatgac cgcattggaa tgggcatcag cgactgcgtc atccagctcc
     4321 ggcatcggca gatgctcggc gcggtaagga ttgtcgataa ccgcgagcaa ctcttcgcgc
     4381 agcagcctgt gataggggta acttcgtgcc ggtcccgtgc gcacggcctg ctcgccgtcc
     4441 gtacgcacaa gggcaacgtc gatggcatcc atcgatgtgc ccgagatcac gccgattgca
     4501 gtttgcatga acgccgaatc aactttccga tcagggcgtc aaaatccggc gtttcaggac
     4561 tttgatcaag cgaaaagcct agacctttag tacgagcttg ccaaaattcc caccggagaa
     4621 cagcaggtcg acagtctcgg cgaatttcgc gaccccgcct tcgcgcacgt cttccttgag
     4681 cttcagcttg ccctcgtcgc gccagcgcac gagatcggcg acgccttccg ggtaacgctt
     4741 ggcccagtcg aacacgatca ggccctgcac gcgcaggcgc ttgaccagga tcacgcggaa
     4801 gttcttgacg ccttccggca tctcggtggc gttgtactcc gagatgaggc cgcagaccgc
     4861 gacgcgtccg aacaggttca tcatcggcag gatggtatcg agaatctcgc cgccgacgtt
     4921 ctcgaagttg acgtcgatgc cgttgggaca ggctgccttg agctgctggc gcaggttccc
     4981 ggccttgtag tcgacgcagg cgtcgaagcc gagttcctcc acgacatagc ggcacttctc
     5041 cggcccaccg gcgataccga cgacgcggca gcccttgatc ttggcgatct ggcccacgat
     5101 ggagccgacc gcacccgacg cggcggaaac gaccacggtc tcgccggcct tgggctggcc
     5161 gacgtcgagc aggccgaaat aggccgtcat gccgggcatg ccgaggccgc cgatccacgt
     5221 ttcgagaggc gcaagccgcg tatccacctt ggctacggcc ttgccatcgg acacggcgta
     5281 ttcctgaacg ccgaacaccc ccgtcacggc atcgccgacc ttgaaccgcg ggttgttgga
     5341 cgcttcgacg atgccggcgc cgccggcgcg catcacctcg ccgatctgca ccggcggcag
     5401 atacgacttt acgtcctgaa cccagccacg catggccgga tcgagcgaga tcaccttcac
     5461 acgcacgagg atttcgccct cgccgatgct cggaaccggc acttccttca cctcgaagtc
     5521 agacttccgg atgcggccca ccgggcggct ggccagaacg gccatgtggt tcgtcggcat
     5581 tgcaatcccc tcgaattgtt tagttctgaa ccaatctaag gacgacaagc cgacaagaca
     5641 aggtgatggc accatttccc gccttcaaat ctgccggagc ttggcctagg gaaggaaacg
     5701 tcactgccct tccaggatcg tccatgtcca agtttcccct tcccgatgtg catgttcagg
     5761 ccgaggtgat cagccaggcc ctgccgcaca tgcagcgcta cgaccagcag accgtggtca
     5821 tcaagtacgg cggccatgcc atgggcgaca aggcggccca ggaggatttc gccgaggaca
     5881 tcgtgctgct cgaacaggcc ggcgtgaagc ccatcgttgt gcacggcggc gggccgcaga
     5941 tcggcaggat gctggaaaag ctcggcatca agtccgagtt ccaggccggg ctgcgcgtca
     6001 ccgacaaggc gacggtggaa atcgtcgaga tggtgctggc cggggcgatc aacaagcaga
     6061 tcgtcggctg gatcggggcg gaaggcggca aggccattgg cctgtgcggc aaggacggct
     6121 ccatggtgac ggcgaagaag ctgacgcgaa ccatggtcga tcccgattcc agcatcgaga
     6181 aggtggtgga tctcggcttt gtcggcgacc cgcacaaggt cgaccgttcc gtcctcgacg
     6241 ccgtgctcaa atcggaactg atcccggtga tcgcgccggt cgcgatcggc gaggatggag
     6301 agacctacaa cgtcaatgcc gataccttcg ccggcgccat cgccggagcc atgcgcgcca
     6361 agcgcctgct gctgctcacg gacgtgccgg gcgttctcga caagaacaag cagctcatcc
     6421 cggaactgac cgtcaacgac tgtcgcaagc tcatcgccga cggcacggtg acgggaggca
     6481 tgatcccgaa gatcgaaacc tgcatctacg ccatcgagaa aggcgtcgag ggcgtggtga
     6541 tcatgaacgg gaccgtgccg catgccgtgc ttctggaact gctgaccgat cacggcgccg
     6601 gcacgctgat cacacggtga ggcggcgggc ccggactgaa tccgggctcg tccaatttcc
     6661 gattgccttg gtgccgagat gcgcgcctta tgcgctccgg tttcccggag cgctcatgct
     6721 gcagcctgcg acccgtccag cccccagttt cacgccttca ggaccgggcg tggtcaatgc
     6781 cgcggtctat tcgggcgggc ggcgcgtgcg ggacgtggcg atcgacgagg ccggcacctg
     6841 ggcacgcaag caagaccata tcgtctggat cggcctttac gagccggacg acgacctgct
     6901 caagcgggtc cagatgcaat tcggcctgca tgacctggcc atcgaggatg caagccacgc
     6961 gcaccaacgg ccgaaaatcg aggcctatgg agacgggcat ttcatcgtca cgcgcacggc
     7021 gcaaatcgtg gatgggcgcg cggcattcgg cgagacgcac atcttcgtcg gaaaaggcta
     7081 cgtcatatcg gtgcggcatg gagcctcgac cagctatgcg accgtgcgcg cgcgctgcga
     7141 ggcggcgccc aactcgctgt cgcacgggga ggactacatc ctctatgcga tcctcgattt
     7201 catcgtcgac aactacatgg acgtgctgga aaccatgcac gacgacgtcg aggcgatcga
     7261 ggacatcgtg ctgtccgaag cgctgactca ggaacagatc gagcggctct acaacttgcg
     7321 gcgcgacctg ctgcgcatgc gcaacgcggt gacgccgctg gtggacgtct gccggcggct
     7381 ggaagtgacg gattcggtct atatcagcga gaacatgcgc gtcttgttcc gcgacgtgac
     7441 cgaccacatc cgccgcgtgc aggaggaaat cgaatccttg cgcgaagtgt tggccttcgc
     7501 cttcgaaacc tcgctgatga ccggccaggc gcagcagacc gcgatcagcc ggaaactctc
     7561 ctccggcgcg gcgatcctcg cggtgcccac cgccatcgcc ggcatctacg gcatgaattt
     7621 caagcacatg cctgagctgg aaagccccta cggatactac gtcgtcatcg cggtcatcct
     7681 gacgctctgc tccatccttt tctggcgctt ccgcaagaac ggctggctgt agtccgttac
     7741 cccagcaggt ccgacttcga ggcttgcccg atatcccagt agagcccggc catgacgccg
     7801 agcgcctcgc gcaccagcgc cctgggaaca tgctcgtccg gcgcgtgctg cgagcagccg
     7861 ggataggagt ggggaaccca gaccgtcggc aggccgagca tgtcggagaa gatatcgttc
     7921 ggcaacgcgc cgccaagatt gggcagcacc gccggcgcct tgccggtggt ccgctcgagc
     7981 gaggcggcgg cgaagcgcac cgcgggatgg tccgggtcga gccgcgtcgc cgggaagtta
     8041 tcgtcgcgcg tcgtctccac ccgcaccatc gtcagcccct gacggtcgag atggcggcgg
     8101 atggcgggca cgatgcgcgc cggatcgatc ccgacgacat ggcgcaactg gcagcgcgcc
     8161 caggcgcggg gcggaatggc gttgaccggc gttctgggcg tgccggtctc gaaggcgagc
     8221 acctcgaaat tgcaccagcc gaacaccttt tccgccgcgc tgagaccagg ctcgccccaa
     8281 tccggctcga tcgccgggcc atccggaccg gagtcgatgg tgcaaccggc gagcgcgcgg
     8341 cgcaccgact ccgggatgcc gccttccgga accagttccg gcacgcggat ctgcccggtc
     8401 ggaccgacaa tcgtagcgat ggcgtgcgca agctggatgc cggggttcga gagcagcccg
     8461 ccccagttgc cggaatggtg cccgcccttg cgcgcctcga cgacaagatc gagctgatag
     8521 gcgccacgcg cgcccatgaa aaccgtcggt cgcccggcgg caaggcgcgg tccgtcggag
     8581 gcgatcagca ggtccgcgcg cagcaggtcg cggttctcgg aacaaacctc gcgcaggccc
     8641 ggcgaaccgg tctcctcgcc catttcgatg atccacttgg cgttgaagcc aagcttgccc
     8701 cgcgcggcga tcaccgcgcg ctgcgccgcg aggttgatgg cgtgctggcc cttgttgtcg
     8761 acaacgccgc gcccgtacca gcgatcaccg cgctcggtca gaacccaggg cgacaacccc
     8821 tccgaccacc cctcgtccag cccgcggatg acatcgccat ggccatagcc gagcacggtg
     8881 agcgcctgcg ggtcctcgat gcgctcggca tagagaaacg gccccttggc acgcggatgg
     8941 gccagcaccc gggtcgaaaa tccgatggcg gagaaggccg gcgccagctg ctgcgtcaga
     9001 tagtccgtca gcgcctgtgc ccgctccggg ttctggctct cggtgggaat ggcgatgcgg
     9061 cgggcgagtt catcccggaa tgcgccgcta tcgaattcgg cgtggacggc ggcaatggcg
     9121 gtttcgcggg acatgaccaa acctcgggtt ctttcaggca tgatggcagg cgacccgtcc
     9181 aggaccgccg cgcggcgaca ggtccgggcg ttcggcgcga caaatgccgg tcgccaatgg
     9241 gcagcggctg tgaaacgcgc aacccgacgg aacattggac gggcttggcg gctcgccggc
     9301 aagcggcagg cgcgcacggg cgcgctgcac gacgggatca ggctcgggca ccgcttgcat
     9361 cagcgcccgc gtatagggat ggcgggggtt tgtgaacagg gcatcgcgcg gcgcctcttc
     9421 gacgatgcgt ccgagataca tcacggcgac acggtcgcag acgtgatgca ccacggcgag
     9481 gtcgtgtgac acgaaaaggt atgacaaccc gcgctcgcgc tgcaaccggg caagcaggtt
     9541 cagaacctgc gcgcgaaccg acacgtccag ggccgagacc gcctcgtcgc agatcagcag
     9601 gtcgggcgaa agcgcgaggg cacgggcgat gccgatgcgc tggcgctgcc caccggaaaa
     9661 ctgatgcggg aatcgcgcgg catgttccgg gttgagcccg accgctcgca acaggtccgc
     9721 gatgcgccgg gccagttcct cgcccttcgc caggccgcgc accagcaggg gttcgccgat
     9781 tgccgcaccc accgacaggc gcggattgag ggacaacgcc ggatcctgga aaaccatctg
     9841 tgccctggcg cggatggcgc gcagcatctc gcgatccgcg ccgacgacct cctggccatc
     9901 gatgcggacg ctgccggctg tcgcccggac aagacccaag gcggcgttcg ccagcgtgga
     9961 ttttccgcag ccggattccc ccacaagtcc cagcgtctcg cccctggcga cggtcaggga
    10021 aaccccgtcc accgcgcgca ccggcggcaa acgcggcccg aaccagcgct gccgggcggc
    10081 aaaatgcacc tggagcgcgt caatggcgag gacaggcacc atcatggcgc cattcccgga
    10141 gcgagtgtcg cggaaaaggc cgcgacatcg gctgtgcgcc agcaggcgac agccccctgc
    10201 ccttcgggcg tcaacggcgg ggcctcgcgc cggcagcggt cgcgaaccag cggacagcgc
    10261 gttgaaaagc ggcacccagc cggcatgtcc tgcgccgtcg gtacagcgcc ctcgatcgcc
    10321 ggcaggtcag tcttcgacgg acgcgacagg cgcggaatgc tggcgaggag caaggccgaa
    10381 tagggatgcg ccggggcggc aaagatccgc gcggccggcc cggtctccac gatgcgcccg
    10441 gcatacatca ccgcgacgcg atccgccatg tcggccacca cacccatgtc gtgggtgatc
    10501 agcaggatgg aggcgccgcg gtccaccacc agcgtcttca tcaggtcgag gatctgcgcc
    10561 tgaatggtca cgtcgagcgc agtcgtcggt tcatccgcga tcagcaggcg cgggcggcag
    10621 atcagcgcaa tggcgatcat cacgcgctgg cacatgccgc ccgacagttc gaacgggtac
    10681 tggcgcagtc tcctgtccgc atcggcgata ccgacggcat tcagcatcga acgggcccgt
    10741 tccagcgcct gcgccttgcc gacagcttca tgcgccatga ccgcctcggc gatctgggcg
    10801 ccgaccgtgg tgagcggatc gagcgaggcc gccggctcct ggaagatgat cgacagctgc
    10861 cgcccgcgca ggtcccgcat ccgcgtctcg gacaggcgca gcacgtcctg accgcccacg
    10921 gcaatgccgc cgctgacggc ctcgaccgcc ggtggcagca agcgcatggc cgccagcgcc
    10981 gtgagcgact tgccgcaacc ggattcgccc acaagggcca ccacttcccg ttcgccgact
    11041 tcaaggctca ggcgatccac aaccgcatgt ccggcgatgc gcaaggtgag gtcgtggatg
    11101 gacagaaccg gcgcggccat ggctcagcgt ccctgcgccc gcgggtcgag atggtcgcgc
    11161 agcgcgtcgc cgagcacatt caccgacagg accagcatga acaggcaggc gccggggaag
    11221 gccgtcatcc accaggcccg ctccagatag ttccggccga ccgacaggat cgagccccaa
    11281 ctcggatcgg gcggctggat gccgaggccg aggaaagaca gcccggcctc gaacaggatc
    11341 atcaggccaa actccgcagt caccacgacc agcaacggcc ccgcgatgtt gggcaggatg
    11401 tggcgcagaa gcaggcgcgg cgccctcacg cccgccagtt ccgcggcttt cacatacggc
    11461 agcgtcgcgc tctgcaacgt ctgcccgtag gcgacgcggg cgtagcgcgg ccaccgcgtc
    11521 aacccgagca cgatcacgag gttgagcaat cccggcccca ggaccgcgac ggagagcacc
    11581 gccagcagga tcgcgggaat ggacatcatg atgtcgacga gccgcatcag cacgatctcc
    11641 gtcacgccgc gaaaccaggc ggcggccatc cccaccgccg tgccgacgac gcaggacagc
    11701 acgaccgaga ggacagcgat ggcgatcgac agacgcgcgc cgtgcagaag gcgcgcatag
    11761 agatctcggc cgagatcgtc ggtgccgagc cagtaggtca cgccggcggc ggtcgtgaag
    11821 cccggcggcc gcaaccgcgc cagaagggtc tgacgcgcag gatccagcgg cgagagccag
    11881 ggcgcggcaa gcgcagccag caggaacagc gccaggacga gcgccgcgat ccgcgccgcc
    11941 aagggcagcc ggttcaggac ggcgcgcccc tctctcagcg ccacaatcat gatcgcgcca
    12001 gcacgatgcg cgggtcgatc caggcataga cgaggtccgc cgccagactg gttgcgacat
    12061 aggtcacgga gatgaacagc acgatcgcct gcacaagcag gaagtcgcgg ctctggatgg
    12121 cctgaagcgc gagttgcccg acgccgggcc aggcaaaaac gttctcgaca atgatcgagc
    12181 cggccaggag attgccgatc tccagcgcgg cgacggaaac gagcggcagg gcggcattgc
    12241 gcaggacgtg ccggaagacc gccgtccaga tcgaaagccc ccgcgcgcgg caggcgcgca
    12301 catagtcctt cgacagttcg tccaggaccg cgatgcgcgc catgcgggcg aaggtcgcca
    12361 tggacagggc gccgagcgcg atggccggca tgacgatgcc cgaggccgaa tccgcccccg
    12421 acgagggcag ccagcccagc gtcaccgcaa aaaccatgat catcaaaatg ccggacagga
    12481 aggtcggcat gctctgtccc gccagagtga gcgcgccgag cagcctggcc agccaatggt
    12541 caggccgtac cgccatgatc acgccgacgg gcaccccgat gccgatggcg acagtcaagg
    12601 cagcgagcgc caggctcacg gtgtagggaa ggcgcagcat caggatgtcg atggccggcg
    12661 cgcgctgcac gaccgattcg cccaggttga ggcgcagcag gtcgccgaca taggcgaagt
    12721 actgcacatg aagcggccgg tcgaacccga gcgccacgcg caaggcctcg atctgctcgc
    12781 ggctggcgtt gtccggcgcc agaagcaggg tcggatcgcc ggcgaggcgc atgaccacga
    12841 aaacaatggt caggacgcca agcgtggcga tcacggcctg cagcaggcgg tacaggatgt
    12901 agcgcgtcat ggctcccccg cgacggcccg gccgccgcgc cggcaggaag gcgagcgcag
    12961 ccgtcggagt caagccatgg gcatcggcaa ttcggctctg gccaaatcgt ggcgcaccgg
    13021 tctagtctgc cgaaaagccc gggaggggag cggggtcgat atgacagcat cagtggcgcg
    13081 ggggccgatc ggcagggcgc tggatcgact ctacagtctt gcgggctatc tcgccgcctg
    13141 ttttctgttc atcatcttcc ttctgatgat ggcgctttcg cttgggcgag gacttcgcgt
    13201 caacattccc tcgggcgatg atttcgccgc ctggagcatg gtggcgatgg ccttcctcgg
    13261 cctcgcgcat acgttcaaga acggcgaaat gatccgcgtc ggcctgcttc tggaccgcct
    13321 cgacggcaaa cgccgacgcg tgatggaagc ggcgaccctc accgtcgccg tgctgttcat
    13381 tggcttcttc gcgtttcacg cgttccggtt tgcccacgat tcctggaagc ttaacgacct
    13441 gtcacagggc gttgtggccg tacccttgtg gattccgcag gcatttttct tcgccggtct
    13501 tgcgatcctg ctgatcgcgg tcatcgacga actcgtccac gtgctgcgcg gcaacatgcc
    13561 gactttccac aaggagccgc caaagaccac ggaagagctg atcgaacgcg tggtccaggg
    13621 cggaggcgtc tgaggtgagt ctcgtcgatc tgtcgctgtt gttgctgctg ctccttgtct
    13681 gtgtgctggg aagcggcgtc tggatcgcca tcggcctggc gctggtcggc ttggtggcga
    13741 tgctgatgac gaccagcgtg cccgtgggca ccgtgctggc gaccacggtc tggagcgcca
    13801 catcctcgtg gacgctggca gctctgcctc tgttcatctg gatgggtgaa atcctgtttc
    13861 gcacccggtt gtcggaggag atgttccgcg gtcttgcccc gtggctgcaa tggcttccgg
    13921 ggcgactgat gcatgtcaac gtcgttggct gcggtatctt cgccgccgtg tccggctctt
    13981 ccgccgcaac ctgcgccaca atcggcaaga tcgccctgcc cgaactcgaa aagcgcggct
    14041 acgatcccgg catgagcctt ggcgcgcttg ccgggtccgg cacgctcggc ctgctcattc
    14101 cgccatcgat cccgatggtg gtctatgcgg ttaccgccaa cgtctccgtg ttgcaggtgt
    14161 ttctcgccgg attcatcccg ggcgcattgg tgatggcgct ctattcgggc tacatcatca
    14221 tctggtcgct tctgaacccg gaaaagacgc cgccgcgcga tccgccgctg tcattttcgg
    14281 aaaagctgcg ggaatcgcgc cggctgatac cgtgtctggc gctcatactg atggtgttcc
    14341 tgtcgctgat cctcggtttc gcgacggcga cagagtgcgc ggcctggggc gtgaccggcg
    14401 cgctggtgct ggcctggggc agcgggacgc tgacctggca gacattcctc gacagcctga
    14461 tgagcgcaac gcggctgacc tgcatgatca tgctgatcct cgcgggcgcg gccttcacca
    14521 cggccgccat ggcctatacc gggataccgg cggcgctcgc ggcctgggtc gacgcgatga
    14581 agctctcgcc gttcgcgttg atcctcgcgc tgaccctcct atacatcatc cttgggtgcc
    14641 tgatcgacgg catttcgatg atcgtgctta cggcggtcat cgtgttgccg atgatcaagc
    14701 aggcgggatt cgaccttgtg tggttcggtg tctatctcgt catcctggtc gagatggcgc
    14761 agatcacgcc gccggtcggg ttcaacctgt tcgtgttgca gaacatgagc ggcaaggaca
    14821 gcttcacggt ggcgaaggct gctttgccgt tcttcttgct gctgaacgtt gcggttgcca
    14881 tcatcacgct gttcccgcag atcgtgctct acttgcccaa actcgtgttt ccggattgac
    14941 ttgaaccaac cagcattttc atcagggaga acaccgatgc tgagacgtac ccttctggcc
    15001 ggcgctgcgc tggcggcttt cgcgctgtcg ggagcagccc aggcccagac caagtggaac
    15061 ctgccggcgg cctatgccaa caccaatttc cataccgaaa atctggtgca gttcgccaag
    15121 gacgtggaag ccgcgaccgg cggcaagctc gcgatcacgg tccacggcaa cgcctcgctg
    15181 ttcaaggcgc cggagatcaa gcgcgccgtt gcgaccggcc aggcacagat cggagaggtg
    15241 ctgatttcgc tgcatgagaa cgaggatgcc gtattcggcg tcgacgtcgt gccgttcctc
    15301 gccacctcct tcaaggacgc gaagaagctc tgggatgtgt cgaaagccgc gaccgaaaag
    15361 aagcttgcca gccagggcct catggttctg atggcggtgc cttggccgcc gcagggtctt
    15421 tacgccaagc aggacatcaa caaggtcgag gatctgaagg gcatcaagtt ccgctcctac
    15481 aatgcgggaa ccgcccgaat tgctgaactg gctggcgcgc agccggtgac catccaggcg
    15541 tcggaactgc cacaggcgct ggccaccggc gtcgtcacct ccttcatctc ctccggctca
    15601 accggctacg attcgaaggt ctgggaaagc ctgacgcatt tctacgacac gcaggcctgg
    15661 cttccgaaga acatcacctt cgtcaacaag gcggccttcg acgccctgcc gaaggaccag
    15721 caggaagctc tggtcaaggc cgcgaaaacc gccgaggaac gcggctggaa gaaggccgag
    15781 gaactgtcgg gctggtatct gaaggaactc gcggccaagg gcatgaaggt cgccccgccg
    15841 agcgcagagc ttgccgccgg cctgaagaaa tacggcgagg acttgaccgc gacctggctg
    15901 aagaaggcgg gcgcagacgg ccaggccgtg atcgacgcct tcaagaagtg aagccttgac
    15961 gacgccaaca ggcccggagg gcggtcagcc gtccgggctt tcttcatcgc ctgcccgcat
    16021 cggcatcctg gcgctggaca cgcgttttcc gcgcatcccc ggcgatgtcg gcaatcccgc
    16081 cacctggccg ttcccggtcc ggatcgaaac cgtgcgtggt gcgacgccgg agcgcgtcgt
    16141 gcaccatcgt gcggaagggc tgttcgatgc ctttgcggaa cacgcgcgcg ccctggtggc
    16201 ggatggtgtc gaagccatcg tcaccacctg tggatttctt accctgatgc aggacaacct
    16261 gcgccgggct gccggcgcgc cggttctcac ctccgcgctg atgcaggttc cgctggttga
    16321 gtcgatgctt gctcccggcc agcgcgtcgg catcctaacg gcctcggcgc gttcgctgac
    16381 gcctgcgcac ctgcttgcgg cgggtgctcc agcggatgcg cccatcgccg gcgtgccgga
    16441 aaacggcgca ttctctgcaa cgttcctcgg caatgccccg acgctcgacg aggcggtggc
    16501 gcggcgtgag gttgtcgacg ccgcgcgccg actgatggag caacaggcca ggatcggcgc
    16561 gctcgtgctc gaatgcgcca acatgggtcc gtatgccgcc gatgtgcgcc gggaaacggg
    16621 cctgccgacc tatgacatgg tgtcgctggt cacctggttg caccttggcc tgcagccccg
    16681 ggtttttccc tcctgtggtc atgcaacctt ggcatcgccc gcctagccaa ccatgttgcg
    16741 ctgcggtata gccgcgccgt gatcacacaa ggagcagcgg cttcatgaag aaggtctatc
    16801 ccgacgccag gacggcgctg gcggatatcg ccagagacaa catcacggtc atggccggcg
    16861 gtttcggcct gtgcggcatg ccggaggatc tcatcatcgc gctgcgtgat accggcgcga
    16921 agggcatcac ggcgatctcg aacaacgctg gcgtcgacgg caagggcctc agcctgctgc
    16981 tcgaaacgcg ccagatcaag aagatgatct cgtcctatgt cggcgagaac aagctgttcg
    17041 ccgcgcagta cctcgccggc gagctggagt tggagttcaa cccgcaggga accctggccg
    17101 agcgcatccg cgccggcggc gccggcatcc cggcgttttt caccaagacc ggggtcggca
    17161 ccatcatcgc cgacgggaag ccggtgcagg aattcgacgg cgaaaagtac gtgatggagc
    17221 ggtggctgcg ggcagacatt tcgctcgtcc acgcctggat ggcggacacc gagggcaacc
    17281 tggttttccg caagacggcg cgcaacttca acccgatgat ggcaaccgcc ggcaaggtga
    17341 ccgtcgtgca gtgcgagcat ctggtgaagc ctggcgacat tccgccggac cacatccaca
    17401 cgccgggcgt gttcgtgcag cgcatcgtgc acgtgccgac cgccgcgaaa tacatcgaga
    17461 accgcacgac ccggaagcgg tcgtccggcg cgccggccgc cggcaccgga ggggagatct
    17521 gacgatggcc tggacccgcg aacagatggc ggcgcgcgcc gccaaggagc tgaaggacgg
    17581 cttctacgtc aacctcggca tcggcatccc gacgctcgta tccaactaca tcccggacgg
    17641 catgagcgtg cagctccagt ccgagaacgg catgatgggc atgggcccgt tcccgttcga
    17701 gggcgacgag gacccggacc tgatcaacgc cggcaagcag acggtctcgg aactgccgac
    17761 gaccagctac ttttcctcag ctgactcctt cgcgatgatc cgcggcgggc acatcgacct
    17821 gtccatcctc ggcgccatgc aggtggccca gaacggcgac ctcgccaact ggatgatccc
    17881 gggcaagatg gtgaaaggca tgggcggcgc gatggatctc gtcgccggcg tgaagaaggt
    17941 cgtcgtggtc atggagcacg aggccaaggg cgaatccaag ctgctcaagg cctgcaacct
    18001 gccgctgacc ggcgcgaatg tcgtcgacat ggtcatcacc gacctcggcg tcttctcgat
    18061 cgacaagaag ggcaagggcg gcatggtcct gatcgagctc gccgannnnn nnnnnnnnnn
    18121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18181 nnnnnnnnnn nnnnnnnnnn nnnnnggcgg ccgccttttt caatactcca cgctgacgaa
    18241 atacaatccg tagggcggcg cgtttgggcc gcagcgggcg tggtcgcgcg catccagagc
    18301 gcggcgcatg tcctccaccg tccagcggcc gaggcccacc tccaccagcg atcccgtcat
    18361 cgaacgcacc tgattgtgca gaaacgagcg ggcggacgcg cgcacctcga cggtctcgcc
    18421 attcgcgcga cagtccagtg cctccagcgt cttcaccggg cttttcgcct ggcagttggc
    18481 ggcgcggaag gtggtgaaat cgtgtttgcc aatgaggacg gctgcggcgg cgttcatcgc
    18541 atcgagatcg agccgccgcg tcacatgcca ggcgcggaag gcttccagcg gcgcgggcgg
    18601 acgacggttg acgatgcggt agagataatg ccgccggcgc gcggaaaacc gggcatcaaa
    18661 cgtctcgggc accagttccg ccgccgtcac cgccacggca tcgtccataa gcttgagatg
    18721 ggcgttcatg gcgtcgcgca ccacatccgt ccgccaggcc ttcgccagat cgacatgcgc
    18781 gacctgcgcg atggcatgca cgccggcgtc ggtgcggccg cagccgttga cgcgggcggc
    18841 ctcgccggaa aaccgcgtga ccgcctgttc cagagcgccc tgtatggagc gccccacggc
    18901 ctgtagctgc cagcccacat aggccgtgcc gtcatattcg atggtcagct tgtagcgggg
    18961 cattatactg tctgatcacg aacggggagg ctgcaaatgg ccctggtgag agcatttgtc
    19021 gagaagaaca tggatcgaaa ctcgatccat gatgggatcg atgcgacatt cacgatcttt
    19081 gaacgcgatg gccgctcatt tctgcaaatt gacacatatg ggagaaactc cagagatatt
    19141 cctggaaaga aaagccaaac aattcagttg gatagagagg gcggtcaagc actcttcaac
    19201 attttgaagc gcgcatttca tttatcataa agttgcgccc ggtccgatac gcgcgccgtt
    19261 gaggaagtcg gcggcctgca tcggcgcctt gccggagcgc tgcacctcga tcagccgcac
    19321 cgcgccctcc ccgcaggcga ccgtgaggcg cgcgtcgagc acggtgcccg
//
LOCUS       DAOMXN010000025        18458 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_1735_length_18458_cov_12.218334, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000025 DAOMXN010000000
VERSION     DAOMXN010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 18458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18458
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1735_length_18458_cov_12.218334"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..1018
                     /locus_tag="PK812_03930"
     CDS             134..1018
                     /locus_tag="PK812_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HRJ68735.1"
                     /translation="MSIRSMTGFARAQGTGAGFSWTFEAKSVNGRGLDARFRLPAGFD
                     MLEPDMRSRTQKALTRGNVNVTLTVRRDASSTGVRINETLFAHLAEAAERLAWTAKLP
                     SMTAGDILRLPGVIESGDAAEEGPPESVIAAVLTSFDAVLADLVLSRRSEGAALERII
                     AGQIDGIETGIGTAEAADSRRSDAIRSRLETQIAALLGTADRFDPDRLHQEAMLIAAR
                     IDVKEEIDRLKAHVAAARALLASGEAVGRKLDFLAQEFVREANTLCSKANDIALTRAG
                     LDLKTWVEQFREQVQNIE"
     gene            1023..1697
                     /gene="gmk"
                     /locus_tag="PK812_03935"
     CDS             1023..1697
                     /gene="gmk"
                     /locus_tag="PK812_03935"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HRJ68736.1"
                     /translation="MTNILASRQITDRRGVMLVISSPSGAGKSSLSRLLLQEEKERIS
                     LSVSVTTRKRRASEIDGVHYHFRSVPEFEVMRERGELLEWAQIHDNFYGTPRGPVEKA
                     LADGHDVLFDIDVQGTLQLYEKMREDMATVFILPPSIPELHNRLKRRADDDDQVILRR
                     LKTALSEIRHWTDYDYVLVNDDLDRCFFELQSVLAAERLKRSRRPALAGLVKTLDKDL
                     TALLGA"
     gene            complement(1704..2546)
                     /gene="rsmA"
                     /locus_tag="PK812_03940"
     CDS             complement(1704..2546)
                     /gene="rsmA"
                     /locus_tag="PK812_03940"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HRJ68737.1"
                     /translation="MAQIDDLPPLREVIREHGLVARKALGQNFLLDLNLTSRIARAAG
                     PLEGHVVLEVGPGPGGLTRALLAAGADRVIAIERDERCLPALAEIAARYPDRLDVISG
                     DAMAISPDRLIAGRTTRIVANLPYNVGTALLIGWLRAPTWPPFWKSLTLMFQKEVAER
                     IVATPQTPESYGRLGVLAGWRARSRILFDVAPSAFTPPPKVTSSVVELVPNPAPLPCQ
                     VQALERVTAAAFGQRRKMLRQSLKPLGDAAALLERAGIDGTRRAETLSVAEFVTIANA
                     VACP"
     gene            complement(2559..3473)
                     /locus_tag="PK812_03945"
     CDS             complement(2559..3473)
                     /locus_tag="PK812_03945"
                     /inference="COORDINATES: protein motif:HMM:NF020872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRJ68738.1"
                     /translation="MRYVIGVLLALAIQAATYSAITPAAAQTVLAFVNGEPITSVDVE
                     HRIRIMQVLARTGLTQKQALEQLIDDRLKIIEARRIGYRLKDDNIEDRMSSIAMQNNQ
                     TLPEFIANLSRAGVDTQAYKLKLRADYSWELALSQKGKAAAAVGADSEVREVYQKKLN
                     EGAAKVTDYVIVTVIFVVPRTGEGVGQREREAQAARGAFSSCETGIDDLRKLRDVAIR
                     APVKRSSNQIPPQLNKIFADTPVGRMSPPMRTTQGIELYAVCEKIERIDQSAVRTTAE
                     QEVASKRNATLAEGYLKNLRKTAVIQYR"
     gene            complement(3485..6073)
                     /locus_tag="PK812_03950"
     CDS             complement(3485..6073)
                     /locus_tag="PK812_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HRJ68739.1"
                     /translation="MMIRPGLRLCSLTVLALLVAGAASVAPGPAWAQKAKSAAPAPKP
                     TDKLMVEANELVYDKEKDTVSAVGNAQLYYQGRTLEADRVIYDRKSKRVFAEGNARII
                     EANGTKYFGDRFELTDDFKNGFIDSLRSQSPDKQRFSAARAERTEGETSVFERGTYTP
                     CEPCKTDPKRPPLWQIRAARIIHKTSEQAIYYEQATLEFWGVPIAYMPFFSGPDPSVN
                     RKTGFLMGRITNKKSLGTGVSAPFFWALAPNMDVLLTPTYYSRQGFLGQAEFRHRLQT
                     GTYTLRIAGINQNERDAFLPGPLGAGDRKFRGSVETTGQFYINEKWKWGWDLAAATDR
                     FFFRNYSIKTDAFRNTYQGEFVSQVYLNGQGERSWFDLRAQHFTPATNAEWLKNQAHA
                     HPVLDYDRRFSSSFIGGEVQLTANLTSLSRSAAHFQALPTPNSVGHAGSPNIFSGTAY
                     RQNGAAFGYGLYEGCNWYTRTNCILRGMSGNSTRATVALSWRRSFIDPVGQVWTPFAS
                     VRGDVLSYNLDTTRYVNAPLDNAIWGNDKLTNFVRDSSDVVGRVMPTVGVTYRFPFIA
                     FAGNITHQIEPIAQIIARPNERRATYMPNEDAQSLVFDDTVLFSTNKFSGYDRTEGGV
                     RANYGFQYTMTAASGASMNVLVGQSNQLGGRNSYSTPDASNTGLNSGLEKHRSDFVGR
                     IAVSPVANLNFSARGRFDEDTFKLRRLEIGASASTGPVSLSTSYARLAPQPMLGYNLP
                     REGWSSTLSLRLPSNWYASSSITFDMDRRHQDRSYHVAYPDLYPRYNSSPFRVSGIST
                     TLGYRDECIDFGVSWARQTSDMVTSGTNKIGTVYMFRLELKHLGQVQYRGTSGISTQN
                     DSSSGQ"
     gene            complement(6070..7161)
                     /gene="lptG"
                     /locus_tag="PK812_03955"
     CDS             complement(6070..7161)
                     /gene="lptG"
                     /locus_tag="PK812_03955"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HRJ68740.1"
                     /translation="MTFLVGTLGRYFALRFAKAILGVFLTVFILIFTLDLVELMRRSG
                     EAPNATTLALTRLALLRTPATAEQILPFAALFGALATFLNLSRRLELVVARAAGVSAW
                     QFSLPGIIVAALIGLLATVAYNPLATHFRARAEAYEASLFSRGGVGGNEKGVWLRQRS
                     VDGETILRAARLDENTFSLVDVTAFSFDPNGKFMERIEGQRAIHLGNHWRFENVRVLT
                     VDNEPQQMERYLLATSVSREQLRGSVGQSQAVSFWALPSVVRGLELAGLDATRYRLRF
                     QSLLARPALLTAMVLVAASVSLRFLRSGGFAGMVLSGVSAGFVLYVATQIVEDLGSAG
                     LVSTVFAAWAAPVIGGMLGSLALLHREDG"
     gene            complement(7158..8384)
                     /gene="lptF"
                     /locus_tag="PK812_03960"
     CDS             complement(7158..8384)
                     /gene="lptF"
                     /locus_tag="PK812_03960"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HRJ68741.1"
                     /translation="MQILERYIFRTAVSAFLLCLCALTGVIWISSALKELDLITGKGQ
                     SVLLFLYITLLSIPALIMIIAPLALFISIVYTLNKFNGDSELIVMSAAGVPPMKILRP
                     FLTLTLATAMLVGLITIKVMPESFRSLRDLVVKMRADLITQFVQEGKFVTLDAGITFH
                     YKEKGPNGILLGIMFQDRRNANMVTTYLAERGRMVEVDGLQYFLLEKGSLQRQDKARK
                     ESALVYFERYAFDMDQFGGENEKIVYKPRERTTWELMTLDKEEFYVKWQIGRFRAELH
                     ERFVQPLYAFAMLAIAFAALGSARTTRQGRGAAMAAAGIAVLLLRIAGFWASSLGARG
                     SFGIAMMYVVPIAAVGMAATYSWWTQDRPQVLVRAVDGLTAVSNRLGLTRQLRAINPG
                     AIVTQMAQLSMKLRRS"
     gene            8647..10137
                     /locus_tag="PK812_03965"
     CDS             8647..10137
                     /locus_tag="PK812_03965"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003610626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HRJ68742.1"
                     /translation="MSRGPRLSFASPDSGLHPVVVLFVGDDLALPKAAGRLGPGFPAL
                     FKTACDGEGYKGKAGSTLDLVAPAGVPANRVIVVGTGGDGDRAKGDWIGIGGKVMGKV
                     PAGRAVSVLFDAPGADAPAAADFALGIGLRAYRFDRYKTKKKPDEENGAAGDVSVHLT
                     DFAGAKKAVKSRDAVLDGVNIARDLVNEPPNVLTPKEFAARAAQLKKVGVDVEILGEK
                     EMRKLGMGALLGVGQGSVQESQLAIMRWNGTKDSKAAPVAFIGKGVVFDTGGISIKPA
                     AGMEDMKGDMAGAACVVGLMHALAARKAKVNALGAIGLVENMPDGAAQRPGDIVTSMS
                     GQTIEIINTDAEGRLVLADVLWYVQDRFKPKFMVNLATLTGAIMVALGKEYAGLFSNN
                     DDLAGRLFEAGGKTGEKVWRMPLGPEYDKIIDSKFADMKNSGGRFGGSITAAQFLQRF
                     VNSVPWAHLDIAGTGMDAASTDINRSWGNGWGVRLLDRLVADHYEG"
     gene            10160..10444
                     /locus_tag="PK812_03970"
     CDS             10160..10444
                     /locus_tag="PK812_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014889576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRJ68743.1"
                     /translation="MLAACIGAVHAQQPRPHRGVDIAADCAPCHGVDGIAKERDVPHL
                     AGQNAGYLYNQMKAFQSGKRPHKEMRFMSRPLTEAEMQAIAEFYATLPPR"
     gene            10641..11834
                     /locus_tag="PK812_03975"
     CDS             10641..11834
                     /locus_tag="PK812_03975"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRJ68744.1"
                     /translation="MLTTVLNKSAGSDLTRMSGSQHAGFTVAVRRDVGHAVAVAARLL
                     QGGSATVFQSAVWVEAFARTVAAERGCAFFMVEVRATESGRPVMLLPFLLRERRGFRL
                     LEVPDFGLADYVAPMFARGFDPDRQTMHRIWAQVRAVLPKADVVRFVKMPGEVGGRVN
                     PLVRLSGTRCSSLTAWGVDLGPVETIWRDAIACDKRRADLDARWRKLNKRGVVEFRTA
                     STEADADAFFETMVQQRSERFRALNFPNALDKPEIRDLYRSLLRPGEPASPAVVQALL
                     VDGRIVATGYGLVGNGAFHMIFPTFQAETWRNYSPGLQLFRKSMEWSARKGLRYYDFT
                     VGSEGFKRDFGAREQPLFERYEALSFAGLLPVGYVALHRFIRTQPWLASLLRAVKSMF
                     MSGGR"
     gene            11836..13047
                     /locus_tag="PK812_03980"
     CDS             11836..13047
                     /locus_tag="PK812_03980"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRJ68745.1"
                     /translation="MSTFVADSTAVAPHRYAVLDAWRGICALLVTVVHIPLAHRWLGA
                     QWFENMQMFVDFFFVLSGFVMCHAYGHKLDEPVRYNSFMIRRFGRIWPLHAAILAGFV
                     LLELVKLGAQVGLKLPMDGEAFTGPRSVATLVSNVFLTQSFNLHGMTSWNGPAWSIGV
                     EFYAYMVFGLVVFYAGMRTAAFAALATVGLAGVGLFAPNWIFTTHDYGFFRCLFGFFT
                     GCLVYQAVQKGRPAWSGVTAEVAAVIVLVAFMLLTGRNATSLAAPIVFAGVVYVFAFE
                     RGPLSAALRTAPAQALGLWSYSIYMVHMLIFAVEKIALSVGEKVAALKLTSVVTPTGK
                     FWTFNNGVLDTGFFAMQIALTLLASAITYRLIEEPARRWFARLADAYERKHAPSPQGG
                     LLATRMAGLVR"
     gene            13129..13578
                     /locus_tag="PK812_03985"
     CDS             13129..13578
                     /locus_tag="PK812_03985"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003602126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HRJ68746.1"
                     /translation="MVEVWFYHLTRGSLEDTLPALVERTRARGWKAVIRVGTAERMAA
                     LDDLLWTYDDESFLAHGTASEPDAQVQPVLLTTADGLPNDADVLFLADSAPLPGEWPR
                     ERVIMLFDGADPDALEHARAAWKEAITLVHPVQYWQQDDSGRWSRKA"
     gene            complement(13652..14941)
                     /locus_tag="PK812_03990"
     CDS             complement(13652..14941)
                     /locus_tag="PK812_03990"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HRJ68747.1"
                     /translation="MRFLCRDCLTDTSSNDHVARCPGCGSPRVLTHPERDRLAIAHVD
                     CDAFYAAVEKRENPTLADKPVIVGGGKRGVVSTCCYIARTFGVRSAMPMFKALKACPE
                     AVVIKPRMELYSAVGREIKARMLELTPLVESVSIDEAFLDLSGTERLHGASPAVTLAR
                     FARRIESDMRLTVSVGLAQNKFLAKIASDQEKPRGFTIIGTDEALDFLAPRPVGLIWG
                     VGHAMQDRLARDGIRVIADLRRVGEADLYRRYGSEGGRLFRLSRGIDSRRVTPSHPIK
                     SVSNETTFNDDLSTEVDLAPILWRLCEKVAARLRHSGVCGASITVKLKTADFRLVTRA
                     RSGLPPTALAVRIHDVARDLLRHELTGVRYRLLGVGVSDLRPLDEADQGDLVDTDVKR
                     EVAAARAIEALRAKFGRTAVVRGIGMGADRPKPKGKS"
     gene            complement(14960..15244)
                     /locus_tag="PK812_03995"
     CDS             complement(14960..15244)
                     /locus_tag="PK812_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008191910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3572 domain-containing protein"
                     /protein_id="HRJ68748.1"
                     /translation="MISTLKKTLDPEAVAIQALAHIAADAERLARFLAMTGIEPETLR
                     EAAREPGFLSQVLEFVCSDDSGLLAFAANAGLAPDAVARARLALAGPVAN"
     gene            15378..15749
                     /locus_tag="PK812_04000"
     CDS             15378..15749
                     /locus_tag="PK812_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018045229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRJ68749.1"
                     /translation="MNKTVLIVEDNELNMKLFNDLLQAHGIKTLMTSSGVEAIELARK
                     HVPDLILMDIQLPEISGIEVTKWLKDDEHLKSIPVIAITAFAMKGDEERIRESGCEGY
                     LSKPISVGKFVETVRAYLGTG"
     gene            15814..17187
                     /locus_tag="PK812_04005"
     CDS             15814..17187
                     /locus_tag="PK812_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PleD family two-component system response
                     regulator"
                     /protein_id="HRJ68750.1"
                     /translation="MTARILVVDDLFPNIKLLETRLTAEYFDVLSAMNGMQAIEICES
                     GACDIVLLDVMMPGMDGFEVCRRLKNSPTTAHLPVVIITALDQPSDRIKGLEAGADDF
                     LTKPVNDVALLTRVRSLARLKTLTDELRARAATSAQFGQPDALASAAAEDGLNGRVLL
                     VDDRSNSVERMVGSLQQVHTVCVETDPQAALMRAAEENHDLAIISLGMAGFDALRLCS
                     QLRSLERTRNLPVLVLADLDDDQRVLKALDLGVNDYLTRPIDRNELVARVRTQIRRKR
                     YADRLRQNVQSSMEMAIIDPLTKLHNRRYLTSHLAAMLNDGMARGTAVTLAVIDVDFF
                     KKVNDTYGHDAGDEVLVETAARLRRSLRGIDILARFGGEEFVVAMPDTDRFAGVRVAE
                     RLRASIEGQPFTIHKGARQISITASIGVATASEPTTTADTLVKRADEALYEAKRSGRN
                     RVIEAAA"
     gene            complement(17517..17684)
                     /gene="rpmG"
                     /locus_tag="PK812_04010"
     CDS             complement(17517..17684)
                     /gene="rpmG"
                     /locus_tag="PK812_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HRJ68751.1"
                     /translation="MAKSATIKIKLVSTADTGFFYVTKKNAKTATEKLSLKKYDPVAR
                     KHVEFKEGKIK"
     gene            complement(17792..>18458)
                     /locus_tag="PK812_04015"
     CDS             complement(17792..>18458)
                     /locus_tag="PK812_04015"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRJ68752.1"
                     /translation="APYLVTSALFLAALAPLALAPKSPPDLAAHGRMRLGPLVAAAPV
                     ATIAALVFGAVETGAFSQLPLYGLRIGLSEPDAALLITWTVLGNLAFQIPLGALSDRI
                     DRRIVLFACSLFGALGCALMPAVSTMPLPQAVLLFFWGGIIGAIYTVGLAHLGARFSG
                     ADIAQANAAFVMLYSFGLIAGPPYVGAGMDALGSVGFPLTMGALIAAYAALVGWRLAR
                     GQA"
BASE COUNT         3371 a         5687 c         6036 g         3364 t
ORIGIN      
        1 cgcagggaac acggcgtgat ccgctcagca atcgcaattt cgatctgaac tcgcctcaga
       61 ccgttccgcc cttgcgctga aggctggcga gccctagtcg ggtcggaggt gaacggaaag
      121 gcgcggactg gccatgtcga ttcgcagcat gacgggtttc gcgcgggcgc agggcaccgg
      181 cgcaggcttt tcctggacgt ttgaagcgaa gagcgtcaat ggtcggggcc tcgatgcacg
      241 gtttcgcctg cctgcggggt tcgacatgct cgagccggac atgcgttcac gcacccagaa
      301 ggccctgacc cgaggcaacg tcaacgttac cctgacggtc cggcgcgacg cctcgagcac
      361 cggcgttcgc atcaacgaga cgctgttcgc gcacctggcc gaagcggcgg agcgcctcgc
      421 ctggaccgct aagctgccga gcatgacggc cggcgacatc ctgcgcctgc cgggtgtgat
      481 cgagagcggc gatgcggcgg aggagggacc gccggagtcc gtgattgcgg cagtcctgac
      541 gagcttcgat gcggttcttg ctgatctggt cctctcgcgt cggtccgaag gcgcggcgct
      601 ggagcggatc atcgccggac agatcgatgg catcgagacc ggtatcggca ccgcagaggc
      661 tgcggatagc cgccgatctg acgccatccg ttcccggctc gaaacgcaga ttgctgccct
      721 gctgggcacg gcggaccggt tcgatcccga ccgactgcat caggaagcga tgctcatcgc
      781 ggcgcgaatc gacgtgaagg aagagatcga ccgtctgaag gcgcatgtcg cagcggcgcg
      841 cgccctgctg gcgagcggcg aggcggtcgg gcgtaagctg gatttccttg cgcaggagtt
      901 cgtgcgcgag gcgaatacgc tctgttcaaa ggccaacgac attgccttga cgcgtgccgg
      961 actcgatctc aagacatggg tcgagcagtt ccgcgaacag gttcagaaca tcgagtgagc
     1021 gcatgacgaa tatcctcgcc agtcgccaga tcaccgaccg tcgcggcgtg atgctggtga
     1081 tttcctcgcc ctccggcgcg gggaaatcct cgctcagccg cctgctactt caggaggaaa
     1141 aggaacgcat ttccctctct gtgtcggtta cgacccgcaa gcggcgcgcc agcgagatcg
     1201 atggcgtgca ctatcatttc aggtccgtgc cggagttcga ggtcatgcgc gagcgtggcg
     1261 aattgctgga atgggcgcaa attcacgata atttctacgg cacgccgcgt ggaccggtgg
     1321 aaaaggcgct cgccgatggg catgacgtgc tctttgacat cgatgtgcag ggcacgctgc
     1381 aactgtacga aaagatgcgg gaggacatgg cgacggtgtt catattgccg ccatcgattc
     1441 ccgaactgca caatcgcctc aagcgtcgcg ccgacgatga cgaccaggtg atcctgcgcc
     1501 gcctgaaaac cgcgctttcg gaaatccggc actggaccga ctacgactac gtgctcgtca
     1561 atgatgatct cgatcgctgt ttctttgaac tccagtccgt tttggcggcc gagcggttga
     1621 agcggtcgcg caggccggcg ctggccggac tggtgaagac gctggacaag gacctgacgg
     1681 cgctgctcgg ggcctgagcc ggctcagggg caggcaaccg cgttggcgat cgtcacaaat
     1741 tccgcgaccg acagggtctc ggcccggcgg gttccgtcga tgccggcgcg ctcaagcagt
     1801 gccgcggcat cgccaagcgg cttcagactc tgacgcagca tcttgcggcg ttgtccgaag
     1861 gccgcggcgg taacgcgttc cagcgcctgg acctgacagg gcaggggcgc cggattgggc
     1921 accagttcga cgacgcttga ggtgaccttc gggggcggcg tgaacgcaga cggcgccaca
     1981 tcgaacagga tgcgggacct cgcgcgccag cctgcgagga cgccgagccg gccataggac
     2041 tccggtgtct gcggcgttgc cacgatccgt tccgcgactt ccttctggaa catcagtgtc
     2101 aggcttttcc agaagggtgg ccaggtcggc gctcgcagcc agccgatcag cagcgccgtt
     2161 ccgacattgt agggcagatt tgccacaatc cgcgtggtgc gtccggcgat cagccggtca
     2221 ggactgatcg ccatcgcatc accactgatg acgtccaggc gatccggata gcgcgctgcg
     2281 atctccgcca gcgccggcag acagcgctca tcgcgctcga tggcgatcac acggtcggcg
     2341 cccgctgcaa gcagggcgcg tgtcagtccc ccagggcccg ggccgacctc gagaacaaca
     2401 tggccttcga gcggtccggc agcgcgcgct atgcgactgg tcaggttgag atcgagcaga
     2461 aagttctggc ccagcgcctt gcgcgcaacc agaccatgct cgcgaatgac ctcgcgcagg
     2521 ggcgggaggt cgtcgatctg ggccacgtgt tgaagtcttc agcggtactg gatgacggcc
     2581 gtcttgcgca ggttcttcag atacccttcg gcaagcgtcg cgttccgttt gctcgcgact
     2641 tcctgttctg ccgtggtgcg cacggcggac tgatcgatgc gctcgatctt ctcgcaaacg
     2701 gcatagagtt cgatgccctg cgtcgtccgc atcggaggcg acatgcgtcc gaccggcgtg
     2761 tccgcgaaga tcttgttgag ctggggcggg atctggttcg aggaacgctt gacgggagcg
     2821 cggatcgcca cgtcacgcag cttgcgcagg tcgtcgatgc cggtttcgca ggaactgaac
     2881 gctccccggg ccgcctgtgc ctcacgctcg cgctggccaa cgccttcgcc ggtgcggggc
     2941 acgacaaaga tgacggtcac gatgacgtag tccgtcacct tggcagcacc ctcgttgagc
     3001 ttcttctgat agacctcgcg aacctcgctg tccgcgccaa ccgcagccgc tgccttgcct
     3061 ttctgcgaca gggccagttc ccaggaataa tcggcgcgca gcttcagctt gtaggcctgc
     3121 gtatcgacgc cggcgcgcga cagattggcg atgaactcgg ggagggtctg attgttctgc
     3181 atggcgatcg aggacatgcg atcctcgata ttgtcatcct tgagcctgta gccgatgcgc
     3241 cgcgcctcga tgatcttgag ccgatcatca atgagctgtt ccagggcctg cttctgagtc
     3301 aagcccgtgc gtgccaggac ttgcatgatc cgtatcctat gctcgacgtc aacggaggtg
     3361 atcggctctc cattgacgaa agcaaggacc gtctgcgctg cggcaggggt gatagctgaa
     3421 tatgttgcgg cctggatcgc cagggcaagc aggacaccga tcacatagcg catgggtgtt
     3481 gacctcactg gccactcgac gagtcgtttt gcgtcgagat gccgctcgtg ccccgatact
     3541 ggacctgccc gagatgtttt agttcaagcc ggaacatgta gaccgtgccg atcttgttcg
     3601 tgccgctcgt caccatgtcg gacgtctgcc gcgcccatga cacaccaaaa tcgatgcact
     3661 cgtccctgta gccgagtgtc gtggagatgc ccgacacccg gaacggcgac gaattgtatc
     3721 gaggatagag gtcgggatag gcgacatggt aggatcgatc ctgatgccgc cgatccatgt
     3781 cgaatgtgat gctgctgctc gcgtaccaat tgctcggcag gcgaagcgaa agcgtgctcg
     3841 accatccctc gcgcgggagg ttgtagccaa gcatcggttg gggagccagt cgtgcatagg
     3901 aggtcgacag cgagaccggc ccggttgacg ccgacgctcc gatctcgagg cgccgcaact
     3961 tgaacgtgtc ctcatcgaag cgcccacggg ccgaaaagtt caagttggcc acgggcgaaa
     4021 cggcgatgcg accaacaaaa tccgaccggt gcttctcaag gccgctgttc agaccggtat
     4081 tcgaggcatc cggagtcgaa tacgagttcc gcccgccgag ttggttggac tgaccaacca
     4141 ggacgttcat cgaggcgccg ctcgcggccg tcatcgtgta ctggaagccg tagttggcgc
     4201 gcacgccacc ttcggttcgg tcatagcccg agaacttgtt cgttgaaaac agaaccgtgt
     4261 cgtcgaacac gaggctctgc gcatcctcgt tgggcatgta ggtcgcccga cgctcattgg
     4321 gacgtgcgat gatctgggca atcggctcga tctgatgggt gatgttgcca gcgaaggcaa
     4381 taaacgggaa ccggtaggtc acgcccaccg tcggcatcac gcggccgacg acgtccgaac
     4441 tgtcgcgcac gaagttcgtg agcttgtcat tgccccaaat cgcgttgtcc agcggcgcat
     4501 tgacatagcg ggttgtgtcc aggttgtagg acaggacatc gccgcgcacg ctggcaaagg
     4561 gtgtccagac ttgcccaacc ggatcgatga aggagcgacg ccagctcagc gccacggtcg
     4621 cgcgggtcga attgccagac atgccacgca agatgcagtt cgtgcgcgta taccagttac
     4681 agccctcgta gagcccgtag ccgaaagcgg ctccgttttg ccgataggcc gtgcccgaga
     4741 agatgttcgg cgatccggca tggccaacgg aattcggcgt cggcaatgcc tggaaatggg
     4801 ctgcggaccg cgacaggctc gtcaggttgg cggtcaactg cacttcgccg ccaatgaaac
     4861 ttgaggaaaa ccgccggtca tagtccagca cgggatgggc gtgtgcctgg ttcttcagcc
     4921 attccgcatt cgtcgcgggg gtgaaatgct gggcacgcag atcgaaccag cttcgctcgc
     4981 cctgtccgtt caggtagacc tgtgaaacga actcaccctg gtaggtgttc cggaaagcgt
     5041 cggtcttgat gctgtagttc cggaaaaaga accgatccgt cgctgcggcg agatcccagc
     5101 cccacttcca cttctcgttg atgtagaact ggccggtggt ttcgacgctg ccgcggaatt
     5161 tccggtcacc ggcgcccagg gggcccggca ggaatgcgtc gcgttcattc tggttgatgc
     5221 cggcgatgcg cagggtgtag gtgccggtct gcagccggtg gcggaattcg gcctgcccga
     5281 gaaagccctg acgcgaatag taggtcggcg tcagcaagac atccatgttg ggagcgagcg
     5341 cccaaaagaa cggcgccgaa acgccggttc caaggctctt cttgttggtg atcctgccca
     5401 tcaggaaccc ggtcttgcgg ttcacgctcg ggtcagggcc cgagaagaag ggcatgtagg
     5461 caatcggcac gccccagaac tcaagcgttg cctgttcgta atagatcgcc tgctcgctgg
     5521 tcttgtggat gattcgggcg gcgcggatct gccagagcgg cgggcgcttc gggtcggtct
     5581 tgcacggctc gcagggcgta taggttcccc gttcaaacac ggaggtctcg ccttcggtcc
     5641 gctcggcacg agcggcggag aagcgttgct tgtccggcga ctggctgcgc agcgaatcga
     5701 tgaagccgtt cttgaagtcg tcggtgagtt cgaaccgatc accgaagtac ttggtgccgt
     5761 tcgcttcgat gatgcgcgca ttgccctcag caaaaacgcg cttggacttg cggtcgtaga
     5821 tcacccggtc ggcttcgagc gtccgcccct ggtagtagag ctgggcgttc ccgaccgccg
     5881 agaccgtgtc cttctccttg tcgtagacaa gttcgttggc ctcgaccatc agcttgtcgg
     5941 tcggcttcgg ggctggagcc gctgacttcg ccttctgtgc ccaagcaggt ccaggcgcaa
     6001 cgcttgccgc gccggccaca aggagcgcca gaaccgtcag ggaacaaagc cgcaggccgg
     6061 ggcggatcat catccgtcct cccggtgcag aagcgcgagc gagccaagca tgccgccgat
     6121 cacgggggcg gcccatgcgg caaacaccgt cgagaccaat ccggcagagc cgaggtcttc
     6181 cacgatctgg gtcgctacgt acaatacgaa ccccgccgac acgccactca acaccattcc
     6241 cgcaaatcca cctgaccgca aaaaccttaa ggaaacggat gcagcaacca aaaccatcgc
     6301 ggtcagcagg gctggccgcg cgagcaatga ttggaacctc aggcgatacc gtgtcgcatc
     6361 gaggccggca agctccagtc cgcgcacgac cgaggggagt gcccagaagg acacagcctg
     6421 gctttgcccc accgaccccc gcaattgttc gcgcgagacg gaagtggcca gcagatagcg
     6481 ttccatctgc tgcggttcgt tgtcgacggt gagcacgcga acgttctcga accgccaatg
     6541 gttgcccaga tggatggcgc gctgcccttc gatccgctcc atgaacttgc cgttgggatc
     6601 gaaggaaaac gcggtcacgt ccaccaacga gaaggtgttc tcgtccagcc gggccgcgcg
     6661 cagaatcgtt tctccatcga ccgagcgttg acgcagccag acgccctttt cgttgccgcc
     6721 aacgccgcct cgcgaaaaca aggacgcttc gtaggcttcc gcgcgggccc tgaaatgggt
     6781 tgccaacggg ttgtacgcga ccgtcgcaag cagaccaatg agcgccgcga caatgatgcc
     6841 gggcagggag aactgccagg ccgaaacgcc ggcggcccgc gccacgacca gttccagccg
     6901 gcggctcagg ttcaggaagg tcgccagcgc gccgaacagc gctgcaaacg gcaggatctg
     6961 ctccgcggtc gcgggcgtgc gcagcagcgc gaggcgggtc agcgccaaag tggtcgcatt
     7021 cggcgcctca ccggagcggc gcatcaactc caccaggtcc agcgtgaata tcaggatgaa
     7081 gacggtcagg aacacgccga ggatcgcctt ggcgaaccgc agggcgaaat agcgaccgag
     7141 ggtgccgaca aggaacgtca tgaccggcgc agcttcatcg acaactgcgc catctgcgtc
     7201 acgatggcgc caggattgat ggcccggagc tgccgggtca ggccgaggcg gttggaaacc
     7261 gccgtgagtc catcgacggc tctgaccagg acctgcggcc tgtcctgcgt ccaccaagag
     7321 taggtggcgg ccatgccgac agccgcgatg ggcacgacat acatcatggc gatgccgaac
     7381 gaaccgcgcg cgccgagact ggatgcccaa aatccggcga tgcgcagcag caagaccgcg
     7441 atgccggcgg ccgccatggc cgcgccgcgt ccctgtcgcg tggtccgggc gctgccgagt
     7501 gcggcgaagg cgatggcgag catggcaaac gcatacagcg gttgcacgaa acgctcgtgc
     7561 agttcggcgc ggaagcggcc gatctgccat ttgacgtaga attcctcctt gtcgagcgtc
     7621 atcaactccc aggttgtccg ttcccggggc ttgtacacga tcttctcgtt ctcgccgccg
     7681 aactggtcca tgtcgaacgc atagcgttcg aaatagacga gcgcggattc cttgcgcgcc
     7741 ttgtcctgac gctgaaggct gcccttctcc agcaggaaat actgaaggcc gtcgacctcg
     7801 accatccgcc cgcgttcggc gaggtaggtc gtcaccatgt tcgcattgcg cctgtcctgg
     7861 aacatgatgc ccagaagtat gccgtttggc cctttttcct tgtagtgaaa ggttatcccg
     7921 gcatcgagcg tgacgaactt tccttcctgg acgaactggg tgatcaggtc ggcgcgcatc
     7981 ttcaccacca gatcgcgcag cgagcggaag ctctccggca tgaccttgat ggtgatcagg
     8041 ccgaccagca tggccgtcgc cagcgtcaac gtcaggaacg ggcgcaggat cttcatgggc
     8101 ggcacgccgg ccgcgctcat cacgatcagt tcgctgtcgc cgttgaactt gttcagggtg
     8161 tagacgatcg aaatgaacag cgcgagtggc gcgatgatca tgatcagggc cggaatcgag
     8221 agcagggtga tgtacaggaa cagcagcacc gactggccct tgccggtgat cagatcgagt
     8281 tccttcaagg cagacgaaat ccagatcacg cccgtaagcg cgcaaaggca gagcaggaag
     8341 gcgctgacgg ccgtcctgaa aatgtacctt tcgaggatct gcatcccttt agaccgtagc
     8401 ccccaatgct gccccgtgcc cggcgcatga tcgaatccaa gcgggcgttg aaggcattga
     8461 tatggcagag ccggcccaat gtccggcgca ttccgcgcgc gcgggcgaac tagccggcgg
     8521 ctgtggcaaa aaaacccgtg ttgaactcgc gggttagatg cctttttctg gtgatgtcac
     8581 gaatcggtcg cgtcgggtcc ggaatttccc gagcttcgcc gcgccatcga cctacaggaa
     8641 tcgtccatgt cgcgcggccc gagattgtct ttcgcctccc ccgattccgg cctccatccg
     8701 gtggttgtct tgttcgtcgg cgacgatctc gcgttgccaa aggccgccgg gcggctcggt
     8761 ccgggtttcc ccgccctgtt caagacagcc tgtgacgggg aaggctacaa gggcaaggcg
     8821 gggtcgacac tggatctggt cgctccggcc ggcgtgccgg cgaaccgcgt gatcgtggtg
     8881 ggaacagggg gcgacgggga ccgcgccaag ggcgactgga tcggcatcgg cggcaaggtt
     8941 atgggcaagg tgccggccgg ccgtgccgtc agcgtgctgt tcgatgcgcc cggagcggat
     9001 gcgccggcgg cggcggactt cgcgcttggc atcgggctgc gcgcctacag gttcgatcga
     9061 tacaagacga aaaagaagcc tgatgaagag aatggagcgg cgggcgacgt ctccgtgcac
     9121 ctgaccgact tcgccggggc caagaaggcg gtgaagagcc gggacgcggt gctggacggc
     9181 gtcaacatcg cacgcgatct cgtcaacgag ccgccgaacg tgctgacgcc gaaggaattc
     9241 gccgcgcgcg ccgcgcagtt gaagaaggtc ggcgtcgacg ttgaaatcct tggcgaaaag
     9301 gagatgagga agctcggcat gggcgccctt ctcggggtcg gtcagggctc ggttcaggaa
     9361 agccagcttg cgatcatgcg ctggaacggc accaaggatt ccaaggccgc gccggtggct
     9421 ttcatcggca agggcgtggt gtttgacaca ggcggcatct cgatcaagcc ggccgccggc
     9481 atggaggaca tgaagggcga catggcgggc gcggcctgtg tcgtcgggct gatgcatgcg
     9541 ctcgcggccc gcaaggcgaa ggtcaatgcc ctgggtgcca ttggcctcgt ggaaaacatg
     9601 ccggacggcg cggcgcagcg cccgggggac atcgtcacgt ccatgtcggg ccagaccatc
     9661 gagatcatca acacggacgc ggaaggtcgc ctcgtgctgg ccgatgtgct ctggtacgtg
     9721 caggaccggt tcaagccgaa gttcatggtg aaccttgcga cgctgaccgg ggcgatcatg
     9781 gtcgccctcg gcaaggaata tgccggcctc ttttccaaca acgacgacct cgccggccgg
     9841 ctgttcgagg cgggcggcaa gaccggggaa aaggtctggc gcatgccgct cggccccgaa
     9901 tacgacaaga tcatcgattc aaagttcgcc gacatgaaga attcgggcgg acgtttcggc
     9961 ggatcgatca cggcggcgca gttcctccag cgcttcgtca attctgtacc ctgggcgcat
    10021 ctcgacatcg ccggcaccgg catggatgcg gcctcgaccg acatcaaccg ctcctggggc
    10081 aatggctggg gcgtgcgcct gctcgaccgg ctggtggcgg atcattatga aggctagacg
    10141 cccagtctgg gcgcttttca tgctggccgc gtgcatcggc gcggtccatg cccagcagcc
    10201 gcgcccgcac cgcggcgtcg atatcgccgc tgattgcgcc ccgtgtcatg gcgtcgacgg
    10261 catcgccaag gagcgggacg tgccgcatct ggctggccag aacgccggct atctctacaa
    10321 ccagatgaag gcgttccagt ccggcaagcg gccgcacaag gagatgcggt tcatgtcgcg
    10381 cccgctcacc gaggccgaga tgcaggcgat tgcggagttc tacgccactc tgccgccgcg
    10441 ctgaccggca gacggtccgc cttccgcctc aagcagcgtg cctgcgcagg tcaagcttgc
    10501 gtctcggcaa ggtctggcat gcattttgat ccctgcgtcg ccaagcgggc cgacaacgct
    10561 ccatgaacca tgaaatggca cagacgctgt gcctttgcgg aacagggaac ggtcgccccc
    10621 ggaacgaact ggggtgacgg atgctgacga cggtactgaa caagagtgct ggatctgacc
    10681 tgacacggat gtccgggagc cagcatgccg ggttcaccgt ggcggtgcgt cgggacgtgg
    10741 ggcatgccgt ggccgtcgcg gcgcgcctgc tgcagggtgg cagcgccact gtctttcagt
    10801 ccgccgtgtg ggtcgaggct tttgcccgaa ccgttgcggc ggagcgcggg tgcgcctttt
    10861 tcatggtcga ggttcgcgcc acggaaagtg gccgcccggt catgctcctg cctttccttc
    10921 tgcgggagcg tcggggattc aggctcctgg aagtcccgga tttcgggctc gcggactacg
    10981 tggcgccgat gtttgcgcgc ggcttcgatc cggatcgcca gacgatgcac cggatctggg
    11041 cgcaagtccg ggctgttctc ccgaaggccg atgtcgtccg cttcgtgaag atgccgggag
    11101 aggtcggcgg tcgcgtgaac ccgctggttc ggctttccgg gacccgttgc agttccttga
    11161 ccgcctgggg tgtagacttg gggcctgttg agacgatctg gcgcgatgcc atcgcctgcg
    11221 acaagcgtcg ggcggatctg gacgcccgct ggcgcaagct gaacaagcgt ggcgtggtcg
    11281 agttccgaac ggcatcgacg gaagcggatg cggatgcctt cttcgagacc atggtgcagc
    11341 agcgctcgga acggttcagg gcgctgaatt ttcccaacgc actggacaag ccggaaatcc
    11401 gtgacctcta ccggtctctg ctgcgcccgg gcgagccggc atctcctgcc gtggtccagg
    11461 ccctgctggt cgatggccgg atcgttgcga cgggctacgg tctggtcggc aacggcgcgt
    11521 tccacatgat cttcccgacc ttccaggccg agacctggcg caactattcg ccgggtctgc
    11581 aactcttccg caagagcatg gaatggtcgg cccgaaaggg gctccgatac tacgatttca
    11641 cggtgggttc agaaggtttc aaacgcgatt tcggggccag agaacagcca ttgttcgagc
    11701 gctacgaagc gctgagcttc gccgggcttc tgccagtcgg ctacgtcgcg ttgcaccgtt
    11761 tcattcgcac acaaccgtgg ctggcatcgc tcttgcgtgc cgtcaagtcg atgttcatgt
    11821 ccggaggccg ctgaaatgag cacattcgtt gccgattcga ccgccgttgc gccgcatcgc
    11881 tacgccgtcc tggatgcctg gcgcggcatc tgcgcgctgc tggtcacggt ggtgcacatc
    11941 ccgctggcgc atcgctggct cggcgcgcag tggttcgaga acatgcagat gttcgtcgat
    12001 ttcttcttcg ttctgtcggg atttgtgatg tgccatgcct atgggcacaa gctcgacgag
    12061 ccggtccgct acaacagctt catgatccgc cggttcgggc gcatctggcc cttgcacgcg
    12121 gcaatcctgg cgggatttgt cctgctggaa ctggtaaagc tgggtgcgca ggtcgggctc
    12181 aagctgccga tggatggcga ggcgttcacc ggaccgcgtt ccgtcgcgac gctggtgtcg
    12241 aatgtcttcc tgacccagtc gttcaacctg catggcatga cgagctggaa cggaccggcc
    12301 tggagcatcg gggtcgagtt ctacgcctac atggtgttcg gactggtcgt gttctacgcc
    12361 gggatgcgga ccgctgcctt tgcggcgctg gcaacggtcg ggctcgccgg cgtggggctc
    12421 tttgctccaa actggatatt cacgacacac gactacgggt tcttccgctg cctgttcggc
    12481 ttcttcactg gctgcctggt ctatcaggcg gtgcagaagg gacgaccggc ctggtccggg
    12541 gtcaccgcgg aagtcgcggc tgtgattgtc cttgtcgcct tcatgctgct gaccggacgg
    12601 aacgccacaa gtcttgccgc gccgatcgtt tttgccggcg tcgtgtatgt cttcgcgttc
    12661 gagcgtggcc cgctttccgc agccttgcgc acggcgcccg cgcaagcgct cggcctgtgg
    12721 tcctactcga tctacatggt gcacatgctg atcttcgcgg tcgagaagat cgccctttcg
    12781 gtgggcgaaa aggttgcggc gctcaagctg acctcggtgg tgacaccgac cggcaagttc
    12841 tggacgttca acaacggcgt gctggacacc ggcttcttcg cgatgcagat cgccctgacg
    12901 ctgcttgctt ccgccatcac ctatcggctg atcgaggaac cggcacgacg ctggttcgcc
    12961 cgtctggccg acgcctacga gcgcaagcat gccccgtcgc cgcagggagg cctgctggcc
    13021 acgcggatgg cgggcctcgt gcgctgaggc ggctttcccg cgatgcccgt cgaggggccg
    13081 ggacgccggc cctttcggct ggacgctccg ctcggctata agacggacat ggtcgaggtt
    13141 tggttttatc atctgacgcg cggatcgctg gaagacacgt tgccggcgct ggtcgaacgc
    13201 acgcgggcac gcggctggaa ggcggtgatc cgggtcggta cggccgaacg catggcagcg
    13261 ctcgatgatc tcctgtggac ctacgacgac gagagtttcc ttgcgcacgg cacggccagc
    13321 gagccggatg cgcaggtgca gccagtcctt cttacgacag ccgacggact gccgaatgac
    13381 gcggacgtct tgtttcttgc cgatagcgcg ccgctgcctg gcgaatggcc gcgcgaacgc
    13441 gtgatcatgc tgttcgatgg cgcggatccg gacgcgctcg aacatgcccg cgcagcctgg
    13501 aaggaagcga taacactggt gcatccggtg cagtactggc aacaggacga ctccggtcgc
    13561 tggtccagga aggcttagtg cgcccgtccc ttcgcgatca cggttctgag tggcttgggc
    13621 ctgttcgagc catggccggc agcgggcatt ctcaggactt gcccttaggc ttcgggcggt
    13681 ccgcccccat gccgatgccc cgcacaacgg cggtgcgacc gaatttcgcc cgcagagcct
    13741 cgatcgccct ggccgcggcg acttcacgct tgacgtcggt atccacgaga tcgccctgat
    13801 ccgcctcgtc cagcggcctg agatcactga cgccgacccc cagcagacga tagcgtaccc
    13861 ccgtcagttc gtgcctaagc aggtctcgcg caacgtcatg gatgcggacg gcgagcgctg
    13921 tcggcggcag gcccgagcgt gcgcgggtca cgaggcggaa gtccgccgtc ttcagcttca
    13981 cagtgatcga ggcgccgcat acgccggaat gcctcagacg cgcggcgact ttctcgcaca
    14041 ggcgccagag gatcggagcg aggtccactt ccgtcgaaag atcatcgttg aacgtggtct
    14101 cgttcgagac gctcttgatg gggtgggagg gcgtgacccg gcggctgtcg atcccgcgcg
    14161 acagtctgaa aaggcgtccg ccctcggagc cgtagcgccg atacaggtct gcctccccca
    14221 cgcggcggag gtccgcaatc acccggattc cgtcacgggc gaggcgatcc tgcatggcgt
    14281 gcccgacacc ccagatcaga ccgacaggcc tgggcgcaag gaagtccagc gcctcatccg
    14341 tcccgataat cgtgaatccg cgcggttttt cctgatccga ggcgatcttg gcaaggaact
    14401 tgttctgcgc cagaccgacc gaaaccgtca gccgcatgtc gctttcgatg cggcgcgcga
    14461 aacgcgccag cgtcacggcg gggcttgcac catgcaggcg ctcggttccg gacaggtcca
    14521 ggaaagcttc atcaatggac accgattcca ccagaggcgt cagttcgagc atgcgggctt
    14581 tgatctcgcg gccaacggct gaatagagct ccatccgggg cttgatcacg acggcttcag
    14641 gacaggcctt cagcgctttg aacatgggca ttgcggaacg cacaccgaag gtgcgggcta
    14701 tgtagcaaca ggtggaaaca accccgcgct tgccgccgcc gacaatgacg ggcttgtccg
    14761 ccagcgtcgg gttctcgcgc ttctcgacgg ccgcgtagaa ggcgtcgcag tcgacatgcg
    14821 cgatcgccag ccgatcccgc tccggatgcg tcaacaccct cgggctgcca cagccggggc
    14881 agcgcgcgac gtggtcgttc gagctggtgt cggtcagaca atctcggcag aggaagcgca
    14941 tggcgccctc ttgcaggaat cagttggcga ccggtccggc caaggccaga cgcgcacgcg
    15001 ccactgcatc gggagccagg ccggcattgg cggcaaacgc caggagaccg gaatcgtctg
    15061 agcaaacgaa ctccaggacc tgcgacagga atcctggctc gcgagctgcc tcgcgaagcg
    15121 tttccggctc gattccggtc atcgcgagga agcgcgccag gcgttcggca tcggccgcga
    15181 tgtgggcaag cgcctgaatc gcaacggctt ccgggtccaa ggttttcttg agagtcgaga
    15241 tcattccgcg agcttcggcc tgaacgcggg attccgcaat atttaatcgt tacctgctag
    15301 gaagcagcca tgagtggtgc ggatgcggtt ccgggtgacg gggcgatgcg cgttggaagc
    15361 cacgctgggg cagggcaatg aacaagacgg ttctgatcgt tgaggacaat gagctgaata
    15421 tgaagctctt caacgatctg ttgcaggcgc atggaatcaa gacgttgatg acgtcgagcg
    15481 gcgtcgaggc gatcgaactg gcgcgcaagc acgtgcccga cctgatcctg atggacatcc
    15541 agcttccgga gatttccggg atcgaagtga ccaaatggct gaaggacgac gagcacctga
    15601 agtcgattcc ggtaattgcg atcaccgcct tcgccatgaa gggcgacgag gaacgcatcc
    15661 gcgagagcgg ctgcgaaggg tatctgtcaa agccgatctc tgtcggtaag tttgttgaaa
    15721 cggtgcgcgc ctatcttgga accgggtgac gctgcgtacg ctggctggtc aggcggccca
    15781 ttggccacag ccgagaatga gtaggggagt gccatgacgg cgcgtattct tgtcgtcgac
    15841 gatctgtttc cgaacatcaa gttgctggaa acgcgcctaa cggccgagta tttcgatgtg
    15901 ctgagcgcca tgaacggcat gcaggcgatc gaaatctgcg aaagcggagc ctgcgacatc
    15961 gtgctgctcg acgtgatgat gccgggcatg gacgggttcg aagtctgtcg tcgtctgaag
    16021 aactcgccaa ccacggcgca tctgccggtg gtgatcatca ccgcactgga tcagccgtcc
    16081 gaccggatca agggcctgga ggccggcgcc gacgacttcc tgacgaagcc ggtgaacgac
    16141 gttgctctcc tcacccgcgt gcgttcgttg gcgcgactga aaacgctgac ggacgaattg
    16201 cgcgcgcgcg ctgcgacatc agcccagttc ggccagccgg atgcgttggc ttcggcggct
    16261 gcagaagatg gcctcaacgg ccgggtgctg ctcgtcgacg accggtccaa ctcggtcgag
    16321 cgcatggtcg ggagcctgca gcaggtgcat acggtatgcg tcgagaccga tccgcaggca
    16381 gccctgatgc gggccgccga ggaaaaccac gatctggcga tcatcagcct cggcatggcc
    16441 gggtttgatg cactgcgtct gtgcagccag cttcgctcgc tggagcggac gcgcaacctg
    16501 cctgtcctgg tgcttgccga tctcgacgac gaccagcgcg tgctcaaggc gctggatctc
    16561 ggcgtcaacg actatctgac ccgccccatc gacaggaatg aactggtggc gcgcgttcgc
    16621 acgcagattc ggcgcaagcg ctacgcggat cggctccggc aaaacgtgca gtcgtccatg
    16681 gagatggcga tcatcgatcc actgacgaag ctgcacaacc gtcgctatct cacgagccac
    16741 cttgcggcca tgctgaacga cggcatggcg cgcggaaccg ccgtgacgct cgcggtcatc
    16801 gacgtcgact ttttcaagaa ggtcaacgac acctacggcc acgacgccgg tgatgaggtc
    16861 ctggtggaga ccgccgcacg gctgcgccgc agtcttcgcg gcatcgacat cctggcgcgc
    16921 ttcggcggcg aagaattcgt cgtcgcgatg ccggacactg atcgcttcgc gggtgtgcgt
    16981 gtcgccgaac gcctgcgcgc atcgatcgaa gggcagccct tcaccatcca caagggtgcg
    17041 cgacagatca gcatcaccgc ctcgatcggc gtcgccaccg cttcggagcc gacgacgact
    17101 gcggacacgc tcgtgaagcg cgccgatgaa gcgctgtacg aggcgaagcg ctcgggacgt
    17161 aaccgggtga tcgaagcggc cgcctgacgg ccgcatttat cttaatttcg ccaagacctt
    17221 aaggtaaacg gcagatgaag gcgattcccg tcgcctgaag ccttgccgtg cacgcaaggg
    17281 cgtgtaattt ccctacaacg gcgctgcgtt ctgcgtacaa gctcgcgccg agcaatagcc
    17341 ctgcggagat gcctgggtcc gccgcaactc ccaggtccgt ggggcttggc cggcctggag
    17401 acggattgga gtggcctcct cgtccgtctc cggccggccg cctcttgcct ctttcaacaa
    17461 tcaacaacaa aaaaggcgcc ctgcggcgcc tctcactctg ctttctcagg ccgatcttac
    17521 ttgatcttgc cttccttgaa ctcaacatgc ttgcgcgcga ccgggtcgta cttcttgagc
    17581 gagagctttt cagtcgcggt cttcgcgttt ttcttggtca cgtaaaagaa gccggtgtcg
    17641 gcggtggaaa ccagcttgat cttgattgtc gcggacttgg ccatcggtct caatcccccg
    17701 gcctgtcgcc ggctttcggg tcaaaagata agggccggac gttcgcacgc ccggcacgtg
    17761 ccggcgctta tggcgactgg gggctgccaa gtcaagcctg tccgcgtgca agtctccagc
    17821 ccacaagcgc agcgtaggct gcgatcaggg cgcccattgt cagcgggaag ccgacgcttc
    17881 ccagcgcatc catgcccgcg cccacatagg gcgggccggc aatcaggccg aaggaataga
    17941 gcatgacaaa ggcggcgttg gcctgggcga tgtccgcgcc gctgaacctt gcgccgaggt
    18001 gcgccagtcc gaccgtatag atcgcgccga tgatgccgcc ccagaagaag agcaggacag
    18061 cctggggcag gggcattgtc gacaccgcag gcatgagtgc gcagcccaat gccccgaaca
    18121 gcgaacaggc gaacaggaca atgcggcggt caatgcgatc ggagagggcg ccgaggggga
    18181 tctggaacgc aagatttccc aggacggtcc atgtgatcag caaggctgcg tccggttcgg
    18241 aaaggccgat gcgcaagccg taaagcggca actggctgaa cgcgccggtt tccaccgcgc
    18301 cgaacaccag tgcggcgatg gtcgccaccg gtgcggcggc caccagcggt ccaaggcgca
    18361 tgcgaccatg ggcggccagg tccggcgggg attttggcgc caaagccaag ggcgccagcg
    18421 cggcgaggaa cagcgcgctg gtgacgagat agggcgcc
//
LOCUS       DAOMXN010000026        17844 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_1801_length_17844_cov_10.447186, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000026 DAOMXN010000000
VERSION     DAOMXN010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 17844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17844
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1801_length_17844_cov_10.447186"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            213..1382
                     /locus_tag="PK812_04020"
     CDS             213..1382
                     /locus_tag="PK812_04020"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HRJ68753.1"
                     /translation="MLIAHDPVRAFVPYPATAVAGTASGPLASLSFGVKDIFDVAGYP
                     TGCGNPVKLAESGLRATHAPAVQALLDAGASFLGKTHTEELAWSLYGTNVHFGTPVNP
                     AAPSRVPGGSSSGSASAVAAGLCDIALGSDTGGSVRAPASFCGIYGLRPSHGSVSLSG
                     CMPLAPSFDTCGFFARDAATMLATGRVLIRDAHVPATRLLIAADLFDRLAPAVTDALA
                     PRLAAIHALLGKGETTRVYTQPIDPLYTAFRHLQSREVYAIHGGWVEKHHPPLGPAIA
                     GRFDFARAVTDRQVSTARAMRIEFAAHMQALLANGGVLVAPTVPGPAPRLDDSEDTLD
                     RYRHEAMVFLQPAGLAGLPQLVLPAGTVDDAPVGISLIGARGSDLALLELADRLG"
     gene            1544..2809
                     /locus_tag="PK812_04025"
     CDS             1544..2809
                     /locus_tag="PK812_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRJ68754.1"
                     /translation="MRKSLLGAVALAALMLPGAAYAQSGTIKIGILVALEGAFAVGGQ
                     DGVRNVELALKQVNNTIAGKKVETVVAPTDTKPDTAVRMARKLIEQDKVDLIIGPLSG
                     SEGIAMRDYAKTIPGNVVINGVSGALETTWVDPAPNFYRFNLDGGQWGYGLGSYAFKT
                     KGYKKVAAITADYSFGYTNFMGFAVDYCKAGGDIVHRFWQPLGQADFAGIIAQLPDNV
                     DAIYLGLGGTDAINFLNQYQQAGEKTKLIGGTILADQTVLTARGRAKEILKGTPVSGP
                     FATDDSSPAWQAYMKLYQDAWPANQRLPSPSLFGLGYYTATLAAIKGLEAAGGDLSNG
                     QAKFKEALNKLQLDSPVGKISLNENRQASGTVFINEVVDGPDGNLTTKNVAKFENVTQ
                     TLGMTVAQFRALGLPSRTVVDCAKLRSGG"
     gene            2918..3973
                     /locus_tag="PK812_04030"
     CDS             2918..3973
                     /locus_tag="PK812_04030"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRJ68755.1"
                     /translation="MTLISYLSRTHFAEAAIEDALPEELGQVSRALLLVDDDDAANLA
                     ASRVQDALTGISLSVVRARSRVAPAVMIARLLEALTDIQSNTLIAVGGPEAIGLARLC
                     AERARRSGAHLKVVAVPAGISDFGLSRRVRSSQGQYLACPRPDCIVADPTVLKPVESR
                     RLAASAMEVLVHAVEAYASPGYNPPADAMAADAVRRITHWLPRALADPSNSEALRELL
                     ASALTAGLALEKAFGGVDAFAVPIHAELGSGLLHGAVHGPLMAAMAEFNGEAVGERYT
                     TMAGYFANTDHRAHFSAEIGAFARNVGMPATLREIGVDRSQLGRLAEVAANDPAALAN
                     PRRLTSGDCRRILEAAW"
     gene            4095..4931
                     /locus_tag="PK812_04035"
     CDS             4095..4931
                     /locus_tag="PK812_04035"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRJ68756.1"
                     /translation="MSLAHAVAHGAFGRACLYELDRPMVPHAHREGHLIFHVSGPAAT
                     VVVDGKVLPLDPLRATAISPWQPHHFVPISRRETTLTLVLYIRPGWFVDASPTAFEIL
                     RFGRSTVEMSDHIRRLVLETARLLGAHGGEHGMFEDRLGALTQASFDQTWQWTGNADA
                     RAQRPAALRDFRLRRAINILSDNLSETLDLAEVSRASGLSRPHFFKLFREQMGLPPTI
                     FINALRMERAIERLARGEETVSEIGLDLGFATPASFSRFFIANGVVPPTAYRRSVIGL
                     AH"
     gene            5073..6089
                     /locus_tag="PK812_04040"
     CDS             5073..6089
                     /locus_tag="PK812_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRJ68757.1"
                     /translation="MDDFIRRHPFWSLVAILAVAALLWLVLAPWPPELVALWGRKQIF
                     LNALLGGITLGALYFLVAAGFTLIFGLMRVVNLAHGSLFLIGGYLGYEIATASGSWLL
                     AFPIVFVIVAVIGLLMQRFIFSKMEGEELRQTLVSIGLSIVFADLMLWWWGGQSYSIL
                     APQFLQGPMELPIINNVNATTGAVTWLRYPAVRVWILVAAIITGLAMWLVLNRTQLGM
                     LIRAGVDDREMLAASGVRIQLVFLAVFGFGAGLAGVAGIIGGTFQSLSPGEDTRFLLA
                     SLVVVIVGGMGSIAGAALGALIIGVTEQMGFVYAPTYSVVFTFLIMAAVLAFRPQGLL
                     GTGR"
     gene            6144..7154
                     /locus_tag="PK812_04045"
     CDS             6144..7154
                     /locus_tag="PK812_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRJ68758.1"
                     /translation="MWFERIPVVWWIVGLALLLLPLVANNFWLFQVMGWSFILGMIGL
                     SLMFLAGYGGMVSLLQMSIAGMAGYMVAILGQSGTATVSLGLAWWIVLPTALLIAALF
                     ATIAGALSVRTEGIYTIMITLAIAAAFFYFTRQNYTIFNGYSGFNLVLPPKLFGVNWR
                     DPIPFYYLSLGCATLSYGAVVYIARSPFGLALQGVRDNPRRMAALGFNVTAHRVAAHA
                     LASVFASVGGILLVWQNAQIAPGTIGISAAIDVLVVAVVGGMRRPLGPFIGALIYVVL
                     QVFSPDILGFFGFAADRFKLIIGLGFLAVVLFSPDGVLGIWDHWRAREARRKAEAPGG
                     WA"
     gene            7178..7954
                     /locus_tag="PK812_04050"
     CDS             7178..7954
                     /locus_tag="PK812_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ68759.1"
                     /translation="MSAIGARNASSGNALELRGITKMFGALAAISDVTLSVRPGERRA
                     VLGSNGAGKTTLFNCITGDFLPSAGFIRFFGEDVTIFPPHERIRRGLRRTYQISSLFT
                     GLSVMDNVYLACRGVSRNRFSLIRPRRDDALMHAAEILVEAVHLTAERDRPVGELAYG
                     QQRQLEIALALSGAPRFILFDEPAAGLSPTERRDLVHILSSLPAHIGYIIIEHDMDVA
                     LRVVESVTMMHNGRIFKEGRPEEIEDDPEVQELYLGGGHE"
     gene            7947..10193
                     /locus_tag="PK812_04055"
     CDS             7947..10193
                     /locus_tag="PK812_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ68760.1"
                     /translation="MSERAPRVPGASQPILQLQGLNVFYGRSHALQGVDLTLHSGVLS
                     VVGRNGMGKTTMCKTIMGLVKATSGSIRFMGEELLGRTPAEIARKGIGYVPQGRRLWR
                     SLTVDEHLRLMSTGKGSWTPERIYEVFPRLAERRRNGGGQLSGGEQQMLAISRALLMN
                     PKLLIMDEPTEGLAPVIVAHVEETLVRLADEGDVSILVIEQNIGVATQMSDPVAIMVN
                     GRIHQVIASQTLAADRDLQQRLLGVGRHSHDETDVAAGDAGRARSADPAQPVNQGPIR
                     VYNANPTLPTRWSQDIPAARIEAAARTISRPTLATIDGRRVGDISPLAARTPQEPHVI
                     VAGTLDTKGDELRYIRDIIKAAGLRTRLVDLSTNGRNQGADVSAQEVALASPKASAGI
                     ASGDRGTAVAAMTEAFRAWVERQQNVAGIIAAGGSGGTAIATAGMQVLPFGVPKIMIS
                     TMAAGNVAAYVGPTDITMMHSVTDVQGLNRVSRAILGNGAHAMTAMARGWIERQASRN
                     TPVAGLRPGPERPLVGLTMFGVTTPCVQQVTKLVEPDWEPLVFHATGIGGRSMEKLID
                     SGLLQGVIDVSTTEVCDMMMGGLLPATDDRFGAVIRTRLPYVGSVGALDMVNFAAPDT
                     VPAQYRGRLFYPHNPQITLMRTTVEENRRMGRWIGERLNQMEGPVRFLIPEGGISALD
                     APGKPFWDPAADDALFSAIAETFRTTANRQLIRLPHHINDPAFAAALVQHFRSLHQVR
                     ARGSSSRT"
     gene            10197..11081
                     /locus_tag="PK812_04060"
     CDS             10197..11081
                     /locus_tag="PK812_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011927606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate hydrolase family protein"
                     /protein_id="HRJ68761.1"
                     /translation="MPRFSRSELLARYRAMIARGEPIVGGGAGTGLSAKCEEAGGIDL
                     IVIYNSGRFRMAGRGSLSGLMPYGDANAIVMEMAAEVLPVVRKTPVIAGVCGTDPFRM
                     MDVFLDEVKRVGFSGVQNFPTVGLIDGVFRRNLEETGMGYGLEVDMVALAGRRDLLTT
                     PYIFSEADAIAMAGAGADILVCHLGLTTGGSIGAETVLQLRDCPALVDRWAEAALKIK
                     SDAIILVHGGPVAEPADADFIMKNTRHCHGFYGASSMERLPVEVAIREQTRAFKAISR
                     SGGTDNAAGTARGSTTSR"
     gene            11112..13991
                     /locus_tag="PK812_04065"
     CDS             11112..13991
                     /locus_tag="PK812_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin domain-containing protein"
                     /protein_id="HRJ68762.1"
                     /translation="MTGQLIGTLILWLIVAVIVIAIIVYLVNWLYRRSSKEVSFVRTG
                     LFGEKVVINGGAFVLPIIHDVTPVNMNVLRMAVTRSNNEALITRDRMRVDIDAEFYVR
                     VRPQRDAVSVAAATLGRRTLQPDQLNALLSGKLVSALRSVASEMTMEEMHEKRGIYVQ
                     RVKEAAVEAFDQNGLELESVAITDLDQTDLQYFNPSNRFDAEGLTRIIEEIEEKRKLR
                     NDIEQEAMIKIRTRNLEAERQALDIERESESARLEQQREIEIKRAMQRAEVARERAQR
                     DTEAEQAQIQSREEIEKARISNERAISEARIASERDIRQKEIERTRTVEEAEIAAREF
                     VEKARISNEQAINAARIASERDIRQKEIERSKVLEGAEIAAREATEKARILQEAALNA
                     ERIAREQEVRNREIERTRAIEQEDIAAREAIETARIAQEERVRALQIARNKALDEAEI
                     AAREAIEKARIAQEIALTADRIASEEGRRLLEIQRDEKLEAATIASRELVERRRIEQE
                     RTVSTERIQRDETVRALEIARNKAIDAAEISAREATEAARIAQEKALAAERIAAELST
                     KELEIKRAASLEAEEIKRRDSVERQRIATELKLEQERIASTKTREMLNIDQKKSIEVA
                     EEERAITIAAKKVERAEADKTLRQAEIVARQEVEKIDISREQALEAARLERRRVLEQL
                     EIARAQALQEAQISSNEEVERARIASDRGLDEARVGRQRDLRKLEIERERDVESAAME
                     KAIAIYAKSLEESAAQVKAEMARAKAVEAEELVKTVKESEGAKRRKAVEVLLAQKAAD
                     ETRIAAEAEKVRRAVEAEAQKLLYEAENVLTDGARYGMFRRRLLDRIEGIIRESVKPM
                     EKIDGIKILHIDGLAGGGGGSSASRTPTDEVIESALRYRVQAPLIDEVLKEVGIEGGS
                     LAKMGGLIREASDMQRIGKDSQGAKPKDGGGAGTGGAGTGGKSKG"
     gene            13998..14435
                     /locus_tag="PK812_04070"
     CDS             13998..14435
                     /locus_tag="PK812_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRJ68763.1"
                     /translation="MARVYVSSVINAPAARVWARVRDFNGLPNWHPAIAESRIENGEP
                     ADKVGCIRAFSLRNGDRLREQLLGLSDYDMFCTYSILDSPMPLTNYVATLRLTPVTDQ
                     DRTFIEWSADFDCQPDREAELVSGIGGNVFQGGFDALKRAFGG"
     gene            14437..14889
                     /locus_tag="PK812_04075"
     CDS             14437..14889
                     /locus_tag="PK812_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRJ68764.1"
                     /translation="MPKVVRSTIIDVPVDRLWAVVRDFNAHDQYHPVVATSLIERGHP
                     VDRVGCVRRFTLRDGSELREQLLALSDLEMSYSYCLLDTPIPLFNYVAHIRLLPVTDG
                     NRSFWHWESRFTTPPGREAELARMVGDEVYVAGMEAVRQLPQLMREGH"
     gene            14891..15715
                     /locus_tag="PK812_04080"
     CDS             14891..15715
                     /locus_tag="PK812_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HRJ68765.1"
                     /translation="MPLDVRATTTMAEAAGLLASDRRTCLVAGGTLVLRDVNEGRLLD
                     GTLLRLTDPEFRRMNSAGSRVELGAGVTMAMVMANRDLAFLHPAARAVGGPAVRNMAT
                     IGGNLFAATPYGDFAVALLALDAQVLVQAGYGSARAVPIEEFLNARERGVSGLVTGVQ
                     FSRPQNGADFRFQKVTRVKPKGLAILSIAAHLPNSGARISQPRVAYGAMAPTPVRARG
                     VERALEGKALDAGSIQAARAAALEGTRPATDAISSEWYRREVLPVHLGRLLAGERH"
     gene            15726..16208
                     /locus_tag="PK812_04085"
     CDS             15726..16208
                     /locus_tag="PK812_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRJ68766.1"
                     /translation="MAKIPVQFRLNGSDRAAFVEASENLLTTLRRGLNEFAPKYGCGQ
                     GTCGACTVLVDGEPQLSCLTLSVACENRHVETASGMADGPTLHALQVAFMDHFAAQCG
                     FCTPGMLMAARALLDRNPSPTREDVVEAIAGNICRCTGYEPIISAILAAAGARSPQRA
                     "
     gene            16222..>17844
                     /locus_tag="PK812_04090"
     CDS             16222..>17844
                     /locus_tag="PK812_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRJ68767.1"
                     /translation="MLEIRKDFFANERDDNLNVIGKGVQRQDMPGHVTGRTRFFDDHA
                     FEGLLHLKVVRSPHHHARIRAIDISAAERAPGVKRILRGADVPVNKNTLLSLINFGKD
                     DEPSLAVGKVSYKGEPVVAIVAESEAAAMAARKLVRVDYEPLPAVFDVEEALQPGAPL
                     VNETYPHNYFEYHEKFDHQKLRFGDVDAAFRRADLVLEERYQTSPIEHAPTETNGSIA
                     APEHNNRFVVHSCAQGLFFSLGTAAKILDLDSNRLHFIGGTVGGGFGGKVDSLTEPLA
                     ILGAMLTGKPVRYVLDREEEMLYGSPRGAERIYIKDGLMRDGRIVARHIRSYFDAGAY
                     TRLSSYAAIKCAAHLPGPYWIPNVTSDIHVVYTNRCPSTAMRGFGITAVDFALEVQMD
                     KLAHAISMDPMEFRILNAYRDGDMKAHRRVAKNTALIECVQVAAEKSRWPISEQARRD
                     SSLQGGGQGDRGLIPATPIDQRGQIGRPETRTAAATQTLPAGTMRIPLTKQPIMEGSA
                     DARPVAPNVPRYEPARPLVVILVAHHGDAEHRA"
BASE COUNT         3143 a         5826 c         5750 g         3125 t
ORIGIN      
        1 ggcgccatca gcgatggccc ggccaccagc gcgcgtcccg cggcgtcgtg agcctgcctt
       61 tcggatcccg ccctgactcg gtcagagtcg gtccgcgccc gaccgaagcg acgggatcgc
      121 gccatttcag ccttgttcga tcaggccgga accatactct accccccttg ctgtcgcgtg
      181 ccggcgaacc ttcgcctcgg gagccttgaa ccatgctgat cgcccatgat cctgtccgcg
      241 ccttcgtgcc ctatcccgcg acggcggtcg ccggcacggc ctccggcccg ctcgcgagcc
      301 tcagcttcgg cgtcaaggac attttcgacg tcgccggtta tccgaccggc tgcggcaacc
      361 cggtgaagct ggccgaatcc ggcctgcgcg ccacacacgc accggcggtt caggccctgc
      421 ttgacgctgg cgcgagcttt ctgggcaaga cccatacgga agagcttgcc tggtcgctct
      481 acggcaccaa tgtgcatttc ggaacaccgg tcaacccggc agcgccatcg cgtgttcccg
      541 ggggctcttc ttccggatcg gccagcgcag tcgcggccgg cctctgcgac atcgcccttg
      601 gctccgatac gggtggctcg gtgcgtgctc ccgccagttt ttgcggcatc tatgggctcc
      661 ggccaagcca tggaagcgtt tctctcagtg gctgcatgcc gcttgcgccg agcttcgaca
      721 cctgcggatt cttcgcgcgg gacgccgcca ccatgctggc gacggggcgc gtgctgatcc
      781 gtgacgccca cgtcccggcg acgcgcctgc tgatcgccgc cgatctgttc gacaggctcg
      841 cgcccgccgt caccgacgcg ctggcgccca ggctcgccgc cattcacgcc cttctgggaa
      901 agggcgagac gaccagggtc tacacgcagc cgatcgaccc gctctacacg gccttccgcc
      961 acctccagag ccgtgaagtc tatgccattc atggcggctg ggtggagaag catcatcccc
     1021 cattgggccc ggccatcgca ggtcgcttcg acttcgcgcg cgccgtcacg gaccgacagg
     1081 taagcaccgc ccgggcgatg cgcatagaat tcgcggcgca catgcaggcg ctgcttgcca
     1141 acggaggcgt gcttgtcgcc ccgacagtgc cgggaccagc gccaaggctc gacgacagcg
     1201 aggacaccct cgaccgctat cgccacgagg ccatggtgtt cctccagcct gcgggccttg
     1261 ccggcctgcc gcaactcgtg ctgccggctg gaacggtgga cgatgcaccg gtcggaatct
     1321 cgctgatcgg cgcgcgcggc agcgaccttg ccctgctgga gctggcggat cggctcggct
     1381 gagccgggca gcggctcagc gaaaatgaga cgcttggata ttggccttcc gcagttccag
     1441 cgacagcttt cgtcaggcgc gtccctgggg cgcgtgtgga ggcgcgcaga gggaagtccg
     1501 gcgtggttgc ctggagcact ctcggcgtca tagggaggaa acaatgcgca agtctttgct
     1561 cggtgcggtt gctctggctg ctctcatgct gccgggagcc gcctatgccc agtccggaac
     1621 aatcaagatc ggaattcttg tggcgctcga gggcgccttt gccgtcggcg gccaggatgg
     1681 cgtccgcaat gtcgaactcg cccttaagca ggtgaacaac accattgcgg gcaagaaggt
     1741 cgagacggtc gtcgccccga ccgacacgaa gccggacacg gccgtccgca tggcccgcaa
     1801 gctgattgag caggacaagg tcgatctgat catcggcccg ctctccggct ccgaaggcat
     1861 cgccatgcgc gattatgcca agaccattcc cggcaatgtc gtgatcaatg gcgtttccgg
     1921 tgctctggag acaacctggg tggatccggc gccgaacttc taccggttca atctcgatgg
     1981 cggacaatgg ggttacggtc tcggcagcta tgcgttcaag accaagggct acaagaaggt
     2041 tgcagcgatc actgcggact attccttcgg ctacacgaat ttcatgggtt tcgcggtcga
     2101 ctactgcaag gcagggggcg acatcgttca tcgcttctgg cagcccctcg gtcaggctga
     2161 ctttgccggc atcatcgcgc agttgccgga caacgtcgat gcgatctatc tcggcctggg
     2221 cggtaccgat gcgatcaact tcctaaacca gtaccagcag gccggcgaaa agaccaagct
     2281 gatcggcggc acgatcctgg ccgatcagac cgtcctcacg gcgcgcggcc gcgccaagga
     2341 aatcctcaag ggcacccccg tctccggtcc gttcgcgacg gatgattcca gcccggcatg
     2401 gcaggcctac atgaagctgt accaggacgc ctggccggcc aatcagcgcc tgccctcccc
     2461 ctcgctcttc ggccttggct actacacagc gactctcgcg gcgatcaaag gccttgaggc
     2521 tgccggcggc gatctgtcca acggacaggc caagttcaag gaggccctga ataagctcca
     2581 gcttgacagc ccggtgggca agatcagcct caacgagaac cgtcaggcca gcggcacggt
     2641 cttcatcaat gaggtcgtgg atgggcccga cggcaacctc accaccaaga atgtggcgaa
     2701 gttcgagaac gtcacccaga ctttgggcat gacggtggcc cagttccgcg cgctcggact
     2761 gccttcgcgc accgtggtcg attgcgccaa gctgcgctca ggcggctagt cggaattccg
     2821 actttcgaaa cggggcgggc gattggccga acacggtcat tcgcccgcgc cgaacagccg
     2881 acaagtcgga tcacttctgg ccttcggggg ctcgcccgtg accctgatca gctatctttc
     2941 cagaacgcac ttcgccgaag ccgcgatcga ggatgccctt ccggaggaac tcggtcaggt
     3001 gtcacgcgcc ctgctgctcg tcgacgatga tgacgcggca aatcttgcgg cttcacgggt
     3061 ccaggacgcc ctgaccggaa tctcgctcag cgtcgtcagg gcccgttcca gagttgcacc
     3121 ggcggtgatg atagcccggc ttctggaagc cttgaccgac attcagtcaa acacactgat
     3181 tgcagtcggc ggacctgagg ccattggtct ggccaggcta tgcgccgaaa gggcgcggcg
     3241 aagcggcgct cacctcaagg ttgtcgctgt gccggctgga atcagcgact tcggtctttc
     3301 aaggcgcgtg cggtcgagcc agggccagta cctggcatgc ccccggcccg actgcattgt
     3361 cgcggatccg acggtgctca agccggtcga aagccgccgg ctggctgctt cggcgatgga
     3421 agtgctggtg cacgccgtcg aggcctatgc cagccccggc tacaacccgc cggccgacgc
     3481 catggcggcg gatgccgtgc gacggatcac gcactggctg ccccgtgcat tggcagaccc
     3541 ttcgaattcc gaagcgctgc gagagttgct ggcttccgcg ctgactgccg gtctggcgct
     3601 cgaaaaagcc tttggcggtg tcgatgcctt cgcggttccc attcatgccg agcttggttc
     3661 cggactgttg cacggcgccg ttcacggccc gctgatggcg gccatggccg agttcaatgg
     3721 cgaggctgtc ggcgaacgct acacgaccat ggcgggatat ttcgccaaca ccgatcaccg
     3781 agcgcatttc agtgcggaga tcggtgcgtt cgcgcgtaac gtcggcatgc ccgcgaccct
     3841 gagggagatc ggcgtcgacc ggtcgcaact cgggcgcctt gccgaagttg ccgccaacga
     3901 tcctgcggcg ctcgcaaatc cgcggcggct gacgtctggc gattgccgtc ggatccttga
     3961 ggcggcctgg tgatgcgggc actggcaaac attcctgggt cgaatggtcg gattgcgccg
     4021 ccaaccctat tgtcaccgcc cgcacccatt ctactattgt ataaagccgc ccaaagagcg
     4081 cggggaggaa acggatgagt ctcgcccacg cagtcgcgca tggtgcgttt ggtcgcgcat
     4141 gtctttacga actcgacagg ccgatggttc ctcacgcgca tcgcgaagga cacctgatct
     4201 tccacgtcag cggaccggcg gccacggttg tcgtcgatgg taaggtcctg ccactcgatc
     4261 cgctacgagc aaccgccatc agcccgtggc agccgcatca tttcgtgccc ataagccgtc
     4321 gggagaccac tctcacgctc gttctctaca tccggcccgg atggttcgtc gatgcaagcc
     4381 cgaccgcttt cgaaatcctg cggtttggac gatcaaccgt tgaaatgagt gatcacatcc
     4441 gccgtcttgt gctggagacg gcgcgcctgc tgggagcaca tggcggcgaa catggcatgt
     4501 tcgaggatcg cctgggcgcg ctgacacagg cgtccttcga tcagacctgg cagtggaccg
     4561 gaaacgccga tgcccgcgcg caacgtccgg cggccctccg cgacttccgc ctgcgcaggg
     4621 caatcaacat cctgagcgac aatctgagcg aaacactcga tctggccgag gtctcccgcg
     4681 cctccggcct gtcgcgcccg catttcttca agcttttccg cgagcagatg ggattgccac
     4741 cgacgatatt catcaacgcg ctgaggatgg agcgcgcgat cgagcggctg gcccgcggcg
     4801 aggaaacggt ctccgagatc gggcttgatc tgggcttcgc cacgcccgcc agtttctcgc
     4861 ggttctttat tgccaatggc gttgtgcctc ccacggccta tcggcgcagc gtcatcggac
     4921 tggcgcactg aacgatgcgg aggagacgaa actaagatca gacgcttgga taagagacaa
     4981 gacgttcggg aaacgacagt ccggctttct gtctctaggg taccggcgtc tctggcagtc
     5041 gctcacaaca ggaatctccc ggatccgtcg aaatggacga tttcatcagg cgccaccctt
     5101 tctggagcct tgtagccatt ctcgccgtgg ccgcccttct ctggctggtg ctggcgccct
     5161 ggccgcccga gctcgtggcg ctctggggac gcaagcagat ctttctcaac gcactgctcg
     5221 gcggcatcac gctgggtgcg ctgtatttcc tcgttgccgc gggtttcacg ctcattttcg
     5281 ggctgatgcg tgtcgtcaat ctggcccacg gttcgctgtt cctgatcggc ggctatctcg
     5341 gctacgagat cgcgaccgcc tcgggatcgt ggctgctggc tttcccgatc gtgttcgtca
     5401 tcgtggcggt gatcggtctg ctgatgcagc ggttcatctt ttccaagatg gaaggcgagg
     5461 aactgcgcca gacactcgtc agcatcggcc tgtcgattgt cttcgccgat ctgatgctct
     5521 ggtggtgggg tggacagtcc tattccattc ttgcgccgca gttcctgcag gggccgatgg
     5581 agctgccgat catcaacaac gtcaacgcca cgaccggagc ggtgacctgg ctgaggtatc
     5641 cggcggtgcg cgtctggatc ctggtcgcgg cgatcatcac cggcctggcc atgtggctgg
     5701 tgctgaaccg cacccagctt ggcatgctga tccgcgccgg tgtcgatgat cgggaaatgc
     5761 ttgctgcctc cggcgtgcga atccagctgg tgtttctcgc ggtgttcggg tttggcgctg
     5821 gacttgccgg cgtggccggc atcatcggag gcacgttcca gtcgctgtca ccgggcgagg
     5881 atacgcgctt cctgctcgcc agccttgtgg tggtcatcgt cggcggcatg ggatcgatcg
     5941 ccggcgcggc gcttggcgcc ttgatcatcg gagtgaccga gcagatgggt tttgtctatg
     6001 cgcccaccta ctctgtcgtg ttcacgttcc tgatcatggc tgcggttctg gcgtttcggc
     6061 cgcagggcct gttgggaacg gggcgctagc cgatgtcagt cactgacccg gcccctcgat
     6121 ttgtcacccg ctccgagcag cggatgtggt ttgagcgcat tcccgtggtc tggtggattg
     6181 tcggtcttgc cctgctcctg ctgccattgg tcgccaacaa cttctggctg ttccaggtca
     6241 tgggctggag cttcatcctt gggatgatcg gtctcagtct gatgttcctg gccggctatg
     6301 gcggcatggt cagcctgctt cagatgtcga tcgccggcat ggcaggctac atggtcgcga
     6361 ttctcggaca gtccgggaca gccacggtca gtctcggtct ggcctggtgg atcgtgctgc
     6421 cgaccgccct cctgattgcc gcgctgttcg cgacgattgc cggcgctctg tccgtgcgca
     6481 ccgaaggcat ctacaccatc atgatcacgc tcgcgattgc ggcggcgttc ttctacttca
     6541 cccggcagaa ttacacgatc ttcaacggct attccgggtt caacctcgtc cttccgccca
     6601 agcttttcgg cgtgaactgg cgcgatccga tcccgttcta ctacctgagc ctggggtgcg
     6661 cgacgctcag ttacggcgcg gtcgtctaca ttgcccgctc gccgttcggc ctggccttgc
     6721 agggcgtgcg cgacaacccc cgcaggatgg ccgcgctcgg gttcaacgtc acggcccatc
     6781 gcgtcgccgc ccatgcgctg gcgagtgtct tcgcctctgt cggcggcatc ctgctggtct
     6841 ggcagaatgc acagatcgcg ccgggaacga tcggcatatc cgctgcgatc gatgtgctcg
     6901 ttgtcgccgt ggtcggcggc atgcgacggc cgctcggccc cttcatcgga gcactgatct
     6961 acgttgtgct ccaggtgttc tcaccggaca tcctcggctt ctttggcttc gcggcggatc
     7021 ggttcaagct catcatcggc ctgggtttcc tcgcggttgt cctgttctcg cccgacggcg
     7081 tcctcggcat ctgggaccac tggcgtgcgc gcgaggcgcg gcgcaaggcc gaggcgccgg
     7141 gaggctgggc atgaacgcgc cgctgccccc cgcacagatg agcgccatcg gcgcgcgcaa
     7201 cgccagttcc ggcaacgcgc tcgaattgcg cggcataacc aagatgttcg gggcgcttgc
     7261 cgcgatttcc gacgtgacgc tgtcggtccg gccgggcgaa aggcgcgcgg tgcttggctc
     7321 gaatggcgcc ggcaagacca cgctgttcaa ttgcattacg ggcgacttcc tgcccagcgc
     7381 aggcttcatc cgcttcttcg gcgaggacgt gacgatcttc ccgccgcatg agcgaattcg
     7441 gcgcggattg cgccggacct atcagatctc gtcgctcttc actggcctga gcgtgatgga
     7501 caatgtctat ctcgcttgcc gcggggtatc ccgcaaccgc ttctcgctca tccggccccg
     7561 gcgtgacgat gcactgatgc atgcggccga gatacttgtc gaggccgtgc acctgacagc
     7621 ggaacgggac cgtccggttg gcgaactcgc ctacggacag cagcgtcagc tggagatcgc
     7681 gctcgccctt tccggcgccc cgcggttcat cctgttcgac gagccggcgg ccggattgtc
     7741 gccgaccgaa cggcgcgatc tcgtccatat cctttcctcg ctgcccgcgc atatcggcta
     7801 catcatcatc gagcacgaca tggacgtggc gctgcgcgtc gtcgagagcg tgacgatgat
     7861 gcataatggt cgaatcttca aagaagggcg cccggaggag atcgaggacg accccgaagt
     7921 gcaggaactc tatctcggag gcggccatga gtgagcgcgc gccccgggtt cctggcgcat
     7981 cccagccgat ccttcagctt cagggcctca acgtgttcta cgggcgcagc catgccttgc
     8041 agggcgtgga tctgacgctg cattccggcg tgctgtccgt cgtcgggcgg aatggcatgg
     8101 gcaagaccac gatgtgcaag accatcatgg gccttgtgaa ggccacttcc ggttcgatcc
     8161 gcttcatggg cgaggaactg ttgggccgca cgccggcgga gatcgcgcgc aagggcatag
     8221 gctatgtgcc gcagggacga cgcctttggc gttccctgac ggtggacgag cacctccggc
     8281 tgatgtccac cggaaagggc agttggacgc ctgagcgcat ctatgaggtc tttccccgcc
     8341 tcgcggaacg tcgtcgcaac ggcggcggac agctttccgg cggcgaacag cagatgcttg
     8401 cgatcagccg ggccctgctc atgaatccga aactcctgat catggacgag ccaaccgaag
     8461 gccttgcccc ggtgatcgtc gcgcacgtcg aggagacact ggtgcgcctc gccgatgagg
     8521 gcgatgtctc gatcctggtg atcgagcaga acattggcgt cgccacgcag atgtcggatc
     8581 ctgtggccat catggtcaat gggcgcatcc accaggtgat cgcctcgcag accctggctg
     8641 cggaccgcga ccttcagcag cgtctgctcg gtgtcggacg ccacagtcat gacgagaccg
     8701 acgtcgccgc aggcgatgcc gggcgcgcgc gatcagccga tcccgcccag ccggtcaacc
     8761 aggggccgat ccgcgtctac aacgccaatc caacgctgcc gacgcgctgg tcgcaggaca
     8821 ttcctgccgc acggatcgag gcggctgcgc gcacgatctc caggccaacc ctggcgacca
     8881 tagacggacg gcgcgtcggc gacatctcgc cgcttgccgc tcggacgccc caggaaccgc
     8941 atgtcattgt cgcggggacc ctcgatacca agggcgacga gctgcgctac atccgcgaca
     9001 tcatcaaggc tgcgggactt cgcacgcgcc tggtcgatct gtcgacgaac ggtcgcaacc
     9061 agggggccga tgtctcggcg caggaagtgg ccctcgcctc gcccaaggcc tccgccggca
     9121 tcgcctcggg ggaccgcggg acggccgtcg ccgcgatgac cgaggctttc cgcgcctggg
     9181 tggagcggca gcagaacgtc gcagggatca tagccgccgg cggctccggc ggaaccgcca
     9241 tcgcgacagc cggcatgcag gtgctgcctt tcggcgtgcc aaagatcatg atctcgacca
     9301 tggccgccgg gaacgtcgcc gcctatgtgg ggccgacgga catcacgatg atgcattccg
     9361 tgaccgatgt tcaggggctc aaccgcgtat cacgcgcaat actcggcaac ggcgcccatg
     9421 cgatgacagc catggcgcgc ggctggatcg agcgtcaggc ctcgcgcaac acgcccgtgg
     9481 ccggactgcg ccccggtcct gaacgcccgc tcgtcggcct gaccatgttc ggcgtgacga
     9541 caccctgcgt ccagcaggtg acaaagctcg tcgaaccgga ttgggaaccg ttggtgttcc
     9601 atgccaccgg catcggcgga cgctcgatgg agaaattgat cgattccggc ctgctgcagg
     9661 gcgtcatcga tgtctccacc acggaagtat gcgacatgat gatgggcgga ttgcttccgg
     9721 ccacggacga tcgctttggc gccgtcatcc gcacccgcct gccctatgtc ggctcggttg
     9781 gcgcgctcga tatggtcaac ttcgccgcgc ccgacaccgt tccggcacag tatcgtggcc
     9841 gccttttcta cccgcacaat ccgcagatca cgctgatgcg cacgacggtg gaggaaaacc
     9901 ggcgcatggg ccgctggatc ggcgagcggc tcaaccagat ggaaggcccg gtgcggttcc
     9961 tgatcccgga gggcggcatt tcggcactgg atgcgccggg caagccattc tgggacccgg
    10021 cggccgatga cgcgctattc agtgccatcg ccgagacgtt ccgcacaacg gcaaaccggc
    10081 agctcatccg actgccgcat cacatcaacg atcctgcctt cgccgcagcg ctggtccagc
    10141 acttccggtc gctgcaccag gtgcgcgcgc gcggatcatc gagccggacc tgagccatgc
    10201 cgagattttc ccgctccgag ctcctcgcac gctaccgcgc aatgatcgcg cggggagaac
    10261 ccatcgttgg tggcggggcg ggcaccggcc tgtcagcgaa atgcgaggag gctggcggga
    10321 tcgatctcat cgtcatctat aactccggcc ggttccgcat ggcgggtcgc ggctcgctct
    10381 ccggtctgat gccctatggc gacgccaacg cgatcgtcat ggagatggcg gccgaggtgc
    10441 ttccggtggt caggaagacg ccggtgatcg ccggcgtgtg cggcaccgat cccttccgca
    10501 tgatggatgt cttcctcgac gaggttaagc gcgtcggctt ctcaggcgtg cagaactttc
    10561 ccacggtcgg gctgatcgac ggggttttcc gcaggaacct tgaggaaacc ggcatggggt
    10621 acggccttga ggtcgacatg gtggcgctcg ccggtcgccg cgatctgctc accacgccct
    10681 acatcttttc ggaagcggat gcgatcgcga tggcgggcgc cggagcagac atactggtct
    10741 gccaccttgg gctgacgacc ggcggctcga tcggcgcgga aaccgtactc cagctcaggg
    10801 attgcccggc gttggtcgac cggtgggccg aggccgcgct caagatcaag agcgacgcga
    10861 tcatcctcgt ccatggcggc cccgtggccg agccggcgga cgccgatttc atcatgaaga
    10921 acacgcggca ttgccacggc ttctacggtg catcgtcaat ggagcgcctg ccggttgaag
    10981 tggcgatccg ggaacagacg cgcgccttca aggcaatcag ccgctccggc ggcacggaca
    11041 atgcggcggg cacagctcgc ggatcaacga catcccggta aggccgggca ggtggatggg
    11101 agaaacgcgc catgacgggg caactgatcg gaaccctcat tctgtggctt atcgtggccg
    11161 tgatcgtcat cgcgatcatc gtctatctgg taaactggct gtatcgccga tcgtccaagg
    11221 aggtttcgtt cgtccgcacc ggcctgttcg gggaaaaggt ggtgatcaac ggcggtgcct
    11281 ttgtcctgcc gatcatccac gacgtcacgc cggtgaacat gaacgtcctg aggatggcgg
    11341 tgacgcgctc gaacaacgag gcgctgatca cgcgggacag gatgcgcgtc gatatcgacg
    11401 ccgagttcta tgtccgcgtc cggccgcagc gcgacgcggt atccgtcgcc gccgccacgc
    11461 ttggccgccg cacgcttcag cctgaccagt tgaacgccct gctctccggc aagctcgtgt
    11521 cggcgctgcg ctcggtcgcg tccgaaatga ccatggagga gatgcatgag aagcgcggca
    11581 tctacgtcca gcgggtcaag gaagcggccg tcgaggcctt cgatcagaac ggactggaac
    11641 ttgagagcgt cgccatcacc gatctcgacc agaccgacct gcaatacttc aacccgtcca
    11701 accgtttcga tgcggaaggt ctgacacgca tcatcgagga gatcgaggaa aagcggaagt
    11761 tgcgcaacga catcgagcag gaggcgatga tcaagatccg cacccgcaat ctcgaagccg
    11821 aacgtcaggc gctcgacatc gagcgggaaa gcgaatccgc gcgcctcgaa cagcagcgtg
    11881 aaatcgagat caagcgcgca atgcagcgcg cggaagtggc gcgcgagcgc gcccagcggg
    11941 atacggaagc cgagcaggcg cagatccagt cgcgcgagga gatcgaaaag gcccgcatct
    12001 ccaacgagcg cgccatcagc gaggcacgca tcgcatcgga gcgcgacatc cgacagaagg
    12061 aaatcgagcg gacacgcacg gtcgaggagg cggagatcgc cgcccgcgaa tttgtggaaa
    12121 aggcgcgcat ttccaacgag caggccatca acgccgcccg catcgcctcg gagcgcgata
    12181 ttcgccaaaa ggagattgaa cgctcgaagg tgctggaagg cgccgagatc gcggcgcgcg
    12241 aggcgaccga gaaagcgcgc atcctgcagg aggctgcgct caacgcagag cgcatcgccc
    12301 gcgagcagga ggttcgcaat cgcgagatcg agcgcacaag ggccatcgag caggaggata
    12361 tcgccgcgcg cgaggccatc gagaccgcgc ggatcgcaca ggaggaacgc gtccgcgcgc
    12421 ttcagatcgc ccgcaacaag gcgctcgacg aggcggagat cgccgcacgc gaagcgatcg
    12481 agaaggcgcg catcgcgcag gagatcgcgc tgaccgccga tcgcatcgct tcggaagagg
    12541 gccgccggct tctggagatc cagcgtgacg agaagcttga ggctgcaacc atcgcttcgc
    12601 gggaactggt cgagcgcagg cgcatcgaac aggagcggac ggtctcgacc gagcgcatcc
    12661 agcgtgacga aaccgtgcgg gctctggaga tcgcccgcaa caaggccatc gacgccgcgg
    12721 agatctcggc ccgggaggca accgaggcgg cccgcatcgc ccaggaaaag gcgttggcgg
    12781 ccgaacggat cgctgcggag ctttccacca aggaactgga gatcaagcgc gccgcatccc
    12841 tggaggctga ggaaatcaag cgccgcgaca gcgtcgagcg tcagcgtatc gccacggagc
    12901 tcaagctgga gcaggagcgc atcgcgtcca ccaagacgcg cgagatgctg aatatcgacc
    12961 agaagaagtc gatcgaggtg gcggaggaag agcgcgccat caccatcgcg gccaagaagg
    13021 tcgagcgcgc cgaagccgac aagaccctgc gccaggccga gatcgttgcc aggcaggaag
    13081 tcgagaagat cgacatcagc cgcgagcagg cgctcgaagc cgcgaggctg gagcgccgcc
    13141 gcgtcctcga acagctcgag atcgcgcgtg cccaggctct gcaggaggcg cagatttcct
    13201 cgaacgaaga ggtggaacgc gcccgtatcg cctccgaccg tggccttgac gaagcgcgcg
    13261 tcggccgcca gcgcgacctg cgcaagctgg agatcgagcg cgagcgcgat gtggaaagcg
    13321 ccgcgatgga aaaggccatc gccatctacg cgaagtctct ggaggaatcg gcggcgcagg
    13381 tgaaggccga gatggcccgc gccaaggcgg tggaagcgga agagctcgtc aagaccgtga
    13441 aggagagcga aggcgccaag cgtcgcaagg ccgtggaagt cctgcttgcc caaaaggccg
    13501 cggacgaaac caggatcgcc gccgaagccg agaaggtgcg cagagcggtc gaggccgagg
    13561 cgcagaagct gctctacgag gccgagaacg tccttaccga cggcgcgcgt tacggcatgt
    13621 tccgccgccg tctgctcgat cgaatcgaag gcatcatccg cgagagcgtg aagccgatgg
    13681 agaagatcga cggcatcaag attttgcaca tcgacgggct tgccggcggc ggcggtggct
    13741 cctctgccag ccggacgcca accgatgaag ttatcgaaag cgccctgcgc taccgcgtcc
    13801 aggcgccact gatcgacgag gttctcaagg aggtcggcat cgaaggcggc agcctcgcca
    13861 agatgggagg tctcatccgc gaggcgagtg acatgcagcg catcggcaag gacagtcagg
    13921 gcgccaagcc gaaggatggc ggtggcgctg gcaccggcgg cgccggcacc ggtggcaagt
    13981 caaaggggta agcggcgatg gccagggttt acgtatcgag cgtcatcaac gctccggccg
    14041 cacgggtctg ggcccgcgtg cgcgacttca acggtctccc caattggcac ccggccatcg
    14101 ccgagagccg cattgagaac ggcgaaccgg ccgacaaggt cggctgcatc cgcgccttct
    14161 cgctccggaa cggcgaccgg ctgcgcgagc agctgctggg cctgtcggac tacgacatgt
    14221 tctgcaccta ttcgatcctc gacagcccga tgccgctgac caactacgtc gcgacgctgc
    14281 gcctgacgcc agtcaccgac caggaccgaa cgttcattga gtggtcggct gacttcgatt
    14341 gtcagcccga cagggaggcg gaactggttt ccggcatagg cggaaacgtc ttccagggcg
    14401 gcttcgacgc tctcaagcgg gccttcgggg gctgatatgc cgaaggtcgt tcgcagcacc
    14461 attatcgacg tgcccgtcga ccggctctgg gccgtggtgc gcgacttcaa cgcgcacgac
    14521 cagtaccatc ccgttgtcgc caccagcctg atcgagcgcg gtcatcccgt cgacagggtc
    14581 ggctgcgtgc ggcgcttcac cctgcgcgat ggcagcgaac tccgggagca gctgctggca
    14641 ctctcggacc tcgagatgag ctacagctat tgcctgctcg atacaccgat cccgctcttc
    14701 aactatgtcg cgcacatacg gttgctgccc gtcaccgacg gcaacaggag cttctggcat
    14761 tgggaaagcc ggttcaccac gccaccgggg cgggaagcgg aactcgcgcg catggttggc
    14821 gatgaagtct atgtcgccgg catggaagcg gtgcgtcagc tcccgcagct gatgagggag
    14881 ggtcactgag atgccgctcg atgtcagagc cacgacaacg atggccgaag cggccggtct
    14941 gcttgcctca gaccggcgca catgtctggt cgccggcggc acgctggtcc tgcgcgacgt
    15001 caacgaggga cgtctgctcg atggtacgct tctgcgcctc accgaccccg aattccggcg
    15061 gatgaacagc gccggatcgc gggttgaact gggggccggc gtgacgatgg cgatggtcat
    15121 ggcgaaccgc gacctcgcct tcctgcatcc ggcggcccgc gcggtcggtg gcccggcggt
    15181 tcggaacatg gcgacgatcg gcggcaatct tttcgccgca acgccctatg gcgacttcgc
    15241 ggtggcgctg ctcgcgctcg atgcccaggt gctggttcag gccggttatg gctcggcgcg
    15301 agccgtgccg atcgaggaat tcctcaacgc gcgggagcgc ggggtatccg ggcttgtcac
    15361 cggcgtgcag ttctcacgcc cgcagaacgg cgccgatttt cgcttccaga aggtgacgcg
    15421 cgtgaagccc aaggggctcg cgatcctgtc catcgcagca cacctgccca attcaggtgc
    15481 gcgcatcagc caaccgcgcg tcgcctatgg tgcgatggcg ccgacgccgg tccgggcgcg
    15541 cggcgtcgaa cgcgcgctgg aaggcaaggc gctcgatgcg ggaagcatcc aggccgccag
    15601 ggcggcggcg ctggaaggaa cgcgccccgc gacggatgcg atctcctcgg aatggtatcg
    15661 acgcgaggtt ctgccggtcc atctcgggcg acttctggcc ggcgaaaggc actgaggagc
    15721 atgtgatggc caagattcca gtgcagtttc gcctcaacgg ttctgatcgg gcagcctttg
    15781 tcgaggcatc ggaaaatctc ctgaccaccc tgcggcgcgg actcaatgag ttcgccccga
    15841 agtatggctg cggccagggc acgtgcggag cctgcacggt gctggtcgac ggcgaaccgc
    15901 agctctcctg cctcaccctt tccgttgcct gcgagaaccg ccacgtcgag accgcctcgg
    15961 gcatggcgga cggccccacc ctgcatgcgt tgcaggtggc cttcatggat catttcgccg
    16021 cgcagtgcgg attttgcacg cccggcatgc tgatggcggc gcgtgcgctg ctcgatcgca
    16081 atccatcgcc gacacgcgag gatgtggtcg aggccatcgc cggcaacatc tgccgctgca
    16141 ccggctacga gccgatcatc agcgccattc tcgcggccgc cggcgcgcgc agcccgcaac
    16201 gcgcctgaag gaggggacgt tatgctcgaa atccgcaagg acttcttcgc caacgagcgc
    16261 gacgacaacc tcaacgtcat cggcaagggc gtccagcgac aggacatgcc cggccatgtg
    16321 accggacgga ctcgcttttt cgacgatcac gccttcgagg ggctcctgca tctcaaggtc
    16381 gtgcgctcgc ctcaccatca tgcgcgcatc cgcgcgatcg acatcagcgc ggccgagcgc
    16441 gcgccaggcg tcaagcgcat cctgcgcgga gcggatgttc cggtaaacaa gaacacgctg
    16501 ctgagcctca tcaatttcgg caaggatgac gaaccgtccc tcgccgtcgg caaggtgagc
    16561 tacaagggcg aaccggtggt cgccatcgtc gccgagagcg aggcggcggc catggccgca
    16621 cgcaagctcg tgcgcgtgga ctacgagccg ctgcccgccg tgttcgacgt cgaggaggcg
    16681 ctgcaacccg gagcgccgct cgtcaacgag acctatccac acaactattt cgaataccac
    16741 gagaaattcg atcaccagaa gctgcgcttc ggcgatgtgg acgcggcctt ccgcagggcc
    16801 gacctcgtcc tggaagagcg ctaccagacc tctcccatcg aacacgcgcc gaccgagacg
    16861 aacggctcca tcgcggcgcc cgaacacaac aaccgctttg tcgtgcattc ctgcgcgcag
    16921 ggactgttct tctcacttgg aacagcggcg aagatcctcg atctcgattc caaccgtctg
    16981 cacttcattg gcgggaccgt cggcggcggt ttcggtggca aggtggattc cctcaccgag
    17041 ccgctcgcca tcctcggcgc catgctcacg ggcaagcccg tgcgctacgt gctcgatcgc
    17101 gaggaggaga tgctctacgg ctcgccgcga ggcgccgaga ggatctacat caaggacggg
    17161 ctgatgcgcg atggtcgtat cgtcgcccgt catatccgat cctacttcga cgccggcgct
    17221 tatacgcgcc tgtcctccta tgccgctatc aagtgcgccg cgcacttgcc cggaccctat
    17281 tggattccca acgttacgtc ggacatccat gtggtctaca ccaatcgttg cccgtcgacc
    17341 gcgatgcgcg ggttcggcat caccgcggtg gatttcgcgc tcgaagtgca gatggacaag
    17401 ctcgcccatg ccatcagcat ggacccgatg gagttccgca tcctcaacgc ttaccgcgac
    17461 ggggacatga aggcgcatcg gcgcgtggcg aagaatacag ccttgatcga gtgcgtgcag
    17521 gtcgcggccg aaaagtcccg ctggccaatc tccgaacagg cccggcgcga ttcctcgctg
    17581 cagggcggtg gccagggcga tcggggcctg atccccgcca cgccgatcga ccagcgtgga
    17641 caaatcggca ggcccgagac ccgcacggcg gccgcgaccc agacattgcc cgccggcacg
    17701 atgcgcatcc cgcttacgaa gcagccgatc atggaaggca gcgccgatgc gcggccggtg
    17761 gctcccaacg tcccccgcta cgaaccggcc cgcccactcg tcgtcatcct cgtcgcccat
    17821 catggcgatg ccgagcatcg cgct
//
LOCUS       DAOMXN010000027        17780 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_1808_length_17780_cov_11.932130, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000027 DAOMXN010000000
VERSION     DAOMXN010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 17780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17780
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1808_length_17780_cov_11.932130"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="PK812_04095"
     CDS             <1..581
                     /locus_tag="PK812_04095"
                     /inference="COORDINATES: protein motif:HMM:NF018017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ELM1/GtrOC1 family putative glycosyltransferase"
                     /protein_id="HRJ68768.1"
                     /translation="ISPERLAAARAAPPYALDRLTSPRVALLIGGDSQHFRFTDDDSA
                     RLIQRVQALAGQGASLMATVSRRTPERLATGLRPIVQEAKGFFWDGTGENPYVALLAL
                     ADAVLVTADSVNMVGEAAATGKPLMVFTPSGGTPKITKFLRGLDAAGALVPVDGTLSL
                     PYEAIDSTPAIAVDLARRYKAHRTPWVIGDER"
     gene            681..1646
                     /gene="trxB"
                     /locus_tag="PK812_04100"
     CDS             681..1646
                     /gene="trxB"
                     /locus_tag="PK812_04100"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HRJ68769.1"
                     /translation="MTGPRHAKVIIIGSGPAGYTAAIYASRALLKPLLISGFEPGGQL
                     MITTDVENYPGFADVIQGPWLMEQMRLQAEHVGTEMISDHVTAVDFSRRPFTLTCDSG
                     ASYTCDALIIATGAKAKWLEIPSETTFKGFGVSACATCDGFFYRNKDVVVVGGGNTAV
                     EEALYLANLASSVTLVHRRDSLRSEKILQDRLLKHPKVRVIWNSAIDEICGTDNPLSV
                     THVRLKNVKTGATSEVRTHGVFIAIGHKPASELFVGKLDMRPSGYLITVPGSTQTNVP
                     GVFAAGDVADEQYRQAVTAAGLGCMAALEAERWLQAQGDARQAAE"
     gene            1761..2651
                     /locus_tag="PK812_04105"
     CDS             1761..2651
                     /locus_tag="PK812_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRJ68770.1"
                     /translation="MDWDKVRIFHAAAEAGSLTKAGDVLGLSQSAVSRQVSALEAELK
                     VPLFHRHARGLVLTEQGELLFRTARELVTKLEQTQAQLMDSRQKPDGVLKVTANVGLG
                     AHWLTPRLDEFIDKFPDIQLQIILTDDELDLAMREADVALRLREPVQGDLIRRKLFTV
                     HLHVWASTEYLKRHGQPRSPQDLDRHRLLAFGGDIPSFLKDINWHLHAGRDPKEPREP
                     ILTANNITSLVKAVQGGMGIAVMPDYLVAPGSGLVQVLTSESMPELECYLVYAETLKG
                     VTRVQVFRDFMVAKAQRWAF"
     gene            complement(2990..>3630)
                     /gene="msrP"
                     /locus_tag="PK812_04110"
     CDS             complement(2990..>3630)
                     /gene="msrP"
                     /locus_tag="PK812_04110"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HRJ68771.1"
                     /translation="AIDGMVEKPMEVGIDDLIKAMPLEERVYRMRCVEAWGMTVPWTG
                     FPMKALLDYARPLSGAKFVRMETFLDPKIAPGQRSAGYPWPYVEGLTMAEAANELTML
                     VTGVYGKPAAKQFGAPIRLIVPWKYGFKGVKSIRRFTFTDKRPVGLWEALQPSEYGFW
                     ANVNPAVAHPRWSQATEEVLGQGKRVPTLIWNGYGEFVADLYAGQEKERLFM"
     assembly_gap    3631..3730
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4015..4488)
                     /gene="greA"
                     /locus_tag="PK812_04115"
     CDS             complement(4015..4488)
                     /gene="greA"
                     /locus_tag="PK812_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HRJ68772.1"
                     /translation="MERVPLTPKGFAALETELKWRQQEERPRIILAIQEARAHGDLSE
                     NAEYSSAKEAQSHNEGRIAELESLISRAEVIDTSKLSGDTVKFGATVTLVDEDTEQKK
                     VYMIVGESEADVKAGKISVSSPIARALIGKKTGASVEVNTPGGSKSYEIDRIDFK"
     gene            complement(4574..7930)
                     /gene="carB"
                     /locus_tag="PK812_04120"
     CDS             complement(4574..7930)
                     /gene="carB"
                     /locus_tag="PK812_04120"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019905202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HRJ68773.1"
                     /translation="MPKRTDIHTILIIGAGPIVIGQACEFDYSGTQAVKALKAEGYRI
                     VLVNSNPATIMTDPELADATYVEPITAEIVAKIIEKERGDRAKGFALLPTMGGQTALN
                     CALSLNKKTLDDGRGGRISVLEKFGVEMIGATAHAIDKAEDRELFREAMTKIGLETPR
                     SHHVKTLAKALEALDDIGLPAIIRPSFTMGGTGGGIAYNKAEFIDIVERGIDASPTSE
                     VLIEESVLGWKEYEMEVVRDRRDNCIIVCSIENIDPMGVHTGDSITVAPALTLTDKEY
                     QRMRDASLAVLREIGVETGGSNVQFAVDPATGRMIVIEMNPRVSRSSALASKATGFPI
                     AKVAAKLAVGYTLDEIANDITGGVTPASFEPTIDYVVTKIPRFAFEKFPGADPVLTTS
                     MKSVGEAMAIGRTFHESLQKALRSLETGLTGLDEIEIEGLGKGDDRNAIRKAIGTPTP
                     DRLLQVGQALRLGFSVDEVHQSCKIDPWFLERLKEIVDLEAKVKRLGLPDTAQAFRTV
                     KAMGFSDKRLATLTGKREEEVRAARHALAVRPVYKRIDTCAAEFASPTAYMYSTYEAP
                     FAGAPADEARPSDRKKAVILGGGPNRIGQGIEFDYCCCHACYALREVGYETIMVNCNP
                     ETVSTDYDTSDRLYFEPLTEEDVLEIMAKEQENGQLMGAIVQFGGQTPLKLAEALEKA
                     EIPILGTSPDAIDLAEDRDRFKRLLDKLKLRQPQNGIAYSVEKARDEAEKLGFPFVVR
                     PSYVLGGRAMAIIQDQEAFDDYLLGTLPSLIPSDIKARYPNDKTGQINTVLGRNPLLF
                     DRYLTDAIEVDVDCLSDGKDAFIAGIMEHIEEAGIHSGDSACSLPPRSLSPELLAELE
                     RQTRDMALALGVGGLMNVQYAIKDGVIYVLEVNPRASRTVPFVAKVIGKPIAKIAARI
                     MAGEKLADFRLKAEPIAHVGVKEAVFPFARFPGVDTVLGPEMRSTGEIIGLDSSFGVA
                     FAKSQLGGGTKVPKSGTVFASLRDDDKPRILDSLRVLVSLGFRIIATGGTQRYLQSNG
                     IACEPVKKVLEGRPNIVDEIKNGRVQLVFNTTDGAAALKDSKSLRRAALLHKVPYYTT
                     LAGAVAAAEGIRAYLEGDLKVRALQEYFAA"
     gene            complement(8095..8547)
                     /locus_tag="PK812_04125"
     CDS             complement(8095..8547)
                     /locus_tag="PK812_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HRJ68774.1"
                     /translation="MRETFTEAMKDAMKAGDKARLGAVRLIIAALKEKDIEARGAGRE
                     KASDEEILGVLQKMIKQRQESADVYEKNGRPELAAGERGEIAVISSFLPAQMSEEEVK
                     AAIAAAIAETGAAGMKDMGKVVGVLRGKFAGRMDFGKASGLVKAALSG"
     gene            8818..10014
                     /gene="carA"
                     /locus_tag="PK812_04130"
     CDS             8818..10014
                     /gene="carA"
                     /locus_tag="PK812_04130"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HRJ68775.1"
                     /translation="MATAQAQNADSWAETKATAMLVLADGTVIEGVGAGAEGIAEGEV
                     CFNTAMTGYQEILTDPSYSGQIITFTFPHIGNVGVNEEDIETVNMAASSGVRGAVLQS
                     PITEPSNYRATKHFDAWLKAHGIVVITGVDTRALTALIRDKGMQNAVVAHAPDGKFDV
                     PALKAKAKALPSMTGLDLVPQVAATQRFTWDETTWAWDRGYGRQDKPAFHVVALDYGV
                     KRNILRLLADRGCKVTVMPPTASADEVLAQKPDGVFLANGPGDPAATGEYSVPTIKAL
                     LDRRIPTFGICLGHQMMALAIGAKTLKMAHGHHGANHPVKEHATQKVEIVSMNHGFAV
                     DPATLPNNAEETHVSLFDGSNCGLKLTDRPAFSVQHHPEASPGPQDSHYLFDRFVEMM
                     KANKAA"
     gene            10037..10774
                     /locus_tag="PK812_04135"
     CDS             10037..10774
                     /locus_tag="PK812_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRJ68776.1"
                     /translation="MRLAVLADIHGNIRALEAVIAALRHETPDRIVNLGDCFSGPLHA
                     AAVADRLIELDWLTVRGNHDRWLLETPPQDMGPSDRHAFAQLDTKEEDWLRDLPATAR
                     LDGDVLLCHGTPASDTTYLTETLRGDSVREATPDEVAARLGGEAAALVLCGHTHIPRL
                     IHRPGGGLVLNPGSVGLPAYTDDDPWHFVETGTPHARYALATRASGGWSVEFRRVVYD
                     WEAAARDAAKAGRPDWAHALRTGLAMK"
     gene            complement(10833..11582)
                     /locus_tag="PK812_04140"
     CDS             complement(10833..11582)
                     /locus_tag="PK812_04140"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HRJ68777.1"
                     /translation="MGFETLKNWYAEEKAITISEDVALIARYNSKGLTAPGQTVNTTG
                     TPGNDWLIGTNGGETIDGRGGDDYIFGGGGHDSLVGGEDLDSGSDTIVGGAGNDTLRG
                     VWGDDYLYGDIGNDSLLGGMGNDVVYGGENDDQVFGNDGDDYVIGDAGDDQLWGGYGN
                     DTLTGGEGADRFYFNELGEIFGNEHDVVTDFTAGDTLVITHSILSRSTVALDPTGFGV
                     TLYVANEGGAIHAITFENVDVRTLFANMLVT"
     gene            complement(11722..12117)
                     /locus_tag="PK812_04145"
     CDS             complement(11722..12117)
                     /locus_tag="PK812_04145"
                     /inference="COORDINATES: protein motif:HMM:NF016854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HRJ68778.1"
                     /translation="MHESGRPAADRKKPRRTKADPTTRAAEASLAQLIREALAPRILP
                     EKKMFGGTCFLLNGNMLCGTYKGDVIFRVGVDAHPDALREKGASAFVMQGKELTGWIA
                     VSGAVLQSPEEARIWIGRAMLFVGSLASK"
     gene            12192..12521
                     /locus_tag="PK812_04150"
     CDS             12192..12521
                     /locus_tag="PK812_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010684817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRJ68779.1"
                     /translation="MTRTLPILAVLLLCAAPAFAQTTKGQIEAGRSLAREHCAACHAI
                     GKADRSKRRGAPAFRTIGRTYDVDNLQEALAEGISVGHKGVDMPEFQFSPDRVDALIA
                     YFRSLRR"
     gene            12594..13298
                     /locus_tag="PK812_04155"
     CDS             12594..13298
                     /locus_tag="PK812_04155"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRJ68780.1"
                     /translation="MSWIEFWNQDTSIYVSARHKALHYRQVARDIAALIDSPDAVVLD
                     YGSGEATSADIVAARCAALDLCDAAPNTRLKMAEQFAGIANIRVLSTEDVQFGEDAHY
                     DLIVANSLLQYLDEPTLDGLLDLWARRLKPGGRLILADVIPPGVSPVTDALALLRFAF
                     SGGFFMAAVLGLARTFFSDYRKLRGELGISMHAESALLTRLRNRGFSARRMPDNIGHN
                     PARMTFEAIRPGEQGR"
     gene            complement(13306..13686)
                     /locus_tag="PK812_04160"
     CDS             complement(13306..13686)
                     /locus_tag="PK812_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HRJ68781.1"
                     /translation="MKRLLWFSLGWISLGLGLLGAFLPILPTTPFMLLSAAAFAKSSP
                     RLHRWIVEHPTFGPPVRDWHAHGAISRRAKLMATAAMGAVLLLSLLLRLRWEVLAVQA
                     LCLVGAGAFILSRPDSPADARDDK"
     gene            complement(13683..14069)
                     /locus_tag="PK812_04165"
     CDS             complement(13683..14069)
                     /locus_tag="PK812_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015930935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HRJ68782.1"
                     /translation="MLALLRQFAAFFGVGLVAALVHYSTLVIGVEMVRLKPVPATLAG
                     YVLGGVVSYALNRHHTYASDRPHSEAGWRFAAVAGVGFLLTFGFMHLFVERMGLPYLP
                     MQIVTTGIVLVWSFVAHKIWTFGDTR"
     gene            complement(14080..14298)
                     /locus_tag="PK812_04170"
     CDS             complement(14080..14298)
                     /locus_tag="PK812_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68783.1"
                     /translation="MRSDVPPRESIFGSLKKIWAQADAIRRNAAKRGTHPADCGCPEH
                     RLSYEPRTKQQVDIANRKPELAKPGDSQ"
     gene            complement(14378..15475)
                     /locus_tag="PK812_04175"
     CDS             complement(14378..15475)
                     /locus_tag="PK812_04175"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HRJ68784.1"
                     /translation="MRVFHAPRFVPLNDGTITVGTDGADSIDRRPIAAGEWIIALGGN
                     DTILAGSGDDWIFAGGGNDEIRAGRGDDLVLAGDGNDWVTGEAGEDVLHGDAGNDTIW
                     GGQDNDILHGGTGDDQMFGEHGNDSLYGGTGNDTLSGGEGDDYLTGDAGRNRIYGGQG
                     NDTLVAEFGSDLLEGGDGNDLLKVNWGSHTLLGGTGRDMLDFSDWQGGPLEISLRAAV
                     YLSENGNGFVDGVEDVTGSIFADAIQGNSGANLLSGGGGSDFLMGEGGNDTLIGGEGN
                     DILHGGAGFNILQGGTGSDWFIVSDGPRKGLDVILDYSFRQGDVIDVQGNVADYRAIS
                     DQQGGSFLLDAGGNRVVHLIGHDPALAMILV"
     gene            complement(15638..16483)
                     /locus_tag="PK812_04180"
     CDS             complement(15638..16483)
                     /locus_tag="PK812_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HRJ68785.1"
                     /translation="MVGLVRPIPLPNFLLGTAGNDVIDKGRSRLDWNISSGDGNDAIY
                     AGSGRDVIDAGDGDDYVAAGDGNDRVLGGGGQDTIFGGAHNDTIDGGSGSDLLFGGTG
                     DDLLIGGTGTNFLYGEEGNDTYQASGANLEHTGFGIWLDRGFAQSRGVTDYLHDIENA
                     TGSQWSDLIVGNGGDNILRGAGGYDTLEGGGGRDTLDGGALDDVLTGGEGSDRFIAAG
                     LAAGGFDVVTDYHYHEGDVIDVAGDSASYRAIQSGADVMILDVAGNAIMQVLNVTLAN
                     GIDIL"
     gene            16637..17434
                     /locus_tag="PK812_04185"
     CDS             16637..17434
                     /locus_tag="PK812_04185"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HRJ68786.1"
                     /translation="MPDTTLILRQHALRSIIGELGDLALAYFRNRDSLGVTLKGAQDW
                     LTEADGAVERLFRQRIAEAFPGDGVFGEEQGGTAGDAAAENLWIIDPIDGTANFATGD
                     RQWCVSVGFLHRGVPELGAVLAPAQEELFLARRGGGATLNGQPIRANPITDLTRASVE
                     IGWSKRRPLALYIDAVRATMEAGAGVKRSASGALGVVHTAMGRVDAYAEAHINSWDVA
                     AALVIAREAGCYANDFFRGDAIVKGNPILVAAPGIAEAVIRTTRFLD"
     gene            17499..>17780
                     /locus_tag="PK812_04190"
     CDS             17499..>17780
                     /locus_tag="PK812_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HRJ68787.1"
                     /translation="MSIEIDLLQRLGVAVAIGAVVGVERHWRERDAGEGRRTAGLRTY
                     SLTGLLGGCAGLLEKQLAGHDGLVIGLMFLGFAVVFAQFNLREAAEERNF"
BASE COUNT         2980 a         5653 c         5762 g         3285 t
ORIGIN      
        1 ggatttcgcc ggagcggctt gccgccgcgc gcgccgcgcc gccctatgcg ctggatcgcc
       61 tgacgagccc gcgcgtggcg ttgctgattg gcggagacag ccagcatttc cgcttcacgg
      121 acgacgatag cgcgcggctc attcagcgcg ttcaggcgtt ggccggacag ggcgcgtcgc
      181 tgatggcgac ggtgtcacgc cggacgccgg agcgactggc gacaggcctg cgtcccatcg
      241 tgcaggaagc gaagggcttt ttctgggacg gcaccggcga gaacccctat gtggcgttgc
      301 tggcgctggc cgacgccgtc ctggtcacgg cggattccgt caacatggtc ggcgaggcgg
      361 cggccaccgg caagccgctg atggtgttca cacccagtgg cggcacgccg aagatcacga
      421 aatttttgcg tggacttgat gccgcaggcg cgctcgtgcc ggtcgacggg acgctttcac
      481 ttccctacga agcgatagac tcgacgcctg ccatcgccgt ggacctcgca cggcgctaca
      541 aggcgcacag gacaccatgg gtcatcggag atgagcgatg accactcttg agttttcctt
      601 gttttacaag gattacggaa aactcaagat aggaaccaag ggtcaatttt catgaccctt
      661 ggcatgacag gacgaacgcc atgaccggac ctcgccacgc caaagtgatc atcatcggtt
      721 ccggccctgc cggctacacc gccgcgatct atgcctcgcg cgcgctcctg aagccgctgc
      781 tgatctccgg tttcgaaccg ggaggccaac tcatgatcac cacggatgtg gagaactatc
      841 ccggcttcgc cgacgtcatc caggggccct ggctcatgga gcagatgcgg ctccaggccg
      901 aacacgtggg cacggagatg atctccgatc atgtcacggc ggtggatttc tcgcgccgtc
      961 ccttcacgct gacctgcgat tccggtgcca gctacacctg cgatgccttg atcatcgcga
     1021 ccggcgcgaa agcgaagtgg ctggagattc cctccgagac gaccttcaag gggttcgggg
     1081 tctcggcctg tgccacctgc gacgggttct tctaccgcaa caaggatgtg gtggtggtcg
     1141 gcggcggcaa cacggccgtt gaagaggcgc tctatcttgc caatctcgct tcctcggtga
     1201 cgctggttca ccgtcgcgac agtctgcgct cggaaaagat actgcaggat cggctgctga
     1261 agcatccgaa agtccgggtg atctggaatt cggcgatcga cgaaatctgc ggcaccgaca
     1321 acccgttgtc tgtcacgcat gtccggctga agaacgtcaa gacgggcgcg accagcgagg
     1381 tcaggacgca tggcgtgttt atcgccatcg gccacaagcc ggcgtccgag ctgttcgtcg
     1441 gaaagctcga catgcggccc agtggctacc tgatcacggt gccgggttcg acccagacca
     1501 acgtgccggg cgtgtttgcc gccggcgacg tggcggatga gcagtatcgg caggccgtga
     1561 cggcggccgg gcttggctgc atggcggcgc tggaggccga gcgctggctg caggcccagg
     1621 gcgatgccag gcaggccgcg gagtagttct gcgcgcgtca tctttccgct tgcctttttc
     1681 tgcatggatt gcgaggcttc catgcagaaa cgggagggtg gcgcgagggg ttgggaggcc
     1741 ccggacgctg cataaaaaac atggactggg acaaggtccg cattttccat gcggcggccg
     1801 aggcaggcag tctgaccaag gcaggcgatg tgttggggtt gagccagtcg gcggtcagcc
     1861 ggcaggtcag cgcgctggag gcggaactca aggtaccgtt gtttcatcgc catgcgcgcg
     1921 ggctcgttct caccgagcag ggcgaattgc tgttccgcac ggcgcgggaa ctggtcacca
     1981 agctggagca gacccaggcc cagctcatgg acagccgtca aaagccggac ggcgtgctca
     2041 aggtgacggc caatgtcgga cttggcgcgc actggctgac gccgcgtctc gacgagttca
     2101 tcgacaagtt tccggatatc cagcttcaga tcatcctgac cgacgatgaa ctcgaccttg
     2161 ccatgcgcga ggcggatgtg gcgctgcgtc tgcgcgagcc ggtgcagggc gatctgatcc
     2221 ggcgcaagct gttcaccgtg cacctccatg tctgggcctc gacggaatac ctgaagcggc
     2281 atggacagcc gcgcagcccg caggatctcg accggcaccg gctgctcgcc ttcggcgggg
     2341 acatcccgtc cttcctcaag gacatcaact ggcatctgca tgccggtcgc gacccgaagg
     2401 agccgcgcga gccgatcctg accgccaaca acatcacctc gctggtgaag gccgttcagg
     2461 gcggcatggg aatcgccgtc atgcccgatt atctggtggc gcccggctcg gggctcgtgc
     2521 aggtgctcac gtcggaatcc atgccggaac tggagtgcta cctggtttat gcggaaacgc
     2581 tcaaaggcgt cacgcgcgtg caggttttcc gggatttcat ggtcgcaaag gcgcaacgct
     2641 gggcattttg aggctaatcc ggcccgtcgg atacggacgc aaggtcaggt attagccatg
     2701 tctaatgcgc atggcggcta taaccttttg gtaattgtgc gtcgcagcaa atcatcccat
     2761 atgagggacg tccgctgcga gagccgcaca tctcctcccg gtgcagtgcc ccggcggaca
     2821 cagagccaac cgatctcctc ccggtcggcg gctcttaagc cggatgcctc gtcgtgtttg
     2881 tgacgaggcg tccggcctta ttttttcaag ctgccctcgg agccgtgcgt cccttcccgc
     2941 gcgcgccgga ttcccgttcc gtccggtgac gccggtgtgt cgccgaccgt tacatgaaca
     3001 ggcgctcctt ttcctggccg gcataaaggt cggcgacgaa ttcgccgtag ccgttccaga
     3061 tcagggtggg cacgcgcttg ccctggccaa ggacttcctc ggtggcctgg ctccagcggg
     3121 gatgcgccac tgcggggttc acattggccc agaaaccgta ctcggaaggt tgaagcgcct
     3181 cccacaggcc aacggggcgc ttgtcggtga acgtgaaacg gcggatcgat ttcacgccct
     3241 tgaagccgta tttccaggga acgatcagcc ggattggcgc gccgaactgc ttcgcggccg
     3301 gtttgccgta gacgccggta accagcatgg tcaattcgtt cgcggcctcc gccatggtca
     3361 atccctcgac atagggccag gggtagccgg cggaccgttg gcccggagcg atcttgggat
     3421 cgagaaacgt ttccatccgc acgaacttgg cgcctgagag cggccgggcg taatccagga
     3481 gcgccttcat cggaaagccg gtccagggca cggtcatgcc ccacgcttcg acgcagcgca
     3541 tgcggtaaac gcgctcttcc agcggcatgg ccttgatgag gtcatcgatc ccgacttcca
     3601 tgggtttctc gaccatgcca tcgatggcga nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn ccgccggtgg tcaggaaggc gcggcgattg cagaagacgt gctcgggcgt
     3781 tgcctgcgat tcggcgattt cccagccgcg tcgacgtttg atcagcattg ctttcccctc
     3841 actgtcactg tcacgtagga gggcacgcaa tcaccggcaa gtcacgtgat ggggaggacg
     3901 cctcgaagcg cctcgttgcc gcagggcacg ctttcgcgag cgaaacatcg cgatggaatg
     3961 atccggccgc cgacggggcg ccgcaggctt gacggggcgc cgcaggcttc cagcctattt
     4021 gaaatcgatc ctgtcgattt cgtagctctt ggaaccgcct ggcgtgttca cctcgacgct
     4081 ggcgcccgtc ttcttgccga tcagcgcgcg ggcgatgggc gatgagacgg aaatcttgcc
     4141 ggccttcacg tcggcctcgg attcgccgac gatcatgtag accttcttct gctcggtgtc
     4201 ctcgtcgacc agggtcactg tcgcgccaaa cttgacggtg tcgccggaaa gcttggacgt
     4261 atcgatgact tccgcacggg aaatcaggct ttccagttcg gcgatgcggc cttcgttatg
     4321 cgactgggct tccttcgcgg aggaatactc cgcgttctcg gacaggtcgc cgtgggcgcg
     4381 cgcttcctga atggcgagga tgatgcgcgg acgctcctcc tgctggcgcc atttcagttc
     4441 ggtctccagc gcggcgaatc ccttcggggt aagcggcaca cgttccatgg tcgatccaat
     4501 ccaaacaaaa ccggtctcgg ggcggcggcc ggccgcgcct gatcccgtcg tgaagcttga
     4561 gcgctcgccg cggtcaggcg gcgaaatact cctgcaaggc gcgcaccttg aggtccccct
     4621 ccagataggc gcggatgcct tccgcggctg cgaccgcacc cgccaaggtg gtgtaatatg
     4681 gcaccttgtg caagagggcg gcccgccgca gcgatttcga atccttcagc gcggcggcgc
     4741 cgtcggtggt gttgaagacg agctggacgc gaccgttctt gatctcgtca acgatgttcg
     4801 gccggccttc cagcaccttc ttgaccggct cgcaggcgat gccgttgctc tgcaggtagc
     4861 gctgcgtgcc gccggtcgcg atgatcctga agccaaggct tacaagcacg cgcagcgagt
     4921 ccaggatgcg cggcttgtcg tcgtcccgca gcgaggcgaa gaccgtgccg gacttcggca
     4981 ccttggtgcc gccgccgagc tggctcttgg cgaaggcgac tccaaaggag ctgtcgagac
     5041 cgatgatttc gccggtcgag cgcatctcgg ggcccagcac cgtgtcgacg cccgggaaac
     5101 gggcaaacgg gaacaccgcc tccttgacgc cgacatgggc gatcggctcg gctttcagcc
     5161 tgaaatcggc gagtttctcg ccggccatga tgcgcgcggc gatcttggcg atcggcttgc
     5221 cgatgacctt cgcgacgaaa ggcacggtgc gcgacgcgcg cggattgact tccaggacgt
     5281 agatgacgcc gtccttgatg gcatattgca cgttcatcag accgcccacc cccagcgcca
     5341 gcgccatgtc gcgggtctgg cgttcgagtt cggccagcag ttccggcgaa agcgagcgcg
     5401 ggggcaggga gcaggcggaa tctccggagt ggatccccgc ttcctcgata tgctccatga
     5461 tgccggcgat gaaggcgtcc ttgccgtcgg agagacaatc gacatcgacc tcgatggcgt
     5521 cggtcaggta gcgatcgaac agcagcggat tgcgcccgag caccgtattg atctgccctg
     5581 tcttgtcgtt gggatagcgc gccttgatgt ccgacgggat caggctcggc agggtgccga
     5641 gcaggtagtc atcgaaggct tcctggtcct ggatgatggc catggcgcgg ccaccgagca
     5701 cgtaggacgg gcgcaccacg aaggggaagc caagcttctc cgcctcgtcg cgggcttttt
     5761 ccaccgagta ggcgatgcca ttctgcggct ggcgcagctt cagcttgtcg agcaggcgct
     5821 tgaagcggtc gcggtcctcg gccaggtcga tcgcgtccgg cgaggtgccg aggatcggga
     5881 tctcggcctt ctccagcgcc tcggcaagct tcagcggcgt ctggccgccg aactgcacga
     5941 tggcgcccat gagctggccg ttctcctgtt ccttggccat gatctccagg acgtcctcct
     6001 cggtcagcgg ctcgaaatac agccggtccg aggtgtcgta gtcggtcgaa accgtctccg
     6061 gattgcagtt gaccatgatg gtctcgtagc cgacctcgcg cagcgcgtag caggcgtggc
     6121 agcagcaata gtcgaactcg atgccctggc cgatgcggtt gggtccgccg ccgaggatga
     6181 cggctttctt gcggtcggac gggcgcgcct cgtccgccgg ggcgccggcg aacggcgcct
     6241 cgtaggtcga gtacatgtag gcggtgggag aggcgaattc ggccgcgcag gtgtcgatgc
     6301 gcttgtagac cgggcgcacg gccagggcgt ggcgcgcggc gcgcacctct tcctcgcgct
     6361 tgccggtcag ggtagcgagg cgcttgtcgg aaaagcccat cgccttgacc gtccggaagg
     6421 cctgcgcggt gtccggcagg ccgaggcgct tcaccttggc ttccagatcg acgatttcct
     6481 tcaggcgctc caggaaccag ggatcgatct tgcaggactg atgcacctcg tcgacggaga
     6541 agcccaggcg cagggcctgt cccacctgca gcaggcggtc cggcgtcggg gtgccgatgg
     6601 ccttgcggat ggcgttgcgg tcgtcgccct tgcccaggcc ctcgatctcg atctcgtcga
     6661 ggccggtcag cccggtttcc agcgagcgca acgccttctg cagggattca tggaaggtgc
     6721 ggccgatggc catggcctcg ccgaccgatt tcatcgacgt ggtcagcacc ggatcggcgc
     6781 cggggaattt ctcgaaggcg aaacggggaa tcttggtgac gacatagtcg atggtcggct
     6841 cgaacgatgc cggcgtgaca ccaccggtga tgtcgttggc gatctcgtcc agcgtgtagc
     6901 cgacggcgag cttggccgcg accttggcga tgggaaagcc ggttgccttg gacgcgagcg
     6961 ccgaggaccg cgacacgcgc gggttcatct cgatgacgat catgcgtccg gtggccggat
     7021 cgaccgcgaa ctgcacgttg gagccgccgg tctcgacgcc gatctcgcgc agcacggcga
     7081 gcgaggcgtc gcgcatgcgc tggtattcct tgtcggtcag cgtcagcgcc ggggcgacgg
     7141 tgatggaatc gccggtgtgc acgcccatcg gatcgatgtt ctcgatggag cagacgatga
     7201 tgcagttgtc gcgcctgtcg cgcacgactt ccatctcgta ctctttccag ccgagcacgc
     7261 tttcctcgat cagcacctcg gaggtggggg aggcatcgat gccgcgctcg acgatgtcga
     7321 tgaattcggc cttgttgtag gcgatcccgc cgccggtgcc gcccatggtg aaggacgggc
     7381 ggatgatggc gggcaggccg atgtcgtcca gggcctccag cgccttcgcc agcgttttca
     7441 catgatgcga gcgcggtgtt tcgaggccga tcttggtcat cgcctcgcga aacagctcgc
     7501 gatcctcggc cttgtcgatg gcatgggccg tggcgccgat catctcgacg ccgaacttct
     7561 caagcacgct gattcggccg cccctgccgt cgtccagcgt cttcttgttg agcgagaggg
     7621 cgcagttgag cgcggtctgg ccgcccatgg tcggcagcag ggcgaatccc ttcgcgcggt
     7681 cgccgcgctc cttctcgatg atcttcgcca cgatctcggc ggtgatcggt tcgacatagg
     7741 tcgcgtccgc cagttccgga tcggtcatga tcgtcgccgg attcgaattg accaggacga
     7801 tgcggtaacc ttccgccttc agcgccttga cagcctgcgt gccggaataa tcgaactcgc
     7861 aggcctggcc gatgacgatc ggacccgcgc caatgatgag aatcgtgtgg atgtctgttc
     7921 ttttcggcat gcgtcatctc aagccccgag gggcttttca aggttgcagg ccgaccgatc
     7981 ccatgcggag ggtccgcagg gcgggcagcc agtcggttcg tttgctaaag cgcctctata
     8041 gagcgcgccc ggcagttggc caagggaaaa gcgacgggcg cgctccggcc attctcaccc
     8101 cgacagcgcc gccttgacca gcccgctcgc cttgccgaaa tccatgcgac cggcgaactt
     8161 gccgcgcagc acgccgacga ccttgcccat gtccttcatg ccggcggcgc ccgtttcggc
     8221 gatggcggcg gcgatcgcgg ccttaacctc ctcctcgctc atctgcgcgg gcaggaagga
     8281 ggaaatcacg gcgatttccc cccgttcccc ggcagcgagc tccggccggc cgttcttttc
     8341 gtagacgtcg gcggattcct ggcgctgctt gatcatcttc tgcaggacgc cgagaatttc
     8401 ctcgtcggag gccttctcgc gtccggcgcc gcgcgcctcg atatccttct ccttcagcgc
     8461 cgcgatgatg aggcgcaccg cgccgagccg ggccttgtcg ccggccttca tcgcatcctt
     8521 catcgcctcg gtgaatgtct cgcgcatcgc ctgtctccgg ttcagatgtg tcgttggccg
     8581 cgttgacctt gtcggccaag acccttgacg ccgaaagtcg caataggcga ttttcggtcc
     8641 agctcaaaaa tttctccaaa tcggaccatg agaaattttt gaaaggaacc aaatctggaa
     8701 gggtttccct tcccgagatt tggtgtaagg catcggcctt cagaagacat gcgccggcgg
     8761 attgcaggtg cggcatgggc cgcgccgctg ttcttgcgcg ctggagtaga cgaagccatg
     8821 gccacagcac aagcacagaa cgccgattcc tgggcggaaa ccaaagccac cgccatgctg
     8881 gtgctggcgg acggcacggt gatcgagggt gtcggcgccg gggcggaagg catcgccgag
     8941 ggcgaggtct gtttcaacac cgccatgacc ggctatcagg aaatcctgac cgatccgtcc
     9001 tattcgggcc agatcatcac cttcaccttc ccgcatatcg gcaatgtcgg tgtgaacgag
     9061 gaggatatcg agaccgtcaa catggcggcc tcgtcggggg tgcgcggcgc ggtgctgcaa
     9121 tcgccgatca ccgaaccttc gaactaccgc gccacgaaac atttcgacgc ctggctgaag
     9181 gcgcacggca ttgtcgtcat aaccggcgtc gacacgcggg cgctgaccgc gcttattcgc
     9241 gacaagggca tgcagaatgc cgtcgtcgcc catgcgccgg atggcaaatt cgacgttccc
     9301 gccctcaagg cgaaggcgaa ggcgctgcct tcgatgaccg gccttgacct ggtgccgcag
     9361 gttgccgcca cacagcgctt cacctgggac gagaccacat gggcctggga caggggttat
     9421 ggccggcagg acaagcccgc cttccacgtc gtcgcgctgg attacggtgt gaagcgcaac
     9481 atcctgcgcc tgctcgccga ccggggctgc aaggtcaccg tcatgccgcc gaccgccagc
     9541 gctgacgagg ttctggcgca gaagcccgat ggcgtctttc tcgccaacgg acccggcgac
     9601 ccggcggcta cgggggagta ttccgttccc accatcaagg cactgctgga caggaggatc
     9661 ccgacgttcg gcatctgcct cggccatcag atgatggcgc tcgccatcgg cgcgaagacc
     9721 ctgaagatgg cgcatgggca ccatggcgcg aaccatccgg tgaaggagca tgcgacgcag
     9781 aaggtcgaga tcgtctcgat gaaccatggc ttcgccgtgg atccggcgac gctgccgaac
     9841 aacgccgagg agacccacgt ctccctgttc gacggctcga actgcggcct gaagcttacg
     9901 gaccgcccgg ccttctccgt gcagcaccac cccgaggcct cgcccggccc gcaggacagc
     9961 cactacctgt tcgaccggtt cgtggagatg atgaaggcga acaaggccgc gtgaggcgcc
    10021 gcgcgggacc gccgccatgc gcctcgccgt cctcgccgac atccatggca acatccgcgc
    10081 gcttgaggcg gtgatagcgg cgttgcgcca cgagacgcct gaccggatcg tcaacctcgg
    10141 cgactgcttt tccggcccgc tccatgcggc ggccgtcgcc gaccggctga tcgagctgga
    10201 ctggctgacc gtgcgcggca atcacgaccg ctggctgctc gagacgccgc cgcaggacat
    10261 ggggccgtcg gaccgccatg cattcgccca gctcgacacg aaggaggagg actggctgcg
    10321 cgacctgccc gccaccgccc gcctcgatgg cgatgtgctg ctttgccatg gcacgccggc
    10381 cagcgacacg acgtacctca cagaaacgct ccgcggcgac agcgtgcgcg aggcaacgcc
    10441 ggatgaggtc gctgcccggc tcggcggcga ggccgccgcg cttgtcctgt gcggccatac
    10501 gcacatcccg cgcctgatcc accggcccgg cggcggcctc gtgctcaatc cgggaagcgt
    10561 cggcttgccg gcctacacgg atgacgaccc ctggcacttc gtcgaaacgg gaacgccgca
    10621 cgcccgctat gcattggcga cgcgggcgtc cggcggctgg tccgtggagt ttcggcgcgt
    10681 cgtctatgat tgggaggccg ccgcccgcga tgcggccaag gccggcaggc cagactgggc
    10741 gcatgcgctc cggaccggac tggcgatgaa ataggcaagg cccggcgcca acgccgggcc
    10801 ttccgtgagc gtgctcgcct gcagggcaag actcaggtga caagcatgtt ggcgaagagc
    10861 gtgcgcacgt cgacgttctc gaaggtgatg gcgtgaatgg cgccgccttc gttcgccaca
    10921 tagagagtca cgccgaagcc ggtcgggtcg agcgcgaccg ttgaccgcga caggatcgaa
    10981 tgggtgatca ccagcgtgtc cccggcggta aagtcggtga caacatcgtg ctcgttgccg
    11041 aagatttcgc ccaactcgtt gaagtagaac ctgtcggcgc cttcgccgcc tgtcagggta
    11101 tcgttgccat agccgcccca gagctggtcg tcgccggcgt cacctatgac gtagtcgtcg
    11161 ccatcgttgc cgaagacctg gtcgtcattc tcgccgccgt agaccacgtc gttgcccatg
    11221 ccgcccagca gcgagtcatt gccgatgtcg ccatagaggt agtcgtcacc ccagacgccg
    11281 cgcagcgtgt cattcccggc tccgccgacg atggtgtcgc tgccgctgtc caggtcctcg
    11341 ccgccgacca gcgagtcatg gccgccgccg ccgaagatgt agtcgtcgcc gccgcggcca
    11401 tcgattgtct cgccgccgtt ggtgccgatc agccagtcgt tgcccggcgt gccggttgtg
    11461 ttcacggtct ggccgggagc ggtgaggccc ttgctgttgt accgcgcgat gagggcaaca
    11521 tcttcagaaa tggtgattgc cttctcttcg gcgtaccagt ttttcagcgt ttcgaagccc
    11581 atgatcaatt ctcctctgtt tccaatgaag cgttgcttcc acgaggagaa cgatgtcaaa
    11641 agcgtcgaat taattctgtg attgtgcgca catgatgaca tatttattta ctattacaca
    11701 caaagttaaa attttatact attattttga agcgaggctt ccaacgaaaa gcatggcccg
    11761 gccgatccag atgcgcgcct cttccgggct ctgcagaaca gcgccggaca cggcgatcca
    11821 gccggtcagt tccttgccct gcattacaaa ggcactcgcg cccttttcgc gcagagcatc
    11881 ggggtgcgcg tcgacgccaa cgcggaagat cacatcgccc ttgtaggtgc cgcacagcat
    11941 gttgccgttc aaaaggaaac aggtgccgcc gaacatcttc ttctcgggaa ggatgcgtgg
    12001 agcgagcgcc tcgcggatca gttgcgccag cgaagcctcc gccgcccggg ttgtagggtc
    12061 cgcctttgtc cggcgcggct tcttgcgatc ggcggcaggc ctgccggatt cgtgcatcgc
    12121 atccctcggt cgggccggct gccttgcgct gcgtcaaggt tctttcagcg gctctggtgt
    12181 caatgtatca catgactcgc acgcttccga tcctcgccgt cctgctgctc tgcgccgcgc
    12241 cggccttcgc ccagacaacg aaaggtcaga tcgaggccgg tcgttcgctt gccagggagc
    12301 attgcgctgc ctgccatgcc atcggcaagg ctgatcgctc gaagcggcgc ggggcgccgg
    12361 ccttccgcac catcggccgc acctacgacg tcgacaacct gcaggaggct ctggccgagg
    12421 gcatctcggt cggtcacaag ggcgtcgaca tgccggaatt ccagttttcg ccggaccgcg
    12481 tcgatgcgct gatcgcctat ttccggtccc tgcggcgctg atcgcgcggg ccggagcagg
    12541 gagaagactt tcccgcgcgc aggcgttaag acagcgcgac ggggaggggc ttcatgtcct
    12601 ggatcgagtt ctggaaccaa gacacgtcca tctatgtgag tgcacggcac aaggcgctgc
    12661 actacagaca ggtcgcgcgt gacatcgccg cgctgatcga cagccccgat gccgtcgtgc
    12721 tcgactatgg gtcgggcgag gcaacctcgg cggacatcgt cgccgcgcgc tgcgccgcgc
    12781 tcgacctttg cgacgccgcg cccaacaccc gcttgaaaat ggcggaacag tttgccggca
    12841 tcgccaacat ccgcgtgctg tcgaccgagg atgtgcagtt cggcgaggat gcgcactacg
    12901 acctgatcgt cgccaattcg ctgctgcagt atctggatga gccgactctc gacgggttgc
    12961 ttgacctgtg ggcgcggcgg ctgaagcccg gcggccggct gatcctcgcc gatgtcatcc
    13021 cgccaggcgt ttcaccggtg acggacgccc tcgcgctgct tcggttcgcc ttttccggcg
    13081 ggttcttcat ggccgcggtc ctcggtctcg cccgcacctt cttttcggat taccgcaagc
    13141 tccgggggga gctgggcatt tccatgcatg ctgagtccgc cctcctgacc cggttgcgca
    13201 atcgcgggtt ctcggcgcgc agaatgcccg acaatatcgg acacaatccg gcgcgcatga
    13261 cgttcgaggc gatcaggccg ggagagcagg gccggtagtg tttgctcact tgtcgtctct
    13321 ggcgtcggcc ggcgagtcgg gacgtgaaag gatgaacgcg cccgcgccga caaggcacag
    13381 ggcctgaacc gcaagcacct cccagcgcag cctcaggagc agcgagagca gcaacaccgc
    13441 gcccatcgcg gcggttgcca tcagcttggc ccggcgcgag atggcgccat gggcgtgcca
    13501 atccctgacc ggaggcccga atgtgggatg ttcgacgatc cagcgatgca ggcgggggga
    13561 agatttggcg aaggctgccg cagacagcaa catgaatggc gtggtcggca ggatcggcag
    13621 aaaagccccg agaaggccga ggcccaggga aatccagccg agagagaacc agagcaggcg
    13681 tttcaccggg tgtctccgaa ggtccagatc ttgtgggcga cgaagctcca gaccagcacg
    13741 atgccggtgg tgacgatctg catgggcaga tagggcaggc ccatgcgctc cacgaacagg
    13801 tgcatgaacc cgaaagtcag caggaagccg acgccggcga ccgcagcgaa gcgccagccc
    13861 gcctcactgt gcgggcggtc gctggcgtag gtgtggtggc ggttgagcgc gtaggacacg
    13921 acaccgccga gcacgtagcc ggccagtgtc gccggaaccg gtttcagtcg caccatctcc
    13981 acgccgatga cgagggtgga atagtgcacg agggccgcca cgaggccgac gccaaagaag
    14041 gctgcgaact ggcgcaagag ggcaagcatg cccgctggct tattgcgaat cgcccggttt
    14101 cgccagttcc ggcttgcggt tcgcgatgtc gacctgctgt ttggttctcg gctcatagga
    14161 gagccggtgc tcggggcagc cgcaatccgc cggatgggtg ccccgctttg cggcgttgcg
    14221 ccggatggcg tcggcctgcg cccagatttt ctttagcgaa ccgaaaatgc tctcccgggg
    14281 cggcacgtcc gacctcacga ctggaatcgt aacagtatta caatagcgct gccgggcgct
    14341 ctttcaagcg cccggaaggc cacgttcgga tcggatttca gaccaggatc atggccagcg
    14401 cggggtcgtg accgatcagg tgaacaaccc ggttgccgcc tgcatcgagg aggaaggagc
    14461 cgccctgctg gtccgatatg gcccggtagt ccgcgacgtt cccctgcaca tcgatgacgt
    14521 cgccttgccg gaaactgtag tcgaggatga cgtccagccc cttcctcggg ccatcgctga
    14581 cgatgaacca gtcggaaccg gtgccgccct gcaggatgtt gaagccggcg ccgccgtgga
    14641 ggatgtcgtt gccctcgccg ccgatcagcg tgtcgttgcc gccttcaccc atgaggaagt
    14701 ccgatccacc gccgccggag agaaggttcg cgccgctgtt gccctggatg gcgtcggcga
    14761 agatcgagcc ggtgacgtct tccacgccgt ccacaaagcc gttgccgttt tcgctcagat
    14821 acaccgcggc gcgcagggag atttccagcg gaccgccctg ccagtccgag aaatctagca
    14881 tgtcgcggcc ggtgccgccg agcagggtgt ggcttcccca gttgaccttc agaaggtcgt
    14941 tgccgtcgcc gccttccaaa aggtcactgc cgaattcggc gaccagggtg tcgttgcctt
    15001 gcccgccata aatccgattc cggcccgcat cgccggtcag atagtcgtcc ccctcgccac
    15061 cggacagcgt gtcgttgccg gttccgccgt acagcgaatc attgccgtgc tcgccgaaca
    15121 tctggtcgtc gccggtgccg ccatggagga tgtcattgtc ctgtccgccc cagatcgtgt
    15181 cgtttccggc gtcgccgtgc agcacatcct cgccggcttc acccgtcacc cagtcgttgc
    15241 cgtcgccggc gagcacgagg tcgtccccgc gtccggcccg gatttcgtcg ttgccaccgc
    15301 cggcaaagat ccagtcgtca ccgctgccgg ccagaatggt gtcgttgccg cctagggcga
    15361 tgatccattc gccggcggcg atcgggcgac ggtcgatgct gtccgcgcca tcggttccga
    15421 cagtgatggt gccgtcgttc aacgggacga accgtggggc atgaaacact ctcatgactt
    15481 cctcctgcga gatgccccac cgtcgttgat cctttgtctc ccaggtgcgt ccccggtgca
    15541 gtgcatcggc gcacaggccg tggtcattgg gaaaaaagga aaggccgggc gcgcctgttt
    15601 ggtacggatc aaaagcattg ctgtcctctt tccgcgatca gaggatgtcg atcccgttgg
    15661 ccagggtgac gttcagcacc tgcatgatgg cgttgccggc cacatcgagg atcatcacgt
    15721 ccgcgccgga ctggatcgcg cggtagctgg cgctgtcgcc ggcgacatcg atcacatcgc
    15781 cttcgtggta gtgatagtcg gtgacgacat cgaagcctcc cgcagcaagg cctgcggcga
    15841 tgaaacggtc cgaaccctcg ccgccggtga gaacgtcatc cagcgcgccg ccgtcgaggg
    15901 tatcccgccc gccgccgccc tcgagcgtgt catagccgcc agcgccgcgc agaatgttgt
    15961 cgccgccgtt gccgacgatg agatcggacc attgcgagcc tgtggcgttc tcgatgtcgt
    16021 ggagatagtc ggtcacgccg cgcgactggg cgaagccgcg atccagccag atgccaaagc
    16081 cggtgtgctc cagattggcg cccgacgcct gataggtgtc gttgccttct tcgccataca
    16141 gaaagttggt gcccgtgccg ccgataagga gatcgtcgcc agtcccgccg aacagcagat
    16201 ccgagcctga accgccatcg atcgtgtcat tgtgcgcgcc gccgaagatc gtgtcctggc
    16261 cgccgccgcc cagcacacgg tcgttcccgt cgccggcggc gacatagtcg tcgccatcgc
    16321 cggcgtctat gacatcacgg ccggagccgg catagattgc atcattgccg tcgccggagc
    16381 tgatgttcca gtcaagccgc gaccggccct tgtcgattac atcgtttccg gcggtaccca
    16441 gcaagaaatt tggcagtgga attgggcgaa caaggccaac catggatctg ctccacgttc
    16501 aatgccctcc gtcgtcccat ccatcggtgt ttctagatga ttggccgggt gtgcgcggtg
    16561 cgttgaaaca ccccgcgaaa gccggtgaca caggccggct tgcggcttaa cactctggca
    16621 ggaggatcca acgttgatgc ccgataccac cctcattctc cgtcagcatg ccctgcgttc
    16681 catcatcggc gaactcggcg atctggcgct cgcgtatttt cgcaaccgcg acagtctggg
    16741 cgtcaccttg aagggcgcgc aggactggct gaccgaggcg gacggcgcgg tcgagcggct
    16801 gtttcgccag cggatcgcgg aagcgtttcc gggcgatggc gtgttcggcg aggagcaggg
    16861 cggcacggcc ggtgatgccg cggccgagaa cctgtggatc atcgatccga tcgacgggac
    16921 cgccaatttc gccacgggcg accggcagtg gtgtgtttcg gtcggcttcc tgcacagggg
    16981 cgtgccggaa ctgggcgcgg ttctggctcc cgcgcaggag gaactgtttc ttgcccggcg
    17041 cggtggcggc gcaacgctga acgggcagcc gatccgggcc aaccccatca ccgacctgac
    17101 gcgcgcgtcg gtcgagatcg gctggtcgaa gcgtcggccg ctcgcgctct atatcgacgc
    17161 cgtccgcgcg acgatggagg caggcgccgg ggtcaagcgt tcggcctccg gcgccctcgg
    17221 tgttgtgcat accgcgatgg gccgcgtcga tgcctatgcc gaggcccata tcaattcctg
    17281 ggatgttgcg gcggcccttg tcatcgcgcg cgaggcggga tgctacgcca atgacttctt
    17341 ccggggcgac gccatcgtga agggcaatcc gatcctggtc gccgccccgg ggatcgccga
    17401 agcggtcatc cgcacgacgc ggttcctgga ttgattgcgg cggatcatcg cgccggctca
    17461 cgcccgcccg atagtgtgcc ggctaaggag atgccggcgt gagtatcgag atcgatctgt
    17521 tgcagcggct cggcgtggcg gtggcgatcg gcgcggttgt cggcgtcgag cggcactggc
    17581 gcgagcgcga cgcgggggaa ggccgacgca cggccggcct gcgcacctac tcgctcacgg
    17641 gtcttctcgg cggctgcgcg ggcctgctgg agaagcagct tgccgggcat gatggtctgg
    17701 tgatcggtct gatgtttctc ggattcgccg tggtcttcgc gcagttcaac ctgcgcgagg
    17761 cagcggagga gcgcaatttc
//
LOCUS       DAOMXN010000028        17765 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_1814_length_17765_cov_11.763919, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000028 DAOMXN010000000
VERSION     DAOMXN010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 17765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17765
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1814_length_17765_cov_11.763919"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..1154
                     /locus_tag="PK812_04195"
     CDS             111..1154
                     /locus_tag="PK812_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011425548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRJ68788.1"
                     /translation="MTEKVRIAVAGAGLIGKDHIRRVAESDVAMLCAVIDPAPAGAEL
                     AARFGAPHFTDLAEGLKASRAEGVILATPNTLHVPQGLIAVEMGLPALVEKPFADTVE
                     AGERLVEAAAKKGVPLLVGHHRRHSPLMRRAKETIASGKLGKLVAVNGMYWFRKPDSG
                     YFDGPGAWRKEPGGGPILINLIHFIDDMRNLVGGIESVQAMAANAVRGFAVEDTAAVM
                     LRFRNGALGTITLSDSAASPWSWEMTSAEQKWYPASGENYLFVAGTKGSLGLPKQDFW
                     HYEGEPHWFKPLALTSARIPEQDPLVNQIGNFCDVIRGKAEPVASGAEGLATLKATLA
                     VKQAAETGQAVRL"
     gene            complement(1149..1364)
                     /locus_tag="PK812_04200"
     CDS             complement(1149..1364)
                     /locus_tag="PK812_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68789.1"
                     /translation="MIRPEVTELSHDLLQEKVRMAIVFKNHNGETLQHIQLVFPLHMD
                     LQDTELKLKQTAIEQARKFLRAAADGL"
     gene            complement(1447..2187)
                     /locus_tag="PK812_04205"
     CDS             complement(1447..2187)
                     /locus_tag="PK812_04205"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HRJ68790.1"
                     /translation="MRLHGKVAIVTGAAQGFGEEIARTYAREGAKVAVLDIKADLAAK
                     VAKDIGANAVPIACDVTSLSAVKDAVAATVKAFGRLDIVVNNAGWSYSNRPVLETPEA
                     DFDKVFAINVKSIYNFVTAGVPHMTNGGSIVNIGSTAGLRPRPGLVWYNATKGAVHIA
                     SKTLAVELAPKNIRVNVIAPVAGETPLLQTFLGNDTPERRAQFLATIPLGRFSQPRDI
                     ANAALFLASPEAEFITGTVLEVDGGRCV"
     gene            2317..2667
                     /locus_tag="PK812_04210"
     CDS             2317..2667
                     /locus_tag="PK812_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRJ68791.1"
                     /translation="MQEAVRRGETWIAAWNSRDLERILALYDDDAVMASEKIRMLGLD
                     PSGTLHGKERLRAYWQIGLARRPGLRFDLVAVSASPDSIVVRYRDEHGHEVDEYLRYA
                     MDGLIVQGSANHPA"
     gene            complement(2677..3441)
                     /locus_tag="PK812_04215"
     CDS             complement(2677..3441)
                     /locus_tag="PK812_04215"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRJ68792.1"
                     /translation="MLLDTSMLLAGLGCAVIIGMAKGGLSGGLGTLVVPILSLSMDPR
                     AAAALVLPLLIISDFFAIWAFRHRWHWGHTWVLLVGAALGVVFGTFTFSWVSEPAMRL
                     MMGVISVAFVAANLTGATRAADTPQPPALGAGVFWGALSGFTSFTAHAGGPPVQIYLV
                     PRGLDKRTFQATVTLFFTILNLAKVPPYMALGQFTQPIMLTALVLMPAAAIGVFIGFR
                     ITGLIPEKPFFRLVLFLLGITGLKLMWDGLTGLGWL"
     gene            3568..4179
                     /locus_tag="PK812_04220"
     CDS             3568..4179
                     /locus_tag="PK812_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019903008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HRJ68793.1"
                     /translation="MREERRERDLEAGAVALSGQLGKTIQRLRKAYNLSLSELSEQSG
                     VAKSIISQIERNETNPTLATIWRLSQALDVSVDRFLGQADDAPFVQKSTVHDTPILMS
                     EDGKCKLAIIGWLKTVEWLQWYDMRAEPGGVLDSDAHQRGSIECLSVIEGEMEVEVAG
                     VTEKARAGETLRYRCDRPHTIRNVGPDPAHATMVVILKAAVME"
     gene            complement(4231..4647)
                     /locus_tag="PK812_04225"
     CDS             complement(4231..4647)
                     /locus_tag="PK812_04225"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRJ68794.1"
                     /translation="MLARRTVLALPVAAFAAPASAQGALPSVTVSEAQERLASGKSVL
                     VDIRRPEEWAETGVASGAVRLDMTADDFLPKLQSLQKANPGKDIDIICRTANRTAFVQ
                     SELAKRGWKGIVNVRGGMAGRAPDIGWIAAKLPIVK"
     gene            complement(4650..5030)
                     /locus_tag="PK812_04230"
     CDS             complement(4650..5030)
                     /locus_tag="PK812_04230"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRJ68795.1"
                     /translation="MFTSLMKSLSGALTPAPALPSVSVQEARQRVESGAAILVDVRGP
                     SEWAQTGVALGAECIQMGTPDFVARLEALRRRHPARAIDLICRSGARSGSVQRALGDM
                     GWTNVVNVRGGTSSWMAAGLPMQR"
     gene            complement(5279..6214)
                     /locus_tag="PK812_04235"
     CDS             complement(5279..6214)
                     /locus_tag="PK812_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRJ68796.1"
                     /translation="MIGRLVKLGAMLGLLGLAGFFALTEPAVLSALKGSALPVASGAP
                     DLANGKTMFHAGGCASCHATEKQDDKTRLGGGHALHSPFGTFYVPNISPHPRDGIGGW
                     TAEQFLRAMREGVSPEGRHYYPSFPYTSYQRMTAKDVGDLFAFLKTLPPIEGRIRDHD
                     LPFPFNIRRSLGGWKLLFADGQVYRPDPAKSAEWNRGAYLVEGPGHCAECHSPRNPLG
                     GITSGKRFAGGPDPEGKGYVPNITPHETGLKSWTKKDLAYLLESGFTPDYDSVGGSMT
                     PVIGNTGQLSAQDRNAMAEYLSTLPAIEGRKKGAK"
     gene            complement(6291..6725)
                     /locus_tag="PK812_04240"
     CDS             complement(6291..6725)
                     /locus_tag="PK812_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRJ68797.1"
                     /translation="MKRMIIAAAVLTLGVTAGIAQGDPIAARKAMMKEVGGAMGPMAA
                     MNKGERPFDLATAKASLGTMNKASKAFGPLFPATAKSGGESTADEKIWSDAAGWNAAL
                     KKFETDTAAAVAAVKDEASFKAEFGKVAANCGGCHNTYRIKK"
     gene            complement(6873..9176)
                     /locus_tag="PK812_04245"
     CDS             complement(6873..9176)
                     /locus_tag="PK812_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HRJ68798.1"
                     /translation="MKPMKFGMGQAVRRVEDQRFITGSGHYATDYRPDGVTYAAFVRS
                     PHAHATFAIRNVEAVRALKGVLLVLTPEDVAGLNPIPCQAILKNSDGSSMVRTPYAAL
                     AQGEVRHVGDTVAMVVAKSLAQAKDAAEALEIDWKAMPAAVGIKAAMKKGAPAVWAEV
                     TGNIALDSHIGDKAKTDAAFARADRVVKLTIVNNRLVANYMEPRVCTAEYDTAKKRYT
                     LTLGSQGSHGIRDTLAKILGEKSENIRVVTPDVGGGFGNKAFMYREYPLLAVAARKLK
                     KPVTWTQERSEHFLTCAQGRDNIATAEMALSSSGRFLAMRVDLLADFGAYLSQYAPYI
                     PFLGGTMTTGVYDIPAAHFRVRGIYTHTVPVDAYRGAGRPEAAYLIERLVDAIALETG
                     RTPDRIRSINYIRPGRMPYATPVGRTYDSGDFEGVMRRAMEVSDWSGFRKRAAASKKR
                     GRLRGIGLATYIEACGGGGAEPAYVALEKDGSITIRIGSQSNGQGHETAYAQLASQHL
                     DLPLSKIRVLQGDTNQTPTGSGTGGSRSIPVGGAAVDVAAARLAENLRKIAADELESA
                     PSDLEIADGRVRVAGTDRAIDFAGIARSRKATAELLNTTGSFAPPEATYPNGTHVCEV
                     EIDPDTGSTQVLSYVIVDDFGVTLNPLLLAGQVHGGTIQGIGQALLENTVFTPEGQLL
                     TASFMDYAMPRAADVPSLHFETRNVRCVTNSIGVKGAGEAGAIGSCPAVINAVVDALN
                     RGFGIRHIDMPATPEKVFAAIRSAKAR"
     gene            complement(9336..9656)
                     /locus_tag="PK812_04250"
     CDS             complement(9336..9656)
                     /locus_tag="PK812_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012456719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRJ68799.1"
                     /translation="MTIIDLSLDDVRQGLADGTLILVDVREDNEFAMGRIPGSLSMPL
                     SRFDVNWLRQFDGRRVVFSCAAGVRSQHALMAAQSAGLGIEEHYRGGFKDWIMAGGPV
                     DSGA"
     gene            complement(9785..10603)
                     /locus_tag="PK812_04255"
     CDS             complement(9785..10603)
                     /locus_tag="PK812_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRJ68800.1"
                     /translation="MTALSVTFWGVRGSVPTASAEYMEFGGNTPCVEIRAGKQVFVLD
                     AGTGAVALGRKLQEEGVKDIILLFSHLHHDHTAGLPFFAPIHDKSVSITMYCGNLGGE
                     SAQKHLDQVFGPPFFPIVFSRFAAKIRSEGFLAGETFTFGDVTVTTQPLNHPGGSTGY
                     RFERAGKSVSYVTDVEHVEDAPDPDVRRFVRNSDLLIYDTMLTEDELVSCRGWGHSTW
                     KAGVKLAQSAKVGQLAAFHHNPQFNDRRLAKLDGKIRSKMAEAFFVKEGQSFEY"
     gene            complement(10712..11791)
                     /gene="amrS"
                     /locus_tag="PK812_04260"
     CDS             complement(10712..11791)
                     /gene="amrS"
                     /locus_tag="PK812_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HRJ68801.1"
                     /translation="MIGRYFRKLTDGRVECTLCPRLCKLNDGQRGLCFVRQRVGEAME
                     LTTYGRSSGFCVDPVEKKPLAHFLPGTPILSFGTAGCNLACSFCQNHEISKSREMDTL
                     AQEASPEAIARAALAHGCRSVAFTYNDPVIFLEYAVDVAAACRAVGIRTVAVTAGYIS
                     KQARGEFFGAMDAANVDLKAFSEDFYRQHTRSSLADVMDTLVWLVAETKVWLELTTLL
                     IPGENDSEQEIRALSRWVATALRPDVPLHFSAFHPDHRMLDKERTPHRTLAHARAIAI
                     SEGLKHVYVGNVHDLNRQSTYCTGCGRRVIARDWHLITDYRLDEGGRCLDCGTPMAGL
                     FDGPRGTWGRKRQPIMMRNYASTAA"
     gene            11900..13324
                     /gene="amrB"
                     /locus_tag="PK812_04265"
     CDS             11900..13324
                     /gene="amrB"
                     /locus_tag="PK812_04265"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HRJ68802.1"
                     /translation="MSAPSISRAAPIELDVLPAQVAGTFYPEDRQGLSNQLSRCIVSA
                     VQSSIRAPKIIIAPHAGFTFSGGVAASAFHGLANRSDLIRRVVLVGPAHKMSFKGIAL
                     HPARAWRTPLGDVTVDWTMLRRVQPLPGVVMDDRPFLREHALETHLPFLQVSLKQFEI
                     CPMLVGDASPELVAQALERVWGGPETLISVSSDLSHFLSDTAARTIDQETRRIIETLD
                     AERLNATRACGHRAIAGALRLARTHDLRVTGIDLKNSSDMTDERERVVGYGAFAMEYA
                     AQAELAPVERKLLLSTAARSLVHACQNNGATPDVAINGTLPATLSAMRATFVSLESGG
                     RLRGCIGSLKPHRPLLLDVIANTVKAGFADPRFPKLTLQEMSGLTLSIAILSHPREME
                     FDSESALIKDLSPDRDGLIIEDQGRSALFLPQVWANIPDPTVFLKALKQKAGLPADHW
                     SPTFKARRFAAEKFGRPIVDILKG"
     gene            complement(13340..14359)
                     /locus_tag="PK812_04270"
     CDS             complement(13340..14359)
                     /locus_tag="PK812_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020179114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRJ68803.1"
                     /translation="MTVSDTPLPASPDPADAPRRPRPILGILLKVLSALSFTLMAAIV
                     RHSATGGIRFNPPVGQLVFFRSFFALVPVLIWLTYARPLSAAFATKNLKGHIRRGIIG
                     TLGMFLGFAGLVYLPLPDATAISYAAPLLSVLLAAVILKETVRLYRWTAVVIGLFGVI
                     VMLLPHLALGGKHVPPGAATGVWLALGGALCAAVATIEVRRLTATEHTAAIVVYFSLF
                     TTVAALVTFGLGLVRPEMAWHWPGARDFWMLAAIGMLGGIGQILMTHSYRYADASVIA
                     PFDYTSMIWALLIGWLLFGEIPEKLVLIGAVIVIASGVFVILRERQLGIERKRMRRTG
                     PGRPL"
     gene            complement(14464..15264)
                     /locus_tag="PK812_04275"
     CDS             complement(14464..15264)
                     /locus_tag="PK812_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68804.1"
                     /translation="MTHGKLPAPISQADYDTIEAAVMETERGRWFLSEYARRNRHADT
                     ERVLGAIEKLHHNLQRQRTEARLERMRLELVEMARAIAITHVEIGRIRPQGLAATRLN
                     VASGELNAIVRATEEATSSILSAAEQVQQQAWTMRELGAPEAHCDNLDACATEIYAAC
                     GFQDITAQRTQKVIETLNYVEGRIKQIMQAWNVESPAAASEPTGQPASFWPDDAGRQE
                     RIDRMLELPDAEPPAGEPQPRQTSAPARTGAMQPLDDLPTAERLRLFS"
     gene            15497..16612
                     /locus_tag="PK812_04280"
     CDS             15497..16612
                     /locus_tag="PK812_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018264481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family lipid ABC transporter permease
                     subunit"
                     /protein_id="HRJ68805.1"
                     /translation="MTIQRAGDGAALAFGGDWSASNAHECEAVLESATGRIGPVKWVQ
                     IDVGGIGRLDTLGAWVISGLRHQLESAGAKVSLVEARTPHRILFEEVSYRPLPESPKR
                     REFIAVDMLADVGRAMDNAFQDLINGIAFLGAFMSGFANVILGRSRFRIASLVAQLER
                     VGLRSVPIIVLISVLVGGIIAQQSVFQLQQFGTTHFVADLLGVLVLRELAVLITAIMI
                     AGRTGSSFTAELGSMKMREEIDALKTMGLDPIEILVVPRILALLIGLPLLTFISEMAM
                     LFGGGVVAMVYGGVSPDVFLSRLQNIIGLNTFVVGMVKAPFMALVVGVIACLEGMSVG
                     GSAESLGARTTSSVVKAIFMVIVVDGLFAMFFAAIKY"
     gene            16620..17438
                     /locus_tag="PK812_04285"
     CDS             16620..17438
                     /locus_tag="PK812_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRJ68806.1"
                     /translation="MSEHAEPDEKRDKVIRVRNLTVGFGEKLIMKGLNLDVYRGEILG
                     FVGASGTGKSVLMRTILGLVRQREGNIIVFDKDLDTLTQRRREAVERRWGVLFQHGAL
                     FSSLTVRQNIQMPMREYLSMSGRLLDEMAALKIELVGLKPDAADKYPSELSGGMIKRA
                     ALARALALDPEIVFLDEPTSGLDPIGAQEFDDLIATLQRTLGLTVFMVTHDLDSLAGV
                     CDRIAALAEGKVIAAGPIETMMESDHPWLKAYFHGSRARRGSLETGRFRASLRS"
     gene            17438..>17765
                     /locus_tag="PK812_04290"
     CDS             17438..>17765
                     /locus_tag="PK812_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRJ68807.1"
                     /translation="METRANYALVGLFTLAVIAASFGFIYWFRSASNAGERAPYRIVF
                     NGSVSGLSKGAQVRFNGLRVGEVMAVELMPDDPSRVSVLVAIDPKVPMRTDTRARLDV
                     QTLTGAA"
BASE COUNT         3094 a         5587 c         6001 g         3083 t
ORIGIN      
        1 cacgccgagg cgcccgtctt gcgcgacgtt ggagcagggc gcttggccgc ctgccatttt
       61 ccgctgtagt agttgtctga caacggtccg caggccgctc ggagacagac atgacagaga
      121 aggtgcgcat cgccgtcgcc ggcgcggggc tgatcggcaa ggatcacatc agacgcgtgg
      181 cggaaagcga cgtcgcgatg ctctgcgcgg tgatcgatcc ggcgccggcg ggggcggaac
      241 tggccgcacg tttcggcgcg ccgcacttca ccgacctcgc ggaaggcctg aaggcgtccc
      301 gggctgaggg cgtgatcctt gccacgccga acaccctgca tgtgccgcag gggttgatcg
      361 ccgtggagat ggggcttccg gcgctggtgg aaaagccctt cgccgacacg gtggaggccg
      421 gcgaacggct ggtggaggcc gccgcgaaga agggcgtgcc gctgctggtc ggccatcatc
      481 gccgccattc gccgctgatg cgccgggcga aggaaacgat cgcatccggc aagctcggca
      541 agctggtggc ggtcaacggc atgtactggt tccgcaagcc ggacagcggc tatttcgacg
      601 ggccgggggc ctggcgcaag gagccgggcg gcgggccgat cctgatcaac ctcattcatt
      661 tcatcgacga catgcgcaat ctcgtcggcg gcatcgagag cgtgcaggcg atggcggcga
      721 atgccgtacg cggctttgcc gtcgaggata cggcggcggt gatgttgcgc ttcagaaatg
      781 gcgcgctcgg cacgatcacg ctctccgaca gcgcggcttc gccctggtcc tgggaaatga
      841 cttcggcgga gcagaaatgg tatccggcga gcggcgagaa ctacctgttc gtcgcgggca
      901 ccaaggggtc tcttggcctg ccgaaacagg atttctggca ctacgagggc gagccgcact
      961 ggttcaagcc gctggcgctc acatccgcgc ggattccgga gcaggacccg cttgtgaacc
     1021 agatcggcaa tttctgcgac gtgatccggg gcaaggccga gccggtcgcc agcggggcgg
     1081 aagggctagc gacgctgaag gcgacgctgg cggtgaagca ggcggcggag accgggcagg
     1141 cggtccgtct atagcccgtc ggcggcggcg cggagaaact tgcgcgcctg ctcgatggcg
     1201 gtctgcttca gcttcagctc ggtgtcctga aggtccatgt gcagcggaaa gacgagctga
     1261 atgtgctgga gcgtctcgcc gttgtggttc ttgaagacaa tcgccatgcg caccttctcc
     1321 tgaagaaggt cgtggctcag ttcggtgact tccggccgga tcatcgtgct tccccccaag
     1381 gacgttcatg ggggaaaatg tgtcgaccgg ccggcgctca cattgcggta gcgcaatccc
     1441 ttgcggtcac acgcaccgcc cgccgtccac ttccagcacg gtgccggtga tgaactccgc
     1501 ctccggcgag gcgaggaaca gcgcggcgtt ggcgatgtcg cgcggctgcg agaagcggcc
     1561 gagcgggatg gtggcgagga actgggcgcg gcgctccggc gtgtcgttgc cgaggaacgt
     1621 ttgcagcagg ggcgtctcgc ccgcgaccgg cgcgatgaca ttgacgcgga tgttcttcgg
     1681 cgcgagttcg accgccagcg tcttcgaggc gatgtgcacc gcacccttgg tggcgttgta
     1741 ccagacgagg cccggacgcg ggcgcagacc ggcggtcgag ccgatgttca cgatggagcc
     1801 gccgttggtc atatgcggca ccccggcggt gacgaaattg tagatcgact tgacgttgat
     1861 ggcgaagacc ttgtcgaaat cggcttcagg tgtttccaga accgggcggt tggaatagct
     1921 ccagccggcg ttgttgacga cgatgtcgag gcggccgaag gccttcacgg tcgcggcgac
     1981 ggcgtccttc acggcggaca acgaggtgac gtcgcaggct atgggaacag cgttcgcgcc
     2041 gatgtccttc gccactttcg ccgcgaggtc cgccttgatg tcgagaaccg cgaccttcgc
     2101 gccttcccgc gcgtaggtgc gtgcgatctc ctcgccgaag ccttgcgccg cgcccgtgac
     2161 gatcgcgacc ttgccgtgca gtctcatgcc agattctcct gttcgctggc ccaagtctga
     2221 ccgcgaccgg gatttgcggc aagggcggag tcgcgcttga ccgctggccg gcatccttca
     2281 tcatggcagc aatgcttacc taaccgggag ggggcgatgc aggaagccgt gcgccggggc
     2341 gaaacctgga tcgccgcctg gaacagccgc gacctggagc gcatcctcgc gctctacgac
     2401 gatgacgccg tgatggcttc ggagaagatc cggatgctgg ggctggatcc atccgggaca
     2461 ctgcacggca aggagcggct gcgtgcgtac tggcagatcg ggctggcgcg ccggccgggc
     2521 ctgaggttcg acctggtcgc ggtttccgcc tctccggaca gcatcgtggt gcgctaccgc
     2581 gacgagcatg ggcacgaggt ggacgagtac ctgcgatacg caatggacgg gctgattgtg
     2641 cagggatctg ccaatcaccc cgcatgacat ggggcgctac agccagccga ggccagtgag
     2701 accgtcccac atcagcttca gtccggtgat gccgaggaga aacagcacga ggcggaagaa
     2761 cggcttttcc ggaatgaggc cggtgatgcg gaagccgatg aacacgccga tcgcagccgc
     2821 cggcatcaga acgagagcgg tgagcatgat cggctgggtg aactggccga gcgccatgta
     2881 gggcggcacc ttggcaaggt tcaggatggt gaaaaacagc gtcaccgtgg cctgaaacgt
     2941 gcgtttgtcg aggccacgcg gcaccaggta aatctgcacc ggcgggccac cggcatgggc
     3001 ggtgaagctg gtgaagccgg acagtgcccc ccagaacacg ccggccccca gggccggcgg
     3061 ctgcggcgtg tcggctgcgc gcgtcgcgcc ggtcagattg gcggcgacga aggcaaccga
     3121 gatgacgccc atcatcagcc gcatggccgg ttcgctgacc caggaaaagg tgaaggtgcc
     3181 gaacacgacg ccaagcgccg cgccaacgag gagcacccag gtgtgccccc agtgccaccg
     3241 atggcggaac gcccaaatgg cgaagaagtc cgagatgatc agcagcggca gcacgagcgc
     3301 cgcagccgcc cgcgggtcca tcgacagtga caggatgggc accaccaatg tgcccagccc
     3361 gccggacagg ccacccttgg ccatcccgat gatgacggca cagcccaggc cggcaaggag
     3421 catggaggta tcgagcagca tgggctcagc ggatttcgaa cgggccggtt agagcatcga
     3481 acggcgatcc cggcaatgcg ggcgggttgc gttcccagcg tgttgggggc atggtgcgtt
     3541 ctttattccg aacaacgggc gaggggcatg cgcgaggaaa ggcgggaacg cgatctggag
     3601 gcgggggcgg tggccctgtc cggccagctc ggcaagacga tccagcggct gcgcaaggcc
     3661 tacaatctgt cgctgtcgga gctgtccgaa caatcgggcg tggcgaagtc gatcatcagc
     3721 cagatcgaac gcaacgagac caacccgacg ctggcgacga tctggcgttt aagccaggcg
     3781 ctggacgtct cggtcgaccg cttcctcggc caggcggacg atgcgccctt cgtgcagaaa
     3841 tccacggtgc atgacacgcc catcctgatg agcgaggatg gcaagtgcaa gctcgccatc
     3901 atcggctggc tgaagaccgt ggaatggctg caatggtatg acatgcgggc cgagccgggc
     3961 ggcgtgctgg attccgacgc gcatcagcgc ggctccatcg agtgcctgtc ggtgatcgag
     4021 ggcgagatgg aggtggaggt cgccggcgtc accgagaaag cgcgcgcggg cgagacgcta
     4081 cgctatcgat gcgaccggcc gcacaccatc cgcaacgtcg gcccggaccc ggcgcatgcc
     4141 acgatggtgg tgatcctcaa ggccgcggtg atggaatagg gcgcgacgaa cacgcggggc
     4201 ccagggatcg cgtcgggcga cggcaggggc ctatttcaca atcggcagtt tcgcggcgat
     4261 ccagccaatg tccggcgcgc gtccggccat gccgccgcgt acgttgacga tgcccttcca
     4321 gccgcgcttg gcgagttccg actgcacgaa ggcggtgcgg ttggcggtgc ggcagatgat
     4381 gtcgatgtcc ttgccgggat tggccttttg caggctctgg agcttcggca ggaagtcgtc
     4441 ggccgtcatg tcaagcctga ccgcaccgga ggcgacgccg gtttccgccc attcctcggg
     4501 gcggcggatg tcgaccagca cggacttgcc ggaagcgagc cgctcctgcg cctcgctcac
     4561 cgtcaccgag gggagggcgc cctgcgccga ggcgggggcg gcgaaggccg cgacaggcag
     4621 ggcgaggaca gtgcgacgcg ccagcatgat catctctgca tcggaaggcc ggcggccatc
     4681 cagctggacg tgccgccgcg cacgttgacg acattggtcc agcccatgtc gccaagagcc
     4741 cgctggacac tgcccgagcg ggcgccggag cggcagatca ggtcaatcgc ccgcgcggga
     4801 tgacgccgac gcagggcctc aagccgagcg acgaaatcgg gagtgcccat ctggatgcac
     4861 tcggcgccca gggcgacgcc ggtctgcgcc cattcggaag ggccgcgcac atccaccagg
     4921 atggcggcgc cgctttcgac gcgctgcctg gcttcctgca ccgacaccga aggcagcgca
     4981 ggggccggcg tcaacgcgcc ggagagggat ttcatcaggg acgtaaacat aaggcgaatc
     5041 taatataatc ggcgcgcgtt cgcaagtgca aaatgctgcg ctgcggtggt tcgggtcggg
     5101 ccgttccaag gcaagtcgaa atgccgggtg cctttccggg cgctcggcgc atgatcgcgc
     5161 gggtttcacc ccgaggcgct ccggacgagc gaaccagaac ccatccaatc ccttggatta
     5221 caatataaaa aagggattcc aggttcagct gcgcggctcc ggcatggcac cgcgcgcgct
     5281 atttcgcgcc cttcttgcgt ccttcgatgg ccggcagggt ggacaggtat tccgccatgg
     5341 cgttgcggtc ctgcgcggag agttggccgg tgttgccgat tacgggcgtc atgctgccgc
     5401 cgaccgagtc gtagtccggc gtgaagccgg actcgagcag gtaggccagg tccttctttg
     5461 tccagctttt caggccggtc tcgtgcggcg tgatgttggg cacataaccc ttgccttccg
     5521 gatccggccc cccggcgaag cgcttgccgg aggtgatacc gcccagcgga ttgcgcgggg
     5581 agtggcactc ggcgcaatgg cccggacctt ccacgagata ggcgccgcgg ttccactccg
     5641 cgctcttcgc cggatcgggc cggtagacct ggccgtcagc gaaaagcaac ttccagccgc
     5701 cgagcgagcg gcggatgttg aaggggaagg gcagatcatg atcgcggatg cggccctcga
     5761 tgggcggcag cgttttcagg aacgcaaaaa ggtcgcccac atccttcgcc gtcatgcgct
     5821 ggtaggaggt gtagggaaag gaggggtagt agtgtcgccc ctccggcgac acgccttccc
     5881 gcatggcgcg cagaaactgc tcggccgtcc agcccccgat gccgtcacgc ggatgcggcg
     5941 agatgttcgg cacatagaac gtgccgaagg gcgaatgcag cgcatggcca ccgccgagcc
     6001 tggtcttgtc gtcctgcttt tccgtcgcgt ggcacgaggc gcagccgccg gcgtggaaca
     6061 tggtcttgcc gttggccagg tccggcgcgc cgctggcgac gggaagcgcg gaccccttga
     6121 gggcggacag gacggcaggc tcggtgaggg cgaagaaccc ggcaaggcca agcaggccga
     6181 gcatggcgcc gagtttgacg agacgaccga tcatgctgtc acctcatgga aaagaaaggg
     6241 ccgggaaagg gaacgccttt cccggcccgc gatcaaggtc ttggaggtaa ttacttcttg
     6301 atgcgatagg tgttgtggca gccgccgcag ttggccgcca ccttgccgaa ctcggccttg
     6361 aacgaggcct cgtccttcac ggcagccacc gccgcggcgg tgtcggtctc gaacttcttc
     6421 agcgcggcat tccagccggc ggcatccgac cagatcttct cgtcggcggt cgattcgccg
     6481 ccagacttgg ccgtggccgg gaacagcggc ccgaaggcct tcgaggcctt gttcatggtc
     6541 ccgaggctgg ccttggcggt ggcgaggtca aacggacgct cacccttgtt catggccgcc
     6601 atcgggccca tcgcgccgcc aacttccttc atcatcgcct tgcgtgcggc aatggggtcg
     6661 ccctgagcga taccggcggt gacaccgagt gtcagaacgg cggcggcgat gatcatgcgc
     6721 ttcatgaggt ggtctcccgg tggataaaaa ataggcaaat cgacacgcct gcacttgtgc
     6781 acacctaacc cgtcaaatcc atgaacgacg ggacagaatg tcgcaagact ttgcttgcga
     6841 cttcgcaatc tcacaaggat gtgaccaggc cgttatcgcg cctttgccga ccggatggcc
     6901 gcgaacacct tttcgggcgt cgccggcata tcgatgtggc ggatgccgaa gccgcgattg
     6961 agcgcatcca ccacagcatt gatgacggcc gggcaggagc cgatcgcgcc ggcctcgcct
     7021 gcgcccttca cgccgatgct gttggtgacg cagcgcacgt tccgggtctc gaagtgaagc
     7081 gagggaacgt ccgcggcgcg cggcatggcg taatccatga aggacgcggt cagcaactga
     7141 ccttccggcg tgaacacggt gttttccagc agcgcctggc cgatgccctg gatggtgccg
     7201 ccgtgaacct ggccggcaag cagcagggga ttgagcgtga cgccgaagtc atcgacgatg
     7261 acataggaca gcacctgcgt gcttccggtg tcgggatcga tctcgacctc gcagacgtgc
     7321 gtcccgttcg gataggtcgc ctcgggcggc gcgaatgacc ccgtcgtgtt cagcaattcg
     7381 gcggtcgcct tcctcgaacg ggctatgccg gcgaaatcga tggcgcggtc cgtgccggcg
     7441 acgcgcaccc gtccgtcggc tatctccagg tcggaggggg ccgattccag ttcgtccgca
     7501 gcgatcttgc gaaggttttc agccagcctg gcggccgcga catccaccgc cgcacctccg
     7561 accggaatcg agcgcgaccc gcccgttccg ctgccggtcg gcgtctggtt ggtatcaccc
     7621 tgcagcacgc ggattttcga cagcggcaga tcgagatgct ggctggcaag ctgggcatag
     7681 gccgtttcat ggccctgtcc gttggactgc gagccgatgc ggatggtgat gctgccgtcc
     7741 ttttcgagcg cgacgtaggc cggttcagct ccaccgccgc cgcacgcctc gatgtaggtg
     7801 gcaagcccga tgccacgcag cctgccgcgc ttcttcgagg cggcggcgcg cttgcggaag
     7861 ccggaccagt ccgacacctc catcgcgcgg cgcatgacgc cctcgaagtc gccggaatca
     7921 taggtgcggc cgaccggcgt ggcatatggc atccgcccgg gcctgatgta gttgatcgag
     7981 cggatgcggt ccggcgtgcg gccggtttcc agcgctatgg cgtccaccag ccgttcgatc
     8041 agataggcgg cctcgggccg gccggcgccg cgataggcat cgaccggcac ggtgtgggtg
     8101 tagatgccgc gcacgcggaa atgggcggcc ggtatgtcat agacgccggt cgtcatcgta
     8161 ccgcccagga aggggatgta cggcgcatac tgcgacaggt aggcgccgaa atcggccaga
     8221 aggtccacgc gcatcgccag aaaccggccg gagctcgaca gcgccatctc tgccgtggcg
     8281 atgttgtcgc gcccctgcgc gcaggtcagg aaatgttcgc tgcgctcctg cgtccaggtc
     8341 accggcttct tcagcttgcg cgccgcgacg gcgagcagcg gatactcccg gtacatgaag
     8401 gccttgttgc cgaaaccacc accaacgtcc ggcgtgacga cacggatatt ctcagacttt
     8461 tcgccgagga tcttcgccag cgtgtcacgg ataccatggc tgccctggct gccgagcgtc
     8521 agtgtgtagc gtttcttcgc ggtgtcgtat tccgccgtgc agacgcgcgg ctccatgtag
     8581 ttcgccacga ggcggttgtt gacgatcgtc agcttcacga cccgatccgc cctggcgaag
     8641 gcggcatcgg tcttcgcctt gtcgccgata tggctgtcga gggcgatgtt gccggtcacc
     8701 tcggcccaga ccgccggcgc gccctttttc atcgcggcct tgatgccgac cgccgcgggc
     8761 attgccttcc agtcgatctc aagagcctcg gcggcgtctt tcgcctgcgc cagcgacttc
     8821 gccaccacca tcgcgacggt atcgccgacg tggcgcacct cgccctgcgc cagcgcggca
     8881 tagggtgtgc ggaccatgga cgagccgtcc gaattcttca ggatggcctg gcagggaatg
     8941 ggattgaggc cggcgacatc ctcgggggtg aggacgagga gcacaccttt caaggcgcgc
     9001 acggcttcga cgttgcggat ggcgaaggtc gcgtgcgcat gggggctgcg cacgaacgcg
     9061 gcataggtca cgccgtcggg ccgatagtcg gtggcataat ggcccgagcc ggtaatgaac
     9121 cgctggtcct cgacccggcg gaccgcctgc cccatgccga acttcatcgg tttcatcgtt
     9181 cgcttcccct cgtgactttc gctctttcat accaagggtc acgagaaatg acccgtggtg
     9241 cctgtttcga attttcattt cgcaactgaa aaaacgaaaa ttcgaaagtc gtcatcgccc
     9301 acgtttcgtg accgatggtg tcaggaccga tggtctcagg cccccgaatc gaccggtccg
     9361 ccggccatga tccagtcctt gaagccgccg cgatagtgtt cttctatccc gagccccgcc
     9421 gactgcgcgg ccatcagcgc atgctggctg cgtacgccgg cggcgcagga gaacacgacg
     9481 cgcctgccgt cgaactggcg cagccagttc acgtcgaaac gcgacagcgg catcgacagc
     9541 gagccgggaa ttcggcccat ggcgaattca ttgtcttcgc gcacgtccac aaggatcagg
     9601 gttccgtcgg caaggccctg ccgcacgtcg tcaagcgaaa ggtcgatgat ggtcatggtc
     9661 cagaaagggt ttggagcatc aaggctcccg gtacgtggga gcctattacc aaatctcaag
     9721 gagggaaacc ctccaggaaa gggttcgtcg ccccgccggt aacgtgggcg cggtgtcgtg
     9781 ccgttcaata ctcgaagctc tggccttcct tcacgaagaa ggcctccgcc atcttggagc
     9841 ggatcttgcc gtccagcttg gcgagccgac gatcgttgaa ctgcggattg tgatggaagg
     9901 cagccaactg cccgaccttg gcgctctggg cgagcttgac gccggccttc caggtggagt
     9961 ggccccagcc gcggcaggac acaagctcgt cctcggtcag catggtgtcg tagatcagca
    10021 ggtccgagtt cctgacgaac cggcgcacgt cgggatcggg agcatcctcg acgtgttcga
    10081 cgtcggtgac atagctgacc gacttgccgg cgcgctcgaa gcgatagccg gtgctgccgc
    10141 cgggatggtt caggggctgt gttgtcaccg tgacatcgcc gaaggtgaag gtttcgccgg
    10201 ccaggaaacc ttcactgcgg attttcgcag caaaacggct gaagacgatg gggaagaacg
    10261 gcgggccgaa aacctggtcg aggtgtttct gggcgctttc gccgcccaga ttgccacagt
    10321 acatggtgat tgacacgctc ttgtcatgga tcggagcgaa aaacggcagg ccggccgtgt
    10381 gatcgtggtg caggtgtgag aagagcagga tgatgtcctt gacaccttct tcctgcagct
    10441 tgcggccgag cgccaccgcg ccggtgccgg catccagcac gaagacctgc ttgccggcgc
    10501 gaatttccac gcaaggcgtg ttgccgccga attccatgta ttcggccgag gccgtcggca
    10561 cgctgccgcg gacaccccag aacgttaccg atagtgctgt cacatccgcc cccggatgcg
    10621 gggaaatgac cccgcgccgt tatcaagata aacaataagg ttatggaaag gctgacatcc
    10681 gagatgtgcg ccagcgcaca tggcgcgaca gtcaggccgc cgtcgatgca tagttcctca
    10741 tcatgatcgg ctggcgcttg cgcccccatg tgccgcgcgg accgtcaaac agccccgcca
    10801 tgggggtccc gcaatcaagg cagcgccctc cctcgtcgag ccggtaatcg gtgataaggt
    10861 gccagtcacg ggcgatgacg cgcctgccgc agccggtgca ataggtcgac tggcgattga
    10921 gatcgtgaac gttgccgacg tagacgtgct tcaggccttc cgagatcgcg atggcgcggg
    10981 catgcgccag cgtccggtgc ggcgtccttt ccttgtcgag cattcggtgg tccgggtgga
    11041 aggccgagaa atgcaaggga acgtccggcc gcagcgcggt ggcgacccag cgcgacagcg
    11101 cccggatttc ctgttcggaa tcgttctccc cggggatcag caaggtggtc agttcgagcc
    11161 agaccttcgt ttccgcgacc agccagacca gagtgtccat tacgtccgcg aggctcgatc
    11221 tggtgtgctg tcggtagaag tcctcggaga aggccttgag gtcgacgttc gcggcatcca
    11281 tggcgccgaa gaactcgccg cgcgcctgtt tcgaaatgta accggccgtg accgcaacgg
    11341 tcctgatccc gaccgcccgg caggcggccg cgacatcgac cgcgtattcc aggaagatga
    11401 ccgggtcgtt gtaggtgaag gcgacggagc ggcagccatg ggcgagagcc gcccgcgcga
    11461 tggcctcggg cgaggcctcc tgcgccaggg tatccatttc ccggctcttg gaaatctcgt
    11521 ggttctggca gaaggaacag gcgagattgc agccggccgt gccgaaggag aggattggcg
    11581 tgccgggcag gaaatgggcc agcggcttct tctccaccgg gtcgacgcag aacccggacg
    11641 agcggccgta ggtggtcagt tccatcgcct cgccgacgcg ctggcgcacg aaacacaggc
    11701 cgcgctggcc gtcattgagt ttgcacaggc gcggacagag cgtgcactcg acgcgcccat
    11761 ccgtcagctt gcggaaatac cgtccgatca cgcgtctgtt gctcccaacc tgtcgccgac
    11821 tgacgcagca tcatgaccgt tcatggcttt tcaacttagt tttgcttggt taagcgccga
    11881 ttgacgatca gaggcaccaa tgtccgctcc ttccatcagc cgcgccgcgc cgattgaact
    11941 ggacgttctt cccgcccagg tcgccggcac cttctatccg gaggacaggc agggcctgtc
    12001 caaccagctc agccgctgca tcgtcagcgc cgtccagtcg tcgatccggg cgcccaagat
    12061 aatcatcgcg ccccatgccg gcttcacgtt ctcgggcggt gtcgccgctt cggcattcca
    12121 tgggctggcg aaccggtccg acctgatccg ccgcgtggtc cttgtcggac cggcgcacaa
    12181 gatgagcttc aagggcatcg ccctgcaccc ggcccgagca tggcgcacgc cgctcggcga
    12241 tgtgacggtc gactggacca tgctgagacg cgtgcagccg ctgcctgggg tcgtgatgga
    12301 cgacaggccc ttcctgcgcg aacacgcgct ggaaacccat ctgccgttcc tgcaggtgag
    12361 cctgaagcag ttcgaaatct gtccgatgct ggtcggcgac gcttcgcccg agctggtggc
    12421 ccaggcgctg gaacgggtct ggggcgggcc ggagacgctg atctcggtct cctcggacct
    12481 gtcgcatttc ctttcggaca cggcggcccg gaccatcgac caggagacac gccggatcat
    12541 cgagacgctg gacgccgagc ggctgaacgc gacacgggcc tgcggccatc gcgccattgc
    12601 cggtgcgctg cgactggccc ggacgcacga tctgcgggtc accggcatcg acctgaagaa
    12661 ttccagcgac atgacggatg agcgcgagcg cgtggtggga tacggcgcct tcgcgatgga
    12721 gtatgcggcg caggccgaac tcgcgccggt cgagcgcaag ctcctgctgt cgacggcggc
    12781 gcgctcgctc gtgcacgcct gccagaacaa cggcgcgacg ccggacgtcg ccatcaacgg
    12841 caccctgccg gcgacgctct cggccatgcg cgcgacgttt gtttctctgg agtccggtgg
    12901 gcgccttcgg ggttgcatcg gatccctgaa gccacaccgc ccgctgctgc tcgacgtgat
    12961 cgccaacacg gtcaaggccg gattcgccga tccgcggttt ccgaagctga cgctccagga
    13021 aatgagcggc ctgaccctgt cgatcgcgat cctgtcgcat ccccgcgaga tggaattcga
    13081 cagcgaatcg gcactgatca aggatctttc gcccgaccgc gacgggctca tcatcgagga
    13141 tcagggccga agcgcgctgt tcctgccaca ggtctgggcc aacattcccg atcccaccgt
    13201 cttcctgaag gcgctgaagc agaaggccgg cctgccggcc gaccattggt cgcccacctt
    13261 caaggccaga cgtttcgcgg cggaaaagtt cggccggccg atcgtcgaca tcctgaaagg
    13321 atgatgcgcg cccgcttcgc tacagcggcc ggcccggtcc ggtccggcgc atgcgcttgc
    13381 gctcgatgcc gagctggcgt tcgcgcagga tgacgaagac gcccgaggcg atgacgatga
    13441 ccgctccgat cagcaccagc ttctccggga tctcgccgaa cagcagccag ccgatcagca
    13501 gcgcccagat catgctggtg tagtcgaacg gggcgatgac cgacgcatcc gcgtagcgat
    13561 agctgtgggt catcaggatc tgcccgatgc ctcccagcat gccgatggcg gccagcatcc
    13621 agaaatcgcg cgctcccggc caatgccagg ccatctccgg ccggacaaga ccgaggccga
    13681 aggtcacgag cgccgcgacc gtggtgaaca acgaaaagta gacgacgatc gcggcggtgt
    13741 gctcggtcgc cgtcagccgc cggacctcga tggtcgcgac ggcggcacag agcgcgccgc
    13801 caagcgccag ccagacaccg gtcgcggcgc cgggcgggac gtgcttgccg ccgagcgcca
    13861 ggtgcggcag cagcatcacg atgacgccga acagcccgat gaccacagca gtccagcggt
    13921 agaggcgcac ggtttccttg aggatcacgg ccgcgagcag gaccgaaagc agaggcgcgg
    13981 cataactgat ggccgtcgcg tcgggcaggg gcagatacac cagtccggca aagcccagga
    14041 acatgccgag tgtgccaatg atcccgcgac ggatatggcc cttgaggttc ttggtggcga
    14101 aggcggccga cagcggccga gcatacgtaa gccagatcaa caccggaaca agggcgaaga
    14161 acgagcgaaa aaagacgagc tgaccgaccg gtgggttgaa cctgatgccg cctgtggcgg
    14221 agtgtcgaac gatggccgcc atcagggtga acgacagggc ggacagcacc ttgagcagga
    14281 tgcccaagat cgggcgtggc cgccgcggcg catcggcagg gtccgggctc gcgggcaatg
    14341 gggtgtcaga cacagtcatt gatcgtcttg atgatcaccg ccagcacgcg gtccccggtc
    14401 ctaacgtgga tcgggccacg ataggggttc agcggcgctc tgcaaccgcc gtctaccagc
    14461 cgctcaggag aaaaggcgaa ggcgttccgc cgttggcagg tcgtcgagag gctgcatcgc
    14521 gcctgtccgg gccggtgccg aagtctgccg gggctgaggc tcgccggccg gcggttcggc
    14581 atcgggcagt tccagcatcc tgtcgatgcg ctcctgccgg cccgcgtcat cgggccagaa
    14641 cgaggcgggc tggccggtgg gttccgaggc ggcggcgggc gattccacat tccaggcctg
    14701 catgatctgc ttgatacggc cttcgacgta attcagcgtc tcgatgacct tctgggtccg
    14761 ttgcgccgtg atgtcctgga atccacaggc ggcatagatc tcggtggcgc aggcatcgag
    14821 gttgtcgcag tgtgcctcgg gcgcgcccag ttcgcgcatc gtccaggcct gctgctggac
    14881 ctgctcggcc gccgacagga tgctcgatgt ggcttcctcg gtcgccctga caatggcatt
    14941 gagttcgccg ctggccacgt taaggcgggt cgccgccagg ccctgaggac ggatccgtcc
    15001 gatctcgacg tgggtgatgg cgatggcgcg cgccatttcc accaattcca ggcgcatgcg
    15061 ttcgagccgc gcctcggtgc gctggcgttg caggttgtgg tgcagcttct cgatggcccc
    15121 gagtacgcgt tcggtgtcgg cgtgccggtt gcggcgcgca tattccgaca gaaaccagcg
    15181 gccgcgctcg gtctccatga ccgcggcctc gatggtgtcg tagtccgcct ggctgatcgg
    15241 cgcaggcaat ttcccatgtg tcatcgcgcg cactcgaacc ggcgctcagc cggcctttgc
    15301 gaccatcatg cctacgagac ggttaacgcc gcgtgcatgg cgcgaaagcc acagagtggg
    15361 cggcgcgggc gaaacgggac cactttgcgc cgtgccacgg gttgccgctg ggcatcacac
    15421 aggacattat gcgcgaaact tcataggcat ttgttccgaa gtggctgtca agaagcgcct
    15481 gaccctggag ccggtcatga cgatccaacg cgcgggcgat ggagcggcgc tggcgtttgg
    15541 cggcgactgg tcggcctcca acgcgcatga gtgcgaagcg gtgctggagt cggccaccgg
    15601 tcgaatcggt ccggtcaagt gggttcagat cgatgtcggc ggcatcggca ggctggatac
    15661 gctgggtgcc tgggtgatct cgggcctgcg ccaccagctc gagtccgccg gcgccaaggt
    15721 gagccttgtc gaagcgcgca cgccgcaccg catcctgttc gaggaagtgt cgtatcgccc
    15781 ccttcctgaa tcgcccaagc gccgggagtt catcgccgtc gacatgctgg ctgatgtcgg
    15841 acgggcgatg gacaacgcgt ttcaggacct gatcaacggc atcgccttcc tcggcgcgtt
    15901 catgagcggc ttcgcgaatg tcatcctagg gcgcagccgg ttccggatcg cctcgctggt
    15961 ggcgcagctg gagcgcgtcg gcctgcggtc ggtgccgatc atcgtgctga tctccgttct
    16021 ggtcggcggc atcatcgcgc agcagtcggt gttccagctt cagcagtttg gaacaacgca
    16081 tttcgtcgcc gatctgctcg gcgtgctggt gttgcgcgaa cttgccgtac tcatcacggc
    16141 catcatgatc gccgggcgca cgggatcgag tttcaccgcc gaactcggct ccatgaagat
    16201 gcgcgaggag atcgacgcgc tgaagaccat gggcctcgac cccatcgaga tccttgtggt
    16261 gccgcgcatt ctcgcgctgc tgatcgggct gccgttgctg accttcatca gcgaaatggc
    16321 gatgctgttc ggagggggcg ttgtggcgat ggtctatggc ggcgtcagcc cggacgtgtt
    16381 cctgtcgcgc ctgcagaaca tcatcggcct caacacgttc gtcgtcggca tggtcaaggc
    16441 accgttcatg gccctggtcg tcggcgtcat cgcctgcctg gaagggatgt ctgtgggcgg
    16501 gtcagccgaa tcgctcggcg cgcgcaccac gtcctcggtg gtgaaggcca tcttcatggt
    16561 catcgtcgtc gacggcctgt tcgccatgtt cttcgccgcg atcaagtatt gagccggcga
    16621 tgagcgagca cgccgagccg gacgaaaagc gcgacaaggt catccgggtg cgcaacctga
    16681 cagtcggctt tggcgagaag ctgatcatga aagggctcaa cctcgacgtc tatcgcggcg
    16741 agattctcgg attcgtcggc gcgtccggta ccggaaaatc ggtgctgatg cgcaccatcc
    16801 tcggccttgt gcgtcagcgc gagggcaaca tcatcgtctt cgacaaggac ctcgatacgc
    16861 tcacccagcg ccgccgcgag gcggtggaac ggcgctgggg cgtgcttttc cagcacggcg
    16921 cgctgttttc ctcgctcacc gtgcgtcaga acatccagat gcccatgcgc gagtatctca
    16981 gcatgtccgg ccggcttctc gacgaaatgg ccgcgctcaa gatcgagctg gtcggcttga
    17041 agcccgatgc cgccgacaaa tacccgtccg agctctccgg aggcatgatc aagcgcgcgg
    17101 cgctggcccg cgccctggcg ctggacccgg aaatcgtgtt cctggacgag ccgacgtcgg
    17161 gcctcgatcc catcggcgcg caggaattcg atgatctcat cgccacgttg cagcgtactt
    17221 tgggcctcac ggttttcatg gtaacgcacg atttggacag tctggcgggt gtttgcgacc
    17281 ggatcgcggc tcttgcggag ggtaaggtga ttgcggcggg gccgatcgaa acgatgatgg
    17341 agtcggacca tccctggcta aaggcctatt tccatggctc gcgcgcccgg cgcggcagcc
    17401 tggaaacggg ccgattccgt gcaagcctga ggtcctgatg gaaacccgcg ccaattatgc
    17461 tctggtcggt ctgttcaccc ttgcggtgat cgccgcaagt ttcgggttca tctactggtt
    17521 ccgttctgcc agcaatgccg gcgagcgcgc gccctaccga atcgtgttca acggttcggt
    17581 gtcggggctc tcgaagggcg cgcaagtgcg cttcaacggc ctcagggtcg gcgaggtcat
    17641 ggccgtcgaa ctgatgccag acgatccgag ccgcgtctcc gtgctggtgg cgatcgatcc
    17701 caaggtgccg atgcggaccg acacgcgcgc ccggctcgat gtgcagacgc tgaccggagc
    17761 ggcat
//
LOCUS       DAOMXN010000029        17401 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_1861_length_17401_cov_10.930704, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000029 DAOMXN010000000
VERSION     DAOMXN010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 17401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17401
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1861_length_17401_cov_10.930704"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="PK812_04295"
     CDS             <1..967
                     /locus_tag="PK812_04295"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF036802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HRJ68808.1"
                     /translation="FASNVARMRSVAEAALAAGRQVIVAGRAMERVTEVAGELGMLEG
                     LKPFRGPDVFPRLARDNVVVLVTGSQGEPRAALARIAANEYPDIRLASGDKVIFSSRA
                     IPGNEKGINAIINALAKQGIDIVTDRDALVHVSGHPRRDEVRQMYEWIRPKVAIPAHG
                     EALHLTEQAKLARSLGVEHVIRGFNGDMIRLAPGDPVVTGQAPSGRLVKDGDLVTAVD
                     GAVQERRKLSFAGIVTVALALDERGEIVADPEIETTGIPDKAANGADIAELALDAVES
                     AFGSLSKAKRRDPATVQEAVRRAVRAAIADVWDKKPICQVFVLEV"
     gene            995..1399
                     /gene="mce"
                     /locus_tag="PK812_04300"
     CDS             995..1399
                     /gene="mce"
                     /locus_tag="PK812_04300"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HRJ68809.1"
                     /translation="MIGRLNHVAIAVKNLEAAATTYRSALGARVSAPQALPEHGVTVV
                     FVELPNTKIELLEPLGADSPIARFLEKNADGGMHHVCYEVDDILAARDRLKAGGARVL
                     GDGNPRIGAHGKPVLFLHPKDFNGTLVELEQV"
     gene            1403..1630
                     /locus_tag="PK812_04305"
     CDS             1403..1630
                     /locus_tag="PK812_04305"
                     /inference="COORDINATES: protein motif:HMM:NF018978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1467 family protein"
                     /protein_id="HRJ68810.1"
                     /translation="MKVTTWFAVYFVIWWLTLFTVLPFAARRKLEVEPVAGADPGAPP
                     KPNFVAIMLATTLVAAVVFAIVWWFIENGYV"
     gene            complement(1608..1853)
                     /locus_tag="PK812_04310"
     CDS             complement(1608..1853)
                     /locus_tag="PK812_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68811.1"
                     /translation="MIASEPDNMRTLGFAVLWFFAGLVITWVATVAAGVAWMTATGFI
                     DRDGGASMGLVFMIGPTLGVLGGLIAAIWSVIRSRSR"
     gene            complement(1873..2085)
                     /locus_tag="PK812_04315"
     CDS             complement(1873..2085)
                     /locus_tag="PK812_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HRJ68812.1"
                     /translation="METRDSNGAVLKDGDNVTLIKDLKVKGTSVTLKRGTLVKGIRLT
                     GDPDEVECRVEKVKGLVLRTEFLKKA"
     gene            complement(2093..2650)
                     /locus_tag="PK812_04320"
     CDS             complement(2093..2650)
                     /locus_tag="PK812_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HRJ68813.1"
                     /translation="MHKPDVSDFAGLRPLPRLVFGSRWLQLPLYLGLIVAQCVYVFLF
                     LKELVHLVGDAYKLTEQQIMLIVLGLIDVVMISNLLVMVIVGGYETFVSRLHLKGHPD
                     QPEWLDHVNASVLKIKLALAIIGISSIHLLRTFIEAGNLGKPNSNYTEAGIMWQTIIH
                     VIFILSAIGIAWVDKIQHPTASKPH"
     gene            complement(2709..4454)
                     /locus_tag="PK812_04325"
     CDS             complement(2709..4454)
                     /locus_tag="PK812_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRJ68814.1"
                     /translation="MSDISFESSPIRSQASRDTWRAWPARHGIPARWAWSIVTIIAVV
                     LAFNYVRTEGRWPTQLIAFGGTISFAALLAFATRRVLFATFLTVAIIVTIKLAAIAKL
                     QTMNMVLHAYDLVFYLSDWPTVKYLLTEFSDQFLLLAFVLLTVAGLGWVIFRADPTRA
                     RRSLSAAVAVAAFAVSVAGVIAMGERRHQQFEYQGQFISSFLISWQETLETLWKGQLI
                     DAAHAAAGPPLAPLERCNTAFKPPHIILIHQESVFPPSVFANLNYDRSMDPFFRSHDG
                     QLHKLRVETYGGASNLTEFSILTGLSTRSFGGMRQFLQTVMAGKIREALPHVLERCGY
                     RNVMFYPMLKNFAQTGRFFEGVGLTDIRDKRGQNAPTFNERDAFYYRSALDEMERHFA
                     QSRQPLFTFIETMSAHWPFNFTYAPEMEVPGGLEGTHPEMHEFLRRVSIAKIDYDKLK
                     KELARRFPSERFLVVMYGDHQPMSTRMLLNFAENSDSEDVVIPPDSIGFHTFYAVNGV
                     NYAPPPLPRHELVDVAYLSTLLLESARLPLPDSYRERRRLLDVCKGRYSDCVHQQEVL
                     AFHRRLIDANLMQAR"
     gene            complement(4580..5731)
                     /gene="aliB"
                     /locus_tag="PK812_04330"
     CDS             complement(4580..5731)
                     /gene="aliB"
                     /locus_tag="PK812_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclohexanecarboxyl-CoA dehydrogenase"
                     /protein_id="HRJ68815.1"
                     /translation="MNPYIDADLQALAEQARKFAEARVAPGFQERDRTRVLDRALMRE
                     MGAMGFIAPELPEASGGLGLTCTAAGIIHEEIARADLSLSYINLLASLNGQILSRYGR
                     AEVTRPWLDRILAGEALCAIAVTEPKGGSDAGAMRLKMERDGAHFVLNGEKTSISAAD
                     QADVVVVFARSGRIEDGARGISALLVPTDLAGLTRSRFDCHGQRAIGRGSLFFDGVRV
                     PADHLLGNEGEGFVQVMQGFDFSRALIGLQVLAVARAALDEAWAYASQREAFGKPISA
                     FQGVSHPLADFDTQVSAARLLCFQALWLKDRGLPHSAEAAMCKWWAPKLAYDAIHAAL
                     LTFGHGGYDRGVMEQRLRDVLGFQIGDGTAQIMKTIIARHRAGRKAVPA"
     gene            complement(5738..6520)
                     /gene="badI"
                     /locus_tag="PK812_04335"
     CDS             complement(5738..6520)
                     /gene="badI"
                     /locus_tag="PK812_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketocyclohexanecarboxyl-CoA hydrolase"
                     /protein_id="HRJ68816.1"
                     /translation="MTYEDILYEVRGPAAWIIINRPDKMNAFRGKTCDELIHALNRAG
                     YDKSIAAIVLAGAGDRAFCTGGDQSAHAGAYDGRGTIGLPMEELHAAIRDVPKPVIAR
                     VQGYAIGGGNVLCTICDFTIASQKAQFGQVGPKVGSVDPGYGTAFLARVVGEKRAREI
                     WYLCRRYTADEALAMGLVNAVVAPDQLDAEVQKWCEEIAERSPTAIAIAKRSFNMDTA
                     HQAGIAGMGMYALKLYYETEESKEGVRAFNEKRKPDFRKHQK"
     gene            complement(6544..7311)
                     /gene="badH"
                     /locus_tag="PK812_04340"
     CDS             complement(6544..7311)
                     /gene="badH"
                     /locus_tag="PK812_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycyclohexanecarboxyl-CoA dehydrogenase"
                     /protein_id="HRJ68817.1"
                     /translation="MQGLSGASVIVTGGGGGIGGATCRRFAAEGAKVAILDLNLAAAE
                     KVAADITAAGGTATPLACDITDYEQVASAVAAAEAALGPVQVLVNNAGWDVFKPFTRT
                     APQDWPKLIAINLTGALNLHHAVLPGMVARKAGRIVNIASDAARVGSSGEAVYAACKG
                     GIVSFSKTIAREHARHGITCNVVCPGPTDTALFEGYKQGAGNPEKLVEAFERSIPLGR
                     IGRPDDLPGAILFFASDDAAYITGQVLSVSGGLTMVG"
     gene            7397..7894
                     /locus_tag="PK812_04345"
     CDS             7397..7894
                     /locus_tag="PK812_04345"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRJ68818.1"
                     /translation="MQNERVLAPGARKADDADLRHDLANRLFFRLYQASNLLHKVGTK
                     AVSGFGATTQQWAVLGALARQGNERQGLSIKQLMEYLLTSRQNLTLVLDRLEGQGLVE
                     RVRGTTDARVRHVRLTAQGRKTWEEMLVDIRAFYGQATEGFSTEEVFLLFRLLDRLKD
                     RLSAL"
     gene            complement(7883..8848)
                     /locus_tag="PK812_04350"
     CDS             complement(7883..8848)
                     /locus_tag="PK812_04350"
                     /inference="COORDINATES: protein motif:HMM:NF013885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68819.1"
                     /translation="MKSLASALAYIGRHGTEGFALALFMGLALPQFAERARPLLPVTI
                     FCFMVIVFLRADITVIAGLVRRPAKLALSCLWLVCMPALIAMLAILAVGRESIDPGLL
                     LGLAILGAAPPIMSSPAVAILYGFEPSLIIACVLGITVLSPLVSPLLVDWIAGQAVPL
                     DQSVLMMRMAVFVGGGFLTAMALRRWLGTPRVAELKAEFDGFGVVMYFLFAIAAMDGV
                     TEAAFREPARVAGFLGAALAVTALGLVLAMALLRFANPAERFILGYGTGQRNMGLLIA
                     ALGAGVPDSTYLFFALAQVPIYLMPWLLKSVARRIRDRQQAASQG"
     gene            9046..9978
                     /locus_tag="PK812_04355"
     CDS             9046..9978
                     /locus_tag="PK812_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRJ68820.1"
                     /translation="MKRLLLAAASVAALAAPAAAQDIRMMTGPQGGIWVPLGGQLKDL
                     WEKAVPGLKVATLPGAGVANARAIEEAKAEVGFGNTITTADAVEGKPPFDKKHGKLCN
                     VASLYPQYFQAIVLADSGINSVKDIKGKTITTQQRGNTGELITQHYLKAHGLSYSDVK
                     VNFGSYTESVEQMKDKQAHLFTLGAGIPLGAVMDLAAGRDIKLLDQTEAHAEMKKMNP
                     GYVLLPVPKGTYPKQEQEVKVIAYATHVVASCALPADTVYTMTKAIAENTQLLGNLNK
                     DMGKLTPKDMAENVGVPFHPGAARFYKEKGITVN"
     gene            10037..11932
                     /locus_tag="PK812_04360"
     CDS             10037..11932
                     /locus_tag="PK812_04360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HRJ68821.1"
                     /translation="MTNAARRASVWKTVIGVIACVMALYHLWVAWFGAPEQHLFRGQH
                     LLFALVLTFLWYRRTTPTEDELAMRLEQGGFIPPHWSDLALVVLSAVPLLHLFWNYGY
                     FVNRIYYVDDLSLFDQACAIVLMLLILEGARRLMGWALPLTALVFLVYALAIARVEPM
                     RMLDQLYMTTEGIFGMSLGVSASYVMIFVLFGAFMERTGTGQLFMDFAMSITGHTAGG
                     PGKVSVVSSSLFGTISGSAVANVMVDGPISIPLMKRTGFKPHFAAAVEAVASTGGQIM
                     PPIMGAAAFVMAEFIGVSYGQVVIWAAIPAILFYVACFASVHFEAKRTGLHGVPRSEL
                     PKLGATLAERGHLFLPVLTILAVMYAGYSAPLAALAGTLACFPVAALRASTRSNVSVK
                     NVVEALIDGAKNTLAVALACACAGIIIGIVTLTGLGITFTQFVVGLSKDTLLLALLLT
                     MIAGIVLGMGMPTTPAYIIMAALLIPAIIKLGVPKPAAHMFAFYFAILSAITPPVALA
                     VYAAAGLAKSDIWKTGWSAVKVGAAGFIVPFMFVYEPAILMIGTWPDIIHATVSAILG
                     CLLLAAGLAGYWLAPANAAQRLLLIAGAFGLVTPGVISDLAGAGIAAVVTLWQLAGRK
                     KAQEAGP"
     gene            complement(11971..12870)
                     /locus_tag="PK812_04365"
     CDS             complement(11971..12870)
                     /locus_tag="PK812_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HRJ68822.1"
                     /translation="MPTKDLVRLVKKYRVRDGRGFDLDDYETENPLGDAMDEKAEARS
                     VLDEGIKELSEIQERLFAQDEWSVLLIFQAPDAAGKDSTIKHVMSGINPQGCHVAAFK
                     RPSDEELDHDYLWRCVRQLPERGRIGIFNRSYYEEVLVVRVHPEILAKQHLPQDLKED
                     PDIYDHRLEDIRQFERYLGRNGTKVIKFFLNVSKDEQKRRLIERIDEPEKNWKFELND
                     LNERALWDEYRVAYTNAIRQTASKRAPWYVIPADDKWFMRVAVVTAILHELRGLDIKY
                     PEISDKERAELAQAKKRLMREKD"
     gene            12923..13627
                     /locus_tag="PK812_04370"
     CDS             12923..13627
                     /locus_tag="PK812_04370"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRJ68823.1"
                     /translation="MRGSLASGRRIGITGGMLIIYDCDGTLIDSEGIACSVCAEALSG
                     LGVPYTTEMFAARYAGRPARETWDHVIATYGVTLPDGFNARINAEIHARLDAGVEAVE
                     GARAAVEALAGPRCVASSTGLTQLRKNLATAGLIDLFGPHVFSVSQVKRGKPAPDVFL
                     YAVSQMGFDPADAVVIEDTVPGVTAARRAGIRAIGFTGANHGGDGLGDRLRQAGALDV
                     VAAMADLPGVVASLRR"
     gene            13685..14182
                     /gene="coaD"
                     /locus_tag="PK812_04375"
     CDS             13685..14182
                     /gene="coaD"
                     /locus_tag="PK812_04375"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012319124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HRJ68824.1"
                     /translation="MTSGFYPGSFDPLTNGHIDVLKSCFPLVDRLVIAVGIHPGKAPL
                     FSAEERLEMLKVCCGPLAQQAGVRLETTTFGDLTVSAARRSGCNIIIRGLRDGTDLDY
                     EMQLAGMNQTMAPEIHTVFVPASPLARPITATLVRQISAMGGDVSGFVPPYVLEQLRL
                     KSAPR"
     gene            14209..14760
                     /locus_tag="PK812_04380"
     CDS             14209..14760
                     /locus_tag="PK812_04380"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRJ68825.1"
                     /translation="MLRRTLFAAALALLPVAALAQQSRQDAENTIHLELKDGRVVIRL
                     RSDLAPKHAEQIKTLARRGFYDGLTFHRVIADFMAQTGDPNGNGTGSSNLPNLPAEFS
                     REPFKRGTVGMARASDPNSANSQFFIMFKEGAFLNGQYTVVGEVIQGMDAVDKIKKGD
                     VNRNGAVSQPDKIIRMSVAADSR"
     gene            14782..15252
                     /locus_tag="PK812_04385"
     CDS             14782..15252
                     /locus_tag="PK812_04385"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRJ68826.1"
                     /translation="MTTAAEDTLIFETTKGKVVIEMRPDLAPGHVAHIKKLVREGFYD
                     GLTFHRVIDGFMAQGGCPKGTGTGGSKYPDLKAEFNNAPHVRGVCSMARAQNPNSANS
                     QFFICFADARFLDRQYTVWGKVIEGMDNVDQFARGEPVRNPDKIIKATIAAEAA"
     gene            15277..16362
                     /gene="queA"
                     /locus_tag="PK812_04390"
     CDS             15277..16362
                     /gene="queA"
                     /locus_tag="PK812_04390"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008598435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HRJ68827.1"
                     /translation="MRLSDFDFHLPPERIALHPARPRDAARMLVVRPDHVPEFQDRIV
                     SDLTGFLRPGDALVFNDTRVIPARLFGRRDRDGASVSVEALLHRRLDASRWLAFARPG
                     KRLRVGDRVSFGDADGNACLLGALHGTVEEKHEGGEVAIAFDLSGPALDDAIAAAGEM
                     PLPPYIASARPAGIQDRTDYQTIYAREAGAVAAPTAGLHFTQDLFARLDAMGVSRHFV
                     TLHVGAGTFLPVKAETLVEHKMHAEFGSVSAACSTMLGEVRSKGGRIVAVGTTTLRLL
                     ESAAAETGTIRPFAGETDIFIKPGFAFRAVDLLLTNFHLPKSTLFVLVSAFAGQERMR
                     RAYAHAIAGGYRFYSYGDACLLERAGG"
     gene            complement(16520..>17401)
                     /locus_tag="PK812_04395"
     CDS             complement(16520..>17401)
                     /locus_tag="PK812_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ68828.1"
                     /translation="IGDVRRAQGDLTGALTAYAASHANFERLAAANPSNTQRQGDLSI
                     SHDKIGDVRTAQGNLAGALMAYEASLAIRQRLAAADPSNTVWQRDLSVSQNQIGYVRV
                     AQGDLAGALTAYEASHAIFERLAAADTRNTQWQRDLSISHDRIGDVRRAQGDLAGALM
                     AYEASLAIRQRLAAADPSNTQWQRDLSISQEKIGDVRVAQGDLTGALTAYEASLAIRQ
                     RLAAADRSNTEWQRDLAVSHLRVALVQARQGKKSAARDGYERARAIIVKLIALSPSDA
                     TLPNDLAWLDAQLKTLK"
BASE COUNT         3024 a         5531 c         5733 g         3113 t
ORIGIN      
        1 cttcgcctcc aatgtggcgc gcatgcgctc ggtcgcggag gccgcgctgg ccgccggccg
       61 gcaggtgatc gtggccggac gcgccatgga gcgcgttacg gaagtggccg gcgaactcgg
      121 catgctggaa gggctgaagc cgttccgcgg tcccgacgtg tttccccggc tggcgcgcga
      181 caacgtcgtg gttctcgtca caggcagcca gggcgaaccg cgcgcggcgc tcgcgcgcat
      241 cgccgccaac gaatatcccg acatccgcct tgcctccggc gacaaggtga ttttctcttc
      301 ccgcgcgatt cccggcaacg aaaagggcat caacgccatc atcaatgcgc tggcgaagca
      361 gggcatcgat atcgtgacgg atcgcgatgc gctggtgcat gtctcgggcc atccccgccg
      421 cgacgaggtg cggcagatgt acgagtggat tcggccgaaa gtggcgattc cggcccacgg
      481 cgaggcactc cacctgacgg aacaggccaa gctcgccagg agcctcggcg tcgaacatgt
      541 cattcgcgga ttcaatggcg acatgatccg actggccccg ggcgatcccg tggtcaccgg
      601 gcaggcgccg agcggtcgtt tggtgaagga cggagacctt gtcaccgccg tagatggcgc
      661 cgtgcaggaa cggcgcaagc tctctttcgc cggcatcgtc accgtggctt tggccctgga
      721 cgagcgcggc gagattgtcg ccgatcccga aatcgagacg acaggcatac cggacaaggc
      781 cgccaacggc gcggacatcg ccgaactcgc cctggacgcg gtggaatccg ccttcggttc
      841 cctgagcaag gcgaaacggc gcgatccggc gaccgtgcag gaagcggtgc gtcgcgcggt
      901 gcgcgcggcc atcgcggacg tgtgggacaa aaagcctatt tgccaggtct tcgtgcttga
      961 ggtttgaacg gcgcgaagcc aacggagctg gaacatgatt ggacgcctga accacgttgc
     1021 catcgcggtg aagaaccttg aggctgccgc caccacctat cgcagcgcgc tgggcgcgcg
     1081 cgtgtcggcg ccgcaggccc tgccggagca cggcgtcacg gtcgtgttcg tggaattgcc
     1141 gaacaccaag atcgaactgc ttgagccgct gggcgcggat tcgcccatcg ccaggttcct
     1201 ggaaaagaac gcggacggcg gcatgcatca tgtctgctac gaggttgacg acattctcgc
     1261 ggcgcgggac cggttgaagg caggcggcgc ccgcgttctt ggtgacggca atcccaggat
     1321 cggcgcccac ggcaagccgg tgctgttcct gcacccgaag gacttcaacg gcacgctggt
     1381 ggaactggag caggtctgat ccatgaaagt cacgacctgg ttcgctgtct atttcgtcat
     1441 ctggtggctg actctgttca ccgttctgcc attcgccgca cggcgcaagt tggaggtgga
     1501 gcctgtggcc ggtgccgatc ccggcgcgcc gccgaagccg aatttcgtgg cgatcatgtt
     1561 ggcaacgacg ctcgtcgccg ccgtcgtgtt tgccatcgtc tggtggttta tcgagaacgg
     1621 ctacgtatga ccgaccagat cgcggcgatg agcccaccca agacgccgag ggttgggccg
     1681 atcatgaata caaggcccat cgaggccccg ccgtcgcggt cgatgaaacc tgtcgcggtc
     1741 atccaggcca ctccggccgc cacggtcgcg acccaggtta tgacaagacc ggcgaagaac
     1801 cagaggacgg cgaagccaag ggtgcgcatg ttgtcaggct cgctggcgat catggccacc
     1861 tgaaggcagg gatcacgcct ttttcaggaa ttccgtccgc agcacgagac ccttcacctt
     1921 ctcgacgcga cactcgacct catccggatc gccggtcaac cggatgccct tgaccagtgt
     1981 gccgcgcttc agcgttaccg acgtgccctt gaccttgagg tccttgatca gggtgacgtt
     2041 gtcgccgtcc ttcaggacgg cgccgttcga atcgcgcgtt tccaaggaat cctcagtgcg
     2101 gcttgctggc agtcgggtgc tggatcttgt cgacccaggc gatgccgatc gccgacagga
     2161 tgaagatcac gtggatgatg gtctgccaca tgatgccggc ctcggtatag ttcgaattcg
     2221 gcttgccgag gttgccggcc tcgatgaagg tccgcaacag gtggatcgag gaaatgccga
     2281 tgatcgccag cgccagcttg atcttcagca cgctggcgtt gacgtgatcc agccactccg
     2341 gttggtcagg gtgccccttc aggtggaggc gcgagacgaa tgtctcgtag ccgccaacga
     2401 tcaccatcac caggaggttg gagatcatga cgacatcgat cagccccagc acgatcagca
     2461 tgatctgttg ctcggtcagc ttgtacgcat cgcccacgag atggaccagt tccttgagga
     2521 acaggaacac gtagacgcac tgcgccacga tcagcccgag atagagcggc agttgtagcc
     2581 agcgcgagcc gaagacgagg cgaggcaggg gccgaaggcc ggcgaaatcg gatacgtcgg
     2641 gcttgtgcat ggtgactggc gcttgctttg gaagggcagg cgctgacata gcaccgcctc
     2701 ccacctcgtc aacgggcctg catcaggttc gcgtcgatca gccgacggtg aaaggcaagg
     2761 acctcctgct gatgcacgca gtcgctatag cgccccttgc acacatcgag cagccgtcgg
     2821 cgctcgcgat aggagtccgg cagcggcagg cgagccgatt ccagcagcag cgtcgagaga
     2881 taggccacat ccaccagctc atgccgcggc agggggggag gcgcgtagtt gacaccattg
     2941 acggcgtaga acgtgtgaaa cccgatggaa tccggcggga tgacgacgtc ctccgaatcc
     3001 gaattctccg cgaagttgag cagcatccgc gtcgacatgg gttgatggtc gccgtacatc
     3061 acgacgagga accgctcgct cgggaaccgc cgggccagtt ctttcttcag cttgtcgtag
     3121 tcgatctttg cgatcgagac gcgccggagg aattcgtgca tttccggatg cgtgccttcc
     3181 agcccgcccg gcacctccat ttcaggcgca taggtgaagt tgaacggcca gtgggccgac
     3241 atcgtctcga tgaaggtgaa aagcggctgg cgcgactggg cgaagtggcg ctccatctcg
     3301 tcgagcgcgc tgcgatagta gaaggcgtcc cgctcgttga acgtcggtgc gttctggccc
     3361 cgcttgtcac ggatatcggt caggcccacg ccctcgaaga accgcccggt ctgcgcgaag
     3421 ttcttcagca ttgggtagaa catcacattg cgatagccgc accgttccag gacatgcggc
     3481 agcgcttcgc ggatcttgcc ggccatcacg gtctggagga actgccgcat gccgccgaac
     3541 gagcgcgtcg agagcccggt gagaatcgag aactcggtca ggttcgaggc gccgccatag
     3601 gtttcgaccc tgagcttgtg caactggccg tcatgggagc ggaagaacgg gtccatgctc
     3661 cggtcgtagt tgaggttcgc gaagaccgaa ggcggaaaca cgctttcctg gtggatcagg
     3721 atgatgtgcg gcggcttgaa ggcggtgttg catcgctcca gcggggcaag cggcggccca
     3781 gccgcagcat gcgccgcatc gatcagctgg cctttccaca gcgtctccag cgtttcctgc
     3841 caggagatca ggaacgagga aatgaactgt ccctgatact cgaattgctg atgcctgcgc
     3901 tcgcccatcg cgatgactcc cgccaccgac acggcaaatg cggccactgc aacggcggcc
     3961 gagaggctgc gccgggccct ggtcggatcg gcgcgaaaga tcacccagcc cagcccagcg
     4021 accgtgagca gcacgaaggc cagcagcagg aactgatccg agaactcggt gagcaggtac
     4081 ttcaccgtcg gccagtcgct taggtaaaac accagatcat aggcatgcag caccatgttc
     4141 atggtctgaa gcttggcgat ggcggccagc ttgatggtga caatgatcgc cacggtcagg
     4201 aaggtcgcga acagcacgcg ccgggtcgcg aacgcgagca gtgcggcaaa gctgatggtg
     4261 ccgccaaagg cgatgagctg tgtcggccaa cggccctcgg tgcggacgta gttgaaggcc
     4321 aggacgacag cgatgatcgt cacgatggac caggcccagc gcgccggaat gccatggcgt
     4381 gccggccagg cgcgccacgt gtcgcgtgag gcctgcgaac ggatcggaga tgattcgaac
     4441 gatatgtcgc tcacggcggc tgttcgtgtc ctggcggcga ccagtgtcgc cgaaccgtca
     4501 tttagacact tttatgaata gccggcaatg aataaccgat gaagattgca ccgtatccgt
     4561 ggcctgagcg gatgcgactt caggccggaa cggctttccg ccccgctcga tggcgcgcga
     4621 tgatcgtctt catgatctgg gccgtgccgt cgccgatctg gaagccaagc acgtcgcgca
     4681 gtcgctgttc catcacgccg cgatcatagc cgccatggcc gaaggtgagc agggcggcat
     4741 ggatggcgtc gtaggcgagc ttcggcgccc accacttgca catcgccgcc tcggcggaat
     4801 gcggcaggcc cctgtccttc aaccagagtg cctggaagca gagcaggcgg gccgccgaga
     4861 cctgagtgtc gaagtcggcc agggggtgcg agacgccctg gaaggccgaa atcggcttgc
     4921 cgaacgcttc gcgctggctg gcatatgccc aggcttcgtc gagggccgcc cgagccaccg
     4981 ccagcacctg caggccgatc agggcgcgcg agaagtcgaa gccctgcatc acctggacga
     5041 agccttcgcc ttcgttgccg agcaggtggt cggccggcac gcgcacacca tcgaagaaca
     5101 gcgagccccg tccgatggcg cgttgtccgt gacagtcgaa ccgggaacgc gtgaggccgg
     5161 cgaggtcggt ggggacaagc agggccgaaa tgccgcgcgc gccgtcctca atacggccgg
     5221 accgggcgaa gacgacgacg acatcggcct ggtcggctgc ggaaatcgag gttttctccc
     5281 cgttgagcac gaagtgtgcg ccgtcgcgtt ccatcttcag acgcatcgcg ccggcatcgg
     5341 agccgccctt cggttccgtg acggcgatcg cgcacagtgc ctcgcccgca aggatccgat
     5401 ccagccatgg ccgggtcacc tcggcgcggc cgtagcgcga caggatctgg ccgttcagcg
     5461 aggccagcag gttgatgtag gacagcgaga gatcggcgcg ggcgatctcc tcgtggatga
     5521 tgccggcagc cgtgcaggtc aggcccagtc cgcccgacgc ttcgggcagt tccggtgcga
     5581 tgaaccccat cgcgcccatc tcgcgcatga gagcgcggtc cagcacccgc gtgcggtcgc
     5641 gttcctggaa gcctggcgcg acccgggctt cggcgaactt gcgcgcctgt tccgcgagcg
     5701 cctggagatc ggcgtcgatg taggggttca tgacggatta cttctggtgc ttgcggaaat
     5761 ccggcttgcg cttctcgttg aaggcgcgaa cgccctcctt ggactcctcg gtctcatagt
     5821 agagcttgag cgcatacatg cccatgccgg cgatgccggc ctgatgcgcc gtgtccatgt
     5881 tgaaggaacg cttggcgatg gcgatggcgg tcggcgagcg ctcggcgatc tcctcgcacc
     5941 acttctgcac ttcggcgtcg agctggtccg gcgcgaccac ggcgttcacc agccccatgg
     6001 caagggcctc atcggcggtg tagcggcggc agaggtacca gatctcccgg gcccgcttct
     6061 cgccgacgac gcgggcgagg aaggccgtgc cgtagcccgg atcaaccgac cccaccttcg
     6121 ggccgacctg gccgaactgc gccttctgcg acgcgatggt gaagtcgcag atggtgcaca
     6181 gcacattgcc gccgccgatg gcgtagccct gcacacgggc gatcaccggc ttcggcacat
     6241 cgcggatcgc ggcatgaagc tcttccatgg gcaggccgat cgtgccgcgc ccgtcgtagg
     6301 cgccggcgtg ggccgactgg tcgcctccgg tgcagaacgc ccggtccccg gcgccagcca
     6361 gcacgatggc cgcgatggac ttgtcatagc ccgcccggtt tagggcatgg atgagttcgt
     6421 cgcaggtctt gccccggaag gcgttcatct tgtccgggcg gttgatgatg atccaggcgg
     6481 ccgggccgcg aacctcatag agaatgtctt cgtacgtcat cagccttgct cctgaatcct
     6541 ggatcatcca accatggtga ggccgcccga gacgctcagg acctgcccgg tgatgtaggc
     6601 cgcgtcgtcg gaggcgaaga acaggatggc gccgggcagg tcatccgggc ggccgatgcg
     6661 ccccagcgga atggagcgct cgaaggcctc gaccagcttc tccgggtttc ccgcgccctg
     6721 cttgtagccc tcgaacaggg cggtgtcggt cggcccgggg cagaccacgt tgcaggtgat
     6781 gccgtgtcgt gcatgttcac gagcgatggt cttggagaac gacacgatcc cgcccttgca
     6841 ggcggcataa accgcctcgc ccgaagagcc gacgcgcgcc gcatccgatg caatgttgac
     6901 gatgcgcccg gccttgcgtg ccaccatgcc gggcagcacc gcgtggtgca ggttcagcgc
     6961 gccagtcagg ttaatggcga tcagcttcgg ccagtcctgc ggcgcggttc tagtgaacgg
     7021 cttgaacacg tcccagcccg cgttgttgac gaggacctgc acggggccga gcgccgcctc
     7081 ggccgcggcg acggcggacg cgacctgctc gtagtcggtg atgtcgcagg cgagtggcgt
     7141 ggccgtgccg ccggcggcgg tgatgtcggc tgcgaccttc tcggcggcgg cgaggttgag
     7201 atcgagaatg gcgaccttcg cgccctcagc ggcgaaacgc cggcacgtcg ccccgccaat
     7261 gccgccgcca ccgcccgtga cgatgacgct cgcccccgaa agcccttgca tgtgtcgccc
     7321 ctgatttgca aattgatata tcaatgctat gctataaaaa attcgacacg tcaatgagag
     7381 tttcagggtg ttagcgatgc aaaatgagcg ggtgcttgct cccggagcca ggaaggcgga
     7441 tgacgctgat ctgcgccatg atctggcgaa ccgccttttt tttcgccttt accaggcttc
     7501 gaacctgctt cataaggttg ggaccaaggc ggtttccggt ttcggcgcga cgacgcaaca
     7561 gtgggccgtt cttggcgctc tggctcgcca gggcaacgag cggcaaggat tatccatcaa
     7621 gcaattgatg gaatatttgc tcacgagccg tcaaaatctc acgctggttc tggatcggct
     7681 cgaagggcag ggactggtgg aacgcgtgcg cggaacgacg gatgcacggg tccgccatgt
     7741 ccggctgacg gcacagggcc gcaagacctg ggaagagatg ctggtcgaca tccgcgcgtt
     7801 ctacggtcag gcgacggaag gtttctcgac cgaggaggtg ttcctgctgt tccgactgct
     7861 cgatcggctg aaagaccggc tgtcagcctt gtgaggccgc ctgttgccgg tcgcggatac
     7921 ggcgcgccac ggacttgagc agccagggca tcagatagat cggcacctgc gcgagcgcga
     7981 agaacaggta ggtcgaatcc ggcacgccgg cgccgagcgc ggcgatcagc agccccatgt
     8041 tccgctggcc agttccatag cccaggatga agcgctcggc cggattggca aagcgcagca
     8101 gcgccatcgc cagcacgagc cccagcgcag taacggccag tgccgcgccc aggaatccgg
     8161 cgacccgggc cggttcgcgg aacgccgctt cggtgacgcc gtccatggcg gcgatggcga
     8221 acaggaagta catcaccacg ccgaacccgt cgaactctgc cttgagttcg gcgacgcgtg
     8281 gggtgccgag ccagcgccgc agtgccatgg cggtgaggaa tccgccaccg acgaacaccg
     8341 ccatgcgcat catcagcacg ctttgatcca gaggcaccgc ctgcccggcg atccagtcga
     8401 ccagcagggg agacacaagc ggcgacaaca ccgtgatgcc gaggacgcag gcgatgatca
     8461 gcgagggctc gaagccatag agaatggcga ccgccggcga cgacatgatc ggcggtgccg
     8521 ctccaaggat ggcgaggccg agcagaaggc cgggatcgat cgactcacgg ccgaccgcca
     8581 ggatggcgag catggcgatc aacgcaggca tgcagaccag ccacaggcag gagagagcaa
     8641 gcttggccgg ccggcgcacg aggccggcga tcaccgtgat gtccgccctg aggaacacga
     8701 tgaccatgaa gcagaagatc gtcaccggca gcagcggccg cgcacgctcg gcgaactgcg
     8761 gcagcgccag ccccatgaac agggcgagcg cgaatccttc cgtgccgtgc cgcccgatgt
     8821 aggcgagtgc gctggcgagg cttttcaagg ggctgggctc cggcaagggt ctgtccgggc
     8881 aaggcgcccg cgagtcgatt tgatcatagg cccgacgccg gatggaagcg actgcaatag
     8941 cgcgtccggg ccccggtcct gccacgcttg acttggcctt tcgcgtggct cctgatagtc
     9001 atcagacaac cggccgtgcc ggatcataac cagacgggag gaaccatgaa acgcctgttg
     9061 ctagccgccg cttctgtcgc agccctcgcc gctcccgccg ccgcgcagga catccgcatg
     9121 atgacgggcc cccagggcgg catctgggtc ccgctcggcg gccagttgaa ggacctgtgg
     9181 gagaaagcgg ttcccggcct caaggtcgcg acgcttcccg gcgccggcgt cgccaatgcc
     9241 cgcgccatcg aggaagccaa ggcggaggtc gggttcggca acaccatcac cacggccgac
     9301 gccgtcgagg gcaagccgcc cttcgacaag aagcatggaa agctgtgcaa cgtcgcctcg
     9361 ctctacccgc agtatttcca ggcgatcgtg cttgccgaca gcggcatcaa ttcggtgaag
     9421 gatatcaagg ggaagaccat caccacgcag cagcgcggca atacgggcga attgataacc
     9481 cagcactacc tcaaggccca cggcctcagc tattccgacg tgaaggtcaa tttcggctct
     9541 tacaccgaga gcgtcgagca gatgaaggac aagcaggccc atctgttcac gctcggcgcc
     9601 ggcatcccgc tgggcgctgt catggacctc gctgccggcc gcgacatcaa gcttctggac
     9661 cagaccgaag cccatgccga aatgaagaag atgaaccccg gctatgtgct gcttccggtg
     9721 ccgaagggca cttatcccaa gcaggagcag gaggtgaagg tcattgccta cgccacgcat
     9781 gtggtggcgt cctgcgcgct gccggccgat acggtctaca ccatgaccaa ggcgattgcc
     9841 gagaacaccc agctgctcgg caacctcaac aaggacatgg gcaagctgac gccgaaggac
     9901 atggccgaga atgtcggcgt gccgttccac cccggcgcgg cccggttcta caaggagaag
     9961 ggcatcacgg tgaactgatc ggcggcacgc ggtcagggcg gcggcgtgaa cgccgccgca
    10021 ggtcgggggg aacgcaatga ccaatgcggc gcggcgggcg tcggtctgga agaccgttat
    10081 tggcgtgatc gcctgcgtca tggccctgta ccacctctgg gtcgcctggt tcggtgcacc
    10141 ggagcagcac ctgttccgcg gccagcatct gctgttcgcg ctggtgctga ccttcctgtg
    10201 gtaccggcgc acgacgccga ccgaggacga actggcgatg cgcctggagc agggcggctt
    10261 cattccgccg cactggagcg acctggcgct ggttgtgctt tcggcggtgc cgttgctcca
    10321 cctgttctgg aactacggct atttcgtcaa ccgcatctat tacgtcgacg atctgagcct
    10381 gttcgatcag gcctgcgcca tcgtgctgat gctcctgatc cttgagggag cccggcggct
    10441 tatgggctgg gcgctgccgc tgacggcgct ggtcttcctt gtctacgccc tggccatcgc
    10501 ccgcgtcgaa ccgatgcgca tgctcgatca gctctacatg acgaccgagg gcatcttcgg
    10561 catgtcgctg ggcgtttcgg cgtcctacgt catgattttc gtgctgttcg gcgccttcat
    10621 ggaacgcacg ggcaccggcc agttgttcat ggatttcgcc atgtccatca ctggccacac
    10681 ggccggtggt ccgggcaagg tctcggtcgt gtcgtcttcg ctcttcggca ccatttccgg
    10741 ttccgccgtg gccaatgtca tggtggacgg tccgatctcc atcccgttga tgaagcgcac
    10801 cggcttcaag ccgcacttcg ccgcggcggt ggaagccgtg gcctcgaccg gcggacagat
    10861 catgccgccg atcatgggcg ccgcagcttt cgtcatggcg gagttcatcg gcgtcagcta
    10921 cgggcaagtg gtgatctggg cggcgatccc ggcgatcctt ttctacgtcg cctgcttcgc
    10981 gtcggtgcat ttcgaggcca agcgcaccgg attgcacggc gtgccgcgtt ccgagttgcc
    11041 gaaactgggc gcgaccctgg ccgaacgcgg ccatctgttc ctgcccgtgc tgaccatcct
    11101 ggcggtgatg tacgctggct acagcgcgcc gctggcggcg ctggccggaa cgctggcctg
    11161 cttccccgtg gcggcgctgc gggcgtcgac gcgctccaac gtgagcgtga agaacgtcgt
    11221 cgaagcgctg atcgacggcg cgaagaatac gctggcggtg gcgctggcct gcgcctgcgc
    11281 cggcatcatc atcggcatcg tcacgctcac cggcctcggc atcaccttca cgcagttcgt
    11341 cgtcggcctg tcgaaggaca cgctgctgct ggcgctgttg ctgaccatga tcgccgggat
    11401 cgtgctcggc atgggcatgc ccacgacgcc ggcctatatc atcatggccg cgctgctgat
    11461 cccggccatc atcaagcttg gggttcccaa gccggcggcg cacatgttcg cgttctactt
    11521 cgcaatcctg tcggccatca cgccgcctgt ggcgctggcc gtttatgctg cggccggact
    11581 ggcgaagtcg gacatctgga aaaccggctg gtccgccgtg aaggtcggcg cggccgggtt
    11641 catcgtgccc ttcatgttcg tttacgagcc ggctatcctg atgatcggca cctggccgga
    11701 catcatccac gccacggtct cggccatcct cggctgcctg cttctggcgg ccggcctcgc
    11761 cggctactgg ctcgcgccgg ccaatgcggc gcagcgcctt ctgcttatcg ccggcgcctt
    11821 tggcctggtc acgccgggcg tgatcagcga tctggccggg gcagggatcg cggccgttgt
    11881 cacgctctgg caactcgcgg gccgcaagaa ggcacaggaa gcggggcctt gagcttcagc
    11941 gacccttgaa cccggaaggg cggtgggcga tcagtccttt tcgcgcatca ggcgcttctt
    12001 cgcctgcgcc agttccgccc gttccttgtc cgagatctcg ggatacttga tgtcgagtcc
    12061 gcgcagctcg tgcaggatcg cggtaacgac cgcgacgcgc atgaaccact tgtcgtcggc
    12121 cgggatgaca taccacggcg cgcgcttgct cgcggtctgg cggatcgcgt tggtataggc
    12181 cacgcggtat tcgtcccaca gcgcgcgctc gttcaggtcg ttcaactcga acttccagtt
    12241 cttttccggc tcgtcgatgc gttcgatcag tcggcgcttt tgttcgtctt tcgagacgtt
    12301 caggaagaac ttgatgactt tcgtgccgtt gcgcccgagg tagcgctcga actgccggat
    12361 atcttccagc cgatggtcgt agatgtccgg gtcctccttg aggtcctgcg gcagatgctg
    12421 cttggcgagg atttcgggat gaacgcgcac caccagcact tcttcgtagt aggagcggtt
    12481 gaagatgccg atccgccccc gttccggcag ctgcctgacg cagcgccaga gatagtcatg
    12541 gtcgagttcc tcgtccgaag gccgcttgaa cgccgcgaca tggcagccct gaggattgat
    12601 gccggacatc acgtgtttga tggttgaatc ctttccggcg gcgtccggcg cctggaagat
    12661 gagcagcact gaccattcgt cctgcgcgaa cagccgctcc tggatctccg aaagttcctt
    12721 gatgccctca tccaggacgc ttctggcttc cgccttttcg tccatggcgt cgccgagcgg
    12781 gttctcggtc tcgtagtcgt cgaggtcgaa tcccctgcca tcgcggacgc ggtacttctt
    12841 cacgagtctg acaaggtcct tggtcggcat cgcgtttctc cggcgtgtcc ggcaaacatg
    12901 gcagtccggg cgagggaaga caatgcgcgg ttcgcttgca tccggacggc gcatcggcat
    12961 aaccggcggc atgcttatca tctacgattg cgacggcacc ctcatcgaca gcgaaggcat
    13021 tgcctgctcg gtctgcgccg aggcgctgag cggtctcggc gtcccctaca ccacggagat
    13081 gttcgccgcc cgttatgccg gccgcccggc gcgggaaacc tgggaccatg tcatcgcgac
    13141 ctatggcgtg accctgccgg acgggttcaa cgcgcgcatc aatgccgaga tccatgcccg
    13201 cctcgatgcg ggggtcgagg ccgtggaggg cgcgcgcgcc gccgtcgaag cgctggcggg
    13261 gccgcgctgc gtcgcctcat cgaccggcct gacgcagttg cgcaagaatc tggcgacggc
    13321 cggcctgatc gacctgttcg gtccgcatgt gttttcggtc agccaggtga agcgtggcaa
    13381 gcccgccccg gacgtgtttc tctatgcggt atcgcaaatg gggttcgatc ccgccgatgc
    13441 cgtggtgatc gaggatacgg ttccaggggt cacggcggcg cgtcgggccg ggattcgcgc
    13501 catcggcttc accggtgcca accacggcgg agacgggctg ggcgatcggc tgaggcaggc
    13561 cggcgcgctg gacgttgtgg ccgcgatggc cgatctgccc ggcgttgtcg catccctgcg
    13621 ccgctgatcc ctcaacgatg gtagcctttg cgagagcgca cgaaaacggg tatagggcgc
    13681 cgccatgacc agcggttttt atcccggctc cttcgatccg ctcacaaacg ggcacatcga
    13741 tgtgctcaag agctgttttc cactggtcga tcggctggtg atcgccgtcg gcatccatcc
    13801 cggcaaggcg ccgctgtttt ccgccgagga acggcttgag atgctcaagg tctgctgcgg
    13861 gccgctggcg cagcaggccg gcgtcaggct ggaaacgacg accttcggcg atctgaccgt
    13921 atcggctgcc cgccgttcgg ggtgcaacat catcattcgc ggcctgcgcg acggcaccga
    13981 tctcgactac gagatgcagc ttgcaggcat gaaccagacc atggcgccgg agattcacac
    14041 ggttttcgtg ccggcttcgc cgctggcccg gccgattacc gcgacgctgg tgcgccagat
    14101 ctcggcgatg ggcggtgacg tgtccggatt cgtgccgccc tatgtgctgg agcaacttcg
    14161 gttaaaatcg gcgccacgct gatttgattc gaaaccgaag gacttgccat gcttcgtcgc
    14221 acgcttttcg ccgccgcgct tgcgctcctg cccgtagcgg cgctggccca gcagtcgcgg
    14281 caggatgccg agaacaccat tcacctggaa ctcaaggacg gccgcgtcgt catccgcctg
    14341 cggagcgatc tggcgcccaa gcatgccgag cagatcaaga cgctggcgcg tcgcggattt
    14401 tatgacggcc tgacatttca ccgcgtgatc gccgacttca tggcccagac cggcgatccg
    14461 aatggcaacg gaaccggaag ttccaatctg ccgaacctgc cggccgagtt ctcccgcgag
    14521 ccgttcaagc gcggaacggt cggcatggcc cgggcctccg atccgaattc cgccaactcg
    14581 cagttcttca tcatgttcaa ggaaggcgcc ttcctgaacg gccagtacac cgtcgtcggt
    14641 gaggtcatcc agggcatgga cgccgtggac aagatcaaga agggcgacgt caatcgcaat
    14701 ggcgcggttt cccagccgga caagatcatc cggatgagcg tcgccgctga ctcccgctga
    14761 tttatcaagg aaaacaagag catgacaacc gcagccgaag acacgctgat cttcgagacc
    14821 accaagggca aggtcgtgat cgagatgcgc ccggacctgg cgccgggcca tgtcgcgcac
    14881 atcaagaagc tggtgcggga aggcttctat gacggcctga ccttccaccg cgtcatcgac
    14941 ggcttcatgg cgcagggcgg gtgcccgaaa ggcacgggaa ccggcggctc caagtatccg
    15001 gatttaaagg cggaattcaa caatgccccg catgtgcgcg gggtctgttc catggcccgc
    15061 gcgcagaatc cgaactcggc caattcgcag ttcttcatct gcttcgccga cgcccgcttt
    15121 ctcgaccggc agtatacggt ctggggcaag gtgatcgagg gcatggacaa cgtcgaccag
    15181 ttcgcgcgcg gcgagccggt gcgcaacccg gacaagatca tcaaggcgac gattgccgcc
    15241 gaggcggcct gagtcgttac tttaaaaacc tgcgccatgc gtctgtcgga cttcgatttt
    15301 caccttccgc cagaacgcat cgcgctgcat cccgcacgcc cgcgtgacgc ggcgcggatg
    15361 ctcgtggtcc ggcccgatca tgtccctgaa tttcaagaca gaatcgtcag cgacctcacg
    15421 ggtttcctgc gaccgggcga tgcgctggtg ttcaacgaca ccagggtgat cccggcgcgg
    15481 ctgtttggcc gccgtgatcg cgacggcgcc agcgttagcg tcgaggcgct gctgcatcgc
    15541 cggctcgatg cgagccgctg gctcgccttt gcccgccccg gcaagcggct ccgggtcggg
    15601 gaccgggtga gcttcggcga cgcggatgga aatgcttgtc ttctcggggc cttgcacgga
    15661 accgttgaag aaaagcatga gggcggagaa gtcgccatcg ccttcgatct gtccggtccg
    15721 gcgctggatg acgccatcgc cgcggcgggc gaaatgcccc tgccacccta catcgcctcg
    15781 gcgcgccccg ccgggattca ggatcgcacg gactaccaga caatctatgc acgagaagca
    15841 ggagcggtgg ccgcgccgac cgcaggcttg cattttacgc aggatttatt cgcccggctc
    15901 gacgccatgg gcgtgtcccg gcatttcgtg actttgcacg tcggggccgg cactttcctg
    15961 ccggtgaagg ccgaaacgct cgtagaacac aaaatgcacg cggaattcgg ctcggtttcg
    16021 gccgcgtgtt cgacgatgct cggtgaggtg cgttcgaaag gcgggcggat cgtcgccgtc
    16081 ggcaccacga cgctgcggtt gctggagagt gcggcggcgg aaaccggcac gatccgaccc
    16141 ttcgccggcg agaccgacat cttcatcaag cccggcttcg ccttccgcgc ggtcgacctg
    16201 ctgctgacga atttccacct gccgaagtcg acgctgttcg tcctcgtctc ggccttcgcc
    16261 gggcaggagc gcatgcgccg cgcctatgcc cacgccatcg ccggcggata ccgcttctat
    16321 tcctatggcg atgcctgcct gctggagcgg gccgggggat agcgccggcg cttcaaccgc
    16381 cagggcgcga tggccctcgg tgtcatcccg gaaggccgca ggcctgtccg ggatccagac
    16441 tttccttttg ccacgcgcac ggcttcacgt ctgggttccg ggttcgcttc ttcgaagcgc
    16501 cccggaatga cagggtgggc tatttcagcg ttttcaattg cgcgtcgagc caggccaggt
    16561 cattcggcaa ggtcgcatcg gatggcgaga gcgcgatgag tttcacgatg atggcgcgcg
    16621 cgcgctcata gccgtcgcgg gcggcggact tcttgccctg ccgggcttgg acaagtgcaa
    16681 cacgcaggtg gctcaccgca agatcgcgct gccattccgt gttgcttcgg tcggcggcgg
    16741 cgaggcgttg gcgaatggcg aggctggcct cgtacgccgt cagcgcgccg gtcagatcgc
    16801 cctgcgcgac gcggacatcg ccgatctttt cctgggagat cgagagatcg cgctgccact
    16861 gcgtgttgct cggatcggcg gcggcgaggc gttggcggat ggcgaggctg gcctcatacg
    16921 ccatcagcgc gccggcgaga tcgccctgcg cgcggcggac atcgccgatc ctgtcgtggg
    16981 agatcgagag atcgcgctgc cactgcgtgt tgcgcgtgtc ggcggcggcg aggcgttcga
    17041 agatggcgtg actggcctcg tacgccgtca gcgcgccggc gagatcgccc tgcgcgacgc
    17101 ggacatagcc gatctggttt tgggagaccg agagatcgcg ctgccacacg gtgttgctcg
    17161 gatcggcggc ggcgaggcgt tggcgaatgg cgaggctggc ctcgtacgcc atcagcgcgc
    17221 cggcgagatt gccctgcgcc gtgcggacat cgccgatctt gtcgtgggag atcgagagat
    17281 cgccctgccg ctgcgtgttg ctcggattgg cggcggcgag ccgttcgaaa ttggcgtgac
    17341 tggccgcgta cgccgtcagc gcgccggtaa gatcgccctg cgcgcggcgg acatcgccga
    17401 t
//
LOCUS       DAOMXN010000030        16968 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_1924_length_16968_cov_11.920594, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000030 DAOMXN010000000
VERSION     DAOMXN010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 16968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16968
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1924_length_16968_cov_11.920594"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="PK812_04400"
     CDS             <1..515
                     /locus_tag="PK812_04400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRJ68829.1"
                     /translation="LPLAVKRVCVLRTVAAPPQFEQVFGVANADCAGRQEIGGAFRIT
                     TGIGDWPGDVAGWNEAALRPSLAIVAELIARGSTILPVLSGLGLERVWGGLIDLTPDA
                     LPVLDAPHGCPGLVVGAGFSGHGFGIAPVSGEILAGLATGQSVPWDLAPFRIDRFSPG
                     RAFAPLSLHG"
     gene            522..1232
                     /locus_tag="PK812_04405"
     CDS             522..1232
                     /locus_tag="PK812_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007812487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRJ68830.1"
                     /translation="MLDPKGRVILVSGASRGIGRAVAETLHAQGYALSLGVRDPSKAA
                     AALSALAGERLHLARFIAEDWTSHARWIAAAAERFGRIDGLVNNAGTNSQMTLRTPDE
                     AELDRVWAVNCKAPLNLIHHALPHLEASGAGRIVNIASLSGKRVRNDRVAYNMSKFAL
                     MALTHSARRIGWDKGVRATAICPSFVSTDMSLVASFPFDQMTTPGSVAELVAAVLALP
                     NNAAIAEVLVNCRLEDTL"
     gene            1272..2822
                     /locus_tag="PK812_04410"
     CDS             1272..2822
                     /locus_tag="PK812_04410"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRJ68831.1"
                     /translation="MKTTWLASATIAALLCATPALAQKTTLVVGMQTQDAGVLDPHLN
                     SGTPGKALMNWMFNGLVRIKPGQSSPDHIEPDIAESWTSSPDGKEWTFKLRQGVQCHH
                     DLGEFTSEDAVFSLKRAGTKDTSAFSSDYAAFDKVEAIDKYTVKITLKNAIPSLLGQV
                     MSYHGGNMVCKKAADGPDKDGFAKKPAGTGPFVFAEYVPQQYVKLVANKKYWRGEPKI
                     KEIIYRFIPSDAARDLALQAGEIDLMSGRQEQAWVDRIARTPGLKVLTFGPAEMSVIH
                     FNMTQPPLDNIKVRQAIAHAIDRAGIHQFRGMSISRPAVSVVPSGYFGTHETAPLLPH
                     DLNKARQLLTEAGFPNGITIKAIHTTLPGMMSFMEATQAQLKRAGITLDITPVEHPTF
                     HQQIRQDLSQVVHFQAARFPIADVYLTHFFHSSSIVKTPTAVTNFSHCKVADAEITAA
                     RTEQNKEKQVALWKTAQEKIIAEVCAVPVNEMLMNWAHRDNLDLGYELKGSLNLGPPI
                     TENTRFTR"
     gene            2883..3821
                     /locus_tag="PK812_04415"
     CDS             2883..3821
                     /locus_tag="PK812_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ68832.1"
                     /translation="MGAFLVKRLMFSAVTLFAVLTLVFIVIRIVPGDPALVILGDQAD
                     AAAISALQKRLGIDRPLFEQYLSFLSGAVRGDWGVSLVTGRPVLMEVLAVLPWTLELT
                     AVALLLGAMIGVPLGAWAAINRNRMPDYLTRLLSLVGLSFPPFVSAILLLIGFALALR
                     WFPVISSNRATWWDWFQSIALPALNLALIMAAYITRVTRSAMLEVLQEDYVRTARAKG
                     VPWAVIVWRHALRNALIPVVTVVGLYLSILIGNSVLTEIVFNRPGLGKLIIGALNQRD
                     YMMLQGMMVIYTLIVVVVNMLTDIVYAVVDPRVKLS"
     gene            3818..4708
                     /locus_tag="PK812_04420"
     CDS             3818..4708
                     /locus_tag="PK812_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003523912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ68833.1"
                     /translation="MSGSIHDVPDAAPERLRWLKATVSAFNANKTSWFGLVVFAIVVV
                     LAVLAPWIAPHDPLEQNVFSKLRPPFETYYLGTDYFGRDILSRLLHGARISLVIGILA
                     TIVALVVGSMIGIVAGWYGGRLDIIVMQTMDMLLAFPALILGLILVAMLGPSIGNIVI
                     AIAITSIPSFARIARAPTIAVKEREFIDAGRSLGFSDLRLMARHVLPNIFPEILVMGS
                     LWLASAIRTEASLAFIGLGVKPPIPTWGGMIREGFENILESYWLVLAPSIAILVVVFA
                     LNILGDGLRDAIDPKLKGER"
     gene            4705..6522
                     /locus_tag="PK812_04425"
     CDS             4705..6522
                     /locus_tag="PK812_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012321501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ68834.1"
                     /translation="MSAPLLSVEGLRTAFRSGGEWRDAVDDVSFTINAGETLAIVGES
                     GSGKSVTALSIMRLISSINGRIQGSVRLEGRDLLALPEEEMRRVRGNAISMIFQEPMT
                     SLNPVLTVGRQICEALYYHRGMDARAAEAEAIRILDRVRIPASRQRFGDYPHRLSGGM
                     RQRVMIAMALACRPKILIADEPTTALDVTIQAQILDLIKALQEEEGMAVLFITHDMAV
                     VAEIADRVAVMWKGKKLEDRPAAELFAAPHHGYTKALLAAVPRLGQMEGHERPLRFAV
                     IDPGTGVTQVPATSVPDTISGETTPLLQVSGLTTRFPIRKGVLGRLAGRVHAVENVSF
                     DLRKGETLALVGESGCGKSTTGRTILRLVEPTSGDVRFGGTLVGGLPRDDLQVMRRRM
                     QMIFQDPFASLNPRKAVGAAIAEPMVVHGVADAKEAEERTASLLRRVGLLPGMAYRYP
                     HEFSGGQRQRIAIARALALSPEMVVADEAVSALDVSVKAQVLNLMLDLQAELGLAYLF
                     ISHDMAVVERVSHRIAVMYLGEIVEIGPRAEVMRNPVHAYTRKLLSAVPVPDPARRRK
                     RGTVAVEELPSPIRPPDWRAPPTRMEAVGPDHFVQVVEG"
     gene            6700..7086
                     /locus_tag="PK812_04430"
     CDS             6700..7086
                     /locus_tag="PK812_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68835.1"
                     /translation="MADPYPAPDATPPLRVLILGASGPGWYAASQQERAERILPRLAA
                     VFAAWTALGARLIVTLDDDLLKVGQPADHDHTWYLVYDVPDLQTVSTMLHQFRLEVDG
                     ARLDRWFRLEAKVCRPFFPAEAVAQP"
     gene            complement(7077..7739)
                     /locus_tag="PK812_04435"
     CDS             complement(7077..7739)
                     /locus_tag="PK812_04435"
                     /inference="COORDINATES: protein motif:HMM:NF014063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033735 - aspartate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF108 domain-containing protein"
                     /protein_id="HRJ68836.1"
                     /translation="MTPVNLIGHGTIGAGLADWLARSDRYTLNSVIRRGGGWSAAPLT
                     IDAAGPEALRAFGTALLAQGDLWSVGAAALVDDDLRAHLLETARQHRHELRLFTGWIA
                     GPALCAAGSGARLHVEQHAPRLGPAPGLVFSGSLRAAAARYPDVLNTATAAALAGPGI
                     DATSVALHCSPVSGPHCIRARCEVPGQTIVTSVDFSEGPHPVTQALIAALETRGQYLR
                     YG"
     gene            complement(7801..9060)
                     /locus_tag="PK812_04440"
     CDS             complement(7801..9060)
                     /locus_tag="PK812_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008191549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRJ68837.1"
                     /translation="MRLRILGSMAGAAMALLAPAGGAAAQECSVKVGAIFPTSVDWGK
                     PIAETALWVADMINQAGGVDGCKVETILRDDQNDAKVGVDAAKALVDISKVQLLLGTV
                     ASGITIPILTSVSVPAGTMQMSCCSSSTRLTAMANEGATKGLWFRTFATSSVQGAVAA
                     MAAKEAGYKKVTIFFKNDDWGQDIAKIAANAFAKSGIEVTTQIAITDGQPSYRAEVAR
                     ALAAKGDAVYLALYPKEGIAVVREWLSLGGTTNMIGANALKSDDFRNTVGMQHLANFV
                     GTDTSTPRTDSAKGFVAAFTAKFGKAPAGPGLPNSFDAAAISLLAYHAAGRNATGAQI
                     AAKVGMVTDPKGEKVNGDVDGFKKALGLLKQGKTVSFQGGTGAVAFDKNGDVSAPAVL
                     WTFGASGVQEKRYITLEEVGAFISSVK"
     gene            complement(9062..10039)
                     /locus_tag="PK812_04445"
     CDS             complement(9062..10039)
                     /locus_tag="PK812_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008193854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRJ68838.1"
                     /translation="MSGVVSYLVFFATMAGILGIVVLGLNLQWGYTGLFNGGVAAFFG
                     AGAYVTMILGGRPEPGQFGGFQLPFVIALAGGIASAGLAAWFFGRLTLRLRHDYLAIA
                     TFGVAVAFESVARNAEWLLGGAKGLRGFPRPLEAAIGNNFLYGLFFLTLVMAVLLAVY
                     VGLERLVHSPYGRALRAIREDEIAAQSLGKNPARLRLQSFVIGSMIMGAAGGLYVAFY
                     AFVSPQDLAPVLTFQVWAMLIVGGAGNNLGAILGAYLIWAAWTASGFLLAAFAPQAMQ
                     LYTGPIQYILIGSVIVGMLLWRPQGILPERIARSGHRASGATTADNGGN"
     gene            complement(10036..10950)
                     /locus_tag="PK812_04450"
     CDS             complement(10036..10950)
                     /locus_tag="PK812_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008194752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRJ68839.1"
                     /translation="MIQSLLDGILTGAVISLGAIGLTMVMHMLRFANFAYAELLAIGA
                     YAALVFDRLFAALVPALGTAFKPLTLSASLTLATLLAMAITGASAVLIDWLIFKRIRA
                     KADALSMVFASFGVALIIRNVITLVFGHNAQLYSPDIAFAVVLSLDPLVLVKPDQVFV
                     LAAALALMAAFHVVLTRTTFGFALRAVAENPALAQVAGVRLSTMIAAVWLIGGGLSAA
                     AGVFYALTNHLSPALGHNFLLPVFAATIVGGIGSVYGAVLGGFIVGIASGLSLMVLPS
                     GYVPAMPFLIILGVLLVRPHGLFGEDLA"
     gene            complement(10947..11672)
                     /locus_tag="PK812_04455"
     CDS             complement(10947..11672)
                     /locus_tag="PK812_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006938952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ68840.1"
                     /translation="MSAAIRIEGMEAGYVRDLPILRQVNLSAQRASLTAIIGPNGAGK
                     STLIKAIAGLVPVSSGRVLLDGRDITGIRPDEMGPLGLSYVPQTDNIFRHLTIAENLA
                     LVLRRVPDRQSRLEELFALFPPLAQKLTDRAGSLSGGQRQMLAVAMALATRPGVVLMD
                     EPSAGLSPKIAAEVLELARSLTTRGVTVLLVEQNVRQALRVADHCYILAEGRNQVDGP
                     AAAILADPVVGEIYLGGRRPGAA"
     gene            complement(11669..12436)
                     /locus_tag="PK812_04460"
     CDS             complement(11669..12436)
                     /locus_tag="PK812_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ68841.1"
                     /translation="MSDPVLSLSGLTRDFGPFRAVDSVSMDIRRGSITGLIGPNGAGK
                     TTLFNMVAGLIAPTAGTVRLNGADITGLPPETLFRRGLARTFQIPRPFRRMSVLDNVL
                     LAPPDQTGESVTGALFRRKTVAEEEVRLLEKARSILDFVTLGALADHPAGKISGGQMK
                     LLELARALMGDPAVILLDEPAAGVNPSLTRILVERIEELNRQGVTFVIIEHDMDFVMR
                     HCDPVIALAEGKVVFQGTVAEAQANPLLLEAYLGTAA"
     gene            complement(12433..13506)
                     /locus_tag="PK812_04465"
     CDS             complement(12433..13506)
                     /locus_tag="PK812_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008196872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRJ68842.1"
                     /translation="MTVETADLRPGHRISRIIKGGWQLAGDHGEVLPEQAIADMEAFL
                     DAGITTFDCADIYRGVEEMIGAFIGDVRRRRGKDVADRVVVHTKLVPDLGRLADIRPD
                     EIEAIVDRSLARLRIERLPLVQFYWWDMSTGDPLAALSVLKDCQRKGKIATLGVTNWD
                     FPTMERFAGAGYDIASAQVQYSLLDRRPAGDFSRWCAARNVQILCYGTLAGGFLTRHW
                     LGRPDPGFSFENRSLIKYRLIIDEFGSWELFQALLETLQAVGDRHGVSLASVATRWVL
                     DQPQVGAAIVGARYARHLPQTLEAFGLALDAADRAAIAAVLAQARGPAGPVYGLESDR
                     SGRHGRIMKYNLNANPNDKVLGA"
     gene            complement(13503..13877)
                     /locus_tag="PK812_04470"
     CDS             complement(13503..13877)
                     /locus_tag="PK812_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008197356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04076 family protein"
                     /protein_id="HRJ68843.1"
                     /translation="MENPAERGFWLYDLRVETVLDGRTPVCRHVEGEFFRVIGENLVF
                     DGSQKVSMYALSAVLPHLPARQRETDPNDWMSTDSEIACADPHCGARFRIVREGRRWF
                     SHASTTGLPEARGTPYWQEDGK"
     gene            13958..14632
                     /locus_tag="PK812_04475"
     CDS             13958..14632
                     /locus_tag="PK812_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008197034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRJ68844.1"
                     /translation="MNAPLIARLDPAKLRENAYFALRDAFTRGAFAPGDQVSLRSLAD
                     QLGVSMTPVREAVRRLVAEGALIDTPSRTLMVPPFDERRAIDLLAARLALEHLVLDQA
                     MDRMDEACVAALTAIVRRAKDGPQAIPDLVANHEFHFTLYRQSGSEVLLPLIEALWLQ
                     YGAYLNLIIKRPDIGRIVEHAHHFEIIDALGKRDRAAAHRALEADVTRSFRFLIPTGW
                     HHDRKP"
     gene            14616..16073
                     /locus_tag="PK812_04480"
     CDS             14616..16073
                     /locus_tag="PK812_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008195111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRJ68845.1"
                     /translation="MTGNPDRIRLGDGLEISRILTGLWQVADIEKEGRTIDPEVGAGW
                     LSAYVEAGFDTFDMADHYGSAEVIAGRLLARGGTPRPRILTKWCPPPGPMTAEVVRAG
                     VEERLTRLGVDRIDLLQFHWWTFEHPAWLDALHELARLREKGLIGELGVTNFDAAHFN
                     LALSDGVPLRTNQVSFSLMDRRAAGELSEVCERHGAWLLAYGTLNGGFLSERWLGKPE
                     PGDITDWSKMKYHRFIRAAGGWEAFQTVLKAATEIARKHGVSVSNVATRWVLQQRRVA
                     GIIVGARLGEAMHAEDNRRLVSFTLDEADCALLDRAFAATTPIPGDCGDEYRRPPFLT
                     ASGDLSHHLDQIEKPFRRHPAMSGAERVLTGSSWEDIAGYCRAQRIGDRILVSGTTAV
                     AGLSRAVAPADAGAQTTYILDRIIASVTSLGGRVEDIARTRIYITDEADVLAISRAHG
                     RVLGAIKPANTLVRVAGLIGDHRVEIEAEAIVRPA"
     gene            complement(16130..>16968)
                     /locus_tag="PK812_04485"
     CDS             complement(16130..>16968)
                     /locus_tag="PK812_04485"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRJ68846.1"
                     /translation="PAISRLMTDLEAAAGFALFERAGRNTMPTPRARLLLAEVERTLL
                     GLSHLETVFQTLRQGGEGRMSIALPPALLPAFADQLLGPFARLHPDASIAVEVVASFQ
                     TFNPKGLRQHDLCVTFEPLGLEGFEEIALAQARAVCLVPAGHPCARRKTPVALRELTG
                     DPFISYWPDAGFRLDVDRLFAAAGLERPLRYEARTTAAVCELVATLGGVSIIPLAGPD
                     IRADRRLAALALEEAPVSAIRFLRPLGALSPLAQAFLAFATTRGLDFERYVSAPTPPL
                     HA"
BASE COUNT         2897 a         5641 c         5589 g         2841 t
ORIGIN      
        1 cgcttccgct cgcggtcaag cgggtctgcg tgctgcgcac cgtcgcggcg ccgccgcagt
       61 tcgaacaggt cttcggcgtc gccaacgcgg attgcgcggg acggcaggag attggcggcg
      121 ccttccggat caccaccggc atcggtgact ggccgggcga tgtcgcgggc tggaacgaag
      181 cggcgcttcg gccgtcgctg gcgattgtcg cggaactgat cgcgcgcggt tccaccattc
      241 tgccggtgct gtccgggctg gggctcgaac gggtctgggg cgggctgatc gacctgaccc
      301 ccgatgcgct gccggtcctg gatgcgccgc atggctgccc cggtcttgtc gtcggcgcag
      361 gattttccgg ccacggcttc gggatcgccc cggtgagcgg cgaaatcctg gcgggccttg
      421 ccaccgggca gtcggtgcca tgggaccttg cgccgttccg catcgaccgc ttttctcccg
      481 ggcgcgcctt cgcgccgctt tcactgcatg gatgaccccg aatgctggac cccaagggac
      541 gcgtgatcct ggtttccggc gcaagccgcg gcatcggacg tgccgtcgcc gagacgctgc
      601 atgcccaagg ctatgccctg agcctcggcg tgcgcgatcc ttcaaaggcg gcggcggcgc
      661 tgtccgctct ggctggagag cggctgcatc tggcccgttt catcgccgag gactggacca
      721 gccatgcccg gtggatcgcg gcggcggcag agcgcttcgg caggatcgat gggctcgtca
      781 acaatgccgg cacgaacagc cagatgacgc tgcgaacgcc ggatgaggcc gaactcgatc
      841 gcgtctgggc cgtcaactgc aaggcgccgc ttaacctgat ccatcacgcg ctgccacatc
      901 tcgaagccag tggggcaggg cggatcgtca acatcgcctc gttgtccggc aagcgggtcc
      961 gcaatgatcg ggtcgcctac aacatgtcga aattcgcgct gatggcgctg acgcatagcg
     1021 cccggcgcat cggctgggac aagggcgtgc gcgccaccgc catctgcccg tccttcgtca
     1081 gcaccgacat gtcgctcgtc gcgagctttc cgtttgatca gatgacgacg ccggggagcg
     1141 tcgccgagct ggtggcggcg gtgctcgcgc tgccgaacaa cgcggccatt gcggaagtgc
     1201 ttgtgaattg ccggcttgag gatacgttgt gagtcaacgc gactgagatc gcacccgtgg
     1261 gaggacctga gatgaagacg acttggcttg cgagcgccac gattgcggcg ctgctgtgcg
     1321 cgacgcccgc attggcgcag aagacgacgc tggtcgtcgg catgcagacg caggacgccg
     1381 gcgtcctcga cccgcatctc aattcgggca caccgggcaa ggcgctgatg aactggatgt
     1441 tcaacggcct cgtgcgcatc aagccgggcc agtcctcgcc cgaccacata gagccggaca
     1501 tcgccgagag ctggacctcc tcgcccgatg gcaaggagtg gaccttcaag ctgcgccagg
     1561 gcgtgcagtg ccatcacgat cttggcgagt tcacatctga ggacgcggtc ttttcgctga
     1621 agcgcgccgg cacgaaggac acctcggcct tctcctcgga ctacgccgcg ttcgacaagg
     1681 tcgaggcgat cgacaagtac acggtgaaga tcacgctcaa gaacgccatt ccgagcctgc
     1741 tcgggcaggt gatgagctat cacggcggca acatggtctg caaaaaggca gccgacggcc
     1801 cggacaagga cggcttcgcc aagaaacccg ccggcacggg gccgttcgtc ttcgccgagt
     1861 atgtgcccca gcagtacgtg aagctggtcg cgaacaagaa gtactggcgc ggcgaaccga
     1921 agatcaagga aatcatctac cgcttcattc cgtccgatgc cgcgcgcgat ctggcgttgc
     1981 aggctggcga gattgacctc atgagcggcc ggcaggagca ggcctgggtg gaccgcatcg
     2041 cccggacgcc cggcctcaag gtgctgacct tcgggccggc ggaaatgtcg gtcatccact
     2101 tcaacatgac gcagcccccg ctggacaaca tcaaggtccg tcaggcgatc gcccatgcca
     2161 tcgaccgcgc cggcatccat cagttccgcg gcatgtcgat ctcgcggccg gcagtctcgg
     2221 tcgtgccttc gggctatttc ggaacgcacg aaaccgcacc gctgctgccg catgacctga
     2281 acaaggcgcg ccagttgctg acggaggccg gcttccccaa cggcatcacc atcaaggcga
     2341 tccataccac cctgccaggc atgatgagct tcatggaagc gacgcaggcc caactcaagc
     2401 gcgccggcat cacgctggac atcacgccgg tggagcatcc caccttccac cagcagatcc
     2461 ggcaggacct ttcgcaggtc gtgcacttcc aggcggcgcg tttcccaatc gccgatgttt
     2521 acctcacgca tttcttccat tcgagttcga tcgtgaagac cccgaccgcc gtgaccaatt
     2581 tctcccactg caaggtggcg gatgcggaaa tcaccgcggc gcgcaccgag cagaacaagg
     2641 agaagcaggt ggcgctgtgg aagaccgcgc aggaaaagat catcgccgag gtctgcgccg
     2701 tgcccgtcaa cgaaatgctg atgaactggg cccatcgcga caacctcgac cttggctatg
     2761 aactgaaagg ctcgctcaac ctcggtccgc cgatcacaga gaacacgcgc ttcacgcgct
     2821 gatcgggcgc cgggcggatc gttaggtcat ccgcccggct gtcttgcgga agcgatggtt
     2881 ccatgggtgc ctttcttgtc aaacgcctga tgttctcggc cgtgacgctg ttcgcggtcc
     2941 tgacattggt cttcatcgtg atccgcatcg tgccgggtga tccggcgctg gtgatcctgg
     3001 gtgatcaggc cgacgcggcg gcgatttccg cgttgcagaa gcggctcggc atcgaccggc
     3061 cgctgttcga gcagtacctc agcttcctgt ccggcgcggt tcggggggac tggggcgtgt
     3121 cgctcgtcac cgggcgtccg gtgctcatgg aagtgctcgc cgtgctgccg tggacactgg
     3181 agcttaccgc cgttgccctg ctgctcgggg cgatgatcgg cgtgccgctc ggcgcctggg
     3241 cggcgatcaa ccgcaaccgc atgccggatt acctcacccg gctgttgtcc ctggtcggcc
     3301 tgtccttccc gcccttcgtg tccgccattc tgttgctgat cggctttgcc ctggcgctgc
     3361 gctggttccc ggtgatcagc agcaaccgcg cgacctggtg ggactggttc cagtcgatcg
     3421 cgctgccggc gctcaacctt gccctcatca tggcggccta catcacgcgc gttacccgct
     3481 cagcgatgct ggaagtgctg caggaggact atgtgcgcac cgcccgggcc aagggcgtgc
     3541 cttgggcggt gatcgtctgg cggcatgcct tgcggaacgc gctcattccg gtcgtcacgg
     3601 tggtgggtct ttatctgtcc atcctgatcg gcaattcggt gctcaccgag atcgtcttca
     3661 accggcccgg actgggcaag ctgatcatcg gcgcgctcaa ccagcgcgac tacatgatgc
     3721 tgcagggcat gatggtgatc tacacgctga tcgtcgtggt cgtgaacatg ctgaccgata
     3781 tcgtctatgc cgtcgtcgat ccgcgggtga agctgtcatg agcgggtcga tccatgatgt
     3841 gcccgacgcc gcgccggagc gcctgcgctg gttgaaggcg acggtttccg ccttcaacgc
     3901 caacaagacg tcgtggttcg ggctcgttgt ttttgcgatc gtggtggtgc tggcggtgct
     3961 ggcgccctgg atcgcaccgc acgatccgct tgagcagaac gtcttctcca aactgcgacc
     4021 gccgttcgag acctactatc tcggcaccga ctatttcggc cgggacatcc tgtcgcgact
     4081 gctccacggc gcgcgcatct cgctggtcat cggcatcctg gccaccatcg tcgcgctggt
     4141 cgtggggtcc atgatcggca tcgtcgccgg ctggtatggt ggccggctcg acatcatcgt
     4201 gatgcagacc atggatatgc tgctggcctt tccggcgctg atcctcggcc tcatcctcgt
     4261 ggccatgctt ggtccctcga tcggcaatat cgtgatcgcc atcgccatca cctcgatacc
     4321 gtccttcgcg cgcatcgccc gcgcgcccac catcgcggtc aaggaacgcg aattcatcga
     4381 tgccggccgc tccctcggtt tttccgacct gaggctgatg gcgcggcatg tgctgccgaa
     4441 catcttcccc gaaatcctgg tgatggggtc tctgtggctg gccagcgcga tccgcaccga
     4501 ggcctcgctc gccttcatcg ggcttggggt gaaaccgccg atccccacct ggggcggcat
     4561 gatccgcgag ggcttcgaga atatcctgga aagctactgg ctggtgctgg cgccgagcat
     4621 cgccatcctg gtcgtggtct ttgcgctgaa cattctcggc gacggccttc gcgatgccat
     4681 cgaccccaag ctgaagggtg agcgatgagc gccccgctgc tcagcgtcga aggtctgcgc
     4741 accgcgttcc ggagcggggg cgaatggcgc gatgccgtcg atgacgtgag cttcacgatc
     4801 aatgccggcg agacgctggc gattgtcggg gaaagcggat cgggcaagag cgtcaccgcc
     4861 ctgtcgatca tgcggctgat ctcgtccatc aacggccgca ttcagggatc ggtccggctc
     4921 gaaggtcgcg acctgctcgc cctgccggaa gaggagatgc gccgcgtgcg cggcaacgcc
     4981 atctccatga tcttccagga gccgatgacc agcctcaacc ccgtcctcac ggtgggccgg
     5041 cagatttgcg aagcgttgta ctatcatcgc gggatggacg cccgcgcggc cgaggccgag
     5101 gcgatccgca ttctcgatcg cgtgcgcatt ccagcctcgc gccagcggtt cggggattac
     5161 ccgcatcgcc tttcgggcgg catgcgccag cgcgtgatga tcgccatggc gctcgcctgc
     5221 cggccgaaga tcctgatcgc cgacgagccg acgacggctc tcgacgtgac gatccaggcg
     5281 caaatcctcg acctcatcaa ggcgctgcaa gaggaagagg ggatggccgt cctcttcatc
     5341 acgcatgaca tggcggttgt ggcggagatc gccgaccgcg tcgccgtgat gtggaagggc
     5401 aagaagctgg aagaccggcc ggccgccgaa ctcttcgccg cgccccatca cggctacacc
     5461 aaggcgcttc tggcagcggt gccgcggctc ggccagatgg agggccatga acgcccgctt
     5521 cgctttgccg tgatcgatcc cgggaccggc gtcacgcagg ttccggcaac ttcggtgccc
     5581 gataccatca gcggcgagac cacgccgctg ttgcaggtca gcggcctgac gacgcggttt
     5641 cccatccgca agggcgtgct gggtcgcctt gccgggcgcg ttcatgccgt cgagaacgtc
     5701 tccttcgacc tgcggaaggg cgaaacgctc gcgcttgtgg gcgagtcggg ttgcggcaag
     5761 tcgaccaccg gtcgcacgat cctgcgtctg gtcgagccga cgagcggcga cgtccgcttt
     5821 ggcggaaccc tggttggcgg tctgccgcgc gatgatctcc aggtcatgcg acggcgcatg
     5881 cagatgatct tccaggatcc cttcgccagc ctcaatccgc gcaaggcggt gggggcggcg
     5941 attgccgagc cgatggttgt gcacggcgtc gccgacgcga aggaggccga ggagcgcacg
     6001 gcatcgctgc tcaggcgcgt cggcctgttg cccgggatgg cgtatcgcta cccgcacgag
     6061 ttctccggcg gacaacgcca gcggatcgcc atagcccgcg cgctcgcgct ctcgcccgaa
     6121 atggtggtgg cggacgaagc ggtctccgcc ctcgacgtct cggtgaaggc gcaggtactg
     6181 aacctgatgc tcgacctgca ggccgagctg ggcctcgcct atctcttcat ctcccatgac
     6241 atggcggtgg tggagcgggt aagccatcgc atcgcggtga tgtatctggg cgaaatcgtc
     6301 gagattggcc cgcgcgctga agtcatgcgc aacccggtgc acgcctatac ccgcaagctc
     6361 ctgtcggcgg tgccggtgcc cgatccggcg cgccggcgca agcgcggcac cgtcgctgtc
     6421 gaggagctgc caagccccat ccggccgccg gactggcgcg cgccgcccac ccgcatggaa
     6481 gcggtcgggc cggatcattt cgtgcaggtg gtggaagggt agggtccatc cggaccttgc
     6541 accgatgcgg gatcgtgccg gaccgcgcgt ggcacgtgtc cggccttttc aatggaacgc
     6601 gatcggacgt ccttgattcc cgcgctggat gccggaagat ggcgcgaccg cgcatgatcc
     6661 cgcgcgccag tccatccgac tacgccagga cgctttgaca tggccgatcc ctatcccgcg
     6721 ccggacgcca cgccaccgct gcgtgtcctc attctcggtg cgtcgggacc gggctggtac
     6781 gccgcctcgc aacaggagcg cgcggagcga atcctgcccc gtcttgccgc tgtcttcgcc
     6841 gcctggacgg cgctgggcgc ccggctgatc gtcacgctgg acgacgacct gttgaaggtg
     6901 gggcaaccgg ccgaccacga tcacacctgg tatctggtct acgatgtgcc cgatctgcag
     6961 accgtatcga ccatgctgca ccagttccgc ctggaggtgg acggtgcgcg cctcgaccgc
     7021 tggttccggc tggaagcaaa ggtttgccgg ccgttctttc cggcggaagc ggtggctcag
     7081 ccgtagcgca ggtattgccc gcgcgtttcg agcgcggcga tcagcgcctg cgtcaccgga
     7141 tgcggccctt cgctgaaatc aacgctggtg acgatggtct ggcccggcac ttcgcagcgg
     7201 gcgcggatgc aatgaggacc gctcaccggc gaacagtgca gggcaacgct ggtggcgtcg
     7261 attccaggtc cagccaacgc ggcggctgtc gccgtgttga ggacgtcggg atagcgcgcc
     7321 gcggcggcgc gcaacgatcc cgaaaacacg aggcccgggg ccggtccaag gcgcggcgcg
     7381 tgctgttcga catgaaggcg agcgccggaa cccgccgcgc acagggcagg gccggcgatc
     7441 cagccggtga acaggcgcag ttcgtggcgg tgctggcgtg cggtttccag caggtgggcg
     7501 cgcagatcat cgtcgacaag cgccgcggcg cccaccgacc acagatcgcc ttgcgcgagc
     7561 agggcggttc cgaacgcgcg caaggcctcc gggccggcgg catcgatggt gagcggggcc
     7621 gccgaccagc cgccgccgcg acggatgacg gagttcaggg tgtagcggtc ggaacgagcg
     7681 agccagtcgg caaggcctgc accgatcgtc ccgtgcccga tcaggttgac cggtgtcatg
     7741 gccggtccgc acgcgagagg ccggcctccg cagaggccgg cccgatgccg cgtcgatcgc
     7801 tcacttcacg gagctgatga atgcgccgac ctcctcaagg gtgatgtagc gcttctcctg
     7861 cacgccactg gcgccgaagg tccagagcac ggcgggcgcc gagacgtcgc cgttcttgtc
     7921 gaacgcgacg gcgcccgtgc cgccctggaa ggacacggtc ttgccctgct tcaggaggcc
     7981 cagcgccttc ttgaagccgt cgacatcgcc gttgaccttc tcgcctttgg gatcggtgac
     8041 catgccgacc ttggcagcga tctgcgcgcc ggtcgcattg cggccggctg catggtaggc
     8101 gagaagcgag atggcggccg cgtcgaagct gttcggcagg cccggaccgg caggcgcctt
     8161 gccgaatttc gccgtgaagg ccgcaacgaa ccccttggcg ctgtccgtgc gcggagtgga
     8221 agtgtccgtg ccgacgaagt tcgccagatg ctgcatgccg acggtgttgc ggaagtcatc
     8281 cgacttcagc gcattcgcgc cgatcatgtt ggtcgtgccg ccgagcgaca gccactcgcg
     8341 caccacggcg atgccttcct tcggatacag cgcgagatag accgcatcgc ccttcgcggc
     8401 gagcgcgcgc gccacttcgg cgcgatagct cggctgaccg tcggtgatgg cgatctgcgt
     8461 tgtcacctcg atgccggact tggcgaaggc gttggctgcg atcttggcga tgtcctggcc
     8521 ccagtcgtcg ttcttgaaga agatcgtcac cttcttgtag cccgcttcct tggccgccat
     8581 cgccgcgacc gcgccctgca cgctggaggt cgcgaaggtc ctgaaccaca agcccttggt
     8641 cgcgccttcg ttcgccatcg cggtcaggcg ggtcgaagat gaacagcacg acatctgcat
     8701 cgttccggcc ggaaccgaga ccgaggtcag gatcgggatg gtgatgccgg aggcgaccgt
     8761 accaagcaac agctggacct tgctgatgtc gaccagcgcc tttgccgcat cgacgccgac
     8821 cttcgcgtca ttctgatcgt cgcgcaggat cgtctcgacc ttgcagccat cgacgccgcc
     8881 ggcctggttg atcatgtctg ccacccagag cgcggtttcc gcgatcggct tgccccagtc
     8941 gaccgaggtt ggaaagatgg cacccacctt gaccgaacac tcttgtgccg cagcgccgcc
     9001 agccggcgcc agaagcgcca tcgccgcgcc ggccatgctt cccaaaatcc gtagtctcat
     9061 gtcagttccc tccattgtcc gcggttgtgg cccctgaggc ccggtgaccg gaacgcgcaa
     9121 tccgttccgg caggatcccc tgcggccgcc acagcagcat gccgacgatc accgatccga
     9181 tcagaatgta ttgaatcggg ccggtgtaaa gctgcattgc ttgtggcgcg aaggcggcga
     9241 gcagaaatcc gctcgccgtc caggccgccc agattagata agctccgaga atcgctccca
     9301 gattgttgcc ggcgccgccg acgatcagca tcgcccagac ctggaaggtg aggaccggcg
     9361 ccaggtcctg cgggctgacg aaggcataga aggcgacgta aagaccgccc gccgcgccca
     9421 tgatcatcga tccgatcacg aaggattgca ggcgtaggcg cgccgggttc ttgcccagcg
     9481 actgcgccgc gatttcgtcc tcgcggatgg cgcgcagggc acgtccgtag ggggaatgca
     9541 ccagccgttc gaggccgaca tagaccgcca gcaacacagc catcaccagt gtcaggaaga
     9601 acagcccata aaggaagttg ttgccgatgg cggcttccag cggtcgcgga aatccgcgca
     9661 ggcccttcgc tcctcccagc agccactccg cgttccgcgc gacgctttcg aacgccaccg
     9721 ccacgccgaa ggtcgcgatg gcgagatagt cgtgccgcag cctgagagtc agccggccga
     9781 agaaccaggc cgccagcccg gcgctggcga tgcctccggc gagcgcgatc acgaacggca
     9841 gctggaaccc gccgaactgc ccgggctcgg ggcgaccgcc gaggatcatc gtcacatagg
     9901 cgccggcgcc gaaaaatgcg gcaacgccgc cgttgaacaa cccggtgtag ccccactgca
     9961 agttgaggcc gagcacgacg atgccgagaa tgccagccat cgtggcgaag aacacgagat
    10021 aggaaaccac cccgctcatg ccaaatcttc cccgaacagg ccgtgcgggc gcaccagcag
    10081 cacgccgagg atgattagaa acggcatggc tggcacgtag ccggacggca gcaccatcag
    10141 cgacaggcct gacgcgatgc cgacgatgaa cccgccgagc accgcaccgt agacgctgcc
    10201 gatgccgccg acgatggtgg cggcgaagac cggcagcagg aagttgtggc ccagcgccgg
    10261 cgacaggtga ttggtcaggg cgtagaagac gcctgctgcg gcggaaagtc cgcctccgat
    10321 gagccagaca gcggcgatca tggtggacag gcgcacgccc gccacctgcg ccagcgccgg
    10381 attttccgcg acagcccgca gcgcgaagcc gaaggtcgtg cgtgtcagca ccacatgaaa
    10441 cgccgccatg agcgccagcg ccgccgccag cacgaagacc tgatccggtt tgaccagcac
    10501 gagcggatca aggctgagca cgaccgcgaa ggcgatgtct ggtgaataga gctgcgcatt
    10561 gtgcccgaaa acgagcgtga tgacgttccg gatgatcagg gccacgccga aactggcgaa
    10621 gaccatcgac aacgcgtcgg ccttggcgcg gattcgcttg aagatcagcc agtcgatgag
    10681 cactgccgat gcgccggtga ttgccatggc aagaagcgtc gcaagcgtca gtgacgcgga
    10741 aagcgtcagc ggcttgaacg cggtaccgag cgcgggaacc agtgccgcga acagccggtc
    10801 gaacaccagc gccgcatagg cgccgatggc cagcagttcg gcataggcga agttggcgaa
    10861 gcgcagcatg tgcatgacca tcgtcaggcc tatggcgccg agcgagatga ccgcccctgt
    10921 caggataccg tcgagcaggc tctggatcat gccgcccccg ggcgcctgcc gccgagatag
    10981 atttcgccaa ccacgggatc cgccaggatt gcggctgcgg ggccgtccac ctggttgcgc
    11041 ccctcggcga ggatgtagca gtggtccgca acgcgcagcg cctgccgcac attctgctcg
    11101 accagcagca ccgtcacgcc ccgggtcgtc agggagcgcg cgagttcgag aacctccgcc
    11161 gcgatttttg gcgagagccc ggccgagggc tcgtccatca gcacgacgcc ggggcgtgtc
    11221 gccagcgcca tggcgacagc cagcatctgc ctttgcccgc cggacaggga gccggcccta
    11281 tccgtcaact tctgagccag cggtggaaac agggcgaaga gttcctccag ccgggactgc
    11341 cggtccggca cgcggcgcag cacgagcgcc aggttctcgg caatcgtcag gtggcggaag
    11401 atgttgtcgg tctgcggcac atatgacagg ccaagcggcc ccatctcgtc cggtcggatg
    11461 ccggtgatgt ccctgccgtc gaggagcacg cgcccgctcg acaccggaac aaggccggcg
    11521 atggccttga tcagcgtcga cttgccggcg ccgttcggcc cgatgatcgc ggtcaggctg
    11581 gcgcgttgag cgctcaggtt cacctgccgc aggatcggca gatcgcggac ataaccggct
    11641 tccataccct cgatccggat cgccgcgctc atgccgccgt ccccagatag gcctcgagca
    11701 gcagcggatt ggcctgcgcc tcggccaccg tgccctgaaa caccaccttg ccttcggcca
    11761 gggcaatcac cggatcgcag tggcgcatga cgaaatccat gtcgtgctcg atgatcacga
    11821 aggtgacgcc ctgccggttc agttcctcga tccgctcgac caggatgcgc gtgagcgacg
    11881 ggttcacgcc ggccgccggc tcatccagga ggatcacggc gggatcgccc atcagggccc
    11941 gcgcgagctc cagcagcttc atctgcccgc ctgaaatctt gccggcggga tggtccgcca
    12001 gggcaccgag cgtgacgaaa tcaaggattg aacgtgcctt ttcgagcagc cgaacttcct
    12061 cttcggccac cgtttttcgg cgaaaaaggg cgccggtgac gctttcgccg gtctggtccg
    12121 gcggcgcgag cagcacattg tcaaggacgc tcatgcgccg gaagggccgg gggatctgaa
    12181 aggttcgggc aaggccgcgc cggaacaggg tctccggcgg caggccggtg atgtcagccc
    12241 cgttcaggcg gactgtcccg gcggtcggcg cgatgaggcc ggcgaccatg ttgaacagcg
    12301 tggtcttgcc ggcgccgttt ggaccgatga ggcccgtgat gctgccgcgc cggatgtcca
    12361 tgctcacgga atctacggcc cggaacggac cgaaatcccg cgtcagcccg ctgagcgaca
    12421 gcacggggtc gctcatgccc ccagcacctt gtcgttggga ttggcgttga ggttgtactt
    12481 catgatccgg ccgtgccggc ctgaccggtc tgactcaagt ccatacaccg gtccggccgg
    12541 tcctctcgcc tgggccagaa cagcggcgat cgccgcacga tcggcagcgt ccagcgccaa
    12601 tccgaacgct tccagcgtct gcggcagatg ccgggcatag cgggcgccga cgatggctgc
    12661 ccccacctgc ggctgatcca gaacccagcg cgtcgccacc gacgcgagtg acacgccatg
    12721 ccggtcgccg acggcctgca gggtttccag cagcgcctgg aaaagctccc agctgccgaa
    12781 ttcgtcgatg atcaggcgat acttgatcag actgcggttc tcgaacgaaa atcccggatc
    12841 aggacgccct agccagtgcc gcgtcaggaa accgccggcc agcgtgccgt aacacaggat
    12901 ctgcacgttc cgcgccgcgc accaccggga gaaatcgccc gccggtcgcc ggtcgagcag
    12961 ggaatactgc acttgcgccg aggcgatgtc atagcccgcg ccggcgaagc gctccatggt
    13021 cgggaaatcc cagttggtga cacccagtgt ggcgatcttg cccttgcgct ggcagtcctt
    13081 gagcaccgat agcgcggcca gcggatcgcc cgtgctcatg tcccaccagt agaactggac
    13141 gagcggcaac cgctcgatcc gcaggcgcgc caacgaccgg tcgacgatgg cctcgatctc
    13201 gtcgggccgg atgtccgcga gccgccccag gtcgggcacg agcttggtat gcacgacgac
    13261 gcgatcagcg acgtccttgc cgcgccggcg acgcacgtcg ccaatgaagg cgccgatcat
    13321 ctcctcgacg ccacgataga tgtcggcgca gtcgaaggtg gtgatgccgg cgtcgagaaa
    13381 ggcttccatg tcggcgatgg cctgctccgg cagcacctcg ccgtgatcgc cggccagttg
    13441 ccacccgccc ttgatgatcc gcgagatgcg atggcccggc ctgagatcgg cagtctcgac
    13501 cgtcatttcc cgtcctcctg ccaataaggc gtgccacgcg cttccggcag gcccgtggtc
    13561 gatgcatggg agaaccagcg acgaccctcg cggacgatcc ggaaccgcgc gccgcaatgg
    13621 ggatcggcgc aggcgatctc ggaatcggtc gacatccagt cgttcggatc ggtctcacgc
    13681 tgccgcgccg gcaagtgcgg caggacggcc gaaagagcgt acatcgaaac cttctggctg
    13741 ccatcgaaaa ccagattttc gccaatcaca cggaagaatt cgccctcgac atgccggcag
    13801 accggcgtgc ggccatccag caccgtttca accctcagat catagagcca gaagccgcgt
    13861 tcggctgggt tttccatagg gctgcgttcc gacttgaccg gcctattgtg atctgtgata
    13921 ttttatctgt gatgctatgg agcctgtcaa gccagccatg aacgcgccac tgatcgctcg
    13981 tctggacccg gcgaagttgc gcgagaatgc gtattttgcg ctccgcgatg cgttcacgcg
    14041 gggcgccttt gcgccgggcg accaggtcag tttgcgttcg ctggcggacc aactcggcgt
    14101 ctcgatgacg ccggtgcgcg aagcggtgcg gcgcctcgtg gcggaagggg cgctgatcga
    14161 cacgccgtca cgcaccctga tggtgccgcc tttcgacgaa cggcgcgcga tcgatctgct
    14221 ggccgcgcgc ctggcgctgg aacatctcgt gctcgatcag gcgatggacc gcatggacga
    14281 ggcctgtgtc gctgccctga cggccattgt ccggcgcgcc aaggacggcc cgcaggccat
    14341 accggacctc gtagccaacc acgagttcca tttcacctta tatcggcaaa gcggctccga
    14401 agtgctgctt ccactgatcg aggcgctctg gctccagtat ggcgcctatc tgaacctgat
    14461 catcaagcgc cctgacattg gccggatcgt cgagcacgcg catcatttcg agatcatcga
    14521 tgcgctcggg aagcgtgacc gcgcggcagc ccaccgcgcc ctggaggccg acgtcacccg
    14581 ctcgttccgt tttctcattc ccaccggatg gcaccatgac cggaaaccct gatcgcatcc
    14641 gcctcggcga cggccttgag atttcgcgca tcctaaccgg gctctggcag gtcgcggaca
    14701 tcgagaagga aggcaggacc atcgatccag aggtcggggc aggctggctg tccgcctacg
    14761 tggaggccgg gttcgatacc ttcgacatgg ccgaccacta cggcagcgcg gaagtcattg
    14821 ccgggcggtt gctggcgcgg ggagggacgc cacgcccgcg catcctgacc aaatggtgcc
    14881 cgccgccggg tccaatgact gcggaggtcg tgcgcgccgg tgtcgaggaa cgcctgacgc
    14941 gccttggcgt cgaccggatc gacctgctgc agttccattg gtggacgttc gagcatccgg
    15001 cctggctcga cgcactgcac gagttggcgc gcctgcgcga gaaagggttg atcggcgaac
    15061 tcggtgtcac caacttcgac gcggcgcatt tcaatctcgc cctgtcggac ggggtcccgc
    15121 tgcgcaccaa ccaggtcagc ttttcgctga tggaccggcg cgcggccggc gagctgtccg
    15181 aggtctgcga gcggcacggg gcctggctgc tggcctatgg aacgctgaat ggcggctttc
    15241 tgtccgaacg ctggctcggc aagccggagc ccggcgacat caccgactgg tcgaagatga
    15301 aataccaccg cttcatccgg gccgccggcg gctgggaagc gttccagacc gtgctgaaag
    15361 cggcaaccga gatcgcgcgc aagcacggcg tctcggtctc caacgtcgcg acgcgctggg
    15421 tcctgcaaca gcgacgggtc gcggggatca tcgtcggagc acgcctcgga gaagcgatgc
    15481 atgccgaaga taaccgccgc ctggtctcgt tcacgctgga tgaggccgat tgcgcgttgc
    15541 tggaccgggc cttcgccgcc acgacgccca ttcccggcga ttgcggcgat gagtaccgca
    15601 ggccgccatt cctcacggcg tccggcgacc tttcgcacca tctcgaccag atcgagaagc
    15661 cgttccgccg gcatcccgcc atgagtggcg ccgaacgcgt gctgaccggc tcgtcctggg
    15721 aagacatcgc cggctactgc cgggcccagc gcatcggcga ccggatcctg gtctcgggca
    15781 caacggccgt cgcggggctg tcgcgcgcgg tggctcccgc tgacgccggc gcccagacga
    15841 cctatatcct ggatcgcatc atcgcctcgg tgacgtcact gggcggccgg gtcgaggata
    15901 tcgcgcgcac ccgcatctac attaccgatg aggcggatgt gctggcgatc tcgcgggcgc
    15961 atggccgcgt tctcggagca atcaagccgg ccaatacgct ggtgcgtgtc gccggactga
    16021 tcggcgacca tcgggtcgag atcgaggcgg aagccattgt ccggccggct tgacagcccg
    16081 gaagcggtca cacatccggg cgacggtcgc tgtcagggct gcgcggttct caggcgtgca
    16141 acggcggggt cggcgcgctg acatagcgct cgaaatccag gccccgagtc gtcgcgaagg
    16201 ccaggaacgc ctgcgccagc ggcgagaggg cgccaagcgg gcgcaggaac cggatcgcgc
    16261 tgaccggggc ttcctcaaga gccagcgcgg ccagccgtcg atcggcgcgt atgtccggcc
    16321 cggccagcgg aatgatgctg accccgccca gcgtcgccac cagttcacag acggcggccg
    16381 tcgtgcgggc ctcgtaacgc aacggccgct caagacccgc cgccgcgaac aggcggtcga
    16441 cgtccagccg gaagccggca tccggccagt acgagatgaa cggatcgcca gtcagttccc
    16501 gcagggcgac cggcgtcttg cggcgcgcgc acgggtgacc ggcgggcaca agacagacgg
    16561 cgcgcgcctg cgccagggcg atctcctcaa acccctcgag gcccaaaggc tcgaatgtga
    16621 cacacagatc gtgctgacga agacccttcg ggttgaacgt ctggaaggag gcgacaacct
    16681 cgacggcgat ggaagcgtcg ggatgaagcc gcgcgaacgg gccgagcaac tggtccgcaa
    16741 aagccggcag cagcgccggc ggaagggcaa tgctcatccg gccttcgccg ccctggcgca
    16801 gcgtctggaa cacggtttcc agatgcgaga ggccgagaag cgtgcgttcg acctccgcaa
    16861 gcagcagccg tgcccgcggc gtcggcatgg tgttgcgccc ggcgcgctcg aacaaggcga
    16921 agccggcggc cgcttcgaga tccgtcatga gccggctgat ggccggct
//
LOCUS       DAOMXN010000031        16846 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_1941_length_16846_cov_10.056637, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000031 DAOMXN010000000
VERSION     DAOMXN010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 16846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16846
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1941_length_16846_cov_10.056637"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="PK812_04490"
     CDS             complement(<1..850)
                     /locus_tag="PK812_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HRJ68847.1"
                     /translation="MNAPLKLSTPVSHANLANAVRFLAMDAVEKARSGHPGMPMGMAD
                     VATVLFTRFLKFDPAVPSWPDRDRFVLSAGHGSMLLYALHHLVGYADMTTDQLRAFRQ
                     LGAKTAGHPEHGHALGVETTTGPLGQGIATAVGMALAERMLNARFGDALVDHHTYVIA
                     GDGCLMEGISHEAVDMAGCLKLGRLIVLWDDNAITIDGTTALSTATSQPQRFAASGWH
                     VQSVDGHDAEAVAAAISAAKADPRPSLIACKTVIGKGAPNKQGSESTHGAPLGPAEIA
                     AARAAAG"
     gene            complement(874..1749)
                     /locus_tag="PK812_04495"
     CDS             complement(874..1749)
                     /locus_tag="PK812_04495"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HRJ68848.1"
                     /translation="MSIKHPIIAITGSSGAGTTSVKKTFVEIFRREGVSAAFIEGDAF
                     HRYNRAEMRHRMSQAAIDGNNNYSHFGDYANLLEKLQDTFRQYGENGTGETRTYVHDA
                     EEAAEFAVQPGNFTPWRPFPADTDLLFYEGLHGAVVTDKVNLAQHVDLKIGVVPVINL
                     EWIQKLHRDRSARGYSTEAVTDTILRRMPDYINFICPQFGETDINFQRVPMVDTSNPF
                     IARWIPTADESMVVIRFKNPRGIDFPYLLSMIHDSFMSRANSIVIPGGKLDVAMQLIL
                     TPLIMKLVERRRRAL"
     gene            complement(1761..2801)
                     /locus_tag="PK812_04500"
     CDS             complement(1761..2801)
                     /locus_tag="PK812_04500"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019906780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HRJ68849.1"
                     /translation="MDKVQIGTKLEDALSAMAPAPVASVVAAIAQASVPIVSVIRGGP
                     LAGPLGAATGTGNADGDAQKALDVLADRQFSQGLKGSGVRAMASEELDEPLEVDTDGT
                     ILVAIDPLDGSSNIDTNVSIGTIFSLLAAPPDRAPTTADFLQPGVRQLAAGFIIYGPQ
                     TSLVFTTGVGVHMATLDPLSGSYVMTRLSLIIPEGSPEFAINASNYRHWHRPVQAYVD
                     DCIEGADGPRGKNFNMRWIASLVADAYRIFMRGGVFLYPADRRKGYESGRLRHVYEAS
                     PIALLVEQAGGQATNGVNRILDLVPASLHARVPLIFGSSDKVTRIRRYHVEASAAVET
                     SPLFGKRGLLRG"
     gene            2920..3882
                     /locus_tag="PK812_04505"
     CDS             2920..3882
                     /locus_tag="PK812_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRJ68850.1"
                     /translation="MSQLSLRHFETLQAIVRQGTLVGAASYLNVTPAALTARLKALED
                     LVGLKLFDRTSGGMKLTMAGEMALDSAHRIEREAKDFLARMATIRRGQGGRLAVAAVS
                     TAKYFAPRLIAAFTREHPNIDLRFLIGNRDDTIAALQSLSAEVALTGRPPADMPAEKA
                     ALGAHPYVIVAPPFHPLVGERAISKDRLGQETFLFREQGSGTRSIFDYFIGDITLHRA
                     QVGIELGSNETIKQAVMAGLGVALISAHTIAAEVQDGRLRCLDVEGLPIIRQWFVVNR
                     ADREISPIAQAFRAFVTERGAHFLPRLIGGHRDERPLTVLPPEE"
     gene            complement(4061..4366)
                     /gene="cas2"
                     /locus_tag="PK812_04510"
     CDS             complement(4061..4366)
                     /gene="cas2"
                     /locus_tag="PK812_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HRJ68851.1"
                     /translation="MWLFVVFDLPVLTKTERKRATRFRQYLLDEGFSMMQFSVYLRFT
                     SGKEQIEGLTRRIGKKVPNEGKVDVLSLTDQQYAAMTSFRGTTDAARPTKPDQLALF"
     gene            complement(4413..5393)
                     /gene="cas1"
                     /locus_tag="PK812_04515"
     CDS             complement(4413..5393)
                     /gene="cas1"
                     /locus_tag="PK812_04515"
                     /inference="COORDINATES: protein motif:HMM:TIGR03639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HRJ68852.1"
                     /translation="MVSLILGPNDAVCCSKTGRAAMLRRVIEISGEGRRLSLNRGFLS
                     ISGPDGLLGEVPLDDVEAVIAATPSLSYTNQAVAALAERGAPLAICGKDFRPVAWLLP
                     VSGHHAQGVRMEAQASCPRARKARLWAQIVKSKLTAQAEALERTERPSAQLRRLIRAF
                     RSEDAATLEAQAAQRYFPLMFGDGFRRDRNSEGINALLNYGYTVLRAATARAIVAAGM
                     NPSLSLFHGSRGDGLRLADDLIEPFRPTVDLTVLGLAEDGRLELTPGVKRALATVLAS
                     DFRSPAGTGPLSNALVRLAQSLSQAFADPSASLALPDPLIPLQANDGCST"
     repeat_region   5410..6963
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=5476..5511
                     /rpt_unit_seq="gttgcggttggaccgcgccttcgcccctgctagaac"
     gene            complement(7308..10190)
                     /gene="cas9"
                     /locus_tag="PK812_04520"
     CDS             complement(7308..10190)
                     /gene="cas9"
                     /locus_tag="PK812_04520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01865.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HRJ68853.1"
                     /translation="MDPLQLRTTGLDAPLPLIHFGRALFHLNQRRGFKSNRRTDGKNA
                     VSGAMAEGAQRLREAIQTNGARTLGEFLYKRQSEGSPSRFRQAAGATPGKVTYDLYPT
                     RKIVEDEFDALWAAQAIHLPHVLTESARTAIRAAIFDQRPLRPVKPGRCTLVPEEERL
                     PMALPSVQARVVFETLNALRFGEGLNLTQSLDLAQRKVLAQTLMAGKDLSFTLIRKTL
                     KLGSTVRFSLEEGGRDRIEGSKTAKALSRDSGFGKAWQQMQVSKQEQIVRVLLEEDDS
                     DAAVAWVMAETGLDETHAAYVSGVALPEGYGRLGATANALIVQELAADVITYDEAVRR
                     AGEKSGLFQHHSDFRDGEIFPRLPYYARVIERHVGFGSADPDEKDEAKRFGRIANPTV
                     HIGLNQLRRVTNRLIQRFGHPDEIVIELARDLKRSSRQRDEDQRRNTQNRKQNDIRRK
                     KLGELGLPENGLNMALLRLYDEQMAGGVVRCPYTLRVIGVHELFTEAVEIEHVLPFSR
                     TLDDGMANKVLCFREANRKKRNQTPFEAYGATPDWPAIAANAETLPPYKRWRFREDAM
                     ERFDNRERDMLARQLNETRHLARAAKAYLSKVCGDENDVWVVTGQLTALMRRQWGLDS
                     ILGDDNRKNRNDHRHHAIDAATIGCISRSMLQRVAREAARNEETGAARVVAGFGEPFP
                     GFRDRLRDLVRTMIVSHKPEHGKGGALHEDTAYGIVKSERDKAIGNLVHRKPLDSLNP
                     NEIERVRDPDLRAALQQVVHEVTGEGGKPDPKALSAALLAWAKADAERIAARTGKLRN
                     PVRHVRLLKPKANAVPIRDRRTGRPYKAVVPDENWCLDVVSFRDGKGGQIWKAFAASL
                     FEVNQRDWRPLWEREKLGGKLVMRLHKGDLLEIDDKDGVRRVKRVVRLNAAAERLYLV
                     QHCEAGDFQKRHDDPEDPFRWDLAAISGLSVRNAEIVCVDEAGLRR"
     gene            complement(11046..11276)
                     /locus_tag="PK812_04525"
     CDS             complement(11046..11276)
                     /locus_tag="PK812_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68854.1"
                     /translation="MIRLASIKPGVSHKSGRRYFRGRLADGSTVWLIEDHDSPGWTLK
                     LDPHEERPVTIDAKVSSVAKLLPSRTGDDDRS"
     gene            complement(11273..11497)
                     /locus_tag="PK812_04530"
     CDS             complement(11273..11497)
                     /locus_tag="PK812_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68855.1"
                     /translation="MSGNENDGLEVLCRMTCHEPPGRPFARWFHGLTGDGWDYMLAES
                     REAFAPRTVKCCRIDPTQGFTPSAARDRPQ"
     gene            complement(11494..12291)
                     /locus_tag="PK812_04535"
     CDS             complement(11494..12291)
                     /locus_tag="PK812_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68856.1"
                     /translation="MRHTRQAGASRSAPEFRAALIAIEAPPEAPKRVRKGFTTSGKRK
                     PRDLAPIRLRQVNRVLDLRYGGRADRDCVQAVIEVTAHLCHAMDGWPDNFRGWVRERA
                     PTFNPANVAALIDIVSAEPAPWDGAAAGRILRLSEIDRQRLRAWTIEPFDLSPAERAE
                     SRKLRKREADKARRRKAGAKDRAQSAAKAKPWREQGVSRATWYREKAARDAAARETVS
                     RTTYVDKKHRNARRVSVSPDALAKITVTTPAPPADALPARPALEIAA"
     gene            complement(12390..12611)
                     /locus_tag="PK812_04540"
     CDS             complement(12390..12611)
                     /locus_tag="PK812_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68857.1"
                     /translation="MQTPDKLYTVQEALPLMGMRLTRFYALVGAGEIATIKNGRRTLI
                     AEAEILRFRASLPVVGRREQAASDQPRAA"
     gene            complement(12685..13446)
                     /locus_tag="PK812_04545"
     CDS             complement(12685..13446)
                     /locus_tag="PK812_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68858.1"
                     /translation="MSGEWKNSPLACQTFFQAIQTSRAFGRKPDPEVVGFHHWNMARE
                     QGSIDIPLEAIHFCHALIESTIVERDELRLLATAFINGKPIDPESLAGRRCEIALAFY
                     IDIEAKCEDDDTFPHGPFEIAKIHARHRAAAAEIAARAKRRKGEKKGIRHRVYEIVDD
                     FLLVLLAAAKAAGVATKLPSTTSEEGNEFARFIQSAIDTSERVFVALAERRQLPPEVT
                     QKVKLALSDLSGRRLNDRMRRIINEFGQIDWKPPI"
     gene            complement(13612..14850)
                     /locus_tag="PK812_04550"
     CDS             complement(13612..14850)
                     /locus_tag="PK812_04550"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HRJ68859.1"
                     /translation="MALTDTAIRNAKPGAKLRKISDGRGLQLHISPTGGKFWRWAYRF
                     GGKQNALSFGLYPDLSLQAARQRAEEARKLLVLGIDPGLQRRLDKISRIDTEQATFAV
                     VAAELLAKKRCEGRAEMTLAKNEWIFGLTGEAFGKRPLSDIAAPEVLAVLRRVEAKGR
                     LETAKRMRSMIGEVFRYGIATGRASSDPTQALRGALTAPVVKHRAAITEPMAFGGLLR
                     AVDGFQGQPTTVAALKLLALLAARPGELRLAEWSEFDLEKAVWTVPAKRMKMRREHKV
                     PLPRQAVAILAALHALTGRGRLVFPGYGKTAKDGGVLEPKPISENTLNAALRRMGFGP
                     DDMTAHGFRASFSTMANESGKWNPDAIERALAHADQDGIRAIYARGAYWQERVALAQW
                     WADENDRMRQGGKVLKFQAG"
     gene            complement(15024..15099)
                     /locus_tag="PK812_04555"
     tRNA            complement(15024..15099)
                     /locus_tag="PK812_04555"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15064..15066),aa:Thr,seq:cgt)
     gene            complement(15212..16777)
                     /locus_tag="PK812_04560"
     CDS             complement(15212..16777)
                     /locus_tag="PK812_04560"
                     /inference="COORDINATES: protein motif:HMM:NF018875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HRJ68860.1"
                     /translation="MIRMFLFFALVLAAAFGVIQLAETPGHVVLNYGGFEYQVTLLKA
                     AGALVLMTVALMLVWTLLRLLFRLPGILSVATQVRRRAKGYAAVSRGMVSVGAGDRRA
                     AAKFASEAERLLGNEPLTLLLKAQSAQLAGDQQGAEATFNRMLEDPETRVLGLRGLFV
                     EARRNGNNAARSFAEEAFRIAPSAPWASEAVLEYRCADKDWGGAVAAVDQNASRRVVD
                     RDVAKRQKAVLLTAEALHLAERAPDEALDVALRALKLDAGLVPAAALAARRLSARGDY
                     GKATRVIEAAWRVNTHPELADAYLSVRGGDSSHDRLKRARQLVKLTPKDREAQLTLVR
                     AAIDAREFAEARGLLEPLVLERPTARACVLMAELEDKEHNAAGLVRKWLSRASIAPRD
                     PAWVADGLVADAWAPVSPVTGKLDAFVWMTPPQSLEASVRASIDAGHFSQPEPETIAA
                     APAIEAAPSLATAEMEAPKPVSEPVAVVLPPEPETPPAAPGAQPVSPLEHAPDDPGPR
                     RPDETKRGWRLFG"
BASE COUNT         2808 a         5359 c         5407 g         3272 t
ORIGIN      
        1 agccagcggc tgcacgggcg gcggcgatct cggccgggcc gagcggcgcg ccatgggtcg
       61 attccgatcc ctgcttgttc ggggcgccct ttccgatcac ggtcttgcag gcgatcagcg
      121 aagggcgtgg atcggccttg gcggcggaaa tggcggcggc gacggcttcc gcgtcgtgtc
      181 cgtccaccga ttgcacatgc cagcctgagg cggcgaagcg ttgcggttgc gatgtcgcgg
      241 tcgagagcgc ggttgtgccg tcgatggtga tggcattgtc gtcccacagg acgatcaggc
      301 ggccaagctt caggcaaccc gccatatcga ccgcctcgtg gctgatgccc tccatcaggc
      361 agccgtcgcc ggcgatgaca taggtgtggt ggtcgaccag ggcatcgccg aagcgcgcat
      421 tcagcatgcg ctcggcgagc gccatgccaa cagcggtggc gatcccctgt cccagtggac
      481 ccgtcgtcgt ctcgacaccc agcgcatggc catgttcggg atggcctgcg gtcttcgcgc
      541 cgagctggcg gaacgccctg agctgatccg tcgtcatgtc ggcatagccg acgagatgat
      601 gcagtgcgta gagcagcatc gagccatgac cggcggatag tacgaaccgg tcgcggtccg
      661 gccaggacgg cacggccgga tcgaatttca ggaagcgcgt gaacagcacg gtcgcgacgt
      721 ccgccatgcc catcggcatg ccgggatggc cggatctggc cttctccacc gcgtccatgg
      781 cgaggaatcg gactgcattc gccagattgg cgtgcgacac cggagtcgag agcttgagtg
      841 gcgcgttcat gacggttcct ccggggaaag gcttcacagc gcgcgacggc gacgctcgac
      901 cagcttcatg atcagcggcg tcaggatgag ctgcatcgcc acgtcgagct tgccgccggg
      961 gatgacgatg gagttcgccc ggctcatgaa gctgtcgtgg atcatcgaca gcaggtacgg
     1021 gaagtcgatg ccgcgcggat tcttgaagcg gatcaccacc atcgattcgt cggcggtggg
     1081 gatccagcgg gcgatgaacg ggttggacgt gtcgaccatc ggcacgcgct ggaagttgat
     1141 gtcggtctcg ccgaactgcg ggcagatgaa gttgatgtag tccggcatgc gccggaggat
     1201 cgtgtcggtg acggcttcgg tggagtagcc gcgcgccgac cgatcgcgat gcagcttctg
     1261 gatccactcc aggttgatca ccggcaccac gccgatcttc aggtcgacat gctgcgccag
     1321 gttgaccttg tcggtgacga ccgcgccgtg cagtccctcg tagaacagaa ggtccgtgtc
     1381 cgcggggaac ggacgccagg gcgtgaagtt gccgggctga acggcgaatt ccgccgcttc
     1441 ctcggcgtcg tggacatagg tgcgcgtttc gccggtaccg ttctcgccgt actgtcggaa
     1501 cgtgtcctgg agcttttcca gaaggttggc gtagtcgccg aagtggctgt aattgttgtt
     1561 gccgtcgatc gccgcctgcg acatgcggtg gcgcatctcg gcacggttgt aacggtggaa
     1621 ggcgtccccc tcgatgaagg cagcgctgac gccttcacgc cggaaaatct cgacgaatgt
     1681 cttcttgacc gaggtcgtgc cggcgccgga agagccggtg atggcgatga taggatgctt
     1741 gatcgacatg accgtctcct ctagccgcgc agcaggccgc gcttgccgaa caggggcgag
     1801 gtttcgacgg cggcgctggc ttcaacgtga taacggcgaa tgcgcgtcac cttgtcgctc
     1861 gaaccgaaga tcaagggcac gcgggcgtgc agactggcag gcaccagatc gagaatgcgg
     1921 ttgaccccat tcgtcgcctg gccgccggcc tgctcgacca gcagcgcgat cgggctcgcc
     1981 tcgtagacgt gccgcaggcg accggattcg tagcctttgc gcctgtcggc cgggtagagg
     2041 aacacgccgc cccgcatgaa gatgcgatag gcgtcggcga ccagcgaggc gatccagcgc
     2101 atgttgaagt tcttgccgcg cggaccgtcg gcgccctcga tgcagtcatc gacgtaggcc
     2161 tgcaccggcc tgtgccagtg gcggtagttc gaggcgttga tggcgaattc cggcgagcct
     2221 tccgggatga tcagcgacag gcgcgtcatg acataggagc cactgagcgg atcgagcgtt
     2281 gccatgtgca cgccgacgcc ggttgtgaag accagtgagg tctgtggtcc gtagatgatg
     2341 aagcccgctg ccagctgccg tacgccgggt tgcaggaagt cggcagtcgt cggtgccctg
     2401 tccgggggcg cggcgagcaa agagaagatc gtgccgatgg aaacgttggt gtcgatgttg
     2461 gacgagccgt ccagcgggtc gatggcaaca aggatcgtgc cgtcggtatc gacttccagc
     2521 ggctcgtcca gttcttcgga agccatggcg cgcacgcccg agccctttag gccctgcgag
     2581 aattggcggt cggccagcac gtcgagcgcc ttctgggcgt cgccatccgc attgccggtg
     2641 ccggtcgccg caccgagcgg gccggccagc ggcccgccgc ggatgaccga gacgatcggc
     2701 accgatgcct gcgcgatggc cgcgaccacc gatgccaccg gcgccggggc catcgccgac
     2761 agtgcgtcct ccagtttggt gccgatctgg actttgtcca ctgtgccgtc ctcgcgttcg
     2821 aacccatgtg acgggtgcat tccgcgtggc aaatatgagg acttgctaaa aaattaaaac
     2881 tgactaattt ttcaacttca ttcagaaaac ttgaatccca tgtcccagct ttcgctccgc
     2941 catttcgaga cgctccaggc cattgtccgg cagggcacgc tggttggcgc ggcatcctac
     3001 ctgaacgtca cgccggccgc cctgacggcc cgcctgaaag cgcttgaaga ccttgtgggt
     3061 cttaagctgt tcgaccgcac gtcgggcggc atgaagctga ccatggccgg agagatggcg
     3121 ctcgactccg cccatcggat cgagcgcgag gcgaaggatt tcttggcacg gatggccacg
     3181 atccggcgcg gccagggcgg tcgcctggcg gtcgccgcag tttcgacagc caagtacttt
     3241 gcgccgcgat tgatcgccgc gttcacgcgg gaacacccca acatcgacct gcgattcctg
     3301 atcggcaatc gcgacgacac catcgcggcg ttgcagtcgc tcagcgccga agtcgctctg
     3361 accggacgcc ctccggcgga catgccagca gagaaggcag cgcttggcgc ccatccctac
     3421 gtcatcgtgg cgccaccctt tcatccgctg gtgggcgagc gtgcgatttc caaggatcgc
     3481 ctggggcagg agaccttcct gtttcgcgag cagggttccg gcacgcgctc gatcttcgac
     3541 tacttcattg gcgacatcac cctgcaccgt gcgcaggtcg gcatcgaact ggggtcgaac
     3601 gaaaccatca agcaggctgt gatggcaggg ctgggtgtag cgctgatttc cgcccacacc
     3661 attgccgcgg aggtacagga cggtcgcctg cgctgtctcg acgtcgaagg gctgccgatc
     3721 attcgtcagt ggtttgtcgt caatcgggcg gaccgcgaga tctcccccat cgcacaggct
     3781 ttccgggcct tcgtgacgga gcgcggcgcg cattttcttc cccgtttgat cggggggcac
     3841 cgcgacgagc gcccgctcac ggtcctgcca ccggaagaat aggcgtcttg cctcgactgc
     3901 gaacaatccc gaagggataa gtgttgcgca agagggattc gtgaaattat aataagacgt
     3961 tgaaatatca aattttattt catgtcgtat tgataagacg ccaacgaaag cgccaacatt
     4021 tttcatgctt gctcttggct ggcctcactc catcatgttc ctaaaacagc gctaactggt
     4081 ccggctttgt cggcctggcg gcatccgttg ttcctcggaa gctcgtcatc gccgcgtatt
     4141 gctgatcggt tagcgataga acgtcgacct tgccttcatt gggcaccttc ttgccgatcc
     4201 gtcgtgtcag gccctcgatc tgttccttgc ccgaggtgaa tcggaggtag accgagaact
     4261 gcatcatcga gaatccctcg tcgagcaggt actgccgaaa tcgggtcgcc cgcttgcgct
     4321 cggtcttggt caggacgggt agatcaaaca ccacgaaaag ccacatgatg cggtaacctg
     4381 ataaggtgga gttcgcctgt gccccggttg cgctaggtgc tgcagccatc gttcgcctgc
     4441 agcgggatga ggggatcggg aagcgccagt gaagccgacg gatcggcgaa ggcttggctt
     4501 aacgattgcg ccagcctgac gagggcattg ctcaggggcc ctgtgccagc gggagaacgg
     4561 aagtccgaag ccagtacggt tgcgagcgct cgcttgacgc ccggcgtcag ttcgaggcga
     4621 ccgtcctcag ccagaccaag caccgtcaga tcgacggtcg ggcggaaagg ctcgatcaaa
     4681 tcgtcagcca agcgaagtcc gtcgcctcgc gacccatgaa acaggctgag cgacgggttc
     4741 atgcccgccg ctacgatggc gcgcgccgtt gctgcgcgaa gaacggtgta accatagttg
     4801 aggagtgcgt tgatgccttc ggagttgcga tcccgccgaa aaccgtcgcc aaacatcagg
     4861 gggaaatatc gctgcgcggc ctgcgcttcg agcgtcgcgg cgtcttccga ccggaacgcg
     4921 cggatcaggc ggcgcaattg tgctgacggc cgctccgtgc gctccagcgc ttcggcctgt
     4981 gcagtcagct tggatttgac aatctgcgcc cagagcctgg cctttcttgc tctgggacaa
     5041 cttgcctgcg cttccatgcg cacgccctga gcgtggtgac cggagaccgg cagcaaccat
     5101 gcgaccggcc tgaaatcttt cccgcagatc gctagaggcg cgccgcgttc ggcgagggcg
     5161 gccacagcct gattggtgta ggaaagcgaa ggcgtcgctg caattacggc ctcgacatca
     5221 tccagcggga cctctcccag aagcccgtcc ggccccgaaa tgctgaggaa cccgcgattg
     5281 agcgacagcc gccgcccctc gccgctgatc tcgataacgc gtctgagcat ggctgctctc
     5341 cccgttttgg agcagcatac ggcgtcattg ggtcctaaga tgagcgaaac cacttattaa
     5401 cgtgaagcgt tagggttagg accgcgcctt cgcccctgct agaacactac gagttcggcg
     5461 acgactggcc ggatggttgc ggttggaccg cgccttcgcc cctgctagaa ccacgccaag
     5521 cgggcgccgg tggatgatca ggttgcggtt ggaccgcgcc ttcgcccctg ctagaacgtc
     5581 ggccagtgcg cggtcaccca gccatccgtt gcggttggac cgcgccttcg cccctgctag
     5641 aacccattgt catccttgtc ctcgatcctg tccgttgcgg ttggaccgcg ccttcgcccc
     5701 tgctagaaca gcgcaccatc agggcacgcg gccaaaaggg ttgcggttgg accgcgcctt
     5761 cgcccctgct agaactccac atgagcaacg tatccgaaac agccagttgc ggttggaccg
     5821 cgccttcgcc cctgctagaa ccgcatcatc gtgccgccgg aggtgatcaa ggttgcggtt
     5881 ggaccgcgcc ttcgcccctg ctagaacggt cgtcgcgttt tcgtgggttg aggaccagtt
     5941 gcggttggac cgcgccttcg cccctgctag aacacgcagc cgaggttgcc cagcgccacg
     6001 caagttgcgg ttggaccgcg ccttcgcccc tgctagaacg gcagcttccg acgcgctatc
     6061 ggctggcctg ttgcggttgg accgcgcctt cgcccctgct agaaccgctg ggcttcacgc
     6121 ctcaccttga tcgtggttgc ggttggaccg cgccttcgcc cctgctagaa ctgacgggct
     6181 gagttctgcc cagatcgcgg agttgcggtt ggaccgcgcc ttcgcccctg ctagaacgcg
     6241 ggagcagcgc cgggatgctc cgcgccggtt gcggttggac cgcgccttcg cccctgctag
     6301 aacggctacg acgcgcgggt gatgcgcgag gtggttgcgg ttggaccgcg ccttcgcccc
     6361 tgctagaact aggcgtccgt cggcgcaccc atggtggcgg ttgcggttgg accgcgcctt
     6421 cgcccctgct agaaccggga aacggatatc gtccgcgaca atcaagttgc ggttggaccg
     6481 cgccttcgcc cctgctagaa ctgcactgat cgcggatcga gcgcgcccag tgttgcggtt
     6541 ggaccgcgcc ttcgcccctg ctagaaccgg cgcgcatgct ggcacgatat tcgggacgtt
     6601 gcggttggac cgcgccttcg cccctgctag aaccgaatca cctttgcgca ggctggcgcg
     6661 caggttgcgg ttggaccgcg ccttcgcccc tgctagaact cctcgtgaaa aagatcgccc
     6721 atggaattgg ttgcggttgg accgcgcctt cgcccctgct agaaccgagg cggatatccg
     6781 cgatctgatg ctcgcgttgc ggttggaccg cgccttcgcc cctgctagaa ccacgagagc
     6841 catccggccc gccccgcgca ggttgcggtt ggaccgcgcc ttcgcccctg ctagaaccac
     6901 cgcgtacagt tccagaagcg cggggctgtt gcggttggac cgcgccttcg cccctgctag
     6961 aaccgacttc ccataagccc ctgttttcag gggctttttt tatcttttcg cagggagccc
     7021 gccaccattg gtggcgggcc cgtggttcta gcatggtggc agggtagtcc agccacaaca
     7081 cacacaaccg tctcgtacaa acgttgtgtg ttgatgacgc aactttgacg ttgcgggttc
     7141 aagcatactg gatcagaatc cagatcactg cttgccccgc aaatggtggc attcggttgc
     7201 ccttagcgat gggggcagtc tgcccggagc tcccttgagc tgtctgacgt gctccccgtt
     7261 ttccggccgg tcataagtta ggtccggctg cgttctggtc cattcagtca gcgacgcagc
     7321 ccagcctcgt ccacgcacac aatctcggca ttgcgcacac taagtccgga aattgcagcg
     7381 aggtcccatc taaatggatc ttctggatcg tcgtggcgtt tctggaagtc accagcttcg
     7441 caatgctgaa caagatatag ccgttcggca gcggcattca gcctcaccac ccgcttgacc
     7501 cgccgcacgc cgtccttgtc atcgatctcg agaaggtcgc ccttgtgcag gcgcatgacg
     7561 agcttgccgc ccagcttttc gcgctcccac aagggtcgcc agtcccgctg gttcacctcg
     7621 aacagggacg cggcgaaggc cttccagatc tgtccgccct tgccgtccct gaaagagacg
     7681 acgtcgagac accaattctc gtccggcacg acggccttgt agggcctgcc cgtccgccgg
     7741 tcgcggatcg gcacggcatt ggcctttggc ttgagcagcc ggacatggcg cacggggttg
     7801 cgcagcttgc cggtgcgcgc cgcaatccgt tctgcgtccg ccttggccca ggcgagcagg
     7861 gcagccgaca gcgccttcgg gtccggcttt ccgccttcgc ccgtcacttc gtgcaccacc
     7921 tgttgcagcg ccgcgcgcag atcagggtcg cgtacccttt cgatctcgtt gggattgagg
     7981 ctgtccagag gctttcgatg caccagattg ccgatggcct tgtcgcgctc gcttttcacg
     8041 atgccatagg cggtgtcctc gtgcagcgca ccgcccttgc cgtgctccgg cttgtggctg
     8101 acgatcatcg tgcggacgag atcgcgcaag cgatcgcgga agccgggaaa aggctcgccg
     8161 aatccggcga caacgcgcgc ggcgccggtt tcctcgttgc gggccgcctc acgcgccacc
     8221 cgttgcagca tcgagcgcga gatgcagccg atggtggcgg cgtcgatcgc gtggtggcga
     8281 tgatcgttgc ggttcttgcg gttgtcatcg cccaaaatgc tgtcgagccc ccattggcgg
     8341 cgcatcagcg cggtaagctg gccggtgacc acccagacgt cgttctcgtc gccgcagacc
     8401 ttggacagat aggctttggc tgctctggcc aggtgccgcg tctcgttgag ctggcgggcc
     8461 agcatgtcgc gctcgcggtt gtcgaagcgc tccatcgcat cctcgcggaa gcgccagcgc
     8521 ttgtacggcg ggagcgtctc ggcgtttgcc gcgatggccg gccaatccgg cgtcgcgcca
     8581 taggcttcga acggggtctg attgcgcttc ttgcggttgg cttcgcggaa gcacaggacc
     8641 ttgttggcca tgccgtcgtc gagcgtgcgc gagaacggca ggacgtgttc gatctccacc
     8701 gcctcggtga aaagttcatg cacgccgatc acccgtagcg tgtagggaca gcgcacgacg
     8761 ccgccggcca tctgctcgtc gtagaggcgc aacagagcca tgttcagacc gttttcgggc
     8821 aggccgagtt ccccgagctt cttgcggcgg atgtcgttct gcttgcggtt ctgggtgttg
     8881 cggcgctggt cctcgtcgcg ctggcgggaa ctgcgcttca ggtcgcgggc cagttcgatc
     8941 acgatctcgt cgggatggcc aaatcgctgg atcaggcggt tggtcacgcg cctgagctgg
     9001 ttcaggccga tatgcaccgt cgggtttgcg atgcggccaa aacgtttggc ttcgtccttc
     9061 tcgtcgggat cggcggaacc aaagccgaca tgccgctcga tgacgcgggc gtaatagggc
     9121 aggcgcggga aaatttcgcc gtcgcgaaag tcggaatggt gctggaacaa tcccgacttc
     9181 tcgccagccc ggcgaacggc ttcatcgtag gtgatgacgt ctgccgcaag ctcctgcacg
     9241 atcagggcgt tcgcggtcgc gccaagacgg ccatagcctt ccggcagcgc gacgccgctg
     9301 acgtaagctg catgcgtctc atccaaccct gtttccgcca tcacccaggc aaccgctgca
     9361 tccgagtcat cctcctcaag aagcacgcgg acgatctgct cctgttttga cacctgcatc
     9421 tgctgccagg ccttgccgaa cccgctgtcg cgcgacaggg ccttggccgt cttcgacccc
     9481 tcgatcctgt cgcgcccacc ttcttccagc gaaaagcgaa cggtcgatcc aagtttgagc
     9541 gtcttgcgga tcagagtgaa ggacaagtcc ttacctgcca tcagcgtctg tgcgaggacc
     9601 tttcgctggg caaggtcgag ggattgcgtc aggttgaggc cctcgccgaa gcgcagcgca
     9661 ttcagcgttt cgaaaacgac acgcgcctgc acggaaggca gcgccatagg cagccgttct
     9721 tcttccggaa ccaacgtgca gcgccccggt ttgaccggcc tgagaggccg ctggtcgaag
     9781 attgccgcac ggatcgcggt tcgcgcgctt tcggtcagca catgcggaag gtgtatggcc
     9841 tgtgccgccc acagggcgtc gaattcatcc tcgacgattt tccgggtcgg atagaggtcg
     9901 tatgtgacct tgcccggcgt agcgcctgca gcctgccgaa agcgtgaggg cgatccttcg
     9961 gactgccgct tgtatagaaa ctcgccgagc gtccgcgcgc cgttcgtctg aatagcctcg
    10021 cgcaggcgct gtgcgccttc agccatcgct ccggacaccg cattcttgcc gtcggtgcgc
    10081 cggttggact tgaatccccg gcgctgattg aggtgaaaca gcgcccggcc aaagtgaatg
    10141 agcggcaagg gcgcatcgag gccggttgtg cgcagctgaa gggggtccaa gcgctccaaa
    10201 gccttttgct cggctttgtc gaccgggagg aggccatgtg cttctaacaa cgcgatcaac
    10261 ttgcgctgcc gttgcaggaa tcggtctcga cgtttgcgca tacctctggg aacccgccgc
    10321 atgacggcga gcgactggcc gtccttcgga ttgcgaccgt cagaaaagac gcgcgtgccg
    10381 actgcagcga tttcggacgg ctctagccgc cgttctcctt gagcggcttc caatgctagg
    10441 tcccggaaca ccgcccatcc aagggaattc gtgccgaggt caaaagagaa acgcagtcca
    10501 gaacccatgg ccgcctcctg ccgtcgcccg ctgcggtgcg cggggaattc tttttctgag
    10561 gcgatctttg cagcgcgtgt ttccgattgc aatgccggaa gtctggtcta tactgtcgca
    10621 gttctagcaa gggcgcaggc gtggtccctc cgttaacaag gggatcaccc caccagatca
    10681 gggcgggctc cggtccgccc tttttcgttg gttgtggcga aacgtgtgtg cggagtggtt
    10741 tggccagtca aagcgagggt gcctgtttgc gttgagcgcc gcgagacaga tagccgcccc
    10801 gccagcgccc ggttgagtgc agttccgacc gcacgagttg gtttgcatgc gtcctgtgca
    10861 tatgatgaat tgtctcatgc ttttttcgca ttcctcgcgc gtgcgcgcgc gttgggtgga
    10921 cgtgcgtgtg cacgatcgcc cgaagaaaga tgaaaaacgt cgaccaagtc atcacggctg
    10981 aaagtgaccg cagtgacagc gtccatcgcg cgccatgcga gacgcggcgt ccgatggcgc
    11041 ggcgctcaac tccggtcgtc atctcctgtc cgggagggca gcagcttagc gacgctggat
    11101 acctttgcgt cgatggttac aggccgctcc tcgtggggat caagcttcag cgtccatccc
    11161 gggctatcgt ggtcttcgat cagccagacg gtgctgccat cggcaaggcg gccgcgaaag
    11221 tagcgtctgc cgctcttgtg gctaacgccg ggctttatgc tggcgagcct gatcattgcg
    11281 ggcgatccct ggcggcgctg ggcgtgaagc cctgcgttgg atcgatacgg cagcacttca
    11341 cggtccgggg cgcgaaggcc tcacgcgatt ccgcgagcat gtagtcccag ccgtcgccgg
    11401 tgaggccatg gaaccaccgg gcgaagggcc tgcctggcgg ctcatggcat gtcatgcggc
    11461 acaggacctc gaggccgtcg ttttcgttcc cgctcatgcc gcgatctcca gagccggacg
    11521 cgctggaagc gcgtcggctg gcggggcagg cgtggtgacg gtgattttgg cgagagcatc
    11581 cggtgagacg gatacgcgcc gggcgttcct gtgtttctta tctacatatg tcgtgcgcga
    11641 aacagtctca cgcgcggctg cgtcgcgagc ggccttttcg cggtaccagg tcgctcgtga
    11701 aactccctgt tcgcgccacg gcttcgcctt cgcggcgctc tgcgccctgt ccttggcccc
    11761 tgccttccgt cgccgcgcct tgtcggcttc gcgcttgcgg agctttctgg actcagcccg
    11821 ctcggccggc gaaagatcga acggttcgat ggtccatgcc ctgagccgct gacggtcgat
    11881 ttccgacagc cggaggatgc gcccagccgc cgcgccgtcc cacggcgccg gttcagcgct
    11941 gacgatgtcg atcagtgccg cgacattggc cgggttgaag gtcggggcgc gctcccgcac
    12001 ccaaccccgg aagttgtcgg gccagccatc catggcgtgg cagagatgcg ccgtgacctc
    12061 gataacggcc tgcacgcaat cgcgatccgc gcggccgcca tagcgaaggt cgagaacgcg
    12121 gttcacctgc ctgagccgga tcggcgcgag gtcgcgcggc ttccgcttgc ccgatgtggt
    12181 gaagcccttg cgcactcgtt tgggcgcttc cggcggggct tcgatggcga tcagcgccgc
    12241 gcgaaattcc ggcgcgctcc tagacgcgcc ggcttgtctg gtatgtctca tggataccta
    12301 caaacggcct gcttgccaga gttctgaacg ccctcggacg gcaatccggg ggcgtttgct
    12361 attgtgtgat ggtgattgtc ggcatggttt caggccgcgc ggggctgatc gcttgctgcc
    12421 tgctcgcgtc gccccacgac cgggagcgag gcgcggaagc gcaggatttc cgcctccgcg
    12481 atcagggtgc gccgcccgtt cttgatggtg gcaatctcgc ccgcgcccac gagggcataa
    12541 aagcgcgtca agcgcatgcc catcaggggc agcgcttcct gcacggtgta gagtttgtcc
    12601 ggtgtctgca tgcttctctc cgttcgccgg ttcgtggcga atgcgggaga agggtgatgg
    12661 aaactggtga ctcgatgcaa acgcctaaat gggcggcttc cagtcgattt gaccgaactc
    12721 attgataatg cgtcgcattc tgtcgttgag tcttcggccg gagaggtctg atagtgcgag
    12781 tttgaccttt tgggtgacct caggcggcaa ttgtcggcgc tcggcgaggg caacaaaaac
    12841 gcgctcgcta gtgtcgatag ctgactgaat gaaacgggcg aattcgttgc cttcctcgga
    12901 tgtggttgaa ggcagtttgg ttgcaacacc tgcggctttc gcagcggcta gcaggacaag
    12961 caaaaagtca tcgacgatct cgtagacgcg gtgtcgaatt ccctttttct cacccttgcg
    13021 gcgctttgct cttgctgcga tctcggcagc ggcagcacgg tgtcttgcgt gaattttggc
    13081 aatttcgaac gggccatgag gaaaagtgtc gtcgtcttcg cactttgctt cgatatcgat
    13141 atagaacgct agcgctattt cgcaccgccg gcctgctagg ctctccggat cgattggttt
    13201 tccattgatg aaggcagtgg ccaaaagcct taactcatcc cgttcaacga tggtggattc
    13261 gatcagtgca tgacaaaagt gaatggcctc gagcggaatg tcgatgctgc cctgttcgcg
    13321 cgccatattc caatggtgaa acccaacaac ctctgggtcc ggcttccttc cgaacgctct
    13381 tgaagtttga attgcttgga aaaaagtctg gcacgccaaa ggcgagttct tccactcgcc
    13441 gctcatctgc cacacactcc ggcgctcgcc atgccgtgcg ccttccccat ttgcgctagc
    13501 tgctcggtgc gatgcctgcg atgctctggt gcagccatcc tgcgggttcc tttgctcgcg
    13561 gtgaaagaat ggcgcggcag caatctgcgc cttcgcccgg accgcatgcg ttcagcccgc
    13621 ctgaaacttc aaaaccttgc cgccctggcg catccggtcg ttttcgtcgg cccaccattg
    13681 cgcaagcgcc acgcgctcct gccagtaggc gccccgcgca tagatcgcgc ggatgccgtc
    13741 ctgatccgca tgggcgaggg cgcgctcgat ggcgtcggga ttccacttgc cgctttcgtt
    13801 cgccatggtc gagaatgacg cccggaaccc gtgcgccgtc atgtcgtccg ggccgaaacc
    13861 cattcggcgc agcgccgcat tcagcgtatt ctcgctgatc ggcttgggtt cgagcacgcc
    13921 gccgtccttt gccgtcttgc cgtagcccgg aaacaccagc cgcccgcgtc cggtgagcgc
    13981 gtgcagggcg gcgaggatcg caacggcctg ccggggtagc ggcaccttgt gctcgcgtcg
    14041 catcttcatg cgctttgccg gtacggtcca gacggccttc tcaagatcga actcgctcca
    14101 ttccgccagc cggagttcgc cggggcgcgc tgccagcagc gccagcagct tcagcgcggc
    14161 aacagtagtc ggctggccct gaaagccgtc caccgcccta agcagcccgc caaatgccat
    14221 cggttcggtg atcgcagcgc gatgcttcac cactggcgcg gtgagtgctc cgcgcagagc
    14281 ctgggtcggg tcactcgacg cgcgcccggt ggcaatgcca tagcggaaaa cctcgccaat
    14341 catggatcgc atccgtttgg ccgtctccag gcggcctttc gcctcgactc ttcgcaagac
    14401 ggccagaact tccggcgcgg caatgtcaga caggggccgt ttgccgaagg cttcgcctgt
    14461 aagtccgaaa atccattcgt tcttggcaag cgtcatctcg gctcggcctt cgcagcgctt
    14521 cttcgcaagc agttcagcgg cgacgacggc gaaggtggcc tgttcggtgt caatcctgct
    14581 gatcttgtcc agtcgtcgct gcaggcccgg atcaatgccc aggaccagca gctttcttgc
    14641 ttcctccgcg cgctggcgcg ccgcctgaag cgacaagtcc ggatacagac caaacgacag
    14701 ggcgttctgc ttcccgccga acctgtaggc ccaacgccag aattttccgc ctgtcggtga
    14761 gatgtggagc tggagcccgc gcccgtcgct gatcttgcgc agcttcgcgc cgggtttggc
    14821 atttcgaatc gcggtgtcgg tcaacgccat cgaatccccc ttcgcgggtc cgaaatgttg
    14881 gcggatcaga acgccaacac agacgccaac aattcgtgcg gctggacgcg aacacaggcg
    14941 aacgcccacg aaggcaatct cgctcaaaaa cgctgacttt tcaaggaaat tcgaaggctg
    15001 gcgggccaac acgaatgggg aagtggtgcc gcaagaggga atcgaacccc cgaccccatc
    15061 attacgaatg acgtgctcta ccagctgagc tattgcggca tcggcgtgca catagcgggt
    15121 gtttgcgccg agcgcaacca tccctgcaag gccgctgcca gcgctttcgc ccttgccgcg
    15181 aacgctggtt gcgacgtgtt cgcgatggtg ctcagccgaa cagccgccag cctcgcttcg
    15241 tttcgtcagg ccgacgcggc ccggggtcat ccggcgcatg ttcaaggggt gacacgggct
    15301 gcgcgcccgg cgccgccgga ggtgtttcgg gctcgggcgg gagcacgacg gccaccggct
    15361 ccgaaacagg cttcggggcc tccatttcgg ctgtcgccag ggacggggca gcttcgatcg
    15421 ccggggcagc ggcgatcgtc tcgggctccg gttgcgagaa atggccggcg tcaatggagg
    15481 cgcgcaccga ggcttccagt gactgcggtg gcgtcatcca gacaaaggcg tccagttttc
    15541 cggtgaccgg cgagacggga gcccaggcat cggccaccag tccgtcggcg acccaggccg
    15601 gatcgcgcgg cgcgatcgac gcgcgcgaca accacttgcg caccaggccg gcggcattgt
    15661 gttccttgtc ctccagctcg gccatgagca cgcaggcgcg cgcggtcggc cgctctagca
    15721 ccagaggctc gagcaggccg cgggcttccg cgaattcgcg tgcgtcgatg gcggcgcgga
    15781 cgagggtgag ctgcgcctcg cggtccttcg gcgtcagctt gacgagctgg cgggcacgct
    15841 tgagccggtc atggctggaa tcgccgccgc gcacggacag ataggcgtcc gcaagctcag
    15901 gatgggtgtt gacgcgccag gccgcctcga tcacccgtgt ggccttgccg tagtcacccc
    15961 gcgccgaaag gcgccgcgcg gccagggctg ctgccggcac cagcccggcg tcgagcttca
    16021 gcgctctcag cgcgacgtcc agtgcctcgt ccggcgcacg ttccgccaga tgcagcgcct
    16081 cggccgtcag cagcacagcc ttctgccgtt tcgcgacgtc ccgatcgacg acgcgccggc
    16141 tggcattctg gtcgacggcg gcgacggccc cgccccagtc cttgtcggcg cagcgatact
    16201 ccagcacggc ttcgctcgcc cagggcgcgg aaggcgcgat gcgaaaggcc tcttcggcga
    16261 aggatcgcgc ggcgttgttg ccgttgcgcc gggcctcgac gaacagcccc cgcaagccca
    16321 gaacgcgcgt ctctgggtct tcgagcatgc ggttgaaggt ggcttccgcg ccctgctggt
    16381 cgcccgccaa ctgggccgac tgcgccttga gcagcagggt caggggctcg ttgccgagca
    16441 gccgttccgc ctcgctggcg aatttcgcgg cggcgcgccg gtcgccggcg ccgaccgaga
    16501 ccatgccgcg cgagacggcg gcatagccct tcgccctgcg gcgcacctgg gtggcgacgc
    16561 tcaggatgcc gggcaggcga aacagcaggc gcagcagcgt ccagaccagc atcagggcaa
    16621 cagtcatcag gaccagcgcg cctgccgcct tgagcagtgt cacttggtac tcgaatcccc
    16681 cgtagttgag gacaacatgg ccgggcgttt ccgcaagctg gatcacgccg aacgcggcgg
    16741 ccagaaccag tgcgaagaac aggaacatgc ggatcatggc cggaaagcct cctcagttgc
    16801 ggcgggcggc ggcgtcgagc gccgcggcct cgatgcgccc gatcgc
//
LOCUS       DAOMXN010000032        15545 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_2113_length_15545_cov_10.990316, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000032 DAOMXN010000000
VERSION     DAOMXN010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 15545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15545
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2113_length_15545_cov_10.990316"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..1062
                     /locus_tag="PK812_04565"
     CDS             4..1062
                     /locus_tag="PK812_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRJ68861.1"
                     /translation="MIEANQASEHFFDLSRALLARRSLSDLVPAGSPLLALIEQVRQR
                     NAGITEYAVDLVTQRAIAERLTGERLLDLYASPVANRAGHVIVVLQERSMADKMTRQL
                     THRGAARSVSAMSAMLAHEIKNPLSGIRGAAQLLEIDADDTARELTGLIRAEVDRIVR
                     LVDRFEVFADGAPAPFAPINIHEVLDHVIRLAASGFARDIRVTKDYDPSLPPVFGNGD
                     QLVQVMLNLIKNAAEAIHDFREDGEITVSTAYRPGVRVLVPSSGERISLPLEVCVVDN
                     GPGIRESLIEHLFEPFVTTKASGTGLGLATVAKIVSDHGGVVECERVKRGTMFRILLP
                     LHRPAAVRREAGSRTDAE"
     gene            1052..2554
                     /gene="ntrC"
                     /locus_tag="PK812_04570"
     CDS             1052..2554
                     /gene="ntrC"
                     /locus_tag="PK812_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HRJ68862.1"
                     /translation="MQNSAKTILIADDDAAIRTVVMQALTRAGYSVRSTGTAGVLWRW
                     VSAGDGDLVITDVVMPDENIFDVLPRIRRARPDLPVIVMSAQNTFMTAIKASERGAYD
                     YLPKPFDLNELLSLVRRALAAAAPAKPAQPAQQNDEIPLVGRSPAMQEIYRVLARLMQ
                     TDLTVIITGESGTGKELVARALHDFGKRRRGPFVAVNMAAIPKDLIESELFGHEKGAF
                     TGANTRSAGRFEQAEGGTLFLDEIGDMPMEAQTRLLRVLQQGEYTTVGGRTPIRTDVR
                     IVAATNKDLTVLIEQGLFREDLYYRLNVVPIRLPALRERAADIPDLARHFLMLAEKGG
                     LAHKTFNGSALQQLQHYPWPGNVRELENLVRRLVALYPDEVITAEMVENELGEARRLT
                     PSAGPSAHPQPPAAQARGAAAFQGGSTDTIESFLERHLVDYFASFGSDLPPPGLYDRV
                     LREVERPLVAAALGATGGNQVKAADLLGVNRNTLRKKIKDLGIRLVSTIR"
     gene            2697..4931
                     /locus_tag="PK812_04575"
     CDS             2697..4931
                     /locus_tag="PK812_04575"
                     /inference="COORDINATES: protein motif:HMM:NF039586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HRJ68863.1"
                     /translation="MNSVTEIPQLDPPVGRARRLLGYAEIGLFGAALVAAAATIAILM
                     GVGVRPSETAFIVALAADILLALLLVALVGVRLWALNRARKHGQAGARLHLRIVTLLG
                     LVAGLPTALVALFAMITLERGLNPWFSGDLKILVENSDKIARQFQQQLCQNVVREMRL
                     MAVDVERAVTSGIYQSDRKLFREYMTSRAVFLSLPFSAIIKPDGQVLERAELGTSLGE
                     FTPNAEDFAQAATEEPPCIFSQQTLGTLTKLRGFDDGYLIVGRPIDRRAIEFPLIAQA
                     GVQQYQTLDSRRAATQLGVGVVFVLITLIVLLAASLLGLRFADRLVQPVRRLIMATDA
                     VSSGNLYVQVPVGTEDSDIAHLGSTFNNMTSVLRSQHNSLLAANQLNDQRRRFMEAVL
                     SGVPAGVVGIDGLGRISIANPTAERLLGAEPGALTGRELVDVLPQIKSVLDEARIGAH
                     RVLQETISLSQAGRDRTLSVRIAHEASGDDEMGLVVTLDDITDLVTAQRTSAWADVAR
                     RIAHEIKNPLTPIQLSAERIRRKFGKVIAEDKAIFDQCTDTIVRQVEDIKRMVDEFSS
                     FARMPKPRPESEDIVAVIKETAFMMRVAHPDIVIEEHLPAKSVRATFDRRLIAQAMQN
                     IIKNAAEAIAAVPNGERGPGRIELRLFHPEPDRVAIDVIDNGKGFPSENRDRLLEPYM
                     TTRESGTGLGLAIVAKILEEHGGGIELRDAPSGRGACVRLLLPSAAVATPAAHPAQPT
                     DRHA"
     gene            4928..6292
                     /locus_tag="PK812_04580"
     CDS             4928..6292
                     /locus_tag="PK812_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003600268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRJ68864.1"
                     /translation="MSQEILIVDDESDIRELVAGILEDEGYATRSARNADEAIAAIES
                     RRPHLLFLDIWLQGSRLDGLQLLEVIKQSHPELPVVMISGHGNIETAVSAIKSGAYDF
                     IEKPFKADRLVLVASRALEAQQMKREIQSLKTRSGQTDRLVGRSAAMNQLRQTIDRIA
                     PTNARILISGAPGAGKEAVARAIHGASLRASGPFVVTNAATITPERMEEALFGVEGGP
                     NQKRRNGALEDAHGGTLFIDEIADMPKETQSKILRVLVDQNFQRVGGATRVFVDVRII
                     SSTSRDLKAEITAGHFREDLFHRLAVVPLLVPGLSERREDIPELIDVFMDQISMATGL
                     PKRLIGPDALAILQTHDWPGNVRQLRNNIERLMILAAGDPSTVVTAEMLPQDVGSAAP
                     PTPAGAGGERLLGLPLREAREIFEREYLIAQVNRFGGNISRTAEFVGMERSALHRKLK
                     SLGV"
     gene            6493..6738
                     /gene="hfq"
                     /locus_tag="PK812_04585"
     CDS             6493..6738
                     /gene="hfq"
                     /locus_tag="PK812_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HRJ68865.1"
                     /translation="MANERGQNLQDVFLNHVRKNKIPLTIFLVNGVKLQGVVTWFDNF
                     CVLLRRDGVSQLVYKHAISTIMPGAPVTLSSPMEDEA"
     gene            6769..8154
                     /gene="hflX"
                     /locus_tag="PK812_04590"
     CDS             6769..8154
                     /gene="hflX"
                     /locus_tag="PK812_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003600263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HRJ68866.1"
                     /translation="MKDRRTPDLPSFDAASAAEQRVAAHTRTLVVGPYLQERAAARAG
                     ETATRGFEARLDEAVGLAGAIDLNIVRAEIAPLKTPRPATYLGVGKIEQFKALVEQED
                     IALVLADCALSPVQQRNLEKALGAKVIDRTGLILEIFGRRARTREGALQVELAHLHYQ
                     KSRLVRSWTHLERQRGGFGFLGGPGETQIEADRRLIQERMKRIERELEDVSRTRGLHR
                     ASRDKVPFPTVALVGYTNAGKSTLFNRMTRAEVLAKDMLFATLDPTTRVIELPHGKKA
                     ILSDTVGFISDLPTMLVAAFRATLEDVILADLILHVRDVAHEDAEAQGADVRRILGEL
                     GIDEAQSARVLEVWNKADLLPPDERERLLGLSARERPGEGRPVLVSALTGEGIAQLQA
                     VIEERLSQGHERRQVLMPGSDGGGIAWIYENCEVLGREDRDDGTVALDIRLSPERVER
                     FERRIRPLSGA"
     gene            complement(8371..8580)
                     /locus_tag="PK812_04595"
     CDS             complement(8371..8580)
                     /locus_tag="PK812_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68867.1"
                     /translation="MSAASNCRPEAPGSALPRLPDNLDAIRSSCEALNAMRSDIETDM
                     DAASRERLLGLSAEIRKLAAMIRRP"
     gene            complement(8721..9098)
                     /locus_tag="PK812_04600"
     CDS             complement(8721..9098)
                     /locus_tag="PK812_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68868.1"
                     /translation="MSASKLQRITLHLARTKDFPNGSYKHGYEFVAPLDADAHIDAEA
                     WHKVREQCRVKRFWANDEDDLGHLTRRPGGSWAFHYDIAGDEDDEAGYRLGSHAFKEG
                     EYVSIKDQDGELQPFIVYSVEDV"
     gene            9360..10352
                     /locus_tag="PK812_04605"
     CDS             9360..10352
                     /locus_tag="PK812_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017486634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HRJ68869.1"
                     /translation="MRRDHVYAALDLGTNNCRLLVARPSWNGFRVVDAFSRIVRLGEG
                     LGSSRRLGPAAMDRTLDALKVCRDKIANRGVSRARFVTTEACRVAENGAEFLDRVERE
                     TGLALEVLDRSEEAHLAAAGCATLADRAAESIVLFDIGGGSTEIVWLRNGALAHAGTR
                     SRVAAWASLTAGVVTLAERHGGIHVTPDSFEDMVADVAEKLEEFASQAASAAEAENFH
                     LLGTSGTVTTLAGIHLGLARYDRRQVDGLWMSDGQIDYVINRLLAMSYDDRAANGCIG
                     SERADLVLPGCAIFEAIRRAFPSRRVRIADRGLREGILVELMQADGAWKGGPQR"
     gene            10349..11050
                     /locus_tag="PK812_04610"
     CDS             10349..11050
                     /locus_tag="PK812_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HRJ68870.1"
                     /translation="MSDGKTGRGEGGRRELAVRLKNARAHKPSSQQWLRRQLNDPYVS
                     RAKAEGYRSRAAYKFLEVDEKYRLLKPGMRIVDLGAAPGGWSQIAAKRVGSADGKGKV
                     VGIDLLDIEPIAGVDFAVMDFNDADAPERLQAMLGGPADGVLSDMAANTTGHKKTDHL
                     RIIGLAELAADFARQVLVPGGFFLIKLFQGGETGELVTALKRDFATVRHVKPAASRSD
                     SAELYVLATGFRGGS"
     gene            complement(11099..11737)
                     /locus_tag="PK812_04615"
     CDS             complement(11099..11737)
                     /locus_tag="PK812_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68871.1"
                     /translation="MPGLINLWSLLRLAPIVALLLLGLTACGQVPLATQWKLRGFDAL
                     GFDPALPRVAIVLTEAIRALPGGIRIRFRHGPKSAGDDLTEEVFVLQETAELPPVAHV
                     AGERPIVLRLDPADYPRIRTLQAQYAAFRHTAPDSRRVEIKVDAKGCRSADLSPARVP
                     ATIFLSADGNDFLPFLENLDLRGLTRAENKILEAEVPPCPPAPAAKKKVSRG"
     gene            11827..12405
                     /locus_tag="PK812_04620"
     CDS             11827..12405
                     /locus_tag="PK812_04620"
                     /inference="COORDINATES: protein motif:HMM:NF022636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HRJ68872.1"
                     /translation="MNAEAIGTPPGPQPDVNELRRRIGWICHVVRWSAVLYAVWIFWL
                     IGSFWSDGERVRRTYGFITGLPLTEPLAWQRAAGYGINALDWLLVVAAIFAVWQLMGE
                     YLRGRIFCPAAAIWMRRIGIFGSAAMVLDILCRPLTSAIMSLHMPEGARFIAVTVQPN
                     DLLILLFLLAFVALAHIFKSAAELAEDHAQIV"
     gene            12412..12627
                     /locus_tag="PK812_04625"
     CDS             12412..12627
                     /locus_tag="PK812_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRJ68873.1"
                     /translation="MAIIVNLDVMLARRKMRSRELAERIGLTEANVSLLKSGKVKGIR
                     FETLERICEVLDCQPGDILEFRDDSDR"
     gene            complement(12652..>13140)
                     /locus_tag="PK812_04630"
     CDS             complement(12652..>13140)
                     /locus_tag="PK812_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HRJ68874.1"
                     /translation="KGAWDRIKAEEKAEKAARRGKGADFVPQEQGVLAGVPTTLPGLT
                     RAVKLQDKAGKVGFDWNDPRAVIAKMREELDEVEQALDHGGAAERTEEIGDLLFVTAN
                     LARHLDVDPERALRGANAKFQSRFAHIERRLAALGRSPAQATLEEMDALWNEARAEDK
                     RA"
     assembly_gap    13141..13240
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(13253..13585)
                     /locus_tag="PK812_04635"
     CDS             complement(13253..13585)
                     /locus_tag="PK812_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68875.1"
                     /translation="MIQQNAKSDGPGDASQMTARVTTIISCPGVVPLAQSGTVVRPNA
                     NENHWYDRIGLRKRRYANLAGLAVVLALLFAGVWIMQEFVRLQKVAACYEAGRRDCAS
                     LSMERRGR"
     gene            complement(13716..14525)
                     /locus_tag="PK812_04640"
     CDS             complement(13716..14525)
                     /locus_tag="PK812_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRJ68876.1"
                     /translation="MNRGGTVTILGCGSSGGVPRVGQGWGACDPAEPRNRRRRCSILI
                     DFKGQGDALPTTVLVDTSPDLREQLLDADVKHLDAVVITHEHADHTHGLDDLRPLTMV
                     MRRRVDVFADRYATESLVNRFGYCFATPAGSPYPPILRLHTVECGRPLEIAGRGGSVE
                     FETFPLTHGEIEVLGLRFNKVAYTPDVSRIPDGSIAYLEHLDLWIIDALRPTPHPTHF
                     SLSEALDWIERLKPKRAILTNLHSDLDYGSLASKLPAHIVPAHDGMRIEFA"
     gene            complement(14522..15319)
                     /locus_tag="PK812_04645"
     CDS             complement(14522..15319)
                     /locus_tag="PK812_04645"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRJ68877.1"
                     /translation="MLVDTHCHLDFPDLSGDISGVIARARANGVNRMVTISTRVRKFA
                     TYGALADSHAEVFCTVGTHPHNAAEEPDITAEELIRLSQHPKCVGIGEAGLDYHYDKS
                     PRDTQARSFRVHIAAARATGLPLVIHARAADDDMITILEEEHAQGAFPAILHCFSSGP
                     ELARRGLALGFYLSFSGILTFKASEEVRAIAAASPIDRLLVETDAPYLAPVPHRGKKN
                     EPAFVRHTAEMLAEVKGVSTGEVARITTDNALRVFSKMPPLPAREAA"
BASE COUNT         2708 a         4922 c         5057 g         2758 t
ORIGIN      
        1 cacatcatcg aagccaacca ggccagcgag cacttcttcg atctgagccg cgcgcttctg
       61 gcgcggcgca gcctttccga tctcgttccg gctggttcgc cgctgctggc gctgatcgag
      121 caggtgcgcc agcgcaacgc cggcattacg gaatatgcag tcgatctcgt gacgcagcgg
      181 gcgattgccg aacgcctcac cggcgagcgt ctgctggacc tctatgccag ccccgtcgcg
      241 aaccgtgccg gacacgtgat tgtcgtgctg caagagcggt cgatggccga caagatgact
      301 cgccagctca cgcaccgcgg cgcggcgcgg tcggtttcgg cgatgtccgc catgctggca
      361 cacgagatca agaacccgct gtccggcatt cgcggcgcgg cgcaattgct ggaaatcgac
      421 gcggatgaca ccgcgcggga actgacagga ctgatccggg cggaggtgga tcggatcgtt
      481 cgcctggtcg accggttcga ggtctttgcc gacggcgcgc ctgccccctt cgcgccgatc
      541 aacatccacg aggtgctgga tcatgtcatc cggctggcgg cgagcgggtt cgcgcgcgat
      601 attcgcgtta ccaaggacta cgatccttcg ctgccgcccg tgtttggcaa cggtgatcag
      661 ctcgttcagg tcatgctgaa cctgatcaag aatgcggccg aggcgattca cgacttccgg
      721 gaggatggag aaatcaccgt atccaccgcc tatcggccag gcgtgcgcgt tctggtgcca
      781 tcgagcggcg aacgcatcag cctgccgctg gaagtctgcg tcgtcgacaa cgggcccggc
      841 attcgcgaaa gcctgatcga acacctgttc gaacccttcg tcaccaccaa ggcttccgga
      901 actggcctgg gtcttgcaac ggtggcgaag atcgtaagcg accacggcgg cgttgtggaa
      961 tgcgagcggg tgaagcgcgg aaccatgttc cgcatcctcc tgccgctgca cagaccggcc
     1021 gctgtccgtc gcgaagcagg aagccgaacg gatgcagaat agcgccaaga ccattctgat
     1081 cgcggatgac gacgccgcga tccgcactgt cgtcatgcag gccctgactc gggcaggcta
     1141 ctcggtgcgc tcgaccggaa ccgccggcgt tctgtggcgc tgggtatcgg caggggatgg
     1201 cgacctcgtg atcacagatg tggtgatgcc ggatgagaac atctttgatg tgctgccccg
     1261 gatccgccgg gcgcgtccgg acctgccagt catcgtcatg agcgcgcaga acaccttcat
     1321 gacggcgatc aaggcctcgg agcgcggcgc ttatgactac ctgcccaagc cgtttgacct
     1381 gaacgaactg ctcagtctcg tccgccgggc attggccgcc gccgcgcccg ccaaacccgc
     1441 tcagccggcc cagcagaacg acgagatccc tctcgtcggt cgctctccgg ccatgcagga
     1501 gatctaccgc gtccttgcgc ggctgatgca gaccgacctg accgttatca tcacgggcga
     1561 atccggcacc ggcaaggaac tggtggcgcg cgcgctgcac gatttcggca agcgtcggcg
     1621 cggaccgttc gtggctgtca acatggccgc gatcccgaaa gacctgatcg aaagcgaatt
     1681 gttcggccat gaaaaggggg ctttcaccgg cgcgaacacg cgcagcgccg ggcgtttcga
     1741 acaggctgaa ggcggcacgc tgtttctcga cgagatcggc gacatgccga tggaagcaca
     1801 gacccgtctg ctgcgcgtgc tgcagcaggg cgagtacacc acagtgggcg ggcgcacccc
     1861 gatccgcacc gacgtgcgca tcgtcgccgc gaccaacaag gacctgaccg ttctcatcga
     1921 gcagggcctg ttccgcgagg acctctacta ccggctcaac gtggtgccga tccgcctgcc
     1981 ggccctgcgc gagcgcgcgg cggatatccc cgacctcgcg cgccatttcc tgatgctcgc
     2041 cgagaagggc gggttggctc acaagacctt caacggctcg gctcttcagc agctccagca
     2101 ctatccctgg cccgggaacg tgcgggaact cgaaaatctc gtgcgtcgcc tggtcgctct
     2161 ctacccggat gaggtcatca cggcggaaat ggtcgagaat gaactcggcg aggcgcgacg
     2221 tctgacaccc tctgccggcc cctcggccca cccccagccg ccagccgccc aggcacgcgg
     2281 tgcagcggcg tttcagggcg gcagcaccga tacgatcgaa tccttcctgg aacggcacct
     2341 ggtcgattac ttcgccagtt tcggttcgga tctgccgcct ccggggctgt atgatcgggt
     2401 cttgcgggag gtggagcgac cgctcgtcgc ggcggcgctc ggcgcaaccg gcggcaacca
     2461 ggtgaaagcc gccgatttgc tcggcgtcaa tcgcaacacc ctgcgcaaga agatcaagga
     2521 cctcggcatt cggctggtga gcacgatccg ctaacccgtg gtcttggcgt ttcggccatg
     2581 attgcgcggg cagcgggccg tagccgaaga tttgtcacat ttcgacaaca gtgttgcaaa
     2641 gactcggcac ttgcctacgg atggcgatcc gggaatcggt cagtcgacag ggcaggatga
     2701 acagcgtcac cgagatacca cagcttgacc cccctgtcgg cagggcgcgg cggctgctgg
     2761 gctatgcgga gatcgggctg ttcggagctg ccctggtggc ggctgctgcc accatcgcga
     2821 tcctgatggg cgtcggcgtt cgtcccagcg aaaccgcgtt catcgtcgcg ttggctgcgg
     2881 acatcctgct cgcactgttg ttggtggcgc tggttggcgt gcgactctgg gcgctcaacc
     2941 gggcgcgcaa gcacggacag gccggcgctc gtctgcattt gcgcatcgtc acgctgcttg
     3001 gcctcgtcgc cggacttccg acggcgctcg ttgcgctgtt cgccatgatc acgctggagc
     3061 gcggcctgaa cccgtggttc tccggcgacc tcaagatcct ggtcgagaac tccgacaaga
     3121 tcgcgcgtca gttccagcag cagctttgcc agaacgttgt ccgtgagatg cgcctgatgg
     3181 cggtcgacgt cgagcgtgcc gtcaccagcg gcatctacca gtctgaccgc aagctgttcc
     3241 gcgagtacat gacctcgcgc gcggttttcc tcagcctgcc gttctcggcc atcatcaagc
     3301 cggacgggca ggttctcgaa cgggccgaac tcggtacgtc tctcggcgaa ttcacgccga
     3361 atgcggagga tttcgcgcag gccgccaccg aggagccgcc gtgcatcttt tcccagcaga
     3421 cgctcgggac tctgaccaag ctgcgcggct ttgacgacgg ctatctcatc gtcggccgtc
     3481 ccatcgaccg ccgcgcgatc gagtttccgc tgatcgcgca ggccggagtc caacagtacc
     3541 agactctgga ttcccgtcgc gcggcgacgc agttgggcgt cggcgtcgtc ttcgtgctga
     3601 tcacgctgat cgtgcttctg gcggcgagcc tgctcggatt gcgctttgcc gaccgacttg
     3661 tgcaaccggt ccgccggctg atcatggcga cagacgccgt gtcctccggc aatctctatg
     3721 tgcaggttcc ggtcggcacc gaagactccg acattgccca tctcggctcg acattcaaca
     3781 acatgacatc ggttcttcgg tcgcagcaca acagcctgct tgccgcgaac cagttgaacg
     3841 accagcgacg ccggttcatg gaagccgtgt tgtccggtgt cccggcgggt gtcgtcggca
     3901 tcgacgggct gggacgcatc agcatcgcca atcccaccgc ggagcgactg ctcggggcag
     3961 agccgggcgc tctcaccggc cgggagcttg tggacgtcct gccgcagatc aagtccgtgc
     4021 tggacgaggc acgcattggc gcgcaccgcg tgcttcagga aacgatctcc ctgtcgcagg
     4081 cggggcgcga ccgtaccctg tcggtccgca tcgcccatga agcgtccggc gacgacgaga
     4141 tgggactcgt ggtcacgctc gatgacatca ccgatctcgt gaccgcacaa cgcacttccg
     4201 cctgggcgga tgtggcccgg cgcattgccc acgagatcaa gaacccgctg acgccgatcc
     4261 agctttccgc ggaacgcatc cggcgaaaat tcggcaaggt catcgccgag gacaaggcga
     4321 tcttcgacca gtgcaccgac acaatcgtgc gtcaggtcga ggacatcaag cgcatggtcg
     4381 acgagttctc ctcgttcgcg cgcatgccca agccacgccc ggagagcgag gatatcgtgg
     4441 cggtgatcaa ggaaaccgcc ttcatgatgc gggtggcgca tcccgatatc gtgatcgagg
     4501 agcacctgcc ggctaagtct gtcagggcga cgttcgaccg tcggctgatc gctcaggcca
     4561 tgcagaacat catcaagaac gccgctgagg caatcgctgc cgttccgaat ggtgagcgcg
     4621 gcccggggcg gatcgaactg cgcctgtttc atcccgagcc ggaccgcgtg gccatcgatg
     4681 tcatcgacaa cggcaagggg ttcccgagcg agaaccgcga ccgcctgctg gagccgtaca
     4741 tgacgacgcg cgaaagcggg acggggcttg gtctcgcaat tgtcgcaaag atcctggaag
     4801 agcacggcgg cggcatcgaa ttgcgcgatg cgccgtcggg caggggcgcc tgcgtgcgcc
     4861 tgttactccc ttccgcagcc gttgccactc cggcagcaca ccctgctcaa ccgacggata
     4921 ggcatgcatg agccaggaaa tactcatcgt cgacgacgag tccgacatcc gcgaactcgt
     4981 cgccggcatc cttgaggatg agggctacgc gacgcgcagc gcccgaaacg ccgatgaggc
     5041 gatagcggcc atcgagtcgc gccggccaca tctcctgttc ctcgacatct ggcttcaggg
     5101 cagccgtctc gatggcttgc agttgcttga agtcatcaag cagagccatc cggaactgcc
     5161 cgttgtgatg atttcggggc acggcaatat cgagacggcc gtttccgcca tcaagagcgg
     5221 agcctacgac ttcatcgaga agccgttcaa ggccgaccgg ctggtgctgg tggcgtcccg
     5281 cgccctcgaa gcccagcaga tgaagcgcga gattcagtcg ctgaagacgc ggtcgggcca
     5341 gaccgaccgc cttgtcggcc ggtcagcggc catgaaccag ctgcgccaga ccatcgatcg
     5401 catcgccccc accaacgcgc gtatcctgat ttccggagcc cctggcgcag gcaaggaggc
     5461 ggtggcgcgg gccatccatg gcgcttcgtt gcgtgcgtca gggccattcg tggtcaccaa
     5521 cgcggcgacg atcacgcccg agcgcatgga agaggcgctt ttcggcgtcg agggcggccc
     5581 gaaccagaag cgtcgcaacg gggcgctgga ggacgcgcac ggcggcacgc tgttcatcga
     5641 cgaaatcgcc gacatgccga aggaaaccca gagcaagatc ctgcgggtcc tggttgatca
     5701 gaacttccag cgcgttggcg gcgcgacgcg tgtctttgtc gatgtgagaa tcatttcctc
     5761 cacttcgcgg gatcttaagg ccgagatcac ggccgggcat ttccgcgagg atttgtttca
     5821 tcgcctcgcc gtcgttccac tcctggttcc cggcctctcg gagcggcgcg aggacattcc
     5881 cgaactgatc gacgtcttca tggaccagat ttccatggcc accggtctgc cgaagcgcct
     5941 gatcggtccg gacgcgctgg cgatcctcca gacgcatgac tggcccggca acgtgcgcca
     6001 gctgcgcaac aatatcgagc gtctgatgat tctcgccgcc ggcgatcctt ccacggtggt
     6061 gacggccgag atgctgccgc aggacgtcgg gtccgcggct cctccgacac cggccggggc
     6121 aggcggagaa cgcctgcttg ggctgccgtt gcgcgaggcg cgggaaatct tcgagcgcga
     6181 gtatctgatt gctcaggtca accgttttgg cggaaacatc tcccgaacag ccgagttcgt
     6241 cggcatggaa cgctccgccc tgcaccgcaa actcaagtcg ctcggtgtct agggtgaaat
     6301 ctcaacagaa tggctgacat gcggattcag tgcttgctgc gatgcggtca gttgtatgat
     6361 catgtttgtg ttgcggtgca atcgggtggt gtcgacagcg gcagccaccc ccggctccgg
     6421 ataaccgcag gcgtcctgtg accttcgggt cgggcgactg ccggttccgg cgcgaaggac
     6481 agaggcaaga aaatggcgaa tgaacgaggg cagaatctcc aggacgtgtt cctgaaccat
     6541 gtccggaaga acaaaatccc cctgacgatc ttccttgtga atggtgtcaa gctgcagggt
     6601 gtcgtcacgt ggttcgacaa cttctgcgtt ctgctgcgcc gggatggcgt gtcccaactg
     6661 gtctacaaac acgcgatctc gaccatcatg cccggcgccc cggtcacgct gtcgagcccg
     6721 atggaagatg aggcttgatg gccgtcccag ccgcaggcgg cgcatgacgt gaaggacagg
     6781 cgaacgccgg acctgccaag cttcgacgct gcctcggcgg cagagcagcg ggttgccgct
     6841 catacccgca cgcttgtcgt cggtccttac ctgcaggaac gcgccgccgc gagggcgggt
     6901 gaaaccgcga cgcgaggctt cgaggcgcgg ctcgacgagg ctgtcggcct tgccggcgcc
     6961 atcgacctga acatcgtgcg tgccgaaatc gccccactca agacgccgcg accggcgacc
     7021 tatctcggcg tgggcaagat tgaacaattc aaggcgctgg tcgagcagga ggacatcgcc
     7081 ctcgtgctgg ctgactgcgc gctttcgccg gtgcagcagc gcaatctcga aaaggcgctg
     7141 ggcgccaagg tgatcgaccg gaccggactg atcctggaaa tttttggccg gcgcgcccgc
     7201 acgcgggaag gagcgcttca ggttgaactc gcgcacctgc actaccagaa gagccgactt
     7261 gtccgctctt ggacccacct ggaacgccag cgcggtggct tcggctttct cggcggtccg
     7321 ggcgaaacgc agatcgaggc cgaccgccgc ctgatccagg agcgcatgaa gcgaatcgag
     7381 cgcgaactgg aagacgtcag ccgcacccgc ggactgcatc gcgccagccg ggacaaggtg
     7441 ccgtttccga ccgtcgctct ggttggctat accaacgccg gcaagtcgac actgttcaac
     7501 cgcatgacgc gggccgaagt cctggccaag gacatgctgt tcgccaccct cgacccgacg
     7561 accagggtca tcgagctgcc ccatggcaag aaggccatcc tttcggacac ggtgggcttc
     7621 atttccgacc tgccgaccat gctggtcgcg gcgttccggg caacccttga ggacgtcatt
     7681 ctcgccgatc tcatcctgca tgtgcgcgat gtggcgcacg aggacgcgga agcgcaaggc
     7741 gcggatgtgc gccggattct cggcgaactc ggcatcgacg aggcacagtc cgcccgggtc
     7801 ctggaggtct ggaacaaggc ggacctgctg ccaccggacg agcgcgagcg gctgctcggc
     7861 ctttcggccc gggaacgacc gggggagggg cggccagtgc ttgtttcagc cctgaccggc
     7921 gagggaatcg cgcaacttca ggcggtgatc gaagagcgcc tgtcgcaggg gcacgagcgt
     7981 cggcaggtgc tcatgcccgg atcggacggc ggtgggatcg cctggatcta cgaaaactgc
     8041 gaggtgctgg ggcgcgagga tcgcgacgat ggcaccgttg cgctcgacat ccggctgtcg
     8101 ccggaacgtg tcgagcggtt cgagcgccgg atcaggccgc tttcgggcgc ttgaccgggg
     8161 cagggcggga cgcttcggcc gtccggagcg gctccatgta gtagtcttca ccgccggcca
     8221 gatcgctgaa tccgccatcg ccattgctcc agagccgcga aagcttgagc accggcttgt
     8281 ctgcgagggc aggccgttgg gtttggacga aaggtgctcg cgacgtgcct tgcatgatgc
     8341 actcctgaga tgtggacgcc cagacggaag tcacgggcga cggatcattg cggcgagttt
     8401 gcggatttcg gcgctcagcc caagaaggcg ctcgcgcgag gcagcgtcca tgtcggtctc
     8461 gatatcgctg cgcatcgcgt tgagagcctc gcaggacgaa cggatcgcgt cgagattgtc
     8521 gggcaggcgc ggcagggcgc tccccggtgc ttccggacgg caattggacg cggcagacat
     8581 gatgcacagc cccgattggt tgcggcgcag tgaagcgcaa agcctttcaa ggtgcgctaa
     8641 ccagaccgct acaattctcg ggacatgcgc atttccgtcg ggcgcgggac cgcccgattc
     8701 cgacgcggcg cgcagaagtg ctagacgtcc tcgacgctgt agacgatgaa cggctgcaac
     8761 tccccgtcct ggtccttgat cgagacgtat tcgccttcct tgaaggcatg ggagcccagc
     8821 cggtatccgg cctcgtcgtc ctcatcgccg gcgatgtcgt agtggaaagc ccagctgccg
     8881 cccggccgac gggtcaggtg gccgaggtca tcctcgtcgt tcgcccagaa gcgcttgacg
     8941 cggcactgct cgcgcacctt gtgccaggct tcggcatcga tatgggcatc ggcgtcgagc
     9001 ggcgcgacaa attcataccc gtgcttgtag gacccgtttg gaaagtcctt tgtgcgggca
     9061 agatgcaggg taatgcgctg gagtttcgat gcggacatgg atgcctcgtt gaaagcgatg
     9121 cgcaaatctt tcgtgccccg gctcttcgcg ccttgctttg gatcaagcgc tctgatgggg
     9181 cggggagcgg gaggtcgccg caatcggcct tccctttctt gcgcgaacac ggcacagtat
     9241 gcgcgaaagg actttcgatg acgatcggtc cacccgagag agattcgaac cgaccgccat
     9301 cgccggcaac gaacagggac tacggaacca ggattgcgac ggatcgccag ccatgcgcga
     9361 tgcggcgtga ccatgtgtat gcggcgctgg acctaggtac gaacaattgt cgtctgctcg
     9421 tcgcgcgacc gagctggaat ggctttcgcg tggtcgatgc cttttcacgc atcgttcggc
     9481 tgggcgaggg acttggttcg agccgccgcc ttggccccgc ggcaatggat cggacgctgg
     9541 acgcgctgaa agtttgccgc gacaagatcg cgaatcgcgg ggtatcgcgc gcccgtttcg
     9601 tcaccaccga ggcctgtagg gtagcggaga acggagcgga attccttgat cgcgtcgagc
     9661 gcgagacggg cctggccctg gaagtgctgg accgctcgga ggaggcgcac cttgccgccg
     9721 ccggctgcgc caccctggcg gaccgcgcgg cggaaagcat cgtcctgttc gacattggcg
     9781 gcggttccac ggagatcgtc tggttgcgca atggcgcgct ggcccacgcc ggcacgaggt
     9841 cgcgcgtggc ggcctgggcc tcgctcacgg cgggtgtggt gaccttggcc gagcgccacg
     9901 gcgggattca tgtcaccccc gacagcttcg aggacatggt ggcggacgtg gccgagaagc
     9961 tggaggaatt cgccagccag gccgcctctg ccgccgaagc ggagaatttc cacctgctgg
    10021 gcacctctgg caccgtgacc acgctggccg gcatccatct cggcctcgcg cgctatgatc
    10081 gccgccaggt cgacgggctg tggatgtccg acgggcagat cgactacgtc atcaaccgtc
    10141 tgctcgccat gtcctatgat gaccgcgcgg cgaacggctg catcggctcg gaacgggccg
    10201 acctcgtgct gccgggctgc gccattttcg aagccatccg ccgggccttc ccaagccggc
    10261 gggtgcggat cgcggatcgg ggcctgcgcg agggtatcct cgtcgaactg atgcaggccg
    10321 atggcgcctg gaagggcggt ccgcaacgat gagtgacggc aagacagggc gtggcgaggg
    10381 cggacgacgc gagctggcgg tgcgcttgaa gaacgcccgc gcgcacaagc catcctccca
    10441 gcaatggctg cgccggcagc tcaacgaccc ctacgtgtcg cgtgccaagg cggaaggcta
    10501 ccgctcccgc gcggcctaca agttcctcga agtcgacgaa aagtaccgct tgctcaagcc
    10561 tggcatgcgc atcgtagatc tcggcgcagc gcccggcggc tggtcgcaga tcgccgcgaa
    10621 gcgtgttggc agcgccgacg gcaagggcaa ggtcgtgggt atcgatctgc tcgacatcga
    10681 gccgatcgcc ggcgtcgatt tcgcggtcat ggacttcaac gacgccgatg cgcccgagcg
    10741 cctccaggca atgctcggcg gcccggccga cggggtgctg tccgatatgg cggccaacac
    10801 cacaggtcac aagaagaccg atcacctgcg catcatcggg ctggcggaac tggccgcaga
    10861 cttcgcgcgg caggtgctgg tgcccggcgg ctttttcctg atcaaactgt tccagggcgg
    10921 cgaaaccggc gaactcgtca cggcgctcaa gcgcgacttc gccacggtcc gccacgtgaa
    10981 accggccgcc agccggtcgg attctgctga attatacgtg ttggcaacgg gttttcgcgg
    11041 cgggtcatag cgcatttcgc tccccggccc gtcgccgcat gtcgcaagcg atgccgggtc
    11101 atcctcgcga gaccttcttc tttgcagccg gcgcaggagg acagggcgga acttccgctt
    11161 ccagaatctt gttttccgca cgggtgaggc cgcgcagatc gaggttttcg agaaatggca
    11221 ggaagtcgtt cccgtccgcg ctcaggaaga ttgtcgccgg aacgcgcgcc ggcgacaggt
    11281 cggcgctgcg gcaccccttg gcgtccacct tgatctcgac gcggcgcgag tccggcgcgg
    11341 tgtgccggaa ggcggcgtat tgggcctgta gcgtccgaat tcgcggataa tccgctggat
    11401 caagccgaag gacaatcggg cgctctccag caacatgcgc gaccggcggc aattcggcgg
    11461 tttcctgcag gacaaacact tcttcggtca ggtcgtcccc tgccgacttc ggcccatgcc
    11521 ggaaccggat gcgtatgccg ccgggcaggg ctcggatcgc ctcggtgagc acgatggcaa
    11581 cgcgcggcag ggcagggtcg aagcccagcg catcgaaacc gcgcagtttc cattgtgtcg
    11641 ccagcggaac ctggccgcag gctgttaggc cgagcaacag caaggcgacg atcggagcca
    11701 gtcgcaggag gctccacaga ttgatgagac cgggcatgct ttctctttca tggaaaataa
    11761 ttattgtttg acaataaaaa tttatcgact agatttggct tgtcaatggg tagacggaac
    11821 ttttcgatga acgcagaagc catcggaacg ccaccaggac cgcagccgga cgttaacgag
    11881 ctgcgccgtc gcatcggctg gatctgccat gtcgtgcgtt ggtccgcggt tttgtatgca
    11941 gtgtggattt tctggctgat cggcagcttc tggtcggacg gggagagggt gcggcgcacc
    12001 tatggcttca tcaccggcct gccgctgacc gagcctctgg cctggcagcg cgccgccgga
    12061 tacggcatca acgcgctcga ctggttgctg gtcgtggcgg cgattttcgc ggtctggcaa
    12121 ttgatggggg agtatctgcg cggccgcatc ttttgtcccg ctgcggcgat ctggatgcgc
    12181 aggatcggga tattcggcag cgccgcgatg gtgctcgata tcctctgccg gccgctgacg
    12241 agcgccatca tgtccctgca catgccggag ggggcgcggt tcattgccgt gacggtgcag
    12301 ccgaacgacc tgttgatcct tttgttcctg ctcgccttcg tggctctggc gcacattttc
    12361 aagagcgctg cggaactggc cgaagatcac gcgcagatcg tgtgaggccc tgtggcgatc
    12421 atcgtcaacc tggatgtcat gctcgcccgc cgcaagatgc gctcgcgcga acttgccgag
    12481 cgcatcggcc ttaccgaggc caatgtgtcg ttgctgaaat ccggaaaggt gaaaggcatc
    12541 cgcttcgaaa cgctcgaacg catttgcgag gtgctggact gccagcccgg cgatatcctc
    12601 gagtttcgcg acgatagcga ccgataggcg cactttcgtc gttgccgctt ttcaggcccg
    12661 tttgtcctcg gcccgcgcct cgttccagag cgcgtccatt tcttccagcg tcgcctgtgc
    12721 cggagagcgg ccgagggcgg caagccggcg ctcgatatgg gcaaaacggc tctggaattt
    12781 cgcgttggcg ccccgcagcg cgcgttccgg atcaacatcg agatggcgag ccaggttggc
    12841 ggtgacgaac agcaggtcgc cgatttcctc ggtccgttcg gcagcgccgc cgtggtccag
    12901 cgcctgctcg acctcgtcga gttcctcgcg catcttggcg atgacggcgc gcgggtcgtt
    12961 ccagtcgaat ccgaccttgc cggccttgtc ctgcagcttc acggcgcgag tgaggccggg
    13021 cagggtggtt ggaacccccg ccagcacgcc ctgctcctgc ggaacgaaat ccgcaccctt
    13081 gcccctgcgc gccgccttct ccgccttttc ctcggccttg atgcgatccc acgcgccttt
    13141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gaccttgctc gctcagcgac
    13261 cgcgtcgctc catgctcaga gacgcgcaat cccgccggcc ggcttcataa catgctgcca
    13321 ctttttgcag ccgcacgaac tcctgcatga tccacacgcc cgcgaacagc agagccagca
    13381 ccaccgccag accggcgagg ttggcgtaac ggcgcttgcg caggccgata cggtcgtacc
    13441 aatggttctc gttggcgttg ggacgcacca cggtgccgct ttgagccagc ggcacaacgc
    13501 ccggacagga aatgatcgtg gtgacgcgcg cggtcatctg cgacgcgtct cctggaccat
    13561 ccgacttggc gttctgctga atcatggcgc gcagcgctcc ggcgacggaa gcatcactat
    13621 gcgcaatcgg atggcgtaat ccagtgcttt ttcgcgacag atcgccggca ttgtggccgc
    13681 gatggctcac aatcccgcgt ctgacttgac atgtgtcagg cgaattcgat gcgcatgcca
    13741 tcatgcgcgg gcacaatgtg cgccggcagt ttgctggcca ggctgccgta gtcgaggtcg
    13801 gaatgcagat tggtcaggat ggcgcgcttg ggcttcagcc gctcgatcca gtccagcgcc
    13861 tcggaaagcg agaaatgcgt cgggtgcggc gttgggcgaa gtgcgtcgat gatccacagg
    13921 tcgagatgtt ccagataggc gatagaaccg tccggaatgc gggaaacatc aggggtatag
    13981 gcaactttgt tgaagcgaag tccgagcacc tcgatttcgc cgtgggtgag cggaaatgtc
    14041 tcgaattcca cggacccgcc acgcccggcg atctccagcg gcctgccgca ctccaccgtg
    14101 tggagccgaa ggatcggcgg atacgggctg ccggccggcg tggcgaagca atagccgaag
    14161 cggttgacca gcgattccgt ggcgtagcga tcggcgaaca cgtcgacgcg tcggcgcatg
    14221 accatggtca gcgggcgcag gtcatcaaga ccgtgcgtat ggtccgcatg ctcgtgggtg
    14281 atgacgacgg cgtccagatg tttcacatcc gcgtcgagca gctgttcgcg caggtccggc
    14341 gaggtatcga ccagcacggt agtgggcagg gcgtccccct gccccttgaa atcgatcagg
    14401 atcgagcagc ggcggcgacg gttcctgggc tccgccggat cgcaggcgcc ccagccctgg
    14461 ccgacgcgtg gcaccccgcc tgaggagccg cagccgagaa tggtgacggt tccgcccctg
    14521 ttcatgccgc ttcccgcgcc ggtagcggcg gcatcttcga aaagacgcgc agggcattgt
    14581 ccgtggtgat ccgggcgacc tcgccggtgc tgacgccttt cacctcggcc agcatctcgg
    14641 cggtatggcg cacgaaggcc ggctcgttct tcttgccgcg atgcggtacg ggcgcgagat
    14701 acggcgcgtc cgtctcgacc agcaaccggt cgatgggcga ggcggctgcg atcgcgcgca
    14761 cctcctcgga ggccttgaat gtcaggatgc cggagaagga caaatagaag ccgagcgcca
    14821 gaccgcgccg ggccagctcc ggcccggagg agaagcaatg caggatggcc ggaaacgcac
    14881 cctgtgcatg ttcctcttcc aggatcgtga tcatgtcgtc atccgccgcc cgggcatgga
    14941 tgaccagcgg cagtccggtg gcgcgcgccg ccgcgatatg cacgcggaac gagcgcgcct
    15001 gggtgtcgcg cgggctcttg tcgtagtggt agtccagacc ggcctcgccg atgccgacgc
    15061 acttggggtg ctgtgacagc cggatcagct cctcggcggt gatgtccggt tcctccgccg
    15121 cgttgtgggg atgtgtgccg accgtgcaga acacctccgc atgggaatcc gccagggctc
    15181 cgtaagtcgc gaacttcctg acacgggtcg agatcgtcac catgcggttg acgccattgg
    15241 cacgggcgcg ggcgatcacc ccggaaatgt caccggaaag atcgggaaaa tcgaggtggc
    15301 aatgggtgtc gacgagcatg gcagcggcag gaatattcgg ttggcccgca ctagcgggag
    15361 cagggcatca tggcaacagc atgcgctgaa cacgcacgcg tatcagttcc ggacctcagg
    15421 cggtcgaccc taccctttcg cagccccctc ctccgcctct acgaaacggg ggaaaatgcc
    15481 gctcggtgcc ggcaggtttg cgcccgagac cagccggccg gccggtccaa gcgccgcgaa
    15541 gccgc
//
LOCUS       DAOMXN010000033        14775 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_2243_length_14775_cov_11.992867, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000033 DAOMXN010000000
VERSION     DAOMXN010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 14775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14775
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2243_length_14775_cov_11.992867"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..812
                     /gene="lptB"
                     /locus_tag="PK812_04650"
     CDS             3..812
                     /gene="lptB"
                     /locus_tag="PK812_04650"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HRJ68878.1"
                     /translation="MPAPHPAPSGQQPHDSLAWVKPQRQADEGQDILSAHQLGKAYRG
                     REVVKGASLYVRKGESVGLLGPNGAGKTTIFYMITGLVPADKGQIVLDGHDITPLPMY
                     RRARLGIGYLPQEASIFRGLTVEENVRAVLEVVEPDKKRREKELDALLEEFNIARLRK
                     SPSIALSGGERRRCEIARALAGRPSFMLLDEPFAGIDPIAVGDIQALVRQLTARGIGV
                     LITDHNVRETLGLIDRAYIIHSGQVLMEGSPDEIIANPDVRRLYLGEDFRL"
     gene            complement(825..2057)
                     /locus_tag="PK812_04655"
     CDS             complement(825..2057)
                     /locus_tag="PK812_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012253321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HRJ68879.1"
                     /translation="METVLIEWGGLALRWLHIITGMAWIGSSFYFMHIDAHMRAVPDI
                     AKGGETWEVHGGGFYQVRKYLVAPEKLPEELIWHKWQSYSTWMSGFLLLCWIYYFSAD
                     LYLIDPAVRDLTQIQAILIGIVALAAGWLLYDFLCNSKLGDNEVLLAAIGFAIVMAFA
                     YGFQQVFSGRGALIHTGALMATIMSGNVFFNIIPNQRKVIADLIAGRTPNPEFGKQAK
                     KRSTHNNYLTLPVVFLMLSNHYPLTYSSPYSWVIIGMVLVAGALIRVFYNFRHAGKGS
                     PYWAWAVAILLMLAAAYISAASSPGGREHLGLGPMPEEKALAGRAEAPKEVAEIIQSR
                     CSMCHAREPVWRGIGVAPKGVRLETPADVAANRELIRIQTVATRAMPPNNITSMTQAE
                     RKIVGQWLASARPVAAKQ"
     gene            complement(2167..4518)
                     /locus_tag="PK812_04660"
     CDS             complement(2167..4518)
                     /locus_tag="PK812_04660"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HRJ68880.1"
                     /translation="MAPSIPEERIALAGLKAPAEIAIDRWGIPHLRAQSLDDLFCVQG
                     FNAARDRLFQLDLWRKRGLGLLAGDFGPGYLEQDRASRTFLYRGDMTAEYARYGDDTQ
                     AICEAFVRGVNAFIALAERRPEHMPDEFALLGTKPARWAAEDVLRIRTHALSQNALSE
                     IARAVILSRADAATDALRKTIEPPVALDPKLDLSAIPASAADLFKLATAGVTLSRERL
                     QATLAEAGRWSIVNDLGEVQLREHTDGSNNWAIHGSRTASGRPILGSDPHRAHSVPSL
                     RYLVHLTAPGFDAIGAGEPALPGISLGHNGTIAFGLTIFGADLEDVFVYETHPDDADA
                     YRYGAGFERMRIVEERVPVKGHADQKVRMKFTRHGPVVHEDPSRRLAIAIRSVWQEPG
                     SAAYAASLATMRARDYESFRSALRRWGAPSVNMVYADVEGNIAWMPAGFNPKRTNWNG
                     LTPVPGDGSHEWDGMVDPDALPRVRNPDRGFVYSANEMNLPADWPHDQIRVGHEWIEA
                     SRANRIREVLSGTESHTLGHTTALQTDVTSLPARRLLALLRQVSARTAEEAAALAMFT
                     GWDCGLEADSAPAALFEVWFARHLKPALFALLVPDAGVRALLMPGDVEGILRALERPD
                     RRFGESPESGRNALLLSSLAAAYADCAARMGKDTAQWQWGRLHHGYFEHVVSRLLPES
                     GRGRFDVGPLPKGGSASTPMHTGYRPSDFRILHGASVRLAIDVGGWDNSLCLNAPGQS
                     GDPKSPHYRDLAPLWAGGRYVPLLYSRSEVDKATIRRIVLEPG"
     gene            complement(4707..6185)
                     /locus_tag="PK812_04665"
     CDS             complement(4707..6185)
                     /locus_tag="PK812_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007697804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRJ68881.1"
                     /translation="MAAMTAAVLIAQPARAQYARETFVVAMPGDAATLDPHLHWDTDS
                     YMVYRNVFDNLVTRDPEGRIVPQVATGWRYLDDRTIEFDLRMNIRFHDGSPLTVDDVV
                     FSIRRVTDPALKSPQLSQFDQIDTVEAVGQAKMRMKTKSTYPALMAQLVKMSIVPKAY
                     VEKVGDQKFNLEPMGSGPYRFKSWQKGIGITLEANDTYWGGRPPFRTLAFRLVPDLST
                     RVADLKTGKADLARGLTPDQAQQVKADRNLQVLSGPTERIGYLFINAMQPPTDDRRVR
                     QALAYAIDRKTIIEALLQGYANPVEVMLTPASTGYVDDVKGYPFDPVRARALIKEAGA
                     EGKTLTFLTSPVYDARIVQAIQQMLSDVGLKVEIQTMDQPTFLRRRQGRPDEAGNLSL
                     GRWSCACQDADGVIFPMFRSGSPWSKYANPAFDAEVDAARVIIDESRRKTHYRRAFEI
                     LNEDMASLPLYQDYAIYAARRELRWKPTPAEALTVMNMQWVP"
     gene            complement(6242..7180)
                     /locus_tag="PK812_04670"
     CDS             complement(6242..7180)
                     /locus_tag="PK812_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007768026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HRJ68882.1"
                     /translation="MSAQSDNPPWSWPEERWRKAVNHVRAGRSLKPKKWKGGARCAVA
                     LSFDADHETIELRFGGESFGKLSQGQYGARQGTPRILNVLRRHGVKATFFMPAVSALI
                     NPDEARQVVKEGHEIGIHSWIHEFNSTLPYEAERELALRAREVLSRITGSAPVGMRTA
                     SWDFSPHTLKIIREMDLLYDSSLMADDDPYELLDEGQPTGIVELPVEWIRDDAVYFNM
                     DRMAALRPYTAPEAVFDIFRRELEAAYDDGGLYVLTMHPHVIGHRSRLWILEEIIKLA
                     KSLPGVWFATHAEIAAFCKKTAGLQVPPPPAPQPPV"
     gene            7293..8189
                     /locus_tag="PK812_04675"
     CDS             7293..8189
                     /locus_tag="PK812_04675"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRJ68883.1"
                     /translation="MSLRDQTSKAQARPADNLRGAALLVLAMLALTLEAAAIRWVGPS
                     ASVSQAVFFRAMAQLLVVCCWSLWRGGLPDLRTGRPWLHVARGAGSVSSWWLYYYTFR
                     HLDFALATILTFATSLFVVLLARPILGEKVRLGSWVATLIGFGGVALASGVGTVGVDP
                     TVAVGLSAALIGSLVVFLNRTLSQSEDMLTIFTYICFFVVAAAIPMAWYDWQPISLAQ
                     TAILLGAGLLGALGMLLTIEAYGRGETSVLAPIPFVRIVFAMAFAYLLFAETPTLNMV
                     VGAVVVVGAALAVAAGERRPPR"
     gene            complement(8202..9128)
                     /locus_tag="PK812_04680"
     CDS             complement(8202..9128)
                     /locus_tag="PK812_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HRJ68884.1"
                     /translation="MFGMVEGTGTEEQDPRFRACLISHARVERLWTGARWSEGPAWFG
                     AGRYLVWSDIPNNRMLRFDETDGSVSVFRQPSNNSNGNTVDLQGRLITCEHQARRVTR
                     TEHDGSITVIADRYHGKRFNSPNDVVVKSDGSIWFTDPDYGIWTDYEGDKAESEIGGC
                     NVYRVDPSGSVSLVADDFERPNGLAFSPGEDHLFVSDTGISHRHGGPRHIRRLKVKPD
                     GKRLGASKVFAECAAKVFDGFRFDADGRLWTSAGDGVHCYHADGTLIGKIRIPEVVAN
                     VTFGGPKRNRLFICGTTSLYSMYLAINGAKTA"
     gene            complement(9178..9735)
                     /locus_tag="PK812_04685"
     CDS             complement(9178..9735)
                     /locus_tag="PK812_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ActR/PrrA/RegA family redox response regulator
                     transcription factor"
                     /protein_id="HRJ68885.1"
                     /translation="MAEFQGTPDLPADNSLLIVDDDRPFLTRLARAMESRGYSVRMAS
                     SVAEGMAAIAAAAPAFAVIDMRLADGSGLDVMQELKRCRPEARAIILTGYGNIATAVT
                     AVKLGAFDYLAKPADADDIDAALKAGSVVKPELPQNPMSADRVRWEHIQRVYESCDRN
                     VSETARQLNMHRRTLQRILAKRAPR"
     gene            complement(9823..11139)
                     /locus_tag="PK812_04690"
     CDS             complement(9823..11139)
                     /locus_tag="PK812_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ActS/PrrB/RegB family redox-sensitive histidine
                     kinase"
                     /protein_id="HRJ68886.1"
                     /translation="MTRDADAMLHASQHLRLRLDTLIRLRWLAIAGQSLAVMFVEFVL
                     GFPTPLVGTGIVILVATVLNVALGVAYPKGHRLDGRSSALILAFDILQLSALLYLTGG
                     LENPFSYLFLAPVMISATVLSPRMTIALGALAVACASVLATWHLPLPWLAGQTLVVPG
                     IYKAGVWVSILLGIAFIGVYAWRVADEARQLADALAATELVLTREQHLTMVDGLAAAA
                     AHQLGTPLATIAVVARELERQVGPEHPFREDVALLRTEAARCREILGKIASLGGEDSG
                     PHGELTLGHLLEELVAPHRNFGVEIDVRLDGEGAVPSCARNPGVIYGIGNLVENAVDF
                     AAARVTVAASWSADTVEVRVLDDGPGFGAGVLDRLGDPYVTSRGGERGEKRGEGGGLG
                     LGLFIARTLLERSGASVTWGNRPGVSGASVHMVWPRRLFEARQTPS"
     gene            11360..12649
                     /gene="phaZ"
                     /locus_tag="PK812_04695"
     CDS             11360..12649
                     /gene="phaZ"
                     /locus_tag="PK812_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HRJ68887.1"
                     /translation="MNRNMYYFYETAHSVISPSRAIVDAIGFFYRNPLNPLSQTPFGR
                     NVSAACELYERLTRRYGKPAFALPTTEIDGQTVHITETVVWEKPFCKLLRFERSLHPA
                     TAGQSKLLIVAPMSGHYATLLRGTVEAFLPTHDVYITDWADARMVPLSQGNFDLDDYI
                     DYVMEILRLLGPAVHVVAVCQPSVPVLAAIARLEALNDPSAPISMTLMGGPIDTRQSP
                     TEVNKLAEKRGIEWFRSNCIMRVPFPNPGFMREVYPGFLQLSGFMAMNIDKHVSAHWE
                     MFRHLTEGDGDNADKHRDFYDEYMAVCDLTAEFYLQTVDKVFVKHQLPKGEMMHRDAP
                     VDLKAIRRVALMTVEGEKDDISGVGQTKATHDLCVNIPKDRQLYHLQSKVGHYGVFNG
                     SRFKAEIVPAMNRFYAAMERSVPAAPARPRLVASRKG"
     gene            12698..13489
                     /locus_tag="PK812_04700"
     CDS             12698..13489
                     /locus_tag="PK812_04700"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003604516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HRJ68888.1"
                     /translation="MFTWPPRLLRPAVPAPPKEERFEVAHEGSVYAVSVRRSAAARRL
                     ILRVRNDTGDVVLTVPKRLSLSCAKDFATRQGGWIAARVARLPDRQPFVPGAVIPLRG
                     VLHRLALRDGLRGAARIEPAGADGTPGLSIACDPAHFSRRVLDFLRAEAKRDLTAASR
                     RYAERLQVSIARISIKDTRSRWGSCSSAGALSYSWRMIMAPPHVLDYLAAHELAHRIE
                     LNHSQRFWKTVAAICPHWREAENWLTRHGSGLHRYGPAGGRPTGL"
     gene            13512..14702
                     /locus_tag="PK812_04705"
     CDS             13512..14702
                     /locus_tag="PK812_04705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HRJ68889.1"
                     /translation="MARLLPNTLGLTLLLALVTSTNPIGTDMYLPSLPAIRESLQAST
                     AEAQATLSAFLVGAVLAQLVLGPWSDYVGRRPAILLGLVLFVAGSIACALAPSIEVLI
                     GARVLQSFGGAGAAVIGRSMVRDLFSGWKAGKEFARLNSIRGVVPLVCPLLGAALTGW
                     FGWRSNFVAMAVFGLPLIVVVGIALPETLAQKPDKPFSVSGAVADYRDILTTPSFLFY
                     AGLNALVFSGFFAFLSGSAFVMQGLYGMSKTEYALGFSAVVGGYIAGSMIAHRLQARF
                     APQSLIFGGLAATTLASFAMLCLMVADVGGALAVIITMALFTTGMGTVMPYCEAGALR
                     PFSDRVGSAASMTSIVNMSVAALFGLAVGQIIDASAMALPAALMGAAAVALALFVRFR
                     SRFA"
BASE COUNT         2421 a         4819 c         4816 g         2719 t
ORIGIN      
        1 cggtgccggc gccgcacccc gcgccgtccg ggcagcagcc gcatgactcc cttgcctggg
       61 tgaagccgca gcgacaggcg gacgaggggc aggacattct ttccgcacac cagctcggca
      121 aggcctatcg cggccgcgag gtggtgaaag gcgcaagcct ctatgtccgc aagggcgaat
      181 ctgtcgggtt gctcggcccc aacggcgcgg gcaagaccac gatcttctac atgatcaccg
      241 ggctggttcc ggcggacaag ggtcagatcg tgctggatgg gcacgacatt acgccgctgc
      301 cgatgtatcg ccgcgcgcgg ctcggcatcg gctacctgcc gcaggaggcc tcgattttcc
      361 gtggcctcac ggtcgaagag aacgttcgcg ccgtcctcga ggtcgtcgaa ccggacaaga
      421 agcgcagaga aaaggaactc gatgcgctgc tggaagagtt caatatcgcc cggctgcgca
      481 agtcgccgtc cattgcgctg tccggtggcg agcggcgtcg ctgcgagatc gcgcgggcgc
      541 tggcagggag gccgtccttc atgctgctgg acgagccctt cgcgggcatc gatcccatcg
      601 cggttggcga catccaggcg ttggtgcgcc agctcaccgc gcgcggcatt ggcgtgctca
      661 tcaccgatca caacgtgcgc gagacgctcg gcctcatcga ccgcgcatac atcatccact
      721 ccggtcaggt gctgatggaa ggctcgccgg acgaaatcat cgccaatccc gacgtgcgtc
      781 gtctctacct cggcgaagat ttccggctct gagcaaatgc cgggttattg cttcgctgcc
      841 accgggcgcg ctgaagcaag ccactgcccg acgatcttgc gctcggcctg cgtcatcgac
      901 gtgatgttgt tgggcggcat ggcccgggtc gcaacagtct ggatacggat cagctcgcga
      961 ttcgccgcca cgtcggccgg cgtttccagg cgcacgcctt tgggagcgac gccgatcccc
     1021 cgccagaccg gttcgcgcgc atgacacatc gaacagcggc tctggatgat ctcggccacc
     1081 tctttgggcg cctcggcccg tcccgcgagg gctttttcct ccggcatggg gccgaggccg
     1141 agatgttccc ggccgccggg tgacgatgcg gcgctgatgt aggcggccgc gagcatcaac
     1201 aggatcgcca ccgcccaggc ccagtagggg ctgcccttgc cggcatggcg gaagttgtag
     1261 aacacgcgaa tcagcgcgcc ggccaccaga accatcccga tgatcaccca ggaatagggc
     1321 gaggagtagg tcagcgggta gtggttcgac agcatcagga agacgaccgg cagggtcagg
     1381 taattgttgt gggtggaccg cttcttggcc tgcttgccga actcgggatt gggggtacgt
     1441 ccggcgatca ggtcggcgat caccttgcgc tggttcggga tgatgttgaa gaacacgttc
     1501 ccggacatga tcgtcgccat cagggcgccg gtatggatca gcgcgccgcg cccggaaaag
     1561 acctgctgga acccgtaggc gaaggccatg acgatggcga agccgatcgc ggccagcagc
     1621 acttcattgt cgccgagctt cgaattgcac aggaaatcat agagcagcca gccggcggcc
     1681 agcgcgacga tgccgatcag gatcgcctgg atctgggtca ggtcccgaac ggccggatcg
     1741 atcaggtaca ggtcggcgct gaagtagtag atccagcaca ggagcagaaa gccggacatc
     1801 caggtcgaat aggactgcca cttgtgccag atcagctctt ccggcagctt ctccggtgcc
     1861 acgaggtact tgcgcacttg gtagaatccg ccgccatgca cttcccacgt ctcaccgccc
     1921 ttggcgatat cgggcaccgc ccgcatatgg gcatcgatgt gcatgaaata gaaggacgaa
     1981 ccgatccagg ccatgccggt gatgatgtgc agccagcgca gtgccagccc gccccactcg
     2041 atgagcaccg tctccatgct tgtccgtccc tgatccgtcg atttgccaca ggatggcacg
     2101 aacgcacgtg ggattacagg tccccgtttt gagagctgct gcgaaatttc aagacaatcg
     2161 cgcccctcag cccggttcca gcacgatgcg ccggatggtt gccttgtcga cttcggaccg
     2221 gctgtagagc agcgggacat agcggccacc cgcccagagc ggcgcgagat cccgataatg
     2281 cgggctcttt ggatcgccgg actggcccgg ggcgttgagg cacaggctgt tgtcccagcc
     2341 gccgacatcg atggcgagcc ggacagaggc gccatgcagg atgcggaagt cacttggccg
     2401 atagccggtg tgcatgggag tcgaagcgct tccacccttt ggcagcgggc cgacatcgaa
     2461 gcggccgcgc ccggattccg gcagcaggcg ggacaccaca tgttcgaaat agccatgatg
     2521 caggcggccc cactgccact gcgcggtgtc cttccccatc ctggccgcac agtcggcata
     2581 ggcggcagcg agggaggaca ggagcagggc gttgcggccg ctttcgggac tttcgccgaa
     2641 cctccggtct ggacgttcca gggcgcgcag gatgccttcg acgtcgcccg gcatgagcag
     2701 ggcgcgcacg cctgcatccg gcaccagcag ggcgaacagc gccggcttga gatgcctggc
     2761 gaaccagacc tcgaacagtg cagccggcgc gctgtccgct tccagcccgc aatcccaacc
     2821 ggtgaacatc gccagcgcgg cggcctcctc ggcggtgcgc gccgacacct gtcgcagcag
     2881 cgccaggagc cgccgcgccg gcagcgacgt gacgtcggtt tgcagcgctg tcgtatggcc
     2941 aagcgtatgg ctctcggtac cggacaggac ctcacgaatg cggttggcac gcgaagcctc
     3001 gatccactcg tggccgacac ggatctggtc gtgcggccag tcggccggaa ggttcatctc
     3061 attggcggag tagacaaagc cccgatcggg gttcctgacg cgcggcagcg catcgggatc
     3121 gaccatgccg tcccattcgt gcgagccgtc gcccggaact ggcgtcagcc cgttccagtt
     3181 cgtccgcttc ggattgaagc cggccggcat ccaggcgatg tttccctcga catcggcata
     3241 gaccatattg accgaagggg cgccccagcg gcgcagcgcg gagcggaaag actcgtaatc
     3301 gcgcgcccgc atggtggcga gtgaggccgc ataggcggcg gaccccggtt cctgccagac
     3361 actgcggatg gcgatcgcca accgccggga cgggtcctcg tgaaccaccg gtccgtgccg
     3421 ggtgaacttc atgcgaactt tctggtccgc atggcccttg accggaacgc gctcctcgac
     3481 gatccgcatc cgttcgaacc ccgcgccgta gcgataggcg tcggcatcat ccggatgcgt
     3541 ctcgtagacg aaaacatctt ccagatcggc gccgaagatc gtcagcccga aggcgatcgt
     3601 tccattgtgg ccaagcgata tgccgggcag ggccggttct ccggcgccga tcgcgtcgaa
     3661 tcccggcgcc gtgaggtgca cgaggtagcg aaggctcggc accgaatggg cgcggtgtgg
     3721 gtccgacccg aggatcggcc ggccgctggc cgtgcgggac ccgtggatcg cccagttgtt
     3781 cgagccgtcg gtgtgctccc gcagctgaac ttcgccgaga tcgttgacaa tggaccagcg
     3841 cccggcctcc gccagcgtcg cctggagccg ttcccggctc agcgtcacgc cggccgttgc
     3901 gagcttgaac aggtcagcgg ccgatgccgg gattgcggaa agatcgagct ttggatcaag
     3961 ggccaccggc ggctcgatgg tcttgcgcag ggcgtccgtc gcggcatcgg cgcgggacag
     4021 tatgaccgcg cgcgcgattt cggacagcgc attctgcgag agcgcatgcg tgcggatgcg
     4081 caggacatcc tccgctgccc atcgcgccgg cttggttccg agcagcgcga actcgtccgg
     4141 catgtgctcg ggccggcgct cggccagcgc gatgaaggca ttgacgcccc tgacgaaggc
     4201 ttcgcagatc gcctgcgtgt cgtcgccata gcgcgcgtac tccgcggtca tatcgccgcg
     4261 atacaggaag gttcggcttg cgcgatcctg ctccagatag cctgggccga aatcgccggc
     4321 cagcaggccg aggccgcgct tgcgccacag atcgagctgg aacagccggt cgcgcgccgc
     4381 gttgaaaccc tgcacgcaga acaggtcatc cagggattgc gcgcgcagat gcggtatgcc
     4441 ccagcggtca atggcgattt cggccggcgc cttcagtccg gccagggcga tgcgttcttc
     4501 ggggatggac ggagccatga tgctgggccg cttgtcgaat gcagcgtcgt ggggggcatc
     4561 gcgctagcag ccgcgctgct tccggcttcc ggcgatcggg gcgccatgta accagacctt
     4621 cagcctctgg aacagcacgc catggcaaaa tcgaagcgcg accggtcgcc tcccgcgccg
     4681 ggtcccgatc ctgtcctcgg ccgtgctcag ggcacccact gcatgttcat cacggtcagg
     4741 gcttccgcag gcgtcggttt ccatcgcaat tcccggcggg ccgcgtagat ggcgtagtcc
     4801 tgatagagcg gcaggctggc catatcctcg ttcaggatct cgaacgccct gcggtagtgg
     4861 gtcttgcgcc ggctctcgtc gatgatgacg cgcgccgcgt cgacctcggc gtcaaaggcc
     4921 ggattggcgt atttcgacca ggggctgccg cttcggaaca tcgggaagat cacaccatcg
     4981 gcatcctggc aggcgcagga ccagcgcccg agcgacaggt ttccggcctc atccggacgc
     5041 ccctgacgcc ggcgaaggaa ggtcggctgg tccattgtct ggatctcgac cttcaggccg
     5101 acgtcggaca gcatctgctg gatcgcctgc acgatgcgcg catcgtatac cggcgaggtc
     5161 aggaaggtca gtgtcttgcc ctctgcgcca gcctccttga tcagcgccct ggcgcggacc
     5221 ggatcgaacg gatagccttt cacatcgtcc acatatcctg tgctcgccgg cgtcagcatc
     5281 acctcgacag ggttcgcata cccctgcagc agcgcctcga tgatggtctt gcggtcgatg
     5341 gcataggcca gggcctgcct gacgcgccgg tcatcggtcg ggggctgcat cgcgttgatg
     5401 aaaagatagc cgatgcgctc ggtcggtccg gacagaacct ggagattgcg atccgccttg
     5461 acctgctggg cctgatcggg cgtgagaccg cgcgcgagat ccgccttgcc ggtcttgagg
     5521 tccgcgacac gcgtggacag gtcgggcacg agacggaagg cgagcgtccg gaacggcggc
     5581 ctgccgcccc agtaggtgtc gttcgcctca agggtgatgc cgatgccctt ctgccaggac
     5641 ttgaaccggt aggggccaga ccccatcggc tccaggttga atttctggtc gccgaccttc
     5701 tcgacatagg cctttggcac gatggacatc ttgaccagct gggccatcag ggccggatag
     5761 gtcgacttcg tcttcatccg catctttgcc tgcccgacag cctcgaccgt atcgatctgg
     5821 tcgaactgtg aaagctgcgg gctcttcagg gccggatcgg tgacgcgccg gatcgagaac
     5881 accacatcgt ccacggtcag cggcgagcca tcgtggaagc gaatgttcat gcgcaggtcg
     5941 aactcgatcg tccggtcgtc gaggtatcgc cagcccgttg ccacctgcgg cacgatcctg
     6001 ccttccgggt cgcgggtgac gaggttgtcg aaaacattgc ggtagaccat gtagctgtcc
     6061 gtatcccaat ggagatgcgg atcgagcgtc gcggcatcgc cgggcatggc gacaacgaac
     6121 gtttccctgg cgtattgcgc ccgggctggc tgggcgatca gcacggccgc ggtcatcgcg
     6181 gccatgagca atgtccgtat catggggccc cccttctgcc gcatcgggct gtggcgtgcg
     6241 atcagaccgg cggctgaggt gctggcggtg gcgggacctg gagcccggcg gttttcttgc
     6301 agaatgcggc gatctcggca tgggtcgcga accagacccc tggcaggctc ttcgccagct
     6361 tgatgatctc ctcgagaatc cagaggcgcg agcggtggcc gatgacatgg ggatgcatcg
     6421 tcagcacgta gagaccgccg tcgtcgtagg ccgcctccaa ctcgcggcga aagatgtcga
     6481 ataccgcttc cggcgcggtg taggggcgca gggctgccat ccggtccatg ttgaaataga
     6541 ccgcgtcatc gcgaatccac tcgaccggga gctccacgat ccccgtaggc tgcccttcgt
     6601 cgagcagttc gtagggatcg tcgtccgcca tcagcgagga atcgtacaag aggtccatct
     6661 cgcggatgat tttcagcgtg tgcggcgaga aatcccagga cgccgtgcgc atgccgaccg
     6721 gcgccgaacc ggtgatccgc gacagcactt cacgcgcgcg cagtgcgagt tcgcgctcgg
     6781 cttcgtaggg caatgtcgaa ttgaattcgt ggatccagct gtggatgccg atctcgtgcc
     6841 cttccttcac cacctgacgg gcctcgtccg gattgatcag ggcggacacc gccggcatga
     6901 agaacgtcgc cttcacaccg tggcggcgca gcacattcag gatgcgcggc gttccctgcc
     6961 gcgcgccata ctggccctgg ctgagcttgc cgaacgattc gccgccgaac ctcaattcga
     7021 tggtctcgtg gtcggcatcg aaggacagcg cgacggcgca gcgcgcgccg cctttccatt
     7081 tcttgggttt cagcgagcgt ccggcgcgca catggttgac cgccttgcgc cagcgttcct
     7141 ccggccagct ccagggcgga ttgtccgact gcgccgacat tcttgcctcc cgtgcccttg
     7201 acgcccttga agtccccatc ctgatgcgaa atgcttagag acagaaggat ttcaaccggc
     7261 gcgtcctgcg tccaggcgca ggcccatcgc acatgtccct acgcgaccag acctcgaagg
     7321 cgcaagctcg acctgcggac aacctgcgcg gggccgcgct gctggtgctg gcgatgctgg
     7381 ctctcacgct cgaagcggcg gcgatccgat gggttggtcc ttccgccagc gtctcgcagg
     7441 ccgtcttctt ccgcgcgatg gcgcaactgc tggtcgtttg ctgctggtcg ctctggcgcg
     7501 gaggtttgcc tgacctgcgc accggccgtc cctggctcca tgtggcgcgc ggggccggca
     7561 gcgtcagcag ctggtggctc tattattaca ccttccggca tctcgacttc gccctggcga
     7621 cgatcctgac cttcgcaaca tcgctgttcg tcgtgcttct cgcccggccg atcctcggcg
     7681 agaaggtccg cctcggtagc tgggtcgcga ccctgatcgg ttttggcggc gtggccctgg
     7741 catccggcgt cggtacggtg ggtgtcgatc cgacagtcgc ggtcggtctc tcggccgcgc
     7801 tgatcggatc gctggtggtg tttctcaatc gcaccctgtc ccaatccgag gacatgctga
     7861 cgatcttcac ctacatctgt tttttcgtcg tcgcggcagc cattccgatg gcctggtatg
     7921 actggcagcc gatcagcttg gcgcagacag ccatcttgct gggtgcgggc ctgttgggcg
     7981 cgcttggcat gctgctgacc atcgaagcct acggccgcgg cgaaacatcc gtgctcgccc
     8041 cgatcccttt tgtccggatc gtgttcgcga tggccttcgc ctatctgctt tttgccgaaa
     8101 cgccgaccct gaacatggtt gtcggcgcgg ttgtcgtggt gggtgcggcg cttgcggtcg
     8161 ctgccggcga gcggcgccct ccgcgctgag cgccatcaca gctaggcggt cttcgccccg
     8221 ttgatggcca ggtacatcga atagagcgat gtagttccgc agatgaacag gcggttgcgc
     8281 ttggggccgc cgaacgtcac gttcgcgacc acttccggga tccggatctt gccgatcagc
     8341 gttccatccg cgtgatagca gtgcactccg tcgccggcgc tggtccagag cctgccgtcg
     8401 gcgtcgaagc ggaagccgtc gaagaccttg gcggcgcact cggcgaacac cttggaggca
     8461 ccgagtctct tgccgtccgg tttcactttc aagcggcgga tgtgacgggg accgccatgc
     8521 cgatggctga tgccggtgtc cgagacgaaa aggtggtcct cgcccggaga gaaggcgagc
     8581 ccgttcggcc gttcgaaatc gtccgccaca agcgagacag agcccgacgg gtccacccga
     8641 tagacgttgc agccgccgat ttcgctttcc gccttgtccc cttcatagtc ggtccagatg
     8701 ccgtagtcgg ggtccgtgaa ccagatcgag ccgtcggact tcaccacgac gtcgttcggg
     8761 gaattgaagc gcttgccgtg gtagcgatcg gcgatcaccg tgatcgaacc atcgtgctcc
     8821 gtgcgcgtca cacgtcgtgc ctgatgctcg caggtgatca ggcgcccctg caaatcgacg
     8881 gtattgccgt tcgagttgtt cgagggctgg cggaacaccg aaaccgagcc gtcggtctcg
     8941 tcgaaccgca gcatccggtt gttgggaatg tccgaccaga cgagatagcg tcccgcgccg
     9001 aaccaggccg gcccctcgct ccagcgcgcg ccggtccaca ggcgctccac ccgggcatgc
     9061 gaaatcaggc aggcacgaaa ccgtgggtcc tgctcctctg ttcccgtgcc ttcgaccatt
     9121 ccgaacatct tgcttccccg gggtgccttc gactgggcgc gatgtggtgt gcgatgtcta
     9181 gcgcggcgca cgcttcgcga ggatgcgttg aagcgtgcgg cgatgcatgt tgagctgacg
     9241 cgcggtttcg gacacgttgc gatcacagga ttcgtacacg cgctggatgt gttcccagcg
     9301 cacccggtcg gcggacatcg ggttttgcgg cagttccggc ttgacgacgc tgccagcctt
     9361 gagcgcggca tcgatgtcgt ccgcgtcggc tggcttggcc agatagtcga aggcgccgag
     9421 cttcacggcg gttacggccg tggctatgtt gccgtagccg gtcaggataa tggcccgtgc
     9481 ctcgggacgg cagcgtttca gttcctgcat gacgtccagg ccgctgccgt cggccaggcg
     9541 catgtcgatc acggcgaaag ccggtgcagc cgccgcgatc gccgccatgc cttcggcgac
     9601 gctcgacgcc atgcgcacgc tgtatccccg gctttccatg gcgcgtgcca gacgcgtgag
     9661 aaagggacgg tcgtcgtcga cgatcaacag gctgttgtcc gcgggaaggt cgggggtacc
     9721 ctgaaactcg gccatgatcg cgtccgtctg tccaatgctt ctccataagc ccatggcgcg
     9781 gcaaatgtca cccccgcagc cgcggccggg ccggggcaag gctcagctcg gtgtctgccg
     9841 cgcctcgaac aggcggcgtg gccacaccat gtggaccgaa gcgccggaca cgcccggccg
     9901 gttgccccag gtgacgctgg cgcccgaacg ttcaagcagc gtgcgcgcga tgaaaagccc
     9961 gagtccgagg ccgccgccct cgccccgttt ctccccgcgc tcgccgccac gggaggtgac
    10021 gtaggggtcg cccagccggt caagcacgcc cgcgccgaag cccggaccgt catccagcac
    10081 gcggacctca accgtgtccg ccgaccagga tgcggccacg gtcacgcgtg ccgcggcgaa
    10141 atccaccgcg ttctcgacca gattgccaat tccgtagatc acgccgggat tgcgtgcgca
    10201 ggagggaacc gcgccctcgc cgtccagcct gacatcgatc tccacgccga aattgcggtg
    10261 cggcgcgacc agttcctcaa gcaggtgtcc gagggtcaat tcgccatggg ggcctgaatc
    10321 ctctccgccc aacgaggcga tcttgcccag aatctccctg caccgcgcgg cttcggtccg
    10381 cagcagcgcg acatcctcgc ggaacggatg ttccggaccg acctgccgtt ccagttcgcg
    10441 cgccacgacc gctatggtgg ccagcggcgt gccgagctga tgcgctgcgg cggcggccag
    10501 tccgtccacc atcgtcaggt gctgttcgcg ggtgagaacg agttccgtgg cggccagcgc
    10561 atcggccagc tggcgcgctt catcggcgac acgccaggca tagacgccga tgaacgcgat
    10621 accgagaagg atcgaaaccc atacgcccgc cttgtagatc ccgggcacca ccagggtctg
    10681 gccggcgagc cagggcaggg gcaggtgcca ggtcgcaaga acggaggcgc aggcgacggc
    10741 gagcgcgccg agcgcaatcg tcatgcgcgg ggaaagtacc gtcgctgaaa tcatgaccgg
    10801 tgccaggaac agataggaaa acgggttttc cagcccgccg gtcaggtaga gcagcgcgga
    10861 cagctgcagg atgtcgaatg caaggatcag ggcggaggat cgcccgtcga gcctgtgccc
    10921 cttggggtag gcgacgccga gtgccacgtt cagcacggtc gcgaccagga tcacgatccc
    10981 cgtcccgacg agcggggttg gaaatcctag gacgaattcc acgaacatca cggcaagcga
    11041 ctggccggcg atcgccagcc agcgcagccg gatcagtgtg tccagccgca gccggagatg
    11101 ctgcgaagca tggagcatgg catcggcgtc gcgggtcacg atggcggtcc cgccttcgcc
    11161 gattggcgcg gtgcgaaagg aattttctct gctattcccg aggcttgttg catttatgtc
    11221 gcattgcagc aatagtttaa tggattctgc atgatcgatg gtgttcaatt gcctccggac
    11281 tcgtcaatct caccatccga cagacaaggg ccgccacctg cttcggtagg cgcgccacag
    11341 gggcagcgag aggcaatcaa tgaatcgcaa tatgtactat ttctacgaga ccgcgcactc
    11401 ggtgatcagc ccgtcgcgcg ccatcgtgga cgcgatcggt tttttctatc gaaaccccct
    11461 caacccgctg tcgcagaccc ctttcggccg caatgtttcg gccgcctgcg aattgtatga
    11521 gcgcctgacc cgacgttacg gcaaaccggc tttcgccctg ccgacaacgg aaatcgacgg
    11581 gcagaccgtg cacatcaccg aaacggtggt gtgggagaag ccgttctgca agctgctgcg
    11641 attcgagcgt tcgctgcacc cggcgacggc cggccagtcc aagctgctga tcgtcgcgcc
    11701 gatgtccggt cactatgcca cgctgctgcg aggcacggtc gaggctttcc tgccgacgca
    11761 cgacgtctac atcacggact gggccgacgc ccgcatggtg ccgttgtccc aggggaactt
    11821 cgacctcgac gactatatcg attacgtgat ggaaatcctg cgtctgctgg ggccggccgt
    11881 gcatgtggtg gcggtatgcc agccgtcggt tccggtcctg gcggcgattg ccaggctgga
    11941 ggctctgaac gatccgagtg cgcccatttc gatgaccttg atgggggggc ccatcgatac
    12001 gcgccagagc ccgaccgagg tgaacaagct ggccgagaag cgcggcatcg aatggttccg
    12061 tagcaactgc atcatgcggg tgccgttccc caatccgggc ttcatgcgcg aggtctatcc
    12121 cggcttcctg caattgtccg gcttcatggc catgaacatc gacaagcacg tctccgcgca
    12181 ctgggagatg ttccgccacc tgaccgaggg cgacggcgac aacgccgaca agcatcgcga
    12241 tttctacgac gagtatatgg cggtctgcga cctgactgcg gagttctatc tccagaccgt
    12301 cgacaaggtc ttcgtgaaac accagctgcc gaagggcgag atgatgcatc gcgatgcgcc
    12361 ggtcgacctg aaggccatcc ggcgtgtcgc gctcatgacg gtcgagggcg agaaggacga
    12421 tatctcgggc gtcggacaga ccaaggcgac ccatgatctg tgcgtcaaca ttcccaagga
    12481 tcgccagctc taccacctgc agtcgaaagt cggccattac ggcgtgttca acgggtcgcg
    12541 cttcaaggcg gagatcgttc cggccatgaa ccggttttat gccgcgatgg agcgcagcgt
    12601 tccggcggcg cccgcgcgtc cgcgcctcgt cgcctcgcgc aagggctgat cccgccgttc
    12661 tggccggggc gcatcgaatc ttctaagcct tggggccatg ttcacctggc caccccggct
    12721 cctgcgcccc gctgtgcccg cgccgccgaa ggaagagcgg ttcgaggtag cgcatgaggg
    12781 ttccgtctat gccgtaagcg tccgtcgcag cgcggccgcg cggcgcctga tcctacgggt
    12841 ccggaacgac accggcgatg tcgtcctgac cgtgcccaaa cgcctcagcc tgtcctgcgc
    12901 caaggatttc gccacccgtc agggcggctg gattgccgcg cgcgtggcgc gcctgccgga
    12961 tcgtcagcca ttcgttccag gcgccgtcat ccccctgcgc ggtgtcctcc accggctggc
    13021 tttgcgggac gggctgcggg gcgccgcccg catcgaaccg gccggggcgg atggaacgcc
    13081 tggcctgtcg attgcctgcg atcccgctca cttttcgcgg cgggtgctcg attttctcag
    13141 ggccgaggcc aagcgcgatc tgaccgcagc ctcgcgccgc tatgccgagc gtctccaggt
    13201 ttcgatcgcg cggatctcga tcaaggacac ccgcagccgc tggggctcgt gttcctcggc
    13261 cggagccctg tcgtactcct ggcgcatgat catggcgcca ccccatgtcc tggactacct
    13321 cgcggcccat gaactggcgc accgcatcga acttaaccac tcgcaacggt tctggaaaac
    13381 cgtggctgcc atctgccccc attggcgcga ggcggaaaac tggttgactc gccacggttc
    13441 cgggttgcat cgctacggtc ccgccggcgg tcgaccaacc ggcctttgac attcaagcga
    13501 gaccggcgtc cttggccagg ctgctcccca acaccctcgg tctgacgttg ctgctggcgc
    13561 tggtcacctc gaccaatccc atcggcacgg acatgtatct tccatcgctg ccggcgatcc
    13621 gcgagtcgct ccaggcctca accgccgagg cgcaggcgac cctttcggcg tttctcgttg
    13681 gcgcggtgct ggcccagctc gtcctcggtc catggtcgga ctatgtcggc cggcggccgg
    13741 cgatccttct tggcctggtg ctcttcgtgg caggcagtat cgcctgcgcc ctggcgccca
    13801 gcatcgaggt cctgatcggc gcgcgcgtgc tgcagtcctt cggcggtgcg ggcgcggcgg
    13861 tgatcgggcg gtcgatggtg cgcgatctgt tttccggctg gaaagcgggc aaggaattcg
    13921 cgcggctcaa ttccattcgc ggcgtggtgc cgctcgtctg tccgctcctc ggggcggcgc
    13981 tgacgggctg gttcggctgg agatcgaact tcgtggccat ggccgtgttt ggactgccct
    14041 tgatcgtcgt tgtcggcatc gccttgccgg aaacgctggc gcagaagccg gacaagccct
    14101 tttccgtcag cggcgctgtc gccgactatc gcgacattct gaccacgcca tctttcctgt
    14161 tctacgccgg gctgaatgcg ttggtgtttt ccggcttctt cgcgtttctg tccggttcgg
    14221 ccttcgtgat gcagggcctc tatgggatga gcaaaactga gtacgctctc gggttctcgg
    14281 ccgtggtggg tggctacatt gcaggttcga tgattgccca ccgcctgcag gcccggttcg
    14341 ctccgcaatc gctgatcttc ggcggtctgg cggctacgac tctggcctcg ttcgcgatgc
    14401 tgtgcctgat ggtcgcggat gtgggcgggg cgctcgccgt gatcatcacc atggccctgt
    14461 tcaccaccgg catgggaacg gtcatgccct attgcgaagc cggggccttg cgccccttct
    14521 cggatcgcgt cggcagtgcc gcctcgatga cgtccatcgt caacatgtcg gtggcggcgc
    14581 tgttcggtct cgccgtcggt cagatcatcg atgcgtcggc catggcgctt cctgcagcgc
    14641 tgatgggtgc cgcagccgtg gcgctcgccc tgttcgtgcg gttccgcagc cgcttcgcct
    14701 gagcggacgt cagcggctga gcaactggtt gagccgttcg cgatcactcg tggacgggcc
    14761 gggcactgat cgcgg
//
LOCUS       DAOMXN010000034        14213 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_2344_length_14213_cov_12.114705, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000034 DAOMXN010000000
VERSION     DAOMXN010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 14213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14213
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2344_length_14213_cov_12.114705"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            167..1600
                     /locus_tag="PK812_04710"
     CDS             167..1600
                     /locus_tag="PK812_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homospermidine synthase"
                     /protein_id="HRJ68890.1"
                     /translation="MNDWPVHGTITGPIVMIGFGSIGKGTLPLIERHFRYDRARLVVI
                     DPEDKDRPLLDELGVRFIHRAVTRDNYRELLKPLLTEGGGQGFCVNLSVDTSSVDIMT
                     FCQEIGALYIDTVNEPWAGFYFNLSAGPAARSNYALRETTLAAARSTRGKPTAVSCCG
                     ANPGMVSWFVKQALLNVATDLGLSFEEPKTREDWARLMQACGVKGVHIAERDTQRSRN
                     PKPLNVFVNTWSVEGFVSEGMQPAELGWGTHEAWMPENGRTHETGCGAAIYLLQPGAN
                     TRVRSWCPTPGAQYGFLVTHNESISIADYFTVRDASGKAVYRPTCHYAYHPANDAVLS
                     LHEMFGAEGRRQPTWHILDENEIVDGIDELGVLLYGHGKNAYWYGSQLSIEETRRIAP
                     YQNATGLQVTSAVLAGMVWALENPNAGIVEADEMDFRRCLEVQLPYLGPVIGAYTDWT
                     PLDNRGKLFSEDVDTADPWQFRNILVR"
     gene            complement(1655..2173)
                     /locus_tag="PK812_04715"
     CDS             complement(1655..2173)
                     /locus_tag="PK812_04715"
                     /inference="COORDINATES: protein motif:HMM:NF022605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2937 family protein"
                     /protein_id="HRJ68891.1"
                     /translation="MIARLLTSAMVVVGGVSAVQLPEFAQQYRQRLGGATDELRAIVE
                     RFDADARSEGLSRAEALRKHTENAEELFRKRGAAMIDTIARQQRLERFRDDMAKLSGG
                     NLTMALATRLDRETARSTFNDYEPAIPFTGEGGVLAILGGFVGWILARLLGWPKRAIA
                     RRLQARKETHYA"
     gene            complement(2227..3225)
                     /locus_tag="PK812_04720"
     CDS             complement(2227..3225)
                     /locus_tag="PK812_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003609095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HRJ68892.1"
                     /translation="MTAASFETLTQHVADLPAGDFVAHAAFLGKVPVLAMSDGRIRIG
                     DASGAREVQAHDATILVAADDATRLVTGGDDGKVIAIDASGSLATLADEKGRWIDAVA
                     LGASGATAWSVGKRVNARDGKGRVKSLDLPSSSRGLCFAPKGYRLAATHANGTTLWFP
                     AVSAEPERLEWKGSHLDVTWSPDGRFVVSSMQENQLHGWKLPEKTHMRMSGYPAKTRS
                     LSWSGDGAWLATSGAEAAIIWPFSGTGPTGKAPRECGVRPKRTTQVAFHPKALVLAIG
                     YEDGWVLLVRLTDAAELMVRRGTGGPVTALAWDGAGQRLLFGTEDGAAGLLTLPGG"
     gene            complement(3237..4265)
                     /locus_tag="PK812_04725"
     CDS             complement(3237..4265)
                     /locus_tag="PK812_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRJ68893.1"
                     /translation="MSDKIPVTVLTGYLGAGKTTLLNRILSENHGKRYAVIVNEFGEI
                     GIDNEIVVGADEEVFEMNNGCICCTVRGDLIRIVEGLMRRKGRFDAIIIETTGLADPA
                     PVAQTFFVDQDVQDRARLDAIVTVADAKWLSARLKDAPEAKNQIAFADVILLNKTDLV
                     TPAELEAVKGRIRAINPYARMITTQKCNAPLDEVLDRHAFDLERILEIEPAFLDDGHD
                     HHHDHDHGDDHGHHHHHHGGLKHYHDEEMQSVAIRIDGDVDPDKFMPWINDLVQRRGP
                     DILRSKGILAFKNEPKRFVYQGVHMILDGDLQRDWRPDEPRVSTIVFIGRHLDEKELR
                     EGFTACAA"
     gene            complement(4288..5217)
                     /locus_tag="PK812_04730"
     CDS             complement(4288..5217)
                     /locus_tag="PK812_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HRJ68894.1"
                     /translation="MIHRRNVLTSILAVAFVGGAAAQTPAPRIPVTASFSIVADLVRE
                     VGGDRIEVSALVGPGQDAHVYQPTPADARTISQAKGIFINGLHFEEFMGRLIKTAGAK
                     APVFRAAAGFEPLKASGKHSHGDHKGHSHGDFAPDPHAWHSVPNVKAYVGNISKGLAQ
                     IDPDGATVYAQRAAAYIEKLDALDREIRAAVEDIPKERRIAVTTHDAFAYLAREYGIQ
                     FVTLQGVSTDTEISAAGLGKTIREVKARKAPAVFIENITDPRKIERLSKETGAKLGGT
                     LYSDALSKADGPAPTYIELMRHNIRQLKQALAP"
     gene            complement(5253..6125)
                     /locus_tag="PK812_04735"
     CDS             complement(5253..6125)
                     /locus_tag="PK812_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HRJ68895.1"
                     /translation="MEGIASLLAPFAEFGFMRRALAGCVALSVSAPLIGVFLMLRRMS
                     LVGDAIQHAILPGAALGYLVAGLSLGAMTLGGLVAGLAVALLAGLITRTTNLREDTAI
                     ATFYILSLALGVTLLTARGTNVDVLRVLFGSVLALDDATLLLMTGVSSITVLALALFL
                     RPMVLEFADSGFLRSVSAAGGAMHFLFLGLAVLNMVAAFHALGTLLSIGIMILPAATA
                     RLWTNDLTRLMASSVAIALASCSVGLVASYHLELASGPAIILVAGCVYMASILAGRAG
                     GLLPRLIPRRHLEG"
     gene            complement(6125..6865)
                     /gene="aztA"
                     /locus_tag="PK812_04740"
     CDS             complement(6125..6865)
                     /gene="aztA"
                     /locus_tag="PK812_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein AztA"
                     /protein_id="HRJ68896.1"
                     /translation="MNTAIAFENLTLGYDRHPAVHHLNGEIARGALLAVVGPNGAGKS
                     TLLKSIAGILRPLDGAIRVAGAESGSLAYLPQIIESDRSFPISVHEFIAMGAWRQIGM
                     FAGLSRAVRSRIDETVAAVGLAGFASRPIGTLSGGQMQRALFARLMLQDASTILLDEP
                     FASIDQKTTQDLLDIVRHWHAEGRTVLAVLHDLALVRAAFPRTLLLARELIAWGPTEQ
                     ALSPANLMAARNMVAAFDSDAHACERAA"
     gene            complement(6865..7092)
                     /locus_tag="PK812_04745"
     CDS             complement(6865..7092)
                     /locus_tag="PK812_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68897.1"
                     /translation="MHTHTDGPRKHVHDHGHGHAHGHSAVGTGEAQRHAHAVVDELPP
                     ASILTSSLSVRLSLAGAVVIILWCVTWWALG"
     gene            complement(7199..8758)
                     /locus_tag="PK812_04750"
     CDS             complement(7199..8758)
                     /locus_tag="PK812_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein D"
                     /protein_id="HRJ68898.1"
                     /translation="MDRRSFVTGLAATGLPLSQALAQTPGLDLGPEASFSFETLVDRA
                     RRAAGGAYVEPPRPAPDVLQKIDYDAHGKIRFKTDLAVWGRAPDQRRHPVTFFHLGRY
                     FQKPVRMHVVENGKAREIIYREDYFDMPAESPAKALPKGAGFAGFRFQEATDGALDWR
                     KNDWVAFLGASYFRAIGELFQYGLSARGLMIDAAVSTPEEFPDFTHVWLEQPKGGDGV
                     RAYALLDSPSAAGAFRFDMRRGKAVVMDVEKRLFVRKPVTRFGIAPLTSMYWFSEKAK
                     GTAVDWRPEVHDSDGLALWTGSGERIWRPLNNPPRTVASSFGDTNPKGFGLLQRDRAF
                     DHYQDGVYYEKRPSLWVEPLGGWGKGAVQLIEIPTDDEIHDNIVAAWIPDQPVKSGDQ
                     LALRYRLHWVADEPYPTPLARVVATRLGNGGQPGQPRPKGVRKFLVEFKGTPLEKLPS
                     GVKPEAVLWASRGSYSYVFTEAVPNDVPGHWRAQFDLTAEGADPVEMRLFLRLDGQPL
                     SETWLYQYHPF"
     gene            complement(9005..9235)
                     /locus_tag="PK812_04755"
     CDS             complement(9005..9235)
                     /locus_tag="PK812_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68899.1"
                     /translation="MAKKLNRQNLITMTSVAVLVGTEILAASLATGWAIGGLMQLGRE
                     VTWGIIAVSLAGGLWATIKFVQNALKVEPIYE"
     gene            9462..10082
                     /locus_tag="PK812_04760"
     CDS             9462..10082
                     /locus_tag="PK812_04760"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRJ68900.1"
                     /translation="MRNGFFSSALTAAVVMAGCALAPAQAFQPVGSYLTMKQTPVMTI
                     QPAAFPTTLPIQRNEDRHDRATVAMPGTYEAGAILVVNSQRKLYLSLGDGRALVYPIA
                     IGKPGMSWTGETRVTGKAKNPTWTPTANTRRLKPGLPAFVAAGPKNPLGPRAVYLAKG
                     YYRIHGTNQPSSIGSAASLGCFRMYNSDVVDLFSRVETGARVVVIN"
     gene            complement(10193..11143)
                     /locus_tag="PK812_04765"
     CDS             complement(10193..11143)
                     /locus_tag="PK812_04765"
                     /inference="COORDINATES: protein motif:HMM:NF015763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel transporter"
                     /protein_id="HRJ68901.1"
                     /translation="MLTAPDLLAQTTGKRPFGVGDAIPANAEPAGGITAWLLAKQAEY
                     TRAMIAALRATREGAGAWALITVAFAYGVFHAAGPGHGKAVVMSYVFANEQVIRRAVG
                     IAVAAAILQALVAIALVLPAVLLVGATARQLDTGVYWIEIASFSAIALLGLSLIVRKA
                     RALRQALADRRMPAPVGGSIPACSDSDPDHVHGPHCAHVHLPQASELTGKMSWQELAA
                     ATLAAGSRPCSGAIILLAFALSVGATAVGIAAVFAMAAGTALTTAGFAVGAVAAKGLA
                     TRLADRGERLGLVTAILELVAAVLVALLGLALLLGYVAAG"
     gene            complement(11218..11877)
                     /locus_tag="PK812_04770"
     CDS             complement(11218..11877)
                     /locus_tag="PK812_04770"
                     /inference="COORDINATES: protein motif:HMM:NF017985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1007 family protein"
                     /protein_id="HRJ68902.1"
                     /translation="MTIRSGTQPLLRAFLTAAAIAVAGAAHAHPHVLVTAKSDFMLDG
                     SGKITGVRHAWTFDEAYSAYAVTGLKAGKDGKVPRENLVDLAKLNVESLQDFSYFTAL
                     KQGKKAFPFNNPADGYYLEHDGKALILHFVLPLKEPVAAERTTTLEVQDESFFVAFSF
                     AEKDPIGIEGPANSCRVEMKHPKKLLPSGDVGRISEDFFNSIRPGSLDQFFSSARLVC
                     P"
     gene            12092..14176
                     /locus_tag="PK812_04775"
                     /pseudo
     CDS             12092..14176
                     /locus_tag="PK812_04775"
                     /inference="COORDINATES: protein motif:HMM:NF009589.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
BASE COUNT         2591 a         4591 c         4648 g         2383 t
ORIGIN      
        1 gtccgcaacc gctgccgcag ccgctatccg gcgcgaccgg ggccggcggc gaaacggagg
       61 cggaccgcgc ctgataaggc cgggcttggc aacctttcca ttgacggacg cggccggttt
      121 ccgcatgaac cggccgcgct gcatcgagct tctgcgggag acggaaatga acgactggcc
      181 tgtccacggc acaatcaccg gtccgattgt gatgatcggt ttcggttcta tcggcaaggg
      241 cacgctgccg ctgatcgagc ggcatttccg ttacgaccgc gcgcgcctgg tggtgatcga
      301 tcctgaggac aaggatcgcc ccttgctcga tgaacttggc gtgcgcttca tccaccgcgc
      361 cgtcacgcgc gacaactacc gggagctgct caagccgctg ttgacggaag gcggtggcca
      421 gggtttctgc gtcaatctct cggtggacac ctcatcggtc gacatcatga cgttctgcca
      481 ggagatcggc gccctctaca tcgacacggt gaacgagccc tgggcgggct tctacttcaa
      541 cctgagcgcc ggtccggccg cgcgctcgaa ctacgcgctt cgcgagacga ctctcgccgc
      601 cgcgcgctcg acccgtggca agccgaccgc agtttcctgt tgcggtgcca atccgggcat
      661 ggtgtcttgg tttgtcaagc aggcgctgct caatgtcgcg accgacctcg gcctgagctt
      721 cgaggaaccg aagacgcgcg aggactgggc gcgcctgatg caggcctgcg gcgtcaaggg
      781 cgtgcatatc gcggagcgcg atacccagcg atcgaggaat ccgaagcctc tcaacgtgtt
      841 cgtgaacacc tggtcggtcg agggcttcgt ttcggaaggc atgcagccgg cggagctcgg
      901 ctggggcacg cacgaggcct ggatgccgga gaatggacgg acgcacgaga ccgggtgcgg
      961 cgcagcgatc tacctgctcc agccgggggc gaatacccgg gtccgctctt ggtgcccgac
     1021 gccaggcgcg cagtacggat tcctcgtcac ccacaacgag tcgatctcga tcgccgacta
     1081 tttcaccgtc cgcgatgcat cgggcaaggc cgtctaccgg ccgacctgcc actatgccta
     1141 ccacccggcc aatgacgccg tcctctccct gcatgagatg ttcggcgccg agggccggcg
     1201 ccagccgaca tggcacatcc tcgacgagaa cgagatcgtg gacggcatcg atgaactcgg
     1261 tgtgctgctc tatggccacg gcaagaatgc ctactggtac ggctcccagc tttccatcga
     1321 ggagacgcgc cgcatcgccc cgtaccagaa cgcgaccggc ctgcaggtga cctcggctgt
     1381 gctggcgggc atggtctggg cgctggaaaa cccgaatgcc ggaatcgtcg aggcggacga
     1441 gatggatttc aggcgctgcc ttgaggtgca gctgccctat ctcggtccgg tcatcggcgc
     1501 ctataccgac tggacgccgc tcgacaaccg gggaaagctg ttctcggagg atgtggacac
     1561 cgccgacccc tggcagtttc gcaacatcct ggtgcgctga ccatccggcg ggccgcgagg
     1621 ggcgaagcct cgcgtcgtgc gctcgctcgc ggcgtcaggc gtagtgcgtc tccttgcgcg
     1681 cctgcagcct gcgggcgatc gcccgttttg gccagccgag cagccgcgcg aggatccagc
     1741 cgacaaagcc gccgagaatc gccagaacgc cgccttctcc ggtgaaaggt atggccggct
     1801 cgtagtcatt gaaagtcgag cgggcggttt cccggtcgag ccgcgtcgcg agagccatcg
     1861 tcaggttgcc acccgacagc ttcgccatgt cgtcgcggaa gcgctccagc cgctgctggc
     1921 gtgcgatggt gtcaatcatc gccgcgccgc gtttgcggaa cagttcctcg gcgttctcgg
     1981 tgtgcttcct cagagcctcg gcgcgcgaca gaccttcgga gcgggcatcc gcatcgaagc
     2041 gttcgacgat cgcgcgcagc tcatcggtgg cgccgccaag gcgctggcga tactgctgcg
     2101 cgaattccgg aagctggacc gcactgacgc cgcccaccac caccattgcg cttgtcagta
     2161 gcctggcgat cacgccgcac ctccgtgctg gcttcgatgc tccaccgtcc ccgccggcag
     2221 gatcgatcaa ccgccaggca aggtcaacag tccggcagcg ccatcctcgg tgccgaacag
     2281 caagcgctgc ccggcgccat cccaggccag ggcggtgaca gggccgccgg ttccacgccg
     2341 caccatgagt tccgccgcat cggtcaggcg cacgagcagc acccagccat cctcgtagcc
     2401 gatggccaga accagcgcct tggggtggaa cgcgacctgc gtcgtccgtt tcggcctgac
     2461 gccgcactcg cgcggcgcct ttccggtggg acccgtcccg gagaatggcc agatgatcgc
     2521 ggcctcggcg ccagaggtcg ccagccacgc gccatcgccg gaccatgaca gggaacgcgt
     2581 cttggccggg tagcccgaca tgcgcatatg cgtcttctca ggcagcttcc agccgtgcag
     2641 ctggttctcc tgcatggaag agaccacaaa tcgtccatcc ggtgaccagg tcacgtcgag
     2701 atgcgaccct ttccattcca gtctctctgg ctcggcggag accgccggaa accacagggt
     2761 tgtgccattg gcatgcgttg cggcgagccg gtatcccttc ggcgcaaagc acaagccacg
     2821 ggaggacgag ggcaggtcga gcgacttgac ccgtcctttg ccgtcgcggg cgttgacgcg
     2881 tttgccgaca gaccaggcgg tcgcgccgga agcgccgagg gcgacagcat cgatccagcg
     2941 gcccttttca tccgccagcg tggcaagcga cccactggcg tcgatggcga tgaccttgcc
     3001 gtcatcgccg ccggtcacga ggcgcgtcgc atcgtctgcc gcgacgagaa tggttgcgtc
     3061 atgcgcctgc acctcgcgcg cccccgaggc atcgccgatg cggatgcggc catcgctcat
     3121 ggccagaacg gggacctttc ccagaaaggc agcatgggcg acgaaatccc cggctggaag
     3181 gtcggcgacg tgttgcgtca aggtctcaaa cgatgcagcg gtcatctggc ttcgcctcag
     3241 gcggcgcagg ccgtgaagcc ttcgcgcagc tccttttcgt cgagatggcg accgatgaac
     3301 acgatcgtgg agacgcgcgg ttcatccggt cgccagtcgc gctgaagatc gccatcgagg
     3361 atcatgtgca cgccttggta gacgaaccgc ttcggctcat tcttgaaggc caggatgccc
     3421 ttcgagcgca ggatgtcggg gccgcggcgc tgcaccagat cgttgatcca gggcatgaac
     3481 ttgtcgggat cgacatcgcc gtcgatgcgg atcgccacgg attgcatctc ctcgtcgtga
     3541 tagtgcttca gcccgccgtg gtgatggtgg tggtggccgt gatcgtcgcc gtgatcgtga
     3601 tcgtggtgat ggtcatggcc gtcgtccagg aaggcgggct cgatctccag gatgcgctcc
     3661 agatcgaaag catgccgatc gaggacctcg tccagcgggg cattgcactt ctgggtggtg
     3721 atcatgcggg cgtaggggtt gatggcgcgg atgcggccct tcaccgcctc cagttccgcc
     3781 ggcgtcacca gatcggtctt gttgagcagg atcacgtcgg cgaaggcgat ctggttcttg
     3841 gcttccggcg cgtccttcag ccgggccgag agccacttgg cgtccgccac ggtgacgatc
     3901 gcatccaggc gtgcgcgatc ctgcacgtcc tggtcgacga agaaggtctg cgcgacaggc
     3961 gcgggatcgg caaggccggt ggtttcgatg atgatggcgt cgaaccggcc cttgcggcgc
     4021 atcaatccct cgacgatgcg gatcaggtcg ccgcgcacgg tgcagcagat gcagccgttg
     4081 ttcatctcga aaacttcctc gtcggcgccg acgacgatct cgttgtcgat gccgatttcg
     4141 ccgaactcgt tgacgatcac ggcatagcgc ttgccgtggt tctccgagag gatgcggttg
     4201 agcagcgtcg tcttgccggc accgaggtag ccggtgagca cggtgacggg gatcttgtcg
     4261 gacatttcaa ctccagcgta gcgatcgtca gggggcgaga gcctgtttca gctgccttat
     4321 attgtgtcgc atcagctcaa tgtaagtggg ggccggccca tcggccttcg agagcgcgtc
     4381 ggaatagagc gtgccaccca gtttggcccc ggtctccttg gagagccgct cgatcttgcg
     4441 tggatcggtg atgttctcga tgaagacagc cggcgctttc ctggccttca cctcgcggat
     4501 ggtcttgcca aggccggctg ccgagatctc cgtgtcggtc gacacgccct gcagggtaac
     4561 gaactggatg ccgtattccc tggcgaggta agcgaaagcg tcgtgcgtcg tcaccgcgat
     4621 acggcgctcc ttcggaatgt cctcgacggc ggcgcggatt tcccggtcga gggcgtcgag
     4681 cttctcgata taggccgccg cgcgctgcgc atagaccgtt gcaccgtccg ggtcgatctg
     4741 ggcaagtccc ttggagatgt tcccgacata ggccttcacg ttcgggacgg aatgccaggc
     4801 gtgcggatcg ggcgcgaagt cgccgtgcga gtgccccttg tgatcgccgt gactgtgttt
     4861 gccagatgcc ttgagcggct cgaaacccgc agccgcgcga aagacgggag ccttggcgcc
     4921 ggcggtcttg atcagccggc ccatgaactc ttcgaagtgc aggccgttga tgaagatgcc
     4981 cttggcctgg ctgatcgtgc gggcgtcagc cggagtcggc tgatagacat gcgcgtcctg
     5041 accagggccg accagcgccg atacctcgat gcggtcacca ccgacctcgc gcaccaggtc
     5101 cgcgacaatg gagaagctcg cggtgaccgg aatgcgtggc gcgggcgtct gcgccgccgc
     5161 gccgccaacg aaagcgaccg ccagaatgga tgttagaaca ttacggcgat ggatcatggt
     5221 atcctccctt ggtggatgca tggcgcggtc tgtcagcctt cgagatggcg gcgcgggatg
     5281 aggcggggca agaggccgcc agcgcggccg gcaaggatcg aggccatgta cacgcagccc
     5341 gccacgagaa tgatcgccgg gccgctggcc agttcgagat ggtaggaggc gacaagcccg
     5401 acggaacagg aggccagcgc gatcgccacc gagcttgcca tcaggcgtgt caggtcgttg
     5461 gtccagagcc gcgcggtcgc ggccggcagg atcatgatgc caatggacag aagcgtgccg
     5521 agcgcatgga aggccgccac catgttcagc acggccagcc ccaggaacag gaagtgcatc
     5581 gcgccgccgg cggcactgac cgagcgcagg aagccgctgt cggcgaattc cagcaccatc
     5641 ggccgcagga acagggccag cgccagaacc gtgatgctcg agacgccggt catgagcaga
     5701 agcgtggcgt catccagcgc gagcacggac ccgaacaaca cgcgcagcac atcgacattg
     5761 gtgccgcgcg ccgtgagcag cgtcacgccc agtgcaagcg acaggatgta gaaggtcgcg
     5821 atggccgtat cctcgcgcag attggtggtg cgggtgatga ggcccgccag cagggcaacc
     5881 gccagcccgg ccacaaggcc cccgagcgtc atcgcgccga gcgacagccc ggcaacgaga
     5941 tagccgagcg ccgcgccggg caggatcgca tgctggatcg catcgccgac gaggctcatg
     6001 cggcgcaaca tcaggaacac gccgatcagc ggcgcgctga ccgacagggc gacgcagccg
     6061 gcgagcgcgc ggcgcatgaa gccgaactcg gcgaaaggcg ccagcaggct tgcgatccct
     6121 tccatcaggc ggcccgttcg caggcgtggg cgtcgctgtc gaaggctgcc accatgttcc
     6181 gcgcggccat gaggttcgcc ggggaaaggg cctgctctgt gggcccccag gcaatgagtt
     6241 cgcgcgccag cagcagcgtt cgcggaaagg ccgcacgcac cagcgcgagg tcatgcagca
     6301 ccgccaggac ggtccgaccc tcggcatgcc agtgccgcac gatgtccaag aggtcctgcg
     6361 tggtcttctg gtcgatcgag gcaaagggct cgtccagcag gatggtggaa gcatcctgca
     6421 gcatcagtcg ggcgaacagc gcacgctgca tctgcccgcc ggacagcgtt ccgatcggtc
     6481 gggatgcaaa cccggcaagg ccgactgccg ccaccgtttc atcgattcgg gaccgcaccg
     6541 cacgggagag gccggcgaac atgccgatct ggcgccaggc gcccatggca atgaactcgt
     6601 gcacgctgat cggaaagctg cggtcgcttt cgatgatctg gggcaggtag gcgaggcttc
     6661 ccgactcggc accggcaacg cggatcgcgc catccagcgg cctcaggata ccggcgatgc
     6721 tcttgagcag cgtggatttc ccggcgccgt tcgggccgac gaccgccagc agggctcccc
     6781 gcgcgatctc cccgttcaga tgatgcaccg ccgggtgccg atcatagccg agcgtcaggt
     6841 tctcgaaggc gatcgcggtg ttcatcagcc caacgcccac caggtcacac accagagtat
     6901 gatcaccacc gcacccgcca aggaaagtcg taccgagagg ctggacgtga ggatcgaggc
     6961 cggcggcagc tcatccacaa cggcgtgggc atgacgctgc gcctcgccgg tcccgaccgc
     7021 gctgtgtccg tgcgcatggc catgcccgtg atcgtggaca tgcttgcgcg ggccatcggt
     7081 gtgcgtgtgc atgcgattgt aatattataa cacagtgtca ggcgtcaact ttggcctgga
     7141 ggctgcttcg aacgagccaa ccaggtggcg gagtttctgg cgcgtgtggg cgccttattc
     7201 agaacggatg gtactggtag agccaggttt cactcagggg ctgtccatcg agccgcagaa
     7261 acagccgcat ctcgaccggg tcggcgcctt ccgcggtcag gtcgaattgc gcgcgccagt
     7321 ggcctggaac gtcgttgggg acggcttccg tgaagacata ggaataagaa ccgcgcgagg
     7381 cccagagtac ggcctcgggc ttcacgccac ttggcagttt ctccagcggc gtccccttga
     7441 actcgacgag gaacttgcgc acgcccttcg ggcggggctg ccccggctgt cccccattgc
     7501 ccagccgcgt cgccaccacg cgggccagcg gcgtgggata gggttcatcg gcgacccagt
     7561 gcagcctgta cctgagcgcg agctgatctc cagacttgac cggttggtct ggaatccatg
     7621 ccgcgacaat attgtcgtga atttcgtcat cggtcgggat ttcgatgagc tgcacagcgc
     7681 ccttgcccca tccgccgagc ggctcaaccc acaggcttgg gcgcttttcg taatagacgc
     7741 cgtcctggta gtgatcgaag gcacggtcgc gctggagcag accgaagcct ttcgggttgg
     7801 tgtccccgaa ggacgaggcg accgtgcgcg gcgggttgtt cagggggcgc cagatgcgct
     7861 cgcccgagcc agtccaaagc gcgaggccat cggaatcgtg aacttccggc cgccagtcca
     7921 cggcggtccc cttcgccttc tcggagaacc agtacatcga ggtgagcggg gcgatgccga
     7981 agcgggtaac gggcttgcgg acgaacagcc gcttctcgac atccatgacc acggccttgc
     8041 cgcgtcgcat gtcgaaccgg aaggctccgg cggcgctcgg gctgtcaagg agcgcatacg
     8101 ccctgacgcc atcgccgcct ttcggctgct ccagccagac atgggtgaaa tcaggaaact
     8161 cctcgggcgt gctgacggcc gcatcgatca tcagacctcg cgccgacagc ccgtactgga
     8221 acaattcgcc aatggcgcgg aaataggatg cgccaaggaa ggctacccag tcgttctttc
     8281 tccagtcgag ggcgccgtcg gtcgcttcct ggaagcggaa gccggcaaag cccgcaccct
     8341 tgggcagggc cttggcgggc gactccgcag gcatgtcgaa gtagtcctcg cgatagatga
     8401 tctctcgggc cttgccgttt tcgaccacgt gcatccgcac cggcttctgg aaataccgac
     8461 cgagatggaa gaacgtcacg ggatggcggc gctgatccgg cgcgcggccc cagacagcga
     8521 ggtcggtctt gaagcggatc ttgccgtggg cgtcgtagtc gatcttctgc agcacgtcag
     8581 gcgccgggcg cggcggctcc acataggccc cgcccgccgc acggcgcgca cggtcgacga
     8641 gggtctcgaa ggagaacgat gcttccgggc ccagatccag ccccggggtc tgggcgagcg
     8701 cctgcgacaa cggcagtccg gtggccgcga ggccggtgac aaaggaacgg cgatccatgt
     8761 tcggcagaac tccacgatac cggcgcgata taggtttcca caccgctgct gtcgaccggc
     8821 gattggcccg ctggcgtgca atgccacggc agcagacggc gaaggagtac caggtttcag
     8881 gcccgccatg gcgggctgcg caaacgcggc gaccggttcc tgggccgtgg ccgcgacggg
     8941 agcatacttc agagcggatg cagtcctgtc gatgaagcgg atgtcctctc ccgccccggc
     9001 ggggttactc gtagatcggc tcgaccttca aagcgttttg cacgaacttg atggtcgccc
     9061 acaggccgcc ggcgaggctc acggcaatga tgccccaggt cacttcccgg ccaagttgca
     9121 tcaagccgcc aatggcccaa cccgtcgcca gcgaggcggc aaggatctcc gtgccgacaa
     9181 ggactgcgac gcttgtcatg gtaatgaggt tctggcgatt cagcttcttg gccatgcccg
     9241 gctccgtcgt tcgtggcagc gctagcccaa atgcggcttg cgcgcaacgg cgcggtgcgc
     9301 gccgggcgct cccccgccct cgccgagagg ttaacaaacc cggttaagtt ttccacgatg
     9361 ccatgtgccg aagcgcacag cggcacgagg gaaggcccgg catagtcacg ctcacgaaaa
     9421 cggcgcccac gagatgcgcc gcaccccaat cggaggcgac tatgcgtaac ggtttctttt
     9481 cttccgccct gactgccgcc gtcgtgatgg cgggctgcgc gctggctccg gctcaggcgt
     9541 tccagccggt cggttcctat ctcacgatga agcagacgcc ggtcatgacg atccagcccg
     9601 cggctttccc gacgacgctg ccgatccagc gcaacgaaga ccgtcatgac cgcgccaccg
     9661 tggcgatgcc gggcacctac gaggcgggcg cgatcctggt cgtcaactcg cagcgcaagc
     9721 tctacctgtc gctcggcgat ggccgcgccc tggtctatcc gatcgcaatc ggcaagcccg
     9781 gcatgtcctg gaccggcgag acccgcgtca ccggcaaggc caagaacccg acctggaccc
     9841 cgaccgccaa cacccgccgg ctgaagcccg gcctgccggc ctttgtcgcc gccggcccga
     9901 agaatccgct cggcccgcgc gccgtctatc tcgcgaaggg ctattaccgc atccacggca
     9961 ccaaccagcc gtcctccatc ggcagcgccg cttcgctcgg ctgcttccgg atgtacaaca
    10021 gcgatgtggt ggacctgttc tcgcgggttg agaccggcgc ccgggtggtt gtcatcaact
    10081 gacccggagc catcgtcaga ccgggggtcg tgcaaggcgg cttccggttt gaccacgaag
    10141 cgtcctaacc cgcgaaaatc acgggaacat ttccgcttct tgcaatccgg cttcagccgg
    10201 cggcgacata gccaagcagc agcgcaagtc caagcagggc gacaaggacc gccgcgacca
    10261 gttcgaggat cgcggtgacc agccccaggc gctctccccg atccgccagt cgcgtcgcca
    10321 atcccttggc cgccacggcg cccacggcaa aacccgccgt ggtcagggcg gttccggccg
    10381 ccatggcaaa tacggcggcg atcccgacag ccgtcgcgcc gacggagagc gcaaaggcca
    10441 gcaggatgat ggcgcccgag cagggccgcg atccggcggc gagggtcgca gcagccagct
    10501 cctgccagga cattttccct gtcaattcag aagcttgtgg cagatgaacg tgggcgcaat
    10561 gcggtccgtg cacatgatcc ggatcgctgt cggaacaggc ggggatgctc ccgccgacgg
    10621 gtgcgggcat acgccggtcc gcaagggcct gacgcagggc ccgggccttg cgcacgatca
    10681 gcgacaggcc tagcagggcg atggcgctga agctggcgat ctcgatccag tagacgccgg
    10741 tgtcgagttg gcgagccgtc gcgccaacca gcagcaccgc cggcaggacc agcgctatgg
    10801 ccaccagagc ctgcaggatg gcggcggcaa cagcaatgcc gacggcgcga cgtatgacct
    10861 gctcgttggc aaaaacataa gacattacaa cggctttccc atggcccggt ccggcggcat
    10921 ggaacacgcc gtaggcaaag gcgacggtga tcagcgccca ggctccagcg ccttcccggg
    10981 tggccctgag ggcggcgatc atggcccggg tgtattccgc ctgcttggcg agcaaccagg
    11041 cagtgatgcc tccagccggc tccgcattgg cgggaattgc gtcgccgacg ccgaacgggc
    11101 gcttcccggt ggtctgggcc agaagatcgg gggcggtcag catcacggcg cataaagcga
    11161 gaataatcac ggccgccagg tgccagactg catcgtattc ctgttttata cggtatttca
    11221 cgggcagacg aggcgcgcag agctgaagaa ctggtccaaa gaccctggcc tgatactatt
    11281 gaagaagtcc tcagaaatcc tgccgacgtc gccggatgga agtaacttct tgggatgttt
    11341 catttccacg cggcaggaat ttgccggtcc ttcgatgccg atagggtcct tctcggcaaa
    11401 agaaaacgca acgaaaaagc tttcatcctg cacttcaagc gttgtcgtcc gctcggcggc
    11461 gacaggctct ttcaagggca ggacaaagtg caggattagt gcctttccgt cgtgttcaag
    11521 gtaatatcca tcggcggggt tattaaaagg aaaagccttc tttccctgct tcaatgcggt
    11581 gaaatacgaa aagtcctgca aggactcgac gttcaatttg gcaaggtcga ccagattttc
    11641 tcgcggaacc ttgccatcct tgccggcttt caggcccgtg accgcatagg cggaataggc
    11701 ctcgtcgaag gtccaggcat ggcggacgcc ggtgatcttg ccagacccgt caagcatgaa
    11761 atcggatttt gccgtcacca gcacatgagg atgcgcatgc gccgcgcccg cgaccgcaat
    11821 ggcggctgcg gtcagaaatg ctcgtagaag tggttgtgtt ccagagcgaa tcgtcatgtt
    11881 cgcggcactc ctcgcaccaa aacaagatga tgttcggaca gggattcggc aagggtcacg
    11941 gatcggatca atccgcggca ttttggtggc gttacgcaga gcatccgcgc aaatgtgaac
    12001 gtcaccccct agaaacgtca gcaatgctga catatacgaa ggttcaagag aatcagacag
    12061 agtggcgtcc gccgccggag gggagttgag catgcacgcg tccgcagccc tgcgcgaagt
    12121 cagcctcgac gacaagttcg atctttccca ggaacaggtg ttcatgtccg gcacgcaggc
    12181 gatcgtccgc ctgctgctga tgcagcgcga gcgcgaccgg ctcgcggggt tgaatacggc
    12241 ggggttcgtt tcaggctacc gcggctcgcc catcggcggg ctggatcagc agctgttcag
    12301 ggtacgccgg cagctcgaga aggcgaacat cgtgttccag cccggcctca acgaggacct
    12361 cgcggcgacg gcgtgctggg gtgcgcagca ggcggaactg aggggcgaag gcaagtacga
    12421 cggcgtcttc acgctgtggt acggcaaggg gccgggcgtc gaccgcaccg gcgacgtgtt
    12481 ccgccacgcc aacatggccg gcacctcggc gcatggcggc tgcatcgcgc tggccggcga
    12541 cgaccacacg gccgaatcct cgaccaacgc gcatcagacg gaattcaatt tcgtcgacta
    12601 catgatgccg atcctcaatc cggccggcgt gcaggagatc ctcgactacg gactgcacgc
    12661 ctatgcgatg agccgcttcg cctcggtctg ggtggcgctc aaggcagtga aggacaacat
    12721 cgaatccacc gcttccgtcg acgggtcgct gcatcgcgtg aagatcgtgc ggccgacaaa
    12781 tttcgccatg ccgccgggcg gcatcaacat ccgccgcatg gacaacttcc tcgagcagga
    12841 agagcggctg catgaggtca agcgatcggc ggccgttgcc tatgtcagcg ccaacagcct
    12901 gaaccagacg atctattccg gtgggccgaa tgcgcgcatc ggcatcatca cggtgggcaa
    12961 gagctatctc gacacgcggc aggcgttcgg cgagctcggc attgacgaga agcgggcgag
    13021 cgacctcggc atccgcctgt tcaaggtggc ctgcacctgg ccgatcgtgc cgcaggaaat
    13081 cctcgacttc gcgcgcggcc tcgagaccgt gatcgtggtg gaggaaaagc gcgcgctgat
    13141 cgaggtgcag gtgcgcgagg agctctacgg catcgccgac cagccgcgcc tcatcggcaa
    13201 gaaggacgag aatggcaact ggctgttccc ggtcaagaac gctctcgacc ccaacgagat
    13261 cgccatcgcg ctcggcgagc gcatcctcga ataccgcaag gacgagagca tccgcgccaa
    13321 cgtcacccgg ctgaaagagg cgcagaacgc gctcaagacc atcaccgacg cggcggtgcg
    13381 catcccctat ttctgctcgg gctgcccgca taattcctcc acggtcattc ccgagggcgc
    13441 gcgcgccttc gcgggcatcg gctgccactt catggcgatg tggatggatc gcggcaccga
    13501 aggcttcacg cagatggggg gcgagggcgc caactgggtc ggcgaagggc cgttctcgcg
    13561 ccgcaagcat gtgttccaga atctcggcga tggaacctac aaccactccg gctcgctggc
    13621 cctgcgcttc gccgtcgctt cgaaggcgac catcacctac aagatcctgt tcaatgacgc
    13681 cgtggccatg accggcgggc agatgcacga aggcgcgctg acgcccgacg ccattgcccg
    13741 gcaggttcgc gccgaagggg tcgagcgcat cgccatcgtc tcggacgagc cggaaaaata
    13801 cgaccaccgc taccatttcc cgccgggcgc gacgttccac caccgttcgg aaatcatccc
    13861 ggtgcagcag gaactggcga cggtcgaggg cgtctccgtg ctgatctacg accagacctg
    13921 cgcggcggag aagcgccggc ggcgcaagcg cggcaccttc cccgatccgg acaagcgcgt
    13981 gatcatcaac gaactggtgt gcgagggctg tggcgactgc ggcaagaagt cgaactgcgt
    14041 ctcggtgcag ccgctggaga ccgaatacgg ccgcaagcgc cgcatcgacc agtcgaactg
    14101 caacaaggat ttttcctgcc tcgagggctt ctgtccctcc ttcgtcacca tcgagggcgc
    14161 gcggctgaag aaggcggcgc caaaggccgg ggccgcgcgc cctcgatggt gac
//
LOCUS       DAOMXN010000035        14151 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_2358_length_14151_cov_11.016175, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000035 DAOMXN010000000
VERSION     DAOMXN010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 14151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14151
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2358_length_14151_cov_11.016175"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..707)
                     /locus_tag="PK812_04780"
     CDS             complement(117..707)
                     /locus_tag="PK812_04780"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HRJ68903.1"
                     /translation="MREVEAKSLKQKIALGLLHAFGWKVIARDVPDKCVIIGYSHTSN
                     WDFLFFVLARMAYGMNLSYLAKAEIFRFGAGPVLKALGGIPVDRSAAQGVVGEAVAMF
                     GRMPELRLAISPEGTRKRTEYFKSGFYHIAIGAGVPLLLAALDFGTRTIDIGPAIALT
                     GDAKADMNAIRARYEGVRGYHPEKMGVLRMRDEDAG"
     gene            complement(710..>1090)
                     /locus_tag="PK812_04785"
     CDS             complement(710..>1090)
                     /locus_tag="PK812_04785"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HRJ68904.1"
                     /translation="VGGHVDGVCDTITSISGAVEGKQVKALAVASPVRLGKHPEVPTA
                     AEAGLPEFQQEGWNAIFAPAGTPQPVIDRLNAALRKAVASPLVQGRLKELSALPASEQ
                     EMTPAHVTALVPKEVDKYRALLKD"
     assembly_gap    1091..1190
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1191..1507)
                     /locus_tag="PK812_04790"
     CDS             complement(<1191..1507)
                     /locus_tag="PK812_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HRJ68905.1"
                     /translation="MVRLAALALFAAALPAFAQDAYPSRPITLIVPFAPGGTSDVIGR
                     LAAEEMGNVLGQRILIENLAGAGGSTAMARAAKAAPDGYTLIVGNAGTAGAVYSLYPN
                     LTF"
     gene            complement(1575..2228)
                     /locus_tag="PK812_04795"
     CDS             complement(1575..2228)
                     /locus_tag="PK812_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRJ68906.1"
                     /translation="MSLTLYIGNKAYSSWSLRPWVAMTHMGIRFDEVVIPLDLPDTRE
                     KILAASPSGRVPALRDGAILIWESLAILDYLDEFHVRGRLWPLDRAARATARSIACEM
                     HSGFSALRTHCPHNCRRDPAKPFALPAAARSDVDRIEAIWRDTRGRFGQSGQFLFGTF
                     SAADAMYAPVVNRFHHYGIAVADDTRAYMDSVLNLPAMQAWKKAAEAEPWVIAASEL"
     gene            complement(2242..2688)
                     /locus_tag="PK812_04800"
     CDS             complement(2242..2688)
                     /locus_tag="PK812_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRJ68907.1"
                     /translation="MAEVTIRRPLAGDETAWRALWAGYIRFYRGSVSAEATAALWAEI
                     LVERGKFEAFVAERAGETIGFVHYLYHASTWSTQPICYLQDLYVDPHARGGGAARALM
                     DAVFDAAKADGAFRVYWQTQEFNGAARSLYDTIVPRSSFIVYRKPL"
     gene            complement(2784..3206)
                     /locus_tag="PK812_04805"
     CDS             complement(2784..3206)
                     /locus_tag="PK812_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRJ68908.1"
                     /translation="MAQTITKGFKALLNEANAQVKTLSVVEAAGMHGREDVVFVDLRD
                     PRELEREGRMPGAFHCPRGMLEFWIDPESPYAKPQFQQEKTFVFFCAGGWRSALAAKA
                     AQDMGLKPVAHISGGFGAWKQYGAPVQLPEAMPGESKA"
     gene            3400..4392
                     /gene="dctP"
                     /locus_tag="PK812_04810"
     CDS             3400..4392
                     /gene="dctP"
                     /locus_tag="PK812_04810"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HRJ68909.1"
                     /translation="MTISRRTLLGSATAAGATIALPGVLRAQEATINAVHFTPAQVGY
                     AQSFLKFAAKVNEKGKGIVQIRVRGGPEVIPFGQLGESQRTGAIDMINAPAGAYLNLV
                     PEGEAFNITTKKPAELKANGGFDLINQVYNRKGNAQLLAHVDGGRGFHVFCVDEPAQT
                     TDGSFDWKKLKVRAAPLQRDFMESLGCNVVVMGPAEVYTALERGLVNSTCYTILGYKA
                     FGWNKFTKFRIDPDFFQTDVLISMNKAKWDSLSAEAKKVLADVSAEHEASSIAENVTN
                     TENEDKQLQADGMKILKMSDAAGKAFQAKATQVSLERMTKRDPANVEALRAKFL"
     gene            4460..4969
                     /locus_tag="PK812_04815"
     CDS             4460..4969
                     /locus_tag="PK812_04815"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRJ68910.1"
                     /translation="MDRLFDWSGRLSAFLALLAKLVVGLIVVLVAADVLARNVSRSLV
                     WSVSLVEYLMIYMTFLAMPALVRAKAHVCADFIRMSLPPGLQSVVEKLVYVLCLALSA
                     YLALVATRVFIETWQNGSYDVRNFDMPRWLIFLPMVLGLWLSCIEFLRYLLGRDSIYA
                     IAAADRESL"
     gene            4966..6282
                     /locus_tag="PK812_04820"
     CDS             4966..6282
                     /locus_tag="PK812_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRJ68911.1"
                     /translation="MNLDWPLALAVMIGLVLTLMGVGVPVAFAFFATNIVCVFIFMSG
                     SASVTQMIGNASEAVSIYALTPMPLFLVMGSLFFRSGLGNDVFKAIDMCIGGLRARLS
                     YLTVISGAVFAALSGSSLANTGMMGSTMAPEMLKRGYKPKMAYGPILGAGGLAVIIPP
                     SSLAVLLGSLAQIDVGALLIAGFLPGLLLALMYVTLIRLQVALDPLGAPQYDAPDHTL
                     GEKIAALARNVFPMGVIIFAVVGTIILGIASPTESAAIGCFAVAGLLVLYRRFNWATV
                     WKSLDDAMKVSAMTFLIIAASTTFSQVFAFSGASQGLIQWLLSFGAAPMMMLLLMVLV
                     ILLLGMFMDQLSMMLITLPIFIPIAKLLGFDLVWFGLILLLVYEIGFTTPPFGLLLFV
                     MLGIAPPGTTLKTVAMAALPYILCTLLLVLLIALFPQIALFLPKLM"
     gene            6324..6599
                     /locus_tag="PK812_04825"
     CDS             6324..6599
                     /locus_tag="PK812_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3253 domain-containing protein"
                     /protein_id="HRJ68912.1"
                     /translation="MASDSDRTALLEAEVLRFAAECGPGKTFCPSDAARAVGGSHPDQ
                     WGPLMIPVRRIAVRLANEGRLVITRKGKPVDPNDFRGVYRLSQPRGD"
     gene            complement(6602..7378)
                     /gene="murI"
                     /locus_tag="PK812_04830"
     CDS             complement(6602..7378)
                     /gene="murI"
                     /locus_tag="PK812_04830"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HRJ68913.1"
                     /translation="MHILVFDSGLGGLTVYDEIRKVRPDARFTYLADDAVFPYGRLEE
                     GDLVARVLEVVGAEIRRSQPDIVVIACHTASTLVLPHLRAAYGIPFVGTVPAVKPAGL
                     ATQTKLFSVLATPGTVKRDYTRALIDEFAEGCSVTLVGCPRLASVAEAKLRGAAVDSA
                     VIAAEIAPAFVEEGGRRTDTVVLACTHYPLLLEELKALAPWPVIWIDPAPAIAKRVTQ
                     LVGKRPEGASESGPGRAIFTSRRAADRPLAAALKARDLEI"
     gene            complement(7387..8643)
                     /locus_tag="PK812_04835"
     CDS             complement(7387..8643)
                     /locus_tag="PK812_04835"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRJ68914.1"
                     /translation="MSQSSQVEVVIVGAGAAGLAAAKTLRRHNVSFVVLEARDRIGGR
                     TVTDTSLGAAFDGGATFIHFADRNPWSKFAEEYGVEARVGSWRSGGSRSFSDGVPVPA
                     EAAAARWQGRGKLWELVNEIDDSNDVSFAALVKTAPDDVAFAAKSMMRGAVGEEPERV
                     SVSDYGRLYDGSNMIVPGGYGTLVERYGADVPVSLGTPVKAMDWSGRGVRVTTASGTI
                     QAAKAIVTVPLGVLAAESIKFTPRLPDETLRAIAGMKMGALSKLALRFEGDRFGAPAD
                     MHFVETGDSAPQMSFELWPFDRDIVVCWFGADYARQINASGEASAVQHMLDRFVKIVG
                     ADARKAFRGGRHFGWSADPYALGGYSYCVPGRARARDELRRPVGERIWFAGEHVAGKA
                     SMTVGGATLSAESAAEAVYARLKAMR"
     gene            complement(8758..9567)
                     /locus_tag="PK812_04840"
     CDS             complement(8758..9567)
                     /locus_tag="PK812_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003615427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRJ68915.1"
                     /translation="MSAPVVILVRPQMAENIGMAARAMANFGLSRMRLVAPRDGWPQK
                     TRMKKGAHQAASGATHILDGAQLFSSVEEAVADLTYVLATTARERGQAKPVLTPQGAM
                     HEILEHPGAGEGVGILFGPERTGLENDEVSLADAVLTFPINPAFASLNLAQAVLLVGY
                     EWFKTAQGGQAPFAMPSQTGPARRETLFSFFAFAEKELDRVRFFWPPDRRATMVRNFR
                     NIFHRLALTEQDVRTLRGALDALVTGGKPERMAKPHPGERPAHDRESDKGT"
     gene            complement(9564..10421)
                     /locus_tag="PK812_04845"
     CDS             complement(9564..10421)
                     /locus_tag="PK812_04845"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRJ68916.1"
                     /translation="MTNDPDFRRFAFGLSTLLAEPRGFFSPYRHAAGVPGDVAVYGEI
                     EAMMREAAPAMCAVLAAIQNEAPALRALGGAAPAPRFEQDWFPRLDAAAAYALVTSGR
                     PRRIVEVGSGHSTRFMAEAARSGRIDCAITCIDPQPRAALAGLPVVWIRDVLARAHVP
                     LIDALETGDILFIDSSHILWPGTDVDTLFARLLPRLAPGVLLHVHDIFLPDGYPQAWR
                     WRGYTEQQGVAALLAGGGFEIVFASHFAVTRLAAAGLAPILNDLPLPEGALETSLWLR
                     KTANRVWRP"
     gene            10642..11853
                     /locus_tag="PK812_04850"
     CDS             10642..11853
                     /locus_tag="PK812_04850"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017964507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HRJ68917.1"
                     /translation="MAKIKVKNPVVELDGDEMTRIIWDLIKKKLIHPYLDVDLHYYDL
                     GVEYRDKTNDQVTVDSAEAIKKYGVGVKCATITPDEARVKEFNLKEMWKSPNGTIRNI
                     LGGTIFREPIICKNVPRLVPGWTQPIVVGRHAFGDQYRATDFKYPGKGTLTIKFVGED
                     GTVIEKEVFKAPGSGVAMAMYNLDDSIRDFARASFAYGLLRNYPVYLSTKNTILKAYD
                     GRFKDIFQAVFDAEFAEEFKKKGLIYEHRLIDDMVASALKWSGAYVWACKNYDGDVQS
                     DVVAQGFGSLGLMTSVLMTPDGKTVEAEAAHGTVTRHYREHQKGKETSTNSIASIFAW
                     TRGLAHRAKLDDNAELARFCTTLERVCIETVEAGFMTKDLALLVGPEQKFLSTTGFLD
                     KVDQNLQKAMI"
     gene            complement(11923..12813)
                     /locus_tag="PK812_04855"
     CDS             complement(11923..12813)
                     /locus_tag="PK812_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68918.1"
                     /translation="MSTIGIRISSPYREAVAVVISTTAILDGIKFVAGLYGSLKTDDA
                     IKKLEAHVQVLYILCRRLSLRIEEVRDELLTKIHESEYRSIYDRTVSSNSFFSQFDEK
                     NYDQIRPFLHSIIYDKYVIGDAIISQLDRDWIRLGKPEYYLAYLDLFISNELIRFSSV
                     ALVEEGKMSDEIKIGQLLQKKEKMIQKLDYAYNILAYIEQQKYEFSSSGFLECREDDD
                     GSILSCDQDYGFSLKGTDKVTIVTKVTSSPGGAAAIEAHDKALAIAKEKVHDAIVAFC
                     RPLAQEKWDSVYGEIRSSFE"
     gene            complement(12900..14123)
                     /locus_tag="PK812_04860"
     CDS             complement(12900..14123)
                     /locus_tag="PK812_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018409131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-gated ion channel"
                     /protein_id="HRJ68919.1"
                     /translation="MSRRRAHKLRVWRRRVYQILDAGFQGDHVSERLHWLLMALVTVN
                     VATSVLESVPTLAATHGRLFLAIEIASAIVFIVEYVARLWCAPEYGPISELPPWKARL
                     KYALTPGMIVDFLAIVPFFLMMFLSQDLKVLLIFRLFRFFKFARYSTGMRSLLDALYT
                     ERRALGACGVILGGLVLVSASIMHLIEHEAQPDKFGSIPDAMYWAIITLTTVGYGDVT
                     PVTPVGKIVASLTAVFGLGMVALPVGILATAFAEVIHRRDFVVTWSMVARVPLFNGLD
                     AETIAEVMRFLRSQMAQPGQVIVRRGDQAHSMYFIAGGQVEIELPGAPVVLGVGDFFG
                     EIALLSNVRRTATVRALTRSSLLVLDASDVHGLMERRPEVADRIRAIADTRLKQHQVD
                     ARGDIISDELDGQPD"
BASE COUNT         2657 a         4648 c         4213 g         2533 t
ORIGIN      
        1 gggcggcgcc tatgaggccc gcacgatggg cgcgatggcg ctgcgctgcg gcttctggat
       61 cgcggtggcc accatactgc tgagagcagg ccgggagaag gcagcgacct gaccggtcag
      121 cccgcatcct cgtcccgcat gcgcaggacg cccatctttt cagggtgata gccgcgcacg
      181 ccctcgtagc gcgcgcggat ggcgttcatg tcggccttcg catcaccggt gagggcaatt
      241 gcaggaccga tgtcgatggt gcgggtcccg aaatccagcg cggcgagcaa cagcggcaca
      301 ccggcgccaa tcgcgatgtg gtagaagccg gacttgaaat actccgtgcg cttgcgcgtt
      361 ccttcgggcg agatcgccag ccggagttcc ggcatcctgc cgaacatcgc cacggcttcg
      421 ccgacgacgc cctgcgccgc cgagcgatcg accggaatcc cgccaagcgc tttcagcacg
      481 gggcccgcgc cgaagcggaa gatctcggcc ttggcgaggt agctcaggtt catgccgtag
      541 gccatgcgcg ccagcacgaa gaacaggaaa tcccagttcg acgtgtgcga atatccgatg
      601 atcacgcact tgtccggcac gtcgcgcgcg atgaccttcc agccgaaggc atgcagcagg
      661 cccagcgcga ttttctgttt caggctcttc gcctcgacct cgcgcaccgt cagtctttca
      721 gcagcgcgcg gtacttatcg acctctttgg gcacgagcgc cgtgacatga gccggtgtca
      781 tctcctgttc gctggccggc agcgccgaca gctccttgag ccggccctgc accaacgggc
      841 tggccaccgc cttgcgcagc gcggcgttga gcctgtcgat caccggctgc ggcgttccgg
      901 caggcgcgaa gatcgcgttc cagccttcct gctggaactc gggcaggccc gcctcggcgg
      961 ccgtcggcac ctcgggatgc tttcccagcc gcaccggcga agccactgcc agcgccttca
     1021 cctgcttgcc ttccaccgca ccggaaatcg aggtgatggt gtcgcagacg ccatcgacat
     1081 ggccaccaac nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ttgaacgtca
     1201 ggttcggata gagggaatag accgcgcctg cggtaccggc attgccgacg atcagcgtat
     1261 agccatccgg cgccgccttc gccgcgcggg ccatcgcggt tgagccgccg gcgccggcaa
     1321 ggttctcgat aaggatgcgc tggcccagca cattgcccat ttcctcggcc gccagccgcc
     1381 cgatgacgtc ggacgtgccg cccggcgcga acggcacgat cagcgtgatc ggccgggacg
     1441 gatacgcgtc ctgtgcgaaa gctggcaacg ccgctgcgaa caaggcaagg gctgcgagac
     1501 gaaccatgcg gcgtttcccc ggagtgtttg tcatcttgtg acaggtggaa cggtccgggg
     1561 caagacgcgc gaaatcagag ttcgctggcg gcgatcaccc agggctcggc ttccgccgcc
     1621 ttcttccagg cctgcatggc cggcaggttg agaaccgagt ccatgtaggc gcgcgtatcg
     1681 tccgcgaccg cgatgccata gtggtggaag cggttgacga ccggcgcata catggcgtcc
     1741 gccgccgaga aggtcccgaa caggaactgg ccgctctggc cgaaacggcc ccgtgtatcc
     1801 cgccagatcg cctcgatccg gtccacatcg ctgcgcgcgg cggccggcag cgcgaagggc
     1861 ttggcgggat cgcgccggca attgtgcggg caatgggtgc gcagcgcgga aaagcccgag
     1921 tgcatctcgc aggcgatgga cctggccgtc gcacgcgccg cccggtccag cggccaaagc
     1981 cgaccacgca catgaaactc gtccaggtag tccagaatgg cgagcgattc ccagatcagg
     2041 atggcgccgt ccctcagagc cggcacgcgg cccgagggcg acgccgccag gatcttctcc
     2101 cgggtatccg gaaggtccag cggaatcacc acctcgtcga agcggatgcc catgtgcgtc
     2161 atcgccaccc agggccgcag cgaccaggac gagtaggcct tgttgccgat atagagcgtc
     2221 agggacatga aggtctcccg gtcagagcgg cttgcgatag acgatgaagg acgagcgcgg
     2281 cacgatggtg tcatagagtg agcgcgccgc gccgttgaac tcctgtgtct gccaatagac
     2341 ccggaaggcg ccgtcggcct tggccgcatc gaacacggca tccatcaatg ctcgtgcagc
     2401 accaccaccg cgcgcatgcg gatcaacata caagtcctgc aggtaacaga tgggctgcgt
     2461 gctccaggtc gaggcatgat agagatagtg cacgaagccg atcgtttcgc ccgctcgctc
     2521 ggcaacgaag gcctcgaact ttccccgttc tacgaggatt tcggcccaca gggcggcggt
     2581 ggcctccgcg ctgacgctac cccggtagaa ccggatgtag ccggcccaga gcgcgcgcca
     2641 ggccgtttcg tctccagcaa gcggtctgcg aattgttact tcggccatga cgtcccttcc
     2701 acgagcgcaa agctacgcga ccggaccggg ggcacaagca aatcttgctg attgcaccga
     2761 tcagcaaagc agatggccga acatcaggcc ttgctttcgc ccggcatggc ttcgggcaat
     2821 tgaaccggcg ctccatactg tttccaggcc ccgaaacctc ccgaaatgtg cgcaaccggc
     2881 ttcagtccca tgtcctgcgc ggccttcgcc gcaagcgccg agcgccagcc gccggcgcag
     2941 aagaagacaa aggttttctc ctgctggaac tgaggcttgg cataggggct ctccggatcg
     3001 atccagaact ccagcatgcc gcgcgggcaa tggaacgccc ctggcatccg gccctcgcgc
     3061 tcaagttcgc gcgggtcgcg caagtcgacg aacaccacat cctcgcgccc gtgcatgcca
     3121 gcggcttcga ccaccgacag ggtcttcacc tgcgcattgg cctcgttgag taacgccttg
     3181 aatcccttgg tgattgtctg tgccatagtc gcctcgtgct tgccttgacc tcgccattca
     3241 tgccgccccg ccgcgcctgt caagggccgc aacttcgcgc ttgaccctcg aagcttaaaa
     3301 cgtattctgt attcaagatg gggctcgaca agcgcttgca aggcgctcac ccgagcgtca
     3361 ccatgctgcg tcaggcagtc acaggggagg aaagcagcca tgacgatttc caggcggaca
     3421 ttgctcggca gcgcgactgc ggcaggcgcg accatcgcct tgccgggcgt tctgcgcgcg
     3481 caggaggcga ctatcaacgc cgtgcatttc acaccggctc aggtcggtta cgcgcagagt
     3541 ttcctgaagt ttgccgcgaa agtgaatgag aaaggcaagg gaatcgtcca gattcgcgtg
     3601 cgcggcggcc cggaggtgat ccccttcggc cagctcggcg agtcccagcg caccggcgcc
     3661 atcgacatga tcaatgcccc ggccggcgcg tacctgaacc tggtgccgga gggagaagcg
     3721 ttcaacatca ccacgaaaaa gcctgcggaa ctgaaggcca atggcggctt tgacctgatc
     3781 aatcaggtct acaaccgcaa gggcaatgcc cagcttctcg cccatgtcga cggcggtcgc
     3841 ggcttccacg tcttctgcgt ggatgagccg gcgcagacga cggatggcag tttcgactgg
     3901 aagaaactga aggttcgcgc cgccccgctt cagcgcgact tcatggaaag cctgggctgc
     3961 aacgtcgtgg tgatgggccc cgccgaggtc tacaccgcgc tggaacgcgg cctggtgaat
     4021 tccacttgct acacgatcct gggctacaag gcctttggct ggaacaagtt cacgaaattc
     4081 cgcatcgatc ccgatttctt ccagaccgac gtgctgatct cgatgaacaa ggccaagtgg
     4141 gattcgcttt ccgccgaggc caagaaggtg ctcgccgatg tctccgccga gcatgaagcg
     4201 tcgagcatcg ctgaaaacgt caccaacacc gagaacgagg acaaacagct tcaggccgac
     4261 ggcatgaaga tcctcaagat gtcggatgcg gccggcaagg cgttccaggc aaaggcgaca
     4321 caggtctccc tagagcgcat gaccaaacgc gatccggcca atgtcgaggc gctgagagca
     4381 aagttcctct gaggcaagat cgtcggtcct ggcgcggcca atccgcgcca ggcgcctcgg
     4441 cgcgcgaggg gaggggacgg tggatcggct gttcgactgg tcggggcgcc tcagcgcttt
     4501 cctggccttg ctggcgaagc tcgtcgtcgg gctgatcgtc gtcctggtcg ctgcggatgt
     4561 gctggcgcgc aatgtgtcgc gttcgctggt ctggagcgtc tcgctcgtcg aatacctgat
     4621 gatctacatg acgttcctcg ccatgccggc gctggtgcgg gccaaggcgc atgtctgcgc
     4681 cgacttcatc cgcatgtcgc tgccgcccgg tctccagtcc gtcgtggaga agctggtcta
     4741 cgttctgtgt ctcgccctga gcgcctatct ggcccttgtg gcgacgcgtg tcttcatcga
     4801 gacctggcag aacggctcct atgacgtgcg caacttcgac atgccgcgct ggctgatctt
     4861 cctgccgatg gtgcttggcc tctggctctc ctgcatcgaa ttcctgcgtt acctgctcgg
     4921 gcgcgattcc atctacgcca tcgctgcagc ggatcgggaa agtctatgaa cctcgattgg
     4981 ccgcttgccc ttgccgtcat gatcggactg gtgctgacgc tgatgggcgt cggcgtgccg
     5041 gtcgccttcg ccttcttcgc cacgaacatt gtctgcgttt tcatcttcat gagcggcagc
     5101 gcgtccgtga cgcagatgat cggcaacgcc agcgaagccg tgagcatcta cgcgctgact
     5161 ccgatgccgc tgttcctggt gatgggcagc ctgttctttc gctccggcct cggcaacgat
     5221 gtgttcaagg ccatcgacat gtgcatcggc ggcctgcgcg cccgcctgtc ctatctcacg
     5281 gtgatctcgg gcgctgtgtt cgcggcgctt tccggctcca gcctcgccaa caccggcatg
     5341 atgggctcga ccatggcgcc ggagatgctg aagcggggct acaagccgaa gatggcctat
     5401 ggcccgatcc tcggcgctgg cggactggct gtcatcatcc cgccgtcatc gctggcggtg
     5461 ctgctcgggt cactcgcgca aatcgacgtc ggcgcgctgc tcatcgccgg cttcctgccg
     5521 ggcctgctgc tggccctgat gtatgtcacc ctcatccgcc tgcaggtggc gctcgacccg
     5581 ctcggcgccc cccagtatga cgccccggac cacacgctgg gcgagaagat cgccgcgctg
     5641 gcgcgaaacg tcttcccgat gggcgtcatc atcttcgcgg tggtcggcac gatcatcctt
     5701 ggcattgcct cgccgacgga atcggcggcc atcggctgtt ttgccgtcgc cggactgctc
     5761 gtgctttacc ggcgcttcaa ctgggcgacg gtatggaagt cgctcgacga cgccatgaag
     5821 gtctcggcca tgaccttcct gatcatcgct gcctccacga ccttctcaca ggtcttcgcc
     5881 ttttccggcg cgtcgcaggg cttgatccag tggctcctgt ccttcggcgc cgcgccgatg
     5941 atgatgctgc tcctcatggt gctggtgatc ctgctgctcg gcatgttcat ggaccagctc
     6001 tcgatgatgc tgatcacgct gccgatcttc atccccatcg ccaagctgct gggcttcgat
     6061 ctcgtctggt tcggactgat cctgttgctt gtctatgaaa tcggcttcac gacgcccccc
     6121 ttcggcctgc tgctgtttgt catgctcggc atcgcgccgc ctggcacaac cctgaagacg
     6181 gtcgccatgg cggccctgcc ctacatcctc tgcacgttgc tgctggtgct gctcatcgcg
     6241 ctgtttccgc agattgccct gttcctgccg aaactgatgt agcggcgtct tgacccggcg
     6301 cgtcctgccg gcgacatagc cccatggcca gcgactcgga tcgaaccgcc ctccttgaag
     6361 ccgaagtgct ccgtttcgct gcggagtgcg gaccgggcaa gaccttctgc ccttccgatg
     6421 cggcgcgcgc agtcggcggc agccacccgg accaatgggg cccgctgatg attccggtcc
     6481 gccggattgc cgtgcgtctc gcgaacgaag gccgcctcgt catcacccgc aagggcaagc
     6541 cggtggaccc caacgacttc cgcggcgtgt accggctgag ccagccgcgc ggtgactgat
     6601 ctcagatttc gaggtcccgc gccttcagtg ccgcagccag gggacgatcg gctgcgcgcc
     6661 ggcttgtgaa gatcgcgcga cccggacctg actcggacgc cccctccggc cgtttgccga
     6721 cgagttgtgt cacccgcttc gcgatggcgg gcgctggatc gatccagatg accggccagg
     6781 gcgccagcgc cttcagctcc tccagcagca agggatagtg cgtgcaggcc agcaccaccg
     6841 tatcggttcg ccgcccgccc tcctccacga aggccggcgc gatctccgcc gctatgaccg
     6901 cgctgtcgac tgccgcaccc cgcagttttg cctcggcgac ggaggcaagc cgcgggcagc
     6961 cgaccagcgt caccgagcat ccctcggcaa actcgtcgat cagcgctcgg gtgtagtccc
     7021 gcttcaccgt tcctggagtc gccagcacgg agaacagctt ggtctgcgtc gccaggcccg
     7081 ccggcttcac cgccggaacg gtgccgacaa agggaatgcc ataggcggcg cgcagatgcg
     7141 gaagaaccaa cgttgacgcg gtgtggcagg cgatcaccac gatatccggc tgcgagcgcc
     7201 ggatttccgc gccgacgact tccaggacgc gcgcgaccag gtcgccctct tccagccgtc
     7261 cgtagggaaa cacggcatcg tccgcgagat aggtgaaccg cgcatccggc ctcaccttgc
     7321 ggatctcgtc gtaaacggta agtccgccca acccggaatc gaaaacgaga atgtgcattc
     7381 ccgtccctag cgcatggcct tcagtcgcgc atagaccgct tccgccgcgc tctcggcgct
     7441 gagcgtcgcg cccccgaccg tcatggacgc cttgccggcc acgtgctctc cggcgaacca
     7501 gatgcgctcg ccgaccggtc gacgcagctc gtcgcgcgcc ctggcccgcc ccggcacgca
     7561 gtaggaatag ccgcccaggg catacgggtc ggcagaccag ccgaagtgcc gccccccgcg
     7621 aaacgccttg cgcgcatcgg cgccgacgat cttcacaaaa cgatcgagca tgtgctgcac
     7681 cgcgctggcc tctccggagg cgttgatctg ccgcgcatag tcggcgccga accagcagac
     7741 cacgatgtcg cgatcgaacg gccacagttc gaaggacatc tgcggcgcgc tgtcgccggt
     7801 ctcgacgaaa tgcatgtcgg ccggcgcgcc gaagcggtcg ccttcgaacc ggagcgccag
     7861 tttggacagc gcgcccatct tcatgcccgc gatcgcgcgc agcgtctcgt cgggcagccg
     7921 gggggtgaat ttgatggatt cggcagccag tacgccgagc ggcaccgtga cgatggcctt
     7981 cgcggcctgg atcgtcccgg aggcggtcgt caccctgacc cccctgccgc tccagtccat
     8041 ggccttgacc ggcgtgccca gactgaccgg cacatcagcg ccgtaccgtt cgaccaaagt
     8101 gccgtatccg cccggcacga tcatgttgga gccatcatac agacggccat agtcggagac
     8161 cgaaacccgc tccggttcct cgcccacggc gccgcgcatc atgctcttcg cggcaaaggc
     8221 aacatcgtcg ggcgcggtct tcacgagggc cgcgaaggaa acgtcgttgc tgtcatctat
     8281 ctcgttgaca agctcccaga gcttgccgcg cccctgccag cgcgccgcgg cggcttcggc
     8341 tggaaccggc accccgtcgc tgaacgatcg cgagccgccc gagcgccagg aaccgacgcg
     8401 cgcctcgaca ccatactcct cggcgaactt ggaccagggg ttcctgtcgg cgaaatggat
     8461 gaaggtcgcg ccgccgtcga acgcagcgcc aaggctggta tcggtcaccg tccgaccgcc
     8521 gatgcggtcg cgcgcctcca gcactacgaa gctcacgttg tgccggcgca gtgtcttggc
     8581 cgcggcaaga cccgccgcac ccgcaccgac gatgacaacc tccacctgcg acgactggct
     8641 catggctctt cctgcggcaa ggacgaaggg agcggcgata gcgctgcggc gcgacaatgg
     8701 catggcgata ctctcgattc accggactct ggatagcaag cccgcgcggg cttgccatca
     8761 ggtccctttg tcgctctcgc gatcgtgagc cggccgttcg cccggatggg gcttggccat
     8821 ccgctccggc ttgcctccgg tgaccagcgc atcgagagcg cccctcaacg tgcgcacatc
     8881 ctgctccgtc aacgcgaggc gatgaaagat gttgcggaaa ttgcgcacca tcgtcgcccg
     8941 acggtccggc ggccagaaga agcggaccct gtccagttcc ttctccgcga atgcgaagaa
     9001 ggagaacagc gtttcgcgcc gcgccggtcc ggtctgtgac ggcatggcga agggcgcctg
     9061 accgccctgc gcggtcttga accactcgta gccgaccagc agcacggcct gtgcaaggtt
     9121 gagtgaggca aaggccggat tgatcgggaa ggtcagcacc gcatcggcca gcgacacctc
     9181 gtcgttctca agtcccgtgc gttccgggcc gaacaggatg cccacccctt cgcctgcccc
     9241 gggatgctcc aggatctcgt gcatcgcccc ctggggcgtg agcaccggct tggcctggcc
     9301 gcgttcgcgt gccgtggtgg ccagcacgta ggtcagatcg gcgaccgcct cctcgacgga
     9361 cgagaacaac tgcgcgccgt cgagaatatg cgttgcgccc gatgccgcct gatgcgcgcc
     9421 cttcttcatg cgcgtcttct gcggccagcc gtcgcgcggc gcgacgagac gcatccgcga
     9481 caggccgaaa ttcgccatcg cgcgcgcggc catgccgatg ttctccgcca tctgcggacg
     9541 gaccaggatg acgacaggcg cgctcatggc cgccacaccc ggttggcggt tttccggagc
     9601 cacaggctgg tttcgagcgc gccctccggc agcggcaagt cgttcaggat cggcgcgagg
     9661 cctgccgccg caagccgcgt caccgcgaaa tgcgacgcga agacgatctc gaagccgccg
     9721 ccagccagca gagcggcgac accctgctgc tcggtatagc cacgccagcg ccaggcctgc
     9781 ggatacccgt caggcaggaa aatgtcgtgg acatgcagca gaaccccggg cgccagtcgg
     9841 ggcaagaggc gcgcgaataa cgtgtccaca tccgtgcccg gccacaggat gtggctggaa
     9901 tcaatgaaca ggatgtcgcc cgtttccagc gcatcgatca gcggaacgtg cgcgcgtgcc
     9961 agcacgtccc ggatccagac caccggcagc cccgcgagcg ccgcgcgcgg ttgcgggtcg
    10021 atgcaggtga tggcgcagtc gatccggcca gagcgcgcgg cttccgccat gaaccgcgtc
    10081 gaatgcccag atccgacctc gacgatgcgc cttggccgcc cggacgtcac cagtgcatag
    10141 gctgccgccg catccagccg cgggaaccag tcctgctcga aacgcggcgc cggcgccgcg
    10201 ccgccaagcg cccgcaaggc cggcgcctcg ttttggatgg ctgccaggac ggcacacatg
    10261 gccggcgccg cctctcgcat catcgcttcg atctcgccat acacagcaac atcgcccggc
    10321 acgcccgccg catggcgata gggggaaaaa aagccccgcg gctcggcaag cagcgtcgac
    10381 agtccgaaag cgaagcgccg gaaatccgga tcgttggtca tcacgcgttc ctaatgcgga
    10441 gtgctcgacg ggtccactag ccagaacctg gccgtgcgcc tatcgcgcgg gccggagccg
    10501 ttcgcgcatt ttgccgtcac ctccgccttt cgccttgctt cccgcccgca tgtgcgggtg
    10561 ctatagcggc ccgggatgcg gctcgtcgcg gtcgggggac ggcgggagcg cgcgcatacg
    10621 gcttcttgtg acgaggaaat catggcgaag atcaaggtaa agaacccggt cgttgaactc
    10681 gacggcgacg agatgacccg gatcatctgg gacctcatca agaagaaact gattcatccc
    10741 tatcttgatg ttgatctgca ctactacgat ctgggcgtcg aataccgcga caagaccaac
    10801 gaccaggtca cagttgattc ggcggaagcc atcaagaaat acggcgtcgg cgtgaaatgc
    10861 gcgacgatca cgccggacga agcccgcgtg aaggaattca acctgaagga aatgtggaag
    10921 tcgccgaacg gcaccatccg caacatcctc ggcggcacca ttttccgcga gccgatcatc
    10981 tgcaagaacg tgccgcgtct cgtgccgggc tggacccagc ccatcgtcgt cggccgccac
    11041 gccttcggcg accagtaccg cgccaccgac ttcaagtacc ccggcaaggg cacgttgacg
    11101 atcaagttcg tcggcgagga cggcacggtg atcgagaagg aagtcttcaa ggcgccgggc
    11161 agtggcgtcg ccatggcgat gtacaacctc gacgattcga tccgcgactt cgcccgcgcc
    11221 tccttcgcct acggcctgct gcgcaactac ccggtctacc tctccaccaa gaacaccatc
    11281 ctcaaggcct acgacggccg cttcaaggac atcttccagg ccgttttcga cgccgaattc
    11341 gccgaagagt tcaagaagaa gggtctcatc tacgagcacc gcctgatcga cgacatggtc
    11401 gcctccgccc tcaagtggtc cggcgcctac gtctgggcct gcaagaacta cgacggcgac
    11461 gtgcagtcgg acgtggtggc gcagggcttc ggatcgctcg gcctgatgac ctccgtgctg
    11521 atgacgccgg acggcaagac ggtcgaggcc gaggcggcgc acggcacggt gacgcgccat
    11581 taccgcgagc accagaaggg caaggagacg tccaccaact cgatcgcctc gatcttcgcc
    11641 tggacgcgcg gtctcgccca tcgcgccaag ctggacgaca atgcggaact cgcccgcttc
    11701 tgcacgaccc ttgaacgggt ctgcatcgaa acggtcgagg ccggcttcat gaccaaggac
    11761 ctcgcgttgc tggtcggccc ggagcagaag ttcctgtcca ccaccggctt cctcgacaag
    11821 gtggaccaga acctgcagaa ggccatgatc tgagaaattg agaagggctg acgatcaaca
    11881 tcttagatcg tcagcctcaa caagagcaca aattaccgat atttactcaa acgaagatcg
    11941 aatctctcca taaacagaat cccatttttc ttgcgcaaga ggccggcaga acgccacaat
    12001 ggcgtcatga actttttctt tagcgatagc taaggcctta tcgtgggctt cgattgcggc
    12061 agcccctccg ggagagctag tgacctttgt cacgatcgtt actttatcag ttccttttaa
    12121 agagaaacca taatcttgat cgcacgacag gatcgagcca tcatcatcct ctcggcactc
    12181 taaaaatccc gatgaagaaa attcatattt ttgctgctca atgtaagcaa gtatattata
    12241 agcatagtcc aacttttgaa tcatcttttc ttttttctga agcaattgac cgatttttat
    12301 ttcatcagac atcttcccct cttcaactaa tgcaacagat gaaaatctaa ttaactcatt
    12361 cgatataaat aaatcaagat aagcaaggta atattctggt tttccaagtc ttatccaatc
    12421 tcgatctagc tgagaaatta tcgcatcacc tataacatat ttatcgtata ttattgaatg
    12481 aagaaatgga cgaatttgat cataattttt ttcgtcaaat tgactaaaga aagaatttga
    12541 actaactgtt ctatcgtaaa ttgatctata ttcagattcg tgaattttgg tcaaaagctc
    12601 gtctcgaacc tcttcaattc taagtgatag tcttctgcat aatatataca atacctgaac
    12661 gtgcgcctca agctttttaa tcgcatcatc agtttttagg ctgccataaa ggccggcgac
    12721 gaattttatg ccgtctaata tcgctgtcgt actaattacg accgctacag cttctctgta
    12781 tggagaggaa atccgaatac caattgtgct cacaggaatt cctttctgat ctagaaaata
    12841 cttaattaag gccgaattat ccaaatacaa caaagtctca gataattgtc aaggcacctt
    12901 caatccggtt ggccgtccag ttcgtcgctg atgatatcgc cacgcgcatc gacctgatgc
    12961 tgcttgaggc gcgtgtcggc aatcgcgcgg atgcggtccg ccacttcggg ccttcgctcc
    13021 atcagcccat gcacgtccga ggcatcgagg accagcaagc tggaccgcgt cagcgcccgc
    13081 acggtcgctg tgcgccggac attcgagagc agcgcgatct caccgaagaa gtcaccgacg
    13141 ccgagcacga cgggggcgcc cggcaactcg atctcgacct gtccaccggc aatgaaatac
    13201 attgaatgcg cctgatcgcc ccggcgcacg atgacctgcc caggttgcgc catctgcgag
    13261 cgcaggaacc gcatcacctc cgcgatggtt tccgcatcga gcccattgaa cagcggcacg
    13321 cgcgccacca tcgaccacgt gacgacgaag tcgcgccggt ggatcacctc ggcgaaggcc
    13381 gtcgccagaa tgccgacggg caacgccacc atgccgaggc cgaacaccgc ggtcaggctg
    13441 gccacgatct tgccgaccgg cgtcaccggc gtcacgtcgc catagccgac tgtcgtcagc
    13501 gtgatgatcg cccagtacat tgcatccgga atcgagccga acttgtccgg ctgcgcctcg
    13561 tgttcaatca ggtgcatgat cgaagccgag accagcacca gcccgccaag aatgacaccg
    13621 caggcgccaa gcgcgcgccg ctccgtgtac agcgcatcga gcagcgagcg catgccggtg
    13681 gagtagcgcg caaacttgaa gaagcgaaag aggcggaaga tcagcaggac tttcaggtcc
    13741 tgcgacagga acatcatgag gaaaaacggc acgatcgcca ggaagtcgac gatcatgccc
    13801 ggcgtcagcg catacttgag ccgcgccttc cacggcggga gttcggatat cggaccgtac
    13861 tccggcgcgc accatagccg cgccacgtac tccacgatga acacgattgc cgaagcaatc
    13921 tcgatggcga gaaacagccg gccgtgggtg gccgcaagcg ttggaaccga ttccagaacg
    13981 ctggtggcga cattcaccgt caccagcgcc atcaagagcc aatgcagccg ttcgctgacg
    14041 tgatcgccct gaaagccggc atccagaatc tggtagacgc gccggcgcca gacgcggagc
    14101 ttgtgagccc ggcgccggct caccgctcag gcccccgaca gcgcctgcgc c
//
LOCUS       DAOMXN010000036        14014 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_2387_length_14014_cov_12.544093, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000036 DAOMXN010000000
VERSION     DAOMXN010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 14014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14014
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2387_length_14014_cov_12.544093"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..949)
                     /locus_tag="PK812_04865"
     CDS             complement(<1..949)
                     /locus_tag="PK812_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRJ68920.1"
                     /translation="MTGGSIFAVALFVFVVITLMAGIRQVPQGMHYTVERFGRYNRSL
                     TPGLGLVIPYIETVGHKVVMMEQVLDVPTQEVITKDNANVQADGVVFFQILDAARASY
                     EVQSLNHALMNLTMTNIRTVMGSMDLDQLLSHRDEINSRLLRVVDAAASQWGLKVTRI
                     EIKDIIPPANLVESMGRQMKAEREKRAAVLEAEGHRQAEILRAEGHKQAEILAAEGRK
                     AAAFLDAEARERKAEAEAKATAVLSSAIASGDVAAVNYLIAEKYVGALGQFATASNQK
                     VMVVPMDTAGLAGTLAGIAEITKSATAEAAAARTRAGAVP"
     gene            complement(1041..1445)
                     /locus_tag="PK812_04870"
     CDS             complement(1041..1445)
                     /locus_tag="PK812_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68921.1"
                     /translation="MKRALIAGALYFAGAFALGFALGVLRTALVEPRVGKLLAVLAEL
                     PLMLTACWFLCRGVTEIVEVPATAGARLAMGLLAFALLLAAEAALFRLLFALRPLDDF
                     VADLVTPAGLAGLAAQGLFALFPLAQGRRAAS"
     gene            complement(1450..2487)
                     /gene="hemH"
                     /locus_tag="PK812_04875"
     CDS             complement(1450..2487)
                     /gene="hemH"
                     /locus_tag="PK812_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HRJ68922.1"
                     /translation="MTEMLPAHWPAGHPHVAHGKVGVLLLNLGTPDATDYWSMRRYLK
                     EFLSDRRVIEEPRWKWWPILNGIILTFRPRSKGRDYASIWNTERNEGPLKTITRDQAE
                     RLAERLSAQPNVIVDWAMRYANPTTESRLRALQEQGCERILLVPLYPQYAAATTATAC
                     DQAFRALMGMRWQPAIRVAPAYHDDPAYIAALAESVRAHLATLDFEPEVLLSTFHGMP
                     QKYLEQGDPYHCQCMKTGRLLREALGWDRERFVISFQSRFGNDPWLQPYTDETVAALA
                     KKGVKRLAMVAPGFSADCLETLEELDVENREIFLHNGGERFTYIPALNASKGGMDVIE
                     AVVRRELMGWA"
     gene            2606..4156
                     /locus_tag="PK812_04880"
     CDS             2606..4156
                     /locus_tag="PK812_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HRJ68923.1"
                     /translation="MTGLSRREAFKIAVAGGLAGATILATSAEEAVAQAAAQGVTPTF
                     RLLLVNDIYKMSGSAGNRGGFARLAAIARAEKAAGVPMLYAHAGDMYSPSLMSGFDQG
                     AHTVELLNVVPPDVFVPGNHEFDFGKEVYLKRRAESKSLFMAANLRNEDGSQLAGHED
                     RRIYDLGPVKVGVFGVALAATPLMSSAAGFRFSDEMTAVREQSKALRDAGADIVVAVT
                     HTDFMRDVEIQRSRLVDILLTGHDHDLRISYDGRAVMVESGEEGEYVTAIDVYCTIGQ
                     RDGKRAVTWRPAFRPIDSALVAPDPEAQAIVRKYEAELSKELDVQIGTTAVELDSRSA
                     TVRAQEAAIGNLIADAIRDSTGADVAITNGGGIRANKQYAAGATLTRRDILTELPFGN
                     STVMVEITGKDIKDALENGVSQVDARAGRFPQVSGMKVVYDAKAAAGSRIVSVEIAGK
                     PLDPAARYKVASNDFMFGGGDGYGALGRGRTLIGKTDGTLMANVVMGYVRKLGKVESK
                     VEGRIVAK"
     gene            4186..4527
                     /locus_tag="PK812_04885"
     CDS             4186..4527
                     /locus_tag="PK812_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68924.1"
                     /translation="MKMCSAGLIFAALVAAAPALAQQAKPFGPTTVPCNQADWKNCRV
                     SFDGSNVTRTYTHPRGGPTTETHAGCSASGGTISCPPGTWRTNSGSATGRAETLKVFL
                     DASGRAKGTGH"
     gene            complement(4531..5763)
                     /locus_tag="PK812_04890"
     CDS             complement(4531..5763)
                     /locus_tag="PK812_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRJ68925.1"
                     /translation="MAQFYDKLETRTPAARERALMRDLRALLAASRSKAPALKSQMKG
                     IAPETLKTRADLARIPVIRKSDLKAMQQADLPFGGLAAGRTSALMRILVSPGPIFEPE
                     GHDPDWWGAARAFFAAGFRRGDIVQNCFSYHLTPGGHIMESGARALGCPVIPGGVGNT
                     EQQLEAVALLKPAGYAGTPDFLKVLLDKGKEAGLDTSSLRKALVSGAAFPASLQKEIA
                     ERGIEAYQAYATAEFGIIAYETPARAGLVVNEGLIVEIVRPGTNEPVADGEIGEIVVT
                     RLSPEYPLLRFGTGDMSKVLPGVSPCGRTNLRLAGWLGRADQTTKVKGMFVHPGQVAD
                     VGKRHPDLGRLRLVVTRANEQDVMVLKAESETRDAGLAGAVAATLQAITKLKGAVELV
                     APGSLPADGKVIADERPV"
     gene            complement(5775..6593)
                     /locus_tag="PK812_04895"
     CDS             complement(5775..6593)
                     /locus_tag="PK812_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009756455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ68926.1"
                     /translation="MTAASDAPILSVNNIEVIYDHVILVLKGVSLTVPRGGIVAILGA
                     NGAGKTTTLKAISNLLKAERGDVTKGSIVFEGERVDKMTPNELVRRGCIQVMEGRHCF
                     GHLTIEENLLTGAFTRRDGSRAIKDDLEKVYNYFPRLRQRMKSMSGYTSGGEQQMCAI
                     GRALMSKPKMILLDEPSMGLAPQVVEEIFEIVKDLNAKEGVSFLLAEQNTNMALKYAT
                     YGYILETGRVVMDGEAKALKDNEDVKEFYLGVGEGNRRSFRDVKSYKRRKRWLA"
     gene            complement(6767..8092)
                     /locus_tag="PK812_04900"
     CDS             complement(6767..8092)
                     /locus_tag="PK812_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRJ68927.1"
                     /translation="MSLKKIALGLAAATALSNFAAAPALAQDSIYVPLLSYRTGPFGG
                     SGVPIADGMADYLRMLNERDGGIGGVKLLVEECETGYDTKKGVECYEAVKGKKPVVVN
                     PYSTGITLQLIPKAHVDKIPILAMAYGLSAAAVGETFPWVFNPPATYWDGASMFISHV
                     IATEGGADKLKGKKIGLIHLDAPYGKEPIPVLEAFAKQYGFELKLYPVPADKMQDQAS
                     QWLAIRRDRTDWIYNQGWGAMNPTAVKEAVKNGFPMDKFVAVWWASGDDDARAGEAGA
                     KGLKGLTFHGASANFPVIADIKKHVVDKGLSKSDKGKVGEHLYNRGVYNSMLIAEAIR
                     TAQQVTGKKVVSGEDVRRGLENLNITEARLKEMGMEGFAAPVKVTCADHSGHNKAYIA
                     QWDGQKWVKASDWIAPDVSKVRPLLEAAAKDYAEKNNWPKRTEACDNKS"
     gene            complement(8195..9271)
                     /locus_tag="PK812_04905"
     CDS             complement(8195..9271)
                     /locus_tag="PK812_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009467584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRJ68928.1"
                     /translation="MLYREAGLYKKTYEADMAVFPIRQDLIGVILIVAAAFVLPAFMS
                     NFMIGTVLIPFLVFSLAAIGLNLLTGYTGLLSLGTGAFMGVGAYACYKLTTAFPGVNL
                     IVWIIVSGFFSAAIGVLFGLPSLRIKGFYLAVATLAAQFFLEWCFVRIPWLYNYSVSG
                     AIDVPNRTLFGIPIAGASATPLTRYYVILVILIAMTWIASNIVHGRIGRMWMAIRDMD
                     IAAELMGIRPLQTKLLAFAVSSFYCGVAGAVMMFLWYGGGEAKDAFSINLSFNILFMV
                     IIGGLGSIVGAFFGAALLHLVPIFLKFGLPAFGFPLAGATAEHLTHLLTGLLIIFFLI
                     VEPHGLARLWATAKQKLRMWPFPY"
     gene            complement(9285..10235)
                     /locus_tag="PK812_04910"
     CDS             complement(9285..10235)
                     /locus_tag="PK812_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRJ68929.1"
                     /translation="MLYNIFIDPFVQMVQMPDLLVQTLWEGTVAGVLYALIALGFVLI
                     FKASGVFNFAQGIMVVFAGLTLVGLHEAGVPALLALILTVGVMYVLAFGIERLVLRPL
                     VNQPDIILFMATFGITFLLIGLGEFIFGGNPKKMITDELFLPKGAINIEMLGGRVTIQ
                     KLDIAAAVIAGLMVAALAVFFQSTRIGRALRAVADSHKAALSVGISLNQIWVIVWFTA
                     GLVALVTGIMWGARSDVSFGLQIIALKALPVLILGGFTSVPGAIVGGLIIGIGEKIGE
                     FYWGPLVGGGIESWLAFLIALVFLLFRPQGLFGDKIIERI"
     gene            complement(10246..11040)
                     /locus_tag="PK812_04915"
     CDS             complement(10246..11040)
                     /locus_tag="PK812_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ68930.1"
                     /translation="MTPASDIAKGAVLLKVDNVSLRFGGVKAITDVSFDIRKGEIRAI
                     IGPNGAGKTSMLNCINGFYHPQEGTITYKGVKREAMRPYVAASQGIARTFQNVALFKG
                     MSTLDNIMVGRTLKMNSPFFWQIWRHGPAMKEEVEHRKFVEEIIDFLEIEHIRKVPVG
                     KLPYGLQKRVELGRALAMQPELLLLDEPMAGMNLEEKEDMCRFILDVSSQYGTTIALI
                     EHDMGVVMDLSDRVVVLEYGRKIADGTPDQVKADQTVIDAYLGVGH"
     gene            complement(11037..12926)
                     /locus_tag="PK812_04920"
     CDS             complement(11037..12926)
                     /locus_tag="PK812_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRJ68931.1"
                     /translation="MRFKDMGIWREWTWSQIAKLVRAYAIGLQDLGLKRGDKVAIVGS
                     NRPKLYWSIMAAQSLGAIPVPVYADAVADEMAYVLDHAEVSIAAVQDQEQVDKLISVK
                     DRVPSLGHILYDEPRGLRDYDHAKLHPIDAIIERGTAILHEGPDIAAKFERTVAEGKG
                     EDVSIILYTSGTTGRSKGVMLTASGCINAARDSARFDGLHERDEVIAYLPLAWVGDHY
                     LNYAQQMVVGFCVNCPESPETAVSDRREIGTTYAFAPPRVFEGMLTSIMIRMEDAGWL
                     KKKMFHHFLGVAKKWGEKILNGEAVPLTARLHYGLGELMVYGPLKNVLGLSTVRIGYT
                     AGEAIGPELFAFFRSIGLNLKQLYGQTEAFLLVTAQPNGQIRPDTVGPVMPNVDLKVS
                     PDGEVLYKSPGMFKGYYKEPEKTAETLTPDGYVRTGDAGFLDATGHLKIIDRIKDVGK
                     LKSGAMFAPKYIENKLKFFPNIKEAVAFGQDHEFVTCFINIDMTAMGNWAERNNVAYA
                     SYQELAGHPQVYEIIKGHVDEVNRSLATEPMMAAAQIHRFLILHKELDADDGEVTRTQ
                     KVRRGFVAERYAPLINGFYSGAKEVDISTQVTFEDGRKGAISARVKIADLTPAPIAVA
                     QGKAA"
     gene            complement(13181..13855)
                     /locus_tag="PK812_04925"
     CDS             complement(13181..13855)
                     /locus_tag="PK812_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRJ68932.1"
                     /translation="MISLETLDRIAFWARELAPDERVRSAKGIVERTVVSGAYLIHKG
                     DRYDPWVGVIDGLLKLSTTSASGKTVTLAGISSGGWFGEGSMLKNEARRYDVTALRGS
                     KIALMDRATFLWLFEHSVGFNRFLVRQFNERLGQFMAMIEQERMLDSTGRVARAIAAL
                     LNPTFYPDAGLHVSISQEEVGALAGLSRQSANSALRLLEEAGLIRSEKGGYAVSNLEK
                     LARFGE"
BASE COUNT         2402 a         4645 c         4534 g         2433 t
ORIGIN      
        1 caggcacggc gccggcgcgc gtccgggccg ccgcggcctc cgccgtggct gatttcgtga
       61 tctctgcgat gcccgcgagc gtgccggcga ggccggccgt gtccatcggc accaccatca
      121 ccttctgatt gctggcggtc gcgaactggc cgagcgctcc gacatacttc tcggcgatga
      181 ggtaatttac cgccgcgacg tcgcccgagg cgatcgccga tgacagcacg gccgtggcct
      241 tggcctcggc ctcggccttg cgctcgcgtg cttcggcatc gaggaaggca gccgccttgc
      301 ggccctcagc cgccaggatt tccgcctgct tgtggccctc ggccctgagg atttcggcct
      361 gccggtggcc ctcggcctcg agaacggcgg cgcgcttctc gcgctcggcc ttcatctgcc
      421 ggcccatgct ctcgaccagg ttcgccggcg ggatgatgtc cttgatctcg atgcgcgtca
      481 ccttcaggcc ccactggctc gccgccgcgt cgaccacgcg caacaggcgc gagttgatct
      541 cgtcgcggtg cgacagcagc tgatccaggt ccatcgagcc catgaccgtg cggatgttgg
      601 tcatcgtcag gttcatcagc gcgtggttga ggctctgcac ctcgtaggag gcccgcgccg
      661 catcgaggat ctggaagaac accacgccat ccgcctggac attggcgttg tccttggtga
      721 tcacctcctg ggtcggaacg tcgagcacct gctccatcat caccactttg tggccgacgg
      781 tctcgatgta gggaatgaca aggccaaggc ccggggtcag cgagcgattg tagcggccga
      841 agcgctctac cgtgtagtgc atcccctgcg gcacctgccg gatgccggcc atcagggtga
      901 tcacgacaaa cacgaagagc gcgaccgcaa agatggagcc gcccgtcagg tcaatcatct
      961 tcattctccc gtgaatcgct cttctccgcc ccgccacaag gtgggcgctc gttcgccaat
     1021 tcacaagggg caagtgcggc tcaggacgcg gcccgcctgc cctgcgccag cggaaacagg
     1081 gcgaacagcc cctgggcggc gagtccggcc aatccggccg gcgtgaccag atcggcgacg
     1141 aagtcgtcga gggggcgcaa ggcaaacagc aggcggaaca gcgcggcttc cgccgcgagc
     1201 agcagggcga aggccagcag ccccatcgca aggcgcgcgc cggcggttgc cggcacctcg
     1261 acgatctcgg tcacgccccg gcacaggaac caacacgccg tcagcatgag gggaagctcg
     1321 gcgaggacgg cgaggagctt gccaacccgc ggctcgacca gcgccgtgcg caacaccccc
     1381 agggcaaagc cgagcgcgaa ggcaccggcg aaatagagcg caccggcgat cagcgcgcgc
     1441 ttcatgggct cacgcccagc ccatcaactc gcgtcgcacc acggcctcga tcacatccat
     1501 gccgcccttc gaggcgttga gcgcgggaat gtaggtgaag cgctcgccac cgttgtgcag
     1561 gaagatctcg cggttctcga catcgagttc ctccagcgtt tcgagacaat ccgccgagaa
     1621 gccgggagca accatggcca gccgcttcac gccctttttc gccagcgccg ccaccgtctc
     1681 gtccgtgtag ggctgcaacc acggatcgtt gccgaagcgc gactggaacg agatgacgaa
     1741 gcgctccctg tcccagccca gcgcctcgcg cagcaggcgt ccggtcttca tgcactggca
     1801 gtgataggga tcgccctgtt cgagatactt ctgcggcatg ccatggaagg tcgagagcag
     1861 tacctccggc tcgaagtcca gggtcgcgag atgggcgcgc acgctctcgg ccagcgccgc
     1921 gatgtaagcg ggatcgtcgt gataggctgg cgcaacgcgg atcgccggct gccagcgcat
     1981 gcccatcagc gcgcggaaag cctggtcgca agccgtcgcc gtggtcgcgg ccgcgtattg
     2041 cggatagagc ggcaccaaga ggatgcgctc gcagccctgt tcctgcaggg cgcgcaggcg
     2101 gctttccgtg gtcggattgg cgtagcgcat ggcccagtcg acgatcacgt tgggctgagc
     2161 agaaagccgt tcggccagcc tttcagcctg atcgcgcgtg atggtcttca gcggcccctc
     2221 gttgcgctcg gtgttccaga tcgaggcgta gtcacgtccc ttcgagcgcg gccggaaggt
     2281 caggatgatc ccgttgagga tcggccacca tttccagcgc ggctcctcga tcacgcggcg
     2341 gtccgagagg aactccttca gatagcggcg catggaccag tagtcggtcg catccggcgt
     2401 gccgaggttg agcagcagga cgccgacctt gccgtgggcg acgtgggggt ggccggccgg
     2461 ccagtgggcg ggaagcattt cggtcatgcc cgctatctag aacaattctt ggatatcgcc
     2521 agtgcacgaa ggcgtcacag ccttgtcatg ggcgcttgcc agacgcatga ttgcatacag
     2581 aacctctatc cgtagggagt accccatgac cggcctttcc cggcgcgaag ccttcaagat
     2641 tgcggtagcc ggcggcctgg ccggcgcgac catcctggcg accagcgccg aggaggcggt
     2701 ggcgcaggcc gcggcgcagg gcgtgacgcc gaccttcagg cttctgctcg tcaatgacat
     2761 ctacaagatg agcggatcgg cgggaaatcg tggcggtttt gcccgcctcg cggccatcgc
     2821 ccgggccgag aaggctgcgg gcgtgccgat gctctacgcc catgccggag acatgtactc
     2881 gccctcgctg atgtccggct tcgatcaggg cgcgcatacg gtggaactgc tcaacgtggt
     2941 gccgccggac gtgttcgttc cgggcaacca cgagttcgac ttcggcaagg aggtctatct
     3001 caagcgccgc gccgaatcga agtcgctgtt catggccgcc aatctgcgca acgaggacgg
     3061 atcgcagctt gccggccacg aggaccgccg gatctatgac ctcggaccgg tcaaggtcgg
     3121 tgtcttcggt gtggccctgg ccgcgacgcc gctgatgtcc tccgccgccg gcttcaggtt
     3181 ctccgacgag atgacggcgg tgcgcgaaca gtcgaaggcc ctgcgcgatg ccggcgccga
     3241 catcgtcgtc gcggtgacgc acaccgactt catgcgcgac gtcgagatcc agcgctcgcg
     3301 cctggtcgac atcctgctca ccggtcacga ccacgacctg cgcatctcct atgacggccg
     3361 tgcggtgatg gttgagagcg gcgaggaggg cgagtacgtc accgccatcg acgtttactg
     3421 caccatcggc cagcgcgacg gcaaacgcgc cgtcacctgg cgcccggcct tccgcccgat
     3481 cgattccgcc ctcgtcgctc cggacccgga agcgcaggcc atcgtcagga aatacgaggc
     3541 cgagctgtcg aaggagctgg atgtccagat cggcacgacc gccgtcgagt tggacagtcg
     3601 ctccgccacg gtgcgcgcgc aggaggccgc catcggcaac ctcatcgccg acgccatacg
     3661 cgactcgacc ggcgccgatg tcgccatcac caacggcggc ggcatccggg ccaacaagca
     3721 atatgccgcc ggcgcgacgc tcacccgccg cgacatcctc accgaactgc ccttcggcaa
     3781 ttccaccgtc atggtggaga ttaccggcaa ggacatcaag gatgcgctgg agaacggcgt
     3841 cagccaggtc gatgcgcgtg ccggccgatt cccgcaggtc tccggcatga aggtggtcta
     3901 cgacgccaag gctgccgccg gcagccgcat cgtctcggtc gagatcgccg gcaagccgct
     3961 cgacccggcc gcgcgctaca aggttgcgtc caatgacttc atgttcggcg gcggcgacgg
     4021 ctacggcgcg cttgggcgcg ggcgcacgct gatcggcaag accgacggca cgctcatggc
     4081 caatgtggtg atgggctatg tgcgcaagct cggcaaggtc gagagcaagg tcgagggccg
     4141 catcgtcgcg aagtgatccg ctgggaagga aacgggggaa ctcccatgaa gatgtgcagt
     4201 gcaggcttga tcttcgcggc gctggtcgcc gccgccccgg ctctggcgca acaggcaaag
     4261 ccctttggcc ccacgacggt gccctgcaac caggcggact ggaagaactg ccgcgtcagt
     4321 ttcgacggct cgaacgtcac gcgcacctac acgcatccgc gcggcggccc gaccaccgag
     4381 acccacgccg gctgctcggc ttcgggagga accatttcct gcccgcccgg aacctggcgc
     4441 acgaacagcg gcagcgcgac gggacgcgcc gagacgctca aggtctttct ggatgcctcc
     4501 ggccgggcca agggcaccgg ccactgagtt ttacaccggc cgctcgtcgg cgatcacctt
     4561 gccgtccgcc ggcagcgaac cgggcgcgac cagttccacc gcgcccttga gcttggtgat
     4621 ggcctgaagc gtcgccgcca ccgcaccggc aaggccggcg tcgcgggttt cgctttccgc
     4681 cttcagcacc atcacatcct gctcgttggc acgcgtgacg acgagacgca ggcggccaag
     4741 gtccgggtgg cgtttgccca cgtctgccac ctgtccggga tgcacgaaca tgcccttgac
     4801 cttggtggtc tggtcggcgc gtccgagcca gcccgccagg cgcaggttgg tgcgcccgca
     4861 ggggctgacg cccggcagga cctttgacat gtcgcccgtg ccgaagcgca gaagcgggta
     4921 ttccggcgaa agccgcgtca ccacgatctc gccgatctcg ccgtccgcca ccggttcgtt
     4981 ggttccgggc ctgacgatct cgacgatcag cccctcgttg acgacaagcc cggcccgggc
     5041 cggcgtctca taggcgatga tgccgaattc cgccgtggca taggcctgat aggcctcgat
     5101 gccgcgctcg gcgatctcct tctggagcga agccggaaag gccgcgccgg aaaccagcgc
     5161 cttgcgcagg ctggatgtgt cgagacccgc ttccttgccc ttgtcgagca gcactttcag
     5221 gaaatccggc gtgccggcgt agcctgcggg cttcagcagg gcgacggcct caagctgttg
     5281 ttcggtattg ccgacgccgc ccggaatgac cggacagccg agcgcccttg cgcccgattc
     5341 catgatgtgg ccgcccgggg tcaggtgata ggagaagcag ttctggacga tgtcgccgcg
     5401 ccgaaaccct gccgcgaaga aggcgcgcgc cgcgccccac cagtccggat cgtgaccttc
     5461 cggctcgaag atcggccccg gcgagaccag gatgcgcatg agagcggagg tccggcccgc
     5521 cgccagcccg ccgaacggca ggtcagcctg ctgcatcgcc ttgaggtccg acttgcggat
     5581 caccggaatg cgcgcaaggt ccgcgcgggt cttcagcgtc tccggggcga tgcctttcat
     5641 ctgggatttc agcgcaggcg ccttgctccg gctcgcggcc agaagggcgc gcaggtcgcg
     5701 catcagcgcg cgttcgcgcg cggcgggggt gcgcgtttcg agtttgtcgt agaactgcgc
     5761 catgatctgc cgcctcaggc cagccagcgc ttgcggcgct tgtagctctt cacgtcgcgg
     5821 aaggaccggc ggttgccctc gccgacgccg agatagaatt ccttcacgtc ctcgttgtcc
     5881 ttgagcgcct tggcctcgcc gtccatgaca acccggccgg tctcgaggat gtagccgtag
     5941 gtcgcgtatt tcagcgccat gttggtgttc tgctcggcca ggaggaagga gacgccctct
     6001 ttggcgttca gatccttcac gatctcgaag atttcctcca ccacctgcgg cgcaagcccc
     6061 atggacggtt cgtccagcag gatcatcttc ggcttcgaca tcagcgcccg gccgatggcg
     6121 cacatctgct gttcgccgcc ggaggtgtag ccggacatcg acttcatgcg ctggcgcagc
     6181 cgcgggaagt aattgtagac cttctcgagg tcgtccttga tcgcccgcga cccgtcgcgc
     6241 cgcgtgaagg cgccggtcag caggttttcc tcgatggtca ggtggccgaa gcagtgccgg
     6301 ccctccatca cctggatgca cccgcgccgc accagttcat tcggcgtcat cttgtcgacg
     6361 cgctcgccct cgaacacgat ggagcccttg gtcacgtcgc cgcgctccgc cttcagcagg
     6421 ttggagatcg ccttgagcgt ggtggtcttg ccggcgccgt tcgcgccgag gatcgcgacg
     6481 atgccgccgc gcggaaccgt gagcgacacg cccttcagca cgaggatcac gtgatcgtag
     6541 atcacctcga tgttgttcac cgagaggatg ggagcgtcgg aagcggcggt catggctttg
     6601 ccctgggagt gtgacgcaac gtgcgaaggg tccatcgccg gtactggcat gccccctcgt
     6661 tgaggcccgc ccgggcccac aaagccccgg gcggacggtc ttggtgtgcg gtcgaagtca
     6721 tcggaccggc gatcggcctt agccgaccgc cggtcgccat cagcccttac gacttgttgt
     6781 cgcaggcttc ggtgcgcttc ggccagttgt tcttctcggc atagtccttg gccgccgcct
     6841 cgagcagcgg acgcaccttg gagacgtccg gcgcgatcca gtccgaggcc ttcacccact
     6901 tctggccgtc ccactgcgcg atataggcct tgttgtggcc ggagtgatcg gcgcaggtca
     6961 ccttcaccgg cgcggcgaag ccttccatgc ccatctcctt gaggcgcgcc tcggtgatgt
     7021 tgaggttctc aaggccgcgg cgcacgtcct cgccggagac caccttcttg ccggtcacct
     7081 gctgcgcggt gcggatcgcc tcggcgatca gcatcgagtt gtagacgccg cggttgtaga
     7141 ggtgctcgcc gaccttgccc ttgtcggact tggacaggcc cttgtcgaca acgtgcttct
     7201 tgatgtcggc gatcaccggg aagttggccg aggcgccgtg gaaggtcagg cccttcaggc
     7261 ccttggcgcc ggcttcgccc gcgcgggcat cgtcatcgcc cgaggcccac cacaccgcga
     7321 cgaacttgtc catcgggaag ccgttcttca ccgcttcctt cacggccgtc gggttcatcg
     7381 cgccccagcc ctggttgtag atccagtcgg tgcggtcgcg gcggatcgcc agccactgcg
     7441 aggcctggtc ctgcatcttg tcggccggga ccggatagag cttcagctcg aagccgtact
     7501 gcttggcgaa ggcttcgaga accgggatcg gctccttgcc gtagggcgcg tcgagatgga
     7561 tcaggccgat cttcttgccc ttcagcttgt cggcgccgcc ctcggtggcg atgacatgcg
     7621 agatgaacat cgaggcgccg tcccagtagg tcgccggcgg gttgaacacc cacgggaagg
     7681 tctcgccgac ggcggccgcc gagaggccgt aggccatggc gaggatcggg atcttgtcga
     7741 catgcgcctt cgggatcaac tgcagcgtga tgccggtgga ataggggttc accaccaccg
     7801 gcttcttgcc cttgaccgcc tcgtagcact cgacgccctt cttggtgtcg tagccggtct
     7861 cgcactcctc gacgaggagc ttcacgccgc cgatgccgcc gtcgcgctcg ttgagcatgc
     7921 gcaggtagtc agccatgcca tcggcgatcg gcacacccga gccgccaaac ggaccggtgc
     7981 ggtaggacag cagcggcaca tagatggaat cctgcgccag cgcgggggcg gcggcgaagt
     8041 tcgacagcgc cgtcgcagcc gcaaggccca gagcgatttt cttgagtgac atcgggtttt
     8101 tcctccggtt tttcggaacg ccgccccggt ctcgggaacg gttggctcca atgatggtat
     8161 tcgaacgcac gtcttcaagc cccgccaagt cgcctcagta ggggaaaggc cacatcctca
     8221 gcttctgctt tgcggtcgcc cacagacggg caagaccatg cggctcgacg atgaggaaga
     8281 agatgataag caggccggtg agcaggtggg tgagatgttc ggcggtcgcg ccggcgagcg
     8341 ggaagccgaa ggccggcagg ccgaacttca ggaagatcgg caccaggtgc agcagcgcgg
     8401 cgccgaagaa cgcacccacg atgctgccga gcccgccgat gatcaccatg aacaggatgt
     8461 tgaacgaaag gttgatcgag aacgcgtcct tcgcctcgcc gccaccgtac cacaggaaca
     8521 tcatcacagc gccggcgacg ccgcagtaga aggacgagac ggcgaaggcc agaagctttg
     8581 tctgcagcgg gcggatgccc atcagctcgg cggcgatgtc catgtcgcgg atcgccatcc
     8641 acatgcggcc gatgcggccg tgcacgatgt tggaggcgat ccaggtcatg gcgatcagga
     8701 tgacgaggat cacgtagtag cgcgtcagcg gcgtcgcgct cgcgccggcg atcggaatgc
     8761 cgaacagcgt ccggttgggc acgtcgatcg cgcccgagac ggagtagttg tagagccagg
     8821 gaatgcgcac gaagcaccat tccaggaaga actgcgccgc cagagtcgcc accgccaggt
     8881 agaatccttt gatgcgcagg ctcggcagtc cgaaaagcac gccgatcgcc gccgagaaga
     8941 agccggagac gatgatccag acgatcaggt tcacgcccgg gaaggcggtg gtcagcttgt
     9001 agcaggcata ggccccgacc cccatgaagg caccggtgcc cagggacaaa agaccggtgt
     9061 agcccgtcag cagattgagc ccgatcgccg ccagcgagaa caccaggaac gggatcagca
     9121 ccgtgccgat catgaagttg ctcatgaacg ccggcagcac gaaagcggcc gcgacgatga
     9181 ggatgacccc gatcagatcc tgccggatcg ggaacaccgc catgtcggct tcgtaggtct
     9241 tcttgtatag gccggcctcg cggtacagca tgaggtcagc tccttcaaat ccgttcgatg
     9301 atcttgtcgc cgaacaggcc ctgcggcctg aacagcagga aaacgagagc gatcaggaag
     9361 gcgagccagc tttcgatgcc gccgcccacc agcgggcccc agtagaattc gccgatcttc
     9421 tcgccgatgc cgatgatcag cccgccgacg atggcgccgg gcaccgaggt gaagccgccg
     9481 aggatcagca ccgggagcgc cttgagggcg atgatctgca agccgaagga cacgtcggaa
     9541 cgggcgcccc acatgatgcc ggtcaccagc gccacgaggc cggcggtgaa ccacacgatc
     9601 acccagatct ggttgagcga gatgccgacc gagagcgccg ccttgtgact gtcggcaacg
     9661 gcgcgcagcg cgcggccgat gcgggtcgac tggaagaaca ccgccagcgc cgccaccatc
     9721 agcccggcaa tcaccgccgc ggcgatatcg agcttctgga tcgtcacgcg cccgcccagc
     9781 atctcgatat tgatcgcgcc cttcggcagg aacaactcat ccgtgatcat cttcttcgga
     9841 ttgccgccga agatgaactc cccgaggccg atcagcagga aggtgatgcc gaacgtcgcc
     9901 atgaacagga tgatgtcggg ctggttcacc agcggcctca gcaccagccg ttcgatgccg
     9961 aaggccagca cgtacatcac gccgacggtg aggatcagcg ccagcagcgc cgggacgccg
    10021 gcctcgtgca ggcccaccaa cgtcaggccg gcgaacacga ccatgatgcc ctgggcgaag
    10081 ttgaacacgc cggacgcctt gaagatcagc acgaagccga gcgcgatcag cgcgtagagc
    10141 acgccggcca ccgtgccttc ccacagcgtc tgcaccagaa gatccggcat ctggaccatc
    10201 tgcacgaagg gatcgatgaa aatgttgtaa agcatcgtct gtccctcagt gcccgacgcc
    10261 gagataggcg tcgatgaccg tctggtccgc cttcacctga tccggcgttc catcggcgat
    10321 cttgcggccg tattccagca cgacgacgcg gtcggacagg tccatcacca cccccatgtc
    10381 atgctcgatc agcgcgatcg tcgtgccgta ctggctcgaa acgtcgagga tgaagcggca
    10441 catgtcctcc ttctcctcta ggttcatgcc cgccatcggc tcgtccagca gcagcagctc
    10501 gggctgcatc gccagcgccc ggcccagttc cacgcgcttc tgcaggccgt aaggcagctt
    10561 gccgaccgga accttgcgga tgtgctcgat ctcgaggaag tcgatgatct cctcgacgaa
    10621 cttgcgatgc tccacttctt ccttcatcgc cgggccatgc cgccagatct gccagaagaa
    10681 gggggagttc atcttcagcg tgcgcccgac catgatgttg tcgagcgtgc tcatgccctt
    10741 gaacagcgcc acgttctgga aggtgcgggc gatgccctgg cttgccgcca cgtagggtcg
    10801 catcgcctcg cgcttcacgc ccttgtaggt gatggtgcct tcctgcggat gatagaaccc
    10861 gttgatgcag ttcagcatcg aggtcttgcc cgccccgttc ggtccgatga tggcgcggat
    10921 ctcgcccttg cggatgtcga acgagacgtc ggtgatcgcc ttcacgccgc cgaagcgcag
    10981 cgagacgttg tcgaccttga ggagtaccgc gcccttggcg atgtcgctgg cgggagtcat
    11041 gctgcttttc cttgggcgac ggcgatcggc gccggtgtca ggtcggcgat cttgacgcgg
    11101 gcggaaatgg cgcccttgcg accgtcctcg aaggtgacct gcgtcgagat gtccacttcc
    11161 ttggcgccgc tgtagaaccc gttgatcagc ggggcatagc gttcggcgac gaagccgcgc
    11221 cgcaccttct gcgtgcgcgt cacctcgccg tcgtcggcgt cgagttcctt gtgcaggatc
    11281 aggaaccggt ggatctgcgc ggcggccatc atcggctcgg tggcgagcga gcggttcacc
    11341 tcatccacat ggcccttgat gatctcgtag acctgcgggt ggccggcgag ttcctgatag
    11401 gacgcatagg cgacgttgtt gcgctccgcc cagttgccca ttgccgtcat gtcgatgttg
    11461 atgaagcagg tcacgaactc atggtcctgg ccgaaggcca ccgcttcctt gatgttgggg
    11521 aagaacttca gcttgttctc gatgtacttc ggcgcgaaca tggcgccgga cttcagcttg
    11581 cccacgtcct tgatgcggtc gatgatcttg agatggccgg tggcgtccag gaagccggcg
    11641 tctccggtcc gcacatagcc gtccggcgtc agggtttcgg ccgtcttttc cggttccttg
    11701 tagtagccct tgaacatgcc gggcgacttg tagagcacct cgccgtccgg cgagactttc
    11761 aggtcgacgt tcggcatgac gggcccgacc gtgtcggggc ggatctggcc gttgggctgc
    11821 gccgtcacca gcaggaaggc ctcggtctgg ccgtaaagct gcttgaggtt gaggccgatc
    11881 gagcggaaga aggcgaagag ctccggcccg atcgcctcgc cggcggtata accgatgcgc
    11941 acggtcgaca gcccgagcac gttcttcagc ggcccgtaga ccatcaattc gccgagcccg
    12001 tagtgcagcc gcgcggtcag cggcaccgct tcgccattga ggatcttctc gccccatttc
    12061 ttggcaacgc cgaggaaatg gtggaacatc ttcttcttca gccagcccgc gtcctccatc
    12121 cggatcatga tggaggtgag catgccctcg aaaacgcgcg gcggcgcgaa ggcataggtc
    12181 gtgccgatct cgcggcgatc ggagacggcg gtctccgggc tttccgggca gttcacgcag
    12241 aaaccgacca ccatctgctg ggcatagttg aggtagtggt cgccgaccca ggcgagcggc
    12301 agataggcga tcacctcgtc gcgttcatgc agcccgtcga acctggcgct gtcgcgcgcc
    12361 gcattgatgc agccgctggc cgtcagcatc acgcccttcg aacggccggt cgtgcccgag
    12421 gtataaagga tgatcgagac gtcctcgccc ttgccctcgg ccaccgtccg ctcgaatttc
    12481 gccgcgatgt ccggcccctc gtgcaggatg gccgtgccgc gctcgatgat ggcgtcgatc
    12541 ggatgcagct tcgcatggtc gtagtcgcgc aggcccctgg gctcgtcgta gaggatatgc
    12601 ccgaggctcg gcacccggtc cttcaccgag atgagcttgt caacctgctc ctgatcctgc
    12661 accgccgcga tcgaaacctc ggcgtggtcc agcacatagg ccatctcgtc ggccacggcg
    12721 tcggcgtaga ccggcacggg gatggcgccc agagactgcg ccgccatgat cgaccagtag
    12781 agcttggggc gattcgaccc gacgatggcc accttgtcgc cgcgcttcag gccaaggtcc
    12841 tgcaggccga tggcgtaggc gcgcaccagc ttggcaatct ggctccaggt ccattcgcgc
    12901 cagatgccca tgtccttgaa ccgcatggcc gggcgctcgg ggcgcacctt ggcatggtgg
    12961 gcgatccatt tgggaaaggt gtcgagtccc tgctcgggcg tgctcgtcaa cgcgctgcct
    13021 ccctggcgaa atattcccgg accgcgttcc ggtccttcta tctggttgtg caatgtgcag
    13081 tctggagccg gaaattcaag cggccccttg acagacaaag ggatgatctg gtgttaggct
    13141 acccgattat gttgcagcgc atggcgtgca aattctggcc ctattcgccg aagcgggcca
    13201 gtttttcgag attgctcacc gcgtagccgc ccttctcgct gcggattaga ccggcttcct
    13261 ccaaaagtct aagcgcggaa ttggcgctct ggcgcgacag gccggccagc gcgccgacct
    13321 cctcctggct gatcgacaca tgcaggccgg catccggata gaacgtcggg ttgagcagcg
    13381 ccgcgatggc gcgcgccacc ctgcccgttg aatccagcat gcgctcctgc tcgatcatcg
    13441 ccatgaactg cccgagccgc tcgttgaact ggcgcaccag gaagcggttg aagccgaccg
    13501 agtgctcgaa cagccagagg aacgtcgccc ggtccatcag cgcgatcttc gagccacgca
    13561 atgcggtcac gtcgtagcgg cgcgcctcgt tcttcagcat cgagccttca ccgaaccagc
    13621 cgccggagga gattccggcg agtgtcaccg tcttgcccga cgcggaggtg gtcgacagct
    13681 tgagcagtcc gtcgatgacg ccgacccagg ggtcgtagcg gtcgcccttg tggatcaggt
    13741 aggcgccgga gacaaccgtc cgctcgacga tacccttcgc ggagcgcacg cgctcgtccg
    13801 gcgccagctc gcgcgcccag aaggcgatcc ggtcaagggt ttccagcgaa atcacgccgc
    13861 atcatgcgat gcgccggacc gcgaaacaag tcgccgttca gcgcacgcgg aacgctccat
    13921 ggcagccggc gcagcgcgac gtcaggttgg acagtgcccc gaccactgcc ttgtagtcgg
    13981 ccgcggtcgg cggcgtggac gcggctgcgg ccac
//
LOCUS       DAOMXN010000037        13954 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_2401_length_13954_cov_11.761062, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000037 DAOMXN010000000
VERSION     DAOMXN010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 13954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13954
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2401_length_13954_cov_11.761062"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /gene="odhB"
                     /locus_tag="PK812_04930"
     CDS             <1..868
                     /gene="odhB"
                     /locus_tag="PK812_04930"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HRJ68933.1"
                     /translation="NNMSVDQVSGSGKRGQVLKGDVLEAIAKGAASQPAETPKVAEAP
                     KSPRAPSAGDDAAREERVRMTKLRQTIARRLKDAQNTAAMLTTFNEVDMTNVMALRGQ
                     YKDLFEKKHGVKLGFMGFFTKAVVQALKELPGVNAEIDGTDVVYKNYYHVGIAVGTDK
                     GLVVPVVRDADRLGIAGIEKTIADFGKRARDGQLKMEEMTGGTFTITNGGIYGSLMST
                     PILNAPQSGILGMHKIQDRPMVVGGKIEIRPMMYLALSYDHRIVDGKEAVTFLVRVKE
                     SLEDPARLVMDL"
     gene            924..1667
                     /locus_tag="PK812_04935"
     CDS             924..1667
                     /locus_tag="PK812_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRJ68934.1"
                     /translation="MTKVAIVTGGSRGIGRACCLRLADAGYAVVVNYTSAKAAADEVV
                     AEIAAKGGKAVAVKGDVASEADVLAIFAAADRLGPLAALVNNAGVVDVMTRVDQVSVT
                     RLERMFSINVIGSFLCAREAVKRMSTKHGGNGGGIVNISSVAAELGSPGWYVDYAASK
                     GAIDTMTKGLGLEVAAEGIRVNGVRPGIIATDIHASGGMPDRVAQMRPLLPMKREGSA
                     DEVAQVVLWLLSDAASYITATTINVSGGR"
     gene            1691..3091
                     /gene="lpdA"
                     /locus_tag="PK812_04940"
     CDS             1691..3091
                     /gene="lpdA"
                     /locus_tag="PK812_04940"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012453494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRJ68935.1"
                     /translation="MSYDLIVIGTGPGGYVCAIRAAQLGLKTAVIEKRKTHGGTCLNV
                     GCIPSKALLHASELFDEAGHGFAAFGIDVPKPKLNLAQMMKHKQETIDANVTGVGFLL
                     KKNKVTAYHGLGTILAPGKVQVTADNGASEILETKAIVIATGSEVMPLPGVEIDEKQI
                     VSSTGALDLPKVPGKLVVIGAGVIGLELGSVWRRLGADVEVIEFLDRILPGMDGEASK
                     QFQRMLEKQGIAFRLGAKVTGVDKIKGGLKVKVEPAKGGEASTIGADVVLVAIGRRPF
                     TQGLGLENAGVALDARGRIVTDSHYATNVPGIYAIGDVIAGPMLAHKAEDEGVALAEI
                     LAGKAGHVNYGVIPGVVYTFPEVASVGKTEEELKAEGVAYAVGKFPFTANGRARANRT
                     TDGFVKVLADKASDRVLGVHIVGAGAGEMIHEAAVLMEFGGSSEDLARTCHAHPTMSE
                     AVKEAALAVEKRAIHM"
     gene            3192..3530
                     /locus_tag="PK812_04945"
     CDS             3192..3530
                     /locus_tag="PK812_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68936.1"
                     /translation="MRRFVVSCLKIILDIGFWGVVLGSMAAGYMLGVSNSAAAIMLGD
                     GLWAGLLGAFIGLVGGFLASCLIFGMFYLAFQIEENTRRGGEFFEALGRRMQAREQAA
                     AAVSEPSLPG"
     gene            complement(3553..4491)
                     /locus_tag="PK812_04950"
     CDS             complement(3553..4491)
                     /locus_tag="PK812_04950"
                     /inference="COORDINATES: protein motif:HMM:NF025878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRJ68937.1"
                     /translation="MIVSASTECFHPNERPARWADAVRANFATVDVAPAGTAPIFGAF
                     QLVANEGFGVARFSSRSFSYRRSVAAPDEDESSSLLLDIQQSGTSIMHQCERETCLAP
                     GQAVLYNPGRPLENHFTDQRISTVTILKVPKDRVREEVGAVDPYLCQPLPARTALART
                     LQDEIASALKTPSADGTQTVSNYAMPLLRLLLRDESGLTRQQLFPLVERYLRAHGLDQ
                     PVSPTRLASIFRVSERTVFRIFSERGTTLERSIILLRCQRLAGHLRDATQSGATIASL
                     AAACGFSDAAHASRCFRACFGVTPSEFRHAGLPVAG"
     gene            4632..6620
                     /locus_tag="PK812_04955"
     CDS             4632..6620
                     /locus_tag="PK812_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HRJ68938.1"
                     /translation="MSACCTPAGAAKAAPHPLDPLTEAEIAAACSLLRRKKRLGPDLR
                     FGFVQLEEPAKSVVLGWKAGQPVSRSARLIAFNCKTGATYVAVVCLDDQSVTSFAERP
                     VKQHPYGQPPVIIEEFFKVADIVKSDKGWLKAMKRRGLTKKDIDLVQVDPFSAGYYDR
                     EVEKGRRLVSAVCYWRGHEKDNGYAHPIEGVVALVDLIEGKVVQLVDEEEIVPIPKTK
                     RNYDRASYPKTRKDLKPLDLVQDEGPSFTVEGWNVSWQNWTFRVGFTPREGLVLHQVS
                     YTDKGRTRPIIYRASVTEMVVPYADPTANHFWKSAFDAGEYGLGKLANALELGCDCLG
                     QIHYFDVAAADDAGNPFVMKNAICMHEEDYGILWKHYEFRNETYEVRRSRRLVISFFA
                     TVGNYDYGFYWYLYQDGTIQLEAKLSGIIQTAAVAPGKAYPWGGMVAEGLGGPTHQHF
                     FNVRLHMMVDGEKNTVTEHEFVPRPWGKDNPYGNVFDTTSRVLSRERDAAREADGRTG
                     RYWKIVNSNVRNSVGNPTGYKLVVNPSPLMLAQEGCHMRARGAFATKHVWVTPFDPTQ
                     RYASGEFPNQHAGGDGLPKFIEDNRSIENEDIVLWHSFGHTHVCKPEDFPVMPVEYAG
                     FMLKPNGFFSENAAMDLPPDRNSASKDQRDDDCCKPGP"
     gene            6731..8308
                     /locus_tag="PK812_04960"
     CDS             6731..8308
                     /locus_tag="PK812_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRJ68939.1"
                     /translation="MKPFKHALLAAAGLAGLTLATPALSQTTLRAVMHSDLKILDPIW
                     TTAYIVRNHGYMIYDTLFAQDEKGEIKLQMLEKYDVSADKKVYTFTLRDGLMWHDGQP
                     VTAEDCVASIKRWASRDALGQKMNSFVESMTAKDAKTFTINLKEPTGLVLLGLGKPSS
                     NVPFMMPKRVAETDPNKQIEDFTGSGPFVFKKDEWKPGDKTVYVKFDKYKPRSEPASG
                     LSGGKVAKVDRVEWRAISDQQQAVNALTKGEIDFIEQPSHDLLGGLKKDANVQIIESP
                     QLKGQYTFRPNHLQKPFDNPKVRQALWYAFNQEDFLMANIGDAAYIKECKAMFVCDTP
                     FATTKGMDGLLTSNVKKAQELLKEAGYDGTPIVLMHSTDLKILTNLAPVAKSLMEKAG
                     FKVDMQSMDWQTLVARRTKKDPANAGGWHAFLTAWVAADVMNPVSRAYLNSACEKSLF
                     GWPCDAQMEKLRDDFAREPDPAKQKAIAEAVQVRAIELTPEIPLGEYLQPVAMRKSVK
                     GFLIAPVPVFWNMEVAK"
     gene            8373..9314
                     /locus_tag="PK812_04965"
     CDS             8373..9314
                     /locus_tag="PK812_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ68940.1"
                     /translation="MFGYILRRLIGTIPVMLVVAVFIFLMLRLTPSDPAAIIAGDNAN
                     AEQVAKIRQQLGLDQPMLTQFFIWFGKVLTGDFGESFFFKKTVAALIAERIEPTLSLS
                     LFTIVLAVLIAVPLGVLAAHRQGTWVDRLVMGFAVLGFSVPAFVIGYIFIYVFAIWLN
                     WLPVQGYQRISEGLGGWALRLVLPALTLSVIYVALIARMTRTSVLEVLSEDYVRTARA
                     KGQVERKVMFRHALRNAAVPIVTVIGLGVALLIGGVVVTESLYTIPGLGRLTVDAVLA
                     RDYPTIQAVILLFSFVYVMINLAVDLIYTLLDPRIRY"
     gene            9374..10231
                     /locus_tag="PK812_04970"
     CDS             9374..10231
                     /locus_tag="PK812_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ68941.1"
                     /translation="MALRHPGVIVGGIVVLVVVLMGLLAPLLGTIDPSSINPAARNRV
                     PGAEFTMRTDTGERIRMTALFGTDSLGRDIYSRTIYGARVSLLVGVAVASLSVLIGLA
                     IGLVAGFFRWLDAVVMRIMDGLMAIPAILLAIAMVSLFRAGVLTVILAIVVPEIPRVV
                     RLVRSIVLSVREEPYVEAAVTLGTPTGKLLWRHVLPNTIAPLIVQGTFICASAILVEA
                     ILSFLGIGVPPEVPTWGNIMAEGRQVFSLYPHNIIYPGIFLAVTILAVNVLGDGLRDT
                     LDPKMAKRV"
     gene            10233..11858
                     /locus_tag="PK812_04975"
     CDS             10233..11858
                     /locus_tag="PK812_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ68942.1"
                     /translation="MSMPVLEVSNLSVAIRGLGDRTHAIKGIDLTVGANEIVCVVGES
                     GSGKSVTAQSVMGLLPKGLMKVESGSIRLQGDELTTKTDAQLRAIRGERMAMVFQEPM
                     TALNPVERVGDQIAEVLEIHTDLDAKGRRARVLDIMRAVHLPDPEHMIDAYPHQLSGG
                     QRQRIMIAAALVLDPALLIADEPTTALDVTTQAQILKLIREMQERKKTGVLFITHDFG
                     VVSEIADRVVVMQMGSIVEQGPRDEVLRRPREAYTKMLMASVPSMTPPKRPVVTGAVT
                     LEAQGLFKTYGKRSFWQPKARVVNAVNDVSLTVRRGETLGIVGESGSGKSTVARCVAR
                     LIDPTAGAVKIEGTDIAHLKEAEFRPVRRRVQIVFQDPYRSLNPRRTVGEAIIEGPMN
                     YGLSRHEAIQRARDLMTTVHLSPDALDRYPHQFSGGQRQRICIARALAMEPVLLIGDE
                     PVSALDVSVQAQVLKLLDEVRRKFDLAVLFITHDLRVAAQVCDRIAVMQKGVIVEQGT
                     TVEVFANPQHAYTRALFDAAPGRHQEFGSGATA"
     gene            11884..13335
                     /locus_tag="PK812_04980"
     CDS             11884..13335
                     /locus_tag="PK812_04980"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HRJ68943.1"
                     /translation="MKVKEYAKFDALGLAELVRRRQVTPKELALTAAEAIAKANGEVN
                     CVVETYADRIDGLDETSLGTGPFRGVPFLMKDVFGHEKGRKIEFGSRLCKGMVGQVDS
                     NLTVLLRKAGVNILGRSSAPEYSMAGTSEGLLYGNASNPWKKGYSTGGSSGGAQAAVT
                     SGMVPIAHGSDIGGSIRIPASYSGGVGLKPSRMRISVGPTVDEGGFGYSMNHVQTMTV
                     RDSAAMLDCLAHPMPGDPFVIPKPRLSYATLIKRAPKKLRVGIVLDPLLGVAVDPEVE
                     KAVRKTGKALAGMGHEVEQASVSLGGVDLLEVIRDLFFFDFENRLNGYAKLSGHKPGP
                     DTLEPVIWSIYQRAKEVTPSRFMAAWSRANVVRRTLGQFWTKYDLWLSPTTATTAPPW
                     GTINLSLPGADFDSFSNRVFALPVQYTLPHNVMGTPAMSLPLAMHSNGLPIGVQIAAA
                     PSLDHIVLQVAAALEQEMPWARRAAPPLHVSRF"
     gene            complement(13367..13936)
                     /locus_tag="PK812_04985"
     CDS             complement(13367..13936)
                     /locus_tag="PK812_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HRJ68944.1"
                     /translation="MSGMPDAGNAGNKWVALMIAALALFLAFAEAGNSNNENLAIDSN
                     IEASNLWAFFQAKTIRGTVLRTAVEQMELDLAATSDSAAQDRMKKRIDSWKATIARYD
                     SEPETNEGRKELAARAKAKEETRTLAAQKKEIFELAAKLFQIGIVLASAMIITGMAWL
                     AAAGGLMGIGGAILMGFALWAPNAIHLPF"
BASE COUNT         2450 a         4409 c         4649 g         2446 t
ORIGIN      
        1 aaacaacatg tcggtcgatc aggtctccgg atcgggcaag cgtgggcagg tcctgaaggg
       61 agatgtgctg gaggcgatcg ccaagggtgc ggcgtcgcag ccggcggaaa cgccgaaggt
      121 cgccgaagcg ccgaagtctc cccgtgcgcc ttccgccggt gacgacgcgg cgcgcgagga
      181 gcgggtgcgc atgaccaagc tgcgccagac gatcgcgcgc cgcctcaagg acgcccagaa
      241 caccgctgcc atgctgacga ccttcaacga ggtcgacatg accaacgtca tggcgctgcg
      301 cggccagtac aaggacctgt tcgagaagaa gcacggcgtg aagctcggct tcatgggctt
      361 cttcaccaag gcggtggtgc aggcgctgaa ggagctgccg ggcgtcaatg ccgagatcga
      421 cggcaccgat gtcgtctaca agaactacta ccacgtcggc atcgcggtcg gcacggacaa
      481 gggccttgtc gtgccggtgg tgcgcgacgc ggaccggctc ggcatcgccg gcatcgagaa
      541 aaccatcgcc gatttcggca agcgcgcccg cgacgggcag ctcaagatgg aggagatgac
      601 gggcggcacc ttcaccatca ccaacggcgg catctacggc tcgctgatgt cgacgccgat
      661 cctcaacgcg ccgcaatccg gcatcctcgg catgcacaag atccaggacc ggccgatggt
      721 cgtcggcggc aagatcgaaa tccgcccgat gatgtatctg gcgctctcct acgatcaccg
      781 gatcgtcgac ggcaaggaag ccgtgacctt cctcgtgcgg gtgaaggaaa gcctggaaga
      841 cccggcgcgt ctggtgatgg atctgtgaat gggttcaggg cacagtctcg tgtcctcgaa
      901 ccggtagccg gaggctctca gtcatgacca aagtcgccat cgtcaccggc ggatcgcgcg
      961 gcatcggccg ggcctgctgc ctccggctcg cggacgcggg ctatgcggtg gtggtcaact
     1021 acaccagcgc gaaggccgcc gccgatgagg tcgtggcgga gattgccgcg aagggcggca
     1081 aggccgtggc ggtcaagggg gacgtggcga gtgaggccga cgtgctggcg atcttcgccg
     1141 ccgccgaccg tctcggaccg ctcgccgcgc tcgtcaacaa tgccggcgtc gtcgatgtga
     1201 tgacgcgcgt cgatcaggtc tccgtcacgc ggctggagcg gatgttttcc atcaacgtga
     1261 tcggctcgtt cctgtgcgcc cgcgaggcgg tgaagcgcat gtcaacgaag cacggcggca
     1321 acggcggcgg catcgtcaac atctcgtcgg tggcggccga gcttggctct cccggctggt
     1381 atgtggatta cgccgcctcc aagggcgcga tcgacacgat gaccaagggc ctcggccttg
     1441 aagtcgcggc ggaaggcatc cgcgtcaacg gcgtccggcc ggggatcatc gctaccgata
     1501 tccacgcctc cggcggcatg ccggaccgcg tggcgcagat gcggccgctg ctgccgatga
     1561 agcgcgaagg ctccgccgac gaggtggcgc aggtggtgct ctggctgctg tcggacgcgg
     1621 cctcctacat caccgccacc acgatcaacg tttccggcgg ccgctgagcc gcgcgtcagg
     1681 ggaagttctc atgtcctacg atctcatcgt catcggcacc ggccccggcg gctatgtctg
     1741 cgcgattcgc gcggcgcaac tcggtctgaa gacggccgtc atcgagaagc gcaagactca
     1801 tggcggcacc tgcctcaatg tgggctgcat cccgtcgaag gcgctgctgc atgcctccga
     1861 attgttcgac gaggctggcc atggctttgc cgccttcggc atcgacgtgc cgaagccgaa
     1921 gctcaacctc gcgcagatga tgaagcacaa gcaggagacg atcgacgcca acgtcacggg
     1981 cgtcggcttc ctgctcaaga agaacaaggt gacggcctat cacggcctcg gcaccattct
     2041 ggcgccgggc aaggtccagg tgaccgccga caacggcgcg agcgaaatcc ttgagaccaa
     2101 agcgatcgtc atcgccaccg ggtcggaagt catgccgctg ccgggcgtcg agatcgacga
     2161 gaagcagatc gtctcctcca ccggcgcact ggacctgcca aaggttccag gcaagctcgt
     2221 ggtcatcggc gccggcgtga tcggccttga actcggttcg gtgtggcggc gccttggcgc
     2281 ggacgtcgag gtcatcgagt tcctcgaccg catcctgccg ggcatggacg gggaagcctc
     2341 aaagcaattc cagcgcatgc tggaaaagca gggcatcgcc ttccgcctcg gcgcgaaggt
     2401 caccggcgtc gacaagatca agggcgggct gaaggtgaag gtcgagccgg ccaagggcgg
     2461 cgaagcctcc acgatcggcg cggatgtggt gctggtcgcc atcggccggc ggccattcac
     2521 gcaaggtctg ggacttgaga acgccggcgt cgcgctcgac gcgcgggggc gcatcgtcac
     2581 cgacagtcat tacgccacca acgtgccggg catctatgcc atcggcgatg tgatcgcggg
     2641 gccgatgctg gcccacaagg ccgaagacga aggcgtcgcc ctggccgaaa ttcttgccgg
     2701 caaggcgggc catgtgaact acggggtcat acctggcgtg gtctacactt tcccggaggt
     2761 cgcttccgtg ggcaagaccg aggaggagct gaaggccgag ggtgttgcct atgccgtcgg
     2821 caagttcccc ttcacggcga acggccgtgc acgcgccaac cgcacgacgg acgggttcgt
     2881 gaaagtgctc gccgacaagg ccagcgaccg ggtgctcggc gtgcatatcg tcggcgcggg
     2941 cgcgggcgaa atgatccatg aggcggcggt gctgatggaa ttcggcggtt cgtccgagga
     3001 cctcgcgcgc acctgccacg cgcatcccac gatgtcggag gcggtgaagg aagcggcgct
     3061 cgcggtggaa aagcgcgcga ttcacatgtg atcttgcgcg gcttcctgcc gcgattctga
     3121 cttttggggc ttgcccgacg cctccgcgcg gctcaatctg ccggcttcgt cctgaagcgc
     3181 ggggggttgg catgcgtcgg ttcgttgtat cctgtctcaa gatcattctt gacattggct
     3241 tttggggcgt cgtgcttggc tccatggctg cgggctacat gctgggcgtc agcaattcgg
     3301 ctgccgcaat catgctggga gacggattgt gggcaggcct gctcggcgcc ttcatcgggc
     3361 ttgtcggcgg gtttctggcc tcgtgcctga tcttcggcat gttctatctt gcgttccaga
     3421 tcgaggagaa cacccggcgc ggcggcgagt tcttcgaggc actcggccgg cgcatgcagg
     3481 cgcgcgagca ggcggcggcg gccgtcagcg aaccgtcgct gccgggctga tcaggcaaag
     3541 gcgaggctgg attcagccgg caaccggcag gccggcgtgg cggaattcgc tcggcgtgac
     3601 accgaagcag gcacggaagc agcgggaggc atgcgccgcg tccgaaaatc cgcaggccgc
     3661 cgcgaggctg gcgatggttg cgccggactg ggtggcatcg cgcaggtgtc cggccagccg
     3721 ctggcaccga agcaggatga tcgagcgctc cagcgtcgtg cccctttcgg aaaagatccg
     3781 gaacactgtc cgttcggaca cgcggaagat cgaggcgagg cgcgttggcg aaaccggttg
     3841 gtcgaggcca tgcgcgcgca gatagcgctc gaccagcgga aagagctgct gtctcgtaag
     3901 gccggattcg tcccgcagga gcaggcgcag cagcggcatg gcgtaatttg aaaccgtctg
     3961 cgtcccgtcc gccgacgggg tcttcagcgc cgaggcgatc tcgtcctgaa gggtgcgcgc
     4021 cagtgccgtt cgggccggaa gcggctgaca caagtaggga tccacggctc ccacttcctc
     4081 gcgcacgcgg tccttcggaa ccttgaggat cgtgacggtt gatatccgct ggtccgtgaa
     4141 gtggttttcg agcggccggc cgggattgta gagcacggcc tgaccgggtg cgaggcaggt
     4201 ttcgcgctcg cattggtgca tgatgctggt tccggactgc tggatgtcca gaagcaggga
     4261 ggacgattcg tcctcgtccg gtgcagcaac ggaccggcgg tagctgaacg agcgggagga
     4321 aaaccgcgca acgccaaacc cttcgtttgc gaccaactgg aaggcgccaa agatcggggc
     4381 cgtgccggcc ggcgcaacgt cgaccgtggc gaaattcgcg cggaccgcat ccgcccatcg
     4441 ggccggccgc tcgttcgggt gaaagcactc tgtcgacgca gaaacgatca cggcagccgt
     4501 tctccctgcg gtgagactat cttcggcccg gcgcgccgtc acattgcccg agctggcagc
     4561 cacgttcaag acagccgcgt ttcctgaatg gtagcgctgg gcaaaacgag acaaatccgg
     4621 aggggaccca catgagcgcc tgctgcacgc cagccggggc ggcgaaagcc gcgccgcacc
     4681 cgcttgatcc gctgaccgag gctgaaatcg ccgccgcctg cagcctgctg cgccggaaga
     4741 agcggctggg ccccgatctg cgcttcggct tcgtgcagct ggaagaaccg gccaagtcgg
     4801 ttgtccttgg ctggaaggcg ggacagcccg tcagccggtc ggcccggctg atcgccttca
     4861 actgcaagac cggcgcaacc tatgtcgcgg tcgtctgtct cgacgaccag tcggtgacgt
     4921 ccttcgccga acgtccggtg aagcagcacc cctacggcca gccgccggtg atcatcgagg
     4981 agttcttcaa ggtcgccgac atcgtcaagt ccgacaaggg gtggctgaag gccatgaagc
     5041 ggcgcggcct gaccaagaag gacatcgacc tagtgcaggt cgatccgttt tccgccggct
     5101 actatgaccg ggaggtggag aagggacgcc gtctggtcag cgccgtctgc tactggcgcg
     5161 gccacgagaa ggacaacggc tatgcgcacc cgatcgaagg cgtcgtggcg ctcgtcgatc
     5221 tgatcgaggg caaggtggtc cagcttgtcg acgaggagga gatcgtcccg atcccgaaga
     5281 ccaagcgcaa ttacgatcgc gcctcgtatc ccaagacccg caaggacctg aagccgctcg
     5341 atctggtgca ggacgagggg ccgagcttca cggtcgaagg ctggaacgta tcctggcaga
     5401 actggacctt ccgggtcggt ttcacgccgc gcgaagggct ggtgctgcac caggtgtcct
     5461 acaccgacaa ggggcggacg cggccgatca tctaccgcgc ctcggtcacc gaaatggtgg
     5521 tgccctacgc cgatccgacc gccaaccatt tctggaaaag cgccttcgat gccggcgagt
     5581 acggtctggg caagcttgcc aacgcgctgg aactgggctg tgactgcctc ggccagatcc
     5641 attatttcga cgtggcggcg gcggacgacg ccggcaatcc cttcgtcatg aagaatgcga
     5701 tctgcatgca cgaggaggac tacggcatcc tgtggaagca ctacgagttc cgcaacgaga
     5761 cctatgaggt gcgccgctcg cgccggctgg tgatctcgtt cttcgcgacc gtcgggaact
     5821 atgattacgg cttctactgg tatctctatc aggacggcac gatccagctc gaggccaagc
     5881 tctccggcat catccagacc gccgcggtcg cgccgggcaa ggcctatccc tggggcggca
     5941 tggtggccga gggcctcggc ggtccgacgc accagcattt cttcaacgtg cgcctgcaca
     6001 tgatggtcga cggggagaag aacacggtca ccgaacacga gttcgtgccg cgcccctggg
     6061 ggaaggacaa cccctatggc aatgtgttcg acaccacatc gcgcgtgctg tcgcgggagc
     6121 gggacgcggc ccgcgaggcg gatgggcgca ccgggcgcta ctggaagatc gtcaacagca
     6181 acgtccgcaa cagcgtcggc aatccgacgg gctacaagct cgtggtgaac ccttccccgc
     6241 tgatgctggc gcaggagggg tgccacatgc gggcgcgcgg cgccttcgcc accaagcatg
     6301 tctgggtcac gcccttcgat ccgacccagc gctatgcctc gggcgagttt cccaaccagc
     6361 acgccggcgg cgacgggctg ccgaaattca tcgaggacaa tcgttcgatc gagaacgagg
     6421 atatcgtgct ctggcattcc ttcgggcata cccatgtctg caagcccgag gattttccag
     6481 tcatgccggt cgagtatgcc gggttcatgc tgaagccgaa cgggttcttc tcggagaatg
     6541 cggcgatgga tctgccgccg gatcgcaatt ccgcttcaaa ggaccagcgc gacgacgact
     6601 gctgcaagcc gggaccctga caactgcagg atatttcagt tgtgcgtcgg cctggcttca
     6661 ggttttatga atgacacaag acttggccgg agcaaccggc agcacaccat gggtgaggga
     6721 ggatatcgcc atgaaaccgt tcaagcacgc cctgcttgcc gccgccggcc ttgcggggct
     6781 gacgttggcc acgccggccc tgtcgcagac gacgctcagg gccgtgatgc actcggatct
     6841 gaagatcctc gatccgatct ggaccacggc ctatatcgtc cgcaaccacg gctacatgat
     6901 ctacgacacc cttttcgcac aggacgagaa gggcgagatc aagctgcaga tgctggagaa
     6961 gtatgacgtt tccgccgaca agaaggtcta taccttcacg ctgcgggacg ggctgatgtg
     7021 gcatgacggc cagccggtca cggcggagga ctgcgtcgcc tcgatcaagc gctgggcgtc
     7081 gcgcgacgcg ctcgggcaga agatgaactc ctttgtcgag tccatgaccg ccaaggatgc
     7141 caagaccttc accatcaacc tgaaggagcc gacaggcctg gttctgctcg gcctcggcaa
     7201 gccgtcgtcg aacgtgccct tcatgatgcc gaagcgggtg gcggagaccg acccgaacaa
     7261 gcagatcgag gacttcaccg gatcgggtcc gttcgtgttc aagaaggatg aatggaagcc
     7321 cggcgacaag acggtctacg tcaagttcga caagtacaag ccgcgttcgg agccagcgtc
     7381 cggcctttcg ggcggcaagg tcgccaaggt ggaccgcgtg gaatggcgcg ccatctccga
     7441 ccagcagcag gcggtcaacg ccctgacgaa gggagagatc gacttcatcg agcagccgag
     7501 ccacgatctg ctgggcggac tgaagaagga cgccaacgtt cagatcattg aatcgccgca
     7561 gctgaagggg cagtacacct tccggccgaa ccatctgcag aagccgttcg acaatccgaa
     7621 agtgcggcag gccttgtggt acgcgttcaa tcaggaagac ttcctgatgg cgaacatcgg
     7681 cgatgcggcc tacatcaagg aatgcaaggc catgttcgtc tgcgacacgc cgttcgcgac
     7741 gaccaagggg atggacgggc tgctcacctc gaacgtgaag aaggcgcagg agcttctcaa
     7801 ggaagctggc tatgacggca cgccgatcgt gctgatgcat tcgaccgacc tcaagatcct
     7861 tacgaacctg gcgccggtcg cgaaatcact gatggagaag gccggcttca aggtcgacat
     7921 gcagtcgatg gactggcaga cgcttgtcgc ccgtcgcacc aagaaggatc cggcgaacgc
     7981 cggcggctgg catgcgttcc tgacagcctg ggttgccgcc gacgtcatga atccggtgag
     8041 ccgggcctat ctcaattcag cttgcgagaa gtctctgttc ggctggccct gcgatgccca
     8101 gatggaaaag ctgcgcgatg atttcgcgcg cgaacccgat ccggccaagc agaaggcgat
     8161 cgcggaagcg gtgcaggtgc gcgccattga actgacgccg gaaatcccgc tcggcgagta
     8221 cctgcagccc gttgccatgc gcaagagcgt caaaggcttc ctgatcgcgc cggtacccgt
     8281 gttctggaac atggaagtcg ccaagtgatc ctggaccgcc gcccgggcgc atccgcaccc
     8341 gggcggcatt cctttcggct tttcccgtga ccctgttcgg ctacatcctt cgccgcctca
     8401 tcggcaccat cccggtgatg ctggtcgttg ccgtgttcat cttcctgatg ttgcggctga
     8461 cgccctccga tccggccgcc atcatcgccg gcgacaatgc caacgccgag caggtggcga
     8521 aaattcgcca gcagctcggc cttgaccagc cgatgctgac gcagttcttc atctggttcg
     8581 gcaaggtgct gaccggtgat ttcggcgaga gcttcttctt caagaagact gtcgccgcgc
     8641 tgattgccga gcggatcgag ccgacgctgt cgttgtcgct cttcaccatc gtgctggcgg
     8701 tgttgatcgc ggtgccgctc ggcgtcctcg ctgcgcaccg gcagggaacc tgggtcgacc
     8761 gcctggtcat gggctttgcc gttctcggct tttcggttcc ggccttcgtc attggctaca
     8821 tcttcatcta tgttttcgcg atatggctga actggctccc ggttcagggc taccagcgca
     8881 tcagcgaggg acttggcggc tgggcgctca ggctcgtgct gccggcattg acgctctcgg
     8941 tcatctacgt tgcgctgatc gcccgcatga cccgcaccag cgtgctcgaa gtgctgtcgg
     9001 aagactatgt ccggacggcg cgtgccaagg ggcaggtcga gcgcaaggtg atgtttcgtc
     9061 atgccttgcg caatgcggcg gtaccgatcg tgaccgtcat cggtcttggc gtggcgctgc
     9121 tgattggtgg cgtcgtggtc accgagagcc tctacaccat acccggcctg ggccggctga
     9181 cggtcgatgc cgtgctggcg cgggactatc ccaccattca ggccgtgatc cttctgttct
     9241 ccttcgtcta tgtcatgatc aacctcgcgg tggacctcat ctacaccctg ctcgatccga
     9301 ggatccgtta ctgatggcgg ccggcgccac ttccgcgccg cagggcgttt ccaggccgcc
     9361 ttcggccgtg gccatggccc tgcggcatcc cggcgtcatc gtcggtggga tcgtggtgct
     9421 cgtcgtcgtg ctgatgggcc ttctggcgcc gctgcttgga accatcgatc cgtccagcat
     9481 caatcccgcc gcccgcaacc gggtgccggg cgccgaattc accatgcgca ccgacaccgg
     9541 cgaacgcatc cgcatgacag cgctgttcgg gaccgacagc ctggggcgcg acatctacag
     9601 ccggacgatc tacggcgcgc gcgtctcgct gctggttggc gtggccgtgg ccagcttgag
     9661 cgtgctgata ggcttggcga tcggcctcgt cgccgggttc ttccgctggc tcgatgcggt
     9721 ggtgatgcgg atcatggacg ggttgatggc gatcccggcc atcctgctgg ccatcgcgat
     9781 ggtctcgctg ttccgggccg gcgtgctgac ggtcatcctg gcgattgtcg tgccggagat
     9841 cccgcgcgtc gtgcgactcg tgcggtccat cgtgctcagc gttcgcgagg agccctatgt
     9901 cgaggcagcc gtgacgctcg ggacgccgac gggcaagctg ttgtggcgcc atgtgctccc
     9961 caatacgatc gcgccgctca tcgtgcaggg caccttcatc tgcgcctcgg cgatcctggt
    10021 cgaggccatc ctgtccttcc tcggcatcgg cgttccaccg gaggtgccga cctggggcaa
    10081 tatcatggcg gaggggcggc aggtgttcag cctctacccc cacaacatca tctatcccgg
    10141 cattttcctg gcggtgacca tcctcgcggt caacgtgctg ggcgatggct tgcgcgatac
    10201 gctcgaccca aagatggcga agcgggtctg acatgagcat gcccgttctc gaagtcagca
    10261 acctttcggt cgcgatccgc ggcctcggcg accggacgca tgcgatcaag ggcatcgacc
    10321 tgaccgtcgg cgccaacgag atcgtctgcg tggtcgggga gtccggatcg ggcaagtcgg
    10381 tcacggccca gtcggtcatg gggctgctgc cgaaggggct gatgaaggtc gagagcggtt
    10441 ccatccgcct gcagggcgac gaactgacca ccaagaccga tgcgcagctg cgcgccattc
    10501 gcggcgagcg catggcgatg gtgttccagg agccgatgac cgcgctcaac ccggtggagc
    10561 gggtcggaga ccagatcgcg gaggtcctgg aaatccacac cgacctcgac gcgaagggcc
    10621 gacgcgcccg cgtgctggac atcatgcgcg cggtgcatct gcccgatccc gagcacatga
    10681 tcgatgctta tccgcaccag ctttccggcg ggcagcgcca gagaatcatg attgccgccg
    10741 ccctggtgct cgacccggcg ctgctgatcg ccgacgagcc gacgaccgcg ctggacgtca
    10801 ccacgcaagc gcagatcctc aagctcattc gcgagatgca ggagcgcaag aagacgggcg
    10861 ttctgttcat cacgcatgac ttcggcgtgg tgtcggaaat cgccgaccgc gtcgtcgtca
    10921 tgcagatggg cagcatcgtg gagcaggggc cgcgcgacga ggtgctgcgg cgcccgcgcg
    10981 aggcctatac caagatgctc atggcttcgg tgcccagcat gaccccgccg aagcgcccgg
    11041 tggtcaccgg cgcggtgacg ctcgaagcgc agggcctgtt caagacctac ggaaagcgca
    11101 gcttctggca acccaaggcg cgcgtggtca acgccgtcaa cgatgtctcc ctcaccgtgc
    11161 ggcgcggcga gacgctgggg atcgtcggcg agtcgggctc cggcaagtcg accgtggcgc
    11221 gctgcgtggc gcggctgatc gatccgacag cgggtgcggt caagatcgag ggaaccgaca
    11281 tcgcccatct gaaggaagcg gagttccgtc ccgtgcggcg ccgggtgcag atcgtgttcc
    11341 aggaccccta tcgctcgctc aatccgcgcc ggaccgtcgg cgaggcgatc atcgaagggc
    11401 cgatgaacta cgggctgtcg cgccacgagg cgatacagcg cgcccgcgac ctgatgacga
    11461 cggtgcacct gtcgccggat gcgctcgacc gctacccgca ccagttctcc ggagggcagc
    11521 gtcagcgcat ctgcattgcg cgtgccctgg cgatggagcc tgtcctgctg atcggcgacg
    11581 aacctgtgtc cgcgcttgac gtttccgtgc aggcccaggt gctgaagctg ctggacgagg
    11641 tgcgccgcaa gttcgatctc gccgtgctgt tcatcacgca cgatctcagg gtggcggcgc
    11701 aggtctgcga ccgcatcgcg gtgatgcaga agggcgtgat tgtcgagcag gggacgaccg
    11761 tcgaggtgtt cgccaatccg caacatgcct atacgcgcgc cctgttcgat gccgcgcccg
    11821 gccggcacca ggaattcggc tccggcgcca ccgcctaggg tcgcaccatc gaggaggcct
    11881 gtcatgaagg tcaaggaata cgcgaagttc gatgccctgg gcctggccga gctggtgcgc
    11941 cgccggcaag tgacgccgaa ggaactggcg ttgacggccg ccgaagccat cgccaaggcc
    12001 aacggtgagg tcaattgcgt ggtcgagacc tacgccgacc ggattgacgg gctggacgag
    12061 acatcgctcg gcaccggccc gttccggggc gtgcccttcc tgatgaagga cgtgttcggg
    12121 catgaaaagg gacgcaagat cgaattcggc tcgcgactct gcaagggcat ggtcgggcag
    12181 gtcgacagca atctcactgt gctgctgcgc aaggccggcg tgaacatcct cggccggtcc
    12241 tcggcgccgg aatattcgat ggcggggacg agcgaaggcc tgctctacgg caatgcctcg
    12301 aatccctgga agaagggcta ttccaccggc ggttcctcgg gcggggcgca ggctgccgtg
    12361 acgtcgggca tggtgcccat cgcccacggt tcggacatcg ggggctcgat ccgcatcccg
    12421 gccagctaca gcggcggcgt gggcctgaag ccgtctcgca tgcgcatctc ggtcggaccg
    12481 acggtcgacg agggcgggtt cggctattcg atgaaccacg tgcagaccat gaccgtgcgc
    12541 gattccgccg cgatgctgga ttgcctcgcc catccgatgc cgggcgatcc gtttgtgatc
    12601 cctaagccgc gcctctccta tgcgacgctg atcaagcgcg cgccgaagaa actccgggtc
    12661 gggatcgtgc tcgatccgct gctgggcgtt gcggtcgatc ccgaggtgga gaaagcggtc
    12721 agaaagaccg gaaaggccct ggccggcatg gggcacgagg tcgagcaggc gagtgtcagc
    12781 ctgggcggcg tcgatctgct ggaagtcatc cgcgacctgt tcttcttcga cttcgaaaac
    12841 cggctcaacg gctatgcgaa gctgtcgggc cacaagcccg ggccggatac gctggagccg
    12901 gtgatctggt cgatctatca gcgcgccaag gaggtcacgc cgtcgcgctt catggcggcc
    12961 tggtcgcggg ccaacgtcgt gcgccggacg cttggccagt tctggacgaa gtacgacctt
    13021 tggctgtcgc cgaccacggc gacgaccgcg ccgccctggg gcacgatcaa cctgtcgttg
    13081 ccgggtgccg acttcgacag tttctcgaac cgggtcttcg ccctgccggt ccagtacacg
    13141 ctgccgcaca acgtgatggg cacgccggcg atgtctcttc cgctggcgat gcattccaac
    13201 ggattgccga tcggcgtgca gatcgcggcc gcgccatcgc tcgatcacat cgtgctgcag
    13261 gtggccgccg cgctggagca ggaaatgccc tgggccaggc gggcggcccc gccattgcac
    13321 gtctcgcggt tctgacagcg cccggcgtcg gccgggcggc gaacccctag aacggcaggt
    13381 gtatggcgtt gggcgcccag agcgcgaatc ccatgaggat cgccccgccg atccccatca
    13441 gcccgccggc tgcggccagc caggccatgc ccgtgatgat catggcggaa gccagcacga
    13501 tgccgatctg gaacagcttg gccgccagtt cgaagatctc cttcttctgg gcagccaacg
    13561 tgcgtgtctc ttccttggcc ttggcccggg ccgcgagttc cttgcggcct tcgttggtct
    13621 cgggttccga atcgtagcgg gcgatcgtcg ctttccagga gtcgatgcgc ttcttcatgc
    13681 ggtcctgtgc ggcgctgtcg cttgtcgcgg ccaggtcgag ttccatctgc tccacagccg
    13741 tgcgcagcac ggtgccgcga atggtcttgg cctggaagaa ggcccacaga ttcgaggcct
    13801 cgatattgga atcgatcgcg agattctcat tgttgctgtt tccggcctcg gcgaaggcaa
    13861 gaaacagcgc cagcgccgcg atcatcagcg cgacccactt gttgcccgca tttcccgcgt
    13921 ccggcatgcc cgacatgatt cgcccctccg ttgg
//
LOCUS       DAOMXN010000038        13917 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_2414_length_13917_cov_12.213461, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000038 DAOMXN010000000
VERSION     DAOMXN010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 13917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13917
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2414_length_13917_cov_12.213461"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /gene="cysQ"
                     /locus_tag="PK812_04990"
     CDS             complement(<1..822)
                     /gene="cysQ"
                     /locus_tag="PK812_04990"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HRJ68945.1"
                     /translation="MVLTRNDLALAALACSAGAAILAVRAAALASDTKPDGSPVTEAD
                     RRAERIILDGIAVIWPGLPVIAEEAVSGGQVPVVADTFVLVDPLDGTREFIAGRAEFT
                     VNIALVRHRVPVCGVVFAPATGAMFLAGEGGGAWRADVDGCRPPGDGAFRRIGVRPCG
                     AGLVALVSHSHGDEMTEAWLAGQPVVARRNIGSSLKFCLIAAGEADVYPRFGPTMEWD
                     TAAGDAVLRAAGGKVLQPDGAPFLYGKAATGFRNGPFIAPGVGHTDRREEDLHLDR"
     gene            complement(824..2902)
                     /locus_tag="PK812_04995"
     CDS             complement(824..2902)
                     /locus_tag="PK812_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HRJ68946.1"
                     /translation="MVQVNPVTTLDLDGEIAVLTLNSPPVNALGQQVRAGLKAGIEQA
                     VANPAVKAIVFICAGRTFSAGADITEFGKPMTEPFLPVVHATFENCPKPVVAAIHGTA
                     LGGGFETALVCHYRIAVPSAKFGLPEIKLGLIPGAGGTQRLPRLAGVEKAMDLIVSGA
                     MFGARDALSMGVVDRLASEDGLLADAVAFAREIVSRGMAPVRVRDRDEKLAAARGKPE
                     IFEAFRKANARKNRGFEAWHVAMTSIGNALTMPFDEALTEERRLFMELIKSPQSIAQR
                     HVFFAEREVAKIPDVPTDTPAIPVQTVGIIGAGTMGGGIAMNFLNAGIPVTIVEAKQD
                     ALDRGVAIIRKNYEATAQKGRMTMADVEKRMALLTPTLDFAALAPSDLVIEAVFENMD
                     IKKEIFRRLDATVKQGAILATNTSYLDVNEIAAATGRPEFVVGMHFFSPANVMRLLEV
                     VRGDKTAKPVVQTAMQIGKKIGKIAVLVGVCHGFVGNRMLAQRQREAQKLILEGAMPW
                     DVDRVLYAFGLPMGPFAMSDLAGLDIGWSKETSTSSTVREILCEMDRRGQKTRAGFYD
                     YDENRTARPSPVVEKIILDLSAKQGIMRRAISDEEILQRCIYPMINEGAKILEEGKAL
                     RASDIDIVWINGYGWPVYRGGPMHYADSLGLKHVLSVMKGFEASHGADFKPSALLERL
                     VAEGKSFARM"
     gene            complement(2942..4087)
                     /locus_tag="PK812_05000"
     CDS             complement(2942..4087)
                     /locus_tag="PK812_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRJ68947.1"
                     /translation="MDFDLNEEQQLLKDSVDRLFASRNDFETRRKIQASDAGWSRENW
                     GAYAEMGLLGLPFSEADGGFDAGPVSVMIVMEAIGRSLALEPYLSTVVLAGSVLRRFG
                     NAAHRAEYIPRIIAGDMTATLAFAEPQSRYDLHDVALAARKDGASYVLDGRKGLVLHG
                     GSADRLIVTARTAGGRRDHKGISVFVVDARAEGVSRRSYPTQDGGRAAEVELSGVRVP
                     AEDLVGTLDEGLAIITRAAEDGIAALCAEAVGLMADMQAMTVDYLKTRKQFGTTIGSF
                     QALQHRASDMVVELEQARSMMLYATMNVDEPDAMARATALSAAKLQIGKAIRFIGQQS
                     IQMHGGIGMTMEYKVGHLFKRATMIDLMFGDSDHHLEKLAASDAAIV"
     gene            complement(4100..5299)
                     /gene="pimC"
                     /locus_tag="PK812_05005"
     CDS             complement(4100..5299)
                     /gene="pimC"
                     /locus_tag="PK812_05005"
                     /EC_number="1.3.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018515 - pimeloyl-CoA dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-CoA dehydrogenase large subunit"
                     /protein_id="HRJ68948.1"
                     /translation="MDLRLTPEELAFRAEVRAFFDESIPQDIRAKLTEGRHPDKADMV
                     RWQKILHARGWSVPHWPVEWGGTGWDAVKLYIFRDEMQRTPAPEPLPFGVSMVGPVIY
                     TFGSQEQKTRFLPRIASMDDWWCQGFSEPGAGSDLAGLKTTARRDGDHYVINGQKTWT
                     TLAQYADWIFVLARTSPEAKKQEGISFLLVDMKTPGITVRPIQTIDGGHEVNETFFDD
                     VRVPVENLVGVENRGWDYAKFLLGNERTGIARIGITKARLRRVRELAALEQGGGRLID
                     SARFRQKLAAVEVELKALEMTQLRVVAGEARRERGKPDPASSVLKIRGSELQQSVSEL
                     LLEVAGPYALPYRDEHDPEGWNEPAIGPDWAAMLAPNYFNLRKVSIYGGSNEIQRSII
                     SKAILGL"
     gene            complement(5486..6118)
                     /locus_tag="PK812_05010"
     CDS             complement(5486..6118)
                     /locus_tag="PK812_05010"
                     /inference="COORDINATES: protein motif:HMM:NF018264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrsF family protein"
                     /protein_id="HRJ68949.1"
                     /translation="MKTDDLVRLLAADNAGATENAERQSWLSGASLTAGLAVSLMLFW
                     AGVGLRADIGEAFFAIVRKEAVTLAIAAAGAVALQRLSAPEGRGFFGWMAPLALALFF
                     VGGELASQGSEGWQARMIGRNGLVCLTTIPALSLPLLVGLLLALRRRAPGNAALAGAA
                     SGLAAVGIGASLYALHCTDDSTLFVAFWYSIAAVAMTVVGAVAGRQVLRW"
     gene            complement(6115..6630)
                     /locus_tag="PK812_05015"
     CDS             complement(6115..6630)
                     /locus_tag="PK812_05015"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRJ68950.1"
                     /translation="MRAALGGDDSAYRTLLGELARAFRSFVRARLARQGRGNADIEDI
                     VQEALMAIHLKRGTWDQSRPFSIWAQAVLAHKIADRLRAQSVRMHENVDALADHLPAE
                     ESDSSAASDLHRVLSQLDQNSRRIVTAMSVEGRSAAEVASELGSSEGAVRVALHRALA
                     RLSAMYRGLPR"
     gene            6844..7125
                     /locus_tag="PK812_05020"
     CDS             6844..7125
                     /locus_tag="PK812_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020047390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HRJ68951.1"
                     /translation="MDRRTLSLALAASLTSAFAMAPATAVAQEKEKCFGIALKGKNDC
                     AAGPGTTCAGTSKTDFQGNAWSYVPKGTCEKTASKTSPTKFGMLKEFKS"
     gene            7158..8057
                     /locus_tag="PK812_05025"
     CDS             7158..8057
                     /locus_tag="PK812_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014130684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HRJ68952.1"
                     /translation="MLKTPEASSSGLARSALSSLPRRAGIGFKPQHFEELSERGSRTG
                     FLEVHAENYMGAGGPALAQLERLRRDYPLSLHGVALSIGGEGPLNLEHLTRLRILLER
                     FQPSAFSEHLAWSSHGPYYFNDLLPVAYDGPTLDRVCEHVDLAQNTLGRRLLLENPST
                     YLQFSESSIDEVAFISEVARRTGCGLLLDVNNVAVSCANHGRDPFAYLDAFPVACVGE
                     IHLAGYAEEHDTLGAPLRIDAHGSPVEPDVWALFRHILNRTGPIATLIEWDNDVPDLA
                     TLLAEAKKADACLAGSGNGCRAA"
     gene            8054..8818
                     /locus_tag="PK812_05030"
     CDS             8054..8818
                     /locus_tag="PK812_05030"
                     /inference="COORDINATES: protein motif:HMM:NF021364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HRJ68953.1"
                     /translation="MTASHQSLLAAALLGPDLTIPGLKTWNGSDPVQRFNVYRNNVVH
                     SLVEALRAKFPVVEHLVGRTFFGAMAQHFVRSAPPKSVIMARYGDEFPDFVASYAPAG
                     PLAYLADVARLEAARVRAYHAADARALGPADFETIPPHALADLVLTLHPTVRLIRSAH
                     AIYALWAAHQGELEIGDVDPAVPQDVLVVRPAGEVLVASLPQGAAEILECLASGAQLG
                     AALMASGTPGLEAIPVFRMLVMLGAFCALSLPGEIE"
     gene            8815..9312
                     /locus_tag="PK812_05035"
     CDS             8815..9312
                     /locus_tag="PK812_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HRJ68954.1"
                     /translation="MTAGMFNTAISLFDRIEDAFSRIPHTLIALVGRIAIAGTFWKSG
                     QTKVQGFALDLFEGRFELGWPRFADSTVDLFRDEYKLPLLPPEFAALAATLAEHFFPV
                     LILIGLATRLSAAALLVMTLVIQVFVYPSAWAIHGTWAAVLLWLMSGGAGTISVDHML
                     RKRFG"
     gene            complement(9387..10208)
                     /locus_tag="PK812_05040"
     CDS             complement(9387..10208)
                     /locus_tag="PK812_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68955.1"
                     /translation="MPEAADDGFRQACTRPASTGLSRLRSGPRGFGLCSCSDNRVFYC
                     HELAGSLVRVRMVRRPSQLQKQKHTGLAVVESKWWAGKNTSVSPIFDLISDVFLANPN
                     HYHYEMVSGKRAAKEVIPRIAADPHCSVLYIAAHGEPDGIAWHNGDKLSRLEFRTMLK
                     AIRATRGSQLSGVYFASCRFMTQETADYLFQEAVCPWWIAGYSEQVDWLDSTALDMLV
                     FRSLMSRRKGTDLARIKAVAEDLNSRCAGLIRELGFGIYVRKPGTGGAKNLLASS"
     gene            complement(10213..12834)
                     /gene="clpB"
                     /locus_tag="PK812_05045"
     CDS             complement(10213..12834)
                     /gene="clpB"
                     /locus_tag="PK812_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012454198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HRJ68956.1"
                     /translation="MNFDKYTERARGFVQAAQTIALREGHQQFAPEHLLKALLDDEQG
                     LASGLIERAGGDPRTVRQATESALKRHTRVEGSGAGNIYVTPQLARVFVAAEKAAEKA
                     GDSFVTVERMLLALAVEKDTEAGKALAQGGLTPQALNGAIEAIRKGRTADSATAENAY
                     EALKKYARDLTEAARNGKLDPVIGRDEEIRRAIQVLSRRTKNNPVLIGEPGVGKTAIV
                     EGLALRIVNGDVPESLKDKALLALDMGALIAGAKYRGEFEERLKSVLSEVMAAEGGII
                     LFIDEMHTLVGAGKADGAMDASNLLKPALARGELHCVGATTLDEYRKHVEKDAALARR
                     FQPVFVSEPTVEDTVSILRGLKEKYEQHHGVRITDSALVAAATLSNRYITDRFLPDKA
                     IDLVDEASARLRMQVDSKPEALDSIDREIIRLRIEQEALKKETDAASRDRLAKLQQEL
                     AVLEEQSAALTTSWQAEKNKLGKAADLKKQLEGARNDLAIAQRKGEYQRAGELAYGVI
                     PGIEKQLKAIEEAAGSGGMLEEAVTTDHIAQVVSRWTGVPVDKMLEGEKDKLLRMEAE
                     IGKRVVGQEEAISAVSTAVRRARAGLQDPNRPIGSFMFLGPTGVGKTELTKALAGFLF
                     DDDTAMVRIDMSEFMEKHSVARLIGAPPGYVGYEEGGALTEAVRRRPYQVVLFDEVEK
                     AHPDVFNVLLQVLDDGRLTDGQGRTVDFRNTLIIMTSNLGSEYLVNQGEGEDTDGVRD
                     EVMGVVKGHFRPEFLNRIDEIILFHRLKREHMGRIVDIQMERLRKLLEERKITVDLDA
                     SARGWLAEAGYDPAYGARPLKRAIQKNLQDPLAEMILAGSVKDGETVPVMAGPGGLIV
                     GSAPSQPVRRPKGAALN"
     gene            12996..13760
                     /locus_tag="PK812_05050"
     CDS             12996..13760
                     /locus_tag="PK812_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HRJ68957.1"
                     /translation="MTASVAFLTRYPVKGLSPEPLQSAVLTAGAHFPGDRMFAIENGP
                     SGFDPAAPVHQPKFKYLMLMRNAALARFQTRYDDASGVLTIRRGAETVAQGDLRTEDG
                     RRAIELFYDMELADDMRGPAKVLMAPEGHRFMDSRSGFVSLLNRATLSAIAAKAGRRG
                     LDPMRFRANIGIDGLPPWGEFDLVGKEVRIGEATLHITKRIDRCAATHVDPATAVRDI
                     DMVGLLEREFGHHDCGVYAEVVTGGTIRLGNAVAAS"
BASE COUNT         2438 a         4687 c         4228 g         2564 t
ORIGIN      
        1 tcgatccaag tgaaggtctt cttctcttcg atcggtatgg ccgacgccgg gcgcgatgaa
       61 cgggccgttg cgaaaccccg tcgcagcctt cccgtaaagg aagggcgctc catccggctg
      121 gaggaccttg ccgccggcgg cgcgcagcac ggcatccccc gccgccgtat cccattccat
      181 ggttggaccg aaccttgggt acacgtcggc ttcgcccgct gcgatcaggc agaacttgag
      241 cgaggacccg atgttgcggc gcgcgacgac aggctgaccg gcaagccagg cctcggtcat
      301 ctcgtcaccg tggctgtgcg agaccagcgc gaccagtccg gctccgcaag ggcggacacc
      361 gatacggcga aacgcgccgt cgcccggcgg ccggcaacca tccacatcgg cccgccatgc
      421 gcctccgccc tcgccggcca ggaacatggc gcccgtggcc ggggcgaaga ccacgccgca
      481 gaccggcaca cgatgcctga ccagcgcaat attgacggtg aattcggcgc gaccggcgat
      541 gaactcgcgc gtaccgtcga gcggatcaac cagcacgaag gtatcggcaa ccaccggaac
      601 ctgtccgccg gataccgcct cttccgcgat gacgggcagc ccaggccaga tcactgcaat
      661 gccgtcgagg atgatccgct ctgcccgccg gtcggcctcc gtcaccggag agccatccgg
      721 cttggtgtcg ctggcgagcg cggcggcgcg caccgccaag attgccgcgc cggcactaca
      781 ggccagcgcg gccagcgcga gatcgttacg cgtcagcacc atgtcacatg cgggcgaagc
      841 ttttgccttc tgccacgagg cgttcgagca gggcggacgg cttgaaatcc gcgccgtggc
      901 tggcttcgaa gcctttcatg accgaaagca cgtgcttgag gcccaggctg tccgcgtaat
      961 gcatcggacc accgcgatag accggccagc catagccgtt gatccagacg atatcgatgt
     1021 cggaagcgcg cagcgccttg ccttcttcga ggatcttcgc gccctcgttg atcatgggat
     1081 aaatgcagcg ctgcaggatt tcctcgtcgg aaatcgcgcg gcgcatgatg ccctgcttgg
     1141 ctgacaggtc gaggatgatc ttctccacca ccggcgaggg acgagccgtc cggttttcgt
     1201 cgtagtcata gaatccggcc ctggtcttct gaccacgccg gtccatctcg cagaggattt
     1261 cgcggacggt agaactggtc gaggtttcct ttgaccagcc gatgtcgagg ccagcgagat
     1321 cgctcatggc aaaggggccc atcggcaggc cgaacgcata gagcacgcga tcgacgtccc
     1381 acggcatcgc accctcgagg atcagcttct gggcctcgcg ctggcgctgg gccagcatgc
     1441 ggttgcccac gaaaccgtga cacaccccga ccagaaccgc gatcttgcca atcttcttgc
     1501 cgatctgcat cgcggtctgc acaaccggct tcgccgtctt gtcgccgcgc accacctcaa
     1561 gcagacgcat tacgttggcc ggcgagaaga agtgcatgcc gaccacgaac tccggtcgcc
     1621 cggtcgcggc ggcgatctcg ttgacatcga gataagaggt gttggtggcc agaatcgcgc
     1681 cctgcttcac ggtcgcgtcg agcctgcgga atatctcctt cttgatgtcc atgttttcga
     1741 acaccgcctc gatcaccaga tccgagggag ccagcgcggc gaagtcgagt gtcggcgtga
     1801 gcagcgccat gcgcttttcg acatccgcca tggtcatgcg gcccttctgg gcggtcgcct
     1861 cgtagttctt ccggatgatc gcgacgccac ggtcgagcgc atcctgcttc gcctcgacga
     1921 tggtcacggg aatgccggcg ttgaggaagt tcatggcgat gccgccgccc atcgtgccgg
     1981 caccgatgat gccgaccgtc tgcacgggaa tggccggcgt atcggtcgga acatcgggaa
     2041 tcttcgccac ctcgcgctcg gcaaagaaga catgacgctg ggcgatcgac tgcggcgact
     2101 tgatcagctc catgaacagg cgccgctcct cggtcagcgc ctcgtcgaag ggcatcgtca
     2161 gtgcattccc gatcgacgtc atggccacgt gccaggcttc aaagccgcgg ttcttgcggg
     2221 cgttggcctt gcggaaggcc tcgaagatct ccggcttgcc acgcgctgcg gccagttttt
     2281 catcgcggtc gcgcacacgc acaggcgcca tgcccctgga aacgatctcg cgcgcaaagg
     2341 cgactgcatc tgccagcagg ccgtcctccg acgcgaggcg atcgacgacg cccatggaga
     2401 gtgcatccct ggcgccaaac atcgcgccgg agacaatcag gtccatggcc ttctcgaccc
     2461 ctgccagccg cggcaggcgc tgcgtgccac cagctccggg gatgaggccg agcttgatct
     2521 ccggcaagcc gaacttcgcc gagggaaccg caatgcggta gtggcagacc agcgcggtct
     2581 cgaacccgcc gccgagggcg gtgccatgga tggccgcgac aaccggtttc ggacagtttt
     2641 cgaatgtggc gtgcacgacc ggcaggaacg gctccgtcat gggcttgccg aattcggtga
     2701 tgtcggcgcc cgcgctgaag gtgcgtccag cacagatgaa cacaatcgcc ttcacggccg
     2761 gattggcgac ggcctgctcg attccggcct tgagcccggc ccggacctgc tggccaagcg
     2821 cattcaccgg cggcgagttc agcgtgagca ccgcaatctc gccatcgagg tcgagcgtcg
     2881 tgaccggatt gacctgaacc atgtacgtgt ttcctgggac ttgcatgaag tgagaagcgg
     2941 gtcagacgat tgcagcgtcg gacgcggcga gcttttccag atgatgatcg ctgtcgccga
     3001 acatgagatc aatcatcgtg gcacgcttga acagatgccc gaccttgtac tccatcgtca
     3061 tgccgatgcc gccatgcatc tggatcgact gctgaccgat gaagcggatc gccttgccga
     3121 tctgcagctt cgccgccgac agcgccgtgg cgcgcgccat ggcatcgggc tcgtccacgt
     3181 tcatcgtcgc atagagcatc atgctgcgcg cctgctccag ttcgacgacc atgtcggatg
     3241 cgcggtgctg caacgcctga aacgagccga tggtcgtgcc gaactgcttg cgcgtcttca
     3301 ggtagtccac ggtcatggcc tgcatgtccg ccatgaggcc aaccgcctct gcgcacagcg
     3361 cggcgatccc gtcctccgcg gcccgcgtga tgatggccag gccctcgtct agagtgccaa
     3421 ccagatcttc cgccggaacg cgaacgccgg acaattcgac ttccgccgcc cggccgccat
     3481 cctgtgtcgg ataggagcgt cgcgagacgc cttcggcgcg ggcgtccacc acgaacacgc
     3541 tgatgccctt gtgatcgcgc cgcccgcccg ccgtccgcgc cgtcacgatc agcctgtccg
     3601 ccgaaccgcc gtgcagcacc agccccttgc gaccgtccag cacataggac gcaccgtcct
     3661 tgcgggcggc gagcgcgaca tcatgcagat cgtagcggga ctgcggctcg gcgaaagcca
     3721 gggtggccgt catgtcgccg gcgatgatcc tcgggatgta ctcggcgcga tgggccgcgt
     3781 tgccgaaacg ccgcagcacg ctgccggcga ggacaacggt cgaaaggtaa ggttccagcg
     3841 cgagcgatcg accgatcgcc tccatgacga tcatgaccga gacgggaccg gcgtcgaacc
     3901 cgccatccgc ttcgctgaag ggcaggccga gcagccccat ctccgcatag gccccccaat
     3961 tctcgcggct ccagccggca tctgaagcct gaattttccg gcgcgtctcg aagtcgttgc
     4021 gggatgcaaa cagccggtca acgctgtcct tgagcagctg ctgttcctcg ttgaggtcga
     4081 aatccatgct gaatgatcct tagaggccga gaatggcctt tgaaatgatg ctgcgctgga
     4141 tctcattcga gccaccgtaa atcgagacct tgcgcagatt gaagtagttc ggcgcgagca
     4201 tggccgccca gtccggtccg atcgccggtt cgttccagcc ctccggatca tgctcgtcgc
     4261 gatacggcaa cgcataggga ccggcgactt caagcagaag ctcgctcacc gattgctgga
     4321 gttccgaacc gcggatcttg agcaccgagg aggcggggtc gggcttgccc cgctcgcgcc
     4381 gcgcctcgcc ggcaacgacg cgcaactgcg tcatttccag cgccttcagt tccacctcga
     4441 ccgccgccag cttctgacgg aaccgcgcac tgtcgatcag cctgccgccc ccctgttcca
     4501 gcgcggcaag ttcgcgcacc cgccgcaacc gtgccttggt gatgccgatg cgggcgatgc
     4561 cagtcctttc gttgcccagc aggaactttg catagtccca tccccggttc tcgacaccga
     4621 caaggttctc caccggcacg cgcacgtcat caaagaacgt ctcgttgacc tcgtgtccgc
     4681 catcgatggt ctggatgggc cgcaccgtga tgcccggcgt cttcatgtcc accagcagaa
     4741 aggagatgcc ctcttgcttc tttgcttccg gactggtgcg ggccagaacg aagatccagt
     4801 ccgcgtattg cgccagcgtc gtccaggtct tctggccgtt gatgacgtag tgatccccgt
     4861 cccgacgcgc cgtcgtcttc agcccggcca gatccgagcc ggcgcctggc tccgaaaagc
     4921 cctggcacca ccagtcatcc atggaggcga tgcgtggcag gaaacgcgtc ttctgctcct
     4981 gcgagccgaa agtgtagatc accggtccga ccatgctgac gccgaacgga agcggctccg
     5041 gtgccggcgt gcgctgcatc tcgtcgcgga agatgtagag cttcaccgca tcccagccgg
     5101 ttccgcccca ttccaccggc cagtgcggca cggaccagcc cctggcgtgc aggatcttct
     5161 gccaacgcac catgtcggcc ttgtccggat ggcgcccctc ggtcagcttc gcgcggatgt
     5221 cctgcgggat cgattcatcg aaaaacgcgc gaacttcggc gcgaaacgct aattcttccg
     5281 gcgtcagtcg caaatccatg gcctgtcctg ttggcgaaat gactgggtcg gacaacgcta
     5341 ccggcatccg gctccgttgt gaacgtcctt ttgactcgca gaccgccgaa ttgagccctg
     5401 agaggctacg cgcccgcccc ctcgactcaa gaccccggat tcaggcaagc ccataacgcc
     5461 agcgccggcg gacgatggcg gcagcctacc agcgcaggac ctgcctgccg gcgaccgcgc
     5521 caacgacggt catggcgact gctgcaatgc tgtaccagaa agcgacgaag agcgtgctgt
     5581 catcggtgca gtgcaatgca tagaggctcg caccaatgcc gaccgcagca agaccgctcg
     5641 ccgccccggc caacgcggcg tttccaggtg cgcgccgacg taaagccagc agcaggccga
     5701 caagaagtgg cagggaaagc gccgggatcg tcgttagaca cacaagcccg ttgcgtccga
     5761 tcatgcgcgc ctgccagcct tcgcttccct gagaggcaag ctctccgccg acgaagaaca
     5821 gagcgagagc caaaggcgcc atccagccga aaaatccccg cccttccggc gccgacagcc
     5881 gttgcaatgc cacagcgccg gcagcggcaa tcgccagcgt cactgcctcc ttccgcacaa
     5941 tcgcgaaaaa cgcctctcca atgtcggcac gcaggcccac accggcccag aaaagcatga
     6001 gcgaaactgc gagcccggca gtcaggcttg cgcccgaaag ccatgactgc cgttcggcat
     6061 tttccgttgc gccggcattg tcagccgcga gcaggcgcac gagatcatcg gtcttcatcg
     6121 aggcaatccc ctgtacatgg ccgacaaccg ggccagggcg cgatggagcg cgacacggac
     6181 cgcgccttcg ctcgatccca gttcactcgc cacttcggcg gcgctacgtc cttcgacgga
     6241 catggcggtg acgatgcgcc ggctgttctg gtcgagctgc gacaggacac gatgaaggtc
     6301 gctcgccgcg ctggaatcgg actcctcggc cggcaggtgg tcagcaaggg catcgacatt
     6361 ctcatgcatg cgaacggact gcgcgcgcag ccgatccgcg atcttgtgcg ccagcacggc
     6421 ctgcgcccag atcgagaagg gccgcgactg atcccaggtg ccgcgcttga gatgaatggc
     6481 catcaatgct tcctgcacga tatcctctat gtctgcattc ccccggccct ggcgcgcgag
     6541 acgcgcccgc acgaaactgc gaaatgcccg tgcgagctcc ccgagaagcg tccggtatgc
     6601 actgtcgtcg cctccgagcg cagcacgcat catccgcgcc cagcgctcct cggtccgaga
     6661 tttctccgat gctgtcgtca tctcattcgc tcgatcccct cgattcgtta cagccaattg
     6721 ccatggatgt ctcgcatggc cagccgatca cgacttcgtg tgaggcgtaa caaaaagcca
     6781 ccgccagccg aactggcttt cgagggccac agcaggcccc gcaacaccga aggagaaacc
     6841 aatatggatc gtcgcaccct gtcgctggcc ctggccgcct ctctgacatc cgcctttgcc
     6901 atggcgccgg ccactgctgt cgcgcaagag aaggagaagt gcttcggcat cgcgctgaag
     6961 ggcaagaacg actgcgccgc cggacccggc acgacatgcg ccggcacctc gaagaccgac
     7021 ttccagggca atgcctggtc ctacgtgccg aagggcacct gcgagaagac cgcttccaag
     7081 acttcgccga ccaagttcgg catgctcaaa gagttcaagt cctgaccagt gaacggttag
     7141 cgacagggag agcgccagtg ctgaaaactc cagaagccag ttcatccggc ttggcacggt
     7201 cggcgctctc ctcgttgccc cggcgcgccg gcatcggctt caagccacag cacttcgagg
     7261 aactctccga acgaggttcc cgaacgggtt ttctggaggt gcacgcagag aactacatgg
     7321 gtgcgggcgg tccggccctg gcccagcttg aacgcctgcg gcgggactat ccgctgtcgc
     7381 tgcatggcgt cgctctgtcc atcggcggcg aggggcctct caacctcgaa catctcacgc
     7441 gcctgcgcat cctgctcgaa cgattccagc cctcggcctt ttccgagcat ttggcctggt
     7501 caagccacgg gccgtactat ttcaatgatc tgctcccggt cgcctatgac gggccgaccc
     7561 ttgatcgcgt ctgcgaacat gtggacttgg cgcagaacac gctcggccgc cgccttctgc
     7621 tcgaaaaccc ttcgacctat ctgcaatttt ccgaaagcag catcgacgag gtcgcgttca
     7681 tctcggaagt cgcgcgccgg accggttgcg gcctgctgct tgacgtcaac aacgtcgcag
     7741 tctcctgcgc caatcacggc cgcgatccgt ttgcctatct tgatgcgttt cccgtcgctt
     7801 gcgttggcga gatacacctt gccggctatg ctgaagagca cgacacattg ggcgcgcccc
     7861 tgcgcatcga cgcgcatggc tctcctgttg aacccgatgt ctgggcgctt ttcaggcata
     7921 tcctgaaccg gacgggaccg atcgcgacgc tgatcgagtg ggacaatgat gtgcccgatc
     7981 ttgcaacgct tctggccgaa gccaaaaagg ccgatgcctg cctcgcaggt tcaggaaacg
     8041 ggtgcagggc agcatgaccg cttcacacca aagcctcctc gcggcggcgt tgcttggccc
     8101 cgacctgacc attcccggac tgaagacctg gaatggttct gatccggttc aacgcttcaa
     8161 cgtctatcgt aacaacgtcg tccattcgct cgttgaagcc ctgcgcgcga aattccctgt
     8221 ggtcgagcac ctggtgggcc ggaccttctt tggtgcgatg gcgcagcact tcgtgcgttc
     8281 ggccccgccg aaatccgtga tcatggcgcg gtatggcgac gaattcccgg attttgtcgc
     8341 atcctatgct cctgccggac cacttgccta tcttgccgac gtggctcgcc tcgaggcagc
     8401 gcgcgtgcgt gcctatcatg ccgctgacgc cagggcgttg ggaccagccg acttcgagac
     8461 catcccgcct catgcccttg ccgaccttgt cttgaccctt caccccaccg tgcgcctgat
     8521 ccgttcagcc catgcgattt acgcactctg ggcagcccat cagggcgagc tggaaattgg
     8581 cgacgtcgat cccgcggttc cgcaagacgt tctggttgtg cggcccgccg gcgaagtgct
     8641 tgtcgcatca ttgccgcagg gcgcggccga gatcctcgaa tgcctggctt cgggcgcaca
     8701 gctcggggca gcgctgatgg cgtccggcac ccccggtctc gaagccattc ccgttttccg
     8761 catgcttgtc atgctcggcg cgttctgcgc cctcagtctg cctggagaaa tcgaatgact
     8821 gccggaatgt tcaacaccgc gatatcgctg ttcgacagga tcgaggacgc gttctcgcgc
     8881 atcccgcaca ccctcatcgc tctcgtcggt cgcattgcca ttgccggaac cttctggaaa
     8941 tccggccaga caaaggtgca gggtttcgcg ctcgatctgt tcgagggacg ctttgagctg
     9001 ggctggccgc gtttcgccga ttcaaccgtt gacctgttcc gggacgagta caagctgccg
     9061 ctgctgccac cggagttcgc cgcgcttgcc gcgaccctgg ccgagcattt ctttccggta
     9121 ttgatcctga tcggacttgc cacacgcctc tccgctgcgg ccttgctggt gatgacgctg
     9181 gtgatccagg tcttcgtcta tccctccgcc tgggcaatcc atggcacctg ggctgccgtg
     9241 ctgctctggc tgatgagcgg cggcgccggg acgatttcgg tcgatcacat gcttcgcaag
     9301 cgcttcggct gaagtcagga cgtgcaagca acgggcggac accccggctc acactcgccg
     9361 tgccgacgat atgtccgctt gcagatctaa gacgacgcca aaaggttctt ggcaccgccg
     9421 gtgccaggct tgcgcacgta gatcccgaag ccaagctccc ggatgaggcc agcgcaccgg
     9481 ctattgagat cctcagcaac ggctttgatc cgtgccagat ccgttccctt gcgccggctc
     9541 atcaggcttc gaaagaccag catgtcgagc gcggtggaat cgagccaatc cacctgttca
     9601 gagtatccgg cgatccacca gggacagaca gcctcctgga acaggtaatc cgccgtctcc
     9661 tgggtcatga aacggcagga ggcaaaatag acgccggaca actgcgagcc ccgcgtggcc
     9721 cggatggcct tcagcatggt tcggaactcg agccgtgaca gcttgtcacc attgtgccag
     9781 gcgattccgt ctggctcgcc atgcgcggcg atatagagga ccgagcagtg gggatctgcc
     9841 gcgatccgcg gaataacttc cttggccgcc cgttttccgc tgaccatttc atagtggtaa
     9901 tggttgggat tggcgaggaa gacatccgaa atcagatcga atatcggcga cacagacgtg
     9961 ttcttcccgg cccaccactt ggattcgacg acggcgagac cggtgtgctt ctgcttctgc
    10021 aattggctgg gcctgcgaac catgcgaact cgcaccaaac ttcctgcaag ttcatgacaa
    10081 tagaacaccc ggttgtcgga gcaagagcat aggccaaatc cgcgaggacc ggaccgcaac
    10141 cgcgacaacc ccgtcgaagc gggtctcgtg caggcttgcc ggaagccgtc atcggcggct
    10201 tccggcatga gctcagttca acgctgcgcc tttcggacgg cgaaccggct gtgaaggcgc
    10261 agatccaacg atcagcccgc ccgggcctgc catgacaggc accgtttcgc catccttcac
    10321 cgaaccggcg aggatcatct cggccaacgg atcctgcagg ttcttctgga tggcgcgctt
    10381 cagcggccgc gcgccatagg cggggtcgta gccggcctcc gccagccagc cacgtgccga
    10441 agcatcaagg tccacagtga tcttgcgttc ctccagcagc ttgcgcagac gctccatctg
    10501 gatgtcgaca attctgccca tatgctcgcg cttgagcctg tggaacagga tgatctcgtc
    10561 gatgcggttg aggaactcgg gacggaaatg tcccttgacc acgcccatca cctcgtcgcg
    10621 cacgccgtcc gtgtcctcgc cttcgccctg gttgacgaga tactcggaac caaggttcga
    10681 ggtcatgatg atcagcgtgt tgcgaaaatc gaccgtgcgc ccctgcccat cggtcaggcg
    10741 gccgtcgtcg agcacctgca agagcacatt gaacacgtcg ggatgggctt tttccacctc
    10801 gtcgaacagc acgacctgat agggccggcg gcgaaccgcc tcggtcaggg cgccgccctc
    10861 ctcatagccg acatagcctg gaggcgcgcc gatcagccgg gccacggaat gcttctccat
    10921 gaactccgac atgtcgatgc gcaccatggc ggtgtcgtcg tcgaacagga agccggcgag
    10981 cgccttggtc agttccgtct tgccgacgcc ggtcggcccc aggaacatga acgagccgat
    11041 cgggcggttc ggatcctgta gcccggcgcg ggcgcgacgc acggcggttg agaccgccga
    11101 gatcgcctcc tcctggccga cgacacgctt gccgatctcg gcctccatgc gcagaagctt
    11161 gtccttctcg ccctccagca tcttgtcgac cggcacgccg gtccagcgcg agacgacctg
    11221 agcgatgtga tcggtggtga ccgcctcctc cagcatgcca ccagagccgg ctgcctcttc
    11281 gatggccttc aactgcttct cgatgccggg aatgacgcca taagcgagtt cgcccgcgcg
    11341 ctggtactcg cccttgcgct gggcgatggc gaggtcgttc cgcgcgccct caagctgctt
    11401 cttgaggtcg gctgccttgc cgagcttgtt cttctcggcc tgccaggacg tggtgagcgc
    11461 ggcggactgc tcctccagca cggccagttc ctgctggagc ttcgccagcc ggtcgcggga
    11521 ggccgcgtcg gtttccttct tcagcgcctc ctgctcgatg cgcaggcgga tgatttcgcg
    11581 gtcgatggaa tcgagcgcct ccggcttcga atccacctgc atgcgcaggc gcgccgaggc
    11641 ctcatcgaca agatcgatgg ccttgtcggg caggaaccgg tcggtgatgt agcgattcga
    11701 cagggttgcg gcggcaacca gcgcggagtc ggtgatgcgc acgccgtgat gctgctcgta
    11761 tttctccttc aggccgcgca ggatcgagac cgtatcctcg acggtcggct cggagacaaa
    11821 cacgggctgg aagcggcggg cgagcgcggc atccttttcc acatgcttcc ggtattcgtc
    11881 gagcgtggtc gcgccgacgc agtgcaattc gccgcgcgcg agcgcgggct tgagcaggtt
    11941 cgaggcgtcc atcgcgccgt cggccttgcc ggccccgacc agcgtatgca tctcgtcgat
    12001 gaacaggatg ataccgcctt ccgccgccat cacttcactc agcacggact tcagccgttc
    12061 ctcgaactcg ccgcggtact tggcgccggc gatcagcgcg cccatgtcga gcgcgagcag
    12121 ggccttgtcc ttcagggatt caggcacgtc gccgttgacg atgcgcagcg ccagcccctc
    12181 gacgattgcc gtcttgccga cgcccggctc gccgatcaga accggattgt tcttggtgcg
    12241 ccgtgacagc acctggatgg cgcgacggat ttcctcgtcg cggccgatca ccgggtcgag
    12301 cttgccgtta cgggcggcct cggtgagatc gcgcgcgtat ttcttcagcg cttcataggc
    12361 gttttcagcg gtggcgctgt cggccgtgcg gcccttgcgg atcgcctcga tggcgccgtt
    12421 gagagcctgg ggcgtgaggc caccctgcgc cagcgccttg ccggcctcgg tgtccttctc
    12481 gaccgccagg gccagcagca tgcgctcgac ggtgacgaag ctgtcgccgg ccttctccgc
    12541 ggccttctcc gccgccacga agacgcgggc gagttgcggc gtcacataga tgttcccggc
    12601 gccagagcct tctactctcg tgtgcctttt caaggcggat tctgtagcct gccgcacggt
    12661 gcgcgggtcg ccgccggcgc gctcgatcag gccgctggca aggccctgct cgtcgtcgag
    12721 aagcgccttc aataggtgct ccggcgcgaa ctgctggtgc ccctcgcgca aggcaatggt
    12781 ctgggccgcc tgcacgaaac cgcgagcgcg ttcggtatac ttgtcgaaat tcatgacaat
    12841 ccctctttca cagcaaggat ggcgccccct tcaggcggcg ctggagtcgt ccggacccgc
    12901 tcctgcggca tccggcctcc cccatgtggg aagccactag cgcaattgga agggggcggc
    12961 catgatccgc agcaaccgag tgccgcgagg atgtcatgac tgcctctgtc gcctttctga
    13021 cgcgctatcc cgtcaaggga ctttcgcccg agccgttgca gtcggccgtc ttgactgccg
    13081 gcgcgcattt tccaggtgac cggatgttcg ccatcgagaa cgggccgtcc ggattcgatc
    13141 cggcggctcc ggtgcatcag ccgaagttca agtatctcat gctcatgagg aatgcggcgc
    13201 tggcgcgctt ccagacccgc tatgacgacg cgagcggcgt gctgaccatc cggcgcggtg
    13261 ccgagacggt cgcgcagggc gacctgcgca ccgaggacgg acgccgcgcg attgagcttt
    13321 tttacgacat ggaactcgcc gacgacatgc gcggcccggc gaaagtgctg atggctcccg
    13381 aagggcatcg ctttatggat tcgcgctcgg gcttcgtctc gctgctcaac cgggcgacgc
    13441 tttcggcgat tgccgccaag gccggccgtc ggggtctcga tccgatgcgg tttcgcgcca
    13501 acatcggcat cgatggcctg ccgccctggg gagaattcga cttggtcggc aaggaagtcc
    13561 gaatcggcga ggcgaccctg cacatcacca agagaattga tcgctgcgcc gccacccatg
    13621 tggaccctgc gacggcggtg cgtgacatcg acatggtcgg cctgctcgaa cgggaattcg
    13681 gccaccacga ctgcggcgtg tatgccgaag tcgtgaccgg tggcacgatc cgtctcggaa
    13741 atgccgtcgc cgcatcctga accagcacgg tcaatccgcg tctttcacca gacgcggtcg
    13801 cctatagtca actcgtcggc tctccctgcg ggtccgaacg ctgttccggc accgcatcaa
    13861 gattcccgcc cgtgctttcg aacgattcgc cgccgccctc gtcgcgcggc ctcaacg
//
LOCUS       DAOMXN010000039        13852 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_2429_length_13852_cov_10.824962, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000039 DAOMXN010000000
VERSION     DAOMXN010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 13852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13852
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2429_length_13852_cov_10.824962"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="PK812_05055"
     CDS             complement(<1..380)
                     /locus_tag="PK812_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRJ68958.1"
                     /translation="MTDKAPDAYRTISEVAEDLDLPQHVLRFWETRFSQIRPLKRGGG
                     RRYYRPDDVELLKGIRYLLYSEGFTIKGVQRILKEQGPKAVQMLVLGPAGAAMAPAPQ
                     PHGAEAYHEDEPGEADDEAPDAADA"
     gene            complement(465..779)
                     /locus_tag="PK812_05060"
     CDS             complement(465..779)
                     /locus_tag="PK812_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HRJ68959.1"
                     /translation="MTGRTVTRADLCDAVYQRLGLSRAESADLVELVLGEISDCLERG
                     ESVKLSSFGVFAVRDKGERIGRNPKTGVEVPITSRRVLTFKPSNVLRARINGLEAADE
                     DE"
     gene            complement(938..1915)
                     /locus_tag="PK812_05065"
     CDS             complement(938..1915)
                     /locus_tag="PK812_05065"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HRJ68960.1"
                     /translation="MVKRRAVVRGVGSYLPARVVTNAELAERIETSDEWIVQRSGIRS
                     RHVAADGETTSMLATRAAEAALTDAGVKAGEIDLIVLATATPDYTFPATATQVQHNLG
                     ITRGVAFDVAAVCSGFVFALATAEKFIASGSHERALVIGAETFSRILDWNDRTTCVLF
                     GDGAGAVVVEAQAGQGTTSDRGVLTTHLRSDGRHRAKLFVDGGPSTTGTAGHLRMEGR
                     EVFKHAVGMITDVITDAFAATGTTANDLDWFVPHQANRRIITASADKLDIAQEKVVIT
                     VDDHGNTSAASIPLALDVAVKDGRIRQGHLVMLEAMGGGFTWGSALVRW"
     gene            complement(1909..2967)
                     /gene="plsX"
                     /locus_tag="PK812_05070"
     CDS             complement(1909..2967)
                     /gene="plsX"
                     /locus_tag="PK812_05070"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HRJ68961.1"
                     /translation="MTRPVRIALDAMGGDYGPSVVVPGADLALERHPDSSFLLFGRES
                     EIAPILAKHPRLQARSELRHTDVVVAMDAKPSQALRSGRYKSSMWLAVEAVKKGEADV
                     AVSAGNTGALMAMSRLCLRMMPGIDRPAIAAMWPNVRGESIVLDVGASIGADAGNLVD
                     MAIMGAAMASIVFDLERPTVGLLNVGVEEIKGVEQVREAGRILREANLPSIEYHGFIE
                     GDDIGKGTTDVVVTEGFSGNIALKTAEGTAKQIGEYLRSAMSRTIMSKIGYLFARGAF
                     RALKDKMDPRKVNGGVFLGLDGVVIKSHGGTDAIGFASAVDIAYEMVRYELLSKIRET
                     ITSSRIAPEPAATQEPQS"
     gene            complement(3116..3661)
                     /locus_tag="PK812_05075"
     CDS             complement(3116..3661)
                     /locus_tag="PK812_05075"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HRJ68962.1"
                     /translation="MNPGKKASQGWIDPLLRPVAAHAIPSAGLDVTVAPDDAERAALA
                     RALNLAAVQSLSARYRLTHAAGGAIHVLGEIVARVKPVCVVTLEAFDLEIREPVDVRF
                     AEEQDERDADEFDMLAGDEPPDPMENGMLDLGKVTTEFLSLAVPPFPRKPDAAFSLKE
                     EEPAARPFAGLAALKPGKSAR"
     gene            complement(3689..4198)
                     /locus_tag="PK812_05080"
     CDS             complement(3689..4198)
                     /locus_tag="PK812_05080"
                     /inference="COORDINATES: protein motif:HMM:NF015914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome C chaperone family protein"
                     /protein_id="HRJ68963.1"
                     /translation="MFTSLFRRRAGPDIDPLYGAIMAAALRPHLYASFGVPDTFEGRF
                     ECATLHMALVLVRLRQLPPPADAAGQALVDRFFDGLDAAIREKGIGDVAVPRRMKKFM
                     QGFYGRVAAYEQAGGDPAALAVAIGRNVLEGANASRDLVDDVIASQEAVMHASLEDLL
                     ERPAQILPR"
     gene            4351..4809
                     /gene="bamE"
                     /locus_tag="PK812_05085"
     CDS             4351..4809
                     /gene="bamE"
                     /locus_tag="PK812_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HRJ68964.1"
                     /translation="MKLFPLGLALSGALLVAACGQEPQTRGYVVDDRALDQIKPGVSA
                     ESVILILGTPSMVSTVGGKGYYYVSQKVAQRYQFMEAKVTDQRVIAVHIDAKNKVERV
                     ANYGIEDGKIFDFISRTTPSGGEEMTLVRQILRANNLFNPNAPAGSQPGS"
     gene            complement(4841..6004)
                     /locus_tag="PK812_05090"
     CDS             complement(4841..6004)
                     /locus_tag="PK812_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRJ68965.1"
                     /translation="MKPLDLTAHFSRFLAVPGRIHLAAHSHHFWPDVTREAQLLAWDD
                     ACRHADEKWEPLFAEVIPAVQRAIAAVLGLPAPQSIAFAPNTHDFVRRLLSTLPERPR
                     ILTTDGEFHSFARQVARLEEEGLIQVERVPHEPVTSFAERLRAAAARGGHHMVFVSHV
                     IFNAGCTSGDIAALAAAVPDRGTLIVIDGYHGFMALPTDLSAVAGRVFYIAGGYKYAM
                     GGENVCFMHCPPGYGERPRDTGWFAAFGALAGRQQGVPYGPDGNRFWGATFDPIGFYR
                     LRAVFSWLEGLGLRVQDIHDHVLALQEHFLACVAARRIGALQVARLVTPMETRQRGHF
                     LTFETPEAGALYERLLARRIVTDVRGDRIRFGFGLYHTALSVEQAADAISAAL"
     gene            complement(6162..8315)
                     /locus_tag="PK812_05095"
     CDS             complement(6162..8315)
                     /locus_tag="PK812_05095"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019857622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HRJ68966.1"
                     /translation="MTALWIVILLGALSIVYAVVTINQVMASDAGNQRMQEISGAVAE
                     GAQAYLNRQYTTIAVVGAVIFVGAWLLLGLAVAIGFLIGAVLSGAAGYIGMNVSVRAN
                     VRTAAAAIKSLAGGLDIAFKAGAVTGMLVAGLALLGVAIYYAILTAGMGLSPSSRTVI
                     DSLVALGFGASLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPATIADNVG
                     DNVGDCAGMAADLFETYAVTVVATMVLAAIFFAATPNLAAAMVYPLMICGVCIVTSII
                     GTYFVKLGDNGSIMGALYKGLIATGVLSVAGLAAANQFTIGWGEIGKVSGQTITGAGL
                     FWCGVIGLVVTALIVVITEYYTGTGKRPVVSIAQASVTGHGTNVIQGLAVSLESTALP
                     ALVIVAGIIGTYQLAGLFGTAIAATTMLGLAGMIVALDAFGPVTDNAGGIAEMAGLPK
                     EVRHSTDALDAVGNTTKAVTKGYAIGSAGLGALVLFAAYSYDLNYFIAEANKAGSTLY
                     PYFKGMTQVTFDLSSPYVVAGLIFGGLIPYLFGGIAMTAVGKAAGSVVVEVRRQFREM
                     PGIMQGTQKPDYARAVDLLTRAAIKEMIIPSLLPVLAPVVAYFLVLWVSGSKASAFAA
                     LGASLLGVIVNGLFVAISMTSGGGAWDNAKKSFEDGFVDQDGVKHLKGSDAHKASVTG
                     DTVGDPYKDTAGPAVNPAIKITNIVALLLLAILAH"
     gene            complement(8541..9749)
                     /locus_tag="PK812_05100"
     CDS             complement(8541..9749)
                     /locus_tag="PK812_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019962532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRJ68967.1"
                     /translation="MTTELSAGDRLARRNAFILAAGSALGGANASVVIATGSLIGQAM
                     APSPALATVPVTTFVLGTALFAMPASNLMRAVGRRQGFTLGSLLGILAGLVGATAIWL
                     GSFWLYSIATALAGAYQSFIVLYRYAAADSATPAFRPKAVSWVMAGGVAAAFVGPQLT
                     IWTKDAFPPLLFLGTYLGQAAFAIIAMGVVSRFQDTSLTAAELAGSDRPLREILRDHR
                     VKVAILCGMTGQAMMNMVMTATPIAMIGCGFTVTESTLGIQWHVISMFAPAFFTGSVI
                     GRFGKGPVIAIGLVLLALAGAINLAGITLANFWGSLIVLGIGWNFAFTGATALLLDYH
                     TPKERGRVQGFADFLIFGATTLASFLAGYLYNIYGWSAVNWMVVPVTLFTFAMVFAVG
                     LLKSPPKPAV"
     gene            9828..10490
                     /locus_tag="PK812_05105"
     CDS             9828..10490
                     /locus_tag="PK812_05105"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRJ68968.1"
                     /translation="MSATLLFDLDGTLCETDWAHLKAFEIVFGELGIAMNEALFKARI
                     MGASNPMIGAAFLPHLPAGERDRVLDHKEEVYRGLIGTVAPVAGLVDLLDWADAQGLA
                     CAIVTNAPRVNADLIIDGLGLRARFRAIVCGQELARGKPDPLPYLEGLRLLGGEARRS
                     VAFEDSPSGMQAALGAGLALVGMTTNLDGPTVLGHGAGVAVRDFTDPAVREFVMERLR
                     AA"
     gene            10543..11055
                     /locus_tag="PK812_05110"
     CDS             10543..11055
                     /locus_tag="PK812_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HRJ68969.1"
                     /translation="MRLVAMFAVAGILCGCVAKDVQIETAFDPAAAAFIKAEGSATIE
                     GHAFLKRWDGRAIHAVGEHAWLVPVTPYSEERFRKLYGDRKLMPAVLQNRVGNPDPQF
                     MEYVRRTKTESDGTFRFEKVAPGAYFLVTTVTYREKHEDMLPQGGSVYERVTVKGDEK
                     AIIRVVLTDK"
     gene            complement(11113..12219)
                     /locus_tag="PK812_05115"
     CDS             complement(11113..12219)
                     /locus_tag="PK812_05115"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HRJ68970.1"
                     /translation="MATAPYGSVKKTGASGDDTISLSGSFPLSSLNATGGAGKDFITG
                     TSWDDLISGGSDNDTVLGEGGDDVVSGDAGADMVFGGSGNDVLFGDGWSESASDGNDL
                     ILGEAGYDTIFGGGGADYILGGDDADSISGGAGDDTIGGDNYVGASGTSSGSGHDTIS
                     GGLGQDKIYGGGGNDLIYGDAGNDTILGEDGADSIYGGTGNDHILGQGSNDYLNGGDG
                     DDTIGGGEGKDGLDGQMGDDQLEGGAGNDTLNGGSGNDKLDGGADNDYLIGASGNDLV
                     MGGAGNDTLWGDEGTDTLYGGAGADSFRSSGASDGTDTIKDYNKAEGDVIDILGPTLG
                     FQVVQVGANAVVVDASNNALFIVENTNKDDVTIV"
     gene            complement(12319..13320)
                     /gene="thiL"
                     /locus_tag="PK812_05120"
     CDS             complement(12319..13320)
                     /gene="thiL"
                     /locus_tag="PK812_05120"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HRJ68971.1"
                     /translation="MSPGERPGEFDLIARYFAPMAGAGGLGLVDDAALLTPPPGCDLV
                     LTKDALVAGVHFFPNDPPETIARKALRVNLSDLAAKGARPLGFLLGLGLPTDWREDWL
                     ATFASGLAADSALFGCPLLGGDTVRAGGPLILSITALGSMPAGTMVRRSGGKPGDLLY
                     VSGVIGAAALGLHLIRDGDPPWSRPLSGDARAALISAFHVPQPPVALAEGIRLHASAA
                     MDVSDGLIGDALKLAKASGTGLEIEAARVPLPLPGTPLPTLVARHRFLLDAVLTGGDD
                     YQVLAAVPPDRASAFERAAQDIALPVTPIGRLTARGVRIHGFAGEDLSFGKPSFGHF"
     gene            complement(13348..13809)
                     /gene="nusB"
                     /locus_tag="PK812_05125"
     CDS             complement(13348..13809)
                     /gene="nusB"
                     /locus_tag="PK812_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRJ68972.1"
                     /translation="MTRGEKRSAARLSAVQALYQMDLTGKGVIEVFSEFERFWIGQEI
                     EGDQYKPAERSLFRQIVQGVIEHQVAIDRAVDRILSDGWPLKRVEALMRASLRGGAYE
                     LMKTPDVPVKVVVSEYVDIASAFLDKDEVGMVNAVLDRLARELRAEELARG"
BASE COUNT         2440 a         4593 c         4586 g         2233 t
ORIGIN      
        1 gcatcggcgg catccggagc ctcgtcgtca gcctcgcccg gttcgtcttc gtggtaggct
       61 tcggcgccgt gcggctgggg cgcgggcgcc attgccgcgc ccgccggccc gagcaccagc
      121 atctgcaccg cctttgggcc ctgctccttg agaatgcgct gcacgccctt gatcgtgaag
      181 ccttcggaat agagcaggta gcggatgccc ttcagcaact ccacgtcgtc cgggcgatag
      241 tagcggcggc ccccgccgcg cttgagcggg cggatctgcg agaaccgtgt ttcccagaag
      301 cgcagcacat gctgtggcag gtcgaggtcc tcggcgacct cgctgatggt gcggtaggcg
      361 tccggggcct tgtcggtcaa acgtcatctc cctgcgggag atgcgaatca ccccgccggt
      421 ccatgctaga cctgtttaaa tcgccgttga atcggcaagg ccggctattc gtcctcgtcc
      481 gctgcttcca acccgttgat ccgtgcccgc agcacgttcg agggtttgaa cgtcagcacg
      541 cggcgcgagg tgatcggcac ctcgaccccg gtcttcggat tgcggccgat acgctcgccc
      601 ttgtcgcgca ccgcaaagac gccgaacgac gaaagcttca ccgactcgcc gcgttcgaga
      661 caatccgaga tttcgcccaa aaccagctcc accagatcgg cggattcggc gcgcgacagc
      721 ccaagcctct gatagaccgc atcacagagg tcggcgcggg taactgtgcg tcccgtcatg
      781 ttcttgctct cccaacccga ggcaatccgt atgctactga aaactcgacc ttattgctgg
      841 aaaccggacc ggtcaacccg ggcgccgcgg tattggcggc atcgctttcc catacgcccc
      901 gtgcggcgca gcgccgcagt cggcgcctgc cgacggttca ccagcgcacc agcgccgatc
      961 cccacgtgaa gccaccgccc atcgcttcga gcatcacgag atggccctgg cggatgcgtc
     1021 cgtccttcac cgcgacgtcg agcgccagcg gaatcgaggc agcggaggta ttgccgtggt
     1081 catcgaccgt gatcacaacc ttctcctgcg cgatatccag cttgtcggcc gaagcggtga
     1141 tgatccggcg gttagcctgg tgcggcacga accagtcgag atcgttggcg gtagtgccgg
     1201 tggcggcgaa agcatcggtg atcacatcgg tgatcatgcc gacagcatgc ttgaacacct
     1261 cgcggccttc catgcgcaga tggcccgccg tgcccgtggt cgaggggcca ccgtcaacga
     1321 agagcttggc gcggtgccgg ccgtcggaac gcagatgcgt ggtcagcacg ccgcgatccg
     1381 acgtcgtgcc ctgcccggct tgcgcctcca cgacgaccgc tccggcaccg tcgccaaaca
     1441 gcacacaggt cgtgcgatcg ttccagtcga ggatgcgcga gaaggtttcc gcgccgatca
     1501 ccagcgcccg ctcgtgcgaa ccggaggcaa tgaacttctc ggcggtcgcg agggcaaaca
     1561 cgaaaccgga gcacacggca gccacatcga aggcaacgcc gcgggttatg ccgaggttgt
     1621 gctgaacctg cgttgccgtc gcgggaaagg tatagtctgg cgtcgccgtg gcgagcacga
     1681 tgaggtcgat ctcgccggcc ttgaccccgg catcggtgag cgccgcctcg gcggcccggg
     1741 tcgcgagcat gctggtggtc tcgccgtcgg cggccacgtg ccggcttcga atgcccgaac
     1801 gctgcacgat ccactcgtcc gaggtctcga tgcgttcggc gagttcggca ttggtgacga
     1861 cgcgggctgg cagatagctg cccaccccgc gcacgacggc gcgacgtttc acgattgcgg
     1921 ctcctgcgtt gcggccggct cgggagcgat gcgcgacgaa gtgatggttt cgcggatctt
     1981 cgagagcagt tcgtaacgaa ccatctcgta ggcgatatcg acggcggagg cgaatccgat
     2041 ggcgtccgtg ccgccgtggc tcttgatgac gacgccatca aggccgagga acacgccgcc
     2101 attgaccttg cgcgggtcca tcttgtcctt aagcgcccgg aatgcgcccc gcgcgaacag
     2161 ataaccgatc ttcgacatga tggtccggct catggcggac cgcaggtact cgccgatctg
     2221 cttggccgtg ccctcggcgg tcttcagcgc gatattgccc gagaacccct cggtcaccac
     2281 cacatcggtc gtgcccttgc cgatgtcgtc gccctcgatg aaaccgtggt actcgatgct
     2341 cggcaggttg gcctcgcgca ggatgcggcc ggcttccctc acctgttcga cgcccttgat
     2401 ctcctcgacc ccgacgttca gcaggccgac ggtggggcgt tcgaggtcga agacgatgct
     2461 ggccatcgcc gcgcccatga tcgccatgtc gacgagattg ccggcatccg cgccgatgga
     2521 ggcgccgaca tcgagcacga tcgactcacc gcgcacattc ggccacatcg ccgcgatcgc
     2581 cggccggtcg atgcccggca tcatgcgcag gcacagacgc gacatggcca tcagcgcgcc
     2641 cgtgttgccg gcggaaaccg cgacatccgc ctcgcccttc ttcaccgcct ccaccgccag
     2701 ccacatcgac gacttgtagc ggcccgagcg cagcgcctgg ctcggcttgg cgtccatcgc
     2761 caccacaaca tcggtatggc gcaactcgga cctggcctga aggcgcgggt gcttggcgag
     2821 gatgggcgcg atctcgcttt cccggccaaa cagcagaaaa ctcgaatcgg gatggcgctc
     2881 cagcgccaga tcggccccgg gaacaaccac ggacggaccg taatcgcccc ccatggcgtc
     2941 caacgcaatg cgcaccggtc tggtcatggc gaagcgaaaa atccgtttct gcgcgccaat
     3001 ctcgcccggg caggcaggtg cgcgcccggc aaataagcac aagcgcacgg gctggcaagg
     3061 cttgccttgg tccaatctcc gaccaaagcc gatgcctcgc ctggcgaccg gcccatcacc
     3121 gggcagactt tccgggcttg agggctgcca atccggcgaa cggcctggcc gcgggctctt
     3181 cctccttcaa tgagaaggct gcgtcgggct tgcgcggaaa gggcgggacc gccagcgaca
     3241 ggaactccgt cgtcaccttg cccaggtcga gcatgccgtt ctccatcgga tccggcggct
     3301 cgtcgccggc gagcatgtcg aactcgtccg catcgcgctc gtcctgttcc tcggcgaaac
     3361 ggacgtccac aggttcgcgg atctcaagat cgaacgcctc cagcgtgacc acgcacacgg
     3421 gcttgacccg ggcgacgatc tcgccgagga catggatcgc tcccccggcc gcatgcgtca
     3481 ggcgatagcg cgcggagagg gactgcacgg cggcaaggtt caaggcccgg gccagcgcgg
     3541 cgcgctctgc gtcatccggc gcgacggtca cgtcgaggcc ggcggaggga atggcatggg
     3601 cagcgaccgg ccgcagcagg ggatcgatcc agccttggct tgctttcttt cccgggttca
     3661 ttggcaagtc ccgatctggt tgaactgact aacgcggcag gatctgcgcc gggcgttcga
     3721 gaaggtcttc aagggaagcg tgcatcaccg cttcctgcga tgcaatcaca tcgtcgacaa
     3781 ggtctcggct ggcgttcgct ccttccagaa cgttccgtcc aatagccacg gcaagcgccg
     3841 cgggatcgcc gccggcctgc tcataggccg cgacgcgacc atagaaccct tgcatgaatt
     3901 tcttcatgcg ccggggcacc gcgacgtctc caatcccctt ctcgcgaatc gccgcatcca
     3961 gcccatcgaa gaagcggtcg acgagcgcct gcccggcggc atctgccggc gggggaagct
     4021 gcctgagacg cacgagcacc agcgccatgt ggagtgttgc gcactcgaac cgcccctcaa
     4081 acgtatcggg cacgccgaaa ctggcgtaga ggtgtggtcg cagggccgcc gccatgatgg
     4141 cgccgtaaag cgggtcgatg tcgggaccgg cgcgccgccg gaacagggaa gtgaacattc
     4201 tcaacggttt ccggccgggc ttggttgcaa attgcaccat cccgggctag ccaaacggat
     4261 gttgacgcgt gctccttagc gcaggcgggc caggcagggc gagcattttt cggatgcgct
     4321 gcgggcgacg agggtatcgg agttagggca atgaagctgt ttcctctcgg tctggcgctt
     4381 tccggcgcgc ttctggtggc cgcctgcggc caggaaccgc agacgcgcgg atatgtggtg
     4441 gacgacaggg cccttgatca gatcaagccg ggtgtcagcg ccgagagcgt gatcctgatc
     4501 ctcggcacgc cctcgatggt gtcgaccgtc ggcggcaagg gctattacta cgtcagccag
     4561 aaggtcgcgc agcgctacca gttcatggaa gccaaggtca ccgaccagcg cgtcatcgcc
     4621 gtccatatcg acgccaagaa caaggtcgag cgcgttgcca attatggcat agaggatggc
     4681 aagatcttcg atttcatcag ccgcaccacg ccttccggcg gcgaggaaat gacgctggtc
     4741 cgccagatcc tgcgcgccaa caacctgttc aacccgaacg cgccggccgg ctcgcagccg
     4801 gggagctgag gttggcgggt cggcgcccgc ggcgcgctcg ctacagcgct gcggaaatgg
     4861 cgtcggcagc ctgctcgacc gacaaggccg tatgatagag gccaaatccg aaccggatgc
     4921 ggtcgccgcg cacgtccgtg acgattcggc gcgcgagcag gcgctcgtac agcgcaccgg
     4981 cctcaggtgt ctcgaaggtc aggaagtgcc cgcgctggcg cgtctccatc ggcgtcacga
     5041 gtctggccac ctgcagcgcg ccgatgcgcc gggccgccac gcaggccagg aagtgctcct
     5101 gcagggccag cacgtggtcg tggatgtctt gcacgcgcag gccgaggcct tccagccagg
     5161 agaaaacggc gcgcagccgg tagaagccga tcggatcgaa ggtcgcgccc cagaagcggt
     5221 tgccgtcggg cccatacggc acgccctgct gccggccggc gagggcgccg aaggcagcga
     5281 accagccggt gtcgcgcggg cgctcgccat agccgggcgg gcaatgcatg aagcagacgt
     5341 tctcaccgcc catggcgtat ttgtaaccgc ccgcgatgta gaatacacga ccggccaccg
     5401 ccgacaggtc ggtcggcagg gccatgaagc catggtagcc gtcgatcacg atcagcgtcc
     5461 cgcgatccgg cacggcggcg gccagcgcgg cgatgtcgcc ggacgtgcag cctgcattga
     5521 agatgacatg gctgacgaag accatgtggt gcccgccgcg cgccgccgcc gcgcgcagcc
     5581 gctccgcgaa actggtgacc ggctcgtgcg ggacgcgctc gacctggatc aggccttcct
     5641 cctccagccg ggcgacctgg cgggcgaagg aatggaactc cccatccgtc gtcagaatgc
     5701 gcggacgctc cggcagggtg gacagcagcc ggcggacgaa atcgtgggtg ttcggggcaa
     5761 aggcgatgct ctgcggcgcg ggcagaccca gcaccgccgc gatcgcgcgc tgcacggcgg
     5821 ggatgacctc ggcgaagagc ggttcccatt tctcgtcggc gtggcgacag gcgtcgtccc
     5881 aggccagcag ctgggcctcg cgcgtcacgt cgggccagaa atgatggctg tgcgccgcca
     5941 gatgaatgcg gcccggcacg gccaggaaac ggctgaaatg cgctgtcagg tcgaggggct
     6001 tcatcccggc tgtccttgat ccccgctggg cgggtctggg cacgggccaa tcagcaggcc
     6061 cgcatggtct catcctatcc cagcgcggca tgacgcacag agcaaggcaa caaaaaaggg
     6121 tggggtttcc cccgcccttt ttgagtgcgc ctgacccgcg ctcagtgcgc gagaatcgcc
     6181 agcagcagca gcgccacgat gttggtgatc ttgatggccg ggttcacggc cgggccggcg
     6241 gtgtccttgt aggggtcgcc gacggtgtcg ccggtgaccg acgccttgtg cgcgtccgag
     6301 cccttcaggt gcttcacgcc gtcctggtcg acaaagccgt cctcgaagga cttcttggcg
     6361 ttgtcccagg cgccgccgcc ggaggtcatc gagatcgcca cgaacaggcc gttgacgatc
     6421 acgccgagca gcgaggcgcc gagagccgcg aaggccgagg ccttcgagcc ggaaacccac
     6481 agcaccagga agtaggcgac aaccggcgca agcaccggca gcagagacgg gatgatcatc
     6541 tccttgatcg cggccctggt gagcaggtcg acggcgcggg cgtagtcagg cttctgcgtt
     6601 ccctgcatga tgccgggcat ttcgcggaac tgacggcgca cctcgacgac caccgaaccc
     6661 gccgccttgc cgacggcggt catcgcgatg ccgccgaaga ggtaggggat caggccgccg
     6721 aagatcaggc ccgccaccac gtaggggctg gacaggtcga aggtgacctg ggtcatgccc
     6781 ttgaaatagg ggtagagggt cgaaccggcc ttgttggcct cggcgatgaa gtagttcaga
     6841 tcgtagctgt aggccgcgaa cagaaccagc gcgccaaggc cagccgaacc gatggcatag
     6901 cccttggtca cggccttggt ggtgttgccg accgcgtcga gcgcgtcggt ggagtggcgc
     6961 acttccttcg gcaggccggc catttcggcg atgccgccgg cgttgtccgt caccggaccg
     7021 aaggcgtcca gcgcgacgat catgccggcg aggccgagca tggtggtcgc cgcgatggcg
     7081 gtgccgaaca ggccggcgag ctggtaggtg ccgatgatgc ccgcgacgat gaccagcgcc
     7141 ggcagcgccg tcgattccag cgagaccgcg aggccctgga tgacgttggt gccatggccg
     7201 gtgaccgagg cctgggcgat cgacacgacc ggacgcttgc ccgtgccggt gtagtactcg
     7261 gtgatcacca cgatcagagc cgtgacgacg aggccgatga cgccgcacca gaacaggccc
     7321 gcgccggtga tggtctggcc gctgaccttg ccgatctcgc cccagccaat ggtgaactgg
     7381 ttcgccgcag caaggccggc gacggacagc acgccggtgg cgatgaggcc cttgtaaagc
     7441 gcgcccatga tcgagccgtt gtcgccgagc ttcacgaaat aggtaccgat gatcgaggtg
     7501 acgatgcaga cgccgcagat catcagcgga tagaccatcg cggccgccag gttcggcgtg
     7561 gcggcgaaga agatcgcggc cagaaccatg gtggcgacca cggtcaccgc ataggtctcg
     7621 aacaggtcgg cggccatgcc ggcgcagtcg ccgacgttgt cgcccacgtt gtcggcgatc
     7681 gtcgcggggt tgcgcggatc atcctccggg atgcccgctt ccaccttgcc gacgaggtcg
     7741 gcgccgacgt ccgcgccctt ggtgaagatg ccaccgccca gacgcgcgaa gatcgagatc
     7801 agcgaggcgc cgaagccgag agcgacgagc gagtcaatca cggtgcggct ggatgggctc
     7861 aggcccatgc cggcggtcag gatcgcgtaa taaatggcca cgccgagcag cgcgagaccc
     7921 gccaccagca tgccggtgac cgcgcccgcc ttgaaggcga tgtccaggcc gcccgccagc
     7981 gacttgatcg ccgccgccgc cgtgcgcacg ttggcgcgga ccgagacgtt catgccgatg
     8041 tagcccgccg cgcccgagag caccgcgccg atcaggaaac cgatggcaac cgccaggccc
     8101 agcagcagcc aggcgccgac aaagatcacc gcgccaacaa cggcgatggt cgtgtactgg
     8161 cggttcagat aagcctgcgc gccttctgcg acggcgcccg aaatctcctg catgcgctgg
     8221 ttgccggcgt ccgaggccat cacctgattg atggtgacga ccgcgtaaac gatcgacaac
     8281 gcgccgagaa ggatgacaat ccatagagcg gtcatacttt gcccctgtgc gttgaaagtc
     8341 ccgccgctag catccccagc cgggcggagt ccggatgccc cttagcaaaa ggtaaacgca
     8401 agcgccaacc gtaattcgac ttccggtgca ggacttttgc aggggggtgg tatggcgcgc
     8461 cggggcactg cctcgcatcc cggcgctccg gcgaggcgcc cgggtgccgt ggaaacgtcc
     8521 gcgatccggc gatgatgccc ctagacggcc ggttttgggg ggctcttcaa cagtccaacg
     8581 gcgaaaacca tggcgaaggt gaagagggtc accggcacga ccatccagtt caccgcgctc
     8641 cagccgtaga tgttgtagag gtagccggcg aggaaggagg caagcgtcgt cgcgccgaaa
     8701 atcagaaagt cggcgaagcc ctgcacgcgc ccgcgctcct tcggcgtgtg gtaatccagc
     8761 aacaaggcgg tggcgccggt gaaggcgaag ttccagccga tgcccagcac gatcaggctg
     8821 ccccagaaat tcgcgagggt gatacctgcc aggttgatcg cgccggccag cgcgagaagc
     8881 accagaccga tggcgatgac cggccccttg ccgaaccggc cgatcacaga tccggtgaaa
     8941 aacgccgggg caaacatgga aatgacgtgc cactgaatgc ccagtgtcga ttccgtcacc
     9001 gtgaagccgc agccgatcat ggcgatggga gtcgccgtca tgaccatgtt catcatggcc
     9061 tgtccggtca tgccgcacag gatcgccacc ttgacgcggt ggtcgcgcag gatctcgcgc
     9121 aagggtcgat cgcttcccgc aagctcggcc gccgtcagcg atgtatcctg aaaccgtgag
     9181 acgacgccca tggcgatgat ggcgaacgcc gcctgaccca ggtaggtgcc caggaacagc
     9241 agcggcggaa acgcgtcctt ggtccagatc gtcagctgcg gcccgacaaa ggccgccgcc
     9301 acgccgccgg ccatgaccca ggataccgcc ttcggccgga aggccggcgt ggcgctgtcg
     9361 gcggccgcat agcgatagag cacgatgaac gactggtagg cgccggccag cgccgtggcg
     9421 atgctgtaaa gccagaatga gccgagccag atcgccgtcg cgcctacgag gcccgcgaga
     9481 atgccgagca ggctgccgag ggtgaagccc tgccgccggc cgacggcgcg catgaggttc
     9541 gacgccggca tggcgaacaa cgccgttccc agcacgaagg tcgtgaccgg cacggtggca
     9601 agcgcgggtg agggggccat ggcctggccg atcagcgagc ccgtagcgat gacgacggag
     9661 gcgttcgcac cgcccagcgc cgagccggca gccagaatga aggcattgcg ccgcgccagg
     9721 cggtcgcctg cggaaagttc ggttgtcacg ccatgctgct ccttgatgcg cttgactagc
     9781 cgatgcgttg cgggcacgcc agccgcctat cggggaggaa gtcgcttttg tccgctacgc
     9841 ttctgtttga tctcgacggc accctctgtg aaaccgactg ggcccatctt aaggccttcg
     9901 agatcgtgtt cggcgagctc ggcatcgcca tgaacgaggc gctgttcaag gcgcgcatca
     9961 tgggggcatc caacccgatg atcggcgcgg cgttcctgcc ccatcttcct gcaggcgagc
    10021 gcgaccgcgt tctcgaccac aaggaagagg tctatcgcgg cctgatcggc acggtggcgc
    10081 cggtcgctgg tctggtcgat ctcctggact gggcggacgc gcaaggccta gcctgcgcca
    10141 tcgtcaccaa tgcgccgcgc gtcaacgccg acctgatcat cgatgggctg gggttgagag
    10201 cacggttccg cgccatcgtc tgcgggcagg aactggcgcg gggcaagccc gatcccctgc
    10261 cctatctcga aggtctgcgg ctgttgggtg gcgaggcccg gcgttccgtc gctttcgagg
    10321 attccccctc cggcatgcaa gcggcactcg gcgccggtct ggcgctggtc ggcatgacca
    10381 cgaaccttga tggccccacc gtgctcggcc atggcgcggg cgtcgccgtg cgagacttca
    10441 ccgatccggc cgtgcgtgaa ttcgtcatgg agcgtttgcg cgcggcttga ttccggcctg
    10501 gcccggcgcg agggtggccg gggcagcagc gggtggcgtc tcatgcgctt ggtggcaatg
    10561 ttcgcggtgg caggcatcct atgtggatgc gtcgcgaagg acgtgcagat cgaaaccgcg
    10621 ttcgatccgg ccgccgctgc cttcatcaag gcggaggggt ccgcgaccat cgagggccat
    10681 gccttcctca agcgctggga cggtcgcgcc atccatgccg tgggcgaaca tgcctggctg
    10741 gtgccggtga cgccctattc ggaagagcgc ttccggaaac tttacggcga caggaagctg
    10801 atgccagcgg tgttgcagaa ccgtgtcgga aaccccgatc cgcagttcat ggagtatgtg
    10861 cggcgcacca agaccgagtc agatggcacc ttccgcttcg agaaggtggc gccgggcgcg
    10921 tatttcctcg tcaccaccgt gacctatcgc gagaaacacg aggacatgct gccgcagggc
    10981 ggctccgtct atgagcgcgt caccgtgaag ggcgatgaga aagcgatcat ccgcgtggtg
    11041 ctgacggaca aatgaaggtg ccgccggcag gaaccggcgg ctccggggtg caggccgggc
    11101 agactccggc gttcagacga tcgtgacatc gtccttgttg gtgttctcga cgatgaacag
    11161 ggcgttgttt gaggcatcca ccacgaccgc attggcgccg acctgcacga cctggaagcc
    11221 aagtgtcggg ccgaggatgt cgatgacatc gccttccgcc ttgttgtagt ccttgatcgt
    11281 gtcggtgccg tccgaagcgc cggaagaccg gaaggagtcg gctccggcac cgccataaag
    11341 cgtgtccgtg ccctcgtcgc cccatagcgt gtcgttgccg gcgccgccca tgacaaggtc
    11401 gtttccggag gcgccaatca ggtagtcgtt gtcagcaccg ccatcgagct tgtcgttgcc
    11461 tgaaccgcca ttcagggtat cgtttcccgc gccgccctca agttggtcgt cgcccatctg
    11521 accgtcgagg ccgtccttgc cttcgccgcc gccgatggtg tcgtcgccat cgccgccgtt
    11581 caggtaatcg ttgctgccct ggccaaggat atgatcattg cccgtgccgc catagatcga
    11641 atccgcgccg tcttcgccga gaatcgtatc gttgccggca tcgccgtaga tcagatcatt
    11701 gcccccgccg ccgtagatct tgtcctggcc aaggccgccg gaaatcgtgt cgtggcctga
    11761 gccgctggag gtgcccgagg cgccgacgta attgtcgccg ccgatcgtgt cgtcgccggc
    11821 accgccgctg atggagtcag cgtcatcgcc tccgagaatg tagtccgcgc cgccgccgcc
    11881 gaagatcgtg tcatagccgg cttcgccgag aatcagatcg ttgccgtcgc tggcggactc
    11941 tgaccagcca tcaccaaaca atacgtcatt gccggagccg ccgaacacca tgtccgcgcc
    12001 ggcatcgccg ctgacgacat catcgccgcc ttcgcccagc acggtatcgt tgtcggagcc
    12061 gccggagatc agatcgtccc agcttgtgcc ggtgatgaag tctttcccgg ccccgcccgt
    12121 cgcattgagg gaactgagcg gaaatgatcc ggaaagggag atcgtgtcgt caccggaggc
    12181 gccagtcttc ttgacgctgc cgtaaggtgc cgttgccatg aagtcctcct gttggctgaa
    12241 tgccttgaag agaggtttag gcggctcgta aaattgtagc ggtgcagtgc cgcacggagc
    12301 gatcgcgcgg cgaaccgctc aaaaatgccc gaaggacggc tttccgaaac tcaggtcttc
    12361 gccggcgaac ccgtggatgc gcacgccgcg cgccgtcagc cgtccgatgg gcgtcaccgg
    12421 aagcgcgatg tcctgcgccg cacgttcgaa cgccgatgcg cggtccggcg gtacggcggc
    12481 cagcacctgg taatcgtcgc cgcccgtgag aaccgcatcc agcaggaagc gatggcgcgc
    12541 caccagcgta ggcaacggcg tgccggggag aggcaagggc acgcgcgccg cctcgatctc
    12601 gagccctgtt ccgctcgcct tcgccagctt cagcgcatcg ccgatcagac cgtcggagac
    12661 atccatcgcg gcgctcgcgt ggagccggat gccttcggcg agtgctaccg gtggctgggg
    12721 aacatgaaag gccgagatca gcgctgcgcg ggcgtcgcca gacaaggggc gagaccacgg
    12781 cggatcgcca tcgcggatga ggtgaagccc gagcgccgcc gcgccgatga cgccggagac
    12841 gtagagcaga tcgcccggct ttccgcccga acgccggacc atggttcccg ccggcatgct
    12901 tccaagcgcg gtgatcgaga ggatcaaagg cccgcccgcc ctcaccgtat cgccgccgag
    12961 caacgggcag ccgaagagcg ccgaatccgc cgcaagaccg ctcgcgaaag tcgccagcca
    13021 gtcctcgcgc cagtccgttg gcaggccaag gcccagcagg aagccgagcg ggcgcgcgcc
    13081 tttcgccgcc agatcggaca ggttcacgcg caaagccttg cgggcgatcg tttccggcgg
    13141 atcgttcggg aagaaatgca cgccggcgac cagcgcgtcc ttggtcagca cgagatcgca
    13201 gccgggcggt ggcgtcagca gcgcggcgtc gtcgacgaga ccgaggccgc cagcgccggc
    13261 catcggcgcg aagtagcggg cgatgaggtc gaactcgccg gggcgctcac cgggactcac
    13321 ggattgaacc ctgcttgcgc atccgcccta tcccctggcg agttcctccg cccgcagttc
    13381 gcgggccaga cggtcgagca cggcgttgac catgcccacc tcatccttgt cgagaaaggc
    13441 cgaggcgatg tcgacatatt cggataccac gaccttcacc ggcacatccg gcgttttcat
    13501 cagctcgtag gcgccgccgc gcagactggc gcgcatcagc gcctcgaccc gtttcagcgg
    13561 ccagccgtcg gagaggatgc ggtcaaccgc ccgatcgatc gcgacctgat gctcgatcac
    13621 gccctgcacg atctggcgga acagactgcg ctcggccggc ttgtactggt cgccctcgat
    13681 ctcctggccg atccagaagc gctcgaactc ggaaaagacc tcgatcacgc ccttgccggt
    13741 caggtccatc tggtagagcg cctgtaccgc ggagagccga gcggccgaac gtttttcccc
    13801 gcgggtcatc gcggcacccc gcgccgcttg agcgccgcca gcgccagcgc cg
//
LOCUS       DAOMXN010000040        13840 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_2430_length_13840_cov_13.529416, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000040 DAOMXN010000000
VERSION     DAOMXN010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 13840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13840
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2430_length_13840_cov_13.529416"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..7776)
                     /locus_tag="PK812_05130"
     CDS             complement(<1..7776)
                     /locus_tag="PK812_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68973.1"
                     /translation="MTALLLSLHAEAAAQDRAQNASDELFPSKPADNQRRRTAPAEAG
                     RQPATPARSATEQGTQPGPAQPQRPATAANQPKKKVDESGLRYFASQNQPKRVEKEIQ
                     RLKALYPDWSPPDDLYLGPPGGPDEQPFWDLFGADKLEELRAKIAERMKSEPGWKPSA
                     ALMYKIERKESRNKLVAAYQAKEWQKVIEVAAADPKIMVCADIDVPWRVADAFAETGQ
                     KKQTFDVYRFILNNCRDKAFRITTLKKALGYFEPDEVDVLIAMGTRNEDGTHEFDDVR
                     LDILRVRLGRMAEGKAEPRVQPQDIKFFEDQTKTHARSADASLLGWHRFASKDWEASI
                     AWFKLALEWPLQLREDGSVMAEKAKMAEGHILALKGLGKFEEAEALAFQWRDKSETIR
                     DLFVDIFGDALLRSPASTVFAPERITRYEMLVASAQSVFGTQALGWYHFSRSRWDEAV
                     RWFQASYDWSDKEKLDTKTIEGLALALRSAARREEAEDLAFEWRDRSKLLRTIYVETF
                     AEALVQQSPPIAFSDTRIDRYAALVMADGSAMGAQGLAWYSYDRKRWTEAARWFKASI
                     DWSPDGANDAKLAEGLAQSLRFAGRYEEAEDVAYEWRERSPAMKVLFVEITAEHLGKI
                     DPARPYPSARLNRFAQIVQSERSSFGAQAIAWHLYDRKTFIEAARWFRTALTFNPAET
                     TAVKVLEGYVLSVRYQGEVREAENLAYDWRDRSESLRQIYFEIAAELISTIKPPAVYP
                     ADRMERFIELTERAKSAFGAQAIGWFHHQREDYATAAKWFNQSLQWGGDTSDPRTAQG
                     FLLALISLGRLEEADRLAFSWHEKSEEFRTLYIDVFGGAILRATPPQAFPEGLLARFA
                     YISGSAKSVFGSQAVAWYYYDRQNWAESARWFQASLDWAGEGKHDPKTVEGFAQSLRY
                     LGRREEAEEILYAWRDKVPILRKLYVEIFSEALVSTSAPIFFSDERLNRYGALVLDDK
                     DWFGAQGLAWYRHDRRQWPDAVRWFKYAREWSVDGKGDAKMAEGLAFSLRNLELYDES
                     EFVSVEWKDKSPQMRKLYSETVGEFLVRLKNDVTYPDERMHRYLETVAADRNNIGAQS
                     AGWYYFARDMYDPAAQWFLASLQWGPDYVRDPKTAEGYVTALRLGGRLMDAEAAAFEL
                     RDKSEQLRQLYFDIAGEAIANLKPPETYPADRLARFADLAAEARSAIGAQSIAWYFQA
                     RENWREATAWFEKALEWGIDVKDAKTAQGYLIALVGVGRYEDADRLAALWHEKSEEFR
                     TLYIDVFGGAILRATPPQAFPEGLLARFAYISGSAKSVFGSQAVAWYYYDRQNWAESA
                     RWFQASLDWAGEGKHDPKTVEGFAQSLRYLGRREEAEEILYAWRDKVPILRKLYVEIF
                     SEALVSTSAPIFFSDERLNRYGALVLDDKDWFGAQGLAWYRHDRRQWPDAVRWFKYAR
                     EWSVDGKGDAKMAEGLAFSLRNLELYDESEFVSVEWKDKSPQMRKLYSETVGEFLVRL
                     KNDVTYPDERMHRYLETVAADRNNIGAQSAGWYYFARDMYDPAAQWFLASLQWGPDYV
                     RDPKTAEGYVTALRLGGRLMDAEAAAFELRDKSEQLRQLYFDIAGEAIANLKPPETYP
                     ADRLARFADLAAEARSAIGAQSIAWYFQARENWREAAFWFEKSIQWGGEAEDPKSAEG
                     YVVALIGLGRYEEAEALVVKWRSRSDRIAATFLEVMGSQLLRLPATATLPEERLARYG
                     VLVGSERSLFGSQALAWYFYERKQWDDAARWFRANLDWTPQGQIDPKSIEGYAQSLRS
                     AGRRDDAEELLFLWRDSNPTLRRLYLETFAEALVNQSAPLMFEDERIDRYADLVLNDR
                     SSFGAQALAWYRYNRRVWHDAARWFKHARQWTQDGKGDAKMAEGLAFSLRNMDLYDES
                     EAVSWDWRDRSPVVRKLYVETVAEYIVRLWPRETYPVDRLSRYQIVVDQDKSSIGAQA
                     AGWYYFARDRFGEAAPWFRKALDWGPDPIRDPKTAEGLAIALRNSGQIDEAESFAFAW
                     RDRSDRLREMYFEFSAQAMARIRPPQHYPVDRMTRFISLTNASRNAFGAQAIGWYKAE
                     RKEWADAVKWFKQALDWTGDDKDAKTIEGYAQALRNVGRIDEAETLAFMWKDRSEGMA
                     DLYLQIVTDIISKLKPTDTYPADRLDKYIQLVSDRQSVAGAQTIGWYRLQRKDFADGE
                     KWFKLALDWLGEAKDPKVIEGYAAALRQQKKLEDAELLAWEWRDRNDGLRDLYLQVAM
                     ERLVAIKPPAVLPADRLKNLIDFTRAGKSASGALALGWYHYDRKDWASALEWFKLATE
                     WETDPKNSKGAEGYALTLKNLGKIEEAEAYAYEWRDKSDAMRALYIDSVPEMLGKLGP
                     TGTFPVERLKRYAALVTLAKSAYGAQALAWYWHERKDYAQAAVWFKSSLDWSGDAKDA
                     KTAEGYVLALRSAGRLDEAELIAYEWRERADTLRTLYIESFAEALTRTNPPPPFPPDR
                     LARYAAIVSADKSAIGAQALGWYSHNIKQFRPARAWFEKAMQWGPQEGSALGLALSAR
                     SMNDRAGYEQIVNMYRGTYPKLLELLAPQAATGTQPYATATQPATGTRVIEVIR"
     gene            complement(7788..8879)
                     /locus_tag="PK812_05135"
     CDS             complement(7788..8879)
                     /locus_tag="PK812_05135"
                     /inference="COORDINATES: protein motif:HMM:NF013438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HRJ68974.1"
                     /translation="MRRSLAALAAALACWLALAVHNGVAANQPAPIRSLKAEPDAWPA
                     GTWAGYREKYIMPEGRVVDDGNNGISHSEGQGYGMLLAVIADDQATFNRLWTWTRREL
                     YVRPDGLAAWKWEPDKTPRVTDPNNASDGDLLIAWALAEAAERWGKRELATEAEKIAR
                     AIDRVNGARSPFGRVLMPGAVGFSATDRPDGPVVNPSYWVFPAFDSLRRIAPQIDWPG
                     YRASGLTLLRHARFGQHKLPPDWLSIAGSKAAVAEGFPPTFSYNAVRIPLYLAWGGVS
                     APEMHAPYLSLLKASAGNLTEADLKKDAPGEPMGDAGYLAIFALSECVVNQSQIPHGL
                     RSVSFDRYYSATLHMLALVAVRLRYGGCW"
     gene            complement(8876..11341)
                     /locus_tag="PK812_05140"
     CDS             complement(8876..11341)
                     /locus_tag="PK812_05140"
                     /inference="COORDINATES: protein motif:HMM:NF015149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HRJ68975.1"
                     /translation="MRRCFIPVLTLAAVALTGATAALSQTAGMPSRLPDRSLVTGSIS
                     APGQNYFQLNSGPSASSIATQVLQRFPANGRQLRFENEVGWLDLPFYVRPAQIEDSAR
                     LRLRYVNSVAVMPEASRLIVYVNDQPIAETNIEASSEPATVDVPIPAGVFEAGYNSVR
                     ISVQQRHRVDCTIQATNELWTQIDPVASGIAFTTTNDQLTRTEDLLGLPVDEDGATPI
                     FVNIASGADAAIMDRSLRAAQALAVRLDLRRPRIAFSRELTKAPGIQIIVGTIAELRS
                     RGIEINPLGDSGVQVAGRMAETVRVTISGASAADVDESIDRLAAEKPADGERGTRAGL
                     RALAAARGFPLNSEGAVSLRDIGFASQDFGGRVFRSQFNIVLPSDFYPADNGKATLML
                     NAGYAAGLLTTNEVRVHVNGKIVGARRLKKSYGESMRQRALEMSLKTLQPGFNSIMIE
                     IHSEAESDRDCNPLSLLESSKRLVVSDQTAFTIPRIARVAHLPSMSATAATGFPLADT
                     KEPLLLYMPKADFASLGAAGTLLARAAVAAGKPIATQISLTAPEKSGGNALIVGTAAD
                     TPAEIARWFGLPKEGLPASWIKRAAPRAGEARREGAAQPGVAPSERNAPAKGPRAALD
                     SDEDLTTATAASDAKGGKAPSAPDLLDQMEDSISQDSILAGFLRGLANALQRNIGYSA
                     DQLSFLNGGREPFAVSQNARLLVAQAPAPGANGGTWLLLMAQDSATLARDTAMLVAPT
                     VWNQLGGRVSTLDTKDAEVSVWQTNSNYYYRLDDKSLPNITLFAAGWLSNNILYYVLV
                     ILTICGMFGIVSRLVLNRIGTRP"
     gene            complement(11355..13628)
                     /gene="bcsA"
                     /locus_tag="PK812_05145"
     CDS             complement(11355..13628)
                     /gene="bcsA"
                     /locus_tag="PK812_05145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016759 - cellulose synthase activity
                     [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HRJ68976.1"
                     /translation="MTTWPISTQAQFTLGLAVILVMTLIRKFRLGGVWRPIFLSLGIT
                     MILRYVYWRSTSTLPPMNDWVNFVPGFTLYLAEMYCVMMFFISLFIIADPIERKPPPQ
                     LPDDRLPSVDVYIPTYNEGSDLLAATVAAATQMDYPKDKLNIYLLDDGGTDQKCNQAD
                     PEKAQEARDRRVELQALCAELGITYITRARNEHAKAGNMNNALQQTKGQIIVVFDADH
                     APARQFLRETVGYFATDRKLFLVQTPHFFINPDPIEKNLQTFERMPSENEMFYGTIQK
                     GLDKWNGAFFCGSAALIARRALESVGGFSGISITEDCETALDLHTRGWNSLYVDKPLI
                     AGLQPESLVSFIGQRSRWCRGMIQILVLKNPLLKKNISLKQRIAYVSSMFYWLFPIPR
                     VIFLMSPFLYIFFNMQIFVANAQEFFAYITIYMLTHMTIQNYLYGDTRWPWISEAYEY
                     VQSIYLVKGIWGVLINPRKPTFNVTAKGLTLDKDHLSELAWPYFATFALILTSLIVAC
                     YRFLSDNEMNELLLIVGGWSFYNLIIAGVALGIVAERRERRKTPRLKIVRKGEITANG
                     VSVPVEIKDCSIGGMRVRPLVGKLPAEFSRGGQCLVHVAPNREGFDTAPIPCILRNVS
                     NDGSGAIYGLQFSGLSPSHYRGIADAMYADMQVFENFRSSRRKIKGITMGTLQFFWWS
                     INHTLRAFYYAVALRKTSSEPAVEANAPAETSAPTADEVLSQSQHQTQPHGAAEPALQ
                     PSHEPQMVGIRQMAHAH"
     gene            13549..13725
                     /locus_tag="PK812_05150"
     CDS             13549..13725
                     /locus_tag="PK812_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68977.1"
                     /translation="MRISVITRITANPSVNWACVLIGQVVRPMMVAASANHPATEEYF
                     VSFDPPEDVATGAS"
BASE COUNT         2330 a         4382 c         4654 g         2474 t
ORIGIN      
        1 gcggatcacc tcgatcacgc gcgttccggt cgcgggctgc gtcgccgtcg cgtagggctg
       61 cgtgcctgtc gccgcctgag gcgccagcag ctccagcagc ttgggatacg tgccgcggta
      121 catgttgacg atctgctcgt agccagcacg gtcgttcatc gagcgggccg acagggcaag
      181 gcctaacgcc gagccctctt gcgggcccca ttgcatcgcc ttctcgaacc aggcccgcgc
      241 cgggcggaac tgcttgatgt tgtgggaata ccagccgagc gcctgggcgc cgatggcact
      301 cttgtcggcc gacacgatgg cggcgtaacg cgccagccgg tccggcggga agggcggcgg
      361 cgggttggtg cgggtcagag cctcggcgaa gctctcgatg tagagcgtgc gcagcgtgtc
      421 ggcccgctcg cgccactcgt aggcgatgag ttcggcctca tccaggcggc cggccgagcg
      481 tagcgccagc acgtagccct cggcggtctt ggcatccttg gcgtcgcccg accagtcgag
      541 agaggacttg aaccagacag cggcctgcgc gtagtccttg cgctcatgcc agtaccaggc
      601 gagcgcctgg gcgccgtagg cggacttggc gagcgtcacc agggcggcat agcgcttgag
      661 gcgctcgacc gggaacgtac cggtcggccc cagcttgccg agcatctcgg gcacggagtc
      721 gatgtagagt gcgcgcatgg cgtcgctctt gtcgcgccat tcgtaggcat aggcctccgc
      781 ctcctcgatc ttgccgaggt tcttcagcgt cagcgcatag ccttccgcac ccttggagtt
      841 cttggggtcg gtctcccact ccgtggccag cttgaaccac tccagcgcgc tggcccagtc
      901 cttgcggtcg tagtgatacc agccgagggc cagcgctccc gatgccgact ttccggcacg
      961 ggtaaagtcg atcaggttct tcagcctgtc cgccggcagc acggcagggg gcttgatcgc
     1021 gacaagccgt tccatcgcga cctgaaggta gagatcgcgc aggccgtcgt tgcggtcgcg
     1081 ccattcccag gcgagaagct cggcgtcttc cagtttcttc tgctggcgca gggctgcggc
     1141 atagccctcg atgaccttag gatccttggc ttcaccgagc cagtccagcg ccagcttgaa
     1201 ccatttctcg ccgtcggcga agtccttgcg ctgcaggcgg taccagccga tggtctgggc
     1261 gccggcgacc gactggcgat cgctgaccag ctggatgtac ttgtcgagcc ggtcggccgg
     1321 gtaagtgtcg gtcggcttca gcttggagat gatgtcggtg acgatctgca gatacagatc
     1381 ggccatgccc tcgctgcggt ccttccacat gaaggcgagc gtctcggcct cgtcgatgcg
     1441 gccgacgttg cgcagggcct gggcatagcc ttcgatggtc ttggcgtcct tgtcgtcgcc
     1501 ggtccagtcg agcgcctgct tgaaccactt gaccgcgtcc gcccattcct tgcgctcggc
     1561 cttgtaccag ccgatcgcct gggcgccgaa ggcattcctg gaggcgttgg tcaacgagat
     1621 gaagcgggtc atccggtcga ccgggtagtg ctgcggcggg cggatgcgcg ccatggcctg
     1681 cgcggagaat tcgaagtaca tctcgcgcaa tcggtcgctg cgatcgcgcc aggcgaaggc
     1741 gaagctctcg gcctcgtcga tctgtccgct gttacgcagg gcgatcgcca gtccttctgc
     1801 ggtcttggga tcgcggatcg ggtccggacc ccagtcgagc gccttgcgga accagggcgc
     1861 ggcctcgccg aagcggtcgc gggcgaagta gtaccatccg gccgcctgcg cgccgatgga
     1921 agatttgtcc tggtcgacta cgatctggta acgtgacagg cgatcgaccg gatacgtctc
     1981 tcgcggccag aggcgcacga tgtattccgc gacggtttcc acatagagct tgcgcaccac
     2041 agggctgcgg tcgcgccagt cccacgagac cgcttccgat tcgtcgtaga ggtccatgtt
     2101 gcgcagcgag aaggcaaggc cttccgccat cttggcgtcg cccttgccgt cctgcgtcca
     2161 ttgccgcgcg tgcttgaacc agcgcgccgc atcgtgccag acacggcggt tgtagcggta
     2221 ccaggccagc gcctgtgcgc cgaaggacga gcggtcgttc agtaccaggt cggcgtagcg
     2281 gtcgatgcgc tcatcctcga acatcagggg agcgctctgg ttgaccaggg cttcggcgaa
     2341 ggtctccagg taaagccggc gcagtgtggg gttggaatcg cgccacagga acagcagttc
     2401 ctccgcgtcg tcccggcggc cggcggaccg cagcgactgc gcatagcctt cgatgctctt
     2461 cggatcgatc tgtccctgcg gtgtccagtc aagattggcg cggaaccaac gcgcggcgtc
     2521 gtcccactgc ttgcgctcgt agaagtacca ggccagggcc tgcgagccga acagggagcg
     2581 ctctgacccc acaagcacgc cgtaacgcgc cagccgttcc tcgggcagcg tggcggtggc
     2641 tggcaggcgc agcagctggc tgcccatgac ttcaaggaag gtcgcggcga tgcggtccga
     2701 acggctgcgc cacttgacca ccagcgcctc ggcttcctcg tagcggccaa ggccaatcaa
     2761 cgccacgaca tagccctcgg cgctcttggg gtcctcggct tcgccgcccc actggatcga
     2821 tttctcgaac cagaacgcgg cctctcgcca gttttcgcgg gcctggaagt accaggcgat
     2881 ggactgggcg ccgatggcgg acctggcctc ggcggcgagg tcggcgaagc gggcgaggcg
     2941 atcggccgga taggtctccg gcggcttgag gttggcgatg gcctcgccgg cgatgtcgaa
     3001 gtaaagctgg cgcagctgct cggacttgtc gcgcagctcg aaggcggcgg cctccgcgtc
     3061 catcagccgg ccgccgagcc gcagcgccgt gacgtagcct tcggcggtct tggggtcgcg
     3121 gacgtaatcc ggcccccatt gcaggctggc caggaaccac tgggccgccg gatcgtacat
     3181 gtcgcgggcg aagtagtacc agccggccga ttgcgcgccg atgttgttgc ggtccgccgc
     3241 caccgtctcc agatagcggt gcatgcgctc gtcggggtag gtgacgtcgt tcttcagacg
     3301 caccaggaac tcgcccaccg tctcggagta gagcttgcgc atctgcgggc tcttgtcctt
     3361 ccactcgacg gagacgaact cggactcgtc gtaaagctcg aggttgcgca gggagaaggc
     3421 caggccttcc gccatcttcg catcgccctt gccgtcgacc gaccattcgc gggcgtactt
     3481 gaaccagcgc accgcgtccg gccactggcg gcggtcgtgg cggtaccagg ccaggccctg
     3541 cgcgccgaac cagtccttgt cgtcaagaac gagcgcgccg tagcggttca gccgctcgtc
     3601 ggagaagaag atcggggcgg aggtggagac cagcgcctcg gagaagatct cgacatagag
     3661 cttgcgcagg atcgggacct tgtcgcgcca ggcgtagagg atttcctccg cctcctcgcg
     3721 gcggccgaga tagcgcagcg actgggcaaa gccctcgacg gtcttgggat cgtgcttgcc
     3781 ctcgcccgcc cagtcgagcg aggcctggaa ccagcgggcg gattcagccc agttctggcg
     3841 gtcgtagtaa taccaggcca cggcctgcga gccgaacacc gacttcgccg aaccggagat
     3901 gtaggcaaac cgcgccagca gaccttccgg aaacgcctgc ggaggcgtcg cccgcagaat
     3961 cgcgccgccg aatacgtcaa tgtacagcgt ccggaactcc tcagacttct catgccagag
     4021 ggcggccaga cggtcggcat cctcgtaacg gccgacgccg acgagggcga tgaggtagcc
     4081 ctgcgccgtc ttggcatcct tgacgtcgat gccccattcc agcgccttct cgaaccaggc
     4141 ggtcgcctct cgccagtttt cgcgggcctg gaagtaccag gcgatggact gggcgccgat
     4201 ggcggacctg gcctcggcgg cgaggtcggc gaagcgggcg aggcgatcgg ccggataggt
     4261 ctccggcggc ttgaggttgg cgatggcctc gccggcgatg tcgaagtaaa gctggcgcag
     4321 ctgctcggac ttgtcgcgca gctcgaaggc ggcggcctcc gcgtccatca gccggccgcc
     4381 gagccgcagc gccgtgacgt agccttcggc ggtcttgggg tcgcggacgt aatccggccc
     4441 ccattgcagg ctggccagga accactgggc cgccggatcg tacatgtcgc gggcgaagta
     4501 gtaccagccg gccgattgcg cgccgatgtt gttgcggtcc gccgccaccg tctccagata
     4561 gcggtgcatg cgctcgtcgg ggtaggtgac gtcgttcttc agacgcacca ggaactcgcc
     4621 caccgtctcg gagtagagct tgcgcatctg cgggctcttg tccttccact cgacggagac
     4681 gaactcggac tcgtcgtaaa gctcgaggtt gcgcagggag aaggccaggc cttccgccat
     4741 cttcgcatcg cccttgccgt cgaccgacca ttcgcgggcg tacttgaacc agcgcaccgc
     4801 gtccggccac tggcggcggt cgtggcggta ccaggccagg ccctgcgcgc cgaaccagtc
     4861 cttgtcgtca agaacgagcg cgccgtagcg gttcagccgc tcgtcggaga agaagatcgg
     4921 ggcggaggtg gagaccagcg cctcggagaa gatctcgaca tagagcttgc gcaggatcgg
     4981 gaccttgtcg cgccaggcgt agaggatttc ctccgcctcc tcgcggcggc cgagatagcg
     5041 cagcgactgg gcaaagccct cgacggtctt gggatcgtgc ttgccctcgc ccgcccagtc
     5101 gagcgaggcc tggaaccagc gggcggattc agcccagttc tggcggtcgt agtaatacca
     5161 ggccacggcc tgcgagccga acaccgactt cgccgaaccg gagatgtagg caaaccgcgc
     5221 cagcagacct tccggaaacg cctgcggagg cgtcgcccgc agaatcgcgc cgccgaatac
     5281 gtcaatgtac agcgtccgga actcctcaga cttctcatgc caggagaagg cgaggcgatc
     5341 cgcctcctca agccgtccga ggctgatcag cgccagcagg aaaccctgcg cggtgcgggg
     5401 atcggacgtg tcgccgcccc attgcaggct ctggttgaac catttggccg cggtcgcata
     5461 gtcctcgcgc tggtggtgga accagccgat ggcctgcgcg ccgaaggcgg attttgcccg
     5521 ctccgtcagc tcgatgaagc gctccatccg atcggccgga tagaccgctg gcggcttgat
     5581 ggtcgagatc agttccgcag cgatctcgaa atagatctgg cgcaaggact cggagcggtc
     5641 gcgccagtca taggcgaggt tctccgcttc ccgcacctcg ccctgatagc gcaccgacag
     5701 tacgtagcct tccagcacct tcaccgccgt cgtctcggcc gggttgaagg tcagcgcagt
     5761 gcggaaccag cgcgcggcct cgatgaaggt cttgcggtca tacagatgcc aggcgattgc
     5821 ctgcgcgccg aagctcgagc gttcggactg gacgatctgc gcgaaacggt tgaggcgggc
     5881 gctcgggtag gggcgcgccg gatcgatctt gcccaggtgc tcggcggtga tctcgacgaa
     5941 cagcactttc atggcggggc tgcgctcgcg ccactcgtag gcgacgtctt ccgcctcctc
     6001 gtagcggccg gcgaagcgca gggactgggc caggccttcc gccagcttgg cgtcgttggc
     6061 gccgtcgggc gaccagtcaa tggaggcctt gaaccagcgc gccgcctcgg tccagcgctt
     6121 gcggtcgtag gaataccagg ccagaccctg ggcgcccatg gccgagccat ccgccatgac
     6181 cagtgcggcg taccggtcga tccgtgtgtc ggagaaggcg atcggcgggc tttgctgcac
     6241 cagcgcctcg gcgaaggtct cgacatagat ggtgcgcagg agcttggagc gatcacgcca
     6301 ctcgaaggca aggtcctctg cctcttcgcg gcgcgctgcg ctgcgcaacg cgagggcaag
     6361 gccttcgatc gtcttggtgt cgagcttttc cttgtccgac cagtcgtagc tggcctggaa
     6421 ccagcgcacc gcttcgtccc accgcgaccg ggagaagtga taccagccaa gcgcctgcgt
     6481 cccgaagacc gactgtgcgg aagccaccag catctcgtag cgcgtgatgc gctccggcgc
     6541 gaacacggtc gaggcgggcg agcgcaacaa tgcatcgccg aagatgtcga cgaacaggtc
     6601 gcggatggtc tcgctcttgt cacgccactg gaaggccagt gcctcggctt cctcgaactt
     6661 gccaagcccc ttcagcgcga ggatatggcc ttccgccatc ttggcctttt cggccatgac
     6721 actgccgtct tcccgcagct gcagcggcca ttccagcgcg agcttgaacc aggcgatcga
     6781 agcttcccag tccttgctgg cgaagcgatg ccagccaagc agggacgcat ccgccgaacg
     6841 tgcgtgcgtc ttcgtctggt cctcgaagaa cttgatgtcc tgcggctgga cgcgcggctc
     6901 tgccttgcct tcggccatgc gcccgaggcg cacgcgcagg atgtcgaggc gaacgtcgtc
     6961 gaactcgtgg gttccgtcct cgttgcgggt ccccatcgcg atgagaacgt ccacctcgtc
     7021 cggttcgaaa tacccaaggg ccttcttcag cgtggtaatg cggaaggcct tgtcgcggca
     7081 gttgttcagg atgaagcggt agacgtcgaa ggtctgcttc ttctgtcctg tttccgcgaa
     7141 ggcatccgcc actcgccagg ggacatcgat gtcggcgcag accatgatct tcggatcggc
     7201 tgcggcgacc tcgatgacct tctgccattc cttggcctga taggcggcga cgagcttgtt
     7261 gcgcgattcc ttgcgctcga tcttgtacat cagcgcggcg gacggcttcc agcccggctc
     7321 ggacttcatc cgctcggcga tcttggcccg cagttcctcc agcttgtccg caccaaaaag
     7381 gtcccagaac ggctgttcat caggaccgcc cggtggcccg agatagagat cgtcgggcgg
     7441 gctccagtca ggatagagcg ccttgagacg ctggatttcc ttctcgacac gcttgggctg
     7501 gttctgcgag gcgaagtagc gcagcccgct ttcatccacc ttcttcttcg gctgattcgc
     7561 agccgtcgcg ggacgctgcg gctgagccgg accgggctgg gttccctgtt cggtggcgga
     7621 gcgggcggga gtcgcgggct gacggcccgc ttcagccgga gccgtcctgc ggcgctggtt
     7681 gtctgcgggc ttgctcggaa agagttcgtc ggaggcgttt tgggcacgat cctgcgccgc
     7741 cgcttccgca tggagcgaaa gcagcaaagc tgtcaagagc gaggccctta ccaacatcca
     7801 ccatacctaa gacgcaccgc gacgagtgcg agcatgtgca acgtcgcgga atagtaccgg
     7861 tcgaaactga cggaacgtaa accatggggg atttgcgatt gattaacgac acattccgac
     7921 aaagcaaaaa ttgcgaggta gcccgcgtcg cccatgggct cgccgggggc atcctttttc
     7981 aggtcagctt ccgtcagatt gcccgcagag gctttcagca gcgacagata gggggcatgc
     8041 atttcaggcg ccgaaacgcc tccccaggcc aggtacagcg gaatgcgcac ggcattgtag
     8101 gaaaatgtcg gcgggaaccc ctcggctacc gcggccttgc tgccggcaat ggagagccag
     8161 tccggcggca gcttgtgctg accgaagcgg gcgtgccgca gaagtgtcag accgctcgcg
     8221 cgatacccgg gccagtcgat ctgcggcgcg atgcgccgga gcgaatcaaa ggccgggaag
     8281 acccagtagg aaggattgac caccggaccg tccggccggt cggtcgccga gaaccccacg
     8341 gcgcccggca tcagcacgcg gccgaagggg ctgcgcgcgc cgttgacccg gtcgatcgcc
     8401 cgcgcgattt tctccgcctc ggtggcgagc tcgcgcttgc cccagcgttc ggcggcctcg
     8461 gcgagtgccc aggcgatcag caggtcgccg tcagacgcgt tgttcggatc ggtaacgcgg
     8521 ggagtcttgt ccggctccca tttccaggcg gcgagcccgt ctggccggac gtagagttcg
     8581 cgcctagtcc aggtccagag ccggttgaac gtcgcctgat cgtcagcgat cacggccagc
     8641 agcatgccgt agccctggcc ttccgagtgg ctgatgccgt tgttgccgtc gtccacgacg
     8701 cggccctccg gcatgatgta tttttccctg tagccggccc aggttccggc gggccaggcg
     8761 tcaggctcgg ctttcaggga gcggatcggc gccggctggt tggcggcaac cccgttgtgg
     8821 acggcaagcg caagccagca ggcgagcgcc gcggcaagcg cagcaaggga ccgtctcatg
     8881 gccgggtgcc gatccggttc agaacgagac gggagacgat gccgaacatc ccgcagatcg
     8941 tcaggatgac aagcacgtag tagagaatgt tgttggacag ccagcctgcg gcgaacaacg
     9001 tgatgttcgg cagggacttg tcgtcgaggc ggtagtagta gttggagttc gtctgccaga
     9061 ccgacacttc agcatccttg gtgtcgagcg tcgaaacccg accgcccaac tggttccaaa
     9121 ccgtcggcgc caccagcatg gcggtgtcgc gcgccaaggt cgcggaatcc tgagccatca
     9181 gcagcagcca ggtgccgcca ttggccccag gagccggagc ctgcgcgacc agcaggcgcg
     9241 cattctgcga tacggcgaaa ggctcacgcc cgccgttcag aaacgaaagc tgatccgcgc
     9301 tgtagccgat gttgcgctgc agggcattgg ccagaccccg caggaagccg gccaggatgc
     9361 tgtcctggct gatgctgtct tccatctggt ccaggagatc cggcgcgctg ggggccttgc
     9421 ctcccttcgc gtcgctggct gccgtcgccg tggtcaggtc ctcgtcgctg tccagcgccg
     9481 cgcgcgggcc cttggcgggt gcgttgcgct ccgaaggggc gacgccgggc tgggcggctc
     9541 cttccctgcg cgcctctccg gccctgggcg cagcgcgctt gatccagctt gccggcagcc
     9601 cttccttggg caggccgaac cagcgggcga tctccgccgg cgtgtctgcc gccgttccga
     9661 cgatgagcgc gttgcctccg gacttttccg gcgccgtcag cgaaatctgg gtggcaatcg
     9721 gcttgccagc cgcgacggcg gccctggcca gcagggtgcc ggcggcgccc agagacgcga
     9781 agtccgcctt gggcatgtaa aggagcaggg gctccttcgt gtcggcaagc gggaacccgg
     9841 tcgccgcggt cgcgctcatg ctcggcagat gcgcgacccg cgcgatgcgt ggaatggtga
     9901 aggcggtctg gtccgaaacg accagtctct tgctggattc gagcagggaa agcgggttgc
     9961 agtcccggtc gctttccgcc tccgaatgga tctcgatcat gatcgagttg aagcccggct
    10021 gaagggtctt cagcgacatt tcgagcgcgc gctggcgcat gctctcgccg taactcttct
    10081 tcagccggcg cgcgccgacg atcttgccgt tgacgtgcac ccgaacctcg ttggtcgtca
    10141 acagtccggc ggcatagccg gcgttcagca tcaacgtggc cttgccattg tcggcgggat
    10201 aaaagtcgga cggcaggacg atgttgaact gcgagcggaa cacgcgcccg ccgaagtcct
    10261 gcgatgcaaa gccgatgtca cgcagggaaa cggcgccttc cgagttcagc ggaaaacctc
    10321 gggccgccgc cagcgcgcgc aatccggccc gggttccgcg ctctccgtcg gccggctttt
    10381 cggccgccag ccggtcgatg ctctcatcga catcggccgc cgaggcgccg gatatggtga
    10441 cgcgcacggt ctccgccatg cggccggcca cctgcacgcc ggagtcgccg agcggattga
    10501 tctcgatgcc gcgcgagcgg agctcggcga tggtgccgac aatgatctgg atgcccggcg
    10561 ccttggtcag ttcacggctg aaggcgatgc gcgggcggcg cagatcaagc cggaccgcaa
    10621 gcgcctgcgc cgcccgcaac gagcgatcca tgatggccgc gtcagcgccc gaagcgatgt
    10681 tgacgaagat gggcgtcgca ccgtcttcgt cgaccggcag gccgaggagg tcttccgtgc
    10741 gcgtcagctg atcgttcgtc gtggtgaagg cgatgccgga agcgaccggg tcgatctggg
    10801 tccacagttc gttcgtcgcc tggatcgtgc agtcgacgcg atggcgctgt tgaacgctga
    10861 tccgcaccga attgtagccg gcttcgaaaa ctccggcggg aataggaaca tctacggtcg
    10921 ccggttcgga cgaagcttcg atgttggtct cggcgatggg ctgatcgttg acgtagacaa
    10981 tgagtctgga ggcctccggc atcacggcga ccgaattcac ataacgcaga cgcagacgcg
    11041 ccgaatcctc gatctgagcc ggccgcacgt agaacggcag atcgagccag ccgacctcgt
    11101 tctcgaaccg cagctggcgc ccgttcgccg ggaatctctg aagaacctgt gtcgcgatgg
    11161 acgaggcact cgggccggaa ttgagttgaa aatagttctg gccgggtgcc gaaatcgatc
    11221 cggtgacgag actcctgtcg gggagccggc tcggcatgcc cgccgtttgt gacagtgcgg
    11281 ccgtcgcgcc ggtcagcgcg acagctgcaa gcgtcaggac ggggatgaag cagcgacgca
    11341 tcggccaggc cttcctagtg tgcgtgcgcc atctgccgga tgccgaccat ctgcggctcg
    11401 tgcgacggct gaagggctgg ttcggctgcg ccgtgcggct gggtctggtg ctgagactgg
    11461 ctcaggacct cgtccgccgt gggcgcactg gtttcggcgg gcgcgttggc ttcgaccgcc
    11521 ggttccgaag atgtcttgcg cagggcgacg gcatagtaga aggcgcgcag cgtatggttg
    11581 atgctccacc agaagaactg gagcgtcccc atggtgatgc ccttgatctt ccgccggctg
    11641 gagcggaagt tctcgaagac ctgcatgtcg gcgtacatcg cgtcggcgat gccgcggtag
    11701 tgcgatgggc tcaggccgct gaattgcaag ccgtagatcg cgccgctgcc gtcgttggag
    11761 acattgcgca ggatgcaggg gatcggcgcc gtgtcgaaac cctcacggtt cggcgcgaca
    11821 tgcacgaggc actgcccgcc gcggctgaac tcggctggca gcttgcctac gagcgggcgc
    11881 acgcgcatgc cgccgatcga gcaatccttg atctctaccg gcacgctcac gccgttggcg
    11941 gtgatctcgc ccttgcgcac gatcttcagg cgcggggtct tgcggcgttc gcggcgctcg
    12001 gcgacgatgc cgagggccac gccggcgatg atcaggttgt agaagctcca gccgccgacg
    12061 atcagcagca gctcattcat ttcgttgtcc gaaaggaagc ggtagcaggc gacgatcagg
    12121 ctggtcagga tcagcgcaaa ggtcgcgaaa tacggccagg ccagttcgga caagtggtcc
    12181 ttgtccaggg tcaggccctt ggccgtgacg ttgaacgtcg gcttgcgcgg gttgatcagg
    12241 acgccccaga tgcctttgac gaggtagatc gactggacgt actcataggc ctccgagatc
    12301 catggccagc gtgtatcgcc ataaaggtaa ttctggatcg tcatgtgtgt cagcatgtag
    12361 atcgtgatgt acgcgaaaaa ctcctgagcg ttcgcgacaa aaatctgcat gttgaagaaa
    12421 atatacagga aaggcgacat caggaatatg acgcgcggaa ttggaaaaag ccagtagaac
    12481 atgctggaaa cgtaagcgat gcgctgcttc agggagatgt ttttcttcag aagcgggttc
    12541 ttcagcacga gaatctggat catgccgcgg caccagcgcg aacgctggcc gatgaagctg
    12601 acaaggctct ccggctgaag gcccgcgatc agcggcttgt cgacatagag cgagttccag
    12661 ccgcgtgtat gcaggtccag cgcggtctcg cagtcttccg tgatggagat gccggaaaag
    12721 ccgccgacgc tttccagggc gcggcgtgcg atgagcgccg cggaaccgca gaagaaggct
    12781 ccgttccact tgtcgagacc cttctggatg gtgccgtaga acatctcgtt ctcggacggc
    12841 atccgctcga aggtctgcag gttcttctcg atcgggtccg gattgatgaa gaagtgcggc
    12901 gtctgcacca ggaagagttt gcggtcggtg gcgaagtagc caaccgtctc gcgcaggaac
    12961 tggcgggcag gggcgtggtc ggcgtcgaag acaacgatga tctggccctt cgtctgctga
    13021 agggcattgt tcatgttgcc ggccttcgcg tgctcgttcc gcgcgcgggt gatataggta
    13081 atgccgagtt cggcgcagag cgcctgtagt tccacgcgcc ggtcgcgtgc ttcctgtgcc
    13141 ttctccggat cggcctggtt gcacttctga tccgtgccgc catcgtccag caagtagatg
    13201 ttcagcttgt ccttcggata gtccatctgc gttgcggcgg ccaccgtggc cgccagcaga
    13261 tcggaccctt cgttgtaggt cgggatgtag acgtcgaccg acggcaggcg atcgtcaggg
    13321 agctgcggcg ggggctttcg ctcgatcgga tcggcgatga tgaacagcga gatgaagaac
    13381 atcatcacgc agtacatttc ggccaggtac agggtgaagc ccggcacgaa gttgacccaa
    13441 tcgttcatcg gcgggagcgt cgacgtcgag cgccagtaga cgtagcgcag gatcatggtg
    13501 atgccgagcg acaggaagat cggtcgccag acgccgccca gccggaactt gcggatcagc
    13561 gtcatcacga ggatcacggc caacccgagc gtgaactggg cctgcgtgct gatcggccag
    13621 gtcgtcaggc cgatgatggt ggcggcgagc gcgaaccatc cggcaacgga ggagtacttc
    13681 gtcagcttcg acccgcccga ggacgttgcg actggcgcat cgtagaagcg gcgacgatgc
    13741 tcggcgggct tggcatccgg cgcgacgtcc ggagtcgcga cgacctgaag cggggccgga
    13801 gccggcgatc gaccgcagtc tctacaagac ggtcacgacg
//
LOCUS       DAOMXN010000041        13452 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_2517_length_13452_cov_11.171979, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000041 DAOMXN010000000
VERSION     DAOMXN010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 13452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13452
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2517_length_13452_cov_11.171979"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="PK812_05155"
     CDS             <1..402
                     /locus_tag="PK812_05155"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015822921.1"
                     /note="Acs; catalyzes the conversion of acetate and CoA to
                     acetyl-CoA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase"
                     /protein_id="HRJ68978.1"
                     /translation="DVINVSGHRMGTAEVESALVAHPKVSESAVVGYPHDIKGQGIYA
                     YVTLMGGEKATADLRKELVAWVRKEIGPIATPDLIQFAPGLPKTRSGKIMRRILRKIA
                     EDDFGALGDTSTLADPTVVDDLIANRQNKKA"
     gene            complement(470..1819)
                     /gene="hemN"
                     /locus_tag="PK812_05160"
     CDS             complement(470..1819)
                     /gene="hemN"
                     /locus_tag="PK812_05160"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007198255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HRJ68979.1"
                     /translation="MTPDDIRALAAPVPRYTSYPTAPHFHAGVDATVYGGWLSALPDN
                     AKLSLYFHVPFCDTLCWFCGCHTKITRRYEPVRDYLDALMAETATLGMLVPAPAQVTH
                     IHWGGGSPTMLKPDDIRRLSDATKAAFRLDGSCDFAVEIDPRGLDDAHMDALVAAGLT
                     RVSIGVQDFDIAVQQAINRIQSVEETRRVVDGFRARGVSSLNIDAIYGLPHQTRRRLE
                     NTLREVVRLRPDRIALFGYAHVPWMKKHMSMIEEEALADVVERYHQAELAAALLVGEG
                     YERIGIDHFALPHDSLAIAARAGTLQRNFQGYTVDPADALLGLGASAIGSLPQGYVQN
                     TTATPSYMRAALEGRLAAERGVALSEEDRLRRAVIEQMMCGLEFSTRSLAGADAGLAT
                     ALIGEARAIVNEDTYGFIEPTADGFRVTEAGRPFLRSIAARFDGYLKKQAAKHSLAV"
     gene            complement(1902..2303)
                     /locus_tag="PK812_05165"
     CDS             complement(1902..2303)
                     /locus_tag="PK812_05165"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HRJ68980.1"
                     /translation="MKLLLVAAVALVDTDKRILIAQRPEGKQLAGLWEFPGGKVEPGE
                     RPEHTLIRELKEELGLVVKEPCLAPLTFASHAYETFHLLMPLYICRRWEGTPQPLEGQ
                     KLAWARANDLRNYPMPPADEPLIPMLRDMLL"
     gene            complement(2300..3193)
                     /locus_tag="PK812_05170"
     CDS             complement(2300..3193)
                     /locus_tag="PK812_05170"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRJ68981.1"
                     /translation="MNAAQASPAFVARHLFLCSLGWGSSFLFMKLMAGSVDPVVIAAV
                     RAILAAGVLAIWVGLLGQMPLPRTQELVPWLVLGTFSGWIPNLLVAVAVNHLDSGPAA
                     LIQASSPLMTAVGAHVLFSDERMGRRRLIGVALGLCGVALLIGPRAFEGGGATFAVAC
                     MLAVAACYAATNLYTKTIRDVGAERLALGQQAISALAGLLLALVFSGPQGFAAAAPHL
                     WLLLALGLWSTAMPMAVFMRLIRAAGPTRSAMTGYTVPTVAALLGIIVLGERLTTWQW
                     IGGAVALAGVALVTTSTARPE"
     gene            complement(3190..4440)
                     /gene="argJ"
                     /locus_tag="PK812_05175"
     CDS             complement(3190..4440)
                     /gene="argJ"
                     /locus_tag="PK812_05175"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HRJ68982.1"
                     /translation="MAGKAVPVSPLAPKKQPKLPPLDGVTFATAEAGIRYKGRTDVLL
                     VKLDAGTQVAGVFTRSKCPSAAVDWCRENLGGGKARALVVNSGNANAFTGMKGREAVK
                     LTADIAARELGVKPGEIFLASTGVIGEPLDATKFKGVIGACAKRAEGALWLDAAKAIM
                     TTDTFPKIATRKARIDGHVVTINGMAKGAGMIAPDMATMLSFVFTDAPVAAAALQGLL
                     AKGVKSSFNAVTVDGDTSTSDTLMLFATGTAAARGCPAIAEAGDKRLSGFKRALNALL
                     LDLAIQVAKDGEGARKFVAVTVKGAKSARSARRVAFSIANSPLVKTAVAGEDANWGRV
                     VMAVGKAGEPAERDLLSIWFGPHRVAHKGARDPGYSEEAVSAYMTGERIDIVADLGLG
                     RGRDTVYTCDLTKAYVEINGDYRS"
     gene            complement(4468..5304)
                     /locus_tag="PK812_05180"
     CDS             complement(4468..5304)
                     /locus_tag="PK812_05180"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRJ68983.1"
                     /translation="MLLPSVLRPLLLSSVIVLPLVAWAQPKQPLASVDGTPIIEEDLK
                     TALDDLGQSVERMGEAQRRKYLIDYMIDLRLISRAAEKEKLGEGPDFARKLAYMRERA
                     LMETLMARIGAEATTPEKLKAFYDEAVKGLGPEIEVRARHILVPTEDEAKKAHARVTG
                     GEDFAKVAGELSKDPGSGKEGGDLGFFTKDRMVPEFGEAAFGMKPGEISKPVKSQFGW
                     HVIKLEERREKPVPPLEEVKEELTRYLVQKAQQEMILKLRSAAKIDRPAEPAAPATPP
                     KQ"
     gene            5801..8602
                     /gene="secA"
                     /locus_tag="PK812_05185"
     CDS             5801..8602
                     /gene="secA"
                     /locus_tag="PK812_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HRJ68984.1"
                     /translation="MFGALAKKIFGSSNDRRLKRYRPLVAAVNALEPELEKLTDEQLR
                     ERTAEFKRQVAAGEKTLDDLIVPAFATVREGAKRTLRQRHFDVQLIGGMVLHEGAISE
                     MKTGEGKTLVATLAVYLNALSGKGVHVVTVNDYLARRDAEWMGRIYEFLGLTVGIIVH
                     GLDDEQRKASYACDITYGTNNEYGFDYLRDNMKYDFAQMVQRGHNFAIVDEVDSILVD
                     EARTPLIISGPLDDRSELYNAIDALVPKLTREDYELDEKQRSVALTEAGNERIEEFLS
                     QAGILKEGSLYDAQNATLVHHVNQALRAHTLFQRDKEYIVHNGEIVIIDEFTGRMMPG
                     RRFSEGLHQALEAKEHVQVQPENQTLASITFQNYFRLYDKLAGMTGTAATEADEFQQI
                     YNLEVVEIPTNVPVKRIDDDDEVYRTAEEKYAAIVKLIEECQQNGQPVLVGTTSIEKS
                     EEIAAILLRDGFKQIDLGDANAFAPLYRAAHGGKPEKIFAVLNARFHEQEAHIVAQAG
                     VPGAVTIATNMAGRGTDIQLGGNVDMRIRQELLDKAEGTFEWHQAEEAIRAEVAKLKE
                     KALAAGGLYVIGTERHESRRIDNQLRGRSGRQGDPGHSRFFLSLEDDLMRIFGSERMD
                     SMLQKLGLKHGEAIAHPWVNKALETAQKKVEARNFDIRKNILKYDDVMNDQRKVVFEQ
                     RRDMMALEDMAGQVQEMRHGVIDDLVAKAIPEDAYPEQWDLAVLKEGVANYLNLPDTP
                     VEDWAKEEGIADQEIRERLYRAADESYAARVERNSVEATRYIEKQVILQTLDHLWREH
                     LVTLDHLRHVIGWRGLAQRDPLNEYKAEAFELFDGLVTRLREHVTAQLNRVEVRFEEP
                     PPQPTLPPMTASHINPLTGEDEFAALENVQPLSASGPGRAAVAIDPNDASTWGKVGRN
                     EPCPCGSGKKYKHCHGAYG"
     gene            complement(8628..9374)
                     /locus_tag="PK812_05190"
     CDS             complement(8628..9374)
                     /locus_tag="PK812_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRJ68985.1"
                     /translation="MPLDVVDLKQFYASPLGRFARQAILGGVRARWQTLQGLAVAGVG
                     YPVPYLDELRDQAERSFALMPSAQGVVVWPPGGLAAAALVDPLDLPLRDSVVDRLLVI
                     HALEMSEDPLGLLEELWRILAPGGHLIVVAPNRRGGWSRRDSTPFGHGLPFSRRQLTE
                     LLRQALFTPVHWTEALHLPPFSSTMLLRLAPAVERIGTTLNLPLAGLHLIEATKQIYR
                     PALAGRRARASARLAPVLVPTAGRVGHDAD"
     gene            9497..10258
                     /gene="gloB"
                     /locus_tag="PK812_05195"
     CDS             9497..10258
                     /gene="gloB"
                     /locus_tag="PK812_05195"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018448112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HRJ68986.1"
                     /translation="MRIHQFACRDDNFGVLIQDPSTGACASIDAPEEAAVHRALKTTG
                     WRLTHLLVTHKHPDHIEAVLPLKQDFGCEVIAPTKAKGVVPAVDRYVGEGDRVRVGAL
                     EADVWETPGHCADHVIYHFAAEKVVFAGDVLFGLGCGRVFDGAYDELWRALERLAALP
                     DDTRVYFGHEYTLSNAKFALSVDGDNPALKQAFAEIVEARARGDATSPTTIGREKAAN
                     PFLQATNPAMKARLGMAGQPDAAVFRELRERKNRF"
     gene            10285..10716
                     /locus_tag="PK812_05200"
     CDS             10285..10716
                     /locus_tag="PK812_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRJ68987.1"
                     /translation="MTAEEVIRLLDLRPHPEGGWYRETFRDDRPGSGARAASTLIYFL
                     LRRGEVSHWHRVDATEVWHWHAGAPLALSLSADGRRSESLRVGPDLRTGERPQAVVPA
                     HWWQSAATLGAWTLVGCSVAPGFEFSGFELAPPGWRPDENA"
     gene            10713..11450
                     /locus_tag="PK812_05205"
     CDS             10713..11450
                     /locus_tag="PK812_05205"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRJ68988.1"
                     /translation="MSDLATVQACEERLVNVWPALQTIVMGDWVLRFAQGYSGRANSA
                     SAIRIGADLCEADLDHVMRLYRAEGLAPAIRVTPLSPPDLGARLLATGWRPVVTSIGM
                     VAPAGMWRAHPAVSIQSQAGTAWVQGVSRWQEARKSNPDHLSAILKQLRLPSAFATVA
                     GEGQEAGYGMSVVDRGMAEIGSIMLAPDQRGRGLGRALVEALLCWAVQHGAGRVFLQV
                     EENNVVAIGLYRSLGFQEVYRYTEYRL"
     gene            complement(11458..12327)
                     /locus_tag="PK812_05210"
     CDS             complement(11458..12327)
                     /locus_tag="PK812_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012322026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRJ68989.1"
                     /translation="MSFESQPTPHRRATLIGLAAIALWGLLAVMTAATAGIPPFQLTA
                     MTFAVGGAVRLAFVAARGQLALLRQPPKAWALGLYGLFGYHAVYFAALKLAPPAEASV
                     VAYLWPLLIVLFSALLPGERLQARHLFGAALGFAGVGALAVSRGVTGFSGDALPGYLL
                     ALASAVIWGSYSVLSRLQRDVPTEAVAGYCIGAAALAALCHAAFETTIIPANAWMWAM
                     VILLGLGPVGAAFFFWDHGVKRGDIRFLGVASYATPLISTATLILAGFAEASWGLAIA
                     CGLIVGGAFIARQ"
     gene            complement(12385..13110)
                     /gene="phbB"
                     /locus_tag="PK812_05215"
     CDS             complement(12385..13110)
                     /gene="phbB"
                     /locus_tag="PK812_05215"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HRJ68990.1"
                     /translation="MSRVALVTGGTRGIGAAISLSLKAAGFKVAASYAGNDEAAAKFK
                     AETGIPVFKWDVGDFDACAAGIKQVEAEVGPIDVLVNNAGITRDGFFHKMTKANWDAV
                     INTNLNSLFNMTRNVWEGMMARKFGRVIVISSVNGQKGQAGQTNYSAAKAGDIGFVKA
                     LAQEGVRSGITVNAICPGYIATEMVAAIAPEVLNSKIIPQIPVGRLGKPEEIGRCVVF
                     LASDDSGFITGATISANGGQYMV"
     gene            complement(13211..>13452)
                     /locus_tag="PK812_05220"
     CDS             complement(13211..>13452)
                     /locus_tag="PK812_05220"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018690183.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HRJ68991.1"
                     /translation="VEANEAFAAQALSVNKDLGWNTDIVNVNGGAIAIGHPVGASGAR
                     ILNTLLFEMQRRNAKKALATLCIGGGMGIALCVER"
BASE COUNT         2314 a         4341 c         4629 g         2168 t
ORIGIN      
        1 gacgtgatca acgtctcggg ccaccgcatg ggcacggcgg aagtggaaag cgcgctggtc
       61 gcccatccga aggtctcgga atcggcggtc gtcggctatc cccacgacat caagggccag
      121 ggcatctatg cctatgtcac gctgatgggc ggcgagaagg ccacggccga cctgcgcaag
      181 gaactcgtcg cctgggtgcg caaggagatc ggcccgattg ccacgccgga cctgatccag
      241 ttcgcccccg gcctgccgaa gacccgctcg ggcaagatca tgcgccgcat cctgcgcaag
      301 atcgccgagg acgatttcgg cgcgctcggc gacacctcga cgctggccga tccgaccgtc
      361 gtcgacgatc tgatcgccaa ccggcagaac aagaaggcgt gaggcgcctg tcgtccctca
      421 gggatggacc aatggaaaac cccggccgtg cgccggggtc tttttttgtt cacaccgcca
      481 gcgagtgctt cgccgcctgc ttcttcagat agccgtcgaa gcgcgcggcg atggagcgca
      541 ggaagggccg gccggcctcg gtgacgcgga agccgtcggc ggtcggttcg atgaacccgt
      601 aggtgtcttc gttcactatg gccctggcct ccccgatcag tgcggtcgca aggcccgcgt
      661 ctgctccagc cagtgatcgg gtggaaaact ccagcccgca catcatttgc tcgatcaccg
      721 cgcggcgcag gcgatcctcc tccgacagcg cgacgccgcg ctcggccgca aggcgaccct
      781 ccagcgccgc gcgcatgtag gacggcgtcg cggtcgtgtt ctgcacgtag ccctgcggca
      841 aggaaccgat ggcggaggcg ccgaggccga ggagcgcatc ggcggggtcc acggtgtagc
      901 cctggaaatt gcgctgcagc gtgccggcac gggcggcaat ggcgaggctg tcgtggggca
      961 gggcgaaatg gtcgatgccg atgcgctcat agccctcgcc gaccagcaaa gccgccgcaa
     1021 gctccgcctg atggtagcgc tcgaccacgt cggccagcgc ctcctcctcg atcattgaca
     1081 tgtgcttctt catccagggc acatgggcgt aaccgaacag ggcgatgcgg tccggcctga
     1141 gccgcacgac ttcgcgcaac gtgttttcaa ggcgtctgcg cgtctggtgc ggcaggccgt
     1201 agatggcgtc gatgttgagc gaggaaacgc cgcgtgcgcg gaagccatcc accacgcgcc
     1261 gggtttcctc gacactctgg atgcggttga tcgcctgctg caccgcgatg tcgaaatcct
     1321 gcacgccgat ggagacgcgg gtgaggccgg cggcgacgag cgcgtccatg tgggcgtcat
     1381 cgagcccgcg cggatcgatc tcgaccgcga agtcgcaaga gccgtcaagc cggaaggccg
     1441 ctttggtggc gtcggacagg cggcggatgt cgtccggctt cagcatggtg ggcgagccgc
     1501 cgccccagtg gatgtgggtg acttgcgcgg gggccggcac gagcatccca agcgtcgcgg
     1561 tctcggccat cagcgcgtcg agatagtcgc gcaccggctc gtagcggcgg gtgatcttgg
     1621 tgtggcagcc gcagaaccag cacaacgtgt cgcagaacgg tacgtggaaa tagagcgaca
     1681 gcttggcgtt atcgggcagg gcgctgagcc agccgccata gacagtggcg tcgacgccgg
     1741 catggaaatg cggcgcggtc gggtagctgg tgtagcgcgg cacgggcgcg gccagcgctc
     1801 ggatgtcgtc aggcgtcaag gtcacggcgc aatccccctc ggatgcgagg tctttgtgac
     1861 gccttgcgcg gcttcaggcc ttgagatgcg tcaatccgct ttcagagcag catgtcccgc
     1921 agcatgggga tcaacggctc gtccgccggc ggcatcgggt agttccgcag gtcgttggcg
     1981 cgagcccagg ccagcttctg gccttccagc ggctgcggcg tgccctccca gcgccggcag
     2041 atgtagagcg gcatcagcag atgaaacgtc tcgtaggcgt ggctggcgaa ggtgagcggg
     2101 gcaaggcagg gctccttcac gacaaggccc agttcctcct tcagctcacg gatcagggta
     2161 tgttccggtc gctcgcccgg ctcgaccttg ccgccgggaa actcccagag gccggccagt
     2221 tgctttcctt ccgggcgctg cgcaatgagg atgcgcttgt ccgtgtcgac cagcgccacg
     2281 gcggcgacga gcaagagttt cattcgggcc tcgctgtcga ggtggtgacc agggcgacgc
     2341 cggccagcgc cacggcgccg ccgatccact gccatgtggt gagccgctcg ccgagaacga
     2401 tgatgccgag cagggcggcg acggtgggca cggtgtagcc ggtcatggcg gagcgtgtcg
     2461 gtcctgcggc gcggatcagc cgcatgaaca cggccatcgg catggcggtg gaccagaggc
     2521 cgagggcgag cagcagccac agatgcggcg cggcggcggc gaagccctgg ggaccggaaa
     2581 agaccagcgc gagcagcagt ccagccaggg cggaaatcgc ctgctggccg agcgcgaggc
     2641 gttcggctcc cacatcgcgg atcgtcttgg tgtagaggtt ggtggcggca tagcaggcag
     2701 cgacggccag catgcaggcc accgcgaatg tggctcctcc cccttcgaag gcgcgcgggc
     2761 cgatcagcag tgcgacacca cagagtccga gggcgacgcc gatcagccgt cgccggccca
     2821 tccgttcgtc ggagaacagg acatgcgccc ctaccgccgt catgagcgga ctggaggcct
     2881 ggatcagcgc tgccgggccg gagtcgaggt ggttcaccgc cacggccaca agcaggttgg
     2941 gaatccagcc ggagaaggtg ccgaggacca accagggaac gagttcctgc gttcttggca
     3001 gcggcatctg gccgagcagc ccgacccaga ttgccagcac gccagcggcg aggatcgcgc
     3061 gcacggcggc aatgacgacc gggtcgaccg atccggccat cagtttcatg aacaggaagc
     3121 tggatcccca gccgagcgaa cacaggaaca gatgccgggc gacgaacgcc ggtgacgcct
     3181 gcgccgcgtt cacgaacggt agtcgccgtt gatctcgacg taggccttgg tgagatcgca
     3241 ggtatagacc gtgtcccggc cgcgccccag tcccaggtcg gcgacgatgt cgatgcgctc
     3301 gcccgtcatg taggcggaca ccgcctcctc gctgtagccg ggatcgcgcg cgcccttatg
     3361 cgccacgcgg tgcgggccga accagatcga caggaggtcg cgctcggccg gctcgccggc
     3421 cttgcccacc gccatgacga cgcggcccca gttggcgtcc tcgccggcga cggcggtctt
     3481 caccaggggc gaattggcga tggaaaaggc gacgcgccgg gcggaccgcg cggacttcgc
     3541 gcccttcacc gtgaccgcca cgaacttgcg cgcgccctcg ccgtccttcg ccacctggat
     3601 ggcgaggtca agcagaagcg cgttcaacgc gcgcttgaag cccgaaaggc gcttgtcgcc
     3661 ggcttcggcg atggccgggc agccgcgagc ggcggcggtt ccggtggcga acagcatgag
     3721 cgtgtcggag gtggaggtgt cgccgtcgac cgtcacggcg ttgaaggagg atttgacacc
     3781 cttcgccagc agcccttgca gcgcggcagc ggccacgggc gcatcggtga agacgaagga
     3841 aagcatggtg gccatgtccg gggcgatcat gccagcgccc ttggccatgc cgttgatcgt
     3901 caccacatgc ccgtcgatcc tggccttgcg cgtggcgatc ttcgggaagg tgtcggtggt
     3961 catgatggct ttcgccgcat cgagccagag ggcgccttcc gcccgcttgg cgcacgcgcc
     4021 gatcacgccc ttgaacttgg tggcgtccag cggttcgccg atgacgccgg tcgaggcgag
     4081 gaagatctct cctggcttca cgccgagttc cctggccgcg atgtcggccg tgagcttgac
     4141 cgcctcgcga cccttcatgc cggtgaaggc gttggcattg ccggaattga cgaccagcgc
     4201 ccgcgccttg ccgccaccca ggttctcgcg gcaccagtcc acggcggcgg acggacactt
     4261 ggagcgggtg aacacgccgg cgacctgcgt tccggcgtca agcttcacca ggagcacatc
     4321 ggtgcgcccc ttgtagcgga tgcccgcctc cgccgtggcg aaggtcacgc cgtcgagcgg
     4381 cggcagtttg ggctgcttct tcggtgcaag gggtgaaacg gggacggctt taccggccat
     4441 gatgcggatc ctgtcgggtt cttcaaatca ctgcttgggc ggtgtcgcgg gagcggccgg
     4501 ctccgccgga cggtcgatct tggctgctga gcgcagtttc aagatcattt cctgctgtgc
     4561 cttctgaacg agatagcggg tcaattcctc cttgacctcc tcaagcggcg gtaccggctt
     4621 ctcgcggcgc tcctccagct tgatgacatg ccagccgaac tgcgacttga ccggcttcga
     4681 gatttcgccg ggcttcattc cgaaggcggc ctcgccgaat tccggcacca tgcgatcctt
     4741 ggtaaagaac ccaaggtctc cgccctcctt gcccgaaccc gggtccttgg acagctcacc
     4801 cgcgaccttg gcgaaatcct ccccgcccgt gacgcgcgcg tgggccttct tggcctcgtc
     4861 ctcggtcggc acaaggatgt gacgggctcg cacctcgatt tccgggccca ggcccttgac
     4921 cgcctcatcg tagaaggcct tgagcttttc cggcgtggtc gcctcggcgc cgatgcgcgc
     4981 catcagcgtc tccatcaggg cacgttcgcg catgtaagcg agcttgcgcg cgaaatccgg
     5041 gccctcgccc agcttttcct tctccgccgc gcgtgagatc aagcgcagat cgatcatata
     5101 gtcgatcagg tatttgcgcc gctgcgcctc gcccatgcgc tcgacactct gtccgagatc
     5161 atcgagcgcc gtcttgaggt cctcctcgat gatcggtgtc ccgtcgacgc tggccaaggg
     5221 ctgtttcggc tgggcccagg cgaccagcgg caggacgatg acgctcgaca gcaggagcgg
     5281 gcgcaggaca gaggggagca gcatcgggtt ctccggggaa atcagcgtat ctgacgggtt
     5341 cgcatgaagc gcggcgatct tcaacccttg atcgaggcag tgtgatcggc gctggtcccg
     5401 gccgcgctcg gtcgccgcta tttcggtcga aaccgtctct attccagtgc gcgcgtgccg
     5461 ttgttcgctc ggggctctgg acggggtata ggcttcccga gcactcaagt ctgccgacac
     5521 cgggcgagat tctcgctggg gtgtacgggg ggcgcatagg tggacaggcc gatgacgaag
     5581 cctggccgga acgggcagca cgaagcctct ggtgatggac tttcgtgctg ttccgcacct
     5641 tccgagcggt ggcgtgggcc tgattcattg acaagacggg ctgtcgctcc ttatctccgc
     5701 gtcacgctgc ggaggccgtt ccgcggcgag aatcggtgtt tgtcgcatcc gcagcgggag
     5761 acaccccgac gggccagcgg cccagcgaag gatcgcattg atgttcggcg cgctcgccaa
     5821 gaaaatattc ggttcatcca acgaccgccg cctgaaacgg taccgaccgc ttgttgccgc
     5881 cgtcaacgcc cttgagcccg agctcgagaa gctgaccgac gagcaactgc gcgagcgcac
     5941 ggccgagttc aagcgtcagg tcgcggccgg cgagaagacc ctggatgatc tgatcgtgcc
     6001 ggccttcgcg acggtgcgcg aaggcgccaa gcgcacgctc aggcaaaggc acttcgacgt
     6061 ccagctcatc ggcggcatgg tgctgcacga gggtgccatc tcggaaatga agacgggcga
     6121 aggcaagacg ctggtcgcca cgcttgcagt ctatctcaac gccctttccg gcaagggcgt
     6181 gcatgtcgtg acggtgaacg actacctcgc ccggcgtgac gccgagtgga tggggcgcat
     6241 ctacgagttt ctcggcctga ccgtcggcat catcgtgcat ggcctcgacg atgagcaacg
     6301 caaggcgtcc tatgcctgcg acattacgta cggcaccaac aacgaatacg gcttcgacta
     6361 cctgcgcgac aacatgaagt atgacttcgc gcagatggtg cagcgcgggc acaatttcgc
     6421 gatcgtcgac gaggtggact cgatcctggt cgacgaggcg cgcacgccgc tgatcatttc
     6481 cggcccgctc gacgatcgct cggaactcta caacgcgatt gacgcgctcg tgccgaaact
     6541 gacgcgcgag gactacgaac tcgacgagaa gcagcgctcg gtcgcgctga ccgaagccgg
     6601 caacgagcgg atcgaggagt tcctcagtca ggccggcatt ctcaaggaag gctcgctcta
     6661 cgacgcccag aacgcgactc tggtgcatca cgtcaaccag gccttgcgcg cgcacacgct
     6721 gttccagcgc gacaaggagt acattgtcca caacggcgaa atcgtcatca tcgacgagtt
     6781 taccggacgc atgatgccgg gccggcgctt ctcggaaggc ctgcaccagg cgctggaagc
     6841 caaggagcat gtgcaggtcc agccggagaa ccagacgctc gcctccatca ccttccagaa
     6901 ctatttccgc ctctacgaca agctcgccgg catgaccggc acggcggcga ccgaagcgga
     6961 cgagttccag cagatctaca acctcgaggt cgtcgagatc ccgaccaatg tcccggtcaa
     7021 gcgcatcgac gacgacgacg aggtctaccg gacggcggag gaaaagtacg cggccatcgt
     7081 caagctgatc gaggaatgcc agcagaacgg tcagcccgtg ctggtcggca caacctccat
     7141 cgagaagtcg gaggagatcg cggcgatcct gctgagggac gggttcaagc aaatcgacct
     7201 cggcgacgcc aacgccttcg ctccgctcta ccgggcggcg cacggcggca agccggaaaa
     7261 aatcttcgcc gtgctgaacg cgcgcttcca cgaacaggaa gcccatatcg tggcgcaggc
     7321 cggcgtgccc ggcgccgtca ccatcgccac caacatggcc ggtcgcggca cggacatcca
     7381 gcttggcggc aacgtcgaca tgcgcatccg tcaggagctg ctggacaagg cggagggcac
     7441 cttcgagtgg catcaggccg aggaggcgat ccgcgccgaa gtcgccaagc tgaaggagaa
     7501 ggcgctcgcc gccgggggcc tctacgtcat cggcacggag cggcacgaga gccggcgcat
     7561 cgacaaccag ttgcggggcc gctcaggccg ccagggcgac ccgggccatt cccggttctt
     7621 cctgtcgctg gaagacgatc tgatgcgcat cttcggctcg gagcgcatgg attccatgct
     7681 gcagaagctc ggcctcaagc acggcgaggc catcgcccac ccctgggtca acaaggcgct
     7741 cgaaacggcg cagaagaagg tcgaggcgcg caacttcgac atccgcaaga acatcctgaa
     7801 atacgacgac gtgatgaacg accagcgcaa ggtcgtgttc gagcagcgcc gcgacatgat
     7861 ggcgctggag gacatggccg ggcaggtgca ggagatgcgt cacggcgtca tcgacgatct
     7921 ggtggccaag gccattcccg aggacgccta tcccgaacaa tgggatctcg cggtgcttaa
     7981 ggaaggcgtc gccaactacc tgaacctgcc cgatactccg gtcgaggact gggccaagga
     8041 agaaggcatc gccgatcagg aaatccgcga acgcctctac agggcggcgg atgagtctta
     8101 tgcggcccgc gtggagcgca attcggtcga ggccacgcgt tacatcgaga agcaggtgat
     8161 cctgcagacg ctcgatcatc tgtggcgcga gcatctggtg acgctggatc atctgaggca
     8221 tgtcatcggc tggcgcggcc tggcccagcg cgatcccctg aacgaataca aggcggaagc
     8281 gttcgaactg ttcgatgggc tggtcacgcg cctgcgcgag cacgtgacgg cgcagctcaa
     8341 ccgggtcgag gtgcgcttcg aggaaccgcc gccgcagccg acgctgccgc cgatgacggc
     8401 atcgcacatc aaccccctga ccggtgagga cgagttcgcc gcgctggaaa acgttcagcc
     8461 cctatcagcc tcgggaccag gccgcgcggc cgttgcgatc gatccgaacg atgcctcgac
     8521 ctggggcaag gtcgggcgaa acgagccctg cccctgcggt tcgggcaaga agtacaagca
     8581 ctgccacggc gcttacggct gaggtcgatc tctcccggcg tttcggatca gtccgcgtcg
     8641 tggccgacgc ggccggcggt cggcaccagc actggcgcca gccgcgccga agcccgggcg
     8701 cgtcgtccgg cgagggccgg gcggtagatt tgcttggtcg cctcgatcag atgcaatccg
     8761 gccagcggaa ggttgagcgt tgtgccgatc cgttccaccg ccggcgccag acgcagcagc
     8821 atcgtgctcg agaacggggg caggtgcagc gcttccgtcc agtgcaccgg cgtgaacaat
     8881 gcctgtcgca gcagttcggt aagctgccgg cggctgaacg gcagcccgtg cccgaacggc
     8941 gtcgaatcgc gacgcgacca gccgccgcgc cggttcgggg cgacgacgat caggtgaccg
     9001 ccgggggcga ggatgcgcca gagttcttcc agcaggccga gcgggtcctc gctcatctcc
     9061 agcgcgtgga tgacgagcag acgatcgacg acggaatcgc gcaggggcag gtccagcggg
     9121 tccaccagcg ccgccgccgc cagcccgccg gggggccaga ccaccacgcc ctgcgcgctc
     9181 ggcatcagcg cgaatgaccg ttccgcctga tcgcgcagtt cgtcgaggta gggaacgggg
     9241 tagccgacgc ccgccaccgc aagcccttgc agggtctgcc agcgcgcgcg gaccccgcca
     9301 aggatggcct ggcgggcgaa gcggccgagg ggcgaggcat aaaattgttt gaggtcgacg
     9361 acgtccaggg gcatgatgcc aatattggcg ctggctccgg ggaaagaaag ggcggcgtca
     9421 gtttggttca tacaggaact gttcatttat gaacctttat tgccgctctg aaccaacgac
     9481 actgtgggga tagaccgtgc gcattcacca gtttgcgtgc cgggacgaca atttcggggt
     9541 cctgatccag gatccgtcga ccggcgcctg tgcttccatc gacgctcctg aggaggcggc
     9601 cgtgcaccgc gccctcaaga ccacgggctg gcgcctgaca cacctgcttg tcacgcacaa
     9661 gcaccccgac catatcgagg ccgtcctgcc gctgaagcag gatttcggct gcgaggtgat
     9721 cgcgccgacc aaggcgaagg gtgtcgtgcc ggccgtggac cggtacgttg gcgagggcga
     9781 tcgtgtgcgg gttggcgcgc tcgaagctga cgtttgggaa acacccggcc attgcgccga
     9841 tcatgtgatc taccatttcg ccgccgagaa agtggtcttc gccggggatg tgctgttcgg
     9901 cttggggtgt gggcgggtat tcgacggcgc ctatgacgag ctttggcgcg cactcgagcg
     9961 tcttgccgcc ctgcccgacg acacgcgcgt ctacttcggg cacgaataca cactttcgaa
    10021 cgccaagttc gcgctcagcg tcgatggcga caatccggcg ctgaagcagg ccttcgccga
    10081 gatcgtcgag gcgcgtgcgc ggggcgatgc gacctcgccg acgaccatcg gtcgcgagaa
    10141 ggcggccaat cccttcctgc aggcgacgaa tccggccatg aaggcgcgac tcgggatggc
    10201 ggggcagccg gatgcggcgg tcttccgcga gttgcgtgag cgcaagaacc ggttctgatg
    10261 gcggcggcaa cggttgcgga tgcgatgacg gccgaggagg tgatccgcct cctcgacctg
    10321 aggccgcatc cggaaggggg ctggtatcgc gagacatttc gtgacgaccg ccccgggtcg
    10381 ggcgcccgcg ccgcttcgac gctgatctac ttcctgctgc gccggggaga agtgtcgcac
    10441 tggcaccggg tggacgcgac cgaggtctgg cattggcatg ccggcgcgcc gctggcgctg
    10501 tcgctcagcg cggacggccg gcgttcggag agcctgcggg ttggtccgga tctgcgtact
    10561 ggcgagcggc cgcaggccgt tgttccggcg cactggtggc aatccgccgc cacgcttggg
    10621 gcatggacgc tggtcggctg ttcggtcgcg ccgggcttcg agttctccgg cttcgaactg
    10681 gcgccgcccg ggtggcgccc ggacgagaat gcatgagcga cctcgcgacg gtgcaggcct
    10741 gcgaggagcg gctggtcaat gtctggccgg ccctacagac gatcgtgatg ggcgactggg
    10801 tgctgcgctt cgcccagggc tattcggggc gggccaattc cgcgagtgct atccggattg
    10861 gcgcagacct gtgcgaggcc gatctcgatc atgtcatgcg gctgtatcgc gccgaagggc
    10921 tggcgccggc catccgtgtc acgccgctgt cgccgcctga cctcggtgca aggctgctgg
    10981 cgacaggctg gcgaccggtc gtcacctcca tcggcatggt cgcgccggcc ggcatgtggc
    11041 gagcccatcc ggccgtgtcg atccagtcgc aggcagggac ggcctgggtt cagggcgtca
    11101 gccgctggca ggaggcgcgc aagagcaatc ccgatcacct ctcggcgatc ctgaagcagc
    11161 tgcgcctgcc atcggccttc gccaccgtcg cgggggaagg acaggaggcg gggtacggca
    11221 tgagcgtcgt cgaccgcggc atggcggaga tcggctccat catgctggcg ccggatcagc
    11281 ggggccgcgg actgggccgg gccctggtgg aagcgctgct gtgctgggcc gtccagcatg
    11341 gcgccggtcg ggtgtttctc caggtggagg aaaacaacgt ggtggcgatc ggcctgtacc
    11401 ggtcgctcgg gttccaggag gtctaccggt acaccgagta tcggctctga cgccaggtca
    11461 ctgccgggcg atgaacgcgc cgccgacaat cagcccgcag gcgatggcca gcccccagga
    11521 ggcttcggcg aagccggcga ggatgagcgt cgccgtcgag atcagcggcg ttgcgtagga
    11581 ggcgacgccg aggaagcgga tgtcgccgcg cttgacccca tggtcccaga agaagaacgc
    11641 cgcgcccacc ggcccgagcc cgagcaggat caccatcgcc cacatccagg cattggcggg
    11701 gatgatggtg gtttcaaaag cggcgtgaca taaagccgcc agcgcggccg caccgatgca
    11761 gtagcccgcg acagcctcgg tcggcacgtc gcgttgcagg cgcgagagca cggaatagct
    11821 gccccagatc acggccgagg ccagcgccag caggtatccg ggcagcgcat cgccggaaaa
    11881 tccggtgacg ccgcggctga cggccagcgc cccgacgccc gcaaatccca gcgccgcgcc
    11941 gaacaggtgg cgggcctgca atcgttcgcc cggcagcagg gcggagaaca gcacgatcag
    12001 caggggccag agataggcga cgacgctggc ttccgccggc ggggccagct tgagcgcggc
    12061 gaagtagacc gcgtgatagc cgaacaaccc gtagaggccg agcgcccagg cctttggtgg
    12121 ctggcgcagc agggccagct gcccccgcgc cgcgacgaag gccagcctga cggcgccgcc
    12181 gacggcaaag gtcatggcgg tgagctggaa gggcgggatt ccggccgtgg cagcggtcat
    12241 caccgcgagc agaccccaca gcgcgatggc ggcaaggccg atcagcgtgg cgcggcggtg
    12301 gggcgtgggt tggctctcaa acgacatcgg ccgcgcgtaa cacgcgcggc cgatgcctgt
    12361 cgatggagcg gaagccgccg gcgatcagac catgtactgg ccgccgttgg cggagatggt
    12421 cgctccggtg atgaagcccg aatcgtcgga ggcgaggaac acgacgcagc ggccgatctc
    12481 ctccggcttg ccgaggcggc cgaccggaat ctgcgggatg atcttgctgt tgagcacttc
    12541 cggcgcgatg gccgcgacca tttccgtggc gatatagccc gggcagatgg cgttgacggt
    12601 gatgccggag cgcacgcctt cctgcgccag cgccttcacg aagccgatgt cgccggcctt
    12661 ggccgcgctg tagttggtct ggccggcctg gcctttctgg ccattgacgg aggagatcac
    12721 gatcacgcgg ccgaacttgc gcgccatcat gccttcccag acgttccggg tcatgttgaa
    12781 cagcgagttc aggttggtgt tgatcaccgc gtcccagttc gccttggtca tcttgtggaa
    12841 gaagccgtcg cgcgtgatgc cggcgttgtt caccagcacg tcgatcgggc cgacctcggc
    12901 ctccacctgc ttgatgccgg cggcgcaggc gtcgaagtcg ccgacgtccc acttgaacac
    12961 cgggatgccg gtctcggcct tgaacttggc ggcggcctcg tcattgccgg cgtagctcgc
    13021 cgcgaccttg aagccggcgg ccttcagcga cagcgagatg gcggcgccga tgccgcgtgt
    13081 tccgccggtt acgagtgcaa ctctgctcat gatgcgtttc ctcttgctct ggatgtttcc
    13141 tgcaaccctg cggtggcctg tcagggatcg ggacgccgcc aggaagcgac gtccccgtgg
    13201 tgcgcccgac tcagcgctcg acgcagagcg cgatgcccat gccgccgccg atgcacagcg
    13261 tggcgagggc cttcttggcg ttgcggcgct gcatttcgaa cagcagcgtg ttgaggatgc
    13321 gcgcgccgga cgcgccgacc gggtgaccga tggcgatggc gccgccgttc acgttgacga
    13381 tgtcggtgtt ccagccaagg tccttgttca cggacagggc ctgcgccgcg aaagcctcat
    13441 tggcctcgac ga
//
LOCUS       DAOMXN010000042        13362 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_2534_length_13362_cov_11.789058, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000042 DAOMXN010000000
VERSION     DAOMXN010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 13362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13362
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2534_length_13362_cov_11.789058"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..5242
                     /locus_tag="PK812_05225"
     CDS             14..5242
                     /locus_tag="PK812_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012453305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HRJ68992.1"
                     /translation="MAVTVLSAPVLAQQKSFIREDLASNGIRLEERLKKDGAALASGR
                     PAAQLRQEGMAVLGRDARRALALFGAAIAAEPKSAASWLAYARAAMAIDPGTNYGERY
                     TLRENATTAAYLSYVLANARAEEALALEWLGRAFARADAWRPMLDAYRNSLERADNAP
                     LRALYQEMREKHGFRFDENAPYKVDSDAASPRVCFQFTEALQRGRVDFAPYVAVTGAA
                     SFAVSAEDRQLCVDGLKHGERYTMIVRQGLPSSVGEALLKTADYEIYVRDRSPQVRFT
                     GKNYVLPRVGQEGIPVVSVNAQKVNVDIARVGDRSLLPTVRSEEFLNQISSGQAKKFA
                     NETGFKIWSGTLDIKSEQNKDITTAFPVLEAVGKLEPGVYVMTAWPESLNARSSGSGN
                     GDDDDYSDRSTQWFVVSDLGLTAVSAADGVHVFSRSLASSKALGGVEMRLVAKNNEIL
                     ATQKTDSRGYVKFDPGLSRGMGGLEPGMVVATDAGGDYGFLDLQQAAFDLTDRGVKGR
                     AAPGPLDAFVFAERGVYRSGETVQLTTLLRDAKGVAAGGLPLTLVAQRPDGVEYKRVR
                     VEDQGLGGRAYSLALLSGAPRGTWRVKAYADPKGKPIGETSFLVEDYVPERLDVDLKP
                     KEPLLRSGQPTEVDVSVNYLYGAPGADLEVGGEVTVRLAGATTVPGLKDYVTGLDDET
                     FESTTSELEERATTDARGRATVSVSIPEATAPRPLEARIVLRVGESGGRAVERSVVLP
                     IRPNGPVLAVKKLFSDDSLSEGSIATFDVVAATAEGEKLPRNGVQWTLSRVERRYQWY
                     NSDGRWSFEPVKSTRRVADGRIDVTAAAPGRISAPVGWGTYRLEVRADGSNLAPVATT
                     FTVGWSGESTATTPDLLESSLDKPSYKPGDRLQVKLNPRFAGTATVMIVGDGVHDVQL
                     VDVPKEGSSITIPVKAEWGAGAYAVAFAHRPLDQAAQRMPGRALGVAWFSIDQPAQKL
                     AVELGTPEKIRPRGMLSIPVKLTGAAPGDEAYVTVAAVDVGILNLTGYELPDASGHYF
                     GQRQLGADIRDLYGFLIDGMQGTRGAIRSGGDGGKPSLDGAPPQQVPMARYSGVVKVG
                     TDGTAQVEFDIPAFNGTARVMAVAWTRSGVGQTKKDVIIRDPVVIAGTLPRFLNYGDE
                     SRFFLQIDNIEGGAGDYTLDLDLRGPVIVAADAARRTVRLNPGGKTSLSIPVKAGGLG
                     TATFDLRMTGPGVDVTHSFNVTVLPGTPEVFQRSVRMLNPQQNVTLSSDLLAEYLPKT
                     GYLSVAVSPFGGMDVPALLRALDRYPYGCSEQIVSRAMPLLYVNRLARAEALSLDPNL
                     DERIKDAIERVLSRQDSSGAFGLWAAGGADDHWLDSYVMDFLTRARERGFVVAQKSFD
                     LALDRLRNHVANTSEVRDGGRDLAYAIYVLARNGRPVMNDLRYLVDTKLSDFTTPLAR
                     AQLAAALALLGDRGRAGSVFSNAAEELSKDKMSLSSRSDYGSKLRDGAGILTLAAEAN
                     LSRDVLVKTAAVVFDTRQATRYTSTQENAWMVLAAQALVADAQAMALTVNGEAHKGAL
                     YRTYSAERLEARPLTIENAGQAPAHLVVSASGNPLVQEPALAQGYRVERDLHKLDGTK
                     VDPTKIKQNDRLVVVLKVTELEARFARLLLVDMLPAGLEIDNPELVEGGSLEGFSWLK
                     REAEPVNTEYRDDRFVAAFHRSGEKTAEFSVAYLVRAVAPGAYVHPPAVIEDMYRPER
                     FGRTAYGRVEVAPARN"
     gene            5244..7349
                     /gene="pbpC"
                     /locus_tag="PK812_05230"
     CDS             5244..7349
                     /gene="pbpC"
                     /locus_tag="PK812_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HRJ68993.1"
                     /translation="MVRDETSRLRRYGRRLAWVGVAAVAIVALATAGYRTALNRLGPL
                     DLAAVGDGSPVVLDRAGKLLRPFTTADGRWKLPVAAAEIDPLFFRLLKTYEDRRFDRH
                     GGVDVLAMARASLQAIRSGRVVSGGSTLTMQVARLLEPRDERTVPAKARQVLRAWQLE
                     ESASKQRILDYYVVLAPYGGNIEGLRAASIAYFGKEPRRLSLAEAALLVALPQSPEAR
                     RPDRFAETARKARNRVIDIAVARGTVSASDGESAKGEPVPHQRKAFPMLAPHAAEAAV
                     RARPGERVHRLSIDARLQASLEPIARERVEALGAKVAAAILVVDNATGEVRAHVGGSE
                     YLSAQRAGGVDLMQAVRSPGSALKPFIYGLAFDAGIAHPETQLEDRPSRFATYAPENF
                     DMTFQGTVTARKALQQSLNVPAVELLAEVGPSTFITRLRSAGADIVLPKDAVASLAAA
                     LGGLGIRPVDMAKLYAGLARGGMTQPLTWRLGEGARPERERRLLDAVSSWYLADTLRG
                     TPPPTNALSNRISFKTGTSYGYRDAWAAGFDRRTTIVVWVGRPDGAPVPGLTGRSAAA
                     PILFDAFSRLGGENEVIPAPRNVLMAATTQLPPPLQRLRKDAPKTLAAAAPSLKIAFP
                     PDGARIDLGFAAGRERSDLVLKANGGQLPLIWMVNGRPVSQGELRRQSTWQPDGAGFA
                     RVSVMDAAGSVDSVTVRVE"
     gene            7451..8203
                     /locus_tag="PK812_05235"
     CDS             7451..8203
                     /locus_tag="PK812_05235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRJ68994.1"
                     /translation="MALSKVAVITGAGSGVGKAGALALAGEGFAIVLAGRRKEQIDAV
                     AAKIASKGGQALAVVTDVTDPQSVERLFAEAKRAFGRVDLLFNNAGISAGGPIEDLSV
                     EDWKRVVDTNLTGPFLCTREAFRIMKAQTPRGGRIINNGSISAYAPRPNSAPYTATKH
                     AITGLTRSTALDGRKHDIACGQIDIGNAATDMAMRMASGVPQANGEIAKEPLMDPQCV
                     ADALVAMARMPPDANVLFMTVMATKMPFVGRG"
     gene            complement(8212..9264)
                     /locus_tag="PK812_05240"
     CDS             complement(8212..9264)
                     /locus_tag="PK812_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ68995.1"
                     /translation="MIEAIMYLAIGALLAGLLAIALLPALWRRAERLSRQRLMATLPV
                     TPNEMAAEKDQIRAAAAVEVRRLEVRLDATDALVQQARKEIGDHVATIQQREATIGER
                     NASIAALQADLVARHEDIAALNQRYHALRNERDNLVTNLDATRLFNQELDQESLTQRQ
                     LAEQRLARLAELERQLATLTEKTSEQQAVIAALRDDLRARTSDLRATMQGLRETTAQK
                     SLAERRLAAAEALGEERGAQIAQLQAERLRQIDEIGQITRERNSERVEREARDNEIAR
                     LNTRIAETESYAARAQLAHQEVVQDMTATIEALRQQKRQGDAEIAAVRRDKARAETQI
                     TQRRARSPRQRKTPAE"
     gene            complement(9355..9924)
                     /locus_tag="PK812_05245"
     CDS             complement(9355..9924)
                     /locus_tag="PK812_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1134 domain-containing protein"
                     /protein_id="HRJ68996.1"
                     /translation="MIAQAARALLIIAFLAAGGAQAQQQQKPVNTYESRELVDSGHKF
                     FGTVSRGLALTVEEAVRRWGEPNGYILGQEGSGAFIGGLRYGEGMLYTRNAGDTRVFW
                     QGPSLGFDFGGEGARTMMLVYNLPSVANLFRRYAGIDGSAYFIGGFGMTALGSEGVII
                     VPIRSGVGARLGVNVGYLKFTDQPTWNPF"
     gene            10116..10784
                     /locus_tag="PK812_05250"
     CDS             10116..10784
                     /locus_tag="PK812_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphotransferase family protein"
                     /protein_id="HRJ68997.1"
                     /translation="MSDVASPPIALPIALDSLDLAALLCSRVCHDVISPVGAIVNGLE
                     VLDDDSDQETQDFALALIRKSAQQASARLQFARLAFGAAGSAGASIDLGDAEQVTKSF
                     LQDDKVQIVWQAPRLLLAKNKVKLLLNLLVIAAASIVRGGTITVSLSDSDGQTAISIT
                     AQGPNSRMPPHAVALIAGESATGGIDAHGIQAYYTGLVARASGMAVTLEHAGDTLTIS
                     ARST"
     gene            10919..>13362
                     /locus_tag="PK812_05255"
     CDS             10919..>13362
                     /locus_tag="PK812_05255"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HRJ68998.1"
                     /translation="MDDLLSDFAVETLDSIDSLDAEIVRLERDPSDAAAIERIFRVVH
                     TIKGTCGFINLRRLERISHALENRISIFRDGATPDRAAVNAVFATLDRIRVIARAIET
                     VGVEPAGDDEDLLSALVQEGIGATGRPSQGGHRSFETGSLVYQVLERPLREGEVTLDD
                     LERAFRQAPGPEGATGTPGGPAAGANHSASTAKARVRVAVERLDHLIDVVSELVLIRN
                     QLLDLSRKDGSGAYKLPLQRLSNITGELQDGVMQTRMQAISTAWAGLPRTVRDLALSL
                     GKDIVLETSGDDTEIDRQLLEIIKDSLVHLVRNAADHGIELPAERIAADKAPTGIIRL
                     SAGRERGQIVVRIEDDGRGLDPDALRQRAVARGLLDEAESRALGDSEAALLIFRPGFS
                     TASAVTSVSGRGVGLDAVRDSVEQAGGTIELLNRPGHGLAVILKLPLTLAIANVLIVE
                     SAGQRFALPQLAVTELVRTQADSDVRVEMIGTTLTLRLRDGLLPLIRLDQALGREAQD
                     APAGVVAICQAGHVRFGLALGIILQTEEIVVKPLPGALRSIPYYSGTTILGDGSAVLI
                     LEPAGFARLVEGAGASAPPVPESRPETERQAAKTAVLVFRGHDGREKAVPLSLVKRLE
                     DFSPQDVERLGGRQVVQYRGGVMHLLGEPGLPEQAEREDRMPVIVFSCGRETVGVVVQ
                     EIVDIVEDRFELDLAHASPGWLGTAIIGGRLMEVLDVAALFAAGAAVVPEIPGHGPPR
                     VLLVEPSAFFQAMLAPLLRAVGLDVTIASNADEALALAEANRFAVAIVDFDGAVSRAG
                     ELCMALRPDLKAPALV"
BASE COUNT         2131 a         4316 c         4558 g         2357 t
ORIGIN      
        1 cctcggcctc ttgatggccg tgaccgttct ttccgcaccg gttctcgcgc agcaaaagtc
       61 gttcatccga gaggacctcg cctcgaacgg catacggctt gaggagcgcc tgaagaagga
      121 tggggcagcc ctcgcctcgg gtcgcccggc cgcgcagctg cgtcaggaag gcatggccgt
      181 cctcggccgg gacgcccgcc gcgcgctggc cctgttcggc gccgcgatcg ccgcggaacc
      241 gaaatcggcc gcgtcctggc tggcctatgc gcgggctgcg atggcgatcg atcccggcac
      301 caactacggc gagcgctata ccctgcgcga gaacgccacc acggcggcct atctctccta
      361 tgtcctggcg aatgcgaggg cggaggaggc gctggcgctg gagtggcttg gccgcgcctt
      421 cgcccgtgcg gatgcctggc gcccgatgct cgacgcctat cgcaacagcc tggagcgggc
      481 agacaacgcc ccccttcgcg cgctctatca ggagatgcgc gagaagcatg ggttccgctt
      541 cgacgagaat gcgccctaca aggtcgattc ggatgcggcc tcgccacgcg tctgcttcca
      601 gttcaccgag gccctgcaac gcgggcgggt ggatttcgcc ccctacgtcg ccgtcaccgg
      661 tgccgcctct ttcgcggttt cggcggagga tcgccagctg tgcgtcgacg ggctgaagca
      721 cggcgagcgc tacaccatga tcgtgcggca gggactgcca tcgtccgtcg gcgaggccct
      781 gctgaagacg gctgactatg aaatctacgt gcgggatcgc tcgccgcagg tgcggttcac
      841 cggcaagaac tatgtattgc cgcgcgtcgg tcaggagggc atcccggtcg tctcggtcaa
      901 tgcgcagaag gtcaatgtcg atatcgcgcg cgtcggcgat cgctcgctgt tgccgaccgt
      961 ccgttccgaa gagttcctga atcagatttc cagcggccag gcgaagaagt tcgccaacga
     1021 gaccgggttc aagatctggt ctgggacgct cgacatcaag agtgagcaga acaaggacat
     1081 cacgaccgcg ttccccgtgc tggaagcggt cggcaagctg gagcccggcg tctacgtgat
     1141 gacggcctgg ccggaaagcc tgaacgcgcg atcgtccggc agcgggaacg gcgatgatga
     1201 cgattattcc gatcgctcga cccagtggtt cgttgtttcc gacctcggcc tgacagcagt
     1261 cagcgctgcg gatggcgtgc acgtcttttc tcgttcgctc gccagttcca aggcgcttgg
     1321 cggcgtcgag atgcggctcg tcgccaagaa caacgagatt ctcgccacgc agaagacgga
     1381 ttctcgcgga tacgtgaagt tcgatcccgg actgtcgcgc ggcatgggcg ggcttgagcc
     1441 cggcatggtg gtcgcgaccg acgccggcgg tgactacggg ttcctggatc tgcagcaggc
     1501 cgccttcgat ctcaccgatc gcggcgtgaa gggccgtgcc gcgcccggcc cgctcgatgc
     1561 gttcgtcttt gcggaacgcg gggtctaccg ctcgggcgag accgtgcagc tgaccaccct
     1621 gttgcgcgat gcgaagggcg tggccgccgg agggctgccg ttgacgctgg tggcccagag
     1681 gcctgacggt gtcgaataca agcgggttcg cgtcgaagat cagggccttg gtggcagggc
     1741 gtattccctg gcgcttctgt cgggggctcc gcgcggtacc tggcgcgtga aggcctatgc
     1801 tgaccccaag ggcaagccga tcggggaaac cagcttcctg gtcgaagact atgttcccga
     1861 acggctcgac gtggatctca agccgaagga gccgctgctg cgctcggggc aaccgacgga
     1921 agtcgacgtc agcgtcaact atctgtacgg cgcacccggc gcggaccttg aggtcggcgg
     1981 cgaggttacg gtccgtcttg ccggcgcgac cacggtcccc ggcctgaagg actatgtcac
     2041 cgggctcgac gacgagacct tcgaatcgac cacttcggaa ctggaagagc gcgccaccac
     2101 cgatgccaga ggcagggcga cggtgtccgt cagcatcccg gaggccaccg ccccgcgtcc
     2161 cttggaagcg cgcatcgtgc tgcgggtcgg ggagagcggc ggccgcgcgg tcgagcgcag
     2221 tgtggtgttg ccgatccggc caaatggtcc ggttctggcc gtgaagaagc tgttcagcga
     2281 cgattcgctg tccgaaggct cgatcgccac gttcgatgtg gtggccgcca cggcagaggg
     2341 cgaaaagctg ccgcgcaacg gtgtgcaatg gacgctgtcg cgcgtcgaac ggcgctacca
     2401 gtggtacaat tccgatggac gctggagttt cgagccggtg aagtccacgc gccgcgtcgc
     2461 tgacggacgc atcgacgtca ccgcggcggc tcccggccgg atttccgcac cggtcggctg
     2521 gggcacctac cgcctcgagg tccgcgccga cggttcgaac cttgcgccgg tcgccaccac
     2581 cttcacggtg ggctggtccg gcgaatcgac cgcgaccacg ccggacctgc tcgaatcctc
     2641 gctggacaag ccgagctaca agcccggcga ccggttgcag gtaaagctca atccgcgctt
     2701 tgccggcacg gcgacggtga tgatcgtcgg cgatggcgtg cacgatgtgc agctcgtcga
     2761 cgttcccaag gagggcagca gcatcaccat cccggtcaag gccgaatggg gcgctggcgc
     2821 ctatgccgtc gccttcgcgc atcgtccgct ggatcaggcg gcgcaacgca tgccgggtcg
     2881 tgctctgggc gtagcctggt tctccatcga tcagccggcg caaaagctcg ccgtcgaact
     2941 tggcacgccc gaaaagatca ggccgcgcgg aatgctgtcg attccggtca agcttacggg
     3001 tgccgcgccg ggcgatgaag cctatgtcac agtggccgcc gtcgatgtcg gcatcctcaa
     3061 cctgaccggc tatgaactgc cggatgcttc cggccattat tttggccagc gccaactcgg
     3121 cgccgatatc cgcgacctct atggattcct catcgacggc atgcagggca cgcgcggggc
     3181 aatccgctcg ggcggcgacg ggggcaagcc gagtctcgac ggcgcgccgc cgcagcaggt
     3241 gccgatggcg cgctactccg gcgtggtcaa ggttggcacc gacgggaccg cgcaggtcga
     3301 attcgacatt cctgccttca atggcaccgc gcgcgtcatg gccgtggcct ggacccggag
     3361 cggtgttggg cagaccaaga aggacgtgat catccgcgat cccgtggtga tcgccgggac
     3421 actgccgcgc ttcctcaact atggcgacga atcgcgcttc ttcctgcaga tcgacaacat
     3481 cgagggtggc gctggcgact acacgcttga tcttgacttg cgggggccgg tgatcgtcgc
     3541 cgccgatgcg gcgcggcgca ccgtacgcct caatccgggc ggcaagacct cgctctcgat
     3601 tccggtgaaa gccggcggcc tcggcacggc gaccttcgac ctgcgcatga caggccctgg
     3661 tgtcgatgtg acgcacagtt tcaacgtcac ggtactgccc ggcacgccgg aagtgttcca
     3721 gcggtcggtg cgcatgctta atccgcagca gaatgtcacc ctgtccagcg atcttctggc
     3781 ggaatacctg cccaagacgg gctacctctc ggtcgccgtg tcgccgttcg ggggcatgga
     3841 tgtgcccgcg ctcctgaggg cactcgaccg ctatccctac ggctgttcag agcagatcgt
     3901 cagccgcgcc atgccgttgc tctacgtcaa taggctggcg cgggcggaag ccctctcgct
     3961 tgatccgaac ctggacgagc ggatcaagga cgccatcgaa cgcgtgctgt cgcggcagga
     4021 ttccagcggg gccttcggcc tctgggccgc cggcggcgcc gatgaccact ggctcgacag
     4081 ctacgtcatg gatttcctga cgagggcgcg cgaacgcggg ttcgtcgtgg cgcagaagtc
     4141 cttcgacctg gcgctggatc gtctgcgcaa ccatgtcgcg aacacgtccg aggttcggga
     4201 cggcggccgg gatctggcct atgccatcta tgtgctggcc cgcaacggtc gtccggtgat
     4261 gaacgacctg cgctatctcg tcgataccaa gctttccgat ttcacgacgc cgctggcccg
     4321 agcccagctc gcggcggcgc tggcgctgct tggtgatcgc gggcgggccg gatcggtgtt
     4381 ctccaacgcg gcggaggaat tgtccaagga caagatgagc ctgtcctcac gctcggacta
     4441 tgggtcgaag ctgcgcgacg gtgcgggtat tctgaccctc gcggccgagg ccaacctgtc
     4501 gcgcgacgta ctggtcaaga cagcggccgt ggttttcgat acgcggcagg ctacgcgcta
     4561 tacctccact caggaaaacg cctggatggt gctggcggcg caggcgcttg tcgcggatgc
     4621 gcaggcgatg gcgcttaccg tcaacggcga ggcgcacaag ggcgcgctct atcgcaccta
     4681 cagcgccgaa cggcttgaag cccggccgct cacaatcgag aacgccggac aggcgcccgc
     4741 gcatctcgtg gtctccgctt cgggcaatcc cctggtgcag gaaccggcgc tcgcgcaggg
     4801 ttaccgggtc gaacgcgacc tgcacaagct cgacgggacc aaggtcgatc ccacgaagat
     4861 caagcagaac gatcgcctcg tcgtcgtcct caaggtcacc gaactggagg cgcggtttgc
     4921 ccgcctgctg ctggtggaca tgctgccggc gggactcgag atcgacaatc cggaactggt
     4981 ggagggcggt tcgctcgagg gcttcagctg gctgaagcgt gaggcggaac cggtgaacac
     5041 cgaataccgg gatgatcggt ttgtcgcggc gttccatcgc agtggcgaga agacggccga
     5101 attctctgtc gcctatctcg tgcgcgcggt tgcgcccggc gcctatgtgc atcccccggc
     5161 ggtgatcgag gacatgtatc gtcccgaacg ctttgggcgc acggcctacg ggcgggtcga
     5221 ggttgcgccc gcgcggaact gacatggtca gggacgaaac gtcgcgcctg cgccgctatg
     5281 gccgccgtct ggcctgggtc ggggtggcag ctgtcgccat cgtggcgctg gcgacagccg
     5341 gctacaggac ggcgctgaac cggcttggtc cactggatct tgcggctgtc ggggatgggt
     5401 ccccggttgt ccttgatcgc gccggcaagc tgctgcgccc gttcaccacg gccgatggac
     5461 gctggaaact gccggttgcc gcagcggaga tcgatccgct gttcttccgg ctgctgaaga
     5521 cgtacgagga ccggcgcttc gacaggcatg gcggcgtcga tgtcctggcc atggcgcgcg
     5581 cctccctcca ggccatccgc agcgggcgtg tcgtctcggg cggctcaaca ctgaccatgc
     5641 aggtcgctcg cttgctggag ccacgcgacg aacggacggt gccggcaaaa gcgcgacagg
     5701 tgctgcgggc ctggcaactg gaagagagcg ccagcaaaca gcgcatcctc gactattatg
     5761 tggtgctggc accctatggc ggcaacatcg aggggctgcg cgccgcaagc atcgcctatt
     5821 ttggcaagga accgcgccga ctgtctctgg ccgaggccgc gctgctcgtg gcgctgccgc
     5881 aatcgccgga agcgcggcgg ccggaccggt tcgccgagac tgcccgcaag gcgcgcaacc
     5941 gcgtgatcga cattgccgtg gcgcgaggca cggtttccgc cagcgacggg gagagcgcca
     6001 agggcgagcc ggttccccat cagcgcaagg cgtttccgat gttggctccg cacgccgccg
     6061 aagcggccgt ccgggcacgg ccgggcgagc gcgtccatcg cctgagcatc gatgcgcgct
     6121 tgcaggcgag cctcgaaccg atcgcgcgcg agcgggtgga ggcgctggga gcgaaagtcg
     6181 cggcggcgat cctggtggtc gacaatgcaa ccggcgaggt gcgcgcgcat gtcggcggca
     6241 gcgagtacct gagcgcccag cgggcgggcg gcgtcgatct gatgcaggcc gtgcgttctc
     6301 ccggttcggc gctcaagccg ttcatctacg gcctggcatt cgatgcgggc atcgcgcatc
     6361 ccgaaacgca acttgaggat cgcccctcgc ggttcgcgac ctatgccccc gagaacttcg
     6421 acatgacctt ccagggaacg gtcacggccc gcaaggcgct ccagcagtcg ctgaacgtgc
     6481 cggccgtcga actgctcgcc gaggtcgggc catcgacctt catcacccgg ctgcgcagcg
     6541 ccggtgccga tatcgtcctg ccgaaggatg ctgtcgccag ccttgccgcg gcgctcggcg
     6601 gtctcggcat ccggccggtc gacatggcca agctctatgc cgggctggcg cgtggcggca
     6661 tgacccagcc gctcacatgg cggcttggag aaggcgcgcg gccggagcgg gagcggcggc
     6721 tgctggacgc cgtctcaagc tggtacctgg ccgatacgct gcgcggcacg ccgccaccga
     6781 caaacgccct gtcaaaccgc atctcgttca agacgggcac gtcatacggc tatcgcgacg
     6841 cctgggccgc gggattcgac cgacgcacga caatcgtggt ctgggtcggc aggcccgacg
     6901 gtgcgcccgt gccgggattg accggtcgca gcgccgccgc cccgatcctg ttcgatgcct
     6961 tctcgcgcct cggcggcgaa aacgaggtca tcccggcccc acgcaatgtg ttgatggcgg
     7021 cgaccacgca gctgccgccg cccctccagc gcctccggaa ggatgcgcca aagacgctgg
     7081 ccgccgccgc cccttccctg aagatcgcct ttccgcccga tggcgcgcgc attgatctcg
     7141 gcttcgcagc cggcagggag cgcagcgatc tcgtgctcaa ggccaacggc gggcaacttc
     7201 cgctgatctg gatggtcaac gggcgtccgg tgtcgcaggg cgagttgcgt cgccaatcga
     7261 cctggcagcc agacggcgcg ggatttgcgc gcgtgtcggt catggatgct gccgggtcgg
     7321 tggattccgt cacggtgcgc gtggaatagt ggctggcgca cgaaggccat tgtcagcgct
     7381 gtggcggctc ctgtagttat catacaagtt cgggcgcagt cgttgcgctc atgggcgtgg
     7441 ggagtgcggg atggcgttgt caaaagtcgc ggtgatcacc ggagcgggct cgggcgtggg
     7501 caaggccggc gcgctggcgc tggcggggga aggtttcgcc attgtgctcg cgggacggcg
     7561 caaggagcag atcgacgccg tcgcggccaa gatcgcgagc aaaggcggcc aggcgctggc
     7621 ggtcgtgacc gatgtcaccg atccgcagtc ggtggagcgt ctgttcgccg aggcgaaacg
     7681 ggcattcggc cgtgtcgatc tgctgttcaa caacgccggc atttcggccg gcgggcctat
     7741 cgaggatctt tcggtcgagg actggaagcg ggtggtcgac accaatctca ccggtccgtt
     7801 cctgtgcacg cgcgaagcct tccggatcat gaaggcacag acgccgcgcg gcgggcggat
     7861 catcaacaac ggctcgatct ccgcctatgc gccgcgcccg aactccgcgc cctacaccgc
     7921 gaccaagcac gccatcaccg gcctgacacg gtccacggcg ctggacggac gcaagcatga
     7981 catcgcctgc ggccagatcg acatcggcaa tgcggcgacc gacatggcca tgcggatggc
     8041 gtcgggcgtg ccgcaggcca acggcgaaat cgccaaggaa cccttgatgg atccacagtg
     8101 cgtcgctgat gcactcgtcg ccatggcgcg catgccgccc gatgcgaacg tgctgttcat
     8161 gacagtgatg gcgacgaaga tgccgttcgt cggtcgcggc tgaacccgac cctattccgc
     8221 cggggtcttg cgctggcgtg gagagcgggc ccgccgttgg gtaatctgag tttcggcgcg
     8281 ggccttgtcg cggcgcacgg cggcgatttc cgcgtccccc tgccgtttct gctggcgtag
     8341 cgcctcgatt gtcgcggtca tgtcctgaac cacctcctga tgtgccagtt gggcacgggc
     8401 ggcgtaactc tcggtttccg cgatgcgggt gttcaggcgg gcgatttcgt tgtcgcgcgc
     8461 ctcgcgttca acgcgctcgc tgttccgctc acgcgtgatc tggccgattt cgtcgatctg
     8521 gcgcagacgc tcggcctgca actgggcgat ctgggcgccg cgttcttcgc ccagcgcttc
     8581 cgctgccgcc agccggcgct cggccaggga tttctgtgcc gtcgtctcgc gcaatccttg
     8641 catggtcgcg cgcaggtcac tggtcctggc gcgcaggtcg tcgcgcaagg cggcgataac
     8701 ggcctgctgt tcgcttgtct tctcggtcag cgtggcgagc tggcgctcca gctccgccag
     8761 acgcgccagg cgctgttcgg cgagttggcg ctgcgtcagg ctctcctgat caagctcctg
     8821 attgaacagg cgggtggcat caaggttggt gaccaggttg tcgcgctcgt tcctcaaggc
     8881 atgatagcgt tgattgagcg cggcgatgtc ctcgtgtcgc gcgacgagat cggcctgcag
     8941 tgcggcgatg ctggcgttcc tctcgccgat cgtcgcctcg cgctgttgga tggtcgcgac
     9001 atggtcgccg atttctttgc gcgcctgctg caccagcgcg tccgtcgcgt ccagacgcac
     9061 ttccaggcgg cggacctcga cggctgcggc cgctcgaatc tggtctttct cggccgccat
     9121 ctcgttcggg gtgaccggaa gggtggccat cagccgttga cgcgacaggc gttccgcgcg
     9181 ccgccagagc gccggcaaca gggcaatcgc aagcagcccc gcaagcaggg cgccgattgc
     9241 cagatacatg atcgcttcga tcatcggacc ttggagccaa tcaaaggtcg agacgatgcc
     9301 acggcgatgg cttcagcgcc agtgcggccg ctacgcggaa ttggcgtgcc ctgctcagaa
     9361 cggattccag gtgggctggt cggtgaactt cagatagccg acgttgacgc cgagacgcgc
     9421 gccgacgccc gagcggatcg ggacgatgat gacgccttcc gagccgagcg ccgtcatgcc
     9481 gaagccgccg atgaaatagg ccgagccatc gatgccggcg taacgcctga agaggttcgc
     9541 gacgctcggc aggttgtaga ccagcatcat ggtgcgtgcg ccttcaccgc cgaaatcaaa
     9601 gccaagcgac ggtccctgcc agaacacgcg cgtatcgccg gcgttgcgcg tgtagagcat
     9661 gccttccccg tagcgcaggc cgccgatgaa ggcgccggag ccctcctggc cgagaatgta
     9721 gccgttgggt tcgccccagc ggcgaaccgc ctcctcgacc gtcagggcta ggccgcgcga
     9781 cacggtgccg aagaacttgt gtccggaatc gacgagttcg cgggattcat aggtgttgac
     9841 gggtttctgc tgctgctgcg cctgtgctcc gccggctgcc agaaaggcga tgatgagcag
     9901 ggccctagcc gcctgtgcga tcatgttgct tcgtctcccg gcgctgcccg agaggccgcg
     9961 cccgccacat cttaagcgaa tcacctgtgg caagcttcgg gtccaaaccg ggcggccgca
    10021 aggcaaatgc acttggcgcg caagccggat tctcaagact gcggcaaatg tgtatcgcag
    10081 gatatcgatt tttcctgctg attcggaccg atgcgatgag cgatgttgcg agcccaccga
    10141 ttgccctgcc gattgcgctc gattctctcg atctcgcggc actgttatgc agccgggttt
    10201 gccacgatgt catcagtccg gtcggcgcga tcgtcaacgg gctggaggtg ctcgacgacg
    10261 acagtgatca ggaaacgcag gatttcgcgc tggcgctgat ccgcaagagc gcgcagcagg
    10321 cttcagcccg gctgcagttc gcccgccttg ccttcggtgc agccggttcc gcgggcgctt
    10381 ccattgatct tggcgacgcg gagcaggtca cgaagtcctt ccttcaggac gacaaggtgc
    10441 agattgtctg gcaggcgccg cgccttctgt tggccaagaa caaggtcaag ctcctgctca
    10501 atctgctggt gatcgctgcg gcatcgattg tccgcggggg cacgatcacg gtctccctga
    10561 gcgattcgga cggccagacc gccatttcga tcacagcaca gggtccgaac tcgcgcatgc
    10621 cgcctcacgc ggtcgcgctt attgccggcg agagcgcgac cggaggcatt gatgcgcacg
    10681 gaattcaggc ctattatacc gggctggtgg cgcgggcgag cggcatggcc gtcacgctgg
    10741 agcatgccgg cgacacgctg accatatccg cccgctcgac ttgagtgtca tttccggccg
    10801 gcggtatcct gctgcatcaa ccgatcgtta gccttaccga aaaccattcc tcaaccgttg
    10861 cgcatcatag tcgctgaacc gtaccgccga ttgtgcgcgg gcggcgtatg cgggcgcgat
    10921 ggacgatctt ctgtcggatt ttgcagtcga gacgctagac agcatcgatt cccttgatgc
    10981 cgagatcgtg cgtcttgagc gtgacccgtc cgatgctgcg gcgatcgagc gcattttccg
    11041 cgttgtgcat acgatcaagg gcacctgcgg attcatcaat ctgcgcaggc ttgagcggat
    11101 ttcgcacgcg ctggagaacc ggatatcgat tttccgcgac ggtgcgacgc cggatcgcgc
    11161 ggcggtcaac gctgtctttg cgacgctcga ccgcatccgg gtgattgccc gggcgatcga
    11221 gaccgtcggc gtagagccgg ccggcgacga cgaggacctg ctctccgcgc tcgtgcagga
    11281 gggcatcggc gccaccggtc gcccgagcca gggcgggcac cggtcctttg agaccggctc
    11341 cctggtctat caggtgctgg agcgtcccct tcgggagggc gaggttacgc tcgacgacct
    11401 ggagcgggcg ttccgtcagg cgccgggtcc tgagggggcg accgggacgc ccggagggcc
    11461 tgccgccggt gcgaaccatt cggcttcgac cgccaaggcg cgcgtgcgcg tggcggtgga
    11521 gcgtctcgac catctgatcg atgtcgtttc cgaactggtt ctgatccgca accagctcct
    11581 cgatctgtcg cgcaaggatg gctcgggggc ttacaagctg cccttgcaac ggctgtcgaa
    11641 catcaccggc gagctccagg atggcgtgat gcagacgcgc atgcaggcca tcagcacggc
    11701 ctgggccggt ctgccgcgca ccgtgcgtga cctggcgcta agccttggca aggacatcgt
    11761 gctcgagacc agcggcgatg acaccgaaat cgaccgccag cttctggaga tcatcaagga
    11821 cagcctggtg catctggtcc gcaatgccgc cgaccatggc atagaactgc cggctgagcg
    11881 catcgcggcg gacaaggcac cgacgggtat cattcggctt tcggcggggc gcgagcgcgg
    11941 ccagatcgtg gtgcgcatcg aggatgatgg gcgcggcctt gaccccgatg cgctgcgtca
    12001 gcgcgccgtt gcgcgcggcc tgctggatga agccgaaagc cgcgctttgg gggattcgga
    12061 agcagccttg ctgattttca ggccgggttt ctcgaccgcg tccgccgtca ccagcgtgtc
    12121 cgggcgcggc gttgggctcg atgccgtgcg tgacagcgtc gagcaggctg gtggaaccat
    12181 cgaactgctc aatcggcccg gccatggtct tgccgtgatc ctgaagctgc cgctgaccct
    12241 ggcgatcgcc aatgtgctga tcgtcgaatc cgccggccag cgcttcgccc tgccgcagct
    12301 ggcggtgacc gaactggtcc gcacgcaggc ggacagcgac gtgcgcgtcg agatgatcgg
    12361 aacaacgctg acgctgcggt tgcgcgacgg cctgctgccc ctgatccggc tcgatcaggc
    12421 gctgggccgg gaagcgcagg atgcgcctgc tggcgtcgtc gcgatttgcc aggctggcca
    12481 tgtccgcttc ggcctggcgc ttggcatcat ccttcagacc gaagaaatcg tggtgaagcc
    12541 gctgcctggg gcgctgcgct cgatacccta ctactccgga accaccattc ttggcgatgg
    12601 atccgccgtc ctcattctgg aaccggcggg atttgccagg ctcgtggaag gcgccggcgc
    12661 cagcgcgccg cccgtccccg aaagtcggcc agagacagag cggcaggcgg cgaagaccgc
    12721 ggtactggtg ttccgtggcc atgacggtcg cgagaaggcg gtgccgctgt ccctggtgaa
    12781 gcgcctcgag gacttcagcc cgcaggatgt cgagcggctc ggcgggcgtc aggtcgtcca
    12841 gtatcgcggc ggcgtcatgc acctgctcgg tgaacccggg ttgcctgaac aggcagagcg
    12901 cgaagatcgc atgccggtga tcgtcttcag ctgcggccgg gaaacggttg gcgttgtggt
    12961 ccaggaaatc gtcgacatcg tcgaggacag gttcgagctt gatctcgctc atgcgagccc
    13021 gggctggctc ggcacggcga tcatcggcgg gcggcttatg gaagttctcg atgtcgcggc
    13081 gctgttcgca gcgggcgcgg cggtggtccc ggaaatcccg gggcatggcc cgccgcgtgt
    13141 cctgctggtg gaaccaagcg ccttcttcca ggccatgctc gcgccgttgc tgcgtgccgt
    13201 cggcctggac gtcaccatcg cctcgaacgc ggacgaagcg ctggcgctcg ccgaggccaa
    13261 ccggttcgcg gtcgccatcg tcgatttcga cggagccgtc agccgtgcgg gcgaattgtg
    13321 catggcgctg cgcccggacc tcaaggcgcc tgcgcttgtg at
//
LOCUS       DAOMXN010000043        13362 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_2535_length_13362_cov_11.174044, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000043 DAOMXN010000000
VERSION     DAOMXN010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 13362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13362
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2535_length_13362_cov_11.174044"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PK812_05260"
     CDS             <1..255
                     /locus_tag="PK812_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HRJ68999.1"
                     /translation="VGASEEVDEQVDTEDEADSAGGSDSLMSEIDANGDGMISLDELM
                     NALLGRNQSGASQSGGCGGSADGGGQGDSGCGASDAGCDA"
     gene            446..619
                     /locus_tag="PK812_05265"
     CDS             446..619
                     /locus_tag="PK812_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69000.1"
                     /translation="MFPQGVEAANAAGTANGSTGSAQSDAAVQAQFDQSVSTVLASVL
                     SPLVQMVIQNCSE"
     gene            688..1119
                     /locus_tag="PK812_05270"
     CDS             688..1119
                     /locus_tag="PK812_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69001.1"
                     /translation="MYQAMTLAVDPIAGPAAKVSGRQLANVAGLDPQLAINQNSLLKV
                     GGDQIADGVRAHMANLLDLERKVKPRRAGDDSEMAITELGGSKDPAGTKVLGETQTAI
                     GGDNALQNLTDAFEYATNAALVSLVLGGVTNSITTLTTRGG"
     gene            1159..1878
                     /gene="sctJ"
                     /locus_tag="PK812_05275"
     CDS             1159..1878
                     /gene="sctJ"
                     /locus_tag="PK812_05275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion inner membrane ring
                     lipoprotein SctJ"
                     /protein_id="HRJ69002.1"
                     /translation="MKVHSRLGLVALCLFLAACNDEVYRNLSARDANEIVAVLRRSDI
                     AAVRDGDGTNFRVSVPRGDFGRAVDILSRAGLPREQFRSIGEVFPGDGLIVSPFEQRA
                     RLIFAVSQELSKTIKSISGVSSARVHVVMPETDTRQIGQNRASASVVVVHRPRVDTAE
                     LSTKIRLIVANAVPELSERDVSVAFFPAEDEVSDYAGSTGAASFGGGRNGSTLTWLLW
                     VAGAGALLMGLFTLLLRRKPS"
     gene            1875..2633
                     /locus_tag="PK812_05280"
     CDS             1875..2633
                     /locus_tag="PK812_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69003.1"
                     /translation="MTPHARQVLETMLAQARQAAAQHLQSVADAPDPALSRVIGIADP
                     ALCRRIAATPRVARRIADRLRTATADEIARARTSTLADALPTDPDAIRLADADDEMAD
                     AAASDGKAEAVPTPLPEAERLLRMIAAAIRLSSSGALLGRDEIDDALSLYGVEAVDFA
                     LQSRSDVPAWMREAAGSRSDIHRQFAASCIVCLAESLPSPASAGMIDAVQVHDPVPIV
                     GSQPRAGAERLAASAVTFAASYVPGNAADAAEMM"
     gene            2643..3206
                     /locus_tag="PK812_05285"
     CDS             2643..3206
                     /locus_tag="PK812_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69004.1"
                     /translation="MDNKIIRREETGAEPLAGPIRTFMQSPEERMQQVFADARNAGYR
                     TGHEIGSKEFLTTTAEAVKAIRDEFFALESMIGPLVLQAVQKILGALPPGDVAMAALL
                     EALRDGAGSVAVTLKVAPDDLDTMRMVWAEAIAARPELAGSIAAIDGDASLRAGEMLM
                     ETLKGRTHIGIPYQLARLRYGVLGRST"
     gene            3203..4558
                     /locus_tag="PK812_05290"
     CDS             3203..4558
                     /locus_tag="PK812_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HRJ69005.1"
                     /translation="MNSAGGQARLDSAVRRLASAIQAVQTRGPSGRLIAVSGTLLKVE
                     APLAQIGELCELRNPDGGTSMHGEVVAIDNGVAHVAPYGPLAGLSNRTEVIALGRAPS
                     IHLGNDILGQVVDAFGNTLSRFSETSLKVADAQPGWRSLHADPPDALDRAPIEKPFGV
                     GLRAIDGLVTCGEGQRIGIFGPAGAGKSMLVSQIVRAAEADVVVVGLIGERGREVGDF
                     IRRTIRPERRDRTVMVVSTSDRPPIERMQAALTATSIAEYFADLGLRVMLVVDSITRL
                     ARALRDVGLSAGEPPTRRGFPPSVFSTLPRLLERSGPRKHGSITAFYTVLVEGEASAD
                     PIVEETRSLLDGHIVLSSKLAESAHFPAIDVLDSRSRVMDMVVDKSHVADAKKVRELM
                     AKYRDVELLIQVGEYRRGSDPLADQAVDKHRAILSFLQQGPDEFTPIADTRAGLHRLA
                     G"
     gene            4568..5068
                     /locus_tag="PK812_05295"
     CDS             4568..5068
                     /locus_tag="PK812_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69006.1"
                     /translation="MDIRLTRLEVIRERKAELAHAEFQRAYRHFAAAHRHHEALVQQR
                     AAGAERSDLLTRQELGRLSHGAVTTSAIAAVHDAAERRKAALALLDEEIARALAERER
                     LRQIAQEKRQVFLTARRVAEKLGLLVDKLRDEEISLQSRLSESRADSVVKDAATGRWM
                     PLQTDS"
     gene            5041..6120
                     /locus_tag="PK812_05300"
     CDS             5041..6120
                     /locus_tag="PK812_05300"
                     /inference="COORDINATES: protein motif:HMM:NF013236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HRJ69007.1"
                     /translation="MDASADGFLKPKASPLVTPLSPRLPRLSFDDADRENALARYRGE
                     FVFRGRLGEMRARVCHRRAVETADIGFACLRAQIDGAAVNFWVPAKALSAVAFLAEPA
                     VSMGDIPEEGRALLFEYVLDDLLTAVESRGHLVAITEVANPVRNPPGDLGFWASVGQV
                     PAFPGLIEGPPVFLSQLQRAADALPMRSLGFVDLPIVLRMLAGATWLSNDGYVGLRQG
                     DTVLIDRTWLTQHRAPVLVGDSLILSANVTSRGFALDRSGPVTDLRERATWLADDLIS
                     EGAFMSDGEAGNLETLEVKLVFEIGRFTMSLAELNRLDAGHVFELNRAPDQAVDIFVL
                     NRQVGSGELVMVGDRIGVRLTKLNR"
     gene            6126..6773
                     /gene="sctR"
                     /locus_tag="PK812_05305"
     CDS             6126..6773
                     /gene="sctR"
                     /locus_tag="PK812_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007636018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctR"
                     /protein_id="HRJ69008.1"
                     /translation="MDNFNVVGVFAVVLLTGLAPMLVMMLTSFAKISVVLFIVRNALG
                     IQQTPPNILLYGLALVLTAYVMMPVGREVYAVAADPRANYSSVAGIERTVIEAGQPVR
                     TFLMKHTKPRDRQFFVDAAQKVWKDARQPTPHEADFIVLIPAFISAELTRAFEIGFLL
                     YLPFVMIDFAVSAILVAVGMQMMSPTVISTPFKLLLFVILEGWSYLLQGLILSYA"
     gene            6783..7052
                     /locus_tag="PK812_05310"
     CDS             6783..7052
                     /locus_tag="PK812_05310"
                     /inference="COORDINATES: protein motif:HMM:NF013480.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HRJ69009.1"
                     /translation="MSSTHLAYEVYSSIWAAFVVTAPFLIAAIVIGFVLGLIQAVTQI
                     QDQTLPQLVKILVLSLMLVVTGGWLSSPLVDHSRRLFTNFPVWVR"
     gene            7052..7867
                     /locus_tag="PK812_05315"
     CDS             7052..7867
                     /locus_tag="PK812_05315"
                     /inference="COORDINATES: protein motif:HMM:NF013478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HRJ69010.1"
                     /translation="MPRFAGLGDGFFELATLFTMAATIGFARYYGFTFVFPLFSWMQV
                     RGPLRFAIALGLSMPSIILVYGQLRSGGLVPTGMWAVLVAKEALVGALIGALMGLPFW
                     AMQGMGDAIDVYRGGSAANLFDPVHAQEMTITGNFLLLLALVLFVVIGGVSQSVDIVL
                     RSQAVWPAMHLVPPFEWEALRNLADVAARGLSIALIVAAPLLIAMLLIDVALMFAVKG
                     ARTLQIYELSQAARGLLLLMILPAFVLLFESHFVTYLRGLLEAMRSALGLLSR"
     gene            7872..8933
                     /locus_tag="PK812_05320"
     CDS             7872..8933
                     /locus_tag="PK812_05320"
                     /inference="COORDINATES: protein motif:HMM:NF013479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HRJ69011.1"
                     /translation="MAPRDTGEERNIWPTQRRLQRLRREGVVPRSRDFQTSISLLAVV
                     VYLVLGWRSISNRLQAAFLIVDPSMPGGFSAEYHRVVSGLFKLATEVFLPIALVGITG
                     ALAGAIIDGRGLPVQSKALAPNFQRLSPAEGLKNLFSLRNLLDFGKGVLVALLVLGGN
                     VLLFRTFYNDLMWSPSCGGACVFKVFAYLIGGSILIGLIIVLAIAFIDLPLARWQFLR
                     ENKMSVSEYKRELKEEEGDPLIRRARRQQILRASQSSEFIGVHRAQIVLAFGDSAVAL
                     TYLAGKTAAPIVAARTRTDGAELVRRGAELHLPIVQEPTLVALILESANVGDFVPNAT
                     FDEVARVLVREGIIRPPQQ"
     gene            complement(8958..11063)
                     /locus_tag="PK812_05325"
     CDS             complement(8958..11063)
                     /locus_tag="PK812_05325"
                     /inference="COORDINATES: protein motif:HMM:NF012973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HRJ69012.1"
                     /translation="MGRVLNAILAQLAARQDLIFLAFIFVFVLMFIVPIPPGVLDVMI
                     TLNIALTLIVLVAATYLRGPTDLSTFPAIILFTTVFRLAITVASARLILAYADAGDVI
                     KTFGNFIIGGKLLVGLVVFLIVAVVQFIVVVKGAERIAEVGARFTLDALPGKQMSIDS
                     DLRNGDISKEEARHRRAMLEKESQFFGAMDGAMRFVKGDAIAGFVVIFVNLLGGILVG
                     MATRGMGFSEASRTYSLLTVGEGLVSQIPAMLVAIAAGIVATRVAREDSSGLGADIAS
                     ELAANKRSLVIAGAVLFLLGFMPGFPTLIFATIAGLLVVAAFLLSPEWMFWRKPAPVE
                     DSGAVDESVPFVEQTEPQPTWAPADVGDVYQIVVPAAVAQAIDEAGVLPAWQERVAAM
                     DRDFGFSLARASLRVDDTLPAGTYRLDVEGVPVFWGAWRPDMRTIFASRPEAEQLGFA
                     SGWSDHSVLGPIAEVPSGALPAAAPADRTMDAAQRFILEMEYSIRAHAHASFGFAEAS
                     AWLDQLAPKYPQLVAQVHQTVPTLRLVEICRRLLDEGVSLSPPRTLLEALLRSHDGKQ
                     DVTATVEAVRKLLRRQIIYAITRGAPALPAILLDPNAESRLIAMSRMAAADLEGQKAS
                     GDLSGLTFARVVGTAANDARNQATSPVLVTTSKVRAIAQAMLKRHGVQIPVVSLDEIG
                     TETQVRPLRALTVAELGLA"
     gene            complement(11066..12106)
                     /locus_tag="PK812_05330"
     CDS             complement(11066..12106)
                     /locus_tag="PK812_05330"
                     /inference="COORDINATES: protein motif:HMM:NF028003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscD/YscD/HrpQ family type III secretion system
                     periplasmic domain-containing protein"
                     /protein_id="HRJ69013.1"
                     /translation="MAAVQDFTSTLARLRPALAGTRTGVRARVEILSGGTLAGYRDLP
                     EGVYRIGARSDCDLVVPEAKTPHIATLKIEKDAAGRWTLSLVPFIEGMQLEGKPLAAF
                     NPVKLVGASALRLDQTTLRISPLGIAGTLGDIARSAGQKVREGAASGHLGSLQGPPVA
                     RVDLPRLPEIKARPLLLVAGFLFLAALLSSFWDAFGASVLRPILGGGGTTTETRIEDA
                     GDTLKLVRQQLATADLADHVKARLDGKAVLLEGTLTERQEDRYRALLPGLRRRTAVEI
                     LSHVRPVALPLHSQIAGVALEPVPMLIMQGGERFQVGDTLPQDWRVEAITAQGIVLSR
                     GALRETIPLGGR"
     gene            complement(12261..>13362)
                     /locus_tag="PK812_05335"
     CDS             complement(12261..>13362)
                     /locus_tag="PK812_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017264449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRJ69014.1"
                     /translation="QAAARAPAQVEDAERGPDDLAAILYTSGTTGRSKGAMLSHANLA
                     SNSLTLKEYWRFTDRDVLIHALPIYHTHGLFVATNVTLMAGATMLFFARFDPNQIMAA
                     MPRATAMMGVPTFYTRLLQHPGLTKEATGGMRLFVSGSAPLLAETHREWQARTGHAIL
                     ERYGMTETNMNTSNPYEGDRRAGTVGFPLPDVALRIADPETGAVLKQGEIGMIEVKGP
                     NVFKGYWRMPEKTAAEFRGDGFFITGDLGMIDERGYVSIVGRGKDLVITGGFNVYPKE
                     VEAEIDGMPGVIESAVIGIPHPDFGEGVTAVVVRQKGSALDETEILGRLSERLAKFKQ
                     PKRIIFMDDLPRNTMGKVQKAVLREQFKSLYS"
BASE COUNT         2184 a         4277 c         4626 g         2275 t
ORIGIN      
        1 gtgggggcga gcgaggaagt cgacgaacag gtcgatacgg aagatgaggc agacagcgcc
       61 ggcggttccg attcgctgat gtccgagatc gatgcgaatg gcgatggcat gatcagcctc
      121 gacgagttga tgaatgccct gctcgggcgc aaccagtccg gcgccagtca gtccggtggc
      181 tgcggaggat cggccgacgg cgggggacag ggtgacagcg gctgtggcgc cagcgacgcg
      241 ggttgcgacg cctgatgctc ctgttggggt ctggcaggcg ggacaggacc tggcaggctg
      301 attgctgacg ggggcttcag ggcgtgcgcc tgtcgagtaa cgggacggtt gcggcacggc
      361 gtccgcgcgg acatccggcg ttcatggtcg gatgtgttgc tgatgggtta atgcaggcct
      421 gaacacaggc acgggagttg gaagcatgtt tcctcaaggc gtcgaagcgg ccaacgccgc
      481 aggcacagcg aacggcagca cgggcagcgc ccagtcggat gcggcggtgc aggcgcagtt
      541 cgaccagtcg gtgagcaccg ttcttgcatc ggtgctgtcg ccactggtcc agatggtcat
      601 tcagaactgc agcgagtgag tttctgaccc gctctgatcc gaaactgtcc cgagcctggc
      661 accgacacgt ttcaacggga gacgagcatg taccaggcaa tgacgcttgc cgtggatccc
      721 atcgcaggcc ctgccgccaa ggtctccggc cggcagctgg cgaatgtcgc cgggctggac
      781 ccgcaactcg cgattaacca aaacagtcta ttgaaggtgg gtggcgacca gatcgcggac
      841 ggcgttcgtg cgcacatggc caacctgctt gatctcgagc gcaaggtgaa gccaaggcgc
      901 gcaggcgacg acagcgagat ggccatcacg gagctcggcg gcagcaagga tccggctgga
      961 accaaggtgc tcggcgagac acagaccgcc atcgggggcg acaatgccct ccagaatctg
     1021 actgatgcct tcgagtacgc caccaacgcg gcgctggtaa gcctggtgct gggtggcgtg
     1081 acgaacagca tcacgacgct gacgacgcgc ggcgggtgac cgtttcgctg cctgcgtcgc
     1141 accgaagggg acgcaggggt gaaggttcat tcgcgtttag gcctggtggc gctctgcctg
     1201 ttcctggccg cctgcaacga tgaggtctat cgcaacctct ctgcgcgcga tgcaaacgag
     1261 atcgtcgccg tgctgcgccg cagcgacatc gccgccgtgc gcgacggtga tggcacgaat
     1321 ttccgcgtca gcgttccgcg cggcgacttt ggccgcgccg tcgacatcct gtcgcgcgcc
     1381 ggactgccgc gcgagcaatt ccgttccatc ggcgaggtgt tccccggcga cgggttgatc
     1441 gtctcgccct tcgagcagcg cgcgcggctg atcttcgccg tcagccagga attgtcgaaa
     1501 accatcaaat cgatcagcgg ggtgtcctcg gcgcgcgtgc acgtggtcat gcccgagacg
     1561 gatacgcggc agatcggaca gaaccgcgcc tcggccagcg tggtcgtggt gcatcgtccg
     1621 cgcgtcgata cggccgaact gagcacgaaa atcaggctca tcgtcgccaa tgcggtaccc
     1681 gaactgtccg aacgcgatgt gtccgtggcg ttcttcccgg cagaggacga ggtatcggac
     1741 tacgcggggt ccaccggcgc ggcatcgttt ggcggcggcc ggaacggcag cacgctgacc
     1801 tggttgctct gggtggccgg ggcgggcgcg ctgctgatgg gactgttcac gctgctcctg
     1861 cgccgcaagc cgtcatgacg ccgcacgccc gccaggtgct cgaaaccatg ctggcgcagg
     1921 ccaggcaggc ggctgcccag catctccaga gcgtggccga tgcccccgat cccgcgctca
     1981 gccgtgtgat cggcatcgcc gatccggccc tgtgccggcg tatcgcggcc acgccgcgcg
     2041 tggctcgccg gatcgccgac aggcttcgga cggcaacggc ggacgagatc gcccgggcgc
     2101 ggacgagcac gctggcggac gcgttgccca ccgatcccga tgcgatccgg ctggcggacg
     2161 cggatgacga gatggcggat gcggcggcca gtgacggcaa ggccgaagcg gtgcccactc
     2221 cgttgcccga ggccgagcgc ctgttgcgca tgatcgccgc cgcgatccgc ttgagttctt
     2281 ccggcgcgct gctcggccgc gacgagattg atgatgcgct gtcgctctat ggcgttgagg
     2341 ccgtggattt cgcgttgcag agccgttccg acgtgccggc ctggatgcgc gaagcggccg
     2401 ggagccgcag cgacatccat cgccaatttg cggcgtcctg tatcgtctgc ctggcggaaa
     2461 gcctgccctc gccggcttcg gccgggatga tagacgcggt gcaggtccat gatccggtgc
     2521 cgatcgtcgg cagccagccc agggccggcg cggaacggct tgccgccagc gccgtgacct
     2581 tcgccgcctc ctatgttcca ggcaacgccg ccgacgcggc cgagatgatg tgaggccatc
     2641 ccttggacaa caagatcatc cggcgcgagg aaaccggggc ggaaccgctg gccgggccga
     2701 tccgcacgtt catgcaaagc ccggaagagc ggatgcagca ggtcttcgcg gacgcgcgga
     2761 acgccggcta tcgcacaggg cacgagatcg gcagcaaaga gttcctgaca accacggccg
     2821 aagccgtgaa agcgatccgc gacgagttct tcgcgctgga atcgatgatc ggtccgctgg
     2881 tgctgcaggc cgtccagaag atcctgggcg cgctgccgcc gggcgacgtg gccatggccg
     2941 cgttgctgga ggcgttgcgg gacggggccg gcagcgtcgc cgtcacgctg aaggtcgcgc
     3001 ccgatgatct cgacacgatg cgcatggtct gggcggaggc gatcgcagcc cgacccgagc
     3061 ttgccggcag catcgccgcc atcgacggag atgccagtct cagggccggc gaaatgctga
     3121 tggaaacgct gaagggccgc acgcatatcg gtatcccata ccagctcgcc cggttgcgct
     3181 acggcgtgct cggacggtcg acatgaacag tgccggggga caggcgcggc tggactcggc
     3241 ggtgcgccgg ctcgcctcgg ccatccaggc cgtgcaaacc cggggaccct cgggacggct
     3301 catcgcggtg tcgggcacgc tgctcaaggt cgaggcgccg ctggcgcaga tcggcgaatt
     3361 gtgcgagctg cgcaatcccg acggcggcac gtcgatgcat ggcgaggtgg tcgccatcga
     3421 caacggcgtt gcccatgtcg cgccctatgg accgctggcg gggctgtcga accggaccga
     3481 ggtgatcgcg ctggggcgtg cgccgagcat ccatcttggc aacgacattc tcggccaggt
     3541 ggtcgatgcc ttcggcaaca cgctgtcgcg gttctcggaa acatcgctga aagtggctga
     3601 cgcgcaacct ggctggcgct cgctgcacgc cgatccgccc gacgcgctgg accgggcgcc
     3661 catcgagaag ccgttcgggg tcggattgcg cgccatcgat gggctggtca cctgcggcga
     3721 agggcagcgc atcggcatct tcggcccggc gggcgccggc aagtcgatgc tggtctcgca
     3781 gatcgtgcgg gcggcggaag cggacgtcgt cgtcgtcggt ctgatcggcg aacgcggccg
     3841 cgaggtgggt gatttcattc gccgaacgat ccgtccggaa cggcgcgatc gtaccgtgat
     3901 ggtggtgtcg acctccgacc gtccgccgat cgagcgcatg caggccgcgc tcacggccac
     3961 gagcatcgcg gaatacttcg cggatctcgg cctgcgcgtc atgctggtgg tcgacagcat
     4021 cacccgcctt gcgcgcgcgc tgcgcgatgt cggcctgtcg gctggcgaac cgccgacacg
     4081 gcgcggcttt ccgccctcgg tgttctcgac gctgccacgc ctgctggaac ggtccgggcc
     4141 gcgcaagcac gggtccatca ccgcgttcta caccgtgctt gtggagggcg aggcctccgc
     4201 cgatccgatc gtcgaggaaa cccgttcgtt gctggacggg cacatcgtgc tgtcgagcaa
     4261 actggcggag agcgcccatt ttccggccat cgacgtgctc gacagccgct cgcgcgtcat
     4321 ggacatggtg gtcgacaaga gccatgtcgc ggacgccaag aaagtgcgcg aactgatggc
     4381 aaagtatcgc gacgtcgagc tgctcatcca agtcggcgaa taccggcgcg gcagtgatcc
     4441 gctggccgac caggcggtcg acaagcaccg cgcgatcctg tcgttcctgc agcaggggcc
     4501 ggacgaattc acgccgatcg ccgacacgcg ggccggactt caccggcttg cgggatagga
     4561 cccggacgtg gatatccggc tgacgcggct tgaggtcatt cgcgaacgca aggcggagct
     4621 ggcgcatgcg gagttccagc gcgcctatcg tcacttcgcc gccgcgcatc gccatcacga
     4681 ggcgctggtg cagcagcggg ccgcgggcgc cgagcgttcc gatctgctga cccgtcagga
     4741 gcttggcaga ctgtcgcacg gcgcggtcac caccagcgcc atcgcggccg tgcatgacgc
     4801 ggccgaacga cggaaggcgg ccctggcgct gctggacgag gagatcgcgc gggctctcgc
     4861 ggaacgcgag cgactcagac agatcgcaca ggaaaaacgg caagtgttcc tgaccgcgag
     4921 gagagtggcc gaaaagcttg gattgctggt cgacaagttg cgcgatgaag agatttcgct
     4981 acagagtcga ctgtccgaat cgagggcgga ttcggtcgtc aaggacgcag caacaggtcg
     5041 atggatgcct ctgcagacgg attcctgaaa cctaaggcgt cgccgctcgt gacgccactc
     5101 tcgccacgtc tgccccgcct gtccttcgat gatgccgacc gtgagaacgc gctggcgcga
     5161 tatcgcggag aattcgtctt caggggacgg ctcggcgaga tgcgggcgcg ggtctgccac
     5221 aggcgcgccg tcgagacggc ggacatcggc tttgcctgcc tgcgcgcgca gatcgatggc
     5281 gcggccgtga atttctgggt tcccgccaag gcgctgtccg ccgtggcttt cctcgcggag
     5341 cctgcggtca gcatggggga tatcccggaa gaggggcggg ccctgctgtt cgagtacgtg
     5401 ctggatgatc tgctgaccgc tgtggaatcg cgaggccatc tcgtggcaat caccgaggtg
     5461 gcgaatcccg tgcgcaatcc gcccggcgat ctcgggttct gggcgagcgt ggggcaggtg
     5521 ccggcgttcc ccgggctgat cgaggggccg ccggtgttcc ttagccagct tcagcgggcg
     5581 gccgacgcgc tgccgatgcg ctcgctcggt ttcgtcgacc tgccgatcgt cctgcgcatg
     5641 ctggccggcg cgacctggct ttccaacgat gggtatgtcg ggctgcggca gggcgacacc
     5701 gtgttgatcg accgcacgtg gttgacgcag caccgggcgc cggttctggt cggtgacagc
     5761 ctgatcctct ccgccaacgt gacgtcccgg ggcttcgcgc tcgatcgcag cggtcccgtc
     5821 accgacctgc gggagcgggc gacctggctt gcggacgatc tgatctcaga aggagctttc
     5881 atgagcgatg gcgaagccgg aaacctggaa acgctggagg tgaaactggt cttcgagatc
     5941 ggtcgcttca ccatgtcgct ggccgagctg aacaggctcg acgcggggca tgtcttcgaa
     6001 ctcaaccggg caccggacca ggccgtcgac atcttcgtgc tcaaccgaca ggtcgggagc
     6061 ggcgagctgg tgatggtcgg cgaccggatc ggcgtgcggc tgaccaagct caaccggtaa
     6121 tcgcgatgga caatttcaac gtcgtcggcg ttttcgcggt cgtcctgctg acgggactcg
     6181 cgccgatgct cgtgatgatg ctgacatcgt tcgccaagat cagtgtcgtg ctgttcatcg
     6241 tccgtaacgc cctcggcatc cagcagacgc cgccgaacat cctcctttac ggtcttgccc
     6301 ttgttctcac ggcctatgtg atgatgccgg tgggccgtga ggtctatgcg gtcgccgcag
     6361 atccgcgcgc caactattcc agcgtggcgg gcatcgagcg caccgtcatc gaggccggac
     6421 agccggtccg caccttcctg atgaagcaca ccaagccgcg cgaccgccag ttcttcgtcg
     6481 acgcggcgca gaaggtctgg aaggacgcgc gccagccaac cccgcatgag gccgatttca
     6541 tcgtcctcat cccggccttc atctcggccg aactgacacg cgccttcgag atcggattcc
     6601 tgctctatct gccctttgtc atgattgatt tcgcggtcag cgccattctc gtggcggtag
     6661 gcatgcagat gatgtcgccg acggtgatat cgacgccgtt caagctgctg ctgttcgtca
     6721 ttctggaagg ctggtcctat ctgcttcagg gcctgatcct gtcctatgcc tgaggccgcg
     6781 ccatgagttc cacccacctg gcctatgagg tctactcaag catctgggcg gccttcgtgg
     6841 tgacggcgcc gttcctgatc gccgcgatcg tcatcggctt cgtgctgggc ctcatccagg
     6901 ccgtgacgca gatccaggac cagacactgc cgcaactggt gaagatcctg gtgctgtcgc
     6961 tgatgcttgt ggtgacgggg gggtggctgt cctccccgct ggtggaccat tcgcgccggc
     7021 tgttcaccaa ctttcccgtc tgggtgcggt gatgccccgg ttcgcgggcc tcggcgacgg
     7081 gttcttcgaa ctggccacgc tcttcaccat ggcggcgacc atcggtttcg cccgctacta
     7141 tggcttcacc tttgtctttc cgctgttttc ctggatgcag gtgcgtggtc cattgcggtt
     7201 cgcgatcgcg ctcggcctga gcatgccgtc aatcatcctg gtgtatggac agctgcgctc
     7261 gggcggcctg gttccgacgg gcatgtgggc ggtactcgtc gccaaggagg cgctggtcgg
     7321 cgcgctgatc ggtgcgctca tgggcctgcc gttctgggcg atgcagggca tgggcgacgc
     7381 catcgacgtc tatcgcggtg gcagcgcggc gaacctgttc gatccggtgc acgcacagga
     7441 gatgacgatc accggcaatt tcctgctgct tctggcgctt gtgctgttcg tggtgatcgg
     7501 cggcgtcagc cagagcgtgg acatcgtcct gcggtcgcag gcggtctggc cggcgatgca
     7561 cctggttccg ccgttcgagt gggaagcgct gcgcaacctc gccgacgtgg ccgcgcgcgg
     7621 cctgtcgatc gccctgatcg tcgccgcgcc gctgctgatc gccatgctgc tgatcgacgt
     7681 ggcgctgatg ttcgcggtca agggcgcgcg cacgctgcag atctacgaac tgtcccaggc
     7741 cgcgcgcggg ttgctgctgc tgatgatcct gccggccttc gtgctcctgt tcgaaagcca
     7801 cttcgtgacc tacctgcgcg gcctgctcga agcgatgcgg tccgcgctcg gcctgctgtc
     7861 gaggtaaggc ggtggctccg cgcgacacgg gcgaagaacg caacatctgg ccgacccagc
     7921 gacggctcca gcgcctgcgg cgcgaaggcg tcgtgccgcg cagccgcgac ttccagacct
     7981 cgatctcgct gctggcggtg gtggtctatc tggtgctggg ctggcgctcg atctccaacc
     8041 gcctgcaggc ggcgttcctg atcgtcgatc ccagcatgcc gggcgggttc tccgccgaat
     8101 accaccgcgt cgtcagcggc ctgttcaagc tggcgacaga ggtgttcctg cccatcgcgc
     8161 tggtcggcat caccggcgcg ctggcgggcg cgatcatcga cggacgcggg ctgccggtgc
     8221 agtccaaggc gctggcgccg aacttccagc gcctgtcgcc ggcggaaggg ctgaagaacc
     8281 tgttctcgct gcgcaacctg ctggacttcg gcaagggcgt gctggtggca ctgctggtgc
     8341 ttggcggcaa tgtcctgctg ttccgcacgt tctacaacga cctgatgtgg tcgccgtcct
     8401 gcggcggggc ctgtgtgttc aaggtcttcg cctacctgat cggcggctcc atcctgatcg
     8461 ggctgatcat cgtgctcgcc atcgccttca ttgatcttcc gctggcgcgc tggcagttcc
     8521 tgcgcgagaa caagatgtcg gtcagcgagt acaagcgcga gctcaaggaa gaggagggtg
     8581 acccgctgat ccgccgcgcg cggcgccaac agatcctgcg cgcctcgcaa tcctcggagt
     8641 tcatcggcgt ccaccgcgcg cagatcgtgc tggccttcgg cgattccgcc gtggcgctga
     8701 cctatctggc gggcaagacc gccgcgccca tcgtggccgc gcgcacgcgg acggacggcg
     8761 ccgaactggt gcggcgcggg gccgagctgc atctgccgat cgtgcaggag ccgacgctgg
     8821 tggcgctgat cctggagagc gcaaacgtcg gcgatttcgt gccgaacgcc acgttcgacg
     8881 aggtggcccg cgtgctggtg cgggagggca tcatccggcc gccgcagcag tgatgcgcgg
     8941 cgcaggcgat ccggccgtca ggcgaggccg agctccgcga cggtgagggc gcgcagcggg
     9001 cgcacctgcg tttccgtacc gatctcgtca agcgacacca cagggatctg cacgccgtgg
     9061 cgtttcaaca tggcctgcgc gatggcgcgc accttcgacg tggtgaccag aacgggcgag
     9121 gtggcctggt tgcgggcatc gttcgcggca gtgccgacca cgcgggcgaa ggtcagtccc
     9181 gacaggtcgc cgctcgcctt ctggccttcg agatcggcgg cggccatgcg tgacatggcg
     9241 atcaggcgcg actccgcgtt ggggtcgagc aggatggccg ggagggccgg cgcgccgcgg
     9301 gtgatggcat agatgatctg gcggcgcagc agcttgcgga cggcttcgac cgtcgccgtg
     9361 acgtcctgct tgccgtcatg ggagcgcagc agcgcttcga gcagggtgcg cggcggcgaa
     9421 agcgagacac cttcgtcgag caggcggcgg cagatctcca ccaggcgcag cgtcggcacc
     9481 gtctggtgca cctgggcgac aagctgcgga tacttcggcg cgagctgatc aagccaggcc
     9541 gaagcctccg caaatccgaa ggaggcatgg gcatgggcgc ggatcgagta ttccatctcg
     9601 aggatgaacc gctgggcggc gtccatggtc cggtccgctg gagcggcggc cggcagggca
     9661 cccgacggaa cttccgcgat gggcccgagc acggagtggt cgctccatcc cgaggcaaag
     9721 ccgagttgct cggcttccgg gcgcgaggcg aaaatggtgc gcatgtccgg tcgccaggcg
     9781 ccccagaaca ccggcacgcc ttcgacatcg agccgatacg tgccggcggg cagggtgtcg
     9841 tcgacccgca ggctcgcacg cgccagagag aagccgaagt cacgatccat cgccgcgacc
     9901 cgctcctgcc aggctggcag gacgccggct tcatcaatgg cctgggcaac cgcagccggc
     9961 acgacgatct gatagacatc gccaacgtcg gccggcgccc aggtcggctg cggttcggtc
    10021 tgttcgacga acggcacgga ctcatccacc gccccgctgt cctcgacggg agcgggcttg
    10081 cgccagaaca tccattccgg cgagaggagg aaggccgcga caaccagcag gccggcgatg
    10141 gtggcgaaga tcagcgtcgg aaagccgggc atgaatccga gcaggaacag caccgcgccg
    10201 gcgatcacca gggaacgctt gttggccgcg agttcgctgg cgatgtccgc gccgaggccg
    10261 gaggaatcct cgcgggcgac gcgcgtcgcc acgatgccgg cggcgatggc gaccagcatg
    10321 gccgggatct gtgaaaccag cccctcgccg acggtgagca ggctgtaggt gcgcgacgcc
    10381 tcggagaacc ccatgccgcg cgtcgccatg ccgaccagga tgccgccgag caggttgacg
    10441 aaaatcacga cgaagccggc aatcgcgtcg cccttgacga agcgcatggc gccgtccatg
    10501 gcgccgaaga actggctttc cttttcaagc atggcgcggc gatggcgcgc ctcctccttg
    10561 ctgatgtcgc cgttgcgcag gtcgctgtcg atgctcatct gcttgccggg cagggcgtcc
    10621 agcgtgaagc gcgcaccgac ttccgcgatg cgttccgcgc ccttcacgac gacgatgaac
    10681 tggacgaccg cgacaatgag gaacaccacg aggccgacga gcagtttgcc accgatgatg
    10741 aagtttccga aggtcttgat gacgtcgccg gcatcggcgt aggcgaggat cagccgggcg
    10801 ctcgccacgg tgatcgccag tcggaagacg gtggtgaaca ggatgatcgc cgggaacgtg
    10861 gagaggtccg tcggtccgcg cagataagtc gcggcgacca gcacgatcag ggtcaacgcg
    10921 atgttcagcg tgatcatcac gtcaagcacg cccgggggaa tcgggacgat gaacatgagc
    10981 acgaaaacga agatgaaggc caggaagatg aggtcctgac gggcggcaag ctgcgccagg
    11041 atggcattga ggacgcgccc catggtcagc ggcctcccag cggaatggtt tcacgcaagg
    11101 ctccgcgcga aagcacgatg ccctgggcgg tgatcgcctc gacgcgccag tcctgcggca
    11161 gcgtgtcgcc gacctggaaa cgttcgccgc cctgcatgat cagcatgggt accggctcca
    11221 gcgccacgcc tgcgatctgc gaatgcagcg gcagtgccac cggcctgaca tgcgacagga
    11281 tctcgaccgc ggtacgccgg cgcaaccccg gcaggagggc gcggtagcgg tcctcctggc
    11341 gttcggtcag ggtgccttcc agcagcacgg ccttgccgtc gagacgcgcc ttcacatgat
    11401 cggcaaggtc ggcggtggcg agctgctggc gcaccagctt gagcgtgtcg ccggcatcct
    11461 cgatgcgggt ttccgtcgtc gtaccgccgc cgccaaggat cgggcgcagc acagatgcgc
    11521 cgaaggcgtc ccagaagctc gagagcagcg ccgccaggaa caggaatccg gcgacaagca
    11581 acagggggcg cgccttgatc tccggcagcc gaggcaggtc aacgcgcgcc acaggcggac
    11641 cctgcaggct gcccaggtgc ccgctggcgg cgccttcgcg cactttctgg ccggcgctgc
    11701 gggcgatgtc gccgagtgtt ccggcaatcc ccaagggcga aatgcgcagg gtggtctggt
    11761 ccaggcgcag cgcgctggcg ccaaccagct tgactgggtt gaaggcggcg aggggcttgc
    11821 cttcgagctg catcccctcg atgaacggaa ccagcgacag cgtccagcgg ccggcggcat
    11881 ccttctcgat cttgagggtc gcgatatgcg gggttttcgc ttccggaacg acaagatcgc
    11941 agtcggaacg ggcgccaatg cggtagacac cctctggcag gtcgcgatag ccggcaaggg
    12001 ttccgccgga caggatttcc acgcgcgcac gaacgccggt ccgcgttccc gccaaagcgg
    12061 gacgcaggcg cgccagggtg gaagtgaagt cctgtacggc agccatctga cccagttgaa
    12121 tcgtgtcaac cgcgatgatc cggcatcttg gttaataaat gggcaagaat tgcgaccgcg
    12181 ccgcgatttc cggggcgatt ccgcatggtc cttcagaacc ttgcgctgcg ttcccgcgtc
    12241 tcctgaagac gcctggcact tcaggaatag agcgacttga actgctcgcg caggacggcc
    12301 ttctggacct tgcccatggt gttgcggggc aggtcgtcca tgaagatgat ccgcttgggc
    12361 tgcttgaact tcgccaggcg ctcggacagg cgcccgagaa tctcggtctc gtcgagggcc
    12421 gaacccttct gacgcacgac gactgcggtg acgccctcgc cgaagtcggg atggggaatg
    12481 ccgatgaccg cgctttcgat cacgcccggc atgccgtcga tctcggcttc gacctccttg
    12541 gggtagacat tgaacccgcc ggtgatgacc agatccttgc cacgcccgac gatcgagacg
    12601 tagccgcgct cgtcgatcat gccgagatcg ccggtgatga agaatccgtc gccgcgaaat
    12661 tccgccgccg tcttctccgg catgcgccag tagcccttga aaacattagg acctttcact
    12721 tcgatcatgc cgatctcacc ctgcttcagc acggctccgg tctcgggatc ggcgatccgc
    12781 aaggcgacgt ccggcagggg aaagcccacc gttccggcgc gccggtcacc ctcgtagggg
    12841 ttcgaggtgt tcatgttggt ctcggtcatg ccgtagcgct cgagaatggc gtggccggtg
    12901 cgcgcctgcc attcgcgatg ggtctcggcg agcagcggcg ccgatcccga gacgaacagg
    12961 cgcatgcctc cggtcgcctc cttcgtcagc ccgggatgct gcagcaggcg ggtgtaaaag
    13021 gtcggcacgc ccatcatcgc ggtcgcgcgc ggcatcgccg ccatgatctg gttgggatcg
    13081 aaccgggcaa agaacagcat ggtcgcgccg gccatgagcg tgacgttggt ggccacgaac
    13141 agcccgtgcg tgtggtagat cggcagggcg tggatcagca cgtccctgtc cgtgaaacgc
    13201 caatactcct tcaaggtcaa cgagttcgac gcgaggttcg cgtggctcag catggcgccc
    13261 ttggaccggc cggtggtgcc ggaggtgtag aggatggcgg caagatcatc ggggccgcgc
    13321 tcggcgtcct cgacctgcgc gggcgcgcgc gccgcagcct gc
//
LOCUS       DAOMXN010000044        13282 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_2561_length_13282_cov_10.108490, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000044 DAOMXN010000000
VERSION     DAOMXN010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 13282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13282
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2561_length_13282_cov_10.108490"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="PK812_05340"
     CDS             <1..373
                     /locus_tag="PK812_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="competence protein ComEC"
                     /protein_id="HRJ69015.1"
                     /translation="GMACDRQACVGRLADGRAVSVVTDAAAFAEDCERAAIIVTPLVA
                     PHACREHAEVYDRDYFATFGATYLYARNAGFRHETARNERLDRPWSPKPVTRQPDIPQ
                     APSFDMPRQPWDSQAPDETVQ"
     gene            complement(367..1092)
                     /gene="lexA"
                     /locus_tag="PK812_05345"
     CDS             complement(367..1092)
                     /gene="lexA"
                     /locus_tag="PK812_05345"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HRJ69016.1"
                     /translation="MLTRKQSELLRFIHERLTENGVPPSFDEMKEALDLRSKSGIHRL
                     ILALEERGFIRRLPNRARALEVLRLPESTSGVARPGRQRFNPSVIEGDLGKVRAPAQL
                     SAPQTYDPDEVRSIAIPVMGRIAAGTPISAIQNRSHTINISPDFLAHGEHYALEVRGD
                     SMIEAGIFDGDTVVIKKQETADTGDIVVAYLIDDEEATLKRLRKRGASIALEAANPIY
                     ETRIFGPDKVRIQGKLVSLIRRY"
     gene            complement(1208..2437)
                     /locus_tag="PK812_05350"
     CDS             complement(1208..2437)
                     /locus_tag="PK812_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020094614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HRJ69017.1"
                     /translation="MAGAPLLPVEEALRRVLDGVRTLESETVALTAAGGRTLASDLAA
                     RRTQPPWANSAMDGYAVRAADTPAGAVLTLIGESAAGQAFAGAIGPGQTVRIFTGAPI
                     PDGADAILIQENASAEGARITVAESVRPGQFVRTAGLDFAAGQTLLKAGTRLGPTEVA
                     LAAAMNHAVVPVVRRPRVAVLATGDELVLPGENPGQDQIVASNNFAIAAAAEAYGAEA
                     HLLGIATDDFASLEAAIGRARDLKADVLVTIGGASVGDHDLVQSALAKDGMTLGFWRI
                     AMRPGKPLIFGSLGSMRILGLPGNPVSSIVCTIVFIRPLIRALLGDPGAGIDPSEAAI
                     LGADMKENDLRQDYVRSRITGRQRGLPVVEPHPVQDSSMLAVLAASQALTIRAPFAPA
                     AKAGETCRMIPLRLLTA"
     gene            complement(2439..2918)
                     /gene="moaC"
                     /locus_tag="PK812_05355"
     CDS             complement(2439..2918)
                     /gene="moaC"
                     /locus_tag="PK812_05355"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003607429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HRJ69018.1"
                     /translation="MSGLTHIDPQGRASMVDVGDKAVTSRTAVAEGLVIMQPETLAMI
                     RAGAAKKGDVLATARIAGIMAAKKTHDLIPLCHPLLLTKASIDFDLDDSLPGIRVTGL
                     ARVSGQTGVEMEALTAVSVACLTIYDMAKAADRGMRIDGIRLLEKLGGKSGHWVAEA"
     gene            complement(2915..3712)
                     /gene="trpC"
                     /locus_tag="PK812_05360"
     CDS             complement(2915..3712)
                     /gene="trpC"
                     /locus_tag="PK812_05360"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016733105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HRJ69019.1"
                     /translation="MSDILAKIEITKRAEIAAAKAAVSQAEIERRAANAPAVRGFLDA
                     IRSAHAAGRHALIAEIKKASPSKGLIRADFDPPALARAYEAGGAACLSVLTDAPWFQG
                     APEYLVAARAACALPVIRKDFLYDPYQVYEARALGADCILVIMAAVDDAVAAALTRTA
                     QGLGMDVLIEVHDSEELDRALRLDSPLIGINNRNLRTFEVSLETSRSLARRIPGDRII
                     VGESGIFTHADCEDLAACGIRTFLVGESLMRQQDVTAATRDLLGIAA"
     gene            3797..4303
                     /locus_tag="PK812_05365"
     CDS             3797..4303
                     /locus_tag="PK812_05365"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRJ69020.1"
                     /translation="MVQRARPTQVYALPEPEGGRPLQLFLTYRLAALNARLNRQASVV
                     LRRSGGLKIPEWRVIALLATHGVMNGRGIGAIAGLDAGLVSRTFRALEQRGLIALRRD
                     EGDRRIAHAALTEHGRSLFLSVRPVMAARQDRLLAALTADERALVFRIIDKLAIAADD
                     VSVPEVPT"
     gene            4300..5748
                     /locus_tag="PK812_05370"
     CDS             4300..5748
                     /locus_tag="PK812_05370"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRJ69021.1"
                     /translation="MKAANTLVILSDEHQARALGCAGHPFVRTPNLDRLAARGMRFSN
                     AVTPSPICVPARAAFATGQFVHRCGYWDNSIGYDGRVRGWGHALQEHRIPVESIGKLH
                     YQKERLPTGFDAEHIPMHLYKGVGMVWASIRDPLPENRPTDKRMLGEYIGPGESDYTR
                     YDQAVTDLAIDWLKGRGSALEAPWCLYVGLVAPHFPLVAPPDFFALYPPASLPEPKLL
                     PRHGYTLHSWLQEHEDFWSQDRTLRDDDERRTAMAAYYGLTSWLDHNVGRLISALEQA
                     GLSEKTRVLYASDHGDNLGARGQWGKSNLYRESVDIPLIMAGPDVAQGVCATPVSLLD
                     LSVTILDSLGLDPATALPQADGRSLFALAREPADADRTVFSEYHAVGSNTAAYMLRRG
                     RWKYHHFVRHRPELFDLATDPEETRDLAGTAECADVLADMERRLRAICDPEAVDRAAK
                     RDQVALIERLGGKEAVFTMGKAVAGGTPPPKT"
     gene            5776..6747
                     /locus_tag="PK812_05375"
     CDS             5776..6747
                     /locus_tag="PK812_05375"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRJ69022.1"
                     /translation="MNRRILMAALLAGAGFAAAQGGALAQSKPWPQEKPIRWLIGLPP
                     AGTTDPLTRAVAEQLSRRLGQSIVVENKPGANQVIAAREMANAPADGYTLLSMGGPQV
                     YLDASVPVIGRGFDPVIRLVTQPMILAGTTDRPTPDLKSVLAAAKANPKDWAFASAGQ
                     GSSHHVAGEYVNRLAGTAITHVPYRGGGQAIQDAAGGQVPLIMIGVGPTLAHIKSGKM
                     RGYAVTTASRIASLPDVPTLQEQGFPGVDLAQWHGVAIKSGTPAEITQRLNREIREIL
                     ATPELRALIERLAAVPGEGTPEAWGAFYKDDFAKWTRLMQELKIEAE"
     gene            6833..9307
                     /locus_tag="PK812_05380"
     CDS             6833..9307
                     /locus_tag="PK812_05380"
                     /inference="COORDINATES: protein motif:HMM:NF025709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDYXXLXY domain-containing protein"
                     /protein_id="HRJ69023.1"
                     /translation="MNAPGQLEAHLPRFHRIVQLSGAALLGGGLMLWIAANWDSIGKL
                     QKFWLVGGILLAFGAIGATLAAWRTPAALGAFLAIGALFALFGQTYQTGADPWQLFAL
                     WAALGLPLAIAARHDAVFVPWMVVAFAALHLFVASNSAFFAHPTLGLLLPIWSGGFAI
                     VAVLYGFNRSPWGENSARWAVQLGALVLASLIGGVAGAAIFDRIQGATVYWLGTLVVC
                     GLILLCAVLKPREYVAMAIFTLTLDVLLIFQLGYSVAMRSSGIADGMLVVAMGAAIIV
                     AASGWFLIKMAPFKHSDAPRTAIAAPVRDVDHDTKVTWPAAIVSGIGALIAAIPFYIF
                     LGMTFYWFLTVGPAVLALGLVMIGAALFGLRAARRLGFLQQFAFIALMTGLGLCAFGI
                     IETFDTARLRNVGLSLVLMAGLCGGVAILNRVGWISAILGACAAACIGGAIWSEIGMR
                     KLVEPVTAGSASAVALALVLAFAQVLWSQGQSKSAESAMLSLRRFLTGATALALAALM
                     GTAGPTFMSMAFSPVGGSNAIPELQTGWRILQGLSALCAAGGCALALKRFDAVGAPLA
                     LVVSAVTVAFAAFSPNLGPTVLLGSVLLTGGARGLAVLAAIAALWIAGTFYYWLGWPL
                     VNKAYLLAALGLALVAAALALSPQDDMAEQGAGTRGWLHPVLAAVLLLAGPLAASGIT
                     LAGIRDNENVIATGRKIYLRTAPRDPRSLVQGDFMALNFEGGRMSNPLSATEKELYAR
                     ADVDARGIAIPKAFSESPEKPGSGEIVFPLRWKGGRWIVVTDAWYFKEGTGQRFANAR
                     FGEFRLRGDGRMLLVGLADGDLKPLQ"
     gene            9551..11638
                     /locus_tag="PK812_05385"
     CDS             9551..11638
                     /locus_tag="PK812_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HRJ69024.1"
                     /translation="MKLHAKTTGLSACPHDCPSTCSLEVEVLDANRIGRVRGAKDQTY
                     TKGVICAKVARYAERIHHPERLLHPLKRSGPKGSGRFERITWEEALDITAAKLLEAER
                     AFGAESVWPYYYAGTMGLVMRDGINRLRHVKRYSGQYSTICVTPAWTGYIAGTGVLAG
                     ADPREMAKSDCVVIWGTNPVATQVNVMTHAVAARKERGAKIVVIDVYRTETMAQADLP
                     ILLRPGTDGALACAVMHVLFREGLADREYLDRYTDDPKGLERHLRRRTPEWAAAITGV
                     PVETIEAFARLVGTRPRSFFRLGYGFSRQRNGAVNMHAASCIAAVTGAWRHEGGGAFH
                     NNGAIFRWDKTRIEGLDAIDPGVRQLDQSQIGRVLTGDRKALKNGPPVKALFIQNTNP
                     VSIAPEQQKVKRGFARRDLFVCVHEQFMTETALMADIVLPATMFLEHDDIYQGGGHQH
                     IMLGPKLIEPPGECRSNHWVVCELARRLGAEHVGFAGEARDLVDGLLSASGHGRLSAL
                     EKTSWKDVQPDFETAHFLKGFAWPDGRFRFRPQWTSVPAKNDGPMGPWKRLPQFPDHF
                     PVTEKPDARHPFKLVTAPARSFLNSSFTETAGSMAREVRPNLMIHPADAARLGIGDGD
                     LLEVGNRRGSVFLHARLFDGVLPGVTIAESIFPNRAHHMGRGINTLTSADQIAPFGGA
                     ALHDTKVWIRRAQ"
     gene            11727..12065
                     /locus_tag="PK812_05390"
     CDS             11727..12065
                     /locus_tag="PK812_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69025.1"
                     /translation="MWNVVRNAVVAAGLGVVLVGCQGVPANYKPPVDLKNASVPKIQN
                     YLHDTCIRSQRAKQSLDTQALSKACQCYSQRAVKAADKADLDFLRDKGYFSDAARPKI
                     QTVFNACGLK"
     gene            complement(12175..12645)
                     /locus_tag="PK812_05395"
     CDS             complement(12175..12645)
                     /locus_tag="PK812_05395"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRJ69026.1"
                     /translation="MTEKIVVGSTAPDFTLSTDGGGSIALSKLRGRKVVLYFYPKDDT
                     SGCTMEAKDFNALATDFAKAGTDIVGVSPDTTASHDKFKKKYGLCFPLAADPDKAMLH
                     AYGVWVEKSMYGRKYMGVERTTVLVDAQGRIAHVWNKVKVPGHAAEVLKAAKAL"
     gene            12778..>13282
                     /locus_tag="PK812_05400"
     CDS             12778..>13282
                     /locus_tag="PK812_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69027.1"
                     /translation="MATGRTQDPAQPAVAHSAPAAKGIGKTSRRKWALWVASGIAGTL
                     TAILLGIALILFLGIEGNAMVARMVRERIVLALQERIDPSLRLAIGAVDLKRKESGTA
                     VRVSDFTVKGPDGRVLVSAPSGMVVVDTTSLFVFRLIPTEVVLDDLHVFVEISEAGTL
                     SISPGDGA"
BASE COUNT         2128 a         4305 c         4467 g         2382 t
ORIGIN      
        1 gggcatggcc tgcgatcgcc aggcttgtgt cggccggctg gcggatgggc gggccgtctc
       61 tgtggtgacc gatgccgccg cctttgcgga ggattgcgag cgcgctgcga tcatcgttac
      121 gccgctggtg gcgcctcatg cctgccgcga gcacgccgaa gtttacgaca gggactattt
      181 cgccacgttt ggcgcgacct atctctatgc gcggaatgcc gggttccggc acgaaaccgc
      241 ccgcaacgag cggctcgacc ggccatggtc gccaaagccc gtaacgcgcc agcccgacat
      301 tccgcaggcg ccgtccttcg atatgccgcg ccaaccctgg gacagtcagg ccccagacga
      361 aaccgttcag taacggcgga tcaggctgac gagcttgccc tggatgcgca ccttgtccgg
      421 accgaagatc cgggtttcat agatcggatt ggcggcttcc aatgcgatgg atgcaccgcg
      481 tttgcgcaga cgcttcagcg tcgcctcctc atcgtcgatg aggtaggcga caacgatatc
      541 tccggtgtcc gcagtctcct gcttcttgat caccacggtg tcgccgtcaa aaatgccggc
      601 ctcgatcatc gagtcaccgc gcacttcaag cgcgtagtgc tcgccgtgag ccaggaagtc
      661 cggcgagatg ttgatggtgt ggctgcggtt ctggatggcc gagatcggcg tgccggcggc
      721 aatgcgcccc atcaccggga tggcaatcga gcgcacctcg tccggatcgt aggtctgcgg
      781 ggcgctcaac tgcgccggcg cgcgcacctt gccgagatcg ccctcgatca cgctgggatt
      841 gaatcgctgg cggcccggcc gggcaacgcc gctggttgat tcgggcagcc gcagcacttc
      901 cagcgcgcgc gcccgattgg gcaggcggcg gatgaagcca cgctcttcca gggcaaggat
      961 caggcggtga atgcccgact tggagcgcag atcaagcgcc tctttcatct cgtcgaagga
     1021 cgggggaacg ccattctcgg tcaggcgttc gtggatgaaa cgcagcagct cgctctgctt
     1081 ccgggtcagc atgtcgatcg tcgccctttc gaatcgcctg tgcgctcaga gtcgccttca
     1141 atctccaaac aaagcatgaa caaactagca tgttcgccat gcgttcgcaa ggacgcctcg
     1201 ttctgtttca ggcggtcaac agcctcagcg ggatcatgcg gcaggtctcg cccgcctttg
     1261 ctgccggcgc gaagggcgcg cggatcgtca gggcctgcga ggccgcgagc accgccagca
     1321 tcgaggagtc ctggaccgga tggggctcca cgaccggcag gccgcgctgg cggccggtga
     1381 tgcgggagcg cacgtaatcc tgcctgaggt cgttctcctt catgtcggcg ccgaggatcg
     1441 ccgcctcgga aggatcgatc ccggcaccgg ggtcgccgag cagggcgcgg atcagaggcc
     1501 gtatgaagac aatcgtgcaa actattgatg atacaggatt tcccggcaaa ccaagaatgc
     1561 gcatcgagcc aagagagcca aaaatgagcg gctttcctgg gcgcatggcg atgcgccaga
     1621 agccgagcgt catgccatcc ttggccaatg ctgattgcac cagatcatgg tcgccaaccg
     1681 aggcgccgcc aatggtgacg agaacgtctg ccttgaggtc gcgggcgcgg ccgatcgccg
     1741 cttccagact ggcgaaatcg tctgtcgcga tgcccagcag gtgcgcctcg gccccgtagg
     1801 cctcggcggc ggcggcaatg gcgaaattgt tggaggcgac gatctggtcc tgtccggggt
     1861 tctcgccggg cagcaccagt tcgtcaccgg tcgccagaac ggcgacacgc ggcctgcgca
     1921 cgacgggcac aacggcatgg ttcatcgcgg cggccagcgc gacttcggtc ggacccagcc
     1981 gtgttcccgc cttcaggagc gtctggcctg ccgcgaagtc gaggccggcc gtgcgcacga
     2041 actgtcccgg cctgaccgac tcggccacgg tgatgcgcgc gccctcggcg ctcgcgttct
     2101 cctgaatgag tatggcatct gcgccgtccg ggatcggcgc tccggtaaag atgcgcacgg
     2161 tctggcctgg accgatggcg ccggcaaacg cctgcccggc agcgctctcg ccgatcaggg
     2221 tcaggaccgc tccggccggg gtatcggccg cgcgcacggc atagccatcc atcgcggaat
     2281 tcgcccaggg cggctgcgtg cgtcgcgctg cgaggtcgga ggcgagggtg cgtccgccgg
     2341 cggccgtcaa cgccaccgtc tcgctttcca gcgtgcgcac gccatccagc acgcgccgca
     2401 gcgcttcctc gaccggcaac aggggggcgc ccgccacgtc aggcctccgc cacccaatgg
     2461 ccggacttgc cgccgagctt ttccagcagg cggatgccgt cgatccgcat gccgcgatcg
     2521 gcggccttgg ccatgtcgta gattgtcagg caggccaccg agacggcggt cagcgcctcc
     2581 atttcaaccc cggtctgacc ggagacccgg gcgagaccgg tgacccggat cccgggcagg
     2641 gagtcatcca ggtcgaagtc gatcgatgcc ttggtgagca gcagcggatg gcagagcggg
     2701 atcaggtcgt gcgtcttttt cgccgccatg atgccggcga tgcgggcggt ggcgagcacg
     2761 tcaccctttt tcgcggcgcc ggcacggatc atcgccaacg tttccggctg catgatcacc
     2821 agaccctcgg cgacggcggt ccggctcgtg acggccttgt cgccgacatc gaccatgctc
     2881 gcgcgccctt gcggatcaat gtgcgtgagg ccactcatgc cgcgatcccc agcaggtccc
     2941 gagtcgccgc cgtcacatcc tgctgtcgca tcaggctctc tcccaccagg aaggtgcgaa
     3001 tgccgcaggc cgcgaggtcc tcgcaatcgg catgggtgaa aatgccgctt tcgccgacga
     3061 tgattcgatc ccccgggatg cgccgcgcca gcgaacgtga tgtttccagg gagacctcga
     3121 aggtccgcag attgcggttg ttgatgccga tcagcgggct gtcgagccgc agcgcccggt
     3181 caagctcctc ggaatcatgc acttcaatca gcacatccat gccgaggcct tgcgccgtcc
     3241 gcgtcaatgc ggcggcgacc gcgtcatcga cggcggccat gatcaccagg atgcaatcgg
     3301 cacccagcgc gcgggcttcg tacacctgat agggatcgta gaggaaatcc ttgcggatga
     3361 ccggcagggc gcaggccgcg cgcgccgcca cgagatattc gggggcgccc tggaaccagg
     3421 gggcgtcggt cagcaccgaa aggcaggccg cgccgccagc ctcataggcc cgcgcgagcg
     3481 ccggcggatc gaagtcggcg cggatcaatc ccttggaagg gcttgccttc ttgatttcgg
     3541 cgatcagcgc atggcgtccg gcggcatggg cactgcggat cgcatccaga aagccgcgga
     3601 ctgccggagc gttcgctgcg cggcgctcga tctcggcctg ggagacagcc gccttcgcgg
     3661 cggcgatctc ggcgcgcttg gtgatttcga tcttcgcgag aatgtcagac atgtccgttg
     3721 cttgccatag ccgcatgggc cggacaagcg ccatgggtag ttgacaagac aatcattgac
     3781 tagtcaattt tagggcatgg tgcagcgcgc tcgacccacg caggtttacg ctcttcccga
     3841 gccggagggc gggcggccgc tgcagttgtt cctgacctac cggctggcgg cgctgaacgc
     3901 ccggctgaac cggcaggcca gcgtggtcct gcggcgcagc ggcggcctca agattccgga
     3961 atggcgcgtg atcgcgttgc tggcgacgca tggcgtgatg aacgggcgcg gcatcggcgc
     4021 catagcaggg ctcgacgccg gactggtcag tcggactttc cgggcgctgg aacagcgcgg
     4081 ccttatcgcc ctgcgccgcg acgagggcga ccgccgcatc gcccatgcgg cgctgacaga
     4141 acacggacgc tccctgttcc tgtccgtgcg gccggtgatg gcggcgcgtc aggatcggtt
     4201 gctggcggcg ctgacggcgg acgagcgagc gctggttttc cgcatcatcg acaagctcgc
     4261 gatcgctgca gacgacgttt ccgtgcccga ggtgccgacg tgaaggcggc caacaccctt
     4321 gtcatcctgt cggacgagca tcaggcgcgc gcgctgggat gtgccggcca tcctttcgtc
     4381 cgcacgccca acctcgaccg gctggccgcc cggggcatgc ggttctccaa tgccgtcacg
     4441 ccaagcccga tctgcgtgcc ggcgcgcgcc gctttcgcga cgggtcagtt cgttcaccgt
     4501 tgcggctact gggacaattc catcggctat gacggccggg tgcggggctg gggccatgcg
     4561 ttgcaggagc atcgcattcc ggtcgagtcc attggcaagc tgcactacca gaaggaacgc
     4621 ctgccgaccg gcttcgatgc cgaacacatc ccgatgcacc tgtacaaggg cgttggcatg
     4681 gtctgggcct cgatccgcga tccgcttccg gagaaccgcc ccacggacaa gcgcatgctg
     4741 ggcgagtaca tcggtcccgg cgagtcggac tacacgcgct acgatcaggc ggtgacggat
     4801 ctggcgattg actggctgaa ggggcggggt tcggcgctgg aagccccctg gtgcctctat
     4861 gtcgggctgg tggcgccgca ctttccgctg gtcgcgcccc cggacttctt cgctctctat
     4921 ccgccagcga gtctgccgga acccaagctg ctgccgcgcc acggctacac gctgcactcc
     4981 tggttgcagg agcacgagga cttctggagc caggacagga cattgcgcga cgatgacgag
     5041 cgccgaaccg cgatggccgc ctactacggc ctcacgtcct ggctggacca caacgtgggg
     5101 cggctgatca gcgcgcttga gcaggccggt ctttccgaga agacgcgggt cctctacgcc
     5161 tcggaccacg gcgacaacct cggcgcgcgg ggccaatggg gcaagtcgaa cctctatcgc
     5221 gagtcggtcg acattccgct gatcatggct gggccggacg tggcgcaggg cgtatgtgcc
     5281 acgccggtca gtctgctgga tctctcggtc acgatcctgg actcgctcgg cctcgatccc
     5341 gccacggcgc tgccacaggc cgatgggcgc tcgctgttcg cgctggcgcg tgaaccggcc
     5401 gatgccgacc gcaccgtctt cagcgaatac catgcggtcg gctccaatac cgcagcctac
     5461 atgctgcgcc gcggccggtg gaagtaccac catttcgtgc ggcaccggcc ggaactgttt
     5521 gatctggcca ccgacccgga ggagacgcgg gacctcgccg gcacagcgga atgcgccgac
     5581 gtcctggcgg acatggagcg tcgcctgagg gcgatctgtg atccggaagc ggtggatcga
     5641 gccgccaagc gggaccaggt ggccctgatc gagcggctcg gcggcaagga agccgtattc
     5701 accatgggca aggcggttgc gggtggcacg ccgccgccaa aaacctgaga caacgccatt
     5761 cgacgggagg aaaccatgaa ccgcagaatc cttatggccg cgcttttggc cggcgccggt
     5821 ttcgccgccg ctcaaggggg tgccctggcg caatccaagc cctggccgca ggagaagccc
     5881 attcgctggc ttatcgggct tccgccggcc ggcacgacgg atccgttgac gcgggccgtg
     5941 gccgagcagc tttccaggag gctcggccag tccatcgtcg tcgagaacaa gcccggcgcc
     6001 aatcaggtca tcgccgcacg cgaaatggcg aatgcgccgg cggatggcta tacccttctc
     6061 agcatgggcg ggccgcaggt ctatctcgat gcgagcgtgc cggtcatcgg gcgcgggttc
     6121 gatccggtga tccggctcgt cacccagccc atgatcttgg ccggcacgac ggatcggccc
     6181 acgccggacc tgaagtcggt cctggccgca gccaaggcca atccgaagga ctgggctttc
     6241 gcgtcggccg ggcaggggtc ttcgcaccat gttgctggag aatatgtgaa ccggctggcg
     6301 ggcaccgcga tcacccatgt gccctaccgg ggcggcggac aggccatcca ggatgccgca
     6361 ggcggtcagg tgccgctgat catgatcggc gtcgggccca cgctggcgca catcaagtcg
     6421 ggaaagatgc ggggctacgc cgtcacgact gccagccgga tagcgagcct gccggatgtg
     6481 ccgacgctcc aggaacaggg ctttccggga gtcgatctgg cccagtggca cggtgtggcg
     6541 atcaagtccg ggacgcccgc ggagatcacg caacggctca atcgcgagat acgtgagatc
     6601 ttggcgacac ccgaactgcg cgcgctcatc gagcggcttg ccgccgtgcc cggagagggg
     6661 acgccggagg catggggggc cttctacaag gacgattttg cgaagtggac gcggctgatg
     6721 caggaactga agatcgaggc cgaataggcg ctccttcctt tttccacatt tcttcgcgac
     6781 tctgccattc tggggctcgc agcgtatcgg ctgcaacgga aggtcgtgcc gcatgaacgc
     6841 cccgggccaa ctcgaagcgc atctcccgcg cttccatcgg atcgtgcaat tgtccggagc
     6901 cgccttgctc ggcggcgggt tgatgctctg gatcgccgcg aactgggatt ctatcggcaa
     6961 actgcagaag ttctggctgg tcggcggcat tctcctggcg ttcggcgcga tcggcgcgac
     7021 gctcgcggcc tggcgaacgc ccgccgcgct cggcgccttt cttgccatcg gcgcgctgtt
     7081 cgccctgttc ggccaaacct accagaccgg ggccgatccc tggcagctct tcgcgctctg
     7141 ggcggcgctc ggcctgccgc tggccattgc cgcccgccat gatgcggtgt tcgtgccctg
     7201 gatggtggtg gcgttcgctg ccctgcacct gttcgtcgcc tccaactccg ccttttttgc
     7261 acatccgacc cttgggcttc tgctgccgat ctggtccggc ggcttcgcca tcgtggcggt
     7321 gctatatggg ttcaaccggt cgccatgggg agaaaacagc gcccgctggg ctgtccagct
     7381 cggcgcgctg gtgctggcaa gcctgatcgg cggtgtggcc ggcgccgcaa tcttcgatcg
     7441 catccagggg gcgactgtct actggctggg cacgctggtt gtttgcgggc ttatccttct
     7501 ttgcgccgtg ctcaagccgc gcgaatacgt ggcgatggcg atcttcacgc tgaccctcga
     7561 cgtgctgctg atcttccagc tcggctattc cgtcgcgatg cggtcgagcg gtattgccga
     7621 tggcatgctg gtggtcgcca tgggtgccgc gatcattgtc gcggcgagtg gctggttcct
     7681 gatcaagatg gcgcccttca agcattccga tgcgccgcga acggcgatcg ccgcgccggt
     7741 tcgggacgtc gaccacgaca cgaaggtgac atggccggca gccatcgtca gcggcatcgg
     7801 cgcgctgatc gcagccatac cgttctacat cttccttggc atgacattct attggttcct
     7861 cacggtggga ccggcggtgc tggcgctggg cctggtcatg attggcgcgg cgctcttcgg
     7921 tctgcgcgcg gccaggaggc tcggtttcct gcaacaattc gcattcatcg ccctgatgac
     7981 ggggctgggc ctctgcgctt tcggcatcat cgagaccttc gacaccgcca ggctgaggaa
     8041 tgtcgggcta tcgctggtgt tgatggccgg cctgtgcggt ggcgtggcga tcctgaaccg
     8101 tgtcggctgg atcagcgcca tcctgggggc ttgcgcggca gcctgcatcg gcggcgccat
     8161 ctggtccgag atcggcatgc gcaagctggt cgagccggtg acggctggct cggcctccgc
     8221 cgtggcgctg gcgctcgtgc tggcgttcgc gcaggtcctg tggagccagg gacagtcgaa
     8281 gagcgctgaa agcgccatgc tgtcgctgcg gcgcttcctg accggcgcga cggcgctggc
     8341 gctggcggcg ctgatgggga ccgccgggcc caccttcatg agcatggcgt tctcgcccgt
     8401 gggcggctcg aacgcgattc ccgaacttca gacgggttgg cgtatacttc agggcctatc
     8461 ggccctctgc gcggccggcg ggtgcgcgct ggcgctgaag cggttcgatg ccgtcggcgc
     8521 gccgctggcg ctggtcgtta gcgccgtcac ggtggccttt gcggcgtttt cgcccaatct
     8581 cgggccaacc gtgctgcttg gcagcgtgct gctgacgggc ggcgcgcggg ggctggccgt
     8641 tttggccgcc atcgccgcgc tctggattgc cggcaccttc tactactggc tcggctggcc
     8701 gctcgtgaac aaggcgtatt tgctcgcggc gctcggtctg gcgctcgtcg cggccgcgct
     8761 cgccctgtcg ccgcaggatg acatggcaga gcagggcgca ggcacccggg gctggctgca
     8821 tccggtgctc gcggcggtcc tgctgcttgc cggtccgctc gcggcttccg gcatcacgct
     8881 cgcgggcatc cgcgacaatg agaatgtcat cgccacgggc cggaagatct atctgcgtac
     8941 cgcaccgcgc gatccgcgat cgctggtgca gggcgacttc atggcgctca acttcgaagg
     9001 cggacgcatg agcaatccgc tgtccgcgac ggagaaagaa ctctacgccc gcgccgatgt
     9061 cgacgcgcgt ggcatcgcca tacccaaggc gttttcagaa tctccggaaa agccgggatc
     9121 gggggagatc gtgtttccgc tgcggtggaa gggcgggcgc tggatcgtgg tcaccgatgc
     9181 ctggtacttc aaggagggca ccggccagcg cttcgccaac gcccgcttcg gcgagttccg
     9241 gctgcgcggc gacgggcgca tgctgctggt cggcctggct gatggcgacc tgaagccgct
     9301 gcagtaggcc gaaagggcac ggctttcccg tgttttttcg gagatgcgcc cgggtttaga
     9361 cgattggatt cacactccgt tgtcgggcgc gcgctcaggg tgtggcgttt ccggcgcgcg
     9421 tccctgcggc cgggtcagag aaggccgcat tcttcccatg gtatttgtcc gccggattgg
     9481 gggcgggagg ggaacagggg tctgggcctt gcggcttgcc cgccgcgcgg gacggcggca
     9541 tgttgcgacc atgaaacttc atgccaagac cactggcctt tcggcctgcc cgcacgactg
     9601 cccctccacc tgctcgctgg aagtggaagt gcttgacgcc aatcgcatcg gtcgtgtgcg
     9661 cggggccaag gaccagacct acaccaaggg cgtgatctgc gcgaaggtcg cccgctacgc
     9721 cgagcggata caccatcccg agcggctgct tcatcccttg aagcgcagcg gtccgaaggg
     9781 ttcgggccgt ttcgagcgca ttacctggga agaggcgctg gacatcaccg ccgccaagtt
     9841 gctcgaggcg gagcgcgcgt tcggagcgga atcggtctgg ccctattact atgccggcac
     9901 aatgggcctg gtgatgcgcg acggcatcaa ccggctccgg cacgtcaagc gctactccgg
     9961 ccagtactcg accatctgcg tcacgccggc ctggactggc tatatcgctg gaaccggagt
    10021 tctggccggt gccgatccgc gcgagatggc caagtccgac tgcgtggtca tctggggcac
    10081 gaaccccgtg gcgacgcagg tcaacgtcat gacgcatgcc gtagcagccc ggaaggagcg
    10141 cggcgcgaag attgtcgtga tcgatgtcta ccgcacggaa accatggcgc aggcggactt
    10201 gccgatcctg ctacggcccg gcactgacgg ggcgctggcc tgcgccgtga tgcacgtgct
    10261 gttccgcgag gggctggcgg accgggagta tctcgaccgc tacaccgacg accccaaggg
    10321 gctggagcgg catctgcgcc gccgcacgcc ggaatgggcg gcggccatca cgggcgtgcc
    10381 ggtcgaaacg atcgaagcct tcgccaggct ggtcggcacg cgtccgcgca gtttcttccg
    10441 gctgggctac gggttttccc gccagcgcaa cggagcggtc aacatgcatg ccgccagttg
    10501 catagcggcg gtgaccggcg cctggcgcca cgaaggcggc ggcgcgttcc acaacaacgg
    10561 cgcgatcttc cgttgggaca agacgcgcat cgaagggctg gacgccatcg atcccggcgt
    10621 gcgccagctc gaccagtcgc agatcggccg cgttctgaca ggcgaccgga aggcgctgaa
    10681 gaacgggcca ccggtaaagg cgctgttcat ccagaacaca aatccggtgt ccattgcgcc
    10741 ggaacagcag aaagtgaagc gcggatttgc ccggcgcgac ctgttcgtct gcgtgcacga
    10801 gcagttcatg accgagacgg cactgatggc ggatatcgtg ctgccggcga ccatgttcct
    10861 ggaacatgac gacatctatc aggggggcgg acaccagcac atcatgctcg ggcccaagct
    10921 gatcgagccg ccgggcgagt gccgctcaaa tcactgggtg gtttgcgagc tggcgcgccg
    10981 cctcggcgcc gaacacgtcg gctttgccgg cgaggcccgc gatcttgtgg atggcttgct
    11041 ctctgccagc gggcatggcc ggctgtccgc gctggagaag acctcgtgga aggacgtgca
    11101 gccggatttc gagaccgcgc atttcctcaa ggggttcgcg tggccggacg gccgattccg
    11161 cttccggccg caatggacca gcgttcccgc caagaacgat ggtccgatgg gaccctggaa
    11221 gcggttgcca caatttcctg accacttccc ggtcaccgag aagccggatg cgcgccatcc
    11281 cttcaagctg gtcactgcgc cggcgcgttc gttcctcaat tcgagcttca cggaaaccgc
    11341 cggatcgatg gcgcgcgagg tgcggcccaa cctcatgatc caccctgccg atgccgcccg
    11401 gctcggcatc ggggacggcg acttgctcga agtcggcaac cgtcgcggct cggtgttcct
    11461 gcatgcgcgg ctgttcgatg gcgtgctgcc gggcgtgacg atcgccgagt cgatctttcc
    11521 caaccgggcg catcacatgg gacgcgggat caacacgctg accagcgccg atcagattgc
    11581 cccgttcgga ggggccgcgc tacacgacac aaaagtctgg attcgtcggg cacagtagcg
    11641 gccgtggcac ggtttctctc ttgacgacac gggctgaccg atgcgctatg cgcgacgggg
    11701 attctggctt agagggtgaa ttcgagatgt ggaatgtggt tcgcaacgcg gtcgtcgccg
    11761 cgggtctggg tgttgtgctg gtcggctgtc agggcgttcc cgccaactat aagccgccgg
    11821 tcgatctgaa gaatgcctcg gtgccgaaga ttcagaacta tctccacgat acctgcatac
    11881 gctcccagcg cgccaagcag agccttgaca cgcaggcgct gtcgaaggcc tgccagtgtt
    11941 actcgcagcg cgccgtcaag gctgcggaca aggcagacct cgatttcctg cgcgacaagg
    12001 gctatttcag cgacgccgcg cgtccgaaga tccagacggt gttcaacgcc tgcggcctaa
    12061 agtaggcccg gatctcgcag tctctccgac gacaggggaa agggaacgcg cggctgttca
    12121 acgcgttcca cctcttgttc caggccgagt tcaagctgga gtattgctga gcggtcacag
    12181 agccttggcc gccttcagaa cctcggcggc gtgaccggga accttgacct tgttccagac
    12241 atgggcgatg cgcccctgcg catccacgag caccgtcgtg cgctcgacac ccatgtactt
    12301 gcgcccgtac atcgatttct cgacccagac cccgtaggcg tgcagcatgg ccttgtcggg
    12361 atcggccgca aggggaaagc agaggccgta tttcttcttg aacttgtcgt ggctcgcggt
    12421 cgtgtcgggg gagacgccga cgatgtccgt cccggccttg gcgaagtccg tcgcgagggc
    12481 attgaagtcc ttcgcttcca tggtgcatcc ggaggtatca tcctttggat agaaatagag
    12541 tacgaccttc cgccccctga gcttggacag ggctatcgat ccgccgccat cggtcgagag
    12601 ggtaaaatcc ggcgcggtcg atccgacgac aatcttctcc gtcataacaa ttctgctccc
    12661 ttgccttgcg taggccccat ttcatgggtc tccagcatgc acatatggcg tagcctgcgg
    12721 atgaatccag tgcgatttcc atccgctttc gcgcgcgctg cgctataagg gaatcgcatg
    12781 gcgacaggcc gcacccaaga cccggcgcaa ccggccgtcg cgcactccgc gccggccgcc
    12841 aagggcattg ggaaaacgtc ccgccgcaag tgggcactgt gggtcgcctc cggcatcgcg
    12901 ggcacgctta cggccattct gctgggcatc gcgctgatcc ttttccttgg catcgagggc
    12961 aacgccatgg tcgcgcgcat ggtgcgcgag cggattgtct tggcgctgca ggagcggatc
    13021 gacccctcgc tgcgcctcgc catcggcgcc gtcgacctga agcggaagga gagtggcacc
    13081 gcggtccggg tctcggactt cacggtaaag gggccggatg gacgcgtact ggtctcggcg
    13141 ccatccggga tggtggtcgt cgatacgaca tcgctgttcg ttttccggct gatccccact
    13201 gaggtggtgc tggacgacct gcatgtgttc gtcgagattt ccgaggcggg aacgctgtca
    13261 atctcgccgg gcgacggcgc cg
//
LOCUS       DAOMXN010000045        13002 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_2624_length_13002_cov_10.550552, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000045 DAOMXN010000000
VERSION     DAOMXN010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 13002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13002
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2624_length_13002_cov_10.550552"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="PK812_05405"
     CDS             complement(<1..200)
                     /locus_tag="PK812_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase"
                     /protein_id="HRJ69028.1"
                     /translation="MHPTEIRNPDYFHKVVDCQWACPAHTPVPEYIRLIAHGNYSDAY
                     MINWKSNVFPGILGRTCDRPCKA"
     gene            453..1130
                     /locus_tag="PK812_05410"
     CDS             453..1130
                     /locus_tag="PK812_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007068552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HRJ69029.1"
                     /translation="MHLILILVAMLLAAPAQAQTPPTEAERNGYGGLHAAAVRGDVAE
                     IRRLIAAGANPNARDGAQRTPVHVAAYFSRIEAMQALVAGGGDINALEHRKYDVVTIA
                     SVKDDEATLKAALALNANARAITSPYDGTALIAAAHLGHDGVVRQLIRAGAPLDHVNN
                     LGWTALIEAVILGDGGRRHQETVRALVEAGARLDLADREGRTPLQHARSRGYADIARI
                     IESKSAR"
     gene            complement(1183..2019)
                     /locus_tag="PK812_05415"
     CDS             complement(1183..2019)
                     /locus_tag="PK812_05415"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HRJ69030.1"
                     /translation="MLKDLLGSTILTPVLADGIFDRTPKSTVYMMSNSEDFYFGGWGN
                     DVVHGSDNSDLIYGNDGVDSLFGNFGHDTLYGGWGNDLLYGGAGFDHLDGGQGDDILW
                     GENGDDYLSGGEGIDAVDGGGGNDRLYGGGGNDSMYGGEGDDFVVGDAGNDWMEGQQG
                     RDYLVGSAGNDTIDGGAGNDTLSGGWGNDVLRGGEGIDRISLWDGDIRGGDHDIIADF
                     RNADGTFDRLTIDAFYRGATAFTQNGGDVDLTIATWDGGTYTLTFMGLSEAEVRSNVQ
                     FF"
     gene            complement(2179..2919)
                     /locus_tag="PK812_05420"
     CDS             complement(2179..2919)
                     /locus_tag="PK812_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HRJ69031.1"
                     /translation="MTYCVGVLVQDGLLMVADTRTNAGLDNIATFRKLHLFEQPGQKM
                     IALATAGNLGVTQAVISLLREGFRNPDTDVVETLLDQPSMFRAAQFVGHAIRAVGATD
                     GAALERSGASFDVTMLLGGQFGEARARLFMLYKAGNFIEATSDTPYLQIGEHKYGKPI
                     LDRAVTFRSDLYDALKLSLISMDSTMRSNLGVGLPIDLVVLRKDVLQWEVSRRIEAAE
                     PYFHDLRERWSAALRKAHNDIPPPPYRD"
     gene            complement(3013..3519)
                     /locus_tag="PK812_05425"
     CDS             complement(3013..3519)
                     /locus_tag="PK812_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69032.1"
                     /translation="MHRTRLVLGLGLLIAAAGTANANEHPMKPFFPGTDTCYARTYSA
                     AHLKSHPKQRVRVFQIVHPFAYASTDVGADFLMAVSFQLKGRKDRFGPMSTYCKGEPD
                     GTASCTVEGDMGRFKLSRGTGNALIVTINDLSDEGGPDLGKGGDDKVIVLHKAPMKAC
                     PKNTLDVD"
     gene            complement(3603..4427)
                     /locus_tag="PK812_05430"
     CDS             complement(3603..4427)
                     /locus_tag="PK812_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019905188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HRJ69033.1"
                     /translation="MRLNVRHETIYRYAAPATSVIQQLRLTPRGHEGQFVRGWRVEID
                     ADSRLDRSEDAYGNIMHVFSIDGPVHELSIMVEGDVETSDMNGVVSTGVERFPLQFWL
                     RETPLTRPDPAIVEFARDISGGEGGETLPALHAINAALYSGMRFSPGDTHAGTTATQA
                     FKAGSGVCQDFAHVFISAARALSIPARYVGGYYLRTDRDEQDAGHAWAEAHVAGVGWI
                     GFDPAHGVCATDRYVRVTAAPDYLEAAPIRGARIGGGQETLAVSVHVSQGPVILEE"
     gene            complement(4435..5376)
                     /locus_tag="PK812_05435"
     CDS             complement(4435..5376)
                     /locus_tag="PK812_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HRJ69034.1"
                     /translation="MLSRTADNLFWLSRYIERAENLARILDVASRMATLPSAYAGSSN
                     EWESAIATAACTDLFHSKYGTATRENVIEFLIASPDNPSSIRQCIETARSNARAVRTA
                     MTAEMWETINDAWLHIQKLDLRKLDAARLPNFLNFVKEASLRFDGAAYRTMLRTDHYW
                     FQRLGVFIERADNTARLLDVKYHVLLPEKEPIGGGLDYFQWSSILRAVSALTSFSWVY
                     RETVKPWLVADFLILSRKQPRSLVSTYAEINRVLDQLSEGYGRRGPSQRLARHTFGKL
                     QNAEIQAIFQGGLHEFLTEFTAENNALGQAINEQYLQ"
     gene            complement(5393..6814)
                     /locus_tag="PK812_05440"
     CDS             complement(5393..6814)
                     /locus_tag="PK812_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HRJ69035.1"
                     /translation="MAEKHFFDEMNGDDSVVREGYSTLAEWLKQAPADLLANRRIEAE
                     HFFRRIGITFNVYGDTQGTERLIPFDLIPRILTSAEWARLSRGLEQRVNALNAFIGDV
                     YGAREVVKAGLVPEDLVLNNPSFRPEMTRIKPPGGVYVHIAGIDVVRIDADTFYVLED
                     NARTPSGVSYMLENREAMLRLFPELFAAMRIAPVDNYTDNLLSALRSVAPATASGEPT
                     VVLLTPGQYNSAYYEHSFLADKMGIELVEGRDLFVRANVVYMRTTEGPRRVDVIYRRV
                     DDEFLDPLAFDPSSVLGVPGLMSAYKAGNVTIANAVGAGVSDDKAIYSYMPEIVRFFT
                     GTDPILQNVPTWRCREPEALKYVLANLHELVVKEVHGSGGYGMLVGPHATRAERDAFA
                     AKIKADPAGFIAQPTLALSTVPCFVDSGLAPRHVDLRPYVLSGKDGVRIVPGGLTRVA
                     LKKGSLVVNSSQGGGTKDTWVVE"
     gene            complement(6931..7497)
                     /gene="efp"
                     /locus_tag="PK812_05445"
     CDS             complement(6931..7497)
                     /gene="efp"
                     /locus_tag="PK812_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HRJ69036.1"
                     /translation="MRIIASNVRKGNILEHEGQLYAVLSAESFFPGKGTPTTQIDMRR
                     ISDGVKIALRYKTTEQVERAFVEDKEYSFLYKDGDGYHFMNQENYEQLQVSDDVIGDQ
                     AAFLQENMICILSVFEGRAVAIELPARVTLEIVETEPVTKGQTASSSYKPAILSNGVR
                     VMVPPHITAGTRVVVQTADGAYVERAKD"
     gene            7600..8631
                     /gene="epmA"
                     /locus_tag="PK812_05450"
     CDS             7600..8631
                     /gene="epmA"
                     /locus_tag="PK812_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012710079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HRJ69037.1"
                     /translation="MIAPSPWWMPDRHADRRPFLIARNRIAAAVRAWFESRDFVEATP
                     SGLQVSPGNEAHLHAFSTELRDVGGAATQRYLHTSPEFSCKKLLAAGEARLFTLGPVW
                     RNREGGPLHAPEFTMLEWYRAREDYGAIMEDCAALLRLAAETTETTKFSWKGRVCDAF
                     STPERLTLQEAFARHAGTDLRAADRDSLAGTARRLGLHQAQDDTWSDLFSRILGAVIE
                     PHLGFERPTILYEYPASEAAMARRKPDDPAVSERFELYVCGVELANCFGELTDPVEQR
                     ARFETEMLVKQEIFGEKYPIDEAFIAALALMPPASGGALGFDRLVMLATGATHIDQVQ
                     WAPVARGEA"
     gene            8628..9707
                     /locus_tag="PK812_05455"
     CDS             8628..9707
                     /locus_tag="PK812_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018448309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-2,3-aminomutase-like protein"
                     /protein_id="HRJ69038.1"
                     /translation="MTRPLRSIDDLIAAGLADADDRVDLHGVAARYAVAVTPAMAALI
                     DTAAPDDPIALQFVPRAQELIRSGEETDDPIADHPHAPVKGIVHRYADRVLLKATHTC
                     PVYCRFCFRREMVGPDGDGTLTRAEIDAALAYVAARPAIREVIVTGGDPFVLSERRIA
                     AITAAIAALPHVETIRWHSRVPVVEPERVTSAFVDALTRPAGRTRVTVAIHANHAREF
                     SPEAQAAVARLAEAGITLLSQSVLLRGVNDRADVLADLVRAFHANRIRPYYLHHGDLA
                     PGTGHFRLPLAEGLKLVEALRSALAGMTLPTYVLDLPGGHGKVPVARALPLADGRFLF
                     TDHSGRGHVYPPRWPETEKAAAVSG"
     gene            complement(9767..10015)
                     /locus_tag="PK812_05460"
     CDS             complement(9767..10015)
                     /locus_tag="PK812_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007198242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HRJ69039.1"
                     /translation="MTIDAIKATLLPEGKSFYDMGMMYATGRDCELDLVAAHCWFNIA
                     AAKGDRTAAERRAELAAEMSAMDIANAQRAAREWLSIH"
     gene            10418..10840
                     /locus_tag="PK812_05465"
     CDS             10418..10840
                     /locus_tag="PK812_05465"
                     /inference="COORDINATES: protein motif:HMM:NF021438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HRJ69040.1"
                     /translation="MPAPLRLATLAIPGLLWLASPALANDATGDWRTEKGRVRVADCG
                     GGLCATVTGINQPNDPRTGKPQTDVNNPNEKLRSRPILGISVLNGMKPAGDRVWKGSI
                     YNPEDGKTYAATMKLEGNRLMVSGCVASVLCKTQTWTR"
     gene            complement(10916..11611)
                     /locus_tag="PK812_05470"
     CDS             complement(10916..11611)
                     /locus_tag="PK812_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003527316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HRJ69041.1"
                     /translation="MAPIDLYYWPTPNGWKISIMLEECGLPYRLQFVDISKGEQFRPE
                     FLAISPNNRMPAIVDPDGPGGAPISVFESGAILQYLGRKTGLFYPAHERARVEVEQWL
                     MWQMGGLGPMCGQTHHFRHYAPEQVPYAIDRYTREVTRLYGVMNKRLSDRPYLAGDYS
                     IADIACIGWVRPWERQGQNLDDFPHLKRWFEEVMARPAVKRGMAAGEERRNAVNMKDP
                     QVQATLFGNKAKP"
     gene            11819..12370
                     /locus_tag="PK812_05475"
     CDS             11819..12370
                     /locus_tag="PK812_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HRJ69042.1"
                     /translation="METPAFVTYVVDFVRVHQAWAAPIAFALAFLESLAFLSLLVPST
                     AILIGISGLIGAAGVDFWPVWIAGGAGGVIGYSISYWLGVWFKEPIFRLWPFSRHPEL
                     VVRSHAFFERWGALSVFFGHFIGPVRAVIPVIAGSCGLNHLKFEIANVASAFLWITFV
                     LAPGYFLTASEIFSSFFPTLGLR"
     gene            12817..12945
                     /locus_tag="PK812_05480"
     CDS             12817..12945
                     /locus_tag="PK812_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69043.1"
                     /translation="MAAARAVVARAVVAPSVAAVAVVALAVVAPSVVAVAAVAAGA"
BASE COUNT         2224 a         4154 c         4201 g         2423 t
ORIGIN      
        1 gccttgcaag gccgatcgca cgtgcgcccg aggatgccgg ggaacacgtt ggacttccaa
       61 ttgatcatgt aggcgtcgga atagttcccg tgcgcgatca accggatgta ttcgggaacc
      121 ggcgtatgcg ccggacaggc ccactggcaa tccacgacct tgtgaaaata gtcgggattg
      181 cggatttccg tcggatgcaa cgcattcctc ctcgccgccg cacggcatcg gcagtgcttg
      241 tttttccggc agtctgatga ctgacaacgg attccgtcaa gtcagcattt gcgcgcacgc
      301 catcactttg atgcaggtca ccgtcacaaa aaacattcgt atattcatat atttaaatgg
      361 taggctggct tcagcacctt caaaatacat cgcgattgat ccgcccggcg tcccgtgcgc
      421 accatcggcg gatcaacccc gtggagagcg ccatgcatct gatcctgatc cttgtcgcca
      481 tgctgctggc ggcgcctgcc caggcgcaga cgcctcccac cgaggcggaa cgcaacggct
      541 atggcggact gcacgccgcg gcggtgcgcg gcgatgtcgc ggagatcagg cgcctcatcg
      601 ccgctggcgc caatccgaat gcccgcgatg gcgcgcagag aacgccggtc catgtcgcgg
      661 cctacttctc gcggatcgag gcgatgcagg cgctggtcgc cggcggcggc gacatcaatg
      721 ccctggaaca tcgcaagtat gacgtcgtaa ccattgcctc ggtgaaagac gacgaggcca
      781 cgctgaaggc tgcgctggcg ctgaacgcca atgcccgggc catcaccagt ccttatgacg
      841 gcacggccct gatcgccgcg gcgcatctcg gccacgacgg cgtggtgcgg cagctgatcc
      901 gggccggcgc gccgctcgac catgtcaaca acctcggctg gaccgcgttg atcgaggcag
      961 tgatccttgg ggatggcggc agacgccatc aggaaaccgt gcgggcgctg gttgaggcgg
     1021 gcgcccggct ggatctggcc gacagagaag gccgcacccc gctccagcat gcccgctcgc
     1081 gcggatatgc ggatatcgcc cgcatcatcg agagcaagtc cgcacgctga acgcacgcac
     1141 cctcccccgt tgccgggaga gggcgcgatc tgagtttctg cttcagaaga actggacgtt
     1201 gctgcgcact tccgcttcgc tcagtcccat gaaggtcagc gtgtaggtgc cgccatccca
     1261 ggttgcgatg gtcaggtcga cgtcgccgcc gttctgagtg aatgccgtcg cgccccgata
     1321 gaacgcatcg atcgtcagcc gatcgaaggt tccgtcggca ttgcggaagt cggcgatgat
     1381 gtcgtggtcg ccgccccgga tgtcgccgtc ccagaggctg atgcggtcga tgccttcgcc
     1441 accgcgcagc acgtcattgc cccatccccc ggaaagggtg tcgttgcccg cgccgccgtc
     1501 gatcgtgtca ttgccggcgc tgccgaccag atagtcgcga ccctgctggc cctccatcca
     1561 gtcgttgccg gcatcgccga ccacgaagtc gtcgccttcg ccgccgtaca tcgagtcatt
     1621 gccgccaccg ccgtagagcc ggtcgttgcc gccaccgcca tcgactgcat cgattccctc
     1681 gccaccggag aggtagtcgt cgccgttctc gccccacagg atgtcatcgc cctgaccgcc
     1741 gtcgaggtga tcaaaaccgg cgccgccata gaggaggtca ttgccccagc caccatacag
     1801 cgtgtcgtgg ccgaagttgc cgaacaggga atcaacgcca tcgttcccgt agatcagatc
     1861 gctgttgtca ctgccgtgga ccacatcgtt gccccagccg ccgaaataga agtcctcgct
     1921 gttcgacatc atgtagaccg tcgactttgg tgttctgtcg aagatgccat cagcaagaac
     1981 cggggtcagg atcgtagatc caagaaggtc tttcaacatg tccgtcactc ccaagttttc
     2041 atccggccaa tccggacttt caacgtgggg tacttttagc aattgcgctc atgatcagca
     2101 gtgtcttggc gcaccttctg aaatataaac tcagaattac tgaaattcat aaatttttat
     2161 tcttctggga gacgcgtctc aatcgcgata gggcggtggc ggaatgtcgt tgtgggcctt
     2221 gcgcaaggcc gcggaccagc gttcccgcag gtcatggaag tacggttccg ccgcctcgat
     2281 gcgacggctg acttcccact gcaagacatc cttgcggaga acgacaagat cgatcggcag
     2341 accgacgcca agatttgagc gcatggtcga atccattgag atcagcgaca gcttgagcgc
     2401 gtcgtagagg tcgctgcgga acgtcacggc gcggtcgagg atcggcttgc cgtacttgtg
     2461 ctcgccgatc tgcaagtacg gcgtgtcact ggtcgcctcg atgaagttgc cggccttgta
     2521 gagcatgaac aggcgcgcgc gcgcttcgcc gaactggccg ccgagcagca tcgtcacgtc
     2581 gaacgaggcg ccggagcgtt ccagcgcggc gccgtccgtc gcgccgaccg cgcggatggc
     2641 atggccgacg aactgggcgg cgcggaacat cgagggctga tcgagcagcg tttcgaccac
     2701 gtccgtgtca gggttgcgga agccttcgcg cagcagcgag atcaccgcct gcgtcacgcc
     2761 gagattgccg gcggtcgcaa gcgcgatcat cttctggccg ggctgttcga acaggtgcag
     2821 cttgcggaag gtcgcaatgt tgtcgagtcc ggcgttggtg cgcgtgtccg ccaccatcag
     2881 caaaccgtcc tgaaccagca cgcccacgca ataggtcatc gcccctgccc ccgctcattc
     2941 acggagcgcg gattagcgct cccggcggca ggatgcaatc ggtgggccgg gcggcatgag
     3001 cgcgcccgat gcctagtcca cgtcgagcgt gttcttcgga caggccttca tcggcgcctt
     3061 gtgcaggacg atgaccttgt cgtcgccgcc cttgccgaga tcgggaccgc cttcgtcgct
     3121 caggtcgttg atcgtgacaa tcagcgcatt tccggtgccg cggctcaatt tgaagcgtcc
     3181 catgtcgccc tcgaccgtac agctggccgt gccgtccggc tcgcctttgc agtaggtcga
     3241 catcgggcca aacctgtctt tcctgccctt gagctgaaag cttaccgcca tcaggaaatc
     3301 cgcgccgaca tccgtgcttg cgtaggcgaa gggatggacg atctggaaga cgcgcacgcg
     3361 ttgcttgggg tggctcttga ggtgcgccgc cgaataggtc cgggcgtaac aggtgtccgt
     3421 gccggggaag aatggcttca tcgggtgttc gttcgcgttc gccgttccgg cagcggcgat
     3481 gagcaggccg aggccgagga caagacgggt gcgatgcatg gcggttcctc ctgaacgtgc
     3541 cgccatcatt ggcaacttgc ctcggcgcgc cgtgcgaatg cgcacacccg gacgatacta
     3601 tctcactcct cgaggatcac cggcccctgg ctgacatgga cggatacggc cagagtctcc
     3661 tgtccgccgc cgatgcgcgc gccacggatc ggcgcggctt caaggtagtc cggcgcggcg
     3721 gtcacccgca cgtagcgatc cgttgcgcag acgccgtgcg ccggatcgaa accgatccag
     3781 ccgacgcccg ccacatgggc ttcggcccag gcatggccgg catcctgctc gtcacggtcg
     3841 gtgcgcagat aatagccccc gacatagcgg gccgggatac tcagggcgcg cgcggcggaa
     3901 atgaagacat gcgcgaaatc ctggcacacg ccggagccgg ccttgaacgc ctgcgtggcg
     3961 gtcgtgccgg catgggtgtc cccgggcgag aaccgcatcc cgctatagag cgccgcgttg
     4021 atggcgtgga gcgcaggcag ggtttcgccg ccctcgccgc ccgagatgtc gcgggcaaac
     4081 tcgacgatcg ccggatccgg ccgggtcagc ggcgtctcgc gcagccagaa ttgcagcgga
     4141 aaccgctcga cgccggtgct gaccacgcca ttcatgtcac tggtctcgac gtcgccttcc
     4201 accatgatgg acagttcgtg caccggcccg tcgatggaga agacgtgcat gatgttgccg
     4261 taagcgtctt ccgagcggtc aaggcggctg tcggcgtcga tctcgacgcg ccagccgcgg
     4321 acgaactggc cttcgtgccc ccggggcgtc aggcgcagct gctggatcac cgatgttgcc
     4381 ggcgcggcgt agcggtagat cgtctcgtgg cggacgttca ggcgcatgag gcgatcattg
     4441 caggtattgt tcgttgatgg cctggccgag ggcgttgttc tcggcggtga actccgtcag
     4501 gaactcgtgc agtccgccct ggaaaatcgc ctggatctcg gcgttctgga gcttgccgaa
     4561 ggtatggcgc gccagacgct gcgagggtcc gcgccggccg tagccctccg agagctggtc
     4621 gagaacccgg tttatctcgg cataggtgga aacaagcgag cgcggctgct tgcggctcag
     4681 gatgaggaag tcggcgacca gccaaggctt gaccgtttcc cgatagaccc agctgaacga
     4741 cgtcagcgcc gaaaccgcgc gcaggatcga cgaccactgg aaatagtcga gaccgccgcc
     4801 aatcggctcc ttttccggca acagcacgtg gtatttcacg tccagcaggc gcgcggtgtt
     4861 gtcggcgcgc tcgatgaaca cgccgagccg ctggaaccag tagtggtccg tgcgcagcat
     4921 ggtgcgatag gccgctccgt cgaaccgcag ggaggcttcc ttcacgaagt tcaggaagtt
     4981 cggcaggcgc gcggcgtcaa gcttgcgcag atcgagcttc tggatgtgaa gccaggcatc
     5041 gttgattgtc tcccacatct ccgccgtcat ggcggtgcgc accgcccggg cattggagcg
     5101 cgcggtctcg atgcactggc ggatcgacga gggattgtcc ggcgaggcga tgaggaactc
     5161 gatgacgttc tcgcgcgtcg ccgtgccgta cttgctgtga aacaggtccg tgcaggcggc
     5221 cgtggcgatg gcgctttccc attcgttgga cgagccggca taggccgagg gcagcgtcgc
     5281 catgcgggag gcgacatcga ggatacgcgc gaggttctcg gcccgctcga tgtagcgaga
     5341 gagccagaac aggttgtctg cggtgcggct cagcattcgt tcaactcccg ccctactcga
     5401 cgacccaggt gtccttggtg cccccgccct gcgacgagtt caccacaagc gaccccttct
     5461 tgagcgcgac gcgcgtcaag ccgcccggca cgatgcgcac gccatccttg cccgacagaa
     5521 catagggacg cagatcaacg tggcgcggcg cgagaccgct gtcgacgaaa caggggacgg
     5581 tcgagagcgc cagcgtcggc tgggcaatga agccggcggg atcggctttg atcttcgccg
     5641 cgaaggcatc gcgttcggcc cgggtcgcgt gcgggccgac cagcatgccg tagccgcccg
     5701 agccatgcac ctccttgacg accagttcgt gcaggttggc caacacgtac ttcagtgcct
     5761 ccggctcgcg gcaccgccag gtcgggacat tctgcaggat cggatcggtg ccggtgaaaa
     5821 acctcacgat ttccggcatg taggaataaa tcgccttgtc gtccgacacg ccggcgccga
     5881 ccgcgttggc gattgtcacg ttgccggcct tgtaggccga catcaggccg ggaacaccga
     5941 gaaccgagga tggatcgaag gcgagaggat cgaggaactc gtcatcgacc cggcggtaga
     6001 tcacgtcgac gcgccgcggc ccttccgtcg tgcgcatgta gacgacgttc gcccggacga
     6061 agagatcgcg gccctcgacc agttcgatgc ccatcttgtc ggcaaggaac gaatgctcat
     6121 agtaggccga attgtactgg cccggtgtca gcaggaccac cgtcggctcg cctgacgcgg
     6181 tggcgggcgc aaccgagcgc aaggccgaca gcagattgtc cgtgtaattg tcgacgggcg
     6241 cgatgcgcat cgccgcgaag agttcgggaa acaggcggag catcgcctcc cggttttcca
     6301 gcatgtagga gacgccggag ggcgtgcgcg cattgtcttc cagcacgtag aacgtatcgg
     6361 catcgatgcg cacgacatca atgcccgcga tgtgcacata aacgccaccc ggcggcttga
     6421 tgcgcgtcat ttccgggcgg aatgatggat tattgagcac caggtcttcc ggcacgagcc
     6481 cggccttgac cacctcgcgc gcaccataga catcgccgat gaaggcgttg agggcattga
     6541 cacgctgttc aagccccctg gacagccgcg cccattccgc cgaggtcagg atgcgcggga
     6601 tcagatcgaa cgggatcagc cgttccgtgc cctgcgtgtc gccatagaca ttgaacgtga
     6661 tgccgatacg ccggaaaaag tgctcggcct cgatacggcg gttggcgagc aggtccgccg
     6721 gagcctgctt cagccactcc gcgagcgtgc tatagccctc gcgcacgact gagtcgtcgc
     6781 cattcatctc gtcgaagaaa tgcttttccg ccatgcgccc ccgcgaactc cccggctctc
     6841 aggcaagcct attaggaagc gctatgcaag ggggatgccg gaaccgggcc ggacgcgggc
     6901 gtgccgctcc ctcagtgcgg cgcgccagcc tcagtccttg gcgcgttcga cataggcacc
     6961 gtcggcggtc tgcaccacca cgcgcgtgcc ggcggtgata tgcggcggca ccatcacgcg
     7021 tacgccgttc gagagaattg ccggcttgta ggaggacgac gcggtctggc ccttggtgac
     7081 gggctcagtc tcgacaatct ccagcgtcac gcgcgccgga agctcgatcg cgacggcgcg
     7141 accctcgaaa accgagagga tgcagatcat gttctcctgg aggaaggcgg cctgatcgcc
     7201 gatcacgtca tcggaaacct ggagctgctc gtagttctcc tggttcatga aatgatagcc
     7261 gtcgccatcc ttgtagagga acgagtattc cttatcctcg acaaaggcgc gctcgacctg
     7321 ctccgtcgtc ttgtagcgca gcgcgatctt cacgccgtcc gagatgcggc gcatgtcgat
     7381 ctgggtcgtc ggcgtgccct tgccgggaaa gaagctctcg gcggacagca cggcgtagag
     7441 ctgcccttcg tgttcgagga tgttgccttt gcggacgttg gaagcgataa ttctcaccgg
     7501 aatggtcctt cgaagcggtc gtgactggag ttggcggctc ctttgcccta atcggaccgg
     7561 tttgaaaagc ccgccggacc atattcgctt cgccacgcca tgatcgcccc ttccccctgg
     7621 tggatgccgg atcgccacgc cgaccggcgc cctttcctga ttgcccgcaa ccgtatcgcg
     7681 gcggcggtcc gggcctggtt cgagtcccgc gacttcgtcg aggcgacgcc gtccggattg
     7741 caggtttcgc ccggaaacga ggcgcatttg cacgcgtttt cgacggaact gcgggatgtg
     7801 ggcggggctg cgacccaacg ctacctgcac acgtcgccgg aattttcctg caagaagctg
     7861 ctggcggcgg gcgaagcgcg gctgttcacg ctcgggccgg tctggcgcaa ccgcgaaggt
     7921 ggaccgctgc acgcgccgga gttcacgatg ctggaatggt accgcgcgcg ggaagactac
     7981 ggggccatca tggaggactg cgccgcgctg ctgcgactgg cggccgaaac gaccgaaacg
     8041 acgaaatttt cgtggaaagg ccgcgtttgc gatgcgtttt cgacgcctga acggctgacg
     8101 ctgcaggaag cgttcgcgcg ccatgccggc accgacctgc gcgccgccga ccgcgacagt
     8161 cttgccggaa cggcccgccg tcttggcctt catcaggccc aggacgatac gtggagcgat
     8221 cttttcagcc ggattctggg cgcggtcatc gaaccgcacc tcggattcga gcggccgacg
     8281 atcctctacg aatatccggc cagcgaggcg gcgatggccc ggcgcaagcc cgatgatccg
     8341 gcggtgagtg aacggttcga gttgtatgtc tgcggcgtgg aactcgcgaa ctgcttcggc
     8401 gaactcacgg acccggttga gcagcgcgcc cggtttgaga ccgaaatgct cgtaaaacag
     8461 gaaatcttcg gagagaaata ccccatcgat gaggcgttca ttgccgcgct cgccctgatg
     8521 ccgccggcga gcggcggcgc gctcggattc gaccgcttgg tgatgctggc gacgggcgcg
     8581 acgcacatag accaggtgca atgggccccg gtggcgcggg gcgaggcatg acgcgaccgc
     8641 tgcgcagcat cgatgacctg atcgccgccg ggctcgctga cgccgatgac cgcgtggacc
     8701 tgcatggcgt ggcggcgcgc tatgccgtgg cggtgacgcc ggccatggca gctctcatcg
     8761 acacagccgc gcccgacgat cccatcgccc ttcagttcgt cccgcgagcg caggaactga
     8821 tacgctcggg ggaggaaacc gacgatccca tcgccgacca tccccatgcc ccggtcaagg
     8881 gcatcgtcca ccgttatgcc gaccgcgtgc tgctcaaggc cacgcacacg tgcccggtct
     8941 actgccgttt ctgtttccgg cgcgagatgg tcgggccgga tggcgacggc acgctcacgc
     9001 gggcggagat cgatgcggcg ctggcctatg tcgcagcgcg gccggcaatc cgcgaggtca
     9061 tcgtcaccgg cggcgacccg ttcgtcctgt ccgaacggcg catcgccgcg atcaccgccg
     9121 cgatcgccgc actcccgcat gtggaaacga tccgctggca cagccgcgtg ccggtcgttg
     9181 agccggagcg cgtgacctcg gccttcgtcg acgccctcac ccgtccggcc ggacgcacga
     9241 gggtgaccgt ggcgatccat gccaatcacg cgcgcgagtt ttctcccgag gcgcaagccg
     9301 ccgtggcgcg gctcgccgag gctggaatca ccctgctgag ccagtccgtg ctgctgcgcg
     9361 gcgtgaatga ccgggcggat gtgctggccg atctcgtcag ggcgtttcat gccaaccgca
     9421 tccggcccta ctacctgcat catggcgatc tggcgccggg cacgggccac ttccggctgc
     9481 ccctggccga ggggctgaag ctggtcgagg ccctgagaag cgcgcttgcc ggcatgactc
     9541 tgccgactta cgtgctggac ctgcccggcg gacacggcaa ggttccggtg gcccgggcgc
     9601 ttcccctggc tgacggccgg ttcttgttca ccgatcacag cgggcgaggg catgtctacc
     9661 cgccgcggtg gcccgaaacg gaaaaggcgg ccgccgtttc cggctgaccg cccctcctgc
     9721 tcctgatctg tctgacgccg ccccggatcc ggtggcggtg cgcgggtcag tgaatggaca
     9781 accactcacg ggccgcgcgc tgggcgttgg cgatgtccat cgccgacatt tccgccgcga
     9841 gttcggcgcg ccgctcggct gccgtacggt cacctttggc ggcggcgatg ttgaaccagc
     9901 aatgcgccgc cacgagatcg agttcgcagt cgcgcccggt ggcgtacatc atgcccatgt
     9961 cgtagaagga cttgccttcc ggaagcaacg tcgccttgat tgcatcgatc gtcatttcca
    10021 cacccgcctt atttgtttga attctgccct gttgaagtcg tttcgaatgc ttgttttgct
    10081 gcattcgttg aaagcgagat tgcgccggat tcttcaagag cgccttaaag tcgctggtga
    10141 acagggcctt tccgaaatcc gaacaaagcg taagtgtgca ggcccgttta cctttcttcc
    10201 cgggatttcc ctttgattac aaaggcatgt ggcgcgcgga taaagcctaa tgaactcgtg
    10261 cgttgagggc ggattcaccc tcttgcgccg aactgcgaaa aatgcggtct ttctgcccgc
    10321 cactttcttg gcgaattccc gacatagcga cggctgccgg agcttggcgc ccgcttcgcc
    10381 gggcgccgca ccgccagacg tcagaaggac ctgcatgatg ccagcaccgc ttcgccttgc
    10441 cacactcgcc attccgggac tgctttggct tgcatctccc gccctggcca acgacgcgac
    10501 gggcgactgg cgcacggaaa aggggcgcgt ccgcgtcgcc gattgcggcg gcgggctgtg
    10561 cgcgacggtc acggggatca accagccgaa cgatccgcgc accggcaagc cgcagaccga
    10621 cgtcaataac ccgaatgaaa agctccggtc ccgccccatc ctcgggattt cagtcctgaa
    10681 cggcatgaag cccgccggag accgcgtctg gaagggctcg atctacaatc cggaagacgg
    10741 caagacctat gccgccacga tgaagcttga gggcaatcgc ctcatggtct cgggctgcgt
    10801 cgccagcgtc ctgtgcaaga cgcagacctg gacgcgctag gtgggcgcgc atccagggtg
    10861 cggaggccgt tccagagcag agtgaaaccc gctggcgcga tccgcgcggg agcgctcacg
    10921 gtttcgcctt gttgccgaac agcgtcgcct gaacctgcgg gtccttcata ttgacggcgt
    10981 tgcgccgctc ctcgcccgcg gccatgccgc gcttgacggc aggccgggcc atgacctcct
    11041 cgaaccaccg cttgagatgc gggaaatcgt cgaggttctg gccctgccgc tcccagggcc
    11101 ggacccagcc gatgcaggca atgtcggcga tggaatagtc accggcaagg tagggccggt
    11161 ctgacagcct cttgttcatc acgccgtaga ggcgcgtgac ctcgcgggtg tagcggtcga
    11221 tggcataggg gacctgctcg ggtgcgtagt ggcggaaatg gtgggtctgc ccgcacatcg
    11281 gcccgagacc gcccatctgc cacatcagcc attgctcgac ttccacgcgc gcgcgctcgt
    11341 gcgcagggta gaaaagcccg gtcttgcgtc cgagatactg caggatcgcc cccgactcga
    11401 agacggagat cggtgccccg cccgggccgt cgggatcgac aatggcgggc atgcggttgt
    11461 tgggcgaaat cgccaggaac tccgggcgga actgctcgcc cttgctgatg tcgacgaact
    11521 gcagccgata cggcaggccg cactcctcga gcatgatgga aatcttccag ccgtttggcg
    11581 tgggccaata gtaaaggtcg atgggagcca tgggggtgcc tttcgcgtgt ttggcggcag
    11641 gctagcgcac ttgctgtcgg aattgaacgg ccggatcgcg cccccgatgg cttcggccgg
    11701 tcaactcttc atcaagtcag cgaaccgcgg tggcgatccg ttatggcgtt gttcacggcg
    11761 ccatggttga ctttccgaag aggcgccgca ggtctgctgc accaaccgtt ggagtaccat
    11821 ggaaacgccg gctttcgtca cctacgtggt ggacttcgtg cgcgtgcacc aggcctgggc
    11881 ggcgccgatc gcctttgccc tggcgttcct ggagtcgctc gccttcctgt cgctgctcgt
    11941 cccctcgact gcgatcctga tcggcatcag cggcctgatc ggtgcggcag gtgtcgactt
    12001 ctggccggtc tggatcgccg gaggcgcggg cggcgtgatc ggctactcca tctcctactg
    12061 gcttggcgtc tggttcaagg aaccgatctt tcgtctctgg cccttttcac gccaccccga
    12121 actcgtcgtc cgttcccatg cgttctttga acggtggggc gcattgagtg ttttttttgg
    12181 acatttcatc ggcccggttc gcgcggtgat cccggtaatc gccggaagtt gcggcctcaa
    12241 tcatctcaaa ttcgagatcg cgaacgtcgc atccgccttc ctctggatta cgtttgtgct
    12301 ggctcccggc tattttttga ccgcttcaga gatcttttcc agctttttcc cgaccctagg
    12361 actgcgctag cccttcgtgg ccacggcgga gttaaccctg tctcattttg ggccagtgta
    12421 aagaattttc atattgactt gcatccttaa gggttcgtaa aaaacttcca taccgttcga
    12481 tggaccatcg agcgcggtgg gtttggtttt ttgagtttgg aaccgcaggg agacaatcaa
    12541 tgacaaagtc ggttggcggc gcttcgggcg catgcagcac aggcggcagt tccggaagct
    12601 cgggatcgca cagcgcgtcc cgttctgcgg gcagcggctt cgccgcctcg gccagcaagg
    12661 cctgcggctc gaagggcgga tcgtcctcgt cgcatggggc ttccggcggc tcgtcgtcgc
    12721 actccagagg cgctgactgc gcgccgaagg gcgggtcatc cagccacagc agtggctccc
    12781 atggcgcctc ctccaagaag gactgcggcc cgaggggtgg cagcggctcg ggcggtggtg
    12841 gctcgggcgg tggtggctcc gtcggtggcg gcggtggcgg tggtggctct ggcggtggtg
    12901 gctccgtcgg tggtggcggt ggcggcggtg gcggcgggag cgtgacctgc cgtcctccgg
    12961 tccagccgcc ggtgtgtccg ccgatcggtc gtccgccggt cc
//
LOCUS       DAOMXN010000046        12895 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_2652_length_12895_cov_12.900078, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000046 DAOMXN010000000
VERSION     DAOMXN010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 12895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12895
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2652_length_12895_cov_12.900078"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1053
                     /locus_tag="PK812_05485"
     CDS             <1..1053
                     /locus_tag="PK812_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HRJ69044.1"
                     /translation="QAQDVEARLDATRRERSDTDGLPAELLARRRLLAGEIESAEGAR
                     KVAADARAEGETILLTFDRVAREALTALSNVREQRARSETQLEAAKGRLVDLTREIEE
                     TLGVSPSTLGEISGIARPEDLPAAQAAQARLDDLRRERERIGAVNLRADQELAEVVAT
                     RADLAREHDELGEAIRKLRRGIDSLNAEGRNRLVAAFEVVNGHFKTLFTRLFGGGSAE
                     LQLIESDDPLEAGLEIMAHPPGKKPQVLTLLSGGEQALTATALIFAVFLTNPSPVCVL
                     DEVDAPLDDSNVERLCDLLADMARETETRFVIITHNPITMARMDRLFGVTMAERGVSQ
                     LVSVDLEAAERLAEAS"
     gene            1308..1664
                     /locus_tag="PK812_05490"
     CDS             1308..1664
                     /locus_tag="PK812_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HRJ69045.1"
                     /translation="MDKDGTSPKAGEDALQARLDELTKALDAKRQGSEEAARSKSSGS
                     DTGIGKAMGAGFRVASELAAAILVGGFLGWQLDLWFGSTPWFLMIFLILGIVAGFWNV
                     YRLAAKPTGPMAGTDK"
     gene            1723..2487
                     /locus_tag="PK812_05495"
     CDS             1723..2487
                     /locus_tag="PK812_05495"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013169185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HRJ69046.1"
                     /translation="MAAGGAVIDPIHQFEIKKITNITVGGLDLSFTNASLFMVIALGV
                     ISLIMIWGTSARAIVPGRLQSIAEMLYEFIEDTVQGTMGKSGRKFLPLVFSLFMFVLV
                     CNMVGLIPGTFTVTSHIIVTFALAAVVILTVLIYGIMKHGTHFFGLFVPSGVPKPLLL
                     LLTPIEIISFVSRPISLSMRLFANMLAGHIALKVFAGFILMMTAAGGVAVALSPLPLL
                     MIVALTALEFLVAALQAFVFSVLTCIYLNDALHPGH"
     gene            2538..2765
                     /locus_tag="PK812_05500"
     CDS             2538..2765
                     /locus_tag="PK812_05500"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HRJ69047.1"
                     /translation="MDANAAKLIGAGLAAIGMGAAAIGVGTIFGNFLSGALRNPSAAD
                     GQFTRAFIGAALAEGLGIFAFVVALILLFVK"
     gene            2858..3400
                     /locus_tag="PK812_05505"
     CDS             2858..3400
                     /locus_tag="PK812_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008839272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B'"
                     /protein_id="HRJ69048.1"
                     /translation="MSLFITAAHAASGAGGGGAFPPFDASTFAGQLFWLAVVFGALYW
                     LMANVALPRVASIIEDRRARIAADLDTAATAQKAAADAQKAFEANLAQAKAKAQGIAQ
                     SARDAASREADARRHQVETDLTAKMVAAEKAIAETKAKAMANVEGIATDAAAAIVERL
                     GGSKPGDKAITDAVAALSQR"
     gene            3411..3881
                     /locus_tag="PK812_05510"
     CDS             3411..3881
                     /locus_tag="PK812_05510"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113999.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. Subunit B is part of the
                     membrane proton channel; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP F0F1 synthase subunit B"
                     /protein_id="HRJ69049.1"
                     /translation="MSDTLWVAVGFVIFLGIVAYYGGHKALLSGIDGRAQKIAAELAE
                     AKRLREEAEALMASFKAKQAQAEKDAEAMIAAAKAEAERLKVEAGQKLDDFVRRRTAQ
                     AELKIAQAEAQASADVRAAAADLAAAVAGKVLAGQGASDSAFSAALGEIKAKMN"
     gene            complement(3929..6292)
                     /locus_tag="PK812_05515"
     CDS             complement(3929..6292)
                     /locus_tag="PK812_05515"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HRJ69050.1"
                     /translation="MRIFQTLGAPTAHSNGFDLSLDEGWTLRVRAMSETLLRIVVAPP
                     EGLALDRTWMIAPEGDTPAEGRSRESLAGFSPVAADLTTSNGDHILTAGRFRVTLRGN
                     PVRMDVEQRVGDDWKPWLRDRATGGLALCDGDRRIRHFQMRLDNDRHFGLGDKTGPLD
                     RTGRRFRLLQLDALGYDAEIGDPLYKHTPYMAVQSGDVCGGLFYDTLAPMTFDLGCER
                     SNYHGIYRYVEAEERGLDLWLVAGPDLAATTRRFTMLTGRPAFPPRWSYGFAFTTMHH
                     ADDAEAQQVIAAFAERCRKEEIAISAIHFGSGYSSRGKRRYVFTWNRQKFPDPSALFA
                     KLKALGFYTVANLKPVLIDDHPDYASVKAAGGFVKARDGAPVLEQFWDGMGSYLDFTN
                     EQTAHWWQQRLVEQVLDIGFDAGWNDNNEYEIGQEDAIATGHGRPFPATAARPLHALL
                     MTRATFEASRARAPASRPYTITRAGPVGLHRYAETWTGDNATSWHTLKWNLRNGLSMA
                     LSGQSNVGHDIGGFTGPRPDGELLCRFIEMMALHPRAVMNSWKPHVAPDWTAATVPWL
                     YPEVLPQIRAALDLRTRFLPLIYTLAQRYHAKGEPMIRPLFHDFANDPDAYRDQDAMM
                     LGPDVLFSPVVEPGARVKTQYLPLGPSGWVDFHSGQVHAAGQNVTVAADLGCPAVFVR
                     VGAALVLASALPHVRPHDAPARQLYLSLAPGSGEGGGIHFEDDGTSWDFQAGNALVLS
                     IRLTWTADEARVRLTPSAGSAWNAGVASNWSVAGSGLKGRHISIEPA"
     gene            complement(6300..7397)
                     /gene="ugpC"
                     /locus_tag="PK812_05520"
     CDS             complement(6300..7397)
                     /gene="ugpC"
                     /locus_tag="PK812_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020011945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HRJ69051.1"
                     /translation="MATLDLRQVTKSFGATKVIHGVDLSIGHGEFIVFVGPSGCGKST
                     LLRLIAGLESVTGGAIEIDGALVNDVPAAERGLAMVFQSYALYPHMSVRQNLSFGLEN
                     LKTPKPEIQRRVAEAARMLRMEHLLDRRPGQLSGGQRQRVAIGRAVVREPKIFLFDEP
                     LSNLDAELRVQMRIEIGNLHQRLGNTMIYVTHDQVEAMTMADRIAVLKDGRLEQFGRP
                     LDLYNRPANLFVAGFIGSPRMNFLDGTVAEIGSDGVSIALKAGGTLKVPCSAGPSASA
                     SAVRLGVRPEHIALVENGTGDVPLSVSITEQLGGESYLHGALPSGETLSVRIPGQTRV
                     KRGETVGLTLAPPEHRHLFDATTGLVLPPLS"
     gene            complement(7397..8476)
                     /locus_tag="PK812_05525"
     CDS             complement(7397..8476)
                     /locus_tag="PK812_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017964241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRJ69052.1"
                     /translation="MIGTTDVTAFLARRRGRVGLHWSDWLAYAYLALGVLVVALPIMW
                     MFVSSVKSPRALVENDPRLLPYEQVTLHAADGQRPLGVFVWSKPDGTKADVAFVGPRG
                     ANARVFELATPDTIIEVPRTALARKEIVQPRWQNYTEPTIGSTQSRTFNFLRYFWNST
                     FVTVVATVLTLVVNAMAAFALSKYRFRGRDAMVIVIISTLLIPPTVILVPLFITVSTL
                     GMLNTLWGVIIPAIATPTGVFLLRQYMLTIPDELLEAARMDGASEWKIFWRIMLPLSL
                     PALSVLAILSVMWRWNDFMWPLIVLTQSEVFTLQLGLSTFKGELNDNMHHLLAMTVLT
                     LLPVTLVFVFLQRHITTGIANTGLK"
     gene            complement(8476..9711)
                     /locus_tag="PK812_05530"
     CDS             complement(8476..9711)
                     /locus_tag="PK812_05530"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRJ69053.1"
                     /translation="MTTATPSPKGAASGGALALWLWSRLGDALDGPFRAVQRHVGAHR
                     MAYVFLLPNLLVFGLFSFWPMILNFYIAFTGGQSVLLGERPFVGLENFRELLGCQSYL
                     DPKTCPVAGFSFWTGVWNTLFFALVQVPVMVVVALLTALVLNRDIRGRGFWRAVYFYP
                     VMLSPVVVAVIWDWVLKRRGILNEWLGDATDGWNALVPTIGFRLAAFALVGLLIHAAV
                     MRIARRLPEARRTLAARIAAALGSITVVTFVLTPQALTLPDYRPINWLIDVRSGWPMF
                     WVIFVYTWGHLGFYMLILLAGLQSIPKDLYEAAEMDGTSKGRQFWRITLPLLMPTMIV
                     VLVLSLIRAFQVFDEVYVLTGGGPGQATKMLIQHIYESAFIGDAKRYGIAAASSVLVA
                     GVLLVLTLLQLAATRKRAG"
     gene            complement(9776..11065)
                     /locus_tag="PK812_05535"
     CDS             complement(9776..11065)
                     /locus_tag="PK812_05535"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRJ69054.1"
                     /translation="MTTAWKGPGLLALGLTTSLFAATATPALAQTVNLRYLCYADANE
                     CDVARDLLDRFEKANPAIKVTVDKVGFAVIREQLETRLQGGEAPDMARVTNLGGLNKY
                     YLDLRPHINAANWEANFATTLPWMRVGPNDKGIYGWMTQLTVTGPFVNKTMFDQTGVK
                     MPGAGATWDDWAKALAEVQKKGKIYSGMALDRTGHRFAGIAISMGAKYFDAQGNPSAV
                     VDDGFKATAERVVQWHKDGLMPPDIWPGASGAKWKNAGDMFINSDTAMHIAGSWMIQK
                     FQSDIGNKFEWIVPPQPCGPAGCSAMPGGAAMVAFKSTKHPAEVAKVMEFMVSEPIIK
                     EYYERTVQIPAHKAVAEKGLTYGKEVGEQTQAALKQFTADFAKISPVAHQLQAYQRNV
                     AIFNATVNYVAQAITGPLTLQQAYPKIQEEIDNAVKK"
     gene            11207..12286
                     /locus_tag="PK812_05540"
     CDS             11207..12286
                     /locus_tag="PK812_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011927257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRJ69055.1"
                     /translation="MNRLADILNHRSRSAGAPARVSLSTIAARAGVSVSTVSRIVNGQ
                     LGRANPATVARVQALIEAYDYRPDKIARALRHGRSRVIAMLAPNLDNPAMGAIATSTE
                     AALRAAGYVMILCDTHDEPALQDEYLEAMRAQSVEAYVVVSAIASSALAAFIARGDPL
                     VLVGRRQPPGAPAAAFVGIDNRDAGRCAARFLIEAGVKAPALVHTTLTSSAVADRVLG
                     FRAGLEEAGVGRDCLRVAASSHLQHLHAGYEAARALFSAGGWPDGLFCVSDLMAYGTY
                     RLAREHGVRVPQDCILVGVDDSPLNEWIADWLTSVHIPYADYGSAILKQLEAVWAGQA
                     ASDLLLPHHLVNRLKARRKPLSDHD"
     gene            12279..>12895
                     /locus_tag="PK812_05545"
     CDS             12279..>12895
                     /locus_tag="PK812_05545"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HRJ69056.1"
                     /translation="MTDETAGLVRLLGGFRPSRFLCVGEEPLAAGLSGTRTLQLQLRR
                     NDGVLARAILTGPEQAWEGTPAVVYCHAHGGRYDIGAAELVASRPALRPEPYGLALAS
                     RGIVALAIDMPCFGVRSAETESAAAKRHLWRGTTLFGEMLEDLSGALDLLGDWPGIDR
                     ERIGAFGISMGATLAFWLAALEPRLKCIAHLCCFADLAELVRQGE"
BASE COUNT         2299 a         4049 c         4236 g         2311 t
ORIGIN      
        1 caggcacaag atgtcgaggc gcggcttgac gcgacgcggc gcgaacgcag cgacacggat
       61 ggactgcccg cagaattgct ggcccgccgc cgccttctcg ccggcgagat cgaaagcgcg
      121 gaaggagcgc gcaaggtagc ggccgacgca cgcgctgagg gtgaaacgat tctgctgacc
      181 ttcgaccgcg tggcgcgaga agcgctgacg gcgctttcga acgtccgcga acagcgcgcg
      241 cggtcggaaa cgcagctgga ggccgcgaag ggccggcttg tcgacctgac ccgtgagatc
      301 gaggaaacgc ttggcgtatc gccgtcgacg ctgggtgaaa tcagcggtat tgcccgaccg
      361 gaagaccttc cggccgcgca ggctgcgcag gccaggctgg atgatctcag gcgcgagcgc
      421 gagcggatcg gcgcggtcaa tctgcgtgcg gatcaggaac tggccgaggt cgtggcgacc
      481 cgcgcggatc tggctcgaga acacgacgaa ctgggcgagg cgatccgcaa gctccggcgc
      541 ggcatcgaca gcctcaacgc cgaggggcgc aaccgactgg ttgccgcctt cgaagtggtg
      601 aacggacatt tcaagaccct gttcacgcgt ctgttcggcg gcggctcggc ggagttgcag
      661 ctcatcgaat cggacgatcc gctcgaggcg ggtctcgaga tcatggctca tcctccggga
      721 aagaagccgc aagtgctcac gctgctgtct ggcggcgaac aggcgctgac cgccacggcc
      781 ctgatcttcg ccgtgttcct caccaatccc tcgccagtct gcgtgctgga cgaggttgac
      841 gcgccgctgg acgattcgaa tgtggaacgg ctctgcgatc tgcttgccga catggctcgg
      901 gaaaccgaga cccggttcgt catcatcacg cacaatccga tcaccatggc gcgcatggac
      961 cggctgttcg gagtcaccat ggccgaacgc ggcgtgagcc agcttgtctc ggtcgatctg
     1021 gaggcggcgg agcgcctggc cgaagccagc tgacggcggg cgccattccg gcccggatcg
     1081 cggctccgtc atgccgcact gcggcaacat tggtctaatg cgatgatttg atgtgttttt
     1141 tcaatgcgtt agatatttgc ctggacgctt gacacaaggt gagggcgaaa atatggtgcg
     1201 cgcgctttca aggggccgtt tctcagccct tccgcctcct gccctgacgg cttttgaagg
     1261 tccggcgcgc ccgacggcgc aacggccgac gcctgaaagg agaggggatg gataaggacg
     1321 gaacaagccc caaagccggc gaggatgcgc tgcaggcgcg cctcgacgag ttgacgaaag
     1381 cgcttgacgc caagcgtcaa ggcagcgaag aggccgcgcg atccaaatca tcaggatcgg
     1441 acacgggtat cggcaaggcg atgggggcgg gcttcagagt cgcttccgaa ctcgctgcag
     1501 ccatcctggt cggtggtttt ctgggctggc agctggacct ctggttcggc agcacaccct
     1561 ggtttctcat gatcttcctg atcctgggaa tcgtcgcagg gttctggaac gtataccgtc
     1621 tcgccgccaa gcccaccggg ccgatggcgg gcacggacaa gtgaattcgg gcagggtccg
     1681 catccggagc ggaacctgaa cttggctcca aggggttcaa tcgtggcggc tggcggcgcg
     1741 gtaatcgatc cgattcatca attcgagatc aagaagatca cgaacatcac tgtcggtggg
     1801 ttggacctgt cgttcaccaa cgcttcgctg ttcatggtca tcgcgctcgg cgtgatttcg
     1861 ctgatcatga tctggggcac ttcggcgcgc gcgatcgtgc ctggtcgcct gcaatccatt
     1921 gccgagatgc tctacgagtt catcgaggac accgtccagg gcacgatggg caagtcgggt
     1981 cgcaagttcc tcccgctggt gttctcgctg ttcatgttcg tcctggtctg caacatggtc
     2041 ggcctgatcc cgggcacctt taccgttacc agccatatca tcgtcacctt cgcccttgcg
     2101 gcggtggtga tcctgacggt gctgatctac ggcatcatga agcacggcac gcatttcttc
     2161 ggcctcttcg tcccctcggg cgtgccgaag ccgctgctgc tgctgctgac gccgatcgag
     2221 atcatttctt ttgtgtcgcg cccgatcagc ctttcgatgc gtctgttcgc caacatgctg
     2281 gcgggacaca ttgcgctgaa agtgttcgcg ggcttcattc tgatgatgac cgccgccggc
     2341 ggcgtggcag tcgcgctgtc gccgctgccg ctgctgatga tcgtggcgct gaccgcgctg
     2401 gaattcctcg ttgcggcctt gcaggccttc gtcttctcgg ttctgacctg catctacctc
     2461 aacgacgcgc ttcacccggg ccattgatcg gcccaaaccc aacatccaaa catcactgaa
     2521 actcaaggag ttctaccatg gatgcgaatg ctgcgaagct catcggcgcc ggtctggccg
     2581 ctatcgggat gggcgccgcc gccatcggcg tgggcacgat tttcggcaac ttcctctcgg
     2641 gcgcgctgcg caatccgtcg gcggctgacg gccagttcac gcgcgcgttc atcggcgccg
     2701 cgctcgccga aggtctgggc atcttcgcct tcgtcgtcgc gctgatcctg ctcttcgtga
     2761 agtgagcggc cccgccgggg tcgtttattt ccttgattga aaccgggtcg gcggcagggc
     2821 tcctgcaccg gcccgatccg agcaaaacag gtcggccatg tccttgttca tcaccgccgc
     2881 acatgcggcc agcggtgcag gcggcggagg agcgttccct cctttcgacg cttcgacctt
     2941 cgccgggcag ctgttctggc tggctgttgt attcggtgct ctctattggc tgatggccaa
     3001 tgtggcgctg ccgcgcgttg cctcgatcat cgaggatcgc cgcgcgcgca tcgccgccga
     3061 tctcgacacg gccgccacag cccagaaggc tgcggcggat gcccagaagg cattcgaggc
     3121 caacctggcg caggcgaagg ccaaggctca gggcatcgcc cagtccgcgc gcgacgcggc
     3181 gtcccgcgag gccgacgcac gccgccatca ggttgaaact gacctgacgg ccaagatggt
     3241 ggcagccgaa aaggcgatcg ccgagaccaa ggccaaggcc atggcgaatg tcgaaggcat
     3301 cgccacggac gccgccgcgg ccatcgttga acgccttggg ggaagcaagc cgggcgacaa
     3361 ggcaatcact gacgccgttg ccgcgctgtc gcagaggtaa ggagacacgg atgtctgata
     3421 cgctctgggt cgccgtcgga tttgtgatct tcctcggcat tgtcgcctac tatggtggcc
     3481 acaaagccct gctttcgggg atcgatggcc gcgcacagaa gatcgccgcc gaactcgccg
     3541 aggccaagcg cctgcgcgag gaggccgagg cgctgatggc cagcttcaag gccaagcagg
     3601 cgcaggccga gaaggacgcc gaggcgatga tcgcagccgc caaggccgag gccgagcgcc
     3661 tcaaggttga ggcggggcag aaactcgacg atttcgtccg tcgccgcacc gctcaggcgg
     3721 agctgaagat tgcgcaggcc gaagcgcagg cttccgccga cgttagggcc gccgcagccg
     3781 atctggccgc agcggtcgcc ggcaaggtgc tggcagggca aggcgcaagc gactcggcct
     3841 tcagcgcggc ccttggcgag atcaaggcga aaatgaactg acggttcccg tccatacccg
     3901 ggatcagacc gttctccgag ccggccgttc aggccggctc gatgcttatg tggcgccctt
     3961 tcagcccgct tcccgcgaca gaccagttcg acgccacgcc ggcattccag gccgatccgg
     4021 ctgaaggtgt caggcggacc cgcgcctcgt cggccgtcca ggtcaggcgg atgctcagga
     4081 ccagtgcgtt gccggcctgg aaatcccaac tcgtgccatc atcctcgaaa tggatgccgc
     4141 cgccctcgcc cgacccgggc gcgagagaaa gatagagctg gcgcgccggc gcatcatgcg
     4201 ggcgcacatg aggcagcgcg ctcgccagga ccagcgccgc gccaacgcgc acgaagaccg
     4261 caggacagcc gagatcggca gcgaccgtca cgttctgacc ggcggcgtgt acttgtccgc
     4321 tgtggaagtc gacccagcct gacgggccga gcggaaggta ctgtgtcttc acccgagcgc
     4381 ctggctcgac gaccggcgag aacagcacat ccggccccag catcatcgcg tcctggtccc
     4441 ggtaggcgtc tggatcattg gcgaagtcat ggaacagggg ccgaatcatc ggctcgcctt
     4501 tggcatgata gcgctgcgcc agcgtgtaga tcagcggcag gaagcgggtc cgaaggtcga
     4561 gggcggcgcg aatctgtggg agaacctcgg gataaagcca aggcaccgtc gcggcggtcc
     4621 agtccggcgc tacgtgtggc ttccaggaat tcatgaccgc ccgcgggtgc agtgccatca
     4681 tctcgatgaa gcggcagagc agttcgccat cgggacgggg accggtgaaa ccgccgatgt
     4741 cgtggccgac gttcgactga ccggacaggg ccatcgacag gccgttgcgc aggttccatt
     4801 tcaacgtgtg ccaggacgtg gcgttgtcgc cggtccatgt ctcggcatag cggtgcaggc
     4861 cgacaggacc ggcgcgcgtg atcgtatagg ggcgcgatgc cggcgcacgg gcccggcttg
     4921 cctcgaaggt agcccgtgtc atcaggagcg catggagcgg acgcgccgcc gtggcgggaa
     4981 acggccggcc atgcccggtc gcgatcgcat cctcctggcc gatctcgtat tcattgttgt
     5041 cgttccagcc cgcatcgaat ccgatgtcga gcacctgctc gaccaggcgc tgctgccacc
     5101 aatgggcggt ctgctcattg gtgaagtcga gatacgaccc catgccgtcc cagaactgtt
     5161 cgagcaccgg cgcgccatcg cgcgccttca caaagccgcc ggctgccttg accgacgcat
     5221 agtcgggatg atcatcgatc agtacgggct ttaggttggc gacggtataa aatccgagcg
     5281 ccttcagctt ggcgaacaag gccgatgggt cagggaactt ctgccggttc caggtgaaga
     5341 catagcgccg cttgccgcgg ctggaatatc cggagccaaa gtggatcgcg ctgattgcga
     5401 tctcctcctt gcggcaacgt tccgcaaagg cggcgataac ctgctgcgcc tcggcatcgt
     5461 ccgcgtggtg catcgtggtg aaggcaaatc catagctcca gcgcggcgga aatgcggggc
     5521 gcccggtcag catcgtgaag cgccgtgtcg tcgccgcgag atcggggccg gcgacgagcc
     5581 agagatcgag tccgcgttcc tcggcttcga catagcggta gatgccgtgg tagttcgagc
     5641 gttcgcagcc aagatcgaac gtcatcggcg cgagcgtatc gtagaacagg ccaccgcata
     5701 cgtcaccgga ctgcaccgcc atgtacggcg tatgcttgta gagcggatcg ccgatctccg
     5761 catcatagcc gagggcgtca agttgcagca ggcggaagcg tcgccccgtc cggtccagcg
     5821 gaccggtctt gtcgccgagg ccgaaatgcc ggtcgttgtc caggcgcatc tggaagtgcc
     5881 ggattcgccg gtcgccgtcg cacagcgcca gtccgccggt cgcgcggtcc cgcagccagg
     5941 gtttccagtc gtcgccgacc cgctgctcaa cgtccattcg gacagggttg ccacgcagcg
     6001 tcacccgaaa ccggcctgcg gtcaggatat ggtcgccatt gcttgtcgtg aggtcagcgg
     6061 cgacgggcga aaagccagca agcgactcgc ggctgcgacc ctcggccggt gtatcgccct
     6121 cgggcgcgat catccaggtg cggtcgaggg ccaggccttc gggtggcgcc acgacgatcc
     6181 gcaagagcgt ctccgacatg gcgcggacgc gcagcgtcca gccctcgtcc agggaaagat
     6241 caaagccgtt cgagtgcgct gtcggcgcgc caagtgtttg aaagatgcgc atatctggct
     6301 caggacaggg gcggaagtac gaggcctgtg gtggcatcga acagatggcg gtgttcaggt
     6361 ggggcgagcg tcagcccaac agtttcgccg cgcttgaccc gggtctgccc gggaattcgg
     6421 accgagagcg tctcccccga cggcagggcg ccatgcaggt agctctcgcc gccgagttgc
     6481 tcggtgatgg agaccgacag cggcacatcg ccagttccgt tctcgacaag tgcgatatgc
     6541 tcgggccgca cccccaaacg cacggcagaa gcgctggcag acggcccagc cgagcagggc
     6601 accttgagcg ttccgcctgc tttcagcgcg atgctgacac cgtccgagcc gatttcggcg
     6661 accgtcccat cgagaaagtt catgcgggga gagccgatga agccagccac gaacaggttt
     6721 gccggccggt tgtaaaggtc gagcgggcgg ccgaactgct ccagccgccc gtctttaagg
     6781 acggcaatgc ggtcggccat ggtcatcgct tccacctgat cgtgggtaac gtagatcatc
     6841 gtgttgccca gccgctgatg caggttgccg atctcgatcc gcatctgcac ccgcagttcc
     6901 gcgtcgagat tcgacagcgg ttcgtcgaac aggaagattt tcggttcgcg caccacggcg
     6961 cgaccgatcg cgacgcgctg gcgctgaccg cctgacagtt gcccggggcg gcgatccagc
     7021 agatgttcca tccgcagcat gcgagccgct tccgccacgc gccgctgaat ctccggcttc
     7081 ggggtcttga ggttctccag accaaatgaa aggttctggc gcacgctcat gtgcggatag
     7141 agcgcatagc tctggaatac catggcgagt ccacgttcgg ccgccggaac gtcgttgacc
     7201 aacgcgccat caatctcgat cgcgccgccg gtcacgcttt ccagtcctgc gatgagacgc
     7261 agcagcgtgg actttccgca gccggacggg ccaacgaaga cgatgaactc tccgtgacca
     7321 atcgacaggt caacgccatg gatgactttg gtggcgccaa aagacttcgt cacctgtctg
     7381 agatcgagtg tcgccatcac ttcagtcctg tattggcgat gcccgtcgtg atgtgccgtt
     7441 gcaggaacac gaacaccagc gtcaccggca agagcgtcag caccgtcatc gcaagcaggt
     7501 ggtgcatgtt gtcgttgagc tcgcccttga aggttgaaag gccgagctgg agcgtgaaga
     7561 cttccgactg ggtcaggacg atcagcggcc acatgaaatc gttccagcgc cacatgaccg
     7621 acaggatcgc caggacagac agcgcaggca ggctcaacgg cagcatgatg cgccagaaga
     7681 ttttccactc ggaagcgccg tccatgcggg ccgcctcgag caactcatcg ggaatggtca
     7741 gcatgtactg gcgcagcagg aacacgccgg tcggcgtcgc gatggccgga atgatgacgc
     7801 cccagagcgt gttcagcatg cccagcgtgc tgaccgtgat gaacagcggc accagaatga
     7861 cggtcggcgg gatcagcagc gtcgagatga tgacgatcac catcgcgtcg cgtccccgga
     7921 accggtactt tgacaaggcg aaggcggcca tggcgttgac cacgagggtc agcacggttg
     7981 ccacgacggt gacgaaggtc gagttccaga agtagcgcag gaagttgaag gtgcgcgact
     8041 gcgtcgaacc gatggtcggc tccgtgtagt tctgccagcg cggctggacg atctccttgc
     8101 gggccagcgc cgtgcgtggg acctcgatga tcgtgtcggg cgttgccagt tcgaagacgc
     8161 gggcattggc gccgcgtggg ccgacgaacg cgacgtcggc cttggtcccg tccggctttg
     8221 accagacgaa aacgccaagc ggccgctggc cgtcagccgc gtgcagggtc acctgctcgt
     8281 agggcagcag gcgcgggtca ttctcgacca gagcacgcgg cgacttcacc gacgagacga
     8341 acatccacat gatcggcagc gccacgacca gcacgccaag cgcgaggtag gcataggcga
     8401 gccagtcgct ccagtgcagg ccgacgcgcc cgcgtcgtcg cgccaggaat gcggtgacgt
     8461 ccgtcgtccc gatcatcagc cagccctctt tcgtgtcgcc gcaagctgaa ggagggtcag
     8521 gaccagcagc acgccggcaa ccagcaccga cgaggccgcc gcgatcccat accgcttggc
     8581 atcgccgatg aaggcgctct cgtagatgtg ctggatcagc atcttggtcg cctgacccgg
     8641 accgccgccg gtcaggacat agacctcgtc gaaaacctgg aaggcgcgga tcagcgacag
     8701 caccagcacg acgatcattg tcggcatcag cagtggtagg gtgatgcgcc agaactggcg
     8761 gcctttcgac gttccgtcca tttccgccgc ctcgtagagg tccttgggga tcgactgcag
     8821 gccggcgagc aggatcagca tgtagaagcc caggtgcccc caggtgtaga cgaaaatgac
     8881 ccagaacatc ggccagccgg agcgcacgtc gatcagccag ttgatcggac ggtagtcggg
     8941 aagggtcagg gcctgcggcg tgaggacgaa cgtcacgact gtgatcgatc cgagtgcggc
     9001 tgcgatcctg gctgcgagcg tgcgccgtgc ctcaggcagt cggcgggcga tgcgcatgac
     9061 ggcagcgtga atcagcagcc cgacaagcgc gaaggccgca agtcgaaacc cgatggtcgg
     9121 aaccagcgca ttccagccgt ccgtcgcatc tcccagccac tcgttgagaa tgccgcgacg
     9181 cttgagcacc cagtcccaga tcacggcgac gacaaccggc gacagcatga cggggtagaa
     9241 atagacggcg cgccagaagc cgcgaccgcg aatgtcgcgg ttgagcacca gggcagtgag
     9301 cagtgccacg acgaccatca cgggcacctg aactagggcg aagaacagcg tgttccagac
     9361 gccggtccag aacgaaaatc cggcgaccgg acaggtcttg gggtcgaggt agctttgaca
     9421 gccaaggagt tcccggaagt tctccagccc gacgaaggga cgctcgccga gcagcactga
     9481 ctgtccgcca gtgaaggcga tgtagaaatt gagaatcatc ggccagaagg aaaacaggcc
     9541 gaaaaccaga aggttgggca agagaaagac ataggccatg cggtgcgcgc cgacatggcg
     9601 ttgcaccgcg cggaatggcc cgtcgagcgc gtcgccgagc cggctccaga gccatagcgc
     9661 cagagcgccg ccgctggcgg cgcccttggg gcttggagtc gccgttgtca tggccctgtc
     9721 ctgtccggtt aggggacggc gctccgcccg gcgccgtggc cgggcggcag acgctttact
     9781 tcttcacggc gttatcgatc tcttcctgga tcttgggata ggcctgctgc agcgtcagcg
     9841 gtcccgtaat cgcctgcgcc acgtagttca cggtggcgtt gaagatggcg acgttgcgct
     9901 gataggcctg aagctgatgc gcaaccggcg agatcttggc gaagtcggca gtgaactgct
     9961 tcagcgccgc ctgcgtctgt tcgccgacct ccttgccgta ggtcaggcct ttctcggcaa
    10021 ccgccttgtg cgcggggatc tgcacagtgc gctcatagta ttccttgatg atcggctcgg
    10081 agaccatgaa ctccatcacc ttggcgacct ccgccgggtg cttggtcgac ttgaatgcca
    10141 ccatcgcagc gccacccggc atggccgaac agccggccgg gccgcagggc tgcggcggca
    10201 cgatccactc gaacttgttg ccgatgtcgc tttggaactt ctggatcatc caggagccgg
    10261 cgatatgcat cgcggtgtcg ctgttgatga acatgtcgcc ggcgttcttc cacttggcgc
    10321 ccgatgcccc cggccagata tccgggggca taagcccgtc cttgtgccat tgcacgacgc
    10381 gctcggccgt cgccttgaag ccatcgtcga ccacggcgct cggattgccc tgtgcgtcga
    10441 aatacttggc gcccatcgag atggcgatgc cagcgaagcg gtgccctgtg cgatccagcg
    10501 ccatgccgga ataaatctta cccttcttct gcacctcggc cagcgccttg gcccagtcat
    10561 cccaggtcgc gcctgcgccg ggcatcttca cgccggtctg gtcgaacatg gtcttgttga
    10621 cgaaggggcc ggtcacggtg agctgggtca tccagccgta gatgcccttg tcgttggggc
    10681 cgacacgcat ccacggcagc gtggtggcga agttcgcctc ccagttcgcg gcgttgatgt
    10741 gcggacgcag gtccagatag tacttgttta gtccgccgag attggtgacg cgcgccatgt
    10801 ctggcgcctc gccaccctgc aggcgtgtct ccagctgttc gcggatgacc gcgaagccga
    10861 ccttgtccac cgttaccttg atggccgggt tggccttctc gaaccggtcg agcagatcgc
    10921 gcgccacgtc gcattcgttg gcgtcggcgt agcacaggta ccggagattg accgtctgcg
    10981 ccagggcggg ggtcgccgtc gctgcaaaga gtgaagtcgt cagtccgagc gcgagcagcc
    11041 ccgggccttt ccaggcagtc gtcatgatga agtcctccca atatgcccgc cggccgggcg
    11101 aattttccat cgctggcccg gtacggatcg caagccgaaa agcgattggc gggagtttgg
    11161 caaacgtttg ccaagtaagt caagctgcaa taccgttgac gtcgtcatga atcgtctggc
    11221 tgatatcctg aaccatcgat cacgatccgc cggcgcgccc gcgcgcgtct cgctctcgac
    11281 cattgcggcg cgggccggcg tctccgtctc gaccgtgtcg cgcattgtca acgggcagtt
    11341 ggggcgtgcc aatccggcga cggttgcgcg ggtgcaggcg ctcatcgaag cttatgacta
    11401 tcgtccggac aagatcgcac gggctttgcg ccatgggcgc agccgggtca tcgcgatgct
    11461 ggcgcccaac ctcgacaacc cggcgatggg ggccatcgcg acctcgaccg aagccgcgct
    11521 tcgtgcggcg ggctatgtca tgatcctctg cgatacccat gacgaacccg cgcttcagga
    11581 cgaatatctc gaagccatgc gcgcccagtc agtcgaggcc tatgtggtcg tctctgcgat
    11641 tgcgagctcc gcgctggcag ccttcatcgc ccggggtgat cctcttgtgc tggttggccg
    11701 tcgccagcct ccgggagcac cggcggcggc tttcgtcggc atcgacaatc gtgatgccgg
    11761 acgctgcgca gcccgcttcc tgatcgaagc cggggtcaag gcgcccgcgc tggtgcacac
    11821 cacgctgacg tcctccgcag ttgctgaccg cgttcttggt tttcgtgccg ggcttgagga
    11881 ggctggtgtc ggccgggatt gcctgcgtgt cgcggcttcc agccatcttc agcatctgca
    11941 tgccgggtat gaggcagccc gggcgctgtt ttccgcaggt ggctggccag atggcttgtt
    12001 ctgcgtcagt gacctcatgg cctacggcac ataccgtctg gcgcgtgagc atggtgttcg
    12061 ggtgccgcag gattgcattc tggtcggcgt cgacgacagc cctctgaacg agtggatcgc
    12121 cgactggttg acgtcggtgc atatcccgta cgccgactat ggttcggcaa tcctgaagca
    12181 gctcgaagcg gtttgggcgg gccaggcggc aagcgatctc ctcttgccgc atcatctggt
    12241 caatcggctg aaggcgaggc gcaagccttt gtcggatcat gactgacgaa acggccggcc
    12301 ttgtccgcct gctgggcggg tttcgtccat cgcgtttctt gtgcgttggg gaagagccgc
    12361 tggcggcagg actttccggc acccgcacgc tgcaactcca gctcaggcgg aacgacggcg
    12421 ttctggcccg ggccatcctg accggaccgg aacaggcatg ggagggaacg ccggctgttg
    12481 tctactgtca tgcccatggt gggcgctacg acatcggcgc agcggaactt gtcgcctcac
    12541 gtcctgccct gcggcctgag ccatacggtt tggctttggc ctcgcgggga atcgtggcgc
    12601 tggccataga catgccatgc ttcggtgtcc gctcggccga aacggaaagc gcggctgcga
    12661 aacggcatct gtggcgcggc acgacgctat tcggcgagat gctggaggat ctctccggcg
    12721 cgctcgatct gcttggcgat tggcctggaa tagaccggga gcggattggc gcattcggca
    12781 tttccatggg ggcgacgctg gcgttctggc tggccgcgct ggagccccgc ctaaagtgta
    12841 tcgcgcatct gtgctgcttt gccgaccttg ccgaactggt cagacagggc gaaca
//
LOCUS       DAOMXN010000047        12533 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_2737_length_12533_cov_10.656275, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000047 DAOMXN010000000
VERSION     DAOMXN010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 12533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12533
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2737_length_12533_cov_10.656275"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..1137)
                     /locus_tag="PK812_05550"
     CDS             complement(130..1137)
                     /locus_tag="PK812_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HRJ69057.1"
                     /translation="MPASGDILQQLRRPGGALLAPMSGITDAGMRRAALRFGATLVVS
                     EMVASDQLALGEEEARLRAEGDGIVPHVVQIAGCEPRWMAEGARAAEAAGAAIIDINM
                     GCPAKRVTNGWSGSALMRDLDNACRLIDATVSAVSVPVTLKTRLGWDHANLNAPELAR
                     RAEALGVRMITIHGRTRQQFYKGRADWKAVLAIREATTVPLVVNGDIADARSARSALS
                     ASGAEHVMIGRGALGRPWLVGEIAAELAGIAKPLLDGHVLADAALAHYEFLLRAFGVE
                     KGLRHARKHVVAYHDEAIRRGGQGGDRAALATCEDPRAVMRLLRAAFESIPAVHLSEV
                     A"
     gene            1299..2495
                     /locus_tag="PK812_05555"
     CDS             1299..2495
                     /locus_tag="PK812_05555"
                     /EC_number="2.7.7.60"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012496039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase/2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase"
                     /protein_id="HRJ69058.1"
                     /translation="MTEPVIAALIVAAGRGSRIGGYTAKQYLKVGGRSILARAIDGFV
                     AHPMIERIQIVIHPDDQDLFDTVLAELTPRPAKPIEACGGGASRQESALLGLRALKAT
                     LAGDSTCVLVHDAARPFLSEALITRAIAAAWRHGAAIPVLPVVDTVAMLDSAGDLAGN
                     ADRGALRLVQTPQAFRFGPLLVAHEAAAQQGRSDFTDDASLVRWRGATVATFAGDPAA
                     FKITTPEDLARAEALAGARLADIRCATGYDVHAFGPGNSIMLGGVKIAHARGVIGHSD
                     ADVLLHALTDALLGCIGDGDIGVHFPPSDMRWKGAASRVFLEEAVRRVHARGGSIRHL
                     DGTIVCEAPRIGPHREAIRQTVAAIAGIDCNRVSLKATTSEKLGFTGRGEGIAALATA
                     TIALPE"
     gene            2506..3018
                     /locus_tag="PK812_05560"
     CDS             2506..3018
                     /locus_tag="PK812_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HRJ69059.1"
                     /translation="MPDTKSPSHLLAVEVLKLARARGERLVTAESCTGGMVAAALTDI
                     AGSSDAFDRGYVTYSNDAKQAMLSVPEKLLKGYGAVSAEVAAAMALGALRHSGAHLAV
                     AITGIAGPGGGTAKKPVGLVHFAVVHRDGRVDRVEKRFGNLGRAEIRARSVETSLDML
                     RAMLAAPAVA"
     gene            complement(3045..5318)
                     /locus_tag="PK812_05565"
     CDS             complement(3045..5318)
                     /locus_tag="PK812_05565"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HRJ69060.1"
                     /translation="MTSIDPVYASAHGRRGTASHGVVLAAFISAIFTSAFLLFAVQPM
                     FTKMVLPLLGGSPGVWSVAMVFFQALLLAGYAYAHVSTKYLTMRQATLAHLVLFGVTA
                     FALPIGVSQSLGEPPTQWQEIWLIGVFCASVGLPFFAVSANGPLLQAWFARSGHPTAQ
                     DPYYLYGASNLGSFAALLLYPVLIEPLLTLKGQTYAWSFGFCVLGALIGLAGVLVSRN
                     LDAKAQRGTADIVDYSPAPTRARMAQWIGLAFVPSGLLVAVTAHVSTDVAASPFLWVI
                     PLALYLATFVVVFRDPMPFDIGSLDRILPIVATSLALFLFLGAEMLWASMLVHFGFMT
                     LATLICHARLYALRPNPQHLTTFYLCMSFGGVLGGLFCGLIAPNVFNSIAEYPILIIA
                     TFLCLPAVYAASRPQWVREAAPWLIGLMLVQLVVAKQPQLFTGIAKNLLVSLLLATIG
                     IALVLYRRPLVVGAICAGVFPLIGSFSLNTRDELTLRSFFGVHKVQVTNDGQYRNLAH
                     GTTLHGSMRIKSPEGAPLPFRPEPLAYYARSGPMAESIRSVQTRANGAGRSFAVVGLG
                     AGSIVCHGRNNDQWTYFEIDPAVVRIATDPGYFRFLSVCAPEARMVLGDARLTLAKEN
                     KPYDALLIDAFASDAIPVHLLTREAVQMYLSKIKTDGLLVLHISNRYLDLESVVAAIA
                     SDLGVEGRAKLDAVQKDEHLASGKAGSHVIALSHSPEALRALEDYGWRNLREGGVTAW
                     TDDYSNIVGAMWRKAWQ"
     gene            complement(5374..5841)
                     /locus_tag="PK812_05570"
     CDS             complement(5374..5841)
                     /locus_tag="PK812_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HRJ69061.1"
                     /translation="MPHFSTTRRVPHTATAMLELVADVERYPEFLPYCQELEVLRRVD
                     APDGRHILTARMTIGYKMIHESFTSRVTVDRARRRILVEYIDGPFRHLENRWLFEDSG
                     EGGSKVDFSIDYSFRSRAFEILAGAVFDKLFRKMAESFEERADALERSRRAQA"
     gene            complement(5878..7956)
                     /locus_tag="PK812_05575"
     CDS             complement(5878..7956)
                     /locus_tag="PK812_05575"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HRJ69062.1"
                     /translation="MIDRLLSFSPLARRAVRADAAVLLLFIAASGLSAALLFAIQPMF
                     TRMVLPRFGGSASVWSVAMVFFQAMLLAGYAYAHVLARFVPLRFALVLHAVVLALGLA
                     FLPLQPRFEAAFGNEESLRLLVLLTISIGVPFVALSANAPLLQICFANSGAPRAGNPY
                     PLYAVSNAGSLAALLAYPSFLEPLLTLRQQALGWSVGYGALLLLLLLAALAMLKAPAL
                     PIRRARPHREASWSEILRWTGLALTPSAALVAVTLHLSTDIVAAPFLWVAPLALYLAT
                     FIIAFGVSGTAWLDKLGKVLGPSLLVLALLLTFEVRTWPLLDVLLHLTVFFLLALYSH
                     GRLAACRPDPGGLTGFYLSMSSGGVLGGALCALAAPSLFTFVAEYPIALALAAFSLPR
                     FGPALAAILVAVLLFGRMVPLEVRERETLRSFYGVHTIETTADGQFRSLRHGVEVHGA
                     QQIADRAGRPVRGKPVPLTYYSEDSPISEAIDAARTKAGGGALRVGVVGLGAGSIACL
                     VEPSDTLHFYEIDPVVIGIARDRTKFRFLSDCAPNAAIIAGDARLTLEREAATYDILI
                     LDAFSSDSIPAHLLTREALSSFVVRLAPQGTLLLHISSNHLRLQEVVAATALSLELAV
                     RINDEEQGDGAPAMTIQPTVAVLARKSEHFGVLNDNDDWQAPGVRLPRPWTDDYANPL
                     GAFLAKLRER"
     gene            complement(8127..8762)
                     /locus_tag="PK812_05580"
     CDS             complement(8127..8762)
                     /locus_tag="PK812_05580"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HRJ69063.1"
                     /translation="MSAESTLRDSMVRLAKSLFERGFTVGSSGNISARLDDGMLITPT
                     NSCLGFLDPARISKLDAGGRHLAGDKPSKEVFLHRAFYETRPAAGAVVHLHSTYATAI
                     SCLAESDADDCIPPLTPYVVMRVGRVRLLPYVRPGDPAMGDMIRALEGRHAAVLLANH
                     GPVVSARDLESAVFAAEELEETARLVLLLRGHPTRRLTQAQVEELSRVFGG"
     gene            complement(8759..10039)
                     /locus_tag="PK812_05585"
     CDS             complement(8759..10039)
                     /locus_tag="PK812_05585"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019965617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HRJ69064.1"
                     /translation="MLLGVIADDYTGASDIANTIAKGGGGTPGLATVQFLGIPKGRAP
                     ESCEAGVVALKSRSIPAGDAVAQSLAALRWLQAQGCRQIVFKYCSTFDSTPDGNIGPV
                     AEALARALGVHGVVACPAFPGAGRTVYQGHLFVGDRLLSQSGLENHPLNPMTDADIRR
                     WLRRQTETPVGIASWNATVAKGAAALQDALAEAAGRGETLVIVDAISDDDLLTIGEAV
                     ADAPLLTGGSGVALGLSRNFIRKGLAKGAASAFRRVDGPEAILAGSCSRATLGQIAHH
                     KAQGHPVLPIDVAGVMSGAVVMADLVGFLVRNAGRAPLVFSSSPPDEVAALQARFGRE
                     AVAHRLDSLFAETARAAVDGGIRRLVVAGGETSGAVVSALGLEALAIGPEIDPGVPAL
                     SNASGVPLAVALKSGNFGAVDFFAKALATLGGEA"
     gene            complement(10158..10247)
                     /locus_tag="PK812_05590"
     tRNA            complement(10158..10247)
                     /locus_tag="PK812_05590"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10211..10213),aa:Ser,seq:tga)
     gene            complement(10309..11274)
                     /locus_tag="PK812_05595"
     CDS             complement(10309..11274)
                     /locus_tag="PK812_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HRJ69065.1"
                     /translation="MAEVLTLAFPFFGLILLGFFCGKLMRFPEEGLRWMNFFIVYVAL
                     PPLFFKLIAATPFEELKNWPFVIGTTLSTYLAFVISLAIGLWASRGDLRPATIQAVLG
                     SYSNIGYMGPGLTLAALGSKAAVPTALIFVFDNALLFTLVPFLMAMGSSGRLNVWETT
                     KLVIWRVVSHPFNIATFVAVLAAYFQIKVPTPVDTMITYLKNAAAPVALFTLGVTVAL
                     RPLTSVPFEVPAHLLVKLVVHPLIVLVLLSLIGGFEREWVFAAVLMAALPPALNVFVL
                     ARQYDVYVERASTGILVGTLVSTLTVTGLLYLIATDTLPYSVFGR"
     gene            11516..11845
                     /gene="hspQ"
                     /locus_tag="PK812_05600"
     CDS             11516..11845
                     /gene="hspQ"
                     /locus_tag="PK812_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HRJ69066.1"
                     /translation="MPARDAKYRIGQVVRHRIYPFRGVVFDIDPEFANTEEWWQSIPP
                     EVRPRKDQPFYHLYAENDETEYVAYVSEQNLIPDTSKEPLRHPQVREVFVKNRDGSFR
                     MKSGVFH"
     gene            complement(11952..>12533)
                     /locus_tag="PK812_05605"
     CDS             complement(11952..>12533)
                     /locus_tag="PK812_05605"
                     /inference="COORDINATES: protein motif:HMM:NF018482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HRJ69067.1"
                     /translation="APAAPQGQQPAAPAGPTLVNLKADAAQEWTKVCGEDPAAKKTIC
                     YTTRDFLSDNNQPIMAVAVYDVQNDPKKFVRFLLPLSFLLQPGIRFGVDSAQPVSGRF
                     QICFPNGCFAEAEVNEAFLANLKKGTNVKVSVQNDRGQEIAFVVPLAGFGKAFDGAPI
                     DPKVLEQQRKMLEEALQKEAEELRKKQGEAPKQ"
BASE COUNT         2475 a         4008 c         4094 g         1956 t
ORIGIN      
        1 gcgcgcggct cagatcgaag aagtgctcgc tggcctggtt ggcttcgatg atgtgtccgt
       61 ccggtccgac gcagaacacc ggattcggta cggaatccag caaagaacgg tctgaggcgg
      121 ttgatccggt catgcgacct ccgacaggtg aacagccggg atgctctcga aggccgcccg
      181 caatagccgc atcaccgcgc gaggatcctc gcaggttgcc agtgccgcgc gatccccgcc
      241 ctgcccgccg cggcggatgg cctcgtcatg gtaggcgacc acgtgtttcc gggcatggcg
      301 caggcctttc tcgaccccga acgcgcggag caggaactca taatgcgcga gggcggcgtc
      361 cgcgagcacg tgcccatcca gcaacggttt ggcgattccc gccagctccg ccgcgatttc
      421 gcccaccagc cagggccgtc cgagggcccc gcgcccgatc atcacatgtt cggcaccgga
      481 agcggacagc gccgaacgcg ccgaacgcgc atcggcaatg tccccgttta cgaccagcgg
      541 cacggtcgtc gcctcccgga tggcgaggac ggctttccag tccgcccgcc ccttgtaaaa
      601 ttgctggcgg gtgcggccgt ggatcgtgat cattcgaacg cccagcgcct cggcacggcg
      661 ggcgagctcg ggagcattga ggtttgcgtg atcccagcca agccgggttt ttagcgtaac
      721 cggtacggaa acagccgaga ccgtggcatc gatcagccgg caggcgttgt cgagatcgcg
      781 catcaacgcc gatccggacc agccattggt cacccgtttc gccgggcacc ccatattgat
      841 gtcgatgatg gctgccccgg cggcttcggc cgctctggcg ccttccgcca tccagcgcgg
      901 ctcgcagccc gcgatctgca cgacgtgggg cacgatgcca tcgccttccg cacgaagccg
      961 cgcctcctcc tcgccgagcg ccaactgatc gctcgcaacc atttcggaca cgaccagggt
     1021 ggcgccgaat cgaagcgccg cgcgccgcat tcccgcatcg gtgatgccgg acatcggcgc
     1081 aaggagcgcc ccgccaggcc gccgaagctg ctgcagaatg tcgccagatg cgggcatcga
     1141 gagcaatgtg cctatttttt aatcagacgg gtgacctgcc ctgaatatag tcattttttg
     1201 caatgcagca aagtccggaa ctgcacggcg ttgcgaccgg tcttggccag ggatggcgcc
     1261 ggattgaccc ggcgtgcggc gcggtccaag gaagggccat gaccgagcct gtcatcgccg
     1321 ccctgatcgt tgccgccgga cgcggatcgc gcatcggagg ctataccgcc aagcaatatc
     1381 tcaaagtcgg tggtcgcagc atcctggcgc gcgcgatcga tggctttgtc gcccacccca
     1441 tgatcgaacg gatccagatc gtcatccatc ctgacgatca ggatctcttc gataccgtac
     1501 tggctgagtt gacacctcgc cctgcgaagc cgatcgaggc ttgcggcggc ggcgcgtcac
     1561 ggcaggagtc cgcgttgctc ggcctgcggg cgctgaaggc gacgctcgca ggggacagta
     1621 cctgcgtcct tgtgcacgat gctgcgcgcc ccttcctgtc cgaggcgctg atcacccgtg
     1681 ccatcgctgc cgcctggcgg cacggcgccg ccattccggt cctgccggtg gtcgatacgg
     1741 tagcgatgct cgattccgct ggagacctgg ccggcaacgc ggatcgcggc gcacttcgac
     1801 ttgtccagac gccgcaggcc ttccgtttcg gtccgttgct ggtagcccat gaggcggcgg
     1861 cgcaacaggg acgcagcgac ttcacggacg acgccagcct tgtgcgctgg cgcggcgcta
     1921 ccgtcgcgac cttcgccggc gatcccgccg cgttcaagat caccacaccc gaggacctgg
     1981 cccgcgccga agcgctggcc ggcgcccgcc ttgccgacat ccggtgcgcg accggctatg
     2041 atgtgcatgc cttcgggccg ggcaactcca tcatgctggg tggggtcaag atcgcccatg
     2101 cccgcggtgt gatcggccat tccgatgcgg acgtgttgct gcacgccctg accgacgccc
     2161 tgctcggctg catcggcgat ggcgacatcg gtgtgcactt cccgccatcc gacatgcggt
     2221 ggaagggcgc ggcttcccgt gtctttctgg aggaggcggt tcgccgcgtg catgcccggg
     2281 gcggctcaat acgccatctc gacggcacca tcgtgtgcga agcaccgcgt atcggcccac
     2341 atcgcgaggc gatccgacag acggttgcgg ccattgcggg aatcgattgc aatcgtgttt
     2401 cgctcaaagc cacgacatcg gagaaactcg gcttcaccgg tcgcggtgaa gggatagccg
     2461 cgctcgccac agccacgatc gccctgccgg agtaaggagg ccgccatgcc tgacaccaag
     2521 tcgcccagcc atctgctcgc ggtcgaggtg ctgaaacttg cacgcgcccg gggcgagcgc
     2581 cttgtcacgg cggagtcctg taccggcggg atggtggccg ccgcattgac cgacatcgcc
     2641 gggtcgtccg atgcgttcga ccgcggctac gtcacgtact ccaacgacgc caagcaggcc
     2701 atgctgagcg tgccggagaa gctgctcaag ggctatggcg cggtttctgc tgaggtcgcg
     2761 gccgcaatgg cgctgggagc actccggcac tcgggcgcgc atctggccgt ggcgatcacc
     2821 ggaattgccg gaccgggcgg aggaaccgcc aagaagccgg tgggactggt gcatttcgcc
     2881 gtggtgcacc gcgacggtcg ggtcgatcgg gtcgaaaaac ggttcggcaa tctcgggcgc
     2941 gcggagatcc gggctcggtc ggtcgagaca agcctggaca tgcttcgcgc gatgctggcc
     3001 gcgccggctg tggcctgacg cgggaagcgc tggtcggccg ggcgctactg ccaggccttg
     3061 cgccacatgg ccccgacgat gttggagtaa tcatcggtcc atgccgtgac gccgccttcg
     3121 cgcagattgc gccagccata gtcttcaagc gccctgagcg cttccggcga gtgggacaga
     3181 gcgatgacgt gggagccggc cttgccgctg gccagatgct catccttctg gacggcgtca
     3241 agcttggccc gcccctcaac gccaaggtca ctggcgatgg ccgcaaccac gctctccaga
     3301 tcgagatagc gattggagat gtgcaggacc aaaaggccat cggtcttgat tttcgaaaga
     3361 tacatctgca ccgcttcccg ggtcaggagg tgcaccggga ttgcatcgga agcgaaggca
     3421 tcgatcagca gcgcgtcgta gggcttgttt tccttggcga gggtcagccg ggcatcgccc
     3481 agaaccatgc gtgcctcggg cgcacagacg gagaggaacc ggaagtagcc cggatccgtc
     3541 gcgatgcgca cgacggccgg atcgatctcg aaataggtcc actgatcgtt gttgcgcccg
     3601 tggcagacga ttgagcctgc gccgagtccg accacggcga aggagcgccc tgccccgttc
     3661 gcccgcgtct ggacggaacg gatcgattcc gccatcgggc cggaccttgc gtagtaggcc
     3721 agcggttccg gccggaacgg cagtggcgca ccttccgggg acttaatgcg catcgagccg
     3781 tgcaacgtcg tgccatgggc gagattgcgg tactggccat cattggtgac ctgaaccttg
     3841 tgaacgccga agaacgagcg cagcgtgagt tcgtcgcggg tgtttagcga aaacgatccg
     3901 atcaggggga aaacgccggc gcagatcgcg ccgacaacca gcggacggcg atacaggacg
     3961 agcgcgattc caatcgtcgc cagcagcagg gacaccagca ggttcttggc aatgcccgta
     4021 aagagctgcg gctgcttggc gacgacaagc tgcacaagca tcaggccgat cagccaaggc
     4081 gccgcctcgc gcacccactg cggccgcgac gcggcataga cagccggcag gcacaggaac
     4141 gtcgcgatga tcaggatcgg gtattccgcg atggagttga acacgttggg cgcgatcaat
     4201 ccgcagaaca ggccgcccag gacgccaccg aacgacatgc agagatagaa ggtcgtcagg
     4261 tgttgcgggt ttggccgcag ggcatagagc cgggcatgac agatcagggt cgccagcgtc
     4321 atgaagccga aatgcacgag catggacgcc cagagcatct ccgcgccgag gaacaggaac
     4381 agggcgagtg acgtcgccac gatcgggaga atgcggtcca gcgatccgat gtcaaacggc
     4441 atcggatcgc ggaagaccac cacgaaggtg gcgaggtaga gcgccagggg aatcacccac
     4501 aggaagggcg aagcggcgac atcggtggac acatgcgcgg tgaccgcgac cagaaggccc
     4561 gagggcacga aggccaatcc gatccactgt gccatgcgcg cccgggttgg cgcgggcgaa
     4621 tagtcgacga tgtcggcggt tcccctctgc gccttcgcat cgagattgcg gctgaccagc
     4681 acgcccgcga ggccgatcag ggcgcccagc acgcagaacc cgaaggacca ggcgtaggtc
     4741 tgtcccttga gcgtcagcag cggctcgatc agaacgggat acagcaagag cgcggcaaag
     4801 gacccgagat ttgacgcgcc atagagataa tacggatcct gcgccgtcgg atgtccgctg
     4861 cgcgcaaacc aggcctgaag caaggggccg ttggcggaaa ccgcgaagaa aggcaggcca
     4921 acggaggcgc agaagacacc gatgagccag atctcctgcc attgggtcgg cggctccccg
     4981 agactctggc tgacgccgat gggcagggcg aaggccgtca cgccgaacag gaccagatgg
     5041 gccagcgtcg cctgacgcat ggtgagatac ttcgtggaaa catgcgcata ggcgtagccg
     5101 gcgagcagaa gcgcctggaa gaacaccatc gccacggacc agacgccggg cgatccgccc
     5161 agcagcggca ggaccatctt cgtgaacatc ggctgcacgg cgaacagcag gaacgccgat
     5221 gtgaaaatcg ccgagataaa ggccgccagg accacgccat gagaagccgt gccgcgacgg
     5281 ccatgggcgg atgcgtagac gggatcgatg gaggtcatgc gcgcattcca gatgaaattg
     5341 cgcgcagatt atctctggag gcttaaggct gctttaagcc tgtgcgcgtc gagacctttc
     5401 cagtgcgtcg gcgcgttcct cgaaggattc agccatcttt cggaaaagct tgtcgaaaac
     5461 cgcgccggcc agaatctcga aagcacgact gcggaaggag tagtcaatcg agaaatcgac
     5521 cttcgagccg ccctcgccgg aatcctcgaa caaccagcgg ttttccagat gtcggaacgg
     5581 tccgtcgatg tattccacca gaattcgtcg tcgcgcgcga tccaccgtga cgcggctggt
     5641 gaagctttcg tggatcatct tgtagccgat ggtcatgcgc gcggtcagga tgtggcgacc
     5701 gtccggcgcg tcgacgcgac gcagcacctc cagctcctga caatacggca ggaactcggg
     5761 atagcgctcc acatccgcca caagctccag catggcggtg gcggtatggg ggacgcggcg
     5821 cgtggtcgaa aagtgcggca tggcggcgcc gccttctggc atggaccggc gcgcggttca
     5881 acgctcgcga agcttggcga ggaatgcgcc caacggattg gcgtagtcgt ccgtccaagg
     5941 tctcggcaat cggacgccag gggcctgcca gtcgtcattg tcgttgagca cgccgaagtg
     6001 ctcgctcttg cgggcgagta ccgccacggt cggctgtatg gtcatcgcag gggctccgtc
     6061 gccctgctcc tcgtcgttga tccgtaccgc aagttcgagt gacagcgccg tggccgcgac
     6121 cacctcctgc aggcgcaggt gattgctgga gatatggagg agcagcgtac cttgcggagc
     6181 cagccgcaca acaaaggacg acagcgcctc gcgcgtcagc aggtgagccg gtatcgaatc
     6241 ggacgaaaat gcatcgagga tcaggatgtc ataggttgcg gcttcccgtt ccaacgtcag
     6301 tcgggcatcc cctgcgatga ttgccgcgtt cggggcgcag tcggacagaa agcggaactt
     6361 cgtccggtcg cgggcgatgc cgatcacaac gggatcgatt tcgtagaagt gcagcgtatc
     6421 actcggctcc accaggcagg cgatcgagcc ggcgccgagg ccgacaacgc cgacacgcag
     6481 cgcaccgccg ccggccttgg tccgcgcggc gtcgatggct tcggaaatcg gcgagtcctc
     6541 ggaatagtag gtcagcggca ccggcttgcc acgcaccggc cgaccggccc tgtccgcgat
     6601 ctgttgcgcg ccgtgcactt caacgccatg gcgcaagctg cggaactggc cgtccgccgt
     6661 cgtttcgatg gtgtggacgc catagaagct gcgaagcgtt tcgcgttcac gcacttccag
     6721 gggaaccatg cggccgaaca gcagcaccgc cacgaggatc gccgccagcg ctggaccgaa
     6781 acgcggcagg gaaaacgccg ccagtgccaa tgcgatcggg tattccgcga cgaacgtgaa
     6841 caggcttggc gctgccagcg cgcagagcgc accgccaaga accccgccgg acgacatcga
     6901 aagatagaag ccggtcagcc ccccggggtc cggacggcat gccgccaggc gcccgtgact
     6961 gtagagcgcc agcagaaaaa acacggtcag atgcaagagg acatccagaa gtggccaggt
     7021 ccgcacttcg aaggtcagca gcagggccag cacaagcagc gacggcccca gaaccttccc
     7081 gagcttgtcc agccatgccg tgccggagac gccaaaggcg atgatgaagg tcgcgagata
     7141 gagcgccagc ggcgccaccc acagaaacgg cgcggcaaca atatcggtcg aaagatgcag
     7201 cgtcaccgcg accagagccg ccgaaggcgt cagggccagc ccggtccagc gcaaaatctc
     7261 gctccaggaa gcttcccggt gcggtctggc ccgccggatc ggaagcgccg gcgcctttaa
     7321 catggcgaga gccgccagca gaagcagcag gagcagcgcg ccatagccga cgctccagcc
     7381 cagggcttgc tggcgaaggg tcagcagcgg ttcgaggaac gatggatagg ccagaagcgc
     7441 cgcaaggctg ccggcgttgc ttacggcata gagcggatac gggttgccgg cgcgcggcgc
     7501 tccggagttt gcgaagcaga tttgcagcaa aggtgcgttg gccgatagag cgacgaaggg
     7561 caccccgatc gagatggtca gcagaaccaa caggcgcagg ctttcctcat tcccgaacgc
     7621 agcctcgaat cggggctgga gcggcaagaa cgccaggcca agcgccagca ccaccgcatg
     7681 cagcaccaac gcaaatcgca aaggcacgaa gcgggcgaga acatgcgcgt aggcatatcc
     7741 cgcgaggagc atggcctgaa agaacaccat ggcgacagac cagaccgacg cggaaccgcc
     7801 gaaccggggc agcaccatgc gcgtgaacat cggctggatg gcaaaaagca gcgccgcact
     7861 cagcccggag gccgctatga agagcagcag cacggccgca tccgcccgga ctgcgcgccg
     7921 cgccaagggg gaaaaagaca ggagcctgtc gatcacgccg cactccagac accgccaccc
     7981 ccgcgactca gaatacggga cccgggccag cacaacaaca agccgaacat gaaatcttgg
     8041 caatacttgg cagatcgcac gccgagtgtg ggaatcgttg ccgttccggg caaggacatg
     8101 gaattatcgc ggtcggtcga acgtcctcaa ccgccaaaaa ccctgctcaa ctcctcgacc
     8161 tgtgcttgcg tgagcctgcg ggtggggtga ccgcgtagca gaaggaccag ccgcgccgtt
     8221 tcctccagct cctctgccgc gaacacagcg gactccaggt ccctggccga gacgaccggg
     8281 ccatgattgg ccagaaggac ggcggcatgc cgaccctcca gcgcccggat catatccccc
     8341 atcgccggat cgccgggcct tacatagggc agcagtctga cccgtccgac gcgcatcacg
     8401 acatacggcg tcagcggtgg aatgcagtcg tcggcatcgc tttcggcgag gcaggatatg
     8461 gctgtcgcgt aggtcgaatg gagatggaca acggccccgg ctgccggccg ggtttcatag
     8521 aacgcccgat ggagaaaaac ttccttcgac ggcttgtcgc ccgccagatg tcggccgccg
     8581 gcatcgagtt tggaaatgcg tgccggatcg agaaagccga gacaggaatt ggtcggcgtg
     8641 atcagcatgc catcatcgag gcgagcgctg atgttgcccg acgagcctac ggtgaagccg
     8701 cgctcgaaca gggatttcgc cagccgaacc atggagtcgc gcagggtgga ttccgcgctc
     8761 acgcttcgcc ccccagcgta gccagcgcct tcgcgaagaa atcgaccgcg ccgaagtttc
     8821 cggacttcag cgccacagcc agaggcacgc cggatgcgtt ggacaaggcg ggcacacccg
     8881 gatcaatctc cgggcctatc gccagggcct ccaggccgag tgcggagacg acagcgcccg
     8941 aggtctcgcc ccccgcgacc accagccggc ggataccgcc atcaacggcg gcgcgggccg
     9001 tttctgcaaa cagggaatcg agcctgtgcg ccactgcctc ccgcccgaaa cgcgcttgca
     9061 gcgcagcgac ttcatccggc ggcgacgacg agaagacgag cggtgcgcgg cccgcgttgc
     9121 gaacgaggaa accgacaagg tccgccatca ccacggcgcc actcatgaca ccggcgacgt
     9181 cgatgggcag aacgggatgg ccctgggcct tgtggtgcgc gatctggccc agggtcgcgc
     9241 gggaacagga acccgcgaga atggcttctg gcccatccac gcggcgaaag gcggatgccg
     9301 cccctttggc caaccccttc cggatgaaat tgcgcgacag cccgagtgcg acgcctgaac
     9361 cgccggtcag caggggtgca tcggccacgg cctcgccaat ggtcagcaga tcgtcgtcgg
     9421 aaatggcatc gacgatgacc agggtctccc cgcgccccgc cgcctccgca agcgcgtcct
     9481 gcagcgcggc cgcccctttc gccacggtcg cattccagga cgcgatcccg accggcgtct
     9541 cagtctgccg gcgcagccat cggcggatgt cggcgtcggt catgggattg aggggatgat
     9601 tctccagccc agactgactg agcagccgat ccccaacgaa cagatggcct tgatagaccg
     9661 ttcgccccgc gccgggaaat gccgggcagg cgacaacccc gtggacgccg agcgcacgcg
     9721 ccagggcttc ggcgaccggg ccaatgttgc cgtcgggcgt cgaatcgaag gtcgagcagt
     9781 acttgaatac gatctggcgg cagccctggg cctggagcca gcgcagtgcc gcaagtgact
     9841 gcgcaacggc gtcgcctgcc gggatggagc ggctcttcag ggcgacgacg ccggcttcgc
     9901 agctttcggg cgccctgccc ttcgggatgc cgaggaactg cacagtcgcg aggcccggcg
     9961 tgccaccgcc acccttggcg atcgtgttcg cgatgtcgga tgcgccggta tagtcgtcgg
    10021 cgatcacgcc cagaagcatg tgcagggttc ccgtttccgc tgtcggttgc ggtgatgcta
    10081 gcatcgtccg gcacggcaac gctacggcgg atcgccgggc cggcgcgtgc gcccgcaact
    10141 gaaaggctct catggtttgg cggaaggggt gggattcgaa cccacggtgg gcttgcaccc
    10201 acgccggttt tcaagaccgg tgccttaaac cgctcggcca cccttccagc gcgccgagca
    10261 acagcaccaa tcgcggaaat ccgcaagacc agaacgcagc ctcaagcgct agcgaccaaa
    10321 cacgctgtag ggcagcgtgt cagtggcgat caggtaaagc agtcccgtca cggtcagcgt
    10381 cgagaccaga gtgcccacga ggatgccggt cgaggcgcgc tcgacataga catcatactg
    10441 gcgtgcgagg acgaaaacat tgagcgctgg cggcagcgcc gccatcagca ctgcggcaaa
    10501 gacccactcg cgttcgaaac cgccaatgag cgacagcagc acgagcacga tgaggggatg
    10561 cacgacgagc ttgaccagca ggtgtgccgg cacctcgaac gggacactgg tcaaggggcg
    10621 aagcgccacg gtgacgccca gagtgaacag cgcgaccggc gccgcggcgt tcttgagata
    10681 ggttatcatc gtgtcgaccg gtgtcggcac cttgatctgg aagtaggcgg ccaggaccgc
    10741 cacgaacgtc gcgatgttga agggatggga gacgacccgc caaatcacca gcttggtggt
    10801 ttcccacacg ttcaatcgtc cgctcgaccc catcgccatc aggaagggca cgagcgtgaa
    10861 caacagcgcg ttgtcgaaca cgaagatcag cgccgtagga acggctgcct tggacccgag
    10921 cgcggcgagt gtgaggcctg gacccatgta gccgatgttg gaataacttc ccaggacggc
    10981 ctggatcgtg gctgggcgga ggtcgcctcg cgaagcccac agcccgatgg ccagcgagat
    11041 cacgaatgcg agataggtcg agagcgtcgt gccgatgacg aagggccagt tcttcaactc
    11101 ttcgaacgga gtcgcggcga tcagcttgaa gaacaggggc ggcagcgcga catagacgat
    11161 gaaaaagttc atccagcgca agccttcttc gggaaatcgc ataagctttc cgcagaaaaa
    11221 gcctagcaaa atcagaccga agaagggaaa tgctaaagtt aggacttcgg ccatgagctc
    11281 aatgcaaggc gtcgtcggga gacgcgctgt gaggtgttga gaggcctcga aatccgccga
    11341 accgacagca tcgggcgtgt tccgatagca acgacggacg gccccgtcca gaccggtccg
    11401 tcgttgcggc ttcccctttt cgcgatccgg ccatatgtta cggagccgga aatgaccatt
    11461 gacgtccgct tgccggaccc ctaaacggcc gcgctttcgc tgggagcatc gttcaatgcc
    11521 tgcgcgcgac gcaaaatacc ggattggaca ggtggtgcgc catcgcatct acccgttccg
    11581 gggtgtcgtg ttcgacatcg atcctgaatt cgccaacacg gaggaatggt ggcagtccat
    11641 tccaccggag gtccggccgc ggaaagacca gccgttctat cacctctatg ccgagaacga
    11701 cgagaccgag tacgtcgcgt atgtctcgga acagaacctg attccggata cgtcgaagga
    11761 gccgctgcgc catccccagg tgcgcgaggt gttcgtcaag aaccgggatg gttcgttccg
    11821 gatgaagtct ggcgtgtttc actgacgaga accgctcgtc gcgtctggcc ggcccggtca
    11881 gacgcccgcg gacgcccaaa acaaaaaccc ggccgaagcc gggttccgcc atttatatcg
    11941 gctgctcgcc cctactgctt tggcgcttcg ccctgcttct tgcgcagttc ttccgcttcc
    12001 ttctgcaggg cttcctccag catcttgcgc tgctgttcca gcaccttcgg atcgatcggc
    12061 gcgccatcga acgccttgcc gaatccggcg agcggcacca caaaggcaat ctcctgtccg
    12121 cgatcgttct ggacgctgac cttgacgttc gtgcccttct tcaggttcgc gaggaacgcc
    12181 tcgttcactt cagcctcggc gaagcagccg ttggggaagc agatctggaa ccggccggaa
    12241 accggctgag ccgaatccac gccgaagcgg atgccaggct gcagcaggaa cgacagcggc
    12301 aggaggaaac ggacgaactt cttggggtcg ttctgcacgt catagacggc gacagccatg
    12361 atcggctggt tgttgtccga aaggaagtcg cgcgtcgtgt agcagatggt cttcttggcg
    12421 gccggatcct cgccgcagac cttcgtccac tcctgcgccg catcggcctt cagattgacg
    12481 agcgtggggc ctgcaggcgc ggcgggctgc tgtccctgcg gggcagccgg agc
//
LOCUS       DAOMXN010000048        12511 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_2741_length_12511_cov_12.029383, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000048 DAOMXN010000000
VERSION     DAOMXN010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 12511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12511
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2741_length_12511_cov_12.029383"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..275)
                     /locus_tag="PK812_05610"
     CDS             complement(36..275)
                     /locus_tag="PK812_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HRJ69068.1"
                     /translation="MAGHAIPHFHNDTGAPTIEIGVKEFMCIGAKPPFDHPHVFIDMG
                     AENEAICPYCSTLYRYSKDLHGEESRPPAAWHEAA"
     gene            389..1156
                     /locus_tag="PK812_05615"
     CDS             389..1156
                     /locus_tag="PK812_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRJ69069.1"
                     /translation="MQRFVHKGVGIAFIDVPPHQGDGPVVLAIHGFASNHSVNWVGPQ
                     WVRSLTHAGYRVIAMDNRGHGQSDKLYDAAAYDTRLMADDAAGLLEHLDVRTAGVIGY
                     SMGARIAAFLAVLRPDLVASLALGGLGIHLIDGVGLPQSIAAAMEAERLEDVADPMGR
                     MFRAFAMSTKSDLKALAACIRGSRQVLTPRETGQILAPTIICVGTNDPIAGDPHALAR
                     HIPGAKAFDIEGRDHNLAVGDRTHREAVIKHFNETLK"
     gene            complement(1246..2046)
                     /locus_tag="PK812_05620"
     CDS             complement(1246..2046)
                     /locus_tag="PK812_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRJ69070.1"
                     /translation="MRQLPSPCPRLTASVDGPIGRVTIANAAKKNAIDIAMWRAFPPL
                     LEALAADPEVRLIVLAGQGANFSTGADIAEFDTERATPEGSRAYEVENVRAFEAVSLC
                     PRPTVAAIRGFCFGAGAGLALSCDLRIAADDATFAIPAARLGVAYPPAAFANIVAAIG
                     APRTKEMFFTGQRIDAASALSAGLLNRVVASVDFEGALASLAQAIAAGAPMTLTAAKR
                     AIDAAAGLPGALSATTVQSLADACFDSADYAEGRAAFREKRPPQFRGR"
     gene            2141..2962
                     /gene="cysE"
                     /locus_tag="PK812_05625"
     CDS             2141..2962
                     /gene="cysE"
                     /locus_tag="PK812_05625"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HRJ69071.1"
                     /translation="MAHTRNEAPKRLEAVDPVWTRLRREAEDIMRAEPSLSGLVLSNI
                     LHQPNLHLAVAQRVSSRLDHPDVTGALIFQAFGELHQAQPELAAWLRTDIMAVLDRDP
                     ACNRVIEPVLFFKGFQAIQTHRLAHWLWMNGRKDFALYLQSRSSTIFQVDINPAARLG
                     KGVFFDHATGIVIGETAVIEDDVSILQGVTLGGTGKETGDRHPKIGRGVMIGAGAKIL
                     GNIEVGHCARIAAGSVVLQPVPHNKTVAGVPARIVGEAGCAEPSRAMDQLLHEAG"
     gene            3038..3247
                     /locus_tag="PK812_05630"
     CDS             3038..3247
                     /locus_tag="PK812_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3126 family protein"
                     /protein_id="HRJ69072.1"
                     /translation="MEKPDIQRVENYLRRTFNNASIKVLARAKKKDSAEVFIGDEFIG
                     LIFEDKEDGDLSYNFQMAILDTDLD"
     gene            complement(3302..4009)
                     /locus_tag="PK812_05635"
     CDS             complement(3302..4009)
                     /locus_tag="PK812_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69073.1"
                     /translation="MPTGIESRSVEQKRGGAVRVMLRIAVLAVLLVIAAAAGLLARRI
                     YVERTPPAGAAEFFIGSQKVLVPRAMVRDPDLRNGGALNRIDLALLWPDLRGAAAATK
                     AEIDRGMVFIGLEDAAPRAATPGDIDPTDRPGELYARFLAQEAWSNPGGLVMRRFRAG
                     TPYEGEELFVSSPDERVFSARCPIRKLGNTTEDLCLWQTRITGMELQVRFSPRLLVEW
                     QALSGGVRALVTRLKAR"
     gene            4077..4397
                     /locus_tag="PK812_05640"
     CDS             4077..4397
                     /locus_tag="PK812_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69074.1"
                     /translation="MSAFQLEYVLMSAAGMAFAGVLAEAFHLISGCRVSFSLFGQGRG
                     RAWLAAPLLFATGPFVLLRNNYRAYVLEGRPLRFVLAGAAVATVWALLTGFLLLGLAE
                     WASG"
     gene            complement(4507..5136)
                     /locus_tag="PK812_05645"
     CDS             complement(4507..5136)
                     /locus_tag="PK812_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like cysteine peptidase"
                     /protein_id="HRJ69075.1"
                     /translation="MLGNGLTQISRIVFAATLLTCIGGQFATAQQLNSNIAPAGQQTS
                     PPIGWVEFCEREPSDCNVPQTRPSIVSLNEGNWREILRINAAVNREIAPITDMEHHGV
                     VEHWSYPTEGKGDCEDYVLEKRKRLIRAGFPRQALLITVVRDHKGDGHAVLTVKTDRG
                     DFILDNQAPKVLAWSETGYRFIKRQAQDNPNRWVSLGGYDTSTVAGTRN"
     gene            complement(5750..6346)
                     /locus_tag="PK812_05650"
     CDS             complement(5750..6346)
                     /locus_tag="PK812_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRJ69076.1"
                     /translation="MLVPNSRISVRPVDLRRFQRVEVALHGRYMLPDRTEHECMTVDM
                     SPGGVRLRCTAAGNVNDRVVAYLETVGRIEGNIARRTPDGFAMTITATLRRRDKLAAQ
                     LTWLANRSELGLPEDRRHERHVPRNPHARLVTASGSEIMVRLIDVSLSGAAFNTDLPF
                     NKGEMILLNQTPAKVVRVFERGIAVEFTRAPAPEAFQV"
     gene            complement(6476..7072)
                     /locus_tag="PK812_05655"
     CDS             complement(6476..7072)
                     /locus_tag="PK812_05655"
                     /inference="COORDINATES: protein motif:HMM:NF018960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRJ69077.1"
                     /translation="MRFESSKHIFAYWRRLRAGATAPERNAIDPVALGRHLADVFLLE
                     RDAEGAFRFRLAGSSLCVVFGQELRGREFLSVLTQAAADDGGEILLTATEDQFPVVAG
                     VSVLMADRRSFDAELLVLPLLQNGRAGVMLFGSLAYKRQDRFALGTGAGLEILSFRVI
                     GERDTRHLHPPTAGVVIDGHKGERRGHLVVFNGGSPGF"
     gene            7466..7897
                     /locus_tag="PK812_05660"
     CDS             7466..7897
                     /locus_tag="PK812_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012454115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRJ69078.1"
                     /translation="MIVSHILDQKGRDVATTRPHLTMMDVVRHLGDRRIGALVVTGAD
                     GAILGIVSERDVVKAMAKGGAAALEDPVSRHMTAKVVTCAPEDHADTVMEAMTAGKFR
                     HMPVEAGGKLAGIISIGDVVKARIEQMASESQALRDYITMS"
     gene            complement(7908..8942)
                     /locus_tag="PK812_05665"
     CDS             complement(7908..8942)
                     /locus_tag="PK812_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009489444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRJ69079.1"
                     /translation="MNPTELLEEALLAVLNFRRDPQPPAEEQEQPVSRPQPPVIALAL
                     GAGAARGWAHIGILRVLEREGLRPKIITGTSIGAVVGGCWSAGGLDQLEIFARRLTKR
                     GVFSLLDLNLSRTGLMGGRRLRDMLEDGLKDRTIENLPTRFAAVTTEFGSGHEVWLTR
                     GHLVTAMRASYAIPGVFEPVRVGGKWLLDGAMVNPIPITTARALGADLVIAVNVNTET
                     FGRGTVIADHHASPPPVPESAAAEGGFLKPMRNAAHAVTGYFNPRSNSESPPSIAAVM
                     VETINITQDRIARSRLAGDPPDVMIAPKMAKIGLFEFHRADEAIAIGSEAAERMLPEI
                     REAYAALRGA"
     gene            complement(9005..9430)
                     /gene="hisI"
                     /locus_tag="PK812_05670"
     CDS             complement(9005..9430)
                     /gene="hisI"
                     /locus_tag="PK812_05670"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007567355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HRJ69080.1"
                     /translation="MWTRATPFAPAGTKLDTEEGQRLTPTFDANGLIPAIAQDASSGE
                     VLMLAWMNAEALARTIDTGEAWYWSRSRRKFWRKGESSGHVQQVVEISIDCDQDAILL
                     KVQQTGPACHTSRRSCFYRSVRRADDGSLTLIFNGSSGA"
     gene            9508..9957
                     /locus_tag="PK812_05675"
     CDS             9508..9957
                     /locus_tag="PK812_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HRJ69081.1"
                     /translation="MLNDVYNQRILELAADIPRLGRLPQPQATATAHSKLCGSTVTVD
                     LCLNDGRVSAFAHDVKACALGQAASSIMARNIIGSTPDELRAIRETVRRMLKENGTAP
                     SDGRWSDLRVLEPVRDYKARHASTLLTFDAVCEALGKIEAQAAAQQQ"
     gene            complement(9973..11265)
                     /locus_tag="PK812_05680"
     CDS             complement(9973..11265)
                     /locus_tag="PK812_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69082.1"
                     /translation="MLLALSIAAATAAATNAAHAQQRRAPASAEGAPDRMVFELATND
                     PEAPRDKQIRWVSARGGIVRDTPKDFDEFLKKHDITGLTIYFDSSGGSINGGLELGER
                     LRKLSAKVSIGRSLPVGTAEMVGSGDAPPLRRHQLSPNSGQCNSSCAYAFLGGVKRTI
                     PASATYGVHMFWPSDKAEKLHQQNYVSRDIENAQRTASRIVVYLQKMDVDLRLFELAS
                     TTPHKGQIRRLTPKEITDLRVASIEHATPLLAREGNWGLLVGRSTATLVTGGPLPGAE
                     RAGANFQLEFTCNATRGFHNARLELSVSKSLPEDRPVALRRVLLVSGTKDAVIAISGK
                     DIRAMPATFNRLTARNPGAWIAKAGTVVSEVVENAVTRPSDGLSLRIDEGNDSVYSLK
                     LALGNLPEQYRPWVAACDGFRARASSEPEPRGAGQPER"
     gene            complement(11339..12154)
                     /locus_tag="PK812_05685"
     CDS             complement(11339..12154)
                     /locus_tag="PK812_05685"
                     /inference="COORDINATES: protein motif:HMM:NF018352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HRJ69083.1"
                     /translation="MFANRSIACAFAMTVAGSAMAADKPTPYTPTPASGWIVTVKANA
                     VSGPKWDGADTNGFIAYPSLSFRRAGTKPKWSSPDDGIGFSLVDTGPFEMGPVARIRS
                     GRYNGGDWRLAGIHDVKWTVEPGVFANFWITPNLRARAEARHGFRSQDGFHANFGLDW
                     VQAFDRFTFAIGPRLEVADSKFMRTNFGVTALDASRNALVWTYNAGGGVKSYGVYSSL
                     GYQFNDSWSATLHGGYNRLANEPGNSPIVKRIGSSNQFIVGLQLAYSFPISGF"
     gene            12290..>12511
                     /gene="yidD"
                     /locus_tag="PK812_05690"
     CDS             12290..>12511
                     /gene="yidD"
                     /locus_tag="PK812_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HRJ69084.1"
                     /translation="MKTSLLSLPGRAASLALIGLIRLYRYTLSAFMGRTCRYLPTCSE
                     YTEEAIRRHGAWAGGWIGFARICRCRPGGA"
BASE COUNT         2256 a         3841 c         4078 g         2336 t
ORIGIN      
        1 actgccgatt tgcccccgct cacggcatca ggccctcagg ccgcttcgtg ccaggctgcc
       61 gggggacgcg actcttcacc gtgcaagtcc ttgctgtagc ggtacagcgt ggagcaatag
      121 gggcagattg cctcgttctc cgcacccatg tcgatgaaga cgtgggggtg gtcgaagggc
      181 ggcttggcac caatgcacat gaattccttc acgccgattt cgatcgtagg cgcaccggtg
      241 tcgttgtgaa aatgggggat tgcgtgacca gccatggaat cctgctttct tgcaagcgca
      301 tgtcctaaac cctcgatacg gaaattccta ggcccttgac ctcagacatt cgatcggctt
      361 tgcgccttgg cgaccgcagg atggtgtgat gcagcgtttt gtccacaagg gtgtcgggat
      421 tgccttcatt gacgtgccgc cgcatcaggg cgacggtccg gtggtgctgg ccatccacgg
      481 ctttgcctca aaccactcgg tgaactgggt ggggccgcag tgggtgcgta gcctgaccca
      541 cgccggctat cgcgtgatcg ccatggacaa tcgcgggcat gggcagagcg acaagctcta
      601 cgatgcggcg gcctatgaca cgcggttgat ggctgacgat gccgcggggc tgctggagca
      661 cctcgatgtc cgcaccgccg gcgttatcgg ctactcgatg ggggcgcgca ttgctgcatt
      721 cctcgccgtg ctgcggcccg accttgtcgc ctctctggcg ctcgggggac tgggcatcca
      781 tctcatcgat ggtgtcggcc tgcctcagag tatcgccgcg gccatggagg cggaacgcct
      841 tgaggatgtc gcagacccca tggggcggat gttccgcgcc ttcgccatga gcacgaagag
      901 cgacctcaaa gcgctcgccg cctgcatccg cggttcccgt caggtcctga ccccgcgtga
      961 gaccggccag attctcgcgc cgaccatcat ttgcgtcggc acgaatgacc cgatcgcggg
     1021 cgatccccat gcgctggcca ggcacattcc gggcgcgaag gccttcgaca tcgaagggcg
     1081 cgaccataat ctcgcggtgg gcgatcgcac gcatcgcgag gcggtgatca agcactttaa
     1141 cgagacgttg aaataggttt tatttcaagg agataatctc gcgtttcgag aatctgattc
     1201 cggcaattca cgtggtgaat cacgcgatgt gatgtgccga agcctctatc gccccctgaa
     1261 ctgcggaggg cgcttctccc ggaaagccgc ccggccttcc gcgtagtccg cgctgtcgaa
     1321 acaggcgtcc gccagcgatt gcaccgtagt ggctgacaag gcgccgggca ggccggcagc
     1381 ggcgtcgatg gcgcgctttg cggctgtcag ggtcatcggc gcgcctgccg caatcgcctg
     1441 ggcaagcgag gccagcgcgc cctcgaagtc gacgctggcg acgacacggt tcagcaggcc
     1501 cgcggacagc gccgacgcgg catcgatgcg ctggccggtg aagaacatct ccttggtccg
     1561 gggtgcaccg atggccgcga ctatgttggc gaaggccgca ggcggatagg cgacgccgag
     1621 tcgtgccgct ggtatcgcga atgtcgcgtc atccgcagcg atgcgcaggt cacaggatag
     1681 tgccagcccg gcgccagcac cgaagcagaa tccccgtatg gcggcgaccg tcggtcgcgg
     1741 acagagcgac accgcctcga acgcgcggac gttctcgacc tcgtaggcgc ggctgccctc
     1801 gggcgtggcg cgttcggtgt cgaactcggc gatgtccgcg cccgtggaaa agttcgcgcc
     1861 ctgacctgcc aggacgatga gccggacctc gggatcggcg gccagcgcct ccaggagcgg
     1921 cgggaaggca cgccacatgg cgatgtctat ggcgtttttc tttgccgcat tggcgatggt
     1981 cacgcgtccg atcggcccgt cgactgaagc ggtcaagcgg gggcagggcg agggcaactg
     2041 gcgcatgctg tcaacttttc cgtattaggc ctcgcgaaag gcattgtgcg gcactatgtc
     2101 ttgagccatc gcgtgacaag caggaatggc ggagggaccc atggcccata cgcggaacga
     2161 ggcgcccaag cgtctggaag cggtcgatcc ggtctggacg cggctccgcc gcgaggccga
     2221 agacatcatg cgcgcggaac cttccctctc cggccttgtt ctgtcgaaca tcctgcatca
     2281 gccgaacctg caccttgccg tggcacagcg ggtgtcgtcg cggcttgacc atccggatgt
     2341 cactggggcg ctgatcttcc aggcgttcgg cgaactccac caggcgcagc ccgaactggc
     2401 cgcctggttg cgcacggaca tcatggctgt gctggatcgc gatccggcat gcaaccgggt
     2461 gatagaaccg gtgctgttct tcaaagggtt ccaggccatt cagacgcatc gcctggcaca
     2521 ctggctgtgg atgaacggtc ggaaggattt cgccctgtat ctccagagcc gctcgtccac
     2581 gatcttccag gtggacatca atccggcggc gcggcttggc aagggcgtgt tcttcgacca
     2641 cgcgaccggg atcgtcatcg gcgagacggc ggtcatcgag gacgacgtgt cgatccttca
     2701 gggtgtgacg ctcgggggga caggcaagga gacaggggac cgccatccca agatcgggcg
     2761 cggggtgatg atcggggccg gcgcaaagat cctcggcaac atcgaggttg gtcattgcgc
     2821 gcgcatcgcg gcgggctccg tcgtgttgca gccggtgccg cacaacaaga cggtggccgg
     2881 cgtgccggcg cggatcgttg gcgaggccgg ttgcgccgag ccttcgcgcg ccatggatca
     2941 gttgctgcac gaggcaggct gaccgcttgc tctcgcctgc cgctcgtggc aaggctctcc
     3001 cagtcagcag accgcatcgc gggttgggag aagtgtcttg gagaagccgg atattcagcg
     3061 cgtcgaaaac tacctccggc gcaccttcaa caatgccagc atcaaggttc tggcacgggc
     3121 gaagaagaag gattccgccg aggtcttcat cggtgacgag ttcatcggcc tgatcttcga
     3181 ggacaaggaa gacggcgacc tgtcctacaa tttccagatg gcgatcctcg ataccgatct
     3241 ggattgacac tgaaggcgac ggcgatcgat cctgcctgtc gcgccgcgct acgagcgacc
     3301 ttcaccgcgc cttcaggcga gtgaccagcg cacgaacgcc acccgacagg gcctgccact
     3361 cgaccagaag ccgaggcgag aaccgaacct ggagttccat gccggtgatt cgggtctgcc
     3421 aaaggcacag gtcttcggtg gtgttgccga gtttccggat gggacagcgc gcggagaaga
     3481 cgcgctcgtc cggcgaagat acgaaaagct cctcaccttc gtatggtgtg ccagcacgga
     3541 agcgtcgcat cacgagtccg cccgggttgg accaggcttc ctgcgccaga aagcgcgcat
     3601 agagctcccc agggcgatcg gtggggtcga tgtcgccggg cgtggccgcg cgcggggctg
     3661 catcctccag gccaatgaac accatgcccc tgtcgatctc ggctttcgtc gcagcggccg
     3721 cgccgcgcaa gtccggccac aacaaggcta ggtcgatacg gttcagcgca ccgccattgc
     3781 gcaggtcagg atcgcgcacc atcgcgcgtg gaacgagaac tttctggctg ccgatgaaga
     3841 actcggcggc gcccgcggga ggcgtgcgct ccacgtagat ccttctcgcg agcagtcctg
     3901 cggctgcggc aatcaccagt agcacagcca aaacggcgat acgcagcatg acccggacag
     3961 cgccccctcg cttctgctcc acgctacgcg attcgatgcc ggtcggcatg aagctcgcta
     4021 ggggcggagt gttgccgcaa cctccaggcg gcgttgttcg tgaacgggac gaaagcatgt
     4081 ccgcttttca actcgaatat gtgttgatgt cagccgccgg catggcgttc gccggcgtac
     4141 tggccgaggc gtttcacctg atttcaggct gccgggtcag cttctcgctg ttcgggcagg
     4201 gtcggggacg cgcctggctc gccgctccgc tcctgttcgc gacgggaccg tttgtcctgc
     4261 tgcggaacaa ctatcgcgcc tatgtacttg aagggcgtcc gcttcgtttc gtgctggccg
     4321 gcgcggcagt cgcgacggtc tgggcgcttc tcacggggtt cctgctgctc ggcctcgcgg
     4381 aatgggcgtc gggctaagta gaaaaggggt ggagccggcc ttccggggaa gaaggccggc
     4441 tccgataagt ccggtcttgg ggacggggag ggtggggacg cgaccggacc taagctttga
     4501 acatcatcag ttccttgttc cggccaccgt cgaggtgtcg tagccgccca gggaaaccca
     4561 acggttcggg ttgtcctgtg cctgacgctt gatgaaccgg tatcctgttt ccgaccaggc
     4621 caggaccttc ggcgcctggt tgtcgagaat gaagtcgcca cggtcggtct tgacggtcag
     4681 cacggcatgc ccgtcaccct tgtggtcccg caccaccgtg atcagcagcg cctggcgggg
     4741 gaagccggcc cggatcaggc gcttgcgctt ctccagcaca taatcttcgc agtcgccctt
     4801 gccctccgtc ggataggacc agtgctcaac aacgccatgg tgctccatgt cggtgattgg
     4861 ggcgatctcg cggttcaccg cggcgttgat gcgcaggatc tcgcgccagt tgccttcgtt
     4921 cagggaaacg atggaggggc gcgtctgcgg cacattgcag tccgacggtt cccgttcgca
     4981 gaactcgacc cagccgatcg gagggctggt ctgctggccg gccggagcga tgtttgaatt
     5041 cagctgctgt gccgttgcga actggccgcc aatgcacgtc agcagcgttg ccgcaaagac
     5101 aatcctcgat atctgagtaa gaccgttgcc caacatttgc cgttccccgt tgttgagcgg
     5161 agaatgccgg ctctcttctt tatttcactt gggattttgc gctagaaacg tgcgtattgg
     5221 attcaggatt tggcagtgaa ttgtcaacac aacagtaggg ttgtcaaagt acgaagatcg
     5281 agccaatcgg attatgatat tgaaatatca cattaaaatg gcgcgcatgc aggggaatca
     5341 gaaaattcta tgtagtgcag tgacgtagta tcaaccagta ttattcagaa aatgaaatgg
     5401 tttatggccg ttcacggtgt tgggcgcccg gcttttggcc gcatttcgac cgcaaagcgg
     5461 cccgccggcg gcctgttcca tgacgcgctg gtgcgcgtgg cggccaggcc aagtgcgcgg
     5521 tcgtgctcaa ggatcaggtg tccctgtcgc ccgggagtct ggtgccgtca gggttctggc
     5581 tactccggcc gaggcagaga gttcggccgg cagcgccttg ccgccgtaca tgtgacggtc
     5641 ggagctcggg caggagcaat gtgcagtgtc cgtcggactg gttcagcccg gtcctgccct
     5701 tcaagccgag gcagtcttgc gcggctgcga cctcacggtc ggcggtgcct cagacctgaa
     5761 aggcctcggg cgctggcgct cgggtgaatt cgaccgcgat gccgcgctcg aacacgcgta
     5821 caaccttggc gggagtctgg ttgagcagaa tcatctcgcc cttgttgaac ggcaaatctg
     5881 tgttgaaagc cgcaccggaa agggaaacgt cgatcagccg gaccatgatt tccgatccgg
     5941 atgccgttac cagccttgca tgtggattgc ggggcacgtg gcgctcatgc cggcggtctt
     6001 cgggcaggcc gagctcgctg cggttcgcaa gccaggtcaa ctgcgctgcc agtttgtcgc
     6061 gccttcgcaa cgtggccgtg atggtcattg caaagccgtc cggcgttctg cgggcgatgt
     6121 tgccctcaat gcgcccgacg gtctcgagat aagccacgac gcgatcgttc acgttgccag
     6181 ccgccgtgca ccgcaggcgg acgccgccgg gggacatgtc gacggtcatg cattcatgtt
     6241 ccgtgcgatc cggaagcatg tatctcccgt gcagcgcgac ttcgacgcgc tggaaacggc
     6301 gcaggtcgac aggacggacg gaaatgcggg agttcggaac gagcacagtg agcgatccag
     6361 gcaatctgtt tgtcaaaccg acgaaaccgg tgcctgagac actattcgtg caccccttaa
     6421 gattggctta agcctaacaa agggttgtgc gttgcgacta gacgtattgc tttgctcaaa
     6481 atcctggcga gccgccgttg aataccacga gatggccgcg ccgctctccc ttgtgcccat
     6541 cgataaccac acccgctgtc ggagggtgca aatgccgggt atcgcgctct ccaatgacgc
     6601 gaaaggacag gatctcaagt ccggctccgg tgcccagggc gaaacggtcc tggcgcttgt
     6661 aggcgagcga tccgaacagc atcacgcctg cgcggccgtt ctggagtagc ggcaacacca
     6721 gcaattccgc gtcgaagctg cgtcgatccg ccatcagcac ggagactcca gcgacaacag
     6781 gaaactggtc ctcggtggcc gtcagaagga tttctccacc gtcatccgcg gcggcctggg
     6841 tcaggaccga caggaattcc cgcccccgga gttcctgtcc aaacaccacg cagaggcttg
     6901 agccggccag ccggaaccga aacgcgccct cagcgtcccg ctccagcagg aagacgtccg
     6961 caagatggcg tcccagtgcg accggatcga tggcgtttcg ctcgggtgcg gtcgcaccgg
     7021 cacgcaggcg acgccagtac gcaaagatgt gcttgctcga ttcaaaccgc atgacgtgcc
     7081 ctggtcctgt ttcttgctgg cacgccgggt cgcacaagtg ccctgtttca gggcagggcg
     7141 ccgcgccatc tggccaacgg agggtgcggt gcagggaaca tgcctaacgg gaccttccag
     7201 aacccgacgg cgggtttaac gttaattcgg gtgagggttt gcggttttgc ccggttcatg
     7261 agacctttac tgcgctccaa acgaattcgc cgccgcgtcg tccagctccc gacgcggcgg
     7321 tttctttttg tgcggccgga tgtccgttca aaatttccgg ttgcaaacgg ccaatcaccg
     7381 ggcaatcctg cgctacgtcc ggccataagg acgggatacg gcggtcgccg catctcacgg
     7441 cgatcccgca gagcggagga acgccatgat tgtttcccac attctcgatc agaaggggcg
     7501 tgacgtagcc acgacgcgtc cgcacctgac catgatggat gtcgtgcggc acttgggaga
     7561 tcggcgcatc ggcgcgctgg tcgtgacggg cgcggatggc gcgatcctgg gtatcgtctc
     7621 ggaacgcgat gtggtgaagg cgatggccaa gggcggggcg gccgcgctcg aagacccggt
     7681 gtcgcgacac atgacggcaa aggtcgtcac ctgcgcaccc gaggatcatg ccgatacggt
     7741 gatggaggcg atgactgccg ggaagttccg ccacatgccg gtagaggccg gcggaaagct
     7801 ggccggcatc atttcgatcg gagacgtcgt gaaggcacgc atcgagcaga tggccagcga
     7861 aagccaggcg ctgcgcgact acatcacgat gtcctgagcc ttcgggctca tgcgcctctg
     7921 agggctgcgt aggcctcgcg gatttccggc agcattctct cggctgcttc cgagccgata
     7981 gcaatcgctt catcggcccg gtgaaattca aacaggccga tcttggccat cttgggagcg
     8041 atcatcacat ccggaggatc gccggccaga cgcgagcggg cgatacgatc ttgcgtgatg
     8101 ttgatggttt ccaccatgac ggcggcgatc gagggcgggc tttcgctatt tgagcgcgga
     8161 ttgaagtatc cggtcacggc atgcgccgcg tttcgcatcg gcttaagaaa tccgccttcg
     8221 gctgcggcag actccggcac cggaggtggt gaagcatgat ggtctgcgat caccgtgcca
     8281 cgcccgaagg tttctgtgtt gacgttcacc gcaataacaa ggtccgcgcc gagcgcgcgc
     8341 gccgtcgtga tcgggattgg attgaccata gcgccatcga gaagccactt gccgccaaca
     8401 cgcaccggct cgaagacacc ggggatggcg taggaggcgc gcatcgcggt caccaggtgg
     8461 ccccgagtca gccagacttc gtgacctgaa ccgaattcgg tggtcaccgc cgcaaagcgg
     8521 gtcggaagat tttcgatggt gcggtccttc aggccgtctt ccagcatgtc gcgcaggcga
     8581 cgcccgccca tcagtccggt tctggacagg ttgaggtcaa gcaggctgaa cacgccgcgc
     8641 ttggtgaggc ggcgcgcgaa aatctccagt tggtcgagac cgccggcact ccagcagccg
     8701 ccgacgaccg cgccgatcga tgttcccgtg atgatcttcg gccgcaggcc ttcgcgttcg
     8761 aggacgcgca ggatgccgat gtgggcccag ccgcgcgccg cgcccgcgcc gagcgccaga
     8821 gcgatgaccg gtggctgcgg acggctgacc ggctgctcct gctcctcagc agggggctgc
     8881 gggtcgcgtc ggaaattgag gacggcaagc aaggcttcct ccagcaactc agtcgggttc
     8941 atcctcccgg acgacccatg gcgcgacccg gtgacaagct tgcgactgaa gtatgactga
     9001 accgctaagc gcctgaactg ccgttgaata tcagagttag cgaaccgtca tcggcacgcc
     9061 tgacgctgcg gtagaaacag ctgcgccggc tggtatggca ggccggaccc gtttgctgca
     9121 ccttcaacag gatcgcgtcc tgatcgcagt caatgctgat ctcgacgacc tgctgcacgt
     9181 ggccgctgct ttcgcccttg cgccagaact tgcgacgcga ccgcgaccag taccaggctt
     9241 cgccggtatc gatcgtgcgc gcgagcgcct ccgcgttcat ccaggccagc atcagcacct
     9301 caccgcttga cgcatcctgg gcgattgcgg ggatcagacc gttcgcatcg aatgtcggtg
     9361 tcagccgttg tccctcttcg gtgtccaact tggttccggc cggggcgaag ggcgttgcgc
     9421 gcgtccacat gtgcgtcctg tcggactgcg cgaggctcaa gtcaaggcct cgcctttccg
     9481 ggccttcgcg cctacaatgc gcctgcgatg ctgaacgacg tgtacaatca acgtattctt
     9541 gagctggcag cggacattcc caggctcggc cgactgccgc agccgcaggc cacggccacc
     9601 gcgcattcga aactgtgcgg ctcaaccgtg accgtcgatc tctgcctgaa cgatggccgc
     9661 gtgagtgcgt tcgcgcatga cgtgaaggct tgcgcactcg ggcaggccgc atcctcgatc
     9721 atggcgagga atatcatcgg atcgacgccg gacgagttgc gcgccatccg ggaaacggtc
     9781 cgccgcatgc tgaaggagaa cggcactgcg ccaagtgatg gacgctggag cgacctgcgg
     9841 gtgctggaac cggtgcgcga ctacaaggcg cgccacgctt caaccctgtt gacgttcgat
     9901 gctgtctgcg aggcgttggg aaagatcgag gcgcaggccg ccgcccagca gcaatagcca
     9961 ggacatcgac tttcagcgtt cgggctgtcc agccccgcgg ggttccggct cggacgaagc
    10021 acgggcgcgg aagccgtcac aggcagcaac ccagggccga tactgctcag gcaaattccc
    10081 aagggcgagc ttcaggctgt agacgctgtc attgccctcg tcgatccgga ggctgaggcc
    10141 atcggacggg cgggtgacgg cattctcgac gacttcggaa accaccgtgc ccgccttggc
    10201 gatccaggcg cccgggttgc gcgccgtcag tcggttaaac gttgccggca tcgcgcggat
    10261 gtcctttccg gatatggcga tgaccgcgtc cttcgtgccg gaaaccagca ggacccgcct
    10321 gagcgccacc ggccgatcct ccgggaggct cttgctgacg ctgagctcga ggcgcgcgtt
    10381 gtggaagccc ctggttgcgt tgcaggtgaa ttctagctgg aaattggctc cggcacgctc
    10441 cgctccgggc aggggcccgc ctgtcaccag cgtggccgtc gatctgccga cgagcagccc
    10501 ccagttgcct tcgcgcgcca gcaacggcgt tgcgtgctcg atagacgcca cgcgaaggtc
    10561 cgtgatttcc ttcggcgtca ggcgcctgat ctgccccttg tgcggtgtcg tcgaggccaa
    10621 ctcgaataga cgcaggtcga catccatttt ctggaggtag acgacgatgc ggctggcggt
    10681 ccgctgggcg ttttcgatgt cacgcgagac gtaattctgc tggtgcagct tctccgcctt
    10741 gtcgctgggc caaaacatat gcacgccgta ggtggcgctg gccgggatgg tgcgcttgac
    10801 cccgccgaga aacgcatagg cgcaggacga attgcattgg ccggagttcg gggagagctg
    10861 gtgccggcgc aagggcgggg catcgcccga cccgaccatt tccgctgtgc cgaccgggag
    10921 ggagcggccg attgaaacct tggcggacag cttgcgcagc cgttcgccca gttcgaggcc
    10981 accgttgatg ctgcctccgg aggagtcgaa atagatcgtt agtccggtga tatcgtgctt
    11041 cttgagaaac tcatcgaaat ccttgggcgt gtcgcggaca atgccgccgc gcgcgctgac
    11101 ccagcgaatc tgcttgtcac ggggcgcttc cggatcgttc gtggccagtt cgaagaccat
    11161 gcggtccggt gccccttccg ccgaagcggg ggctcgccgt tgctgtgcat gggcggcatt
    11221 ggtcgctgcc gcggtcgcgg cggcaatcga caacgcgagc agcatgatcc tggcctgcat
    11281 gcgccaagga tcggcgcgct caggcttcag gtcaagactg gagcgcgctt agagcccctc
    11341 agaagccgga aatgggaaac gaataggcaa gctgtaggcc aacaatgaac tggttcgagg
    11401 aaccgattct tttgacgatc gggctgttgc cgggctcgtt cgcaagcctg ttgtacccac
    11461 catgcagcgt ggcggaccag gaatcgttaa actggtagcc caacgacgaa tagacaccat
    11521 aggacttcac gccaccgccg gcattgtagg tccagaccag cgcattgcgc gaggcatcaa
    11581 gcgccgtcac gccgaaattc gtgcgcatga acttcgaatc cgcgacttcg aggcgcgggc
    11641 cgatggcaaa ggtgaagcgg tcgaaagcct gcacccagtc gaggccgaag ttggcgtgga
    11701 aaccgtcctg gctgcggaaa ccatgccggg cttccgcgcg ggcgcgcaaa ttcggggtga
    11761 tccagaaatt ggcaaacaca ccgggttcga ctgtccattt cacatcatgg atgccggcga
    11821 gccgccaatc gccgccattg taacggccgg agcggatacg ggccaccggg cccatttcaa
    11881 aagggccggt gtcgaccagc gaaaacccga tgccatcatc gggcgaggac cacttgggct
    11941 tggttccggc acggcggaat gacaaagagg gataggcgat gaagccgttg gtgtccgcgc
    12001 catcccactt cgggccgctt accgcattcg ccttgacggt gacgatccat cccgaagccg
    12061 gcgtcggcgt atagggggta ggcttgtcgg cggccatggc ggaaccagcg acagtcatgg
    12121 caaacgcgca ggcgatcgaa cggttggcaa acacggcaga atccccgaca agatttctgc
    12181 tcaaacctag taggtcagtg ttaacccgcc ggcaagagat gtggttaacg gcttgctgac
    12241 cggtcgcctt cgcaagcccg atctgttaag gatttccggg gtctgcggca tgaaaacatc
    12301 acttttgtcc ttgcctgggc gcgccgcatc gctggcgctg atcggcctga tccggctgta
    12361 tcgctacacg ctttctgcct tcatggggcg gacctgccgc taccttccga cctgttcgga
    12421 atacaccgag gaggctatcc gccggcatgg tgcctgggcg ggcggctgga tcggcttcgc
    12481 gcgcatctgc cgctgccggc cgggcggggc c
//
LOCUS       DAOMXN010000049        12493 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_2748_length_12493_cov_11.588841, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000049 DAOMXN010000000
VERSION     DAOMXN010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 12493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12493
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2748_length_12493_cov_11.588841"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..981)
                     /locus_tag="PK812_05695"
     CDS             complement(58..981)
                     /locus_tag="PK812_05695"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020094130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HRJ69085.1"
                     /translation="MKHVAVLMGGMASERPVSLKSGAACARALRDSGFRVTEVDVGTD
                     VAQVLAALKPDVAFNALHGKFGEDGCVQGVLEILKIPYTHSGVLASSLAMRKDQAKVV
                     MKAAGIQVANGVTVNRFEAAKRHALPPPYVAKPVCEGSSFGVVIVKEGRSHPPQELAR
                     TDWPYGDDLLVEAFIPGRELTCAVIGYRDGAKALGVIEILSPSTEFYDYEAKYSKGGS
                     IHVLPAEIKPFVYRQVQEMAARAHEALGCRGVSRADFRYDEPSGTLVCLEVNTQPGMT
                     ETSLVPELAAHAGLTFGELVTWMVEDASLER"
     gene            complement(978..1922)
                     /gene="murB"
                     /locus_tag="PK812_05700"
     CDS             complement(978..1922)
                     /gene="murB"
                     /locus_tag="PK812_05700"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HRJ69086.1"
                     /translation="MTFPDLTASLSAAMPDLRGRLLANQSLAPLTWLRVGGPAQTLFT
                     PADTEDLAYFLQRLPKDIPVTILGLASNMIVRDGGVPGVVIRLAPRGFGQIAIEPGHR
                     LRAGAFASDRKVAMAAAEAGIGGLEFLYGIPGCVGGALRMNAGTRSNTNPEIEGEVRE
                     RFVEATGVTRDGEIRRFNLEDMGFAYRTCSVAPDTVFVEAVMQGFERPVAEIEAINAR
                     VQEHRERDQETKVRTAGSTFKNPPGHSSWRLIRDAGGLDVRIGQAEMSKKHANFLIAH
                     DGATAADIEDLGEEIRRRVKSNSGIDLEWEVKRIGVRP"
     gene            complement(1919..3325)
                     /gene="murC"
                     /locus_tag="PK812_05705"
     CDS             complement(1919..3325)
                     /gene="murC"
                     /locus_tag="PK812_05705"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012319323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HRJ69087.1"
                     /translation="MKIPRSLGPIHFIGIGGIGMSGIAEVLHNLGYTVQGSDAADGAN
                     VKRLRDKGIACFVGHKAENLNEAEVVVVSTAIRKDNPELVTARERRIPVVRRAEMLAE
                     LMRLKSAVAIAGTHGKTTTTSVVATLLEAGGFDPTVINGGIINAYGTNARLGGGDWMV
                     VEADESDGTFLKLPADIAIVTNIDPEHLDHFKTFDAIKDAFRSFVNNLPFYGFAVMCL
                     DHPTVQQMVGEIQDRRILTYGENPQADFRILDIDLSGGQSRFTLLVRDRKRGTDTVIR
                     DLLMPMPGHHNALNATAAIAVAYDLGVPVETIRRALAGFGGVKRRFTKTGEWQGALIF
                     DDYGHHPVEIAAVLRAARHSTKGQVIAVVQPHRYTRLQSLFAEFAACFNDADTVIVAE
                     TYSAGETPIAGADRDSLVSAIKARGHRHALALPGPEALAGMLADVAKPGDYIVCLGAG
                     NITQWAYALPAELEALKR"
     gene            complement(3367..4458)
                     /gene="murG"
                     /locus_tag="PK812_05710"
     CDS             complement(3367..4458)
                     /gene="murG"
                     /locus_tag="PK812_05710"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HRJ69088.1"
                     /translation="MTPRQGSVLLAAGGTGGHLFPAEALANALIARGVTVDLVSDERA
                     ARYADRFPAKLHQITSGTVTGGGWLGKLKGALLLLKGTQESLGLLSRLKPAAVVGFGG
                     YPTVPPVYAATMRGIPTVIHEANAVMGRANRFVAPRVTRIATGFPLRSDAFAAKTVVT
                     GNPVRPPVLEAAATPMPTLQAGGALNLLVFGGSQGARIMSDIVPPALEKLTAGERARI
                     RLVQQVREEDLARVSETYARIGIDAEIAPFFKDLPSRIAAAQLVVSRSGASTVAELAV
                     IGRPSILVPLPGSLDQDQAANARVLADCGGATLQLQAGFTPDWLADALRKALAEPERL
                     SAMATAARSAGTPDAADRLADLVIRTAGL"
     gene            complement(4455..5609)
                     /gene="ftsW"
                     /locus_tag="PK812_05715"
     CDS             complement(4455..5609)
                     /gene="ftsW"
                     /locus_tag="PK812_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HRJ69089.1"
                     /translation="MVSRAERTEFNEWWWTVDRWLLFSFLALMVLGILFGLAASPPVA
                     SRLGLSTFHFVNRQAAFLAVALVVMIAVSAMPLRQIRRMALLLFCVALALVCATLVFG
                     AEVKGARRWLSLGGLSVQPSEMLKPAFVVLTAWAFSEKAVRDDMPSTTLALLLLPLVV
                     APLILQPDLGQTMLVSAVWCGLFFLAGLHILWVIGLGGVGVMGLLLAYKFLPHVTARF
                     DRFLNKGDGAGAFQAETARESLLSGGWYGKGPGEGTVKRILPDSHTDYIFAVIGEEFG
                     ILICLVVVAIFGLIVVRSLILAYRSEDPFVRFATAGLAMLFGLQASINIAVNLDLIPP
                     KGMTLPFISYGGSSLVSLAITAGCLLALTRRRPSAAIVHTLPQNLGAATA"
     gene            complement(5611..7005)
                     /gene="murD"
                     /locus_tag="PK812_05720"
     CDS             complement(5611..7005)
                     /gene="murD"
                     /locus_tag="PK812_05720"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HRJ69090.1"
                     /translation="MTPVTTFKDRKVALFGLGGSGLVAAQALMAGGSIVSAWDDSEAA
                     RLAASARGVAIVDLKAADWSDFSALVLAPGVPLTHPEPHWTVPLAQAAGVEILGDIEL
                     FCRERAARAPNAPLVCITGTNGKSTTTALIAHLLRAAGRDVQLGGNIGTAVLALEPPA
                     EARHHVIEVSSFQIDLAPSLAPSIGVMLNLTPDHIERHGTVEHYAAVKERMVSAATLA
                     IVGVDDEMSAAMASRRDQAGKATVRISVRSPLPNGLFADGSKIVFARKRKRIEAADLT
                     GIGSLRGRHNAQNAAAAIAVATTLGIDGQVLREGLKSFAGLPHRMEEIGRVGGSIFIN
                     DSKATNADSTEKALAAFEKDLFWIIGGKAKDGGITSLASYFPRIRKAYLIGTAAAEFA
                     RTLDGAVPYAHAGTLDVAVQMALEDAQRHRGGVILLSPACASYDQYKNFEQRGDHFRA
                     LVEALPGFVSRKGA"
     gene            complement(7013..8098)
                     /gene="mraY"
                     /locus_tag="PK812_05725"
     CDS             complement(7013..8098)
                     /gene="mraY"
                     /locus_tag="PK812_05725"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HRJ69091.1"
                     /translation="MLFWLSDFSAQFQPFNLFRYITFRAGGATVTALFFVLMFGPKII
                     SLLRVKQGKGQPIREDGPESHLLTKKGTPTMGGLMILSGVVVASLLWANLSNPFVWTV
                     LCVTLGFGAIGFYDDYLKVTKQSHKGFSGRSRLGLETAIAGVAVYIMMQYMPPALAGA
                     ISFPVFKDAMVNVGVFFIVFGVFIIVAAGNAVNLTDGLDGLAIVPVMIAAGTFGFIAY
                     LAGNAIFANYLQINFIPRTGELAVICGALIGAGLGFLWFNAPPAQIFMGDTGSLALGG
                     LLGSVAVATKHEIVFAIVGGLFVLEAMSVIIQVASFKLTGKRVFRMAPIHHHFEKKGW
                     QEAQVVIRFWIISVILALIGLATLKLR"
     gene            complement(8110..9537)
                     /locus_tag="PK812_05730"
     CDS             complement(8110..9537)
                     /locus_tag="PK812_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamyl-2,
                     6-diaminopimelate--D-alanyl-D-alanine ligase"
                     /protein_id="HRJ69092.1"
                     /translation="MTVLWSGTALVDALGADAPAGMADIAGLSIDTRTLLRGDLFVAL
                     KDVRDGHDFVANAFKAGAAAALVDRAHASALAGLGALYVVDAVLPALERLGRAGRARM
                     RGHVIAVTGSVGKTSTKEMLRTVFQRQGSTHASVASYNNHWGVPLTLARMPEDTAFGI
                     FEIGMNHSFEILPLTAQVSPQVAIVTTVEPVHLASFPSLAAIADAKGEIFSGLEPGGT
                     AVLNRDNPQFDRLKAHVAASPAGRIISFGEHADAHVRLLSANCAADSSTVEADVLGEL
                     VAYQIGAPGRHLVQNSLAVLAAVHATGADLALAALALRAFAPADGRGRKIQLGTGTGP
                     ITLLDESYNANPSSMRAAFAVAGTTPVGPRGRRVAVLGDMLELGDQGAELHRDLAQAL
                     EKSGIDLVFAAGPLMKNLYDALPKTRRGGYAADSAALEAAVLDGVRGGDVVTVKGSNG
                     SRMGRIVTALKTRYPVISPADDRDV"
     gene            complement(9534..11000)
                     /locus_tag="PK812_05735"
     CDS             complement(9534..11000)
                     /locus_tag="PK812_05735"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HRJ69093.1"
                     /translation="MSGDFAELSHRLGLQPEPSRPAVTGIAFDSRKVQPGNVFFALAG
                     SRTDGAKFIADAARAGAAAIIGEAARPADLPATVIYARVDDARLALARAAAWFHPRQP
                     GTILAVTGTAGKSSVADFTRQIFQALGHESASLGTLGVISRQRASYGSLTTPDPVALH
                     QELDGLVEEGVTHLAIEASSHGLDQRRLDGLRLTGGAFTNLGRDHLDYHATVDDYLKA
                     KLRLFTALLPTGAPVVINRDGDRSGDVLATCEKQGLKPFTTGRKGEHLRLLSVGEVPF
                     GQALELAFGRHVHAARLPLIGGFQVENALVAAGFALALGEAPKAVFEALARLTGVPGR
                     LERVGARGTAPVFVDYAHKPEALAHVLDAVRPSVRGRLIVVFGCGGDRDKGKRPIMGA
                     IAADKADIVIITDDNPRSEEPVQIRREILMAAPGALEIGDRAAAIREAVAMLKDGDAL
                     IVAGKGHETGQIVGSVTRPFSDHDEARAALHSVGGTPS"
     gene            complement(11003..>12493)
                     /locus_tag="PK812_05740"
     CDS             complement(11003..>12493)
                     /locus_tag="PK812_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRJ69094.1"
                     /translation="ADVKMVSVFAEPRRIVDKDEAVELLNAVFPDLDGSDLRAKLGSR
                     KGFVWVKREITPRQQAEVHRLGIPGVGFLPEHKRVYPNGPVAAHVLGFANVDNQGIAG
                     IEKYIDGKGMADLKGTGFAGKPMDLKPVQLAIDLRVTHAMRQELQEAMKKFKAKAAGG
                     LVVDVNTGEIVAQVSLPDYDPNVPTDALKPENINHLAVGVYEMGSTFKALTTAMALDS
                     GKVSLTSNFDATQPLRYGRFKINDYHGQRRVLSVPEIFVYSSNIGTARMALSVGVQGH
                     KDFLKKMGQLDRLITEMPETAKPLVPSNWGELNTMTISFGHGLAVSPLQAVAATAALV
                     NGGYLVPPTYLKRSEAEAKSIAKRVIKPETSEAMRFVMRLNAERGTAGKANIPGYYVG
                     GKTGTSEKVVGGRYSKTKVMTTFMAIAPADKPRYLFLTLLDEPQGSSETHGYATAGWN
                     AAPTTGKIIERVGPMLDIHPRFEIPVQPFPQVSRLNLRMMTQTEPR"
BASE COUNT         2213 a         4202 c         3997 g         2081 t
ORIGIN      
        1 gctcggccca ggcggtgcgc caggcgtcgg cgagcgaagg cctgcgggcc gccgccttca
       61 acgctccagg gaagcgtcct ccaccatcca tgtcaccagc tcaccgaacg taaggcccgc
      121 atgggccgcg agttcgggca caagcgacgt ctcggtcatg ccgggttgcg tgttaacttc
      181 aagacagacg agcgttccgg aaggctcgtc ataccggaag tccgcgcggc ttacgccacg
      241 gcagccgagc gcctcatggg ccctcgccgc catttcttgg acctgtcggt aaacaaatgg
      301 tttaatttcc gctggcagga cgtgaattga tccgcctttt gaatattttg cctcgtagtc
      361 gtaaaactca gtcgacggtg acaggatctc gatcacgccc agcgccttcg ctccatcccg
      421 gtatccgatc acggcgcagg tcagttcgcg gcccggaatg aaggcctcga ccagcaggtc
      481 gtcgccgtag ggccagtccg tgcgcgccag ttcctgcggc ggatgcgaac ggccttcctt
      541 gacgatgacc acgccgaagg acgagccctc gcacaccggc ttcgccacat agggcggtgg
      601 caacgcgtgg cgcttggcgg cttcgaagcg gttcacggtg acgccgttgg cgacctgtat
      661 cccggcggcc ttcatcacca ccttcgcctg atccttgcgc atggccagcg aggaggccag
      721 cacgccggaa tgcgtgtagg ggatcttgag gatttcgagc acgccctgca cgcagccgtc
      781 ctcgccgaac ttgccgtgca gcgcgttgaa cgccacatcc ggcttcagcg ccgcgagcac
      841 ctgggcaaca tcggttccga cgtcgacttc cgtgacacga aacccggaat cgcgcagcgc
      901 cctggcgcac gcagcgccgg acttgagact caccggccgc tcgctggcca tgccgcccat
      961 cagaaccgcg acgtgtttca tggcctcact ccgatcctct tgacttccca ttccaggtcg
     1021 atgccgctgt tggacttcac gcggcggcgg atttcctcac cgagatcctc gatgtcggcg
     1081 gcggtcgccc cgtcatgcgc gatcaggaag ttcgcgtgct tcttcgacat ctccgcctgc
     1141 ccgattcgca catcgaggcc gccggcgtcg cggatcagcc gccaggagga atggcccggc
     1201 gggttcttga acgtcgagcc tgcggtccga accttggttt cctgatcgcg ctcgcgatgt
     1261 tcctgcacgc gcgcattgat cgcctcgatc tcggcgacag ggcgctcgaa accctgcatc
     1321 accgcctcga cgaacacagt atccggcgcg accgagcagg tgcgataggc aaagcccatg
     1381 tcctctaggt tgaagcgccg gatctcaccg tcgcgcgtca cacccgtcgc ctcgacgaac
     1441 cgctcgcgca cctcgccctc gatctcggga ttggtgttcg agcgggttcc ggcgttcatc
     1501 cgcagcgccc cgccgacgca gcccggaatg ccgtagagaa attccagccc gccgataccg
     1561 gcctccgctg ctgccatcgc caccttgcga tccgaggcga aggcgcccgc gcgcagccga
     1621 tggcccggtt ctatcgcgat ctgcccgaag cctcttggcg caaggcggat gaccacgccc
     1681 ggcacgccgc cgtcgcggac gatcatgttg gatgccagcc caaggatcgt gaccgggatg
     1741 tccttcggca gccgttgcag gaaataggcg aggtcttcag tatctgctgg cgtgaacagc
     1801 gtctgcgccg gcccgccgac gcgcagccag gtcagcggcg ccagcgactg gttggcgagc
     1861 agacggccgc gcagatccgg catggcggcg gaaagcgaag cggtgagatc tgggaaggtc
     1921 accgcttcag cgcctccaat tcggccggca gcgcataggc ccactgggtg atgttgccgg
     1981 cgccgaggca aacgatgtaa tcgcccggct tggccacatc cgcgagcatt ccggccagcg
     2041 cttccggccc aggcagcgcc agcgcatgac gatgtccgcg cgccttgatt gccgaaacca
     2101 gactgtcgcg atccgcgccc gcgataggcg tctcgccggc ggagtaggtc tcggcgacaa
     2161 tgacggtgtc ggcatcgttg aaacaggccg cgaattcggc gaacagcgac tgcaggcggg
     2221 tatagcggtg cggctgcacg acggcgatca cctgcccctt ggtggagtgg cgcgcggcgc
     2281 gcagaaccgc ggcgatctcc accggatggt ggccgtaatc gtcgaagatc agcgcgccct
     2341 gccattcccc ggtcttggtg aagcgccgct tcacgccgcc gaacccggca agcgccctgc
     2401 gaatcgtttc caccggcacg ccgaggtcgt aggccaccgc gatcgccgcc gtggcattga
     2461 gcgcgttgtg atggcccggc atcggcatga gcagatcgcg gatcactgtg tcggtgccgc
     2521 gcttgcggtc gcgcaccagc agggtgaagc ggctttgccc gccactcaga tcgatgtcga
     2581 ggatgcggaa atccgcctgc gggttctcac cataggtgag gatgcgcctg tcctgaatct
     2641 cccccaccat ctgctgcacc gtcggatggt cgagacacat aacagcaaag ccgtagaacg
     2701 gcaggttgtt cacgaaggag cggaaggcgt ccttgatcgc atcgaaggtc ttgaaatgat
     2761 cgaggtgttc cggatcgata ttggtgacga ttgcgatgtc ggcggggagc ttaaggaagg
     2821 tgccatcgct ttcgtcggcc tcgaccacca tccagtcgcc gccaccgagg cgcgcgttgg
     2881 tgccgtaggc attgatgatg ccgccgttga tcacggtcgg gtcgaaaccg ccggcctcca
     2941 gcagcgtcgc gaccaccgag gtcgtcgtcg tcttgccgtg cgtgccggcg atggcgacgg
     3001 ccgacttgag ccgcatcagt tcggccagca tttcggcgcg acgcaccacg gggatgcgcc
     3061 tttctcgcgc cgtcaccagt tccggattgt ccttgcggat cgcggtcgag accacgacca
     3121 cttccgcctc attgaggttc tcggccttgt gtccgacaaa acaggcgatg cccttgtcgc
     3181 gcaggcgctt gacgttggcc ccgtccgccg cgtccgatcc ctgcaccgtg tagccgagat
     3241 tgtgcagcac ttccgcgatg ccggacatgc cgatgccgcc gatgccaatg aagtggatgg
     3301 gcccaagcga acggggaatt ttcatgcgaa tcgttccgga aacggccagg agccaggaaa
     3361 tttggcctat agcccggccg tccgaatgac aagatcggcc agccgatcgg ccgcgtccgg
     3421 tgttcctgcc gaacgggcgg ctgtggccat cgccgagagc cgttcgggct ccgccaacgc
     3481 cttgcgcaag gcgtccgcca gccagtccgg cgtgaacccg gcctgcaatt gcagcgtcgc
     3541 gccgccgcaa tctgccagca ccctggcgtt cgcggcttga tcttgatcca gactgcccgg
     3601 caacgggacg aggattgacg gccggccgat caccgcgagt tcggcaaccg tcgaggcccc
     3661 ggaccggctg acgacaagtt gcgcggcggc aatgcgcgac ggcaggtcct tgaagaacgg
     3721 cgcgatttca gcgtcgatgc cgatgcgggc gtaggtttcg ctcacccgcg caaggtcctc
     3781 ctcgcgcacc tgctggacca gtcggatgcg tgcccgctct cccgctgtaa gtttttccag
     3841 cgccggtgga acgatgtcgc tcatgatccg cgcgccctgg ctgccgccga aaaccagaag
     3901 gttcagcgcg ccgccggcct ggagggtcgg catcggcgtc gcggcagctt cgagaacggg
     3961 cgggcgcacg ggattgccgg tcaccaccgt cttcgcggcg aacgcatcgc tcctgagcgg
     4021 aaatcccgtg gcgattcgcg tgacgcgcgg agcgacgaag cgattggccc gccccatcac
     4081 ggcgttggcc tcgtggatca cggtcggaat gccgcgcatc gttgcggcat agaccggcgg
     4141 gacggtcgga tatccgccaa aaccgacgac cgccgccggc ttcaggcggg agagcaaccc
     4201 caggctttcc tgcgtgccct tgagcaggag cagcgcaccc ttcagtttgc cgagccatcc
     4261 gccacccgtc accgtgccgc tggtgatctg gtgcagcttc gccggaaacc ggtcggcgta
     4321 gcgggccgcg cgctcgtcgc tgaccaggtc gaccgtcacg ccacgggcga tcagcgcatt
     4381 ggccagcgct tcggccggaa acaggtgacc accggtgcca ccggcggcaa gaaggacact
     4441 gccctgccga ggagtcatgc cgtcgccgct cccaggttct gcgggagtgt gtgcacgatg
     4501 gcggcggacg ggcggcggcg cgtgagcgcc agcaggcacc ctgccgtgat cgccagcgac
     4561 acgagcgagg agccgccata cgagatgaac ggcagggtca tgcccttcgg cggaatgaga
     4621 tccaggttca ccgcgatgtt gatgctggcc tgaaggccga acaacatggc cagacccgcc
     4681 gtcgcaaagc ggacgaaggg atcttcgctg cgataggcca ggatcaacga ccgcaccacg
     4741 atgagcccga agatcgccac cacgacgaga cagatcagga tgccgaactc ctcgccaata
     4801 acggcgaaaa tgtagtcggt gtggctgtcc ggcaggatgc gtttcaccgt gccttcgccc
     4861 ggccccttgc cgtaccagcc cccggacagc aggctttcgc gcgccgtctc cgcctgaaac
     4921 gcgccggcgc cgtcgccctt gttgaggaac ctgtcgaagc gtgcagtcac atgcggcagg
     4981 aacttgtagg caagcagcaa gcccatcacg ccaacgccac cgagaccgat cacccagagg
     5041 atgtgaaggc cggcaaggaa gaacaatccg caccacacgg ccgacaccag catcgtctgg
     5101 ccaagatccg gctgcaggat cagcggcgcg acgacgagcg gcagcagaag caaggccagg
     5161 gttgtgctcg gcatgtcatc acgaaccgcc ttctcggaga aggcccaggc ggtcaggacc
     5221 acgaaagcgg gcttcagcat ctctgagggc tgtacactca gcccgcccag cgacagccag
     5281 cggcgcgcgc ccttcacctc ggctccgaag acaagcgtcg cgcaaaccag cgccagggca
     5341 acgcagaaca gcagcagcgc catgcgccgg atctgcctga gcggcatggc ggagacggcg
     5401 atcatcacga ccagcgcgac cgccaggaag gcagcctgac ggttgacgaa gtggaacgtt
     5461 gaaagcccaa gccgcgacgc cacgggtggg ctcgcggcga ggccgaatag gatgccaagc
     5521 accatcagcg ccaggaagct gaacagcagc cagcgatcga cggtccacca ccattcgttg
     5581 aattcggtgc gctcggcccg tgaaaccatg tcaggctccc ttgcggctga cgaagcccgg
     5641 caaggcttcg acgagcgcgc ggaagtggtc gccgcgctgc tcgaagttct tgtactgatc
     5701 gtaggaggca caggccggcg aaagcaggat cacgccgccc cggtggcgct gcgcatcctc
     5761 cagcgccatc tgcaccgcaa catcgagcgt gccggcgtgg gcatagggca cggcgccatc
     5821 cagcgttctc gcgaactcgg cggcggcagt gccgatgagg taggccttgc ggatgcgcgg
     5881 gaaatatgag gcgagcgagg tgatgccgcc gtccttcgcc ttgcctccga tgatccagaa
     5941 caggtccttt tcgaacgctg ccagggcctt ttcggtggaa tcggcgttgg tagccttgga
     6001 gtcgttaatg aagatcgagc cgcccacccg gccgatctcc tccattcggt gaggcagccc
     6061 ggcgaagctc ttcaacccct cgcgcagaac ctggccgtcg atccccagag ttgtcgccac
     6121 agcgatcgcg gccgcggcat tctgggcatt gtgccggccg cgcaacgatc cgatgccggt
     6181 aaggtcggcc gcctcgatcc gcttgcgctt gcgcgcgaag acgatcttcg atccgtccgc
     6241 gaacagcccg ttcggcaatg gcgaacgaac cgatatgcgc accgttgcct tgccggcctg
     6301 atcgcggcgc gatgccatgg ccgccgacat ctcgtcgtcg acacccacga tggccagggt
     6361 cgcggccgac accatccgtt ccttgacggc ggcataatgt tcgaccgtgc cgtggcgttc
     6421 gatatggtct ggcgtaagat tcagcatcac gccgatgctc ggggccagcg agggcgccag
     6481 gtcgatctgg aacgacgaga cctcgatcac atgatggcgc gcttcggccg gcggttcgag
     6541 cgcaagaacg gcggtgccaa tattgccccc gagctgcacg tcccgccccg ccgcgcgcag
     6601 gaggtgggcg atcagcgccg tcgtcgtcga cttgccgttc gtaccggtga tgcagacaag
     6661 cggtgcgttg ggagcccgcg cggcccgctc gcggcagaac agctcgatat cgccgagaat
     6721 ttccacgccg gcggcctgcg ccaacggcac ggtccagtgc ggctccgggt gcgtcaatgg
     6781 aacgcccgga gccaggacaa gcgccgaaaa gtccgaccag tcggcagcct tcagatcgac
     6841 gatggcgacg cccctggcgc ttgccgccag gcgtgcggcc tcgctgtcgt cccaggccga
     6901 gacgatggag ccgcccgcca tgagggcctg cgcagcgacc agccccgagc cgcccaggcc
     6961 gaacagggcg accttcctgt ccttgaaggt ggtgaccggg gtcatgggcg catcaccgca
     7021 gcttcagggt agcgaggccg atgagggcca ggataaccga gatgatccag aaccggatca
     7081 caacttgcgc ttcctgccag cccttcttct cgaaatggtg gtggatcggc gccatgcgga
     7141 acacgcgctt gccggtcagc ttgaacgaag cgacctggat gatcacgctc atcgcttcca
     7201 gcacgaaaag cccgccgacg atggcgaaaa caatctcatg cttggtggcg accgccacgc
     7261 tgcccagcag tccgccaagc gcgagcgagc cggtatcgcc catgaagatc tgcgccggcg
     7321 gcgcattgaa ccacaggaag ccaaggcccg cgccaatcag cgcgccgcat atcaccgcga
     7381 gttcgccagt ccgtggtatg aagttgatct gaaggtagtt cgcgaagatc gcattgccgg
     7441 ccagataggc gatgaacccg aaggtccccg ccgcgatcat caccggcacg atggcaaggc
     7501 catcgaggcc gtccgtgaga ttcacggcat tgcctgcggc aacaatgatg aacacgccga
     7561 agacgatgaa aaataccccg acattgacca tcgcatcctt gaacaccggg aaggagatcg
     7621 caccggccag cgctgggggc atgtactgca tcatgatata gaccgcgaca ccggcaatcg
     7681 ccgtctcgag gccgagccgg gagcggccgg aaaagccctt gtggctctgc ttggtcacct
     7741 tcaggtagtc gtcgtagaaa ccgatcgcgc cgaaccccag cgtgacgcac aggaccgtcc
     7801 agacaaacgg gtttgagagg ttggcccaca ataggcttgc cacgaccaca ccggacagga
     7861 tcatcagtcc gcccatggtt ggcgtgccct tcttggtcag cagatgggat tctggcccat
     7921 cctcgcggat cggctgacct ttgccctgct tgacacgcag caggctgatg atcttcggcc
     7981 cgaacatcag cacgaagaac agggccgtca ccgtcgcacc gcccgcccgg aaggtgatgt
     8041 agcggaacag gttgaacggc tggaactgcg cactgaaatc ggaaagccag aacagcattc
     8101 gcttttgcct cacacgtctc ggtcgtccgc aggggaaatg accggatatc gcgtcttgag
     8161 cgctgtcacg atgcggccca tgcggctccc gttcgagccc ttgactgtaa cgacgtcgcc
     8221 cccgcgaacg ccgtcgagca cggccgcttc gagcgcggcc gagtcggctg cgtatccgcc
     8281 ccgacgggtc ttcggcaagg cgtcatagag gtttttcatc agcgggccag ccgcgaagac
     8341 caggtctatc cctgattttt caagagcttg cgccaagtcg cgatgcagct ctgcgccctg
     8401 gtcgccaagc tccagcatgt cgcccaggac ggcaacgcgg cgtccgcgag gtccaacggg
     8461 cgttgtaccc gccaccgcga aagccgcccg catggacgac ggattggcgt tgtagctttc
     8521 atcgagcagc gtgatcggac cggttcccgt cccaagctgg atcttgcgtc cacgcccgtc
     8581 ggcaggggcg aaggcgcgca gggccagcgc cgcgagcgcc agatcggcgc cggtcgcgtg
     8641 caccgcggcg agaacggcga ggctgttctg gaccagatgc cggcccggcg ccccgatctg
     8701 gtaggcaacc agttccccga gaacatcggc ttccaccgtc gaggaatcgg cggcgcaatt
     8761 ggccgacagc aggcgcacat gcgcgtccgc gtgctcgccg aagctgatga tgcgtcccgc
     8821 cggcgaggcc gcgacatgag ccttcagccg gtcgaattgc ggattgtcgc ggttgagaac
     8881 cgccgtgccg cccggctcca gtccggagaa gatttccccc ttggcgtcgg cgatggcggc
     8941 cagactggga aagctcgcca gatgcaccgg ctcgaccgtc gtaacgatgg cgacctgcgg
     9001 cgaaacctgc gcggtcaggg gcaggatctc gaaagaatgg ttcatgccga tttcgaagat
     9061 gccgaaggcg gtatcctccg gcatgcgcgc cagtgtgagc ggcacgcccc agtggttgtt
     9121 gtaggacgcg accgaagcat gcgtgctgcc ctgacgctgg aagacggtgc gcagcatttc
     9181 cttggtcgag gtcttgccca ccgagccggt gacggcgatg acgtgccccc gcatcctggc
     9241 cctgccggca cggccgaggc gttccagcgc gggaagcacc gcgtccacca catagagcgc
     9301 tccaagcccc gcgagggcgg acgcatgcgc ccggtcgacc agcgccgcag ccgcgccggc
     9361 cttgaaggcg ttcgccacga agtcatgccc gtcacgcacg tccttgagcg ccacgaacag
     9421 gtcaccgcgc aacagcgtcc gcgtgtcgat tgaaagcccg gcgatgtccg ccataccggc
     9481 aggcgcgtcc gcgcccagcg catccacaag ggcggttccg ctccacagca cggtcacgac
     9541 ggggttcctc caacggaatg aagcgcggcg cgcgcttcgt catgatccga gaacggtctg
     9601 gtcacgctgc cgacgatctg gcccgtctcg tgccccttgc ccgcgacgat caacgcatcg
     9661 ccatccttca gcatggcgac ggcctccctg atggcggcgg cgcggtcgcc gatctcgagc
     9721 gcgcccggcg ccgccatcag aatctcgcga cgaatctgca cgggctcttc gctgcgcgga
     9781 ttgtcgtcgg tgatgatcac aatgtcagcc ttgtccgccg cgattgcgcc catgatcggc
     9841 cgcttgccct tgtcccggtc gcccccgcag ccgaagacca cgatcaatcg ccccctgaca
     9901 ctcgggcgca ccgcatccag cacatgggcg agcgcttcgg gcttgtgggc atagtcgacg
     9961 aacaccggcg ccgtgccccg cgcgccgacg cgctccagcc tgcccggcac gcccgttaac
    10021 cgcgcgagag cctcgaacac cgctttcggc gcctcgccca gcgccagggc gaaaccggcc
    10081 gcgaccagtg cgttctcgac ctgaaagccg ccgatcagcg gcagacgggc ggcatgcaca
    10141 tgccttccga atgccagttc cagagcttgg ccaaagggca cctcgcccac tgacagcagg
    10201 cgcaagtgct cccctttccg gccggtggtg aatggtttca gcccctgttt ttcgcaggtc
    10261 gcgagtacat cgccggaacg gtcgccgtcc ctgttgatca ccactggcgc gccggtcgga
    10321 agcagggctg tgaacagccg caacttggcc ttcaggtagt cgtccaccgt cgcatggtag
    10381 tcgagatggt cgcggccgag attggtgaag gcgccgccag tgagccgcag cccatcaagc
    10441 cgcctctggt cgaggccgtg cgatgatgcc tcgatggcca gatgcgtcac gccctcctcc
    10501 acaagtccgt ccagctcctg gtgcagggca accggatcgg gcgtcgtcag cgagccgtac
    10561 gacgcgcgct gccgcgagat caccccgagc gtgccaaggc tcgcggattc gtgcccaagc
    10621 gcttggaaga tctggcgggt gaaatccgca accgaggact tgcccgccgt tccggtcacc
    10681 gccaggattg ttccgggctg gcgcgggtga aaccatgccg ctgccctcgc gagagcaagc
    10741 cgcgcgtcgt cgacacgcgc atagatcacg gtcgccggca gatcggcagg cctcgccgcc
    10801 tcgccgatga ttgccgccgc tccggccctg gccgcgtcgg cgatgaactt cgcgccatcc
    10861 gtccgcgatc cggccagcgc gaaaaacacg ttgcccggct gcaccttgcg gctgtcgaag
    10921 gcgatgccgg tcacggctgg acgcgacggc tccggctgca acccgaggcg atgcgacagt
    10981 tcggcgaagt caccggacat ggtcagcgtg gctccgtctg ggtcatcatg cgcaggttca
    11041 ggcgcgacac ctgcgggaac ggctgcaccg ggatctcgaa ccgcggatgg atgtccagca
    11101 tcgggccgac gcgctcaatg atctttccgg tcgttggcgc cgcattccag ccggcggtcg
    11161 cgtagccatg cgtttcgctg gagccctgcg gctcgtcgag cagggtcagg aacagatagc
    11221 gcggcttgtc ggcaggggcg atggccatga aggtcgtcat caccttggtc ttggaatagc
    11281 ggcctccgac caccttctcg gatgtacccg tcttgccccc gacatagtag cctggaatgt
    11341 tcgccttgcc ggccgtgccg cgttcggcgt tcagacgcat gacgaaccgc attgcttcgg
    11401 aggtttccgg cttgatgacc cgctttgcga tagactttgc ctcggcttcc gaccgtttca
    11461 gataggtcgg tgggacaagg tagccgccgt tcaccagagc cgccgtggcc gcgaccgcct
    11521 ggagcggcga caccgcgagg ccgtggccga aggaaatggt catggtgttg agttcgcccc
    11581 agttcgaggg aacgagcggc ttcgccgttt ccggcatctc ggtgatcagc cggtcgagct
    11641 ggcccatctt cttcaggaag tccttgtgcc cctgcacccc gaccgataga gccatgcgcg
    11701 cggtgccgat gttcgacgag tagacgaaga tctccggcac gctcagcacg cgccgctggc
    11761 cgtggtagtc gttgatcttg aatcggccgt agcgcagcgg ctgcgtggcg tcgaagttcg
    11821 acgtcagcga gactttgccg ctgtcgagcg ccattgccgt ggtcagcgcc ttgaacgtcg
    11881 accccatttc atagacgccg accgccaggt gattgatgtt ttccggcttc aaggcatcgg
    11941 taggcacgtt cggatcgtag tccggcagcg agacctgcgc gacgatttcc ccggtgttga
    12001 catcaaccac cagcccgccc gccgccttgg ccttgaactt cttcatcgct tcctggagtt
    12061 cctgccgcat cgcgtgcgtg acgcgcaggt cgatggcaag ctgcaccggc ttcaggtcca
    12121 tgggcttgcc ggcgaaaccg gtgcccttca gatcggccat gccctttccg tcgatgtact
    12181 tctcgatccc ggcaatgccc tggttgtcga cgttggcgaa acccagaaca tgcgccgcga
    12241 ccggcccgtt cggatagacg cgcttgtgct cgggcaggaa cccgaccccg gggatgccga
    12301 gccggtgcac ttccgcctgc tggcgcggcg tgatctcacg cttcacccag acaaacccct
    12361 tgcggctgcc cagcttggca cgcaggtcgc tgccatcaag gtcggggaac accgcgttca
    12421 ggagttcgac cgcctcgtcc ttgtcgacga tgcgccgcgg ttccgcgaag accgagacca
    12481 tcttgacgtc ggc
//
LOCUS       DAOMXN010000050        12341 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_2782_length_12341_cov_11.434560, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000050 DAOMXN010000000
VERSION     DAOMXN010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 12341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12341
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2782_length_12341_cov_11.434560"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..2051)
                     /locus_tag="PK812_05745"
     CDS             complement(150..2051)
                     /locus_tag="PK812_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA synthetase"
                     /protein_id="HRJ69095.1"
                     /translation="MGKGAYAALYAGWKNDPQAFWGEAAKSLDWFTVPKVVFDAKAGV
                     YGRWFPDGVCNTAHNCLDRHVAGGRGAQAALVYDSPVTNSKASLSYAELTDEVKVLAE
                     IIRGFGVKKGDRVLLYMPMIPEAVIGMLACARVGAVHSVVFGGFAARELATRIDDCAP
                     KLILSASCGIEPGRIVKYKPLLDEAISLSANKPAACLIFQRPMETAALQAGRDHDWAA
                     ARDAMRASGKAAGSACEPMQATDPLYVLYTSGTTGKPKGVVRDNGGHMVALHWSMKAL
                     YGVDAGDAFWAASDVGWVVGHSYICYAPLLKGCTTIVYEGKPVGTPDPGAFWRVISEN
                     KVKAFFTAPTAFRAIKKEDAEAKFLKTYDISCLQALYLAGERADPDTVKWAEDILGVP
                     VIDHWWQTETGWCIAGNPRGIELLPVKRGSPTVPMPGYDVQILDEGGKPVPDGKMGSI
                     AIKLPLPPGALPTLWQADDRFEQSYLTEFKGYYSTSDAGYRDEDGYLYIMGRTDDIIN
                     VAGHRLSTGGMEEVLSSHPSVAECAVVGIKDSLKGEAPLGFVVLKAGVTKTAAEVEKE
                     CAALVREKIGPVAAYKQTVVVNRLPKTRSGKILRGTMKKIADGDAWSMPATIDDPATL
                     EEIGKALGK"
     gene            2256..2531
                     /locus_tag="PK812_05750"
     CDS             2256..2531
                     /locus_tag="PK812_05750"
                     /inference="COORDINATES: protein motif:HMM:NF025122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4164 family protein"
                     /protein_id="HRJ69096.1"
                     /translation="MSRETILEALARCGAALDRLEAASLRHQEGERLRMNLESELALM
                     RADRQALAEKLDAAFARTQQVEEQVSTLDQRIGRAVASIRDVLDGSV"
     gene            2531..2899
                     /gene="zapA"
                     /locus_tag="PK812_05755"
     CDS             2531..2899
                     /gene="zapA"
                     /locus_tag="PK812_05755"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HRJ69097.1"
                     /translation="MSQVSVSIDGKMFRMACEDGQEAHLEALAKHLDVRIGEMRQAFG
                     EIGDLRLTVMAAITITDEFTEARKKAEDLTQQIGHLRNAQVSAQDNAGARDQEIAGLI
                     DRMTQRLERIAGLLSQRPEM"
     gene            2947..3103
                     /gene="ssrS"
                     /locus_tag="PK812_05760"
     ncRNA           2947..3103
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PK812_05760"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            3123..3650
                     /locus_tag="PK812_05765"
                     /pseudo
     CDS             3123..3650
                     /locus_tag="PK812_05765"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
     gene            3708..4523
                     /locus_tag="PK812_05770"
     CDS             3708..4523
                     /locus_tag="PK812_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HRJ69098.1"
                     /translation="MRILFLGDICGRPGRTIVTQQVPLLRQRWKLDAVIANGENAAAG
                     FGITEAILHEFLECGIDAVTLGNHSWDQREALVFIDRQPRLVRPANYPAGTPGRGATL
                     IETASGARVLVVNLLGRLFMEAFDDSVRAVERELEACPLGQVADAIVVDMHCEATSEK
                     QVMGHMLDGRVSLVVGTHTHTPSADARILSGGTAFQTDTGMCGDYAGVIGFDRREPVR
                     RYIERTPGGRWESASGPGTLCGIAIETDDRTGLAVSAARVCIGPELENCGPAW"
     gene            4528..>4883
                     /locus_tag="PK812_05775"
     CDS             4528..>4883
                     /locus_tag="PK812_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018429528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HRJ69099.1"
                     /translation="MTPDAYNAFCASLPATTYVCQWGGSHVWKVGGKVFAIGSWDEPY
                     AGITVKVSDMAFEILPTQPGLRPAPYLASRGMKWIQHYSAPGLSDDDLTLYLRESHRI
                     VASGLSRRRRADLGLP"
     assembly_gap    4884..4983
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4984..5157
                     /locus_tag="PK812_05780"
     CDS             <4984..5157
                     /locus_tag="PK812_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 family protein"
                     /protein_id="HRJ69100.1"
                     /translation="GEVVVFSWITYRSRRHRDAVNARVMADPRMKALMDPATMVFDGK
                     RLIYGGFRTMIAL"
     gene            5385..6167
                     /locus_tag="PK812_05785"
     CDS             5385..6167
                     /locus_tag="PK812_05785"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HRJ69101.1"
                     /translation="MAIGLTLLDDTYIMASADRLYAFRGNDTVTGSAGNDTILGGRGD
                     DVLYGMDGDDLIFGGADNDYIDGGNGDDILRGDGGADTLIGGTGEDDLNGGAGDDFVD
                     GGEDHDVVRGGAGHDVLSGGSGDDLVHGGLGNDFMDGGIGNDYMFGDFGADTMMDGDG
                     SDWLHGGAGNDRFNLAADTSFGTLDVIHGGTGIDTAWFAPQNGSVTVTNMGGWFDVTW
                     TSGVGDPQHDQLFGVEFLLVEDVATGIVETVDLATVAVVWTT"
     gene            6342..7139
                     /locus_tag="PK812_05790"
     CDS             6342..7139
                     /locus_tag="PK812_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRJ69102.1"
                     /translation="MASATLVSIGEDHLLFDAGRGVMVQAVKAGVPLAAIGPVFITHH
                     HFDHIGDLFDVFLNSWMAGRRAPLRVFGPPGTQGIVEALTTQVYDRDIAWRSLGEPTF
                     GGWAPVETTDIAAGPVLDTGRWRVSAEIVRHGDGLGFPDAFLRRWTCLGYRIEAEGKV
                     VAISGDTVDCEGLRRLAHGADVLVQVCFLARSEIRGAHFERLARHTLACADTVGGIAA
                     ECGVKTLILTHHRPRPDDGLLDEIRADVARDFAGELIIGQDLTRIDL"
     gene            7232..7978
                     /locus_tag="PK812_05795"
     CDS             7232..7978
                     /locus_tag="PK812_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRJ69103.1"
                     /translation="MAGHSQFKNIMHKKGKQDKLRSKVFSKLAREITVAAKMGLPDPA
                     FNPRLRSAILAARAENMPKDNIERAIKKASGGDSETYDEIRYEGYGPGGTALIVEVMT
                     DNRNRTASDVRSYFTKAGGNLAETGAVSFMFDRVGLVEFDADKASADAMLEAAIEAGA
                     DDAVSGESGHEVYCEQSSLAEVSRALEARFGEPRKSKLVWRPQTLISVDDEAGETLLG
                     LMEKLEEHDDVQNVYANFEISDALMAKMGS"
     gene            8177..9004
                     /locus_tag="PK812_05800"
     CDS             8177..9004
                     /locus_tag="PK812_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HRJ69104.1"
                     /translation="MDAQDALARDVPAEEPPRRPDPAIRSISFKDIGEALAAGLRDLQ
                     AAPVYGLTFGALYCLGGILIVLFVTALGMPYFAYPLAAGFALLGPMVAIGLYEVSRLR
                     EMGAVLSWGAVLRAMKAQARQEIGWMSFVVLFVMIIWLYQVRLLLALFLGFEPISTLR
                     DFLTVVLTTPQGWMFLAIGHVIGAVLSLVLFSLTVVSFPILLDRPVDFITAMITSVRS
                     VVTNPQPMVAWAIMIVALLICATLPMFLGLIVVLPILGHATWHLYRRLVAPEQPAAT"
     gene            complement(9058..9750)
                     /locus_tag="PK812_05805"
     CDS             complement(9058..9750)
                     /locus_tag="PK812_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1013 domain-containing protein"
                     /protein_id="HRJ69105.1"
                     /translation="MATLLMPKATAVWLVENTSLTFDQIADFCKLHPLEVKGIADGEV
                     ATSIKGFDPVLGGQLAREELERAGSNPNYRMRVLEPKVRVPEMKRTKGPRYTPVSKRQ
                     DRPNAILWLLRNHPELKDAQIMRLVGTTKTTILSVKERTHWNATNLAPMDPVTLGLCS
                     QMDLDFEVNRAAKERPAPVETGATLLPASVTTAPAAEEPAPRSAAAAKREEEAEIASV
                     FAKLKELKGSDE"
     gene            complement(9887..10834)
                     /locus_tag="PK812_05810"
     CDS             complement(9887..10834)
                     /locus_tag="PK812_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRJ69106.1"
                     /translation="MKDIDHFALDGQSLRLFLQVLEEGSVTGAADRLGVTQSAVSHGL
                     QKLRRILGDPLFVKNGRGIVATAHALSLADKARRLVDELEAFASPPGFDPATARLSLT
                     IAANDFQSELLLPAVFRTLSAAVARVDLRITPSGSPTPDLLRDRRCDLILTPVPPEGT
                     DILRRRLFEDRYLCFFDGTRRAAPRDFAAYGAARHVTVVHPDGERLEFDKKLDARGIR
                     RDIIIRVQGFAGVPSFLRGTDMLASLPGLLARNLMRAFSSAPVPLDSEAAGDAAILPM
                     YMVWHRRDHLDPAHAFVRDVVVEAAQEARYPRPALPPKQ"
     gene            10921..11646
                     /gene="pcaH"
                     /locus_tag="PK812_05815"
     CDS             10921..11646
                     /gene="pcaH"
                     /locus_tag="PK812_05815"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HRJ69107.1"
                     /translation="MTRTGEFIRRDRLWHPPQYQPGYKTSVLRSPRRALVSLEQSSSE
                     LTGPKFGHTSLDPLDNDLIRNYATSGEAIGERIVIHGRVLDECGRPVPHTLLEVWQAN
                     AGGKYRHINDGYMAAMDPNFGGVGRCLTDENGHYYFRTIRPGPYPWRNNGSDWRPAHI
                     HFSIFGEAFAQRLITQMYFEGDPLIPLCPIVNTIPDKRAIDMLVARLDMGEMMPFDSL
                     AYRFDIVLRGRRSTFFENKPEGN"
     gene            11648..12238
                     /gene="pcaG"
                     /locus_tag="PK812_05820"
     CDS             11648..12238
                     /gene="pcaG"
                     /locus_tag="PK812_05820"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HRJ69108.1"
                     /translation="MPVEYLKETPSQTAGPFLHIGMLPAQAGLRTRSQEKMNVMVSGD
                     VEGERIRVEGIVYDGSGAMVRDAQVEIWQANAKGVYNAPGFLGFGRAGADFKTGEWFI
                     ETIKPGRTPGRKGKLQAPHLTIYVYARGINIHLHTRMYFEDEAEANAADPVLNAIELK
                     HTVATLIAKRSKRGGETVYRFDIHLQGEKETVFFDI"
BASE COUNT         1998 a         4028 c         4136 g         2079 t
ORIGIN      
        1 gtctggcgcg gcgccggcat cggcctgttc atccaggcct cattgatgct ggcgctcgat
       61 ttcttcgccg agcggcgcgg cgcggcgtat ctggactggc tggcgaaaca ggcgtagcgc
      121 ggccgccggg gcagccgcgt ccagctgcct tatttcccca gcgccttgcc gatttcctcc
      181 agcgtcgccg gatcgtcgat ggtcgccggc atggaccagg cgtcgccgtc ggcgattttc
      241 ttcatggtgc cgcgcaggat cttgccggag cgggtcttcg gcaggcggtt gaccaccacc
      301 gtctgcttgt aggccgcgac ggggccgatt ttctcgcgca ccagcgcggc gcattccttt
      361 tcgacctcgg cggcggtctt ggtcacgcct gccttcagca cgacgaagcc gagcggtgcc
      421 tcgcccttca gcgaatcctt gatgccgacc acggcgcatt ccgccaccga cgggtgcgag
      481 gacagcacct cctccatgcc gccggtggag aggcggtgac cggcgacgtt gatgatgtcg
      541 tcggtgcggc ccatgatgta gaggtagccg tcctcgtcgc gatagccggc atccgaggtg
      601 gagtaatatc ccttgaactc ggtaagatag ctctgctcga atcggtcgtc cgcctgccag
      661 agcgtcggca gcgcacccgg aggcagcggc agcttgatgg cgatcgagcc catcttgccg
      721 tcgggcacgg gtttgccgcc ctcgtcgagg atctgcacgt cgtagcccgg catcggcacc
      781 gtcggggagc cgcgcttcac cggaagcaac tcgattccgc gcgggttgcc ggcaatgcac
      841 cagccggtct cggtctgcca ccagtggtcg atcaccggca cgccgagaat gtcctccgcc
      901 catttcaccg tatcggggtc ggcgcgttcg cccgccaggt agagcgcctg aaggcaggag
      961 atgtcgtagg tcttcaggaa cttggcctcc gcgtcttcct tcttgatggc gcggaaggcg
     1021 gtcggagcgg tgaagaacgc cttgaccttg ttctccgaga tcacgcgcca gaaggcaccc
     1081 gggtccggcg tccccaccgg cttgccctcg tagacgatgg tggtgcagcc cttcagcagc
     1141 ggcgcatagc agatgtagga gtggcccacg acccagccga cgtccgaggc ggcccagaag
     1201 gcatcgcccg cgtcgacgcc gtagagcgcc ttcatcgacc agtgcagcgc caccatgtgg
     1261 ccaccgttgt cgcgcaccac gcccttcggc ttcccggtcg tgccggaggt gtagagcaca
     1321 tagagcgggt ccgtggcctg catcggctcg caggcggagc ctgcggcctt gccggaggcg
     1381 cgcatggcgt cgcgcgcggc agcccagtca tgatcccggc cggcctgaag ggcggcggtt
     1441 tccatcggcc gctggaagat cagacaggcg gcgggcttgt tcgccgaaag ggaaatcgcc
     1501 tcgtccagca agggcttgta cttgacgata cggccaggct cgatgccgca ggaggccgag
     1561 aggatgagct tgggcgcgca atcgtcgatg cgggttgcca gttcccgcgc cgcgaagccg
     1621 ccaaacacga cggagtgcac cgcgccgacg cgcgcgcagg ccagcatgcc gatcaccgct
     1681 tcggggatca tcggcatgta caggaggacg cggtcaccct tcttcacgcc gaagccacgg
     1741 atgatctcgg ccagcacctt cacctcgtcg gtgagttcag cgtagctcag cgaagccttg
     1801 gagttggtga caggggaatc atacacaagc gccgcctggg cgccgcgtcc gccggcgaca
     1861 tgccggtcga ggcagttgtg ggcggtgttg cagacgccgt ccgggaacca gcggccgtag
     1921 acgccggcct tggcgtcgaa cacgaccttc gggacggtga accagtccag cgacttcgcg
     1981 gcttcgcccc agaaggcctg cgggtcattc ttccatcccg cgtagagcgc ggcgtatgcc
     2041 cctttgccca tgatctgact ccccatttgg cggtttatgc ggcggatttg tcattttttg
     2101 cggtcatggc gcaagcggag ccacctcatc acttcgatga acggccgcgc ccgaaagcgg
     2161 tgcgtaacct gccgcgcgca ttgacgcccg cttcgcgcgg tgtctaacct tccgtttcta
     2221 acgccttgat cgcaaagccg agtcgtcttt caggcatgag ccgggaaacc atcctcgaag
     2281 cgttggcgcg gtgcggcgcc gccttggatc ggctggaggc cgcgtcactg cgccaccagg
     2341 agggcgaacg gctgcgcatg aatctggaga gcgaactggc gctgatgcgc gcggatcggc
     2401 aggcgctggc cgagaagctc gacgcggcgt tcgcccggac ccagcaggta gaagagcagg
     2461 tctcgacgct ggaccagcgg atcggccgtg cggtcgcctc catccgcgac gtcctggacg
     2521 ggagcgtctg atgtcgcagg tctcggtctc gatcgacggc aagatgttcc gcatggcctg
     2581 cgaagatggt caggaggccc atctggaggc cctggccaag catctcgacg tgcgcatcgg
     2641 cgaaatgcgg caggcattcg gggagatcgg cgacctgcgg cttaccgtca tggcggccat
     2701 caccatcacc gacgaattca ccgaggcccg caagaaggcc gaggacctga cgcagcagat
     2761 cggccacctg cgcaatgccc aggtctctgc gcaggataac gccggcgcgc gggaccagga
     2821 gatcgccggg ctgatcgacc ggatgaccca gcggctggag cgtatcgccg gattgttgtc
     2881 gcaaaggccg gaaatgtgaa cggccggctc tggcgaggcg acccgaacag gtctacatta
     2941 cgcggtgcgg ggctgcggga tgcgtcagga gacacgtatc cccggggcct tatcgatcct
     3001 aagggagctg tccctggctg gactcctggg tccggacaca cggcgcccac ctactcacgt
     3061 aggttcccgg gatcgacact ccaacggtca tcgcggctcc gcaccctttc tcttttcagg
     3121 ccatgaccag cgccgcagag tccaaggaag cgctgcgccg cgccatgcgc cgccagcgcg
     3181 ccgaagtacc cgtagaacag cgccggctcg cgtcagaaac gctcacgaac gcgtcccgaa
     3241 gcctcttgct cgggctcagt gcgccgatcg tttcgggcta tgtcccgatc cgggcggaga
     3301 tcgaccccgg cgcgctgatg gcgtcgatgg aacggtacgg cgcgcggctg gccctgccgc
     3361 gtgtggaggg tgatcgtctc gcgttccggt tgcagctgcc cggcacgccg ctcgtgccgg
     3421 gagcctatgg catcggcgaa ccggcagccg ggtcgccggt cgtcgtgccg gatctcatgc
     3481 tggtgccgct gctggccttc gaccggcgcg gcgggcggct cggctacggc gcgggctatt
     3541 acgaccgcgc gatcacgacg atgcagcccc ggctgaccgt cgggctggcc ttcgcctgtc
     3601 aggaagtcgc agcggtgccg atggaaacgc atgaccggcg gctcgatctc atcctgaccg
     3661 aacaggggct gattgactgc cggcgggggg cggcgcatag caggtccatg cgcatcctgt
     3721 ttctcggcga catctgcggc cgtccgggcc ggaccatcgt gacccagcag gtgccgctgc
     3781 tgcgccagcg ctggaagctc gatgccgtga tcgccaacgg cgagaatgcg gcggccggct
     3841 tcggcatcac cgaggcgatc ctgcacgagt tcctcgaatg cggcatcgat gcagtgaccc
     3901 tgggcaacca ttcctgggac cagcgcgagg cgctggtgtt catcgaccgc cagccgcgcc
     3961 tggtgcgtcc ggcgaattat ccggcgggaa cgcccggccg cggggccacg ctgatcgaga
     4021 cggcgagcgg cgcccgtgtg cttgtggtga acctgctcgg ccggctgttc atggaggcct
     4081 tcgacgattc ggtccgcgcg gtggaacgcg agcttgaggc ctgcccgctc gggcaggtcg
     4141 ccgatgccat cgtcgtcgac atgcattgcg aggcgaccag cgaaaagcag gtgatggggc
     4201 acatgctgga cggccgcgtc tcgctggtgg tgggcacgca tacccacacg ccgtcggccg
     4261 acgcgcgcat cctttccggc ggcaccgcct tccagaccga tacgggcatg tgtggcgatt
     4321 atgccggcgt catcggcttc gaccggcgcg agccggtgcg ccgctacatc gaacgcacgc
     4381 cgggcgggcg ctgggaatcg gccagcgggc cgggcacgct ctgcggcatc gcgattgaaa
     4441 cggacgacag gaccgggctc gcggtctcgg ccgcacgggt ctgcatcggc ccggagctgg
     4501 agaactgcgg gccggcgtgg tgaggtgatg acgcccgacg cctacaacgc cttctgcgcg
     4561 agcctgccgg cgacgaccta cgtctgccag tggggtggct cgcatgtgtg gaaggtcggc
     4621 ggcaaggtgt tcgccatcgg cagctgggac gagccctatg ccggcatcac cgtcaaggtc
     4681 tcggacatgg cgttcgaaat cctgccgacg cagccgggcc tgaggcccgc gccctatctc
     4741 gcatcgcgcg gcatgaaatg gatccagcac tactccgcgc cgggcctgag cgatgacgac
     4801 ctgacgctct acctcaggga gtcgcaccgc atcgtcgcat ccggactgtc acggcggcgg
     4861 cgcgcggacc tcgggttgcc ggannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnggcgagg tcgtcgtgtt ttcctggatc acctaccgct cgcgcaggca ccgcgacgcg
     5041 gtgaatgcca gggtcatggc cgatccgcgc atgaaggcgc tgatggaccc ggcgaccatg
     5101 gtgttcgacg gcaaacgcct catctacggc ggcttcagga cgatgatcgc gctgtagccc
     5161 ggggcaggcg ggcgtcacgc gcggcttgtg cgccagcgcg cagacaaatg ctccgccgct
     5221 gttaacgtcg cgtttgccat tcgagacggc acaatcgggc gcccttgccc ggtccgccgt
     5281 ttcagtgcgt tgccacacat cccaactccg atcccggtgc gatacctcac gtcgcgtcct
     5341 gggggcaggc gcttctcgca acacctgctc tcaggagtat attcatggct atcggtctca
     5401 cccttcttga cgacacctac atcatggctt ccgccgaccg cctctatgcg ttccgcggca
     5461 acgacacggt gaccgggtcc gccggcaacg atacgatcct cggcggtcgc ggcgacgacg
     5521 tgctctacgg catggacggc gacgacctga tcttcggcgg cgccgacaac gactacatcg
     5581 acggcggcaa cggcgacgac atcctgcgcg gcgatggcgg agcggacact ctcatcggcg
     5641 gcaccgggga agacgacctg aacggcggcg cgggcgatga cttcgtcgac ggcggcgaag
     5701 accacgatgt ggtgcgcggc ggcgccggcc acgatgtgct gtccggtggc tccggcgacg
     5761 acctggtcca tggcggcctg ggcaacgact tcatggatgg cggcatcggc aacgactaca
     5821 tgtttggcga cttcggcgcc gacacgatga tggacggcga cggttcggac tggctgcacg
     5881 gaggcgccgg caacgaccgc ttcaatctgg ccgccgacac atctttcggc acgctcgacg
     5941 tcattcacgg cggcaccggc atcgacacgg cctggttcgc gccgcagaac ggttccgtca
     6001 ccgtcaccaa catgggcggc tggttcgacg tgacctggac cagcggcgtc ggcgatccgc
     6061 agcatgacca gctgttcggc gtcgagttcc tgctggtgga ggatgtcgcg accggcatcg
     6121 tcgagaccgt cgacctggcg accgtcgccg tggtctggac gacctgagcc cagccgcgaa
     6181 cgcggttttc ttcacggacc ccggcgggag accgccgggg ttcttcgtcc tggttgacgc
     6241 cgtgtcggca gcgttcgaga ctgcttccca tcggaacagg accctttcgc ttgcgcatca
     6301 cgcttcttgg aaccggcggc ccgagaccgg acccgcgccg catggccagc gcgacgcttg
     6361 tctccatcgg cgaggaccac ctgctgtttg acgccgggcg cggcgtgatg gtgcaggcgg
     6421 tcaaggccgg cgtgccgctg gcggcgatcg ggccggtgtt catcacgcat caccatttcg
     6481 atcacatcgg cgatctgttc gatgtcttcc tcaactcctg gatggccggc cggcgcgcgc
     6541 ccctgcgcgt cttcggtccg ccgggtacgc aaggaatcgt cgaggcattg acgacccagg
     6601 tctacgatcg cgacatcgcg tggcgctcgc tcggcgaacc gaccttcggc ggctgggcgc
     6661 cggtggagac gaccgacatc gcggccggac cggtgctgga taccgggcgc tggcgggtga
     6721 gcgccgagat tgtgcgccat ggcgacgggc ttggctttcc cgatgctttc ctgcggcgct
     6781 ggacctgcct cggctaccgc atcgaggccg aaggcaaggt tgtggcgatt tccggcgaca
     6841 ccgtcgactg cgagggcctg cggcggttgg cgcatggcgc ggacgtgctc gtgcaggtgt
     6901 gcttcctggc ccgcagcgaa atccggggcg cacacttcga acggctggcg cgccacacgc
     6961 tggcctgcgc cgacacggtg ggcggcatcg cggcggaatg tggcgtcaag acgctgatcc
     7021 tgacccatca ccgtccgcgt cctgacgacg ggctgctcga tgagatccgc gccgacgtcg
     7081 cccgcgactt cgccggcgaa ctcatcatcg ggcaggacct gacccgcatc gatctctgaa
     7141 tggcgcggcg gtttgctttc cgcgcgccag cggctatagg cggacatccc tatcgcgaag
     7201 agcaagaggc ggccgcaagg ccgttggcgc catggccgga cacagtcagt tcaagaacat
     7261 catgcacaag aagggcaagc aggataagct gcgctcgaag gtgttctcca agctggcgcg
     7321 cgaaatcacc gtcgcggcca agatggggct gcccgatccg gccttcaatc cgcgcctgcg
     7381 ctcggccatt ctggcggccc gggccgagaa catgccgaag gacaatatcg agcgcgccat
     7441 caagaaggcc tccggcggcg attcggagac ctacgatgaa atccgctacg agggctacgg
     7501 gccgggcggc accgcgctga tcgtcgaggt gatgaccgac aaccgcaatc gcacggcctc
     7561 ggacgtgcgg tcctatttca ccaaggccgg cggcaacctg gcggagaccg gcgccgtgtc
     7621 gttcatgttc gaccgggtcg ggcttgtcga gttcgacgcc gacaaggcca gcgcggacgc
     7681 catgctggaa gcggcgatcg aggcgggcgc ggacgatgcc gtctccggcg agagcgggca
     7741 cgaggtctat tgcgagcagt cgagcctcgc cgaagtgtcc agggcgctgg aggcgaggtt
     7801 cggcgaaccg cgcaagtcga agctggtctg gcggccgcag acgctgattt ccgtcgacga
     7861 cgaggcgggc gagacattgc tcggcctcat ggaaaagctc gaagagcatg acgacgtgca
     7921 gaacgtctac gccaacttcg agatttccga cgcgctcatg gccaagatgg gaagctgatc
     7981 cggcggacct gcccctgacg tgaccatcgc cgccggctcc tgacgcggtc gccgggcctt
     8041 ggccctgccc gggatcagac ggcccgcgcg aggcgcggca tttcgctgct ttgacagtcc
     8101 ggcgcacttt gacaaagatc aaaggcaaac cgcacagtca gcgtgaccgt aaagagacag
     8161 gcagggaggg tttgccatgg acgcgcagga cgcgcttgcc agggatgtgc cggccgagga
     8221 accgccgcgc cggccggatc cggcgatccg cagcatcagc ttcaaggaca tcggggaagc
     8281 cctcgcagcc ggtctgcgcg atctccaggc cgcgcctgtc tacggcctga ccttcggcgc
     8341 gctgtactgc ctcggcggca tcctgatcgt gcttttcgtc acggcgctcg gcatgccgta
     8401 ttttgcctat cccctggccg cgggctttgc cctgctcggc ccgatggtgg cgatcggcct
     8461 ctacgaggtc agccggctgc gcgagatggg tgcggtgctc agctggggcg cggtgctgcg
     8521 cgccatgaag gcgcaggcgc gtcaggaaat cggctggatg agcttcgtcg ttctgttcgt
     8581 catgatcatc tggctctacc aggtgcgcct gctgctggcg ctgttcctgg ggttcgagcc
     8641 gatctcgacg ctgcgcgatt tcctcacggt ggtgctgacg acgccgcagg gctggatgtt
     8701 cctcgccatc gggcatgtga tcggcgccgt cctctcgctc gtcctgttct cgctgacggt
     8761 ggtgtcgttc ccgatccttc tggaccggcc ggtcgatttc atcaccgcga tgatcaccag
     8821 cgtgcgctcg gtggtgacca atccgcagcc catggtcgcc tgggccatca tgatcgtggc
     8881 gctgctgatc tgcgcgacgc tgccgatgtt cctggggctg atcgtcgtgc tgccgatcct
     8941 cggccatgcc acctggcatc tctaccggcg gctggtcgcg ccggaacagc cggccgcgac
     9001 atgaaaaaag gccgggatcg ctcccggcct tcgtcagcat ggcgggcgaa cgggcggtta
     9061 ctcgtcgctg cccttgagct ccttgagctt ggcgaagacg gaggcgatct ccgcctcttc
     9121 ttcccgcttc gccgcggcgg ccgagcgggg agccggctcc tccgccgccg gagcggtggt
     9181 gacggatgcc ggcagcaggg tcgctccggt ttcgaccggg gccgggcgct ccttggcggc
     9241 gcggttcact tcaaagtcga gatccatctg cgagcacagc cccagcgtca ccggatccat
     9301 cggcgccagg ttggtggcgt tccagtgggt gcgctccttc accgaaagga tggtcgtctt
     9361 ggtcgtgccg accagacgca tgatctgggc gtccttcagt tcgggatggt tgcgcagcag
     9421 ccagagaatg gcgttcggcc ggtcctggcg cttggaaacc ggcgtgtagc gcgggccctt
     9481 ggtgcgcttc atctccggca cgcgcacctt cggctccagc accctcatgc ggtagttcgg
     9541 gttgctgccg gcgcgctcga gctcctcgcg ggcgagctgg ccgccgagca cgggatcgaa
     9601 ccccttgatg ctcgtggcga cctcgccatc ggcgatgccc ttcacttcaa gcgggtgaag
     9661 cttgcagaaa tcggcgatct gatcgaaggt cagagacgtg ttttccacca gccatacggc
     9721 ggtcgccttc ggcatcagaa gcgtggccat gtgacatcct ccagaacagc gcagctcgcg
     9781 ggcgcttcgg gcaatgcttc ttcgctttcc gaaacaggga agcgacagca cgagcaccat
     9841 ttgacgggaa tccgcgcctt atactgtgca tcggcgcgac tggcaatcac tgctttggcg
     9901 gcagtgccgg acggggatag cgcgcctcct gcgccgcctc gaccaccacg tcccgcacga
     9961 aggcgtgcgc cgggtcgagg tggtcgcgcc ggtgccagac catgtacatc ggcagtatcg
    10021 ccgcgtcacc cgccgcttcg gagtccagcg gtaccggcgc ggatgaaaac gcccgcatca
    10081 ggttgcgtgc cagcaggccc ggcaggctgg cgagcatgtc ggtgccgcgc aggaagctgg
    10141 gcacgccggc aaagccctgg acccgaatga tgatgtcgcg gcggataccg cgcgcatcga
    10201 gcttcttgtc gaactccagg cgctccccgt cgggatggac cacggtgaca tgccgcgcgg
    10261 cgccataggc ggcgaaatca cgcggcgcgg cgcggcgcgt tccatcgaag aagcagagat
    10321 agcgatcctc gaacagccgc cggcgcagga tgtccgtccc ctcgggcggc accggcgtga
    10381 gaatgagatc gcagcgccgg tcccgcagca ggtccggcgt gggggagccc gagggcgtga
    10441 tgcgcaggtc gacccgcgcg accgccgcgg acagggtgcg gaagacggcc ggcagcaaca
    10501 gttcggactg gaagtcgttg gcggcgatgg tcagcgacag gcgggcggtc gccggatcga
    10561 agcccggcgg actggcgaac gcctccagtt cgtcgacgag gcggcgcgcc ttgtccgcca
    10621 gggagagcgc gtgcgcggtg gcgacgatgc cccgaccgtt cttcacaaac agcggatcgc
    10681 cgaggatccg ccgcagcttc tgcaatccgt ggctgaccgc cgactgggtg accccgagcc
    10741 tgtcggccgc tccggtgacc gatccctcct ccagaacctg gaggaacagt cgcaggctct
    10801 ggccgtccag cgcaaaatga tcgatatcct tcatggatca tatcataatt gaacgcttta
    10861 tttgttcaat tgccggggcg atggtctggc acgtccagag cgatccttgg gaggaacccg
    10921 atgacccgaa ccggagagtt catccggcgc gaccggttgt ggcatccgcc gcaataccag
    10981 cccggctaca agacctcggt tctgcgctcg ccgcgccgcg ccctggtgtc gctggagcaa
    11041 tcgtcgagcg agctgaccgg accgaagttc ggccacacca gcctcgatcc gctcgacaac
    11101 gacctgatcc gcaactacgc gacctcgggc gaggcgatcg gcgaacgcat cgtcatccat
    11161 ggccgcgtgc tcgacgaatg cgggcgaccg gtgccccaca cgctgcttga agtctggcag
    11221 gccaatgcag gcggcaagta ccgccacatc aacgatggct acatggcggc gatggacccg
    11281 aatttcggcg gcgtcggccg ctgcctgacg gacgagaacg gccattacta tttccgcacc
    11341 atccgtcccg gcccctatcc ctggcgcaac aacggcagcg actggcgccc cgcccacatc
    11401 catttctcga tctttggcga ggccttcgcc cagcgcctga tcacccagat gtatttcgaa
    11461 ggcgatccgc tgatccccct ctgcccgatc gtcaacacga tcccggacaa gcgggcgatc
    11521 gacatgctgg ttgccaggct cgacatgggc gagatgatgc ccttcgactc gctggcctac
    11581 cggttcgaca tcgtgctgcg cggtcgccgg tccacgtttt tcgagaacaa gccggagggg
    11641 aactgacatg cccgtcgagt atctgaagga aacgccatcc cagacggctg gtcccttcct
    11701 gcatatcggc atgctcccgg cgcaggccgg actgcggacg aggtcgcagg aaaaaatgaa
    11761 cgtcatggtt tcaggcgacg tggaaggcga gcgcatccgc gtcgagggca tcgtctatga
    11821 cggctcgggc gcgatggtgc gcgatgcgca ggtcgagatc tggcaggcca acgcgaaggg
    11881 cgtctataac gcgcccgggt ttctcgggtt cggccgcgcc ggcgccgatt tcaagacggg
    11941 cgagtggttc atcgagacca tcaagccggg gcggaccccg ggtcgcaagg gcaagctgca
    12001 agcgccgcac ctcaccatct acgtctacgc gcgcggcatc aacatccacc tgcacacgcg
    12061 gatgtacttc gaggacgagg cggaggcgaa cgctgccgac cccgtgctga acgccatcga
    12121 gctcaagcat acggtcgcga ccctgatcgc caagcgctcg aagcggggtg gcgagacggt
    12181 ctatcgcttt gacatccatc ttcagggcga gaaggaaacg gtgttcttcg acatctgata
    12241 gcctgccatg tgacgcgatg cagccggcgg gcgacgatga acgctctcgc cacgtcccgc
    12301 cggacctgct agctagcgcc cccgacagag gggtgcatga g
//
LOCUS       DAOMXN010000051        12095 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_2853_length_12095_cov_11.711296, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000051 DAOMXN010000000
VERSION     DAOMXN010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 12095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12095
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2853_length_12095_cov_11.711296"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /gene="narJ"
                     /locus_tag="PK812_05825"
     CDS             complement(<1..613)
                     /gene="narJ"
                     /locus_tag="PK812_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HRJ69109.1"
                     /translation="MMKTLKVLSALLTYPSPDLVEAIGDMRFVIDREGVLPARVAAAV
                     HRLMDHLADGDIYDRQEAYGLLFDRTRSLSLHLFEHVHGESRDRGQAMVDLIKVYEEN
                     GYSPVTTELPDYLPLFLEYASTRPAKEAIDLVGQPAHVITALKERLVKRSSPYAAVMD
                     AVLALAKARPDEAALAALRAEPDPEPDDLEALDAAWEEEEVRFG"
     gene            complement(610..2130)
                     /gene="narH"
                     /locus_tag="PK812_05830"
     CDS             complement(610..2130)
                     /gene="narH"
                     /locus_tag="PK812_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HRJ69110.1"
                     /translation="MKIRAQIAMVLNLDKCIGCHTCSVTCKNVWTNREGMEYAWFNNV
                     ETKPGIGYPKEWENQTRWKGGWKRTASGKIEPKIGGKWRILSQIFANPNLPEIDDYYE
                     PFTFDYEHLQKAPELEAFPTARPRSLITGERMEKIHWGPNWEEILGGEFSKRSKDANF
                     EGVQKEIYGQFENTFMMYLPRLCEHCLNPACVASCPSGAIYKRAEDGVVLIDQDKCRG
                     WRMCVSGCPYKKIYFNWSSGKSEKCILCYPRLESGQPTVCSETCVGRIRYLGVILYDA
                     DKIEAAASEKSERNLYDSQLKVFLDPNDPAVIAEARKQGIPEAWLESAKQSPIWKMAM
                     EWKVAFPLHPEYRTLPMVWYIPPLSPISHAAEAGKIGVDGDMPDVRNLRIPLRYLANL
                     LTAGDEEPVAKGLERMLAMRAYMRAKTVDGVIDEGIARKVGLTGLQIEEMYHIMAIAN
                     YEDRFVIPTTRREVAEDAYGLRGSCGFSFGNGCSDGETPTNLFGGKPKAKNPMEMA"
     gene            complement(2146..5892)
                     /locus_tag="PK812_05835"
     CDS             complement(2146..5892)
                     /locus_tag="PK812_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HRJ69111.1"
                     /translation="MSHFLDRLTFFSKLREPFSNGHGVATSEDRAWEEGYRKRWQHDK
                     VVRSTHGANCTGSCSWKIYVKGGIVTWETQQTDYPRTRPDLPNHEPRGCSRGASYSWY
                     LYSGNRVKYPLVRSRLLKLWRAARATLTPVAAWKSIVEDPGKRASYTKKRGHGGFVRA
                     SWDEVTEIIASANAYTAKTYGPDRILGFSPIPAMSMVSYAAGSRYLSLIGGVCMSFYD
                     WYCDLPPSSPQTWGEQTDVPESADWYNAGFIILWGSNVPQTRTPDAHFYTEARYRGTK
                     SAVICPDYSEASKFGDIWLSVKQGTDAALAMAMGHVILREFHVDRQAQYFRDYVRQYT
                     DMPMLVKLVQQDGKLVPDRFLRASDIKGALGETNNPEWKTLAFDEKSGKLVAPTGSIG
                     YRWGETGKWNLEEKASNGDATTLRLSLAEVKDGIEDVAFPYFGNIKNEHFVSDSHASV
                     LNRKVPVKKVKTTDGDVLVASVYDLFLANYGVDRGFGGANVAKSYDEDVPYTPAWAEK
                     ITGVTRDKIITVAREFALNAEKTKGRSMVIIGAAMNHWYHADMNYRGVINMLVMCGCV
                     GQSGGGWSHYVGQEKLRPQSGWLPLAFGLDWGRPPRQQNSTSCWYAHTDQWRYEQVKI
                     GEIVSPTAPAGPWDGAIIDYNIRSERMGWLPSAPQLQTNPLQVSKDAAAAGMEAKDYV
                     AAKLKSGELKMSCEDPDNPKNWPRNLFVWRSNLLGSSGKGHEYFLKHLLGTSHGVMGK
                     DLGEQGHAKPLEAVWHDKAPEGKLDLLVTLDFRMSTTCVYSDIVLPTATWYEKNDLNT
                     SDMHPFIHPLTNAVDPAWQSRSDWDIYKSIAKKFSDVAPEVLGVEKDVVLNPIMHDSP
                     NEIAQALDVKDWKKGECEPIPGKTMPTVAVVERDYPNTYKRFTSLGPLMEKIGNGGKG
                     MAWNTAHEVEFLKALNGVVTEDGPTKGLARIETDIDASEVILSLAPETNGEVAVKAWK
                     ALSKTTGRDHTHLAIPKEDEKIRFRDVVAQPRKIISSPVWSGLESEKVCYNAGYTNVH
                     ELIPWRTLTGRQQLYQDHLWMRAFGEALCVYRPPVDLKTIAPVKDLKPNGEKEILLNF
                     ITPHQKWGIHSTYTDNLMMLTLSRGGPIVWLSEKDAAKAGIVDNDWVEAYNANGALVA
                     RAVVSQRMKEGTLFMYHAQEKIVNVPGSEMTGQRGGIHNSVTRAIPKPTHMIGGYAQQ
                     SYGFNYYGTIGTNRDEFVVVRKVRNVDWLERPAKDAEPTIQAAE"
     gene            complement(5917..7281)
                     /locus_tag="PK812_05840"
     CDS             complement(5917..7281)
                     /locus_tag="PK812_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HRJ69112.1"
                     /translation="MANADTSASTARGASWLTRWEPENETFWKEGGSSRAWLTLWITT
                     ANLIMAFIVWFVVSALVVRLPQIGFKLTTSQLFWLAAMPGLAGGTLRLIHMFLTPMYG
                     TRAVVSLSTLSLLIPAVGWFYAVQDPTTPYWMLMLLAFLAGLGGGNFSSFMPSTSMFF
                     PKRLQGTALAIQAGIGNFGVSVVQFITPWIITVALMGGATWMGGNQTMTVAGAQKSIW
                     LQNATAIYIPFIAVFGISAWLLLKSVPIKANFAEQFDIFRSRHTWSMTSLYIMTFGAF
                     SGLSGTFPLLIKQCYGALPGAPDPLAFAFYGPLVGSISRIIAGPISDKLGGARVTHWA
                     GLGMLICAAVVPMYTQPTSMASFPYFVGAMLGLFFFAGIGNASTFKQMPMIFPPRQAG
                     GVIGFTGAIAAYGPFILATLFGWAFATWGNPNIIFYGLSAFFALNVAMNWVMYARRGA
                     ATPC"
     gene            complement(7353..8720)
                     /locus_tag="PK812_05845"
     CDS             complement(7353..8720)
                     /locus_tag="PK812_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HRJ69113.1"
                     /translation="MSIAVDKGQQSRALWLSTIAFTVCFAAWTIFSIIGVQIKKDLNL
                     TDTQFGLLVGTPILTGSLIRLMLGIWTDQYGGRIVYTLVMLSAAAATWMLTYAYDYTT
                     FLIAALGVGIAGGSFAVGIAYVSKWFPKEKQGTALGIFGAGNVGAAVTKFLAPVVMVA
                     FGWKVVAQLWAVGLVVMAIVFWFTTADDPDLAARKKSGAKPEPISAMLEPLKNMQVWR
                     FSLYYFFVFGGFVALALWLPRYYVGVYGLDIVTAGLLGAAYSIPGSIFRILGGTLSDK
                     YGARKVMYWTFIGSVICTFFLSYPATKYVVNGIKGPMEFTIAIGFVPFTMLTFALGFF
                     MSLGKAAVYKHIPVYYPGRVGPVGGVVGLIGGLGGFILPILFGMANDYIGVWTSCFML
                     LFLIVAVSLIWMHAAIIAMEKQKHPELAGKRDLPELEALKIPAAANDPSTAAKILGVA
                     PAKKS"
     gene            8966..9664
                     /locus_tag="PK812_05850"
     CDS             8966..9664
                     /locus_tag="PK812_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018064458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRJ69114.1"
                     /translation="MRREDREEVARLPLFAGLGEKSLDRLLNGAFLQRFPAHVELIRE
                     GDPPDFLHMVVEGQVELFSRYRDRETTVSVIVPYDTFILAAVVLDHRYLKSARALAPC
                     RILMLPAEAVRQAFDTDPVFARGIAQELAMAYRGLVQELKNQKLRTSLERLANWLVRR
                     DQEAGGTGRFTLPFDKKVLAARLGMVPEVLSRTFATLQAYGVTVDGRSITLSDREALM
                     NLARPSPLIDDPEV"
     gene            9788..10744
                     /locus_tag="PK812_05855"
     CDS             9788..10744
                     /locus_tag="PK812_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRJ69115.1"
                     /translation="MLESLCALALAAKILPFCAPPPQRFAGYVEGEYVHLGPIEVAQI
                     RSITVRRGERVAPGQAVAVVETSDAELAVADAAARLAQVEAELANLRRGRRPEEIAVI
                     EASLASAEAQAREAKRALERRQDLFRRGFSPQAELDQAQTTLEVSDGKVRELKANLDV
                     ARLPARPNEILAFENRVAQARAAHGTAQWRLGQRTIRPLAAGRVTDIIRRTGEVAGPT
                     TPVLAMLPDGAVKLKVYVPQRVLSAITVGGTMIVRCDGCGTGMKATVSYVAPEPEFTP
                     PVIYSVETRQKLVYLVEARPEGAAMRLQPGQIVDVTLDGSAP"
     gene            10741..11655
                     /locus_tag="PK812_05860"
     CDS             10741..11655
                     /locus_tag="PK812_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008510446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ69116.1"
                     /translation="MTAAIDVVGLTKSFGKSKVVDDVALRVGRGEIVGFLGPNGSGKT
                     TTIRMICGLLTPDAGSGTVLGHDVRRDSRRIKREVGYMTQKFSFYEDLTIAENLIFVA
                     RLYGMTPVRAVVGATLDELGLTDRRDQLAGTLSGGWKQRLALAACIMHRPRLLLLDEP
                     TAGVDPKARREFWDEIHRLAARGLTVLVSTHYMDEAERCHRISYIARGRMLATGTVED
                     IVRDSRLHTYVVRGADAELTARIAALPEAAQVAPFGAVLHVVGKDANALGRALEPFGR
                     VQGVSVAPGQTSLEDVFIQFMTQAQGAP"
     gene            11712..12065
                     /locus_tag="PK812_05865"
                     /pseudo
     CDS             11712..12065
                     /locus_tag="PK812_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006326370.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
BASE COUNT         2101 a         3799 c         4011 g         2184 t
ORIGIN      
        1 ggccgaaccg cacttcctcc tcttcccagg cggcatcgag cgcctcgagg tcgtccggtt
       61 ccggatcggg ctcggcccgg agcgccgcga gcgcagcctc gtcgggcctg gctttcgcca
      121 gcgccagcac ggcgtccatc acggccgcgt aaggcgagga gcgcttgacc agcctctcct
      181 tcagggccgt gatcacgtgg gccggctggc cgacgagatc gatcgcctcc ttggccgggc
      241 gggtcgaggc atattcgagg aacagcggca gataatccgg cagttccgtg gtcacggggg
      301 aatagccatt ctcctcgtag accttgatca gatcgaccat cgcctggccg cggtcccggc
      361 tctcgccatg cacatgttcg aacaggtgca gggacagcga gcgggtgcgg tcgaacagca
      421 ggccgtaggc ctcctgccga tcgtagatgt caccatcggc aagatgatcc atcagccgat
      481 ggaccgccgc cgccacgcgc gccggcagga cgccttcacg gtcgatgacg aaccgcatgt
      541 cgccgatggc ctcgaccaga tccgggctcg gataggtgag aagcgcggag agaaccttga
      601 gcgtcttcat catgccatct ccatcggatt cttggccttc ggctttccgc cgaacaggtt
      661 cgtcggcgtc tcgccgtcgg agcagccgtt gccgaaggaa aagccgcagg agccgcgcag
      721 cccgtaggca tcctccgcca cctcgcgccg cgtcgtcgga atgacgaagc ggtcctcgta
      781 gttggcgatg gccatgatgt ggtacatttc ctcgatctgc aggcccgtga gcccgacctt
      841 cctggcgatg ccctcatcga tgacgccatc cacggtcttg gcgcgcatgt aggcgcgcat
      901 ggccagcatg cgctccagac ccttggcgac cggctcctcg tctcctgccg tcaggaggtt
      961 ggcgagatag cgcagcggga tgcgcaggtt gcgcacgtcc ggcatgtcgc catcgacacc
     1021 gatcttgccg gcttccgccg catggctgat cggcgacagc ggcgggatgt accagaccat
     1081 cggcagcgtg cggtattccg gatggagcgg gaaggcgacc ttccactcca tcgccatctt
     1141 ccagatcggc gactgctttg ccgattcgag ccaggcctcc ggaatgccct gcttgcgggc
     1201 ttccgcgatc accgccggat cgttgggatc gaggaacacc ttgagctgcg agtcatagag
     1261 attgcgctcg ctcttctcgc tggcagcagc ctcgatcttg tccgcgtcgt agagaatgac
     1321 gccgagatag cggatgcggc cgacgcaggt ctccgagcag accgtgggct ggccggattc
     1381 caggcgcgga tagcacagga tgcacttctc ggacttgccc gacgaccagt tgaagtagat
     1441 cttcttgtac gggcatcccg agacgcacat gcgccagccg cggcacttgt cctgatcgat
     1501 cagcaccaca ccgtcttcgg cccgcttgta gatcgcgccg gacgggcagg acgcgacgca
     1561 ggccgggttg aggcagtgct cgcacagcct cggcaggtac atcatgaagg tgttctcgaa
     1621 ctggccgtag atctccttct gcacgccttc gaagttggca tccttcgaac gcttcgagaa
     1681 ctcgccgccg agaatttcct cccagttcgg accccagtgg atcttctcca tccgctcgcc
     1741 ggtgatcagc gagcgcgggc gggccgtcgg gaaggcctcg agttcgggcg ccttctgcag
     1801 gtgctcgtag tcgaaggtga agggctcgta gtagtcgtcg atctccggca ggttcgggtt
     1861 ggcgaagatc tggctgagga tgcgccattt gccgccgatc ttcggctcga tcttgccgct
     1921 cgcggtacgc ttccagccac ctttccagcg ggtctggttc tcccattcct tgggatagcc
     1981 gatgcccggc ttggtctcga cgttgttgaa ccaggcgtat tccatgcctt cccggttcgt
     2041 ccaaacgttc ttgcacgtca ccgagcaggt gtgacagccg atgcacttgt cgaggttcag
     2101 caccatcgca atctgagcgc ggattttcat cgcgttatct ccttttcact cggcggcctg
     2161 gatggtcggt tcggcgtctt tcgccggccg ttccagccag tccacgttgc gcaccttgcg
     2221 gaccacgacg aactcgtcgc ggttggtgcc gatggtgccg tagtagttga agccgtagga
     2281 ttgctgggcg tagcccccga tcatgtgcgt cggcttgggg atcgcacggg tgaccgagtt
     2341 gtggatgccg ccccgctggc cggtcatctc ggagcccggc acgttcacga tcttctcttg
     2401 cgcgtggtac atgaagagcg ttccctcctt catgcgttgc gagaccaccg cgcgggcgac
     2461 gagcgctccg ttggcgttgt aggcctccac ccagtcgtta tcgacaatgc ccgccttcgc
     2521 cgcatccttc tcgctcagcc agacgatcgg tccgccgcgt gagagggtga gcatcatcag
     2581 gttgtcggtg taagtggagt ggatgcccca tttctggtgc ggcgtgatga aattgagcag
     2641 gatttccttc tcgccgttgg gcttgaggtc tttcaccggc gcgatggtct tcaggtccac
     2701 aggcggacgg tagacgcaaa gcgcctcgcc gaaggcccgc atccacaggt gatcctggta
     2761 gagctgctga cggcccgtca gcgtgcgcca ggggatcagt tcgtgaacgt tggtgtagcc
     2821 ggcgttgtag cagaccttct cgctctcgag gccagaccag accggcgagg agatgatctt
     2881 gcgcggctgg gcgacaacgt cgcggaagcg gatcttctcg tcctccttgg ggatggcgag
     2941 gtgggtgtgg tcgcgaccgg tggtcttcga cagcgctttc caggccttga ccgcgacttc
     3001 gccgttggtc tccggcgcga gcgacagaat gacttcgctg gcgtcgatat cagtctcgat
     3061 gcgggcaagc cccttggtcg gcccgtcctc agtgacgaca ccgttgaggg ccttcaggaa
     3121 ctcgacctca tgggcggtgt tccaggccat gcccttgccg ccgttgccga tcttctccat
     3181 gagcgggccg agcgaggtga agcgcttgta ggtgttggga tagtctcgct ccacgacggc
     3241 gaccgtcggc atggtcttgc cggggatcgg ctcgcactcg cccttcttcc agtccttgac
     3301 gtcaagggcc tgggcgatct cgttcgggct gtcgtgcatg atcgggttca gcacgacatc
     3361 cttctcgacg ccgagcactt ccggcgcgac gtcggagaac ttcttcgcga tggacttgta
     3421 gatgtcccag tcgctgcggg actgccaggc cggatccacc gcattggtca gcggatggat
     3481 gaacgggtgc atgtccgagg tgttgagatc gttcttctca taccaggtcg cggtcggcag
     3541 aacgatgtcc gagtagacgc aggtcgtgga catgcggaag tcgagcgtga ccaggaggtc
     3601 gagctttcct tccggcgcct tgtcgtgcca gaccgcttcc agcggcttgg catggccctg
     3661 ctcgccgagg tccttgccca tcacgccgtg cgaggtgccg agcaggtgct tgaggaagta
     3721 ctcatgcccc ttgccggacg agccgagcag gttggagcgc catacgaaca ggttgcgcgg
     3781 ccagttcttc ggattgtccg ggtcctcgca ggacatcttc agttcgccgg acttcagctt
     3841 ggcggcgacg tagtccttcg cctccatgcc ggccgccgcg gcgtccttcg agacctgcag
     3901 cggattggtc tggagctgcg gcgcggaggg cagccagccc atgcgctcgg agcggatgtt
     3961 gtagtcgatg atcgcgccgt cccaggggcc ggccggcgcg gtcggcgaga cgatctcgcc
     4021 gatcttgacc tgctcgtagc gccactggtc cgtgtgggcg taccagcagg aggttgagtt
     4081 ctgctggcgg ggcgggcggc cccagtcgag gccgaaggcc agcggcagcc atcccgattg
     4141 cgggcgcagc ttctcctggc cgacatagtg cgaccagccc ccgccggact ggccaacgca
     4201 tccgcacatc accaacatgt tgatgacgcc gcggtagttc atatcggcat ggtaccagtg
     4261 gttcatcgcc gcgccgatga tgaccatcga gcgtcccttg gtcttctcgg cgttgagagc
     4321 gaactcgcgg gcgacagtga tgatcttgtc gcgcgtgacg ccggtgatct tctcagccca
     4381 ggccggggtg tagggcacat cctcgtcgta ggatttcgcc acgttggcgc cgccaaagcc
     4441 gcgatcgacg ccgtagttgg cgaggaacag gtcatagacc gacgcgacga gaacatcgcc
     4501 gtccgtcgtc ttgaccttct tgaccggcac cttgcggttg agcacgctgg cgtgactgtc
     4561 ggagacgaag tgctcgttct tgatgttgcc gaaatacggg aaggcgacat cctcaatgcc
     4621 gtccttgacc tcggcgagcg acagacggag cgtcgtggca tcgccgttgc tcgccttctc
     4681 ctccaggttc cacttgcccg tttcgcccca gcgatagccg atggagccgg taggcgcgac
     4741 gagcttgccg gacttctcgt cgaaggccag cgtcttccat tccggattgt tggtctcgcc
     4801 gagcgcgccc ttgatgtcgg aggcgcgcag gaaacgatcc ggcaccagct ttccatcctg
     4861 ctgcaccagc ttcacaagca tgggcatgtc ggtgtactgg cggacgtagt cgcggaagta
     4921 ctgggcctga cggtcgacat ggaactcccg caggatgacg tggcccatgg ccatggccag
     4981 cgccgcatcc gtgccctgct tcaccgacag ccagatgtcg ccgaacttgg aggcttccga
     5041 gtagtccggg cagatgaccg cactcttggt gccgcgatag cgcgcctcgg tgtagaagtg
     5101 cgcatccggc gtgcgggtct gcgggacgtt cgagccccac aggatgatga agccggcgtt
     5161 gtaccagtcg gccgattccg gcacgtccgt ctgttcgccc caggtctgcg gcgaagacgg
     5221 cggcaggtcg cagtaccaat cgtagaacga catgcagacg ccgccgatca gcgacaggta
     5281 gcgcgatccg gccgcgtagg agaccatcga catcgccggg atcggcgaga agccgaggat
     5341 gcggtcaggg ccgtatgtct tcgcggtgta ggcgttggcc gaggcgatga tctcggtgac
     5401 ctcgtcccag ctcgcccgca cgaacccgcc gtgaccacgc ttcttggtat aggatgcgcg
     5461 tttgcccggg tcctcgacga tcgacttcca ggcagcgacc ggcgtcaggg tggcgcgcgc
     5521 cgcacgccac agcttcagca gacgcgagcg caccagcggg tatttcacgc ggttgcccga
     5581 gtagagatac cagctgtagc tcgcaccgcg cgaacagccg cgcggctcgt ggttcggcag
     5641 atcgggccgc gtgcgcggat agtccgtctg ctgggtctcc caggtgacga tgccgccctt
     5701 gacatagatc ttccacgaac aggagccggt gcagttcgcg ccgtgggtgg agcgcacgac
     5761 cttgtcgtgc tgccagcgct tgcggtagcc ctcctcccag gcgcgatcct cggaggtcgc
     5821 aacgccgtgg ccgttcgaga agggctctct gagcttcgag aagaaggtga gccggtcgag
     5881 aaagtgagac atgatagttt cccgttttcc tctggattag caaggggttg cagcgccgcg
     5941 ccgggcgtac atcacccagt tcatggcgac gttgagagcg aagaaggccg acagcccgta
     6001 gaagatgatg ttcgggttgc cccaggtggc gaaggcccag ccgaacagcg tggccaggat
     6061 gaagggaccg taggccgcga tcgcgccggt gaagccgatc acgccgccgg cctgacgcgg
     6121 cgggaagatc atcggcatct gcttgaaggt cgaggcgttg ccgatgccgg cgaagaagaa
     6181 caggccaagc atcgcgccga caaagtacgg gaaggaggcc atcgaggttg gctgggtgta
     6241 catcggcacg accgccgcgc agatcagcat gccgaggccg gcccaatggg tcacgcgggc
     6301 gccgccgagc ttgtccgaga tcgggccggc gatgatgcgc gagatggagc cgacgagcgg
     6361 gccatagaag gcgaatgcca gcggatccgg cgcgccaggc aaggccccgt agcattgctt
     6421 gatcagcagc gggaacgtgc cggacaggcc cgagaacgcg ccgaaggtca tgatgtacag
     6481 ggacgtcatc gaccaggtgt gacgcgagcg gaagatatcg aactgctccg cgaagttcgc
     6541 cttgatcggc accgacttca gcagcagcca ggccgaaatg ccgaagaccg cgatgaaggg
     6601 aatgtagatc gccgtcgcgt tctgcagcca gatcgacttc tgcgcgccgg cgaccgtcat
     6661 ggtctggttg ccgcccatcc aggtcgcgcc gcccatcagc gccacggtga tgatccaggg
     6721 cgtgatgaac tgcacgacgg agacgccgaa gttgccgatg ccggcctgga tcgccagcgc
     6781 ggtaccctgc aggcgcttgg ggaagaacat ggatgtcgac ggcatgaagg aggagaagtt
     6841 gccgccgccg aggcccgcca ggaacgccag cagcatcagc atccagtagg gcgtcgtcgg
     6901 atcctgcacc gcgtagaacc agccgacggc agggatcagc agcgacagcg tcgagaggct
     6961 gaccaccgcc cgcgtgccgt acatcggcgt caggaacata tggatgagcc gcagcgtgcc
     7021 gccggccagt cccggcatcg ccgcgagcca gaacaactga ctggtggtca gcttgaagcc
     7081 gatctgcggc aggcgcacga ccagcgccga caccacgaac cagacgatga aggccatgat
     7141 caggttggcg gtggtgatcc agagcgtcag ccacgcccgg ctggaaccgc cttccttcca
     7201 gaaggtctcg ttctcgggtt cccagcgtgt cagccaggat gcgccgcgcg ccgtcgaggc
     7261 acttgtgtct gcgttggcca tcatggcctc cttgagggtg aaaggctgag ccgggccgcg
     7321 cggcgggacg ccgcgcggtc aagggtggtt cgtcaggatt tcttggccgg agcgacgccc
     7381 aggatcttcg ccgccgtgct tgggtcgttg gcggccgccg ggatcttgag ggcttccagt
     7441 tcgggaaggt cgcgcttgcc ggcgagttcg gggtgcttct gcttttccat cgcgatgatg
     7501 gcggcgtgca tccagatcag gctgaccgca acgatcagga agagcagcat gaagcagctg
     7561 gtccacacgc cgatgtagtc gttggccatg ccgaacagga tcggcaggat gaagcccccg
     7621 aggccgccga tgaggcccac gacgccaccc accggaccca cgcgaccggg gtagtagacc
     7681 gggatgtgct tatagaccgc cgccttgccc aggctcatga agaagcccag cgcgaaggtg
     7741 agcatggtga aggggacgaa cccgatggcg atggtgaatt ccatcggtcc cttgatgccg
     7801 ttcaccacat atttcgtggc gggatagctc aggaagaagg tgcagatgac cgagccgatg
     7861 aaggtccagt acatgacctt gcgggcgccg tacttgtccg agagcgtgcc accgaggatg
     7921 cggaagatcg aacccgggat cgagtaggcc gccccgagca ggcccgcggt cacgatgtcg
     7981 aggccgtaga cgccgacata gtagcgcggc agccaaaggg cgagggcgac gaaaccgccg
     8041 aagacgaaga agtaatagag cgagaagcgc cagacctgca tgttcttgag cggttccagc
     8101 atggccgaga tcggctcggg cttggcgccg gacttcttgc gcgcggcaag gtcgggatca
     8161 tcggctgtcg tgaaccagaa cacgatcgcc atgacgacca gtccgacggc ccagagctgc
     8221 gccaccacct tccagccgaa ggccaccatg accacagggg cgaggaactt cgtcaccgcc
     8281 gcgccgacat tgcccgcgcc gaagatgccg agcgcggtgc cctgcttttc cttcgggaac
     8341 cacttcgaga cataggcgat gccgacggca aacgatccgc cggcgatgcc gacgcccagc
     8401 gccgcgatca ggaacgtggt gtagtcgtag gcgtaggtga gcatccaggt cgctgcagcg
     8461 gccgacagca tcaccagcgt gtagacgatg cgtccgccgt actgatcggt ccagatgccg
     8521 agcatcagac ggatcaagga ccccgtgagg atcggcgtgc cgaccagcag gccgaactgc
     8581 gtgtcggtca ggttcagatc ctttttgatc tgaacgccga tgatcgagaa gatcgtccag
     8641 gccgcaaaac atacggtgaa ggctatggtc gagagccaga gcgccctgct ctgctggccc
     8701 ttgtcgacgg cgatgctcat ggtgctcccc tgaggctgtt ttcgctttgc gcgtgccaga
     8761 ggggagcggg aaggcgttct ttcctttgat tcaggtcaac ggactcgacc caccccgata
     8821 ttttcaagac acgaggaaaa aaccatcaag atgcccttcg cacggacgtc aaacgggcaa
     8881 tctcagccag gcaattttgc gcttgaccga aatcaaatac tcgactttcg gatagtgtca
     8941 gagaagcagc gtcggggagg gactcatgcg ccgcgaagat cgcgaggaag tagcgcgtct
     9001 gccgctgttt gccgggcttg gagaaaagag tctcgaccgc ctgctgaacg gcgctttcct
     9061 acaacggttt cccgcccacg tcgaactcat ccgggaaggc gatccgcccg acttcctgca
     9121 catggtcgtg gaggggcagg tcgagctgtt ttcccgttat cgtgatcgcg agacgacggt
     9181 ttcggtcatc gtgccttatg acacgttcat cctggcggcg gtcgtccttg atcaccgcta
     9241 tctcaaatcc gccagggcac tggccccctg ccggatcctg atgctgccgg ccgaggccgt
     9301 gcgccaggcc ttcgacaccg atcccgtgtt tgcccgaggc atcgcccagg aactggccat
     9361 ggcctatcgc ggcctggttc aggaactgaa gaaccagaag ctgcggacca gtctagaacg
     9421 gcttgccaac tggctcgtgc ggcgcgacca ggaggccggc ggaaccggcc gcttcaccct
     9481 gcccttcgac aagaaggtgc tcgcagcgcg ccttggcatg gtgccggaag ttctctcgcg
     9541 caccttcgcc accctgcagg cctacggcgt gaccgtggac gggcgttcga tcacgttgtc
     9601 ggaccgtgag gcgctgatga acctggcgcg cccatcgccg ctgatcgacg atccggaggt
     9661 gtgagagcga aggctggtgc gctggcccgc gtcgcgcggg ccggacatcg ccttggaagc
     9721 ggcgcgaggc gctggtcttg gctttcgttg aagcctatcg tcagccacgt caccggagaa
     9781 ccggccgatg cttgaatccc tgtgcgccct ggcccttgcg gcgaagatcc tgccgttctg
     9841 cgctccgcct ccgcagcgct tcgcgggata tgtcgaagga gaatacgtcc atctcggtcc
     9901 gatcgaggtg gcgcagattc gttccatcac ggtgcgccgg ggggagcgcg tggcgcccgg
     9961 ccaggcggtt gccgtggtcg aaaccagcga cgcagaactc gcggtcgccg atgccgccgc
    10021 acggctggcg caggtcgaag cggaactggc caatctgcgt cgcggtcgcc ggccggagga
    10081 aatcgcggtg atcgaagctt cgctcgcctc cgctgaagcg caggcgcgcg aggcgaagcg
    10141 cgcgctcgaa cgccggcagg acctgttccg acgtggattc tcgccgcagg ccgagcttga
    10201 ccaggcacag accacgcttg aggtcagcga cggcaaggtg cgggagctga aggccaacct
    10261 cgacgtggcc cggctgccgg cgcggccgaa cgagattctc gccttcgaga accgggtcgc
    10321 gcaggcccgt gcggcgcacg gcacggcgca atggcggttg ggacagcgca cgatccgacc
    10381 gctggccgcg ggccgcgtca cggacatcat ccggcggact ggcgaggtag ccggccccac
    10441 gacgccggtt ctggcgatgc tcccggatgg cgcggtgaaa ctgaaggtct atgtgccgca
    10501 acgcgttctc tccgcgatca ccgtcggcgg cacgatgatc gtgcgttgcg atggctgcgg
    10561 gaccggcatg aaagcaaccg tcagttatgt cgcgccggag ccagagttca cgccgccggt
    10621 gatctactcc gtcgagacac gccagaaact ggtctacctt gtcgaggcaa ggccggaggg
    10681 ggcggcgatg cgactccagc cggggcagat cgtcgatgtc acgctcgacg ggagcgcgcc
    10741 gtgacggcgg ccatcgatgt cgtcggcctg accaaaagct tcggcaagtc caaggtcgtc
    10801 gacgacgttg ccctgcgggt cggcaggggg gaaatcgttg gctttctcgg acccaatggt
    10861 tctggaaaga cgacgaccat ccgcatgatc tgcggcctgc tgacgccgga cgccggctcg
    10921 ggcaccgtgc ttggccatga cgttcgccga gacagccggc gcatcaagcg cgaagtcggc
    10981 tacatgacgc agaagttctc cttctacgag gacctgacga tcgccgagaa cctgattttc
    11041 gtcgcgcgcc tctacggcat gacaccggtg cgcgccgtgg tcggcgccac gctcgacgag
    11101 ctcggcctga cggaccggcg cgaccagctt gccggcacgc tttccggcgg ctggaagcag
    11161 aggctggcgc tggccgcctg catcatgcac cgccccaggc tgctgctgct cgacgagccg
    11221 actgccgggg tcgatcccaa ggcgagacgc gagttctggg atgagatcca ccggctcgcc
    11281 gcgcgcggcc tgaccgtgct tgtctccacc cactacatgg acgaggccga gcgctgccat
    11341 cgcatcagct acatcgccag gggccgcatg ctggcgaccg gcaccgtcga ggacatcgtt
    11401 agggattcgc ggcttcacac ctatgtcgtg cgcggcgcgg atgccgaact gacggcgcgt
    11461 atcgccgcct tgcccgaggc cgcgcaggtc gcgccgtttg gcgcggtgct gcatgtggtg
    11521 ggcaaggatg ccaatgcgct cggccgggcg ctcgaaccgt ttggacgggt tcagggcgtg
    11581 agcgtcgcac ccggccagac gagccttgag gacgtgttca tccagttcat gacgcaagcg
    11641 cagggggcgc cgtgaacagc cccgtgtccc tgcgccgcct tgccgcgatc ctggcgaagg
    11701 aattcgtcca gatgcgtcgc gacagggtca ccttcgcgat gatgctgctg gtgcccttga
    11761 tccagctcgt gctgttcgga ttcgccatca acaacgatcc gaaaggactg ccgagcgcct
    11821 tgctcgcact tggccaggac cgttatgccc gcgccatcgt cgtcgcgctg gaaacaagcg
    11881 gctactaccg cttcagccat cgccctgaga gcgcggcgga agcggagtcc ctgatcgcgg
    11941 cggggcgcgt ttccttcgtc gtcaccattc cggtggactt tggcgtgcgg gtcgagcgcg
    12001 gggacaagcc tcacatcctg attgaagccg atgccaccga tccggcggcg gccagcggcg
    12061 ccatctgatg acgccatgaa taccaagact ccgga
//
LOCUS       DAOMXN010000052        11811 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_2933_length_11811_cov_11.513610, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000052 DAOMXN010000000
VERSION     DAOMXN010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 11811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11811
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2933_length_11811_cov_11.513610"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1165)
                     /locus_tag="PK812_05870"
     CDS             complement(<1..1165)
                     /locus_tag="PK812_05870"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase family protein"
                     /protein_id="HRJ69117.1"
                     /translation="MALSHLLKAGAAALVLSFLVGCEEDARLSRNGRHYVPIPGHMIS
                     LMAEKGTTKHQPILIRAFKKESELEVWKQNLTGEYVHLKTYPMCRWSGQLGPKVREGD
                     RQVPEGFYSITPAQMNPNSSFYLSFNVGYPNAYDRAHDRTGSLIMVHGACSSMGCFSM
                     TDEQIAEIYAITREAFAGGQKAVQLQSFPFRFTPQNMAKFRADPNMPFWKTLKEGSDS
                     FEVSKRETQVSVCERRYVFNRTAKDNARFEAAAACPPSETNDAVASAVAAKARNDETK
                     VAELVQSGTRAVRRIYKDGDQHPSFKTVQMAKVSSDGQYLEPTKANVSRVAEVSRPDD
                     LEHGPIEIAIEDLKGKASLPARQLAVSLPAKKDALTTGAVATAASATSAPQPKS"
     gene            complement(1306..2259)
                     /locus_tag="PK812_05875"
     CDS             complement(1306..2259)
                     /locus_tag="PK812_05875"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HRJ69118.1"
                     /translation="MRAYLDFEKPVAEFEKKLAELRALAEGGEHAGVTEDVARVEAKA
                     REALKEIYANLTPWQKTQVARHPERPHCVDYIKALITDFTPMAGDRCFAEDEAVVGGF
                     GRFHGASICVIGQEKGSDTDSRIRHNFGMARPEGYRKAVRLMETADRFAIPVLCLVDT
                     PGAYPGIDAEERGQAEAIARSTDACLALGVPNVSVIIGEGGSGGAVAIATANRVAMLE
                     HAIYSVISPEGAASILWRDSARAQDAASTMKITAQDLLKFGVIDTIIPEPVGGAHRDA
                     EATFQAADRAIFNAFTELSGRNAEEIRTARAEKFLAIGRRI"
     gene            complement(2404..3333)
                     /locus_tag="PK812_05880"
     CDS             complement(2404..3333)
                     /locus_tag="PK812_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HRJ69119.1"
                     /translation="MSSRGHLRAFLDMMAAERGASRNTLLAYERDLDDYIAFIAGQGV
                     DPLDVATGQVRDYLGELERRGAKPATAARKLSAIRQFHGFLYREGHRGDDPTAVLEGP
                     RRRVRLPKVMSVAEVDRLLSQAAAGIDEGARPASQRLAAARLSALLELLYATGLRVSE
                     LVSLQRTAMPAGKDTMVIRGKGNKERLVVITEAARNAVARYTALRDMLMPQERSRFLF
                     PADGESGHLSRQVFARDLKAAAARAQIAPDRVSPHVLRHAFASHLLQNGADLRVIQQL
                     LGHADIATTQIYTHVLDARAASMVRDLHPLQDE"
     gene            complement(3341..3484)
                     /locus_tag="PK812_05885"
     CDS             complement(3341..3484)
                     /locus_tag="PK812_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008598908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69120.1"
                     /translation="MLPIVRLIVIVAVLAVVGYASMMALTQFVEPEPRDYSVTVPASR
                     FNK"
     gene            3625..4212
                     /locus_tag="PK812_05890"
     CDS             3625..4212
                     /locus_tag="PK812_05890"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HRJ69121.1"
                     /translation="MDRPATARRGQVESAIVDALGERSIVLVGLMGAGKSSLGKRLAA
                     RLAIPFVDADTAIEQAAGMTIPEIFDRHGEADFRAGERRVIARLLGEGPQVLATGGGA
                     FMSEETRERIRAFGISVWLKADLDILMRRVRKRGDRPLLRTDDPEGTMRRLMDTRHPV
                     YAEADITVVSGEVPHEVMVESLVSALAGHLLAETH"
     gene            4225..5358
                     /gene="aroB"
                     /locus_tag="PK812_05895"
     CDS             4225..5358
                     /gene="aroB"
                     /locus_tag="PK812_05895"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008598911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HRJ69122.1"
                     /translation="MSNSAQESRVIVPVALAGRSYDIVIGRGLVAEAGARIAALAPGA
                     RAAIVTDETVAGLHLAALRTALARAGIAATDIVLPAGEATKNWQTLGDVVDRIIGARL
                     ERGDVVIALGGGVIGDLTGFAAAIVRRGMRYVQIPTTLLAQVDSSVGGKTAVNSRHGK
                     NLVGAFHQPALVLADTAVLDTLGPREFRAGYAEVAKYGLIDRPDFFEWLESGAWSGIF
                     SGGPERERAVEICCAAKAAVVARDETETGDRALLNLGHTFGHAIEAVTGYDARRLVHG
                     EGVAIGMALAHRFSQRLGLCPGQDAFRVSRHLEAVGLPTRLAEVPGGIGSAEALLAAI
                     RQDKKVSRGTLTFILTNGLGRSFIARNVAETEVADFLAEELAG"
     gene            complement(5390..7189)
                     /locus_tag="PK812_05900"
     CDS             complement(5390..7189)
                     /locus_tag="PK812_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019903355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015441 - ABC-type beta-glucan transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015775 - beta-glucan transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan ABC transporter ATP-binding protein/
                     permease"
                     /protein_id="HRJ69123.1"
                     /translation="MSLFRLYARVVGQLGAELKIGVLLLFANLALSIAQFAEPYLFGR
                     IINVLTGAQGQAKPTFETLTPLIMAWLFFGLFTIGGGVLVALHADRLSHRRRLGVMAG
                     FFEHVLALPLSFHTQTHSGRVLKVMLEGSNGMAWLWLGFFRDHCAALLSLFVLLPFTL
                     FINWRLGLLLVVLVVVFAALTAVVLRKTESKQGAVERYHTDLAEHASDALGNIPVIQS
                     FTRVQAELHSLRDIIDRLLAAQIPVLSWWAMASVATRAASTLTVTSIFLLGTWLHLNG
                     LASIGDIVTFMGYATLLIGKLEAAVGFVNSLFMQAPKMEEFFEVLDTTPAVHDKPGAI
                     DAGRMDGAVAFDTVSFSYDGKRSAVADVSFDIPKGQTVALVGATGSGKSTTLGLLHRA
                     FDPQSGRILIDGRDIRDVSLTSLRRNIAVVFQEPMLFARSIRENLQVGRPDASDEEMM
                     SALERAQALDFIKRQPDGLDTVIGERGRSLSGGERQRLSIARALLKNPPLLILDEATS
                     ALDAATEQKLQAALDAVMRGRTTFVIAHRLATIRNADRILVFEQGRIVESGTFETLVA
                     QGGRFATLAKAQFMAGTEGVARPPDAKGEVFAS"
     gene            complement(7271..9712)
                     /locus_tag="PK812_05905"
     CDS             complement(7271..9712)
                     /locus_tag="PK812_05905"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRJ69124.1"
                     /translation="MKLLRVFQFLAVALMAFFLFAAVYTSSLIGERQQTIQKSARYNI
                     AWLAAQASVEVARLVQVVNAFGVPKSGVDQEEVQLRLDIVQSRMKLIKGGEFNDFVSQ
                     DRERQEIVEELTQTIEAAQPLIDALDKQPENVLRLMRMLTPLDKRLSRLAAAANVFGG
                     DRVDEDQHALLRLHWTFSILMLGIIACGMALMAMMFWNFKVLKRTNGELKELAANLEA
                     TKIDLETANQGTQTANAELNLHNQILKLRDAELRTQNLRFDAALNNMSHGLCMVGADS
                     KLIVCNQQFRELFYLDAETVRPGTALNALLVRIGIALKLGDEATLELLREQEALVSNR
                     SASSFTREYADGRIFAIYHQPMVDGGWVATYEDITERKRTEEQIAHMAHHDALTGLPN
                     RAFFLKHLEDALTKARRGDAGCAVLCLDLDRFKAVNDTLGHPVGDLLLKAVSARLKAA
                     VDKGDVVARFGGDEFAILKNDLSDPTVLHVLGARLVKDISTPFQLDGNEVSVGTSVGI
                     AIGFQDGASSEQLLKNADLALYHSKEEGRGAYHFFEARMDAKLQARRLVEVDLKRAIP
                     NNELELHFQPLVTLSNTRISGFEALIRWRHSERGMISPGEFIPIAEEVGLIVPIGEWV
                     LRRACAEALNWPQGIKIAVNLSAVQFRDRNLVGTVRSALEQTGLEPGRLELEITETAL
                     VQDMDFTVEVLHQLRALGVRISLDDFGTGYSSLSYLRSFPFDKIKIDQSFVRELGNRP
                     DCLAIVRSVASLGHMLGMTTTAEGVETEEVLNMIREMGCSEVQGYFYSRPVRRDDLGA
                     LIQKFNGTVPATTAA"
     gene            complement(9786..10253)
                     /locus_tag="PK812_05910"
     CDS             complement(9786..10253)
                     /locus_tag="PK812_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003500270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRJ69125.1"
                     /translation="MGLSLPASAGDLKAPQEKIVLTVNGKIGVTNVDDTAQFDRGMLE
                     KLGMTTIETTTPWYSGTMKFEGIPMESLMKAIGAKGDRLVAVALNDYSTEIPIEDFAK
                     YKPILALKRNGEYMPVKDKGPLFIVYPYDSDPELKSQKFYSRSAWQVARLTVK"
     gene            10617..11282
                     /locus_tag="PK812_05915"
     CDS             10617..11282
                     /locus_tag="PK812_05915"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF013398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HRJ69126.1"
                     /translation="MKSNVLRLNAARLCNETVEGFTTASERNALKVAAAQKIEELFDI
                     LKIDYRNDHNTRETPQRVAKMLVEETLSGRYNEPPKITDFENAQNYDELIVTGPIEVR
                     STCAHHMMPIYGSAYIGVLPATDGKIIGLSKYDRIVDYFSSRLQIQEELVKQIEKFIV
                     EKTQPRGLAVRISAVHMCKTQRGVRASHQSRMVNSTFYGEMSRNQSLRAEFLQECAVL
                     DAR"
     gene            complement(11331..11777)
                     /locus_tag="PK812_05920"
     CDS             complement(11331..11777)
                     /locus_tag="PK812_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HRJ69127.1"
                     /translation="MMGSLGSWGWLALGLLLIALEVLVIPGGFLLWIGIAALVMGAIV
                     ALVAISWQLELVVFGLLALAASFLAWKLHHGRDRATDAAETLHDRSSQLIGRRFALEE
                     AIVDGFGRVRIDDTLWRVSGPALDKGAQVRVTGVDGATLVVSAAEV"
BASE COUNT         2051 a         3862 c         3841 g         2057 t
ORIGIN      
        1 acgacttcgg ctgcggcgcc gaagtggcgg aggcggcggt cgccacggct ccggtggtga
       61 gcgcatcctt cttcgccggc aggctgacgg caagctggcg ggccggcagg ctggccttgc
      121 ccttcaggtc ttcgatcgca atctcgatcg ggccatgctc cagatcgtct gggcggctga
      181 cttccgcgac gcgcgaaaca ttggccttgg tcggctcgag gtactgcccg tcgctggaaa
      241 ccttcgccat ctgcaccgtc ttgaacgagg gatgctggtc gccatccttg tagatgcgac
      301 gcacggctcg ggtgccggac tggacgagtt cagcgacctt ggtctcgtcg ttgcgcgcct
      361 tggccgcgac ggcggaggcc acggcatcgt tggtctcgga gggcggacag gcggcggcgg
      421 cttcgaaacg ggcgttgtcc ttggccgtcc ggttgaagac gtagcggcgc tcgcagaccg
      481 aaacctgcgt ttcgcgcttc gaaacctcga aggaatcgga accttccttc aacgtcttcc
      541 agaacggcat gttgggatcg gcccggaact tggccatgtt ctgaggcgtg aaccggaagg
      601 ggaaggactg cagctgcacc gccttctggc caccggcgaa ggcctcgcgc gtgatggcgt
      661 agatctcggc gatctgttcg tccgtcatgg agaaacagcc catcgaggaa caggcgccat
      721 gcaccatgat gaggctgccg gtgcggtcgt gcgcgcggtc ataggcgttg ggatagccaa
      781 cgttgaagct gagatagaaa gacgaattcg ggttcatctg cgccggcgtg atcgagtaga
      841 agccttccgg cacctgccgg tcgccttcac gcaccttggg gcccagctgg cccgaccagc
      901 ggcacatcgg ataggtcttg aggtgaacgt actcgccggt caggttctgc ttccagacct
      961 ccagttcgct ctccttcttg aaggcgcgaa tgaggatcgg ctggtgcttg gtcgtcccct
     1021 tctccgccat gagggaaatc atgtggccgg gaatgggaac ataatggcgg ccgttgcgcg
     1081 acaggcgcgc gtcttcctcg cacccgacga ggaagctcag gaccagagcg gccgctccgg
     1141 ccttcagcaa atgtgacaat gccatgcgtt ccagttccga actcgaccgt cctgtcacag
     1201 gatcaggctg attagccttg cctgtaggtt actgcaatcc tgctaccgcg atcaattatc
     1261 tcaacacacc gttaacagat catggccgga cagcgttgcg gccggtcaaa tccgccggcc
     1321 gatggccagg aatttttccg cccgggccgt gcgaatttcc tccgcattcc ggcccgacag
     1381 ttccgtgaac gcattgaaga tcgcccggtc cgccgcctgg aacgtcgcct ccgcatcgcg
     1441 atgcgcgccc ccgacgggtt cggggataat cgtgtcaatt acgccgaatt tgaggagatc
     1501 ctgggcggtg atcttcatgg tggaggcggc gtcttgcgca cgggcgctgt cgcgccacag
     1561 gatcgaggcc gccccttccg gcgaaatcac gctgtagatc gcatgttcga gcatggcgac
     1621 gcggttggcg gtggcgatcg ccaccgcgcc gcccgaaccg ccttcgccaa tgatcaccga
     1681 gacgttcggc acgcccagcg ccaggcaggc atcggtggac cgggcgatcg cctcggcctg
     1741 cccgcgctcc tccgcgtcga ttccgggata ggcgccgggc gtatcgacca ggcacaggac
     1801 ggggatggcg aagcggtccg cggtctccat caggcgcacc gccttgcgat agccctccgg
     1861 gcgcgccatg ccgaaattgt ggcggatgcg cgagtccgta tccgatccct tttcctgccc
     1921 gatgacgcag atggaggcac cgtggaaccg gccgaagccg cccaccaccg cctcgtcctc
     1981 ggcgaaacag cggtcgccgg ccatcggcgt gaagtccgtg atcagcgcct tgatatagtc
     2041 gacgcaatgc ggccgctcgg gatggcgcgc cacctgcgtc ttctgccagg gcgtcagatt
     2101 ggcatagatt tccttgagcg cttcgcgcgc cttggcctcg acccgcgcca cgtcctccgt
     2161 gacgccagca tgctcgccgc cctcggcgag ggccctgagc tccgcgagct ttttttcgaa
     2221 ctcggcgacc ggcttttcga aatcgagata ggcgcgcatg gactcttctg caaagcgacg
     2281 gaaccaggga ccggaccgcc tgccggtccg gaccggaacg ctccgacccg aggccgcgtc
     2341 catacaggaa gcgcgccctc agggaaagtg cggcctatgg ccaaggctgg ctgagtcgcg
     2401 acgctactcg tcctggagcg gatgcaggtc acgcaccatg gatgcggcgc gcgcgtccag
     2461 cacatgggtg taaatctgcg tcgttgcgat gtcggcatgg cccagcaact gctggatgac
     2521 gcgcaagtcg gcgccgttct gcagcaggtg gctggcaaag gcgtgccgca ggacatgcgg
     2581 gctgacccgg tccggtgcga tttgcgcgcg ggcggcggcg gccttgagat cgcgcgcgaa
     2641 cacctggcgt gacagatggc cgctctcgcc gtccgccgga aacaggaacc ggctgcgctc
     2701 ttgcggcatc agcatgtcgc gcaacgccgt gtagcgcgcc acggcgttcc gtgccgcctc
     2761 ggtaatgacg acgagccgct ccttgttgcc cttgccgcga atgaccatcg tgtccttgcc
     2821 cgccggcatc gcggtccttt gcagcgaaac cagttcgctg acgcgcaatc ccgtggcgta
     2881 aagcaattcc aggagcgcgg acaggcgcgc ggcagccaga cgctgcgaag ccggccgggc
     2941 gccctcgtcg atgccggcgg ccgcttgcga cagcagccgg tcgacttccg cgacgctcat
     3001 caccttgggc agcctgaccc tgcgcctcgg tccttccagc acggcggtgg gatcgtcgcc
     3061 ccggtgtccc tcgcgataca ggaagccgtg gaactggcgg atcgccgaca gcttgcgcgc
     3121 tgcggtggcg ggtttggcgc cgcgccgttc gagctcgccc agatagtcac gcacctgacc
     3181 ggtcgcgacg tcgagcgggt ccacgccctg cccggcgatg aaggcaatgt agtcgtcgag
     3241 gtcgcgctcg taagccagca gcgtgttgcg actggcgccg cgctctgccg ccatcatgtc
     3301 gaggaaagcg cgcagatgtc cgcgcgagct catcggcagt tcacttgttg aaccgagacg
     3361 ccggcaccgt cacggaatag tcccgcggct cgggttcgac gaactgcgtc agcgccatca
     3421 tcgaggcata gccgacgaca gcgagaacgg cgacaatcac gatgaggcgc acgatcggca
     3481 acatgccaaa gctcccattc ccgtttgccc cgccgcatat ctggcgcatc gcggcttcgc
     3541 gggcaagcca tgaggctgat ttgcctcgcg ccggttccgt gatagcgagg ccgcgctccg
     3601 cccgcggagc ggcacggaac gggaatggac aggccggcga cagcaagacg gggacaggtc
     3661 gaatcggcga tcgtcgacgc actcggcgag cgctcgatcg tgctcgtcgg gctcatgggg
     3721 gccggaaaat cctcgctcgg caagcgtctt gccgcgcggc tcgccattcc cttcgtcgat
     3781 gccgacacgg ccatcgagca ggcggccggc atgaccattc ccgagatttt cgaccggcat
     3841 ggggaagccg acttccgtgc cggtgaacgg cgtgtgatcg cccgtctcct gggcgaggga
     3901 ccgcaggtcc tggcgaccgg cggcggcgcc ttcatgagcg aggaaacccg ggagcgcatc
     3961 cgcgccttcg gcatttcggt ctggctgaag gcggacctcg acatcctgat gcgccgcgtc
     4021 cgcaagcgcg gcgaccggcc gctgttgcgc accgacgacc ccgaaggcac gatgcgccgg
     4081 ctcatggata cgcgccatcc cgtttacgcc gaggccgaca tcaccgtggt ttccggcgag
     4141 gttccccacg aggtcatggt ggaatcgctg gtttcggcgc tggctggcca tctgctcgcc
     4201 gaaacgcact gaaccgaggc tcccgtgtcc aacagcgccc aagaatcccg cgtcatcgtc
     4261 ccggtcgccc tcgccgggcg cagctatgat atcgtcatcg ggcgcggtct tgtcgcggag
     4321 gcaggcgcgc gcatcgcggc gctggctccc ggggcgcgcg ccgccatcgt caccgacgaa
     4381 acggtcgcgg gcctgcatct cgccgcgctc aggaccgcgc ttgcgcgggc cggaatcgcg
     4441 gcgacggaca tcgttctgcc cgcaggggaa gccaccaaga actggcagac gctcggcgac
     4501 gtggtggatc ggatcatcgg ggcgcggctg gaacgcggcg atgtcgtcat cgcccttggc
     4561 ggcggggtga tcggcgacct caccgggttc gccgccgcca ttgtccggcg cggcatgcgc
     4621 tacgtgcaga tcccgaccac cctgctggcg caggtcgatt cctcggtcgg cgggaagact
     4681 gccgtcaaca gccgccacgg caagaacctc gtcggggcct ttcaccagcc cgcgctggtg
     4741 ctggccgaca ccgccgtgct cgatacgctc gggccgaggg agttccgcgc cggctatgcc
     4801 gaggtggcga aatacgggct gatcgaccgc ccggactttt tcgagtggct ggagtcgggc
     4861 gcctggtccg gcatcttttc cggcgggcct gagcgcgagc gggcggtcga gatctgctgc
     4921 gccgccaagg cggccgtcgt cgcccgggac gagaccgaga ccggagaccg cgccctcctg
     4981 aacctcggcc acaccttcgg ccatgccatc gaggcggtga cgggctatga cgcccgtcgc
     5041 ctggttcatg gcgagggggt cgccatcggc atggcgctgg cgcatcgctt ttcccagcgg
     5101 ctgggcctgt gcccgggaca ggacgcgttc cgtgtttcaa ggcatctgga ggcggtcggc
     5161 ctgcccacgc gcctggccga agtgccgggc gggatcggat cggccgaggc gctgctcgcc
     5221 gcgatccggc aggacaagaa ggtcagccga ggcacgctga ccttcatcct gacgaacggc
     5281 ctcggccgga gcttcatcgc caggaatgtg gccgaaaccg aagtcgccga cttcctcgcc
     5341 gaggagctgg cgggctgagg ctggatcgcg cgtcgccccg ccctttcggt cagctcgcga
     5401 agacctcgcc cttggcatcg ggcgggcgcg ccacgccttc cgtcccggcc atgaactgtg
     5461 ccttggccag cgtggcaaac cggccgccct gcgccaccag cgtctcgaag gtcccgctct
     5521 ccacgatacg gccctgctcg aagacgagga tgcggtcggc attgcggatc gtcgccagcc
     5581 ggtgcgcgat gacgaatgtg gtccgccccc gcatcaccgc atcgagcgcg gcctgcagtt
     5641 tctgttcggt cgccgcgtcg agggcgctgg tcgcttcgtc gaggatcagc agcggcgggt
     5701 tcttcagaag cgcgcgcgcg atcgacaggc gctggcgttc gcccccggac agcgagcggc
     5761 cgcgctcgcc gatcacggtg tcgaggccgt ccggctgccg cttgatgaaa tccagggcct
     5821 gtgcccgctc cagcgccgac atcatctcct cgtcgctggc gtccggtctg ccgacctgga
     5881 ggttttcccg gatcgatcgg gcaaacagca tcggttcctg gaacaccacg gcgatgttgc
     5941 gccggagcga ggtcagcgac acgtcccgga tgtctcgccc gtcgatcagg attcgccccg
     6001 actgcggatc gaaggcccgg tgcaagaggc cgagcgtcgt cgacttgccc gagccggtcg
     6061 ccccgaccag cgccacggtc tgtcccttgg ggatgtcgaa gctgacgtcg gcgaccgcgg
     6121 accgcttgcc gtcgtaggag aaggagacgg tgtcgaaggc gaccgcgccg tccatgcggc
     6181 ccgcgtcgat cgcccccggc ttgtcgtgca cggccggtgt cgtgtcgagg acctcgaaga
     6241 actcctccat cttcggcgcc tgcatgaaca gcgagttcac gaatccgacc gcggcctcaa
     6301 gcttgccgat cagcagcgtg gcatagccca tgaaggtgac gatatcgccg atgctggcga
     6361 ggccgttgag gtgcagccag gtgccgagca ggaagatgga cgtcacggtc agtgtcgagg
     6421 cggcgcgggt cgcgaccgac gccatcgccc accaggacag caccgggatc tgcgccgcca
     6481 gcagccggtc gatgatgtcg cgcagcgaat gcagttcggc ctgcacgcgc gtgaagctct
     6541 ggatcaccgg gatattcccg agcgcgtcgg aggcatgttc ggcaaggtcg gtatggtagc
     6601 gctccaccgc gccctgcttc gattccgtct tgcgcagcac gacggccgtc agcgcggcga
     6661 agaccacgac cagcaccacc agcagcaggc cgagccgcca gttgatgaac agtgtgaacg
     6721 gcagcagcac gaaaagtgac agcagcgcgg cgcaatgatc gcggaagaag ccgagccaga
     6781 gccaggccat tccgttcgag ccttcgagca tcaccttcag cacgcggccg gaatgggtct
     6841 gggtgtggaa ggacaggggc agcgccagca cgtgttcgaa gaagccggcc atgacgccga
     6901 ggcgccggcg gtgcgagagg cggtcggcat gcagcgccac cagcacgcca ccgccgatgg
     6961 tgaacaggcc gaagaacagc caggccatga tcagcggcgt gagcgtctcg aaggtgggct
     7021 tggcctggcc ctgcgccccg gtcagcacgt tgatgatgcg cccgaacaga tagggttcgg
     7081 cgaactgggc gatggacagc gccaggttgg cgaacagcag caggacgccg atcttcagtt
     7141 cggcgccaag ctgtccgacg acccgggcat agagacgaaa cagcgacatg aacgagccct
     7201 ttccgccccg caataagcgg cgagcagcct taagggcaag cgctatgaac gggaactgaa
     7261 tccgccgcca tcaggcggcg gttgtcgccg gaacggtgcc gttgaatttc tggatcaggg
     7321 caccgagatc atcgcgacgc accggacggc tgtagaaata gccctgcact tccgaacagc
     7381 ccatttcccg gatcatgttc aggacttcct ccgtctcgac gccctccgcg gtcgtcgtca
     7441 tgccgagcat gtgtccgaga ctggcgaccg agcgcacgat cgccaggcag tccggccggt
     7501 ttccaagctc gcgcacgaag gactggtcga tcttgatctt gtcgaacggg aagctgcgca
     7561 ggtagctcag cgacgaatag cccgtgccga agtcgtccag cgaaatgcgc acgccgagcg
     7621 cgcgcagttg atgcagcacc tcgaccgtga agtccatgtc ctgcaccagc gcggtctcgg
     7681 tgatctccag ttccagccgg cctggttcca ggccagtttg ttccagcgcg ctccgcacgg
     7741 tgccgacgag gttgcggtcg cggaactgca cggcggagag attgacggca atcttgatgc
     7801 cctgcggcca gttcagcgcc tcggcgcagg cgcgccggag cacccattcg ccgatcggca
     7861 cgatcaggcc gacttcctcg gcgatcggga tgaactcgcc cggcgagatc atgccgcgct
     7921 cggaatggcg ccagcggatg agtgcctcga accccgagat gcgggtgttg gaaagcgtga
     7981 ccagcggctg gaagtgcagt tccagctcgt tgttggggat ggcgcgcttg agatcgacct
     8041 cgaccagacg gcgcgcctgc agcttggcgt ccatgcgcgc ctcgaagaag tgataggcac
     8101 cgcggccctc ttccttcgaa tgataaagcg caaggtcggc gttcttgagt agctgctcgg
     8161 agctggcgcc gtcctggaag ccgatcgcga tacccacgct ggtgccgacc gagacctcgt
     8221 tgccgtccag ctggaacggc gtcgagatat ccttcaccag acgcgcaccg aggacatgca
     8281 ggaccgtcgg atccgacaga tcgttcttga ggatcgcgaa ttcgtcgccg ccaaagcggg
     8341 ccaccacgtc acccttgtcc accgcagcct tgagccgcgc cgaaaccgcc ttcagcaaga
     8401 ggtcgccgac aggatgcccg agggtatcgt tgacagcctt gaatcggtcg agatcgagac
     8461 agagcacggc gcagccggcg tcgccacggc gcgccttggt gagcgcgtct tccagatgct
     8521 tcaggaagaa ggcccggttc ggcagcccgg tcagcgcgtc gtgatgcgcc atgtgggcga
     8581 tctgttcctc ggtgcgcttg cgctcggtga tatcctcgta ggtcgccacc cagccgccat
     8641 cgaccatcgg ctggtgatag atcgcgaaga ttcggccatc ggcgtattcg cgcgtgaacg
     8701 acgaggcgct gcggttggaa accagcgctt cctgttcgcg cagcagttcc agcgtggcct
     8761 cgtcgccaag cttcagcgct atgccgatgc gcacgagcaa cgcgttgagg gcggtgcccg
     8821 gacggacagt ctcggcgtcc agatagaaca attcccggaa ctgctggttg cagacgatca
     8881 gcttgctgtc agcgccgacc atgcacagcc cgtgcgacat gttgttgagc gcggcatcga
     8941 agcgcagatt ctgcgtccgc aattccgcat cgcgcaactt caggatctgg ttgtggaggt
     9001 tgagctcggc gttggccgtc tgcgtaccct ggttcgctgt ctcgaggtcg atcttggtcg
     9061 cctcaaggtt tgcggccagt tccttcaact cgccgttggt gcgcttcaag accttgaagt
     9121 tccagaacat catcgccatc agggccatgc cgcaggcgat gatgccgagc atcaggatcg
     9181 aaaaggtcca gtgcaggcgc agcagcgcat gctgatcctc gtcgaccctg tctccgccga
     9241 agacattcgc ggccgcggca aggcgcgaca gccttttgtc gagcggcgtc agcatgcgca
     9301 tcagccgcag cacgttctct ggctgcttgt cgagcgcatc gatcagcggt tgcgccgctt
     9361 cgatcgtctg ggtcagttcc tcgacgatct cctggcgctc gcgatcctgc gagacgaagt
     9421 cattgaactc gccgcccttg atcagcttca tgcggctctg cacgatgtcg agccgaagct
     9481 ggacctcttc ctgatccacg ccgctcttgg gcacgccgaa ggcgttgacg acctggacca
     9541 ggcgggcaac ctcgacgctc gcctgggctg ccagccaggc gatgttgtaa cgggccgatt
     9601 tctggatggt ctgctggcgt tcgccgatca gcgacgacgt gtagaccgcc gcaaacagga
     9661 agaacgccat cagagcgacg gctaggaatt ggaagacgcg aagcagtttc atccatcggc
     9721 ccgggaatgc gaccggcccc tccgtgactg gtgaggccgg gcttggtgcg gcggtttggt
     9781 ccgtcttact tcacggtcag ccgggcgacc tgccaggccg agcggctgta gaacttctgg
     9841 ctcttcagtt ccggatcgct gtcatacgga tagacaatga acagcggacc cttgtccttc
     9901 accggcatgt actcgccgtt gcgcttcagc gcgaggatcg gcttgtactt ggcgaaatcc
     9961 tcgatcggga tctcggttga gtagtcgttc agcgcaaccg cgacgagccg gtcgcccttg
    10021 gcgccgatcg ccttcatcag ggactccatc ggaatgccct cgaatttcat cgtgccggaa
    10081 taccacggcg tcgtcgtctc gatcgtcgtc atgccgagct tctcgagcat gccgcggtcg
    10141 aactgcgccg tgtcgtcaac gttggtgacg ccgatcttgc cgttgaccgt gagcacgatc
    10201 ttctcctgcg gcgctttcag gtctccggcg ctggccggaa gggacaggcc catgccgcct
    10261 gccgcggcca ggaccagtcc aaagtctcgt ctgctgagca tgtcgttccc cacgttttgc
    10321 cctggatacc catgtatgca gttcggaaag tgccggacat gtttgaagga ataattaacg
    10381 gtgttgggaa agaatcccgt tcaggggaga acggccgccc gtgctggccg tcgggtctgc
    10441 aagatgagag gctcccatct cagtccttga ttgagttttt ccatgtacac aatcgtcagt
    10501 ggcgctgatt tcattaacca ttcacataaa ctatttaaaa gttgaattat cggatcatgc
    10561 ttgcgaagat gcgggtctgg agcctggcgg ttcaccgaca ttagagtggg gcaataatga
    10621 aatcgaatgt actgcgcctg aatgcggcgc gcctgtgcaa cgagacggtc gagggattca
    10681 cgaccgcctc cgagcgcaat gccctcaagg ttgcggccgc gcagaagatc gaggagcttt
    10741 tcgacatcct caagatcgac tatcgcaacg accacaacac gcgggagacg ccgcagcgcg
    10801 tcgccaagat gctcgtggag gagacgcttt cagggcgcta caacgagcct cccaagatca
    10861 cggatttcga gaacgcccag aactatgacg aactgatcgt cacgggcccg atcgaggtgc
    10921 gctcgacctg cgcccatcac atgatgccga tctacggctc ggcctacatc ggcgttctgc
    10981 cagcgacgga cggcaagatc atcggcctgt ccaaatacga ccgcatcgtc gattacttct
    11041 cctcgcgcct gcagatccag gaggagctgg tcaagcagat cgagaagttc atcgtcgaga
    11101 agacgcagcc gcgcggcctc gcggtccgca tcagcgcggt gcatatgtgc aagacccagc
    11161 gcggcgtgcg cgccagccat caaagccgga tggtcaactc gaccttctat ggcgagatgt
    11221 cccgcaacca gtcgctccgg gcggaattcc tgcaggaatg cgcggtcctc gacgcgcgtt
    11281 gaaggcgaat cgcccggaat ttcccgctca gctcccgccg cgcggttcgt tcagacttcg
    11341 gcggcgctga cgaccagcgt ggccccgtcg acacccgtca ccctgacctg cgcgcccttg
    11401 tccagcgccg ggccgctgac gcgccagagc gtatcgtcga tgcgcacccg gccaaaaccg
    11461 tcgacgatcg cctcctccag cgcgaaccga cggccgatca gttgcgaact gcgatcatgc
    11521 agcgtctccg ccgcgtccgt cgcccggtcg cggccatggt gcagtttcca cgcgaggaaa
    11581 ctcgcggcca gcgccaggag cccgaacacc acgagttcca gctgccagct tatcgcgacc
    11641 agcgccacga tggcgcccat caccagcgcg gcgatgccga tccacaacag gaatccgccc
    11701 gggatgacca gcacctcgag cgcgatcagc agcaacccga gggcgagcca gccccaagat
    11761 cccagtgagc ccatcatgtc agtttactcc gtgcgtcctg ccgcaggcac g
//
LOCUS       DAOMXN010000053        11616 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_2986_length_11616_cov_13.566733, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000053 DAOMXN010000000
VERSION     DAOMXN010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 11616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11616
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2986_length_11616_cov_13.566733"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1416)
                     /gene="nuoN"
                     /locus_tag="PK812_05925"
     CDS             complement(<1..1416)
                     /gene="nuoN"
                     /locus_tag="PK812_05925"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015930666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HRJ69128.1"
                     /translation="MSATAATIPALGPVLPELVLAIAALVLVLVGAFRGDAGKRLVDV
                     IALAALFLAGFLVLDQGAATQVTFNGAFVSDAFARFMKILTLVGAIVALILSSSFLRE
                     EKINRFEYPILIVLSTLGMMVMISANELISLYLGLELQSLALYVLAAIHRDNAKATEA
                     GLKYFVLGALSSGMLLYGMSLIYGFTGSVTFPTVAKALQGAHGAGVVFGLVFVMAGLA
                     FKISAVPFHMWTPDVYEGAPTPVAAFFASAPKMAAVAIFVRLMVDAFPGITREWQPIV
                     SFIAIASMGLGAFAAIGQSNIKRLIAYSSIGNMGYGLVGLAAGTVQGVEGIAIFMAIY
                     LAMTLGAFACILAMKTEKGRVEDIADLSGLSRTSPMMAFMLAMILFSLIGIPPLAGFF
                     GKFYVFAAAIQAKLYPLAVIGILTSVVAAFYYLRVIKVMYLDEPRAAFLPAAIEVRAV
                     LGIAGLFILLFWIFPGPAMKDA"
     gene            complement(1437..2945)
                     /locus_tag="PK812_05930"
     CDS             complement(1437..2945)
                     /locus_tag="PK812_05930"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HRJ69129.1"
                     /translation="MFGFGILSGLTFLPLVGAAFVLLQRDDAAGLRNARWAALWTTLV
                     AFVLSLVAWGRFDTAKAGFQLLEKKAWFGTEMVYQMGVDGISMPFVLLTTLLMPLCIL
                     ASWESIQTRVREYLAAFLVLETLMIGVFTALDLVLFYLFFEGGLIPMFLIIGIWGGKR
                     RIYASFKFFLYTLVGSVLMLLAIMAMYWDAGTTDIVRLMDHKFAREMQTWLWLAFFAS
                     FAVKMPMWPVHTWLPDAHVEAPTAGSVILAGILLKMGGYGFLRFSLPMFPAASAQFAD
                     LVFILSCIAIVYTSLVALMQEDIKKLIAYSSVAHMGFVTMGIFAFNQQGVQGAVFQMV
                     SHGLISGALFLCVGVVYDRMHTREIAAYGGLVEKMPIYAVIFLLFTMANVGLPGTSGF
                     VGEFLTLAGAYKASTWVAFVATTGVILSAGYALWLFARVVFGTMTKPALAHIPDIDRR
                     ELAVLLPLVILTVYYGVQPGPILEACAASVELVVKQTLAGIGPVKSALSSLY"
     gene            complement(2946..5024)
                     /gene="nuoL"
                     /locus_tag="PK812_05935"
     CDS             complement(2946..5024)
                     /gene="nuoL"
                     /locus_tag="PK812_05935"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HRJ69130.1"
                     /translation="MYTAIVFLPLLGFVLAGIASLLAHKLARPGEVAAFGAADHSAHA
                     EHGHHDHHGHDDHDHHHAPPSPHSAGAIKFAEFVTTGLLFVSAFLSWIAFFSIAMGKG
                     ETVRITIAKWIVSGGMSVDWALRVDPLTAVMLVVVTTVSALVHLYSMGYMHEDPHRPR
                     FFAYLSLFTFAMLMLVTADNLAQMFFGWEGVGLASYLLIGFWFRKPSANAAAIKAFVV
                     NRVGDFGFALGIFLVFWTFGTISFDPIFAGVPGQASATTKFLGYEIHTLTLACCLLFM
                     GAMGKSAQFLLHTWLPDAMEGPTPVSALIHAATMVTAGVFMVARLSPLFEYAPFAQSV
                     VIIVGATTAFFAATVGLVQNDIKRVIAYSTCSQLGYMFVALGVGAYSAGIYHLFTHAF
                     FKALLFLGAGSVIHAMHHEQDIRKMGGLWSKIPFTGAMMLIGTLALTGFPFTAGFFSK
                     DAIIEAAYASHRTGHVYAFLLTVAAAFMTSFYSWRLFFLTFKGETRADKHTYDHAHES
                     PMTMLVPLAVLATGAIVAGYPFVQKFIGHDFDTFWKGAIYMAKYNHILHDMHAVPFWV
                     KAIATVMMVAGFVLAWAFYIQKPERPKQLAAAFPQAYQFVLNKWYFDELYDLIFVRPA
                     KALGRFFWKKGDGWLIDGFGPDGISALSLKVADRATKIQTGYVYHYAFAMLIGVAALV
                     TWYLFAGGVH"
     gene            complement(5033..5341)
                     /gene="nuoK"
                     /locus_tag="PK812_05940"
     CDS             complement(5033..5341)
                     /gene="nuoK"
                     /locus_tag="PK812_05940"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HRJ69131.1"
                     /translation="MDIGLTHYLVVSGILFTLGVFGIFINRKNVIVILMSIELILLSV
                     NINFVAFSTALGDLTGQVFALLVLTVAAAEAAIGLAILVVYYRNRGSIAVEDINMMKG
                     "
     gene            complement(5344..5958)
                     /locus_tag="PK812_05945"
     CDS             complement(5344..5958)
                     /locus_tag="PK812_05945"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HRJ69132.1"
                     /translation="MNAIAFFFYLFSAVLLASAFMVIASRNPVHSVLFLILAFFNAAG
                     LFLLMGAEFLAMILVVVYVGAVAVLFLFVVMMLDVDFVELRQGFLKYLPIGALVAIVF
                     AIEMALVLGTWKMAPGAVEMGKAAAKAPIEAMNVRALGRVLYTEYVYFFQLAGLILLV
                     AMIGAIVLTLRERVGVKRQDIMRQNARTKATAMEVKQVRPGQGV"
     gene            complement(5970..6461)
                     /gene="nuoI"
                     /locus_tag="PK812_05950"
     CDS             complement(5970..6461)
                     /gene="nuoI"
                     /locus_tag="PK812_05950"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HRJ69133.1"
                     /translation="MSVIAQAAKGLLLKEFVSAFFLAMRYFFKPKATLNYPFEKGEIS
                     PRFRGEHALRRYPNGEERCIACKLCEAICPAQAISIEAGPRRNDGTRRTTRYDIDMTK
                     CIYCGFCQEACPVDAIVEGPNFEFATETREELFYDKDRLLDNGARWEREIARNIAMDA
                     PYR"
     gene            complement(6474..6719)
                     /locus_tag="PK812_05955"
     CDS             complement(6474..6719)
                     /locus_tag="PK812_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69134.1"
                     /translation="MQVSLYGLGMAGLLGIGAFLARRVMLKALDNAEKADAKTLSPEQ
                     MAEKRKVRGVVENVLIAELLIMPILGYWIGHTYLTPR"
     gene            complement(6729..7868)
                     /gene="nuoH"
                     /locus_tag="PK812_05960"
     CDS             complement(6729..7868)
                     /gene="nuoH"
                     /locus_tag="PK812_05960"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HRJ69135.1"
                     /translation="MTDVSSSRKNSGGIAPLWIYAGFAIVALVGIAIAAWVVWWLFTQ
                     TTWFVPVLIMALKSVVLLVVLLVLIAFFLLADRKIWAAVQLRRGPNVVGPFGLLQSFA
                     DLLKFVLKEPVIPASADKVLFLLAPLVTCILALAAWAVIPAQAGWAIADINVGILYIF
                     AISSLGVYGIIIGGWASNSKYPFLGALRSAAQMVSYEVSIGFVIITVLLVCGTLNLTD
                     IVEAQNNRYGALGWFWLPLFPMFVIFFISALAETNRPPFDLVEAESELVAGFMSEYGS
                     TPYLLFMLGEYVAIMTMCAMTTILFLGGWLPPVNAAPFTWVPGFVWFIGKIAFVFFMF
                     AMAKAMVPRYRYDQLMRLGWKVFLPLSLAGVVLTAGVLQLTGWYR"
     gene            complement(7878..9941)
                     /gene="nuoG"
                     /locus_tag="PK812_05965"
     CDS             complement(7878..9941)
                     /gene="nuoG"
                     /locus_tag="PK812_05965"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008190225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HRJ69136.1"
                     /translation="MTKLSIDGVEVDVPPHYTLLQACEAAGVEVPRFCFHERLSIAGN
                     CRMCLVEVKGGPPKPQASCALGVRDLRPGPNGEPPVVLTKSPMVKKAREGVMEFLLIN
                     HPLDCPICDQGGECDLQDQAMAYGGSRSRYGEDKRAVEEKYLGPLVKTVMTRCIQCTR
                     CVRFTTEVCGVTEMGAIGRGEDMEITSYLEKALTSEMQGNVVDLCPVGALTAKPYAFQ
                     GRAWEFQKTESIDVMDALGSAIRVDTRGREVMRVLPRVNEAVNEEWISDKTRHVQDGL
                     RTQRLDRPYVRENGSLREASWAEAFAVIAAKVKAARPERIGALAGQLAGVEEMFALKA
                     LIGALGSANLDCRYPGSPLHPKNGRASYLFNATIAGIDEADAIMIVGANPRLEAPVLN
                     ARIRKRWLRGDLLVGVIGEKADLTYSYNYLGAGPDSLAEFVSHPPANKQKPMLILGQG
                     ALNREDGAAVYAQAAKAAQALGVIKDGWNGFNVLHTEAALVGALDIGFVPGEGGLDTA
                     GMLKVGALNVLVNLGADEIDVPAGAFVVYIGTHGDKGAHRADVILPGAAYTEKSATYV
                     NTEGRVQMTARASFPPGEAKEDWAILRALSAELGRTLPFDSLNSLRAAIYAVHPHLAA
                     LDQVKAGDAGDIARLSGAGGKPGKAPFASPVTDFYMTNAISRASKIMAECSALAKTRG
                     AVAAE"
     gene            complement(9946..11250)
                     /gene="nuoF"
                     /locus_tag="PK812_05970"
     CDS             complement(9946..11250)
                     /gene="nuoF"
                     /locus_tag="PK812_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010686013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HRJ69137.1"
                     /translation="MLADKDRIFTNLYGFQDPGLKGALKRGAWDGTKYLLEKGRDWII
                     DEMKASGLRGRGGAGFPTGLKWSFMPKQSDGRPHYLVINGDESEPGTCKDREILRHDP
                     HLLIEGAMLASFAMGANACYIYIRGEFIAERVALQKAVDEAYEAKLIGKSNVHGYPFD
                     FYVHHGAGAYICGEETALLESLEGKKGMPRMKPPFPANMGLYGCPTTVNNVESIAVAG
                     TILRRGAGWFSSIGRPNNVGTKLFCVSGHVNKPCNVEEAMGITFRELIDTHCGGIRGG
                     WDNLKAVIPGGSSVRMVPAEQIIDTPMDFDSLSKLRSGLGTAAVIVMDKSTDIVRAIA
                     RISYFYKHESCGQCTPCREGTGWMYRVLNRMAEGRAQKKEIDMLLDVTKQVEGHTICA
                     LGDAAAWPIQGLIAHFRHEIEAKIDAYSANPHSEPARLMAAE"
     gene            complement(11262..>11616)
                     /locus_tag="PK812_05975"
     CDS             complement(11262..>11616)
                     /locus_tag="PK812_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HRJ69138.1"
                     /translation="PPADKAPAKPAAKAPIEKKAKAEDKPPVLEKARDGKADDLKLIW
                     GVGPKLEKMLHGMGIFHFDQIAAWTDKQLVWVDENLEGFYGRAERDDWIGQSKKLASG
                     WRPENAVGDKPAGKK"
BASE COUNT         2360 a         3728 c         3680 g         1848 t
ORIGIN      
        1 cgcatccttc atcgccggtc cagggaaaat ccagaacagc aggatgaaca ggccggcaat
       61 gcccagtacc gcccgcacct caatcgccgc aggcaggaag gcggcgcgcg gctcatccag
      121 atacatcacc ttgatgaccc gcagataata gaaggccgcg accacgctgg tcaggatgcc
      181 gatcaccgcc agcggataga gcttggcctg tatggccgca gcgaagacgt agaacttgcc
      241 gaagaagccg gcgagcggag gaatgccgat cagcgagaac aggatcatcg ccagcatgaa
      301 ggccatcatc gggctggttc gggacaggcc cgaaaggtcg gcgatgtctt caacccggcc
      361 cttctcggtc ttcatggcga ggatgcaggc aaaggctccg agcgtcatgg caagatagat
      421 cgccatgaag atcgcgatgc cttcgacgcc ctgcacggtg ccggccgcga ggccgaccag
      481 cccgtagccc atgttgccga tcgacgaata ggcaatcagc cgcttgatgt tgctctgacc
      541 gatcgccgcg aaggcgccaa ggcccatcga ggcgatggcg atgaaggaga cgatgggctg
      601 ccactcccgc gtgatgcccg gaaaggcatc gaccatgagg cgaacgaaga tcgccaccgc
      661 cgccatctta ggcgcgctgg cgaaaaaggc cgccaccggg gtgggcgcgc cctcgtaaac
      721 gtccggcgtc cacatgtgaa acggcacggc ggaaatcttg aaggccagtc cggccatgac
      781 aaagacgagg ccgaacacga cgccggcgcc gtgggcaccc tgcagcgcct tcgccacggt
      841 cgggaacgtg accgaaccgg tgaagccgta aatcaggctc ataccgtaga gcagcatgcc
      901 cgacgacagc gcgccgagca cgaagtactt caggcccgcc tcggtcgcct tggcattgtc
      961 acgatgaatg gccgcgagca cgtagagcgc aagagactgg agttcgaggc cgagatagag
     1021 cgagatcagt tcgttcgctg aaatcatcac catcatgccc agcgtcgaaa ggacgatgag
     1081 gatgggatat tcgaagcgat tgatcttctc ttccctgagg aagctggatg acaggatcag
     1141 ggcaacgatg gcgccaacca gcgtcaggat cttcatgaag cgggcgaagg cgtcggaaac
     1201 gaaggcgccg ttgaaggtga cctgcgtcgc cgccccctga tcgagcacga ggaagccggc
     1261 gaggaacagc gcggcgagcg cgatcacgtc cacgagccgc ttgcccgcat cgccccggaa
     1321 ggccccgacc agcacaagca ccagcgcggc gatggccagc acaagctccg gcaggaccgg
     1381 gccgagggcg ggaatggttg cagcggtcgc agacatcgga cgcgcgtcct tttcgttcag
     1441 tacaggctcg acagagcgga cttcaccggg ccgatgcccg cgagagtctg cttgacgacg
     1501 agttcaacgg aagcggcaca ggcctcgagg atcggcccag gctgcacccc ataatagacg
     1561 gtgaggatga ccagcggcag gagcacggcc agttcgcggc ggtcgatgtc tggaatatgc
     1621 gccagcgccg gcttggtcat ggtgccgaac accaccctgg caaacagcca gagcgcatat
     1681 cccgcagaaa ggatcacgcc cgtggtcgcc acaaaggcta cccaggtcga ggccttgtag
     1741 gcgccggcga gcgtcaggaa ctccccgacg aagcccgacg tccccggcag gccgacattc
     1801 gccatggtga acagcaggaa gatgacggca tatatcggca tcttctcgac aaggccgccg
     1861 taggccgcga tctcgcgcgt gtgcatgcgg tcatagacga cgccgacgca gaggaagagc
     1921 gcgccggaaa tcagcccgtg gctcaccatc tggaagaccg cgccctgcac accctgctgg
     1981 ttgaaggcga agatgcccat ggtcacgaag cccatgtggg cgaccgagga ataggcaatc
     2041 agcttcttga tgtcctcctg catgagcgcc accagcgagg tgtagacaat ggcaatgcag
     2101 gacaggatga agaccagatc ggcgaactgc gccgaagccg ccgggaacat cggcagcgag
     2161 aagcgaagga agccgtagcc gcccatcttc aagaggatgc cggcgaggat gacggagccg
     2221 gcggtcggcg cctcgacgtg cgcgtccggc agccaggtat gcactggcca catcggcatc
     2281 ttcaccgcga aggaggcgaa gaaggccagc cacagccagg tctgcatctc gcgggcgaac
     2341 ttgtggtcca tcagcctgac gatgtcggtg gtgccggcat cccagtacat cgccatgatg
     2401 gcgagcagca tcagcaccga gccgaccagc gtgtagagga agaacttgaa gctggcgtag
     2461 atgcgccgct tgccgcccca gatgccgatg atgaggaaca tcgggatcag gccgccctcg
     2521 aagaacagat agaacagcac cagatcgagc gccgtgaaga cgccgatcat cagtgtctcc
     2581 agcacgagga aggccgccag atactcacgc acgcgggtct ggatcgactc ccacgaagcg
     2641 aggatgcaca gcggcatcag caaggtcgtg agcagcacga acggcatgga aatgccgtca
     2701 acgcccatct ggtaaaccat ttccgtgcca aaccacgcct tcttttccag aagctggaag
     2761 cccgccttgg ccgtgtcgaa gcgcccccaa gccacaagcg agagcacgaa ggcgaccagc
     2821 gtggtccaga gcgcggccca gcgcgcgttg cgcaggccgg cggcatcatc gcgctgcaac
     2881 agcacgaatg ccgcgccgac cagcggcagg aaggtgagac cggagagaat tccgaagccg
     2941 aacatttagt gcactccccc ggcgaagagg taccaggtga ccagcgccgc gacgccgatc
     3001 agcatggcga aggcatagtg gtagacgtag ccggtctgga tcttggtggc ccggtccgcg
     3061 accttcagcg aaagcgccga gataccgtcc ggcccgaacc cgtcgatcag ccatccatcg
     3121 cccttcttcc agaagaagcg cccgagcgcc ttggcgggcc gcacgaagat caggtcgtac
     3181 agctcgtcga agtaccactt gttgagcacg aactggtagg cctgcgggaa ggcggcagcg
     3241 agttgcttcg ggcgctccgg cttctggatg tagaaggccc aggcgagcac gaagccggcc
     3301 accatcatca cggtggcgat ggccttcacc cagaacggga ccgcgtgcat gtcgtgcagg
     3361 atgtggttgt acttggccat gtagatcgca cccttccaga aggtgtcgaa gtcgtggcca
     3421 atgaacttct gcacgaaggg atagcccgcg acgatcgcgc cggtcgccag cacggccagc
     3481 ggcacgagca tggtcatcgg gctctcgtgc gcgtggtcat aggtgtgctt gtcggcgcgc
     3541 gtctcgccct tgaaggtcag gaagaatagc cgccaggaat agaaggaggt catgaaggca
     3601 gccgcgaccg tcagcaggaa ggcatagacg tggccggtgc ggtgggacgc ataggcggcc
     3661 tcgatgatcg catccttaga gaagaagccg gccgtgaacg gaaagccggt cagcgccagc
     3721 gtgccgatga gcatcatggc gccggtgaac gggatcttcg accacagccc acccattttc
     3781 cggatatcct gctcgtggtg catcgcatgg atcaccgagc cggccccaag gaacagcagc
     3841 gccttgaaga aggcgtgcgt gaacaggtgg tagatgccgg cggaataggc gccgacgccg
     3901 agcgcgacga acatgtagcc gagctgcgaa caggtcgaat aggcgatcac ccgcttgatg
     3961 tcgttctgca ccagtccgac ggtcgcggcg aaaaacgcgg tcgtcgcacc gacgatgatc
     4021 accacggatt gcgcgaacgg cgcgtattca aacagcggcg acaggcgcgc caccatgaac
     4081 acgccggcgg tcaccatggt cgcggcatgg atgagcgcgg acaccggggt cgggccttcc
     4141 atcgcgtccg gcagccaggt gtgcagcagg aactgcgccg acttgcccat cgcgcccatg
     4201 aacagcaggc agcaggccag cgtcagcgtg tggatttcgt aaccgaggaa cttggtcgtc
     4261 gcgcttgcct ggcccggcac gccggcgaag atcggatcga aggagatcgt accgaacgtc
     4321 cagaacacga ggaaaatgcc aagggcgaag ccgaagtcgc ccacgcggtt gacgacgaag
     4381 gccttgatcg ccgccgcgtt ggcggagggt ttgcggaacc agaagccgat cagcaggtag
     4441 gaggcaaggc cgacgccttc ccagccgaag aacatctgcg ccaggttgtc cgccgtcacc
     4501 agcatcagca tggcgaaggt gaacagcgaa agataggcga agaagcgcgg ccggtgcggg
     4561 tcctcgtgca tgtaccccat cgaatagagg tgcacgagcg cggacaccgt cgtcaccacg
     4621 accagcatca ccgccgtgag cggatcaacg cgcagcgccc agtccaccga catgccgccc
     4681 gagacgatcc acttggcgat ggtgatgcgc accgtctcgc ccttgcccat cgcgatcgag
     4741 aagaaggcga tccaggacag gaaggcagaa acgaacagga gaccagtggt cacaaattcc
     4801 gcgaacttga tcgccccggc gctgtgcggc gatggcggcg cgtggtgatg gtcgtgatcg
     4861 tcatgcccgt gatggtcatg atgtccgtgc tcggcatggg ccgagtgatc ggccgccccg
     4921 aaggccgcga cttcaccggg ccgggccagc ttgtgggcca gcaggctggc gatgccggca
     4981 aggacgaagc cgaggagcgg aaggaatacg atggcggtat acatggtccc gctcagccct
     5041 tcatcatgtt gatgtcttca accgcgatcg agccacggtt gcgataatag acgacgagaa
     5101 tggcgaggcc gatggccgcc tccgccgccg ccacggtcag caccagcagc gcgaagacct
     5161 gcccggtcaa gtcaccgaga gccgtcgaga acgcgacgaa attgatgttc accgacaaga
     5221 ggataagttc gatcgacatc aagatgacga tgacgttctt ccggttgatg aagatgccaa
     5281 agacgcccag ggtaaacagg attcccgaga cgacgaggta atgcgtgagc ccgatatcca
     5341 tgatcacacc ccctgccccg gccggacctg cttgacttcc atcgcggttg ccttggtgcg
     5401 cgcgttctgt cgcatgatgt cctgccgctt gacgccgacg cgttcgcgca gggtcagcac
     5461 gatggcgccg atcatcgcca ccagcaggat caggccggcc aactggaaga agtagacgta
     5521 ttccgtatag agcacccggc cgagcgcacg gacgttcatc gcctcgatcg gcgccttcgc
     5581 cgcggccttg cccatctcga ccgcgccggg cgccatcttc caggtgccga gcaccagtgc
     5641 catctcgatc gcgaagacaa tggcgaccag cgcccctatc ggcagatact tcaggaatcc
     5701 ctgcctcagc tcgacgaaat ccacgtccag catcatgacg acgaacagga acagcaccgc
     5761 gaccgcgccg acatagacga cgaccaggat catcgccagg aattccgccc ccatgagcag
     5821 gaacaggccc gccgcattga agaaggccag gatcaggaac agcacggaat gcaccggatt
     5881 gcggctggcg atcaccatga aggcgctcgc cagcagaacg gccgagaaca gatagaagaa
     5941 gaaggcgatc gcattcatcg gttcagcttt cagcggtaag gcgcgtccat cgcgatgttg
     6001 cgggcgatct cgcgctccca gcgggcgcca ttatcgagga gcctgtcctt gtcgtagaac
     6061 aattcctcac gcgtctcggt ggcgaattcg aagttcggcc cctcgacgat ggcgtccact
     6121 gggcaggctt cctggcagaa gccgcaatag atgcacttcg tcatatcgat gtcgtagcgc
     6181 gtggtgcggc gcgtgccgtc gttgcggcgc ggaccggcct cgatcgagat ggcctgcgcc
     6241 gggcatatcg cctcgcacag cttgcaggcg atgcagcgtt cctcgccgtt gggatagcgg
     6301 cgcagggcat gctcgcccct gaagcgcggc gaaatctcgc ccttttcgaa cggatagttc
     6361 agcgtcgcct tcggcttgaa gaagtagcgc atcgccagga aaaacgccga gacgaactcc
     6421 ttgagcagga ggcccttggc ggcttgtgcg atcacggaca tcggaagttc cccttaccgt
     6481 ggcgtgaggt aggtatggcc gatccagtat ccgaggatcg gcatgatcag gagttcggcg
     6541 atgaggacat tctcgaccac gccgcgcacc ttgcgcttct cggccatctg ctcgggtgac
     6601 agcgtcttcg catccgcttt ctcggcgttg tcgagcgcct tcagcatcac ccggcgcgcc
     6661 aagaacgcac cgatgcccag aagccccgcc atgcccaatc cgtagagtga aacctgcatc
     6721 gctccccctt accgatacca gccggtgagt tgcaggactc cagcggtcag caccacgccc
     6781 gcgagcgaga gcggcaggaa caccttccag ccgaggcgca tcaactggtc gtagcggtag
     6841 cgcggcacca tcgccttcgc catggcgaac atgaagaaga cgaaggcgat cttgccgatg
     6901 aaccaaacga agccaggcac ccaggtaaag ggcgcagcgt tcaccggcgg cagccagccg
     6961 ccgaggaaca ggatcgtcgt catggcgcac atcgtcatga tcgccacgta ttcgccgagc
     7021 atgaacagga ggtacggcgt cgatccgtat tccgacatga agccggcgac cagttccgac
     7081 tcggcctcga ccagatcgaa cggcggacga ttggtttcgg ccagcgccga gatgaagaag
     7141 atcacgaaca tcgggaacag cggcagccag aaccagccca gcgcgccgta gcggttgttc
     7201 tgcgcctcga cgatgtcggt caggttcagc gtgccgcaga ccagcaggac ggtgatgatg
     7261 acgaagccga tcgagacctc gtaggacacc atctgcgccg ccgaacgcag ggcgccgagg
     7321 aacggatact tcgagttcga ggcccagccg cctatgatga tgccgtagac gccgagcgag
     7381 gagatcgcga agatgtagag aatgccgaca ttgatgtcag cgatcgccca tcctgcctgc
     7441 gccgggatga ccgcccaggc cgccagcgcc agaatgcacg ttaccagcgg cgccagcagg
     7501 aacagcacct tgtcagcgga ggccgggata accggctcct tcagcacgaa cttgagcagg
     7561 tccgcgaagg attgcagcag gccgaagggg ccgaccacgt tgggaccgcg ccgcaactgc
     7621 accgccgccc agatcttgcg gtccgccagc aggaagaacg cgatcagaac gagcagaacg
     7681 accagcagga cgacgctctt cagcgccatg atcagcaccg gcacgaacca ggtcgtctgg
     7741 gtgaacagcc accagaccac ccaggccgcg atcgcgattc cgaccagcgc cacgatggcg
     7801 aagccggcgt agatccacag cggtgcgatg ccgcctgaat tctttcggga agacgagacg
     7861 tcggtcattc ctgtccccta ttccgccgcg accgcgccgc gcgtcttcgc cagggccgag
     7921 cattcggcca tgatcttgga agcgcgcgaa atcgcgttcg tcatgtagaa gtccgtcacc
     7981 ggcgaggcga agggcgcctt gcccggcttg ccgccggctc cagaaagcct ggcaatgtcg
     8041 ccggcgtcgc cggccttgac ctggtcgagc gcagcgagat gcgggtgcac tgcatagatc
     8101 gccgcgcgca agctgttcag cgagtcgaac ggcaaggtcc gtcccagttc cgccgacaga
     8161 gcgcgcagga tcgcccaatc ctccttcgcc tcgcccggcg ggaaggaggc acgggccgtc
     8221 atctgcacgc ggccttcggt gttcacatag gtcgccgatt tctcggtata ggccgcgccg
     8281 ggcaggatca catcggcgcg atgcgcgccc ttgtcgccat gggtgccgat gtagaccacg
     8341 aaggccccgg cgggcacatc gatctcgtcg gcgccgaggt tgaccagcac gttgagcgcg
     8401 ccaaccttga gcatgccggc tgtatcgaga ccgccctcgc ccggcacgaa gccgatgtcg
     8461 agcgcgccaa ccagggcggc ttccgtgtgc agcacgttga agccgttcca gccgtccttg
     8521 atcacgccca gggcctgggc ggccttcgcc gcctgcgcat agacggcggc gccatcctcg
     8581 cggttgagcg cgccctgccc gaggatgagc atcggctttt gcttgttcgc cggcggatgg
     8641 ctgacgaatt ccgccagcga atccggcccg gcgccgagat aattgtagct gtaggtgaga
     8701 tcggcctttt cgccgatgac gccgacgagc agatccccac gcagccagcg cttgcggatg
     8761 cgcgcgttca gaaccggcgc ttcaaggcgc ggattggctc cgacaatcat gatcgcgtcg
     8821 gcctcgtcga tgccggcgat cgtcgcattg aacaggtagc tcgcgcgtcc gttcttcgga
     8881 tgcaggggcg aaccgggata gcggcaatcg agattggccg agccgagcgc accgataagc
     8941 gccttcagcg cgaacatctc ctcgacgccg gccagctgac ccgccagtgc gccgatgcgc
     9001 tccggcctgg ccgccttcac cttggctgcg atgaccgcga aagcctccgc ccacgacgcc
     9061 tcgcgcagcg agccgttctc gcgcacatag ggccgatcga ggcgctgcgt gcgcaagcca
     9121 tcctgcacat ggcgggtctt gtcggagatc cactcctcgt tcaccgcctc gttgacgcgc
     9181 ggcagcacgc gcatcacttc acggccgcgc gtgtcgaccc ggatggccga gccgagcgca
     9241 tccatgacgt cgatgctctc ggtcttctgg aattcccagg cgcgcccctg gaaggcgtag
     9301 ggcttggccg tcagtgcacc gaccggacac agatcgacga cgttgccctg catctcggat
     9361 gtcagcgcct tttcgaggta ggacgtgatc tccatgtcct cgccgcgccc gatggcgccc
     9421 atctcggtca cgccgcagac ttcggtggtg aagcggacgc agcgcgtgca ctggatgcag
     9481 cgggtcataa ccgtcttcac cagcgggccg agatacttct cctcgacagc gcgcttgtcc
     9541 tcgccgtagc gcgagcgcga gccgccgtag gccatggcct gatcctgcaa gtcgcattcg
     9601 ccgccctggt cgcagatcgg gcaatccagc ggatggttga tgagcaggaa ctccatcacc
     9661 ccttcgcggg ccttcttcac catgggcgac ttggtcagca ccaccggcgg ttcgccgttc
     9721 gggccaggac gcaggtcgcg cacgccaagc gcgcaggagg cctgcggctt gggcgggccg
     9781 cccttcacct cgacgaggca catgcggcag ttgccggcga tggaaagccg ctcatggaag
     9841 cagaaacgcg gcacttccac gcccgccgcc tcgcaggcct gcagcagcgt gtagtgcggg
     9901 ggaacgtcga cctcgacgcc gtcgatggag agttttgtca tgctgtcact ccgccgccat
     9961 gagccgcgcc ggctcggaat gcgggttggc cgaataggcg tcgatcttcg cctcgatctc
    10021 atggcggaaa tgggcgatca ggccctggat cggccaagcc gccgcatcac ccagcgcgca
    10081 gatcgtgtgg ccttcgacct gcttggtcac gtcgagcagc atgtcgatct cttttttctg
    10141 cgcccggcct tccgccatgc ggttcagcac gcggtacatc cagcccgtgc cttcgcggca
    10201 gggcgtgcac tggccgcagc tctcgtgctt gtagaaatag gagatgcgcg cgatcgcccg
    10261 gacgatgtcg gtcgacttgt ccatgacgat cacagccgcc gtgccgaggc ccgagcgcag
    10321 cttggacagc gagtcgaagt ccatcggcgt gtcgatgatc tgttcggccg gcaccatgcg
    10381 cacggacgat ccacccggaa tgaccgcctt cagattgtcc cacccgccgc gaatgccgcc
    10441 gcaatgcgtg tcgatcagct cacgaaaggt gatgcccatc gcctcttcga cgttgcaggg
    10501 cttgttgacg tgcccggaaa cgcagaacag cttggtgccg acgttgttag gccggccgat
    10561 ggaactgaac cagcccgcgc cgcgcctgag aatcgtgccc gccaccgcga tggactcgac
    10621 gttgttcacc gtcgtcgggc agccataaag gcccatgttc gccgggaacg gcggcttcat
    10681 gcgcggcatg cccttcttgc cttcaaggct ttcgagcagc gccgtctcct cgccgcagat
    10741 gtaggcacct gcgccgtgat gcacataaaa atcaaacgga tagccatgga cgttcgactt
    10801 gcctatgagc ttggcctcat aggcctcgtc caccgccttc tgcaacgcca cgcgctcggc
    10861 gatgaactcg ccgcgaatat agatgtagca ggcgttcgcc cccatggcga aggaggcgag
    10921 catcgcgccc tcgatcagca gatgcggatc gtggcgcagg atctcgcgat ccttgcaggt
    10981 gccgggctcc gattcgtcgc cgttgatgac gaggtaatgt ggccggccat ccgactgctt
    11041 cggcatgaag gaccacttca gcccggtcgg gaagcccgcg ccgccgcgcc cgcgcaggcc
    11101 cgacgccttc atctcgtcga tgatccagtc ccggcccttt tcaagcaggt atttcgtgcc
    11161 atcccaggcg ccacgcttga gcgcgccctt caggcccgga tcctgaaagc cgtagaggtt
    11221 ggtgaaaatg cggtccttgt cggcgagcat ggtccccacc cttacttctt gcccgcaggc
    11281 ttgtcgccaa cggcattctc cggccgccag ccggacgcaa gcttcttcga ctggccaatc
    11341 caatcgtcgc gctcggcacg gccgtagaag ccttcgagat tctcatcaac ccagacgagt
    11401 tgcttgtccg tccaggccgc gatctggtcg aaatggaaga tgcccatgcc gtggagcatc
    11461 ttttccagct tcggcccgac accccagatc agcttgagat cgtccgcctt gccgtcgcgg
    11521 gccttctcca gcaccggcgg cttgtcctcg gccttggcct tcttctcaat gggcgccttg
    11581 gcagccggct tcgccggggc cttgtcggcc ggcggc
//
LOCUS       DAOMXN010000054        11451 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_3044_length_11451_cov_12.357494, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000054 DAOMXN010000000
VERSION     DAOMXN010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 11451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11451
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3044_length_11451_cov_12.357494"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="PK812_05980"
     CDS             <1..403
                     /locus_tag="PK812_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell envelope biogenesis protein TolA"
                     /protein_id="HRJ69139.1"
                     /translation="PPPGPRVEGEENIPGRKPKTFAAPRGGTADDLKRIRGIGKQNEG
                     RLHALGIWHFDQIAAWVKDEIEWVGSYLAFPGRIEREEWVSQAKVLATGSETEFSRRV
                     ARGEVATSKDEGARGQDNVADLTGVKGKPKP"
     gene            complement(456..1331)
                     /locus_tag="PK812_05985"
     CDS             complement(456..1331)
                     /locus_tag="PK812_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRJ69140.1"
                     /translation="MQSWQDIQTTSKEGLRLYARRYGDPLADGLPVVCLPGISRNSAD
                     FHELAEHLAAGGTRAVYALDYRGRGRSDYDPDWRNYDIRVELGDVLDTLVALQVSEAV
                     FVGTSRGGLITMVTPMLRPGLVKGAVLNDIGPVIDGKGLARIKSYVGKMPMPRTFREG
                     GEILRQVSGAQFPKADEATWTRYAERTWKDDGKGGLVLQYDSNLLKTLENIDLEQPLP
                     TLWPQFESLEHAPVLTVRGANSDILSQATAEEMTRRHPACALHVVPDEGHAPLLADAP
                     TLRRIAEFLVETEKL"
     gene            complement(1408..3318)
                     /locus_tag="PK812_05990"
     CDS             complement(1408..3318)
                     /locus_tag="PK812_05990"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRJ69141.1"
                     /translation="MTEYRAGRLTEGDAIAARVEDDAARAALEWSAIRLGRQAVGFAR
                     IARFWSQFSDFPMAGWVRRRAEEALFVEQQMPARVLAFFKDREPEMAAGKVALARVLT
                     AQGQAEKANALVRSAWRENRSSQSIRAAIQKEFPDAISAADIRHLAEKLIYRGESAEG
                     LRIATLAGSAAVAQANILAASINETGGALALVQKLEPAQQGEAPAVFARVQHFRRAGQ
                     IATATQAMLNAPRDVDKLGDADEWWVERRMMVRKALDSGDPASAYRIAAEHSAVAPHN
                     RVDAEVHAGWVALQYLGKPDVAARHFAQARREAKTPHSIARAAFWQGRAADAAGDGAS
                     TVFYTDAARHSHVYHGQLARARLGSTALLDTLDEPPVEELEAATRHIGLRAIRALLDG
                     DARDLAQPLIAEAANTASTQAAAIAVGDLAARFGLPRLTLLAGKSAMQRGLKVEEHAF
                     PVFGIPQFETTPDMAEAPIVYAIARQESEFDPNAVSHAGARGLMQLMPETARRTAQRA
                     KLPFDVGSLTANPALNARIGAAHLGELFAEYNGSYILAFAAYNAGGKRVKEWIAAYGD
                     PRDPQIDKIDWIERIPITETRHYVQKVLENVQVYRWRLNRDASTSIIADMTRGARRNR
                     NDLAYTDGPPAP"
     gene            3954..4844
                     /gene="dapA"
                     /locus_tag="PK812_05995"
     CDS             3954..4844
                     /gene="dapA"
                     /locus_tag="PK812_05995"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HRJ69142.1"
                     /translation="MTAHRPFTGSCTALVTHFRDGKLDEKAIRGHVNWQIENGTHGLV
                     PVGTTGESPTLSHDEHKKVVEWCIAESKGRVPVIAGAGSNSTDEAIDFARHAEKAGAN
                     GLLVVTPYYNKPTQEGMYQHFKAINDAVGIPILIYNIPGRSVVDMSVETMKRLFALKN
                     ISGVKDATANMARVSLQRQEIGPEFNQLSGEDATALGFNAHGGHGCISVTSNVAPRLC
                     AQFQEASLKGDTKAALAIQDRLMPLHTALFLETNPSPVKYALSLIRPVTADVRLPMVP
                     CSEGVKKAVEAAMRHAGLLN"
     gene            4844..5314
                     /gene="smpB"
                     /locus_tag="PK812_06000"
     CDS             4844..5314
                     /gene="smpB"
                     /locus_tag="PK812_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HRJ69143.1"
                     /translation="MARAESRYKLVADNRKARYNFEIGQVFEAGIVLTGTEVKSLRTG
                     KATIGESYASVEGDQLFLVNAYIPEYLQANRFNHETKRPRKLLLHRREIDRLVNAVRR
                     EGMTVVPLKLYFNDKGRAKVEIALAKGKKLHDKRETEKKRDWDREKGRLLRDRG"
     gene            complement(5366..5998)
                     /locus_tag="PK812_06005"
     CDS             complement(5366..5998)
                     /locus_tag="PK812_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRJ69144.1"
                     /translation="MAGVDPGRNCPTCPRLVAYRLKARASQPDWHNAPVPTFGGADAR
                     LLIVGLAPGLQGANRTGRPFTGDWAGDLLYETLGAFGFASGTYDERPDDGLKLIDCRI
                     TNAVRCVPPENKPTGAEIAGCRPYLERTIASMPKLAAILALGRIAHESTLRALGEAPA
                     RHVFKHGARHDIGRIALFDSYHCSRYNTNTRRLTPEMFRAVFADIRHSLG"
     gene            complement(6009..6650)
                     /locus_tag="PK812_06010"
     CDS             complement(6009..6650)
                     /locus_tag="PK812_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HRJ69145.1"
                     /translation="MQIERIALFIDGANLYAAARALGFDIDYRKLLDEFRSRGYLVRA
                     FYYTAIVEDQEYSSLRPLVDWLDYNGYMVVTKPAKEFTDAMGRRKVKGNMDIELAVDA
                     MELAGSIEHMVLFSGDGDFRSLVEAVQRKGVKVSVVSTVSTQPPMMSDDLRRQADVFI
                     DLADMQGKIGRAPSERPPREQREFREREPRDPARSMPAFLNRPAQAPRSDEQS"
     gene            6879..7286
                     /gene="rpoZ"
                     /locus_tag="PK812_06015"
     CDS             6879..7286
                     /gene="rpoZ"
                     /locus_tag="PK812_06015"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HRJ69146.1"
                     /translation="MARVTVEDCIDKIDNRFELVLLAAHRARILASGAQPLVKRDNDK
                     NPVIALREIADEQLSPGDLKEEFIHSMQKFVEVDEPEPEAVPRIASPSQAQPSIVANA
                     VDDTNVAFDRMSEEELLRGLDGLVPPSASDDDD"
     gene            7407..9596
                     /locus_tag="PK812_06020"
     CDS             7407..9596
                     /locus_tag="PK812_06020"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HRJ69147.1"
                     /translation="MMRQYELVERVRRYNPHTDEALLNRAYVYAMRAHGHQKRASGDP
                     YFAHPLEVAALLTDLKLDDATIVAAMLHDTIEDTDATREEIDRLFGHEIGALVDGLTK
                     IKKLDLVSKRAAQAENLRKLLLAISDDVRVLLVKLADRLHNMRTLHYVPPEKRARIAE
                     ETLDIYAPLAGRMGMQQMRNELEDLSFRTLMPDAHAMVGKRLAELRERSGELIDVIER
                     TLKAKLDAEGIEAEVKGRQKQPYSIWKKMERKSVGFEQLSDIFGFRIIVDTTENCYRV
                     LGVAHTNWPVVPGRFKDYISTPKQNDYASLHTTVVGPGHQRVELQIRTSDMDDVADQG
                     VAAHAIYKEGSSRALPGDSNAFQWLRKTIQLLAEGDSPEEFLEHTKLELFHDQVFCFT
                     PKGRLIALPRGATPIDFAYAVHTNVGNSAVGCRINGTVAPVVSELKNGDEVEIIRAEQ
                     QTPPAAWESLVVTGKARAAIRRATREAVRRQFAGLGQKIVERAFVRANRAFSMDKLTA
                     ALPRLARQSVEDVLSAVGRGEMYSGDVVKAVYPDFKEEQSRGAQAEHGQGWFELRSAD
                     NLKFKVPGQASTAQSIPIRGLTGELPVQFAPNGGAVPGDRIVGILTPGEGITIYPIQS
                     PALTAFDDEPWRWLDVRWDLEEGGRMRFPAQINLAVINEPGTLAEIAKVIGDTDANID
                     SIAMTSKSADFREMQIDLEVWDLKHLNAIIAELKTKPQVSRVERVNG"
     gene            complement(9631..>11451)
                     /locus_tag="PK812_06025"
     CDS             complement(9631..>11451)
                     /locus_tag="PK812_06025"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ69148.1"
                     /translation="EDEDEDDEEDEEDEDDEDEHPVFTAKEAAGAFTALFRFVWPHIT
                     RYPKWLAIVVFGLLAETAFNVAFPLSLKYLIDDVLTGDSEHAYRTLIIILATLGSLGI
                     VVSIITIWYEYYDSVLMSSVMADIRAKLFEHLQYLSAGFYSRTKIGTITSRFGSDMTT
                     VDDALRHMVTWGVLPFLELLAGIGLMFYLNWQLASLALLLIPITLIGPRLLSGRAVDA
                     SYQERKAAAGLVAVVQENVSAQNVVKAFSLRRISKGWFHDRNMIEREQAIRSRFYNAL
                     VERSVTISVLMLHLVVFGVGAYLTYNEDITLGTFVTFESVFWELSYNIGHVTQFVPVA
                     ISGSGATKHINDLLDEPARGQDPETAAEVPRIQREITFRDVEFSYGGEQKQIEALNMT
                     IPAGSRIAVVGPSGSGKSTVLNLLLRLYDPTGGTIRIDDTDIVKAKRDSLRAQMAVVF
                     QENLLFDISIRENIRLGNPGATDEEVVKAAKAAEIHRFIRSLPQGYDTVVGERGNMMS
                     GGQRQRVAIARAIIRNPAILLLDEATSALDHGTEQAINKTLMKVAEGRTMITVTHRLH
                     SVTDMDKIFVLEKGRLVESGTHAELMKMKGVYAHLFKRAG"
BASE COUNT         2139 a         3592 c         3728 g         1992 t
ORIGIN      
        1 gccgccgccc ggcccgcggg tggagggtga ggaaaacatc cccggccgca agccgaagac
       61 cttcgccgcg ccgcggggtg gcacggccga cgatctcaag cgcatccgcg gcatcggcaa
      121 gcagaacgag ggccgcctcc atgcgctcgg catctggcac ttcgatcaga tcgccgcctg
      181 ggtgaaggac gagatcgagt gggtcggctc ctacctggcc ttccccggcc ggatcgagcg
      241 cgaggagtgg gtgagccagg cgaaggttct ggccacgggc tccgagaccg agttctccag
      301 gcgcgtggca cgcggcgagg tcgccacgtc gaaggacgag ggcgccaggg gccaggacaa
      361 tgttgcagac ctgaccgggg tcaagggcaa gccgaaaccc tgagcggcga tctgccgagg
      421 aatgaccaga cagccccgtt ctggggctgt ttcggtcaga gcttttccgt ttctacgagg
      481 aattcggcga tgcggcgcag cgttggcgcg tccgcgagca ggggcgcgtg cccctcgtcc
      541 ggcacgacat gcagcgcgca ggcggggtgg cggcgcgtca tttcttccgc cgtcgcctgc
      601 gacaggatgt cggaattcgc cccccggacc gtcagaaccg gagcatgttc cagcgactcg
      661 aattgcggcc agagcgtcgg caatggttgt tcaagatcga tattttcaag cgttttcagc
      721 aggttggaat cgtactgcaa taccaggccg cccttgccgt cgtccttcca ggtgcgttcg
      781 gcatagcggg tccaggtcgc ttcatcggcc ttgggaaact gcgcgccgga gacctggcgc
      841 aggatctcgc cgccctcgcg aaacgtacgt ggcatcggca ttttcccgac ataactcttg
      901 atccgggcca gacccttgcc gtcaatcacc gggccgatgt cgttgagcac cgcgcccttc
      961 accagccccg gccgcagcat cggtgtgacc atggtgatca gaccgccgcg cgaagtgccg
     1021 acgaacaccg cctcgctgac ctgcagggcg acaagtgtgt cgagaacatc gccaagttcg
     1081 acccggatgt cgtagttgcg ccagtccgga tcatagtccg aacgcccgcg gccccggtaa
     1141 tccagggcat agaccgcgcg cgtgcctccc gccgcgagat gctcggccag ttcgtggaaa
     1201 tcggcggagt ttcgcgagat gccgggaagg cagaccaccg gcaatccgtc ggccagggga
     1261 tcgccatagc gccgggcata caggcgcagg ccttccttgc tggtggtctg gatatcctgc
     1321 caactctgca tgaaaagctc cgcgatttcc gctggttagg ggaaagtcct ggcaagggta
     1381 ggaggttctc gcagcgccgg caagcgacta tggcgccggc ggtccgtcgg tataggcaag
     1441 atcgttgcga ttgcgccgcg cgccgcgcgt catgtccgca atgatggacg tgctggcgtc
     1501 ccggttcagc cgccagcggt agacctgcac gttctccagc actttctgga cgtagtggcg
     1561 cgtttcggtg attggaatgc gttcaatcca gtcgatcttg tcgatctgcg gatcgcgcgg
     1621 atcgccatag gcggcaatcc attccttcac ccgcttgccg ccggcattgt aagccgcgaa
     1681 ggcgagaatg tacgagccgt tgtattcggc aaagagttca ccgaggtgtg ccgcgccgat
     1741 gcgcgcgttc aaggcaggat tggccgtcag actgccgaca tcgaacggca gcttggcccg
     1801 ctgggcggtt cgacgcgccg tttccggcat caattgcatc aagccgcgcg caccggcgtg
     1861 cgagacagca ttggggtcga attcgctttc ttgccgggca atcgcataga caattggcgc
     1921 ttcggccatg tccggtgtgg tttcgaactg cgggatgccg aatacaggaa atgcgtgctc
     1981 ctcgaccttg aggccgcgct gcatggcgga tttgcccgcg agcagggtca gccgaggcaa
     2041 tccaaagcgc gcggcgaggt cgccaacggc gatcgccgcc gcctgcgtcg aagcggtatt
     2101 ggcagcctcg gcgatcaggg gctgcgcgag atcgcgggcg tcgccgtcga gaagtgcgcg
     2161 gatggcgcgc aatccaatgt gccgtgtcgc cgcttcgagt tcctcaacag gcggttcgtc
     2221 caacgtgtcc agcaaggcgg tgctgcccaa acgtgcccgg gcgagctgcc catggtagac
     2281 atggctatgg cgcgcggcgt cggtgtagaa gacggttgat gcgccatcac cggcggcatc
     2341 ggcggcgcgt ccctgccaga aggctgcgcg cgcaatgctg tgcggtgtct tcgcttctcg
     2401 gcgggcctgg gcgaaatggc gagccgctac gtccggcttg ccaagatatt ggagtgccac
     2461 ccagccggca tgaacctccg catcgacccg gttgtgcggc gccacggcag aatgctcggc
     2521 ggcgatgcgg taggcgctgg ccggatcgcc ggaatccagc gccttgcgca ccatcatgcg
     2581 ccgctcgacc caccattcgt cggcgtcgcc cagcttgtcc acgtcgcgcg gcgcattgag
     2641 catggcctgg gtggccgtag cgatctgtcc ggcgcgccgg aaatgctgga cacgcgcaaa
     2701 caccgccggc gcctcgccct gctgcgcggg ttcgagtttc tggacaagcg ccagggcgcc
     2761 gccggtctcg ttgatgctgg cggccaggat gttggcctgg gccacagctg ccgaaccggc
     2821 cagcgtggcg atccgcaagc cttccgcgga ttcgccacga tagatcagct tctccgcgag
     2881 gtgacggatg tccgcagcgg agatcgcgtc tgggaactcc ttctggatcg ccgcgcggat
     2941 gctctgactg gagcggttct cgcgccaggc cgagcgcacg agagcgttgg ctttctccgc
     3001 ttgcccctgg gctgtaagga cgcgcgccag cgccaccttt cctgcggcca tttccggttc
     3061 gcgatccttg aagaaggcga gcacgcgcgc cggcatctgc tgttcgacga acagggcttc
     3121 ctcggcacgc cgacgcaccc agccggccat cgggaagtcg gaaaattgtg accagaatcg
     3181 ggcaatgcgg gcaaaaccga cggcctgccg ccccagacgg atggcactcc attccagcgc
     3241 ggcgcgcgcc gcgtcatcct caaccctggc ggctatggca tcaccttcgg tcagccgccc
     3301 tgcgcgatac tccgtcaacg ccttcttcag aagctcgaga tcctcggtgc cagctcccgc
     3361 aacgcttggg cgcgccctga cataggcaag ccctgtttcg tctgccggcg ggcttgctgc
     3421 ggcactaacg cgttctttcg ggccgggcag gggcgccggc ggcggagcgg aacgctccgc
     3481 cgtctcttgc gctgccggca gattttgcac aggttttgcc gatgaaaacg cagaaacatg
     3541 ggttgagggc ggagttccca ccgctccgcc agcggaagaa ccggagatcg acgtggagtg
     3601 cgtcgccaga ccgtctggtt ccgctgctcc agcgccaagc gtgacactgc cgggacgccg
     3661 ggtgctgtca aacagcgccg atggcgtcag cacgacagtc agcccgagca ccgtcgcgga
     3721 gaaggcgctg cgcgcatgcg acaggcgggg actttggggc gtcaagcgca tgtccggagc
     3781 ctctgggtga ccctgagcaa accccatcgc cgttaagatc gcgctaacga tgatgggcgt
     3841 gcatgggagc agccaaatcg ctcttttctg cggcgggcct tgccggtatg aggaacgctt
     3901 ggtccgcgca atggcgcgcg gactgtactt cctcttgttc cggacaggcc gacatgaccg
     3961 cccatcgccc cttcacaggt tcctgcaccg cgttggtgac gcattttcgc gacgggaagc
     4021 tggacgagaa agcgatccgc ggccatgtga actggcagat cgagaacggc acgcacggcc
     4081 ttgtgccggt cggtaccacg ggcgaaagcc cgactctgag ccacgatgag cacaagaagg
     4141 tggttgagtg gtgcatcgcg gaatcgaagg ggcgtgtgcc agtcatcgcc ggcgccgggt
     4201 cgaattcgac cgatgaggcg attgatttcg cgcgccatgc cgagaaggcg ggcgcgaatg
     4261 gcctgctcgt ggtcacgccc tattacaaca agccgacgca ggagggcatg taccagcact
     4321 tcaaggcgat caacgacgcc gtgggcatcc cgatcctgat ctacaatatc ccggggcgct
     4381 cggtcgtcga catgtcggtc gaaaccatga agcgcctgtt cgcgctgaag aacatctcgg
     4441 gcgtgaagga cgccaccgcc aacatggcgc gggtcagcct gcagcggcag gagatcggcc
     4501 cggaattcaa ccaactctcc ggagaggacg cgaccgcgct cggcttcaac gcgcatggtg
     4561 gccacggctg catttcggtg acgtccaacg tcgcgccacg cctgtgtgcc cagttccagg
     4621 aggcgagcct gaagggcgac accaaggcgg ctctggcgat ccaggaccgt ctgatgccgc
     4681 tgcataccgc gctgttcctc gaaaccaacc cctcgccggt caagtatgcg ctttcgctga
     4741 tccgtccggt gacggcggac gtccgtctgc cgatggtgcc gtgcagtgag ggcgtgaaga
     4801 aagccgtcga ggcggcaatg cgccacgccg gcctgctcaa ctgatggcca gggccgaaag
     4861 tcgctacaag ctcgtcgcgg acaaccggaa ggcgcggtac aacttcgaga tcggacaggt
     4921 cttcgaggcc ggcatcgtgc tgaccggaac ggaggtgaag tcgctgcgca cgggcaaggc
     4981 caccatcggc gagagctatg cctccgtgga gggtgaccag ctcttcctgg tcaacgccta
     5041 cattccggaa tacctgcagg cgaaccgttt caaccacgag accaagcgcc cgcgcaaact
     5101 gctgctccac cgccgcgaga ttgaccggct ggtcaacgct gtgcgccgcg agggcatgac
     5161 cgtggtgccg ctgaagctgt atttcaacga caagggccgg gccaaggttg agatcgcgct
     5221 cgccaagggc aagaaactgc acgacaagcg cgagaccgag aagaagcgcg actgggatcg
     5281 cgagaagggg cggctgctga gggatcgggg gtaacgcttc cgacagcgcc ggtccgtcca
     5341 aaaagcgcaa aaaagtcgcc gagccttaac ccaggctatg ccggatatcc gcaaataccg
     5401 cccggaacat ctccggcgtc agccgccgcg tattcgtgtt gtagcgcgag cagtggtagc
     5461 tgtcgaagag cgcgatccgg ccgatatcgt gccgcgcgcc atgtttgaag acatggcgag
     5521 cgggcgcctc gcccagcgca cgcagggtgc tctcatgcgc gatgcgcccg agcgcaagaa
     5581 tggcggcaag cttcggcatg ctggcgatgg tgcgttcgag atagggccgg cacccggcga
     5641 tctccgcccc ggtcggcttg ttctccggcg gcacgcagcg caccgcattg gtgatgcggc
     5701 agtcgatcaa tttcagcccg tcgtccggcc gctcgtcata cgtgccggag gcaaagccga
     5761 atgcgcccag ggtctcgtag agaaggtcgc cggcccagtc acccgtgaag gggcgaccgg
     5821 tgcgattggc gccctgcaaa ccgggcgcca gccccacgat gagaagccga gcgtcggcgc
     5881 ccccgaaagt gggcaccggc gcattgtgcc agtccggctg gctcgctcgg gccttcaggc
     5941 gataggcgac gagccgggga catgtcgggc aattgcgccc gggatcaact cccgccacgc
     6001 ccggtgcctc aggactgctc gtcgctgcgc ggcgcctgcg ccgggcggtt caggaaagcc
     6061 ggcatggagc gtgcaggatc gcggggctcg cgttcgcgga actcgcgctg ttcgcgtggc
     6121 ggccgctcgg acggcgcgcg gccgatcttg ccctgcatgt cggcaaggtc gatgaagaca
     6181 tccgcctgcc ggcgcaggtc atcggacatc atcggcggct gggtggagac cgtggacacc
     6241 acggacacct tgacgccctt gcgctgcacg gcttcgacca gagagcggaa gtcgccgtcg
     6301 ccggagaaca gcaccatgtg ctcgattgag ccggcaagtt ccatggcatc gacggcaagc
     6361 tcgatgtcca tgttgccttt caccttgcgc cggcccatgg cgtcggtgaa ttccttggcc
     6421 ggcttggtta cgaccatgta gccgttgtag tcgagccagt cgaccaacgg gcgcagcgag
     6481 gaatactcct gatcctcgac gatggcggta tagtagaagg cgcgcacgag ataaccgcgg
     6541 ctgcgaaatt catccagaag cttccggtag tcgatgtcga aaccaagggc gcgtgctgct
     6601 gcgtagagat tggcaccatc aatgaacagc gcaatgcgtt cgatctgcat tatcaatcct
     6661 tcctcaatcg ggatcagtat cagagtggca aggatggtag tcccatccag aatgcgaagc
     6721 ggaacatggc cggcaatggc caaaagtcaa ggcagggggc tggcggagcc ggaaaagcgg
     6781 cccgtgccgg cattcggtgg tttctgttgc cgaggcggag gaaatcgtat accagcacgg
     6841 ctcctttcac gaaacgcagg ttcgaggagc gccgaggcat ggctcgcgta accgtcgagg
     6901 attgcatcga caaaattgac aaccgcttcg agctcgtcct gctcgcggca catcgtgcgc
     6961 gcatcctggc cagcggcgcg cagccgctgg tcaagcggga caatgacaag aatccggtca
     7021 ttgccctgcg cgagatcgcg gacgagcagc tttcgcccgg cgatctcaag gaggagttca
     7081 tccactcgat gcagaagttc gtcgaggtgg acgagccgga gccggaggcc gtgccgcgca
     7141 tcgccagccc gtctcaggcg cagccttcca tcgtggcgaa cgccgtggac gacaccaacg
     7201 tcgccttcga ccgcatgtcg gaagaggagc tgttgcgcgg cctcgatggg ctggtaccgc
     7261 cctccgccag cgacgacgac gactgacgac gctgccttcg ggcgcattgt acgatttgat
     7321 cgggcgttcc cgcttgcacc gcgggagcgc ccgttccatt ttggtggttc gaccggaaat
     7381 ccccgggccc ttcggaccat aatgtcatga tgcgccaata cgaactcgtc gagcgggttc
     7441 gtcgctacaa tccgcacacc gacgaagcgc tgctcaaccg cgcctacgtc tatgcgatgc
     7501 gcgcgcacgg gcaccagaag cgcgcctctg gcgatccgta cttcgcccac ccgctcgaag
     7561 ttgccgcact cctgactgat ctcaagctcg acgacgcaac catcgttgcg gcaatgctgc
     7621 atgacacgat cgaggacacg gacgcgacgc gcgaggaaat cgaccggctg ttcgggcacg
     7681 agatcggggc gctggtcgac ggcctcacca agatcaagaa gctcgacctc gtctccaagc
     7741 gcgcggcgca ggctgaaaat ctgcgcaagc tcctgctggc gatttcggac gatgtgcggg
     7801 tgctcctggt caagctcgcc gaccggttgc acaacatgcg caccctgcac tacgtgccgc
     7861 cggaaaagcg ggcgcgcatc gcggaagaaa cgctggatat ctacgcgcct ctggccggcc
     7921 gcatgggtat gcagcagatg cgcaacgaat tggaagacct ttccttccgg acattgatgc
     7981 cggatgcgca tgccatggtg ggcaagcgcc tggccgaact gcgcgagcgc agcggcgaac
     8041 tgattgacgt catcgaacgc acattgaagg caaagctcga tgcggaaggc atcgaggccg
     8101 aggtcaaggg gcggcaaaag cagccctact cgatctggaa aaagatggag cgcaagtccg
     8161 tcggcttcga gcagctttcg gacattttcg gatttcgcat catcgtcgac accaccgaga
     8221 attgctaccg cgtgcttggg gtcgcgcata ccaactggcc ggtggttcca ggacggttca
     8281 aggactatat ctcgacgccg aagcagaacg actacgcctc gctgcacacc acggtggtgg
     8341 gaccggggca ccagcgtgtc gagctccaga tccgcacgag cgacatggac gacgtggcgg
     8401 accagggtgt cgcggcccat gccatctaca aggaggggtc atctcgggcg ttgccgggtg
     8461 attccaatgc cttccagtgg ctgcgcaaga caatccagct gctggccgag ggcgacagtc
     8521 cggaagagtt cctcgaacac accaagctgg aactcttcca cgatcaggtc ttctgtttca
     8581 cccccaaggg acggctcatc gccttgccgc gcggtgcgac accgatcgac tttgcctatg
     8641 ccgtccacac caatgtcggg aattccgccg tggggtgccg catcaacggc actgttgccc
     8701 ccgttgtttc cgaactgaag aacggcgacg aggtggagat cattcgcgcg gagcagcaaa
     8761 cgccccctgc ggcctgggag agcctggtcg tcaccggaaa ggcgcgtgcc gccatccgcc
     8821 gcgccacgcg cgaggcggtg cgccgccagt tcgccgggct tggccagaag attgtcgaaa
     8881 gggccttcgt ccgcgccaac cgcgcctttt ccatggacaa gctgaccgcg gccctgccgc
     8941 gccttgcccg ccagagcgtg gaggacgtgc tctccgccgt tggtcgcggc gagatgtatt
     9001 ccggcgacgt cgtgaaggcc gtctacccgg atttcaagga ggagcagtcg agaggcgcgc
     9061 aggccgagca tggccagggc tggttcgagt tgcgctccgc cgacaacctc aagttcaagg
     9121 tgccggggca ggccagcacg gcccagtcca ttccgatccg cggcctgacc ggcgaactgc
     9181 cggtgcagtt cgcgccgaat ggcggcgccg tgcccgggga ccgcatcgtt ggaattctca
     9241 cgcccgggga gggcataacc atctatccga tccagtcacc cgcgctcacc gcctttgacg
     9301 acgagccgtg gcgctggctg gacgtgcgct gggatctgga ggaggggggg cgcatgcggt
     9361 tcccggcgca gatcaatctg gcggtgatca acgagccggg aacactggcg gagatcgcca
     9421 aggtcatcgg cgataccgat gcgaatatcg attccatcgc catgacgtcg aagtccgccg
     9481 attttcgcga gatgcagatc gacctcgagg tctgggacct caagcacctc aatgccataa
     9541 tcgcggaact gaagaccaaa ccgcaggtct cacgcgtgga gcgcgtgaac ggctgatccc
     9601 gggttcagtg ctgcgagtgc cgctgaaggt tcagccagcc cgcttgaaca ggtgggcgta
     9661 gacacccttc atcttcatga gctctgcatg ggtgccagat tcgaccagtc ggcctttctc
     9721 gagcacgaag atcttgtcca tgtccgtgac ggaatgcagc cggtgcgtca cggtgatcat
     9781 ggtgcggccc tcggccactt tcatcaaggt cttgttgatc gcctgctcgg tcccgtgatc
     9841 gagcgcggag gtggcctcat ccagcagcag gatggccgga ttgcggataa tggcgcgggc
     9901 gatggcgacg cgctgcctct gaccacccga catcatgttg ccgcgctcgc cgaccacggt
     9961 gtcgtagccc tgcggcagtg agcgtatgaa ccggtgaatc tccgccgcct tggccgcctt
    10021 gacgacctcc tcatcggtag cgcccggatt gcccagccgg atgttctcgc ggatcgagat
    10081 atcgaacaac aggttctcct ggaacacgac cgccatctgg gcgcgcaggg aatcccgctt
    10141 ggccttgacg atgtcggtgt cgtcgatgcg gatcgtcccg ccggtgggat cataaagacg
    10201 caacagcaga ttgagcaccg tgctcttgcc cgagccggaa ggtccaacca cggcgatgcg
    10261 tgatcccgct ggaatcgtca tgttcagcgc ttcgatctgt ttctgttcgc ctccgtagga
    10321 gaactctacg tcgcggaagg tgatctcgcg ctgaattcgc ggcacctcgg cggccgtctc
    10381 cggatcctgc ccgcgggccg gctcgtccag caggtcattg atgtgtttgg tcgcgcccga
    10441 accgctgatc gcgaccggaa cgaactgcgt cacgtgtccg atgttatagg acagttccca
    10501 gaacacactt tcgaatgtca cgaacgtgcc gagcgtgatg tcctcattgt aggtcagata
    10561 ggcgccaacg ccgaacacga cgagatgcag catcagcacc gagatcgtca ccgaacgttc
    10621 gaccagcgca ttgtagaagc gcgagcggat ggcctgctca cgctcgatca tgttgcgatc
    10681 gtggaaccag cctttcgaga tgcggcgcag tgaaaaggcc ttcacgacgt tttgcgccga
    10741 aacgttctcc tgcaccacgg ccacgagccc ggcggccgcc ttgcgctcct gatagctcgc
    10801 atcgacggca cggccggaca ggagccgcgg gccgatcagc gtaatgggaa tgagcaacag
    10861 ggctaaagag gcgagctgcc agttcaggta gaacatcagc ccgatgcccg cgagcaattc
    10921 caggaagggc agcacgcccc aagtcaccat atggcgcaag gcatcgtcga cggtggtcat
    10981 gtcggaaccg aatcgcgacg tgatcgtgcc gatcttggtg cgggagtaaa agccggcgga
    11041 cagatactgc agatgttcga acagcttggc gcggatatcc gccatcacgg aagacatcag
    11101 cacgctgtcg taatattcgt accagatcgt gatgatcgac acgacgattc caagcgaacc
    11161 gagggtcgca aggatgatga tcagcgtgcg gtaggcatgt tcgctgtcgc ctgtcagaac
    11221 gtcatcgatc aggtacttga gcgacagtgg aaaagcgaca ttgaatgcgg tttcggccag
    11281 caggccgaac acgacgatgg caagccactt cggatagcgc gtaatgtgcg gccaaacgaa
    11341 ccggaagagg gccgtgaagg cgcctgctgc ctccttcgcg gtgaagacgg ggtgttcatc
    11401 ctcgtcgtct tcgtcctcct cgtcctcctc gtcgtcctcg tcctcgtctt c
//
LOCUS       DAOMXN010000055        11361 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_3070_length_11361_cov_9.961348, whole genome shotgun sequence.
ACCESSION   DAOMXN010000055 DAOMXN010000000
VERSION     DAOMXN010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 11361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11361
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3070_length_11361_cov_9.961348"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /gene="dapF"
                     /locus_tag="PK812_06030"
     CDS             complement(<1..663)
                     /gene="dapF"
                     /locus_tag="PK812_06030"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HRJ69149.1"
                     /translation="MLDFVKMHGLGNDFVVLDGRDTPIPVTPDLARAIADRRYGVGCD
                     QLIVLEPSRRADVFMRIYNPDGSQSGACGNATRCVAQVLMREGERDAATIETLAGVLR
                     ASGSPERITVDMGAPRLGWREIPLAHPVADTARVPHDATALKAGLPAWFGAVNMGNPH
                     AVFFVDDVEAHDLARTGPRLEADPVFPERANISLVAVAAPGHLIQKVWERGAGLTLAC
                     GSG"
     gene            798..2108
                     /locus_tag="PK812_06035"
     CDS             798..2108
                     /locus_tag="PK812_06035"
                     /inference="COORDINATES: protein motif:HMM:NF024841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HRJ69150.1"
                     /translation="MRLVILVLSIGIGTSAVPAEESGLVTPIQVPHLPLGLVTLQKTR
                     AINLAVSPGAGAFRLASDPPGRIWTVTDRGPVVDCSEERETIGPDDKAICAPDRRGKI
                     YLLPGYVPSIYALDVNGDGTARFAEMIPLRGKSGKLLSGLTNPLGPQRTETGYTTEGR
                     VLDTDPSGIEPGGLVRLADGTFWIAEKFGPSLLEVARDGQVLRRLVPANLEPELKGAD
                     YAVEPAFPSILSLRAVARGFDAIAISPDERFLYVAMQNALANPALEAYRHSPATRIWK
                     IERTSGRIVAEYVYRLDPPGNFPADTSVAGRPLRQSDVRIAEIVAMGENRLLVLERVR
                     HGSRIFVATLSPGQELPPPYDDEKTRPSLEELFPDVWEIAGIAPVKKMLLVDSERIKG
                     IGGRLTAMAVLSPRELVLMSNNVYGVEGGRSQTFRLTFSAPVFQ"
     gene            2247..2849
                     /locus_tag="PK812_06040"
     CDS             2247..2849
                     /locus_tag="PK812_06040"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HRJ69151.1"
                     /translation="MTTGHQAFGRICAIMLAGVLLAVPVRAQTAPAQPAPTEEAKPSG
                     DASTAQEMRLEARNALVLKGESSWDDGYEKLLAAFKALRAEARRLGLATVGKPQTAFV
                     HTDDQGFRFEAMMVLDKAPDASLKPQKTFAFSLTPAGRAFVFKHAGAYDDIDSTYEAI
                     TAWLDEKGLSAKGAFVEEYLTEPEGSDDTLLELNIYVFVE"
     gene            3188..4858
                     /locus_tag="PK812_06045"
     CDS             3188..4858
                     /locus_tag="PK812_06045"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRJ69152.1"
                     /translation="MPKQHSKFRTRNEQIFARWDRLADEKFTTLGVSREALKQMQKDM
                     LGAIVLPGDPSYDQDRMLFNPVFNPLPCVIMYCESEDDVRVALNFSRQNALGFTVRSG
                     GHCTAGFSGGYGVLIDVSGLQDVVISPDGSSVTVGTGCPFKKFYAALESRGLHVPAGE
                     CDDVCVGGFVQGGGIGFTSGSFGMNCDNVESLRVMLWDGSVVVATENQNMDLWWAMRG
                     GTGGNFGILLEVTYMTYQLQFCTGWALAWSMATPQNRADCVNALLTAQRAYMVGSPYG
                     SNLTLQVLMVWQNILDPTQPPLTNKIPVFMVRGLWVGTEVDARKSMAPMLSLPGCVTQ
                     FVITDRFTKVLDNLLSNPQDQPVVDPTMGMPNEDKSSRYVATPLSTAACQEILDYFAT
                     QSPNDLTYAYLEVYSGRITAYPRQSSAFIHRDALFNTVLDVFWYLPKDRQNSETFMNG
                     WNTMIEKYWNGEVYQNYPSIAVPNYVQNYWADAAQTLSAVKMKYDPNEYFRFAQQVPA
                     TSGFDPGVGPVIPGYEAVLAAWAKPIDYSGGQPPSPGASPDGKTRQSK"
     gene            4871..8119
                     /locus_tag="PK812_06050"
     CDS             4871..8119
                     /locus_tag="PK812_06050"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRJ69153.1"
                     /translation="MSELDKVDIADWLASLGLGEYLALFVENDIDASVLAELTADDLK
                     DIGVASVGHRRKLLSAIERLARNDDTKATPAPVQADREPDTVAERRTLTVMFCDLADS
                     TALSVGMDPEDFADLIRTFRAEIVAAVQPFDAHIAQYAGDGVLAYFGYPNSSDHDAEK
                     AVEASFAIVERVRRLARFGDRSPSVRIGLATGITVVGGVERSREMRGESAVGETLNLA
                     ARLQSLAGPNGIVIAQSTRTLLGDMFVCRDLGSHALKGFADPVRAWLVEARQAAASRF
                     DALRTSAGSQPFVGRMAEMALLREARAGLGQGGARFFRIVGEAGIGKSRLVREALFVE
                     QGLRENSIILQCSPYQLSSPFYPVRYMIERLAGVSSADPEARTLEKLRAFFEQVEECT
                     QERLAVACDLLGIESDAVTGLRGLGPYQRRALTLQHLAAFAASMLRKNPVLILEDIQW
                     MDPSTAELLENVFAQVASQPVLIAATARPGPPPRWLENGSTVSIHLDRLSPEETTALV
                     RRILSDSQPFTETVRAIVERCDGIPIFAEELTRGYAAGVAEGRSEELVSNIPATLAES
                     IMARLDRLTDGRRIASTAAAIGREFPVALLTAVSGLPEKIVQSGIRELLEAGIIMPGY
                     SPFGTAVCFRQMLIRDAAYQLLLRKDRTGLHNVIVRTLRERFPAVAEDMPHIVAMQLF
                     LAGDMAASAQEWLRAGLAATRRSAYGEAVKHLRSGLDATARLPESDARDRAEIALRTT
                     LIGALIPSEGYRSPAVETEMELTLAASVRSGATESLVLGLYSKWIVLGSTGNVGAALE
                     LALKMRDIAASGSEVDRLIAWRACGTTLLFSARLHEAIEQYDLFMAHFDEARHGEALR
                     TMHGDIALMVMLGLAEAHALVANGAEAETWRGRVLEAVGRPGRAHDSVHLLVYAGCLH
                     PMLAGDMRESAQRAEELLGLVDTHNLTFWRGHALLFSGLGRIDAGDVDGGFQVAEAGI
                     ELLLKASAFNNCWYVLMARACIDHGRFDQAAASLEYARPSMDQGDLRFAPIYHWQRAR
                     LARARCEDGLFVEAHLNLARQLARAHGSRLFLEQIEAETPSLGARPASPYGT"
     gene            8387..9952
                     /gene="ffh"
                     /locus_tag="PK812_06055"
     CDS             8387..9952
                     /gene="ffh"
                     /locus_tag="PK812_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003601551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HRJ69154.1"
                     /translation="MFEGLSGRLSGILDKLRGRGALSEADVGEAMREVRRALLEADVA
                     LDVVRSFTDRVRNRAVGAEVIKSVTPGQMVVKIVHDTLVETLGADAQPIDLNSVPPVP
                     VLMVGLQGSGKTTTTAKIALRLTDKAKRRVLMASLDTRRPAAMEQLAVLGKQIGVETL
                     PIVAGQTAVQIARRALDAARLGGFDVVMLDTAGRTTVDEALMAEVAEVKAATNPHEVL
                     LVVDALTGQDAVNTAKAFESRVGVTGIVLTRVDGDGRGGAALSMRAVTGKPIKLIGVG
                     EKMDALEDFHPARIANRILGMGDIVSLVEKAAETIDKDQALRIADKMRKGKFDLDDMR
                     AQLQQMEKLGGMGGLMGMLPGVAKIKDQIAQANLDETMFKRQVAIINSMTPWERRNPD
                     LLKHSRKKRIAAGSGTDAAEINKLLKMHRGMADMMKSMAAGPKRGAMAGLANMFGLGG
                     GMPQPTPEQLAALEKQMGGKLPDMPAELTKGIPAIPPGTLPGLGKPGLPGLGGLGKLP
                     GLGGFNPFGGKKK"
     gene            10089..10439
                     /gene="rpsP"
                     /locus_tag="PK812_06060"
     CDS             10089..10439
                     /gene="rpsP"
                     /locus_tag="PK812_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HRJ69155.1"
                     /translation="MSVKIRLSRGGAKKRPYYYIVIANSTAPRDGRFIEEVGAYNPTL
                     PKDSAERVKLDVEKIKGWLAKGAQPTDRVARFLDAAGLMKRSPRNNPQKAVPGKKAQE
                     RADAKAKKAESPAA"
     gene            10537..11133
                     /gene="rimM"
                     /locus_tag="PK812_06065"
     CDS             10537..11133
                     /gene="rimM"
                     /locus_tag="PK812_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183305.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HRJ69156.1"
                     /translation="MVRRKRPLSDTPRAAPAAPETRAPEGPMVQMGVFGAAVGLRGEI
                     RVKSWTGDPEAIGDYGPLFAADGRRFDVEVLRLQKDMAIARVKGVSDRTAAEKLTNVE
                     LFVPRAALGEVADEDEFFHADLVGLRAETADGTLIGTVTAILNFGAGDVVEVRPPRGP
                     SRAFPFTKAVVPVVDIPGGRIVVVPPTEVEGDEGRGPV"
     gene            11133..>11361
                     /locus_tag="PK812_06070"
     CDS             11133..>11361
                     /locus_tag="PK812_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRJ69157.1"
                     /translation="MFCATVLTLYPEMFPGPLGLSLAGEGLRGGLWALEARNIRDFGH
                     GRHRAVDDTPAGGGPGMVLRADVLAAAIDAAA"
BASE COUNT         1898 a         3674 c         3850 g         1939 t
ORIGIN      
        1 gccggaaccg caggcgaggg tcaagccggc gccgcgctcc cataccttct ggatcagatg
       61 gccgggcgcg gcgaccgcca cgagagagat gttggcgcgc tcgggaaaga ccgggtcggc
      121 ctcgagccgc ggcccggtgc gcgcgaggtc atgcgcctcg acatcgtcga cgaagaagac
      181 ggcgtgcgga ttgcccatgt tgaccgcgcc gaaccaggcc ggaaggccgg ccttcagggc
      241 cgtcgcgtca tggggcacgc gcgccgtgtc ggcgacaggg tgcgctagcg ggatctcccg
      301 ccagccgagg cgtggcgcac ccatgtcgac ggtgatgcgc tcgggcgatc cggaggcgcg
      361 caggacgccg gccagagtct cgatggtcgc ggcatcgcgc tccccttcgc gcatcagcac
      421 ctgggcgacg cagcgggtgg cgttgccgca ggcgccggac tgagagccgt cggggttgta
      481 gatgcgcatg aagacgtcgg cgcgccgcga gggttcgagc acgatcagct ggtcgcaccc
      541 gacgccgtag cggcggtcgg cgatggcccg ggccagatcg ggcgtgaccg ggattggcgt
      601 gtcgcggccg tcgagcacga cgaagtcatt gccgaggcca tgcatcttaa cgaagtcgag
      661 catttgtgcg cgaggtttcc gctggttgtg gtcccttttc gcagaaaggg cttgcaaatc
      721 ccacctaatg gggttcatcc ctctctcaaa cgtctggact tggcaagcgc ggagcgcgcc
      781 ggcgcgaggg gaagcggatg cgattggtca tcctggtcct gtcgatcggg attggaacct
      841 cggctgtccc ggccgaggaa agcggcctcg taacccccat tcaggtgcct catctgccgc
      901 tcggactggt gaccttgcag aagacccggg cgatcaacct ggcggtgtcg cccggcgccg
      961 gtgcgttccg gctggccagt gatccgcccg ggcgcatctg gacggtgacc gatcgcggac
     1021 ccgtcgtcga ttgcagcgag gaacgcgaga ccatcgggcc cgacgacaag gcgatctgcg
     1081 caccggaccg gcgcggcaag atctacctgc tgccgggata cgtgccctcg atctacgcgc
     1141 tggatgtcaa cggcgacggc acggcgcggt tcgccgagat gatcccgctg cggggcaaga
     1201 gcggcaagct gctgtcgggc ctgaccaacc cgctcggccc gcagcgcact gaaacgggct
     1261 acacgaccga ggggcgcgtg ctcgacaccg atccttccgg catcgagccg ggaggcctcg
     1321 tccggctggc ggacgggacc ttctggatcg cggagaaatt cggtccgtcg ctgcttgagg
     1381 tggcccgcga tgggcaggtg ctgcgccggc tggtgccggc caatcttgaa cccgagctga
     1441 aaggcgcgga ctatgcggtc gagccggctt ttccgtcgat cctgagcctg cgcgcggtgg
     1501 cgcgcggatt cgatgccatc gccatctccc cggacgagcg gtttctttat gtggcgatgc
     1561 agaacgccct ggcgaacccg gccttggaag cgtaccggca ctcgccggcg acccggatct
     1621 ggaagattga gcgcacgagc ggccggatcg tcgcggaata cgtctatcgg cttgatccgc
     1681 ccggcaattt tccggccgac accagtgttg ccgggcgtcc gctgcgccag agcgacgtgc
     1741 gcattgccga aatcgtcgcg atgggggaga accggctgct ggtgctggaa cgggtgcgcc
     1801 acggctcgcg catctttgtc gcgaccctga gccctggcca ggaattgccg ccgccctacg
     1861 atgatgaaaa gacccgcccc tcgctggaag agctctttcc cgatgtctgg gagatcgccg
     1921 gaatcgcgcc ggtgaagaag atgttgctgg tggattcgga acggatcaag gggatcggcg
     1981 gccggctgac ggcgatggcg gtgctctcgc cgcgtgaact ggtgctgatg tcgaacaatg
     2041 tctacggtgt cgaaggcggt cgcagccaga cgtttcgctt gaccttttcc gcgcccgtct
     2101 ttcagtgagc ggcggcgaat cggacggtcc cgcgcgccgg cgcggtccgt tcatggttct
     2161 gccgcagatc cgtgcgttca tgatctgctc agcaggaccg gagtgaattg gcgtcttgag
     2221 gctttccagg cttggagcgg gaaggaatga ccacaggtca tcaggcgttc ggacggatct
     2281 gcgccatcat gctggccggc gtgctgctcg ccgtcccggt gcgtgcgcag accgcgcccg
     2341 cccaaccggc gccgaccgag gaggcaaagc cctcgggcga tgcctcgacc gcccaggaga
     2401 tgcgccttga agcccgcaat gccttggttc tcaagggaga atcgagctgg gacgacggct
     2461 atgaaaagct gctcgccgcc ttcaaggcgc tgcgcgctga ggcccgccgc ctcggcctgg
     2521 ccacggtcgg caagccgcag accgccttcg tccataccga cgaccagggg ttccgcttcg
     2581 aggctatgat ggttctcgac aaggccccgg acgccagcct caagcctcag aagacatttg
     2641 ctttttccct tacgccagcg ggtcgggcct tcgtcttcaa gcacgccggc gcctatgacg
     2701 acatcgacag cacctacgag gcgatcacgg cctggctgga tgaaaagggc ctttcggcca
     2761 agggtgcttt cgtcgaggaa tacctgaccg agccggaagg ctccgacgac acgctgctgg
     2821 aactcaacat ctacgttttc gtggaatagg cgggggcttc ccatcgctgg cgaagccggg
     2881 atgcttctcc cgagatggtc tggcaatgtc gggaggggtt ccggccgcag ccaggtgatc
     2941 gaatcggtcc ccggcgcgcc atcccgcatt cggggtggat gcgaaggcgc cagcggcagg
     3001 gacggtgatc aggtccgggc acccggacgc ccctgcctga aggcggttcg ggcaccttgt
     3061 gcggaaataa cacgccagtc gcccttattg tcattgtcag gcgcatcgcg ccttcgtacc
     3121 tttcccttgc gtcgccatgc ggagttgcgc agctgctgcg gcgcgcggct gtgccccggg
     3181 ggaaacaatg ccgaaacagc attcgaagtt ccgcacgcgc aatgagcaga ttttcgcgcg
     3241 ctgggaccgt ctagcggatg aaaaattcac gacgctcggt gtcagccggg aagcgctgaa
     3301 gcaaatgcag aaggacatgc tcggcgccat cgtcctgccg ggcgatccca gctacgacca
     3361 ggaccggatg ttgttcaatc cggtattcaa cccgctaccc tgcgtcatca tgtattgcga
     3421 gagcgaagac gatgtgcgtg tggctctcaa cttttcccgc cagaacgccc tcggcttcac
     3481 cgttcgctcc ggcgggcatt gcacggccgg cttttccggc ggctacgggg tgctgatcga
     3541 tgtcagcggg ttgcaggacg tcgtgatctc gccggacggg agctccgtga cggtgggtac
     3601 cggctgcccg ttcaagaaat tctacgcggc gctcgaatcc cgcggcctgc atgtgcctgc
     3661 cggtgagtgc gatgacgtct gcgttggcgg ctttgtgcag ggcggcggca tcggcttcac
     3721 ctccggttcc tttggcatga actgcgataa tgtcgagagc ctgcgcgtga tgctctggga
     3781 cggttcggtc gtcgtcgcga ccgaaaacca gaacatggac ctctggtggg ccatgcgggg
     3841 tggaaccggc ggcaatttcg gcatcctgct cgaagtgacc tacatgacgt atcaactgca
     3901 gttttgcacc ggatgggctt tggcctggtc catggcgaca ccgcagaacc gcgccgattg
     3961 cgtcaatgcg ctgctgacgg cccaaagggc ctacatggtt ggcagcccct acgggagcaa
     4021 tctgacgctg caggtgctga tggtctggca gaatattctc gatccgacgc agccgcccct
     4081 cacaaacaag atcccggtct tcatggtgcg cggcctctgg gtcggaacgg aagtggacgc
     4141 gcgtaagtcc atggcgccca tgctctccct acccggctgc gtcacccagt tcgtcatcac
     4201 agaccgcttc accaaggtgc tcgacaacct gctgtcgaat ccccaggatc agcccgtggt
     4261 cgatcccacg atgggcatgc ccaatgagga caagtcctcg cgctatgtcg cgacgcccct
     4321 gtccaccgcg gcatgccagg aaattctcga ttatttcgcc acccaaagtc ccaacgatct
     4381 gacctacgcc tatcttgagg tgtacagcgg tcgcatcacc gcctatccac gccagagcag
     4441 cgccttcatt caccgcgatg cgctgttcaa caccgttctc gatgttttct ggtatctgcc
     4501 caaggatcgg cagaattccg agactttcat gaatggctgg aacacgatga ttgagaaata
     4561 ctggaacggc gaggtctatc agaactatcc aagcatcgcg gttcccaatt acgtccagaa
     4621 ttactgggcc gatgccgctc agacgctctc ggcggtcaag atgaaatacg acccgaacga
     4681 gtacttccgg tttgcgcagc aggtgccagc gacctccggt ttcgatcctg gggtcggccc
     4741 ggtcattccc ggatatgaag cggtcctggc tgcctgggcc aagccgatcg actattcggg
     4801 tggacagccg ccgtcgccag gggccagtcc ggacggcaag acgcgtcaat cgaaatgagt
     4861 cgctgatttc gtgtccgaac tcgacaaagt tgacatcgca gactggctcg caagcctcgg
     4921 ccttggcgag taccttgcct tgttcgtcga gaatgacatc gacgcctcgg tcctggctga
     4981 actgaccgca gacgacctga aggacatcgg cgtggcgtcg gtcggccaca ggcggaagct
     5041 gctgtcggcg atcgaacgtc tggcgcggaa cgacgacacg aaggcgacgc cggcgccggt
     5101 gcaggccgat cgcgagccgg acaccgtcgc cgagcgccgg acgctgacgg tgatgttctg
     5161 cgatcttgcc gattccacgg ccctgtctgt cggcatggat ccggaagatt tcgccgacct
     5221 catccgcacg tttcgcgccg agatcgtggc ggcggtgcaa ccgttcgacg cgcatatcgc
     5281 acagtatgcc ggcgatgggg tgctggccta tttcgggtat ccgaattcct ccgatcacga
     5341 cgccgaaaag gcggtggaag ccagtttcgc catcgtcgaa cgcgtgcgtc gcctggcccg
     5401 tttcggtgac cgcagcccca gcgtccgcat cggccttgcg accggcatca cggttgtcgg
     5461 cggtgtcgag cggtcgcggg aaatgcgggg cgaaagcgcg gtgggcgaaa ccttgaacct
     5521 cgccgcccgg ctccaatccc tggcggggcc caatggaatc gtcattgcgc agtcgacccg
     5581 gactttgctc ggagacatgt tcgtctgccg cgatcttggc tcgcatgcgc tcaagggctt
     5641 cgcagatccg gtccgggcct ggctggtgga ggcgcggcag gccgcggcca gccggtttga
     5701 cgccctgcgc acctcggctg gatcgcaacc cttcgtcggg cgcatggcgg aaatggctct
     5761 tctgcgggag gcgcgggccg ggctggggca gggcggcgcg cggtttttcc gtatcgtcgg
     5821 cgaggcgggc atcggcaagt cgcggctggt gcgcgaggcg ctgttcgtcg agcagggcct
     5881 gcgcgagaac agcatcatcc tgcaatgctc tccctaccag ttgagcagcc cgttctatcc
     5941 cgttcgttac atgatcgagc gcctggccgg cgtgtcgtca gccgatccgg aagcccggac
     6001 gctcgaaaag ctgcgcgcct tctttgaaca ggtcgaggag tgcacgcagg agcgccttgc
     6061 ggtcgcctgt gatctgctgg ggatcgagtc ggacgcggtc acgggcctgc gcgggcttgg
     6121 tccctatcag cgacgcgccc tcacgttgca gcaccttgcc gccttcgccg cctccatgct
     6181 gcgaaagaat ccggttctca tcctcgagga tatccagtgg atggacccct cgacggctga
     6241 acttctggaa aacgtcttcg cgcaggtcgc gagccagccg gtcttgatcg ccgccaccgc
     6301 ccgccccggg ccaccgccgc gctggctgga aaatggctcg accgtttcca tccatctcga
     6361 ccgcctgtcg ccggaagaaa cgacggcgct tgtccgccgc atactgtcgg actcgcagcc
     6421 cttcaccgag acggtgcgcg ccattgtcga gcggtgcgat ggcattccga tcttcgccga
     6481 ggaactgacg cgcggctatg ccgccggtgt ggcggagggg cggtcagagg aactcgtttc
     6541 caacattcca gcgacactcg ccgaatcgat catggcccgc cttgaccggc tcacggacgg
     6601 acgccgaatt gcctcgacgg cggcggccat cggtcgtgag ttccccgtcg ccctgctgac
     6661 ggccgtctcg gggctgccgg aaaagatcgt gcagtccggc atccgggaac tgctggaagc
     6721 cggcatcatc atgccgggct acagtccgtt cggcacggcg gtctgctttc gtcagatgct
     6781 gatccgcgat gcggcttacc agctgctgct gcgcaaggac cggaccggcc tgcacaacgt
     6841 catcgtccgg acgctgcgcg agcggttccc tgcggtggcg gaggacatgc cgcatatcgt
     6901 cgcgatgcag ctctttctcg cgggcgacat ggcggcctcg gcgcaggaat ggctgcgcgc
     6961 cggacttgct gcgacgcggc gctccgccta tggcgaagcc gtcaagcact tgcgcagcgg
     7021 acttgatgcg accgcccgcc tgcccgaatc cgacgcgcgc gatcgcgcgg aaatcgccct
     7081 tcggaccacg ctgatcggtg ccctcatccc aagcgagggc taccgctccc cggcagtcga
     7141 aacggaaatg gagttgacgc tcgccgccag cgtccggtcc ggcgcgacgg aaagcctggt
     7201 tctggggctc tattcgaaat ggatcgttct cggcagcacc ggcaacgttg gcgccgcgct
     7261 ggaactcgcg ttgaagatgc gcgacatcgc cgcgtccggc agcgaggtgg accggctgat
     7321 cgcatggcgg gcctgcggca cgacgctgct gttcagcgcg cggctgcacg aggcgatcga
     7381 gcagtacgac ctgttcatgg cccacttcga cgaggcacgc catggcgagg cgttgcgaac
     7441 catgcatggc gatatcgccc tgatggtgat gctcggcctc gccgaagcgc atgcgctcgt
     7501 ggcgaacggc gcagaagcgg agacatggcg ggggcgggtt ctggaggcgg tgggccgtcc
     7561 agggcgggcg catgacagtg tgcaccttct ggtctacgcc ggctgcctgc atccgatgct
     7621 cgccggagac atgcgcgaat ccgcgcagcg cgccgaggaa cttctggggt tggtcgatac
     7681 gcacaatctc acgttctggc gcggccacgc cctgctgttc agcggacttg gccgtatcga
     7741 cgcgggcgat gtcgatggcg gattccaggt cgccgaagcc ggcatcgagc tgctgctgaa
     7801 ggccagcgcc ttcaacaatt gctggtatgt tctcatggcc agggcctgca tcgatcatgg
     7861 acggttcgac caggcagcgg cgtcgctcga gtatgcgcgt ccctcgatgg atcagggcga
     7921 tctgcgcttc gctccgatct accattggca gcgcgcccgc ctcgcgcgcg cccgctgcga
     7981 ggacggcctg tttgtcgagg cgcatctgaa tcttgcccgc cagctggcgc gcgcgcatgg
     8041 atcgcggctg tttctggagc agatcgaggc ggagacgccg tcgctcggcg cgcgcccggc
     8101 aagcccttac ggaacctgag cgttttcctt cgacccggcg cctgcgcggc ggcctgggaa
     8161 cgcctgccgg ttgacgtttc ggccactccg gcgtaagagc gcggccaatt ccgatctggc
     8221 cgatctttcc gatccgccga ccgggcctga gcgcccggag gtcaacgccc gacagcgcga
     8281 tgcgccctcg ggcgcgaaac cgtttggaag tccggggcgc aagtcccgtc ggtgaaatcc
     8341 tcgtagaacc cgcagtgctc gggtttgaaa gccaggagaa cggcgcatgt tcgaaggttt
     8401 gtccggaagg ctgtccggca tcctcgacaa gctgagggga cgcggcgccc tgagcgaggc
     8461 cgacgtcggc gaagccatgc gcgaggtgcg ccgcgcgttg ctcgaggcag acgtggcgct
     8521 cgatgtcgtg cgctccttca ccgaccgggt gcggaaccgc gccgtcggcg ccgaggtgat
     8581 caagtcggtc accccgggcc agatggtggt gaagatcgtc cacgacacgc tcgtggagac
     8641 gctgggcgcc gatgcccagc cgatcgacct gaattccgtg ccgccggtgc cggtgctgat
     8701 ggtcggcctg cagggttcgg gcaagaccac gaccaccgcc aagatcgcgc tgcggctgac
     8761 cgacaaggcc aagcgccgcg tgctgatggc ctcgctcgac acccgccgcc cggcggcgat
     8821 ggagcagctt gccgtgctcg gcaagcagat cggcgtcgag acgctgccga tcgtcgccgg
     8881 ccagaccgcg gtgcagatcg cccgccgcgc gctggacgcc gcccggctcg gtggcttcga
     8941 cgtggtgatg ctggataccg ccggccgcac cacggtcgac gaggcgctga tggcggaggt
     9001 ggccgaggtc aaggccgcca ccaacccgca tgaggtgctg ctggtcgtcg acgcgctgac
     9061 cggacaggac gcggtgaaca cggcgaaggc cttcgaatcc cgcgtcggcg tcaccggcat
     9121 cgtgctgacc cgcgtcgacg gcgacgggcg cggcggcgcg gcattgtcga tgcgcgccgt
     9181 caccggcaag ccgatcaagc tgatcggcgt cggcgagaag atggatgcgc tggaggattt
     9241 tcatcccgcc cgcatcgcca atcgcatcct cggcatgggc gacatcgtct cgctggtcga
     9301 gaaggcggcc gagacgatcg acaaggacca ggcgctgcgc atcgccgaca agatgcgcaa
     9361 gggcaagttc gacctcgacg acatgcgcgc ccagctccag cagatggaaa agctcggcgg
     9421 catgggcggg ctgatgggca tgttgcccgg cgtcgccaag atcaaggacc agatcgcgca
     9481 ggcgaacctc gatgagacga tgttcaagcg ccaggttgcg atcatcaatt cgatgacgcc
     9541 gtgggagcgc cgcaacccgg acctcctgaa gcacagccgc aagaagcgca tcgccgccgg
     9601 ttccggcacg gacgcggccg agatcaacaa gctgctgaag atgcatcgcg gcatggccga
     9661 catgatgaag tcgatggcgg cgggcccgaa gcgcggcgcc atggcggggc ttgccaacat
     9721 gttcggcctt ggcggcggca tgccgcagcc gacgccggaa cagctcgccg cgctcgagaa
     9781 gcagatgggc ggcaagctcc ccgacatgcc ggcggagctg acgaagggca ttcccgcgat
     9841 tccgcccggc acccttccgg gcctcggcaa gccgggtctt cccggtctcg gcggcctcgg
     9901 caagctgccc ggcctcggcg gcttcaatcc gttcgggggg aagaagaagt gaggtttcct
     9961 ccgtcattgc gagcgaatcg aaacacgccg gacgtcacga acacgccggc ctgtttcgtc
    10021 gccatcggct cctcgcgatg acgatcacaa tcaccgcatt catcaaggac tgaacaggag
    10081 taaccgaaat gtccgtgaag atccgcctct cccgtggtgg cgccaagaag cgcccctact
    10141 actacatcgt cattgccaac tcgacggcgc cgcgcgacgg ccgcttcatc gaggaagtcg
    10201 gcgcctacaa cccgaccctg ccgaaggact cggccgagcg cgtgaagctc gacgtggaga
    10261 agatcaaggg ctggctcgcc aagggcgcgc agccgaccga tcgcgtcgcc cgcttcctcg
    10321 acgccgccgg cctgatgaag cgcagcccgc gcaacaaccc gcagaaggcg gttccgggca
    10381 agaaggccca ggagcgcgcc gacgccaagg ccaagaaggc ggaaagcccg gcggcgtaag
    10441 cccgcggcgt ccttgcgagg tgggttcacg cccggcgatg cgctccagac gcagatgctg
    10501 atggcttcgg acgcctcgca atgacggaca agccccatgg tccggcgcaa gcgccccctc
    10561 tccgacaccc cacgcgcggc cccggccgcg ccggaaaccc gcgcgccgga aggccccatg
    10621 gtgcagatgg gcgtgttcgg tgcggcggtg gggctgcgcg gcgagatccg cgtcaagtcc
    10681 tggaccggtg atcccgaggc gatcggcgac tacggtccgc tgttcgcggc cgacgggcgg
    10741 cgcttcgacg tcgaggtcct gcggctgcag aaggacatgg cgattgcgcg cgtgaagggc
    10801 gtttccgacc gcacggcggc ggaaaaactc accaatgtcg agcttttcgt gccgcgcgcc
    10861 gcgctcggcg aggtcgcgga cgaggacgag ttcttccatg ccgatctcgt cggcctcagg
    10921 gccgagacag cggacggaac cctgatcggc acggtgacgg cgatcctgaa tttcggcgcg
    10981 ggcgacgtgg tggaggtgcg ccccccgcgc ggtccttccc gtgcctttcc gttcacgaag
    11041 gccgtcgtgc cggtggtcga catcccgggc gggcgcatcg tcgtggttcc gccgacggag
    11101 gtcgaggggg atgagggtcg cgggcccgtg tgatgttctg cgcgaccgtc ctcaccctct
    11161 atcccgagat gtttccaggt cccctcggcc tgtcgctggc gggggagggc ctgcggggcg
    11221 ggctatgggc gcttgaagct cgcaacatcc gcgatttcgg gcacggaagg caccgcgccg
    11281 tagacgatac cccggccggt ggcgggccgg gcatggtgct gcgcgccgac gtgctggcgg
    11341 ctgcgatcga cgcggccgcg c
//
LOCUS       DAOMXN010000056        11333 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_3082_length_11333_cov_10.497429, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000056 DAOMXN010000000
VERSION     DAOMXN010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 11333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11333
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3082_length_11333_cov_10.497429"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..1459)
                     /locus_tag="PK812_06075"
     CDS             complement(53..1459)
                     /locus_tag="PK812_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HRJ69158.1"
                     /translation="MTIRLPLAAAFVFLLAAAPAKAQLFQRETPQSRQEITLSFAPVV
                     KKAGPAVVNVYGARRQQQTRHPLFDDPFFREFFGGGRAPRERMQQSVGSGVLVASDGI
                     VVTNHHVIEGMSEVKVALPDKREFEAEVLLRDPKTDLAVLKMKSTEKFPLVELGNSDE
                     LQVGDLVLAIGNPFGVGQTVTQGIVSALARTQVGVSDYQFFIQTDAAINPGNSGGAMV
                     DMQGRLVGINTAIYSRSGGSHGIGFAIPASMVRVVVDSARSGGKTVRRPWFGARMQSL
                     TAEIAESMGLDRPIGALIASVAKGSPAAQAGVQAGDVVVAVDGATVEDPDGFGFRFAT
                     KALGGTSSITVLRSGKRVVLPVKLQSAPETTPRDQRTIAGRSPFSGLVVVNLSPAAAD
                     ELSLSADAEGVAVADVTPGSPAQQVGFQKGDVLLAINNEPAKTTRDVERLTAARSGFG
                     WRVTVSREGQTLTIILGG"
     gene            1635..2639
                     /locus_tag="PK812_06080"
     CDS             1635..2639
                     /locus_tag="PK812_06080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HRJ69159.1"
                     /translation="MLNRILTAGVATAALLALSSAPALAQAKTIAIDGSSTVFPVMEA
                     IAEEFQKSKKGQVRVTVGISGTGGGFKKFCRGETDASNGSRPILKAEIEECKKNGVEF
                     MELPIAFDALTVAVSPKNTFLQCVTVEQLKKIWEPSAQGKMMKWKDVDPSWPDQPMKL
                     FGAGSDSGTFDYFTEATVGKAKASRGDFTASEDDNVLVQGIETDANSLGYIPFAYYAP
                     HAAKMKALAIDWGKGQGCVKPSLENVLAGTYNPLSRPLFIYISKKSADKPEVKEFVEF
                     AMKNAVPLIKEVKYLPLPDRAYPAALQRFQKGVTGSAFGGVPEVGVSIDELLKREPKV
                     "
     gene            2723..3631
                     /gene="pstC"
                     /locus_tag="PK812_06085"
     CDS             2723..3631
                     /gene="pstC"
                     /locus_tag="PK812_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HRJ69160.1"
                     /translation="MATRRTPQELRKMRARERAIEWALFAAAASSVAVTIAIVAVLLY
                     ESLGFFAHVPLGTFLTDTMWTPLFADPRYGVLPLLGGTLLSTFVAMLVAGPLGLTAAI
                     YLSEFASSRVREVVKPTLELLSGVPTVVYGFFALLFVTPALQAIIPGLPGFNVLSAGI
                     VMGIMIIPYIASLSEDAMRAVPNGLRDGSLALGATRLETALRVVLPSAFSGVASAFVL
                     GISRAVGETMVVAIAAGQQPNLTIDPREPAATITAYIVQVALGDLPHGSVGYQTIFAV
                     GLVLLLMTLFFNVAAFWLRAKFREAY"
     gene            3631..4578
                     /gene="pstA"
                     /locus_tag="PK812_06090"
     CDS             3631..4578
                     /gene="pstA"
                     /locus_tag="PK812_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HRJ69161.1"
                     /translation="MSRADTVTVAAPAKAALGKGIPARRRREKIFNAIGIGSTLVGLI
                     ALSLLLFDLAYHGLPRISWDFFVNFPSRRATQAGILSAWVGSLLIIVVTAVFGVTLGV
                     SSGIYLEEYAKRNWVTNIIEVNVNNLAGVPSIVFGLLALGLFVYGLGTGRSILTAGLT
                     LGLLILPIIIVGTREAIRAVPQSLREAAYGVGATKWQVTRDHVLPAATPGILTGVIIG
                     ISRALGETAPLITIGALTFIAFLPTSPVSDTFPFISFQWLFDGFTVLPIQAFNWISRP
                     GHDFHANAAAAGLVLLAITLSLNAIAIILRYRLRKKLQW"
     gene            4593..5405
                     /gene="pstB"
                     /locus_tag="PK812_06095"
     CDS             4593..5405
                     /gene="pstB"
                     /locus_tag="PK812_06095"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HRJ69162.1"
                     /translation="MTTTDIAATTVSEGSVAEAPIKIDIDKVSVFYGEKQAIKSVSTR
                     IPARAVTSFIGPSGCGKTTLLRSINRMNDTIPGCRVTGRLAIDGQDIYAPSIDVVELR
                     SRVGMVFQKPNPFAKSIYENVAYGPRIHGLARSKTEMDEIVESSLRRASLWDEVKDRL
                     NQPGSGISGGQQQRLCIARAIAVSPDIILMDEPCSALDPIATAKIEELIDELRVNYTI
                     VIVTHSMQQAARVSQRTVFFHLGDIVEDGPTTEIFTSPREKRTQDYITGRFG"
     gene            5423..6136
                     /gene="phoU"
                     /locus_tag="PK812_06100"
     CDS             5423..6136
                     /gene="phoU"
                     /locus_tag="PK812_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HRJ69163.1"
                     /translation="MAEHTVKSFDQELKDLAARISEMGGIAESMLSRAVESLVKGDTH
                     LAEVVIQADLRLDALEREVADKAVLVIARRQPMADDLRFIVSSIKLAADIERIGDLAK
                     NIGKRVKAMGHPFLQKSLLSGITHLGDLTLERLHQVLDAYANSDVAAALDVWQRDDDI
                     DALHNSLFRELLTYMMEDPRSISVCAHLLFCAKNIERMGDHSTNIAEIIHYMASGKPL
                     LQDRPRQGAVPDSDAPAGK"
     gene            complement(6241..6675)
                     /gene="rplQ"
                     /locus_tag="PK812_06105"
     CDS             complement(6241..6675)
                     /gene="rplQ"
                     /locus_tag="PK812_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009463031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HRJ69164.1"
                     /translation="MRHGNKGRRFNRMASHRKAMFANLAQALITHEQIVTTLPKAKDL
                     RPVVEKLVTLGKRGDLHARRQAIAQIRDADVVKKLFDVLGPRYKERNGGYTRVLKAGF
                     RYGDNAPLAVIEFVDRDVDAKGKADLERHAAQQKEAPAEAAA"
     gene            complement(6783..7799)
                     /locus_tag="PK812_06110"
     CDS             complement(6783..7799)
                     /locus_tag="PK812_06110"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HRJ69165.1"
                     /translation="MIQKNWQELIKPNKLEVSPGADPRRLATVVAEPLERGFGMTLGN
                     ALRRVLLSSLQGAAVTSIQIDGVLHEFSSIPGVREDVIDIVLNVKDIAIKMQSDQPKR
                     LTLRKHGPGAVTAGDIQMTGDAQVLNPELVICTLDEGAEIRMELTVASGKGYVPADRN
                     RPEDAPIGLIPVDSLFSPVKKVSYRVENTRSGQILDYDKLTMSVETDGSVTPEDALAY
                     AARILQDQLEVFLNFEEPRKEEAPAAIPELAFNPALLKKVDELELSVRSANCLKNDNI
                     VYIGDLIQKTEAEMLRTPNFGRKSLNEIKEVLAQMGLHLGMEVTGWPPENIEELAKRF
                     EEHY"
     gene            complement(7876..8265)
                     /gene="rpsK"
                     /locus_tag="PK812_06115"
     CDS             complement(7876..8265)
                     /gene="rpsK"
                     /locus_tag="PK812_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HRJ69166.1"
                     /translation="MAKEATRVRRRERKNIASGVAHVNASFNNTMITITDAQGNTISW
                     SSAGALGFKGSKKSTPYAAQVAAEDAARKASEHGMRTIEVEVCGPGSGRESALRALQA
                     SGFTVTSIRDVTPIAHNGCRPRKRRRV"
     gene            complement(8330..8698)
                     /gene="rpsM"
                     /locus_tag="PK812_06120"
     CDS             complement(8330..8698)
                     /gene="rpsM"
                     /locus_tag="PK812_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011651425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HRJ69167.1"
                     /translation="MARIAGVNIPTQKRVVIALRYIHGIGPVNAVEICDKVGIPHDKR
                     VNQLTDAEVLQIRETIDRDYTVEGDLRRDTAMNIKRLMDLGCYRGLRHRRGLPVRGQR
                     THTNARTRKGKPKAIAGKKK"
     gene            complement(8986..9570)
                     /locus_tag="PK812_06125"
     CDS             complement(8986..9570)
                     /locus_tag="PK812_06125"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HRJ69168.1"
                     /translation="MRLILLGPPGAGKGTQAQRIVEKYGIPQLSTGDMLRAAVAAGSP
                     VGLKAKAVMESGGLVSDEIVVGIVEERIGQSDAQKGFILDGFPRTVAQAEALDAMLSR
                     KGVKLSGCVELVVDETKLVDRIVKRAEDARKAGQPVRKDDDPEVFKTRLAAYHRDTAA
                     VTPYYRAKGMLKTVDGMQPIDGVTAALMQVLAAL"
     gene            complement(9567..10898)
                     /gene="secY"
                     /locus_tag="PK812_06130"
     CDS             complement(9567..10898)
                     /gene="secY"
                     /locus_tag="PK812_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HRJ69169.1"
                     /translation="MASAAEQFAANLNFGAFAKAEELKKRIWFTLGALLVYRLGTYIP
                     LPGINPEAVAEIFKTQMSGVLGLFNMFSGGAVGRLAIFALNIMPYISASIIIQLLTTV
                     SPQLEALKKEGESGRRQLNQYTRYLTVVLALFQAYGISVGLEASGNVVRDPGLFFRLS
                     TVITLTGGTLFMMWLGEQITSRGIGNGTSLIIFAGIVAELPSAIAGTLELSRQGALST
                     GLILLTMVMGIAVIALIVFVERAQRRLLLNYPKRQVGNRVYEGQTSFLPLKLNTSGVI
                     PPIFASSLLLLPTTVTGFAQSGGPEWLQTMNAYLGHGRPLFMILYAALIIFFCFFYTA
                     IVFNPVETADNLKKQGGFIPGIRPGERTAQYIDHVLSRITVVGALYLTLICLLPELLV
                     SQSGLPFYFGGTSILIVVSVTMDTVAQVHGHLLAHQYEGLIKRNKLKGKRK"
     gene            complement(11013..>11333)
                     /locus_tag="PK812_06135"
     CDS             complement(11013..>11333)
                     /locus_tag="PK812_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL15m family ribosomal protein"
                     /protein_id="HRJ69170.1"
                     /translation="LHRRLPKRGFTNIFAKDLNEVNLGRVQQAVDAGKLDAKAPVTEA
                     ALVAAGVCARARDGVKILGQGELKAKLAFEVYGASKSALAAIEKAGGSVKLLAPAKEA
                     PAQA"
BASE COUNT         2009 a         3829 c         3449 g         2046 t
ORIGIN      
        1 ctccactttc tacctgtata cttaatccca tgagccgccg catcccgccc catcagccgc
       61 cgagaatgat ggtgagcgtc tgcccttccc gactgaccgt gacacgccag ccgaacccgc
      121 tgcgggcagc cgtcagccgt tccacgtcgc gtgtcgtctt cgccggctcg ttgttgatgg
      181 cgagcagcac atcgcccttc tggaacccga cttgttgcgc cggcgagcct ggggtcacat
      241 ccgccacggc gacgccctcg gcgtcggcgc ttagcgacag ttcgtccgcc gccgcgggcg
      301 acaggttgac gacgaccagc ccggagaacg gcgatcgacc ggcgatggtg cgctggtcgc
      361 gcggcgtggt ttccggtgcc gactggagct tcacgggaag cacgacgcgc ttgcccgacc
      421 gcagcaccgt gatggaactc gtgccgccca gcgccttcgt cgcgaagcgg aagccgaagc
      481 catccggatc ttccaccgtc gcgccatcga ccgccacgac cacatcgccg gcctgcaccc
      541 ccgcctgcgc cgccgggctc cccttggcga ccgaggcgat cagggcgccg atcggccggt
      601 cgagacccat gctctcggcg atctccgccg tcagactctg catgcgggcg ccgaaccacg
      661 ggcgccggac ggtcttgccg ccgctgcggg cactgtcgac cacgacgcgg accattgatg
      721 cggggatggc gaagccaatg ccatggctgc cgcccgatcg cgagtagatc gccgtgttga
      781 tgccgaccag gcggccctgc atgtccacca tcgcgccgcc ggaattgccc ggattgatgg
      841 ccgcgtcggt ctggatgaag aactggtagt ccgaaacgcc gacctgcgtg cgcgccagcg
      901 cggatacgat gccctgggtc acggtctggc caacaccgaa cggattgccg atggcgagca
      961 cgagatcgcc cacctgcaac tcgtccgaat tgcccagctc caccagcgga aacttctccg
     1021 tgctcttcat tttcagcacg gcgaggtcgg tcttggggtc gcgcaacagg acctccgcct
     1081 cgaactcccg cttgtcgggc agcgctacct tcacctcgct catgccctcg atgacgtgat
     1141 ggttggtgac gacgatgcca tccgacgcga ccagcacgcc ggagccgact gattgctgca
     1201 tgcgctcgcg cggcgcacgc cctccgccga agaactcccg gaagaaagga tcatcgaaca
     1261 gcggatgcct cgtctgctgt tggcgccgcg caccgtagac gttgaccacc gccggcccgg
     1321 ccttcttcac gacaggggca aaggaaaggg tgatctcctg ccgggattgc ggcgtctccc
     1381 gctggaaaag ctgggccttg gccggcgcgg cggcgagcaa aaaaacgaaa gcggcggcaa
     1441 gcggcaggcg aatggtcatg ggttgatccc gatcagcgtc aggcagggac tctgcgccta
     1501 tcacacggcg aaagtttgga gaatgcaaag ctgcgattga ggccgctgtc accgggctgt
     1561 catgttggtc acctagagca tcgtcaggat cctcgatccg gaaagttcaa cgggttccaa
     1621 acagggagtt tcccatgctg aaccgcatcc ttaccgccgg cgttgcgacc gctgcgctgc
     1681 tggccctgtc gtcagcgccc gctctcgcgc aggccaagac catcgccatc gacggctcct
     1741 ccaccgtgtt ccccgtgatg gaagcgatcg ccgaggagtt ccagaaatcc aagaagggcc
     1801 aggtgcgcgt caccgtcggc atttcgggca ccggcggcgg cttcaagaaa ttctgccgcg
     1861 gcgagaccga cgcctccaac ggctcgcgcc cgatcctcaa ggcggaaatc gaggagtgca
     1921 agaagaacgg cgtcgagttc atggaactgc cgatcgcctt cgacgcgctc accgtcgcgg
     1981 tcagcccgaa gaacaccttc cttcagtgcg tcaccgtcga gcaactcaag aagatctggg
     2041 agccttcggc ccagggcaag atgatgaagt ggaaggacgt cgatccgtcc tggccggatc
     2101 agcccatgaa gctgttcggc gcgggctcgg attcgggcac gttcgactac ttcaccgaag
     2161 cgaccgtcgg caaggcgaag gccagccgcg gcgacttcac ggcttctgaa gacgacaacg
     2221 tgcttgttca gggcatcgag accgacgcca actcgctcgg ctacatcccc ttcgcctact
     2281 acgcgccgca tgccgccaag atgaaggccc tcgccattga ctggggcaag ggccagggct
     2341 gcgtgaagcc ttcgctggaa aacgttctgg ccggcaccta caacccgctt tcgcgtccgc
     2401 tgttcatcta catcagcaag aagtccgcgg ataagccgga agtgaaggag tttgtcgagt
     2461 tcgcgatgaa gaatgccgtt ccccttatca aggaagtgaa gtatctgccg cttcctgatc
     2521 gcgcctaccc agcggcgctg cagcgcttcc agaagggtgt caccggatcg gccttcggcg
     2581 gcgtgcctga agtgggcgtc tccatcgacg agttgctgaa gcgcgaaccg aaggtctgac
     2641 cttcgctcac gcgcgagatc gaagacggcg ggcggcaaaa ccgcccgtcg tttcatccgg
     2701 atgaagtggt caggactgca ccatggcgac gagaagaacg ccccaggaac tgcggaaaat
     2761 gcgcgcccgc gagcgggcga tcgagtgggc cctgttcgca gctgcggcat catcagtcgc
     2821 cgtcacgatc gccatcgtgg ccgtcctcct ctacgaatcg ctgggtttct tcgcccatgt
     2881 cccactgggc accttcctca ccgacacgat gtggacgccg cttttcgccg atccgcgtta
     2941 tggcgtgctg ccgctccttg gaggaacgct gctctccact ttcgtcgcca tgcttgtggc
     3001 aggcccgctc ggcctgacag cggcgatcta tctttcggaa ttcgccagtt cccgcgtgcg
     3061 tgaagtcgtc aagccgacgc tggaactgct ctccggcgtt cccacagtcg tctacggctt
     3121 ctttgccctg ctgttcgtca caccggcgct gcaggcgatc ataccgggct tgccgggctt
     3181 caacgtcctg tcagccggca tcgtgatggg gatcatgatc attccctaca tcgcatccct
     3241 gtcagaggac gcgatgcgcg ccgttcctaa cggcctgcgc gacgggtcgc tggcactcgg
     3301 agcaacccgg ctggagaccg ccctcagggt ggtgctgccc tcggcattct ccggcgtcgc
     3361 ctccgccttc gtgcttggca tctcccgcgc cgtcggcgaa accatggtcg tcgccatcgc
     3421 cgccggccag cagcccaatc tcaccatcga cccgcgcgaa ccggccgcca ccatcacggc
     3481 ctacatcgtc caggtcgcgc tcggcgatct tccacacgga tcggtcggct accagacgat
     3541 cttcgccgtc ggtcttgtcc tgctcctcat gaccctcttc ttcaacgtcg ccgccttctg
     3601 gctccgcgcc aagttccgtg aggcctactg atgtcccgcg cagacaccgt aaccgtcgct
     3661 gctccggcga aagcggcact gggcaaggga attcccgcac ggcgccgaag ggagaagatt
     3721 ttcaatgcca tcggcatcgg ctcgactctt gtcggcctga ttgccctttc gctcctgctt
     3781 ttcgacctcg cctatcatgg cctgccgcgc atctcgtggg acttcttcgt caacttccca
     3841 tcgcgccggg ctacccaagc cggcattctc tcagcctggg tcggctcgct gttgatcatc
     3901 gtcgtcactg cggtcttcgg cgtgaccctc ggcgtctcgt cgggcatcta ccttgaggaa
     3961 tacgccaaac ggaactgggt gaccaacatc atcgaggtca acgtcaataa cctcgcaggc
     4021 gtgccctcca tcgtcttcgg cctgctggcg ctcggcctgt tcgtctacgg ccttgggaca
     4081 ggccgttcga tcctgaccgc tggcctcacg ctggggttgc tgatcctgcc gatcatcatt
     4141 gtcggcaccc gcgaggcgat ccgcgccgtg ccgcagagcc tgcgcgaagc ggcgtatggc
     4201 gtcggcgcca cgaaatggca ggtgacgcgc gaccacgtgt tgcccgccgc cacccccggc
     4261 atcctgaccg gagtcatcat cggcatttcg cgtgccctcg gcgagacagc gccgctgatt
     4321 accatcggcg cgctgacctt catcgccttc ctgccgacct ccccggtgtc ggacaccttc
     4381 ccgttcattt ccttccagtg gctgttcgac ggcttcaccg tactgcccat ccaggccttc
     4441 aactggattt cgcggccggg ccacgacttc cacgccaatg ccgcagcggc aggactcgtg
     4501 ctgctggcca tcaccctcag tctcaacgcc atcgccatca tcctgcgcta tcgcctgcgc
     4561 aagaaactcc agtggtgacc gggagaattc gcatgaccac gaccgacatc gccgcgacca
     4621 ccgtctcaga aggatccgtc gcggaagcac ctatcaagat cgacatcgac aaggtcagcg
     4681 tcttctacgg cgaaaagcag gccatcaaga gcgtatccac gcgcattccg gcccgcgccg
     4741 tgacctcctt catcggccct tccggctgcg gcaagaccac gctgcttcgc tccataaacc
     4801 gcatgaacga caccattccg ggctgccggg tgaccggtcg cctggcgatc gacgggcagg
     4861 acatctacgc gccctccatc gatgtggtgg agttgcgctc gcgcgtcggc atggtgttcc
     4921 agaagccgaa ccccttcgcc aaatcaatat acgagaacgt cgcgtacgga ccgcgcatcc
     4981 atggccttgc ccgctccaag acggagatgg acgagatcgt cgagtccagc ctccgtcgcg
     5041 ccagcctctg ggacgaggtc aaggaccggt tgaaccagcc cggcagcggc atttcgggtg
     5101 gccagcagca gcggctttgc attgcgcgcg ccatcgctgt cagcccggac atcattctga
     5161 tggacgagcc ctgctccgct ctcgacccca tcgccacggc gaaaattgag gaactgatcg
     5221 acgaactgcg ggtcaactat accatcgtca tcgtcaccca ctcgatgcag caggcggcgc
     5281 gcgtctccca gcggacggtg ttcttccacc tcggcgacat cgtcgaggac ggaccaacaa
     5341 ccgaaatctt cacctcgccg cgcgaaaaac gcacccagga ctacatcacc ggccgcttcg
     5401 gctgagacca caggagcgcc ccatggccga acacaccgtc aagtccttcg accaggaact
     5461 caaggacctc gccgcgcgca tttccgagat gggcggcatc gccgaatcca tgctgtcgcg
     5521 cgccgtcgag tcgctggtga agggcgatac gcacctcgcc gaggtcgtca ttcaagccga
     5581 tctgcgcctc gatgcgctgg agcgcgaggt ggccgacaag gccgtcctgg tcattgcccg
     5641 gcgccagccc atggccgacg acctgcgctt catcgtctcg tccatcaagc tggccgccga
     5701 catcgagcgg atcggcgacc tggccaagaa tatcggcaag cgcgtgaagg cgatggggca
     5761 cccgttcctt cagaagtcgc tgctgtccgg catcacccat ctcggcgatc taaccctgga
     5821 gcgcctgcac caggtgctcg acgcctatgc gaacagcgat gtcgctgcgg ccctggacgt
     5881 ctggcagcgc gacgacgata tcgacgccct gcacaattcc ctgttcaggg aactgttgac
     5941 ctacatgatg gaggacccgc gttcgatcag cgtctgcgcg catctcctgt tctgcgccaa
     6001 gaacatcgaa cgtatgggcg accactccac gaacatcgcg gagatcatcc actacatggc
     6061 gagtggcaag ccgttgttgc aggaccgccc gcgccagggc gcggtgccgg acagcgacgc
     6121 gccggccggg aaataatccg gcacgagcca gtcggagcgg cgggttctca gggaagcaat
     6181 gaaaaagggc ggctcgaagc cgccctattc aattcaaaat gttccgcgac gggagccgct
     6241 tcaggccgcg gcttccgccg gcgcttcctt ctgctgggcg gcgtggcgct ccagatcggc
     6301 cttgcccttg gcatcaacgt cgcggtccac gaactcgatc accgcgagcg gagcattatc
     6361 gccatagcgg aagcccgcct tcaggacgcg cgtgtagccg ccgttgcgct ccttgtagcg
     6421 cgggccgagc acgtcgaaga gcttcttgac gacatccgca tcgcgaatct gcgcaatcgc
     6481 ctgacggcgc gcatgcagat caccgcgctt gccgagcgtg accagctttt ccaccaccgg
     6541 gcgcagatcc ttcgccttcg gcagggtggt gacgatctgc tcgtgcgtga tcagcgcctg
     6601 agccaggttc gcgaacatcg ccttgcggtg tgacgccatc cggttgaacc ggcggccctt
     6661 gttgccgtga cgcattggct tactccatcg ttatcgcggc cgccgtgcca aggtacggcc
     6721 gtcagttatg ggcggaccgg ccgggagccg gcaaagcctg ccccaagccg gcaagagccg
     6781 cctcagtagt gttcctcgaa gcgcttggcc agttcctcga tgttctcggg gggccagccg
     6841 gtgacttcca tgccgaggtg caggcccatc tgagccagca cttccttgat ctcgttgagt
     6901 gacttgcggc cgaagttcgg cgtgcgcagc atctcggctt ctgtcttctg gatcaggtcg
     6961 ccgatgtaga cgatgttgtc gttcttcagg cagttcgccg agcggaccga cagttcgagt
     7021 tcgtccacct tcttgagcag cgcagggttg aaggcgagct ccggaatggc cgccggggcc
     7081 tcttccttgc gcggctcctc gaagttgagg aagacttcca gctggtcctg cagaatgcgc
     7141 gcggcatagg ccagcgcatc ctcgggcgtc accgagccgt cggtctcgac cgacatcgtc
     7201 agcttgtcgt aatcgaggat ctggccggac cgggtgttct cgacgcggta ggagaccttc
     7261 ttcaccggag agaacaggct gtcgaccgga atgagtccga tcggcgcgtc ctccggccgg
     7321 ttgcgatcag ccggcacata gcccttgccc gaagcgaccg tcagttccat gcggatctcg
     7381 gcgccctcgt cgagcgtgca gatcaccagc tcgggattga gcacctgggc atcgcccgtc
     7441 atctggatgt cgcccgccgt gaccgcgccg ggaccatgct tgcgaagcgt cagccgcttg
     7501 ggctggtccg actgcatctt gatcgcgatg tccttcacgt tgaggacaat gtcgatcacg
     7561 tcctcgcgca cgcccgggat cgacgaaaac tcatgcagca cgccgtcgat ctggatcgag
     7621 gtgaccgcag cgccctggag ggacgacagc agcacgcgcc gcagcgcgtt gccgagggtc
     7681 atgccgaagc cgcgctcaag cggctcggcg acgaccgtcg ccagacggcg cggatcggcg
     7741 ccgggagaaa cctccagctt gttcggcttg atcagttcct gccaattctt ctggatcacg
     7801 ggaaagtcct tccgaatctc gcgaaaaccg gcagccgtta cgcctggctg ccggaccctg
     7861 agacaacaaa caaaatcaga cgcgacgacg cttgcgcgga cggcagccgt tgtgggcgat
     7921 cggggtcacg tcacggatcg aggtaaccgt gaagcccgaa gcctgcaggg cgcgcagcgc
     7981 ggactcacgg cccgatccgg ggccgcacac ctcgacttcg atggtgcgca tgccgtgctc
     8041 gctggccttg cgggccgcat cctcggccgc cacctgcgcc gcatagggcg tcgacttctt
     8101 cgagcccttg aaaccgagtg caccggccga cgaccacgag atcgtgttgc cctgcgcatc
     8161 ggtgatcgtg atcatcgtgt tgttgaacga cgcgttcaca tgggccacgc ccgacgcgat
     8221 gttcttgcgt tcacgacgcc gaacgcgggt tgcttccttc gccatacccg aaaccttctc
     8281 ttgcctgtgc cgccccgaaa gaggcgcgcc gggtcagtga cccaatggat tacttcttct
     8341 tgccggcgat cgccttcggc ttgcccttgc gggtgcgggc gttcgtatgc gtgcgctggc
     8401 cgcgaaccgg caggccacgg cgatgacgca ggccgcgata gcagcccagg tccatcagcc
     8461 gcttgatgtt catggcggtg tcgcgccgaa gatcgccctc gaccgtgtag tcgcggtcga
     8521 tggtctcgcg aatctgcagg acttccgcgt cggtcaactg gttgacccgc ttgtcatgcg
     8581 gaatgccgac cttgtcgcaa atctccaccg cattcacggg gccgatgcca tggatatagc
     8641 gcagcgcgat caccacgcgc ttctgggtcg gaatgttgac gccggcaata cgagccacga
     8701 cgttatctcc tcaaggcctg gtctgcggcg acgaccgccg cgtcaaaggc tgcgaccgat
     8761 cctcgcggaa agccgagccc ttcatgaaat ccgtccggcg cctgccgggg tcgcgggaga
     8821 gaatcccccg ccgctccggc aaaaacagaa cgcgcggaaa accgcgcgtc tgctcacctt
     8881 gttcggtagt tgggagcgga tagccgcgca cgcgcgactc gtcaaccccc aacaaccgaa
     8941 ttctccggcc gccgcgccag gaaagtgcgc gcgcggcccc attcctcaca gcgccgccag
     9001 cacctgcatg agcgcggcgg tcacgccatc gatcggctgc atgccgtcga ctgtcttcag
     9061 catgcccttc gcccggtagt agggcgtcac cgctgccgta tcgcggtgat aggccgcgag
     9121 gcgtgtcttg aacacttcgg gatcatcgtc cttgcgcacc ggctggcctg ccttgcgggc
     9181 atcctccgcg cgcttgacga tacgatcgac cagcttggtc tcgtcgacga ccagttccac
     9241 acagcccgac agcttcacgc ccttgcgcga cagcatggcg tccagcgctt ccgcctgggc
     9301 gacggtgcgc ggaaaaccat ccaggatgaa gcccttctgg gcgtcactct ggccgatccg
     9361 ctcctcgacg atgccaacaa cgatctcgtc ggagacaaga ccgccggatt ccattacagc
     9421 cttggccttc agcccgacgg gcgagccagc cgcaacggcc gcgcgcagca tgtcgccggt
     9481 ggacaactgc ggaatgccgt acttctcgac aatccgttgg gcctgcgtgc ccttccccgc
     9541 ccccgggggt cccagcagaa tcagcctcat ttacgctttc ccttgagctt gttcctcttg
     9601 atcaggccct cgtactggtg cgcgagcagg tgaccatgca cctgcgcgac cgtatccatg
     9661 gtcaccgaaa cgacgatcag gatcgaggtg ccgccaaaat agaacggcag gccgctctgg
     9721 gaaacgagca attcgggtaa gagacagatc agcgtcaggt acagcgcgcc gaccacggtg
     9781 atgcgcgaca gcacgtgatc gatgtactgc gccgtgcgtt caccgggccg aatgcctggg
     9841 atgaagccgc cctgcttctt caggttgtcc gccgtctcga caggattgaa cacgatggcg
     9901 gtatagaaga agcagaaaaa gatgatcagc gccgcataga gaatcatgaa cagcggccgg
     9961 ccgtggccaa ggtaggcgtt catggtttgc agccactccg ggccgccaga ctgggcaaag
    10021 cccgtgaccg tggtcggcag cagcagcagc gacgaggcga agatcggcgg aatcacgcca
    10081 gaagtgttga gcttgagcgg caggaacgag gtctgcccct cgtagacccg gttgccgacc
    10141 tgccgcttcg gatagttcag cagcaggcgg cgctgcgcgc gctcgacgaa cacgatcagc
    10201 gcgatcacgg cgatgcccat gaccatcgtc agcaggatca ggcccgtgga cagcgcaccc
    10261 tggcgcgaca gttccagcgt gccagcaatg gccgacggca gttccgcgac gatgccggcg
    10321 aagatgatca gcgacgtgcc gttgccgatg ccacggctgg tgatctgctc gccgagccac
    10381 atcatgaaca gcgtgccgcc ggtcagtgtg atcaccgtgg acaggcggaa gaaaaggccg
    10441 gggtcgcgta ccacattgcc cgaggcttcc agcccgaccg agattccata ggcctggaac
    10501 agcgccagca ccacggtgag atagcgcgtg tactggttga gctgccggcg ccccgattcg
    10561 ccctctttct tgagcgcttc gagctgcggg ctgaccgtcg tcagcagctg gatgatgatc
    10621 gatgccgaga tgtacggcat gatgttgagc gcgaagatcg ccagtcgccc gacggcgccg
    10681 cccgaaaaca tgttgaacag gccgagcacg cccgacatct gcgtcttaaa aatctcggcc
    10741 accgcctccg gattgatgcc gggcagcggg atgtaggtgc cgagacgata gaccagcagc
    10801 gccccgagcg tgaaccagat gcgcttcttc agctcctccg ctttcgcgaa agcgccgaag
    10861 ttgaggttgg ctgcgaattg ttctgctgcc gatgccatgc gtccgttccg ggggaagggt
    10921 cggccggtct gccggacgcc cgatatcgct gagggtacgc cctagatagg ttcgccgcgg
    10981 ccggttatcc agccgcggcg ttccatttgt gatcaggcct gcgccggagc ttccttggcc
    11041 ggagccagca gcttgacgct gccgccggcc ttttcgatcg ccgccagggc gctcttggaa
    11101 gcgccataga cttcgaacgc cagcttggcc ttgagttcgc cctgcccgag gatcttgacg
    11161 ccgtcgcgcg cccgggcgca aacgcccgcc gcgacgagag ccgcctcggt gaccggggcc
    11221 ttggcgtcga gcttgccagc atccaccgcc tgctggacgc gcccgaggtt cacctcgttc
    11281 aggtccttgg cgaagatgtt ggtgaagccg cgcttcggca ggcgccgatg cag
//
LOCUS       DAOMXN010000057        11269 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_3100_length_11269_cov_11.096219, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000057 DAOMXN010000000
VERSION     DAOMXN010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 11269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11269
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3100_length_11269_cov_11.096219"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="PK812_06140"
     CDS             complement(<1..205)
                     /locus_tag="PK812_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012253182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69171.1"
                     /translation="MSEDTIFALSTGVGRAAIAVVRASGPAVRLALDEIDRSDELIKA
                     FVSVDRSARAQASGPLRGVTFGVK"
     gene            299..2311
                     /locus_tag="PK812_06145"
     CDS             299..2311
                     /locus_tag="PK812_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRJ69172.1"
                     /translation="MTNRLSQEVSPYLLAHADNPVDWYPWGEEAFAEARRTGRPVLLS
                     IGYSACHWCHVMAHESFEHAETAVLMNDLYVNIKVDREERPDVDHVYMAALQSLGQHG
                     GWPLTMFLDATGKPFWGGTYFPREARYGQPAFRQVLTSVADAYRNRRNDIESNGRAIL
                     AALSDRTPADDPAALSLTLLDRLAARIRDLHDPVNGGLKGAPKFPNPPVLEFLWRAAD
                     RTGDESVRSAMTLTLERMVRGGIHDHVGGGFARYSVDERWLVPHFEKMLYDNAQLLPL
                     LALAAQRSGSEQLSHAATRLVEWLLREMRTAGDAFASAQDADSLDASGHSEEGAFYTW
                     TPAEIRTILGAEADLWNAAYDITRDGNFEGRSIPNLLARHGGERPEAAIDPVTDRLRS
                     ARALRPPPARDDKVLADWNGLMIAGLTRAAALLGRPDWIAPAQAAFAAVVDHLGGESV
                     LAHAWRNGRRVHPGFASDHAAMGLAALALMEATGETRYLAFAEAWAATLLSDYLMPDT
                     GLIAMSIATANDLPIRPAPTHDDAMPNANAMTADLLIRLYGLTGEDRWLAAADDLLAA
                     ATPAALANPFGHAALLNALDLRLRRADIVAVEPGASHLTDAALNVPFTNRTLARVAPG
                     SPLPRTAAAPDGFQAPYALVCAQGRCSLPVSDAASLRLRIEEALRA"
     gene            complement(2395..3666)
                     /gene="rho"
                     /locus_tag="PK812_06150"
     CDS             complement(2395..3666)
                     /gene="rho"
                     /locus_tag="PK812_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008191409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HRJ69173.1"
                     /translation="MREIKLHDLKAKSPTELLSFAEEVEVEGASTMRKQELMFAILKQ
                     LASREVEIIGEGVVEVLQDGFGYLRAPDANYLAGPDDIYISPSQIRRFALRTGDTVEG
                     QIRSPREGERYFALLKVNRINFEDPEKARHKVHFDNLTPLYPNERIKLEVEDPTRKDM
                     SSRVIDIVSPIGKGQRALIVAPPRTGKTVLLQNIAKSITANHPECYLIVLLIDERPEE
                     VTDMQRSVKGEVISSTFDEPATRHVAVAEMVIEKAKRLVEHGRDVVILLDSITRLGRA
                     YNTVVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTIISTALIDTGSRMDEVI
                     FEEFKGTGNSEIILDRKVSDKRVFPAIDITRSGTRKEELLVPHDVLKRMYVLRRILNP
                     MGTVDAIEFLLDKLSKTKQNSEFFDSMNTRD"
     gene            complement(3864..4289)
                     /gene="hemJ"
                     /locus_tag="PK812_06155"
     CDS             complement(3864..4289)
                     /gene="hemJ"
                     /locus_tag="PK812_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase HemJ"
                     /protein_id="HRJ69174.1"
                     /translation="MYLWIKALHVVAVISWMAGMLYLPRLFVYHCDAPIGSQQSETFK
                     VMERRLLRAIINPAMIVAWLTGLWMAYEAFSFRGGWLHAKLFLVFFLMSGFHGYLARW
                     RKDFEADRNLRSATFYRIMNEVPTLGMIAVVILVIVKPF"
     gene            complement(4292..5305)
                     /gene="hemE"
                     /locus_tag="PK812_06160"
     CDS             complement(4292..5305)
                     /gene="hemE"
                     /locus_tag="PK812_06160"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HRJ69175.1"
                     /translation="MFLETLHGASGSPPPVWLMRQAGRYLPEYREIRTRAKSFLEFCY
                     TPDLAIEATLQPIRRFGFDAAILFADILVVPDALGQTVRFMEGEGPRLEPLVDDKGFE
                     FLHRKIDLARLAPVMSTIRGVRAALAPDKALIGFCGAPWTVATYMVAGRGTPDQKPAR
                     MLAYRKPQLFARLIDRLVDASVDYLAEQFSAGVDCVQIFDSWAGVLPELEFERWSLEP
                     TRRIVEKLRQRVPGAAVIGFPRGASSQLVQYAAETGVNAVGVDTAVNMGFVARDVPKN
                     VAVQGNLDPLVLIAGGEALDRAVDVILDSTRGRPHIFNLGHGILPETPIAHVERLLKR
                     IRG"
     gene            5696..6526
                     /locus_tag="PK812_06165"
     CDS             5696..6526
                     /locus_tag="PK812_06165"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HRJ69176.1"
                     /translation="MGRSFFHLHLVSDSTGETLLAVSRAASAQYQGISAIEHIYPLVR
                     NAAQLDRVLEEIELAPGIVLFTLVDKTIADRLQAACREAGCPCLSVLEPVHSLFQSYL
                     GIATTPKAGAQHTLNADYFRRIDALNFTMMHDDGQLTDDIDQADVILVGVSRTSKTPT
                     SIYLANRGIKTANVPLVPGMPIPDRLLTLVRPLIVGLVASPERIVQIRQNRLLSLNAG
                     DETSYVDKSSVAEEVAQSRRLFEKHDWPIIDVSRRSIEETAAAILDLYRNHRLTLMAQ
                     "
     gene            6523..7143
                     /locus_tag="PK812_06170"
     CDS             6523..7143
                     /locus_tag="PK812_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HRJ69177.1"
                     /translation="MSRPYALWRAGPPLLLASASTVRRRLLEECGIPVEATPAGLDER
                     AAEACLADRSPSMLARGLAEAKARAVSARSAGRLVLGADQTLALADTIYHKPRDIAEA
                     RRHLEAFSGRTHHLHSGLALVRDATVLWAHVESATLHVRTLSRAFIDGYLEAAGADVL
                     ASVGAYRLEAHGAHLFERIEGEHSTILGLPLRPLLAELRRQGCLLA"
     gene            7140..8012
                     /locus_tag="PK812_06175"
     CDS             7140..8012
                     /locus_tag="PK812_06175"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HRJ69178.1"
                     /translation="MMTQATPGPKAAVIGKPIAHSRSPLIHGYWLRTYGLPGSYGRVL
                     VEPEDLPVFLRDLAANGYAGCNVTIPHKEGAFAACASHTPTAAALGAVNTLWFEDGML
                     WGDNTDVLGYQASLDAAAPGWSDNVRCARLLGAGGAARAIVFGLAERGVRRIEIANRS
                     EDRAAALSQDLAPLAARFGASLEVIGWDRRDAGLAGVQHLVNTTSLGMVGQPPLEIDL
                     AGLPEGAIVFDAVYVPLVTPLLARAKARGLVVADGLDMLLHQARPGFERWFGRAPQVT
                     EELRRLAEADLAGA"
     gene            8016..8615
                     /gene="coaE"
                     /locus_tag="PK812_06180"
     CDS             8016..8615
                     /gene="coaE"
                     /locus_tag="PK812_06180"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386105.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HRJ69179.1"
                     /translation="MLVVGLTGSIGMGKSATAALFREAGVPVDDADAAVHRLYAGRAA
                     PLIEARFPGVTEAGVVDRARLSAAVLGNPEALTALEAIVHPLVKQEREAFLDAARKSG
                     ARLVVLDIPLLLEGRSEGLVDLVLVVSAPEPVQKARVLARPGMTEAKLAAILARQMPD
                     ADKRRRAHWVIGTATGIKAARRDVQTFLRAAAAMTGHPR"
     gene            8612..9316
                     /gene="dnaQ"
                     /locus_tag="PK812_06185"
     CDS             8612..9316
                     /gene="dnaQ"
                     /locus_tag="PK812_06185"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HRJ69180.1"
                     /translation="MTREIVLDTETTGLDPKKGDRIVEIGCVEIVNRFLTGRTFHRYL
                     NPEREMPPDAFAVHGLSDGFLRDKPKFIEVADEFLEFLGDAQLVIHNAAFDIGFLNFE
                     LGRVSRPTIPVERVVDTLMLARRKHPGQPNSLDALCQRYGVDSSKRTRHGALLDSELL
                     AEVYAELTGGRQQTFALAQLAVRDVNLSAGARVRRQPIQPELSEAERTAHRAFVEGLG
                     PKASWRTYLGLDEGKT"
     gene            complement(9324..9815)
                     /gene="secB"
                     /locus_tag="PK812_06190"
     CDS             complement(9324..9815)
                     /gene="secB"
                     /locus_tag="PK812_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HRJ69181.1"
                     /translation="MNTTPNNGAGQDTSPHLGALAQYIKDLSFENPHAPKSLQSPGGQ
                     NPNIQVQVNVNARQIMGEDYEVELKLEGTAGQQPELMFQFELNYAGVFRLRNIPQEHI
                     GGVLMVECPRILFPFARQIVANAVRDGGFPPLYLDPIDFLALYQQRVAELNQQQPAGV
                     RPS"
     gene            complement(9873..10337)
                     /locus_tag="PK812_06195"
     CDS             complement(9873..10337)
                     /locus_tag="PK812_06195"
                     /inference="COORDINATES: protein motif:HMM:NF016102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HRJ69182.1"
                     /translation="MIVRRWHLRMAALGVLGWIIAEALLFYAATRSIGVMATILFMAL
                     KGLGGLALFAIAVRTVLRNLARTGRRRGLPGAAHAGFAALGALLILVPGFLAPLLGVA
                     LFSPSARLGLLRFLKRRRERDRSVLTLDRSEWREVSARRPRRTRRGNRSLAP"
     gene            10488..11195
                     /locus_tag="PK812_06200"
     CDS             10488..11195
                     /locus_tag="PK812_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44/TimA family putative adaptor protein"
                     /protein_id="HRJ69183.1"
                     /translation="MHEFLDVYTLVFLALAIFVIFRLRAVLGQRTGHEKPPFDPFQRP
                     EPPKPEQPSNVIPLPQAAQRTAPEVAADPAAILKGIAQPGSPVAQGLTQLMATDSSFD
                     PRQFVSGARAAYEMIVLAFARGDRKALKDLLSKEVFDNFVAAIAGREQRGETVETTFV
                     SIDRSEVVEAELKGNSMQVTVKFASKLITATRNASGALIEGSAERIADLVDVWTFARD
                     ANSRDPNWRLISTEADE"
BASE COUNT         1910 a         3822 c         3573 g         1964 t
ORIGIN      
        1 ccttcactcc gaaagtgaca cctcgcagag ggcccgacgc ttgtgcgcgc gcactgcgat
       61 cgacactgac gaaggccttg atgagttcgt cgctccgatc gatttcgtcc agagcgagcc
      121 gaaccgcagg cccggaggcg cgcaccaccg cgattgcggc ccggccgacg cctgtgctca
      181 gggcaaaaat cgtatcctcg ctcacgcagc gcttcctctg ccccggatca tgccccaaga
      241 ccatcgccag ccgggcgcat caaccctata tcccgacatc accgagcgga tcagtcccat
      301 gaccaatcgc ctttcccagg aagtcagccc ctatctcctg gctcacgccg acaaccccgt
      361 cgactggtac ccctggggcg aagaggcctt cgccgaggcg cgccgcaccg gccggccggt
      421 gttgctctcg atcgggtatt cagcctgcca ttggtgccat gtcatggctc atgaaagctt
      481 cgaacatgcc gagactgcgg tgctgatgaa cgatctttat gtgaacatca aggtcgatcg
      541 cgaggagcgg ccggatgtcg accatgtcta catggccgcg ctgcagagcc tcggccagca
      601 cggcggctgg ccgctgacca tgtttctcga tgcgacaggc aagccgttct ggggcggcac
      661 ctactttccg cgcgaagcgc gctatggcca gcctgcattc cggcaggttc tgacctccgt
      721 cgccgacgcc tacaggaacc gccgcaatga catcgaatcg aacgggcgcg ccatcctcgc
      781 cgccctgtcc gatcgcacgc ccgctgacga ccctgccgcc ctgtccctga cgctgctgga
      841 ccgtcttgcg gcccgcatcc gcgatctcca tgaccccgtc aacggtggcc tgaagggcgc
      901 accgaagttc cccaatcctc cggttctgga attcctctgg cgcgcggcgg accggaccgg
      961 cgacgaatct gttcgctccg ccatgaccct gaccctcgaa cggatggtcc gggggggcat
     1021 ccatgatcac gtcggcggcg ggttcgcgcg ctattcggtc gacgagcgct ggctggtgcc
     1081 gcatttcgag aagatgctct acgacaatgc ccagctgctc ccgctcctcg ccctcgcggc
     1141 gcagcgctcc gggtccgagc agctctccca tgccgccacg cgcctcgtcg aatggctgct
     1201 gcgcgagatg aggacggccg gcgacgcctt tgcctcggcc caggacgccg acagcctcga
     1261 tgcttccgga cactccgagg aaggggcgtt ctacacctgg acgcccgcgg agatccgcac
     1321 cattctcggc gccgaggccg atctctggaa cgccgcatac gacatcacgc gcgacggaaa
     1381 ttttgaaggt cggtccatcc ccaatcttct cgcccggcac ggcggcgaac gaccggaagc
     1441 cgcgatcgac cctgtcaccg accgcctgcg ctccgcccgc gccctgcgtc ctccaccggc
     1501 gcgcgacgac aaggttctag ccgactggaa cgggctgatg atcgccggcc tgacccgggc
     1561 ggcggccctg cttggacgcc ccgactggat cgcgccggcg caagccgcct tcgcggccgt
     1621 cgtggatcat ctcgggggcg aatcggtcct ggcgcatgcc tggcggaatg gcaggcgggt
     1681 gcatcccggc ttcgcgtcgg atcatgccgc catgggcctc gcggcgctgg cgctcatgga
     1741 agccaccggc gagacccggt atcttgcctt tgccgaagcc tgggccgcca ccctgctgtc
     1801 ggactacctc atgccggaca ccggcctcat cgccatgtcg atcgccacgg caaacgacct
     1861 gccgatccgg ccggcgccca cccatgacga tgcgatgccc aacgccaatg ccatgacggc
     1921 ggacctgctg atcaggctgt atggactgac cggtgaggac cggtggcttg ctgcggccga
     1981 cgatcttctc gccgccgcga cgccagccgc gctcgccaat ccgtttggtc acgccgctct
     2041 gttgaacgcg ctcgatctgc gcctgcgccg ggcggatatt gtcgctgtcg aacccggcgc
     2101 gtcgcacctc accgatgccg cgcttaatgt tccgtttacg aatcggaccc tggccagggt
     2161 cgcgcccggc agtccgctac cgaggaccgc tgccgctccg gacgggttcc aggcgcccta
     2221 cgcgctcgtc tgcgcccagg gccgctgctc cttgccggtg agcgatgccg catcgttgcg
     2281 tctgcgaatc gaggaggcgc tgcgcgccta gccgcgcaga cacccaacgc ctgcgcagct
     2341 tgcaccaagg gagacgaccg gcggggttcc tggccccgtc acaacggtcg ttcctcagtc
     2401 ccgtgtgttc atcgagtcaa agaactcgct gttctgcttg gtcttggaca gcttgtccaa
     2461 gaggaactcg atggcatcga cggtgcccat cggattgagg atacgccgca gcacgtacat
     2521 ccgcttgagc acgtcgtgcg ggaccagcag ctcctccttg cgggtgccgg atcgggtgat
     2581 gtcgatggcc gggaacacgc gcttgtccga caccttccgg tccaggatga tttccgagtt
     2641 gccggtgccc ttgaactctt cgaaaatcac ttcgtccatg cggctgcccg tatcgatcag
     2701 cgccgtcgag ataatggtca gcgagccgcc ttcctcgatg ttgcgcgccg cgccgaagaa
     2761 cctcttgggc cgctgcaggg cgttcgcatc gacgccgccg gtcagcacct tgccggacga
     2821 cggcaccacg gtgttgtagg cccggccgag acgtgtgatg gaatccagga ggatcaccac
     2881 gtcgcggcca tgttcgacca ggcgcttggc cttctcgatc accatctccg ccacggccac
     2941 gtgccgggtc gccggctcgt cgaaggtcga ggaaatcacc tcgcccttca ccgagcgctg
     3001 catgtcggtg acttcctccg gccgctcgtc gatgagcagc acgataaggt agcactccgg
     3061 gtggttggcg gtgatggact tggcgatgtt ttgcaaaagc acggtcttgc cggtgcgcgg
     3121 cggtgccacg atcagggcac gctgcccctt gccgatcggt gacacgatgt cgatcacgcg
     3181 cgacgacatg tccttgcgcg tcggatcctc gacttcgagc ttgatccgct cattgggata
     3241 gagcggcgtc aggttgtcga aatgaacctt gtgacgtgcc ttctccggat cctcgaaatt
     3301 gatcctgttg accttcagca gcgcgaagta acgctcgcct tcgcgtgggc ttcggatctg
     3361 gccttctacc gtgtcgcccg tgcgcaacgc gaagcggcga atctgcgagg gcgaaatata
     3421 gatgtcatcc gggccagcca ggtagttggc gtccggtgcc cgcaagtatc caaatccgtc
     3481 ctgaaggact tcgacgacgc cctcgccgat aatctcgacc tctcgggagg caagttgctt
     3541 caggatggca aacatcagct cctgctttcg catggtgctg gcaccttcca cttcgacctc
     3601 ttcggcaaag ctgagcagtt ccgtgggtga ttttgccttg aggtcgtgga gcttgatttc
     3661 ccgcatggga ataccttttg atcatcggcg ctgggccggc agggtggaaa agcgcaagcg
     3721 gggctttatg gaaggaaagc cgctctggcg ggcaacccgc tcgcgggaag gcgcgccgcg
     3781 gaaatgttgg ggagcgcctt catcgaaaca gcgctgaaat agtcggtttc gctggcggac
     3841 acaagggccg gcgcgccggt cgctcagaac ggcttgacga tgaccaggat caccaccgcg
     3901 atcatgccga gtgtcggcac ctcgttcatg atccggtaga acgtcgcgct gcgcaggttg
     3961 cggtccgcct cgaaatcctt gcgccagcgc gcaagatagc cgtggaagcc cgacatcagg
     4021 aagaagacca ggaacagctt ggcgtgcagc cagccgcccc ggaaggaaaa cgcctcgtag
     4081 gccatccaca gtccggtgag ccaggccacg atcatcgccg gattgatgat ggccctgagc
     4141 agccggcgct ccatcacctt gaaggtctcg gactgctgcg atccgatcgg cgcgtcgcaa
     4201 tgatagacga acaggcgcgg cagatagagc atcccggcca tccacgagat gacggcgacc
     4261 acgtgcagcg ccttgatcca cagatacatc gtcagccccg gatccgcttc agcagccgct
     4321 cgacatgggc gatcggcgtt tccggcaaga tgccgtggcc gaggttgaag atatggggac
     4381 gcccgcgcgt ggaatcgagg atgacatcca cagcccggtc gagcgcctcg ccgccggcga
     4441 tcagcaccag cggatcgaga ttgccctgta ccgcgacatt cttcggcacg tcgcgtgcga
     4501 caaagcccat gttgaccgcc gtatcgacgc cgaccgcatt caccccggtc tccgccgcat
     4561 actgcacaag ctggctgctg gcaccacgcg gaaatccgat caccgccgcg ccgggaacac
     4621 gctggcgcag cttttcgacg atccggcgtg tcggctcgag gctccagcgt tcgaactcca
     4681 gttccggcag cactccggcc caggaatcga agatctgcac gcaatcgacc ccggcggaga
     4741 actgctcggc aagataatcc acggacgcat cgaccagcct gtcgatcagc ctggcgaaca
     4801 gctgcggctt gcgataggcc agcatgcgcg ccggcttctg gtccggcgtg ccgcgaccgg
     4861 ccaccatgta agtcgccact gtccacggcg caccgcaaaa tccgatcagt gccttgtccg
     4921 gagccagcgc cgcacgcacg ccacggatcg tcgacatcac cggcgccaac cgggccagat
     4981 caatcttccg gtgcaggaat tcgaacccct tgtcatcgac cagaggctca agccgagggc
     5041 cttccccctc catgaaccgc acggtctgtc ccagcgcatc cggtacgacc aggatgtcgg
     5101 caaacaggat cgccgcatcg aacccgaagc gccggatcgg ctgcaacgtc gcttcgattg
     5161 ccagatcggg cgtgtagcaa aactcgagga acgatttcgc tctcgtgcgg atctcccggt
     5221 actccggcag gtagcggccg gcctggcgca tcagccagac cggtggaggc gatccgctcg
     5281 ctccgtgcag ggtttcgagg aacaatcccc ggcgtttcga tccattcaat aaaagagaag
     5341 acaagaaaga ctcttttctg attcgattct taggaccatc gaatccgatg gcgctgtgtt
     5401 ttccccaacc aatccccagc cggcgcggga tggcaccgga gtgggcaggc agcgccgttg
     5461 ccacggttca ccgcgcatct tctggcactt ttgccgctcc agccgcaaga ggccaggcga
     5521 agcaccgact atccccgctt cacccatttg atcgcgtccc gcatatgttc tttgtggacg
     5581 ggtctgtgta tgacaaccgc gttttcccct gccggtctgc cgccggcgga ccagtggccg
     5641 ggatatcccg gcttgtccac aggacccggg catgcccggc agcgacggag tcatgatggg
     5701 ccgcagcttc ttccatctgc atctggtgtc cgattccacg ggcgaaaccc tgctggccgt
     5761 aagtcgcgcc gcctccgccc agtaccaggg catctccgcc atcgagcata tctatcctct
     5821 cgtgcgcaac gcggcgcagc tcgaccgcgt gctggaggaa atcgaactgg cgccgggaat
     5881 cgtgctgttc acgctggtcg acaagaccat cgccgatcgc ctgcaggcag cctgccgcga
     5941 ggccggctgt ccctgcctgt ccgtgctgga acccgtgcac tcgctgttcc agtcctatct
     6001 tggtatcgca accacgccca aggccggtgc acagcatacg ttgaacgcgg actacttccg
     6061 ccgcatcgat gcgctcaact tcaccatgat gcacgacgac ggccagctca ccgacgacat
     6121 cgaccaggcc gatgtcattc tcgtcggcgt cagccgcacc tcgaagacac cgacgtcgat
     6181 ctatctggcg aaccgcggca tcaagacggc caatgtgccg ctggtgccgg gcatgccgat
     6241 tcccgaccgt ctgctgaccc tcgtgcgtcc gctgatcgtc gggctcgtcg ccagtccaga
     6301 gcgcatcgtg cagatccggc agaaccgcct gctgtcgctc aatgccggcg atgaaacctc
     6361 ttatgtcgac aagtcgtccg tcgcggaaga agtggcccag tcccgccgcc tgttcgaaaa
     6421 gcatgactgg cccatcatcg atgtgtcgcg ccgctcgatc gaggaaaccg ctgccgccat
     6481 cctcgacctt tatcgcaatc accgcctgac gctgatggcg caatgagccg tccgtacgct
     6541 ctctggcgcg ctgggccgcc gttgctcttg gcttccgcca gcacggtgcg tcgccgcctg
     6601 ctggaagagt gcggtatccc ggtggaagcc actcccgccg gtctggacga acgcgcggca
     6661 gaagcctgcc tcgccgatcg atcgccgtcg atgctggcgc gcgggctcgc ggaggccaag
     6721 gcccgcgccg tttccgcaag gtcagccggc cgccttgtgc tcggcgccga ccagacgctg
     6781 gcgctcgccg atactatcta tcacaagcca cgcgacatcg ctgaggcacg ccggcatctg
     6841 gaagccttct ccgggcgcac gcatcatctg cactccggcc tcgcccttgt gcgtgacgca
     6901 actgtgctgt gggcgcatgt cgaaagcgcg accctgcatg tgcggacctt gagccgggcc
     6961 ttcatcgacg gctacctcga agccgccggc gccgatgtac tggccagcgt tggcgcctat
     7021 cggctcgaag cgcatggcgc gcacctgttt gaacggattg aaggcgagca ttcgaccatt
     7081 ctcggtctgc cgctgcgacc gctgctcgcg gaactgagac gccaggggtg ccttctcgca
     7141 tgatgaccca ggccacgccc ggaccgaagg ccgcggtgat cggcaagccg atcgcccatt
     7201 cgcgctcgcc cttgatccac ggctactggc tcaggaccta cggcctgccc ggcagctacg
     7261 ggcgggtgct ggtggaaccg gaggacctgc cggtcttcct gcgcgatctt gcggccaatg
     7321 gctatgcagg ctgcaacgtc acaatcccgc acaaggaagg cgccttcgcc gcctgcgcga
     7381 gccacacccc gacggccgcg gcgcttggcg ccgtcaacac gctgtggttc gaggacggca
     7441 tgctgtgggg cgacaacacg gatgtgctgg gttatcaggc cagcctcgat gccgcggcac
     7501 cgggctggtc ggacaacgtg cggtgcgcaa ggctgctggg cgcgggcggc gctgcccgcg
     7561 ccatcgtctt cggcctcgcc gaacgcgggg tacggcgcat tgagatcgcc aaccgcagcg
     7621 aggatcgcgc cgccgcgctg tcccaggacc tggcgccgct ggccgcgcga tttggcgcga
     7681 gcctggaggt gatcggctgg gaccggcgcg atgccgggct cgccggtgtg cagcatcttg
     7741 tcaacaccac ctcgctcggc atggtcggtc agccgccgct ggagatcgat ctggccggtc
     7801 tgccggaagg ggccatcgtg ttcgacgccg tctatgtgcc gctggtcacg ccgctgctcg
     7861 cccgcgcgaa ggcgcgcggc cttgtcgtcg ctgacggtct ggacatgctg ctgcaccagg
     7921 cgcgcccggg ctttgagcgc tggttcgggc gcgcgccgca ggtgaccgaa gagctgcggc
     7981 gccttgccga agccgacctg gccggagcct aggcgatgct ggtcgtgggt ctgaccggat
     8041 cgatcggcat gggcaagtcg gcgactgcgg cgctgtttcg ggaagccggc gtgccggtgg
     8101 acgatgccga tgccgcggtg caccggctct atgccgggcg ggccgcgccg ctgatcgagg
     8161 cgcgctttcc cggcgtgacc gaagccggag tggtggaccg agccaggctg tcggcggcgg
     8221 tgctgggcaa tcccgaggcc ctgacggcgc tggaggcgat cgtgcatccg ctggtgaagc
     8281 aggaacgcga ggcctttctc gacgccgcgc gcaaatccgg cgcgcgtctc gttgtgctcg
     8341 acatcccgct gttgctggaa gggcgctccg aagggctggt cgacctcgtc ctggtggttt
     8401 ccgcgccgga gcctgtgcaa aaggcgcggg ttctggcccg accgggcatg acggaagcca
     8461 agctggccgc catactcgcc cgccagatgc cggacgcgga caagcgccgc cgtgcccatt
     8521 gggtgatcgg caccgccacc ggcatcaagg cggcgcgccg ggacgtgcag accttcctgc
     8581 gcgccgcagc agccatgacg ggacatccgc gatgacgcgc gaaatcgttc tggataccga
     8641 gacgaccggt ctcgacccga agaagggcga tcgcatcgtc gagatcggct gcgtggagat
     8701 cgtcaaccgc ttcctgaccg gacgcacgtt ccaccgctac ctgaatcccg agcgggagat
     8761 gccaccggac gcattcgccg tgcacgggct gtcggacggg ttcttgcggg acaagcccaa
     8821 gttcatcgag gtcgccgacg aatttcttga attcctgggg gacgcgcagc tggtcatcca
     8881 caacgcggca ttcgatattg gctttctgaa cttcgaactg ggcagggtgt cacgcccgac
     8941 catccccgtc gaacgcgtgg tggataccct gatgctggcg cgccgcaagc atccggggca
     9001 gccaaattcg ctggatgcgc tctgccagcg ctatggcgtc gattcgagca agcgtacccg
     9061 ccacggggcg ctgctcgatt ccgagcttct tgcggaggtc tatgccgaac tgaccggcgg
     9121 tcgccagcag acgttcgccc tggcgcagct cgcggtgcgg gacgtgaacc tgtccgctgg
     9181 cgcgcgggtc cgccgccagc cgatccagcc cgaactgagc gaggcggaac gcaccgccca
     9241 tcgcgctttc gtggaaggcc tgggcccgaa ggcgtcctgg cggacctacc tcgggctcga
     9301 cgaaggaaag acctgaccgt cgctcaggac ggacggacgc ccgccggctg ctgctggttg
     9361 agctcggcga cacgctgctg gtacagcgcg aggaagtcga tcggatcgag atagagcggg
     9421 gggaaaccgc cgtcacgcac cgcattcgcc acgatctgac gggcgaaggg gaacaggatg
     9481 cgtgggcatt ccaccatcag cacaccaccg atatgctcct gcgggatgtt gcgcaggcgg
     9541 aacacgccgg catagttcag ttcgaactgg aacatcagtt ccggctgctg gccggctgtt
     9601 ccttccagct tcagctcgac ctcgtagtcc tcgcccatga tctgtctggc gttgacattg
     9661 acctggacct ggatgttggg gttctgcccg cccgggctct gcagggactt gggcgcatgc
     9721 gggttctcga aggacagatc cttgatgtac tgggccagcg cgccgaggtg cggggaggtg
     9781 tcctggccgg cgccgttatt cggggtcgtg ttcatcgaat gtgtccctga aatgaaatcc
     9841 gcgcgaggcc ttacacggaa ccggcgcgcg cctcaaggcg caagactgcg gttgccgcgc
     9901 cgcgtccggc gcggtcggcg agcggaaacc tcgcgccatt cgctccggtc gagcgtcagg
     9961 acgcttcggt cgcgttcgcg gcggcgcttg aggaaccgca gcaggccaag gcgcgcggag
    10021 ggggaaaaca acgcgacgcc gaggagcggc gcgaggaatc cgggcacgag aatcagcagc
    10081 gcccctagcg cggcgaagcc cgcatgggcg gctcccggaa ggccccgccg ccgtccagtc
    10141 cgcgccagat tgcgcagcac ggtgcgcacc gcgatggcga acagagcgag accaccaagg
    10201 cccttcagcg ccatgaacag gatggttgcc atcacgccga tggaacgcgt ggcggcgtaa
    10261 aacaacagcg cttccgcgat gatccagcct aaaacgccga gcgccgccat gcgcagatgc
    10321 cagcgtctga cgatcattgc ccctcctgtt cgtccgggcc tgcgaaaaaa cttgccgcgc
    10381 tctcgccacg gctccccgat cctcgctata taaggccgta ttcggatggc ggccaggcag
    10441 cgcggatgct gccgcgtcgc cgtccagcac cgtttcggaa cagcgcgatg cacgaatttc
    10501 tcgatgtcta caccctggtc tttcttgcgc tggcgatctt cgtgatcttt cggttgcgcg
    10561 ccgtgctcgg ccagcgcacc ggccacgaaa agcctccgtt cgatccgttc cagcgtcccg
    10621 aaccaccgaa accggaacag ccgtccaacg tcatcccctt gccacaggct gcgcaaagga
    10681 cggcgccgga ggttgccgcc gatcccgctg ccatcctcaa gggcatcgcc cagcccggct
    10741 cgccggtggc gcaggggctg acgcagttga tggcgaccga ctccagcttc gatccgcgcc
    10801 agttcgtctc cggcgcgcgc gcggcctacg aaatgatcgt gcttgccttt gcccgtggcg
    10861 accgcaaggc gctcaaggac cttttgtcga aggaggtctt cgacaacttc gtcgcggcca
    10921 tcgccggacg cgagcagcgc ggcgaaacgg ttgagacgac cttcgtttcg atcgatcgtt
    10981 ccgaggttgt cgaggcggag ctgaagggca actccatgca ggtgacggtg aaattcgcgt
    11041 ccaagctgat caccgccacc cggaatgcgt ccggagcgct gatcgagggc tcggccgaac
    11101 ggattgccga tctggtcgat gtctggacgt tcgcccgcga cgccaattcg cgcgatccga
    11161 actggcgtct gatctcgacc gaagcggacg agtgattgcc cgcgcggccg gcgttgtcct
    11221 ggcgatggtg ctgaccggca tgccagcgct ggcgaggccg cccctgccg
//
LOCUS       DAOMXN010000058        11209 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_3129_length_11209_cov_12.950780, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000058 DAOMXN010000000
VERSION     DAOMXN010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 11209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11209
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3129_length_11209_cov_12.950780"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1108
                     /locus_tag="PK812_06205"
     CDS             <1..1108
                     /locus_tag="PK812_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 44 protein"
                     /protein_id="HRJ69184.1"
                     /translation="RWGGRTPAAAAPDLGVADVPHLLARLIERYGGAGSERGIHAYAL
                     DNEPGIWFQTHPRLMPRRITIRAFIERSVAAARVIKSLDPRARVFGPGSWGATEMVSF
                     QDAPDWPEYRRQGSFLAAYLAAFRAASEDAGLRLLDVLDVHWYPFSRHGNLFRTENAA
                     LDAALLDAPRGLTEAGYREDSWVADALRVAAPDRPGLPILPSLRRLIDRRFPGTDLAI
                     TEFNYGGAGRLAAGLALADVLGRYGAANVAYATHWGSLDGWLKEAYRLFRMPDASGRR
                     FGGRHLPVQGQTPELDVHAAADEGAVQLIAINKRTEPVAMDVAFGAGRAARLLGVLGF
                     DALHPTTAPVTDASAGEGGRLILPGRSARRYTFA"
     gene            complement(1093..2094)
                     /locus_tag="PK812_06210"
     CDS             complement(1093..2094)
                     /locus_tag="PK812_06210"
                     /inference="COORDINATES: protein motif:HMM:NF013255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRJ69185.1"
                     /translation="MTPPAPATSRTRKPVVLITGAGGFIGTALAERLVEKGILVRSGV
                     RSAPARHGSQVPCDLDRPEQIATAVQGVDLVVHAAYGSAGDMEAQCRSLLDAMAIADV
                     RRLVCLSSIAVYGDATGDIAEDFSQPAERDGYGLAKQACETAVRAFAGQSGRSAVILR
                     PGIVYGAGSPFWIDKLKDRIESGVWGRFGEDGEGIAALIHVDDLAEQIAAAVGRLMDP
                     VGAPTGAVALNAIGPQTPSWNTYFTALARHLGHDLRDLSAGERKLRQAVAVAAKLWRK
                     LGLPGGRAAALFPMPAEMALFARKARYCTNQARDFLGVEPHIGLAEGLARSLPQAKV"
     gene            complement(2091..3557)
                     /locus_tag="PK812_06215"
     CDS             complement(2091..3557)
                     /locus_tag="PK812_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRJ69186.1"
                     /translation="MAERGKATGRLLGWLGWAGTDAIGRLLLMTGSTIVFSRLLAPRE
                     FGIAALVLTIVAAAALFVGSPFEEALAQRRHVRRQHFRAALGVSWLVAGVIVVLATLL
                     GAPLAALYTEPEVGLLLPLAMTSVFFSGHSDILSALARRLRRFNDIAIATLAGHVVGI
                     AAALAMAFAGFGLLALIGQRLFVVVARAIILQWRLGFLILPAWAPGHVRSFGRFAGYS
                     FLSRLVENITYLAFTNLVQALYGIAVLGYVNMAMRLIEPIRGAIVATAHNVAFSFFAR
                     AAHDGEKLKRIASETASQAALAIAPIFTGLAAVSPVLLPLVAGPGWEDAIGIAVCLSL
                     AAALAVPAGLIYTAFSATGRPEYSLYSLVVGFALISAILVAAKALGPISVGYSRVGGD
                     LVRALFAILLSAATLPWTLGARLRTLAPAWLLSGIMGIAVAALGHALAGIAPVARLMI
                     MISLGILIYAALLMVFARPFIIGLAARLRGGPHSGTSA"
     gene            complement(3550..5046)
                     /locus_tag="PK812_06220"
     CDS             complement(3550..5046)
                     /locus_tag="PK812_06220"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GumC family protein"
                     /protein_id="HRJ69187.1"
                     /translation="MSRQKRPAAPRNTGWKANAYSSPWWDTFEILHAAVWRRMLRIIM
                     VTITLATLGAAGRFLVPPSYVATSQVLFDPRGLKVFSNELTAGYYDANSAISFVESQM
                     GVLQSERVHSRIVTMLCDGSSLPQGVARPSFCPAAGSTRAMDSAVNTIKRSITVRRSE
                     RSFLVDINATSSDPQLSAAIASATVAAYQAEDAATRTDVANRLTADLGARSTNLRKAL
                     KDSEERADQYRNDRNLIRVGDRLLVEQRLAAATTALNESQTRLDRIGARARQIDLAPR
                     RSGYLAVLGSEPETRALQGLLERRNSFLVEMAPLAARAGDRHPAMIELRSRLSEINRS
                     IAIEVAQIRESSHADLSRAQTEQDSLAATVADLSVKVAQARRAEAELKVLDQETESNR
                     KLLESFETRAREAREFSKVDVGNLRIVSVARAPETRNMVPHIIAWGFLGAILGLILSI
                     ATTMIAAGIGMAHASYKRRKDYREAVEKANPVYSMRMKAEAFARYRYG"
     gene            complement(5314..6753)
                     /locus_tag="PK812_06225"
     CDS             complement(5314..6753)
                     /locus_tag="PK812_06225"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HRJ69188.1"
                     /translation="MSLDFGSAAAHTPFAANAKLLARLSFSLVAVLVDVAAIMAAAFA
                     ADVGYNFLMHGNFAVCAANIQLGMFAAILFAIANMARQGYKVSAFMDVPEQAQKAFSL
                     WTMSFLAAATFGFLAKAIEDSSRGAFLAMFFIGISCLFAARAALTLFVRRHATNDGIL
                     AARIMIVGFREDVAAFMSQHNLPRQGMTVLGTKVLSDSSAMTDVELSEVTGVARRLVP
                     DDIFLAVPWDRADMIERCTNAFLRVPSSLHLVMDPHSALSRFAVTQMTTRGSISSLRL
                     RGYAMSSVGMALKRLFDVLVSLLALIVLLPLFLVVAIAIKLESRGPVLFFQTRHGFNK
                     MPFRIMKFRSMRTMEDGQNVRQVTANDPRITRVGRLLRKYNIDELPQLFNVLRGEMSL
                     VGPRPHALVHDQQFEPEVVLYARRHNVKPGITGWAQVSGYRGETDTSEKISKRVQYDL
                     YYIDHWSFVFDVWILFLTIFSRRAYANAA"
     gene            complement(7087..8505)
                     /locus_tag="PK812_06230"
     CDS             complement(7087..8505)
                     /locus_tag="PK812_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007010872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HRJ69189.1"
                     /translation="MSGSLKFGTSGLRGLVTELCGAPAYAYTRAYCAMLADDGSPRGS
                     GMLIARDLRASSPDIAELCIQAIADSGFTPLDCGAVPTPALALLALREGLPAVMVTGS
                     HIPEDRNGLKFYRATGEIDKADEQRILAHHARLAIMQAPPPSARAERRDALSAYATRY
                     AEFFPPDGLAGLRVGIYQHSSVARDMIADILSGMGATVLPFARAGSFIPVDTEAIRLE
                     DRELARAFAAENRVDAIVSTDGDADRPLVADEAGAFLRGDLIGALTAASLGADAIVTP
                     VTSNSALEACGKFASIRRTRVGSPFVLEGMAEAAASGARVVVGFEANGGVLLGSDVAV
                     AGRRLAALPTRDAMLPILAILREIAHTRRPLSDLVRSYSFKIALSDRIPHVAAEKSLP
                     VMARLERDDPFADATFAPWGGIAGRDTRDGLRMSTGAGDIVHLRQSGNAPELRVYVEA
                     ADEVRAGALLEFALTAARTLVA"
     gene            complement(8515..9939)
                     /locus_tag="PK812_06235"
     CDS             complement(8515..9939)
                     /locus_tag="PK812_06235"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HRJ69190.1"
                     /translation="MPGVRKMIPIIMCGGAGTRLWPVSRDSMPKQFVRLFGEESTFQR
                     TVRLLSDRSVFEAPIVLTATDYRFTVEDQLKAIGIDCSIVLEPQRRDSAAAVAVATLL
                     SLQRDPDAIVGVFAADHVVQDGAGFVATCALAARTAAAGRIVTIGIRPTHPATGYGYI
                     KPGAPTEPGSEARIVEAFVEKPDAARAERYVLDGYLWNSGNFIFHGATMASELEKHAA
                     DVLHPAADAVATAKRDLAFTVLGEEAFSAARKIAIDFAVMEKTTQAAVVPGSFGWSDV
                     GGWAAVWDLSDKDEDGNAIIGKGHVLEGRNNLVRSEDGVVGVVGLSDIAVVSTRDATL
                     VVPRDRADRVKELVEQLRLRGEPAASAHREIHRPWGKYVSIDSGARHQVKRITVKPGG
                     VLSLQKHFHRAEHWVVVRGTAEVTREKETIIVHENESIYLPIGCVHRMKNPGKIDLDI
                     IEVQVGSYFGEDDIVRLEDVYRRT"
     gene            10209..>11209
                     /locus_tag="PK812_06240"
     CDS             10209..>11209
                     /locus_tag="PK812_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HRJ69191.1"
                     /translation="MTEHEQAGGPLKGLRVVEMGGIGPGPLAGQLLADLGADVILVER
                     KSGPANRFDINARGKRSVALNLKSAEGVEACLKLIGRADALIEGFRPGVMEKLGLGPD
                     VCLSRNPRLIYGRMTGWGQDGPLAQTAGHDINYLSITGALHAMGRANEPPMPPLNLVA
                     DYGGGAMFLAFGILAALFERQTSGRGQVIDAAMVDGVPALMGLIHGFVASGFWTRKRE
                     ANWLDGGAPFYGCYETADGKYLSVGPLEPQFFAELVDRAGLPQSHRASQNDRATWEER
                     RRDYAALFRMRTRDAWMALFEGTDACVAPVLGWDEVEQHPHNAARGNFLRVDGVLQSA
                     "
BASE COUNT         1981 a         3550 c         3862 g         1816 t
ORIGIN      
        1 gcggtggggc gggcgcacgc ctgccgccgc cgccccggac ctcggggtcg cggacgttcc
       61 gcatttgctt gcccgcctca tcgaacgcta cggcggagcg ggatcggagc gcggtatcca
      121 tgcctatgcg ctcgacaatg agcccggcat ctggttccag acccatccgc gacttatgcc
      181 tcgccgcatc accattcgcg cgttcatcga gcggtcagtc gcggcggcac gggtgatcaa
      241 gtccctcgat ccccgggcgc gggtgttcgg ccccggcagt tggggcgcga ccgagatggt
      301 cagcttccag gatgcgccgg actggccgga gtaccggcgc cagggcagct tcctggcggc
      361 ctatctcgcc gccttccggg cggcttcgga agacgcgggc ttgcgcctgc tcgatgttct
      421 ggatgtgcac tggtacccgt tcagccggca cggcaacttg tttcgcacag agaatgccgc
      481 gctcgatgcc gcgctgctcg atgcgccgcg cggcttgacg gaagccggct atcgagaaga
      541 cagctgggtt gccgatgcgt tgcgcgtcgc cgctccggac cggcctggcc tgccgatcct
      601 gccgagcctg cgtaggctga tagatcggcg ttttccgggc acggatcttg ccatcaccga
      661 attcaactac ggtggcgccg gtcgtctggc ggcgggtctt gcactcgccg atgttctggg
      721 tcggtacggc gcggccaacg tcgcctacgc cacccattgg ggcagcctcg acggctggct
      781 gaaggaggcc tatcggctgt tccggatgcc ggatgcgtcc ggtcggaggt ttggcgggcg
      841 tcatctgccg gtgcaagggc agacgccgga gctcgacgtc catgcggcgg ccgacgaggg
      901 tgcggttcag ctcatcgcga tcaacaagcg cacggagccg gtggccatgg acgtcgcttt
      961 cggcgccggc cgggccgcga ggctgctggg ggtcttgggc ttcgacgcgc tgcacccgac
     1021 cacggcgccc gttacggacg cgagtgcggg cgaaggcgga agactgatcc tgcccggccg
     1081 gtccgcccgc cgctacacct ttgcctgagg cagggagcgc gccaaacctt cggcgagacc
     1141 gatgtgcggt tcgacgccga ggaagtcccg ggcctgattt gtgcagtagc gggcctttcg
     1201 cgcgaacagg gccatttcag cgggcattgg gaagagggcg gcggcgcgtc ctcccggcag
     1261 gccgagcttg cgccagagct tcgcggcgac ggcgacggcc tgccttagct tgcgttcccc
     1321 cgccgacagg tcgcgcaggt catggccgag atgccgggcg agcgcggtga aataggtgtt
     1381 ccagctcggc gtctgcggac cgatggcgtt gagcgcgacc gcgccggtcg gagcgccgac
     1441 ggggtccatg agccggccca cggcggcggc aatctgctcg gcgagatcgt cgacgtggat
     1501 cagtgccgct atgccctcgc cgtcctcgcc gaagcggccc cagacgccgc tctcgatgcg
     1561 gtccttcagc ttgtcgatcc agaacgggct gccggcgccg tagacgatgc ccggccgcag
     1621 gatgacggcg gagcggcctg actgtccggc aaaggcacgc acggctgtct cgcaggcctg
     1681 cttggccagg ccatagccat cacgctcggc cggctgtgaa aagtcctcgg cgatgtctcc
     1741 cgtggcgtct ccgtagacgg cgatggaact gaggcagacg aggcgacgaa cgtcggcaat
     1801 cgccatcgcg tccagcagcg atcggcattg cgcttccatg tcgccggcgc tgccgtaggc
     1861 ggcatgcacg accagatcca cgccttgcac ggccgtcgcg atctgctccg gccggtcgag
     1921 gtcgcagggg acctgcgaac cgtgccgagc aggggccgaa cgcacgccgg accgcacgag
     1981 gattcctttt tctacgagcc tttcggcgag cgccgtgccg ataaaaccgc cggcgccggt
     2041 gatgaggacc acgggtttgc gcgtacgtga agtcgcaggg gcagggggcg tcatgcgctg
     2101 gtcccggagt gcggcccgcc gcgaaggcgc gcggcgaggc ctatgatgaa cgggcgggcg
     2161 aaaaccatca gcagcgcggc atagatcaga attccaagcg aaatcatgat catgagacgc
     2221 gcaaccgggg caattcctgc gagggcgtga ccgagcgctg caaccgcgat ccccatgatg
     2281 cctgagagca gccaggctgg cgccagggtg cgcagtctgg cgccgagggt ccagggcagg
     2341 gtggctgcgc tgagcaggat cgcaaacagc gcccgtacca ggtcgcctcc gacacgcgaa
     2401 tagcccacgc tgatcgggcc gagtgccttt gcggcaacaa ggatggcaga gatgagcgcg
     2461 aagccgacta ccagcgagta gagactgtat tccggccgtc cggttgcgga aaaggcggtg
     2521 tagatcaggc cggcgggaac agcgagcgcg gcggcgaggg acaggcagac ggcgatgccg
     2581 atcgcgtcct cccagcccgg accggcgacc agcggcagca gcacgggaga aaccgcggca
     2641 aggccagtga agatcggcgc gatggccagc gctgcctgag acgcggtctc ggaagcgatg
     2701 cgtttcagct tttccccatc atgggccgct cgggcgaaaa aggagaaggc gacgttgtgc
     2761 gccgtggcga cgatcgcacc gcggatgggc tctatcaggc gcatcgccat gttgacgtag
     2821 ccaagaacag cgatgccgta gagagcctga acgaggttgg tgaaagcgag gtaggtgatg
     2881 ttctcgacaa gccgggacag aaaagagtag ccggcaaagc gtccgaacga gcgcacgtgt
     2941 cccggcgccc acgccggcag gatcaggaag cccagccgcc attgcaggat gatggcgcgg
     3001 gccacgacga cgaacagccg ctgcccgatg agcgcaagca ggccaaaccc ggcaaaggcc
     3061 atcgccagcg cggccgcgat gccgacgacg tgaccggcca gcgtggcaat ggcgatgtcg
     3121 ttgaaccggc gcagccggcg cgccagggcc gaaaggatat ccgagtggcc actgaagaag
     3181 acggaggtca tggccagcgg cagcagcagg ccgacctcgg gctccgtgta cagcgccgca
     3241 aggggcgcgc cgagcagcgt cgccagcacg acgatgacgc cggcgacgag ccaagaaacg
     3301 ccgagagcgg cgcggaaatg ctgacggcgg acatgacgcc gttgcgccag ggcctcctcg
     3361 aacggcgagc cgacgaacag ggcggcggcg gcgacgatgg taagaaccag ggcagcaatg
     3421 ccaaactctc gcggcgccag cagccgcgag aagacgatgg tgctgccggt catcagcagc
     3481 agacggccga tggcatccgt tccggcccat cccagccagc ccagcagccg gccagtagct
     3541 tttcctcgct cagccatagc ggtagcgggc gaaagcctcc gccttcatgc gcatggaata
     3601 gaccgggttg gccttctcca cagcctcgcg ataatccttg cggcgcttgt aggaagcatg
     3661 ggccatgccg attccggcgg cgatcatggt cgttgcgatc gacaggatca ggcccaggat
     3721 ggcgccgagg aaaccccagg cgatgatgtg cgggaccatg ttccgcgtct ccggcgcgcg
     3781 ggcgacagag acgatgcgca ggttgccgac atcgactttc gagaactcgc gcgcctcgcg
     3841 tgcgcgcgtt tcgaaggatt cgagcagctt gcggttcgac tcggtctcct ggtcgagcac
     3901 cttgagttcg gcttccgccc ggcgcgcctg cgcgaccttc accgacaggt cggcgacggt
     3961 ggccgcgaga ctatcctgct ccgtctgtgc ccgcgacagg tcggcatgtg agctttcacg
     4021 gatctgcgcc acctcgatgg cgatgctgcg gttgatttcg gaaaggcgcg aacgcagctc
     4081 gatcatggcg ggatggcggt ctccggcgcg agcggccagc ggggccatct cgaccaggaa
     4141 actgtttctg cgctccagca atccctggag cgcccgtgtt tccggctcgg agccgaggac
     4201 ggcgagatac cctgatcttc gcggcgcgag atcgatctgg cgggcacgcg cgcctatgcg
     4261 atccagccgc gtctgcgatt cgttcagcgc ggtcgtggcc gccgcgaggc gttgctcgac
     4321 gagcaagcgg tcgcctaccc ggatgaggtt gcgatcgttg cggtactggt ccgcgcgttc
     4381 ctccgagtcc ttcagcgcct tgcgcagatt cgtggacctc gcgccgaggt cggcggtgag
     4441 ccggttggcg acgtcggtgc gggtcgccgc atcttcggcc tgataggcgg ccacggtcgc
     4501 ggaagcgatg gcggcagaca gctgcggatc ggacgacgtc gcgttgatgt cgacaaggaa
     4561 ggaccgctcg gatcgccgga ccgtgatgga gcgcttgatg gtgttgacgg cgctgtccat
     4621 tgccctggtg ctgccggcgg ctggacagaa ggacggcctg gctacgccct gtggcaggga
     4681 cgaaccgtcg caaagcatgg tcacgatgcg cgagtgaacg cgttccgact gcaagacgcc
     4741 catctggctc tcgacaaagc tgatggcgga gttcgcgtca tagtagccgg cggtcagctc
     4801 gttggagaat accttgaggc cgcgcgggtc gaaaagaacc tgggaggttg cgacatagct
     4861 cggcggaaca aggaagcgcc ccgccgcgcc gagggtggcg agcgtgatgg tcaccatgat
     4921 gattcgcagc atcctgcgcc agacggcggc atgcaggatt tcgaacgtgt cccaccacgg
     4981 gctggaatag gcgttcgcct tccaacctgt attgcgcggt gctgctggtc gcttttgtct
     5041 ggacacggtc ggcctgatag ttcgctgcag aagctaccgg ccaaaaacga ttcaattcat
     5101 aatgatcgtg gaaatgatgc cctatggtta tgcgaatcta aaacgttgaa tcctcagcgc
     5161 ttgtcgtttt gagtcagggc gtggacggac cggcgtgccg gtggcgcgcc ggacggtctc
     5221 ccgacggtgc tggaacctgt ggatcgtgct gaaaactcag gcgatttttg ggtggaaacc
     5281 cgggtctcgc gcgcggttca gcgcccattt tccctacgcc gcgttggcgt aggctcgccg
     5341 cgagaagatc gtcaggaaca ggatccagac gtcgaaaacg aacgaccagt ggtcgatgta
     5401 gtacaggtcg tactgcacgc gcttggaaat cttttccgag gtatcggtct cgccgcgata
     5461 accactgacc tgcgcccaac ccgtgatgcc cggcttcaca ttgtggcgac gcgcgtaaag
     5521 caccacttcg ggctcgaact gctggtcgtg gacgagcgca tgcggccgag gcccgaccag
     5581 cgacatctcg ccgcgcagca cgttaaagag ctggggaagc tcatcgatgt tgtacttgcg
     5641 caggaggcgg ccgacccgcg tgatccgagg atcgttggcg gtcacctgac gcacgttctg
     5701 cccatcttcc atggtccgca tcgagcggaa cttcatgatc cggaacggca tcttgttgaa
     5761 accgtgccgg gtctggaaga acagcaccgg cccgcggctt tccagcttga tcgcgatggc
     5821 cacgaccagg aacaacggca ggaggacgat cagcgccaga agcgaaacaa ggacatcgaa
     5881 cagccgcttc agcgccatgc cgacactgct catggcatat ccgcgcaggc gcaggctcga
     5941 aatcgatccg cgcgtcgtca tctgcgtgac ggcgaagcgg ctgagagcag aatgcgggtc
     6001 catcaccaga tgaagcgagg agggcacgcg caggaaggcg ttggtgcacc gctcgatcat
     6061 gtcggcccgg tcccacggca cggcgaggaa gatgtcgtcc ggcacgagac gacgcgcaac
     6121 gcccgtcact tcggacagtt cgacgtcggt catcgcgctc gagtccgaca ggaccttcgt
     6181 tcccaggacc gtcatgccct gtctggggag gttgtgctgg ctcatgaagg cggcgacatc
     6241 ctcgcggaat ccgacgatca tgatccgggc tgcaaggatg ccgtcgttcg ttgcgtgtcg
     6301 gcgcacgaac agtgtcaacg cggcacgggc ggcaaacagg cagctgatgc cgatgaagaa
     6361 catggcgagg aaggcgccgc gcgaggagtc ctcgatggcc ttggccagaa atccgaaggt
     6421 cgcggccgcc aggaacgaca tggtccagag gctgaatgcc ttctgcgcct gttcgggcac
     6481 gtccatgaag gcggacacct tgtagccctg ccgcgccatg ttggcgatgg caaaaaggat
     6541 ggccgcgaac atgccaagct ggatgttcgc ggcgcagacc gcgaaattcc cgtgcatgag
     6601 aaagttgtac ccgacgtcgg cggcgaaggc ggccgccatg atcgcggcaa cgtccaccag
     6661 cacagccacc agggaaaagc tcagccgtgc cagcagcttg gcattcgcgg caaagggcgt
     6721 gtgcgcggcg gcggatccga aatccagcga cacgctcgct gtccggactg catcatacgc
     6781 cgcgacgtcg cgcggtcgga agtgaccacc gtctgtcata acgatccctg gcgtcgcgcc
     6841 gaccgtttgc aaacggttct gtcacaacga acgcagggga aagctacagc gcgaacatga
     6901 agacaaacat aaatgacata atgaaaatgc cgtttacctc gtaactctga ggcatggtga
     6961 agaaaaagcg tttcaataca gtcgctgttg ccttaattgt gaatatcttc gtccatcggc
     7021 tcgcgggcca ggttggctgc gcagcggacg aacgctgcgt caggcggggc ggcggtccga
     7081 agttacctaa gcgaccaacg tgcgcgcggc cgtcagggcg aattccagca gcgcgccggc
     7141 gcgcacctca tctgcggcct cgacatagac ccgcagttcc ggcgcattgc cggactggcg
     7201 cagatgcacg atgtcgcccg cgccggtgga catgcgcagg ccatcgcgtg tgtcgcggcc
     7261 agcgattccg ccccacggtg cgaaggtggc gtccgcgaac gggtcatcgc gttccagccg
     7321 ggccatgacc ggcaggcttt tctcggccgc cacatgcggg atgcggtccg agagcgcgat
     7381 cttgaagctg taggagcgga ccagatcgga gaggggccgg cgcgtgtgcg cgatctcgcg
     7441 caggatggcg aggattggca gcatggcgtc gcgggtcggc agcgcggcga ggcgccgacc
     7501 ggcaacagcg acatccgagc ccagcagcac gccgccattg gcttcgaacc cgaccaccac
     7561 gcgtgcgccg gatgccgccg cttccgccat cccttccagc acgaagggcg agccgaccct
     7621 ggtgcggcgt atggaggcga attttccgca ggcttccagc gccgaattgg atgtaaccgg
     7681 cgtcacgatg gcgtcagcac cgagggaagc cgcggtcaag gctccgatca ggtcgccgcg
     7741 caggaaggcg ccagcctcgt ccgcgaccag cgggcgatcc gcgtcgccat cggtcgagac
     7801 gatcgcgtcg acccggttct cggcggcgaa tgcgcgggcg agctcccgat cttccaggcg
     7861 gatcgcttct gtgtcgaccg ggatgaagga gccggccctc gcgaatggca ggaccgtcgc
     7921 gcccatcccg gacaggatgt cggcgatcat gtcgcgcgcc acggaggaat gctgatagat
     7981 gccgacgcgc aagccggcga gaccgtccgg cgggaagaat tccgcatagc gcgtggcgta
     8041 ggccgaaagc gcgtcgcgcc gttcggctct ggccgagggt ggcggggcct gcatgatggc
     8101 gaggcgagca tggtgggcga ggatgcgttg ttcgtccgcc ttgtcgattt cgccggtggc
     8161 ccgatagaac ttcaggccgt tccggtcttc cgggatgtga ctgcccgtca ccatgacggc
     8221 gggcaacccc tcgcgtagcg ccagcagagc caaggccggc gtcggtacgg cgccgcaatc
     8281 gagcggtgtg aagccgctgt cggcaatggc ctggatgcac aactcggcga tgtcggggct
     8341 gctggcgcgc aagtcgcggg cgatcagcat gccgctgcca cgcggggagc catcatccgc
     8401 cagcatggcg cagtaggccc gcgtgtaggc ataggccggg gcgccacaga gctccgtgac
     8461 caggccacgc aggccgcttg tgccgaactt gagggacccg ctcatcacac cggctcaggt
     8521 acggcggtag acatcttcga ggcgcacgat gtcgtcctct ccgaagtagg agccgacctg
     8581 aacctcgatg atgtcgagat cgatctttcc cgggttcttc atccggtgca cgcagccgat
     8641 gggcaggtag atcgactcgt tctcatgcac gatgatcgtt tccttctcgc gcgtcacttc
     8701 cgcggtgccg cgcacgacga cccaatgctc ggcgcggtga aagtgcttct gcaacgaaag
     8761 cacgccgccc ggcttcaccg tgatgcgctt gacctggtgg cgggcgccgg aatcgatgga
     8821 cacatacttg ccccacggcc tgtggatctc gcggtgggcg ctggcggccg gctcgccccg
     8881 cagccggagc tgctcgacaa gctccttgac ccggtccgcg cggtcgcgcg gcacgacgag
     8941 cgtcgcatcg cgcgtcgaga caacggcgat atcgctcagg ccaacgacgc cgacgacccc
     9001 gtcctcggag cgcaccaggt tgttgcggcc ttcgagcaca tggcccttgc cgatgatggc
     9061 gttgccgtcc tcatccttgt ccgacaggtc ccagaccgcc gcccagcctc cgacgtccga
     9121 ccagccgaac gatccgggca cgaccgcggc ctgggtggtc ttttccatga cggcgaaatc
     9181 gatggcgatc ttgcgcgccg ccgaaaaggc ctcttccccg agcacggtga aggccaggtc
     9241 gcgcttcgcc gtcgccacgg cgtctgccgc gggatgcagg acatcggccg cgtgcttttc
     9301 gagctcggaa gccatggtcg cgccgtggaa gatgaaattg ccggaattcc acaggtagcc
     9361 gtccagaacg tagcgttcgg cgcgcgccgc atccggcttc tcgacgaagg cctcgacgat
     9421 ccgggcctcg gagcccggtt ccgtcggagc accaggcttg atgtagccat agcccgtggc
     9481 cgggtgcgtg ggacggatgc cgatggtgac aatgcgcccg gcggcagcgg tgcgcgccgc
     9541 gagcgcgcag gtcgcgacaa agccggcgcc atcctggacg acgtggtccg ccgcgaagac
     9601 gccgacgatg gcatcggggt cgcgttgcag agacaacagc gtcgccacgg ccaccgctgc
     9661 ggccgaatcg cggcgctggg gctccagcac gatcgagcag tcgatgccga tggccttgag
     9721 ctgatcctcg accgtgaatc gatagtcagt ggcagtcaga acgatcggcg cctcgaacac
     9781 ggagcggtcc gacagcagtc tcaccgtgcg ctggaaggtt gattcctccc cgaacaggcg
     9841 cacgaactgc ttgggcatgg aatcgcggga gaccggccag agccgggtgc cggcgccacc
     9901 gcacatgatg atggggatca tcttgcgaac accaggcatg attcaatctc cttgccgcgc
     9961 ccggaatgca ccggaacgct tcccgattcc cgacagtgtg acgccagtgg cgccgccttg
    10021 ccattcaaac atgcgcgtgc gacccggcgg cgcatgcctg cgaagcggtt tatgaccatt
    10081 cgccggccgg agtcgaggcg catgtcgatg cgctcgggag ttttgttgca ctggcgctcg
    10141 tgcgatttcc tgttgccaga gttggttcag atgtaaatca tctctcccgg agcacaggca
    10201 ggcggaccat gactgaacat gagcaagcgg gcggccctct caaaggcttg cgcgtcgtcg
    10261 agatgggggg aatcggcccc gggccgcttg ccggccaatt gctggcggat ctcggcgcgg
    10321 atgtcattct ggtggaacgg aagtccgggc cggccaaccg ctttgacatc aatgcgcgcg
    10381 gaaagcgctc cgtcgcactc aacctcaagt ccgccgaagg cgtcgaagcc tgcctgaagc
    10441 tgatcggccg ggccgatgcc ctgatcgagg ggttccggcc cggcgtcatg gagaagctgg
    10501 ggctggggcc tgatgtctgt ctttcccgca acccgcgcct gatctacggc cgcatgaccg
    10561 gctggggaca ggacggacct ctggcgcaga ccgccggcca cgacatcaac tacctgtcga
    10621 tcaccggggc cctgcatgcg atgggccgcg ccaacgagcc acccatgccg ccgctcaacc
    10681 tggtggccga ctatggcggg ggcgcgatgt tcctcgcctt cggcatcctt gccgcactgt
    10741 tcgagcgcca gacgtcgggc cgggggcagg tgatcgacgc tgcgatggtg gatggcgttc
    10801 ccgcgctcat ggggttgatc cacggattcg tcgccagcgg cttctggacg cggaagcgcg
    10861 aggccaactg gctcgacggc ggcgcgccgt tctatggctg ttacgaaacc gcggatggca
    10921 agtacctgtc cgtcggaccg ctggaaccgc agttcttcgc cgaactcgtg gaccgcgcgg
    10981 ggcttccgca gagccatcgg gcttcccaga atgaccgcgc cacctgggag gagcggcggc
    11041 gcgactatgc cgcgctcttc cgcatgcgaa cgcgcgacgc gtggatggcg ctgttcgagg
    11101 gtaccgacgc gtgcgtggcg ccggtgcttg gctgggacga ggtggagcag catccgcaca
    11161 atgcggcgcg gggcaacttc ctgcgcgtcg acggcgtcct gcagtccgc
//
LOCUS       DAOMXN010000059        11177 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_3140_length_11177_cov_11.571210, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000059 DAOMXN010000000
VERSION     DAOMXN010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 11177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11177
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3140_length_11177_cov_11.571210"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="PK812_06245"
     CDS             complement(<1..842)
                     /locus_tag="PK812_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HRJ69192.1"
                     /translation="MISLDDIRAAADRVRPHVRRTPVLDVEADALGVDRPLSLKLEFL
                     QVTGTFKARGAFNALLSAPLPAAGVCAASGGNHGFAVAHAARRLGVPARIFVPEISSP
                     AKITRIRETGVELVVGGARYADALTACEAYAARTAARSIHAYDAWPTIAGQGTVGLEW
                     QEQTPGLDTVLVAAGGGGLVSGIARWFESRVRVVAVEPEGSCALHRALAAGAPVDVPV
                     QSVAADSLGAANVFSRVHQTCARYLDHVALVPDSAILEAQRRLWLGLRVAVEPGGAAA
                     LAALV"
     gene            complement(1196..2080)
                     /locus_tag="PK812_06250"
     CDS             complement(1196..2080)
                     /locus_tag="PK812_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRJ69193.1"
                     /translation="MKAYLNTEEAAAYLGIKERKLYELVANGAVPCSKVTGKWLFPQA
                     ALDRWVEANLAHPGGFSPLAPPPIIGGSHDPLLEWAARRSGSGLALLSEGSSAGLAKL
                     DRNEVAMAAIHLHAVGSDADVNIAGVQACPKLTDAVVVAFARREQGLLTATGNPLKLV
                     TLADVASREARIGFRQSGAGAQLFLEVLAAAARLPLDALNRKGDPFTTGHDLAFAIRA
                     GEIDCGVATRADAAANSLGFVPLAWEHFDLVMRRRTYFEPGPQRLFALLREPEFRRHA
                     DMLTGYDISDTGSIRFNR"
     gene            2215..2877
                     /locus_tag="PK812_06255"
     CDS             2215..2877
                     /locus_tag="PK812_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ69194.1"
                     /translation="MVGLDPQFLQVVGLSLRVTLTAVLLAALIGVPLGAWLALARFPG
                     RSAVIVLVNGLMGLPPVVAGLIVFLALSRSGPLGGLGWLFTPTAMVIAQTLLVLPIVL
                     ALTRSTLEELWGEYRDHLTSLGMTGLRAIPTLIFDGRFALATGLLAGFGRASAEVGAV
                     LIVGGNIAGYTRTMTTSIALETSKGDLPLALGLGIVLIALTLAINAAAYGASRVGARF
                     AG"
     gene            2883..3605
                     /locus_tag="PK812_06260"
     CDS             2883..3605
                     /locus_tag="PK812_06260"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRJ69195.1"
                     /translation="MRDTPSILPLSVQRLCFETDGKRLLEDVSFTLPGGGVTTIIGPN
                     GAGKSLTLRLCHGLLTPTSGTVHWGAAPGAAVGRKRHAMVFQRPIMLRRSVRANLVHA
                     LSAVGAADAALRVTAALERFGLNDIAERPARLLSGGEQQRLAIARAWSLSPEVLFLDE
                     PTSQLDPGATRQIEGIIASLASEGMTILMTTHDLGQARRLGVRILLLNKGRLIEDAAT
                     ARFFAAPGTREGQAFIAGELLW"
     gene            3640..4512
                     /locus_tag="PK812_06265"
     CDS             3640..4512
                     /locus_tag="PK812_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015823156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRJ69196.1"
                     /translation="MVTKRNLITAGFSLAFTGLALAQTAQPAAPAQSGAAAGTRFITV
                     SSTTSTTDSGLFNHLLPAFKAKTGIEVRVVSQGTGQALDTGRRGDADVVFVHARAQEV
                     KFVEDGFGVERKPVMYNDFVLIGPKQDPAGIKGSRDIAAALKAIQAKATPFVSRGDKS
                     GTHSAELNLWKVAEIDIEKTKGPWYREIGQGMGAALNTSGAMGAYVLSDRATWLNFRN
                     RGELEIVVEGDRRLYNQYGVILVNPARHAHVKKADGQAFIDWLTGPEGQKAIADYKIN
                     GEQLFFANATQPGA"
     gene            complement(4611..4688)
                     /locus_tag="PK812_06270"
     tRNA            complement(4611..4688)
                     /locus_tag="PK812_06270"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4651..4653),aa:Pro,seq:ggg)
     gene            4828..6045
                     /locus_tag="PK812_06275"
     CDS             4828..6045
                     /locus_tag="PK812_06275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRJ69197.1"
                     /translation="MHVVIILDQAHVNGGQAKVALDSAFGLKARGHTPVVFAASGPVM
                     PELEAAGIETVCLGQHEFVTDPSKAAAALRGLWNFEARKALATLLARLPQGDTVVHLH
                     GYAKALSSSVVPAIRAAGLPVLYTMHEYYLLCPNGGFYNYRENHHCTLKPLSPACWKT
                     HCDTRSYGFKLWRGARGVISSRIADVFGTLTDIAHFHGFQKQIIAPHLPPATRFHEIA
                     NPIEAADLGPKSTPERGDFVFVGRLSTEKGCPLFAEAAARAGVVPAFVGDGPAAEDLK
                     RRYPQARFHGWQDIAGVRRALREARCLVFPSLWYEGQPLTVLESLAMGTPVIAGDGSA
                     GRESVVDGVTGLWFRQADVNDLAQKITMMRDDARVAAMSRAAYGRYWADPFTLERHVS
                     RLEQVYGRLLTGQ"
     gene            complement(6039..7562)
                     /locus_tag="PK812_06280"
     CDS             complement(6039..7562)
                     /locus_tag="PK812_06280"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HRJ69198.1"
                     /translation="MLAEGQGGLALETAGIGAKGTGAVRLRPAEAFSPMPLGRGRISP
                     IVISGAVRILEFIVVCLTGAAIAWVYLPVEVSARPYWVALPGAAALTLVAFRLLDIDQ
                     PGALRNFVGSAWKVVAGFALVFLVLFALVFFLKFEGAFSRVVFAAWFVFGLAALLALR
                     FVVHLAVKAMTARGLLDRRAAIVGGGATAEALLEALTRDKDSDLRIVGLFDDRTDGRS
                     PDLVAGYPKLGGIDELIGYARNARLDTIIFALPVTAEARILDMLKRVCVLPVDIRLAA
                     HTQQLRFKPRNYSYLGSMPVFDMADRPVADWGLVWKAAFDRVAGALLLLALTPLMLLI
                     ALAVKLDSRGPVLFRQKRYGFNNQMIEVFKFRSMYVEQTDATAARLVTRDDPRVTRVG
                     RFLRKASLDELPQLFNVVFRGDLSLVGPRPHAVHAKAEDRLYDEVVEGYFARHKVKPG
                     ITGWAQVNGWRGETDTHEKIQKRVEHDLFYIENWSLAFDFYILLLTPFALIKSENAY"
     gene            complement(7633..8766)
                     /locus_tag="PK812_06285"
     CDS             complement(7633..8766)
                     /locus_tag="PK812_06285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRJ69199.1"
                     /translation="MSALRVLLVFRAPLGGLFRQVIDLARYLIADGHSVGIVADTIAD
                     NPRAEATFAELKDQCALGIHRLPMHRNPHWTDIGNIRRIRAIAKETQAHVLHGHGSKG
                     GLYARADAALGMGGPVRVYTPHGGSFNYQPGTMAHRVYMVIETLLERGTDVFAFESEF
                     IAGRFRAEVGQTRKIVRIALNGLHPHEFTPRQVAADATDIVFIGELRPVKGIDLLIHA
                     ICRIKKQGRSLTATIIGSGPDEAELKALAEREGIAGQLQFTGPMPAQVGLTRGRIMVA
                     PSRFESLPYIVLESVAAQIPLITTSCGGIPEIVGSDYPWMLPVDDLDRLVGAIMEADA
                     MSDDALRIRAAALADRIRPLFDAQRMGRDIVAAYRAGLAARQG"
     gene            complement(8794..9285)
                     /locus_tag="PK812_06290"
     CDS             complement(8794..9285)
                     /locus_tag="PK812_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HRJ69200.1"
                     /translation="MPPDVLQASLSQPYTLNSGDKLRVIVFGQDALSNVYAVDGAGRI
                     SLPLIGTVEARGLTTAGLERSIEGRLRAGFLREPKVSVEVDTYRPFFILGEVTTSGQY
                     PYVNGMTIQTAVAIAGGFSARAARHYAEVTRVVNGDLLTVNVPITTPIRPGDTILVRE
                     RFF"
     gene            9504..>11177
                     /locus_tag="PK812_06295"
     CDS             9504..>11177
                     /locus_tag="PK812_06295"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GumC family protein"
                     /protein_id="HRJ69201.1"
                     /translation="MRGPIQPDFGQAPAADASLGGVFAALNRRKWWILMATTMAFVAT
                     AAFVNIVTPSYTGEARLLLENRDNYFTRPDRDSRGAEPSIDAEAVQSQVQLILSRDIA
                     RAVIKKLDLSSKEEFDSARREASSLKRLLAIAGLAKDPLASSPEERVLEAYMDKLLVV
                     TVGKSRVISVSFSSKDPDLAARAANTVADEYIRFQGDAKKSTTQGASAWLSQTIETLR
                     AKVAEAEAKVENYRAANGLLIGSSNATITTQQLGEMNTQISNARSAQSDLQAKARLIR
                     EALKNGRIFETSDVVNNELIRRLLEQRVAMKTQIALEERTLLPGHPRMKELTAQLNDL
                     EGQIRTAAERTARTLENDARVAGARMQSLQAEFEGQKRTAADANGSEVQLRALEREAR
                     ALRDNYESYLAKYRDSLSRDSDAASPADARIFSRAIVPQKPAFPKKLPMILLATLATL
                     ILSCAMVVTRQVFMTDPAGEIMPPAMVDPHGRFVAPEAPRHHPGQFQASPPVADPSFN
                     PQPAWHPAPPAAPPAAARVREPQVQAAAPPPVAEPPVLAPEQAAERLQAT"
BASE COUNT         1964 a         3634 c         3673 g         1906 t
ORIGIN      
        1 acgagtgcgg caagggcggc ggcgccgccg ggctcgaccg ccacgcgcaa tccaagccag
       61 agacggcgct gtgcttcgag aatggcgctg tccggcacca gcgccacgtg gtcgagatat
      121 cgcgcgcagg tctggtgcac gcgggaaaac acgttcgccg cacccagtga gtccgccgcc
      181 acggattgca ccgggacgtc caccggcgcg ccggccgcca gggcgcgatg aagggcgcag
      241 gagccttccg gctcgaccgc cacgaccctg acgcggctct cgaaccagcg cgcgatgccg
      301 gacaccagcc cgccaccgcc ggccgcgacc aggacagtgt cgaggccggg tgtctgttcc
      361 tgccattcca gtcccaccgt gccctgtccg gcgatggttg gccaggcgtc ataggcgtgg
      421 atcgagcgcg ccgccgtgcg cgcggcatag gcctcgcagg ccgtcagcgc gtcggcgtag
      481 cgtgctccgc cgaccaccaa ctcgactccc gtctcgcgaa ttcgggtgat cttggccgga
      541 gaggagattt ccggcacgaa gatgcgtgcc ggaacgccca gccgacgcgc cgcatgcgcc
      601 acggcgaatc cgtgattgcc gccagaggcg gcgcagactc ccgccgcggg caggggggcg
      661 gacagcaagg cgttgaaggc gccgcgcgcc ttgaaggtgc ccgtgacctg tagaaactcg
      721 agcttgagcg acagcggccg gtcaacgccc agcgcgtccg cctccacatc gagaaccggt
      781 gtgcgccgca cgtgcgggcg gacgcggtcc gcggcggcgc gaatgtcgtc gagggagatc
      841 atctgtgttc ccggcctgag agtgaacctg ttggagcggt gggccatccc cagaacgccg
      901 atgtccaaag catgcgtgtc ttcggaaaga tcaaacgtgt gacgatcaat tcggaggctt
      961 gccactgcaa ttcatgggtg tttcgaagcc agtgtcaacc gccaggcatg gcgtcaccgg
     1021 cgcccggcat gggcttcacc gatggcgtgc gcatgggccc tcgtatcggt gttgccggac
     1081 gccctcggga ggccgagcgg accggccgcc accaggcgcg accaagggtg aggccatcgc
     1141 gaatacaccg gttacccgca tcggcgtgat tggagaggcg ctggcgcaag gccgttcagc
     1201 gattgaagcg gatgctgcct gtatctgaga tgtcatatcc ggtcagcatg tcggcatgcc
     1261 ggcgaaactc cggctcgcgc agcagcgcga agagtctctg cggccctggt tcgaaatacg
     1321 tccggcggcg catcacgagg tcgaagtgct cccaagcgag cggcacgaag cctagcgaat
     1381 tggctgcggc gtcggcgcgg gtcgcgaccc cgcagtcgat ctcgccggcg cggatcgcga
     1441 aggcgaggtc atggcccgtc gtgaagggat cccccttccg gttgagggcg tccagcggga
     1501 gtctggctgc cgcagccagg acttcgagga acagttgcgc gccggcgccg ctttgccgaa
     1561 agccaatgcg cgcctcgcga gatgctacat cggcgagcgt cacgagcttc agcggattgc
     1621 cggtcgcggt gagcaggccc tgttcgcgcc gcgcaaaggc gacgacgacc gcgtcagtca
     1681 gtttcgggca cgcctgcacg cccgcgatgt tgacgtccgc atcgctgccg accgcatgca
     1741 ggtgtatcgc ggccatggcg acctcgttgc ggtcgagctt ggcgagaccg gcgctcgaac
     1801 cttcgctgag cagcgccaat cccgaccccg aacgacgcgc tgcccattcg agcagcggat
     1861 cgtggctgcc gccgatgatc gggggcggtg caagaggcga gaaccctccc gggtgtgcga
     1921 ggttcgcttc cacccagcgg tcgagtgcgg cctgtgggaa cagccatttg cccgtcacct
     1981 tcgagcaagg caccgcgcca ttggcaacca gttcgtagag cttgcgctcc ttgatgccga
     2041 gataggcggc ggcttcctcg gtgttgagat aggctttcat gcgtttgatt gtttttaact
     2101 gcgtagattc cgaacttgag tatttctatg caaaacccat ataaaagcca acagaaaata
     2161 cggggttttc gggtcttgga tgtctccggc gccttcgctg ccgcatttgc gctcattgtt
     2221 ggcctcgatc cgcaattcct gcaggtcgtc ggactgtcgc tgcgcgtcac gctgacggcg
     2281 gtgctgctgg cggcgctgat tggcgttccg ctgggtgcct ggctggcgct ggcgcgtttt
     2341 ccgggacgct ctgcggtgat cgtgctggtc aacgggctga tggggctgcc gccggtggtc
     2401 gccggactga ttgtgttcct ggcgctttcg cgttccgggc cactcggcgg gctgggctgg
     2461 ctgttcacgc cgacggccat ggtgatcgcg cagacactgc tggtgctgcc gatcgtgctt
     2521 gcgctgaccc gtagcacgct cgaggaactc tggggcgaat accgtgacca cctgacctcg
     2581 cttggaatga cgggacttcg cgccattccc acgctgatct tcgatggccg cttcgcgctc
     2641 gcgacgggcc tgctcgccgg cttcgggcgg gcaagcgctg aggttggcgc ggtgctgatc
     2701 gtgggcggca acatcgccgg ctacacccgc acgatgacca catcgatcgc actggagacc
     2761 tcgaagggcg atctgcccct ggccctgggc ctgggcatcg tgctgatcgc gctcaccctg
     2821 gccatcaacg cggcagccta cggcgcgagc cgcgtcggcg cgcgcttcgc tgggtgatgt
     2881 ccatgcggga tacccccagc atcttgccgc tctcggtcca gaggctgtgt ttcgaaacgg
     2941 atggcaagcg ccttctggag gacgtgagct tcacgctccc gggcggtggg gtcaccacga
     3001 tcatcgggcc gaacggggcc ggaaagtcgc tgacgctcag gctctgccat ggtctgctca
     3061 cgccgacgtc cggcaccgtg cactgggggg ctgcaccggg tgcggcagtc gggcgcaaac
     3121 gccacgccat ggtgtttcag cggcccatca tgctccgtcg ctcggtccgg gccaatctgg
     3181 tgcacgcgct gtcggccgtt ggcgccgccg atgcggcact gcgcgtcaca gcggcgctgg
     3241 agcgcttcgg cctgaacgac atcgccgagc gcccggcacg cctgctgtcg ggcggcgagc
     3301 aacagcggct tgccatcgcc cgcgcctggt cgctgtcgcc cgaagtcctg tttctcgatg
     3361 agccgacgtc gcagcttgac ccgggcgcga ctcggcaaat cgaggggatc atcgccagcc
     3421 ttgcgtcgga aggaatgacc atcctcatga ccacccatga ccttggccag gcgcggcgcc
     3481 ttggcgtgcg catactgttg ctgaacaagg ggcgtctgat cgaggacgct gccacggccc
     3541 gatttttcgc cgctcctggc acccgggagg gccaggcttt catcgcagga gaactcctgt
     3601 ggtaacgacc cttcaaacca ggaagagagg aacccgacca tggtgaccaa acgcaacctg
     3661 ataaccgccg gcttcagcct cgcgttcacc ggccttgccc tcgcgcagac cgcacagcct
     3721 gccgcccccg cgcaatccgg cgcggccgcc gggactcggt tcatcacggt gtcctcgacg
     3781 acctcgacca ccgactccgg cctgttcaac caccttctgc ctgccttcaa ggccaagaca
     3841 ggcatcgagg tgcgcgtggt cagccagggc accggccagg cactggatac cggccggcgc
     3901 ggtgatgcgg acgtggtgtt cgtgcatgca cgcgcgcagg aagtgaaatt cgtcgaggac
     3961 ggcttcggcg tcgagcgcaa gccggtcatg tacaatgact tcgtcctgat cgggccaaag
     4021 caggatcccg ctggcatcaa gggctccagg gacattgccg ccgcgctgaa ggccattcag
     4081 gccaaggcca cgcccttcgt atcgcgcggc gacaagtccg gtacccattc cgccgaactc
     4141 aacctgtgga aggtggccga gatcgacatc gagaaaacca aggggccttg gtatcgcgag
     4201 atcggtcagg gcatgggcgc ggccctcaac acttccggcg cgatgggcgc ctatgtgctc
     4261 tccgaccgcg cgacctggct caatttcagg aatcgcggcg agcttgagat cgtggtcgag
     4321 ggggatcgtc ggctgtacaa ccagtacggc gtcattctcg tcaatccggc cagacacgcc
     4381 catgtcaaga aggccgacgg acaggccttc atcgactggc tgaccggccc cgaaggccag
     4441 aaggccatcg ccgactacaa gatcaacggc gagcagctgt tcttcgccaa tgcaacccag
     4501 ccaggggcgt aaggggcgaa gatcattcgc tgggctgttg ctcgccacgg ttgcgccggt
     4561 cgcagggggc gcaacagggc ggtaaagtga agaaaaatca gaatcttgaa tggtcggagt
     4621 ggcgggattc gaacccacga cccctagtcc cccagactag tgcgctaacc gggctgcgct
     4681 acactccgac tggcggcgct tataacaccg tagagcgcgg gcgcaatcca tgatcggcat
     4741 tccggcgagc gagcctttct tcaccacttg tgggtaggaa agtgcgggcg cacaccggca
     4801 attccgccgg cagcctggga atggcccatg cacgtcgtca tcattctgga ccaggcccat
     4861 gtcaacggtg gccaggccaa ggtcgcgctc gattcggctt tcggtcttaa ggcgcgcggg
     4921 cacacgcctg tcgtctttgc cgcctccggg ccggtgatgc cggaactgga agctgcggga
     4981 atcgagacgg tttgcctggg ccagcacgag tttgtgacgg atccgtcgaa agcggcggcg
     5041 gccttgcggg gactttggaa cttcgaagcg aggaaagcgc tggcaaccct tttggcgcgc
     5101 ctgccgcagg gcgatacggt ggtccacctg cacggctacg ccaaggcgct gtcgtcatcc
     5161 gtggtgccgg ctattcgggc cgctggcctg cccgtgctct acaccatgca tgagtactac
     5221 ctgttgtgcc cgaatggcgg cttctacaat taccgcgaga accaccactg cacgctaaag
     5281 cccctttcgc ccgcctgctg gaagactcat tgcgacacgc gcagttacgg attcaagctg
     5341 tggcgtggcg cgcggggtgt catctccagc cgcatcgccg acgtgttcgg aacgctgaca
     5401 gacatcgccc atttccacgg ctttcagaag cagatcatcg ccccgcactt gccccccgcc
     5461 acacgcttcc acgagatcgc caacccgatc gaagcggccg accttggtcc gaagtcaacg
     5521 cccgaacgcg gtgatttcgt cttcgtcggg cggctttcta cagagaaagg ctgtccgctt
     5581 ttcgcggagg cggcggcgcg agcgggggtc gtgccggcct tcgtcggcga tggacccgcg
     5641 gcggaagacc ttaagcgccg ctatccgcag gccagatttc atggctggca ggacatcgcc
     5701 ggagtgcgca gggcgctgcg cgaggcgcgc tgcctcgtct tcccttcgct ctggtacgag
     5761 ggccagccgc tgaccgtgct ggaatcgctt gcgatgggca cgccggtcat cgccggcgac
     5821 ggatcggcgg gacgagaaag cgtggtcgat ggcgtcaccg gactgtggtt caggcaggcg
     5881 gatgtgaatg atcttgctca gaaaatcacg atgatgcggg atgatgcgcg cgtcgccgcg
     5941 atgtcgcggg ctgcctatgg gcgctattgg gccgacccct tcacgctgga gcgccacgtg
     6001 tcccggctgg agcaggttta cggccgcttg ctgacgggtc agtaggcatt ttcgctcttg
     6061 atcagggcga acggcgtcag cagcaggatg tagaagtcaa aggcgagcga ccaattctcg
     6121 atgtagaaaa gatcgtgttc gacgcgcttc tggatctttt cgtgcgtgtc cgtttcgcct
     6181 cgccagccgt tcacctgggc ccagcccgtg atgcccggct tgaccttgtg gcgcgcgaaa
     6241 tagccctcga caacctcgtc atagagccgg tcttccgcct tggcgtgcac cgcgtgcggg
     6301 cgcggtccga ccagtgacag gtcgccgcgg aacaccacat tgaacagttg cggcaattcg
     6361 tcgagcgatg ccttgcgcag gaaacggccg acgcgggtca cccgcggatc atcccgcgtc
     6421 accagcctgg cggcggtcgc gtccgtctgc tcgacgtaca tcgagcggaa tttgaagacc
     6481 tcgatcatct ggttgttgaa gccatagcgc ttctggcgaa acagcaccgg tccgcgcgaa
     6541 tcgagcttca ccgccagggc gatcagcagc atcagcggcg tcagcgccag gagcagcagc
     6601 gcaccggcga cccggtcgaa tgccgccttc cacaccaggc cccagtcggc gaccggccgg
     6661 tcggccatgt cgaagaccgg catggagccg aggtaggaat agttgcgtgg cttgaagcgc
     6721 aactgctgcg tgtgcgcggc cagccggatg tccaccggca agacgcatac ccgcttcagc
     6781 atgtccagaa ttcgcgcttc cgcggtcacg ggcagggcga agatgatcgt gtcgagccga
     6841 gcgttccgtg catagccgat caactcgtca atgccgccaa gcttgggata accggcgacg
     6901 agatccggcg atcgcccatc cgtccggtcg tcgaacagtc cgacgatgcg cagatcgctg
     6961 tccttgtcgc gagtcaaggc ttccaggagg gcttcggccg ttgcgccgcc gccgacgatg
     7021 gccgcgcgcc ggtccagaag tccgcgtgcc gtcatggctt tcacggcaag gtggaccacg
     7081 aagcgtagcg cgagcagcgc cgccagtccg aacacgaacc aggcggcgaa cacgacccgc
     7141 gagaaggccc cctcgaattt gaggaagaag accagcgcga acagcacgag gaacaccagc
     7201 gcgaatcctg cgaccacctt ccaggcgctg cccacgaagt tccgaagggc tccgggttgg
     7261 tcgatgtcga gcaatcggaa agccaccaac gtcaacgcgg ctgcgccggg aagagcaacc
     7321 cagtacggtc tggcgctcac ttcgaccggc agatagaccc aggcgatagc cgcgccggtc
     7381 aggcagacga cgatgaattc caggatgcgc accgcgccgg aaatcacgat cggcgagatg
     7441 cgtccccgtc ccagcggcat gggggaaaac gcctcggcgg gccgaagccg caccgctccg
     7501 gttcccttcg cgccaatccc tgccgtttcg agggcgaggc caccctggcc ttccgcaagc
     7561 atcgcgcatc tcctgttgcg attggcttgc agaatagcag tttcgaccgt tgggttcgcc
     7621 ggtaagtcgc ggttaacctt gacgcgcggc cagcccggcc cggtaggccg cgacgatgtc
     7681 tctgcccatg cgctgtgcat cgaacagcgg gcggatgcga tccgccagcg cggccgcgcg
     7741 gatccgcaac gcgtcgtcgg acatggcatc ggcttccatg atcgcgccga ccagcctgtc
     7801 gagatcgtcg acgggcagca tccaagggta gtctgaaccg acaatctcgg gaatgccgcc
     7861 gcaactggtg gtgatcagcg ggatttgcgc cgcaacggat tccaggacga tgtagggcag
     7921 ggactcgaag cgggacggcg cgaccatgat gcggccgcgc gtcaggccaa cctgcgccgg
     7981 catcggcccg gtgaactgca gctgtccggc gattccttcc cgctcggcca gtgccttgag
     8041 ttcggcttcg tccggtccgc tcccgatgat cgtcgccgtc agggagcggc cctgcttctt
     8101 gatccggcaa atggcatgga tgagaagatc gatgcctttg acagggcgca actcgccgat
     8161 gaacacgatg tcggtggcgt cggcggccac ctgtcgtggc gtgaactcgt gcgggtgcaa
     8221 tccgttcagg gcgatgcgga cgatcttccg ggtctggccg acctccgcgc ggaacctgcc
     8281 cgcgatgaat tcgctttcga aggcgaagac gtcggtgccg cgttcgagca gggtctcgat
     8341 gaccatgtag acgcggtgcg ccatggtccc gggctggtag ttgaagctgc cgccgtgcgg
     8401 cgtgtagacc cgcacgggac cgcccattcc cagcgccgcg tcggctcggg cgtagagccc
     8461 gccctttgag ccgtggccat gcaggacatg cgcctgcgtt tccttcgcga tggcgcggat
     8521 gcgccggatg ttaccgatgt cggtccagtg cggattgcga tgcatcggca gacggtggat
     8581 gcccaaggca cattggtctt tgagctcggc aaacgtcgcc tcggcccgcg ggttgtccgc
     8641 gatcgtatcg gcgacgatgc ccacggagtg tccatcagcg atcaggtaac gcgccagatc
     8701 gatcacctgc cggaacaggc cgccgagtgg cgcgcggaac accagcagca cgcgcaaggc
     8761 gctcatgatc ctgtcaggtc cttgcagcag gggtcagaag aaccgttcgc gcacaagaat
     8821 cgtgtcgccc gggcggatgg gggtggtgat cggcacgttg acggtcagga ggtcgccgtt
     8881 cacgacgcgc gtcacctcag catagtgacg tgcggcgcgc gcggaaaacc ctccggcgat
     8941 cgccacggcg gtctggatgg tcatgccgtt gacgtaaggg tattgtcccg atgtggtcac
     9001 ttctccgaga atgaagaagg gccgataggt atcgacctcg acgctgacct tgggttcgcg
     9061 caggaagccg gcgcgcagac ggccctcgat cgagcgttcc agcccggcgg ttgtcaagcc
     9121 gcgcgcctcg accgtgccga tcagcggcag cgagatgcgc ccggcgccat ctacggcata
     9181 gacgttcgac agtgcatcct gtccgaacac gatcacccgc agcttgtcgc cggaattgag
     9241 cgtgtagggc tggctcaggc tcgcctgcag cacatccggc ggcatgatgg gtggcgagca
     9301 ggccgcaacg gtcagcgcgc aggtcaggga aaggagaata cggagcaagc gcatcgcgac
     9361 agtactggtg attgcgaaca gccccgacaa tgcgcgggtt atggttaaca ctttaccaat
     9421 tttggcgaat tgggagcgcc ttaacgcacc actaaccatg ttcgttcgta atcccttcga
     9481 atttgcgttc ggagaatcgg ttcatgcgtg gtcccataca gccggatttc gggcaggcgc
     9541 ccgccgccga cgccagtctc ggaggcgtgt tcgcggcgct caaccggcgc aagtggtgga
     9601 tcctgatggc gacgacgatg gcgttcgtcg ccacggcggc gttcgtgaac atcgtcacgc
     9661 cgagctacac cggcgaagcc cggcttctgc tggagaaccg cgacaactac tttacccggc
     9721 ccgatcgcga ttcccgtggc gccgagccat cgattgacgc ggaagcggtg cagagccagg
     9781 tgcaactgat cctgtcgcgc gacatcgcgc gcgccgtgat caagaagctg gatctttcat
     9841 cgaaggagga gttcgattcc gcccgccgtg aagcgagcag cctgaagcgt cttctcgcga
     9901 tcgccggact ggccaaggac ccgctggcct cgtcgccgga ggagcgtgtc ctggaagcct
     9961 acatggacaa gctgcttgtg gtcactgtcg gcaagtcccg tgtcatctcc gtcagttttt
    10021 cctcgaagga ccctgatctg gctgcccgcg ccgccaacac ggtggccgac gagtacatcc
    10081 ggttccaggg cgacgccaag aaatcgacca cccagggtgc cagcgcctgg ctttcgcaga
    10141 ccatcgagac gctcagggcc aaggtcgccg aggctgaagc caaggtcgag aactaccgcg
    10201 cagccaacgg gttgctcatc ggctcgtcga acgcgaccat caccacccag caactcggcg
    10261 agatgaacac gcagatctcg aacgcgcgtt cggcgcagtc ggatctgcaa gccaaggccc
    10321 ggttgattcg cgaggccctg aagaacgggc gcatcttcga aacctccgat gtggtcaaca
    10381 acgaactcat ccgccgcctg ctggaacagc gcgtcgcgat gaagacgcag atcgcactgg
    10441 aagagcgcac gctgttgccg ggccatccgc gcatgaagga actgaccgct cagctcaatg
    10501 acctggaggg tcagatccgc accgccgcgg agcgcacggc ccggacactg gagaacgacg
    10561 cccgcgttgc cggcgcgcgc atgcagagcc tgcaagccga gttcgaggga cagaagcgca
    10621 ccgccgccga cgccaacggc agcgaggtcc agcttcgcgc cctggaacgc gaggcacggg
    10681 cgctgcgcga caattacgaa tcctatctgg cgaaataccg cgattccctt tcgcgcgatt
    10741 ccgatgccgc ctcgccggcc gatgcccgca tcttctcccg cgctatcgtt ccgcagaagc
    10801 cggccttccc gaaaaagctg ccaatgatcc tgctggcgac gctggccacg ctcatcctga
    10861 gctgcgccat ggtcgtcacg cgtcaggtct tcatgaccga tccggctggt gagatcatgc
    10921 cgccggcgat ggtcgatccc cacgggcgct tcgttgcgcc ggaagcgccg cgccaccatc
    10981 ccgggcagtt ccaggcgtcg cctccggtcg cggacccgtc attcaatccg caaccggcct
    11041 ggcatccggc gccgccggct gcacctccgg cagcggccag ggttcgggag cctcaggtcc
    11101 aggcggcagc cccccctccg gtggccgagc ctccggtgct ggcgcccgaa caggcggccg
    11161 aaagactcca ggcgacc
//
LOCUS       DAOMXN010000060        11102 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_3165_length_11102_cov_12.025980, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000060 DAOMXN010000000
VERSION     DAOMXN010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 11102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11102
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3165_length_11102_cov_12.025980"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="PK812_06300"
     CDS             <1..461
                     /locus_tag="PK812_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HRJ69202.1"
                     /translation="PADIAAVTRGMPDFSDLPEPLHQRAAELGEGSVRRALSVLEPKR
                     LAFNDRVEATLAGLPHLDGNEIDAIAEQTAGRTGEEAFVRFCELCESWLAREMAERPR
                     DVAALAPLSELWNRLGEGRRDVEAYNLDRRPFVIAMIADLAAAARRPVRA"
     gene            complement(451..1545)
                     /locus_tag="PK812_06305"
     CDS             complement(451..1545)
                     /locus_tag="PK812_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007199173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRJ69203.1"
                     /translation="MTQVIRYGIIGAGMMAREHMRNLALIPGSVVAAIADPDAESRRL
                     SGESAGPQARLFADHHSLLAAGDCDALIIASPNDTHAAILGDIFAQSRALPVLVEKPV
                     CTRLEECDALERAAAAHRAPVWVAMEYRYMPPVAELRQRVSAGEVGALHMLTIREHRF
                     PFLEKVGDWNRFSERTGGTLVEKCCHFFDLMRLIMADEPVRVYASGAMDVNHRGERYG
                     GRMPDIIDNAYCIVDFRSGRRAMLELCMFAEGSYWQEIITAVGDRAKVECFVPGPARF
                     WPGGGERESEIEISPRDPKGPRRSTVHVDPHVLKAGDHHGSTYYQHLGFRRAVLDGGA
                     VDVTMADGLMAVKIGLAAERSIKEGRAIML"
     gene            complement(1598..2428)
                     /locus_tag="PK812_06310"
     CDS             complement(1598..2428)
                     /locus_tag="PK812_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRJ69204.1"
                     /translation="MTSSQKKRNITWLAALIVAANGFIPAIWVLFTSLKTEGELNRIP
                     ITIFPNQPTLENFTRVFVDQPILVFIANSVAVAIISTVICVLVSALAAYALVRLRLPA
                     PGLILSVILAIAMFPLISILVPLFAVMRDAGLLNTWWALIFPYAVLSMPVCTMVLIAF
                     FQDIPADLENAAMIDGCTRVGALFRVVVPLSAPGVFTAAILAFVNAWDEFLLALTLSP
                     RVMSRTLPVGITLYQGEFAFPWPIMSAALVVAITPVCVLIAIYQDKIVSGLTQGGMKG
                     "
     gene            complement(2433..3359)
                     /locus_tag="PK812_06315"
     CDS             complement(2433..3359)
                     /locus_tag="PK812_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019997638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRJ69205.1"
                     /translation="MTSATPVLKHRLTPLERSERRLGLVMLAPAFILLAATVIYPVAT
                     LIWTSFRFQHLAQPWMGTPFVGLEQYWKGLTDPHFWSSTLNTALYILVTVPGAVIVGL
                     GLALLANQPFRIKWPVRLGLLLPWALPLVFAGLIFRWFFEFKQGIVNNALIWFGLKPL
                     NWLTDPTLAFIAICIAIIWKSSSFVALVLLAGLQTIPKSMYEAAEIDGANKWQQFVEI
                     TLPMLRPAIFVALIFRTITAIQTFDIPYAMTNGGPGNSTETLAMYIHKTTIDFLDFGY
                     GSALAVLMFIVSVIATSGYLRYTRRDPSKAGR"
     gene            complement(3444..4709)
                     /locus_tag="PK812_06320"
     CDS             complement(3444..4709)
                     /locus_tag="PK812_06320"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRJ69206.1"
                     /translation="MRKGIVASVVGASLMALGLATTPAEAQNLRIFTGGQQRPDVMRK
                     ILDVFQERNPGMKVDIEVGGATSEQQQQYLNTVLASRDASLDVVLIDVIRPAQWAAAN
                     WAEPLDGFLGAEKDAVMAKYLPAYRAANIVNGKVIALPYFADAQFLYYRKDLLEKHKQ
                     QPPKTWAELQASAGAVMKGEGNANLQGFLTAGAPIEGTVCTYLVPVWGSGGDLTDSGG
                     KLALGADAAKAPFKLYADMKTAGVLPANIAEIVTDRARQMFQAGGAVYNMNWAFNWNR
                     FQNDADSQVKDKVGVIPLPGFTADRQSTCIGGWQLAITAFSKNKEAAYKLLRFLSSPE
                     VSKMQAIAASHLPVFAEVYADADVLKANPWFKDALPVVQSARSRPVSARYTEVSEIVR
                     TNMNAYLAGTKTAEQALGDMTTRLGAIYR"
     gene            complement(4750..5862)
                     /gene="ugpC"
                     /locus_tag="PK812_06325"
     CDS             complement(4750..5862)
                     /gene="ugpC"
                     /locus_tag="PK812_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HRJ69207.1"
                     /translation="MASVDIVGVRKSYGPVPVIHGVDIAIGDGEFVVLVGPSGCGKST
                     LLRMIAGLESISGGDIRIGGDRVNDLPPRSRDIAMVFQDYALYPHKTVRENIGFGLRM
                     RGAAPETVATKVAEAAQILQIGHLLDRRPGQLSGGQRQRVAMGRAIVREPRAFLFDEP
                     LSNLDAQLRSEMRVEIKKLHQRLQNTMIYVTHDQVEAMTLADRIAVMRSGHLEQLGTP
                     DDIYNRPRTRFVAGFTGSPPMNFADCAIAADGASLTLGDVTLSLPGELRSAARSLSGR
                     KVSFGIRPEHIQAGSRPGDGFAQVRGHVVLTEPLGSETLARVKIGNAELTGRFEPDLG
                     LKVGQTVDLSLAMARAHLFDEETGLSALWGAAGGKS"
     gene            complement(6001..6237)
                     /locus_tag="PK812_06330"
     CDS             complement(6001..6237)
                     /locus_tag="PK812_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69208.1"
                     /translation="MIHSRKQLGIDMLALSALLLRIFTKTPEWAAPRSNLAQALIETG
                     DRPRACAAAANGRGREMEAVASDMANFKRRAGCP"
     gene            complement(6244..7938)
                     /locus_tag="PK812_06335"
     CDS             complement(6244..7938)
                     /locus_tag="PK812_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRJ69209.1"
                     /translation="MTNPGFPPRPYPWEKSYPPGVRWDFPLETGPVGKVLDNGIAGYP
                     DRPALSYGARTWTYAAFGDLVARAATGLMTLGVGRGTPVALYLPNTPWHPVLFFAVMR
                     LGGIVVHLSPLDAERELAHKLKDSGARIVATTDLGPMAGAARLLQDRFAATGIIDTII
                     VGEDAVWREDRLQRTAEAGPIRSGAALLDHAPLPASAFAPVDPAEVAVLQFTGGTTGM
                     PKAAALSHANLYAACEMYRAWAEPQNLLRYGAEKTLMVLPLFHIYGLTTVLMFGLRNG
                     AELVLHPRFDVAAVVDDIERRGITQFPGVPTMWIAIANFPGIETRNLSSLRTVSSGGA
                     SCPVEIEARIGKLTGKRLGGGWGMTETAPAGSRIPLHKPAPAGTIGLPVPGLHMDIVA
                     LDDPHRRLGVGEIGEIRVKGPNVIKGYHNRPEENAAAFADGYLLTGDMGYTDAEGFFF
                     IVDRKKDMITSGGFNVYPRVVEEAVYEHPAVAECIALGVPDAYRGAAIKVFVALKPGA
                     PALTLDALRAFLADKIGRHEMPAHLEIREALPRTPVGKLSKKALQDEERASATTAR"
     gene            complement(7959..8804)
                     /locus_tag="PK812_06340"
     CDS             complement(7959..8804)
                     /locus_tag="PK812_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRJ69210.1"
                     /translation="MRFAGTDSYVATDDLKVAVNAAIVLQRPLLVKGEPGTGKTVLAE
                     EIARATGSHLITWHIKSTTKAAQGLYEYDAVSRLRDSQLGDPRVSDIRNYIRKGKLWE
                     AFSSDQRPVLLIDEIDKADIEFPNDLLLELDRMEFHVYETGETVKAAQRPIVVITSNN
                     EKELPDAFLRRCFFHYIKFPDGETMQKIVDVHYPGIKRQLVAAALNVFFQVRDTPGLK
                     KKPSTSELLDWLKLLVSEDISPEALKERDQRKLLPILHGALLKNEQDVSLFEKLAFLS
                     RREGR"
     gene            complement(8874..9092)
                     /locus_tag="PK812_06345"
     CDS             complement(8874..9092)
                     /locus_tag="PK812_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69211.1"
                     /translation="MKMMTQYAADTASTARVLTLRGHQSGLWELSDNLGRLGGVFVDM
                     KSAIRFAKMECGEAIVLIDARHGAATTH"
     gene            9214..10392
                     /locus_tag="PK812_06350"
     CDS             9214..10392
                     /locus_tag="PK812_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRJ69212.1"
                     /translation="MLLHFFTSLREAKVPVTLREYLTLIEALKADLANATVEDFYYLS
                     RALLVKDERNLDKFDRVFGTVFKGLEGAAADGIAPVDIPEEWLRKLAEKYLSDEEKAK
                     IEALGWDKLWETLKQRLEEQKGRHQGGNKWIGTGGTSPFGAYGYNPEGIRIGQAGNRN
                     FRAVKVWDKREFKDLDGKAELGTRNIKIALRRLRRFARTGALDELDLDGTIRGTAHKG
                     YLDVHMRAERRNAIKVLLLLDIGGSMDWHVEQADELFSAAKSEFKHMEHFYFHNCLYE
                     RVWKENRRRDQSSLSTAELLRTYGGEYRVVFVGDASMSPYEIMQPGGSVEHFNEEAGA
                     VWLQRMLDHFPKSVWLNPIPEQHWGYTHSIRMIQQQFSGRMFPLTLEGLDGAMRALSR
                     "
     gene            10401..10889
                     /locus_tag="PK812_06355"
     CDS             10401..10889
                     /locus_tag="PK812_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016650637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HRJ69213.1"
                     /translation="MEPILVLIDADACPVKEETYKVAARHGVRTIVVSNTWMNVPRGD
                     PLIEQVVVPAGLDVADDWIAERARRGVVVITADIPLAARSLRAGADAIAPNGRPFTEQ
                     SIGNVLATRNLMDDLRSAGAITGGPKPFAPKDRSAFLSALDLAIVRLKRAGFGGAAQV
                     AP"
     gene            complement(10927..>11102)
                     /locus_tag="PK812_06360"
     CDS             complement(10927..>11102)
                     /locus_tag="PK812_06360"
                     /inference="COORDINATES: protein motif:HMM:NF024761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HRJ69214.1"
                     /translation="PYVNWGKVNANIPKAMAADDITLEQAIDLLRAREASGGGTKAKA
                     PAKKPARKAAKKS"
BASE COUNT         1977 a         3537 c         3694 g         1894 t
ORIGIN      
        1 aaccggccga catcgccgcg gtcacgcggg gcatgccgga tttttcggac ctgcccgagc
       61 cccttcacca gcgcgccgca gagctggggg aagggtccgt gcgccgcgcg ttgtccgtgc
      121 tcgaacccaa gcgcctggcc ttcaatgacc gggtggaggc gaccctcgcc ggcctgccgc
      181 acctcgacgg caacgaaatc gatgcgatcg ccgagcagac cgccgggcga accggcgagg
      241 aagcgttcgt ccggttctgc gagttgtgcg aaagctggct ggcgcgcgaa atggcggaac
      301 ggccccgcga tgtcgcggcg ctcgcaccgt tgtccgaact ttggaatcgc ctgggcgagg
      361 gacggcgcga cgtcgaggcc tacaatctcg accgtcgccc tttcgtcatc gcgatgatcg
      421 ccgacctggc cgccgccgcg cgtcgaccgg tcagagcatg atcgccctgc cttccttgat
      481 cgagcgttcc gccgccaggc ctatcttcac cgccatcagc ccatccgcca tggtcacatc
      541 gacggcgccc ccgtccagaa cggcgcgccg gaagccaagg tgctggtaat aggtcgaccc
      601 atgatggtcg ccggccttca gcacatgcgg atcgacatgg accgtgctcc gcctcgggcc
      661 tttcggatcg cgcggggaaa tctcgatctc gctctcccgc tcgccgccgc ccggccagaa
      721 gcgcgccggc cccggcacga agcactcgac cttcgccctg tcgccgaccg ccgtgatgat
      781 ctcctgccag tacgagcctt ccgcgaacat gcaaagctcc agcatggcgc ggcgcccgga
      841 gcggaagtcg acgatacagt aagcgttgtc gatgatgtcc ggcatgcgac cgccgtatcg
      901 ctcgccccgg tggtttacgt ccatggcgcc ggatgcataa acgcggaccg gctcgtcagc
      961 catgatcagg cgcatgaggt cgaagaagtg gcagcatttc tcgacgagcg ttcccccggt
     1021 gcgttcggaa aaccggttcc agtcgccgac cttttcgagg aacggaaagc ggtgttcgcg
     1081 gatcgtcagc atgtgcaagg cgccgacttc gccggctgag acgcgctggc gcaactcggc
     1141 gaccggcggc atgtagcggt attccatcgc cacccagacc ggcgcgcgat gcgctgcggc
     1201 ggcgcgctcc agtgcgtcgc actcctcaag gcgcgtgcag accggcttct ccaccaggac
     1261 cggcagcgca cgcgactgcg cgaagatgtc gcccaggatg gcggcatggg tatcgttggg
     1321 gctggcgatg atgagtgcat cgcagtcgcc tgccgcaaga aggctgtggt gatccgcgaa
     1381 gagcctggcc tgcggcccgg cggattcgcc ggacagacgg cgggactcgg cgtccggatc
     1441 ggcgatggcg gccacgacgg agcccgggat cagcgcgagg ttgcgcatgt gctcacgggc
     1501 catcatgccg gcgccgatga tgccgtatcg aataacttgg gtcatggtct gatgttcctg
     1561 aaggcttcgg gcggttcgca gggaccgaag ccctgcttca gcccttcatg ccgccctgcg
     1621 tcaagccgga gacgatcttg tcctgataga tggcgatcag cacgcagacc ggggtgatcg
     1681 ccaccaccag tgccgccgac atgatcggcc agggaaaggc gaattcgccc tggtagagcg
     1741 tgatacccac cggcagcgtg cggctcatca cgcgcggcga gagcgtcagc gccagcagga
     1801 attcgtccca ggcgttgacg aaggcgagga tcgccgcggt gaacacaccc ggagccgaca
     1861 gtggcaccac cacacggaac agcgccccca cgcgggtgca gccgtcgatc atcgccgcgt
     1921 tctcgaggtc ggccggtatg tcctggaaga aggcgatcag caccatggtg cacaccggca
     1981 tcgacagcac ggcataggga aagatgagcg cccaccaggt gttcagcagt ccggcgtcgc
     2041 gcatcaccgc gaacagcggc acgaggattg aaatcagcgg gaacatggcg atggcgagga
     2101 tcaccgaaag gatcagcccg ggcgccggta gtcgcagacg tacgagcgcg taggccgcca
     2161 gcgccgacac gagcacgcag atcaccgtgg aaatgatcgc taccgcgacc gagttcgcga
     2221 tgaagacaag gatgggctgg tcaacgaaga cacgggtgaa gttctccagc gtcggctggt
     2281 tcgggaagat ggtgatgggg atgcggttga gctctccctc ggtcttcagc gaggtgaaca
     2341 gcacccagat cgccgggatg aaaccgttcg ccgccacgat cagcgcggcc agccaggtga
     2401 tgttgcgctt cttctgcgaa ctggtcatgg cgtcacctgc cagccttcga gggatcgcgc
     2461 cgggtgtagc gcaggtagcc cgaggtcgcg atcaccgaca cgatgaacat cagcacggcg
     2521 agcgccgacc catagccgaa atcgaggaaa tcgatcgtcg tcttgtggat gtacatcgca
     2581 agcgtctcgg tggagttgcc cggcccgcca ttggtcatgg cgtagggaat gtcgaaggtc
     2641 tggatggcgg tgatggtccg gaaaatcaga gccacgaaga tcgcggggcg cagcatgggc
     2701 agcgtgatct cgacgaactg ctgccacttg ttggcgccgt cgatctcggc tgcctcatac
     2761 atggacttcg gaatcgtctg gaggccggcg agcaggacca gcgcaacgaa actcgacgat
     2821 ttccagatga tcgcaatgca gatcgcgatg aaggcaaggg tcgggtcggt cagccagttc
     2881 agcggcttca atccgaacca gatcagcgcg ttgttgacga tgccctgctt gaattcaaag
     2941 aaccagcgga agatcaggcc ggcaaacacc agaggcagcg cccagggcag caacaggccg
     3001 aggcgcacgg gccacttgat gcggaagggc tggttggcga gcaacgccag gccaagacca
     3061 acgatcaccg cgccgggcac ggtgaccaga atgtacagcg cggtgttgag cgtcgatgac
     3121 cagaaatgcg ggtccgtgag gcccttccag tactgctcca ggccgacgaa gggcgtgccc
     3181 atccagggct gggcgagatg ctggaaacgg aacgaggtcc agatcagcgt cgcgaccgga
     3241 tagatcaccg tggcggccag caggataaag gccggcgcca gcatcaccag gcccagccgc
     3301 cgctcggatc gctccagcgg ggtgaggcgg tgtttgagga caggcgtcgc gctggtcatg
     3361 accggccccg ggtcggatgt tgggtcgaga agtgcctgcg accctccgcc gccaagcgcg
     3421 gcggagggct tcttgttctg gcatcagcga tagatggcgc cgaggcgggt cgtcatgtcg
     3481 ccaagcgcct gctcggcggt cttggtcccg gcgaggtagg cgttcatgtt ggtgcgaacg
     3541 atttccgata cctcggtgta gcgcgccgag accgggcggg agcgggcgga ttggacgacc
     3601 ggcagcgcat ccttgaacca ggggttggcc ttcaggacgt cggcatcggc gtagacctcg
     3661 gcaaagaccg gcaggtgcga ggcggcgatc gcctgcatct tcgagacttc cggcgaggac
     3721 aggaagcgca gcaacttata ggccgcttcc ttgttcttgg agaacgcggt gatggcgagc
     3781 tgccagccgc cgatgcaggt cgactgcctg tccgccgtga agcccggcag cggaatcaca
     3841 ccgaccttgt ccttcacctg cgagtcggca tcgttctgga aacggttcca gttgaacgcc
     3901 cagttcatgt tgtacaccgc accgccggcc tggaacatct ggcgcgcgcg atcagtcaca
     3961 atctcggcga tgttggccgg gagcacacca gcggtcttca tgtctgcgta gagcttgaac
     4021 ggtgccttgg cggcatcggc gcccagagcc agcttgccgc cggaatcggt caggtcaccg
     4081 cccgaacccc agaccggcac gagataggtg cacaccgtgc cttcgatggg cgcgccggcc
     4141 gtgaggaagc cctgcagatt ggcgttgccc tcgcccttca tcacggcgcc ggccgatgcc
     4201 tgcaactcgg cccaggtctt cggcggctgt tgcttgtgct tctccagcag gtccttgcgg
     4261 tagtaaagga attgcgcatc ggcgaagtag ggcagggcga taaccttgcc gttgacgatg
     4321 ttcgcggcgc ggtatgccgg caggtatttc gccatcaccg cgtccttctc ggcgccgagg
     4381 aagccgtcca gcggttccgc ccagttggcc gccgcccatt gcgccgggcg gatgacatcg
     4441 atcagcacca cgtcgagcga cgcgtcacgc gaggccagaa cggtgttgag atactgctgc
     4501 tgctgctcgg atgtggcgcc gccgacctcg atgtcgacct tcatgccggg attgcgctcc
     4561 tggaacacgt ccaggatctt gcgcatcaca tcggggcgct gctggccgcc ggtgaaaata
     4621 cgcaggttct gcgcctcggc cggcgttgtc gccaggccca gcgccataag ggatgccccg
     4681 acgacgctgg caacgatgcc ttttctcatg tcttcctcct tcagcggcct tcctgccgcg
     4741 gttcttcgct catgacttgc cgcccgccgc gccccagagc gcggacaggc cggtttcctc
     4801 atcgaacaga tgcgcccggg ccatcgcgag cgacagatcg acggtctgac cgaccttcag
     4861 gccgaggtcc ggctcgaaac ggccggtcag ttccgcgttg ccgatcttga cccgagccag
     4921 cgtctcggaa ccgagcggct cggtcagcac gacatgaccc cgtacctggg caaagccatc
     4981 acccggtcgc gaaccggcct ggatgtgttc cggccgtatt ccgaagctga ccttgcgccc
     5041 ggacaacgag cgggcagcgc tgcgcagttc tccaggcagc gacagcgtca catcgccaag
     5101 cgtcagcgag gcaccgtcgg cagcgatcgc gcaatcggcg aaattcatgg gcggggaacc
     5161 ggtgaacccg gcgacaaacc gggtgcgcgg gcgattatag atgtcgtccg gcgtgcctag
     5221 ctgctccaga tgaccgctgc gcatcaccgc gatccggtca gcgagcgtca tcgcctccac
     5281 ctgatcgtgc gtcacataga tcatggtgtt ctgaagacgc tggtgcagtt tcttgatctc
     5341 cacccgcatt tcgctgcgca gctgggcgtc caggttggag agcggctcgt cgaacagaaa
     5401 ggcgcgcggc tcgcgcacga tggcgcggcc cattgcgacg cgctggcgct gaccgcccga
     5461 gagctgaccg ggtcggcggt cgagcaggtg cccgatctgg aggatctgcg cggcttccgc
     5521 cacctttgtc gcgaccgtct ccggcgccgc gccacgcatg cgcaggccga agccgatgtt
     5581 ctcgcgcacg gtcttgtggg gatagagggc gtagtcctgg aacaccatcg cgatgtcgcg
     5641 cgaacgcggc ggcaagtcat tcacgcgatc cccgccaatg cggatgtcgc cgccggaaat
     5701 cgactccagc ccggcaatca tccgcaagag cgtcgacttt ccgcagccgg aggggccaac
     5761 cagaacgacg aattcgccgt cgccgatggc gatgtcgacc ccatggatga cgggcacggg
     5821 accgtaactc ttgcggacac cgacgatgtc gaccgaagcc aagcgcgcct cccgggcagc
     5881 aatgctctct ctgctatatt ttatatctta tataagactg gatcgcgcga tggtcaatcc
     5941 gtgtgaatcc ggttgtcgga ccatccggca tgggcgtggc gggggtggcg ggtctgccgg
     6001 ctaagggcag ccggcccggc gcttgaaatt ggccatgtcg gaagccaccg cttccatctc
     6061 cctgccacga ccgttcgccg cggcggcgca ggcccgcgga cggtccccgg tctcaatgag
     6121 agcctgcgcg agattgctgc ggggcgcggc ccattccggt gtcttcgtga agatgcgcag
     6181 caacagtgcg gacaaggcca gcatgtcgat gcccaactgt ttccgcgagt ggatcacgcg
     6241 ctctcagcga gcggtagtcg cgctcgcgcg ttcctcatcc tgcaaggcct ttttcgacag
     6301 tttgccgacg ggcgtgcgcg gaagggcctc gcggatttcg agatgcgcgg gcatttcatg
     6361 ccggccaatc ttgtcggcga ggaaggcccg caatgcatcc agcgtgagcg caggggcacc
     6421 cggctttagc gccacgaaga cctttatggc agcgccgcga tacgcgtcgg gaacgcccag
     6481 agcgatgcat tcggcgaccg ccgggtgctc gtagaccgct tcctccacca cgcgggggta
     6541 aacgttgaac ccgccggagg tgatcatgtc cttcttgcgg tcgacgatga aaaagaatcc
     6601 ctcagcgtcc gtgtagccca tatcgccggt cagcaggtat ccgtcggcga aggcggccgc
     6661 attctcttcc gggcggttgt gatagccctt gatgacattc ggccccttga ctcggatttc
     6721 gccgatttcg ccgacgccga gccgccgatg cggatcgtcc agcgcgacga tgtccatgtg
     6781 caggccggga accggcaagc cgatggttcc tgccggggct ggcttgtgga gtggaatgcg
     6841 ggagccggcc ggcgcggttt cggtcatgcc ccagccgccg ccgagccgct tgcccgtgag
     6901 cttgccgatg cgcgcctcga tctccacagg gcaggacgcg ccgccggaac tgacggtccg
     6961 cagcgaggac aggttccgcg tctcgatacc ggggaaattg gcgatggcga tccacatcgt
     7021 cggaacaccg gggaactggg tgatgccgcg ccgctcgatg tcatccacca cggccgcgac
     7081 gtcgaagcgc ggatgcagca ccagttccgc gccgttgcgc agtccgaaca tcagcaccgt
     7141 ggtgaggccg tagatgtgga acagcggcag caccatcagc gtcttttccg cgccgtagcg
     7201 cagcagattc tgtggttcgg cccaggcgcg atacatctcg caggccgcgt agagattggc
     7261 gtggctgagc gccgcggctt tcggcatccc ggtagtgccg ccggtgaact ggaggacggc
     7321 gacctcggcc ggatcgaccg gcgcgaaggc gcttgccggc agcggagcat ggtccagaag
     7381 cgctgcgccg gagcggattg gaccggcttc cgcagtgcgt tggagcctat cctcgcgcca
     7441 gaccgcatct tcgccgacga tgatcgtatc aatgatccct gtcgcggcga accgatcctg
     7501 cagaagccgc gccgcgccgg ccatcggccc gagatcggtg gtcgcgacga tgcgcgcgcc
     7561 ggaatccttc agcttgtggg caagctcgcg ttcggcgtcg agcggggaga gatgcacgac
     7621 gatgccgccg agccgcatca ccgcgaagaa cagcacgggg tgccagggcg tgttcggcag
     7681 atagagcgcc accggcgtgc cgcgaccaac cccgagagtc atgagcccgg tcgccgcccg
     7741 cgccacgagg tcgccgaagg ccgcataggt ccaggtccgc gccccgtagc tcagcgcagg
     7801 ccggtccgga tagcccgcga tgccgttgtc cagcaccttg ccgaccggac cggtctcaag
     7861 cggaaaatcc cagcgcacgc ccggcggata ggacttttcc caagggtagg ggcggggcgg
     7921 gaagccggga ttggtcaccg cccgttgccc cggttcgctc agcgcccttc gcggcgcgac
     7981 aggaaggcga gcttttcaaa caggctgacg tcctgctcgt tcttcaacag cgcgccatgc
     8041 aggatcggca gcagcttgcg ctggtcgcgt tccttcagcg cttcgggcga aatatcctcg
     8101 gagacgagca gcttcagcca gtccagcagc tcggaggtgg agggcttctt cttcaggccc
     8161 ggcgtgtcgc gcacctggaa gaacacgttg agcgccgccg cgacaagctg ccgcttgatg
     8221 ccgggataat gcacgtcgac gatcttctgc atcgtctcgc catccgggaa cttgatgtag
     8281 tggaagaagc agcggcgcag gaatgcgtcc ggcagttcct tctcgttgtt cgaggtgatg
     8341 acgactatgg ggcgctgcgc ggccttcacc gtctcgccgg tctcgtagac atggaattcc
     8401 atccggtcga gttcgagcag caggtcgttc gggaactcga tatccgcctt gtcgatctcg
     8461 tcgatcaaca gcaccgggcg ctggtccgaa gagaatgcct cccagagctt gcccttgcgg
     8521 atgtagttgc ggatgtccga gacgcgcgga tcgccgagct ggctgtcgcg caggcgcgag
     8581 accgcgtcgt actcatagag gccctgcgcg gccttggtgg tggacttgat gtgccaggta
     8641 atcaggtggg agccggtcgc cctggcgatc tcctcggcca gcacggtctt gccggttccg
     8701 ggctcgcctt tgaccagcag ggggcgctgg agcacgatag cggcgttgac cgccaccttg
     8761 aggtcgtcgg tggcgacata ggagtcagtg ccggcaaaac gcattgttca gttccaaacc
     8821 atcacgatac tgttgtttgg aacgcccgat gcgagcggtc aataggctgg cggtcaatgc
     8881 gttgtggcag cgccatgcct ggcgtcgatc aggacgatgg cctcgccgca ctccatcttc
     8941 gcaaaacgga tcgccgactt catgtcgacg aacacgccgc cgagacggcc gaggttgtcg
     9001 ctcagctccc agaggccgga ctggtgcccg cgcagggtca gcacgcgtgc ggtggacgcg
     9061 gtgtcggccg catactgggt catcatcttc atggcgaacc tcctcggttt gccccacgct
     9121 ggactctata ggcgaagcgc cggggcggtg cagtgcggcg gcgcacaggg cagggtggct
     9181 tgaccttccg gccccgccgg ggaagaaagc gtcatgctgc tccatttctt cacttcgctg
     9241 cgcgaggcca aggttcccgt cacgctgcgc gagtacctga cgctgatcga ggcgctcaag
     9301 gccgatctcg ccaatgccac cgtcgaggat ttctactatc tctctcgcgc gctgctggtg
     9361 aaggacgagc gcaacctcga caagttcgac cgggtgttcg gtaccgtctt caaggggttg
     9421 gaaggcgcgg ctgcggacgg catcgctccg gtcgacattc ccgaggagtg gctgcgcaag
     9481 ctcgccgaga aatatctgtc cgatgaggaa aaggccaaga tcgaggcact gggctgggac
     9541 aagctctggg agacgctgaa gcagcgactg gaggagcaga agggacgcca tcagggcggc
     9601 aacaagtgga ttggcacggg cggtacctct cccttcggcg cctatggcta caatccggaa
     9661 ggcatccgca taggtcaggc cgggaaccgc aacttccgcg cggtgaaggt ctgggacaag
     9721 cgcgagttca aggatctcga cggcaaggca gaactcggaa cccgcaacat caagatcgcg
     9781 ctcaggcgcc tgcgccggtt cgcgcgcacc ggggcgctgg acgagcttga cctcgacggc
     9841 acgatcaggg gcacggccca caagggctac ctcgacgtgc acatgcgcgc ggagcgccgc
     9901 aacgccatca aggtgctgct gctgctcgac atcggcgggt cgatggactg gcacgtggag
     9961 caggcggacg aactgttctc ggccgccaag tccgagttca agcacatgga acacttctat
    10021 ttccacaact gcctctacga gcgggtgtgg aaggagaacc gcaggcgcga ccagtcctcg
    10081 ctgtcgacgg cggaactcct gcgcacctat ggcggcgaat atcgcgtcgt cttcgtcggc
    10141 gatgcctcga tgagccctta cgagatcatg cagcccggcg gctcggtgga gcacttcaac
    10201 gaggaggccg gagcggtctg gctccagcgc atgctggatc atttccccaa gtcggtgtgg
    10261 ctgaatccga tcccggagca gcactggggc tacacccact ccatccgcat gatccagcag
    10321 cagttttcgg gccgcatgtt cccgctgacg ctggaaggcc tcgatggtgc gatgcgggcg
    10381 ctgagccgat aggacgctgc gtggagccga tcctcgtcct catcgacgcc gatgcctgcc
    10441 cggtgaagga ggaaacctac aaggtcgccg cacgccacgg cgtgagaacc atcgtggttt
    10501 ccaacacctg gatgaatgtg ccgcgcggcg atccgctgat cgaacaggtg gtcgtgcctg
    10561 cgggtctcga tgtcgcggat gactggatcg cggagcgggc ccgacgcggc gtcgtggtga
    10621 tcacggcgga cataccgctg gccgcacgca gcctcagggc gggggcggat gccatcgcgc
    10681 cgaacggccg gcccttcacc gagcagtcta tcggcaacgt gctcgcgaca cgcaacctga
    10741 tggatgacct gcgctccgca ggggcgatca ccggcgggcc gaaacccttc gcgccgaaag
    10801 accgttcggc gttcctgtca gcgctcgacc tggcgatcgt acggctgaag cgggccggat
    10861 tcggcggcgc ggcgcaagtc gcgccttgaa tcgctgcgaa gcgcgacacc accgcaatcg
    10921 cagcgctcag gacttcttcg cggcctttct cgcaggcttc tttgccggag ccttcgcttt
    10981 ggtgcctccg ccgctggctt cgcgggcgcg cagcagatcg attgcttgtt ccagcgtgat
    11041 atcgtccgcc gccatcgcct tcggaatgtt ggcgttgacc ttgccccagt tgacgtacgg
    11101 ac
//
LOCUS       DAOMXN010000061        11069 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_3174_length_11069_cov_12.114945, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000061 DAOMXN010000000
VERSION     DAOMXN010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 11069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11069
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3174_length_11069_cov_12.114945"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="PK812_06365"
     CDS             complement(<1..574)
                     /locus_tag="PK812_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003602995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HRJ69215.1"
                     /translation="MIPRYSRPAMTAIWEPQNRFRIWFEIEAHATDKLAELGVVPKEA
                     AAKIWEKGKDAVFDVARIDEIERVTKHDVIAFLTHLSEIVGEEARFVHQGMTSSDVLD
                     TCLNIQLTQAADLLIADLDALLAAIRRRAFEHKMTPTIGRSHGIHAEPVTFGLKLAQA
                     HAEFSRNRARLVAARAEVATCAISGAVGTFA"
     gene            complement(625..1290)
                     /gene="rpe"
                     /locus_tag="PK812_06370"
     CDS             complement(625..1290)
                     /gene="rpe"
                     /locus_tag="PK812_06370"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRJ69216.1"
                     /translation="MLRDLLIAPSILAADFASLGPDVAAADAAGGDWIHIDVMDGHFV
                     PNITFGPDLVKAIRPYSKKCFDVHLMIAPVDPYVEAFAKAGADLISFHVEASAHSHRT
                     LQLIRSLGKKSGIVLCPGTPASAIEPLLDMLDLVLVMSVNPGFGGQSFIKSVLPTIAA
                     CKALIGSRPIDLEVDGGITPETAPLAARAGANVLVAGSAFFRGGKEKYAENCRALRAA
                     AEL"
     gene            1421..2665
                     /locus_tag="PK812_06375"
     CDS             1421..2665
                     /locus_tag="PK812_06375"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009494122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRJ69217.1"
                     /translation="MRSDVLVLGAGFVGIGLALNLQAKGRSVTLVDRRGPGEETSYGN
                     TGLIQSEAVMPYPFPTNPWVILQAALNLRTDAYYSPSSLPKVASFIYGYWREAAKDRV
                     MRTARARAPIFEISIPEHEKLAQAAGAIAQFRPTGWLKVFRNDRNLADEIGKITELKQ
                     WNVEAAVLNHREVAAAEPGLTPGEIIGGVKYNGPISLAEPLALSRAYVELFKKQGGRV
                     VTGDARSLQQEGDGWSVAVEGGRESARDCVIALGPWAKDVTEPLGYRMPLGVKRGYHM
                     HFSARGNASLNGPILDGDVGYCLTPMTQGFRLTTGADFSDRDAPPVYVQVDRAEKAAR
                     GIFPLDSRLEDTPWMGRRPCTPDMIPVMGPAPRHKRLWFNFAHAHHGLTLAGSCGRFM
                     AEMICGETTYCDPKPYSAERFG"
     gene            complement(2668..3654)
                     /locus_tag="PK812_06380"
     CDS             complement(2668..3654)
                     /locus_tag="PK812_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HRJ69218.1"
                     /translation="MRNLITDVPGVSVGNVEDEKVATGVTAVIFDKTAVCSVRHGGGA
                     PGSRDTTLLETDMTQQGIDAVVLSGGSLFGLDAAGGVLSHLREQGRGFMLAGITIPIV
                     SQSIVFDLVNGGDKAWGHKPPYWNLGIEAARRAGKDFALGSAGGGYGATTANLKGGLG
                     SASMVTASGYTVGAIVIVNAIGMATIGDTPHFWAAPYEQNREFGGLGWPAAIPDAALA
                     LRVKGDAPATTIALVATDALLTKAEAKRIAIMADDGLGKAIRPAHAAFDGDTIYAAST
                     GRKVMANPMRDLTEIGLAAGDCLARAIARGVYEATALPFPGALPAWKDRFGP"
     gene            3742..>4472
                     /locus_tag="PK812_06385"
     CDS             3742..>4472
                     /locus_tag="PK812_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020179251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HRJ69219.1"
                     /translation="MNGDLTYPYVALAGLLSFLSPCVLPLVPPYLCYLAGTTLDEWSD
                     ETESGINHWALAAAACFVLGFSTVFVLLGAGASVIGGFLRQNSLEIGWIAGALIVVMG
                     LHFIGLIRIPILAREARLHVEKPAGVAGAYVMGLAFAFGWSPCIGPVLGSVFAVAGSE
                     ASVTRGMTLLAVYSAGLGIPFLIAAFAMGPFVRFLKRFRTHLGTVEKVMGGFLVATGI
                     AFITGSFTTLGTWLLDLFPALGKLG"
     assembly_gap    4473..4572
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4597..5712)
                     /locus_tag="PK812_06390"
     CDS             complement(4597..5712)
                     /locus_tag="PK812_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HRJ69220.1"
                     /translation="MKKTLLAGVAMTAALLLSGAAQAQIKMGVAGPITGPNAAFGKQL
                     TDGVEQAVEDINAAGGIMGQKLSVSKGDDVSDPKQGVSVANKFVGDGIKFVVGHFNSG
                     VTIPASEIYAENGILMISPSATNPKVTERGLWNAFRTCGRDDQQGEVAGAYIADKLKG
                     KKVAVVHDKTTYGQGLADETRKAMGKRGVKEVLYEGVNTGEKDFSALVSKMKAAGVEV
                     VYWGGLHTEGGLIIRQMRDQGLNAVMMSGDGITSAEFATIGGPGTEGTLMTFPPDPRA
                     RPEAKAIVEKYKAKKIDPESYTLYSYAAVEVIKQAAETAKSVDPKKVAEQIKSGQKFK
                     TVIGELSYDKKGDITRPDYVMYTWKKGPDGKISYFQQ"
     gene            complement(5903..6253)
                     /locus_tag="PK812_06395"
     CDS             complement(5903..6253)
                     /locus_tag="PK812_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69221.1"
                     /translation="MQGVLYEESSAWLFILVTIIMGGWAAWQTGQAVARTWKPRWQLI
                     FYMILLGWAVRFIHFALFEGTFLSLRYYIIDTLILMAFTFAGWKVQRARQMLRQYEWL
                     FDAAGPGAWKRKAQ"
     gene            complement(6253..6981)
                     /locus_tag="PK812_06400"
     CDS             complement(6253..6981)
                     /locus_tag="PK812_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ69222.1"
                     /translation="MSAQPILSVRGVKTYYGNIIALKGVDLDVNRGEIVTLIGANGAG
                     KSTLMMTIFGKPRAREGSIAYEGRDITQMPTHEISRLGISQSPEGRRIFPRMTVYENL
                     QMGAAVDGFRHFDADLERVTTIFPRLKERLSQRGGTLSGGEQQMLSIARALMARPKLL
                     LLDEPSLGLAPIIVRQIFDVIREINQKDGMTVFLVEQNAYHALKLAHRGYVMVNGVIT
                     MSGEGKKLLADPQVRAAYLEGGRH"
     gene            complement(6978..7889)
                     /locus_tag="PK812_06405"
     CDS             complement(6978..7889)
                     /locus_tag="PK812_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006935271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ69223.1"
                     /translation="MSAWQTTPLLKVEHLTMRFGGLVAINDLSFNVRKGDITALIGPN
                     GAGKTTVFNCITGFYKPTVGRLALRHGSVDLEGNVEAATDNAGPVTAVEHGSVFLLER
                     MPDFEITQQAKVARTFQNIRLFSGMTVLENLMVAQHNVLNAASTYTVGGLLGLRSFRD
                     AERQGVEKAKFWLEKTGLIDRADDPAGDLSYGAQRRLEIARAMCTDPVLLCLDEPAAG
                     LNPKESLELNTLIRSIRDQHECSVLLIEHDMSVVMEISDHVVVLDYGTKIADGTPKEI
                     QQDPKVIAAYLGVEDEEVDAVEAEVGL"
     gene            complement(7889..9259)
                     /gene="livM"
                     /locus_tag="PK812_06410"
     CDS             complement(7889..9259)
                     /gene="livM"
                     /locus_tag="PK812_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivM"
                     /protein_id="HRJ69224.1"
                     /translation="MAGVVDPDKEKSLTDILRDAVMATAVAFGMFVLLIGLKTDQSQA
                     NSIELSARPGLLGSILLAVFIGTVLIGIIRQQAAKRPAKPANAPPHPLVAALSPYLAP
                     FLIGFVFLFPIIAIQLAGWQGALKWIDNFGIQILIYIMLGWGLNVVVGLAGLLDLGYV
                     AFYAVGAYSYALLATKYGLSFWILLPIAGILAAFWGIILGFPVLRLRGDYLAVVTLAF
                     GEIIRLVLINWVDFSNGYAGISGIPRPTFFGIPFNASDTGFAAVFGLEFTPVYRTIFL
                     FYLILVLSLITAYVTVRLRRLPIGRAWEALREDEIACRALGINTVTTKLTAFAIGAMF
                     GGFAGSFFAARQGFISPESFTFIESAQILAIVVLGGLGSLYGVVIASVAIVGGFEILR
                     ELDFLKQVFGKDFDPAQYRALIFGLSMVLIMLWKPRGLVSTREPSVFLKERKAVSGSL
                     VKEGHG"
     gene            complement(9259..10182)
                     /locus_tag="PK812_06415"
     CDS             complement(9259..10182)
                     /locus_tag="PK812_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892483.1"
                     /note="LivHMGF is the membrane component of the LIV-I/LS
                     branched-chain amino acid transporter; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease LivH"
                     /protein_id="HRJ69225.1"
                     /translation="MEVFVQQVINGLTLGAIYGLIAVGYTMVFGIIGMVNFAHGDIFM
                     VGGFTALILFLLVTGALGFGAGAVALALLVCLILGMALTGLWNWAIEKVAYRPLRGSF
                     RLAPLISAIGMSIVLVNFVQVAQGARNKPVPPMLRDGIKLMEKDGFVVTLAYKQIVIF
                     VVTALLLGIFWYVVQKTPLGRAQRACEQDGKMASLLGIDVDRTVSITFVIGAALAAVA
                     GVMYLIYYGVISFSDGFVPGVKAFTAAVLGGIGSLPGAVLGGLIIGMVETLWSAYFSI
                     DYKDVAAFMLLAVTLIFLPQGLLGKPEVEKV"
     gene            complement(10340..>11069)
                     /locus_tag="PK812_06420"
     CDS             complement(10340..>11069)
                     /locus_tag="PK812_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014131201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HRJ69226.1"
                     /translation="LKPMSQVGGRMAVHPGASALEKSRGGRGVLIGGVPGVAPAKVVI
                     IGGGVGGFNAAQMAVGMHADVIILDRNPMVLEKLSTHFEASAKVVYAMKSAIEHHVEQ
                     ADLVIGAVLVPGAAAPKLVTRQMVKGMKKGAVLVDVAIDQGGCFETSRATTHAKPTYV
                     VDGVVHYCVANMPGGVARTSTYALNNVTLQHALAIADKGWKKALQTNHHLANGLNVCD
                     GKVTCEPVARDLGYAYVPVADMLR"
BASE COUNT         1908 a         3504 c         3632 g         1925 t
ORIGIN      
        1 tggcgaaggt gccgacggcg ccggagatgg cgcaggtcgc gacttccgca cgggccgcga
       61 cgaggcgggc gcggttgcgc gaaaactcgg catgggcctg cgccagcttc aggccgaagg
      121 tcaccggctc ggcatggatg ccatgcgagc ggccgatcgt cggcgtcatc ttgtgctcga
      181 aggcgcggcg cctgatggcg gcgagcaggg cgtcgaggtc ggcgatgaga agatcggcgg
      241 cctgggtgag ctggatgttc aggcaggtgt cgagcacgtc cgacgaggtc atgccctgat
      301 ggacgaagcg cgcttcctcg ccgacgatct cggacagatg cgtcaggaag gcgatgacgt
      361 catgcttggt aacgcgttca atctcgtcga tgcgggcgac gtcgaagacg gcgtccttgc
      421 ccttttccca gattttcgcg gcggcttcct tcggcacgac cccgagctcc gcgagcttgt
      481 cggttgcgtg cgcctcgatc tcgaaccaga tgcggaagcg gttctgcggc tcccagatcg
      541 cggtcatcgc agggcgggaa taacggggga tcatggggcg cttccttcgt acttctggac
      601 ggcccccctc attcggatga ggggttagag ttccgccgcc gcccgcaggg cacggcaatt
      661 ctcggcgtat ttctctttcc cgccccggaa aaaggcggaa ccggcgacga gcacgttggc
      721 gccggccctg gccgcgagcg gcgcggtttc cggcgtgatg ccgccgtcga cctccagatc
      781 gatgggccgg gagccgatca gcgccttgca ggcggcgatc gtcggcagga cggacttgat
      841 gaagctctgg ccgccgaagc ccgggttcac gctcatcacc agcacgagat cgagcatgtc
      901 gagcagcggt tcgatggccg aggccggcgt tccggggcaa aggacgatgc cggacttctt
      961 gccgagggag cggatgagct gcagcgtgcg atggctgtgc gcgctggcct cgacatggaa
     1021 ggagatgagg tccgcgccgg ccttcgcgaa ggcttcgacg taagggtcga ccggcgcgat
     1081 catcagatgc acgtcgaagc atttcttcga ataggggcga atcgccttca cgagatccgg
     1141 cccgaaggtg atgttcggca cgaaatggcc gtccatcacg tcgatatgga tccagtcacc
     1201 gccggcggcg tcggcggcgg cgacatccgg gccgagactg gcgaaatcgg cggcgaggat
     1261 ggacggggcg atgagcagat cgcggagcat gcgcgccgtt agcaccggac ggggccggga
     1321 acaaccacag catttgcatg gccgccattg atccaccggc ttgaaaggcg cgcgcggggg
     1381 cttcaaagtg cgtgccggtc aattcgggga tgtgtcggtc atgcgcagcg acgtgctggt
     1441 tcttggagcg gggttcgtgg gcatcggtct ggccctcaac ctccaggcga aagggcgcag
     1501 cgtcacgctc gtcgaccggc gcggaccagg cgaggagacc tcctacggca acaccgggct
     1561 gatccagtcg gaagctgtta tgccgtatcc gttcccgacc aatccctggg tcatcctcca
     1621 ggcggcgctg aacctgcgca cggatgccta ttacagcccg tcgtctttgc cgaaggttgc
     1681 ctccttcatc tacggctact ggcgcgaggc ggcgaaggac cgggtgatgc gcacggcgag
     1741 ggcgcgggcg ccgatcttcg agatttcgat tcccgaacac gagaagctgg cgcaggcggc
     1801 gggggcgatc gcgcagttcc gcccgaccgg ttggctcaag gtgttccgca acgaccgcaa
     1861 tctcgccgac gagatcggca agatcaccga actgaagcag tggaacgtcg aggccgcggt
     1921 gctcaaccac cgggaagtcg ccgccgccga gccgggtctg acaccgggcg agatcatcgg
     1981 cggcgtgaag tacaacgggc cgatcagcct ggccgagccg ctggcgctga gccgcgccta
     2041 tgtggagctg ttcaagaagc aggggggccg ggtcgtcacg ggcgacgcgc gctctctcca
     2101 gcaggagggc gacggctggt ccgtggcggt cgagggtggt cgggagagcg cgcgcgattg
     2161 cgtgattgcg ctcggcccct gggccaagga tgtcaccgag ccgctggggt atcgcatgcc
     2221 gctcggcgtc aagcgcggct atcacatgca tttcagcgcc agggggaatg ccagcctcaa
     2281 cggtccgatc ctcgacggcg acgttggtta ttgcctgaca ccgatgacgc aaggcttccg
     2341 cctgaccacc ggcgcggact tctccgatcg cgatgcgccg ccggtgtatg tacaggtgga
     2401 tcgcgcggaa aaggccgcgc gcggcatctt cccgctcgat tcacgccttg aggacacgcc
     2461 ctggatgggg cggcgaccgt gcacgccgga catgattccg gtgatgggcc cggcgccgcg
     2521 ccacaagcgg ctgtggttca acttcgctca tgcccaccac gggttgacgc tggcgggaag
     2581 ctgcggacgc ttcatggccg aaatgatctg cggcgagacg acctactgcg atccgaagcc
     2641 ctattcggcg gaacggttcg gctgaagtca gggcccgaac cggtccttcc acgctggcag
     2701 ggcaccgggg aagggcaggg ccgtcgcttc atacacgccg cgcgcgatgg cgcgggccag
     2761 gcaatcgcct gcggcaagcc cgatctcggt gagatcgcgc atcgggttgg ccatcacttt
     2821 gcggccggtc gaggcggcat agatcgtgtc gccgtcgaag gccgcatggg ccgggcggat
     2881 ggccttgccg agtccgtcat cggccatgat ggcgatgcgc ttggcttccg ccttggtgag
     2941 gagcgcatcg gtcgcgacaa gggcgatggt ggtcgccggc gcgtcgccct tgacgcgcag
     3001 ggccagcgcc gcgtccggga tggctgccgg ccagccgaga ccgccgaact cgcgattttg
     3061 ttcgtagggc gcggcccaga aatgcggtgt gtcgccaata gtcgccatgc cgatggcgtt
     3121 gacaatgacg atggcgccca ccgtgtatcc ggatgccgtg accatgctgg cagagccaag
     3181 cccgcccttg agattggcgg ttgtcgcgcc atatccccct cccgcggagc caagcgcgaa
     3241 atcctttccg gcgcgccggg cggcttcgat gcccagattc cagtagggcg gcttgtgacc
     3301 ccaggccttg tcgccgccgt tgaccagatc gaagacgatg gactgcgaga cgatcggaat
     3361 cgtgatgccg gccagcatga agccgcgccc ctgctctcgg agatgcgaca gaacgccgcc
     3421 cgccgcatcg aggccgaaca gggatccgcc ggacaggaca accgcatcga tgccctgctg
     3481 ggtcatgtcg gtttccagca gcgtggtgtc acgcgagcct ggcgcgccgc cgccatggcg
     3541 gaccgagcag actgccgtct tgtcgaagat cacggcggtg acgccggtcg cgaccttctc
     3601 gtcctcgaca ttgccgacgc tgacacccgg aacgtccgtg atcagattgc gcatgcgccc
     3661 tcgcgaaagt tttaggaggt tgagagtatt tctcatgcgc ggcatggcaa agtgcaaccg
     3721 gttccaaccc acacggaaac gatgaacggc gatctgacct atccctacgt ggccctcgcc
     3781 gggctcctga gcttcctcag cccctgcgtg ctgccgcttg tgccgccata cctgtgctat
     3841 ctcgccggca cgacgctgga cgagtggtca gacgaaacgg aaagcgggat caatcactgg
     3901 gcgctggcgg ctgcggcctg cttcgtactg gggttttcca cggtgttcgt gctgctcggc
     3961 gccggtgctt cggtgatcgg cggcttcctg cgacagaatt cgctggagat cggctggatc
     4021 gcaggggcgc tgatcgtcgt gatgggattg cacttcatcg gcctgatccg cattccgatc
     4081 ctggcgcgtg aggccaggct gcatgtggaa aagccggcgg gcgtggccgg ggcctatgtc
     4141 atgggtctcg ccttcgcctt cggctggtcc ccctgcatcg gccccgtcct tggcagcgtc
     4201 ttcgcggtgg ccggctcgga agcgagcgtg acgcggggca tgacgctgct ggcggtctat
     4261 tccgccggcc tgggcatacc gttcctgatc gcagcctttg cgatggggcc gttcgtgcgt
     4321 ttcctgaagc ggttccgcac ccatctcggc acggtcgaga aggtgatggg aggattcctg
     4381 gtggcgaccg gcatcgcgtt catcacggga tcgttcacga cgctcggaac ctggctgctt
     4441 gacttgtttc ctgccctggg aaagctcgga tannnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnctggcctg actggccggc gtcagcttac tgctggaagt agctgatctt
     4621 gccgtccggg cccttcttcc aggtgtacat cacgtagtcc ggacgggtga tgtcgccctt
     4681 cttgtcgtag gagagctcgc cgatcacggt cttgaacttc tgaccggact tgatctgctc
     4741 ggcgaccttc ttcgggtcga ccgacttggc ggtttccgcc gcctgcttga tcacctcgac
     4801 cgcggcgtag gagtagagcg tgtagctctc cggatcgatc ttcttggcct tgtacttctc
     4861 gacgatggcc ttggcttccg gacgggcgcg cgggtccggc gggaaggtca tcagcgtgcc
     4921 ttcggtgccc gggccgccga tggtggcgaa ctcggccgag gtgatgccgt cacccgacat
     4981 catcaccgcg ttcaggccct gatcgcgcat ctgacggatg atcaggccgc cttcggtgtg
     5041 caggccgccc cagtagacga cctcaacgcc ggccgccttc atcttggaaa ccagggccga
     5101 gaagtccttc tcgccggtgt tcacgccttc gtagaggact tccttgacgc cgcgcttgcc
     5161 catggccttg cgggtctcgt cggcgaggcc ctggccgtag gtggtcttgt cgtgcacgac
     5221 ggcgaccttc ttgcccttca gcttgtcggc gatgtaggcg ccggcaactt caccctgctg
     5281 atcgtcgcgg ccgcaggtgc ggaacgcgtt ccacaggccg cgctcggtga ccttcgggtt
     5341 ggtcgcggag gggctgatca tcaggatgcc gttctcggcg tagatttccg aggccgggat
     5401 ggtgacgccc gagttgaagt gaccgaccac gaacttgatg ccgtcgccga cgaacttgtt
     5461 ggcgaccgaa acgccctgct tgggatcgga aacgtcgtcg cctttcgaga cgctgagctt
     5521 ctggcccatg atgccgccag ccgcgttgat gtcctcaacc gcctgttcga caccgtccgt
     5581 gagctgcttg ccgaatgcgg cgttcgggcc ggtgatcggt ccggcgacgc ccatcttgat
     5641 ctgtgcctgc gctgctcccg agagcaggag ggcagccgtc atcgccacgc ctgccaaaag
     5701 agtctttttc atccaagtct cctcacttgc ttgaactggc ggggtactcc gcccccgcat
     5761 gcggttcggg cgcttacgag cgccttgttc cgatagaagc cggattccgg cgtgaagtca
     5821 tctgatttgt gcaggaagcg gtccggttcc gcccgctttc cgacctggcg ggatttgcgc
     5881 gcgtgcgtac cgcaggcgcc gttcattgcg ccttccgttt ccaggcaccc ggtccggcgg
     5941 catcgaacag ccactcgtac tgtcggagca tctgcctggc gcgctgcacc ttccagccgg
     6001 cgaaggtgaa ggccatgagt atcagcgtat cgatgatgta gtagcgcagg ctgaggaagg
     6061 tgccctcgaa caaggcgaaa tggatgaaac gcaccgccca tcccagcagg atcatgtaga
     6121 aaatcagctg ccagcgcggc ttccaggtgc gggcgacggc ctgcccggtc tgccaggccg
     6181 cccagccgcc catgatgatg gtcaccagga tgaacagcca ggcggagctt tcctcgtaga
     6241 gaacgccctg catcagtgcc tcccgccttc aagataggcc gcgcggacct gcggatcggc
     6301 cagaagcttc ttgccctcgc cggacatggt gatgacgccg ttgaccatga cgtagccgcg
     6361 atgggcaagt ttcagggcgt gataggcgtt ctgctccacc agaaacacgg tcatgccgtc
     6421 cttctggttg atttcccgga tcacatcgaa gatctggcgc acgatgatcg gcgccaggcc
     6481 gagggacggc tcgtccagca gcagcagctt tggccgcgcc atcagcgcgc gggcgatgga
     6541 cagcatctgc tgctcgccgc cggaaagcgt gccgccgcgc tgggaaagac gctccttcag
     6601 gcgcgggaag attgtcgtca cccgctccag gtccgcatcg aagtgacgga acccgtcgac
     6661 cgccgcgccc atctgcaggt tctcgtagac ggtcatgcgc gggaagatgc gtcggccttc
     6721 cggtgactgg ctgatgccga gccgcgagat ctcgtgggtc ggcatctgcg tgatgtcgcg
     6781 gccctcatag gctatgctgc cctcgcgggc gcgcggcttg ccaaagatgg tcatcatcaa
     6841 cgtcgacttg ccggcgccgt tggccccgat cagcgtgaca atctcgccgc gattgacgtc
     6901 gaggtccacg cccttcagcg cgatgatgtt gccgtaatag gtcttgacgc cgcgaacgga
     6961 caggatgggc tgcgcgctca taggcccacc tccgcttcca ccgcgtcgac ttcctcgtcc
     7021 tcgacgccga ggtaggcggc gatgaccttg ggatcctgct ggatttcctt cggcgtcccg
     7081 tcggcgatct tggtgccgta atccagcacc acgacgtggt cggaaatctc catcaccacc
     7141 gacatatcat gctcgatcag cagcacggag cactcgtgct ggtcgcgaat cgaccggatc
     7201 aacgtgttca gctcaaggct ctccttgggg ttcaggccgg ccgcgggctc gtcgaggcac
     7261 agcagcaccg ggtccgtgca catcgcccgc gcgatctcca gccggcgctg ggcgccgtag
     7321 gacaggtcgc cggcgggatc gtcggcgcgg tcgatcaggc cggtcttctc cagccagaac
     7381 ttggccttct ccacgccctg ccgctcggca tcccggaagg atcgcagccc caagagcccg
     7441 cccacggtat aggtcgaggc ggcgttgagc acgttgtgct gcgcaaccat caggttttcc
     7501 agaacggtca tgccggagaa caggcggatg ttctggaagg tgcgcgccac cttcgcctgc
     7561 tgcgtgattt caaaatccgg catgcgctcc aacaggaaca ccgagccgtg ctcaaccgcg
     7621 gtgacagggc cggcattgtc ggtcgcggcc tccacattgc cttcgaggtc gaccgacccg
     7681 tggcggaggg caagccggcc gaccgtcggc ttgtagaagc cggtgatgca gttgaagacg
     7741 gtggtcttgc cggcgccgtt cgggccgatc agggcggtga tgtcgccttt gcgaacgttg
     7801 aacgacagat cgttgatcgc gacgaggccg ccgaagcgca tcgtcagatg ttcgaccttg
     7861 aggaggggcg ttgtctgcca ggcgctcatc agccgtgtcc ctccttgacg agggacccgg
     7921 agaccgcctt gcgctccttg aggaagacgc ttggctcgcg ggtcgagacc aggccgcgcg
     7981 gtttccacag catgatcagc accatggaga gaccgaagat cagggcgcga tactgcgccg
     8041 gatcgaaatc cttgccgaag acctgcttga ggaaatcaag ctcgcgcagg atttcaaagc
     8101 cgccgacgat ggcgaccgag gcgatcacca cgccgtacag gctgccaagc ccccccagca
     8161 cgacgatggc gaggatctgc gccgactcga tgaaggtgaa gctctccggg ctgatgaagc
     8221 cctgacgcgc cgcgaagaac gagccggcga acccgccgaa catggcgccg atggcgaaag
     8281 cggtgagctt ggtcgtcacc gtgttgatgc ccagcgcccg gcaggcgatc tcgtcctcgc
     8341 gcaaggcttc ccaggcccgg ccgatgggca gccggcgcag ccggaccgtg acataggcgg
     8401 tgatcagcga cagcacgagg atgagataga acaggaagat cgtgcggtag accggcgtga
     8461 attccagccc gaacaccgcc gcgaagccgg tgtcgctggc attgaacggg atgccgaaga
     8521 aggtggggcg cggaatgccc gaaatgccgg cgtagccgtt ggagaaatcc acccagttga
     8581 tcagcaccag tcggatgatc tcgccgaaag ccagcgtcac cacggccagg tagtcgccgc
     8641 gcaggcgcaa gaccgggaag cccaggatga tgccccagaa ggcggcaaga attccggcga
     8701 tgggcaggag gatccagaag gacaggccgt atttcgtcgc cagcagcgca taggagtagg
     8761 cgcccacggc atagaacgcg acgtagccga ggtccagcag gccggcgagg ccgacaacca
     8821 cgttcagccc ccagcccagc atgatgtaga tcaggatctg gatgccgaag ttgtcgatcc
     8881 acttcagggc gccctgccag cccgcgagct ggatcgcgat gatcgggaac agaaagacga
     8941 agccgatcag gaatggcgcg agatagggcg aaagcgccgc gaccagcgga tgcggcggcg
     9001 cgttcgccgg ctttgccggt cgtttcgccg cctgctgccg gatgatgccg atcagcacgg
     9061 tgccgatgaa cacggccagc aagatcgatc ccaggaggcc aggacgggcc gaaagctcga
     9121 tcgaattcgc ctggctctga tccgtcttca gaccgatcag caacacgaac atgccgaagg
     9181 cgacggctgt cgccatgacc gcatcgcgca ggatgtcggt gagcgacttt tccttgtccg
     9241 gatcgacgac gccagccatc acaccttctc cacttccggc ttgccgagca ggccctgggg
     9301 caggaagatc agtgtcacgg cgagcagcat gaaggccgca acatccttgt aatcaatgga
     9361 gaaataggcg gaccacagcg tctccaccat gccgatgatg aggccgccca gaaccgctcc
     9421 cggcagggaa ccgatgccgc ccagaaccgc cgccgtgaag gccttgacgc ccgggacgaa
     9481 gccgtccgag aacgagatca cgccgtagta gatcaggtac atcacgccgg cgaccgcggc
     9541 gagcgccgcg ccgatgacga aggtgatcga cacggtacgg tccacgtcga tgccgagcag
     9601 cgacgccatc ttgccgtcct gctcgcaggc gcgctgcgcc cggccgagcg gcgtcttctg
     9661 cacgacgtac cagaagatgc cgagcaacag ggcggtcacc acgaagatga cgatctgctt
     9721 gtaggcgagg gtgacgacaa acccgtcctt ttccatcagc ttgatgccgt cccgcagcat
     9781 cggcggcacc ggcttgttgc gcgcgccctg cgccacctgc acgaagttga cgagcacgat
     9841 ggacatgccg atggccgaga tgagcggcgc caggcggaag gagccgcgca agggccggta
     9901 ggccactttt tcgatggccc agttccacag cccggtcagc gccatgccga ggatgaggca
     9961 gaccagaagc gccagcgcga cggcgcccgc gccaaagccg agcgccccgg tgacaagcag
    10021 gaacaggatg agcgccgtga agccgcccac catgaaaatg tcgccgtggg cgaaattgac
    10081 catgccgatg atgccgaaga ccatcgtata tccgacggca atcaacccat agatcgcccc
    10141 gagcgtcagc ccgttgatga cctgctgaac aaaaacttcc atttgtcgcg taaccccgcc
    10201 ctggttcccg gcggatcact cccctccacc ggtctggcca ttcggcccgt ttatcgttct
    10261 tatcagcccg gctgatgcag ccaagccccc cgtcgtcact ttgtcacggt tgcctggaca
    10321 gccggctgcc gtctgctgct taccgcagca tgtcggcgac cggcacgtag gcgtacccga
    10381 ggtcgcgcgc aaccggctcg caggtgacct tgccatcgca gacattcagc ccgttggcga
    10441 gatgatggtt ggtctgcaac gccttcttcc agcccttgtc ggcgatggcg agcgcgtgct
    10501 gcagcgtcac gttgttcagg gcataggtcg aggtgcgggc gacgccgccc ggcatgttgg
    10561 cgacgcagta gtgcacgacc ccatcgacca cataggtcgg cttggcatgc gtggtcgcgc
    10621 gcgatgtctc gaagcagccg ccctgatcga tggcgacatc gaccagaacc gcgcccttct
    10681 tcatgccctt gaccatctga cgggtgacga gcttcggcgc ggccgcgccc ggcaccagca
    10741 ccgcgccgat gaccagatcg gcctgctcga cgtggtgttc tatggccgac ttcatggcgt
    10801 agacgacctt ggcactcgcc tcgaaatggg tcgacagctt ttccaggacc atcgggttgc
    10861 gatccaggat gatgacgtcc gcatgcatgc ccacggccat ctgtgcggcg ttgaagccgc
    10921 ccacgccgcc gccgatgatg accaccttcg ccggcgcgac gcccggcacg ccgccgatca
    10981 gcacgccgcg ccctccgcgc gacttctcca gcgccgacgc tccgggatgc acggccatgc
    11041 gaccgcccac ctggctcatc ggcttcagg
//
LOCUS       DAOMXN010000062        11016 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_3191_length_11016_cov_10.466563, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000062 DAOMXN010000000
VERSION     DAOMXN010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 11016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11016
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3191_length_11016_cov_10.466563"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1053)
                     /locus_tag="PK812_06425"
     CDS             complement(<1..1053)
                     /locus_tag="PK812_06425"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRJ69227.1"
                     /translation="MSGAQTYRDDIQGLRALAVVPVVLYHADDRLLPGGFVGVDIFFV
                     ISGFLITQILLREIANGTFSLRGFYERRVRRLFPALYAMLAATLIWGYMILSPADFRE
                     LALTALATVVFASNAMFLSLSDYFDGPAELKPLLHTWSLAVEEQFYLLFPLVLIAIAR
                     FAPRLLKPLLWIALILSLALCIRATRSFQAAAFYLPMTRAWELLAGAVLAAGAVPACR
                     TRLSRDVLSVLGLLLIAGSLLFIDKSMRFPGAFPLFPVLGAALVIHAGQGGESIGGRV
                     LSLAPLVWIGALSYSLYLWHWPPIAFARYTTIGEPDPARLWLAAAISLPLAVASLYWI
                     ERPMLKAKLSGRAILQG"
     gene            complement(1207..1596)
                     /locus_tag="PK812_06430"
     CDS             complement(1207..1596)
                     /locus_tag="PK812_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRJ69228.1"
                     /translation="MSRSQQFEKGLKVRRAVLGAEYVDGSIAKADDFMMAFQEITTEW
                     CWGYAWTRPGLDLKTRSMLNLAMLTALGKPAELRLHVKGALTNGVSVDEIKEILLHAT
                     VYSGIPAGLEAFKAAHEVLKAEGALEK"
     gene            complement(1692..2816)
                     /locus_tag="PK812_06435"
     CDS             complement(1692..2816)
                     /locus_tag="PK812_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRJ69229.1"
                     /translation="MGISNDGRGLSVSRRKLLGTAGLAAASTTVAMPAIAQSQPSLTW
                     RMPMFVPKNVESVNGAADDFIKRVSDLTDGRFKITKFGAGEITPGGPAVLDAAEQGTV
                     ECSFTLSYYSVGKDPAYAFGSTLPFGFNLRGQTAWLYKWGGLELCNEFHNARGTHGLP
                     VGNSTSQMGGFFRKEINKLDDLKGLKMRIPGLGGTVMSKLGVIPQQISPADIYPALER
                     GTIDAAEWAAPYDDFKFGLHKVAPYYYAPGWWEPQATIMVFVNKPKWDELTPQYKAVL
                     EAAASATWTSHMAWNDRLNSDGLRELVKAGAQVRFFSKEILDAAYEESFKLFDELAQR
                     SPAFAKIYPSWKKHLDTQELYYRIGDATYDNYIYAKRSRS"
     gene            complement(2984..4291)
                     /locus_tag="PK812_06440"
     CDS             complement(2984..4291)
                     /locus_tag="PK812_06440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HRJ69230.1"
                     /translation="MSRINEDFATDVRAAIMEKAGTRTWPLSIGAVGLLAIAAWWASV
                     AVLDEVTSGDGRVIPSSQTQVVQVLEGALISAVNVKVGDRVTTGQPLMRVNDVTAQSQ
                     LGELNEKREAFLARNARLSAEAQGKAPDFSGINAGNNLLAAEKALFESRKSSLDLEVM
                     AADQALAQRRQERVEADVRIRESDRTLGLLDRELTLARNLQTRGVFPEIDMVRLERQR
                     QGEARDQALLKASLSRLDQAIAETQARRDAAIQKFRATAQEEFAKTLGEMSVLEETLR
                     AAEERVRRTTLRAPVNGIVNAIPVTTIGAVVQPGKNIIEIVPVDDRLLIEARLRPQDI
                     AFVAPGQPASVKITAFDYTIYGDMKGRVERVGADTKFDDKGNPYYEVTVRTDSNRLGP
                     PERTLQIIPGMVTRVDIQTSRKTVMQYLLRPMARMKNEAMRER"
     gene            complement(4288..6474)
                     /locus_tag="PK812_06445"
     CDS             complement(4288..6474)
                     /locus_tag="PK812_06445"
                     /inference="COORDINATES: protein motif:HMM:TIGR03375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HRJ69231.1"
                     /translation="MNDTAPPPEPQSQTPVRPADGPPAIGLAGALLLAARHHGLGITA
                     NALTAGLTGDTGEIPLAALREAAEKAGLRVDLRRAGLGQVPGSVLPIIAFLPDGQAIF
                     VEKVDFSTGHVSGAYGSTGQPAELDPDAFGPSPPIVLIAPARPIGQGGQANEPAPSSA
                     WFWPFVGRFWPDYLQMAVAGFAINILGLALPLFTMNVYDRVIPNAATSTLWALFWGIV
                     IVLAFDLLLRSLRAWIVDEVGRRVDLAVSGRLFDHLLNLNLAGKPRSAGILASQIKEF
                     ESVREVMTSGAVIAVADAIFIVIFLAAMYLIVGPLVIVPMVAVPVVLLLTVLVQMPIS
                     RTMDETQAAAAQRHGTLVEAVVGLETIKIVGAQNVMRQRWDRAVAAASRAMSKARFWS
                     SLTTNILSTAQQTVTIALTVWGVYLIIDGQITMGALVAANLLSGRVLAPLAGIAQTLT
                     RAAQAKAALKGLSRFMSLPSETIAPEKATSLTPKKGAVIFQNVSFQYAGSPTKALDKV
                     SFSVKPGEKIGIIGRIGSGKTTFARLLAGLYAPTEGSVLIDNIDVRQYSPLDLRKFVG
                     VLPQDSDLFSGTLRDNIVIGRPTAGEGRLRQAVEMSGVNAFASAHPQGLAMPITERGA
                     GLSGGQRQSVSLARFLIREPRVMFLDEPTASMDTTTEAMLIQRLARIADTGVTMFIST
                     HRDSLLRIVDRLFVLDGGRLVMDGPRDEVLKALADRAARLSGAKPT"
     gene            complement(6477..7841)
                     /locus_tag="PK812_06450"
     CDS             complement(6477..7841)
                     /locus_tag="PK812_06450"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRJ69232.1"
                     /translation="MAIRAIALAMALALQGLPAGATSLDETVRRAVASYPSIPESAAS
                     RRSIEADFDGANAALAPRADLEAYAGPQMFDRPESLSNDLNQKWRFHRQAGLTVRHTL
                     FDAWARENEIYRQGMRTNSSAFRVLERTGQVAIEAVEAHIDVVRHSRVLALAEDNIAA
                     HQRILTDIQARFSGGGSGAGETEQAKERLAAAHAIRGEILRSVGAARARYKRLTGIEP
                     KRLAGPRSARLMPKSRQNALSTAYAHHPSLIAAGFDIKGVDAEVEQSKARAYPQIGVE
                     GKASVGQELGGVKSWNNEASARVTMSWTIFDGGVNDARRRASVEKRTETELRRDRMMR
                     EIAETVEIAWSDIGATSQRLDALRRQTAASERVVQVYRDEFNGGRRTLIDVLDAQNSL
                     FNTRVAAAGAEAITAYTRFKLVASMGRILNEFGVSAPRESLPLPSGAIGDIRRLGVPL
                     EPIR"
     gene            8022..9080
                     /locus_tag="PK812_06455"
     CDS             8022..9080
                     /locus_tag="PK812_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69233.1"
                     /translation="MTLPFKSAGARFLALFLLVAGGLAATLFLILALLDPYGVSPLRL
                     PISRPMMDINQRYMYPQVARSGRFDSAVFGTSTLRLLDPEKLDAKFGGAFANLAMNAA
                     TPWEQLQLASLMSRHTPGLRTVILGLDAPWCEADADALAKRTTFRAFPESFYDDSTVN
                     DLVHFFNLKTLEITGRLLGYHLGRATPRIRQDGYENFLPPETDWTPAYAERLLWTGVN
                     KPIVPVVPPYVLSPEEARTLRFPAIDWLGAFASALPAATRLILIFPPNHIAGQPIPGS
                     RNDARERTCREALLRKVGKRASIIDMKFANDLTRTDSNYWDSLHFRLPIADEVLAMIH
                     EAISTDAPGGAYWRRLRP"
     gene            9129..9437
                     /locus_tag="PK812_06460"
     CDS             9129..9437
                     /locus_tag="PK812_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69234.1"
                     /translation="MGIIVGFIALILAVGALVLAWPLGWLVGGYRRAVVLLSLLLFLA
                     GWAIVWVQPIEQSRPQTAIWPISVGGSGVLAVILMAYGHAFAWLWRLVARLLGRGQQT
                     "
     gene            9558..10496
                     /locus_tag="PK812_06465"
     CDS             9558..10496
                     /locus_tag="PK812_06465"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRJ69235.1"
                     /translation="MSSLKSLAFAALAAAALASGPALAQGKITVAHTASGDYASGFWA
                     VDKGLFKKRGLDVEFKLVPLNPQIPAAVQSGSVQVGGPTTTVLLQAVDGGLDHVALAG
                     SGLTRKNSDTFGTLARAEAPISGAKGFEGKKVGAPGIGAFLHVLFSKWLLDNGADLKK
                     VTFVEVAFPQHLDVLKGGTVDAIVTGEPFTSRILQAGVGKVSTHFTDNLTNDMPIIVY
                     SATRAWAQANPAAAKAFAEAIAEATEAVNNPANADEVRTVIGKFIQMPEPVLKTLKLG
                     TWKAEVTPAGLKDWIDTMKSQGLIQSNLDVAKIIWK"
     gene            10678..>11016
                     /locus_tag="PK812_06470"
     CDS             10678..>11016
                     /locus_tag="PK812_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRJ69236.1"
                     /translation="MTASIAFETTSIDLGGRTILDAIDFTVTPGTFVSIVGASGSGKT
                     TLLRLAAGLYLPTRGSVRIDGRVVSGPDPAVAIVFQDYGRALLPWRTAAGNVSLALEA
                     IGLPKARRPDR"
BASE COUNT         1891 a         3741 c         3503 g         1881 t
ORIGIN      
        1 gccctgcagg atggcgcgtc cggagagctt ggccttcagc atcggccgtt cgatccagta
       61 cagcgaggcc accgcgagcg gcagcgagat cgccgccgcc agccagagcc gcgcggggtc
      121 cggttcgccg atcgtggtgt agcgggcaaa ggcaatgggc ggccagtgcc agaggtagag
      181 cgaataggac agcgcgccga tccagacgag cggcgccagc gacagcaccc gcccgccgat
      241 gctctccccg ccctgcccgg catggatcac cagcgcggcg cccagcaccg ggaacagcgg
      301 aaatgcgccc ggaaaccgca tcgacttatc gatgaacagc aggctgccgg caatcagcag
      361 cagccccaga accgacagca cgtcccggct caggcgggtg cggcaggccg gaacggcgcc
      421 ggcggccagc accgcgccgg ccagcagttc ccaggcccgc gtcatcggca gatagaaggc
      481 ggcggcctga aagctgcgcg tcgcccggat gcacagggcc agcgacagga tgagcgcgat
      541 ccacagcagc ggtttcagca gccgcggcgc gaaacgggcg atggcgatca gcaccagcgg
      601 gaacagcagg tagaactgct cctccaccgc cagtgaccag gtgtgcagca gcggcttgag
      661 ttccgccggc ccgtcgaaat aatccgacaa cgacaggaac atcgcgttcg aggcaaagac
      721 caccgtggcc agcgccgtca gcgccagttc ccggaagtcg gccggcgaca ggatcatgta
      781 gccccagatc agcgtcgccg cgagcatggc atagagcgcc ggaaacagac ggcgcacccg
      841 ccgctcgtag aagccgcgca gggaaaacgt gccgttcgcg atctcgcgca gcaggatctg
      901 cgtgatcagg aagcccgaaa tgacaaagaa gatgtcgacg ccgacgaagc cgcccggcaa
      961 cagccggtcg tcggcgtgat acagcacgac gggcaccacc gcgagcgcgc gcagcccctg
     1021 aatgtcgtcc cggtatgtct gcgcgccgct caaatcccgt ctgcctgtcc gaagtgccag
     1081 gtacgacgtg tcgcacagaa gacttgacgc ttcccttccg tcagcgctcg cttttcaggc
     1141 atcgcatcga attcgcggac gcgttgaccg gccgttgaag aaacggcagc ccgcgcggtt
     1201 tcggcgctat ttttccagcg cgccctctgc cttcagcacc tcatgcgccg ccttgaaggc
     1261 ttcgagcccg gccgggatgc cggaatagac ggtagcgtgc agcaggattt ccttgatctc
     1321 atcgacgctg acgccgttgg tcagcgcgcc tttcacatgc aagcgcagtt cagcgggctt
     1381 gccgagcgcc gtgagcatgg cgaggttcag catggagcgg gtcttcaggt cgaggcccgg
     1441 ccgcgtccag gcatagcccc agcaccattc cgtggtgatc tcctgaaagg ccatcatgaa
     1501 atcgtcggcc ttggcgatag atccatcgac atactccgcg ccgagcacgg cgcggcggac
     1561 cttcaggcct ttttcgaact gctgcgagcg ggacatggcg actccttggt gatggagccg
     1621 ccagcggacc cgtcccgccg cgtcgtgtca acgcccaccg ggccccttcc gggcccggtc
     1681 cctcgacagg atcagctgcg cgagcgcttg gcgtagatgt agttgtcgta ggtcgcgtcg
     1741 ccgatccggt aatacagctc ctgcgtgtcg aggtgctttt tccacgaggg atagatcttg
     1801 gcgaaggccg gcgaccgctg cgccagttcg tcgaacagct tgaaggattc ctcgtaggcc
     1861 gcgtcgagga tttcctttga gaagaatcgc acctgcgcgc cggccttgac cagttcacgc
     1921 aggccgtcgg agttcagccg gtcgttccag gccatgtggc tggtccaggt ggcggaagcg
     1981 gcagcttcca gcaccgcctt gtactgcggg gtcagttcat cccatttcgg cttgttgacg
     2041 aagaccatga tggttgcctg cggctcccac cagcccggtg cataatagta aggtgccacc
     2101 ttgtgcaggc cgaacttgaa gtcgtcgtag ggagcagccc actccgccgc gtcgatcgtg
     2161 ccgcgctcca gcgccggata gatatccgcc ggagaaatct gctgcgggat gacgccgagc
     2221 ttgctcatca ccgtgccgcc gaggcccgga atgcgcatct tcagcccttt cagatcgtcg
     2281 agcttgttga tctccttgcg gaagaagccg cccatctgcg acgtggaatt gccgaccggc
     2341 agcccatgcg tgccacgtgc attgtggaat tcgttgcaca gttccagccc gccccacttg
     2401 tacagccagg ctgtctggcc gcgcaggttg aacccgaatg gcagcgtcga accgaacgcg
     2461 taggccggat ccttgcccac agaatagtag gacagcgtga acgagcactc gaccgtgccc
     2521 tgctcggccg catccagcac cgccgggccg ccgggcgtga tctcgccggc gccgaacttg
     2581 gtgatcttga accttccgtc ggtcaggtcg gaaacacgct tgatgaagtc gtccgccgcc
     2641 ccgttcacgc tttcgacgtt cttcggcacg aacatcggca tgcgccaggt caatgaaggc
     2701 tgcgactgcg cgatcgccgg catggcgacc gtggtggagg ccgcggccag tccggccgtg
     2761 cccaacagct tgcggcggct tacggacaag ccccgcccgt cattcgaaat gcccatggtt
     2821 ccctcctcgt cgcgcgcgtt tcgcgcttgt cgaacagcat gcttcaacat gccgatccaa
     2881 gacaaggcat accacataat gattatgttg tataaccatt tttcgggcgc gtcgccccat
     2941 cggtgcggcg cgcgactgaa atggacctgt gggcagcggc ggatcagcgc tcgcgcatcg
     3001 cctcgttctt catgcgcgcc atgggccgca gaagatattg catcaccgtc ttgcggctgg
     3061 tctgaatgtc gacgcgcgtc accatgcccg gaatgatctg cagggtgcgt tcgggcgggc
     3121 caaggcgatt ggaatcggtg cgcaccgtca cctcgtagta cggattgccc ttgtcatcga
     3181 acttggtgtc ggcaccgacg cgctcgaccc tgcccttcat gtcgccgtag atggtgtagt
     3241 cgaaggcagt gatcttcacg ctcgccggct gtcccggggc gacgaaggcg atgtcctgcg
     3301 gccgcagccg cgcttcgatc agcagtctat cgtcgaccgg gacgatctcg atgatgttct
     3361 tgccgggctg caccacggcg ccgatggtcg tcaccgggat ggcgttgacg atgccattca
     3421 cgggcgcgcg cagcgtggtc cgtcgcacgc gctcttccgc ggcgcgcagc gtttcttcca
     3481 gaaccgacat ctcgcccagg gtcttggcga attcctcctg cgccgtcgcc cggaacttct
     3541 ggatcgccgc gtcgcggcgg gcctgggtct cggcgatggc ctggtcgagt cgcgacagcg
     3601 aagccttgag cagggcctga tcgcgcgcct cgccctggcg ctggcgttcc agccgcacca
     3661 tgtcgatctc gggaaacacc ccgcgcgtct ggagattgcg agccagcgtc agctcgcgat
     3721 cgagcaggcc cagcgtgcgg tcgctctcgc gaatgcgtac atcagcctcg acgcgctcct
     3781 gccggcgctg cgccagcgcc tgatcggctg ccatcacttc gagatcgagc gaactcttgc
     3841 ggctctcgaa cagcgccttc tcggccgcga ggaggttgtt gccggcattg atgccggaaa
     3901 agtccggggc cttgccctgc gcctcggcgg acaaccgcgc attgcgcgcc agaaaggcct
     3961 cgcgcttctc gttcagttca ccgagctgag actgcgccgt gacgtcgttc acgcgcatca
     4021 gcggctgccc ggtcgtgacg cggtcaccga ccttcacgtt cacggctgaa atcagtgcgc
     4081 cttcgagcac ctgcaccact tgcgtctgcg atgacgggat gacgcggcca tcgccggagg
     4141 tcacctcatc cagcactgcg acggaagccc accaggcggc aatagcgagc agccccaccg
     4201 cgccgatcga cagcggccag gtccgggtgc cggccttttc catgatcgcg gcgcgcacat
     4261 cggtggcgaa atcctcgttg atccggctca tgtcggcttg gctcccgaaa gccgggccgc
     4321 ccgatcggca agcgccttca agacctcatc gcgcggtccg tccataacca gccggccgcc
     4381 gtcgagcacg aacagccggt cgacgatgcg cagcaacgag tcccgatggg tcgagatgaa
     4441 catcgtgacg ccggtgtcgg cgatgcgggc cagccgctgg atcagcatgg cttccgtggt
     4501 cgtgtccatc gacgctgtcg gctcgtccag gaacatgacg cgcggctcgc ggatcaggaa
     4561 gcgcgccagc gacacggact gcctctgccc gccggaaagg cccgcgccgc gctcggtgat
     4621 cggcatggcg aggccttgcg gatgcgcgga agcgaaggca ttgacacccg acatctcgac
     4681 tgcctgacgc agacgtccct ctcccgccgt cggtcgaccg atgacgatgt tgtcgcgcag
     4741 cgtgccgctg aacaggtccg aatcctgggg aagcacgccg acgaacttgc gcagatccaa
     4801 cggactgtat tggcgcacat cgatgttatc gatcagcacc gacccttcag tcggcgcgta
     4861 aagtccggcc agaagccgcg cgaacgtcgt tttgccggag ccgatgcggc cgatgattcc
     4921 gatcttctcg cccggcttga ccgagaaact caccttgtcc agcgccttcg tcgggcttcc
     4981 cgcatactgg aacgacacgt tctggaagat gaccgcgccc ttcttcggcg tcaggctggt
     5041 cgccttctcc ggcgcgatgg tctcgctcgg cagcgacatg aaccggctca gccccttgag
     5101 ggcggccttg gcctgtgcgg cgcgcgtcag ggtctgggcg attccggcca gcggcgcgag
     5161 aacacggccg gacaggaggt tcgccgccac cagcgcgccc atggtgatct gcccgtcgat
     5221 gatcagatag acgccccaga ccgtgagcgc gatggtcacg gtctgctgcg ccgtcgacag
     5281 gatgttggtg gtcaggctgg accagaaccg cgccttcgac atggcgcggc tggccgcggc
     5341 cactgcccgg tcccagcgct ggcgcatcac gttctgcgcg ccgacgatct tgatggtctc
     5401 aaggccgaca acggcctcca ccagcgtgcc gtggcgctgg gccgccgccg cctgggtttc
     5461 gtccatcgtg cgcgagatcg gcatctgcac gagcacggtc agcagcagca cgaccggcac
     5521 ggcaaccatc ggcacgatca ccagaggccc gacgatcagg tacatcgcgg ccaggaagat
     5581 gacaatgaag atggcatcgg cgacggcgat caccgcgccc gaggtcatga cttcgcgtac
     5641 gctctcgaat tccttgatct ggctggcaag gatgccggcc gagcgcggct tgccggcgag
     5701 gttgagattg agcagatggt cgaaaagacg gccggaaacc gccagatcta cgcgtcgccc
     5761 tacctcatcc acgatccacg cgcgcagcga tcgcagcagc agatcgaaag ccaggacgat
     5821 gacgatgccc cagaacagcg cccaaagcgt cgaggtggcc gcgttgggaa tgacgcggtc
     5881 gtagacgttc atcgtgaaaa gcggcagggc cagcccgaga atgttgatgg cgaagcccgc
     5941 caccgccatc tgcaggtagt ccggccagaa gcggccgacg aagggccaga accaggccga
     6001 ggacggcgcc ggctcgttgg cttgcccccc ctgcccgatc ggcctggccg gcgcgatcag
     6061 cacgattggc ggcgaaggcc cgaaggcgtc gggatccagc tccgccggct gtcccgtgga
     6121 gccgtaggcg ccggaaacgt ggccggttga gaaatcgacc ttctcgacaa agatcgcctg
     6181 tccgtctggc aggaaggcaa tgatcggcag aacgctgccg ggcacctggc cgaggccggc
     6241 cctgcgtaga tcaacacgca gcccggcctt ctccgccgcc tcgcgcaatg cggcgagcgg
     6301 gatctcgccg gtatcgccgg tcagaccggc ggtcagcgcg ttcgccgtga tcccaaggcc
     6361 gtgatggcgc gcggcaagaa gcagggcccc ggcaagaccg atggccgggg ggccatccgc
     6421 cggcctgacc ggagtttgcg actgtggttc aggtggcgga gctgtgtcgt tcaatgtcag
     6481 cgtatcggtt cgagcggcac acccagtcgg cggatgtcgc cgattgcgcc ggatggcagc
     6541 ggcagggact cccttggagc agatacgccg aattcgttaa gaatgcgccc cattgaggcg
     6601 accagcttga agcgcgtata cgccgtgatc gcctcggcgc cggccgccgc aacgcgcgtg
     6661 ttgaacaggg aattctgcgc gtcgagcaca tcgatgagcg tgcgccgccc gccattgaac
     6721 tcatcgcggt aaacctgcac gacgcgctcc gatgcggctg tctgtcgccg caacgcatcg
     6781 agtcgctgcg acgtcgcgcc gatgtccgac caggcgatct cgaccgtctc ggcgatctcg
     6841 cgcatcatcc ggtcgcgtcg caactcggtt tccgtgcgct tctcaaccga cgcgcggcgg
     6901 cgcgcatcgt tgacgccgcc atcgaaaatg gtccacgaca tggtcacgcg cgcggacgcc
     6961 tcgttgttcc agctcttcac gccgcccagc tcctggccga ccgaagcctt tccctccacg
     7021 ccgatttgcg gataggccct ggccttggac tgctcgacct cggcatcgac gcccttgatg
     7081 tcgaagcccg ccgcgatgag cgagggatgg tgcgcgtaag ccgtgctgag cgcgttctgc
     7141 cgggacttcg gcatcaggcg cgcgctccgc ggaccggcca gacgcttcgg ctcgataccg
     7201 gtcagccgct tgtagcgcgc cctggctgct ccaaccgagc gcaggatttc cccgcggatc
     7261 gcatgggctg ccgccagccg ttccttcgcc tgctccgtct cgccggcgcc ggaaccgccg
     7321 ccggaaaacc gtgcctgaat gtccgtcaga atgcgctggt gtgccgcgat gttatcctcg
     7381 gccagcgcca gcacgcggct gtgccgcacg acatcgatgt gcgcttccac cgcctcgatc
     7441 gccacctgcc ccgtgcgctc cagcacccgg aaggcggacg agttcgtccg catgccctgc
     7501 cggtaaatct cattctcgcg cgcccaggcg tcgaacagag tatgacgcac cgtcaggccg
     7561 gcctgccggt gaaagcgcca cttctggttt agatcgttcg acagggattc cggcctgtcg
     7621 aacatctgcg gcccggcata ggcctccaga tcagcgcgcg gcgccagggc agcattggct
     7681 ccgtcgaaat cagcctcgat ggaccgccgt gaagccgccg attccggaat ggagggatag
     7741 ctcgcaaccg cacggcgaac ggtctcgtcc agcgaggtcg cgcccgccgg cagtccctgt
     7801 agggcgagcg ccatggcaag cgcaatcgca cgaatcgcca ttgatttcat cagctgtcca
     7861 ccaggacgag ttcctaacag tgcgttaggg acttgccaag caacccgctc gcaagtgccc
     7921 cggcattcac gaaaagaacg ggcttccacc cggacccgtt gccgggaaga cacggcccgg
     7981 acgcattgcc gcccgctctc agggcgggta tggaccgctc catgacgttg ccattcaagt
     8041 ccgccggcgc acgttttctc gccctgttcc tactggtcgc gggcggactg gcggcgaccc
     8101 ttttcctgat cctggcgctt ctcgaccctt acggcgtctc gccactgcgc ttgccgattt
     8161 cccgaccgat gatggacatc aaccagcgct acatgtaccc gcaggtggcg cgctcgggcc
     8221 ggttcgactc cgcggttttc ggcacctcga ccttgcggct gctcgatccg gaaaagctgg
     8281 acgccaagtt cggcggcgcg tttgccaatc tcgccatgaa tgccgcgacg ccctgggaac
     8341 agttgcagct cgcgtccctc atgagccgcc atacgcccgg cctgcgcacc gtgatccttg
     8401 gccttgatgc cccctggtgc gaagccgacg ccgacgcgct ggccaagcgc accaccttcc
     8461 gggcatttcc cgagagtttc tatgatgaca gcacggtcaa tgacctggtg catttcttca
     8521 atctgaagac gctggagatc accggccggc tgctcggcta tcacctcggc cgcgccacgc
     8581 cgcgtatccg gcaggatggt tatgagaatt ttctgccccc ggaaacggac tggactccgg
     8641 cctatgccga gcgcctgctg tggaccggcg tgaacaagcc catcgtgcca gttgtgcccc
     8701 cttacgtgct ttcgccggaa gaagcgcgga ccttgcggtt ccccgccatc gattggctcg
     8761 gcgcctttgc cagcgcgctg ccggccgcca cccggctcat cctgatcttc ccacccaatc
     8821 acatcgcggg acaaccgatc cccggctcgc gcaacgacgc gcgcgaacgg acctgccgtg
     8881 aggccctcct gcgcaaagtg gggaagcggg cgagcatcat cgacatgaag ttcgccaatg
     8941 acctgacacg caccgattcc aactactggg acagtctgca tttccgcctg ccgatcgccg
     9001 acgaagttct cgcgatgatc cacgaggcga tcagcacgga tgcgccgggc ggagcgtact
     9061 ggcgccgcct gcgcccgtga tgcgcgcctg ccgttgacac ctcaggcgat cggcactcct
     9121 atccgcgcat gggtattatc gtcggattca tcgcgctgat cctggcggtg ggcgcactgg
     9181 tgctggcctg gcctctggga tggctggtcg gtggctaccg ccgtgccgtg gtcctgctct
     9241 ccttgctgct gttcctcgcc ggatgggcca tcgtctgggt ccagccgatc gagcagagcc
     9301 gtcctcaaac ggccatttgg cccatcagcg tcggcggcag cggcgtgctc gcagtgatcc
     9361 tgatggcata cggccacgca ttcgcctggc tctggcggct ggtggcgcgc ctgctgggcc
     9421 gtggacagca aacctagggc acagcctgca tggtttgatt tccgctgttg cggccaagga
     9481 catccgcctc tagcttcttt cccgacacgc ccgccagagg cggacgcctg catcggtttc
     9541 tgtcaggagg agaacccatg tcgtcgctga aatcactcgc ctttgccgcg cttgcagcgg
     9601 cagctctcgc aagcgggccg gccctggccc agggcaagat caccgtcgcc cacaccgcga
     9661 gcggtgacta cgcctccggc ttctgggccg tcgacaaggg gctgttcaag aagcgcgggc
     9721 tcgatgtcga gttcaagctc gtgccactca atccccagat tccggcggcg gtgcaatccg
     9781 gctcggtgca ggtcggcgga ccgacgacga ccgtgctgtt gcaggcggtg gatggcggcc
     9841 tcgaccatgt ggcgctggcc ggctcggggc tgacgaggaa gaactccgac acattcggca
     9901 cgctcgcccg ggcggaggcg ccgatcagcg gcgccaaggg cttcgagggc aagaaggttg
     9961 gcgcgccggg catcggcgcc ttcctgcacg tgctgttctc gaagtggctg ctcgacaacg
    10021 gcgccgatct gaagaaggtc accttcgtcg aagtcgcctt cccgcagcac ctcgatgtgc
    10081 tcaagggcgg tacggtggac gccattgtca ccggtgagcc tttcacctcg cgcatcctgc
    10141 aagccggagt tggcaaggtt tcgacgcact tcaccgacaa cctgacgaac gacatgccga
    10201 tcatcgtcta ttccgcgacg cgtgcctggg cgcaggcgaa cccggccgcc gcgaaggcct
    10261 tcgccgaagc gatcgccgag gcgaccgagg cggtcaacaa cccggccaac gccgacgagg
    10321 tgcgcacggt gatcggcaag ttcatccaga tgccggaacc ggtgctgaag acactcaagc
    10381 tcggcacatg gaaggcggag gtcaccccgg ccggcctcaa ggactggatc gacacgatga
    10441 aatcgcaggg cctgatccag tccaatctcg acgtggcgaa aatcatctgg aagtgagatc
    10501 cgcacgacgc atcgtgcggc gcgccatccg tcctctgcca agagcggggg cgggtggccc
    10561 gaacacatcg gagggccgga tgaggggttg gtcccgcctt ccgggcaccg tacgcgtcgc
    10621 atgccgtcgg cggacgctcg acaggatgcc catgcactcc cattgccaga gttcgacgtg
    10681 accgcttcca tcgccttcga aaccacctcg atcgatctcg gcggacggac cattctcgat
    10741 gcgatcgact tcacggtcac gccgggaacc ttcgtgtcga tcgtcggcgc ctcgggcagc
    10801 ggcaagacca cgctgctgcg cctcgccgcc ggactttacc tgccgacgcg cggcagcgtc
    10861 cgcatcgacg gccgcgtcgt ctccggaccg gaccccgccg tcgccatcgt cttccaggac
    10921 tatggccggg ccctgctgcc ctggcgaacc gcggccggca acgtctccct cgcccttgag
    10981 gccatcggcc tgccgaaggc gcggcggccc gaccgc
//
LOCUS       DAOMXN010000063        10951 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_3211_length_10951_cov_12.448146, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000063 DAOMXN010000000
VERSION     DAOMXN010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 10951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10951
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3211_length_10951_cov_12.448146"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="PK812_06475"
     CDS             <1..483
                     /locus_tag="PK812_06475"
                     /inference="COORDINATES: protein motif:HMM:NF024592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4010 domain-containing protein"
                     /protein_id="HRJ69237.1"
                     /translation="AFADSTLDRLGPALVAGGAVYALGALTLARRGQDTEAGDAATRT
                     PFDFWAVLQMALLLAAIAFAARAASQHLGAGGVILVSALSGLADVDAAIVTAAGMIGG
                     AVKAPVAAKAMGAAIVTNTVAKAVYAAVLGGGRFALAFSGVSAAALAAGLALFVALSP
                     "
     gene            complement(488..1153)
                     /locus_tag="PK812_06480"
     CDS             complement(488..1153)
                     /locus_tag="PK812_06480"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HRJ69238.1"
                     /translation="MALFSARGFSTTYKPQVSGTEGDDTLYAIAGRGAVFAGGGNDWV
                     VGTSGNDQLYGGTGDDVLFGHGGNDRLYGEDGNDYIDGGTGQNVIDGGAGDDVINGSF
                     GHASMLYGGEGNDTIFGGHTGNGMSGGEGDDVLHLDGNLAAPNVGPFVFASGGEGSDT
                     LVFDGAIDVSSLQAFTVDGSFETLFVRWTGSNGSFHSGTVNSIEWIDAGNGVESILNY
                     VVA"
     gene            complement(1261..3714)
                     /locus_tag="PK812_06485"
     CDS             complement(1261..3714)
                     /locus_tag="PK812_06485"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HRJ69239.1"
                     /translation="MRRLGSILKWLGMTLAIIFVAAGVGAGWYTFAVLPPYTGELRLA
                     GLSGPVDVVRDKEGVPHIYAGSELDAVFAQGAVHAQDRLWQMEMHRRIANGRMAEVLG
                     PNALPIDRMLRTLGIRRTAEAMYRNLDPQSKAYFEAYARGVNAYLLQRTGPLPPEFYL
                     TSLPAIWNHISPEPWTPVDSIGWTLMMAWDLGGNWRTELQRLQLAQRGLTVEQINALL
                     PVSVNETWPSLASAQTLLTTLYKDMKAETGAVDRLFAALPGYEEGMGSNNWVVSGARS
                     ETGKPLLANDPHLGLIAPALWYFAHLSSPESDVIGASLPGVPGIILGRNKRIAWGFTN
                     TGPDVQDLFIEKLNPANPDEYQTPDGWKKFDIVEERIKIKGQADVVLKVRISRHGPIV
                     SDGGSDDHTRVAPKGHALAFAWAALHPENTTPAALAGIGRAKNWEEFLASAKLFQAPQ
                     QNMLYADVDGNIGYVAPGRVPIRKPENDLNGLFPAPGWDARYDWDGFIPFEELPREFN
                     PARGYRATANERIVDADYKPHITYEWATDHRARRIADVLPQKPKHTLASFRDLHADVV
                     SYAIREALPHLLKVTPAGDREKRAHDLLAKFDGTMLPDRAEPLIATAWLREVARMTYG
                     DEVGADLMKSFFDQRHLFVMSVISGQPGRDAWCDDMTSPAKETCPDIVAKALTAALAD
                     LEKRYGADWSKWRWGEAHAARSEHRPFSAVPALAKYFDVKVPTGGDTYTVNVGRHTVR
                     KEAEPFVSTHAASLRALYDLSDLEKSQFIHSTGQSGILFSPQYANLARRWADVQYIPM
                     VTDRKAAEKDALGTLKLKP"
     gene            3817..6576
                     /gene="ppc"
                     /locus_tag="PK812_06490"
     CDS             3817..6576
                     /gene="ppc"
                     /locus_tag="PK812_06490"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003610617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HRJ69240.1"
                     /translation="MRMADVAPAASDTEAPLIADIRLLGQILGDTVREQEGEAVFALI
                     ETIRRLSVAISREASREAADQLDALLRGLSPAEAVSVVRAFTYFSHLANLAEDRHHIR
                     RRVAHDEAGDRPQAGSLERCFDRLAAGGVTPEATKAMLDRAWVAPVLTAHPTEVQRKS
                     ILDAERAIAGLLAMRDGLRTPRERADNLKAMQARVAQLWQTRLLRTSKLSVSDEIENA
                     LSYYRATFLREIPRLYGRLEELLGQGRVAAFLRMGSWIGGDRDGNPNVTAETLRIALT
                     RQCETAIRHYLTEVHELGGELSVSQMLAGATPELLALADASGDSNSHRSDEPYRRALV
                     GVYARLAATLKDRTGEEALRHAVAPSTPYGSITEFLADLAIVDASLRAHHGAAMADLR
                     LAPLVRTAEVFGFQLATIDLRQNSDRHEAAVAELMKVARVAEDYSALPEARKLAVLTA
                     ALRDPRALRVPHRVYSAETESELDIFATARRLRSRIGRQAVRHAIISHTETVSDLLEV
                     LVLQKECGLMDGTLGESGARAALTVVPLFETIGDLRQAEAVMRGFFAIAGIAELVRSQ
                     NNEQEVMLGYSDSNKDGGYFTSNWELYRASTALARLFSQELGVTLRLFHGRGGTVGRG
                     GGPSYQAILAQPPGTVTGQIRLTEQGEVIASKYANPEIGRRNLETLAVAAIEATLLQS
                     AETAPADFLDAAEALSEASTQAYRALVYDDPDFADFFFAATPIAEIAELNIGSRPASR
                     KANRRIEDLRAIPWSFSWGQARVALPGWYGFGAAVEAFVAARGPSGETLLRRMYRDWP
                     FFHTLLSNMDMVLAKGDLAMLPRYAALAADGAQAKRISARIEEEWRRAERALAAITGA
                     TQRLAENPALARSIRHRFPYIAPLNHLQVELIRRWRAGDHDEKIRRGILISINGIAAG
                     LRNTG"
     gene            complement(6583..8091)
                     /locus_tag="PK812_06495"
     CDS             complement(6583..8091)
                     /locus_tag="PK812_06495"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRJ69241.1"
                     /translation="MNDLHIQLKRQIARATAPNGVLDIVRLTQMINEAYLEADKERRR
                     TDHSIRQMVSELDHRAQHDVLTNLANRSYFSSWLRQAIGSVTDLARIAVFFLDLDRFK
                     EVNDTLGHPAGDELLRSVAARLSEAVGEKGLVARLGGDEFAVALPLDAGTVTPAELAS
                     RIIHWMSEPFLIEGQLTSIGVSIGICLFPDNGSGPTDLLRCADMALYRAKHAGKGTFC
                     FFEDEMSRTARYQKAMEEGLREALERGSFELYFQPIVEAATMRNRTLETLLRWHHPTR
                     GLITPSDFIPVAETTGLIVPIGEWVIRNACLQARAFDPAVSVAINLSPAQLGSENIVR
                     VFEDALSESGVAASRIEIEITETVLMREDIQTRNVLQRLRQIGLRFSLDDFGTGYSSL
                     GYLQKFHFDKIKIDRSFCASVNSNPVNAALVRAIAGLGRDLGIAVVAEGIETAQQSAA
                     LAAEGCQYLQGYFHGRPAPAMDFVSNLDGAASMATRLRDTLRPPARELAAAS"
     gene            complement(8088..9248)
                     /locus_tag="PK812_06500"
     CDS             complement(8088..9248)
                     /locus_tag="PK812_06500"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HRJ69242.1"
                     /translation="MQAIEFTWSTGQGFSTPVAKAHDSDLVLYFGAREALEQGDWFAR
                     WREAFPRATIFGCSTGGQIMHADIVDGVIAAVALRFDRTRLRKVVAPIGLASGSRDCG
                     RNIGQQLVAHDLAGVLLLSDGLQVNGTHLVEGIVEVLGGQVPVCGGLAGDGAAFSTTL
                     VGLDAQPTSGLVAAVGLYGTDIAVGSGSAGGWEVFGPNRVVTRSEGNILRELDGQPAL
                     DLYERYLCADDIAGLPGTALLYPLLIRNPECSKQELVRTILAVDRQARTMTFAGDMPV
                     GWSAQLMRGSFERLAEGAASAAKSAADAIVQIGKDQPPTSVAVMISCIGRRLMMGQRV
                     SDEIEAAMACFPPGTAGIGFYSYGEIAPHAATGFCDLHNQTMTIMTLSERAA"
     gene            9644..>10951
                     /gene="dnaG"
                     /locus_tag="PK812_06505"
     CDS             9644..>10951
                     /gene="dnaG"
                     /locus_tag="PK812_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HRJ69243.1"
                     /translation="MRYPPSLLDDIRARLPVSAVVGRRVKLTRAGREWKGLSPFNQEK
                     TPSFFVNDQKGSFFDFSSGKNGDIFKFVMETEGLSFPEAVERLAAEAGVELPKVSVEA
                     ERFEQQRKGLGEAMELAAQWFQQQLKERAGAGAREYLKGRDLPPAVQAEFRLGYAPNE
                     RFGLRDFLAGKGVPVEVMVEAGLLISVEEVAVPYDRFRDRVMFPISDARGKVIAFGGR
                     ALQKDAQAKYLNSPETPLFHKGATLYNLNKARKPAHEKGNLIAVEGYVDAIAMTRAGF
                     PETVAPLGTALTEDQLALMWRIAGEPVLCFDGDKAGVKAAHRAIDVALPHLQPGRTLR
                     FAFLPGGQDPDELLRSSGPDAIAAVVNRPRSFVDVLFAREAEREPLDTPERRADLEKR
                     LREAVSAIKDETVRKHYREAMDERIRALFRQSGPQRGGSRGGPD"
BASE COUNT         1829 a         3530 c         3725 g         1867 t
ORIGIN      
        1 gcattcgcgg actcgaccct ggaccgcctc ggcccggcgc tggtcgcggg cggagcggtc
       61 tatgcccttg gcgctctgac gctggcccgc cggggacagg acacggaggc tggcgatgcc
      121 gcgacccgca cgccattcga cttctgggcc gtgctgcaga tggctttgct gctcgccgcc
      181 atcgccttcg ccgcccgggc ggcgtcccag cacctgggcg ctggcggcgt catcctggtt
      241 tcggcgctct ccggcctcgc cgatgtggat gcggccatcg tcacggccgc cggcatgatt
      301 ggcggagcgg tcaaggcgcc agtcgccgct aaggcgatgg gggcggcgat cgtcaccaac
      361 accgtcgcca aggcggtcta tgcggcagtg ctcggcggcg gccgcttcgc gctggctttc
      421 tccggcgtct ccgccgccgc gctggcggcg ggcctcgcgc tgtttgtcgc gctgtcgccg
      481 tgagacgtca ggcgacgacg tagttcagga tcgattccac gccgttgccg gcgtcgatcc
      541 attcgatgga attcacggtg ccgctgtgga atgagccgtt cgaaccggtc cagcggacga
      601 acagggtttc gaaagatcca tccacggtga aggcctgaag gctcgacacg tcgatggcac
      661 catcgaagac gagcgtgtcg ctgccctcgc cgccggaggc gaagacgaag ggaccaacgt
      721 tcggtgcggc gagattgcca tcgaggtgca ggacatcgtc gccttcgccg cccgacatgc
      781 cattgccggt gtggccgccg aagatcgtat cgttgccctc gccgccgtag agcattgagg
      841 cgtgtccgaa cgagccgttg atgacgtcat cgccggcgcc gccatcgatg acattctggc
      901 cggtgccgcc atcgatgtag tcgttgccgt cctcaccgta gagccggtcg ttgccgccat
      961 ggccaaacag gacgtcgtcg ccggttccgc cgtagagctg gtcgttgcca ctcgttccca
     1021 ccacccagtc attgccaccg ccggcgaaaa ccgccccgcg tccggcgatg gcgtagagcg
     1081 tgtcgtcgcc ctccgtgccg gaaacctggg gcttgtaggt ggtggaaaag ccgcgcgcgg
     1141 aaaacagagc catgatgcac cttgctgttg caacccggcg aatgccgccg gtttcagcaa
     1201 ggattgctca aggctgtccg tccgcctgtg cgctggcgca cggcgcgtgt atcggcgatc
     1261 tcacggcttc agtttcaggg tccccaacgc gtccttttcc gccgccttcc ggtcggtgac
     1321 catggggatg tactgaacat ccgcccagcg cctggccaga ttcgcgtatt gcggcgagaa
     1381 caaaatgccg gactggccgg tggagtggat gaactgcgac ttctcgaggt ccgagaggtc
     1441 gtagagcgcg cgcagggacg cggcatgcgt gctgacgaag ggttcggcct ccttgcgcac
     1501 ggtgtggcgg ccgacgttga cggtgtaggt gtcgccgccg gtcggcacct tgacgtcgaa
     1561 gtacttggcc agcgccggaa ccgcactgaa cggccggtgt tccgatcgcg cggcatgcgc
     1621 ctcgccccag cgccacttgg accagtccgc tccgtagcgc ttttcgagat cggccagggc
     1681 ggcggtcagc gccttcgcga cgatgtccgg gcaggtctcc ttcgccggcg aggtcatgtc
     1741 gtcgcaccag gcgtcgcgac cgggctggcc ggaaatgacc gacatgacga acagatgccg
     1801 ctggtcgaag aagctcttca tcaggtccgc gcccacttca tcgccatagg tcatgcgggc
     1861 gacctcgcgc agccacgccg tggcgatcag cggctcggcg cggtcgggca gcatggtgcc
     1921 gtcgaacttc gccaggaggt catgggcgcg cttctccctg tcgcccgccg gcgtgacctt
     1981 gaggagatgc ggcagagcct ccctgatggc gtaggaaacg acatcggcat gcaggtcgcg
     2041 gaagctggcc aaggtatgct tgggcttctg cggcagcaca tcggcgatgc ggcgggcgcg
     2101 atggtccgtc gcccattcat aggtgatgtg cggcttgtaa tcggcgtcca cgatgcgctc
     2161 gttcgccgtg gcgcgatagc cgcgtgccgg attgaactcg cgcggcagct cctcgaacgg
     2221 gatgaatccg tcccagtcgt agcgtgcgtc ccagccgggc gccgggaaca ggccgttgag
     2281 gtcgttctcc ggcttgcgga tcggcacgcg gccgggcgcg acatagccga tgtttccgtc
     2341 cacgtcggcg taaagcatgt tctgctgcgg ggcctggaac agcttcgccg aggcgaggaa
     2401 ctcctcccag ttcttcgccc ggccgatgcc ggcgagcgcc gccggggtgg tgttttccgg
     2461 gtgcagcgcc gcccaggcga aggcgagcgc gtggcccttc ggcgcgaccc gggtgtggtc
     2521 gtcgcttccg ccgtcgctga cgatcggccc atggcgggag atgcgcacct tcagcaccac
     2581 gtcggcctgc cccttgatct tgatccgctc ctcgacgatg tcgaacttct tccagccgtc
     2641 cggcgtctgg tactcgtcag gattggcggg attgagcttc tcgatgaaga gatcctgcac
     2701 gtccgggccg gtgttggtga agccccaggc gatgcgcttg ttgcggccga ggatgatgcc
     2761 cggcacgccc ggcagcgacg cgccgatcac gtcggattcc ggcgaggaca gatgcgcgaa
     2821 ataccacagc gccggggcga tgaggcccag gtgcgggtcg ttggcgagca ggggcttgcc
     2881 cgtctctgaa cgcgcgccgg agacgaccca gttgttggac cccatgcctt cctcatagcc
     2941 gggcagggcg gcgaagagcc ggtcgacagc ccccgtttcg gccttcatgt ccttgtagag
     3001 cgtggtcagc agggtctgcg ccgaggcgag cgagggccat gtctcgttga cagagaccgg
     3061 cagcagggcg ttgatctgct ccacggtgag gccgcgctgg gcgagttgca ggcgctggag
     3121 ttcggtgcgc cagttgccgc cgaggtccca ggccatcatc agcgtccagc cgatggaatc
     3181 gaccggcgtc cagggttcgg gcgatatgtg gttccagatg gccggcaggc tcgtgagata
     3241 gaactccggc ggcaaaggac cggtgcgctg caacagatag gcgttcacac cgcgggcata
     3301 ggcttcgaaa taggccttcg attgcggatc gaggttgcgg tacatcgcct ccgccgtccg
     3361 gcggatgccg agggtgcgca gcatgcgatc gatgggcagg gcgtttggcc caaggacttc
     3421 cgccatgcgg ccgttggcga tgcgccggtg catttccatc tgccagagcc ggtcctgcgc
     3481 gtgcacggcg ccctgcgcga agaccgcgtc cagctcgctg ccggcataga tatgcggcac
     3541 gccctccttg tcgcgcacga catcgaccgg gccgctgagg ccggcgaggc gcaattcccc
     3601 ggtataggga ggcagcacgg cgaacgtgta ccagccggcg ccgacgccgg ccgcgacgaa
     3661 gatgatcgcc agcgtcatgc caagccattt cagaatgctt ccgagccgcc gcatgcgctt
     3721 ctcccgttcc tggccgggga gcgtctgttt cgggcacccg gcgaattgca actttggtct
     3781 ctatcctgca tggagcggga cgcagggcaa ggaggcatgc gcatggcaga tgtcgcaccc
     3841 gccgcttcgg ataccgaggc accgctgatc gccgacatcc ggctgctcgg ccagattctc
     3901 ggcgacaccg tgcgcgagca ggagggcgag gcggtcttcg ccctgatcga gaccatccgc
     3961 cggttgtcgg tggcgatcag ccgcgaggca tcgcgcgagg cggcggacca gctcgatgcg
     4021 ctgctgcggg gcctgagccc ggcggaggcc gtgagcgtgg tgcgcgcctt cacctacttc
     4081 tcgcatctgg cgaacctggc cgaggaccgt caccacatcc gccgccgtgt cgcccacgac
     4141 gaagccggag accggccgca ggcgggatcg ctggaacgct gtttcgaccg gctcgcggcc
     4201 ggaggcgtca cgccggaagc gacgaaggcg atgctcgacc gcgcctgggt ggcgccggtg
     4261 ctcacggctc atcccaccga ggtgcagcgc aagagcatcc tcgatgccga acgcgccatc
     4321 gccgggctgc tggcgatgcg tgacggattg cgcaccccgc gcgagcgggc ggacaatctc
     4381 aaggcgatgc aggcgcgcgt cgcgcagctg tggcagacgc gcctgctgcg cacctcgaag
     4441 ctgtccgtct cggacgagat cgagaacgcg ctgagctatt atcgcgccac gttcctgcgc
     4501 gagataccaa gactttacgg ccggctggag gagctgctgg gacagggacg tgttgccgcc
     4561 ttcctgcgca tgggcagctg gatcggcggg gaccgggacg gcaaccccaa cgtgacggcg
     4621 gagacgctca ggatcgctct cacgcgccag tgcgagacgg cgatccgtca ctacctgacc
     4681 gaggtgcacg aactgggcgg cgaactttcg gtctcgcaga tgctggcggg cgccacgccg
     4741 gaactcctgg cgctcgccga tgcctcgggc gacagcaaca gtcatcgctc cgacgagccc
     4801 taccgacgtg ctctcgtggg cgtctatgcc cggcttgcgg cgacgctgaa ggatcgcacc
     4861 ggcgaggagg cgctgcgcca tgccgtcgcg ccctccacgc cctatgggtc gatcacggaa
     4921 ttcctggccg acctcgccat cgtcgatgcc tcactgcggg cgcatcacgg tgcggcgatg
     4981 gctgacctga ggcttgcgcc gctggtgcgc accgcggaag tgttcggttt ccagctcgcg
     5041 accatcgatc tcaggcagaa ctccgaccgg cacgaggcgg cggtggcgga actgatgaag
     5101 gtcgcgcgcg tcgcggaaga ctacagtgcc ctgcccgaag cccggaagct cgccgtgttg
     5161 accgcggcgt tgcgcgatcc gcgcgcgctc cgcgtgccgc accgggtcta ttccgccgag
     5221 accgaaagcg aactggacat cttcgcgacc gcccgcaggc tgcgttcgcg cattggccgg
     5281 caggcggtgc gccacgccat catcagccat accgaaacgg tcagcgacct gttggaagtg
     5341 ctggtgctgc aaaaggaatg cggcctgatg gacggcacgc tgggagagtc aggcgcgcgc
     5401 gcggccctca ccgtggttcc cctgttcgag accatcggcg atctcaggca ggcagaggcg
     5461 gtgatgcgcg gcttcttcgc cattgcgggc atcgcggagc tcgtgcgcag ccagaacaac
     5521 gagcaggaag tcatgctcgg ctattccgat tccaacaagg acggcggcta tttcacctcg
     5581 aactgggaac tctaccgcgc ctcgacggcg ctggcccggc tgttctcgca ggagctcggc
     5641 gtcacgctca ggctgttcca tggccggggc ggcacggtgg gacgcggcgg cgggccgagc
     5701 tatcaggcga tcctcgccca gccgcccggc acggtgacgg gccagatccg gctgaccgaa
     5761 cagggggagg tcatcgcctc gaaatacgcc aatccggaga ttggccggcg caacctcgag
     5821 acgcttgcgg tcgcggcgat cgaggcgacg ctgctgcaat cggccgaaac cgctccggcg
     5881 gatttcctcg atgcggctga agccctttca gaggcctcca cgcaggccta ccgcgcgctg
     5941 gtctatgacg atccggattt cgccgatttc ttcttcgccg cgacaccgat cgccgagatc
     6001 gccgaactca acatcgggtc gcggccggcg tcccgcaagg ccaaccggcg catcgaggat
     6061 ctgcgcgcca ttccctggag cttttcctgg gggcaggcgc gcgtggcgct gcccggctgg
     6121 tacgggttcg gcgcggcggt cgaggctttc gtcgcggccc gggggccatc gggcgagacg
     6181 ctgctccggc gcatgtatcg cgactggccg ttcttccaca cgctgctctc gaacatggac
     6241 atggtgctgg ccaagggcga tctcgccatg ctgccgcgct atgccgcgct ggcggccgac
     6301 ggggcgcagg cgaaacgcat ctccgccagg atcgaagagg aatggcggcg cgcggaacgg
     6361 gcgctcgcgg cgatcaccgg cgcgacgcag cggctagccg aaaacccggc gctggcacgc
     6421 tccatccgcc accgctttcc ctatatcgcg ccgctcaacc atttgcaggt ggaactgatc
     6481 cgccgttggc gcgccggcga ccacgatgag aaaatccggc gcggcatcct catttccatc
     6541 aacggcatcg cggcggggtt gcgcaatacg gggtgagggc ggttacgagg ctgcggcgag
     6601 ttcgcgcgcg ggcgggcgca acgtgtcacg caggcgcgtg gccatgcttg cggcaccatc
     6661 gaggttcgag acaaagtcca tagccggcgc cggccggccg tggaaatatc cctgcaggta
     6721 ttggcagccc tcggcggcca gtgcggcgct ctgctgcgcc gtttcgatcc cctccgccac
     6781 gactgcaatg ccaaggtcac gaccgagacc ggcaatagcg cggaccagcg cggcattgac
     6841 cgggttggaa ttgacgctgg cgcagaagga ccggtcgatc ttgatcttgt cgaagtggaa
     6901 tttctgcagg tagccgagtg aggaatagcc ggtgccgaag tcgtcgagcg agaagcgcaa
     6961 gccgatctgg cgcagccgct gcaagacatt gcgggtctgg atgtcctcac gcataaggac
     7021 ggtctcggtg atctcgatct cgatgcgcga tgccgcgaca ccgctctcgc tcaaggcgtc
     7081 ctcgaacacg cggactatgt tctccgatcc caactgggct ggcgacaggt tgatggccac
     7141 cgaaactgcc ggatcaaacg ctcgggcctg caggcaggca ttgcggatga cccattcgcc
     7201 gatcggaacg atgaggccgg tcgtctcggc gaccgggatg aaatccgacg gcgtgatcag
     7261 gccacgagtg gggtggtgcc agcgcaatag cgtctccagg gtgcggttgc gcatggtcgc
     7321 agcttcgacg atgggctgga aatagagctc gaaactgccc cgctccagcg cctcgcggag
     7381 gccttcctcc atcgcctttt ggtaccgcgc tgtgcgcgac atctcatcct cgaaaaagca
     7441 aaaggtcccc ttgccggcat gcttggcgcg gtacagcgcc atgtcggcgc atcgcagcag
     7501 atccgtgggg ccgctgccgt tgtccgggaa aaggcagatg ccgatgctga cgccgatcga
     7561 ggttagctgc ccctcgataa ggaacggctc cgacatccag tggatgatgc gactggccag
     7621 ttccgcgggc gtgaccgtgc cggcgtcgag cggcagcgcc accgcaaatt cgtcgcctcc
     7681 gagccgcgcc acgaggcctt tttccccgac ggcctcggac agccgcgcgg caacggagcg
     7741 caggagctca tcgccggctg gatggccaag cgtgtcgttg acctccttga aacgatcaag
     7801 gtcaagaaaa aagacggcaa tgcgcgccag atcggtgacc gagccgatcg cttgcctgag
     7861 ccatgacgag aaataactcc gattggcaag gttggtcagc acgtcgtgct gggcgcgatg
     7921 gtcaagttca gaaaccatct ggcgaatgga atggtccgtg cggcggcgct ccttgtccgc
     7981 ttcgagatag gcctcgttga tcatctgcgt cagtcgaacg atatcgagta caccgttcgg
     8041 agcggttgcg cgtgcgatct gccgtttcag ctgtatgtgc agatcgttca tgccgcgcgc
     8101 tccgaaaggg tcatgatggt catggtctgg ttgtgcaagt cgcagaaccc tgtcgcggca
     8161 tgcggcgcga tctctccgta ggaatagaag ccgatgccgg cggttccggg cgggaaacag
     8221 gccatggccg cctcgatctc atcggagacg cgctggccca tcatcagacg acgaccgatg
     8281 caactgatca tcacggccac ggaggttggt ggctgatcct tgccgatctg cacgatggcg
     8341 tcagcggcgg atttcgctgc gcttgccgcg ccttcggcca gacgctcgaa ggagccgcgc
     8401 atcagttgcg ccgaccagcc gaccggcatg tcgccggcga aggtcatggt gcgagcctgg
     8461 cgatccaccg ccagaatggt gcgcaccagt tcttgcttgc tgcattccgg gttgcggatc
     8521 agcagaggat agagcaacgc cgtgccgggc aggccggcga tgtcatcggc gcagagatag
     8581 cgctcgtaca agtcgagggc cggctgacca tcaagctcgc gcaggatatt gccttccgag
     8641 cgggtgacaa cgcggttcgg cccgaaaact tcccagccac cggcgctgcc agagccgaca
     8701 gcgatgtccg tgccataaag acccacagcg gcgacaagac cacttgtggg ttgcgcgtcc
     8761 agacccacga gcgtagtcga aaatgccgca ccgtcgcccg cgaggccgcc gcaaactggg
     8821 acctggcctc ctaacacttc gacgatgccc tcgaccagat gcgtgccgtt gacttgcagc
     8881 ccatcggata gcagcagcac gccggccagg tcatgcgcca ccaactgctg gccaatgttt
     8941 cgcccgcagt cccgagagcc cgaggcaagg ccgataggcg caaccacctt ccgcaaccgc
     9001 gtcctgtcga agcgcagggc gacagccgcg atgacgccgt cgacgatgtc ggcgtgcatg
     9061 atctggccgc cagtgctgca gccgaagatt gtcgcccggg ggaatgcctc gcgccagcgg
     9121 gcaaaccagt cgccctgttc cagcgcctcg cgcgcgccga aataaaggac caggtccgaa
     9181 tcgtgcgctt tcgcgaccgg tgtgctgaaa ccctgacccg ttgaccatgt gaactcgata
     9241 gcctgcatgg attcagcccc tcatagtcgc agacctgcaa tacaattaca ggttgtatct
     9301 tacaggggag acgtttaacg aaatcttccc gcaattttct acgcacgggc aggaaatgcg
     9361 ccatgacgcc cggcatttcg ggcaaaagac gctccacagt ttcaggcatg caaatgcggc
     9421 cggcggcatt tgtgtaagag tttagcagtt ggatgggcaa ggaaaccagc cgctgcaacc
     9481 cagggtagcg cgtcggtcgt gacgcatttt gccccgtccg tttcattccg gctgtggatt
     9541 gcggggagaa aacggcggct ggcgatctgt cttctcgaag cgctggatgc gcgctgcgct
     9601 tcttctgccg cctccgggaa gatgtgctat gggagaaatc cccatgcgct accccccttc
     9661 gctcctcgac gacatccgcg cccggctgcc ggtttcggcc gtcgtcgggc ggcgcgtgaa
     9721 gctgacccgc gccgggcgcg agtggaaggg gctttccccg ttcaaccagg agaagacgcc
     9781 ctcgttcttc gtgaacgacc agaagggctc atttttcgat ttttcctccg gcaagaacgg
     9841 cgacatcttc aagttcgtga tggaaacgga ggggctgtcg tttcccgagg ccgtggagcg
     9901 gctggcggcg gaagccggcg tcgaactgcc gaaggtttcg gtcgaggcgg agcggttcga
     9961 acagcagcgc aagggcctcg gcgaggcgat ggaactcgcg gcgcagtggt tccagcagca
    10021 gctgaaggag cgggccgggg ccggcgcgcg ggagtatctc aaggggcgcg acctgccgcc
    10081 cgccgtgcag gcggaattcc gccttggcta tgcgccgaac gagcgcttcg ggctgcgcga
    10141 tttcctcgcc ggcaagggcg tgcccgtcga agtcatggtg gaagcgggtc tgttgatctc
    10201 ggtcgaggag gtcgcggttc cctatgaccg gttccgcgac cgggtgatgt tcccgatcag
    10261 cgatgcgcgc ggcaaggtca tcgccttcgg cgggcgggcg ctccagaagg atgcacaggc
    10321 aaaatatctc aattcgccgg aaaccccgct cttccacaag ggcgccacgc tctacaacct
    10381 caacaaggcg cgcaagcccg cgcatgagaa gggcaatctc atcgcggtgg agggctatgt
    10441 cgacgccatc gccatgaccc gcgccggctt tccggaaacg gtggcgcctc tcggaacggc
    10501 gctgaccgag gaccagctgg cgctgatgtg gcgcatcgcc ggcgagccgg tgctgtgctt
    10561 cgatggcgac aaggcggggg tgaaggcggc gcaccgcgcc attgacgtgg cgttgccgca
    10621 tcttcagccc gggcgcacgc tgcggttcgc cttcctgcca ggcgggcagg accccgatga
    10681 gctgctgcgc tcgtccgggc cggatgccat cgccgccgtg gtcaacaggc cgcgcagttt
    10741 cgtcgacgtg ctgttcgccc gcgaggcgga gcgcgagccg ctggatacgc ccgaacggcg
    10801 cgccgacctt gaaaagcggc tgcgcgaggc ggtttccgcc atcaaggacg agacggtcag
    10861 gaagcattac cgcgaggcga tggacgagcg tatccgggcg ctgttccgtc agtccggtcc
    10921 gcagcggggc ggcagtcgcg gcggaccgga c
//
LOCUS       DAOMXN010000064        10896 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_3227_length_10896_cov_11.523199, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000064 DAOMXN010000000
VERSION     DAOMXN010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 10896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10896
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3227_length_10896_cov_11.523199"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="PK812_06510"
     CDS             complement(<1..888)
                     /locus_tag="PK812_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HRJ69244.1"
                     /translation="MAVLTLADAQDLVSVALQRNRTEAGNAASVARALVAAEADGQKG
                     HGLSRVPSYAAQAKAGKVDGYAHPTISRARAGLLVVDAAHGFAYPALDTAMAVLPSIA
                     AANGVAAAAVHRSHHCGAAGLVVESLADKGMVALLFANTPAAMAPWGGSVGVFGTNPI
                     AFACPLPEAPALVIDLSLSKVARGNLVAAKQRGETIPEGWAVDNDGKPTTDPDAGLAG
                     TMVPMGDAKGTALALMVELLAAGLTGANFAADASSFLDAKGPPPGTGQLLIALDCGAV
                     GGPAALARFARLAASIEGQP"
     gene            complement(891..1817)
                     /locus_tag="PK812_06515"
     CDS             complement(891..1817)
                     /locus_tag="PK812_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009451164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HRJ69245.1"
                     /translation="MADVVITEFMDEAAVADLRRTHDVHYDANLVDKPAELLRLAAGA
                     RALIVRNRTQVRGELLASARTLICVGRLGVGLDNIDLEACRARNIAVLPATGANDLGV
                     AEYVVGTAMVLLRGAYASTGEVVAGKWPRNRLMGREIAGKTLGLIGFGSIGRLTAQLA
                     RALGMSVAAFDPHLPADDPRWAEAERLELDALLTSADVISLHVPLTDATRRLLDASRL
                     ARMKSGAILINAARGGVIDEAALAAALKAGRLGGAALDVFEEEPLSAARGAVFEDCPN
                     LILTPHIAGVSVESNTRVSWLTVENVRRTLGG"
     gene            complement(1834..2985)
                     /locus_tag="PK812_06520"
     CDS             complement(1834..2985)
                     /locus_tag="PK812_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009467086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HRJ69246.1"
                     /translation="MGWRRENGRMGVRNHVVILPLDDLSNAAAEAVANNIKGTLAIPH
                     AYGRLQFGADLDLFFRTLIGTGSNPNVAAVVVIGIEEGWTKTVVDGIAATGKPVVGFG
                     IEGHGDISTIARASYVAKEFVQWASELPREKASITELWVSTKCGESDTTTGLSSCPTV
                     GNMYDKLIPRGIYGVFGETSEITGAEHLCRARAIDKKTGDRWYKMWKSYQDDVIEAHK
                     TNDLSDSQPTKGNIAGGLTTIEEKALGNLEKIGRECKYIDILEPAEAPAKGAGLYFMD
                     TSSAAAECVTLMAAAGYVVHTFPTGQGNVIGNPIVPVIKISGNPKTLRTMPEHIDVDV
                     TGVLKRTMTIPEAGDALIECIVRTSNGRFTANEALGHREFSLTKLYRSA"
     gene            complement(3160..3447)
                     /locus_tag="PK812_06525"
     CDS             complement(3160..3447)
                     /locus_tag="PK812_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HRJ69247.1"
                     /translation="MANPHLLVHEHKDTVGVVVVEGLRAGADMLCVRTCDNSDFHLKA
                     KMDIPIGHKVALVDIKKGDTIWKYGQDIGKAIADIKKGEHLHVHNVKTKRW"
     gene            3608..4363
                     /locus_tag="PK812_06530"
     CDS             3608..4363
                     /locus_tag="PK812_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015930271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRJ69248.1"
                     /translation="MENTTPPPAGGSGTNEPLGFRPLYRQVRDRLMRRIADGYWPPGQ
                     MLPSEAQIAGEFGVSQGTVRKALDEMAAEHILVRRQGRGTFVSEHNEQRVLFQYFKLV
                     PDDGEREFPESVALSCDQDQASAQERAMLDLPAGAGVIRIRRMRSLRGKPCVSEHITL
                     PAVLFTGLAESEIPNNLYALYAARYGVTIARSTEKLKAVGAPPGDADVLRVARGTPVL
                     RVDRLARSIEGSAAEWRIAYCLTDSFHYLSDLR"
     gene            complement(4342..5217)
                     /locus_tag="PK812_06535"
     CDS             complement(4342..5217)
                     /locus_tag="PK812_06535"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRJ69249.1"
                     /translation="MPTAFIALLVYPVMALQFAVTKIGVAAGLTAVDIGGLRYLGALL
                     LGFPLFLRADVRSMMRHRFGPFLAVGLLGGGFYGMVFATAIALTPASHGAVIVPSLSI
                     TVAMVGTGLLTGAWPDRARWVGLALIIGGLLVFAAGSKTGSSGFSALGDALLIVIGLM
                     WGSASLAMKYWQVPPAAVAASFTLTGIGAVGYWLGMEGMAAAIAAPGASLLQMVFQGG
                     LLTVLAFALWGELVRRIGPTRTALGVASVPPLALVTAVPLLGEWPTTLQYLGTGVVVA
                     GIALASGAAQRRSDR"
     gene            complement(5235..6029)
                     /locus_tag="PK812_06540"
     CDS             complement(5235..6029)
                     /locus_tag="PK812_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HRJ69250.1"
                     /translation="MARVEVTITDHVAEVALARPEKMNALDPAMFEAIAAAGEGLKSN
                     PDVRAVVLHGRGPSFCAGLDFQSFMTMGADAATIEARLFTLASGEVANMFQKPSHVWQ
                     ELEVPVIAALNGVAYGGGCQIALGADIRIAGPDARLSVMEIKWGLVPDMGITQSIRRL
                     VRVDVAKELVLTGRVVGAEEARDLGLVTRIAADPLAAARELAREIAGKSPDATRRIKK
                     LLDTAWYAAPAEGLRLEARLQHEVIGKPNQIEAVRANFEKRAPNFR"
     gene            6187..6690
                     /locus_tag="PK812_06545"
     CDS             6187..6690
                     /locus_tag="PK812_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HRJ69251.1"
                     /translation="MMDERVRALAHELLESGVEHLSERERKIIARIAHRHHISRNVSA
                     DYDDRRTLGEIWADRLALWGGSWLFVLAFALFLLCWGGANAGVLPQAWRFDPYPFVFL
                     NLVLSMLAAIQAPVILMSQNRQAAKDRLQAMQDYEVNLKAELEIMALHDKLDRLAETV
                     QAAVLRR"
     gene            complement(6687..7826)
                     /locus_tag="PK812_06550"
     CDS             complement(6687..7826)
                     /locus_tag="PK812_06550"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRJ69252.1"
                     /translation="MTGYSPAVGPYDPPLIATKALGLTLAAVFAAELALSFGPLGTML
                     APSLQTLVAFGGASRGLVVGDGEWFRLLTMAFAHAHLLHLAMNGLGLWLVGRTLEPVL
                     GARWFLAVFFVGALSGSISSVSFNPPGIVGVGASGALMGLFGFLLAFALRLPSGEHRK
                     AFATSALSVLIPSLAPALLPLFLPGQGLQIDYAAHAGGALAGAAIAALSPLFWPKIWI
                     PVGYRQIAGAIVAAGILVCGYGAVQLASRHDEYKLLARLIPAAEIPATEAAFQEQSAD
                     LVARFPEDPRARFHRAIALIRAGDAQGAEREARTAMEIAERYPRSFTRDFRDWARLLL
                     AGAQQEQGRMAEARTTARPLCATSAGTGRIRDLLNRYKLCEQERP"
     gene            complement(7831..8769)
                     /gene="mepA"
                     /locus_tag="PK812_06555"
     CDS             complement(7831..8769)
                     /gene="mepA"
                     /locus_tag="PK812_06555"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HRJ69253.1"
                     /translation="MRAIALAVMAMLPCLLLPARAQQTMSEEMKTRARLMEIMPADAA
                     KRHFGGAKTAAAGDVRTIGSYAKGCLAGATALALDGPTWQVMRTSRNRNWGHPELIAL
                     VERLAKRGPKEANWPGLLVGDISQPRGGPMLTGHASHQVGLDADIWLTPMPPRTLNED
                     ERENMAATNAVAADWMDVDPKAWTPAHRDIVRLAALEPRVTRIFVNPAIKKAMCRDPG
                     RDREWLTKVRPMWGHNYHFHIRIACPAGDAACIDQDPPPEGDGCGKELSDWLALQHKT
                     IFGPKKPKDPNAKPKPPVFMPLSAMPEACQQIVLRR"
     gene            9134..9556
                     /locus_tag="PK812_06560"
     CDS             9134..9556
                     /locus_tag="PK812_06560"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007067111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRJ69254.1"
                     /translation="MNRIIAFFILAFGLIAGTASAEAKVVARINIAQQKMVVSVDGEV
                     QHVWSVSTARKGYVTPRGTYAPKRMHVKYYSKKYYNSPMPYSIFFKGGYAVHGTTAVG
                     KLGAPASHGCIRLATGNAAKLYQLVRQHGPGKARIVIT"
     gene            9702..10145
                     /locus_tag="PK812_06565"
     CDS             9702..10145
                     /locus_tag="PK812_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69255.1"
                     /translation="MASGAQQDLVRKLVFGFLAGALAVLVFHQATIFMLGVLKVIPAG
                     NAWSFRPNPWRVPVLVNSMFWGGLWGIAYTFVQNRLPRSRWLGGFVFGVLGPALVGSW
                     LVVALLKGNPVFAGFAPPRLLIGALINGVFGIGVAVFHDLIGRRL"
     gene            complement(10240..>10896)
                     /locus_tag="PK812_06570"
     CDS             complement(10240..>10896)
                     /locus_tag="PK812_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HRJ69256.1"
                     /translation="LADADACRSIGLDRRQALWEARRLPQSAPLPLFAAAQAGELGAE
                     PEAMLPAMSLGEHVATDYQTLRLSLRAHPMALLRSFFALENVVTCAETSRRADGRFTR
                     TAGLVLVRQRPGKGNAIFITLEDETGITNCVLWARQFEALRRPVMAARLMLVEGRVQR
                     SPEGVVHLMATHIVDRSAEIARLWDAGQETARPERPPLRARHRHPRDVRVLPRSRDFH
                     "
BASE COUNT         1873 a         3524 c         3637 g         1862 t
ORIGIN      
        1 tggttggccc tcgatggaag ccgcgagccg cgcaaagcgt gccagcgcgg ccggtccgcc
       61 cactgcgcca caatccagcg cgataagcaa ttgtccggtg ccgggcggcg gtcccttcgc
      121 atcgaggaac gaagaagcgt ccgccgcgaa attcgcgccg gtcagccccg cggcgagcag
      181 ttcgaccatc aacgccagcg ccgtcccctt ggcgtcgccc atcgggacca tggttccagc
      241 cagtcccgcg tcgggatcgg tagtcggctt gccgtcattg tcgactgccc agccttccgg
      301 aattgtctcg ccccgctgct tggcagcgac aaggttgccg cgcgccacct tcgacagcga
      361 caggtcgatc accagcgccg gagcctccgg cagcgggcag gcaaacgcga tcggattggt
      421 gccgaaaacc ccgacggatc caccccaggg ggccatggcg gccggtgtgt tggcgaacag
      481 cagcgcaacc atgcctttgt ccgccaggga ttcgaccacg agccccgccg ctccgcaatg
      541 gtgcgagcga tggacggccg ccgccgccac gccattggca gccgcaatcg acggcagcac
      601 ggccatggcc gtatcgagtg ccggataggc aaagccgtgc gccgcatcga cgaccagcag
      661 tccggctctg gcccggctga tggtgggatg agcatatcca tccaccttcc cggccttcgc
      721 ctgcgcggca taacttggca cgcgggagag gccgtggccc ttctggccgt ccgcttcggc
      781 cgccacaagt gcccgggcca cgctggcggc attgccggcc tccgtgcggt tgcgctggag
      841 cgcgaccgag accaggtcct gagcgtcagc cagggtcagt acagccacgg tcagcccccc
      901 agggtccggc ggacattctc gaccgtcagc caggaaacac gcgtgttgga ttcgacagag
      961 acgccggcga tgtgcggcgt cagtatcagg ttcggacagt cctcgaacac ggcaccgcgc
     1021 gccgcagaca ggggctcctc ctcgaaaacg tccagcgctg ctccaccgag tcggcccgcc
     1081 ttcagtgctg cggccagcgc cgcttcgtcg atcacgccgc cacgggccgc gttgatcaga
     1141 atggcaccgg acttcatgcg ggcaaggcgc gaggcgtcaa gcagcctgcg cgtcgcgtcg
     1201 gtgagaggca catgcagcga gatgacgtca gccgaagtca ggagcgcatc gagctccagg
     1261 cgctcggcct cggcccagcg cggatcatcg gccggaagat gcggatcgaa ggcggccacg
     1321 gacatgccga gtgccctggc gagctgcgcg gtcagccgcc cgatcgagcc gaagcctatg
     1381 agcccaaggg tctttcccgc gatctcccgc cccatgagcc ggttgcgcgg ccattttccg
     1441 gccaccacct cgcccgtcga ggcataggcg ccccgaagga gcaccatggc cgtgccaacg
     1501 acatattcgg caacgccgag atcgtttgcc cccgtcgcgg gaagaaccgc gatgttgcgc
     1561 gcacggcagg cctcaaggtc gatgttgtcg agaccgacgc caagccgacc cacgcagatg
     1621 agagtgcgcg ccgacgccaa gagctcgccg cgcacctggg tccggttgcg gacgatcagc
     1681 gcgcgcgcgc cagccgccag ccgcagcagt tccgccggct tgtccacgag gttggcatcg
     1741 taatgcacgt catgcgtgcg ccggaggtcg gcgacggcgg cctcatccat gaattcggtg
     1801 atgacgacat cggccatggc gcgtgccgtg acctcaggcc gagcgataca gcttggtcag
     1861 cgagaactcg cgatgaccca gtgcctcgtt ggcggtgaaa cgtccgttcg acgtgcgtac
     1921 gatgcattca atcagcgcat cgcccgcctc cggaatggtc atggtccgct tcaggacacc
     1981 ggtcacgtcc acgtcgatgt gctcgggcat ggtgcgcagg gtcttggggt tgccggagat
     2041 cttgatcacc ggaacgatcg gattaccgat gacgttgccc tgtccggtcg ggaaggtgtg
     2101 cacgacatag ccggcggcag ccatcagcgt cacgcattcc gccgcggccg acgatgtgtc
     2161 catgaagtac agccccgccc ctttcgccgg cgcctcggcc ggttcgagga tgtcgatgta
     2221 cttgcactcg cggccgatct tctcgaggtt gccgagcgcc ttctcctcaa tggtcgtgag
     2281 gccgccggcg atgttgccct tggttggctg gctgtccgac aggtcgttgg tcttgtgcgc
     2341 ctcgatcacg tcatcctgat aggatttcca catcttgtac cagcggtcgc cggtcttctt
     2401 gtctatggcg cgggcacgac acaggtgttc ggcgccagtg atctccgaag tctcgccgaa
     2461 cacgccgtag atcccgcgcg gaatcagctt gtcgtacatg ttgccgacag tggggcagga
     2521 ggacaggccc gtggtcgtgt cggattcgcc gcacttcgtc gacacccaaa gctccgtgat
     2581 cgaggctttc tcgcgcggca gttcgctcgc ccattggacg aattccttcg cgacataaga
     2641 ggctctggcg atggtcgaga tgtcgccatg gccctcaatg ccgaagccga cgaccggctt
     2701 gccggtcgct gcgatgccgt cgaccacggt cttggtccag ccttcctcga tcccgatcac
     2761 tacgacggca gcgacgttcg ggttcgagcc cgtgccgatc agggttcgga agaataggtc
     2821 gaggtcggcg ccaaactgca gtcggccgta ggcatgggga atcgccagcg tgcccttgat
     2881 gttgttggcc acggcttcgg cggccgcgtt ggacagatcg tcaagcggca ggatcaccac
     2941 atggttgcgc acgcccatgc ggccgttctc gcgccgccag cccatgaagg agtccagcgc
     3001 gcgaccactg ggccgcttgc ctgcgggcgc cttgaaggcg ggtgcctcga tcttgcggcc
     3061 cttgaaaccc ttcagcttgc gattgacgag gtcgaaattg gcgacgatgc ccatggcgaa
     3121 aatcctgtga accgggagaa atcggacgag tggcggcgct taccagcgct tggtcttgac
     3181 gttgtggacg tgcagatgct cgcccttctt gatgtcggcg atcgccttgc cgatgtcctg
     3241 gccgtacttc cagatcgtgt cgcccttctt gatatcaaca agcgcgacct tgtggccgat
     3301 cggaatgtcc atctttgcct tcaggtggaa gtcggaattg tcacaggtgc gcacgcacag
     3361 catgtccgcc cctgccctga gcccttcaac cacgaccacg ccgacggtgt ccttgtgttc
     3421 gtgcaccaac agatgcggat tcgccatgag aaagcctcgc tgatggaaag ggagcttagt
     3481 cttatataag acataggacg cggcactgtc aacgcaactc cgggagcgag ttcgactgag
     3541 gcgctggaac gccggtcctg tatctgttag aagacctaac tcttgatggt tcccggcgga
     3601 gacgctcatg gagaacacca cccctcctcc ggcaggcggg tccggcacca acgagccgct
     3661 cggcttccgc ccgctatatc gtcaggtgcg cgatcggttg atgcggcgca tcgccgacgg
     3721 ctactggcct ccaggccaga tgctgccaag cgaggcacag attgccgggg agttcggcgt
     3781 gtcgcagggc accgtgcgca aggcgctcga cgagatggcg gcggagcata tcctggtgcg
     3841 tcgccagggg cgcggcacgt ttgtgtcgga gcataacgag caacgggtgc tgttccagta
     3901 tttcaagctg gttcccgacg atggcgaacg cgaattcccc gaaagcgttg ccctgtcctg
     3961 cgaccaggac caggcgagtg cccaggagcg tgcaatgttg gatctgccgg ccggagcggg
     4021 cgtgatcagg atccggcgca tgcggtcctt gcgcggcaag ccctgtgtct cggaacacat
     4081 caccctgccg gcggtgctgt tcacggggct ggcggagtca gagatcccca acaacctcta
     4141 cgcgctctat gccgcacgct acggcgtaac cattgcccgg tcgacggaaa aacttaaggc
     4201 ggtcggcgcg ccgccgggcg atgccgatgt gctgcgcgtc gcgcgcggta ccccggttct
     4261 cagggtggac cggctggcgc gctcgatcga gggcagcgcg gcggagtggc gcattgccta
     4321 ctgcctgacc gattccttcc actacctgtc agatctgcgt tgagccgctc cgctcgcgag
     4381 cgcgattccc gcgacgacga caccggtgcc cagatactgg agcgtcgtcg gccattcacc
     4441 cagcaaaggg acggccgtga ccagcgccag cggtggaacg ctcgccacgc cgagagcggt
     4501 gcgcgtggga ccgatgcggc gcaccagttc accccagagt gcgaaggcga ggaccgtcag
     4561 cagcccgccc tggaacacca tctgcagcag cgaggcgccc ggcgccgcga tggcggccgc
     4621 catgccttcc atgcccagcc agtagcccac ggcgccgatg ccggtcagcg tgaaactggc
     4681 ggcgaccgca gccggtggta cctgccagta cttcatggcg agggacgccg agccccacat
     4741 caggccgatc acgatgagca gggcgtcgcc cagcgccgag aatccgcttg aacccgtctt
     4801 ggagccggcc gcgaaaacca agaggccgcc aatgatcagg gcaaggccga cccagcgcgc
     4861 ccggtcaggc catgcgcccg tgagcagtcc ggtgccgacc atggccacgg tgatcgacag
     4921 ggagggcacg atgacggcgc cgtgggaagc cggcgtcagt gcgatggctg tggcgaacac
     4981 catgccatag aaccctccgc cgagcagacc gacggcgagg aacggaccga aacggtgccg
     5041 catcatggag cgcacgtcgg cgcgcaggaa cagcggaaaa ccgagcagca gcgcgccgag
     5101 atagcgcagg ccgccgatgt cgaccgccgt gagccctgcc gccacgccga tcttggtgac
     5161 ggcgaattgc agcgccatga cgggatagac cagcagggcg atgaacgcgg tcggcatggc
     5221 gacacgaccg gcggtcagcg gaaattcggc gcgcgtttct cgaagttggc gcgaaccgcc
     5281 tcgatctggt tcggcttgcc gatcacctcg tgctgcaaac gggcttccag gcgcaggcct
     5341 tcggccgggg cggcatacca ggcggtgtcg agcagcttct tgatgcggcg cgtcgcatcc
     5401 ggcgactttc cggcgatctc gcgtgccagt tcgcgcgcgg cggccagcgg atcagccgcg
     5461 atgcgggtga caagcccgag gtcccgagcc tcctcggcgc caacgacgcg gccggtcagc
     5521 accagttcct ttgcgacgtc gacgcggacc agccgtcgga tcgactgggt gatccccatg
     5581 tctggcacga ggccccattt gatctccatg accgagagcc gtgcatccgg cccggcgatg
     5641 cggatatcgg cgcccagcgc gatctggcag ccgccgccgt aggcgacgcc gttcagcgcg
     5701 gcgataacgg gcacctcgag ttcctgccag acatgggacg gcttctggaa catgttggcg
     5761 acctcgccgg acgcgagggt gaacaggcgt gcctcgatcg tggcggcatc ggcgcccatg
     5821 gtcatgaagg actggaaatc gagacccgcg cagaaactcg gaccccggcc gtggagaacc
     5881 acggcccgca cgtccggatt ggacttcaga ccctctccgg cggcggcaat ggcttcgaac
     5941 attgcgggat cgagggcgtt cattttctcc gggcgcgcca gcgccacttc cgcgacatga
     6001 tcggtaatgg tgacttcgac gcgcgccatg gtgccttccc ttcgcagtgc cggatcgagt
     6061 gcgattcaac agcagggtag agcattgtcg ggcgcggggc accttccaag tgctttgccc
     6121 caggacaagg ccggatggaa acggcgcgct atcctggaac ccggcccaat acttccggag
     6181 ctggtcatga tggacgagag ggtgcgggcg ctggcccatg agctgctcga aagcggcgtg
     6241 gaacatctgt ccgagcgcga gaggaagatc attgcccgga tcgcccatcg ccatcacatc
     6301 tcgcgcaatg tcagcgcgga ctatgatgac cggcgcacgc tgggcgagat ctgggctgac
     6361 cggctggcct tgtggggcgg ctcctggctg tttgtcctcg ccttcgccct gttcctgttg
     6421 tgctggggtg gcgcgaacgc aggagtgctg ccccaggcgt ggcggttcga tccctacccg
     6481 ttcgtctttc tgaacctcgt cctgtcgatg ctcgcggcga tccaggcgcc ggtgatcctg
     6541 atgtcgcaga accgtcaggc agcgaaggac cggctgcagg cgatgcagga ctatgaagtg
     6601 aacctcaagg ccgaacttga aatcatggcg ctgcacgaca agttggatcg tctcgccgag
     6661 actgtgcagg ccgccgtctt gcgccgctag ggccgctcct gttcacaaag cttgtagcgg
     6721 ttcaggagat cgcggatgcg gccggtgccg gcagaggtcg cacagagcgg ccgcgccgtt
     6781 gtgcgcgcct cggccatccg cccctgctct tgctgcgccc cggcgagcag aaggcgtgcc
     6841 cagtcgcgga aatcccgggt gaagctgcgc ggatagcgct cggcgatttc catggcggtg
     6901 cgcgcctcgc gttcggcgcc ctgcgcatcg ccggcccgga tcaacgcgat ggcgcgatgg
     6961 aagcgcgccc gcgggtcttc ggggaagcga gcgacgagat cggctgactg ctcctggaac
     7021 gctgcttcgg tggctggaat ctcggcagcc gggatcaggc gcgccagcag cttgtattcg
     7081 tcatgccgtg aggcgagctg caccgcgcca tagccgcaga ccaggatgcc tgcggcgacg
     7141 atggcgccgg cgatctggcg ataccccacc ggaatccaga ttttcggcca gaacagcggc
     7201 gacagcgcgg cgatcgcggc acctgccagg gcaccgccgg catgggccgc atagtcgatc
     7261 tggagcccct gtcccggcag gaacagtgga agcaaggccg gagccaggga cggaatcaac
     7321 acgctcaacg cgctggtcgc gaatgccttg cgatgctcac cggacggcag gcgcagggca
     7381 aaggccagca ggaaaccgaa cagccccatc aatgcacccg aggcgccaac tcccacgatg
     7441 ccgggcggat tgaaggagac cgacgaaata ctgcccgaca gcgcgccgac aaagaaaacc
     7501 gcaaggaacc agcgtgcgcc gaggaccggc tcgagcgtcc gtccgaccag ccacagaccg
     7561 agcccattca tggccagatg gagcagatgg gcgtgcgcga acgccatggt cagcaggcga
     7621 aaccactcgc cgtcgcccac cacgaggcca cggctggctc cgccgaaagc aacaagggtc
     7681 tgcaggctgg gcgccagcat ggtgcccagc ggtccgaagc tgagcgcaag ctctgcggcg
     7741 aacaccgccg caagggtcag cccaagcgct ttggttgcga tcagcggggg atcatacgga
     7801 ccgacggcag gagaataccc tgtcaacgga tcatcgcctc agcacgattt gctggcaggc
     7861 ctcgggcatg gccgacaggg gcatgaacac cggtggcttt ggcttggcgt tgggatcctt
     7921 gggcttcttg ggtccgaaga tcgtcttgtg ctgcagcgcc aaccagtccg acagttcctt
     7981 tccgcagccg tcgccttccg gtggcgggtc ctggtcgata caggcggcat cgcccgctgg
     8041 gcaggcgatg cggatgtgga agtgatagtt gtgtccccac attgggcgca ctttggtcag
     8101 ccactcgcga tccctgccgg ggtcgcggca catcgccttt ttgatggcgg gattgacgaa
     8161 aatgcgcgtc acgcgcggtt ccagcgcggc gaggcgcacg atgtcacgat gggccggcgt
     8221 ccacgctttt gggtcgacgt ccatccagtc cgccgccacg gcgttggtgg ccgccatgtt
     8281 ctcgcgttcg tcctcattga gggtgcgtgg cggcatgggc gtgagccaga tgtctgcgtc
     8341 gagcccgacc tggtgcgagg cgtggcccgt cagcatcggt ccgccgcgcg gctgcgaaat
     8401 gtcgccgacc agcagcccgg gccagttcgc ttctttcggt ccccgcttcg ccagccgttc
     8461 cacgagcgcg atgagctcgg gatggcccca gttgcgattg cgcgaggtcc gcatgacctg
     8521 ccaggttggc ccgtcgagcg cgagcgcggt cgcgccggca aggcatccct tggcgtagga
     8581 gccgatggtc cgtacgtctc cggcggcggc ggtcttcgcg ccgccgaaat gacgcttggc
     8641 cgcgtcggcc ggcatgatct ccatcagccg cgcccgcgtc ttcatctctt cggacatcgt
     8701 ctgctgggcc cgagcgggaa ggagcagaca gggcagcatc gccatgacgg ccagagctat
     8761 cgcgcgcatc cgatcctcgt tcagtaagat tcgagtcgtt accttaacac gctggaatca
     8821 tttcataaac cggcagatta ccgtgatctc tgggaaacga aacgtcaacc accggaattc
     8881 actccaacga aaggggtgtg gcggttttct gtccgcacag ggaacgacag gtggcgcgcc
     8941 ggaagagcgg gattcgactc cggacgcgca atctgcctga ctgatgcgga gggccgcgac
     9001 cggcggcgcc ccgatcaatt cgggtatcgc tcgccatgca gctgagcatg ggagagactt
     9061 ggcacgatcc agatggcggc aaggtccttg ccctgcgatc tggcaaacag aggcgtaacg
     9121 acagggtggc gtcatgaaca ggatcattgc gttcttcatt ctggccttcg gcctgatcgc
     9181 cggaaccgcc agcgcagagg ccaaggtggt ggcccgcatc aatatcgcgc agcagaaaat
     9241 ggtggtcagc gttgacggcg aagtgcagca tgtctggagc gtgtcgactg cgcgcaaggg
     9301 ctatgtcacg ccgcgcggca cctatgcccc caagcgcatg cacgtgaagt actattccaa
     9361 gaagtactac aactcgccca tgccttattc gatcttcttc aagggcggct atgcggtgca
     9421 tggcacgaca gccgtcggca agctcggcgc tcccgcatcg cacgggtgca tccggctggc
     9481 gaccggcaat gcggcaaagc tctaccagct cgtccgtcag catggtccgg ggaaagcgcg
     9541 catcgtgatc acgtgacggc ctggccttct tctgacgttc acggcaatgt gatggccgct
     9601 cttgtgcggg gccgccagcc caccgggcgc gtgtctgaat agggggtcga ctcttcggtg
     9661 cgcctcggct tcatttccct cggtccgagg gggaaacgac aatggcttca ggggctcaac
     9721 aggatctggt gaggaaactc gtcttcgggt ttctggctgg agcgctggca gtgctggtgt
     9781 tccatcaggc gacgattttc atgcttggtg tcctgaaggt gatacccgcc ggcaatgcct
     9841 ggtccttccg gccgaatccc tggcgcgttc cggtcctggt caattccatg ttctggggcg
     9901 gtctttgggg cattgcctac acgtttgtgc agaacagact gccaaggtcg cgctggctgg
     9961 gcgggttcgt gttcggggtg cttggtccgg ctctggtcgg ctcctggctg gtggtggcgc
    10021 ttctgaaggg caatccggtc ttcgccggtt tcgcgccgcc tcgcctgctc atcggagcgc
    10081 tgatcaacgg ggtgtttggc atcggcgttg cggtgtttca cgatctgatc gggagaaggc
    10141 tgtagttttc cggcctttgc gaaaggcgaa caggacggga aatctgcggg gttctatggg
    10201 ccgatgcagg ccgccgggcc ccggcgcatg tcggccacgt caatggaaat cccggctgcg
    10261 cggcaagacc cggacgtcgc gcggatgacg gtggcgcgcc cggagcggcg gtcgttccgg
    10321 ccgcgctgtc tcctgtccgg catcccagag ccgcgcgatc tcggccgaac gatcgacgat
    10381 atgtgtggcc atcaggtgca cgacgccctc cgggctgcgc tgcacgcggc cctcgacgag
    10441 catcagccgc gccgccatca ccggccggcg caaggcctcg aactggcgcg cccagagcac
    10501 gcagttggtg atgccggtct cgtcctccag cgtgatgaag atcgcgttgc ccttgccggg
    10561 ccgctggcgc accagcacca gcccggccgt gcgtgtgaag cggccgtcgg cccgcctcga
    10621 ggtctcggcg caggtcacga cattctcaag cgcaaagaag gaccgtagca gtgccatcgg
    10681 gtgcgccctt agggacaggc gtaaggtctg gtagtccgtc gcgacatgtt cgccgagact
    10741 catggccggg agcatcgctt ccggctccgc gccgagttcg cccgcctggg cggcggcgaa
    10801 caggggcagg ggggccgatt gcggcaggcg ccgggcttcc caaagtgcct gccgccggtc
    10861 gaggccgatg gagcggcagg cgtcggcatc ggccag
//
LOCUS       DAOMXN010000065        10894 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_3228_length_10894_cov_10.461758, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000065 DAOMXN010000000
VERSION     DAOMXN010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 10894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10894
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3228_length_10894_cov_10.461758"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="PK812_06575"
     CDS             complement(<1..443)
                     /locus_tag="PK812_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRJ69257.1"
                     /translation="MMRLLCLGYGYCAQAFVARYRALFTGIAATVRRQEQVAAVESAG
                     IAAVALTEQDDGGLASAISSSAVLLVSAGPDATGDPFVARVASALGSSGSGRTILYLS
                     TVGVYGDHAGGWVSEETPPAPGSARSRYRLAAEHAWLGLAASSGA"
     gene            complement(440..1567)
                     /gene="queG"
                     /locus_tag="PK812_06580"
     CDS             complement(440..1567)
                     /gene="queG"
                     /locus_tag="PK812_06580"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015951009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HRJ69258.1"
                     /translation="MTGSDLIGAVRARAAALGFEAFGVAPAEAAPQTLGRLRQWLAEG
                     LHGDMLWMAEAPERRAHPTELWPQVRSILVLGANYAGETDPLAALSARDKASIALYAR
                     RKDYHDVLKGRLKELAGFLAARGGGDVKVFIDTAPVLEKTLAAQAGLGWQGKNTMLVS
                     RRHGNWLFLGAIYTTLALPDERPEPDRCGSCRRCLVACPTEAFPRPYVLDARRCISYL
                     TIEHRGVIPQRLRGAIGNRVFGCDDCLAVCPWNKFARAAHDQKLAQRVELEAPDLRHL
                     VRLDETAFRLYFAGTPVKRTGRDRFVRNVLIAVGNSTDPTLLPEVEALLDDPSPLVRG
                     MAVWALGRLADADRFAAERDRRLIAETNGEVRAEWWQPEEE"
     gene            complement(1564..2256)
                     /locus_tag="PK812_06585"
     CDS             complement(1564..2256)
                     /locus_tag="PK812_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRJ69259.1"
                     /translation="MPALFHHVLCPHSRYVRLIMGELGMEATLSEERVWERRREFLAL
                     NPAGNTPVLLEENVFAIPGADVIAEYLDETRGLVLGDRRLLPEAPAPRIEVRRLTDWF
                     NRKFYEEVSHYLVAEKVHKRFLPMDAGGGAPEGAILRAARANVRYHLKYVGHLLGQRN
                     WLAGDTLSYADLAAAAHFSVVDYLGDVPWSEDEMAKQWYARVKSRPSFRPILTDRLLG
                     MNPSQHYADLDF"
     gene            2421..3224
                     /locus_tag="PK812_06590"
     CDS             2421..3224
                     /locus_tag="PK812_06590"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HRJ69260.1"
                     /translation="MTLISMLQAALLGFIEGLTEFLPVSSTGHLLLVGHFLKFDSPNK
                     TFEVLVQFGAILAILSVYFGKVTSLALGMFTDPARRRFVIAVLIAFVPAMILGGLFSK
                     VIKSYLFNPFLVCTSLIAGGLVLLVVDETKFKTTYNDMMEYPLPMALKIGAFQCLAMI
                     PGVSRSGATIVGAMLLGSSKRAAAEFSFFLAMPTMAGAFAKDLLDNYKVLAAKDFGLI
                     FIGFVMAFISGLIVVRGFLDFVSRHGFRPFAYWRIAVGTLGLIGLMVLG"
     gene            complement(3221..3976)
                     /gene="nth"
                     /locus_tag="PK812_06595"
     CDS             complement(3221..3976)
                     /gene="nth"
                     /locus_tag="PK812_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HRJ69261.1"
                     /translation="MMTDSKMQKKSPRASGRASAGAGRRQDAATLTDCEVRDVFERLH
                     ALNPEPKGELEYVNAYTLLVAVVLSAQATDAGVNKATRKLFALADTPEKMLALGEDGV
                     RDLIKTIGLFRNKAKNVILLSRRLIDAFAGAVPADREALVSLPGVGRKTANVVLNIAF
                     GQPTIAVDTHLFRVSNRLPIARGTTPEQVEHGLERRVPDAFRLHCHHWLILHGRYICK
                     AQKPECWRCPLAEICRFEPKSPPPRPKSPSYTA"
     gene            4059..4517
                     /locus_tag="PK812_06600"
     CDS             4059..4517
                     /locus_tag="PK812_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HRJ69262.1"
                     /translation="MPVFAATLRPNRPLGRSGVALVVGFVALIATVTAIPFIVAGAWP
                     IGGFFGLDVALLYGAFRISSRRAQAYEEVVLSRLELCLRKVDWRGRAREWRFNPFWAR
                     LRTREQAEFGLTDLAIVEHGREVAVGSFLGPQEKQEFARALDCALADARR"
     gene            4637..5197
                     /locus_tag="PK812_06605"
     CDS             4637..5197
                     /locus_tag="PK812_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRJ69263.1"
                     /translation="MTGWILLAIIVLPILWAIMIYNGLVAMRQRANQSFADIDVQLKQ
                     RHDLIPNLVETVKGYATHEKDTLDAVIKARAAAVSAHGPQAQGQAEQALTGALGRLIA
                     ISEAYPDLKANANFQQLQGELSDIENKLAAARRFFNNSVSEFNAGIEQFPAVLFASKF
                     GFLPMAFFDVGEEQRKTLDVTPQVKF"
     gene            5212..6294
                     /locus_tag="PK812_06610"
     CDS             5212..6294
                     /locus_tag="PK812_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRJ69264.1"
                     /translation="MAGAFGLYSHIQANRRRSFLLIAGLFALIYALVFAGALAAAGLR
                     GGPSLNWMLARAFDDLITHAPLATGLTLIWIVIAYLFHQKMIDAITDSHSVERKDEPK
                     LYNQLENLCISRGVPMPQLKIMESEVLNAFASGLNEKQYCVTVTRGLMQTLNDEEMEA
                     VLAHELTHIRNGDVKLMVTAVIIAGVVSFFAELLFRWLARGPTIRFSDDNGDSKKASP
                     AAAALMIAVVIVVVAWVASGLLRFALSRTREYLADAGAAELTKNPDALISALLKISGR
                     AEIPGAPSGLMEMCIENERSGFVDVFATHPPIEDRVEALVKFAGGHMPAPEAGPKPSF
                     LPETVQLDSKLGPWGQAAAAANPWRR"
     gene            complement(6313..7485)
                     /locus_tag="PK812_06615"
     CDS             complement(6313..7485)
                     /locus_tag="PK812_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007199158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HRJ69265.1"
                     /translation="MSSIDPRIAALKDEVASWRRDIHAHPEILYEVHRTAALVAEKLK
                     SFGVDEVVTGIGKTGVVGVIKGRKTASGKVVALRADMDALPMSEQTNLPYASKVANMM
                     HACGHDGHTAMLLGAAKHLAADRGFDGTVVVVFQPAEEGGAGGKAMVDDGLVSRFGIQ
                     EFYGLHCVPGVPVGHFMTRPGPLMAATANFVITIEGKGAHGAQPHQGIDPVLIGSHMV
                     TALQSIASRNTDPLKSVVVSVTTFNAGDAFNVIPQTAVLRGTVRFLEKAVGQVTEERF
                     RAIVANTAKAFGAEARLDFTYGYPATVNHQAETEFAVAAAATVAGAGGVIANAPPVMG
                     GEDFSYMLEAKPGAFLFIGNGDTAPLHNPAFDFNDEAIPAGISYWTAVAQRAMPLR"
     gene            complement(7562..8815)
                     /locus_tag="PK812_06620"
     CDS             complement(7562..8815)
                     /locus_tag="PK812_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69266.1"
                     /translation="MEHKTERFPESPAADAAGLLLRLGFGLLVMVAPVSALFSRRAFI
                     VLVPVGALLIVAAALLRDSGGLSHRLRDAVLSPTGMAALGLGIWCALSLAWTPFPGPA
                     AERLLRTLGIGVLALAVVSALPQRMRASNLHLMSIGVAAATLALIVAAVLGPSALRFL
                     RDLETPTIGRAAAAAGVMVWPAVAWTMTRGRDGQAFSLIAVCGVAIAASGSLDAAVVM
                     ILALAVFLAARTMPTTTGRMLSIAFPAAVLAAPILAFAARFLARAMRLRADHDLTEIG
                     HWADAIAAQPIRLITGHGFDTALRARLAGLVDPRAPGGLISDTWYDLGLIGATGLALV
                     LWLMFRTLAGLAQPVAATALAVATAIAAFAIIDPTALQVWWLCVMLVAALMLAAVESG
                     QYRTTRPAALVQQGGPRRPVARIGR"
     gene            9002..9577
                     /locus_tag="PK812_06625"
     CDS             9002..9577
                     /locus_tag="PK812_06625"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HRJ69267.1"
                     /translation="MTNGEPLYASLLARRSAKAKDLIAPGPDSGQIERMVLAASRAPD
                     HGRLVPFRFVMIGEDRRTAFATVLAAAAKQENPGLSASEVDRARDKAFEGPCLIAMIA
                     RVDVAHPKIPASDQWLTAGCALENLLLAAQALGFAVAVRSGRYLEMAVVREAFALAPV
                     EHFVCFLAVGTPADYPPAKPKPSLDQVLSRW"
     gene            complement(9579..>10894)
                     /gene="phnD"
                     /locus_tag="PK812_06630"
     CDS             complement(9579..>10894)
                     /gene="phnD"
                     /locus_tag="PK812_06630"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_function: GO:0043225 - ATPase-coupled inorganic anion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HRJ69268.1"
                     /translation="AAPRPAIEARGDARPATELRTPTPEMRTQMLGSPPPFLPNLRDQ
                     LPPERRVNPSEIRVQTPVVVENRYVDYRIPDRAPFDFRPLDYKPVDTRTTDYKSQDNK
                     TPDQKVLDPRLKEKGPEIQARIDCRAILFRAVECNFLDYREPDPKLLELSAKYPEINL
                     GIVPKEFSKEQIDRWSPLITYLSREIGLKISLKVANDYQALLESQKAGLIHMAIYSPL
                     FFAKARLAGSKIEAFAVETNADGGRGAHSVVYAMARGPNPKVEDLKGRSIGLVDPNSL
                     TGYSVARFALASQKLDPDNFLGKQVFTGSHENALVALSQGLVDLAVGEWKSDEDSTLT
                     RLLARGVLKNVDGSPMRRDDFRIVLKSEPLVNSPIAYLADMPEDLKAMLRRAMLEAPM
                     RDRSAFERIYDTKGKTWEIIDTKAYDGTVDLLRYMEESRKRNKQS"
BASE COUNT         1883 a         3585 c         3556 g         1870 t
ORIGIN      
        1 cttgcgccgc tcgaagccgc gaggccaagc caggcgtgct cggccgccag acggtagcgg
       61 ctgcgcgcgg agcccggcgc gggcggcgtt tcctccgaca cccagccgcc ggcgtgatcg
      121 ccatagacac cgacggttga aagataaagg atcgtcctgc cggagccgga cgagccgagc
      181 gcggatgcga cgcgcgccac gaagggatca cccgtcgcgt caggtccggc ggacacgagc
      241 aggacggcgc tcgagctgat cgcgctggcg aggccgccgt cgtcctgttc cgtcagggca
      301 acggcggcga tcccggccga ttccactgcg gcgacctgtt cctgacgccg caccgtggcg
      361 gcgataccag taaacaacgc ccgataccgc gcgacgaaag cctgtgcgca gtagccgtac
      421 cccaggcaaa gcaggcgcat cattcctcct ccggttgcca ccattcggca cggacctccc
      481 cattggtctc ggcgatcagg cgcctatcac gttccgccgc gaaccggtca gcatccgcca
      541 gccgccccag cgcccaaacc gccatgccgc gcaccagggg cgatggatcg tccagaagcg
      601 cctcgacctc cggcaacagc gtcggatcgg tcgaattgcc gacggcgatc agcacattgc
      661 gcacgaaccg gtcgcgcccc gtgcgcttga ccggggttcc ggcgaagtac aggcggaagg
      721 cggtttcatc gaggcgcacc aagtgccgga gatccggcgc ctcgagctcg acgcgctgcg
      781 ccagcttctg gtcgtgggcg gcccgggcaa acttgttcca gggacagacg gccaggcaat
      841 cgtcacagcc gaacacccgg ttgccgatgg ccccgcgcag ccgctgcgga ataacgcccc
      901 tgtgctcgat ggtcagatag gaaatgcaac ggcgcgcatc cagcacgtag gggcgcggaa
      961 aagcctcggt cggacaggcg accaggcacc ggcgacagga gccgcagcga tccggttcgg
     1021 gcctttcatc aggcagcgcc agcgtggtgt agatcgcgcc gaggaacagc caattgccgt
     1081 gacggcgcga gaccagcatc gtgttcttgc cctgccagcc gaggccggcc tgcgccgcca
     1141 gtgtcttttc cagaaccggc gcggtgtcga tgaacacctt gacgtcgccg cctccgcgcg
     1201 ccgccaggaa gccggccagt tccttcagcc tgcccttgag gacgtcgtgg tagtccttgc
     1261 ggcgggcgta gagcgcgatc gacgccttgt cccgcgccga aagagccgcc agcgggtccg
     1321 tttcccccgc gtagttcgcg ccgaggacca ggatgctccg gacctgcggc caaagctcgg
     1381 tcggatgcgc acggcgctcc ggcgcctcgg ccatccagag catgtcgccg tggagccctt
     1441 ccgccagcca ctgccgcagc cgtcctaggg tctggggggc ggcttcagcc ggcgcgacgc
     1501 caaaggcctc gaacccgagc gctgcggcac gcgcacgaac ggctccgatc aggtcggagc
     1561 cggtcagaag tcgagatccg cgtagtgctg gctcgggttc atgccgagca gccgatcggt
     1621 caggatcgga cggaatgacg gccgcgactt gacccgcgcg taccattgct ttgccatctc
     1681 gtcctcggac caaggcacat ccccgagata gtccacgacc gaaaaatgcg cggcagccgc
     1741 cagatcggca taggagagcg tgtcgcctgc cagccagttc cgctgcccca gcagatgccc
     1801 gacatacttc agatgatagc gcacattggc gcgcgccgcg cgcaggatcg cgccttccgg
     1861 cgcgccaccg cccgcatcca tgggcaggaa gcgcttgtgc accttttccg caaccaggta
     1921 gtgggacact tcctcataga acttgcggtt gaaccaatcg gtcaggcggc gcacttcgat
     1981 gcgcggcgcg ggcgcctcgg gcaacagacg tcggtcgcca agaaccagcc cccgcgtttc
     2041 gtccagatat tccgcgatta catccgcacc tggaatggcg aagacgttct cctccagcag
     2101 gaccggggtg ttgcccgcgg gattcagcgc gagaaactcg cggcggcgct cccacacgcg
     2161 ctcctcggag agcgtggcct ccatgccaag ctcgcccatg atcaggcgga catatcgtga
     2221 atgcggacag agaacgtgat ggaacagcgc tggcatgaac gtgcaaagca acggcgcagg
     2281 cgagagattc gcctgcttct gaccttggtg ggtcgcctgc gaagcgtata gaagcaaaat
     2341 ggattcgcct caatcattgt ccagtcgggc ggaccgatga gctgcgacac gttccgcgca
     2401 gggcgcgcaa tcgaggggcc atgacactta tctccatgct tcaggctgcc ttgctcggct
     2461 tcatcgaggg cctgaccgag ttcctgccgg tttcgtccac cgggcacctg ctgctggtcg
     2521 gtcatttcct gaaattcgat tcgccgaaca agaccttcga ggtgctggtg caattcggcg
     2581 ccatcctggc gattctttct gtctacttcg gcaaggtgac gagcctcgcg ctcggcatgt
     2641 tcaccgatcc ggcgcgccgc cggttcgtga tcgctgtgct gatcgcgttc gtgccggcaa
     2701 tgatcctcgg cggccttttt tcgaaagtga tcaagagcta tctcttcaat ccgttcctgg
     2761 tctgcaccag cctgatcgcc ggcggactgg tgctgctcgt cgtcgatgag accaagttca
     2821 agacgacgta caacgacatg atggagtatc cgctgccgat ggcgctgaag atcggcgcgt
     2881 tccagtgcct ggcgatgatt cccggcgtgt cgcggtccgg cgcgaccatt gtcggtgcga
     2941 tgctgctggg gtcctccaag cgcgcggcgg cggaattttc ctttttcctc gccatgccga
     3001 ccatggcggg cgccttcgcc aaggaccttc tggacaatta caaggttctc gccgccaagg
     3061 atttcggcct gatcttcatt ggcttcgtga tggcgttcat ttccgggctg atcgtggtgc
     3121 gcggctttct cgatttcgtc tcgcgccacg gatttcgtcc cttcgcttac tggcgcatcg
     3181 cggtcgggac gctagggctg atcggcctca tggtgctcgg ctaagccgta tacgacggcg
     3241 atttcggccg gggcggcggc gatttcggct cgaaccggca gatttccgcc agaggacaac
     3301 gccagcactc gggcttctgc gccttgcaga tgtagcggcc gtgcagaatc agccagtgat
     3361 ggcaatgcag ccggaaggcg tcgggcacgc gcctttcgag tccgtgctca acctgttccg
     3421 gcgtggtccc gcgcgcgatc ggcagccggt tcgagacgcg gaacagatgc gtgtccacgg
     3481 cgatcgttgg ctgtccgaag gcgatgttga gcaccacgtt cgccgtcttg cgaccaacgc
     3541 ccggcaggct caccagcgcc tcacgatcgg ccggcacggc gccggcgaaa gcatcgatca
     3601 gacgcctcga gagcaagatg acgttcttcg ccttgttgcg gaacaggccg atggtcttga
     3661 tcaggtccct gaccccgtcc tcaccgagcg ccagcatttt ctcaggcgta tcagccaggg
     3721 cgaacagctt gcgcgtcgcc ttgttgacgc cggcgtccgt cgcctgcgcc gacagcacga
     3781 cagcgaccag cagcgtgtag gcattgacgt attccagttc gcccttcggc tcgggattga
     3841 gcgcgtgcaa gcgttcgaac acatcgcgaa cctcgcaatc tgtcagcgtc gccgcatcct
     3901 ggcgcctgcc cgcgccggcg ctcgccctac ccgacgcgcg cggggacttt ttttgcatct
     3961 tcgagtctgt catcatgcgc gcaccgtcgg aagccatcgg ggggattgtg ccgaacgctt
     4021 ctttcgatac cgggcgaggc gtcttcgcgc cacccgaaat gccggtcttt gcggcaacgc
     4081 ttcggccgaa ccgccctctc ggacggagcg gagtggcatt ggtcgtgggg tttgtggcgc
     4141 tgattgcaac cgtcacggcc atccccttca tcgtggcggg cgcctggccg atcggcggat
     4201 tcttcggact cgacgtggcg ctgctctacg gggccttccg catcagcagc cggcgcgcgc
     4261 aggcctacga agaggtcgtg ctcagccgtc tggaactgtg cttgcgcaag gtcgactgga
     4321 ggggccgcgc gcgggagtgg cgcttcaacc ccttctgggc gcggctcagg acgcgcgaac
     4381 aggccgagtt cggcctaacg gaccttgcca tcgtcgaaca cggccgggag gttgccgtcg
     4441 gctctttcct tggcccgcag gaaaagcagg aattcgcacg cgcactggat tgcgcgctgg
     4501 ccgatgcgcg acgctagcgc aacggccaca ggcccgcgcg cccgggcggc gtatgccctt
     4561 tcgttgccgc gaatcctgtg caagatgacg ctttccccga ctggggcaac ggctcgaagc
     4621 caactggagc acctacatga ctggctggat tctgctcgcc atcatcgtcc tgccgatcct
     4681 ttgggcgatc atgatctaca acggtctggt cgcgatgcgg cagcgtgcga accagtcctt
     4741 tgccgacatc gatgtacagc tcaagcagcg ccatgatctc atcccgaacc tggtcgagac
     4801 cgtcaagggc tatgcgacgc acgagaaaga cacgctcgac gctgtcatca aggcccgcgc
     4861 cgcagccgtt tccgcgcacg gaccacaggc gcaggggcag gccgaacagg cgctcaccgg
     4921 cgcgctcgga cggctgatcg ccatctcgga agcctatccc gacctcaagg cgaatgcgaa
     4981 tttccagcag ctccagggcg aattgtcgga catcgagaac aagctcgccg ccgcccggcg
     5041 gttcttcaac aattccgtca gcgaattcaa cgcgggaatc gaacagttcc cggccgtgct
     5101 gttcgcctcg aagttcggct tcctgccgat ggccttcttc gacgtgggcg aggaacagcg
     5161 caagacgctg gatgtcacgc ctcaggtgaa gttctgaagc ggggctgagc gatggccggc
     5221 gcgttcggcc tctacagcca catccaggcg aaccggcggc ggtcctttct gctgattgcc
     5281 ggcctgttcg cgctgatcta cgcgctggtc ttcgctggcg cgctggcggc ggccgggctg
     5341 cggggcggcc catccctgaa ctggatgctg gcgcgcgcct tcgacgacct gatcacccat
     5401 gcgccactgg ccacaggcct gacgctgatc tggatcgtga tcgcctatct gtttcaccag
     5461 aagatgatcg acgccatcac cgacagtcat tcggtggagc gcaaggacga accgaagctg
     5521 tacaaccagc tggagaacct gtgcatctcg cgcggcgtgc cgatgccgca gctcaaaatc
     5581 atggaatcgg aggtgctcaa cgccttcgcc agcggcctca acgagaagca atattgcgtc
     5641 accgtcaccc gcgggttgat gcagacgctc aacgacgagg aaatggaggc ggtgcttgcc
     5701 catgagctga cacatatccg caacggcgac gtgaagctca tggtgacggc ggtcatcatc
     5761 gccggcgtgg tctctttctt cgccgagttg ctgttccgct ggctggcgcg cggaccgacc
     5821 atccggttct ccgacgacaa cggcgattcg aagaaggctt cgcccgccgc cgccgctctg
     5881 atgatcgcgg tggtgatcgt ggtggtcgcc tgggtggcct cgggcctgct tcgcttcgcc
     5941 ctgtcgcgca cgcgcgaata tcttgccgac gccggggcgg cggaactgac gaaaaatccg
     6001 gacgcgctca tctccgcgct tttgaaaatc tcgggccggg cggagatccc gggcgctccg
     6061 tccggactga tggaaatgtg catcgagaac gagcgctcgg gcttcgtcga tgtcttcgcc
     6121 acgcatccgc cgatcgagga tcgggtcgag gcgctggtga agttcgccgg cgggcacatg
     6181 ccggcgcctg aagccgggcc aaagccgtcg ttcctgcctg aaacggtgca gctcgacagc
     6241 aagctgggtc cgtgggggca ggccgccgcc gcggccaatc cgtggcggcg ctagactcct
     6301 gaagccgggt gctcagcgta gcggcatcgc ccgctgcgcc acggctgtcc agtaggaaat
     6361 gccggcagga atcgcctcgt cgttgaagtc gaaggccgga ttgtgcagcg gcgccgtatc
     6421 gccgttgccg atgaacagga aggcgccggg cttggcttcc agcatgtagg agaaatcctc
     6481 cccgcccatg accggcggcg cgttggcgat gacgccgccc gccccggcga cggtcgcggc
     6541 ggcggccacg gcgaattccg tctcggcctg atggttcacg gtggcgggat agccatatgt
     6601 gaagtcgagc cgggcttcgg cgccaaaggc cttggccgta ttcgccacga tggcgcggaa
     6661 acgctcctcg gtcacctgac caacggcctt ttccaggaac cgcaccgtgc ctctcaacac
     6721 ggcggtttgc ggaatgacgt tgaaggcgtc gccggcgttg aaggtggtca ccgaaaccac
     6781 gacggacttg agcggatcgg tgttgcgcga ggcgatcgac tgcagcgccg tcaccatgtg
     6841 gctgccgatc agcacgggat cgatcccctg gtgcggctgc gcgccatgcg cgcccttgcc
     6901 ctcgatggtg atgacgaagt tcgcggtggc ggccatcagc gggccgggcc gggtcatgaa
     6961 atggccgacc ggcacgcccg gcacgcagtg caacccgtag aactcctgaa tgccgaaacg
     7021 gctcaccagc ccgtcatcga ccatcgcctt gccgccggca ccgccttctt ccgccggctg
     7081 gaacaccacc acgaccgtgc catcgaagcc gcggtcggcg gcaagatgct tggcagcgcc
     7141 cagcagcatg gcggtgtgcc cgtcatggcc gcaggcatgc atcatgttcg ccaccttgga
     7201 ggcatacggc aggttggtct gctcggacat gggaagagca tccatgtcgg cgcgcagcgc
     7261 cacaaccttt ccgctcgcgg tcttgcggcc cttgatcacg ccgaccacgc cggtcttgcc
     7321 aatgccggtc accacctcgt cgacgccgaa gctcttcagc ttctccgcca ccagtgccgc
     7381 ggtgcgatgc acctcgtaga gaatttcggg atgggcatgg atgtcacggc gccagctggc
     7441 aacttcgtcc ttgagagcgg cgatacgcgg atcgatggaa gacatggggc ccatagggtt
     7501 cgaggtctgg agcagcaatg ttggcgtgcg atgacggccc ggtcaacccg ccggccatca
     7561 gctagcgccc gatgcgcgcc acagggcgtc gcgggccgcc ctgttggacc agcgccgccg
     7621 ggcgggtggt ccggtattgc ccgctctcca cggcggcgag catcagcgcc gcgaccagca
     7681 tcacgcagag ccaccagacc tgcaaggccg tcggatcgat gatggcgaag gccgcgatgg
     7741 ccgtcgccac ggccagcgcc gtggctgcca cgggctgcgc caggccagcc agcgtccgga
     7801 acatcagcca gagcaccagc gccagccctg tggcgccgat cagaccgagg tcataccagg
     7861 tgtcggagat caggccgccc ggcgctcgcg gatcgaccag accggcaagg cgggcgcgca
     7921 acgccgtatc gaatccgtga ccggtgataa gccgaatggg ctgggcggca atagcgtctg
     7981 cccagtgccc gatctcggtc aggtcgtgat ccgcgcgcag ccgcatcgcc cgggccagaa
     8041 agcgggcggc gaaggccagg atcggcgccg ccagcacggc tgcgggaaat gcgatcgaca
     8101 acatgcgccc ggttgtggtc ggcatggtcc tcgcggccag aaacaccgct agggccagga
     8161 tcatcaccac ggccgcgtcg agcgagccac tcgcggctat ggcgacgccg cagacggcga
     8221 tgaggctgaa ggcctgacca tcgcgcccgc gcgtcatggt ccaggccacc gccggccaga
     8281 ccatcacacc tgcggcagcg gcggcacggc cgatggtcgg cgtttccaga tcgcgcagaa
     8341 accgcaacgc ggaagggccc agaacggctg ccacgatcag cgccaaggta gcggccgcga
     8401 cgccgatcga catgagatga agattgctgg cgcgcatgcg ctgcggaagc gcgctgacga
     8461 ccgccagcgc cagcacgcca ataccgagcg tgcgcaggag gcgctcggcg gcgggaccag
     8521 gaaaaggcgt ccaggccagc gaaagcgcac accatatgcc caatcccaga gccgccattc
     8581 cggtcggcga cagcacggca tcgcgcaggc ggtggctcag accgccactg tccctcaaca
     8641 aggccgcggc aacaatcagc agggcgccga caggcaccag aacgatgaac gcgcggcgcg
     8701 agaacagcgc ggaaaccggc gcaaccatga cgagcagtcc gaacccgaga cgcagaagca
     8761 ggcccgccgc atcggcggcg ggggattcgg gaaaccgttc ggtcttgtgt tccatcgccc
     8821 tcggacgctg cccagtcgat caaaaaatgc accttgtggg cagggaccta accgcccttt
     8881 ctgttgcaat acaattgcca tgcccgacgt atcgaccccg gacgcgcaca atacttgcgt
     8941 tcttgccaaa tctgtcacag gcaggccaca gattcgaccg ccatcgggaa ggtacagcgt
     9001 catgactaac ggggaaccgc tctatgccag cctgctggcg cgccggtctg caaaggccaa
     9061 ggacctcatc gcgcccggtc ctgacagcgg acagattgaa cgcatggtcc ttgccgcatc
     9121 gcgggcgccg gaccacggtc gactcgtgcc gttccgcttc gtcatgatcg gggaagaccg
     9181 acggacggcc ttcgccaccg ttctggccgc agcggcaaag caggagaacc cgggactttc
     9241 tgcgtccgag gttgaccgcg cgcgcgacaa ggccttcgag ggaccatgcc tgatcgcgat
     9301 gattgcccgc gtcgacgtcg cgcatcccaa gatcccggcc agcgaccagt ggttgacggc
     9361 gggctgcgcg ctggagaatc tccttctggc ggcgcaggcg ctgggtttcg cggtggcggt
     9421 ccggtccggg cgctatttgg agatggcggt ggtgcgagag gcgttcgcgc tcgcgcctgt
     9481 cgagcacttc gtctgcttcc tggccgtagg aacgccggcg gactatccgc cggcgaagcc
     9541 aaagccttcc ctggatcagg tcctgtcgcg ctggtaagtc agctctgctt gttgcgtttg
     9601 cggctttctt ccatgtagcg cagcaggtcg accgtgccgt cgtaggcctt ggtgtcgatg
     9661 atctcccagg tcttgccctt ggtgtcgtag atgcgctcga aggcggaacg gtcgcgcatc
     9721 ggcgcttcca gcatggcgcg acgcagcatc gccttcaggt cctccggcat gtcggcgagg
     9781 taggcgatcg gcgagttcac cagcggctcg gacttcagca cgatgcggaa gtcatcgcgc
     9841 cgcatgggcg agccgtcgac attcttgagc acgccgcgcg cgagcagacg ggtcagggtg
     9901 gaatcctcgt ccgacttcca ttccccgacc gcgagatcga caagcccctg cgaaagcgcg
     9961 acgagcgcgt tttcatggct gcccgtgaag acctgcttgc cgaggaagtt gtcgggatca
    10021 agcttctgcg aggccagcgc gaagcgcgcc acggaatagc cggtcagcga attgggatcg
    10081 accagtccga tgctcctgcc ctttaggtcc tcgaccttcg ggttcgggcc acgcgccatg
    10141 gcatagacca cggaatgggc gccgcgcccg ccgtcggcgt tggtctccac cgcaaaggcc
    10201 tcgatcttcg agcccgcgag ccgcgccttg gcgaagaaca gcgggctgta gatcgccatg
    10261 tggatcaggc cggccttttg gctttccagg agcgcctggt agtcgttggc gaccttcagg
    10321 ctgatcttca gaccgatctc acgcgacagg taggtgatca gcggcgacca acggtcgatc
    10381 tgctccttgg agaactcctt gggcacgatg ccgaggttga tctcggggta tttagccgac
    10441 agttcgagca acttgggatc gggctcgcga tagtcgagga agttacactc gaccgccctg
    10501 aacaggatgg cccggcagtc gatgcgggcc tggatctccg gtcccttctc cttgaggcgc
    10561 gggtcgagca ccttctggtc cggtgtcttg ttgtcctgcg acttgtagtc ggtcgtgcgc
    10621 gtgtcgaccg gcttgtagtc gaggggtcgg aagtcgaacg gggcacgatc cgggatccgg
    10681 tagtcgacgt agcggttctc gaccacaacc ggcgtctgga cgcgaatctc cgacgggttc
    10741 acgcgccgct ccggcggcag ctgatcgcgc aggttcggca agaaaggcgg cggacttccg
    10801 agcatttgcg tgcgcatttc cggcgtgggg gtgcgcagtt cggtcgccgg gcgggcatcg
    10861 ccgcgtgcct cgatcgccgg acgcggagct gcgc
//
LOCUS       DAOMXN010000066        10876 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_3237_length_10876_cov_13.763700, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000066 DAOMXN010000000
VERSION     DAOMXN010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 10876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10876
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3237_length_10876_cov_13.763700"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /locus_tag="PK812_06635"
     CDS             complement(<1..922)
                     /locus_tag="PK812_06635"
                     /inference="COORDINATES: protein motif:HMM:NF006179.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta
                     family protein"
                     /protein_id="HRJ69269.1"
                     /translation="MTNDRQDGPARAIRIAILAMGGEGGGVLANWLIDLAETNGHVAQ
                     MTSVPGVAQRTGATIYYIEMFPEALIQPGQSPVLAQMPMPGDVDLVIASELMEAGRAI
                     QRGIVTPDRTTLIASSHRVFAMTEKIALADGRVDEKALLLACRTAAKHFVSFDMMQMA
                     ERAGSVVSAVLLGAVAGARALPFSRDQFEQTIERGGVGVAASKRAFSAAFDAGSGRMI
                     PAAAAPQPAVAPSGAATSSQQTERLIIGAMTRFPPEAMEIIRSGIEQCVDWQDVANAK
                     LYLQRMEPILELDRRIGDGGWCRKGMPEQDC"
     gene            complement(944..3112)
                     /locus_tag="PK812_06640"
     CDS             complement(944..3112)
                     /locus_tag="PK812_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HRJ69270.1"
                     /translation="MAERSFKEEVKSLRLGAGEVFRGEGILAITKALLESGVSYVGGY
                     QGAPISHLMDVLADAEDILNDLGVHYESSASEATAAAMLAASVNYPLRGACAFKSPVG
                     TNVASDALANLSSGGVMGGAMIIVGEDYGEGSSIMQERSHAFAMKSQIWLMDPRPNLP
                     KIVECVHKGFELSEASNTPVMLEVRIRTCHVTGRFNTQDNVRPRFTRRDALENPVRDV
                     NRIVLPPASYLHEKEKLEKRWPAAQAYIERSGINEMFDGDLKDVGIVMLGGMYNSTMR
                     ALERHGLADAFGNARVPIYVLNVAYPLVDNELLRFASGKKALLIVEEGAPEYIEQAVS
                     TMLRRADLQTRVHGKDMLPMGGEYTALVLRDGIGRFMKSYYPSLVVAASAAPGDNRVK
                     AALAALDGQIHGRPPSFCTGCPERPIFTAMKLVERELGPHHVACDIGCHLFSILPPFN
                     IGATTMGYGLGGASLAAFNMRAEKEPTKRAIAVMGDGGFWHNGLLSSVGNAVFNKSDN
                     VMIVVDNGYSAATGGQDILSSHHPNADRSTMNSIEKAVRGVGVTWARTLTRTYDLKAM
                     TATLKEALTTDAKGPKVIVAQSECMLNKQRRERPLVNRAIGDGKRVVRERFGVDPDTC
                     TGDHSCIRLSGCPSLTVAPHPNPLRKDPVTKVINSCVGCGLCGEVSHAAILCPSFYKA
                     SIINNPDPADSWKHRWRTRIIGFLQRRHARRNARLQAVSG"
     gene            complement(3271..4509)
                     /locus_tag="PK812_06645"
     CDS             complement(3271..4509)
                     /locus_tag="PK812_06645"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRJ69271.1"
                     /translation="MVLFAVILVNFTLTRPSPVDIAFALALAMTPFCAQKMTRRGIVF
                     LALIVAWWTGMMVSSWPHSYDEDVVYQLTKTSYAISIGITACLVSMHWTRDQVERLFQ
                     FWIFSASIAATLATIGFALQIEDLVWDGRGKAFLDDPNMYAAFVLPAFLGCIYFILTG
                     RNRKLYSALLGLMVVGLLVAFSRVAIAAALGTGLALTVILNRRLLVKTSTALFIVGAL
                     AVAVGAVAILSFPEFAEKLADRATLAKDYDSGETGRLARYLKSFNLMLDHPEGMGVLQ
                     FEKIFPEPIHNFWLGSFVNYGWLAGFAWTFIVYFGLALTIRNYRHTGDNLMLLVFLSW
                     LAIFGCALLHEAERWRHLWFFTGIMWGVTARNWPQRARVPEPVRAGTAGPQAMPVPSA
                     LRHQQIPLHGVRGSATGTLT"
     gene            4870..5316
                     /gene="aroQ"
                     /locus_tag="PK812_06650"
     CDS             4870..5316
                     /gene="aroQ"
                     /locus_tag="PK812_06650"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HRJ69272.1"
                     /translation="MSRLVFILNGPNLNLLGKRQPHIYGAETLADVEAECRALAAQHG
                     IDIRFHQSNREYEIIDWIHEARDIAGGIIINPGAFTHTSIAILDALNTFDAPVIEVHI
                     SQVHKREAFRHHSYVSHRADAVMAGFGTQGYQLALLRLVRLIDQKK"
     gene            complement(5375..7063)
                     /locus_tag="PK812_06655"
     CDS             complement(5375..7063)
                     /locus_tag="PK812_06655"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HRJ69273.1"
                     /translation="MERIWLKHYPEGVPADIDVAQYKSVVELFEESFQKYRDRKAYAF
                     MDKFMTYGDLERHSAALGAYLQSRGLQRGDRVAIMMPNILQYPVAVAAVLRAGFVVVN
                     VNPLYTPRELEHQLKDSGAKAIIVVENFASTLQQCIAKTPVKLVITTGIGDMLGFPKS
                     MIVNFVLKKVKKVVPAFSLPGAVSFNAALAAGRGMTLAKAATGPDDVAFLQYTGGTTG
                     LSKGATLLHRNIVANVLQNEAWLQPALKKGTPVEQWNVVTALPLYHIFALTACCLLGM
                     RTGAMLILIPNPRDLPAFIKELAKYEVHMFPAVNTLYNGLLNNEEFRKLNMRSLRASN
                     GGGMAVQRAVAEGWFKQTGCPVIEGYGLSETSPTLTCNRADNDEYTGTIGLPVPNTII
                     SIRDDNGQEVKLGEAGEICAKGPQVMAGYWNHPEETAKVMTADGYFKTGDIGIMDEKG
                     QVKIIDRKKDMVLVSGFNVYPNEVEDVVAMHPGVLECAVIGVPDKSSGEAVKLFVVKK
                     DPNLTEKDLADYCSKNLTGYKKPKYIVFRSDLPKTNVGKILRRELRDIDQKSTG"
     gene            7301..10543
                     /locus_tag="PK812_06660"
     CDS             7301..10543
                     /locus_tag="PK812_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HRJ69274.1"
                     /translation="MAAGNPLDPIPHPPRKPYFGNLLSLDPNAPVQDLNKLAKELGPI
                     FWLDMQGYPLIFVSGADLVDELSDEKRFDKAVKGSLKKVRAIAGDGLFTAYTEEPNWG
                     KAHNILLQPFGMRAMQSYHPAMVDIAEQLILKWERMNAEDEIDVVHDMTALTLDTIGL
                     CGFDYRFNSFYRLDYHPYVEALVRSLETVMMMRGLPWERFTLAKREKQIAEDIAFMNQ
                     MVDAIVAERRKAGDAAEGKKDLLNAMIAGVDRVSGQQLDDVNIRYQINTFLIAGHETT
                     SGLLSYTLYALLKNPEILKKCYDEVDRVLGSDVSQKPTYQQVTQLTYITQVLKESLRL
                     WPPAPAFGLHPYKDEVIGGKYRLKKGSFINILVGALHRDPKVWGPNPEVFNPDNFSRE
                     AEAARPVNAWKPFGNGQRSCIGRAFAMHEAALAIGMILQRFKIIDHKRYQLHLKETLT
                     VKPEGFTIQVRARSDRDRSVTGPAPSITTATALQKSATLPTARPGHNTPLLVLYGSNL
                     GTAEELAHRVADLATLNGFDTRFAPMDEFAGNLPEQGGVVIFCSSYNGAAPDNATQFV
                     KWIQSDLPRDALAKVRYAVFGCGNSDWAATYQSVPRMLDERMADLGGKRVAPRGEGDA
                     RTDLDSHFETWFGALRPLAAKEFGIETTFTAEAGAGQPLYTIETVAPAIPNIVASTAG
                     AKAMKVMANRELQNREGANASDRSTRHIEVALPEGVTYRVGDHLSIVPRNDLALAEAV
                     ARRFGLTLSETIRLQVPEGRRSQLPAGQPISVGRLLTEFLELQQVATRKQIQTMADHT
                     RCPMTRPKLAAYVGEDAAAQEKYRAEVMAKRKSVFNLLEEFPACELPFNVYLEMLSLL
                     SPRYYSISSSPMVDSGRCSVSVGVVEGPALSGRGTYRGVCSTFMANRVAGDTIHATIK
                     ETKAGFRLPDDAQVPIIMVGPGTGLAPFRGFLQERAALKAKGRTLGPSLLFFGCRHPE
                     QDFIYGEELKAFEKEGITGLHVAFSRSGKKKTYVQDLIKAQKDGVWDLIEKGAIVFIC
                     GDGSKMEPDVKAALMAIYAEKTGSSEAQAESWIEKMGAENRYVLDVWAGG"
     gene            complement(10598..>10876)
                     /locus_tag="PK812_06665"
     CDS             complement(10598..>10876)
                     /locus_tag="PK812_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRJ69275.1"
                     /translation="GLGRQVAESAGVGRQLVAIFASGSRRERLPGVAAPTLVIHGARD
                     PLVPIGHGEIVHQLVPAATMLRLDDMGHALSRPHWDTVIDAITAHAGR"
BASE COUNT         2013 a         3408 c         3464 g         1991 t
ORIGIN      
        1 ggcaatcctg ctcgggcatg cccttgcggc accagccgcc gtcgccgatc cgccgatcca
       61 gttcgaggat cggttccatc cgctgcaggt acagctttgc attggcgacg tcctgccaat
      121 caacgcactg ctcgatgccg gatcggatga tctccatcgc ttccggcggg aagcgggtca
      181 tggcgccgat gatcaggcgt tcggtctgtt gtgacgatgt cgctgcaccg ctcggcgcga
      241 cggccggttg cggtgcggcc gcagcaggga tcatgcgccc ggagccggca tcgaacgctg
      301 ccgagaaggc acgtttcgaa gcggcgacgc caacaccgcc gcgttcgatc gtctgctcga
      361 actggtcgcg ggaaaacggc agcgcccgtg cgcctgcgac ggcgccgagc aaaacggcgg
      421 agacaacgct cccggctctc tcggccatct gcatcatgtc gaaggaaacg aaatgcttcg
      481 ctgccgtccg gcaggcgagc agcagggcct tctcgtcgac gcggccatcg gccagggcga
      541 ttttttccgt catggcgaag acgcgatgcg aggaggcgat caacgtcgtc cggtccggcg
      601 tgacgatgcc gcgttgtatg gcgcggccgg cctccatgag ttcggaagcg atcacgaggt
      661 cgacgtcgcc gggcatgggc atctgcgcca ggaccggtga ctggcccggc tgaatcaacg
      721 cctcgggaaa catctcgatg tagtagatcg ttgcgccggt ccgctgcgcc acgccgggaa
      781 cggacgtcat ctgggcgacg tggccgttgg tttcggccaa gtcgatcagc cagttggcga
      841 gtacgccgcc tccttcgcca cccattgcaa gaatggcaat gcggatggcg cgtgccggac
      901 cgtcctgcct gtcgttcgtc atgtcgcgca gagccttccg ccgtcagcca ctgaccgcct
      961 gcagacgcgc gttgcggcgc gcatgccggc gctggaggaa gccgatgatg cgtgtgcgcc
     1021 atctgtgctt ccaactgtcc gccgggtcag gattgttgat tatcgaggcc ttgtagaaag
     1081 acgggcaaag gatcgccgcg tggctgacct cgccgcacag gccacacccg acgcaggaat
     1141 tgatcacctt ggtgactggg tccttgcgca ggggattggg atgtggcgcc acggtgaggg
     1201 aaggacaacc ggacagcctg atgcaggagt ggtccccggt gcaggtgtcc gggtccacac
     1261 cgaagcgctc ccgcaccacg cgcttgccat cgccgatcgc gcggttgacg agcggacgct
     1321 cgcgccgctg cttgttgagc atgcactccg actgggcgac gatgaccttc ggccctttcg
     1381 cgtcggtggt cagcgcttcc ttgagcgtcg cggtcatcgc cttgagatcg taggtgcggg
     1441 tcagcgtccg cgcccaggtc acgccgacgc cacgcaccgc cttttcgatc gaattcatcg
     1501 tggagcgatc ggcgttggga tggtgggacg acaggatgtc ctgaccgccg gtcgctgcgg
     1561 aatagccgtt gtccacgacg atcatcacgt tgtcggattt gttgaagacc gcattgccga
     1621 cggacgacag caggccgttg tgccagaagc cgccgtcacc catcacggcg atggcgcgct
     1681 ttgtcggctc cttctccgcg cgcatgttga acgcagcgag gctggcacca ccgagcccgt
     1741 agcccatggt tgtcgcaccg atgttgaagg gcggcaggat cgagaacagg tggcagccaa
     1801 tgtcgcaggc cacgtggtga gggccaagct cgcgttccac cagtttcatc gcggtgaaga
     1861 tcggacgctc cgggcagccg gtgcagaagg agggtgggcg gccgtgaatc tggccgtcca
     1921 gggctgcgag cgccgccttg acgcggttgt cgccaggcgc tgccgaagct gcgacaacca
     1981 agctcgggta gtagctcttc atgaagcgcc cgatgccgtc gcgcagcaca agggcggtat
     2041 actcgccgcc catcggcagc atgtccttgc cgtgcacacg agtctgcagg tcggcccgcc
     2101 gcagcatggt gctgacggcc tgctcaatgt actccggagc gccttcctcg acgatcagca
     2161 gcgccttctt tccggacgcg aagcgcagca gttcgttgtc gaccagcgga taggcgacgt
     2221 tgaggacgta gatcggcacg cgcgcattgc cgaaggcatc ggccagaccg tgccgttcca
     2281 gcgcacgcat ggtcgagttg tacatgccgc caagcatgac gatgccgaca tccttgaggt
     2341 cgccatcgaa catctcgttg atcccactgc gctcgatgta ggcctgagcg gcgggccagc
     2401 gtttctccag cttttccttc tcgtgcagat agcttgctgg cggcaagacg atacgattga
     2461 catcgcgcac cgggttttcg agtgcgtcac gacgggtgaa cctgggccgc acattgtcct
     2521 gcgtgttgaa ccggcccgtg acatggcagg tcctgatacg gacttcgagc atcaccggcg
     2581 tgttcgacgc ctccgacagt tcgaaaccct tgtgcacgca ttccacaatc ttgggcaggt
     2641 tgggccgggg gtccataagc cagatctggc ttttcatcgc gaaggcatgg ctgcgctcct
     2701 gcatgatcga ggagccttcg ccgtagtctt ccccgacgat gatcatggcc ccgcccatga
     2761 cgccgcccga ggacaggttg gccagggcat ccgaggccac gttggttccg accggagact
     2821 tgaaggcgca ggcgccgcgc aaggggtagt tgacagaggc cgccagcatc gccgcagccg
     2881 ttgcctcgct ggcgctggat tcgtagtgaa cgcccagatc gttgagaatg tcctcggcgt
     2941 cggcgagcac gtccataaga tgcgaaatcg gagcgccctg gtagccaccg acgtaggaaa
     3001 cgcccgactc caacagggcc ttggtgatgg ccaggatacc ttcgccgcgg aatacctcgc
     3061 cggcacccag ccgcagcgac ttcacctctt ccttgaatga acgctcagcc atgttccccc
     3121 gaaactccga tgttcgcctc ggggttcgat cgggcgcagc gccccgttcg aacctcccga
     3181 ggggctcgaa atcgtgtgcg gtgttgtaac agtcacgcgt ccgccggcgc aagccgcacg
     3241 gccccggcgc tttcggcctg gaacgtcacg ttatgtcagg gtgccggtcg cagagccacg
     3301 gacgccatga agagggatct gctggtgacg cagcgcggag ggaaccggca tggcctgcgg
     3361 cccggcggtc ccggctctga ccggttcggg cactcgcgcg cgctgaggcc aattgcgcgc
     3421 ggtcacgccc cacatgatgc cggtgaaaaa ccagagatga cgccagcgtt cggcctcatg
     3481 cagcaaggcg catccgaaga tcgcgagcca ggacaggaag accagcagca tcaggttgtc
     3541 gccggtgtgg cgatagttgc ggatcgtcag agccaggccg aagtagacga tgaaggtcca
     3601 ggcgaagccc gcgagccatc cgtagttgac gaaggaaccg agccagaaat tgtggatggg
     3661 ttcggggaag attttttcga actgaaggac gcccatgccc tccggatggt cgagcatcag
     3721 gttgaaggat ttgaggtaac gggcaaggcg gcccgtttcg ccggagtcgt agtccttcgc
     3781 cagcgttgcg cggtcggcga gtttctcagc gaattccgga aagctgagaa tggccaccgc
     3841 gcccaccgcg accgcgaggg cgccgacgat gaacagggcg gtcgaggtct tgaccagcag
     3901 cctgcggttc aagatcacgg tcagggcgag gcccgtgccg agcgccgccg caatggcaac
     3961 gcgcgagaag gcgaccagaa gcccaaccac catgaggccg agcagggcgg aatagagctt
     4021 tcggttccgc ccggtcagga tgaaatagat gcagcccaga aaggccggca gcacaaaggc
     4081 cgcatacatg ttgggatcat cgaggaatgc cttgccgcgc ccgtcccaca ccaggtcttc
     4141 gatctgaagc gcgaagccga tggtggccag cgtggcggcg atactggcgg aaaatatcca
     4201 gaactggaac agccgttcga cctggtcgcg cgtccagtgc atgctgacca ggcaagccgt
     4261 gatgccgatg gagatcgcat agcttgtctt cgtcagctga tagaccacgt cctcgtcata
     4321 gctgtgcggc cacgacgaca ccatcatccc ggtccaccac gcgacgatga gcgcgaggaa
     4381 gacgatgccg cgccgcgtca tcttctgcgc acagaacggc gtcatggcga gcgccagggc
     4441 aaaggcgata tcgaccggcg aagggcgggt cagcgtgaag ttgacgagga tcacggcaaa
     4501 caggaccacg ccagtgcgta gcttcctgaa ccgctcgccg atttggcgga acgctgcccg
     4561 acccatgatt ccaagacctc tcttcccgtc ctggccgtga cagcaaagcc gtatgcctgc
     4621 gccggacgat cctgaccgac gaccctagaa cgcgttcgtt aagctctggc taaccatgcg
     4681 gcgacctggt tcgcggcaca tgcggcgccc ggcttgctca aaaggcattc ctttatgctt
     4741 cttccaccct ataggatcgt ataaactttt gatatatatg ggtgatatgc gcttgcgccg
     4801 tcgccttgtg tctcccgcct cattcccgtc aggtcatgcg atgacgcgat gggtcactat
     4861 aggaacgcca tgtcccgact cgtgttcatt ctcaacggtc cgaacctgaa cctgcttggc
     4921 aagcgccagc cccacatcta cggggcggag acgttggctg acgttgaggc ggagtgccgg
     4981 gcccttgccg cgcagcatgg tatcgacatc cgatttcatc agtccaaccg ggaatacgaa
     5041 atcatcgact ggattcatga ggcgcgggac atcgccggcg gcatcatcat caatcccggc
     5101 gctttcaccc atacgtcgat tgccattctt gatgcgctca acaccttcga tgcgccggtc
     5161 atcgaagtcc acatctcgca ggtgcacaag cgcgaggcgt tccggcatca ctcctacgtc
     5221 tcgcaccggg cagatgcggt gatggccggt ttcggcacgc agggctacca gctggcgctg
     5281 ctgcgcctgg tccggctgat cgatcagaaa aagtgacggg gcccgaaggc cccgccgctg
     5341 aacgccggtc caagtatcgg gcttcaggct gacatcagcc tgtggacttc tgatctatgt
     5401 cgcgcagttc gcgacgcagg atcttgccga cgttcgtctt cggcaggtcg ctgcggaaga
     5461 cgatgtactt cggcttcttg tagcccgtga ggttcttgga gcagtagtcc gccaggtcct
     5521 tttccgtcag gttcggatcc ttcttcacga cgaacagctt tacagcctcg cccgagctct
     5581 tgtcgggcac gccgatcacg gcgcattcga gaacgccggg gtgcatcgcc acgacgtcct
     5641 cgacctcgtt cggataaacg ttgaagccgg acacgagcac catgtccttc ttgcggtcga
     5701 tgatcttgac ctggcccttc tcgtccatga tgccgatatc gccggtcttg aagtagccgt
     5761 cggccgtcat gaccttcgcg gtctcttccg gatggttcca gtaaccggcc atgacctgcg
     5821 gacccttggc gcagatctcg cccgcctcgc ccagcttgac ctcctggccg ttgtcgtcac
     5881 ggatcgagat gatggtgttg ggcacgggca ggccaatcgt gccggtatac tcatcattgt
     5941 cagcccggtt gcacgtcagc gtcggcgaag tctcggagag gccgtaacct tcgattacgg
     6001 gacagccggt ctgcttgaac cagccttcgg ccaccgcccg ttggaccgcc atgccgccgc
     6061 cgttcgaggc gcgcagcgaa cgcatgttga gcttgcggaa ttcctcgttg ttcaacagcc
     6121 cgttgtagag cgtgttgacc gccgggaaca tgtgcacttc gtatttcgcc agttccttga
     6181 tgaaggccgg cagatcgcgc gggttgggaa tcaggatgag catcgcgccg gtacgcatgc
     6241 cgagcaggca gcaggcggtc agcgcgaaga tgtggtacag cggcagcgcg gtgaccacgt
     6301 tccattgctc taccggcgtg cccttcttga gcgccggctg gagccaggcc tcgttctgga
     6361 gcacgttcgc gacaatgttg cggtggagca gggtggcgcc tttcgacagg cccgttgtgc
     6421 cgccggtata ctggaggaag gcgacatcgt ccggtcccgt cgcggctttc gccagcgtca
     6481 tgccccggcc ggccgcgagc gcggcgttga acgataccgc gccgggcagg ctaaaggccg
     6541 gcactacctt cttcaccttc ttcaggacga agttgacgat catgctcttg gggaagccca
     6601 gcatgtcgcc gatgccggtg gtgatgacca gtttcactgg tgtcttggca atgcactgct
     6661 gcagcgtgct ggcgaagttc tcgacgacga tgatcgcctt cgcgccggag tccttgagct
     6721 ggtgctcaag ctcgcgcggc gtgtagagcg ggttgacgtt gaccacgacg aagccggcgc
     6781 gcagcacggc ggcgaccgcc accgggtatt gcaggatgtt cggcatcatg atggcgacgc
     6841 gatcaccccg ctgcaggccg cgcgactgca ggtaggcgcc gagggccgca gagtggcgct
     6901 caaggtcccc gtaggtcatg aacttgtcca tgaaggcgta ggccttccgg tcgcggtact
     6961 tctggaagct ttcctcgaaa agctcgacga ccgacttgta ctgcgcgacg tcgatgtcgg
     7021 cgggcacacc ctcgggatag tgcttcagcc agatgcgctc catggaacag ccctttctcg
     7081 tttccttgtg tcgttcttgt cgtctgtatg cacatgtcgg tccgctcagg cttagagtgg
     7141 ccagagtggc gcaaaaatca actggcaatg gaaaaatgcc gcctgtcgca ttgcgagccg
     7201 cgcttggcgc ggcctataca ggacttgcgc gttcctccgt ctttcgatta agaacggttc
     7261 atatctaaac taaattcacc gcgcaagctg ggagtgaggc atggccgccg gaaatccgct
     7321 cgatccgatc ccgcatccgc cgaggaaacc gtatttcggc aacctgcttt cactcgatcc
     7381 caacgcgccc gttcaggacc tgaacaagct ggcgaaggaa ctcgggccga tcttctggct
     7441 cgatatgcag gggtatccgc tgattttcgt gtccggcgcc gatctggtgg acgagctgtc
     7501 cgacgagaag cgtttcgaca aggccgtgaa gggctcactg aagaaggtgc gtgccatcgc
     7561 cggcgatggt ctctttactg cctacaccga ggagccgaac tgggggaagg cccataacat
     7621 cctgctgcag ccgttcggca tgcgggcgat gcagtcctac cacccggcca tggtcgacat
     7681 tgccgagcag ctcatcctga agtgggagcg catgaacgcc gaagacgaga tcgacgtcgt
     7741 gcatgacatg acggcgctga cgctcgacac tatcggcctg tgtggcttcg actatcgctt
     7801 caattctttc taccggctcg actatcaccc ctatgtcgag gcgctggtgc gctcgctgga
     7861 aacggtgatg atgatgcgcg gcctgccgtg ggagcggttc acgctcgcga agcgggagaa
     7921 gcagatcgcc gaggatatcg ccttcatgaa ccagatggtg gacgccatcg tggccgagcg
     7981 gcgcaaggct ggcgatgcgg ccgaaggcaa gaaagacctg ctgaacgcca tgatcgccgg
     8041 tgtggaccgc gtgtccggcc agcagctcga cgacgtcaac atccgctacc agatcaacac
     8101 attcctgatc gccggccacg aaaccacgtc cggactgctg agctacacgc tctacgcgct
     8161 tctcaagaac ccggagattc tcaagaagtg ctatgacgaa gtcgaccgcg tgctcggctc
     8221 ggatgtgtcg cagaagccga cctaccagca ggtgacccag cttacctaca tcacgcaggt
     8281 gctgaaggag tccctgcggc tgtggccgcc ggcgcccgcc ttcggccttc atccttacaa
     8341 ggacgaagtc atcggcggaa agtaccggct caagaaaggc tcgttcatca atatcctggt
     8401 tggggcgttg caccgcgatc caaaggtctg gggcccgaac cctgaggtct tcaatcccga
     8461 caatttcagc cgcgaggctg aggccgcacg cccggtcaac gcctggaagc cgttcggaaa
     8521 cggacagcgt tcctgcatcg gccgcgcctt cgccatgcat gaagcggcgc tggccatcgg
     8581 catgatcctg caacggttca agatcatcga ccacaagcgc taccagctgc acctgaaaga
     8641 gacgctgacc gtgaagccgg aaggtttcac catccaggtc cgggcgcggt cagaccggga
     8701 tcgcagcgtt accggtccgg cgccatcgat cacgaccgcc accgcattgc agaagtccgc
     8761 cacgctgccg accgcgagac ccggccacaa cacgccgctg ctggtgctct acggctccaa
     8821 tctcggcacg gccgaggaac tggcgcaccg cgtcgctgac ctcgcgacgc tgaacgggtt
     8881 cgatacgcgt ttcgcgccga tggacgaatt cgcgggcaac ctgccagagc agggtggcgt
     8941 ggtgatcttc tgttcatcct acaacggggc ggcgccggac aacgccacgc aattcgtcaa
     9001 gtggatccag tcggacctgc ccagggatgc gcttgcgaag gtgcgctacg cggtgttcgg
     9061 ctgcggcaac agcgactggg ctgcgaccta ccagtccgtg ccgcgcatgc tcgatgaacg
     9121 catggcggac ctaggcggca aacgcgtcgc gccgcgcggc gaaggggatg cccgaactga
     9181 tctcgacagc catttcgaga cctggttcgg cgcgttgcgg ccgcttgcgg ccaaggagtt
     9241 cggcatcgag accacgttca ccgcggaggc cggcgcgggc cagccgcttt acacgatcga
     9301 gaccgtggct ccggcgatcc ccaacatcgt tgcctcgacc gcaggcgcca aggcgatgaa
     9361 agtgatggcc aaccgggaat tgcagaatcg cgagggggcg aatgcatccg accggtccac
     9421 gcgccacatc gaagtggcgc tgccggaagg cgtcacatac cgtgtgggcg accatctctc
     9481 catcgtgccg cgcaacgatc tggcgctggc cgaggccgtg gcgcgtcgct tcggtctcac
     9541 cctctccgag accatccgcc tgcaggttcc ggaggggcgg cgctcgcagc ttccggccgg
     9601 ccagccgatt tccgtcggtc ggctgctgac ggagttcctc gaacttcagc aggtggcgac
     9661 acgcaagcag attcagacca tggcggatca cacgcgctgc ccgatgacca ggccaaagct
     9721 tgccgcctat gtcggtgaag acgccgcagc gcaggagaag taccgcgccg aggtcatggc
     9781 gaagcgcaag tcggtgttca acctgctgga agaattcccg gcctgcgaac tgccgttcaa
     9841 cgtctatctg gaaatgctgt cgctgctctc gccgcgctac tattcgatct cgtcctcgcc
     9901 gatggtcgat tcgggcaggt gcagcgtttc cgtcggcgtg gtggaaggtc cggccctgtc
     9961 agggcggggg acctatcgcg gcgtctgctc gactttcatg gcgaaccgcg tcgccggcga
    10021 tacgatccac gccacgatca aggaaaccaa ggccgggttc cgcctgccgg acgatgcgca
    10081 ggtgccgatc atcatggtcg gtcccggcac gggactggcc ccgttccgcg gcttcctgca
    10141 ggaacgcgca gcactcaagg cgaagggcag gacgctcgga ccgtcgctgc tgttcttcgg
    10201 ctgccggcat cccgagcagg acttcatcta tggcgaggag ctgaaggctt tcgagaagga
    10261 aggaatcacc ggcctgcatg tcgccttttc ccgctccggc aagaagaaga cctacgttca
    10321 ggatctgatc aaggcgcaga aggacggcgt ctgggacctg atcgagaagg gcgcgatcgt
    10381 gttcatctgc ggcgacggct ccaagatgga accggacgtc aaggcagcgc tgatggcgat
    10441 ctacgccgag aagaccggct caagcgaagc gcaggccgaa tcctggatcg agaagatggg
    10501 tgcggagaac cgctacgttc tcgatgtctg ggcgggcggg tgatgtcctg acccttacgc
    10561 gcattccggc agggacagga ccatgcgacg ccgggagcta cctcccggcg tgcgctgtaa
    10621 tcgcgtcaat cactgtgtcc caatgcgggc gcgaaagcgc atggcccatg tcgtcgagcc
    10681 gcaacattgt ggcagccggc acgagttgat gcacgatttc tccgtgtcca atcggcacca
    10741 gcggatcacg cgcgccgtgg atcaccaacg tcggcgccgc cacgccgggc aggcgctcgc
    10801 ggcggctgcc tgacgcgaag atcgccacga gctgccgacc gacaccggcg gactcggcga
    10861 cctgccggcc gagccc
//
LOCUS       DAOMXN010000067        10821 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_3249_length_10821_cov_12.545142, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000067 DAOMXN010000000
VERSION     DAOMXN010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 10821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10821
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3249_length_10821_cov_12.545142"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="PK812_06670"
     CDS             <1..417
                     /locus_tag="PK812_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010687123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HRJ69276.1"
                     /translation="EAHGLALVEFTARSREAPTAETIRAARAMFDTLAYPAVLHCKSG
                     ADRAGFMAALYLILRENARVDEALGQLSARYGHWRWSKTGILDAFLERYRDEGESKGI
                     GLIDWATHHYDPARLTAEFRPSFWSNFVVDRILRRE"
     gene            complement(404..2140)
                     /locus_tag="PK812_06675"
     CDS             complement(404..2140)
                     /locus_tag="PK812_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003600608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ69277.1"
                     /translation="MRRLWAGWVRPHRWTLLAIAAAIGTIAIMSALYPYLIKTIFDAY
                     DTQNKRLVQWTPLFVILVTSVRGMAMYAQTTLTNRVVSQVEADLQTTLYKHLIDADIA
                     QLSRETPAALTQRFTTDFAFIREALTRISTVFLRDVTTGVAVLGVMFWIDWQVTLCAL
                     VVLPIVVPPLNKIGKKLRRTALSTQEQTGQMASMVAESLSGARVAKTYGLEAYLKDKA
                     ATAFDTVRALKVKAANQRARMDPLLEIAGGLAVSGVLIFIGWRIVRGQSTIGDFTGYV
                     TAILLAAQSLRSLGNLNAIVQEALAALTRLFAVIDEPPGVSEQPGAGALEVSRGEVRF
                     DAVSFAYAGSGLALNGVDFVAPAGKTTAIVGRSGAGKSSLLALAPRLFDPTGGRVLID
                     GTDIRDVTLASLRAKIATVSQDIVLFDDTVRANILFGRPGAREDEVIAAARAAAAHDF
                     ILALPQGYDTMVGDRGGRLSGGERQRIALARAFLRDAPILLLDEPTSALDAQSEDLVQ
                     KALADLMRGRTTLVVAHRLATVRAADLIVVMEAGRVVESGTHESLLKADGAYAGFHRL
                     QFGDAGGALTRA"
     gene            complement(2320..2394)
                     /locus_tag="PK812_06680"
     tRNA            complement(2320..2394)
                     /locus_tag="PK812_06680"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2360..2362),aa:Gly,seq:gcc)
     gene            2495..3016
                     /locus_tag="PK812_06685"
     CDS             2495..3016
                     /locus_tag="PK812_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019965362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HRJ69278.1"
                     /translation="MNDHETTDISAPLLAFLDRLGIETRTMDHAPVFTVAESQALRGA
                     IAGAHSKNLFVKDKKGRLFLVTALEDASLDLKRLHEAIGAQGRVSFASADQLREHLGI
                     EPGSVTPFAVMNDRAGAVTMVLQEALMRHDVQNFHPLTNRRTTTIRRADLVRFLEAAN
                     HPPRIVALPEAAK"
     gene            3128..4024
                     /gene="trxA"
                     /locus_tag="PK812_06690"
     CDS             3128..4024
                     /gene="trxA"
                     /locus_tag="PK812_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012454104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRJ69279.1"
                     /translation="MMQAGGTATDALIRETTTAAFRQDVIAESAKQPVLVDFWAPWCG
                     PCRQLTPVIEKVVKAAGGKVKLVKMNIDDHPQVAGQMGIQSIPAVIAFSKGQPVDGFM
                     GALPESQVKTFIERLVGPLGAGGTAELLKQAAAAAESGDHPGAAELFAAVLAQEPENL
                     QALGGLAKVQLDAGSPEQARRLIDTVPPAKHADPAIASVLAALELAERAASLGDLVDL
                     ERRIAVDPADHQARFDLAVALNSKGFKAEAADHLLEIIRRDRSWNEDGARKQLLQLFE
                     AWGPMDEVTIGARRKLSAYLFR"
     gene            4045..4713
                     /locus_tag="PK812_06695"
     CDS             4045..4713
                     /locus_tag="PK812_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HRJ69280.1"
                     /translation="MPSNVHYKGPEDMPEVIPVFPLAGALLLPRGHIPLNIFEPRYLA
                     MVDHALAGHRLIGLIQPESDADTATAPPLYRVGCVGRLTEVAETGDGRYIITLTGVAR
                     FQIVEEQAVLTPFRQCRVDFLPYARDFVPRLGEAEVDRTALLNALRAFSEAKSIEIDW
                     DGVSEAPNEALVNALAMMSPYGVREKQALLEAPDLKSRSELLVAITEIELSRDSGDTD
                     ITVQ"
     gene            4725..4931
                     /locus_tag="PK812_06700"
     CDS             4725..4931
                     /locus_tag="PK812_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018328586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HRJ69281.1"
                     /translation="MSAETTDRTAPRAIDPKFLELLVCPLTKGLLTYDAVHQELVSES
                     AKLAYPIRDGIPILLVDEARRLEG"
     gene            complement(5067..6284)
                     /locus_tag="PK812_06705"
     CDS             complement(5067..6284)
                     /locus_tag="PK812_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018261306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HRJ69282.1"
                     /translation="MSERIDLIIAGGGAAGLSLALAVHVASDGALTVAVCDPALSASH
                     RGDSRAYALVAGARRFLDSLGAWQGMAAAAEPITGMDITDSGLNEPVRPLILNFGGER
                     PDGEPFAHMVPNGAILAALTRRAQEVGIRLVGRPVEAVERSPRRVRVRADACQFDAPL
                     LVAADGKASALRQQAGIPFYGWDYGQTAIVGTISHSLPHQGRAVEHFLPAGPFAMLPL
                     PGNACSLVWTERTVDARRFLAMPQEDLIDQIDRRAAGRFGVITAVRDIGGFPLSLGIA
                     RSFVAERLALLGDAAHVMHPIAGQGLNYGLRGAAALAETIVDSARVGLDIGSPVALEP
                     YERSRRSDVVRMALATDALNRLFSNDAGPLRLLRDVGLGLVERAPRQKDWFIAEAAGE
                     TAAAPRAFRGLAI"
     gene            6590..6928
                     /locus_tag="PK812_06710"
     CDS             6590..6928
                     /locus_tag="PK812_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HRJ69283.1"
                     /translation="MKTVMAIIKPFKLEEVRDALTAIGVHGMTVTEVKGYGRQKGHTE
                     IYRGAEYAVSFLPKLKLEVAVESAQVAAVIDAIASAARTGQIGDGKIFVSPLEQAVRI
                     RTGERDADAL"
     gene            6954..8264
                     /locus_tag="PK812_06715"
     CDS             6954..8264
                     /locus_tag="PK812_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HRJ69284.1"
                     /translation="MTVSKTSARLVAFLTATPALAQTPAKIDAADTGFMIVSTALVLM
                     MTLPGLALFYAGMVRRKTVLATMAQSAAALAMVSVLWAIAGYSLAFSGDGAWLGNLDR
                     LLLGGMAFDGVSSLAPTIPEALFMLYQMTFAVITVALIGGAVADRMRFSAFLLFGALW
                     LFLVYVPSAHWVWGGGFLAKMGVLDFAGGTVVHINAGVAGLVAALVLGPRHGYGRDNL
                     APYDLSMAVVGTGLLWVGWFGFNGGSALGAGPRAVYAITATHLAACSGALVWAALEWI
                     QRRKPSVLGIISGAIAGLGTVTPASGFILPWHGLVIGLIAGAVCYWACILLKPKAGYD
                     DSLDVFGVHGVGGIIGTLLAGVFATGIVSASADAPQGLNGLLAGNPGQVATQAIGVAV
                     IILWCAIGTFVALKITALFTPLRVGKDEEIEGLDLALHGERLHQ"
     gene            8506..8844
                     /locus_tag="PK812_06720"
     CDS             8506..8844
                     /locus_tag="PK812_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HRJ69285.1"
                     /translation="MKTVMAIIKPFKLEEVRDALTAIGVHGMTVTEVKGYGRQKGHTE
                     IYRGAEYAVSFLPKLKLEVAVESAQVAAVIDAIASAARTGQIGDGKIFVSPLEQAVRI
                     RTGERDADAL"
     gene            8936..10348
                     /locus_tag="PK812_06725"
     CDS             8936..10348
                     /locus_tag="PK812_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004676804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HRJ69286.1"
                     /translation="MPALAQATAGPAPAKGDIAWMIVATLLVLMMSVPGLALFYGGLV
                     RSKNMLSVLTQVFAIVCLSGLLWVVIGYSLAFSPGGALNDYIGGLSKAFLSGVDVNAT
                     AATFSNGVVLPELLFMAFQMTFAMITPALIVGAFAERIKFSALILFITLWLVLVYYPI
                     AHMVWYWAGPDAIGDAAKALAEAKDAAGKAAAQARLDAALADAGMIYKWGALDFAGGT
                     VVHINAGIAGLVGCLMIGKRVGYGRELLAPHSLTMTMIGASLLWVGWFGFNAGSALEA
                     NGTAVLAFVNTMVATCAAALSWLFVEWAMKGKPSLLGMVSGAVAGLVAVTPASGLAGP
                     MGAIVLGLASGAVCFAFCSYVKNAFGYDDSLDVFGVHCIGGILGAIATGFLVAPGLGG
                     TGLTDYVMKPGQGAPGDYVMATQVIAQLKAVVVTLLWSGLVSAALFKLVDTLVGLRVT
                     VEKEREGLDIAEHGERAYTA"
     gene            10511..>10821
                     /locus_tag="PK812_06730"
     CDS             10511..>10821
                     /locus_tag="PK812_06730"
                     /inference="COORDINATES: protein motif:HMM:NF024882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HRJ69287.1"
                     /translation="MRTTRLGSSPADYIPEALRDAVSRRLTEAAGLGLLALAGAGALA
                     LASWSVRDPSFSHAIDGTAKNLLGFPGAAVADLLMQVVGLASVALLLVIAIIGWRLMA
                     R"
BASE COUNT         1779 a         3669 c         3516 g         1857 t
ORIGIN      
        1 gaggcccatg gccttgcgct cgtggaattc accgcccgct cgcgcgaagc cccgacggcg
       61 gagaccatcc gcgccgcgcg cgcgatgttc gacaccctcg cctatccggc ggtgctgcat
      121 tgcaaatccg gcgccgaccg cgccggcttc atggcagcgc tctatctcat cctgcgggag
      181 aacgcgcgcg tcgacgaggc gctcggccag ctctccgccc gttacgggca ctggcgctgg
      241 agcaagaccg gcattctcga tgccttcctc gaacgctacc gcgacgaagg cgagtcgaag
      301 ggcatcggcc tgatcgactg ggcgacgcac cactacgatc cggcgcggct gacagccgaa
      361 ttccgcccga gcttctggtc gaatttcgtg gtggaccgga ttctcaggcg cgagtgagcg
      421 cgccgcccgc gtcgccgaac tgtagcctgt ggaagcccgc gtaagcgcca tccgccttca
      481 gcagggactc gtgcgtgccg gattcgacca cgcgaccggc ctccatcacc acgatcaggt
      541 ccgccgcgcg cacggtcgcg agccggtgcg ccaccaccag cgtggtgcgc ccgcgcatca
      601 gatcggccag cgctttctgc accagatcct ccgactgcgc atcgagcgcg ctcgtcggct
      661 cgtccagaag caggatcggc gcgtcgcgca ggaaggcgcg agccagcgcg atgcgctggc
      721 gctcgccgcc cgaaagacgc ccgccgcgat cgccgaccat cgtatcgtaa ccttgcggca
      781 gggcgaggat gaagtcgtgc gcggcggcgg ccctggccgc tgcaatcacc tcgtcctcgc
      841 gcgcgccggg ccggccgaac aggatgttgg cgcgcaccgt gtcgtcaaac agcacgatgt
      901 cttgggaaac cgtggcaatc ttggcccgaa gactcgccag cgtcacgtcg cggatgtcgg
      961 tcccgtcgat cagcacgcgc ccgccggtgg ggtcgaacag gcgcggcgcg agcgccagca
     1021 ggctggactt gccggcaccg gaacggccga ctatggccgt ggtcttgccg gccggagcaa
     1081 caaaatcgac gccattcagg gcaaggcccg atccggcata ggcaaagctc accgcatcga
     1141 agcgaacctc cccacgggat acctccagcg ctccggcacc tggctgttcg ctgacgcccg
     1201 gcggctcatc gatcaccgcg aacaggcggg tgagcgccgc cagcgcttcc tgaacgatgg
     1261 cattgaggtt accgagcgag cgcagcgact gggccgccag caggatggcc gtgacgtatc
     1321 cggtaaagtc gccgatggtc gactggccgc gcacgatgcg ccagccgatg aagatcagca
     1381 cgccggacac ggccaggccg ccggcgatct ccagcaacgg atccatgcgc gcgcgctggt
     1441 tcgccgcctt caccttgagg gcgcggaccg tgtcgaaggc ggtcgcggcc ttgtccttga
     1501 gataggcctc gagcccgtaa gtcttggcca cgcgggcacc cgagaggctc tcggccacca
     1561 tcgaggccat ctggccggtc tgttcctgcg tggagagcgc ggtgcggcgc agtttcttgc
     1621 cgatcttgtt gagcggtggc accacgatcg gcagcacgac cagggcgcag agcgtcacct
     1681 gccagtcgat ccagaacatc acgccgagca cggcgacgcc cgtggtcacg tcgcgcagga
     1741 acacggtgga gatgcgtgtc agcgcctcgc ggataaaggc gaaatccgtg gtgaagcgct
     1801 gggtcagcgc cgccggcgtc tcacgcgaca attgtgcgat gtcggcgtcg atcagatgct
     1861 tgtagagcgt cgtttgcagg tccgcttcga cctgcgacac cacgcggttg gtcagcgtgg
     1921 tctgggcata catcgccatg ccgcgcacgc tggtgaccag aatgacgaag agcggggtcc
     1981 actgcacgag ccgcttgttc tgcgtatcgt aggcgtcgaa gatcgtcttg atcaggtagg
     2041 gataaagcgc cgacatgatc gcaatggtgc cgatcgcggc ggcaatggcg agcagcgtcc
     2101 agcgatgcgg gcgcacccat cccgcccaca gccggcgcat gatcggccaa aggtcggagt
     2161 gggcaaggac attgctcttt ccggccggcc tgggggcagt cggagccggc gcaggggacg
     2221 cgctcatgca cgcctgcgca gcgaagggcg atgaatttgc gcttgctgaa atgccacgtc
     2281 cgccagaccg tcggtaagcc gcccttacgg cggagaaact ggagcgggcg aagggattcg
     2341 aaccctcgac cccaaccttg gcaaggttgt gctctacccc tgagctacac ccgcatccat
     2401 gaccgcgtcg ccgcagccga ggccggtcct atgcatcatt tggctcaggc gcgcaagccc
     2461 gttatcaggg atcgtgtcaa ttccggaaat cacgatgaac gatcacgaaa cgaccgatat
     2521 ttccgccccg ttgctcgcct ttctcgaccg cttgggcatt gaaacgcgga ccatggatca
     2581 tgcccccgtc ttcacggtcg ccgaatcgca ggccctgcgc ggcgccatag ccggcgccca
     2641 ttccaagaac ctgttcgtca aggacaagaa aggtcgcctg ttcctggtga ccgcgctgga
     2701 ggacgccagc ctcgacctga aaagactgca cgaggcgatc ggcgcacagg gccgcgtgtc
     2761 ctttgccagc gcggaccagt tgcgggagca cctcggcatc gaaccgggtt cggtgacccc
     2821 cttcgccgtc atgaacgacc gggccggcgc cgtgaccatg gtcctgcagg aggccctgat
     2881 gcgccacgac gtgcagaatt tccatcccct caccaaccgg cgcaccacga ccatccggcg
     2941 cgcagatctc gtgcggtttc tcgaggccgc gaatcacccg ccgcggatcg ttgcccttcc
     3001 cgaggccgcg aaataaacct gaagctccga cgcggcacgt tgcgaaacgg cggcgcacgc
     3061 gccatctaga ggtcgaaatc aggagcgggc ccggtcacgg gccctcgcgg gctgggtgat
     3121 acagacgatg atgcaggcag gcggcacagc aacggatgct ttgatccgtg aaaccacgac
     3181 ggcggcgttt cgccaggatg tgatcgccga atcggcgaag cagccggttc tcgtcgattt
     3241 ctgggccccc tggtgcggcc cttgccgcca gttgacgccc gtcatcgaaa aagtggtgaa
     3301 ggccgccggc ggcaaggtga aactggtcaa gatgaacatc gatgaccatc cccaggtagc
     3361 cggtcagatg ggaatccagt caatcccggc cgtcatcgcc ttttcgaagg gacagccggt
     3421 cgacggcttc atgggcgccc tgcccgaaag ccaggtcaag actttcatcg agcgtcttgt
     3481 cggccctctt ggggcgggcg gcacggccga actgctgaag caagcggcgg cggcggccga
     3541 atcgggcgac catcccggcg cggccgaact gttcgccgcc gtgctggcgc aggagccgga
     3601 aaacctccag gcgctgggcg ggctggcgaa ggtgcagctt gacgccggca gtcccgagca
     3661 ggcgcgccgc ctgatcgaca ccgtgccccc ggcgaagcac gccgaccctg ccatcgcctc
     3721 cgtgctcgcc gcgctggaac tggccgaacg cgccgccagc cttggcgatc tggtcgacct
     3781 cgaacgccgc atcgccgtcg atccggccga ccatcaggcc cggttcgatc tcgcggttgc
     3841 gctcaacagc aagggcttca aggcggaggc ggccgaccac ctgctggaga tcatccggcg
     3901 cgaccgctcc tggaacgagg acggtgcgcg caagcagctg ctgcaactgt ttgaagcctg
     3961 gggaccgatg gacgaggtga ccatcggcgc acgccgtaag ctgtcggcct acctgttccg
     4021 ctgaacccgt cgggaagcat cacgatgccg agcaatgtcc actacaaggg acccgaggac
     4081 atgccggagg tcattccggt tttcccgctt gccggcgcct tgctgctgcc ccgtggccac
     4141 atcccgctca acatttttga gccgcgctat cttgccatgg tggaccacgc cctggccgga
     4201 catcggttga tcggcctgat ccagccggaa tcggatgccg acacagcgac ggctccacca
     4261 ctctatcgcg tcgggtgcgt tggccgcctg accgaagtcg ccgagaccgg cgacggtcgc
     4321 tacatcatca ccctgacggg tgtcgcccgg ttccagatcg tcgaggaaca ggccgttttg
     4381 acgccgttcc gccagtgtcg tgtcgacttc ctgccctatg cccgggactt cgtgccgcgc
     4441 cttggcgaag ccgaggtgga ccgcaccgcg ctgctcaacg ccctgcgcgc cttttccgaa
     4501 gcgaaatcga tcgagatcga ttgggatgga gtgtccgagg ccccgaacga ggcgctcgtc
     4561 aacgcgctgg cgatgatgag cccctacggg gtacgggaaa agcaggccct gctggaagcc
     4621 cccgacctga agtcccgctc cgaacttctc gtagccatca ccgagattga actgtcccgt
     4681 gattcgggcg atacggacat cactgtccag taggagttcg ggccatgtcc gccgagacca
     4741 cagaccgcac cgcgccgcgc gccatcgatc ccaaattcct cgaactgctg gtctgcccgc
     4801 tgacgaaggg gctgttgacc tacgacgccg tgcaccagga actcgtgagc gagagcgcca
     4861 agctcgccta tccgatccgc gacggcattc cgatcctcct ggtcgacgag gcccgtcgcc
     4921 tcgagggctg aggccggcaa cgcgcgccgg aaagcccgcc tgatcggcgc aaccgggtat
     4981 gtccatgccc gccgtggcag ctagcatcgg ccgactgccc ggtaggaaac tgtgccgatg
     5041 ccaccgtcgc ggtgacacca ggacgatcag atcgccagcc cacggaaagc ccgtggcgcc
     5101 gccgcggtct ctcccgcagc ctcggctatg aaccagtctt tctggcgcgg cgcgcgctca
     5161 accaggccga ggccgacatc tcgcaacaaa cgcagcgggc cggcatcatt ggaaaacagc
     5221 cggttgagcg catccgtggc cagcgccatg cgcaccacat cggagcgccg ggagcgttcg
     5281 taaggttcga gcgcgaccgg agacccgatg tctaggccga cacgcgcgga gtcgacgatg
     5341 gtttcggcca gagccgccgc gccacgcaac ccgtagttca ggccctggcc cgcgatcgga
     5401 tgcatgacat gcgccgcatc ccccagcagc gccagccgtt ccgccacgaa actgcgggcg
     5461 atgccgaggc tcagcggaaa accaccgatg tcacgcacgg ccgtgatcac gccaaaccgt
     5521 ccggccgcgc gccggtcgat ctgatcgatc agatcctcct gcggcatcgc caggaaccgt
     5581 ctcgcgtcca ccgtgcgttc ggtccagacc agcgagcacg cgttgcccgg cagcggcagc
     5641 atggcgaagg gaccggcggg caggaaatgc tccacggccc gtccctggtg cggcagcgaa
     5701 tgactgatcg tgccgacgat ggcggtctgc ccgtaatccc acccgtagaa cggaataccc
     5761 gcctgctggc gcagcgccga ggccttcccg tcggcggcga ccagcaacgg cgcgtcgaat
     5821 tggcatgcat ccgcgcgaac cctgacgcgg cgtgggctgc gttcgacagc ctcgaccggt
     5881 cggcccacca gccggatgcc gacttcctgg gcgcgtcggg tcagcgccgc gaggattgcg
     5941 ccgttgggaa ccatgtgggc gaacggctcg ccgtccggcc gttcgccgcc gaagttcagg
     6001 atcagcggcc gcaccggttc gttgaggccg ctgtcggtaa tgtccatgcc ggtgatgggt
     6061 tcggccgctg cggccatgcc ctgccaggct ccgaggctgt cgagaaaccg ccgcgcgccg
     6121 gcgaccagag cataggcgcg ggaatcgcca cggtggctgg cggacagcgc cggatcgcag
     6181 accgcaaccg tgagcgcccc gtcggacgcc acatgcaccg caagagccag cgaaagaccc
     6241 gccgctccgc cgcctgcgat gatcaggtcg atcctctcgc tcatgtccac tccgcttgcg
     6301 cactgccgtt cgacggccac catatcgcaa tcctgcggtt tggagcagcc tccaggcggc
     6361 gcgaccgcct gccgcagaga ctgcacgccg cttaggcgtc gcgcccaaga attcggcaga
     6421 ttggcgcgca ggacgcccag atcgccggca gattcgcggc cgggcaggac actctcggcg
     6481 ccattgcggt gcttttgcct ttggcatgcc gcttgtttgc tcgtcacccc gccgagggtt
     6541 tgggcgcgaa atccccggtc cgttccggtt cttgcgcgac gagggttcca tgaagaccgt
     6601 gatggcgatc atcaagccgt tcaagctgga ggaggtgcgc gacgcgctca cagccatcgg
     6661 cgtgcacggc atgaccgtga cagaggtgaa gggctacggc cggcagaaag gccataccga
     6721 gatctatcgc ggcgcggaat acgccgtgag ttttctgccc aagctgaagc tcgaggtggc
     6781 ggtggaatcc gcgcaggtgg ccgcggtgat cgatgcgatc gcctcggcgg cacgcaccgg
     6841 acagatcggc gacggcaaga tcttcgtctc cccgctcgag caggccgtcc gcatccgcac
     6901 aggcgaacgc gacgccgatg ccctctgatc ccaattctga ttcaggcaaa gccatgaccg
     6961 tttcgaaaac ttccgcgcgc ctcgtcgcgt tcttgaccgc aacgccggcg ctcgcccaga
     7021 cgccggccaa gatcgacgcc gcagacaccg gcttcatgat cgtgtcgacc gcccttgtgc
     7081 tgatgatgac gctgccgggc ctcgcactgt tctacgccgg aatggtgcgc cgcaagaccg
     7141 tccttgcgac catggcccag tcggccgccg cgctcgcgat ggtttcggtc ctgtgggcaa
     7201 tcgccggcta ttcgctggcc ttttccggcg acggcgcctg gctgggaaac cttgatcggc
     7261 tgctgcttgg cggcatggcg ttcgacggcg tctcgagcct cgccccgacc attcccgaag
     7321 ccctgttcat gctctaccag atgacgttcg cggtcatcac cgtcgcgctg atcggcggcg
     7381 cggtcgcgga ccgcatgcgc ttttcggcat tcctcctctt cggcgctctg tggcttttcc
     7441 tcgtctacgt acccagcgcc cactgggtct ggggcggcgg attcctcgcc aagatgggcg
     7501 tgctggattt cgccggcggc acggtcgtgc acatcaacgc cggcgttgcc ggccttgtcg
     7561 ccgccctcgt gctgggtccg cgacatggct atggccgcga caaccttgcg ccctacgatc
     7621 tgtcgatggc ggtggtgggc accggcctgc tctgggtcgg ctggtttggc ttcaacggtg
     7681 gttcggcgct cggcgcaggc ccacgcgccg tctacgccat caccgccacg catcttgcgg
     7741 cctgttcggg cgcgctggtc tgggctgcgc tcgaatggat ccagcggcgc aagccttccg
     7801 tcctcggcat catctccggc gccatcgccg ggctcggcac cgtcaccccg gcctcgggct
     7861 tcatcctgcc ctggcacggg cttgtcatcg gcctgattgc cggcgccgtc tgctactggg
     7921 cctgcatcct gctcaagccg aaggcaggct acgatgattc gctggacgtg ttcggcgtgc
     7981 atggtgttgg cggcatcatc ggcacgctgc tggcgggtgt tttcgccacg ggtatcgtct
     8041 ccgcttccgc cgacgcgccg cagggtctga acggcttgct cgccggcaat cccgggcaag
     8101 tcgcgacgca ggcaatcgga gtcgcggtca tcatactctg gtgcgccatc ggcaccttcg
     8161 tggcgctcaa gatcaccgcc ctgttcacgc ccctgcgcgt cggcaaggac gaggagatcg
     8221 agggacttga tctggcgctt cacggcgagc gcctgcatca gtaatccaaa ggaccgcgcg
     8281 caccggtgac ccggcagccc ccgctgccgg gttttttgcg ttgcaacagc aagcggcgac
     8341 gccctgccca agaaaacagc aggacgccgg aacaacgggc aactcgaggc cagccgcgct
     8401 gcgacttcgc cagaatccgg ccttggcacg cgacttgtta ccgacctcct gacccggccg
     8461 ccgccgcggc ggctgcgcaa caggcgtcta aggagagcga taccgatgaa gaccgtgatg
     8521 gcgatcatca agccgttcaa gctggaggag gtgcgcgacg cgctcacagc catcggcgtg
     8581 cacggcatga ccgtgacaga ggtgaagggc tacggccggc agaaaggcca taccgagatc
     8641 tatcgcggcg cggaatacgc cgtgagtttt ctgcccaagc tgaagctcga ggtggcggtg
     8701 gaatccgcgc aggtggccgc ggtgatcgat gcgatcgcct cggcggcacg caccggacag
     8761 atcggcgacg gcaagatctt cgtctccccg ctcgagcagg ccgtccgcat ccgcacaggc
     8821 gaacgcgacg ccgatgccct ctgaatgccc ccaatcttcc ggagctgaac ccatgacctt
     8881 tttgtcgaac ctgcggcggc caacagccgg ccttgcccta tgcctgttgt ccgcgatgcc
     8941 cgccctggcc caggcaaccg ccgggcctgc gccggcaaag ggcgatattg cctggatgat
     9001 cgtcgccact ctcctggtcc tgatgatgtc ggttcccggc ctcgcgctgt tctacggtgg
     9061 cctggtgcgt tccaagaaca tgctgtccgt gctgacccag gtcttcgcca tcgtctgcct
     9121 gtccgggctg ctctgggtgg tcatcggcta ttccctggcc ttttcacccg gcggcgcgct
     9181 gaacgactac atcggcggtc tgtcgaaggc gttcctgagc ggtgtcgacg tcaacgccac
     9241 cgccgcgacc ttttcgaacg gcgtcgtcct tccagagctg ctcttcatgg ccttccagat
     9301 gacgttcgcc atgatcacgc cggcgctgat cgttggcgcc ttcgccgaac gcatcaagtt
     9361 ttcggcgctt atcctgttca tcacgctttg gctggttctt gtctactatc cgatcgccca
     9421 catggtctgg tactgggccg gacccgacgc catcggcgat gcggccaagg ccctggctga
     9481 agccaaggat gcggccggca aggcagccgc tcaggccagg cttgacgccg cgcttgccga
     9541 tgccggcatg atctacaagt ggggcgcgct ggacttcgcc ggcggcaccg tggtccacat
     9601 caatgcgggc atcgccgggc ttgtcggctg cctgatgatt ggcaagcgcg tcggctacgg
     9661 gcgcgaactg ctggctcccc attccctgac gatgaccatg atcggcgcgt cgctgctctg
     9721 ggtgggctgg ttcggcttta acgccgggtc ggcgctggag gcgaacggaa ccgccgtgct
     9781 ggcttttgtc aacaccatgg tggcgacctg cgcggcagcc ctgtcgtggc tgttcgtcga
     9841 atgggcgatg aagggcaagc cctcgcttct gggcatggtt tcgggtgccg tggcaggcct
     9901 tgtcgccgta accccggcct ccggtcttgc cggtccgatg ggagccatcg tcctgggcct
     9961 tgcctctgga gcggtatgct ttgcgttctg ctcctacgtg aagaatgctt tcggctacga
    10021 cgattccctc gacgtgttcg gcgtgcattg catcggcggc atactggggg ccatcgccac
    10081 gggcttcctg gtggcgccgg gcctgggcgg cacaggcctg accgactacg tcatgaagcc
    10141 agggcagggc gcacccggcg actatgtgat ggcgacacag gtcattgccc agcttaaggc
    10201 cgtggtcgtg accctgttgt ggtccggcct ggtttcggcc gccctcttca agcttgtcga
    10261 cacgctggtt ggcctgcgcg tgactgttga aaaggagcgc gagggcctcg acatcgccga
    10321 acatggcgag cgtgcctaca cggcctgacc gacaccggaa cgcaacgaca gggcggcggg
    10381 tcccatcggg gcccgccgcc ggcgtttggt cccgactgcg cgccgcgatt gcgcgcatcc
    10441 ttaaggcgga cttaaccgct tctgaaccat gatcggccgg ttttctggaa acgggttgcg
    10501 gaacgggttc atgcgtacga cgcgtttggg gtcttcgcct gcggactaca ttccggaggc
    10561 gctacgcgat gctgtctccc gccgcctcac cgaggcggcc ggtcttggcc tgttggcact
    10621 cgccggggca ggagcgctgg cgctcgcgag ctggtccgtg cgcgatccga gtttcagcca
    10681 cgcaatcgac ggtacggcga agaatcttct cggctttccg ggagcggccg tggccgacct
    10741 gctcatgcag gtcgtcggcc tggcttccgt cgcgctgttg ctggtgatcg ccatcatcgg
    10801 ctggcggctg atggcgcgga g
//
LOCUS       DAOMXN010000068        10783 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_3266_length_10783_cov_11.882177, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000068 DAOMXN010000000
VERSION     DAOMXN010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 10783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10783
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3266_length_10783_cov_11.882177"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..1077
                     /locus_tag="PK812_06735"
     CDS             196..1077
                     /locus_tag="PK812_06735"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRJ69288.1"
                     /translation="MPSPFIVAALGMLVLTILDSFIKSLAGTYATFQIVCLRFAFGAL
                     WASAIVAYSGHSLPPAARMKAHMARAALMIVTASGFFYALGKVPLAELFAISFTAPIF
                     TAIFGAVFLKEKVLPSIMIAIAAGFAGMLVIVLGPTEGGGLRLAYEPFALFCAVISPI
                     TYALAIVLLRSQASSEPMSVIISVQSAMVALAVSPLAIATFEPPTVVDWAKFAIAGLL
                     GTTGYFAFTHALKHLTAARFSVVEYTGLIWASLIGYFIFNEVPKSTVWAGAALIIAGC
                     LLAVRAKSEGKALPEAS"
     gene            complement(1087..1461)
                     /locus_tag="PK812_06740"
     CDS             complement(1087..1461)
                     /locus_tag="PK812_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HRJ69289.1"
                     /translation="MDEIVTSPGQPAASGSAALSPEDIDALTESIVAALKTVYDPEIP
                     SDIYELGLIYRIDIADDRQVTIDMTLTAPGCPVAGEMPGWVENAVAAVPGVQGVKVNM
                     VFDPPWDQSRMSDIARVALDMW"
     gene            complement(1473..2696)
                     /locus_tag="PK812_06745"
     CDS             complement(1473..2696)
                     /locus_tag="PK812_06745"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HRJ69290.1"
                     /translation="MTYDIARIRADFPALSMQVYGKPLVYLDNAASAQKPRQVLDRVR
                     QAYESEYANVHRGLHYLANAATEAYEAARDKLRAFLNAPSTDEIIFTKNATEALNLIA
                     SSLGRALKIGEGDEIVLSIMEHHSNIVPWNYWRENAGAVIKWAPVDADGNFLLEDFEK
                     LLTPKTKVVAITQMSNALGTIVPVKEVVALAKARGIATVVDGSQAAVHLDVDVQDIGC
                     DFYVITGHKLYGPSGIGALFGRREWLERMPPFLGGGEMIREVSEEGVTYGAPPHRFEA
                     GTPPLVQAIGLGAALDYITAIGKPAIREHEDALVRYAHERLGAINALTIYGKARQKGA
                     IVSFALEGAHPHDIATVIDRHGVAVRAGTHCTMPLLRRYGVTATCRASFALYNTREEV
                     DKLAEALTRAQAMFS"
     gene            complement(2693..4015)
                     /gene="sufD"
                     /locus_tag="PK812_06750"
     CDS             complement(2693..4015)
                     /gene="sufD"
                     /locus_tag="PK812_06750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HRJ69291.1"
                     /translation="MSYAGEKTKAETMLAEQFAVALPGLPGNDPAMKQRRSAAFAHIL
                     RDGLPSKRNESWHYTDLRMKMPEAALPMLALLPSTIPTGKAPADPCAAATPVHFSFAS
                     GLTLEGRSVPEGLSFVSIRDGLVRNHPMVAHLGIAARDEASPIVALNSAFVTDGIILR
                     VAKGVRIAEPIRMFAPFAMEMARAATSRIVVILEEGAELSLVMSQVGPNGIAYQSNVV
                     CECQVADDAKLDIVRLNLAGDRAQVLVNTSASTGARSEFNFFAMSAGGAVSRHEIQLA
                     STGENAKVRLNGVILAKAEQHTDFTLEVDHRAPGCESRELFRSVIDGDATGVFQGKIM
                     VKREAQRTDGKMASNAILLSDGATMNNKPELEIFADDVQCAHGATCGALDEDLLFYLQ
                     SRGLPKAEAEALMIQAFCGEAIESISNEATRDVFNGLVARWLKARSCR"
     gene            complement(4023..4778)
                     /gene="sufC"
                     /locus_tag="PK812_06755"
     CDS             complement(4023..4778)
                     /gene="sufC"
                     /locus_tag="PK812_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020486090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HRJ69292.1"
                     /translation="MLEIRNLHVKIADSDKAILNGLTLTVKAGEVAAIMGPNGSGKST
                     LSYVVSGKEDYEITEGDILLNGQSILDMEAHERAAAGLFLAFQYPLEIPGVATMTFLK
                     AAMNAQRKSRGEAEISPTDFMKAVRGAADKLGINQDMLKRALNVGFSGGEKKRMEILQ
                     MALLQPKFCILDETDSGLDIDALRIVSEGVNALRAPDRGMLVITHYQRLLDHIVPDTV
                     HVMAKGRIVKSGGPELAHDLEKNGYAAYVGEAA"
     gene            complement(4812..6281)
                     /gene="sufB"
                     /locus_tag="PK812_06760"
     CDS             complement(4812..6281)
                     /gene="sufB"
                     /locus_tag="PK812_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011975623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HRJ69293.1"
                     /translation="MAAVKETVDHVRSIDVDQYKYGFVTDIESELAPKGLNEDIVRYI
                     SAKKGEPQWLLDWRLDAFRRWKTMTEPSWARVSYPPVDFQDLHYYAAPKKTPGPKSLD
                     EVDPELLATYEKLGIPLREREVLAGVEGAKVAVDAVFDSVSVVTTFKEELAKAGVIFC
                     SISEAVKEHPDLVRKYLGTVVPTTDNFYATLNSAVFSDGSFVYIPPGVRCPMELSTYF
                     RINEQKTGQFERTLIIAAEGAYVSYLEGCTAPKRDENQLHAAVVELIALDEAEIKYST
                     VQNWYPGDKDGKGGIYNFVTKRGDCRGKKSKISWTQVETGSAITWKYPSCILRGDGSS
                     GEFYSIAISNGYQQVDSGTKMIHLGRNTTSKIISKGIAAGHSDNTYRGQVSAHRKATG
                     ARNFTNCDSLLIGDLCGAHTVPYIESKNASVVFEHEATTSKIAEDQLFYCMQRGLDEE
                     AAVALIVNGFVKDVLQQLPMEFAVEAQKLIAVSLEGSVG"
     gene            complement(6344..7489)
                     /locus_tag="PK812_06765"
     CDS             complement(6344..7489)
                     /locus_tag="PK812_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015340015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HRJ69294.1"
                     /translation="MARRRAYLDWNATAPVLPDVAEAVARALELGNPSSVHAEGRAAR
                     AAIEKARAEVAALVGAEPGQVTFTSGATEAAAMALQPVSTEETLFVGASEHPCIRAGG
                     GFDALRRHVMPVDEAGRFDFAALAEVAGGRARPDLIHVAVQAANNETGVLNDGNAIAR
                     LKAQGFTVTCDAVQAAGKLRLDARTLGADFLLLSAHKIGGPKGVGALIARADACGGVR
                     PLLRGGGQERGLRAGTENVAGIVGFGVAARLAQARLPEMERVRTLRDRFERDLRSLAP
                     DVVVFGAQATRLPNTSLFAIPGLRAETAVIAFDLDGVAVSSGSACSSGKVGRNAVLDA
                     MGISSALADGAIRVSLGWTSDEDDINQALDSVRRQLTRVAPRRQSAA"
     gene            complement(7585..8187)
                     /locus_tag="PK812_06770"
     CDS             complement(7585..8187)
                     /locus_tag="PK812_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69295.1"
                     /translation="MLIGVVGWLLYRHGPRWSFLLAPVLVLGPPLADRVHSTGEFHKA
                     CHAAASGQVVKRVQADGFLLESAHAGDFGTSHLLEGFRWIERRSRSGGWERVSANPDG
                     SVSVQTIDEPTARYMVVETTDQARSWKRFTRRIVDRQDATTLATSGYAVYLGGTVTWM
                     LAPFDHDDCAWVPGGSRFNQYFQLTKYVLQPSAPEAGPKP"
     gene            8408..9064
                     /locus_tag="PK812_06775"
     CDS             8408..9064
                     /locus_tag="PK812_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017484188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRJ69296.1"
                     /translation="MPEIIFNGPAGRLEGRYQPAKQRGAPIAIILHPHPQFGGTMNNQ
                     IVYNLYYTFADRGFSVMRFNFRGVGRSQGVFDHGQGELSDAAAALDWAQALNPDAKTC
                     WIAGISFGAWIGMQLLMRRPEIEGFISIAAMANRYDFSFLAPCPSSGLFVHGDQDRVA
                     PYNEVISVIDKVKTQKGVKIEHTLIKGANHFFENHVEELTGVVGTYLDKRLGTKGQAA
                     "
     gene            9069..9677
                     /locus_tag="PK812_06780"
     CDS             9069..9677
                     /locus_tag="PK812_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019170577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE/ArgO family amino acid transporter"
                     /protein_id="HRJ69297.1"
                     /translation="MLSAAIAGFLLGGSLIVAIGAQNAFVLRQGLLRHNVFAVTLFCA
                     VSDAVLIALGVAGFGAYVASSPGLLRIVGIAGAAFLFAYGGMAFRRALQPSVLTATEA
                     PAPPLATALASCASFTFLNPHVYLDTVVLVGGLAATWPGSSRYVFGAGAILASFVWFF
                     GLGYGARLLEPVFRSPRAWQVLDCLIGLIMWMLAARIALPLF"
     gene            9738..>10783
                     /gene="tyrS"
                     /locus_tag="PK812_06785"
     CDS             9738..>10783
                     /gene="tyrS"
                     /locus_tag="PK812_06785"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRJ69298.1"
                     /translation="MTTYKSDFLATLSERGFIHQCSDFEGLDAKAAKGEVVAYVGYDC
                     TAPSLHIGNFLSLMMLYWLQQSGNKPITLMGGGTTRIGDPSGKDEARALRSIEEINSN
                     KAAIRGVFAKILSFGDGRTDAVMIDNAEWLPELKWIDMLRDVGRHFSVNRMLTMDSVR
                     LRLEREHEMSFIEFNYMIAQSYDFVELSKRLGCNLQMGGSDQWGNIIMGVDLGRRMGT
                     HQLYALTTPLLTTASGAKMGKTAQGAVWLNAEQFSPYDFWQYWRNTEDADVGRFLKLF
                     TTLPMADIRKLEALGGAEINEAKKALATAATTLLHGAAAAEQAAETARITFEQGALAA
                     DLPSVGVPKAELAAG"
BASE COUNT         1818 a         3581 c         3374 g         2010 t
ORIGIN      
        1 cctgctgcgg gacgcggaaa gccggggcgc ccggacgctg gaatgcttcg ccgcgaaccg
       61 cgccgccatc cgcttctacc agcgcgaagg ctggacaatc acagcggcct acgagcgcag
      121 tttcgccgga gcgccgcatt ccttcgtgcg ttttgcgcgc ccgccgctat tgtaggcgcg
      181 attgaggaac ccaaaatgcc gtcgccgttc atagtcgccg ccctcggcat gctcgtcctg
      241 accatccttg attccttcat caagtccctg gccggtacct acgcgacgtt ccagatcgtc
      301 tgcctgcgat ttgccttcgg cgcgctctgg gccagcgcca tcgtggccta cagcggtcat
      361 tctctgcccc cggccgcgcg gatgaaggcg cacatggccc gcgccgccct gatgatcgtg
      421 acggccagtg gcttcttcta tgccctcggc aaggtaccgc tggccgagct cttcgccatt
      481 tccttcaccg cgccgatctt caccgccatc tttggcgcgg tctttctcaa ggaaaaggtg
      541 ctgccaagca tcatgatcgc catcgcggcg gggttcgctg gcatgctggt gatcgtgctg
      601 ggaccgaccg aggggggcgg cttgcgcctt gcctacgagc cgtttgcact gttctgcgcc
      661 gtgatctctc cgatcaccta cgcgctggcg attgtcctgc tgcggtcgca ggccagcagc
      721 gagccgatga gcgtgatcat ctcggttcaa tccgcgatgg tggcgctcgc cgtatcgccg
      781 ctcgccatcg cgacattcga gccgccgacc gtggtcgact gggcgaagtt cgccatcgcc
      841 ggcctgctcg gcaccacagg ctacttcgcc ttcacccacg ctctgaagca cctgacggct
      901 gcgcgctttt cggtggtcga gtacaccggc ctcatctggg cctcgctgat cggctacttc
      961 attttcaacg aagtgccgaa gtcgacagtc tgggccggcg cggcgctgat catcgcggga
     1021 tgcctgttgg cggtgcgggc gaagtccgaa ggcaaggcgc tcccggaagc gagttgatcc
     1081 cgtgcatcac cacatgtcga gcgcgacacg cgcgatgtcg gacatgcggc tctggtccca
     1141 cggcggatcg aacaccatgt tgaccttcac accctgcacg ccgggaacag cggcgaccgc
     1201 gttctcgacc cagcccggca tttcgccggc gacgggacag cccggcgcgg tgagcgtcat
     1261 gtcgatggtc acctgccggt cgtcggcgat gtcgatgcga tagatcaggc cgagttcata
     1321 gatgtcggag ggaatctccg gatcgtaaac ggttttcagc gcggcgacga tgctctcggt
     1381 gagcgcatcg atgtcttccg gcgacagggc agcgctgccg ctggcggcgg gctgtccagg
     1441 cgatgtcacg atctcatcca tcggcatatc cgtcacgaga acatcgcctg ggcgcgcgtc
     1501 agcgcttcgg caagcttatc gacttcctca cgcgtgttat agagcgcaaa tgaggcgcgg
     1561 catgtagcgg tcacgccata ccggcgcaaa agcggcatgg tgcaatgcgt gcctgcccgc
     1621 accgcgacgc catgccggtc gatcaccgtg gcaatgtcgt gcgggtgggc gccctccagc
     1681 gcaaacgaaa cgatggcccc cttctggcgg gccttgccat agatcgtcag cgcattgatg
     1741 gcgccaagcc gctcatgcgc atagcgcacc agcgcatcct cgtgctcccg gatcgccggc
     1801 ttgccgatgg ccgtgatgta atccagcgcc gcgccaaggc cgatggcctg aaccaacggc
     1861 ggcgtgccgg cctcgaagcg atgcggcggc gcgccatagg tgacgccttc ttcgctcacc
     1921 tcgcggatca tctcgccgcc gcccaggaac ggcggcatgc gctccagcca ttcccggcgt
     1981 ccgaaaaggg cgccgatgcc ggaagggcca tagagcttgt ggccggtgat gacatagaaa
     2041 tcgcagccga tgtcctgaac gtcgacatcg agatgcaccg cagcctggct gccgtcgacc
     2101 acggtggcga tgccgcgcgc cttagccagc gccacgacct ccttcacggg cacgatggtg
     2161 ccgagcgcat tcgacatctg cgtgatggcg acgacctttg tcttcggcgt cagcagcttc
     2221 tcgaagtcct cgagaaggaa attgccgtcg gcgtcgacgg gcgcccattt gatcaccgcg
     2281 ccggcattct cgcgccagta gttccagggc acgatgttgg aatggtgctc catgatcgag
     2341 agcacgatct cgtcgccctc cccgatcttc agcgcgcggc ccagagagga ggcgatgagg
     2401 ttcagcgcct cggtggcgtt cttggtgaag atgatctcgt cggtcgaggg cgcattgagg
     2461 aaggcgcgca gcttgtcccg agcggcctcg taagcctccg tcgccgcatt ggccagatag
     2521 tgcaacccgc gatgcacgtt ggcgtattcg ctctcgtagg cctgccggac gcgatcgagc
     2581 acctgcctcg gcttctgcgc cgaggctgca ttgtcgagat agaccagcgg ctttccgtag
     2641 acctgcattg acagggccgg gaaatccgcg cggatgcggg cgatgtcgta ggtcatcgac
     2701 aagagcgcgc cttcaaccaa cgcgcgacaa gaccattgaa aacatcgcgt gtcgcttcat
     2761 tggagatgga ttcgatcgct tcgccgcaga aggcctggat catcagcgcc tcggcttccg
     2821 ccttcggcag gccccgtgac tgaaggtaga acagcaagtc ctcgtcgagc gcgccgcagg
     2881 tggcgccatg agcgcactgc acgtcatcgg cgaagatttc cagttccggc ttgttgttca
     2941 tggtcgcccc gtccgacaac aggattgcgt tggaagccat cttcccatcg gtgcgctggg
     3001 cttcacgctt gaccatgatc ttgccctgga acacgccggt cgcgtcgcca tcgatgacag
     3061 agcgaaacaa ctcccggctt tcgcaaccag gggcccgatg gtccacttcc agggtgaaat
     3121 ccgtgtgttg ctcggccttt gcgagaatca cgccgttgag ccgcactttc gcgttctcgc
     3181 cggtgcttgc caactggatc tcatggcgcg acacggcgcc accggcggac attgcgaaga
     3241 agttgaattc agagcgcgcg cccgtcgagg ctgaggtgtt gaccagcacc tgcgcccggt
     3301 cacccgccag attcaatcgc acgatgtcca gcttcgcgtc atccgcgact tggcattcgc
     3361 aaaccacgtt ggactggtag gcgatgccgt tcggtccgac ctgtgacatc accaggctca
     3421 gctctgcgcc ttcctccaag atcacgacga tccgggacgt cgcggcacgc gccatttcca
     3481 tggcgaacgg cgcgaacatc ctgatgggct cggcgatccg gacacctttg gcgaccctaa
     3541 ggatgatgcc atcggtcacg aaggcgctgt tcagcgcgac aatcggcgag gcttcgtccc
     3601 gcgccgcaat gccgagatgc gccaccatcg gatggttgcg caccaggcca tcacgaatgg
     3661 atacgaatga cagtccttcc ggcaccgagc gaccttcgag cgtcagcccg ctggcaaagg
     3721 aaaaatgcac cggcgtggcg gcggcgcatg ggtcggccgg cgccttgccg gtcggaatcg
     3781 tcgacggcag cagcgccagc atgggcaacg ccgcctccgg cattttcatc cgcaggtcgg
     3841 tatagtgcca ggattcattg cgcttcgacg gcaatccatc acgcaagata tgcgcgaagg
     3901 cggcgctccg gcgctgcttc atcgccggat cgttgcccgg caggcctggc aacgccacgg
     3961 cgaattgctc cgccagcatc gtctcggcct tggttttttc tcctgcatag ctcatgacgt
     4021 gctcacgccg cctcgccgac ataggcggcg tatccgttct tctccaggtc gtgcgccagt
     4081 tccggcccgc ccgacttcac gatgcggccc ttcgccatga catgcacggt gtcgggcacg
     4141 atgtgatcga gaaggcgctg atagtgggtg atcaccagca tgccgcgatc gggcgcgcgc
     4201 agggcgttga ccccctccga cacgatgcgc agcgcgtcga tgtcgaggcc tgaatcggtt
     4261 tcgtcgagaa tgcagaactt cggctggagc agcgccatct gaaggatttc catgcgcttc
     4321 ttctcgccgc cggagaagcc gacgttaagc gcgcgcttga gcatgtcctg gttgatgccg
     4381 agcttgtccg ccgcgccgcg caccgccttc atgaagtcgg tgggcgagat ttcggcttcc
     4441 ccccgggact tgcgctgcgc attcatcgcc gccttgagga aggtcatggt ggcgacgccc
     4501 ggaatttcca gcggatactg gaaggcgagg aacagcccgg cggcggcgcg ctcatgcgct
     4561 tccatgtcga ggatgctctg cccgttaagc aggatgtcac cctcggtgat ctcgtaatcc
     4621 tccttgccgg agacgacata ggagagcgtc gacttgccgg agccgttagg ccccatgatg
     4681 gccgcgacct cgccggcctt cacggtgagc gtcaggccgt tgaggatcgc cttgtcgctg
     4741 tcagcgatct tgacgtggag attgcgaatt tcgagcatgg gttgtccgta tcagatggtc
     4801 atcgtgttgg ttcagccgac cgagccctca aggctcacgg cgatgagttt ctgcgcctcg
     4861 accgcgaatt ccatcggcaa ttgctggagc acgtccttga cgaagccgtt gacgatgagc
     4921 gccaccgccg cctcctcatc gaggccgcgc tgcatgcagt agaacagctg gtcctcggcg
     4981 atcttcgagg tggtcgcctc gtgctcgaac acgacggagg cgttcttgct ctcgatatag
     5041 ggcacggtat gcgcgccgca gagatcgccg atgagcaggc tgtcgcagtt ggtgaagttg
     5101 cgcgcgccgg tcgccttgcg gtgggccgag acctgcccgc gataggtatt gtcggaatgt
     5161 ccggccgcga tgcccttgga gatgatcttg gacgtggtgt tccggcccag gtggatcatc
     5221 ttggtgccgc tgtcgacctg ctggtagccg ttcgagatgg caatggaata gaactcgccc
     5281 gaagagccgt cgccgcgcag gatgcaggag ggatacttcc aggtgatggc ggagcccgtt
     5341 tccacctgcg tccaggaaat cttggacttc ttgccccgac agtcgccgcg cttggtgacg
     5401 aaattgtaga tgccgccctt gccgtccttg tcgccgggat accagttctg gacggtggaa
     5461 tacttgatct ccgcctcgtc gagcgcgatc agttccacca cggcggcatg gagctggttc
     5521 tcgtcgcgct tgggcgcggt gcagccctcg agataggaga cgtaggcgcc ttccgcagcg
     5581 atgatcagcg tgcgctcgaa ctggccggtt ttctgctcat tgatgcggaa gtaggttgaa
     5641 agttccatcg ggcagcgcac gcccggcggg atgtagacga aggacccgtc ggaaaacacg
     5701 gcgctgttga gcgtcgcgta gaagttgtcg gtggtcggca ccacggtgcc gagatacttc
     5761 cgcaccaggt ccggatgctc cttcaccgcc tccgagatcg agcagaagat cacgccggcc
     5821 ttggccagtt cctccttgaa ggtggtgacg acggacacgc tgtcgaaaac cgcgtcaacc
     5881 gcaaccttcg caccctcgac gcccgccaga acctcgcgct cgcgcaaggg aatgccgagc
     5941 ttttcgtagg tcgccagcag ttcgggatcg acctcatcca gcgacttggg accgggcgtc
     6001 ttcttcggcg cggcgtagta gtgcaggtcc tgaaagtcga ccggcggata gctgacgcgc
     6061 gcccagctcg gctcggtcat ggtcttccag cggcggaagg cgtcgagccg ccagtcgagc
     6121 aaccattgcg gctcgccctt cttggccgaa atgtagcgaa cgatgtcctc gttcagccct
     6181 ttcggcgcca gttcggactc gatgtccgtg acgaaaccgt acttgtactg gtcgacgtcg
     6241 atcgaacgga cgtgatcgac cgtctcctta accgctgcca ttggcctctc ctctccggtt
     6301 tcaaggaccg gggggttggg atgtatgcac tgtccttgcc ccgtcaggcc gccgactgcc
     6361 gccgcggcgc aacccgcgtc agctgccggc gcacactgtc gagcgcctga tttatgtcgt
     6421 cttcgtccga ggtccagccc aggctgacgc ggatcgcgcc gtccgccagc gcagaactga
     6481 tgcccatcgc atccagaacg gcattgcgtc ccaccttgcc ggaggagcag gcggagccgc
     6541 tcgacaccgc cacgccatcg agatcgaagg cgatcaccgc cgtttcggcc ctcaggccag
     6601 gaatggcgaa caaactggtg ttggggagcc gggtggcctg cgcgccaaac accacgacat
     6661 ccggtgccag cgaccgcagg tcccgctcga accggtctcg cagggtccgc acgcgctcca
     6721 tctccggcag gcgcgcctgc gccagccgtg ccgccacgcc gaagccgacg atgccggcaa
     6781 cattctccgt ccccgcgcgc aggccacgct cctgtccgcc cccgcgcagc agcggcctga
     6841 cgccgccgca ggcatccgcc cgagcgatca gcgcgccgac gcccttcggt ccgccgatct
     6901 tgtgcgccga gagcagcagg aaatccgcac cgagagtgcg cgcgtcgagc ctcagtttcc
     6961 cggcggcctg cacggcgtca caggtgacgg tgaacccctg cgctttgagc cgggcgatgg
     7021 cattgccatc gttgaggacg ccggtctcgt tgttcgccgc ctgcaccgcg acgtggatga
     7081 ggtccggccg tgccctgccg ccggcgactt cggcaagagc ggcaaagtcg aagcggccag
     7141 cctcatcgac gggcatcacg tgccggcgaa gcgcatcgaa gccgcctcca gcgcggatgc
     7201 agggatgttc gctggcgccg acaaacagcg tctcttctgt cgacaccggt tgcaacgcca
     7261 tcgcggccgc ttcggtcgcg cccgaggtga aggtcacctg ccccggttcg gcgcccacca
     7321 gcgccgcgac ctcggcccgc gccttttcga tcgcagcccg cgcggcgcgc ccttcggcgt
     7381 gcaccgagga cggattgccg agttcaaggg cgcgcgcgac cgcttccgca acgtccggaa
     7441 gtaccggtgc ggtggcgttc cagtcgagat aggcgcgccg tcgtgccaag tcctgcaacc
     7501 cgaggaaaaa acgatcccac accacaactt atgcgcgatc atgagaactc ttctaacagc
     7561 acttaggcct gaggtgacac cccgtcaagg tttcggcccg gcttccggtg cggacggctg
     7621 aagcacgtat ttcgtcagtt ggaaatactg gttgaagcgg ctgccgccgg gcacccacgc
     7681 gcagtcgtcg tgatcgaacg gcgcgagcat ccacgtcaca gtcccgccca ggtagacggc
     7741 ataccccgat gttgccaggg tcgtggcatc ctgccgatcg acgatgcgcc gtgtgaagcg
     7801 tttccaggac cgggcctgat cggtcgtctc gacgaccatg taccgggcag tcggctcatc
     7861 gatcgtttga acgctcaccg atccgtcggg attcgcgctg acacgctccc agcctccgga
     7921 ccggctgcgc cgttcgatcc agcgaaagcc ctccagcaga tggctggtgc cgaagtcgcc
     7981 ggcgtgggcc gattcgagca ggaagccatc cgcctgcacc cgcttcacga cctgccccga
     8041 tgccgctgca tggcaggcct tgtggaattc gccggtgcta tggacgcggt cggccagggg
     8101 cggccccagc accagcaccg gcgccagcag gaaggaccag cgcggcccgt ggcggtacag
     8161 caaccagccg acgacgccga tcagcaccgc taggacaacc ggcatcacca gaaagatgag
     8221 accgaacggg tgtagcatgg tttgcccccg ggactggcaa cggaaagacg agcgtcgcag
     8281 catagccgga ccgcggcctg cgtcggtgcg cccgctcacc tgccgcatcc tgtcgaaata
     8341 tcttgcattt tgccggtccc cgcatgctag aaggccggcc ttgcccgcac tcaaccgggg
     8401 tcatcccatg cccgagatca tcttcaacgg tcccgccggc cgcctcgaag gccgctatca
     8461 gcccgccaag cagcggggcg cgccgatcgc gatcatcctg cacccgcatc cccagttcgg
     8521 cgggacgatg aacaaccaga tcgtctacaa cctctattac acgttcgccg atcgcggctt
     8581 ctcggtgatg cgcttcaatt ttcgcggcgt cgggcgcagt cagggcgtgt tcgatcacgg
     8641 tcagggcgag ttgtcggatg ccgctgccgc gctggactgg gctcaggccc tcaacccgga
     8701 cgccaagacc tgctggatcg caggcatttc cttcggcgcc tggatcggca tgcaactgct
     8761 gatgcgccgg cctgagatcg agggcttcat ctccatcgct gcgatggcga accgctacga
     8821 tttctcgttc cttgcgccct gcccgtcgtc cggcctgttc gtgcatggcg accaggaccg
     8881 cgtcgcgccc tacaacgagg tcatcagcgt catcgacaag gtgaagacgc agaagggcgt
     8941 caagatcgag cacaccttga tcaagggcgc gaaccacttc ttcgagaacc atgtcgagga
     9001 attgacgggc gtcgtcggca cctatctcga caagcgcctc ggcaccaagg ggcaggccgc
     9061 ctgatcccat gctgtcggcc gcgatcgccg ggttcctgct cggcggcagc ctgatcgtcg
     9121 ccatcggcgc gcagaacgcc ttcgttctgc ggcaaggatt gctgcgccat aatgtcttcg
     9181 ccgtcacgct gttctgcgcg gtgtccgatg cggtgctgat cgccctgggc gtcgccggct
     9241 tcggcgccta tgtcgcgtct tctcccggtc tgctgcggat cgtcggaatc gccggagcgg
     9301 cgtttctgtt cgcctatggc ggcatggctt tccgccgtgc cctccagccg tccgttctga
     9361 ccgccaccga agctccggcc ccaccgctgg ccactgcact ggcgagttgt gcgtccttca
     9421 ccttcctgaa cccgcacgtt tacctcgaca cggtggtgct cgttggcggc ctagccgcga
     9481 cctggccggg atcgtcgcgc tatgtgttcg gtgcgggcgc catcctcgcc tcgttcgtgt
     9541 ggttcttcgg cctcggctat ggcgcgcgcc tgcttgagcc ggtgttccgc agtccgcggg
     9601 cctggcaagt gttggattgc ctgatcggcc tgatcatgtg gatgctcgcg gcaaggatcg
     9661 cgctcccttt gttttgacgc cgccgccagg atcggaaata agggccgctg ttttccgttc
     9721 gaaccgatgg ccggccgatg accacctaca agtccgattt cctcgccacg ctctctgagc
     9781 gcggcttcat ccaccagtgc tcggacttcg aggggctgga cgcgaaggcg gccaagggcg
     9841 aggtcgtggc ctatgtcggc tacgactgca ccgcgccctc gctgcacatc ggcaatttcc
     9901 tctccctgat gatgctgtac tggctgcaac agtcgggcaa caagccgatc acgctgatgg
     9961 gcggcggcac cacgcgcatc ggcgacccct ccggcaagga cgaggcgcgc gccctgcgct
    10021 ccatcgagga gatcaattcg aacaaggctg cgatcagggg cgtcttcgcc aagatcctga
    10081 gcttcggcga cggccggacc gacgccgtca tgatcgacaa tgcggaatgg ttgccggaac
    10141 ttaagtggat tgacatgttg cgcgatgtcg gccgccattt ctcggtcaac cgtatgctga
    10201 ccatggattc ggtgcgcctc cggctcgagc gcgaacatga gatgagcttc atcgagttca
    10261 actacatgat cgcccagtcc tacgatttcg tggaactgtc gaagcgcctc ggctgcaacc
    10321 tgcagatggg cggctccgat cagtggggca acatcatcat gggcgtcgat ctcggccgcc
    10381 gcatgggcac gcatcagctc tatgccctga ccacgccgct gctcaccacc gcctccggcg
    10441 ccaagatggg caagaccgct caaggcgccg tctggctcaa tgccgagcag ttctcgccgt
    10501 atgacttctg gcagtactgg cgcaacaccg aggacgccga tgtcggccgc ttcctcaagc
    10561 tgttcaccac cctgccgatg gcggacatcc gcaagctgga agcgctcggc ggcgcggaga
    10621 tcaacgaggc aaagaaggcg ctcgccacgg cggccaccac cctgctccat ggcgcagcgg
    10681 cagccgaaca ggcagcagag accgcccgca tcaccttcga acagggcgcg ctcgccgcgg
    10741 acctgccgag cgtcggcgtg ccgaaggcgg agcttgcggc cgg
//
LOCUS       DAOMXN010000069        10531 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_3365_length_10531_cov_11.684517, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000069 DAOMXN010000000
VERSION     DAOMXN010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 10531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10531
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3365_length_10531_cov_11.684517"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="PK812_06790"
     CDS             complement(<1..197)
                     /locus_tag="PK812_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69299.1"
                     /translation="MLWTRAGCLGVLGLWILATAGWMTVTVDINARRVFAEQGLAYYT
                     TVPARFVAPADDRVSAASAPAR"
     gene            546..2477
                     /gene="asnB"
                     /locus_tag="PK812_06795"
     CDS             546..2477
                     /gene="asnB"
                     /locus_tag="PK812_06795"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HRJ69300.1"
                     /translation="MCGITGFLDTRREAGIQWLEATARAMASAMPHRGPDDDGVWCDP
                     QAGIALGFRRLAIIDLSPAGHQPMVSHSGQSALVYNGEIYNAEEIRPTLTDRGVRFRG
                     HSDSEVFLEYAEAFGFEAAVRKTVGMFAMCWHDRRDRSTWLARDRMGEKPLYVARLGQ
                     TILFASELRCFRQHPEFRAEIDPEGLSAYVRFGYVPHPHSIYRNVQMVPPGGLVRISP
                     DGEVKIGRYWTPEATAAAAKQTPFQGSESDAVDALEAVLRQAVRSCMVSDVPLGAFLS
                     GGIDSSTVVALMQAQSSRPVKTFSIGFHVEGFDEAPHARAVARHLGTEHEELYFSAQD
                     MIDLVPRLPEIWDEPFADSSQLPTYMVSAMARRHVTVALSGDGGDELFAGYSRYDQIA
                     RVAAAVNASGGLAAHLGGFVHRLMRMPAFAPMRAMMPPVFRARVDRWSGRVAEMRAEN
                     GLETAYRRTVAQGVAPQDILLAPSERVADIWQGALATNFPGAIERAQMIDTLTYLPDD
                     ILVKVDRASMAVSLEARAPLLDHRVVDFAWRLPENLKRRDGEGKWALKQVLYRHVPRA
                     LVERPKMGFGVPIDVWLRGPLRDWAEDLISERTLKADGLFRADAVRTLWNRHLAGEQW
                     QYPLWTVLMLQAWRRRWGH"
     gene            3309..5777
                     /locus_tag="PK812_06800"
     CDS             3309..5777
                     /locus_tag="PK812_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69301.1"
                     /translation="MILQALSLSCVLTLGLASSGMLVGHHRVEPAAVYKVSDKIHHYS
                     VMNYLPGTSFSSAEPRVLSRRTTLSDIQAPFIPEHMDTRFEAEAGGRANFWRSLIRFT
                     FRHPGGPQAVENALSVHGTLRLSAEAATVLLLLALVAAVGQRGLVMRAHAGSGATVSA
                     ALPRAAVGLGLGLCLMAAGLALGSGVLPPGARPMVVGLALPQGPAALLPLALAGLIAA
                     LLSWRPRMGRALRTGGIVLLIGFALVAPLLNLADVWTSGGSIERLARSPIHAFGMLPV
                     SDANDYLSGALNLLATGELNSWNSRRPLTATLLALFAQISGQDLSVVMVARAVLVSVG
                     LALVALEASLIIGGLGALALVLACALFVAEWIPVTMSEVTGCAMGLYGLAALLAAWRT
                     NNLVAFAFGVLLTTIGLTARAGPMLVAPFIVAAGALLQRPAGLRSMMIWLAAGLAAML
                     LGVLHANYLIWLLGGVSAGFQSNFSQTLYGLAVGGKGWAQIMSDHPELFASDFQGQRE
                     SLIYHYAFLEIRRSPWRLIGALWQEFLRTPAFFYRFFEISVVNVWLMAGVVAAIGAWI
                     ATRDRWALLCLSIVLGLILSSPILMADGGERAYAGAIPAMALLVACGFNWTRRLLFQV
                     YAPAEPQPKGIAHPDLPALSPAGAPTAYTVPALAGAVLALSLIVAAPLVLRAARPPEL
                     VTSRLGQTCGAGEVRSVLRGGFDLFSVVPDPAARRLTIPEVTRADLLLAVGALDPQLG
                     RVLEPRREPYVMTLAAHAPRTERIAMTSLIVRPGGMQFDKTWPFAVACLVRIGEVDSR
                     IIGQMAVYEARFLGPYDPRTSLPR"
     gene            complement(5820..6740)
                     /locus_tag="PK812_06805"
     CDS             complement(5820..6740)
                     /locus_tag="PK812_06805"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRJ69302.1"
                     /translation="MAETGLGLSPERLAPLRCPTCRAGTLAAGGSQEALVCSACGAQH
                     LAQEGVAFLVADAGAHGDAIEKARSVNPLWYQDEQPAELVSPWRHHMRKRRLYVQGVI
                     RAELARRGLSRVPRLLDMGCGDGNHLRWLSDFAEATYGSDYNPVRLARARAQLPDATL
                     FMADILDFPAFDRSFDVIFFNHVIEHIPQDVKALETAKRLLAPGGLLILGTPNEGAWW
                     WQLAYRRDPESLRTTDHVHFYTAGLLRERMQSAGLRVEHIHHMGWGPPDWRLDGRIRK
                     YKLVDDGFEWIGRLFLKGQASSLYALARAD"
     gene            complement(6733..7908)
                     /locus_tag="PK812_06810"
     CDS             complement(6733..7908)
                     /locus_tag="PK812_06810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRJ69303.1"
                     /translation="MSRLLVIIPDRITEILEKGEFQPCYYNPGEVFDEVHILMTNDDA
                     PPPEAVQHTVGRARLFLHNYPDDLTLVDRHNRWLTPFRLRKWAKGGVEIARRIKPDMI
                     RCHGADWNTYLASRINAELGIPYVVSLHINPDENPVRRFKGPQLTAEQLRQNAFYEYL
                     EGCALGRAQLVMPVYRPILPYLTRKGISRVEVCYNILDGQNLRPKQEYRLSDPPELLY
                     VGRLFNLKDPSNILRALVDIPRARYTIVGDGPIRPDLERLAADLGLSDRVVFRPAVPN
                     AELCRMLADFDLFVIHTEHFEISKSVLEGLLCGLPMIINIRKGDPVPELQSGIVRLVP
                     NTVEAYREAIAGLLSDPQQREALGRRAFAEAQARYAPAQTEKKVSEIYRTILESRRG"
     gene            complement(7905..8747)
                     /locus_tag="PK812_06815"
     CDS             complement(7905..8747)
                     /locus_tag="PK812_06815"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRJ69304.1"
                     /translation="MTGSGQLKPFEDDEADGLVAYLTALYQGVFTPEAIRSHLANHVG
                     FGFADYAVAVTKQALPAGARVLDLGCGFGSYVLAAREAGLDAIGIELAPFEIDFARSR
                     SRRLRPQDDAERVYRVGDATRLAEEAGSFDGVTLWNVLEHIEDAGSLLTAVDALLKPG
                     GQIFIVCPNYDAVRDEAHYHVPWTPELSRDRRAARDYLVSLGRDPAYFESSIHCRTWR
                     EVVGLLEGRGYDLFDLSSRRALSLRLGNLRSILADWKGWRALRNPARHSVELAALKSG
                     RSRL"
     gene            complement(8744..9466)
                     /locus_tag="PK812_06820"
     CDS             complement(8744..9466)
                     /locus_tag="PK812_06820"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRJ69305.1"
                     /translation="MAVHERYRDFMADQRQFFDELITEDWASYASPVWDEIRRYELDK
                     IFARVQPARILDMGCGCGFHDAEMALRPFVEQVDAFDYSSKSVEKANEAYPHSKVKRF
                     VADLATFAPENRYDLVASFQVYEHLEDVGAYFDACRRACAPGGMIVIVTPNRTRLNNR
                     IRNWRGLPDELCDPQHFREYTIADIHADAARHGFEPAGGFGYGLYGWPWLERIPHRVR
                     LRLGAIVPSIAHGIAAFARAPR"
     gene            complement(9470..10261)
                     /locus_tag="PK812_06825"
     CDS             complement(9470..10261)
                     /locus_tag="PK812_06825"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRJ69306.1"
                     /translation="MSETNRPDSPRATFEAGKVYKVETICARADAQSAEYALRLLKRK
                     LEMVIAHHAEGLTLDLCCATGAHLLDLADFIRPAIGIDFSVPFVRKAADDAAAAGHSD
                     LRFLVGDAKALPLTDGSIATAYCFSSLYVIPDVHQVIDELARVLRPGGRAILDLGNAR
                     SLNAYCVRHYPDLARQHFQTRGAMRKMVQNAGLAIVEQRAFQILPLWADRPGWLWPLL
                     HPRWKRILATRVGGRMLDEWISNLPFLRSLAFRQMIVCEKPVRKD"
     gene            complement(10269..>10531)
                     /locus_tag="PK812_06830"
     CDS             complement(10269..>10531)
                     /locus_tag="PK812_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69307.1"
                     /translation="GLYDCGWIFPGASDGKQRGLTLFKTRFGGEPHRAFRARRQVEPA
                     RPSNPEPEPEPAPVGQSADERPTTAQRRLGLVRRLTRRLRFD"
BASE COUNT         1810 a         3383 c         3408 g         1930 t
ORIGIN      
        1 cgggccggcg cgctcgcggc agacacacgg tcgtccgcag gtgcgacgaa gcgggcgggc
       61 acggtcgtat aataggcaag gccctgctcg gcgaaaacac ggcgtgcatt gatgtcgacc
      121 gtcaccgtca tccagccggc ggtcgccagt atccacagcc cgagcacacc cagacacccg
      181 gcacgcgtcc acagcacgag acgatgaccc agggaggtgg cagaacggga cgggtggggc
      241 acaggatatc gatcgtcgct caagcgtggg cagacttgcc caggacaaac gctgcatgaa
      301 acacgttatg catcctcggc tgacgggtcc agaacgatga atgtccccgc agacagccag
      361 tatcgggcca tcgcaggagt gatccctcca gccaccgccg tttgcgccct gatagctcag
      421 gcctccatca ggcgccatag gccatcggac tgccggcgga tcgcggcgct tgatggatgg
      481 cccgatgatg ctatggcggg atgccgagat cgcgcgaccg aaaccggcgc caagaagggc
      541 gaggcatgtg tggcataacg ggattcctgg atacgcggcg tgaagcgggt atccagtggc
      601 tggaagcgac ggcgcgcgcg atggcatccg cgatgccgca tcgcggaccc gacgacgacg
      661 gcgtctggtg cgacccgcag gccggcatag ctcttggctt ccgtcggctg gcgatcatcg
      721 atctttcgcc ggccgggcac cagccgatgg tgtcgcattc gggccagagc gcgctcgttt
      781 acaacggcga aatctacaac gccgaggaaa tccggccgac cctgactgac cggggggtgc
      841 ggtttcgggg ccattccgat tcggaagtgt tcctggagta cgccgaggcg ttcggctttg
      901 aagcggcggt gcgaaagacg gtcggcatgt tcgccatgtg ctggcacgat aggcgcgacc
      961 gcagcacctg gctggcgcgc gaccgcatgg gcgagaagcc gctgtatgtc gcacggttgg
     1021 gccagacgat cctgtttgcc tcggaactac gctgtttccg ccagcatccg gaattccgcg
     1081 ccgagatcga tccggagggc ctgtcggcct atgtccgctt cggctatgtg cctcacccgc
     1141 attcgatcta tcgcaatgtc cagatggtgc ctcccggcgg gctggtccgc atttcgccgg
     1201 atggcgaggt gaagatcggg cgctactgga cacccgaggc cacggcggcg gccgcgaagc
     1261 agacaccgtt tcagggcagc gagagcgacg cggtcgacgc tctcgaagcg gtgttgcgcc
     1321 aggccgtccg gagctgcatg gtctcggatg tgccgctcgg tgccttcctg tccggcggca
     1381 tcgattcgtc caccgtcgtc gcgctgatgc aggcgcaatc gtcgcggccg gtgaagacgt
     1441 tttccatcgg ttttcacgtc gaaggcttcg atgaagcacc ccacgccagg gcggtggcca
     1501 ggcacctggg aaccgagcac gaggaactct acttcagcgc gcaggacatg atcgatcttg
     1561 tgccgcgcct tcccgagatc tgggacgaac ccttcgcgga ttcgtcgcaa ctgccgacct
     1621 acatggtctc ggcaatggcg cgtcggcacg tcacggtcgc attgtccgga gacggcggcg
     1681 acgagctctt cgccgggtac agccgctatg accagatcgc cagggtcgct gcggcggtca
     1741 atgcctcggg cggcctggcc gcccatctcg gcggcttcgt gcacaggctg atgcggatgc
     1801 ctgccttcgc gccgatgcgc gccatgatgc ccccggtctt ccgggcgcgc gtcgaccgct
     1861 ggtccggacg cgttgccgag atgcgcgcgg aaaacggact ggaaaccgcc tacaggcgga
     1921 ctgtggccca aggcgtcgcg ccgcaagaca tcctgctcgc accctcggaa cgcgtcgcgg
     1981 acatctggca aggcgcgctg gcgaccaatt tcccgggcgc gatcgaacgg gcacagatga
     2041 tcgacaccct gacctatctg cccgacgaca tcctggtgaa agtggatcgt gcaagcatgg
     2101 cggtcagcct tgaggcacgc gcgccgctgc tggaccatcg cgtggtcgat tttgcctggc
     2161 gcctgcccga gaacctcaag cgacgggacg gcgaaggcaa gtgggcgctg aagcaggtgc
     2221 tttaccgtca tgtcccgcgc gcgctggtgg agcgtccgaa aatgggcttc ggcgtcccga
     2281 tcgacgtatg gctgcgcggg ccgctcaggg actgggccga agacctcatc agcgagcgga
     2341 cgctgaaggc tgacgggctg ttccgggccg acgccgtccg cacgctgtgg aaccgacatc
     2401 tggccggaga acaatggcag tacccgctct ggaccgtgct catgctccag gcctggcgcc
     2461 gtcgctgggg ccattgaggc gcaggtcatg gaacgcctgg cagccccccc tttggcagcg
     2521 atcacatccg aaacatccgg tggactggcg ggcgccgcgg cgacgccggt aaggtcagcg
     2581 ccagaggcct cgcgttgcgc cggggacccg atctcatgac gctgcgaccg tcgacagccc
     2641 agtcggtggc cctgaccatc gtcgtgtttt gcacgacggt cctggccgtc tggcttgtgc
     2701 tcgcgtcagg gctgctcctg gcagagaaac ggatcgacac ttccaaggtt cggcaggacg
     2761 ccacagccgg cacgtcgcta gccgtaccga cgtcgcggtt ttaccgtttc gccgaatttc
     2821 agttcgcccc gcagccggac gcgagcgccc cggctggcac ccccagacac cactccggtg
     2881 gcaagccttc catccactcc ctattggaaa aatttttctg gtctaagcac aatgtgatca
     2941 agctgagagg gtcagataat tcaatagaaa taagagatgg cgcgttcttt acagcaatac
     3001 tttggccgtc aaatctggta tttttttgtt tgtccgcgtt gttgatgttt tcaatcaccg
     3061 tttattataa gttgcgattt gagaaccctc aggcaaggcg cccggaaagg atttacatct
     3121 tagccactgg tatcgcatcg ggccttgttt tttcaatcat accggcggtg ggggcctttt
     3181 ccgcaatccg cctgaatgcg tccatggcgc cgccgcttct cgtcgttctt gggttttctc
     3241 tgatccttgc gcggatctgc tttggaggtc cgccgcctgg gtgctctccc cgcagagcgg
     3301 cattggccat gattctccag gccttgagcc tgtcctgcgt gttgaccctc ggacttgcca
     3361 gttccggcat gctcgtcgga caccaccggg tggagccggc ggcggtctac aaggtttcgg
     3421 acaagatcca tcactactcg gtcatgaact accttccggg gacgagcttc tcgtcggcgg
     3481 aaccgcgggt cctgtcgcgg cgcacaacgc tctcggacat ccaggccccc ttcattccag
     3541 agcatatgga cacgcggttc gaagcggaag ccgggggacg ggcgaatttc tggcgctccc
     3601 tcatcaggtt cacgttccgg catccgggcg gaccgcaggc tgtcgaaaac gccctgagcg
     3661 ttcacgggac gcttcgactg agcgccgaag cggccaccgt gctgctgtta ctggcgttgg
     3721 tcgccgctgt gggtcagcgg gggcttgtca tgcgcgccca tgccggcagt ggtgcgaccg
     3781 tttcagctgc gttgccgcgc gccgccgtcg gcctaggact cggattatgc ctgatggcgg
     3841 cgggccttgc gctcggcagc ggtgttctgc cgccaggagc gaggccgatg gtggttggtc
     3901 tggccctccc ccaaggtccg gccgcattgc ttcccctggc cttggccggg ttgattgcgg
     3961 ctttgttgtc gtggcgtccg cgcatgggac gggccctgcg gaccggcggc atcgtgctgc
     4021 tgatcggctt tgcgctggtc gcccccctgc tgaaccttgc cgacgtgtgg acgtcaggag
     4081 gaagcatcga acgcctggcc cgctccccga tccatgcctt tggcatgctt ccggtctcgg
     4141 acgccaatga ctacctgtcc ggcgcgctca acctcctggc gaccggtgag ttgaacagct
     4201 ggaactcgcg caggcccctg accgcgacgc tgcttgccct gttcgcccag atctccggtc
     4261 aagacctctc ggtggtgatg gtcgcgcggg cggtgctggt ttccgttggc ctcgcgctcg
     4321 tcgccctcga ggcctccctg atcattggcg gacttggcgc gctcgcgctc gtgcttgcct
     4381 gcgccctttt cgtcgccgag tggattcccg tcaccatgtc cgaggtcacg ggttgcgcca
     4441 tgggactcta cgggctggcg gcattgctgg cggcctggag aaccaacaac ctggtggcct
     4501 tcgccttcgg tgtgctgctg acaaccatcg gcctcacggc acgcgcaggg ccgatgctcg
     4561 tggcgccttt catcgtcgcg gctggcgcgc tgttgcagcg ccctgccgga ctgcgatcga
     4621 tgatgatctg gctggcagcg gggctggcgg ccatgctgct tggcgttctc catgccaact
     4681 acctgatctg gcttctgggc ggcgtttccg ccggcttcca gagcaacttc tcgcagactc
     4741 tctatggcct ggcagtcggc ggcaagggat gggcacagat catgtcggat catcccgaac
     4801 tgttcgcgag cgatttccag gggcaacggg aatcgttgat ctatcactat gcctttctgg
     4861 aaattcgaag gtccccgtgg cgcctgatcg gggcgctctg gcaggaattc ctgcgcactc
     4921 cggcattctt ctatcgcttc ttcgagatct ccgtcgtgaa cgtctggctc atggccggcg
     4981 tcgtggccgc gatcggcgcc tggatcgcaa cacgcgatcg ctgggccctg ctctgcctgt
     5041 cgatcgtgct cggcctgatc ctctcttcgc cgattctgat ggccgacggc ggggaacgcg
     5101 cctatgccgg agccataccg gcgatggcgc ttctggtcgc ttgtggattc aactggaccc
     5161 ggcgtctgct cttccaggtg tacgcgccgg ccgagccgca gcccaagggt atcgcgcacc
     5221 cagacttgcc agccctcagt cctgccggcg cgccgacggc ctacaccgtg ccggcgcttg
     5281 ccggcgctgt gctggccctg tccctgatcg tggcggcacc actggtcctt cgcgcggcgc
     5341 ggccacccga gcttgtgacg tcaagacttg gccagacgtg cggggctggc gaggtgcgct
     5401 ccgttctgcg cggcgggttt gacctgttct cggttgtccc agacccggcg gcccgtcgcc
     5461 tcaccattcc cgaggtgacg cgtgcagact tgctgctcgc ggtcggcgca ctcgatcccc
     5521 agctgggcag ggtgcttgag ccaaggcgcg aaccctatgt gatgacactg gccgcgcacg
     5581 cgccgcgcac ggaacggatc gccatgacct cgctgatcgt tcgcccgggc ggcatgcaat
     5641 tcgacaagac gtggcccttt gcggtcgcct gcctggtccg catcggcgaa gttgacagcc
     5701 gcatcatcgg ccagatggcc gtctacgaag cgcgcttcct gggtccctac gatccgagaa
     5761 ccagcctgcc gcgataggcc ggtcgccgtt cgacaggtcg cttcgtcagc gcgccttcgc
     5821 tagtcggcgc gcgctagtgc gtagagggaa gaggcctgcc ccttcaggaa aaggcggccg
     5881 atccattcaa acccgtcgtc cacgagcttg tacttgcgga tcctgccatc aagccgccag
     5941 tccggtggcc cccagcccat gtggtggata tgctccacgc gaaggccggc gctctgcatc
     6001 cgttcccgca acaggccggc cgtgtagaaa tggacatggt ccgtcgtgcg caggctctcc
     6061 ggatcgcgac gataggccag ttgccaccac caagcgccct cgttgggcgt tcccaggatg
     6121 agcaggccac cgggagccag cagccgtttc gcggtctcga gcgctttgac gtcctgggga
     6181 atatgctcga tcacgtgatt gaagaagatc acatcgaagg atcggtcgaa ggcggggaaa
     6241 tccagaatat ccgccatgaa cagcgttgcg tccggcaact gggcgcgcgc ccgcgccaag
     6301 cgcaccggat tgtagtcgct gccataggtc gcctcggcga aatccgagag ccagcgcaga
     6361 tggttgccgt cgccgcagcc catgtccagc agccgcggga cgcgcgagag gccgcgacgc
     6421 gccaattcgg cccgaatgac cccctgaacg taaagccgac gcttgcgcat atggtggcgc
     6481 cacgggctga ccagttcggc cggctgctcg tcctgatacc agagcgggtt gacggaccgt
     6541 gccttctcga tcgcgtcgcc atgggcgccg gcatccgcga ccaggaaggc cacgccctcc
     6601 tgcgcgagat gttgcgcgcc gcaggcggag cacacaagcg cctcctggct gccgcctgcc
     6661 gcaagcgtcc cggcccggca ggttggacaa cgcagcggcg caaggcgctc cggggaaaga
     6721 cccaaccctg tctcagccac gtcgggactc caagatcgtg cgataaatct ccgaaacctt
     6781 cttttcggtc tgggcaggcg catagcgggc ctgcgcctcg gcgaacgcgc gccgccccag
     6841 agcttcgcgc tgctgaggat cggagagcag gcccgcgatg gcctcgcgat aggcctcgac
     6901 cgtgttggga accagccgga cgatgccgct ttgcagttcc ggcaccgggt cacccttgcg
     6961 gatgttgatg atcatcggca acccgcacag aaggccctca agcaccgatt tgctgatctc
     7021 gaaatgctcg gtgtggatga cgaacaggtc aaagtcggcg agcatgcggc acaattccgc
     7081 attgggcacc gctggccgga agaccacgcg atcggaaagg ccgagatcgg cggcgaggcg
     7141 ttcgaggtcg ggcctgatcg gtccgtcgcc aacgatggtg tagcgcgcgc gcggaatatc
     7201 caccagcgcc cgcaggatgt tggacgggtc cttcaggttg aacaggcgcc ccacatagag
     7261 cagttccggc gggtccgaca gacggtactc ctgcttgggt ctgaggttct gcccgtcaag
     7321 gatgttgtag cagacctcca cgcggctgat gcccttgcgc gtcagatacg gcagaatggg
     7381 cctgtagacc ggcatgacca actgggcacg tccgagcgcg cagccctcaa ggtattcgta
     7441 gaaggcattc tgccgcagct gttccgctgt aagctgcgga cccttgaaac ggcgcacagg
     7501 gttttcatca ggattgatgt gcagcgaaac cacatatgga atgccgagtt cggcattgat
     7561 ccggctcgcg agataggtgt tccagtccgc accgtggcaa cggatcatgt ccggcttgat
     7621 gcggcgcgcg atctcgacgc cgcccttcgc ccatttgcgc agccggaaag gcgtcagcca
     7681 gcggttgtgc cggtcgacca gcgtcagatc gtcggggtaa ttgtgcagga acagccttgc
     7741 cctgccgacc gtatgctgca ccgcctcggg cggcggcgca tcgtcattgg tcatgaggat
     7801 gtgaacctcg tcgaagacct cgcccggatt gtagtaacag ggctgaaatt ctcccttttc
     7861 caggatttcg gtaatccggt ccggaatgat gacgagcaga cgactcatag gcggctccgc
     7921 ccggatttga gcgctgcgag ctcgacggaa tggcgtgccg ggttgcgcag cgcgcgccag
     7981 cctttccagt ccgccaggat cgagcgcagg ttgccaagtc ggaggctgag ggcgcgccgg
     8041 ctcgacaggt cgaacaggtc gtagccgcgt ccttcgagaa gacccacgac ctcccgccag
     8101 gttcggcaat ggatcgagga ttcgaaatat gcagggtcgc ggccgagcga gacaagatag
     8161 tcgcgggccg cccttcgatc gcgcgacaac tccggcgtcc agggcacatg gtagtgtgcc
     8221 tcgtcacgaa cggcatcgta attcgggcac acgatgaata tctggccgcc cggtttcagc
     8281 agggcatcca cggcggtcag caggctgccc gcatcttcga tatgttcgag cacgttccag
     8341 agcgtcacgc catcgaagct gccggcttcc tcggcgagcc gcgtggcgtc accgacacgg
     8401 tagacccgct cggcatcatc ctgcggacgc agccgccgcg agcgggagcg ggcgaagtcg
     8461 atctcgaacg gcgcgagttc aatgccgatc gcatcaagcc cggcttcccg cgcagccagc
     8521 acatacgagc cgaacccaca gcccagatcg agcacgcgcg cgcccgccgg cagcgcctgc
     8581 ttcgtcacgg ctacggcata atcggcgaag ccaaaaccga catgattggc aagatggctg
     8641 cggattgcct cgggcgtgaa cacgccctgg tagagcgccg tgagataggc gacgagacca
     8701 tccgcctcgt catcctcgaa aggcttgagc tgtccggaac cggtcatcgt ggcgcccgcg
     8761 cgaatgccgc tatgccgtgt gcgatgctcg gcacgatcgc ccccaggcgc aggcgcaccc
     8821 tgtgcggaat gcgctccagc cagggccagc cgtagagccc atagccaaag ccgccggccg
     8881 gctcaaaccc atgccgcgcg gcgtccgcat gaatgtcggc gatggtgtac tcgcggaagt
     8941 gctggggatc gcacaattcg tccggcaggc cacgccaatt gcggatgcgg ttgttgagcc
     9001 gcgtgcggtt gggcgtgacg atcacgatca tgcctcccgg cgcgcaggcg cggcggcaag
     9061 cgtcgaagta ggcacccacg tcctccagat gctcatagac ctggaagctc gcgaccagat
     9121 cgtatcggtt ttccggggcg aaggtcgcca ggtcggcgac aaagcgtttg accttggaat
     9181 ggggataggc ctcattggcc ttttcgacgc ttttcgatga gtagtcgaac gcatcgacct
     9241 gctcaacgaa tggacgcaac gccatctcgg catcgtggaa gccacagccg cagcccatgt
     9301 cgaggatgcg cgccggctgc acgcgagcga atatcttgtc cagttcgtag cggcgaattt
     9361 catcccagac cggagaagca tagctggccc agtcttcggt gatcagctca tcgaagaact
     9421 ggcgctggtc ggccatgaag tcgcggtaac gttcgtgaac ggccatggct cagtccttcc
     9481 ttaccggctt ttcgcagacg atcatctgcc ggaaggccag tgaccggagg aacggcagat
     9541 tgctgatcca ttcatcgagc atcctgccgc cgacgcgtgt cgcgagaatc cgcttccagc
     9601 gtggatgcag caacggccag agccagccgg gacgatcggc ccagagcggc aggatctgga
     9661 acgcacgctg ttccacgatc gcaagcccgg cgttctgaac cattttgcgc atcgcgccac
     9721 gtgtctggaa atgctgccgg gcaaggtccg gatagtggcg cacgcaatag gcgttcagcg
     9781 accgggcgtt gccaagatca aggatggcgc gaccgcccgg ccgtaacacg cgcgccagtt
     9841 catcgatcac ctgatgaacg tcaggaatca cgtaaagcga ggagaaacaa taggccgtcg
     9901 cgatgctgcc gtctgtcagc ggcagagctt tggcatcgcc aacgagaaag cgcaggtcgg
     9961 aatgccctgc ggccgcggca tcgtccgccg ccttcctgac aaaaggcacc gaaaaatcga
    10021 tgccgatcgc cgggcggatg aaatccgcaa ggtccagaag atgggcgccc gtagcacagc
    10081 acagatcgag cgtgaggcct tcggcgtgat gggcgatgac catctcgagc tttcgcttca
    10141 ggaggcgcag cgcatattcg gccgactgcg catcggcgcg ggcacagatc gtctcgacct
    10201 tgtagacttt gcctgcctcg aacgtcgctc gcggactgtc gggcctgttg gtctcgctca
    10261 tgccgctctc aatcaaatcg caggcgacga gtcaaacggc gcacaaggcc gagacgtcgt
    10321 tgcgcggtcg tcggcctttc gtccgccgac tgcccgacgg gcgctggctc cggctccggc
    10381 tccgggtttg acgggcgggc gggttccacc tgccggcgcg cacgaaaggc ccggtgcggt
    10441 tcaccgccga agcgggtctt gaaaagggtc agtccacgtt gcttgccgtc gctcgcgccc
    10501 gggaagatcc agccgcaatc atacaagcca t
//
LOCUS       DAOMXN010000070        10447 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_3402_length_10447_cov_10.900789, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000070 DAOMXN010000000
VERSION     DAOMXN010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 10447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10447
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3402_length_10447_cov_10.900789"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..368
                     /locus_tag="PK812_06835"
     CDS             114..368
                     /locus_tag="PK812_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019965229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4212 domain-containing protein"
                     /protein_id="HRJ69308.1"
                     /translation="MSQSSDDVYWGKTSKLMVVMIALWVFFGYIVHMFVTPLNGIKIF
                     GFPLGFYMASQGSLIVFVAMLFWFGRSQDKIDRECGVAED"
     gene            380..2353
                     /locus_tag="PK812_06840"
     CDS             380..2353
                     /locus_tag="PK812_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019965228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HRJ69309.1"
                     /translation="MATETKAGGASFIDSLGKIYAIYTGGFFGFIVIIAILEQLGVPN
                     KYLGYMFMGLTIVVYAFIGIVTRTAEVSEYYVAGRQVPAFYNGMATGADWMSAASFIG
                     MAGSLYLLGYDGLAFVLGWTGGYVLVAVLIAPFLRKFGAYTVPDFLDARYGGNGVRLA
                     GVMVLLACSFTYVVAQIFGTGLIAQRFLDMPFEYAVFVGLLGILVCSMLGGMKAVTWT
                     QVAQYIVLIVAYLIPVVWLSAKKYGLPIPQLTYGQALSQIADLEQNFIKNGLAPATMK
                     LHAAPFTSYTPGNYFALILCLMVGTASLPHVLMRYFTTPSVREARVSVAWSLLFIFLL
                     YFTAPAYAAFSKLEVYSTLIGKNIADLPQWVYNWGKLGLVNVCGKAAASADAVKAACG
                     AISGHAGLLRWQDFGINTDVVVISTPEIAGLPYVVTGLVAAGGLAAALSTADGLLLAL
                     ANALSHDIYYKMINPNAPTKQRLIVSRVLLVIVAIFAAWTAAQRPADILAMVSWAFSL
                     AAGGLFPALVLGVWWKRANKAGAIAGILAGFGLTLFYLVITRYFPGYGVAYFGMTSLT
                     DPTGKALIADMAAAMKAANAFDSWVPLTHPLASKVGWLNMNNISSAIFGLPVGFGVMY
                     IVSMMTPEPSKQIQDFVDACRRPKGQTLMAEKS"
     gene            2444..2746
                     /locus_tag="PK812_06845"
     CDS             2444..2746
                     /locus_tag="PK812_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69310.1"
                     /translation="MTDSVGFWLFEVPNFLFAVAIYTLLGRYVLSIFFRPESDAVIWK
                     VFCQITDPIVAAIGAITPRLIPPPLVVVLAIIWLMMARIGLFIAIRWLGFIPGSPS"
     gene            2743..3084
                     /locus_tag="PK812_06850"
     CDS             2743..3084
                     /locus_tag="PK812_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69311.1"
                     /translation="MNHTEDERAAIMQKDGLLIGIAGLSLVSGMHFSPYFDPAFVLVS
                     VLLAPGFFITSKLLLFYFTSLLVALAALAIAGVPAAIFERVTGRKESDFKSMSIWLAG
                     CFVIALPALIP"
     gene            3174..3548
                     /locus_tag="PK812_06855"
     CDS             3174..3548
                     /locus_tag="PK812_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69312.1"
                     /translation="MLDIIGAKAKISVARGCGFGCLAILCMMVGMSIDLPAAFEAGGI
                     AMLMMSIILILKRRYLETISHKRTELWIMLAPAERPDEKNAKAVISAILGRTYLEFAL
                     HAAKGAVFFFLISLLLAFLRSR"
     gene            3897..5606
                     /locus_tag="PK812_06860"
     CDS             3897..5606
                     /locus_tag="PK812_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007564841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRJ69313.1"
                     /translation="MATTTAAAGAAPRPMTREEKRVILASSLGTVFEWYDFYLYGSLA
                     AMIGAKFFSAFDVNTRSIFALLAFAAGFLVRPFGALVFGRLGDLIGRKQTFLMTILIM
                     GLSTFFVGLLPDYNTIGWIAPVTLIALRMLQGLALGGEYGGAAVYVAEHAPVGRRGFY
                     TSWIQTTATLGLLLSLFVIVGLRIWMGEKAFADDFPLFFGLQGGWRIPFLGSIFLLAV
                     SVWIRLQMQESPAFQKMKEEGTQSKAPLSEAFGQWKNLKIVIIALVGLVIGQAVVWYT
                     GQFYALFFLQSILKIDLLTANVLIAWSLIIGTGGFIFFGSLSDKVGRKPIILGGCLIA
                     ALTYFPLFKMMAETANPNYAKAIETVKVEVSSDPAKCGSLFDPVGIRQFTEPCDLARR
                     VLSQQAIKYEYKPGTAGQPLKVSVNGKEAAWGAGLADFTKNIVAEATAAGYPGAADKD
                     KVKVDGFFAALSDAKALKIIGILTILVLFVTAVYGPIAAALVEFFPTRIRYTAMSLPY
                     HIGNGWFGGLLPATSFAMVASTGDIYFGLWYPIGFALMTFVIGLLLVKETKDRDINTF
                     TGN"
     gene            5747..5821
                     /locus_tag="PK812_06865"
     tRNA            5747..5821
                     /locus_tag="PK812_06865"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5779..5781,aa:Thr,seq:tgt)
     gene            5837..6718
                     /gene="folD"
                     /locus_tag="PK812_06870"
     CDS             5837..6718
                     /gene="folD"
                     /locus_tag="PK812_06870"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HRJ69314.1"
                     /translation="MTASQIIDGKAIAAATRETVAAAVAEITARHGIRPGLHVVLVGD
                     DPASKVYVGAKEKMAEKAGFVSVVHRLPAHTAESELLAVIARLNADTGVDGILVQLPL
                     PRHVDTNRIIDAIDPAKDVDGFHLVNVGKLSAGRAALVPCTPQGCMILLRSVRTDLSG
                     LNAVVVGRSAIVGKPLAQLLLAADCTVTIAHSRTRDLATICRGADILVAAVGRPEFVK
                     GDWVKPGAIVIDVGINRLEDGRLVGDVDFASAATVAGAITPVPGGVGPMTIACLLRNT
                     AIAALDRRGLPRPAALA"
     gene            6924..7895
                     /locus_tag="PK812_06875"
     CDS             6924..7895
                     /locus_tag="PK812_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRJ69315.1"
                     /translation="MDISRRTLMAGAAALGAAGTLPNAGEAKAPLAGKQNAGFYRTKI
                     GDFEVTTINDGFFARPAAGFVKNAMDDEVKAAISAARLPAGPDNVFIPFTTVVVNTGS
                     KLVLIDCGNGNSGAPTSGTWMANFKAAGFDPKDVDAVIISHFHGDHINGIRLKEGTAV
                     FPNAELMVPAAEWGFWMDDARMNNAPDPMKPAFQNVRRVFGPSAKDVKQFEPGKEVVA
                     GITAVAAPGHTPGHNVFMVSSGNGKLLCLADTTNNPHLFVRNPDWQAVFDMDGAAAAA
                     TRRKLLDMVVAEKTQVHLYHWPFPATGFVEKDGAGYRVVPATWTGAI"
     gene            8008..8793
                     /locus_tag="PK812_06880"
     CDS             8008..8793
                     /locus_tag="PK812_06880"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004448174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HRJ69316.1"
                     /translation="MRLVTQAPAARIAFIASSAPEAVAARDRLARYYGDAKAENADII
                     VALGGDGLMLQTLHRFMGSGKPIYGMNRGSVGFLMNEFSEMGLPERLLAAERSVVHPL
                     LMRATTVSGEIRESHAVNEVSLLRQSYQAAKLSIAIDGKERMPELICDGVLVATPAGS
                     TAYNLSANGPILPLNAPLLALTPISAFRPRRWRGALLPERAAVTISVLEADKRPVSAV
                     ADHVEIRSVARVDVAMDRRIDLVMLHDPGHSLDERILREQFGV"
     gene            complement(8801..10285)
                     /locus_tag="PK812_06885"
     CDS             complement(8801..10285)
                     /locus_tag="PK812_06885"
                     /inference="COORDINATES: protein motif:HMM:NF021603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HRJ69317.1"
                     /translation="MTQAYVAGHLGSDSREDAEAALTLILDDPSMQVRRALAWELAAC
                     ESAPRHIIVALAHDQPEVAALVLARSPLLREADLIDCAANGTVLTQTAIALRPLVPAR
                     VSAVLAATAEPAALISLLRNPGGEIAAASFAAMIERYPGEASLREALNERSDLPATVR
                     HAVMRGVSADLMAFAAGWMPPQKAERAIRETQEIATVGLAAEADDLGEMVDYLCRAGH
                     LTPGLLLRSLLCGEVALFGAALTRLGGLNARRVAGMLTARNQGALSAACLKAGLPRTY
                     LPAFLAAVSAVRDMGTASGETPRLNRLVIQRVLVACLEQGEAEMNQLLALLRRYDAQA
                     AREDARLATAQIMLAEAPVTLDSVERPDVPDFSALEQALDDDPDRPFTEAELRESGSS
                     IVDLERFGEDLVLQADDLVEPALAAQSDADGAFVADFDADFDALVQSAFHEQNLSGVS
                     SANNSAGREPLPARTVEPLRVIESQAQHLDESYFNSWELRRSVA"
BASE COUNT         1785 a         3435 c         3174 g         2053 t
ORIGIN      
        1 attccctaac ggaaaattaa tttcgaccca gcgtgccgca gagtgcgcca acttggaaac
       61 gtccaaccga ccgctctcgt gcggccgtat cggtttggca tttggagggc ttgatgagtc
      121 aaagctcaga tgacgtctac tggggcaaaa ccagtaagct tatggtcgtg atgatcgcgc
      181 tgtgggtatt cttcggctac attgtccaca tgttcgtgac gccgcttaac ggcatcaaga
      241 ttttcggctt cccgctgggc ttttacatgg cctcgcaggg ttctctgatc gtattcgtcg
      301 cgatgctgtt ctggttcggc aggtcccagg acaagatcga ccgtgaatgc ggcgtggctg
      361 aagactgagg agcgcaaaca tggcaacgga aacaaaagcc ggcggcgcct cgtttatcga
      421 cagcctcggc aagatttacg cgatctacac cggtggattc ttcggattca tcgtcataat
      481 cgcgatcctc gaacagctgg gcgtacccaa caagtacctc ggttacatgt tcatgggcct
      541 gacgattgtc gtctatgcct tcatcggcat cgtcacccgc accgccgagg tgtcggagta
      601 ttacgtcgcg ggccgtcagg tgccggcgtt ctacaacggc atggcgacgg gcgcggactg
      661 gatgtcggcc gcctcgttca tcggcatggc cggttcgctg tacctgcttg ggtatgacgg
      721 ccttgccttc gtgctgggct ggaccggcgg ttacgtgctt gtggcggtgc tgatcgcgcc
      781 gttcctgcgc aagttcggcg cctatacggt tccggacttc ctggatgccc gttacggcgg
      841 caacggcgtg cgccttgccg gcgtcatggt gctgctggcc tgctccttca cctacgtggt
      901 ggcgcagatc ttcggcaccg gcctgatcgc ccagcgcttc ctcgacatgc cgttcgaata
      961 cgcggttttc gtgggccttc tgggcattct ggtgtgctcg atgctcggcg gcatgaaggc
     1021 cgtgacctgg acgcaggtgg cgcagtacat cgttctcatc gtcgcctacc tcatccccgt
     1081 ggtgtggctg tcggcgaaga agtacggcct gccgatcccg cagctcacct acggacaggc
     1141 gctgtcccag atcgctgatc tggaacagaa tttcatcaag aacggcctcg cgcccgccac
     1201 gatgaagctg cacgccgcgc cgttcaccag ctatacgccc ggcaactact tcgcgctgat
     1261 cctctgcctg atggtcggca cggcgtcgct gccgcacgtt ctgatgcgct acttcaccac
     1321 gccgtccgtg cgcgaagccc gcgtctcggt ggcctggtcg ctgctgttca tcttccttct
     1381 gtacttcacc gctccggcct atgcggcgtt ctccaagctc gaggtctact cgaccctgat
     1441 cggcaagaac atcgcggacc tgccgcagtg ggtctacaac tggggcaagc tcggcctcgt
     1501 caacgtctgc ggcaaggctg cggcctccgc cgacgcggtc aaggcggcct gcggcgccat
     1561 ctccggccat gccggcttgc tgcgctggca ggacttcggc atcaacaccg acgtggtggt
     1621 gatctccacg ccggaaatcg caggccttcc gtatgtcgtc accggcctcg tggccgccgg
     1681 cggcctcgcg gcggcgctct cgaccgccga cggtctgctc ctcgccctcg ccaacgcgct
     1741 gtcgcatgac atctactaca agatgatcaa cccgaacgcg ccgaccaagc agcgcctgat
     1801 cgtttcgcgc gtcctgctgg tcatcgtggc gatcttcgcg gcctggacgg cggcgcaacg
     1861 gccggcggac atcctcgcga tggtctcctg ggcgttctcg ctggccgcag gcggcctctt
     1921 cccggcgctg gtccttggcg tctggtggaa gcgcgcgaac aaggcgggcg ccatcgctgg
     1981 catcctggct ggtttcgggc tcaccctgtt ctacctggtg atcacccgct acttcccggg
     2041 ctacggtgtc gcctacttcg gcatgacctc gctgaccgat ccgaccggaa aggcgctgat
     2101 tgccgacatg gccgcggcca tgaaggcggc caacgcgttc gacagctggg tgccgctgac
     2161 gcacccgctg gcgtcaaagg tcggctggct caacatgaac aacatctcct cggccatctt
     2221 cggcctgccc gttggcttcg gggtgatgta catcgtgtcc atgatgacgc cggaaccgtc
     2281 caagcagatt caggacttcg tcgacgcctg ccgtcgtccg aagggccaga ccctgatggc
     2341 cgagaagagc tgatccggac tttcgtccgc tcaatggaag gggcgggttg caacccgccc
     2401 ctttcttttt ctagaacgtc cgggagttcc gggaggaaaa acgatgaccg actctgtcgg
     2461 cttctggctt ttcgaggtgc cgaactttct gttcgcggtg gcgatctaca cgctgctcgg
     2521 gcggtacgtt ctgtccatct tcttcaggcc ggaaagcgac gccgtgatct ggaaggtctt
     2581 ctgtcagatc accgacccga tcgtggccgc catcggcgcc atcacaccgc ggctgattcc
     2641 gccgccgctg gtggtcgttc ttgccatcat ctggctgatg atggcgcgaa tcggcctgtt
     2701 catcgccatc cgctggctcg gtttcattcc aggatctcca tcatgaacca cactgaggat
     2761 gaacgcgccg cgatcatgca gaaggacggg ctgctgatcg gcatcgccgg cctgtcgctc
     2821 gtgtccggca tgcacttttc gccctatttc gacccggcgt tcgtgctggt ttcggtcctg
     2881 ctcgcaccgg gtttcttcat cacttcgaag ctgctgctgt tctatttcac ctcgttgctc
     2941 gtggccctgg cggcgcttgc gatcgcgggc gttccggcag ccatcttcga acgtgtcacc
     3001 ggccgcaagg aaagcgactt caagtcaatg agtatctggc tcgccggctg ctttgtcatc
     3061 gcgctccctg ccctgatacc ctgaccacaa ttgcgtgaag tgccgccgaa ccgcttgaac
     3121 ggcgcctcgc gcttaagaca ccttgcttcc gttgactttg gaacggacgc atcgtgttgg
     3181 atatcattgg tgccaaggcc aagatttcag tggcacgcgg ctgtggcttc ggttgccttg
     3241 ccatcctgtg catgatggtg ggcatgtcga tcgatctccc cgccgcgttc gaggctggcg
     3301 gcatcgccat gctgatgatg agcatcatac tcatcctcaa acgacgctat ctcgagacga
     3361 tttcgcacaa gcgcaccgaa ctttggatca tgctggcgcc ggcggaacgg cctgacgaaa
     3421 agaatgcaaa ggcggtgatt tccgcgatcc tcgggcgaac ctaccttgag tttgccctgc
     3481 acgcggcgaa gggggcagtg ttctttttcc tgatttccct tctgctggct ttcctgcgat
     3541 cccgttgacg cgcgcaaggg tgccgtggac aggtgccgca gtccgcaacc tatccatttg
     3601 acgcatagac cttagacgca cgaccaactg ccaaaagaca gacgcatacc tcgaagcgta
     3661 tacgaaccct taacggcgga cagaagcatc cgccgataac cggtcccgat cgatgcaacc
     3721 tgtatcgccg gggcttgagc tggaggcgcg gcgcgacaag ggctcccggg acacgggatc
     3781 gttgggcgtt aggcctctca accatcgtca accgccgcgc ggcgaacctc ggttctccga
     3841 acggcttgtc attgccgaag ctcaggttcg gcacatcgct caagaagggc atcaacatgg
     3901 cgaccaccac cgcagcggcc ggggcagctc cccgcccgat gaccagggag gagaagcgcg
     3961 tcattctcgc ttcctccctc ggcaccgtct tcgaatggta tgatttctac ctctacggct
     4021 ctctcgccgc gatgatcggc gcgaaattct tctcggcctt cgacgtcaac acgcgctcga
     4081 tcttcgcgct gctggccttc gccgccggct tcctggtgcg tccgttcggc gcgctggtgt
     4141 tcggccgcct cggcgacctg atcggccgca agcagacctt cctgatgacc atcctcatca
     4201 tgggcctgtc gaccttcttc gtcggcctgc tgcccgacta caacacgatc ggctggatcg
     4261 cgccggtgac gctcatcgcg ttgcgcatgc ttcagggtct ggcgctgggc ggcgagtacg
     4321 gcggcgcggc ggtctatgtg gcggaacatg ctccggtcgg ccggcgcggc ttctacacct
     4381 cgtggatcca gacgaccgcg acccttggcc tgctcctgtc gctgttcgtg atcgtcggcc
     4441 tgcgcatctg gatgggcgag aaggcgttcg cggatgactt cccgctgttc ttcggcctgc
     4501 agggcggctg gcgcattccg ttcctcggct cgatcttcct gctggcggtc tcggtctgga
     4561 ttcgcttgca gatgcaggaa tccccggcct tccagaaaat gaaggaagag ggcacccagt
     4621 ccaaggctcc gctctcggaa gctttcggcc agtggaagaa cctcaagatc gtgatcattg
     4681 cgctcgtcgg ccttgtcatc ggccaggcgg tggtgtggta cacgggtcag ttctatgcgc
     4741 tgttcttcct ccagagcatc ctgaagatcg acctcctgac ggccaacgtg ctgatcgcct
     4801 ggtcgctcat catcggcacc ggcggattca tcttcttcgg ctcgctgtcg gacaaggttg
     4861 gccgcaagcc gatcatcctc ggcggctgcc tgatcgcggc gctcacctac ttcccactgt
     4921 tcaagatgat ggcggaaacg gcgaacccga actatgccaa agcgatcgag accgtaaagg
     4981 ttgaggtctc gtccgatccg gccaagtgcg gctcgctgtt cgacccggtc ggcatccgcc
     5041 agttcaccga gccgtgcgat cttgcccgcc gtgtcctctc gcagcaggcg atcaagtatg
     5101 agtacaagcc tggtactgct ggccagccgc tgaaggtgag cgtcaatggc aaggaagcgg
     5161 cctggggcgc tggcctcgct gacttcacca agaacatcgt ggccgaggcg acagcggcgg
     5221 gttatcccgg cgccgccgac aaggacaagg tgaaggtgga tggcttcttc gccgcgctgt
     5281 ctgacgcaaa ggccctgaag atcatcggca tcctgacgat cctcgtgctg ttcgtcacgg
     5341 cggtttatgg tccgatcgcg gccgcgctgg tcgagttctt cccgacccgc atccgctaca
     5401 ccgcgatgtc gctgccctac catatcggca acggctggtt cggcggtctg ctcccggcga
     5461 cctccttcgc catggtggcc tcgacgggcg acatctactt cggcctctgg tatccgatcg
     5521 gcttcgcgct catgaccttc gtcatcggcc tgctgctggt caaggaaacc aaggaccgcg
     5581 acatcaacac cttcacgggc aactgatccc gcccgggacc gggaagaccc cttcccgcaa
     5641 gaaaaccgaa gccccgccct gtgcggggct tcttcttttc aaagacgcat cgcccttgcc
     5701 gccgcgtttc ctgcctgcta acccgcctcg tcgaaactga taccgggccg gcttagctca
     5761 gcggtagagc agcggttttg taaaccgaag gtcgggggtt caatcccctc agccggcacc
     5821 attcgcgaag gtccgcatga cggcttccca gatcatcgac ggcaaggcca tcgccgccgc
     5881 cacccgagaa accgttgccg ccgcggtcgc cgagatcacc gcgcgccacg gcatccgccc
     5941 gggcctgcat gttgtgctcg tcggagacga tcccgccagc aaggtctatg tcggcgccaa
     6001 ggaaaagatg gccgagaagg ccgggttcgt ttcggtcgtt caccgactgc cggcgcacac
     6061 cgccgagagc gaactgctgg cggtgatcgc ccggctgaat gccgacaccg gcgtggatgg
     6121 catcctggtg cagctgccgc tgccgcgcca tgtcgacacc aaccgcatca tcgacgccat
     6181 cgatccggcc aaggatgtgg acgggttcca tctcgtcaat gtcggcaagc tgtcggccgg
     6241 ccgcgctgcg ctggtgccct gcacaccgca aggctgcatg attctcctgc gcagcgtgcg
     6301 cacggatctt tccggcctca atgccgtcgt cgttggacgc tccgcgattg tcggaaaacc
     6361 gctggcccag ctcttgctgg cggcggactg caccgtcacc atcgcccatt cccgaacgcg
     6421 tgaccttgcc accatctgcc gcggcgcgga catcctggtt gccgcggtcg gccggccgga
     6481 gttcgtcaag ggcgactggg tgaaaccggg ggccatcgtc atcgatgtcg gcatcaatcg
     6541 tcttgaggat ggccggcttg ttggagacgt cgactttgcc agtgcggcga cggtcgccgg
     6601 cgcgatcacg ccggtgccgg gcggagttgg tccgatgacc atcgcctgtc tgctgcgcaa
     6661 caccgcgatc gctgcgcttg accgccgcgg cctgccgcgt ccggcggcgc tggcctgata
     6721 cccacagcca cacactgcgg ccgacgaaga cttatgagtt tccattgttt ttcaatggga
     6781 agtgagaagt caaggcagcc gccaagggtc tggtttcatg gcccctggta tgacgtattt
     6841 gtgattccct cgcgccgcgt ttcaatgcag attgtcgcct ccgcttgtgt tcgtctgacg
     6901 caggcatcct tgggaggcat agaatggaca tttctcgcag aaccctgatg gccggcgcag
     6961 ccgccctggg cgcggccgga acgctgccga acgctggcga ggccaaggcg cccctggcag
     7021 gcaagcagaa tgccggcttt taccgaacca aaatcggtga cttcgaagtc acgacgatca
     7081 acgacggatt cttcgcccgc cccgcagccg gctttgtgaa gaatgcgatg gatgacgagg
     7141 tcaaagcggc catctccgct gcgcgcttgc ccgccggccc ggacaacgtc ttcatcccct
     7201 tcacgacggt tgtcgtgaac accgggtcga agcttgtgct gatcgattgc ggcaatggca
     7261 attccggcgc accgacgtcg ggcacctgga tggcgaactt caaggccgcc ggctttgatc
     7321 caaaagacgt cgacgcggtc atcatcagcc atttccacgg cgaccacatc aacggcatcc
     7381 gcctcaagga aggcaccgcc gtcttcccga atgcggaact gatggttcca gcagcggaat
     7441 ggggcttctg gatggacgat gcgcgcatga acaatgcgcc cgatcccatg aagccggcgt
     7501 tccagaatgt tcgtcgcgtg ttcggcccct cggcaaagga cgtcaagcag ttcgagccag
     7561 gcaaggaagt ggtggccggc atcacggcgg tcgccgcccc cggccacaca ccggggcaca
     7621 acgtcttcat ggtttcgagc ggcaacggca agctgctgtg cctcgccgac accaccaaca
     7681 acccgcatct ctttgtgcga aacccggact ggcaggccgt gttcgatatg gatggggccg
     7741 ccgcagccgc gacacgccgc aagctgctgg acatggttgt cgccgagaag acgcaggtgc
     7801 atctctacca ctggcccttc cccgcgaccg gattcgtcga gaaggacgga gctggctatc
     7861 gcgtcgttcc ggcaacctgg accggcgcca tctgaagcgt cacgcttgcg ctgaccgtga
     7921 agaagggcgc ctcggcgccc ttttttctcg cgcatgcgat ggacggcggc aggccgctgc
     7981 ctatgatgcg aagaacactc aggactcatc cgcctcgtga cccaggcccc cgccgcccgc
     8041 attgccttca tcgccagttc cgccccggaa gcggtcgctg cgcgggatcg tcttgcccgc
     8101 tactacggcg atgccaaagc tgaaaacgcc gacatcatcg tcgcgctggg cggcgatggc
     8161 ctgatgctcc agacgctgca tcggttcatg ggatcgggaa agccgattta cggcatgaac
     8221 cgcggttcgg tcggcttcct gatgaacgag ttcagcgaaa tgggactgcc cgagcgtctg
     8281 ctcgccgccg aacgctccgt ggtccatccc ctgctgatgc gggcgacgac ggtttctggt
     8341 gaaatccgag agtcgcacgc cgtcaacgag gtgtcactgc tccggcaatc atatcaggcg
     8401 gccaaattgt cgatcgcgat cgatggcaag gagcgcatgc cggaactgat ctgcgacggc
     8461 gtgctggtgg caacgccggc cggctcgacg gcctacaatc tttcggcaaa tggcccgatc
     8521 ctgccgttga atgcgccgct gctggccctc accccgattt ccgccttccg ccccagacgg
     8581 tggcgcggcg cccttctgcc ggagcgggcg gcggtgacca tatctgtgct ggaggcagac
     8641 aaacgcccgg tctccgcagt cgcggaccat gtcgagatcc gcagcgtggc ccgtgtcgat
     8701 gttgccatgg accggcggat cgacctggtg atgctgcacg atcccggtca cagcctcgac
     8761 gaacgcatcc tgcgcgagca gtttggcgtc tgaacgccga tcaggccacg ctccttcgca
     8821 attcccagct gttgaaatag ctctcgtcga ggtgctgagc ttgggattcg atgacgcgca
     8881 gcggttcgac agtgcgcgcc ggcaggggct cgcgaccagc cgaattgttg gcgctggaga
     8941 cgccggacag gttctgctcg tgaaatgccg actgcacgag agcgtcgaaa tccgcgtcga
     9001 aatccgcaac gaacgcacca tccgcatcgc tctgcgcagc gagtgccggt tcgacaagat
     9061 catccgcttg cagcaccagg tcttctccga agcgctcgag atcgacgatg ctcgagccag
     9121 actcccgcag ttccgcttcc gtgaagggcc ggtcgggatc gtcgtcgagc gcctgctcaa
     9181 gcgccgaaaa atcggggaca tcaggacgct cgacactgtc gagcgtgacc ggcgcttccg
     9241 cgagcatgat ctgggcagtg gccaagcgcg catcctcacg cgcggcctga gcgtcatagc
     9301 gacgcaacag ggccagcagc tgattcattt ccgcttcgcc ctgttcgagg catgcaacca
     9361 gcacccgctg tattaccaga cggttgagcc tgggtgtctc gccgctggcc gtacccatat
     9421 cgcgaacggc cgacacagcc gccagaaacg caggcagata cgtgcgggga aggccagcct
     9481 tcagacaggc ggcgctcagc gccccctgat tgcgcgccgt cagcatgccg gcgacgcgac
     9541 gggcgttcag tccgcccaac cgtgtcaatg cggcaccaaa cagggctact tcgccgcaca
     9601 gcaggctgcg caacaagagc cccggcgtca aatgtccggc ccgacacagg tagtcgacca
     9661 tctcgccaag gtcgtcagcc tcggcagcga gccctaccgt ggcgatttcc tgggtctctc
     9721 tgatggcccg ttctgccttc tggggaggca tccagccagc ggcgaacgcc atcaaatccg
     9781 ccgacacgcc gcgcatcaca gcatgccgga ccgtggcagg cagatcgctg cgctcgttca
     9841 gcgcctcacg cagcgatgcc tcgccggggt agcgttcgat catggcggca aagctcgcgg
     9901 cggcgatttc gccgcccgga ttgcgcaaca gactgatcag cgccgcgggc tccgcagttg
     9961 ccgccagcac cgccgatact ctcgccggca ccagcggccg caaggcgatg gcggtctgcg
    10021 tcagaaccgt tccgtttgcc gcacagtcaa tcagatccgc ttcgcgcagc aggggggacc
    10081 gggcaagcac cagcgccgcg acctccggct gatcatgcgc cagcgcgacg atgatgtggc
    10141 gtggcgcgga ctcgcaggcc gcgagttccc aggccaacgc acggcgcacc tgcatcgacg
    10201 ggtcatcgag gatcagcgtc agcgccgcct cggcatcctc gcgactgtcg gaacccagat
    10261 ggcctgcgac ataggcctgc gtcatgccac gcgctgcctc gatccgttcc tcgacgggcg
    10321 cattctcgaa ccatttcagg aagctacgaa cgatcacgag acacgactcc ggacgcgggc
    10381 aaatgccatg aggtcaagcg gggcgccgat cgccccaggt cgcgccgcat cgcgcgcggt
    10441 gccagtc
//
LOCUS       DAOMXN010000071        10383 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_3426_length_10383_cov_12.698683, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000071 DAOMXN010000000
VERSION     DAOMXN010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 10383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10383
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3426_length_10383_cov_12.698683"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..224)
                     /locus_tag="PK812_06890"
     CDS             complement(60..224)
                     /locus_tag="PK812_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007827291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HRJ69318.1"
                     /translation="MKTLFARFAKDESGATAIEYGLIASLVAVAIITALTTLGTKLTA
                     TFGSVSNKLK"
     gene            761..1180
                     /locus_tag="PK812_06895"
     CDS             761..1180
                     /locus_tag="PK812_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003598130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HRJ69319.1"
                     /translation="MSTPARAMDSIEVHMDNARLLKVPEGTDTIVVGNPLIADVTIQR
                     NQVMVVTGKNFGTTNMIALDGKGAIISESQIIVSTPHKDRLTVVRGFDLETYACNPNC
                     GPVMQIGDSDRHFGRAGAQSRDRSQTATSGAGAAPGR"
     gene            1342..1965
                     /locus_tag="PK812_06900"
     CDS             1342..1965
                     /locus_tag="PK812_06900"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HRJ69320.1"
                     /translation="MIAPLIGRLLRRLRPVEADGSVPDAIRSGAVFRFRRSRDGATAV
                     EFGLIAMPFFMLLFAIIETGMIFFTSGVVDKAVYNAGRQIMVGAVARTPGPPAAKLTK
                     FKTDLCNQLSWFVDCNDIVLDVQSFKTYGDVDPSIPVRNGDLDMASLPRFNPGQAGEI
                     VLVRAYYPVKVYTSFLDGLPNLNGNRRLIMGVYSFKNEPFGKTGTGV"
     gene            1962..2603
                     /locus_tag="PK812_06905"
     CDS             1962..2603
                     /locus_tag="PK812_06905"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HRJ69321.1"
                     /translation="MNGETLKTRPMPSMNRALRRRFHALLKRRDGIAAVEFALVLPIM
                     VAMYFGLVELTAGFGHDARAARLAQALSDMVTQSTAINHTDMNTIFMSATTVLQPYQA
                     TGVAMRVTSYVIDRNGNAFVDWSDVRNISPKTPFGPRPRCFNANAILPAWLRLPQTSV
                     VYSEVAFVYQPVIGQFVAPTGLEMKSSHPTSPRGGTPVGREGTPATNCPGYAP"
     gene            2797..3411
                     /locus_tag="PK812_06910"
     CDS             2797..3411
                     /locus_tag="PK812_06910"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRJ69322.1"
                     /translation="MSKVRNVVFDVGNILLRWDPRNLYRKIFSDPAAMEQFLETICTS
                     EWNLQGDMGRSWRDLTEELVVAHPAHEANIRAYRSRWHEMVIGSIDENVRLLETFKRN
                     GVPLYAITNFSADCFAESQERFPFLKTFDGIICSGVERVVKPDPAIYRLLLDRYGLQA
                     AECVFIDDSEKNIIAASKIGFKTIHYGLGVDSRLAFAQFGLPVD"
     gene            complement(3424..3963)
                     /locus_tag="PK812_06915"
     CDS             complement(3424..3963)
                     /locus_tag="PK812_06915"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HRJ69323.1"
                     /translation="MMKLLGSVLAMGGLTAAVFVFWLGRFDLSAAPAQNAPAKPAQQA
                     ASTPPQALAAQAGPQSVSLAADRSGHFVTDVRINGIFIKGLVDTGATIIAIPLKDART
                     LGINPPQSAYTMPIRTANGEVRAAKVTLGEVRIGPVVVAGVDAAVVPQGLDSTLIGMS
                     FFRRLSSFEMRGQSLVLRQ"
     gene            complement(4118..5749)
                     /locus_tag="PK812_06920"
     CDS             complement(4118..5749)
                     /locus_tag="PK812_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020179579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ69324.1"
                     /translation="MSEAPLLQVRDLSVAFTQVGRTTLAVDRISFDLKRGETLALVGE
                     SGSGKSVSALSIVRLLSYPAASHPSGDIQFDGQDMLGADEAAMRKVRGNKITMIFQEP
                     MTSLNPLHTVERQIGEILLVHQGLAGAAARAKVLELLTKVGIPKPETRLESYPHQLSG
                     GQRQRVMIAMALANEPDLLIADEPTTALDVTVQAQILDLMQQLQRETGMAMLFITHDL
                     GIVRRVAHNVCVMRKGKVVESGPVAEVFARPQHEYTRALMDAEPKGLPPPEDLTRPII
                     LKADDIKVWFPVKRGLLRRTVDHIKAVDGISFEVRAGQTVGVVGESGSGKTTLGLAAL
                     RLIRSQGPVVFLGTRIDGLGFSDLRPLRKDMQVVFQDPYGSLSPRLSVAEIVEEGLTI
                     QSPDMTATSRRDTVARALGEVGIDPAAMDRYPHEFSGGQRQRIAIARAMALSPRFVVL
                     DEPTSALDMSVQAQIVALLKKLQAERDLAYLFISHDLKVVRALANHLIVMRHGKVVEQ
                     GPAADVFSAPTSDYTRALLQAAFEIRASSPNAAAR"
     gene            complement(5746..6894)
                     /locus_tag="PK812_06925"
     CDS             complement(5746..6894)
                     /locus_tag="PK812_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ69325.1"
                     /translation="MGPPLLAADEAFAAAPPTRGRLSPINQRRLANFKRNRRGLWAFW
                     IFMVLFIGSLFAEFIANDRPIIARYKGETLFPFAVNYPEEKFGGFLARTDYRDPVIAK
                     EIEENGWMLFPPIRFSYSTINRDLPAPAPSPPTWLLTDAQCKAAAAKFGGTSCRDIEW
                     NWLGTDDRGRDVVARLIYGFRISVLFGLTLALSASVIGIAAGAVQGFFGGWIDLALQR
                     FIEIWTSIPRLYLLIIISSVVAPGFLVLLVILLLFEWTALVGVVRAEFLRARNFEYVN
                     AARALGLSNGRIMVKHVLPNAMVATLTLLPFVLNGSITTLTSLDFLGFGLPPGSPSLG
                     ELLQQGKSNLQAPWLGLSGFIVIALMLSLLNFIGEAVRDAFDPRKTFT"
     gene            complement(6894..7994)
                     /locus_tag="PK812_06930"
     CDS             complement(6894..7994)
                     /locus_tag="PK812_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HRJ69326.1"
                     /translation="MFAYILRRLLLMVPTLFGILLVAFTIVQFAPGGPVERVLAQLQG
                     ADTSASSRVGGGTGDVGGQAAMQAAQGNSNYRGAQGLDPAFIKELEKQFGFDKPAHER
                     FFKMVGDYLRFEFGRSYFRDASVLDLIKEKLPVSMSLGLWMLLISYGISIPLGIRKAV
                     RDGSPFDIWTSALIVVGYAMPGFLVAVFLIVLFAGGSFFQIFPLRGLTSGNWESLSLG
                     GKVLDYLWHIVLPITAMALSAFATLTFLTKNSFLDEIRKQYVLTARMKGLDEKRVLYG
                     HVFRNAMLIVISGFPAAFVHAFFSGSILIETIFSLDGLGLLSFESILNRDYPVVFANL
                     FIFALVGLVVNLITDLTYTFVDPRIDFEAREV"
     gene            complement(8001..9893)
                     /locus_tag="PK812_06935"
     CDS             complement(8001..9893)
                     /locus_tag="PK812_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010687134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRJ69327.1"
                     /translation="MTVTDKVHRPDRRSLLAGAGAFVGVSTLLPSGTWAQQAASFGPE
                     RHGLSIFGDLKYPPGFAHFDYVNPSAPKGGEMALQVSSVGGNQNFLTFDTLNMFSSKG
                     SGAAGLGLIFDSLMSSAADEPDSMYGLVARSVQASADGLTYRFHLRPEARFQDGTPLT
                     AEDVAFSLTLLKSKGYPTYRLILKDLVSAEARAADIVDIRFAPGRSREMPLIVAGMPI
                     LSKTYYETKDFEAATLEQPLASGPYKVLKADAGRSVTYQRDPNYWGAKLAVHVGTSNF
                     ERVRYEYFRDREAAFQGFTSGTFTYREEFTSLIWATRYTFPAFRDGRVKREVIEDHTP
                     SGTQGWFLNTRRPQFKDRRIREAVGLAFDFEWSNKNLMYDSYRRTVSYFENSTLKATG
                     RPSPEELALLEPFRGKVPDEVFGEPFVPPVSDGSGQDRTILRRAQQLLQEAGCKRDGG
                     GRLSLPDGKPFTIEFMEDEPSLNRHMEGFVKNLKLLGIEASIRVVDATQFQRRVEDFD
                     FDLISRRWNMGETPGENLKVLFHSDNANARASRNLSGVADPVVDALVQKAIDAKSRAE
                     LVVACQALDRVLRAGRYWVSAWFKGSHWVAYWDMFDRPKEQPRYGFNPVGTWWHDAEK
                     AKRIGL"
     gene            complement(10020..>10383)
                     /locus_tag="PK812_06940"
     CDS             complement(10020..>10383)
                     /locus_tag="PK812_06940"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HRJ69328.1"
                     /translation="AAEPIAVRLAKADAKKGEAAAKQCLSCHKLEKGGANGQGPALYG
                     VVGKDAGKAAGFAYSPAVAGLGKKWDFELLDAFLANPKTAVPGNKMTFAGISRPDTRA
                     DLIAFLNGLSDSPLPLPK"
BASE COUNT         1991 a         3189 c         3323 g         1880 t
ORIGIN      
        1 aaacgaaagc ggtccgctcg ttgccgagcg gaccgctttg agaaaactcg ggttcaagct
       61 tacttcagct tgttgctcac cgaaccgaag gtcgcggtca gcttggtgcc cagcgtggtc
      121 agcgcggtga tgatggcgac ggcgaccagc gaggcgatca ggccgtactc gatggcggtg
      181 gcgccggact catccttggc gaaacgggcg aaaagggtct tcatagctag ctcctgaatc
      241 acttgttgct ccagtcacga tggcgacttc tcatccgctc atcgaaacta ggggcatcct
      301 aggccggacc tcttgaagac ttcttaatgt cacagcagaa tcgagaaaga atttaagtcg
      361 ggcactttgt ggttaagaaa cgcatattgt tcatttgtaa aacaacagat acatatgatt
      421 tgcatgatgc atgcttgtat tgtgcaatga ttatctgtgc taatgtgtga ttaatgctag
      481 tttagatggg actttttacg gaagaatgtt ggttttgttc tggcgggccc gcggcatctc
      541 cagcgtcagg cgagaggctg gtgacgtcaa aatccagatt tagcgatttg ttcaccaaga
      601 cagaccacta tcgtaagcca gttcgcaggc cgatgccgcc tgcaagacgg ttatccggag
      661 aattttcatg gccgccacgt gcaaccagcc tgtttccgcc cgcctgaaaa atggcctgcg
      721 ggccgcactg gcaattggac tgctcgcagc aggagccgcc atgagcacgc ccgcgcgcgc
      781 gatggactct atagaagtcc acatggacaa tgcccgcctg ctgaaggttc cggaagggac
      841 cgacacgatc gttgtcggca atccgctgat tgccgacgtc acgatccagc gcaatcaggt
      901 catggtggtc acgggcaaga atttcggcac aaccaacatg attgccctgg atggcaaggg
      961 cgccatcatt tcggaatcgc agatcatcgt gtcgacgccg cacaaggaca ggctgacggt
     1021 ggtgcgcggg ttcgatctcg aaacctatgc ctgcaacccg aattgcggac cggtcatgca
     1081 gatcggcgac agcgaccggc acttcggtcg ggccggggca cagtcgcggg atcgcagcca
     1141 gaccgcgacc agcggcgcgg gcgctgcgcc tggtcgctga tcggccggac ataacctttt
     1201 cctaaccgta acgtcctttg aagcatgcgg atcgcagact ttgcgctgcg ggaaatcatg
     1261 tagcaacttt ccctgcgtta aatccgcttt gattgtcgca agggcctttg tggtctcgaa
     1321 gtggttgcgg cggagggaac catgattgcg cccctgattg gacggctatt gcgaaggctg
     1381 cggcctgttg aagccgacgg gagcgtcccc gacgcgatca ggtctggggc cgtgttccgt
     1441 ttccggcgct ctcgggatgg tgcgaccgcc gtcgaatttg gcctgatcgc catgcccttt
     1501 ttcatgctgc tgttcgcgat catcgagacc ggcatgattt ttttcacgtc gggcgtggtg
     1561 gacaaggccg tctacaatgc cggccggcag atcatggttg gcgccgtcgc acggactcca
     1621 ggcccgccag cggcaaaact cacgaaattc aagacagacc tgtgcaacca actgtcctgg
     1681 ttcgtggact gcaacgacat tgttctggat gtccagtcat tcaagaccta cggcgatgtc
     1741 gatccgagca tcccggtcag gaatggcgat ctcgacatgg cttctctgcc caggttcaac
     1801 cctggccagg ccggtgaaat cgtcctggtg cgggcctact atccggtcaa ggtctacacg
     1861 tcgttcctag atggcctgcc caatctgaac ggcaaccgcc ggctgatcat gggtgtgtat
     1921 tctttcaaga acgagccgtt cggcaagacg gggaccggcg tatgaacggt gaaacactga
     1981 agacgcggcc catgccatcg atgaacaggg ccctgcgccg gcgctttcat gccctgctga
     2041 agcgccgcga tggaatcgca gccgttgaat tcgccctggt gctgccgatc atggtcgcga
     2101 tgtatttcgg tctggtcgag ctgacagcgg gttttggtca cgatgcacgc gccgcgcggc
     2161 ttgcgcaggc cctgtcggac atggtcaccc agtccaccgc gatcaatcat accgacatga
     2221 acacgatctt catgtcggcg acgaccgtcc tgcagcccta tcaggcgacc ggtgtggcga
     2281 tgcgcgtgac aagctacgtg atcgatcgca atggcaacgc tttcgttgac tggtcggatg
     2341 tcaggaacat ctcgcccaag acgccctttg gaccgcgtcc gcgctgcttc aacgctaacg
     2401 ctatcctgcc ggcatggctt cgtcttcccc agacttccgt ggtctactcg gaagtcgcgt
     2461 ttgtctatca gccggtcatc ggtcagttcg tggctccgac aggcttggag atgaagtcca
     2521 gtcaccccac cagcccgcgc ggcggcacgc cagtggggcg tgaaggaacg ccggcgacga
     2581 attgccctgg ctatgcgccg tgatgacagg tcgcgaggcg tcactgtcct cgcgcaaggg
     2641 tccgcctgaa ggtcccgcgt cacgtcaggg acttgagcag ccatagcgtg ttgcggaaga
     2701 cacggggcag gcgtgccgcc ggccatggaa cggcttgccg gatgcggcgc tgactgacag
     2761 cctccttgtc caagagaatc agagggaaga gacgctgtgt ccaaggtccg caatgtcgtt
     2821 ttcgatgtcg gcaacatcct gctgcgatgg gatccccgca atctttaccg caagatcttc
     2881 tccgaccctg ccgcgatgga acagttcctg gaaacgatct gtacgtctga atggaacctc
     2941 cagggagaca tgggacgatc ctggcgcgac ctgaccgagg aactggtggt cgcccatcct
     3001 gcccatgagg ccaatatccg cgcctatcga tcacgctggc atgagatggt gatcggatcg
     3061 atcgacgaga acgtgcggct gctggagacg ttcaagcgga atggggtgcc gctctacgcc
     3121 atcaccaatt tctcggccga ctgcttcgct gaatctcagg aacggttccc cttcctgaag
     3181 acgttcgatg gcatcatctg ctccggcgtt gagcgagtcg taaaaccgga tcctgcgatc
     3241 taccggctgc tgctggacag gtacggcctg caagctgcgg aatgcgtgtt catcgacgac
     3301 agtgagaaga acatcattgc ggccagcaag attggtttca agaccatcca ctacggtctg
     3361 ggggtcgact cccgcctggc gttcgcccag ttcggtctgc cggtcgattg agcggcgtct
     3421 ggtctactgg cgcagcacca gggactggcc gcgcatctcg aaactggaca ggcgcctgaa
     3481 gaagctcatg ccgatcagcg tgctgtcgag cccctggggc acgacggcgg cgtcaacgcc
     3541 ggcgacgacc accggtccga tgcgaacctc gcctagggtg acctttgccg cccgcacctc
     3601 gccgttcgcg gtgcggatcg gcatcgtgta ggcgctctgc ggcgggttga tgccaagcgt
     3661 ccgcgcgtcc ttcagcggaa tggcgatgat ggtcgcgccg gtgtccacca accccttgat
     3721 gaaaatgccg ttgatgcgca cgtccgtcac gaagtgaccg gatcggtcgg cagccagaga
     3781 aacggattgc ggtcctgcct gagcggctag ggcttgcggc ggcgtcgagg ctgcctgttg
     3841 agctggtttc gccggcgcgt tctgggccgg agcggcgctg agatcgaagc gtccgagcca
     3901 gaagacgaag acggcggcgg taagtccgcc catcgccagc accgatccca gaagcttcat
     3961 catgacgcgg ttcgccctgt ggtggagcgg acaccatagg cagcgaaggt tgatcagcgg
     4021 tgtgcgcggc gccccggggc gcgggcgtgg ttgacgggac gtaaatgcgg tgcgcaaggg
     4081 gctcgccgct cgagcgcggc gggatggcgc acgtgattca acgcgccgcc gcgttagggg
     4141 aactggccct gatttcgaat gcggcctgca gcagggcgcg cgtgtagtcg ctcgttggcg
     4201 ccgagaagac atcggccgcc ggtccctgtt cgaccacctt gccatggcgc atgacaatga
     4261 ggtggttggc cagggcacgc accaccttga ggtcgtgcga gatgaacaga taggcgaggt
     4321 cacgctcggc ttgcagcttt ttcagcaggg cgacgatctg cgcctgcacg gacatgtcca
     4381 gcgccgaagt cggctcgtcc agcacgacga aacggggcga cagcgccatg gcgcgcgcga
     4441 tggcaatgcg ctggcgctga ccgccggaaa actcgtgtgg atagcgatcc atcgccgccg
     4501 gatcgatgcc gacctcgccc agagcacgtg ccacggtgtc gcgccggctg gtcgcggtca
     4561 tgtccggtga ttggatcgtc aggccttctt ccacgatttc cgccaccgac aggcgcggcg
     4621 acagcgaccc gtaagggtcc tggaagacaa cctgcatgtc cttgcgcagc gggcgcaggt
     4681 ccgaaaagcc gaggccatcg atgcgggtgc cgaggaaaac gacgggtccc tgcgagcgga
     4741 tgagacgcag cgccgccaac cccaatgtcg tcttgcccga cccggactcc ccgacgacgc
     4801 ccaccgtctg tccggcgcgc acctcgaagg agatgccgtc caccgccttg atgtggtcga
     4861 cggtgcggcg aagcaggccg cgcttgaccg ggaaccagac cttgatgtcg tccgccttca
     4921 ggataatcgg cctggtaagg tcctccggcg gcggcaggcc cttcggttcg gcgtccatca
     4981 gcgcgcgggt gtactcgtgc tgcggccggg caaaaacctc ggccaccggc cctgattcca
     5041 ccaccttgcc cttgcgcatg acgcagacgt tgtgcgccac ccgccgcacg atgccgagat
     5101 cgtgggtgat gaacagcatc gccatgcccg tttcgcgctg aagctgctgc atcaggtcga
     5161 ggatctgcgc ctgcacggtg acgtcgagcg ctgttgtcgg ctcgtcggcg atcagcaggt
     5221 ccggctcgtt ggcgagcgcc atggcgatca tcacgcgctg acgctgtccg ccagaaagct
     5281 gatgcggata ggattcaagc cgcgtctccg gcttcggaat gccgaccttg gtcagcagct
     5341 ccagcacctt cgcgcgcgcc gccgctcccg caagcccctg atgcaccagg aggatttcgc
     5401 caatctgccg ctccaccgta tgcagcgggt tgagcgaggt catcggctcc tggaagatca
     5461 tggtgatctt gttgccgcgc accttgcgca tcgcggcctc gtccgccccg agcatgtcct
     5521 gcccgtcgaa ctggatgtcg ccggacgggt gcgatgcggc gggataggac agcagccgca
     5581 cgatggagag cgccgagacc gacttgccgg acccggattc gcccaccagc gccagcgtct
     5641 cgccgcgctt gaggtcgaac gagatgcgat cgaccgccag cgtggtgcgc ccgacctgcg
     5701 tgaaggcgac ggaaaggtcg cggacctgga gcagcggggc ctcgctcatg tgaaggtctt
     5761 ccgcggatcg aaggcgtcac gcaccgcttc gccgatgaag ttcagcagcg acagcatcag
     5821 cgcgatgacg atgaagccgg aaagtccgag ccagggcgcc tgcaggtttg acttgccctg
     5881 ttgcagcaat tcgccgagcg agggcgagcc gggcggcagg ccgaaaccca ggaagtcgag
     5941 cgatgtcagc gtggtgatcg agccattgag gacgaagggc aacagcgtca gtgtggcgac
     6001 catggcattg ggcaacacgt gcttcaccat gatgcgtccg ttggagaggc caagcgcacg
     6061 cgcggcgttg acgtactcga aattgcgggc gcgcaggaac tctgcccgca ccacaccgac
     6121 cagggccgtc cattcgaaca acaggagaat gaccagcagg accaagaatc ccggggcgac
     6181 cacggatgat atgatgatca gcaggtagag ccgcggaatc gacgtccaga tctcgatgaa
     6241 ccgttgcagc gcaaggtcga tccagccgcc gaagaatcct tgcacggccc ctgccgcgat
     6301 gccgatgacg gaggccgaga gcgccagggt caggccaaaa agcacggaga tgcggaagcc
     6361 gtagatcagc cgcgccacca cgtcgcggcc gcggtcgtcg gtgcccagcc agttccattc
     6421 gatgtcgcgg caggacgtgc cgccgaactt cgccgccgcg gccttgcatt gcgcgtcggt
     6481 caggagccac gtcggcggcg agggagccgg ggccggcagg tcgcggttga tcgtgctgta
     6541 ggagaaccgg atcggtggaa acagcatcca gccgttctcc tcgatctcct tggcgatgac
     6601 cggatcgcgg tagtcggttc gggccaggaa gccgccgaac ttctcttccg ggtagttcac
     6661 cgcgaaggga aacagcgttt cgcccttgta gcgggcaatg atgggtcggt cgttggcgat
     6721 gaactcggcg aacaggctgc cgataaacag caccatgaag atccagaacg cccagagccc
     6781 gcgccggttg cgcttgaaat tcgccagtcg gcgctggttg atcggtgaca gccgcccgcg
     6841 cgtcggcggt gccgcggcga aggcctcgtc agcggcgagg agggggggac ccatcacacc
     6901 tccctggcct cgaagtcgat ccgcggatcg acgaaggtgt aggtgaggtc ggtgatgaga
     6961 ttcaccacca gtccgaccag cgcgaagatg aacaggttgg cgaacaccac cggatagtcg
     7021 cggtttagga tcgattcgaa tgacagcagg cccaggccgt cgagcgagaa gatcgtttcg
     7081 atcaggatcg agccggaaaa gaaggcgtgc acgaaggcgg ccggaaatcc ggaaatcacg
     7141 atcagcatgg cgttacggaa cacatggcca tagagcacgc gcttttcatc gagccctttc
     7201 atgcgggccg tgagcacgta ctgcttgcgg atctcgtcca ggaaggagtt cttggtgagg
     7261 aatgtcagtg tcgcgaaagc ggaaagcgcc atggccgtga tcggcagcac gatgtgccag
     7321 agataatcca ggaccttgcc gccaaggctc aggctctccc agttgcccga cgtcaggccg
     7381 cgcagcggga aaatctggaa gaacgacccg ccggcgaaca gcacgatcag gaacaccgcg
     7441 accagaaatc ccggcatcgc atagcccacg acgatcaggg cggatgtcca gatatcgaag
     7501 ggagacccgt cccgcaccgc cttgcggatg cccaacggta tggaaatgcc gtaggagatc
     7561 agcaacatcc acagaccaag gctcatcgag accggcagtt tctccttgat caggtccagc
     7621 accgaggcgt cgcggaaata gctgcgacca aactcgaaac gcaggtaatc gcccaccatc
     7681 ttgaagaagc gctcatgcgc cggcttgtcg aagccgaact gcttttccag ctccttgatg
     7741 aaggcaggat cgagcccctg cgcgccgcgg tagttggaat tgccctgcgc cgcctgcatc
     7801 gctgcctgtc cgccgacgtc gcccgtgccg ccaccgacgc gggaggacgc gctcgtgtcg
     7861 gcgccctgca actgcgccag cacgcgctcc acaggcccgc cgggcgcgaa ctgcacgatc
     7921 gtgaacgcga ccagcaggat gccgaacagc gtcggcacca tcaggagcaa gcggcggagt
     7981 atgtaggcga acatggagtc ttataggccg attcgcttgg ccttttctgc gtcgtgccac
     8041 caggttccga ccggattgaa gccgtagcgc ggctgctctt tcggccggtc gaacatgtcc
     8101 cagtaggcaa cccagtgcga acccttgaac caggccgaaa cccagtagcg cccggcgcgc
     8161 agaacccggt ccagcgcctg gcaggcgacg acaagctcgg cgcgcgactt ggcgtcgatc
     8221 gctttctgca ccaatgcatc caccaccgga tcggccacgc ccgacagatt gcgcgaggcg
     8281 cgcgcgttgg cgttgtcgga atggaacagc accttgaggt tctcgcccgg cgtctcgccc
     8341 atgttccagc ggcgggagat caggtcgaag tcgaaatcct cgaccctgcg ctggaactgc
     8401 gtcgcgtcca ccacccgaat ggacgcttcg atgcccagca gcttcaggtt cttgacgaat
     8461 ccctccatgt gccggttgag ggatggctca tcttccatga attcgatggt gaatggtttt
     8521 ccgtccggca gggagagccg cccgccgcca tcgcgcttgc acccggcttc ctgcagcagc
     8581 tgctgcgccc ggcgcaggat ggtccgatcc tgccctgatc cgtccgaaac gggcggaacg
     8641 aaaggttcgc cgaacacctc gtccggcacc ttgcccctga agggctccag cagggccagt
     8701 tcctcgggcg aggggcgacc cgtcgccttc agcgtcgaat tctcgaaata ggaaaccgtc
     8761 cgccggtagg aatcatacat caggttcttg ttcgaccatt cgaaatcgaa ggcgagcccc
     8821 acggcctcgc ggatgcggcg atccttgaac tgcggccggc gcgtgttcag gaaccagcct
     8881 tgcgtgcccg agggcgtgtg gtcctcgatc acctcgcgct tgaccctgcc atcccggaag
     8941 gccgggaagg tgtagcgtgt cgcccagatc aggctggtga attcctcgcg ataggtgaag
     9001 gtgccggaag tgaacccctg gaaggccgct tcccgatccc ggaaatactc atagcgcacg
     9061 cgctcgaaat tgctggtgcc gacatggacg gcgagctttg cgccccagta gttggggtcg
     9121 cgctggtacg tcacggaacg cccggcatcc gccttgagca ccttgtaggg tcccgaggcg
     9181 agtggctgtt ccagcgtcgc agcctcgaaa tccttggttt cgtaatacgt cttcgacagg
     9241 atcggcatgc ccgcgacgat cagcggcatt tcccggctgc ggcctggcgc gaagcggatg
     9301 tcgacgatgt cagccgctcg ggcctcggct gagacgagat ccttcaggat cagccgatag
     9361 gtcggatagc ctttgctctt caggagggtg agcgaaaagg cgacgtcctc cgccgtcagc
     9421 ggcgttccat cctggaagcg cgcctcgggc ctgaggtgaa accggtaggt caggccatcc
     9481 gcactggcct gcaccgaacg ggccacgagg ccgtacatcg aatccggctc gtcggccgcc
     9541 gaggacatca ggctgtcgaa gatcaggccg agcccggcgg cgccgctgcc cttggacgag
     9601 aacatgttca gcgtgtcgaa ggtgaggaaa ttctggtttc cgcccaccga cgagacctga
     9661 agcgccattt cgccgccctt cggggcgctc gggttgacgt agtcgaaatg cgcgaaacct
     9721 ggcgggtatt tcaggtcgcc gaatatcgaa agtccgtgcc gttccgggcc gaagcttgcg
     9781 gcctgctgcg cccaggtgcc ggatggcagc agggtggaaa cgccgacgaa agccccggcg
     9841 ccggccagca ggctgcgccg atccgggcga tgaaccttgt cagtcaccgt catcatctct
     9901 tccctgtgcc gaaaccgccc aaatcacttg gcgcggatta tgcctgcatc aaggccgctg
     9961 gccagaaaag aaaaggccgg gttgccccgg cctcgaaacc tgtcagagag ccgcggcgct
    10021 cacttcggca gcggcagcgg gctgtcggag aggccgttga gaaaagcgat caggtcggcg
    10081 cgcgtatccg ggcgggagat gccggcgaac gtcatcttgt tccccggaac cgccgtcttc
    10141 gggttggcga ggaaggcatc gagcagttcg aagtcccact tcttgcccag gccagcgacg
    10201 gccggcgaat aggcaaagcc ggccgccttg ccggcatcct tgccgacgac gccgtagagg
    10261 gcggggccct gaccgttggc gccgcccttt tccagcttgt ggcaggagag gcactgcttg
    10321 gcggcggcct cgcccttctt ggcgtcagcc ttggccagac gaacggcgat tggctccgcc
    10381 gca
//
LOCUS       DAOMXN010000072        10351 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_3439_length_10351_cov_11.388889, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000072 DAOMXN010000000
VERSION     DAOMXN010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 10351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10351
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3439_length_10351_cov_11.388889"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1352
                     /gene="mutL"
                     /locus_tag="PK812_06945"
     CDS             <1..1352
                     /gene="mutL"
                     /locus_tag="PK812_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HRJ69329.1"
                     /translation="PARLKFLKTDRAEAMAVAQMLRRLAMAQPHVRFTLAGDNLSGFD
                     HAACGSGPAARLKRLSQVLGTDFRDNALAIDAEREGVRLEGFVGLPTFSRATNAEQYL
                     FVNGRPVRDKLLHGAVRAAYQDTLPADRHAVVALFLELDPREVDVNVHPAKAEVRFRD
                     PGLVRGLIVGALRHALSQAGHRASTTGTIGLQTAFRHPTHAHHAAGSGTRAAHDWRTS
                     PFAPSPAAEPPQAGFDIDLAPTVRAGAMGHPDAGDHTIDHPLGAARAQIHATYIVSQT
                     RDGFLLIDQHAAHERLVYERLKRGREGNAAPRQMLLIPLVVDLGEAEAMRLADSAAAL
                     ERLGLVLEPFGPGAVVVREVPAPLAGGDTAALVRDLADHIGEWGGPEKLAGITDLVLK
                     TFACHHSVRAGRQLRLEEMNALLREMEATPGSGQCNHGRPTWIELKLGDIERLFGRS"
     gene            complement(1356..3374)
                     /locus_tag="PK812_06950"
     CDS             complement(1356..3374)
                     /locus_tag="PK812_06950"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRJ69330.1"
                     /translation="MDGTPLAGAWSAASFTAIGAAVLALAACVAMIRDVARLRLRKQV
                     LRTRLQEHEAKLDETRETVSGLKALIEKSGDLYVKLDSNGIILEGSAAFNALWARHAD
                     GAKVYFEAFGTLKGTRREGSVETVLADRTIRWTFTTIGRGEGSSVVAVGRDMTAGGDS
                     ETAVGAKSRFLATVSHEMRTPLNGVLGMVGLLRDTGLSPEQENYASAIDASGEALLSL
                     INEILDFSKIEAGKLEIASETVAIETLVEGVVELLSPRAQDKGIEIAGFVHRAVPAKI
                     VSDGARLRQVVMNLAGNAVKFTEKGGVGIRVETTTDSILAITVADTGIGIPADRLAAI
                     FEDFEQADTGVAGKHGGTGLGLAISRRIVERLGGAISVMSRVGAGSAFRITFPLVPAD
                     GADAPHKAPQVLAGRSVLIVSPSPFEAPFLAERLEDLGARARLIASVSDAQGAIRDVD
                     DLIVDAALGHKACVALAEAAGGGGVQRRLILLSPYERRGFGSPAEAGFDGYLVKPVRQ
                     RSLAARFVESSAAEVDDLPKAAPCERDATPLQGARVLIAEDNPINALLARRLVEKLGA
                     IAVWAKDGVEAQQVLLGEGTQRFTLALFDWRMPGLDGLQAVTWLRAHEAAFNLPRLPV
                     AALTANAFEEDRQECLAAGFDAFLPKPLERESFLTVVRALVRPERQAA"
     gene            complement(3504..5024)
                     /locus_tag="PK812_06955"
     CDS             complement(3504..5024)
                     /locus_tag="PK812_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HRJ69331.1"
                     /translation="MVTRVATVAFEGIEARPVDVQVQVASGNVAFVLVGLPDKAVAES
                     KERVRAALTASGLAMPAKRVVVNLAPADLPKEGSHFDLPIALGIMAAIGALPSDALDG
                     FTVLGELALDGAISAVAGVLPAAVAANARGHGLICPKSCGPEAAWAGGDMDVLAPASL
                     IQLANHFKGTQVMARPHPAVRSGEGVLPDLRDVKGQENAKRALEIAAAGGHNLLMSGP
                     PGAGKSMLAQRLPSILPPLGPRELLEVSMIHSVAGLLTGGALTDRRPFRSPHHSASIA
                     ALTGGGLHARPGEVSLSHHGVLFLDELPEFQPQALDSLRQPLESGEVSVSRANHRVTY
                     PARFQLVAAMNPCKCGRATEPGYACKRAPNEKCMAQYQARLSGPLIDRFDLMIEVAAV
                     TAADLVLPPPSEGSAQVAARVAAARLRQAERYAAMGLATGTNATTSAAAIEEVARPDD
                     AGAKLLRDASEKFRLTARGFHRVMKVARTIADLDGEIRVSRIHLAEALSYRVMPMA"
     gene            complement(5094..6065)
                     /locus_tag="PK812_06960"
     CDS             complement(5094..6065)
                     /locus_tag="PK812_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HRJ69332.1"
                     /translation="MRALRELTEREVLALAIANEEEDARIYQVFAEKCRSDFPASAKI
                     FDDMAEEERDHRASLFAAYRNRFGEHLPPIRREDVKGFLKRRSVWWTKNLSPDTMRRE
                     AAIMELQAGNFYDKAATQTLDTSVRELYLHLAEVERGHEKRAAELAEAHLTDSAQDEE
                     AATKHRLFVLQYVQPGLAGLIDGSVSTLAPLFAAAFATHSNHQTFLVGLAASVGAGIS
                     MGLTEALSDDGIITGRGSPWMRGLVCGMMTMIGGLGHTIPYLVPDSWPNGFYIATAMA
                     AVIVAIELLAISWIRTKYMESPFWQATLQVIVGGVLVLLSGILIGSS"
     gene            6164..6946
                     /locus_tag="PK812_06965"
     CDS             6164..6946
                     /locus_tag="PK812_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69333.1"
                     /translation="MTASLLALALKMGLVAAIVVGAASIAERSRPAVAALVATLPVSI
                     GPTYVLLALDHDAAFLTAAALKSAASVGATMAFIMAYANALTRFGPASALAAAYCAWI
                     PAAWFVHHHAWTVGEVVMFVALTSIAGAIAMRRLRHHQAESSGQRRRWDVPARAAGVV
                     ALVGAITALSESLGPSGSGTLANFPVVMTSVTLITHLRLGGLAAAAMLANSTGGMVGV
                     SAALLTVHFAIPVLGAALALLTGLVFCIVWNLGIFLRAHHRF"
     gene            7044..8006
                     /locus_tag="PK812_06970"
     CDS             7044..8006
                     /locus_tag="PK812_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042918 - alkanesulfonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HRJ69334.1"
                     /translation="MKLSRRGFLATAASVALARPALAQKLSLITLDWATYNPLSLIIR
                     DNKWLEEDFAKESVAIRWVQSAGSNKALEFLNASSIDFGSTAGAAALLGRIGGNPIRS
                     VAVYSRPEWTALVTKGDKVKTVADLKGKSVAVTRGTDPHIFLIRALLDHGVAASDVKF
                     VLLQHADGKTALVRGDVDAWAGLDPIMAQAEVEDGARLFYRKPENNTWGILNAREDFA
                     AKNPEIVKRVIAVYEKARKHALANPKLVVEALVKAARLPDTVIAKQIERTDFGTSVIG
                     PVQAEAIEQAGLALQKAEVIKGDVNVKATVAALIDASFTSGLKS"
     gene            8026..8868
                     /locus_tag="PK812_06975"
     CDS             8026..8868
                     /locus_tag="PK812_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ69335.1"
                     /translation="MTANDLALQQAGEAASGSEGQPAAAGWLKGLAVGVALPALLALG
                     WEVAVRAGVSNGRLVPPPSRILATLVDLWRTGDLVMHIVATLRRVALGFAFGAVAGTI
                     LGGISGASAMFRRLTDPMFQGFRSVPSIAWVPLFVLWFGIFEAPKVMLIAVGVFFPVY
                     LGVLGAIQSVDRKLVEVGQVFRLSQAQMLRRILLPAILPSWAVALRSGLGLGFMFVVA
                     AELMGASEGLGYLLVDGQQLGKPDQIVAAIIVFALLGKIADAVLVRLSAPLLAWQDTA
                     RPAL"
     gene            8873..9655
                     /locus_tag="PK812_06980"
     CDS             8873..9655
                     /locus_tag="PK812_06980"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ69336.1"
                     /translation="MLTIEALTKTYAGGTTALSNVSLSMQAGEIVALVGGSGCGKTTL
                     LRLVAGLDRPSGGSVAVDGNVVTEPNAAVGLIFQEPRLLPWLSVADNIAFGLKHLPRK
                     EQRARVDFALMRVGLAGYNDRWPRQLSGGQAQRVAISRALAPEPKVLLLDEPFSALDA
                     FTRASLHDHLLALWAAQRPTMLMVTHDVEEAVTLADRVVVMQPRPGRIFAQIAIDFPR
                     PRDPLSDAFIAAKRRILEALDGSLSESRPKPKPDESPAAAWW"
     gene            9684..10196
                     /locus_tag="PK812_06985"
     CDS             9684..10196
                     /locus_tag="PK812_06985"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRJ69337.1"
                     /translation="MDAEALRTLQAPLKDRYKSDSGAARLTLKASGEIDESKIACKIE
                     TGRAIAEAGLHPLTGGTGAELCSGDMLLEALVACAGVTLKAVSTAIGVNLQRGRVTAE
                     GDLDFRGTLGVAKDAPVGFSAIRLTFDLKTDATDEQVAQLIKLTERYCVVFQTLRTSP
                     ATSVETLVER"
BASE COUNT         1724 a         3503 c         3335 g         1789 t
ORIGIN      
        1 ctccggcccg gctgaagttc ctcaagacgg atcgggccga ggcgatggcg gtcgcccaga
       61 tgctgaggcg cctcgccatg gcccaaccgc acgtgcgttt caccctggcc ggcgacaacc
      121 tgagcggctt cgaccacgcc gcctgcggca gtggccccgc agcgcgcctc aagcgactga
      181 gccaggttct gggtaccgac ttccgcgaca atgcgctggc gatcgacgcg gagcgcgaag
      241 gcgttcgcct cgaaggcttc gtcggattgc cgaccttttc ccgggcaacg aacgccgagc
      301 aatacctgtt cgtcaacggc cgaccggtcc gggacaagct gctgcatggc gccgtcaggg
      361 ccgcctatca ggataccctg ccagcggacc gtcacgccgt cgtcgccctg tttctggagc
      421 ttgacccgcg cgaggtggac gtcaacgtgc acccggccaa ggcggaagtg cgctttcgcg
      481 accctggcct ggtgcgcggg ctgatcgtcg gcgcgctccg ccatgcgctg tcgcaggccg
      541 gtcaccgcgc ctccaccacc ggcaccatcg gattgcagac ggctttccgg catccgaccc
      601 atgcccatca cgctgccggt tcgggcacgc gcgccgccca tgactggcgc acctcaccgt
      661 tcgcgccaag tccggcggcg gaaccaccgc aagctgggtt cgacatcgac cttgccccga
      721 cggtccgcgc cggcgcaatg ggtcatccgg atgccggcga ccacaccatc gatcatcctc
      781 tcggcgccgc gagggcccag atccatgcca cctacatcgt gtcgcagacc agggatgggt
      841 tcctgctgat cgaccagcat gcagcccacg agcgactggt ctacgagcga ttgaaacgcg
      901 gacgcgaagg caatgcggcg cctcgacaga tgctgctgat cccgctggtg gtcgaccttg
      961 gagaggccga ggcaatgcgg ctggcagact ccgcggccgc gctggaacgc ctgggcctgg
     1021 tccttgaacc gttcggtccc ggagccgtcg tggtgcgcga ggttcccgct ccgcttgccg
     1081 gaggcgacac tgccgcactg gtgcgcgatc tcgcggacca tatcggggaa tggggaggtc
     1141 cggagaagtt ggcggggata accgatctcg tgctgaagac cttcgcctgc caccattcgg
     1201 tgcgcgccgg acgacagttg cgcctggagg aaatgaacgc gctgctgcgc gagatggagg
     1261 caacgcccgg gtcgggccag tgcaatcacg gccggccgac ctggatcgaa ctcaagctcg
     1321 gcgacatcga acggctgttc ggccgttcct gaggatcatg cggcctgacg ctcggggcgc
     1381 acgagcgccc gcaccacggt gagaaagctc tcccgctcca agggcttggg caggaaggcg
     1441 tcgaagcccg cggcgaggca ctcctgccga tcctcctcaa aggcattggc cgtcagcgcg
     1501 gcgaccggca ggcgcggcag gttgaaggcg gcttcatgcg cgcggagcca ggtgacggcc
     1561 tgcaatccgt caaggccggg catccgccag tcgaacaggg cgagcgtgaa gcgctgcgtc
     1621 ccctccccaa gcaggacctg ctgtgcttcc actccatcct tggcccagac ggcgatcgcc
     1681 cccagcttct ccaccagccg gcgtgccagc aaggcattga tcggattgtc ttcggcgatg
     1741 agcacgcgcg cgccctgcaa cggggttgcg tcacgctcgc acggcgcggc cttcggaaga
     1801 tcatccactt cggccgccga cgattcaacg aaccgggccg ccagcgagcg ctgccgaacg
     1861 ggtttcacga gatagccatc gaaccccgcc tccgccggcg atccgaaccc acgccgttca
     1921 tagggcgaga gcagaatgag acggcgctgg acaccgccgc cgccggctgc ctccgcaagt
     1981 gcgacgcagg ccttatgccc cagagcggca tcaacgatca gatcgtcgac gtcccgtatc
     2041 gcgccttgcg catcgctgac gctcgcgatc agacgtgcgc gtgcgcccag gtcctccagc
     2101 cgctcggcca ggaagggtgc ctcgaagggc gatggggaaa cgatcagcac ggaacgcccg
     2161 gccagtacct gcggcgcctt atgcggggcg tcggccccgt ccgccggaac caggggaaac
     2221 gtgattcgga aggccgaccc ggcaccgacg cggctcatca ccgaaattgc gccgcccagt
     2281 ctttcgacga tgcgacggga gatggccagt cccaggccgg tgccgccgtg cttgccggca
     2341 acgccggtgt ccgcctgttc gaaatcctcg aaaatcgccg ccagccgatc agccgggata
     2401 ccgatgccgg tatcggccac agtgatcgcc agaatgctgt ccgttgtggt ctcgacgcgt
     2461 atgccgaccc cgcctttttc ggtgaacttg acggcgtttc cagccagatt catgacgacc
     2521 tgccgcagac gagcgccatc gctgacgatc tttgcgggca cggcgcgatg aacaaatccg
     2581 gcgatctcga tgcccttgtc ctgggcgcgc ggcgacagga gttcgacgac gccttcgaca
     2641 agcgtttcga tggcgaccgt ctcggaggcg atttccagct ttccggcctc gatcttcgag
     2701 aaatccagaa tctcgttgat cagcgacagc agcgcctcgc ccgatgcatc gatggcgctg
     2761 gcgtagttct cctgctccgg actgagtccg gtgtcgcgca acaacccgac catgcccagg
     2821 acaccgttga gcggcgtgcg catttcgtgg ctgaccgtag ccaggaatcg ggacttcgcc
     2881 ccgaccgctg tctctgaatc accgccagcc gtcatgtcac ggccgaccgc cactacactg
     2941 cttccctcgc cccgtccgat ggtcgtgaag gtccaacgga tggtccggtc ggccaggacg
     3001 gtctcgaccg atccttcgcg gcgcgtgccc ttcagagtcc caaaggcctc gaagtaaacc
     3061 ttggcgccat cagcgtgcct tgcccacagg gcgttgaagg cagcgctccc ttcaaggatg
     3121 atgccgttcg aatccagctt gacgtaaaga tcgcccgatt tctcgatcag cgccttcaag
     3181 ccactgacgg tctcacgggt ctcgtcgagt ttggcctcat gctcctggag gcgcgtgcgc
     3241 agcacctgct tgcgaaggcg cagccttgca acgtcgcgaa tcatcgcgac gcaggccgcg
     3301 agcgccagaa ccgccgctcc gattgccgtg aaactcgcgg cgctccatgc gcccgcaagt
     3361 ggtgtcccat ccatttggcc gccccgatct ggctcttttg ccgacagcat gccgcccaag
     3421 ggttgcatcg aggtttgtgc accgccacca atccaaagcg tggttaaggc ggcgttcccg
     3481 ggcgcgccgg gttggtcctg acctcaggcc atcggcatga ctcggtagga cagcgcctct
     3541 gccagatgga ttcggctgac ccggatctcg ccatcgagat ccgcgatggt gcgcgcgact
     3601 ttcatgacac ggtggaagcc gcgagccgtc agccggaact tttcggatgc gtcgcgcagc
     3661 agcttggcac cggcatcgtc cggccgggcc acctcctcga tggcggcagc cgaagttgtc
     3721 gcgttggtgc ccgtcgccaa ccccatcgct gcgtagcgct cggcctgccg gagccgtgcc
     3781 gcagcaacac gcgccgcgac ctgcgctgac ccttccgagg gtggaggcag gaccaggtcg
     3841 gcggccgtga cggcggcaac ctcgatcatc agatcgaacc ggtcgatgag cggaccagag
     3901 agccgcgcct ggtattgcgc catgcatttc tcattgggcg cgcgcttgca ggcatatccg
     3961 ggctcggtcg cgcggccgca cttacaggga ttcatcgccg ccacgagctg gaaccgcgcc
     4021 ggataagtca cccggtgatt ggctcgcgac accgacactt cgcctgattc cagcggctga
     4081 cgcagggaat ccagcgcctg gggctggaat tccggcagtt cgtccaggaa cagcacgccg
     4141 tggtggctga gcgagacctc acccggtcgg gcgtgcagcc cgccgccggt cagggcggcg
     4201 atggaggccg aatggtgcgg cgagcggaac ggccgccggt ccgtcaacgc gccgccggta
     4261 agcaatccgg cgaccgaatg gatcatcgag acttcgagaa gttcgcgcgg gcccaacggc
     4321 ggaaggatcg acggcaggcg ctgcgccagc atcgacttgc ccgcgccagg cgggccggac
     4381 atcagcaggt tgtgacctcc agccgcagcg atctccaggg cgcgcttggc gttttcctgc
     4441 cccttcacat cccgaaggtc gggcagcacc ccttcgcccg accgcactgc ggggtgcggc
     4501 cgcgccatga cctgcgtgcc cttgaaatgg ttcgcgagct ggatgaggga cgcgggcgcc
     4561 agcacgtcca tgtcgccgcc ggcccaggcc gcctcgggac cgcaggattt cgggcagatc
     4621 agcccgtggc cgcgcgcatt ggccgccacg gcggcaggga gcacaccggc caccgccgag
     4681 atcgccccat ccagcgccag ctcgcccaga acggtgaagc cgtccagcgc atccgacggc
     4741 agggcgccga tcgccgccat gatccccagg gcgatgggca gatcgaaatg gctgccttcc
     4801 ttcggcagat ccgccggcgc cagattgacc accaccctct tggccggcat tgccagaccg
     4861 gaggcggtga gcgccgccct gacgcgctcc ttagactcgg ccaccgcctt gtccggcagt
     4921 ccgaccagaa cgaaagcgac attgccggag gccacctgga cctggacgtc gaccggccgc
     4981 gcctcgatgc cttcgaatgc gactgtcgcg acccgtgtga ccatgcgccc tgtccgttcc
     5041 tgcaagacta tcgaaagtct gtgacgaaca gcaagggcaa ttgctcgccc cgtctacgaa
     5101 cttccgatga gtattccgga aagcagcaca agaacgccgc cgacaatgac ctgcagcgtc
     5161 gcctgccaga acgggctttc catgtatttt gtgcggatcc acgaaatcgc cagcagttcg
     5221 atggcgacga tcaccgccgc catggccgta gcgatgtaga agccgttggg ccaggaatca
     5281 ggcacgaggt agggtatggt gtggccgaga ccgccgatca tggtcatcat cccgcacacc
     5341 agcccgcgca tccagggcga gccgcgtccg gtgatgatgc catcgtcgga cagggcctcg
     5401 gtcagcccca tgctgatgcc cgcgcccacc gacgccgcga gcccgaccag aaatgtctga
     5461 tggttcgagt gcgtggcgaa ggcggctgcg aacagcggcg ccagcgtcga taccgatcca
     5521 tcgatcaggc cggcgagacc cggctggaca tactgcagga cgaacagacg gtgcttggtc
     5581 gccgcttcct catcctgagc cgagtccgtc aggtgcgctt cggcaagctc cgccgcgcgt
     5641 ttctcatgac cgcgctcgac ctccgccagg tggaggtaca actcccgcac cgatgtatcg
     5701 agggtctgcg tggcggcctt gtcataaaag ttcccggcct gaagctccat gattgccgct
     5761 tcgcggcgca tggtgtcggg cgaaaggttc ttggtccacc agacggaacg gcgcttgagg
     5821 aagcccttca catcctcgcg acggatcggc ggcagatgct cgccgaaccg gttgcgatag
     5881 gccgcaaaca gggaagcgcg atggtcacgc tcttcctccg ccatgtcatc gaaaatcttt
     5941 gcggaagccg ggaagtcgga gcggcacttt tcagcgaaaa cctgatagat tctcgcgtct
     6001 tcctcttcgt tggcgatcgc cagcgccaga acctcgcgtt cggtcagttc acggagagct
     6061 ctcatcggcc accacattag aacggttata aattaactat gccccctgtg cgcgccaatc
     6121 gcaaccgttg tcaattccgc gcatcccgcc catcgtcgtt cgcgtgactg cttccctcct
     6181 tgcccttgct ctcaagatgg gtctggtcgc ggccatcgtg gtgggcgccg cgtccatcgc
     6241 cgaacggtcg cgaccggcgg tcgccgcgct ggtcgccacc ctgccggttt cgatcggtcc
     6301 gacctacgtg ttgctggcgc ttgaccacga cgccgccttc ctgaccgccg ccgcgctcaa
     6361 gagcgcggca agcgtcggcg cgaccatggc cttcatcatg gcctatgcca acgcgctcac
     6421 ccgtttcggg ccggccagtg cgcttgccgc ggcctattgc gcctggatac ccgcggcctg
     6481 gtttgtccat caccatgcct ggacggttgg tgaggtcgtc atgtttgtcg ccctgacgag
     6541 tattgcggga gcgatcgcca tgcgccgtct gcgccatcat caggcggaat cgtcgggcca
     6601 gcgacgccga tgggacgttc cggcacgggc tgccggagtt gtcgcgctcg tcggcgccat
     6661 caccgcgctg agcgaatcgc tgggccccag cggctcggga acgctcgcca acttcccggt
     6721 cgtgatgacc agtgtcacgc tgatcacaca tttgagactg ggcgggcttg ccgccgccgc
     6781 catgctcgcc aactcgaccg gcgggatggt cggggtcagc gccgcactgc tcactgtaca
     6841 tttcgccatt ccggtgctgg gcgccgcgct agccctgctg acgggattgg ttttctgcat
     6901 agtctggaac ctggggatat ttctgcgcgc acaccatcgt ttctaaagaa gaacgtttcg
     6961 ttcttttttt cagttgccat tcttcctgtt cgttttatag aacactccgc atgtcattcc
     7021 aaacacagga tggatcttag gccatgaagc tctcccgtcg cggtttcctt gcaacggccg
     7081 ccagcgtggc gctcgcccgt cccgccctgg cgcagaagct ctcgctgatc accctggact
     7141 gggcgaccta caatccgctc agcctgatca tccgcgacaa caagtggctg gaggaggatt
     7201 tcgcaaagga gtccgtggcc atccgctggg tgcagtccgc cggatcgaac aaggcgctgg
     7261 aattcctcaa tgcctcgtcc atcgacttcg gctcgacggc cggagcggct gccctgctcg
     7321 gccggatcgg cggcaatccg atcaggtcgg tcgcagtcta ctcgcggccg gaatggacag
     7381 cgctcgttac caagggcgac aaggtcaaga cagtcgccga cctcaagggc aagtccgtcg
     7441 cagtgacgcg gggaaccgat ccgcacatct tcctgatccg cgccctgctc gatcatggtg
     7501 tggcggcgag cgacgtcaaa ttcgtgctgc tccagcatgc cgatggcaag acggcgctgg
     7561 tccgtggcga tgtcgacgcg tgggccggcc tcgatccgat catggcacag gccgaggtcg
     7621 aggatggcgc gcgcctgttc taccgcaagc cggagaacaa cacctggggc atcctgaacg
     7681 cgcgcgagga tttcgccgcg aaaaacccgg agatcgtcaa acgggtgatc gcggtctacg
     7741 agaaggcgcg caagcatgcg ctggccaatc cgaaactggt ggtagaggcg ctggtcaagg
     7801 ccgccaggct gccggacacc gtcatcgcca agcagatcga acgcacggat ttcggaacga
     7861 gtgtcatcgg cccggtccag gccgaggcga tcgagcaggc cggccttgca ctgcaaaagg
     7921 ccgaggtcat caagggggac gtcaacgtca aggcaacggt cgcagccctg atcgatgcca
     7981 gtttcacctc gggtctgaag tcctgacgct cggggctggc tgaccatgac ggcaaacgac
     8041 ttggcgctcc agcaggcagg cgaagcggcg tccgggagtg aggggcagcc ggcagcagcc
     8101 ggctggctca agggccttgc cgtcggcgtc gccctgccgg cgctgctcgc gcttggctgg
     8161 gaggtcgccg ttcgggccgg cgtctccaac ggcaggctgg tgcccccgcc cagccggatt
     8221 ctggcgacgc tcgtcgatct gtggcgaacc ggggaccttg tcatgcatat cgtggcgacc
     8281 ctgcgccgcg tggcactcgg gttcgccttc ggcgcggtcg ccggcacgat cctgggcggt
     8341 atttccgggg cgtccgccat gttccgccgc ctgaccgatc cgatgttcca ggggttccgc
     8401 tcggttcctt cgatcgcctg ggtgccgttg ttcgtgctgt ggttcggcat tttcgaggcc
     8461 ccgaaagtca tgctgatcgc ggtcggggta ttctttccgg tctatctcgg ggtccttggc
     8521 gccatccagt cggtcgaccg gaaactggtc gaggtcgggc aggtgtttcg gctgtcgcaa
     8581 gcgcagatgc tgcgccgcat cctgctcccg gccatcctgc cgagctgggc ggtcgccctg
     8641 cgttccgggc tcggtctggg cttcatgttc gtcgtcgcgg ccgaactgat gggcgcgagc
     8701 gaggggcttg gctacctgct cgtcgatggc cagcagctcg gcaagcctga ccagattgtc
     8761 gccgcgatca tcgtttttgc gctgctcggc aagatcgccg atgccgtcct cgtgcgcctc
     8821 agcgccccgc tgctggcgtg gcaggacacg gcacgcccgg ccctgtgacg cgatgctgac
     8881 catcgaagcc ctgaccaaga cctacgccgg cggcacgacg gcgctgtcca acgtgtcgtt
     8941 gtcgatgcag gccggtgaaa tcgtggcgct ggtcggcggt tccggctgcg gcaagaccac
     9001 gctgcttcgc ctggtcgcag gactggatcg cccgagcggc gggtccgtcg ccgtcgacgg
     9061 caacgtcgtg acagagccga acgcggctgt cggcctgatc ttccaggaac cgcgccttct
     9121 gccctggctg tcagtcgccg acaacatcgc cttcgggctg aagcatctgc cacggaagga
     9181 gcagagggcg cgcgtcgatt tcgcgctcat gcgggtgggc ctcgccggct acaatgatcg
     9241 ctggccgcgc cagctctcgg gcgggcaggc gcagcgcgtc gccatctcgc gggcgctcgc
     9301 gccggagccg aaagtcctgt tgctggatga accgttctcg gcgctggatg ccttcacccg
     9361 cgccagcctg cacgatcacc tgctcgcact atgggctgcg cagcggccaa ccatgctgat
     9421 ggtgacgcat gatgtcgagg aggccgtcac gctggccgat cgcgtcgtgg tgatgcagcc
     9481 acggcccgga cggattttcg cgcagatcgc gatcgacttc ccccgcccgc gcgatccgct
     9541 gtccgacgct ttcatcgcgg ccaagcggcg gatactggaa gcgctggacg gttcgctgag
     9601 cgaatcgcgc cccaagccca agccggacga gtctccggcc gctgcctggt ggtgatttct
     9661 ctttaatatc aaggatttgt atcatggacg ccgaagccct ccgtacacta caggccccgc
     9721 tgaaggaccg ctacaagtcc gactccggcg cggcccggct gaccttgaag gccagcggcg
     9781 aaatcgacga gagcaagatt gcctgcaaga tcgagaccgg acgggcgatc gccgaggccg
     9841 ggctgcatcc cctgaccggg ggaacgggcg cggaactctg ctccggcgac atgctgctgg
     9901 aggcgcttgt cgcctgcgcc ggggtgactc tcaaggccgt ttcgacggcg atcggcgtca
     9961 atctccagcg cggccgcgtc accgcggaag gcgacctcga tttccgcggc acgctcggcg
    10021 tcgccaagga cgcaccggtt ggtttctcgg cgatccggct cacgttcgac ctgaaaacgg
    10081 acgcgacgga cgagcaggtt gcccagctca tcaagctgac ggagcgctac tgcgtggtgt
    10141 tccagaccct gcgcaccagc cccgccacgt cggtcgaaac gctcgtagaa cgctgactcc
    10201 tcggggccga acaggcggct tcgaggcacg gaacgcccgt gttccggcaa cgatggtttc
    10261 aagtctcggg cggccggatc gccttcagcc ggtagagcgc ctccagcgcc tcgcgcgggg
    10321 tcaggtcatc gaggtcaagg gcggccagcg c
//
LOCUS       DAOMXN010000073        10252 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_3478_length_10252_cov_11.046386, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000073 DAOMXN010000000
VERSION     DAOMXN010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 10252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10252
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3478_length_10252_cov_11.046386"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="PK812_06990"
     CDS             complement(<1..522)
                     /locus_tag="PK812_06990"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HRJ69338.1"
                     /translation="MRQAAKVDGRRKRSETSRNRIVDALMALVQEGHAAPAAEQVAER
                     AGIGLRSVFRHFSDMDSLYAEICGRIAAGLEASARQPFVAQDWRGQLFEMIDRRAFVY
                     EVIAPFQRAAEAHRHRSPALRESHVRLVATLRGLLVGRLPLEVAAQAPLVEALDALLS
                     FETWNRLREHQRLG"
     gene            complement(525..1637)
                     /locus_tag="PK812_06995"
     CDS             complement(525..1637)
                     /locus_tag="PK812_06995"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRJ69339.1"
                     /translation="MRARTKIALGTLALVLVAATAAWSFRGEIAVALVKRTVEQALTG
                     DPLAALPDGLHLGLCGAGSPFADARRAGPCVAVIAGRRLFVVDAGDGAARNLQLMNLP
                     PGQIEAVLLTHFHSDHIDGLGGVMVQRWVGGGHERPVPVHGPTGVEAIVAGFNQAYAA
                     DRGYRVAHHGSATVRPAGAGGEARPFQLGAPRAIVARAEFREVTVLESEGLKITAFEV
                     SHAPIEPAVGYAFTYKGRRLVISGDTTADARVETAAKGADLLVHEALSPRLVGIIGAA
                     AKTAGRENIAKIMADIVDYHATPEQAAALAQRAGARHLLLHHIVPALPSGLLEGPFLG
                     KARTLFSGTVQVGRDGDFISLPAGSDAILVSNRLRQ"
     gene            1730..2632
                     /locus_tag="PK812_07000"
     CDS             1730..2632
                     /locus_tag="PK812_07000"
                     /inference="COORDINATES: protein motif:HMM:NF002043.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HRJ69340.1"
                     /translation="MKVLRTPDERFDGLAGYSFAPHYLDVASHGGPALRLHYVDEGPR
                     EGAPVLLMHGEPSWSYLYRHMIPPIAAAGRRVLAPDLIGFGRSDKPADLEDYTYERHV
                     DWMSQWLTGLDLNHITLFCQDWGGLIGLRLVARFPERFAAVIVGNTGLPTGEGEVPLA
                     FKLWLAFSQNSPVFNVGWLVSKGTVRGLSASEKAAYDAPYPSNAYKAGARRFPALVPI
                     TSDHASVAENRKAWEVLEQFDKPFVTAFSDKDPITKGGYRIFQSRVPGAKGQPHARIR
                     DAGHFLQEDKPMELVELILRVAPT"
     gene            2629..3750
                     /locus_tag="PK812_07005"
     CDS             2629..3750
                     /locus_tag="PK812_07005"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRJ69341.1"
                     /translation="MSGPHIFWGWHLSYYAGKLRCYLAWKGIPFEDRAVDFVTLAFRI
                     KRRFGAPVMPVVVTPEGEWLQDSSAIIDLFEARFPDRPVVPEAPVQRFAACLLETWAD
                     EWWIPVAMHTRWSYPENYALFEKDAGDALLPMLPRFMKRMAVARVARLLRSYLPRVGI
                     TADQVPVLDAWTRESLEALDQHFAQFPYLLGGRPTLADFGLAGPMYGHLGRDPWPKRE
                     LVAQRPHLRAWIDRMASPPAGDAGALAADDAIPATLEPILKAIVSEFLPMIEAILGEV
                     DAFLQVKPGGARLPRSLGAVSFPMGSGRYSRAAMPFALWKMQRLLSLYRALPGEGQSR
                     VRRWLAGLGGERLLDLPIPRLQREGLLVSIQADARVDRS"
     gene            3747..5603
                     /locus_tag="PK812_07010"
     CDS             3747..5603
                     /locus_tag="PK812_07010"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HRJ69342.1"
                     /translation="MKRLGCTIASLCFAVPCIGGAGWRRIRDAANALVLAVGVVFAAL
                     PPAHAQTAKRPNIVFVLFDDAGFSDFGAYGSEIETPNIDAIAAAGVRMTNFHTPSTCE
                     SSRAMLHSGVDHHRAGMGTLQPVIAESQKGKPGYEGYLSDQVHSIGRLMRDGGYATYF
                     SGKWNLGTGIDRAPGRYGWDRYVALEQTGADNFEAKVYAPLSLESVWWEDGKRLSLPP
                     DFFSSRFYVDRLIRYIDEGRGAGKPFFGILSLQAVHSPLQAPQADIEKYARRYEAGWD
                     AIREERYQRQVGMGLFKPGLRMLASTGTSEWSKVPEEQRRDYARKMAVFAAMLDNADR
                     EIGRLKDHLRSIGELDNTAFIVMSDNGADAFDLSTINPLFRLWYRFHYALDRERMGGR
                     GSYVHYGADWARVSNTPLTSFKGSASEGGLRVPFIISYPPRVSPATSDAFAFASDFLP
                     TVLDLAGIAMPGEIYNGRKLHRPVGSSMMPHLEGKSAVIHPKERMFGFEGTGSKAVYK
                     DGYKLVWDFDPEIPSRWKLFRIDEDPTEANDLSEREPERLTAMLAEMSRYESENGVVA
                     PEPGYDPFRQLLRNNYGVLARQLGPVLAPILLILLAIVAAPIVVWRYRRRRS"
     gene            complement(5943..6017)
                     /locus_tag="PK812_07015"
     tRNA            complement(5943..6017)
                     /locus_tag="PK812_07015"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5983..5985),aa:Val,seq:gac)
     gene            complement(6098..6460)
                     /locus_tag="PK812_07020"
     CDS             complement(6098..6460)
                     /locus_tag="PK812_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006199474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRJ69343.1"
                     /translation="MTKILLAEDDNDMRKFLVRALQNAGYDVASFDNGKSAYHRLREE
                     PFELLLTDIVMPEMDGIELARRATELDPDIKVMFITGFAAVALNPDSQAPKDAKVLSK
                     PFHLRDLVNEVKKLLQAA"
     gene            6694..7566
                     /locus_tag="PK812_07025"
     CDS             6694..7566
                     /locus_tag="PK812_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HRJ69344.1"
                     /translation="MGEDIATPFEILEPRTVVSPFLFNSPHSGDCYPAEFLAMARLGL
                     RDMRCSADLFVDQLFLGVVERGIPLMRAHFPRSFLDVNREPYELDPRMFEGRMPAFAN
                     TRSLRVTSGFGTIARYAGEGQEIYTRRIPVDEALRRIDHYYLPYHAALRHRLNAIQRQ
                     FSQAYLIDCHSMPSGGGRTARNPDVVLGDRFGTSCTPALMDFVETFLVGAGLVVARNQ
                     PYAGGFITEHYGNPVAGLNALQIEINRSLYMDESLQSLHSGAGLITGVLMDLADSLIS
                     LTAFSTPSLRFAAE"
     gene            complement(8029..8979)
                     /locus_tag="PK812_07030"
     CDS             complement(8029..8979)
                     /locus_tag="PK812_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRJ69345.1"
                     /translation="MTKVLDKLVVIPANPMPEGGDIHGLAAADGAGLRSAYWPAADAR
                     GTVCVFPGRSEFIEKYFEVIGELRLRGFAVAALDWRGQGGSERELSDPRKGHIDDFDL
                     FLRDLDAFLDHLERVGAPKPWYGLAHSMGGAILVLATARGEKRLKRLVLSAPMLGLHG
                     AADATIARHIIQAANAAGLGAAYVPGAGARAPSAYQPFQDNPLTSDPDRYQRQCDIMI
                     AGPDLTIGPPTIAWLAAAFRLLNAMQAPDFGVTMGIPMLVVAAGADTIVSTSMVETLT
                     ARFRGAHCITIPGARHELLMERDPIRAQFWAAFDAFVPGS"
     gene            complement(9090..9470)
                     /locus_tag="PK812_07035"
     CDS             complement(9090..9470)
                     /locus_tag="PK812_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69346.1"
                     /translation="MHSPKGEGLSRVSIALTGAAVLAAVSAGLLLYLESHEAPPPPVP
                     SIAMAAEPPATVTPETIRRSMLMLGFRDVRNVRQRGQHFSADALTGTGLEARVVIHGT
                     TGAVLGIRVIEVATSTRLADTGRR"
     gene            9696..10145
                     /locus_tag="PK812_07040"
     CDS             9696..10145
                     /locus_tag="PK812_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017483557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HRJ69347.1"
                     /translation="MRTFDLAPLYRSTVGFDRLFSLFAEPGNGEPGGGYPRYNIEKTG
                     ENDYRITLAVAGFAEADLTVEVKEQMLVVRGEKAARADDAAIEVLFQGIAARAFERRF
                     QLAESVVVAGARLENGLLHIELKREVPEAKKPRLIPIGAGPASESRH"
BASE COUNT         1748 a         3279 c         3341 g         1884 t
ORIGIN      
        1 gccaagccgc tgatgttccc gcagccggtt ccacgtctca aagctcagca gcgcgtccag
       61 cgcttcgacc aggggcgcct gcgcggcgac ctccaaaggc agacgcccca cgagtagccc
      121 tcgcaacgtc gcgacgaggc gtacatggct ttcgcgcagc gccggcgaac ggtggcgatg
      181 ggcctccgcc gcgcgctgga acggcgcgat cacctcgtag acgaaagcgc gtcgatcaat
      241 catctcaaaa agctgcccgc gccagtcctg cgcgacgaaa ggctggcgcg cggatgcttc
      301 aagtccggct gcgatgcgtc cgcaaatctc ggcgtagagt gaatccatgt cgctgaaatg
      361 ccgaaatacc gaacgcagtc cgataccggc gcgctccgcc acctgctcgg ccgctggcgc
      421 ggcatggcct tcctgcacca gcgccatgag cgcatcgaca atcctgttgc ggctcgtttc
      481 ggaacgcttg cgccgcccgt cgactttcgc ggcctgtcgc atggctattg tctgagcctg
      541 ttcgagacga ggatggcatc cgatccagcc ggcagcgaga tgaagtcacc gtcccggcca
      601 acctgcaccg tcccggaaaa gagggtccgc gccttgccaa ggaacggtcc ctcgagcagt
      661 cccgagggca gggccggtac gatatggtgc aacagcagat ggcgcgcgcc ggcgcgttgg
      721 gcgagggctg cggcctgttc cggagttgcg tgatagtcca cgatgtccgc catgattttc
      781 gcgatgtttt cgcgtcccgc agtcttggcc gccgcaccga tgatgccgac aagtcgcggc
      841 gacagggctt catgcaccag caggtcggcg cctttcgccg ccgtttcgac gcgggcgtcc
      901 gctgtcgtgt cgccgctgat caccagccgg cggcccttat aggtgaaggc atagccaacc
      961 gcaggctcga tcggcgcatg actgacctcg aaggctgtga ttttcaggcc ttccgattca
     1021 agcactgtca cctcacggaa ttcggctctc gccactatgg cgcgcggcgc gccgagctgg
     1081 aacggccgcg cctcgccgcc agctccggcg gggcgaacgg tcgccgagcc gtgatgtgcg
     1141 acacggtagc cgcgatccgc cgcataggcc tggttgaatc cagcgacgat tgcctcgacg
     1201 cctgtcggcc catggacggg cacgggcctt tcgtggccgc caccaaccca gcgctgcacc
     1261 atgacgccgc caaggccgtc gatatggtcg gagtgaaaat gcgtcagcaa cacggcttcg
     1321 atctggccgg gcggcaggtt catcaattgc agattgcgcg cggccccgtc gccggcatcc
     1381 acgacgaaca gtcgccgccc cgcgatcacc gcaacgcaag gaccggcgcg acgggcgtcg
     1441 gcaaacggtg aaccggcgcc gcacaggcca aggtgcaagc catccggcag ggccgcgagt
     1501 ggatcgccgg tcaacgcctg ctcgaccgtg cgcttgacca gcgcgactgc gatctcgcca
     1561 cgaaaactcc aggccgccgt cgccgcgaca aggacaagag cgagcgttcc gagcgcgatc
     1621 ttcgttctgg cgcgcatggc ttgacggcct ccccgtgttg accgccattc gatgatatga
     1681 cactcattgt gtcaatatat cagatggggc gggcgcggga ggcattgcga tgaaggtgct
     1741 gcgaacgccc gacgagcgct tcgatggcct tgccggctat tctttcgcgc cgcactatct
     1801 cgatgttgcg tcccacggtg gaccggctct gcgcctgcac tacgtcgatg aaggtccgcg
     1861 cgagggggca cccgttctcc tgatgcacgg cgagccgagc tggagctatc tctaccggca
     1921 catgatcccg ccgattgccg cggcgggccg acgcgtgctc gcgcccgacc tgatcggctt
     1981 tggacgttcc gacaagccgg ccgatctcga ggattacacc tacgagcgcc acgtcgattg
     2041 gatgtcgcag tggctgaccg gtctcgatct gaaccacatc accctattct gccaggactg
     2101 gggcggtttg attggtctca ggctcgtcgc gcgtttccca gaacgcttcg ccgccgtcat
     2161 cgttggcaat accggactgc ccaccggaga gggcgaggtt ccgcttgcct tcaagctctg
     2221 gctcgccttc tcgcagaatt cgccggtctt caacgtcggc tggctggtgt cgaagggtac
     2281 ggttcgcggt ctcagcgcgt cggaaaaagc cgcctacgac gcgccttacc catccaatgc
     2341 ctacaaggcc ggggcgcggc ggtttccggc gcttgtaccg atcacatcgg atcatgcctc
     2401 ggtggcggaa aacagaaagg cgtgggaggt tctggagcaa ttcgacaagc ccttcgtcac
     2461 ggcgttcagc gacaaggatc cgatcacgaa aggcggctac cgcatcttcc agtcgcgcgt
     2521 gccgggcgcg aagggccagc cgcatgcgcg catccgtgac gccggccatt tcctgcagga
     2581 ggacaagccg atggaactgg tcgaactgat cctgcgggtt gcgccgacgt gagcgggcca
     2641 cacatcttct ggggctggca tctttcgtac tacgccggaa agctgcgctg ctatctcgcc
     2701 tggaaaggta tccccttcga ggaccgcgcc gtcgacttcg tgacgctggc gttccggatc
     2761 aagcggcgtt tcggcgcgcc ggtgatgcct gtggtggtga cgccggaggg cgaatggctg
     2821 caagattcca gtgccatcat cgacctgttt gaggcgcggt tccccgatcg gccggttgtg
     2881 cctgaagcac cggtgcagcg ctttgccgca tgcctgctgg aaacctgggc ggacgaatgg
     2941 tggatcccgg tcgccatgca cacgcggtgg agctatccgg agaactatgc gctcttcgag
     3001 aaggacgccg gcgacgccct gctgcccatg ctgccgcgct tcatgaagcg catggcggtg
     3061 gcgcgcgtcg cccggcttct ccggtcctat ctgccgcgcg tcggcatcac cgctgaccag
     3121 gttccggttc tggatgcctg gacgcgggag agtctcgaag cgctggatca gcactttgcg
     3181 caattcccct atctgcttgg cggccgaccg acgcttgcgg atttcggcct cgccggaccg
     3241 atgtacggcc acctcggacg cgacccatgg ccgaagcgtg aactggtcgc gcagcgcccg
     3301 catctgaggg cctggatcga ccgcatggcc tcgccgcccg ccggcgatgc aggcgccctg
     3361 gcggcggacg acgccatccc ggcgacgctg gagccgatcc tgaaagcgat tgtctcggaa
     3421 ttcctgccga tgatcgaggc gatccttggc gaagtcgacg ctttcctcca ggtgaagccg
     3481 ggcggggcgc gcctgccccg cagcctcggc gcagtttcgt ttccgatggg ctcaggtcgc
     3541 tatagccgcg ccgccatgcc gttcgcgctc tggaagatgc agcggctctt gtccctctat
     3601 cgcgccctgc caggcgaggg gcagtcaagg gtgcggcgct ggctcgcggg cctgggaggc
     3661 gagcgtctgc tcgacctgcc cattccgcgc cttcagcgcg agggactgct ggtatcgata
     3721 caagccgacg cgcgggtgga ccgatcatga aacgcttggg ctgtactatc gcgtcgcttt
     3781 gcttcgccgt cccctgcatc ggcggggctg gctggcgacg catccgtgat gctgcgaatg
     3841 cgcttgtcct ggccgtcggt gtggtctttg cggcattacc gccggcccat gcccagactg
     3901 cgaagcgtcc caatatcgtc tttgtcctct tcgacgatgc cggcttcagc gacttcggcg
     3961 cctatggcag tgagatcgag acgccgaaca tcgatgcgat cgccgccgcc ggcgttcgga
     4021 tgacgaattt ccacacgcct tcaacctgcg agtcttctcg cgcgatgctc cattccggag
     4081 tcgatcatca ccgcgccggc atggggacgc ttcaacccgt catcgccgaa agccagaaag
     4141 gcaagccggg atacgagggc tatctgtctg atcaggtgca ttccatcggc cgactgatgc
     4201 gcgacggcgg ctacgccacg tatttttccg gcaaatggaa tctggggaca gggatcgatc
     4261 gcgcgccggg ccgttacggc tgggaccgct atgtcgcgct cgaacagaca ggcgcggaca
     4321 attttgaagc gaaggtctac gcgccgctga gccttgagtc tgtctggtgg gaggatggca
     4381 agcgattgtc gctgcccccg gactttttct ccagccggtt ttatgttgac cggctcattc
     4441 gatacatcga tgagggcagg ggggcgggca aacctttctt tggcatcctt tcgctccagg
     4501 ccgtgcactc gcccttgcag gccccgcagg ccgacatcga gaaatacgcc aggcgctacg
     4561 aagcgggctg ggacgcgatc cgcgaggagc gttaccaacg gcaggtcggg atggggctgt
     4621 tcaagccggg cctcaggatg ctggcaagca cagggacatc ggagtggtcg aaagttccgg
     4681 aggagcagcg ccgtgactat gcgcggaaga tggcggtttt cgctgcgatg ctcgacaatg
     4741 ccgatcgcga aatcggtcgg ctgaaggacc atctccgaag catcggcgag ttggacaaca
     4801 ccgccttcat cgtcatgtcc gacaacggcg ccgacgcctt cgacctctcg accatcaatc
     4861 cgttgttccg gctgtggtat cgcttccact atgcgttgga ccgcgaacgc atgggcgggc
     4921 ggggcagcta tgtgcactac ggtgccgact gggcgcgtgt ttccaatacg ccgctgacct
     4981 cgttcaaggg atcggccagc gagggcggtc tacgcgtgcc gttcatcatc agctatcccc
     5041 cgcgtgtcag cccggcaacg agcgacgctt ttgcctttgc ctcggacttc ttgccgacgg
     5101 tgcttgatct cgccggaatc gcgatgccgg gcgaaatcta caacggccgg aagctgcata
     5161 ggcctgtcgg ctcgagcatg atgccgcatc tggagggcaa gagcgcggtg atccatccga
     5221 aggaaaggat gttcggattc gagggcacgg gaagcaaggc ggtctacaag gacggctaca
     5281 agctcgtctg ggacttcgac ccggagattc catcccgctg gaagcttttc cgcattgatg
     5341 aagacccgac ggaggccaac gacctttccg aaagggaacc ggagcgcctg acggcgatgc
     5401 tcgccgagat gtcgcgctat gagtcggaga acggcgtcgt tgcgccggaa ccaggctacg
     5461 atccgttccg ccagctcctg cgaaacaact acggcgtcct tgcccgccag ctcgggccgg
     5521 ttctcgcgcc gatccttctg atcctgctcg ccatcgtcgc ggcgcccatc gtggtctggc
     5581 gctaccgtcg tcgtcgatca tgacttgagc tacccggggc gcgcggggcg catcggcctg
     5641 tggggcgatg acgaaatcga cggccgactt catcgggctc tcggtcaggt ccgcctggcg
     5701 cgggctgaac cgcccggctt cctgcgacgc atgattctct cgggtgcctg gaagaatgtc
     5761 ggctcgtggt gttcacctcg cgccgcgaga gttccaagtg cggggcgcaa actggaaatc
     5821 ggcgcgagtg actggaacgc aggtgttttc ctgatgccat cgagaccatg accatcctgc
     5881 gccaaaaagc aaaaaccgaa gcggatcgct tcggcttttc gatagtcagc tgctgtctga
     5941 aatggtgggc gcgacaggga tcgaacctgt gacccctacg atgtcaacgt agtgctctcc
     6001 cgctgagcta cgcgcccttc catgtgccgg aaggcatgca ggaagccgct ctataggtgc
     6061 gagtgcggct caaggcaagt cgaatgtgcg gggtaggtca ggcggcctgc agcagcttct
     6121 tcacctcgtt gacgaggtcg cgcaggtgaa acggcttcga cagcaccttg gcatccttcg
     6181 gcgcctgcga atccgggttg agcgccaccg cggcaaagcc ggtgatgaac atgaccttga
     6241 tgtctggatc gagttccgtt gcccgccgcg ccagttcgat tccgtccatc tcgggcatga
     6301 cgatatccgt cagcaggagt tcgaacggct cttcgcgcag ccggtggtag gccgacttgc
     6361 cattgtcgaa ggaggcgaca tcgtaccctg cgttctgcag ggcgcgcacg aggaacttgc
     6421 gcatgtcgtt gtcgtcttcg gcgaggagta tcttggtcat ccgactcagg tccgcagcgg
     6481 gagatcgccg gagttaatct ccggggtcgt aaacaattcg tgaatccggc gcttgcccgg
     6541 attaacgaag gtggaaaacg gtcggcgaag cgccggttcg cggcgcgacc gccggttttg
     6601 gaacggcacg cttggtgtcc ccttgcattg gcggcgaagg tccggcagct ttccctgatc
     6661 tgttcgcgcc ggccgacact tggagaaaag atcttgggag aggatatcgc cacgccattc
     6721 gagatactcg aaccgcgaac ggtcgtctct cccttcctgt tcaattcgcc gcattccggc
     6781 gattgctatc cagccgagtt cctggcgatg gcgcggctcg gcctcaggga catgcggtgt
     6841 tcggccgacc tgttcgtgga ccagctgttc ctcggcgtcg tggagcgcgg cattccgctg
     6901 atgcgcgcgc atttcccgcg gtcgttcctc gatgtgaacc gggaacccta tgaactcgat
     6961 ccgcggatgt tcgagggccg catgcccgcc ttcgccaaca cccggtcgtt gagggtgacg
     7021 agcggcttcg gcacgatcgc ccgctatgcc ggggagggac aggagattta cacccggcgc
     7081 attccggtcg acgaggccct gcgccgcatc gatcactatt atctgcccta tcacgccgcg
     7141 ctgcggcacc ggttgaatgc gatccagcgt caattcagcc aggcctatct gatcgattgc
     7201 cattccatgc cgtccggcgg cggcaggacc gcccgcaatc cagatgtggt gctgggggac
     7261 cggttcggaa caagctgcac gccggcgctg atggatttcg tcgagacctt tctcgtcggc
     7321 gcgggtctgg tggtggcgcg caatcagcct tacgcgggcg ggttcatcac cgagcactat
     7381 ggcaatccgg tcgcggggct gaacgccctg cagatcgaga tcaaccgcag cctgtatatg
     7441 gacgagtcgc tgcagagctt gcattccggc gcgggcctga tcacgggcgt gctgatggat
     7501 ctggctgaca gcctgatcag cctcactgcc ttttcgaccc cgtccctacg gttcgccgcc
     7561 gagtagcggc aaaaaaagag ccgcctttcg gcggcccaag tctagggagg aaacgcccaa
     7621 ggagggcagc gggcgagggc gaaccatcga cccgcaccgc ctagttaata gtccataccg
     7681 gaacattcaa ggcatatttc ataggtatgc gcaggttccg gggtagtcat gcgaattacg
     7741 catttctagg cgggcgctgc ctggtgtcgg cgcggcgctg catgatcggc ttcgtctccc
     7801 tcatcggtca ttctccgcgc cagccacgac gtgcccggaa tccggtggtc ccggtgcgaa
     7861 aaagaaaaaa ggccgccttg cggcggccca agtctaggga ggaaacgccc aaggagggca
     7921 acaaaacgag ggcgaaccgt cgttttgcat atgcgaaagt aattgtgcgc tgcacaaatg
     7981 tcaagggtgc atctgcgaaa tggtgcaggc tcctggaacg aacatggttc aggaacctgg
     8041 aacgaacgcg tcgaaagccg cccagaattg cgcgcgaatc gggtcgcgct ccatgagcag
     8101 ttcgtgccgc gcgccgggaa tcgtgatgca gtgtgcgccg cgaaagcgtg ctgtgagcgt
     8161 ctcgaccatg ctcgtcgaga cgattgtgtc cgcgccggcg gccacgacca gcatcgggat
     8221 tcccatcgtc accccgaagt cgggcgcctg catcgcgttc agcaaccgga aggcggcggc
     8281 cagccaggcg atggtcggcg ggccgatcgt caggtcgggg ccggcgatca tgatgtcgca
     8341 ctggcgctga tagcgatcgg gatcggaggt cagcgggttg tcctggaatg gctggtaggc
     8401 gctcggcgcg cgggcgcctg cgcccggcac gtaggcggcg cccagtcctg ccgcattcgc
     8461 cgcctggatg atgtggcggg cgatggtcgc gtcggcggcg ccatgcagcc ccagcatggg
     8521 cgcgctcagg accagtcgct tcaggcgctt ctcgccgcgg gccgtcgcga gcaccaggat
     8581 cgcgccgccc atggaatggg caagtccata ccagggcttg ggtgcgccga cacgttcgag
     8641 atggtccaga aacgcgtcga gatcgcgcag gaacaggtcg aagtcgtcaa tatggccctt
     8701 gcgtggatcg gagagttcgc gctcggagcc accctggccg cgccagtcca gcgccgccac
     8761 ggcgaagccg cgaaggcgca attcgccgat gacctcgaaa tacttttcga tgaactcgct
     8821 gcggccggga aagacgcaga cggtgccgcg tgcgtccgcc gccggccagt aggcgctgcg
     8881 cagtcccgcg ccatcggcgg cggcgagacc atggatgtcg ccgccttccg gcatcgggtt
     8941 cgcagggatt acgacaagtt tgtcaagaac cttggtcatg ccttgcggtc gcagcgtccg
     9001 gacgggagcg ctttcgattg catcgcctta gggccgttgc aagccctttg tgctcgcgcg
     9061 ccggcgcgga tcgccgatcc gacccgccgt cagcggcgcc cggtatcggc aaggcgggtt
     9121 gatgtcgcca cctcgatcac gcgaatgccg agcaccgccc cggtcgtgcc gtggatcacg
     9181 acgcgcgcct ccagtccggt tccggtcagt gcgtcggcgc tgaagtgctg gccacgctgg
     9241 cgcacgttgc gcacgtcgcg gaagcccagc atcagcatgg atcgccggat ggtctcgggc
     9301 gtgaccgtgg ccggcggctc ggccgccatg gcgatggatg gcaccggagg cgggggcgct
     9361 tcatggctct ccagatacag cagaagcccg gcgctgaccg ctgcgaggac agccgcgccg
     9421 gtaagcgcga tggagacccg actgaggcct tctcccttgg gggaatgcat gcgatgcgtc
     9481 ctgaacacga tgtttgtctg cgtgccatgt tccccatccg ccctgaactg gcccggactt
     9541 gtgcgttcat ggcccgttca agccgttccg ggggcttgaa accacgccat cgcgctccca
     9601 cctcagcgtt gcgccggcca gtcggcggcg cacatcccgg tccggccgaa gcagcggacc
     9661 catcactccc tattgtcgct tccaggagga caatcatgcg tactttcgac ctcgctcccc
     9721 tctaccgttc gaccgtcggc ttcgaccgct tgttctcgct gtttgccgaa cccggcaacg
     9781 gcgagccggg cggcggctat ccgcgctaca acatcgagaa gaccggcgag aacgattacc
     9841 gtatcacgct cgccgtcgcc ggattcgccg aggctgacct caccgtggaa gtgaaggagc
     9901 agatgctggt cgtgcgtggc gagaaggcgg ccagggccga cgacgctgcg atcgaggttc
     9961 tgttccaggg catcgcggcc cgggccttcg agcgccggtt ccagctcgcc gaatccgtcg
    10021 tggtcgcggg tgcccgcctc gagaatggcc tgctgcacat cgagctgaag cgcgaggtgc
    10081 cggaagccaa gaagcccagg ctcattccca tcggcgccgg cccggccagc gagtcgcggc
    10141 actgacaccc ggatactccg gcagttcctg aaggggcgct tcggcgcccc tttttcgcgc
    10201 tccggcctat tccaggggcg cgggcggaac gtagggtgtc gccggcgcca gc
//
LOCUS       DAOMXN010000074        10154 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_3509_length_10154_cov_12.065749, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000074 DAOMXN010000000
VERSION     DAOMXN010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 10154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10154
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3509_length_10154_cov_12.065749"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..581)
                     /locus_tag="PK812_07045"
     CDS             complement(87..581)
                     /locus_tag="PK812_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69348.1"
                     /translation="MPLPRRAILALPLILLGATQAAAGPLLDRIFPRGQQVCYGRKAE
                     LAVPTSFRLIRITRPKRFQQYDGADSRHVRIVINFAGRDRRYEDNASCVEDGDRLRCL
                     SATCEGAGFLIEPGEAAGIRLRQGTVKTFVIWGCGDDKIKSIALRPEDQLVPMRRATG
                     ACIN"
     gene            754..2094
                     /gene="glmM"
                     /locus_tag="PK812_07050"
     CDS             754..2094
                     /gene="glmM"
                     /locus_tag="PK812_07050"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020094009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HRJ69349.1"
                     /translation="MTRKYFGTDGIRGRSNGLITAELAMKVGQAAGLIFRRGDYRHRV
                     VIGKDTRLSGYMIEYAMTGGFVSVGMDVLLLGPVPTPAVAMLTRSMRADLGVMISASH
                     NAYEDNGIKLFGPDGYKLNDDIEREIEALIDQDLGRRLSSSSSIGRAKRIEGVHDRYI
                     EFAKRTLPRNISLEGLRVVVDCANGAAYKVAPEALWELGAEVVTLGVEPDGFNINRDV
                     GSTAPQALQRKVRELRADIGIALDGDADRVMIIDEKGQAIDGDQLMAVVAKSFMDEGR
                     LSKPGVVATIMSNLGLERHLGDMGLTLARTAVGDRYVLEHMREQGYNVGGEQSGHIIL
                     SDYSTTGDGLVAALQVLAVVQREGKPVSEVCHRFEPLPQVLKNVRYRSGQPLQADSVK
                     TAIEQAKQRLSGCGRLVIRPSGTEPVIRVMGEGDDRDLVNAVVDEVVDVITRAA"
     gene            2364..3239
                     /locus_tag="PK812_07055"
     CDS             2364..3239
                     /locus_tag="PK812_07055"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRJ69350.1"
                     /translation="MGSLKTLAYAGAVAMVASSALFAAPASAADLGAPPIMAPPPPPP
                     MVDGMSGLYLRGDIGVGVMSSASMKSTLGPTIVAPNYMVDKYSFDNVAFAGVGVGYQF
                     NSFLRFDVTGEYRTTSKFSAFDSFNGTSFGMPCIKCYNLYSSSIDSSNLMANAYIDLG
                     TWSGLTPYVGAGIGFAHHKVSGLQDVGVAATIGGFGYAADTSQTNFAWALMAGVGMQV
                     TRNLTMDLGYRYMNMGSVDTNAIHCVLGNGLPNPNWCSRHTYDVNSHDVKIGFRYMFG
                     GDVAPAPAAPLIRKF"
     gene            3426..4595
                     /locus_tag="PK812_07060"
     CDS             3426..4595
                     /locus_tag="PK812_07060"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018235518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HRJ69351.1"
                     /translation="MTKPAAKPGILPRVANFSSGPCAKRPGWSLKNLADAPLGRSHRA
                     KIGKAKLKLAIDLTREILEIPADYKIGIVPASDTGAVEMALWSLLGARGVDMLAWESF
                     GEGWVTDVVKQLKLADVREIKAPYGELPNLKKVDTKNRDVVFTWNGTTSGVRVPDADW
                     IAADREGLTICDATSAAFAQPLDWPKLDVVTYSWQKVLGGEAAHGMLILSPRAVKRLE
                     TYKPAWPLPKIFRLTSGGKLINGIFEGETINTPSMLCVEDYIDALTWAKKVGGLKGLI
                     KRADANTRVLSKWVKATDWIDFLAVKPKTRSNTSVCLKFTDPKVTALDAGKQAEFAKN
                     VVALIEKEKAGYDLGHYRDAPPGLRIWCGATVQARDVKALIPWIEWAFATERAKL"
     gene            4780..6369
                     /gene="serA"
                     /locus_tag="PK812_07065"
     CDS             4780..6369
                     /gene="serA"
                     /locus_tag="PK812_07065"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018042763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HRJ69352.1"
                     /translation="MTKPRVLVSDALSKAAIQIFTDRGVEVDFQPDLGKDKDKLAAII
                     GNYDGLAIRSASKVTAKLLETADRLKVIGRAGIGVDNVDIPAATARGIIVMNTPFGNS
                     ITTAEHAIAMMFALARQIPAADLSTQAGKWEKNRFMGVELEHKTLGVIGCGNIGSIAA
                     RKAIGVGMKVIAFDPFLSEDRARDLGVEKVELDALLARSDFITLHTPLTEKTKNILSR
                     ENLAKTKKGLRLINCARGGLVDEQALADAITAGHVAGAAFDVFSEEPATTNPLFGLPN
                     VICTPHLGASTNEAQENVALQVAEQMSDYLLKGGVSNAVNFPSISAEEAPKLKPFVAL
                     AEKLGSFLGQLTEAPVKSIRIQYEGNVADLNVRPMTAAAVAGVLRAFMPEINMVNAPL
                     AAKEKGIAVEEVKRAADSEYDSRIFIEVVAEDMPRHAAGTVYHDGKPRIVEIRRIAMD
                     AEFAPHMLYIRNDDKPGFIGQFGSLLGEAGVNIATFNLGREKAGGDAICFVAVDEKVS
                     DDLLEKIEAIPQVKRARRLAF"
     gene            6447..6677
                     /locus_tag="PK812_07070"
     CDS             6447..6677
                     /locus_tag="PK812_07070"
                     /inference="COORDINATES: protein motif:HMM:NF020542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1902 domain-containing protein"
                     /protein_id="HRJ69353.1"
                     /translation="MRRFAIVRAAWDSEVSVWYVEESDIPGLATESPTLEGLKQRVRD
                     IVPDLLEGQDDVPDELEIEFIAHSHERVKTAA"
     gene            6788..6982
                     /locus_tag="PK812_07075"
     CDS             6788..6982
                     /locus_tag="PK812_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HRJ69354.1"
                     /translation="MTSYTPQIIDILRRNGFEYWRSGKDDHQIWRHKVTGKRVTVDSK
                     VLSRHNANKTLRDAGLPKAF"
     gene            complement(6991..7233)
                     /locus_tag="PK812_07080"
     CDS             complement(6991..7233)
                     /locus_tag="PK812_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69355.1"
                     /translation="MRLMIAVVAASAILAAPARALTCEALVLEEAIVFSKGQPKEKWF
                     KRSAATPEENARAWLASPKGQEQIAKDRARKRKGCV"
     gene            complement(7293..8204)
                     /locus_tag="PK812_07085"
     CDS             complement(7293..8204)
                     /locus_tag="PK812_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRJ69356.1"
                     /translation="MNSTPGHSQTARPLLLLTLTGICWAGNTIAGRMAVGEASPMAVT
                     ALRWIVSFALMSLFVRAEVRAIPALLRRRPLYLLAMGILGFTGFNALYYIAAHSTTAI
                     NLGIMQASTPLFVLAGAAAFHGVRASPALIAGLLAGIAGVVTVVSGGRLETLAALAFK
                     QGDLLILATSLMYAVYSLGIRDRPQDVSPITFFAAMAFIAALTSLPLVGLEMALGAFQ
                     APTLKGWAVIIWVAVFPSLLAQLWYISVVATIGANRAGFIFNLIPIFASILAVILLGE
                     DFGWHHAAGLALVLGGIAFAERFGKGK"
     gene            8287..9153
                     /locus_tag="PK812_07090"
     CDS             8287..9153
                     /locus_tag="PK812_07090"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HRJ69357.1"
                     /translation="MTLRFLVIEGNNAEGRERQRTRLGRMASEGYSGCLRYLAPGSVT
                     DICMPADEGANLPSERELREAYDGVVITGSALHVWEGHPDALRQVAFARTVFRAGVPF
                     FGSCWGLQVASAAAGGTVEKNPLGREAGIARAITLNEKGLRHPMLAGRPGVFDAPCVH
                     LDAVTEPAPGTTILASNAMAPVQAAEIHHEGGVFWGVQYHPEFTLTHLANILSLSLPM
                     HVEEGRFADEAQGEAWCNDMRSICRDPSLSHVAWRYGLGPELLDRQKRLTEIGNFIEH
                     WVKPHAIKRAAA"
     gene            9274..>10154
                     /locus_tag="PK812_07095"
     CDS             9274..>10154
                     /locus_tag="PK812_07095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HRJ69358.1"
                     /translation="MSHPIATALLRPFLFTGCAIAALSAAAPLGAAEMEAKSRIAAVT
                     VYPDGAIVNRSVDVDLPAGSTLLVFRGLPATIDPASLRVEGQASGALTLGSVESRMTP
                     ADPGKATGPSDEQLRTLRLDRDRLATDLESLESRRAMIQRYAQAGPDRQGEQGRSLEV
                     EKWAQAWEAVGAALTRVNEDVRRARLAIRDVEDKIRAIEAADSARRQRTTPEREFTLA
                     LEAGQALKGTLTLSYRVGGASWRPAYDATLETGEGGKAAGLKLTRRALVTQRTGEDWS
                     DVAMTLSTQLRSGAYRLR"
BASE COUNT         1818 a         3290 c         3400 g         1646 t
ORIGIN      
        1 ggctcggccg tgccctcgac aggccgcggc aagcccaagc ccgagggcgg ggccgaaccc
       61 ctgccgcagg cgtaggcgaa gccccgtcag ttgatgcagg cgccggtggc gcgccgcatc
      121 ggcacgagct gatcttccgg acgcagcgcg atcgatttga tcttgtcgtc gccgcaaccc
      181 cagatgacga aggtcttgac cgtgccctga cgcaggcgga tgccggcggc ttcgccgggc
      241 tcgatcagga aaccggcgcc ctcgcaggtc gcggacaggc agcgcagccg gtcaccgtcc
      301 tcgacgcagg aggcgttgtc ctcataacga cggtcgcggc cggcgaaatt gatgacgatg
      361 cgcacgtgac ggctgtcggc gccatcgtat tgctggaacc gcttgggccg cgttatgcgg
      421 atcagccgga agctggtcgg cacggcgagt tccgccttgc ggccataaca gacctgctgt
      481 ccgcgcggaa agatgcggtc cagcaacggt ccggcggcgg cctgtgtcgc gccgaggagg
      541 atcaggggca gggcaagaat ggcgcggcgg ggtaacggca tggcgctgac ctggacagga
      601 ttcggacgcc agaggatggg ccttgaacgg tcgtgttgtc cagtctgttc gccgcgacag
      661 ccgaaacgga cgggacaact ggcaatccgg tgggcggggc gctacagaag taccggaatc
      721 ggctacgctg cggcacatcg cggagaacgg cgcatgacac gcaaatactt cggcacggac
      781 ggcattcgcg gccgctcgaa cgggctcatc acagcggaac tcgcgatgaa ggtcgggcag
      841 gcggccgggc tgatcttccg ccgcggcgat taccggcacc gggtggtgat cggcaaggat
      901 acgcgccttt ccggctacat gatcgaatac gccatgaccg ggggcttcgt ctcggtcggc
      961 atggatgtgc tgctgctcgg ccccgtgccg acgccggcgg tcgccatgct gacgcgctcc
     1021 atgcgcgctg acctcggcgt gatgatctcg gcctcgcaca acgcctacga ggacaacggc
     1081 atcaagctgt tcgggccgga cggctacaag ctcaacgacg acatcgaacg cgaaattgag
     1141 gcgctgatcg atcaggatct cgggcggcgc ctgtccagtt cctcgtccat cggtcgggcc
     1201 aagcgcatcg agggcgtgca tgaccgctac atcgagttcg ccaaacgcac gctgccgcgc
     1261 aacatctcgc tggaaggcct gcgggtggtc gtcgattgcg ccaacggcgc cgcctacaag
     1321 gttgcgcccg aagcgctgtg ggagcttggc gcggaggtgg tgacgctggg ggtcgagccc
     1381 gatggcttca acatcaaccg cgatgtcggc tccaccgcgc cgcaggcgtt gcagcgcaag
     1441 gtgcgcgagc tgcgggccga catcggcatc gcgctggatg gcgacgccga ccgcgtgatg
     1501 atcatcgacg agaagggcca ggcgatcgac ggtgaccagt tgatggccgt ggtcgccaag
     1561 agcttcatgg atgaaggccg gctgtcgaag ccgggcgtgg tcgcgacgat catgtccaac
     1621 ctcgggctgg agcgccatct cggcgacatg gggctgacac tcgcccgcac ggccgtcggt
     1681 gaccgctacg tgctcgagca catgcgcgag cagggctaca acgtcggcgg cgagcagtcg
     1741 ggccacatca tcctgtcgga ttattccacg accggcgacg gccttgtcgc cgcgctccag
     1801 gtgctggcgg tggtgcagcg cgagggcaag ccggtgagcg aggtgtgcca tcgcttcgag
     1861 ccgctgccgc aggtgctgaa gaatgtgcgg taccggtccg gccagccgct gcaggcggac
     1921 tcggtcaaga ccgcgatcga acaggccaag caacgcctct cgggctgcgg gcgcctggtc
     1981 atccgtccct ccggcaccga accggtgatc cgggtcatgg gcgagggcga tgaccgcgac
     2041 ctggtcaatg cggtcgtcga cgaagtcgtc gacgtcatca cccgggccgc ctgattccgt
     2101 ccgtcgggct ggccgggacg cgcctgtcgg caggcggcgg cgattcggtg cgcgaggcca
     2161 gggggaggcg accgcggaat gcccggaaaa tgcttgccag cggctgcctg ctgtcaatgc
     2221 aagccattga aaacattcat tgtaaccaag gataaaggtt aattcttaac gttaagaggg
     2281 ccttaaggtt tcagtgcgaa attccctcct gttcgcaatg ccggtccgcc ctgatgaccg
     2341 gaccgagaca caagggattt cacatgggca gcctcaaaac cctcgcatac gcgggcgccg
     2401 tggcgatggt cgcatcgtcc gctctgtttg ccgcgcctgc ttccgccgcc gatcttggcg
     2461 cgccgccgat catggcgcct ccgccgcctc cgccgatggt ggacggcatg agcggtctgt
     2521 acctgcgggg cgacattggt gtgggcgtga tgtcgtccgc aagcatgaag tctaccctcg
     2581 ggccgacgat tgtcgctccg aactacatgg tcgacaaata cagcttcgac aacgtggcct
     2641 tcgccggggt gggcgtcggc taccagttca acagcttcct gcgcttcgac gtgacgggcg
     2701 agtatcgcac gacgagcaag ttctctgcct tcgacagctt caacggcact tcgttcggca
     2761 tgccttgcat caagtgctac aacctctact cgtcctcgat cgacagctcc aacttgatgg
     2821 ccaatgccta tatcgacctt ggcacctgga gcgggctgac gccatatgtc ggcgcgggca
     2881 tcggttttgc ccaccacaag gtttccgggc tgcaggacgt gggcgtggcc gccaccatcg
     2941 gcggatttgg ctatgccgca gacaccagcc agaccaactt cgcctgggcg ctgatggccg
     3001 gcgtgggcat gcaggtgacg cgcaacctga ccatggatct gggctatcgc tacatgaaca
     3061 tgggttcggt cgacacgaac gcgatccatt gcgttctcgg caacggtttg cccaacccga
     3121 actggtgttc gagacacacg tacgacgtca actcgcatga cgtgaagatc ggcttccgct
     3181 acatgttcgg cggtgacgtc gccccggcgc ctgccgctcc gctgatccgc aagttctgat
     3241 ccggcgctcg gacagcacgc tttcctgaac gcggccctcg ggccgcgttt tcgttttcgg
     3301 gcctgcggca aagtggcccc cttttccgga aatgtcttgc atcggggcag gcaggcgcgt
     3361 aatggcggcg gcgggacacg ccgccccaac gcggcgcgcc catctgcaag gatggagaca
     3421 ttgcgatgac caagcccgcc gcgaagccgg ggattctccc gcgcgttgcc aacttctcgt
     3481 ccggcccctg cgccaagcgc cccggatggt ccctcaagaa cctcgctgac gcaccgctcg
     3541 gccgctcgca tcgcgcgaag atcggcaagg cgaagctcaa gctcgccatc gacctgacgc
     3601 gcgagatcct ggaaattccg gccgattaca agatcggcat cgttccggcc tccgacaccg
     3661 gcgcggtcga gatggcgctg tggtcgctgc tcggcgcgcg cggcgtcgac atgctcgcct
     3721 gggaatcctt cggcgaaggc tgggtgacgg acgtcgtgaa gcagctcaag ctcgccgacg
     3781 tgcgcgaaat caaggcgccc tacggtgaac tgccgaactt gaagaaggtc gacacgaaaa
     3841 atcgcgacgt cgtcttcacc tggaacggca cgacgtccgg tgtgcgcgtg ccggacgcgg
     3901 actggatcgc cgccgatcgc gagggcctga ccatctgcga cgcgacctcg gccgctttcg
     3961 cgcagcctct ggactggccg aagctcgatg tcgtcaccta ctcctggcag aaggtgctcg
     4021 gcggcgaggc ggcgcatggc atgctgatcc tcagcccacg cgcggtgaag cggctggaga
     4081 cctacaagcc ggcctggccg ctgccgaaga tcttccgcct gacgtccggc ggcaagctga
     4141 tcaacggcat cttcgagggc gagacgatca acaccccgtc catgctctgc gtcgaggatt
     4201 acatcgacgc gctgacctgg gcgaagaagg tcggcggcct caagggcctg atcaagcgcg
     4261 ccgacgccaa tacgcgcgtc ctctcgaaat gggtgaaggc gacggactgg atcgatttcc
     4321 tcgcggtgaa gccgaagacg cgctccaaca cctccgtgtg cctcaagttc accgatccca
     4381 aggtgacggc gctcgacgcc ggcaagcagg cggaattcgc caagaacgtc gtcgcgctga
     4441 tcgagaagga aaaggccggc tacgacctcg gccactaccg cgacgcgcct ccgggcctgc
     4501 gcatctggtg cggcgcgacc gtgcaggcgc gcgacgtgaa ggccctgatc ccctggatcg
     4561 aatgggcgtt cgcgaccgag cgcgccaagc tctagccgac atcagcgatg gtccttctcc
     4621 ccgcgaggga gaaggtggcg cgagtgttag cggagcgccg gatgaggggc tgtcgctcct
     4681 cgcatttccc ttcacccggc ccttcgggcc accttctccc gcaaaagggg agaggggtct
     4741 tgcccggctc gcgccgcggc cgacttcaag gagcttgcta tgaccaaacc ccgcgttctc
     4801 gtttccgacg ccctgtccaa ggctgcgatc cagatcttca ccgatcgcgg cgtcgaggtg
     4861 gatttccagc cggacctcgg caaggacaag gacaagctcg ccgccatcat cggcaattat
     4921 gacggcctcg ccatccgctc cgcctccaag gtgacggcga agctcttgga aacggcggac
     4981 cggctgaagg tgatcggccg cgccggcatc ggcgtcgaca acgtcgacat cccggccgcg
     5041 accgccaggg gcatcatcgt gatgaacacg cccttcggca attccatcac cacggccgaa
     5101 cacgccatcg ccatgatgtt cgcgctggcg cgccagatcc ccgcggccga cctctccacc
     5161 caggccggca agtgggagaa gaaccgcttc atgggcgtgg agctggagca caagacgctc
     5221 ggcgtcatcg gctgcggcaa catcggctcg atcgcggcgc gcaaggcgat cggcgtcggc
     5281 atgaaggtca tcgccttcga tccgttcctc tccgaggatc gcgcccgtga cctcggcgtc
     5341 gagaaggtcg aactggatgc gctgctcgcc cgctccgatt tcatcaccct gcatacgccg
     5401 ctgaccgaga agacgaagaa tattctctcg cgcgagaacc tggcgaaaac gaaaaagggc
     5461 ctgcgcctca tcaactgcgc gcgcggcggc cttgtcgacg agcaggctct ggccgacgcc
     5521 atcaccgccg gtcatgtcgc gggcgcggcc ttcgacgtgt tctcggaaga accggcgaca
     5581 acgaatccgc tgttcggcct gccgaacgtg atctgcacgc cgcatctcgg tgcctcgacc
     5641 aacgaggcgc aggagaatgt cgcgcttcag gtcgccgagc agatgagtga ctacctgctc
     5701 aagggcggcg tttccaacgc ggtgaatttc ccgtccatct cggcggagga agccccgaag
     5761 ctgaagccgt tcgtggcgct ggccgaaaag ctcggctcgt tcctcggcca gctcaccgag
     5821 gcgccggtga agtcgatccg catccagtac gagggaaatg tcgccgatct caacgtgcgg
     5881 ccgatgactg cggctgcggt ggcgggcgtg ttgcgtgcct tcatgcccga gatcaacatg
     5941 gtcaacgcgc cgctcgcggc gaaggaaaag ggcatcgccg ttgaggaggt caagcgcgcc
     6001 gccgacagcg agtacgacag ccgcatcttc atcgaggtcg tggccgagga catgccgcgc
     6061 catgccgccg gcacggtcta ccatgacggc aagccgcgca tcgtggagat ccgcaggatc
     6121 gccatggatg ccgaattcgc cccgcacatg ctctacatcc gcaacgacga caagccgggc
     6181 ttcatcggcc agttcggttc gctgctgggc gaggccggcg tcaacatcgc caccttcaat
     6241 ctcggtcgcg aaaaagcggg cggcgatgcc atctgcttcg tcgcggtgga tgagaaggtc
     6301 tcggacgatc tgctcgagaa gatcgaggcc atcccgcagg tcaagcgcgc acggcggctg
     6361 gcgttctgat aacgctgtgg cgtcataaca attgcaggcg gccggatcat gcgcgatgat
     6421 ccggccgtta aattttggag gggttcatgc gccgctttgc gattgtccgc gccgcgtggg
     6481 acagcgaggt cagcgtctgg tatgtcgagg aaagcgacat tcccggtctt gcgactgaat
     6541 ctccgacgct cgaaggcctg aagcagcgtg tgcgcgacat cgttccggac ttgctcgaag
     6601 ggcaggatga cgtaccggac gaattggaaa tcgaattcat tgcccacagc cacgagcggg
     6661 tgaagacggc cgcgtgatgg gctgtgaata gtgggaacat tggggataac ggggacagag
     6721 acgcaagccg cagtaattca tgtgagtccg gggtaatttg agccgaatca cttaatcgag
     6781 gctctcgatg acctcctaca cgccgcaaat catcgatatc ctgcgccgca acggctttga
     6841 gtactggcgc agcggaaaag acgatcacca gatctggcgt cacaaggtca ccggcaaacg
     6901 cgtcaccgtc gattccaagg tgctgtcgcg ccacaacgcc aacaagacct tgcgcgacgc
     6961 cggccttccc aaggccttct gatccttcct ctacacgcag cccttgcgct tgcgcgccct
     7021 gtctttggcg atctgctcct gccctttcgg cgaggcgagc caggcgcggg cgttctcttc
     7081 cggcgtcgcc gcggagcgtt tgaaccattt ctccttcggc tggcccttgc tgaagacgat
     7141 cgcctcctca agcaccagcg cttcgcaggt cagcgcgcgc gcgggggctg cgaggatggc
     7201 gctcgcggcg acgacggcga tcatcaggcg catggcgttt tcccgttggg ttccgcgagg
     7261 tttccacgcg aaccccgcgc ggtcaatcgc ctctacttgc ctttgccgaa ccgctcggcg
     7321 aaggcgatgc cgcccagcac cagggcgagg ccggcggcgt gatgccagcc gaagtcctcg
     7381 ccgagcagga tcacggccag gatcgaggcg aagatcggaa tcaggttgaa gatgaagccg
     7441 gcgcggttcg cgccgatggt ggcgacgacg gagatgtacc agagctgagc cagcagtgag
     7501 gggaaaaccg ccacccagat gatcaccgcc cagcccttga gcgtcggcgc ctggaaggct
     7561 ccgagcgcca tttccagccc caccagcggc agcgacgtca gtgcggcgat gaaggccatg
     7621 gccgcgaaga aggtgatcgg cgagacatcc tgcggccggt cgcggatgcc gagcgaatag
     7681 accgcgtaca tcaggctggt ggcgagaatc agaaggtcgc cctgcttgaa ggcgagggcc
     7741 gccagcgttt ccagccggcc gccggagacg accgtgacca cccccgcgat gccggcgaga
     7801 aggccggcga tcagcgccgg ggaagcgcgg acgccgtgga aagccgccgc gccggccagc
     7861 acgaacagcg gggtcgaggc ctgcatgatg ccgaggttga tcgccgtcgt ggaatgggcg
     7921 gcgatgtaat agagcgcgtt gaatccggtg aagcccagga tgcccatggc gagcagatag
     7981 agcggccggc ggcgcaacag cgccgggatg gcacgcacct cggcacgcac gaaaaggctc
     8041 atcagcgcga aggaaacgat ccagcgcaag gcggtcaccg ccatgggcga ggcctccccc
     8101 acggccatgc ggccggcgat ggtgttgccg gcccagcaga tgccggtgag cgtcagcagc
     8161 agcagggggc gcgcggtctg cgaatggccg ggcgtggaat tcatgttgaa aaggccattc
     8221 actctttgtc taacaggtgt gcggcatagt gcatgttcca tccacgctta gaccaagacc
     8281 agcgccatga cactccgatt tctcgtgatc gagggcaaca acgccgaggg gcgcgaacgc
     8341 cagcgcaccc gcctcggccg catggcgagc gagggctatt ccggctgcct gcgctacctt
     8401 gcgccgggtt cggtcaccga tatctgcatg ccggcggatg agggggccaa cctgccgtcc
     8461 gaacgggaat tgcgcgaggc ctacgacggg gtggtgatca ccggctcggc gttgcatgtc
     8521 tgggaaggcc atcccgatgc cctgcggcag gtggcgttcg cgcgcaccgt ctttcgcgcc
     8581 ggcgtgccat tcttcggttc ctgctggggc ctgcaggtgg ccagcgccgc ggcgggcggc
     8641 acggtggaga agaacccgct cggccgcgag gcgggcatag cgcgggccat caccctcaac
     8701 gagaagggct tgcgtcatcc gatgctggcc ggaaggccgg gcgtgttcga tgcgccctgc
     8761 gtccatctcg atgcggtgac ggagcccgcg cccggcacga cgatcctggc ttccaacgcc
     8821 atggccccgg tgcaggcggc ggaaattcac cacgagggcg gggtgttctg gggggtgcag
     8881 taccacccgg aattcacgct gacgcatctg gcgaacatcc tctcgctcag cctgccgatg
     8941 catgttgagg agggccgctt cgccgatgag gcgcagggcg aggcctggtg caacgacatg
     9001 cgctcgatct gccgtgatcc ctcgctcagc catgtcgcct ggcgctacgg cctcgggccg
     9061 gaactgctgg atcggcagaa acggctcacc gagatcggca atttcatcga gcactgggtg
     9121 aagccccacg cgatcaaacg ggccgcggcc tagcaacccg gcccggggct gccgccgcga
     9181 ttgcgtcgcg gatcggccac aggaattccg caaggcgcga atgagttcgc gccagagcct
     9241 gacggcttgc gccgtccatt tccggaggta cccatgtccc acccgatcgc gaccgccttg
     9301 cttcgtccgt tcctgttcac cggctgcgcc atcgccgcgc tgtccgctgc cgcgccgctc
     9361 ggcgccgccg agatggaggc gaaatcccgc atcgcggcgg tcacggtcta ccccgacggc
     9421 gccatcgtca accgttcggt cgacgtcgac ctgccggcag gttcgacgct cctggtgttt
     9481 cgcgggctgc cggcgaccat cgatccggcc tcgctgcgcg tcgagggcca ggccagcggc
     9541 gcgctgacgt tgggcagcgt cgaatcccgc atgacgccgg ccgatccggg caaggcgacg
     9601 gggcccagtg atgaacagct gcgcacgctg cgcctcgacc gcgaccggct cgccaccgat
     9661 ctggaatccc tcgaaagccg ccgcgccatg atccagcgct atgcgcaggc cggcccggac
     9721 cggcagggcg agcagggcag gagccttgag gtcgagaaat gggcgcaggc ctgggaagcg
     9781 gtcggcgcgg cactgacccg ggtcaacgag gacgtgcggc gcgccaggct ggcgattcgc
     9841 gatgtcgagg acaagatccg cgccatcgaa gccgccgaca gcgcccgccg ccagcggaca
     9901 acgccggaac gggaattcac gctggcactg gaggcggggc aggcgctcaa gggcacactg
     9961 accctcagct atcgcgttgg cggcgcgtcc tggcgcccgg cctatgatgc gactctggag
    10021 acaggggagg gtggcaaggc ggctggcctg aagctgacgc ggcgcgcgct cgtgacccag
    10081 cggaccggcg aggactggag cgatgtggcg atgacgctgt ccacgcaact gcgcagcggc
    10141 gcctaccggc tgcg
//
LOCUS       DAOMXN010000075        10108 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_3531_length_10108_cov_12.012335, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000075 DAOMXN010000000
VERSION     DAOMXN010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 10108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10108
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3531_length_10108_cov_12.012335"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="PK812_07100"
     CDS             <1..220
                     /locus_tag="PK812_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HRJ69359.1"
                     /translation="GSEPALIARERQRRAVAEARDGLVAARALARRAPELCAEEVRLA
                     ERALERLVGRNDVEAVLGAIFSRFCIGK"
     gene            342..2225
                     /gene="mnmG"
                     /locus_tag="PK812_07105"
     CDS             342..2225
                     /gene="mnmG"
                     /locus_tag="PK812_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HRJ69360.1"
                     /translation="MRDDGWNFDVLVIGAGHAGVEAAAAAARLGARVGLVTLRRDNLG
                     VMSCNPAIGGLGKGHLVREVDALDGLMGRVADRAGIQFRLLNRRKGPAVRGLRAQADR
                     KLYRRAMSDAIGQTAGLSVIEAEVADLIVDGHAVAGIRTADGRDMRAGAVVVTTGTFL
                     RGVIHIGERRMPAGRVGEAPAVRLAERFADIGFALDRLKTGTPPRLDGRTIDWASLDM
                     QEGDDPPEPFSALTDRITNPQVRCGITRTTDDVHRAIRDNLHRSAMYSGGIRGRGPRY
                     CPSIEDKIVRFGEREGHQIFLEPEGLDDVTVYPNGISTSLPEDVQQRVVAGIPGLQRA
                     VILQPGYAIEYDFIDPRELVPTLETKRVARLFLAGQINGTTGYEEAAAQGIVAGLNAA
                     RRAGGGDGVMFERAQSYIGVMIDDLVTRGVSEPYRMFTSRAEYRLSLRSDNADQRLTP
                     LGLKLGCVGGERAARFAAWHGALAEGRHALEARGITPTEGARFGLELNRDGQRRTAFD
                     LLSLPSLEFSDLIRIWPDLDSVSDKVREALTTESRYAVYLARQHADILRTERDRAQVI
                     DLDFRYQGLPGLSNELAIKLETIRPRTIDQASRIEGMTPAALALIAAHARKLERDRSG
                     LVA"
     gene            2222..2905
                     /gene="rsmG"
                     /locus_tag="PK812_07110"
     CDS             2222..2905
                     /gene="rsmG"
                     /locus_tag="PK812_07110"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HRJ69361.1"
                     /translation="MSTGTAALLAQALVEDRRRFVDLRPVSRETLRDLDLYVAALTRW
                     QASINLVGPATLPQLWTRHFLDSVQLMDHAPAHALRWVDLGSGAGFPGLVIALALKGR
                     AGATVTLVESNGKKAGFLRAVAREISAPADVRHGRIEDVVPRLASTDVVTARALAPLT
                     QLFAWVQPLLKSGAIALFPKGRDLDKERADAARYWDFSGEVLPSVVTEESHILRIHRL
                     TRRSGQSIP"
     gene            2935..3729
                     /locus_tag="PK812_07115"
     CDS             2935..3729
                     /locus_tag="PK812_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020179470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HRJ69362.1"
                     /translation="MKARILAIANQKGGVGKTTTAINLGTALAAIGERVLIVDLDPQG
                     NASTGLGIEQKARRLSTYDVLLGDATFAEAIQPTSVPRVFVAPSTMDLLGAELELAPA
                     RDRTQRLRNALQRHQEHEAEPFTYVLIDCPPSLNLLTVNAMAAADAVLVPLQCEFFAL
                     EGLSQLLKTVEQVRSTLNPNLIIHGVVLTMFDPRNNLAGQVISDVRQFLGEKVYQTVI
                     PRNVRVSEAPSHGKPVLLYDLKCAGSQAYLRLASEVIQRERSLRAA"
     gene            3788..4657
                     /locus_tag="PK812_07120"
     CDS             3788..4657
                     /locus_tag="PK812_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRJ69363.1"
                     /translation="MADETRQRLGRGLAALIGDVGDENAVIERNRRQRRIAIDLIRPN
                     PHNPRRVFSETELEELAASIREKDVLQPIIVRSVAGVPNEYEIIAGERRWRAAQRAGL
                     HDIPAVQVEADDRQAMEIAIIENIQRSDLNPLEEARGYESLIAQFGYSQSDLAKVVGK
                     SRPHVANTLRLARLPEPVKALVNEGKVSAGHARALLAVADPETVAQRIVAQGLTVRDV
                     EKLAQREAEGGEAPKPAPPQKDADTRALEKALGDVLGLAVSIDHRKAGGALTIRYRSL
                     EQLDGLCRRLRGA"
     gene            complement(4693..5706)
                     /gene="holA"
                     /locus_tag="PK812_07125"
     CDS             complement(4693..5706)
                     /gene="holA"
                     /locus_tag="PK812_07125"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HRJ69364.1"
                     /translation="MAVIRSADLPRFLASGWKGYRMFLVHGSDEGLVRETTAQLLSSA
                     GGAQPDPLNMVVLEGDQIAQDPARLADELGTFGMFGGTRVIHVRNAGRVPPAVATTVF
                     ADPVENTVLALEAGELRTGSPLRTLAEKAPGVAAIACYADTTQAIQRLIADTLQAHGL
                     TIAADARDLLAQALGSDRALSRGEIEKLALFASGEKTVTAAMVTAIVSDAGKHEVSAL
                     IDRAFAGQIREIEAQAHRFFAAGTNPAAIFTQAIGHVLLLRRALRNGNAELVAKQARL
                     HFSREPAFHRAAGLWTESRLERALRLLSDAVLQSRKSARMAETLTVRTLWAISRLAGG
                     DGG"
     gene            complement(5716..6222)
                     /locus_tag="PK812_07130"
     CDS             complement(5716..6222)
                     /locus_tag="PK812_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69365.1"
                     /translation="MLARLARPTIALALALGLGGCFQPLHGGALSGSADVLAQIEVMP
                     ITGHLGHQVKSELDFVLNNGRPPENPQYRLTVRTNPSVGSVVADSVGGRPQVMTLVVS
                     ANYQLVAIKDGRSISFGTATSNASFDRTQQRFATTRATVDAEIRSAKVLAEQIKARII
                     PDLFAPKS"
     gene            complement(6237..8837)
                     /gene="leuS"
                     /locus_tag="PK812_07135"
     CDS             complement(6237..8837)
                     /gene="leuS"
                     /locus_tag="PK812_07135"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HRJ69366.1"
                     /translation="MRATRYNTRAAEPKWQKIWDERQTFVTRNDDVRPKYYVLEMFPY
                     PSGRIHMGHVRNYAMGDVVARYKRAKGFSVLHPMGWDAFGMPAENAAMEKKSHPKTWT
                     YANIATMKAQLKSMGLSLDWSREIATCDPAYYRHQQAMFLDFLESGLVARKTSKVNWD
                     PVDQTVLANEQVIDGRGWRSGALVEQRELTQWFLKITDYAQDLADALDRLDRWPEKVR
                     LMQKNWIGRSEGLMVRWALQNAPAGQSEVEIYTTRHDTLFGASFIALAPDHPLARAAA
                     GQDGKLAAFIDECRRMGTSVAVLETAEKKGYDTGIRAVHPFDPAWTVPVYVANFILMD
                     YGTGAIFGCPAHDQRDLDFANAYGLPVTPVICPPGRTAAEVATAFDRSAPAFVDDGVL
                     MNSRFLDGLAVDAAMEDVARRLEQARLSGAPVGRRRVNFRLRDWGISRQRYWGCPIPV
                     IHCEACGVVPVPKKDLPVSLPEDVTFDVPGNPLDRHPTWKHVSCPQCGARARRETDTM
                     DTFVDSSWYFARFTDPANSDAPTTRAAVDRWLPVDQYIGGIEHAILHLLYSRFFTRAM
                     RKTGHAGLDEPFDGLFTQGMVVHETYRAADGTWVEPAMVEIVTGEGVRTARHRDTGAE
                     IAIGSIEKMSKSKKNVVDPDDIIETYGADTARWFMLSDSPPERDVIWSEDGVQGAGRF
                     VQKVWRIVCETADLQPGPTGDRKASAVLKAAHRATKAVGEDIERLRFNRCVAHVHELS
                     NAIQAALAEGKENSAFVREAVEMMIGLFAPMMPHLAEECWAELGHDALVAESSWPRFD
                     PDLTVDSTIVLPVQINGRKRADVEVAAEAGPAEVEAATLALEAVRRALEGRSPKKVIV
                     VPRRIVNIVA"
     gene            8962..9630
                     /locus_tag="PK812_07140"
     CDS             8962..9630
                     /locus_tag="PK812_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HRJ69367.1"
                     /translation="MDDKDEAVERLNSVRRRIRSAEADFGRSAGSVTLVAVSKTFPTE
                     AIRPALAAGQRVFAENRVQEAQAKWPALRAEHADVTLHLIGSLQTNKAQEAVETFDVI
                     EVVDRPKLARALKAAMDRTGRRPAILVQVNTGEEPQKGGVAPGAADALVAECRALGLN
                     VAGLMCIPPEGEPPSPHFALLEKIARRNGLGVLSMGMSADFPAAIQCGATHVRVGSAI
                     FGQR"
     gene            complement(9657..10106)
                     /locus_tag="PK812_07145"
     CDS             complement(9657..10106)
                     /locus_tag="PK812_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69368.1"
                     /translation="MRLPCRIGRNGATVFKREGDGKSPRAILLARRGWWRTDRRLPPR
                     GGVPLRPARKELGWCDAPGHACYNRLVRLPFEHSHETLCRDDRQYDVVLELDWNARPR
                     IQGRGSAIFLHVMAEAGTGTAGCIALSPGHIDRLLACLGPRTRIRIV"
BASE COUNT         1762 a         3202 c         3439 g         1705 t
ORIGIN      
        1 cggcagcgag ccggcgctga ttgcacggga aagacagagg cgcgccgtcg ctgaggcgcg
       61 ggatggcctt gttgccgcca gggccctggc tcgccgggcg ccggagctgt gcgcggagga
      121 ggtccgtctg gcggaacggg ccctcgaacg cctcgtggga cgtaacgatg tcgaggccgt
      181 cctgggggcc atcttctccc gcttctgcat tggcaagtag gcgtttcacg tgaaacaggc
      241 gttcgattct gactcgaaac aagcggcaac gtgggcgtcg gcgcgctgtc gcgaagcaaa
      301 gactttgacg aggcaggtca gttcttctat gggcgaaatc catgcgggat gacggttgga
      361 acttcgatgt cctggtgatc ggcgcggggc atgccggtgt cgaggccgcc gccgccgccg
      421 cacgactcgg cgcgcgtgtt ggtctggtca cgctccggcg ggacaatctc ggcgtgatgt
      481 cctgcaatcc cgcgatcggt ggtctcggca aaggtcatct cgtgcgcgag gtcgatgcgc
      541 tggacgggct gatgggccgc gtggcagatc gcgcaggcat tcagttccgg ctgttgaacc
      601 gacgcaaggg tccggcagtg cggggactgc gggcacaggc cgaccgcaaa ctctatcggc
      661 gtgcgatgag cgatgcgatt gggcagaccg ccggattgtc tgtgatcgag gctgaggttg
      721 cggacctgat tgtcgacggc catgcggtgg ccggaatccg gacggcggat ggtcgcgaca
      781 tgcgtgccgg agccgttgta gtgacgaccg gcacgtttct gcgcggcgtg atacacatcg
      841 gtgagcgccg catgcctgcc ggacgtgtgg gcgaagcgcc ggcggtgcgt ctggccgagc
      901 ggttcgcgga catcggattc gctctggacc ggctcaagac cgggaccccg ccaagacttg
      961 atgggcggac catcgactgg gccagccttg acatgcagga gggggacgat ccgccggagc
     1021 cattctcggc gctgaccgac aggatcacca atccccaggt tcggtgcgga attacgcgta
     1081 cgacggacga cgtgcatcgc gcgatccgcg acaacctgca tcgctccgcc atgtattccg
     1141 gcggaatccg gggtcgcgga ccccgctact gcccctccat cgaggacaag atcgtccggt
     1201 tcggtgagcg cgagggacac cagatcttcc tcgagccgga ggggctcgat gacgtgacgg
     1261 tctatccaaa cggcatatcg acatcgctgc ccgaggatgt gcagcagcgc gtcgtggccg
     1321 gcattcccgg cctccagcgg gccgtgatcc tgcaaccggg ctatgccatc gaatacgatt
     1381 tcatcgaccc ccgcgaactg gtgccgacgc tggaaaccaa gcgggtggcc cggttgttcc
     1441 tcgcgggtca gatcaatggc accacgggat acgaagaagc ggcggcacag gggatcgtgg
     1501 caggtctcaa cgcggcgcga cgggcggggg gcggcgacgg cgttatgttc gagcgggcgc
     1561 aatcctacat cggcgtgatg atcgacgatc tcgtcacgcg cggagtcagc gaaccctatc
     1621 gtatgttcac ctcgcgtgcg gaatatcggc tgagtctgcg atcggacaat gcggaccagc
     1681 gcctgacgcc gcttggactg aaactgggct gcgtcggtgg ggaaagggca gcgaggtttg
     1741 cggcctggca cggcgcgctc gcggagggac ggcacgccct ggaagcgcgc gggattactc
     1801 cgaccgaagg cgctcgattc ggactcgaac tgaatcgtga tgggcagcgg cgaaccgcgt
     1861 tcgacctgct ctcgctgccc agccttgagt tcagcgatct gatccggatc tggcctgacc
     1921 tggactccgt gtcggacaag gtgcgtgagg cgctgaccac ggaatcgcgc tacgccgtct
     1981 atttggcgcg gcaacatgcg gacatcctaa ggactgagcg cgatcgcgcc caggtcatcg
     2041 atctggattt ccgctatcaa ggcctgcctg gattgtccaa cgagctggcc atcaagctcg
     2101 aaacgattcg accacgaacc attgatcagg cgagtcgaat cgagggcatg acgccggcgg
     2161 ctctggcgtt gatcgcggcg catgcccgga agcttgagcg ggaccgctcc gggctcgtgg
     2221 catgagcacc ggaacggcgg ctctcctggc gcaagccctg gtcgaggacc ggcggcgctt
     2281 tgtcgatctc aggccggttt cacgtgaaac gctgagagac ctcgatctct atgtcgcggc
     2341 gctgacgcgc tggcaggcat caatcaacct ggtcgggccg gcgacccttc cgcagctttg
     2401 gacccggcac ttcctcgatt cggtgcagct catggatcat gctccggcgc atgccttgcg
     2461 ctgggtcgac cttgggtccg gggcgggatt cccgggactg gtgatcgcgc ttgccctgaa
     2521 gggcagggcc ggcgcaaccg tcaccttggt cgagagcaat ggcaagaagg ctggatttct
     2581 gagggcggtg gcgcgtgaaa tcagcgctcc cgccgacgtg cggcacggcc ggatcgagga
     2641 cgttgtcccc aggcttgcgt ccactgacgt cgtcacggcg cgggcgctgg cgccgctcac
     2701 gcagttgttc gcctgggtcc aaccgctgtt gaaaagcggt gcaatcgcgc tgtttcccaa
     2761 ggggcgcgat ctggacaagg aacgggcaga tgccgcaaga tattgggact tctcgggcga
     2821 agtcctacca agtgttgtaa ccgaggagtc ccatatcctt cgcatccacc ggttgacgcg
     2881 tcgatcagga cagtcgatac cctgagccgg acaagcagaa acggacccgc gccgatgaaa
     2941 gccagaatcc ttgcaatcgc caatcagaag ggcggcgtcg gaaagacgac cacggccatc
     3001 aatctcggga cggcgctggc ggcaattggc gagcgggtgc tgatcgtcga tctcgatccg
     3061 cagggcaatg cctcgacggg ccttggcatc gagcagaagg cgcggcggtt gtcgacctac
     3121 gacgtgttgc tgggcgatgc gacctttgcc gaggccatcc agccgacctc agttccgcgg
     3181 gtcttcgttg ctccctcgac catggatctg ctgggtgccg aactcgagct tgcaccggcg
     3241 cgagaccgca cgcaacggtt gcgcaatgcg ctccagcgcc accaggagca cgaggccgag
     3301 cccttcacct atgtgttgat cgattgcccg ccctcgctca acctgctgac ggtcaacgcc
     3361 atggccgcgg ccgatgcggt gctggtgccg ctgcaatgcg agttcttcgc tcttgaaggc
     3421 ctgagccagt tgctcaagac cgtcgaacag gtgcgctcaa cgctcaatcc gaacctgatc
     3481 atccatggcg tggtgctgac catgttcgat ccacgcaaca accttgccgg acaggtcatt
     3541 tccgatgtgc gtcagttcct tggcgagaag gtctaccaga cggtgattcc gcgcaatgtc
     3601 cgggtgtccg aagcgccttc gcacggcaag ccggtgctgc tctatgatct gaaatgcgcc
     3661 ggcagccagg cgtatctgcg ccttgcgtcc gaagtcatcc agcgcgagag gtcactgcgc
     3721 gccgcctgac tccggcccgg agcctcctgc ccaacgattc caaagcattg attcggggac
     3781 aaaacaaatg gcggacgaga cgcggcagag gctggggcgt ggacttgcgg cgctgatcgg
     3841 cgacgttggc gacgaaaacg ccgtgatcga gcgcaaccgg cgccagcggc gcatcgccat
     3901 cgacctgatc cggcccaatc cccacaatcc gcggcgggtg ttcagcgaga ccgaactcga
     3961 ggagcttgcc gcttcgatcc gcgagaagga cgtgttgcag ccgatcatcg tgcggtcggt
     4021 ggcgggggtg ccgaacgagt acgagatcat cgccggcgag cggcgctggc gcgcggcgca
     4081 gcgtgccggg ctgcatgaca ttccggcggt ccaggtggag gcggacgacc gccaggcgat
     4141 ggaaatcgcc atcatcgaga acatccagcg ttcggacctc aatccgctgg aagaagcgcg
     4201 tggctacgaa agcctgatcg cccagttcgg ttacagccag tccgacctcg ccaaggtggt
     4261 gggaaagagc cggcctcatg tggccaacac gttgcggctc gcccgtctgc cggaaccggt
     4321 gaaggcgctg gtgaacgagg gcaaggtgtc tgccggccat gcacgagcgc tgctggcggt
     4381 cgccgatccg gagaccgtgg cgcagcgcat cgtcgcgcag ggcctgacag tgcgcgatgt
     4441 ggaaaagctc gcccaaaggg aggccgaggg cggagaagcg cccaagccgg cgccgccgca
     4501 aaaggatgcg gacacccggg cgctggagaa ggcactcggt gacgttctgg gccttgcggt
     4561 ttcgatcgat catcgcaagg ccggcggggc gctgacgatc cgctaccggt cgctggagca
     4621 actggacggc ctgtgtcggc gtctgcgcgg cgcctgaccg gcaccttcgc cataccgccg
     4681 tcgggagggc cgctatccgc catcgcctcc ggcaagtcgg ctgatggccc atagcgtgcg
     4741 cacggtcagg gtttccgcca tgcgggcgga cttgcggctc tgcaggacgg catccgacag
     4801 cagccgcagg gcgcgctcaa gccgcgattc tgtccacagt ccggcagcac ggtggaaggc
     4861 aggctcgcgc gagaagtgca ggcgcgcctg cttggccacc agctcggcat tgccgttccg
     4921 aagcgcgcga cgcagcagca gcacatggcc gatggcttgc gtgaagatgg ccgcgggatt
     4981 ggtgccggcg gcaaagaatc gatgggcttg cgcctcgatc tcgcggatct gcccggcaaa
     5041 agcccgatcg atcagcgcgc tgacctcgtg cttgccggca tctgagacaa tggccgttac
     5101 catggctgcg gtaacggttt tctcgccgct ggcaaagagc gccagtttct cgatctcgcc
     5161 gcgcgacagc gcccggtcgc tgcccagcgc ctgggcaagc aggtcgcggg catcggctgc
     5221 gatcgtaagg ccatgggctt gcagcgtatc ggcgatgagg cgctgaatgg cctgagtcgt
     5281 atcggcatag caggcaatgg ccgcaacgcc cggagctttc tccgccaggg tgcgaagggg
     5341 cgatcccgtc cgcagctcgc cggcctcgag cgcaaggacg gtgttctcca cgggatcggc
     5401 gaagaccgtc gtcgcgacgg ccggcggcac gcggccggcg ttgcgcacat gaatgacgcg
     5461 cgtcccgcca aacatgccga aggttcccag ttcatccgcg agccgggccg ggtcctgcgc
     5521 gatctggtcc ccttccagaa caaccatgtt caggggatcg ggctgggcgc cgcctgcgct
     5581 ggagagaagc tgggccgtcg tctcgcgcac cagcccctcg tccgaaccat gaaccaggaa
     5641 catgcggtag cccttccagc ccgaggcgag aaagcgcggc agatcagcag accggatgac
     5701 ggccatcggc tccgatcagc tcttgggggc gaacagatcg ggaataatgc gcgccttgat
     5761 ctgttccgcc agcaccttcg cggatctgat ctccgcatcg acggtggcgc gcgtcgtggc
     5821 aaaacgctgc tgcgtccggt cgaaggatgc gtttgaggtg gcggttccga acgaaatcga
     5881 gcgaccatcc ttgatcgcga caagctggta gttggcggag accaccaagg tcatgacctg
     5941 gggcctgccg ccgacagaat cggcaaccac gctgcccacg gacgggttgg tcctgaccgt
     6001 tagcctgtac tgcgggttct cgggcggcct gccgttgttc aggacgaaat ccagctccga
     6061 cttcacctgg tgacccaggt gccctgtgat gggcatgacc tcgatctggg ccagcacatc
     6121 ggccgaaccg gacaatgcgc cgccatgcag cggctggaag caaccgccca ggccaagtgc
     6181 gagggcgagg gcgatcgtcg gtcgggcaag gcgagcgagc atggcgcgtt tccctttcag
     6241 gcgacgatat tgacgatgcg tctgggcacg acaatgacct ttttcgggct acgcccttcc
     6301 agggcccgcc gcaccgcctc cagagccagc gttgccgcct cgacttccgc cggcccggcc
     6361 tcggccgcaa cctcgacgtc ggcgcgcttg cggccattga tctgcaccgg aagcacgatc
     6421 gtcgagtcga ccgtcaggtc gggatcgaaa cgcggccaag aggattccgc caccagcgcg
     6481 tcatggccca gttccgccca gcattcctcg gccagatggg gcatcatcgg cgcgaacagg
     6541 ccaatcatca tttcaaccgc ctcgcgcacg aaggccgaat tctccttgcc ctcggccaga
     6601 gcggcctgga tggcgttgga cagttcatgc acatgggcga cgcagcgatt gaaacgcagg
     6661 cgctcgatat cctcgccgac cgccttcgtc gcccggtgcg cggccttgag gacggctgaa
     6721 gccttgcggt cgcccgtcgg tcccggctgc aggtccgccg tctcgcagac gatgcgccag
     6781 accttctgca cgaagcgtcc cgcgccttgc accccatcct ccgaccagat gacatcgcgc
     6841 tcgggaggcg aatccgacag catgaaccag cgggccgtat cggcgccgta agtctcgatg
     6901 atgtcgtccg gatcgaccac gttcttcttc gacttcgaca tcttctcgat ggagccgatg
     6961 gcaatctcgg cgccggtgtc gcgatggcgt gccgtacgga cgccctcgcc cgtgacgatc
     7021 tccaccatgg ctggctcgac ccaggtgcca tcggcagccc ggtacgtctc gtgcaccacc
     7081 atgccctgcg tgaacaggcc gtcaaacggt tcgtccaacc cggcatggcc ggtcttccgc
     7141 atggctcttg tgaagaagcg ggaatagagc aggtgcagga tcgcatgctc gatgccgccg
     7201 atatactggt cgaccggtag ccagcggtcg acggcggcgc gggtggtcgg cgcatcggaa
     7261 ttggccgggt cggtgaagcg cgcgaaatac caggacgaat cgacaaacgt gtccatggtg
     7321 tcggtttcgc gtcgtgccct tgcgccgcac tgcgggcagg acacgtgctt ccaggtcgga
     7381 tgccgatcca gtggattgcc gggcacgtcg aaggttacgt cctccggaag gctcaccggc
     7441 agatccttct tcggcaccgg aacgacgccg caggcctcgc aatggattac cggaatcggg
     7501 caaccccaat agcgctggcg ggatatgccc cagtcgcgca ggcggaaatt gaccctgcgc
     7561 ctgccgacgg gggcgccgga aagcctcgcc tgctcaagcc gccgcgccac gtcttccatg
     7621 gccgcatcga cggcaaggcc atcgagaaaa cgcgagttca tcagcacgcc gtcatcgacg
     7681 aaggccggcg ccgaacggtc gaacgcggtg gccacctcag cggccgtcct gcccggcggg
     7741 cagatcaccg gcgtcaccgg caggccgtag gcgttggcga aatcaagatc gcgctggtcg
     7801 tgggcagggc agccgaaaat ggcgccggtg ccgtagtcca tcaggatgaa attcgcgaca
     7861 tagaccggaa cggtccaggc cggatcgaac ggatgaaccg cgcggattcc ggtgtcgtag
     7921 cccttcttct ctgccgtctc cagtaccgcg accgatgtgc ccatgcgccg gcactcgtcg
     7981 atgaaggcgg caagcttgcc atcttgcccg gcggcggccc tggccagcgg atgatccggc
     8041 gccagcgcga tgaagctcgc gccgaacagc gtatcgtggc gtgtggtgta gatctcgacc
     8101 tcggactgtc cggcgggggc gttctggagc gcccatcgca ccatcagccc ctccgaacga
     8161 ccgatccagt tcttctgcat cagccggacc ttctccggcc agcggtccag ccggtcgagc
     8221 gcgtcagcaa ggtcctgggc gtaatccgtg atcttcagga accattgggt gagttcgcgc
     8281 tgttccacca gcgcgcccga gcgccagcca cgcccgtcga tgacctgctc gttggcaagc
     8341 acggtctggt cgaccgggtc ccagttcacc ttggaggtct tgcgcgccac gagcccggat
     8401 tcgaggaaat ccaggaacat ggcctgttga tgccgataat aggcgggatc gcaggtggcg
     8461 atctcgcgac tccagtccag agacaggccc atcgacttga gctgcgcctt catcgtcgcg
     8521 atgttggcgt aggtccaggt cttgggatgc gacttcttct ccatcgcggc gttctcggcc
     8581 ggcatgccga aagcgtccca gcccatggga tgcagcacgg agaagccttt cgcccgcttg
     8641 tagcgcgcca ccacgtcgcc catggcgtag ttgcggacgt ggcccatgtg aatgcgcccc
     8701 gagggataag gaaacatctc gagcacgtaa tacttcggcc gcacgtcgtc gttgcgggtc
     8761 acgaaagtct ggcgttcatc ccagatcttc tgccatttgg gctcggcggc ccgggtgttg
     8821 tatctcgtcg cgcgcatatc gaaatcatcg gtccgtgcag gggtcccttg gacaacatcc
     8881 ggtcgggccg gtcaaccgca ctgtgaggca aaaccggcgg tccctgcgat gcgccggcgg
     8941 cactttggga gatcatgaga cgtggacgac aaggacgagg ccgtcgaacg gctcaacagc
     9001 gtgcgccgtc gcattcgcag tgccgaggct gacttcgggc gcagcgccgg atcggtcacg
     9061 ctggtggcgg tgtcgaaaac ttttccgacc gaggccatcc gccccgcgct ggcggccggc
     9121 cagcgcgtct tcgcagagaa cagggtgcag gaagcgcagg cgaaatggcc ggcgctgcgg
     9181 gcggaacatg cggacgtcac gctgcatctc atcggctcgc tgcagacgaa caaggcacag
     9241 gaggcggtcg agacgttcga tgtgatcgaa gtggtcgacc gaccaaaact ggcgcgggcg
     9301 ctgaaagcgg ccatggacag gaccggccgg cggccggcca tcctggtgca ggtcaatacc
     9361 ggcgaggagc cgcagaaggg gggcgtggcg ccgggcgcgg cggatgccct ggtggcggaa
     9421 tgccgggcgc ttggcctgaa tgtcgcaggg ttgatgtgca tcccgcctga aggcgagccg
     9481 ccctcaccgc atttcgccct gctggagaag attgcccggc gcaacgggct tggtgtgctc
     9541 tctatgggca tgagcgcgga ttttcccgca gctatccagt gcggcgccac gcatgtgcgc
     9601 gtcggcagcg cgatcttcgg gcagcggtga ggatcggcgg ggcgggcagg ttccggtcag
     9661 acaatccgga tgcgcgtgcg cggcccgagg caggcgagca ggcggtcgat gtgccccggc
     9721 gacagcgcga tacagcctgc cgtgccggtt ccggcctcgg ccatgacatg caggaagatg
     9781 gcgctgcctc gcccctggat gcgcgggcgc gcgttccagt ccagttcgag cacgacatca
     9841 tactggcggt cgtcgcgaca cagcgtttcg tggctgtgtt cgaagggcag ccgcacgagc
     9901 cggttgtagc aggcgtggcc cggagcgtcg caccagccca gctccttgcg cgcaggcctc
     9961 aggggaaccc cgcctcgggg aggcaggcgg cggtcggtgc gccaccagcc gcgccgggcc
    10021 agcaggatgg cgcggggcga tttcccgtca ccctcgcgct tgaagaccgt ggccccgttg
    10081 cggccgatcc ggcagggcag gcgcaggg
//
LOCUS       DAOMXN010000076        10048 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_3551_length_10048_cov_13.297408, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000076 DAOMXN010000000
VERSION     DAOMXN010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 10048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10048
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3551_length_10048_cov_13.297408"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /gene="rimI"
                     /locus_tag="PK812_07150"
     CDS             <1..522
                     /gene="rimI"
                     /locus_tag="PK812_07150"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HRJ69369.1"
                     /translation="PMWFTLWWPFWWWSGAPKLRVAEAGDAQALSLIHAQGFAHPWSA
                     MTIESMLAERTIASHVIAASSPEGFIIARCVADEAEIITVAVARASRGKGYGRRLLDA
                     SLDELVRRRVRRVFLEVEAENAAAIHLYRQAGFERIGQRRGYYRQADGSRRDAVTMAL
                     NLESRPLVPALDG"
     gene            572..1399
                     /locus_tag="PK812_07155"
     CDS             572..1399
                     /locus_tag="PK812_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRJ69370.1"
                     /translation="MNLAGRAKVGLKIAALAPAFALGLPAQWLALRSGGALPRHLPVT
                     FHRYITRVLGIRARIDGTPCDGPVLIVSNHTSWLDIVVLGSLMPLSFIAKSEVGAWPL
                     FGTLARLQRTVFVDRQKKTATGAASRSIGRRLAGGDAMVLFAEGTTSDGGRVLPFRSA
                     LLGAARAALEDERATVLVQPLSVIYTHRDGLPLGKAGRPFLAWYGDMDLVPHLAAILA
                     GGPIDVAVSFGEPIPFAPNSNRKVIAALAETTVRMAARKAVTGRAGFDPGRETAKEE"
     gene            1399..2811
                     /gene="miaB"
                     /locus_tag="PK812_07160"
     CDS             1399..2811
                     /gene="miaB"
                     /locus_tag="PK812_07160"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HRJ69371.1"
                     /translation="MRRSDPETSGAEAAEPAPAPKRVHIKSFGCQMNVYDAQRMADLL
                     APEGFAETDRPEDADLVILNTCHIREKAAEKVYSELGRLRALKQNRAEAGLETAIAVA
                     GCVAQAEGGEILKRAPVVDLVVGPQSYHRLPHLLAQARAKRRAVDTDFPEEDKFEHLP
                     RASSEKTRARGVSAFLTVQEGCDKFCTFCVVPYTRGAEVSRPVDRIVAEARDLAAAGV
                     REITLIGQNVNAFHGAGPDGHAWNLARLLEAVASVGGVERLRYATSHPRDMDDDLIAL
                     HRDLPTLMPYLHLPVQAGSDRVLKAMNRQHTADDYRRLIERVRTARADIALSSDFIVG
                     FPGESDADFEDTMRLVRDIGFASAFSFKYSARPGTPASDMAGQVPEAVKAERLALLQA
                     LLNAQQQAFNAAMTGRVVDVLFEKKGRHPGQVAGKSPYLQAVQIDGPESLIGSVASVE
                     ITGLATYSLFGRLRMREAAA"
     gene            2808..3824
                     /locus_tag="PK812_07165"
     CDS             2808..3824
                     /locus_tag="PK812_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRJ69372.1"
                     /translation="MSLESGDVRQARDDEAQSTLTLEDNRLAGLVFGQYDQNLAHLER
                     RLAITAHVNGNHVVLRGPRDATEQAKRVIRAVYAQVKSGRHITQGDLDGAIAEAALQG
                     TLFPQADAGKGGFSQIVTRKRGPVKARNAAQDAYLRALQRDELVFAEGPAGTGKTWLA
                     VGQAVQLLEQGKVERLVLSRPAVEAGERLGFLPGDMKEKVDPYLRPIYDALYDFMDGR
                     AVERGLQTGMIEVAPLAFMRGRTLSNAAILLDEAQNASSMQMKMFLTRLGEGSRMIIT
                     GDPSQIDLPPGQKSGLIEAVAILDGVAGIGRVVFGHADVVRHDLVRRIVKAYEDAAGE
                     VRKG"
     gene            3821..4312
                     /gene="ybeY"
                     /locus_tag="PK812_07170"
     CDS             3821..4312
                     /gene="ybeY"
                     /locus_tag="PK812_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HRJ69373.1"
                     /translation="MTVAVIEIIVADDRWLEVPGVESTAEVSVRAAFAASGLALLPSA
                     EASVLLTDDDAVAELNRKWRGKPTATNVLSFPAAAPEALARSPVIGDIALAYQTCRRE
                     AEEQGKPLADHLAHLVVHGALHLVGYDHEGEAEAERMEALEVRVLAGLGIADPYVGTE
                     AAS"
     gene            4309..5259
                     /locus_tag="PK812_07175"
     CDS             4309..5259
                     /locus_tag="PK812_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRJ69374.1"
                     /translation="MSQSEQSDRPRDSWLERVKAALGLTESGSIREDLADALQEPELA
                     TDFSPKERMILVNVLGMRNVRVSDVMTPRARMIGVGEDATLSELFALFQNESHARLPV
                     YGDTPDDPKGMVHIRDFLAWLGGDPGAPLAGAEAKLKSRLRDAPLLRPILFVPPSMAA
                     LDLMVKMQAKRLHMALVINEYGETDGIVTMEDLVEAIVGDIDDEHDMPEPPSFAVIAP
                     GKVEAMASASLEEVSEVLGIALESDTSGVEVDSLGGLVTGLAGRVPQVGEKIEGPGSL
                     IFEIVEADPRRIRRLIIHGPAGLTASGKSGSDSPPQIAGS"
     gene            5256..6926
                     /gene="lnt"
                     /locus_tag="PK812_07180"
     CDS             5256..6926
                     /gene="lnt"
                     /locus_tag="PK812_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HRJ69375.1"
                     /translation="MIDRVIDISHAVILSEGWRRRMMAFAAGALGALAAPPVGAFPVL
                     ALSFPIAIWLLDGCTAAPGRYSAPVLRQAAGDGWWFGFGYFLAGLWWIGAAFLVETDE
                     FAWAIPLGVIGLPAALAMFVALGFALARLLWSPGLGRIFAFAFGLGVAEWLRHWLFTG
                     FPWNGFGHGLGNWLVTAQAASLIGAEGLAMVSLVVFALPATVVDAASRRPWRATLFGL
                     SVLGVLVAFGVTRLLLAGGLAAQVGPEHVVDGVRLRLVQPNISQRDKHTQRDGHMVLT
                     RLLTLSDRSTGPTATGMADSSHLVWPESPFPFVLSRDQRAMAELARQLPPKATLLTGA
                     VRLEEGAARERRFFNTLHVITGRDGVLRSYDKQHLVPFGEYLPLEAVLSTLGLRRFVH
                     APGAFAVGSPRQLLEVAGLPPVLPLICYEAIFPLEILGRTPRPGLLLNITNDAWFGET
                     FGPHQHFAQARLRAIEFGLPLVRAANTGISAVVDGYGRQVATIPLGAEGIADAPLPKP
                     LTTTAYWSHGNELRYFFLLLSMVLAVGIGPISNRFRKRTQARTASKHQ"
     gene            6989..7399
                     /locus_tag="PK812_07185"
     CDS             6989..7399
                     /locus_tag="PK812_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003610697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRJ69376.1"
                     /translation="MKKSPGPVDKHVGSRVRMRRILIGMSQEKLGEALNLTFQQVQKY
                     EKGTNRIGASRLQQIAQVLGVPVTYLFEGAPQERSDGDVAFAEPRQADYSFDLLSTPD
                     GVQLAKAFMSIEDPKLRRRVVDLVNTLAESSKSG"
     gene            7586..8791
                     /gene="metK"
                     /locus_tag="PK812_07190"
     CDS             7586..8791
                     /gene="metK"
                     /locus_tag="PK812_07190"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HRJ69377.1"
                     /translation="MARSSYLFTSESVSEGHPDKVCDRISDEVVDLFFKEAAKAGMNA
                     AQVRVACETLATTNRVVIAGEVRTSLDEKTMKRKVKSAARKAIKAIGYEQDGFHWKKA
                     KIDVLLHSQSADIAQGVDSAGNKDEGAGDQGIMFGYACRETKGLMPAPIYYAHKILED
                     LTALRKKGERNCGKLGPDAKSQVTVRYENGKPVAVTQIVLSTQHLDEKLTSKDVQKIV
                     EPIIRETLADIEIAKDCKWWVNPTGKFVIGGPDGDCGLTGRKIIVDTYGGAAPHGGGA
                     FSGKDPTKVDRSAAYAARYLAKNVVAAKLADRCTIQLSYAIGVAKPLSIYVDLHGTGK
                     VDEAKLEKALGEVMDLTPRGIRTHLGLNKPIYARTAAYGHFGRKASRDGGFSWEKTDL
                     VDALKRAVA"
     gene            8798..9496
                     /gene="trmB"
                     /locus_tag="PK812_07195"
     CDS             8798..9496
                     /gene="trmB"
                     /locus_tag="PK812_07195"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HRJ69378.1"
                     /translation="MADHDTATDRAAGFFGRRKGHRLRDEQAVRLDRLLPALRIDMAR
                     PFGDPKTLFPENVEGVHLEIGFGGGEHLVDAASRHPGIGFIGCEPFVNGVAKLVAEIE
                     KRGLTNIRVHDANAADLLPLLPPASIERVYLLYPDPWPKRRQRKRRFVSPAAVKALAR
                     VLKPGGTFRFATDIDDYAGWTLARMAASPDFVWTAERADDWRAPYANWPGTRYEAKAK
                     REGRTPSYLEFRRV"
     gene            complement(9503..>10048)
                     /locus_tag="PK812_07200"
     CDS             complement(9503..>10048)
                     /locus_tag="PK812_07200"
                     /inference="COORDINATES: protein motif:HMM:NF016207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HRJ69379.1"
                     /translation="DGPFASHLPMLFEPAGGPNGRLIGHVARANPHWKLALPGRPSLA
                     IFSGPDAYVSPNWYPSKKVEGKAVPTWNYVVVQARGMLRWIDDAADLRRIVTVLTDRF
                     EAQRPAPWQVSDAPESYIAAMLRGIVGVELVIDTLAFKQKLSQNRSTEDRDGVVAGLA
                     DDPEDAGKELRALMLAKRGAS"
BASE COUNT         1667 a         3191 c         3349 g         1841 t
ORIGIN      
        1 cccatgtggt tcaccctgtg gtggccgttc tggtggtggt cgggcgcgcc gaagctgcgg
       61 gtcgcggagg ccggggacgc ccaggcgctg tcattgatcc atgcccaggg ctttgcacat
      121 ccctggtcgg ccatgaccat cgaatcgatg ctggccgaac gcaccatcgc gtcccatgtc
      181 attgcggcca gcagtcccga agggttcatc atcgcccgct gcgtggccga cgaagccgag
      241 atcatcaccg tggcggtggc cagggcatcc cgcggcaagg ggtatggacg acgtttgctc
      301 gatgccagcc tggacgagct ggtgcgccgg cgggtccgca gggtcttcct ggaggttgag
      361 gccgagaatg ccgccgccat ccatctgtac aggcaagccg gcttcgaacg catcggacag
      421 cgccgcgggt attatcgtca ggccgacggc tcccgtcgtg acgctgtcac gatggcgctg
      481 aatcttgagt cgcgcccgct ggtgcctgcg ctggacggtt aggcgcgggg tttgttagtc
      541 atgccgcctt cggtgacgtc gggatagtgt gatgaatctc gccggcaggg cgaaagtcgg
      601 gctgaagatc gcggcgctgg cccccgcctt tgcgctgggg ctgccagcgc agtggctggc
      661 gctccgatcg ggcggcgcat tgccgcgcca ccttcccgtg accttccatc ggtatattac
      721 gcgggtgctg ggaattcggg ccagaatcga cggcacgccc tgcgacgggc ctgtgctgat
      781 cgtgtccaac cacacgtcat ggcttgatat cgtcgtgctc ggctcgctca tgccgctttc
      841 tttcatagcc aagtcggaag ttggcgcctg gcccctgttc ggcacgcttg caaggttgca
      901 gcggaccgtc ttcgtggatc ggcagaagaa gacggcgacg ggggcggcca gccggtcaat
      961 cgggcggcgg ctcgcgggag gcgatgccat ggtcctgttt gccgaaggca cgaccagcga
     1021 tggcggtcgc gtcctgccct tccgctccgc ccttcttggg gccgcgcgcg cggctttgga
     1081 ggatgaacgc gccaccgtgc tcgttcaacc tctttcggtc atttataccc atcgcgatgg
     1141 cctgccgctc ggcaaggccg gtcgaccatt cctggcctgg tacggcgaca tggatctcgt
     1201 tccgcatctg gcggcgatcc tggccggagg cccgatcgat gtggcggtga gcttcgggga
     1261 gccgatcccg tttgcgccaa actccaatcg caaggtcatc gctgctctcg cagaaaccac
     1321 tgttcgcatg gcggcgcgca aagcggtgac tggtcgcgcg ggctttgatc cgggacgcga
     1381 aaccgctaag gaagagtgat gcgaagatca gatcctgaaa catccggggc ggaagcggcc
     1441 gagccggccc ctgccccgaa gcgcgtgcac atcaagtcct tcggctgcca gatgaacgtc
     1501 tatgacgccc agcggatggc cgacctgctg gcgcctgagg ggtttgccga gacggaccgc
     1561 cccgaggatg ccgatctcgt gatcctcaac acctgccata tccgcgaaaa ggccgccgag
     1621 aaggtttatt ccgaactcgg caggttacgc gccctgaagc agaaccgtgc cgaggccggc
     1681 cttgagacgg cgatcgccgt tgccggttgc gtcgcccagg ccgaaggcgg cgaaattctc
     1741 aagcgcgcgc cggtcgtcga cctggtggtc gggccgcaga gctatcatcg attgcctcat
     1801 ctgctcgcgc aggcgcgggc gaagcggcgc gcggtcgaca ccgatttccc cgaggaggac
     1861 aagttcgagc atctgccgcg tgcgagcagc gagaagacgc gcgccagagg tgtttccgcg
     1921 ttcctcacgg tgcaggaagg gtgtgacaag ttctgcacgt tttgcgtggt gccctatacg
     1981 cgcggcgcgg aggtttcgcg gcctgtcgac aggatcgtcg ccgaggcacg tgatctggcc
     2041 gcggcgggcg tgcgggagat cacgctgatc ggccagaacg tcaacgcctt ccatggcgcc
     2101 ggccccgatg gccacgcgtg gaacctggcg cggctgctcg aagcggtggc gtctgtcggc
     2161 ggtgtcgagc gtctgcggta tgcgacctcg catccccggg acatggacga cgatctgatt
     2221 gccctgcatc gcgatcttcc gacgttgatg ccctacctgc acctgccggt tcaggccggc
     2281 tcggatcgtg tcctgaaggc catgaaccgt cagcacaccg cggacgacta ccgtcgtctt
     2341 atcgaaaggg tgcgcacggc ccgggccgac attgccctgt cgtcggattt catcgttggc
     2401 ttcccgggcg agagcgatgc cgatttcgaa gatacgatgc ggttggtgcg cgacatcggc
     2461 tttgcatcgg cgttctcgtt caagtacagc gcccggcccg gcacgcctgc gagcgacatg
     2521 gccggacagg ttccagaagc ggtcaaggca gaacgcctgg cgcttcttca ggcgttgctc
     2581 aacgctcagc agcaggcgtt caatgcggcc atgacgggac gtgtcgtgga cgtgttgttc
     2641 gagaagaagg ggcgccatcc cggccaggtc gccggcaagt cgccctacct gcaggccgtg
     2701 cagatcgatg gtcctgagag tctgatcgga tcggtcgcat cggtggagat caccggattg
     2761 gcgacctatt ccctgttcgg tcgactgcgc atgagggagg cggcagcgtg agtcttgaat
     2821 ccggcgacgt ccgacaggcc cgcgacgatg aggctcaaag caccctgacc ctggaggaca
     2881 accggctcgc gggcctggtg ttcggacaat acgatcagaa cctggcgcat ctggaacgcc
     2941 ggctcgccat caccgcccat gtcaacggca atcacgtcgt gttgcgcggc ccgcgcgatg
     3001 cgaccgagca ggccaagcgg gtgatccgcg cggtctacgc gcaggtgaag tcgggacgcc
     3061 acatcacgca aggcgacctt gacggcgcca tcgccgaggc cgccctccag gggacgcttt
     3121 ttccgcaggc ggatgccggc aagggcggct tcagccagat cgtcacgcgc aagcgcgggc
     3181 cggtgaaggc gcgcaatgcc gcccaggatg cctatttgcg cgcccttcag cgggacgaac
     3241 tcgtctttgc cgaagggcca gccggcaccg gcaagacctg gcttgccgtc ggtcaggcgg
     3301 ttcagctctt ggaacagggc aaggtcgagc gtctcgtgct ctcccgtccg gccgtcgaag
     3361 ccggtgagcg cctcggcttc ttgcctggcg acatgaagga gaaggtcgat ccctatctca
     3421 ggccgatcta cgacgcgctc tacgacttca tggacggacg cgccgtggaa cgcggtctgc
     3481 agaccgggat gatcgaggtt gcgccgctgg ccttcatgcg cggccgaacg ctgtccaatg
     3541 ccgcgatcct gctggacgag gcccagaacg cctcctccat gcagatgaag atgtttctga
     3601 cccgccttgg cgaaggctcg cgcatgatca tcaccggcga cccgagccag atcgacctgc
     3661 cgcccggcca gaagtccgga ctgatcgagg ccgtcgcgat ccttgacggc gtcgccggca
     3721 tcggtcgggt cgtgttcggc catgccgacg tcgtgcggca cgacctcgtg cgccgcatcg
     3781 tcaaggccta tgaggacgcc gcgggcgaag tgcgaaaggg atgacggtgg ctgtgatcga
     3841 gatcatcgtc gcggacgatc ggtggctgga ggtgcccggc gtggagtcga cagcggaagt
     3901 ctcggtgcgc gccgcctttg cggcatcggg gctggcgctt ctgccgtcgg cggaggcctc
     3961 ggttctgctg accgacgacg atgccgttgc cgaactgaac cgcaagtggc gcggcaagcc
     4021 aaccgcgacg aacgtcctgt cctttcccgc cgctgcccct gaagcgctcg cccgcagccc
     4081 ggtgatcggt gacattgcgc tggcctacca gacatgccgg cgggaggcgg aggaacaggg
     4141 caagcccttg gctgaccacc ttgcccatct cgttgtccat ggcgccctgc acctggtcgg
     4201 gtacgatcac gaaggggagg cggaagcaga gcgcatggaa gccctggaag ttcgggttct
     4261 ggccgggctc ggcatcgccg acccctatgt cggaacggag gctgcgtcat gagccagtcg
     4321 gaacagtctg accgcccgcg cgattcctgg ctggagcgcg tcaaggccgc gctgggcctg
     4381 acggaatcgg gctcgatccg ggaggatctg gcggatgcgc tccaggagcc ggaactcgcg
     4441 accgatttct ctccgaagga gcgcatgatt ctggtcaacg tcctgggcat gcgcaacgtg
     4501 cgcgtgtctg acgtcatgac gccgcgagca cgcatgatcg gtgtcggcga ggatgcgacc
     4561 ttgtccgaac tgttcgcgct ctttcaaaac gagtcgcatg cccgtctgcc agtctatggc
     4621 gatacgccgg acgacccgaa gggcatggtg catatccggg atttcctcgc ctggctcggc
     4681 ggtgatccgg gggcaccgct ggccggcgcg gaagccaagc tgaagagccg gctcagggat
     4741 gcgccgctgc tgcgaccgat cctgttcgtt ccgccctcga tggcggcgct ggatctcatg
     4801 gtgaagatgc aggccaagcg cctccacatg gcgctggtga tcaacgagta cggcgagacc
     4861 gacggcatcg tcaccatgga ggacctggtc gaggcgattg tcggtgatat cgacgacgag
     4921 cacgatatgc ccgaaccgcc gtcgttcgcg gtgatagcgc cgggcaaggt cgaggcgatg
     4981 gcttcggcga gcctggaaga agtgtccgag gtacttggca tcgcgctgga atcggacacc
     5041 tcgggtgtgg aggtcgacag tcttggcggt ctcgtcaccg gactggccgg gcgggtgccg
     5101 caggtcggcg agaagatcga gggtcccggc agcctcatct tcgagattgt agaggccgac
     5161 ccgcgccgca tccggcgatt gatcattcac gggcctgctg gtctaaccgc ctcgggaaag
     5221 agcggctctg attcgccgcc gcagatcgcc ggttcatgat tgatcgggtc atcgacatca
     5281 gccatgccgt catactgagc gaaggctggc ggcgccggat gatggccttt gccgccggag
     5341 cgctcggagc ccttgccgcg ccgcctgtcg gtgcgtttcc cgtgctggcg ctcagttttc
     5401 ccatcgcgat ctggctcctc gatggctgca cggcggcgcc ggggcgctat tccgcgccgg
     5461 tcctcagaca ggccgcagga gacggatggt ggttcggctt cggctatttc ctcgccggat
     5521 tgtggtggat cggcgcggca tttctggttg agaccgatga atttgcctgg gcgataccgc
     5581 tgggcgtcat cgggctaccg gcggcgttgg cgatgttcgt cgccctcggc ttcgccctgg
     5641 cgcgcctgct ctggagccca gggcttgggc gcattttcgc ttttgccttc ggtctcggcg
     5701 tggcggaatg gttgcggcac tggctgttca cgggctttcc ctggaacggt ttcgggcatg
     5761 gccttggcaa ttggcttgtc accgcgcagg ccgcctcgct gatcggcgcg gaagggttgg
     5821 cgatggtctc gctggtggtc ttcgccttgc cggccacggt cgttgacgcg gcaagccgga
     5881 gaccgtggcg ggccacgctg ttcggcctct cggtgctggg tgttctggtc gcgttcggcg
     5941 tgacgcggct gttgcttgcg ggcggtcttg cggcgcaggt cgggcctgag cacgtggtcg
     6001 atggcgtcag gctgcggctc gtccagccga acatctcgca gcgggataag cacacgcagc
     6061 gtgacggtca catggtcctg acgcgattgt tgaccctcag cgatcgctcc acaggtccga
     6121 cggcgacggg gatggccgac tcctcgcacc ttgtctggcc ggagtcgcca ttcccgttcg
     6181 tcctgtcgcg cgatcagcgc gcgatggccg aactggcgcg ccagcttcca ccgaaggcaa
     6241 cccttctcac cggagcggtg cgcctggaag agggggctgc gcgtgagcgg cggttcttca
     6301 acacgcttca tgtgattacc gggcgcgacg gtgtcctgcg ctcctacgac aagcagcacc
     6361 tcgtcccgtt cggcgaatat ctgccgctgg aagcggtcct ctccacgctg gggcttcgtc
     6421 gctttgtcca tgctcccggc gcattcgccg taggctcgcc ccgccagctt ctggaggttg
     6481 ccggtctgcc gcctgtattg ccgctgatct gctacgaggc gatctttccg ctcgaaatcc
     6541 ttggccgcac gccccggccc ggattgctcc tgaacatcac caacgatgcc tggttcggcg
     6601 agacctttgg tccgcaccag catttcgccc aggcacgcct tcgcgccatc gagttcggac
     6661 ttccgctggt gcgggcggcc aataccggca tttccgccgt tgtcgacggc tacggtcgtc
     6721 aggtcgcgac cattccgctt ggggccgaag gcattgcgga cgcgccttta ccaaaaccct
     6781 tgaccacaac cgcatattgg tcgcacggaa acgagctccg gtactttttt ctgttgcttt
     6841 ctatggttct tgccgttggt attggtccta tcagcaaccg gttccggaag agaacgcagg
     6901 cgcggacggc atcgaagcat cagtgatgct tcaagcaggt tgctggaaaa cgcgttgtcc
     6961 gtgcgacacc gagctgaggg aaagaacaat gaaaaagtcg cccggtcccg ttgacaagca
     7021 tgtgggcagc cgtgtccgca tgcggcgtat cctgatcgga atgagccagg agaagctggg
     7081 tgaggccctc aatctgacat tccaacaggt ccagaagtac gagaagggca cgaaccgcat
     7141 cggcgcaagc cgcctccagc agatcgcgca ggtgctgggc gtgccggtga cctatctctt
     7201 cgagggggcg ccgcaggagc gttccgatgg ggacgtggca ttcgccgagc cgcgtcaggc
     7261 ggactattcc tttgacctct tgtcgacacc ggatggggtc caactggcca aggcgttcat
     7321 gagcatcgag gatcccaagc tgcggcgtag ggtcgtcgat ctggtcaata cgctggccga
     7381 aagcagcaag tccgggtaat tcccgctgat ttgccggttt tttgttcgga aaatcagcgg
     7441 aagtgttttt ctggcagaac gggttcggtc tttcggcttg accttgaccg gtcgagccac
     7501 tagagacgcc agcgctttca aagttgctgg acgcatcgct ccggcgcttc cccatcatca
     7561 ggcattcaac cagcggagct ccgtcgtggc gcgttcgtcc taccttttca ccagcgagtc
     7621 agtttcggaa ggtcacccgg acaaggtgtg cgaccgcatc tcggacgagg tggtcgacct
     7681 gttcttcaag gaggccgcca aggccggcat gaatgcggcg caggtgcgtg ttgcctgcga
     7741 gacgctcgcc accaccaatc gcgttgtcat cgccggcgaa gtgcgcacct cgctcgatga
     7801 gaagaccatg aagcgcaagg tcaagtccgc cgcgcgcaag gcgatcaagg cgatcggcta
     7861 cgagcaggac ggcttccact ggaagaaggc caagatcgac gttttgctgc attcccagtc
     7921 cgctgacatt gcgcagggcg ttgattccgc cggcaacaag gatgagggcg ccggcgacca
     7981 gggcatcatg ttcggttatg cctgccgcga gaccaagggc ctgatgccgg cgccgatcta
     8041 ctacgcgcac aagattctgg aagacctgac ggcgctgcgc aagaagggcg agcgcaattg
     8101 cggcaagctc ggccccgacg ccaagagcca ggtcacggtt cgctacgaga acggcaagcc
     8161 cgtcgccgtg acccagatcg tgctgtccac ccagcacctt gacgagaagc tgacctccaa
     8221 ggacgtgcag aagatcgtcg agccgatcat ccgcgagacc ctcgcggaca tcgagatcgc
     8281 caaggactgc aagtggtggg tgaaccccac cggcaagttc gtcatcggcg gcccggatgg
     8341 cgattgcggc ctcaccggcc gcaagatcat cgtcgacacc tacggcggcg cggcgccgca
     8401 cggcggcggc gccttctcgg gcaaggaccc gaccaaggtg gatcgctccg ccgcctatgc
     8461 cgcacgctac ctcgccaaga acgtcgttgc ggcgaagctt gccgaccggt gcaccatcca
     8521 gctctcctac gccatcggcg tggccaagcc gctgtcgatc tatgtcgacc tgcacggcac
     8581 cggcaaggtg gacgaggcaa aactggagaa ggctctgggc gaggtcatgg acctcacccc
     8641 gcgcggcatc cgcacgcacc tcggcctcaa caagccgatc tacgcccgca ccgccgccta
     8701 cggccatttc ggccgcaagg cgtcgcgcga cggcggcttc tcctgggaga agacggacct
     8761 cgtcgatgcg ctgaagcggg ctgtcgcctg acgcagcatg gcggatcacg acaccgcgac
     8821 tgaccgtgcc gccggcttct ttggccggcg caagggacac aggctgcggg atgagcaggc
     8881 ggtgcgtctg gaccgcctgt tgccggccct gcggatcgac atggccaggc cttttggcga
     8941 tcccaagacg ctgttccccg aaaacgtcga aggcgtgcat ctcgaaatcg gctttggcgg
     9001 gggagaacac ctggtcgatg ccgcgtcccg gcatcccggg atcggcttca tcggctgcga
     9061 gccgtttgtg aatggcgtcg ccaaactggt ggcggaaatc gagaaacgcg gcctgaccaa
     9121 catccgcgtg catgacgcca acgccgccga cctcctgccg ctgttgccgc ctgccagcat
     9181 cgagcgggtc tatctgctct atcccgaccc ctggccgaag cggcgccagc gcaagcggcg
     9241 ctttgtctcg cctgccgctg tgaaagccct ggcgcgcgtg ctgaagccgg gtgggacctt
     9301 tcgcttcgcg accgatattg acgactatgc cggatggacg ctggcgcgca tggccgccag
     9361 ccccgacttc gtctggactg ccgagcgggc tgatgactgg cgcgcgccct acgccaactg
     9421 gccgggaacg cgctacgagg cgaaggccaa gcgcgaaggg cgcacgccca gctatctgga
     9481 attccggcgg gtctgatccg gttcaggacg cgccacgttt ggcgagcatc agtgccctta
     9541 actcttttcc ggcgtcctct gggtcgtcgg caagaccggc aacaacgccg tcgcgatcct
     9601 cggtgctgcg attctggctc agcttctgtt tgaaggccag cgtatcgatc accagctcga
     9661 cgccgacgat tccgcgcagc atcgcagcga tatagctctc cggcgcgtcg gacacctgcc
     9721 atggcgcggg gcgctgcgct tcgaacctgt cggtcagcac cgtcacgatt cggcgcaggt
     9781 ccgccgcgtc atcgatccac cgcagcattc cgcgcgcctg cacgacgaca taattccagg
     9841 tcggaacggc ctttccttcc accttcttgg aaggatacca gtttggcgag acataggcgt
     9901 ccggaccgga aaagatcgcc agcgatggcc ggcccggcaa ggccagtttc cagtgcgggt
     9961 tggcgcgggc gacatggccg atcaggcgac cgttcggccc gcccgccggc tcgaacagca
    10021 tcggcagatg cgaggcaaac gggccgtc
//
LOCUS       DAOMXN010000077         9996 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_3569_length_9996_cov_10.465849, whole genome shotgun sequence.
ACCESSION   DAOMXN010000077 DAOMXN010000000
VERSION     DAOMXN010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 9996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9996
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3569_length_9996_cov_10.465849"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="PK812_07205"
     CDS             complement(<1..835)
                     /locus_tag="PK812_07205"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HRJ69380.1"
                     /translation="MAKAANSNTEPVHDVAVVGGGAAGLAVLLGLERAGLKPVLVGAV
                     PNPRDDGRTAALMAPSVRLLDRIGALPRLEPKSTPLSAMRLIDATGALVRAPTVTFRA
                     SEVGLPWFALNFANADLVREMTAEAAHRGLDCIAEQVIEGAFGEDCARLVLADGQILR
                     ARLVIAADGQKSLLRGLAGLNIKSWTYAQTALTFHVTHSRDHEDISTEIHMRAGPLTF
                     VPFGAYRSAVVWLVTPDEAERLRALPPEDLAIAVQRASTSLLGDLTVVTPAAAVPMRG
                     LV"
     gene            915..1715
                     /locus_tag="PK812_07210"
     CDS             915..1715
                     /locus_tag="PK812_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HRJ69381.1"
                     /translation="MFDWIFSPEALAALAALTALEILLGIDNVIFISVLVSRLPREQG
                     EIVRKLGLLLAFLIRIILLFAIATLLKLSQPVLTLFGSVFSWRDIILVAGGLFLIVKA
                     THEMHLELEGGDHLVADGRKPRAFGAAVAQVAVIDLVFSVDSIVTAIGMTRILPVMVT
                     AVAISMLVMWLAAGPVAAWLIRHPTTKMLALAFLLLIGVVLFMEGFGQVIDRRLIYVA
                     MAFALAIELINLRAGSNRQRPRRKRRSVPPPAVLQSGPEARNSDEARS"
     gene            complement(1670..3358)
                     /locus_tag="PK812_07215"
     CDS             complement(1670..3358)
                     /locus_tag="PK812_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HRJ69382.1"
                     /translation="MTQSRAAAVVEVFQPKLFTALKQGYGLDRLRADATAGLTVAIVA
                     LPLSMAIAIASGATPERGLYAAVIGGFIISALGGSMHQIGGPAGAFIVLVASSIERHG
                     LDGFFLATIIAGFIMIALGAFRLGTLIRYIPEPVTVGFTAGIAVIIAASQLRDLFGLT
                     LEGKEPAALIPKLGVLFAAFPSFNLYAIGVALFSGALILGIQRINKALPSLLIAVIAA
                     SLLVWAFNLPVETIGSRFGGVPSGLPAPALPTLSFAKVQAVLPDALAIALLGAIESLL
                     SAVVADSMTGRRHRSNTELVAQGFANLACPLFGGLCATGTIARTATNIRSGATSPVAG
                     MLHALFILAAMVLAAPLMARIPLAALGALLAIVAWNMAEKHTFWALARGNRAEAAVLL
                     ATFLLTVFRDLTEGILVGVVLGSVLFAVRMAQLAEVSSQMRLFDADGEDSAPHGARGS
                     GDIIIHRLDGPLFFGTAPNMQEVMGRIGHTPKAMVFDFSGVPFMDSSGVAMLDRHIRK
                     VEQTGAKVYFSGVSAPVELALRQFGLPKSRVPRLKNLQEAYDRASSELRASGPL"
     gene            3508..4482
                     /locus_tag="PK812_07220"
     CDS             3508..4482
                     /locus_tag="PK812_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HRJ69383.1"
                     /translation="MTKAIRVHECGGPECMVLEDVVLPAPGKGEVRVKHRAIGLNFID
                     VYFRSGLYKAASMPFTPGNEGAGEVVAVGEGVSGFRKGDRVAYVAPPGSYAEERNVPA
                     HFLVKLPKDIKYETAAGMMLKGMTAEYLLFRVHKVKKGDVVLVHAAAGGVGLILCQWA
                     NALGATVIGTVGSAEKAKLAKKNGCHHTILYRDEDFVARAKEITKGALCHVVYDGVGK
                     ATFPGSLDCLRPLGMFASFGSASGQIDAFNIGILGPKGSLFVTRPTLFTYIADRKRLV
                     QMAGRLFKAVQAKQVKIPVTAKFRLADVAEAHRALEGRATTGSVVLIP"
     gene            4529..6058
                     /locus_tag="PK812_07225"
     CDS             4529..6058
                     /locus_tag="PK812_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ69384.1"
                     /translation="MTETPLVHCTGLTKRFGSLVANDSVEVRVEQGEVCALLGENGAG
                     KSTFVKMLYGLLRPDAGRIEWLGREVTLESPEEARRLGIGMVFQHFSLFEEMTVLENI
                     ALGTDGARADRALADKVTKVAETYGLQLDLKRPVWTLSAGERQRIEIARCLLQEPRLI
                     VLDEPTSVLTPQESENLFKSLERLRDQGAGILFISHKLEEVRRLCTRATILRGGKLVG
                     VADPRKETARSLAAMMVGEGFAEVRPAPEHAHGPAILSVSGLSVDPDIVHGMPLKDVA
                     LDVHAGEVVAVAGVAGNGQSELFAALSGEGGIVTGGTVHISGEDVTWAAIGRRRKLGA
                     AYVPEGRLGHATIPQRTLSENIALSWHETEDIGGRVLSLAKLKAAAAEVIKRFDVRGP
                     NADPRANQLSGGNLQKYIVGREIQRNPRLIVVDQPTWGVDARAAALIRQTLVDLAAKG
                     AAVLAITQDLDEAFEISDRIAVIAEGRLHAPVATRATSREAVGLLMTAGHGDAHHGEA
                     A"
     gene            6207..7262
                     /locus_tag="PK812_07230"
     CDS             6207..7262
                     /locus_tag="PK812_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ69385.1"
                     /translation="MLTLVPRTGVSRRREIVTPLAAFAVALAVFAVVLLFMGRSPVKA
                     FQVYILESLTSSWSLQEVILKATPLTLIAIGLLFAVRANRWNIGAEGQYVVGGIFGGA
                     VAIWSHGNGGFWILPAMLIAGTIGGMLYGLIPAMLRNRFGISEILSSLMLVYVAELML
                     DYMVRGPWRDPRAFNFPKSVNFDAAATLGPIIEGGKLHWGVTFALIAVIAAYFLLGRS
                     LFGFAVSATGEAPKAANFAGFNEKWLTLAVFAISGGLAGLAGIIEVSAQIGQLQPSIS
                     QGYGFTAIIIAFLGRLTPVGALLSALVLAMLLIGSEAAQITLKLPFDMTRAFMGLLLL
                     CVLAGEALTRYRLEWRR"
     gene            7273..8190
                     /locus_tag="PK812_07235"
     CDS             7273..8190
                     /locus_tag="PK812_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ69386.1"
                     /translation="MELTTLILLTIITAATPLIIAGLGEHVVERAGVLNLGVEGMMIM
                     GASVGFAGAVIFDNSYVGLLCGTLAGAVMAGVFAAFALGLATNQVATGLAVTIFGLGL
                     AGLLGAPYVGQPRPVVAPIAIPLLSDLPVIGRALFKQDIVVYASFALIAGTAYVLNST
                     RVGLILRGVGESHGSSHALGLPVLKVRLLAVLFGGLCAGLAGAYLSLIYTRFWSPGMT
                     AGRGWIALALVVFAAGHVWRLAAGAYLFGAILVAQLHAQAGAIGVPAQFLSMLPYLMT
                     IIALVVLSISGRRGAGAAGSLGQPFVPDR"
     gene            8230..9291
                     /locus_tag="PK812_07240"
     CDS             8230..9291
                     /locus_tag="PK812_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HRJ69387.1"
                     /translation="MLKYLMGAVAAVALAATPALAQQKLKIGFIYVGPVGDFGWSYQH
                     DQGRIAIEKAFGDKVETSYIENVPEADSARSIEQLARTGHKLIFTTSFGYMEPTLQVA
                     KKYPDVKFEHATGFKRDKNVSTYSAKFHEGRYIVGQIAGKMTKSNTIGYVGAFPIPEV
                     VAGINAFYLGAKSVNPNVKIKIVWANSWFDPGKEADAAKALLDQGVDVITQHTDSPAP
                     LQQAAARGKLAIGQASDMSKFAPNNILTSIIDDWSPYYIKRTKAVLDGTWTSTDTFEG
                     LSSGKVKMGPYVNMPADVKAMAEATEKAISSGALNPFKCPILNQEGKEVAECKGALTD
                     AQINSMNYYVQGIDDKLPK"
     gene            9432..>9996
                     /locus_tag="PK812_07245"
     CDS             9432..>9996
                     /locus_tag="PK812_07245"
                     /inference="COORDINATES: protein motif:HMM:NF015863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HRJ69388.1"
                     /translation="MLKIWYLRLHGWIALIFSLPLLALILTGLILSVEPIMQVSTVKP
                     GSITTEKVLGWLAEHDAEGKARSLSHRPYDHLFSIEGAGPEGEVEIDLRTGKEFADGV
                     DAWYWSDWITWARRTHEHLTVYNVELTVVSTYVMILVILIGILMGLPKIRNNVAGWHK
                     ATAWFLLPLVIASPLTGLFITHRISLNF"
BASE COUNT         1700 a         3138 c         3400 g         1758 t
ORIGIN      
        1 ccacgagacc gcgcatcggc accgccgccg ccggcgtcac caccgtgagg tcgccaagca
       61 ggctggtgct ggcgcgctgg acggcgatgg ccaggtcttc tggcggtagc gcgcgcagcc
      121 gctcggcctc gtccggggta accagccaga ccacggccga gcggtaagcg ccaaaaggca
      181 cgaaggtcag cggacccgcc cgcatgtgaa tctctgtcga gatgtcttca tgatcgcggc
      241 tgtgcgtcac atgaaaggtc agcgcggtct gggcgtaggt ccagctcttg atgttgaggc
      301 ccgccaggcc gcgcagcagc gatttctgcc catccgcggc gatgactagc cgggcacgaa
      361 ggatctggcc gtcagccaga acgagccggg cgcagtcctc gccgaatgcg ccttcgatga
      421 cctgttcggc gatgcagtcg aggcctctgt gcgcggcttc cgccgtcatt tccctgacaa
      481 ggtccgcgtt ggcgaagttc agggcgaacc acggcagccc aacctcgctg gcgcgaaagg
      541 tcacggtcgg cgcgcgcacc agcgcacccg ttgcgtcgat caggcgcatg gccgagagcg
      601 gcgtgctctt cggttccaga cggggcaggg cgccgatcct gtcgaggagg cggacactcg
      661 gcgccatgag cgcagcagtc cgcccgtcgt cgcgcgggtt cggaacggcg ccgacgagaa
      721 ccggtttcag cccggcacgt tccagcccga gcagaacggc aagtccggcc gccccaccgc
      781 cgacaacggc gacgtcgtga accggctcgg tattcgaatt tgcagccttg gccatcgcag
      841 actcttgatt gacgggtgca aagtgtggcc tagctctgtc gggatcaagt gggcgctttg
      901 ccgcgagggg acggatgttc gactggattt tttctcccga ggctctggcg gcgctggctg
      961 cgctgacggc gctggagatc ctgctcggca tcgacaatgt catcttcata tccgttctcg
     1021 tctcgcggct gccgcgcgag cagggcgaaa tcgttcgcaa actcggactt ctgctggcct
     1081 tcctgatccg gatcatcctg ctgttcgcca tcgcgacgct gctcaagctg tcacagcccg
     1141 tgctgacgct cttcggttcg gtgttctcct ggcgcgacat catcctggtg gcgggcggcc
     1201 tgttcctgat cgtcaaggcg acgcatgaga tgcaccttga gctggaaggc ggtgaccacc
     1261 ttgtcgcgga tggccgtaag ccccgggcct ttggtgcggc cgtggcgcag gtggcggtga
     1321 tcgacttggt cttttccgtc gattccatcg ttaccgcgat cggcatgacg cgcattctgc
     1381 cggtgatggt cacggcggtc gccatttcca tgctcgtgat gtggctggcc gctggtccgg
     1441 tcgccgcctg gctgatccgc catcccacca cgaaaatgct ggccctcgcc ttccttttgt
     1501 tgatcggcgt cgtgctgttc atggaaggct ttggccaggt catcgaccgc cggctgatct
     1561 atgtggcgat ggccttcgcc ctggcaatcg aattgatcaa cctgcgcgca ggatcgaacc
     1621 ggcaacgtcc gcggcgcaaa cgcaggtccg tgccgccgcc cgctgtgctt cagagcgggc
     1681 cggaggcgcg caattccgac gaggcgcggt cgtaggcttc ctgcaggttc ttgaggcgtg
     1741 gaacgcgcga cttcggcagg ccgaactgtc tgagcgccag ttcgacgggg gccgaaacgc
     1801 cggaaaaata cactttcgcg cccgtctgtt ccaccttgcg gatgtggcga tccagcattg
     1861 ctacgccgga cgaatccatg aagggcacgc ctgaaaagtc gaacaccatc gctttgggcg
     1921 tatggccgat gcgccccatg acctcctgca tgttcggcgc cgtgccgaag aaaagggggc
     1981 catccagccg gtgaatgatg atgtcaccgg aaccgcgggc accatgcggc gcgctgtcct
     2041 cgccatcggc gtcaaacagg cgcatctggg atgagacctc cgccagttgc gccatgcgca
     2101 ccgcgaaaag gaccgagccg agcaccacgc cgacaagaat gccttcggtc agatcacgga
     2161 acacggtgag gagaaacgtt gccagcagca ccgccgcttc tgcgcgattt ccgcgtgcga
     2221 gcgcccagaa cgtgtgcttt tcggccatgt tccaggccac aatggccagc agcgcgccca
     2281 gtgcggccaa cggaatgcgg gccatcagcg gcgcggccag caccatcgcc gccaggatga
     2341 acagggcatg cagcataccg gccacggggc tggtcgcgcc ggagcggatg ttcgtcgctg
     2401 tacgggcgat ggtcccggtg gcgcagagcc cgccgaacag cgggcaggcc agattggcga
     2461 agccctgcgc caccagttcg gtgttcgagc gatggcggcg gccggtcatg ctgtcagcga
     2521 cgacggccga gagcagggat tcgatggcgc cgagcagggc gatggccagg gcgtccggca
     2581 gcacggcctg gaccttcgcg aaactcagcg tgggcagcgc gggagctggc aagccggagg
     2641 gcacgccacc gaagcgcgaa ccgatggttt cgaccggcag gttgaaggcc cagaccagca
     2701 gtgaagccgc gatgacggcg atgagcaggc ttggcaatgc cttgttgatc cgctggattc
     2761 cgaggatcag cgcgcctgaa aacaatgcga cgccaatggc atagaggttg aagctcggaa
     2821 atgcggcgaa cagcacgccg agcttgggga tcagcgccgc cggctccttt ccttcgagcg
     2881 tcaggccaaa caggtcgcgc agctggcttg cggcgatgat cacggcgatg ccggcggtaa
     2941 agccgaccgt caccggctcc gggatgtagc ggatcagggt cccgagccgg aatgcgccga
     3001 gcgcgatcat gatgaagccg gcgatgatgg tggccaaaaa gaagccatca agcccgtgcc
     3061 gctcgatcga cgaggccacc aggacgatga acgcgccggc ggggccgccg atctggtgca
     3121 tcgagccgcc gagggcagaa atgatgaatc cgccgatcac ggccgcataa agcccgcgtt
     3181 ccggcgtcgc gccggaggcg atggcgatcg ccatcgacaa gggcagggcg acaatggcga
     3241 cggtcaggcc ggccgtcgcg tcggcgcgca aacggtcgag gccgtatcct tgcttcagcg
     3301 cggtgaagag cttcggctgg aagacctcaa cgacggcggc cgcgcggctc tgggtcatgg
     3361 caggcgcttt caatcgactc ggacggaaag gccatgttcg cgcgctttgg ccgattgcgc
     3421 aaaataatgc gatggagaac cgcaaaaaac ctctggtcag aacggtcaag ccgcataaag
     3481 tgcctgagat agccgggagg ggcgacgatg acgaaggcaa tccgcgttca tgaatgcggc
     3541 ggtcccgagt gcatggtgct ggaggatgtc gtccttccgg cgccgggcaa gggcgaagtg
     3601 cgtgtgaagc accgtgctat cggcttgaat tttattgatg tttactttcg ctccggcctc
     3661 tacaaggccg cctcgatgcc gttcacgccg ggcaacgagg gtgcgggcga ggtggtcgcg
     3721 gtgggcgagg gcgtcagcgg gttccgcaag ggtgaccgcg ttgcctatgt ggccccgccc
     3781 ggatcctatg ccgaggagcg gaacgttccc gcgcatttcc tcgtcaaatt gcccaaggat
     3841 atcaaatacg aaaccgctgc cgggatgatg ctgaagggca tgacggcgga gtatctcctg
     3901 tttcgcgtcc acaaggtgaa gaagggcgac gtcgtgctcg tccatgccgc agcgggcggc
     3961 gtcggcctca tcctctgcca gtgggcgaat gcgctgggcg ccacggtgat cggcacggtg
     4021 ggatcggcgg aaaaagcaaa acttgccaag aagaatggct gtcaccacac gattctctac
     4081 cgggatgaag atttcgtcgc ccgcgcgaaa gaaatcacca agggtgcgct gtgccatgtc
     4141 gtctatgacg gcgtcggcaa ggcgaccttc ccgggctccc tggactgcct gaggccgctc
     4201 ggcatgttcg cgagtttcgg gtcggcttcg ggacagatcg atgcgttcaa catcggcatt
     4261 ctagggccga aaggctcgct tttcgtgacc cggccgacgc ttttcaccta catcgccgac
     4321 cgcaagcggc tggtgcagat ggccgggcgc ctgttcaagg cggtgcaggc caagcaggtg
     4381 aaaatccccg tgacggcgaa gttcaggctg gccgatgtcg ccgaggcgca tcgcgcgctg
     4441 gaagggcggg cgaccaccgg ttccgtcgtc ctgatcccgt agaaatgctc gtagaaccga
     4501 aaatgctcgg agcggaacag ggccgttcat gaccgaaacg ccgctggttc actgcacggg
     4561 acttacaaag cggttcggat cgctggtcgc caacgattct gtcgaggtcc gggtggagca
     4621 gggcgaggtt tgcgcgctgc tcggggagaa cggtgcgggc aagtcgacct tcgtgaagat
     4681 gctctacggg ctgttgcggc ccgacgccgg ccgcatcgag tggctcggtc gcgaggtgac
     4741 gctggaaagc ccggaggagg cgcgccggct cggcatcggc atggtgttcc agcacttctc
     4801 gctgttcgag gagatgacgg ttctggagaa catcgcgctc ggcaccgatg gggcgcgggc
     4861 cgaccgtgcg ctggcggaca aggtgacgaa ggtcgccgag acctacgggc tccagctcga
     4921 tctcaagcgg ccggtctgga cgctgtccgc aggcgagcgc cagcgcatcg agatcgcccg
     4981 ctgcctgctg caggaaccca ggctgatcgt gctcgacgaa ccgacctcgg tgctgacgcc
     5041 gcaggagtcg gaaaacctgt tcaagagcct cgaacgtctg cgggaccagg gggccggtat
     5101 cctgttcatc agccacaagc tggaagaggt gcgccggctg tgcacgcggg cgacgatcct
     5161 gcgcggcggc aagctggtgg gcgtcgccga tccgcgcaag gaaacggcgc ggtcgctggc
     5221 cgcgatgatg gtgggcgaag ggttcgcgga ggtgcgtccc gcacctgagc acgcgcatgg
     5281 tcctgccatc ctgtcggtgt ccggcctttc ggtggacccg gacatcgtgc acggcatgcc
     5341 gctgaaggac gttgcgctcg acgtgcatgc cggcgaggtg gtcgccgtgg ccggcgtcgc
     5401 gggcaacggc cagagcgaac tcttcgccgc gctgtccggc gaaggcggca tcgtcacggg
     5461 cggaacggtg cacatcagcg gcgaggatgt gacttgggcc gccatcgggc gccgccgcaa
     5521 gctcggcgcg gcctatgtgc cggaaggccg cctcggccac gcaacgattc cccagcgcac
     5581 gctgagcgag aacatcgcgc tgtcctggca cgagaccgag gacatcggcg ggcgggtgct
     5641 gtcgctcgcc aagctgaagg ccgccgccgc cgaggtgatc aagcggtttg acgtgcgcgg
     5701 gccgaatgcc gatccgcgcg ccaaccagct ctccggcggc aatttgcaga aatacatcgt
     5761 cggtcgcgag atccagcgca atcccaggct catcgtggtt gatcagccca cctggggcgt
     5821 tgatgcccgc gctgcggcgc tgatccgcca gacgctggtc gatctcgcgg ccaagggcgc
     5881 ggcggtgctg gcgatcacgc aggatctgga cgaggccttc gagatttccg accgcatcgc
     5941 ggtgatcgcc gaagggcggc tgcatgcgcc ggtggcgacg cgcgcgactt cgcgcgaggc
     6001 cgtgggcctg ctgatgaccg ccggccatgg cgacgcgcat cacggggagg ccgcgtgatg
     6061 gcgagccgga ccgttttccc gaccgtacgg gttgcagacg atcccttcgc cgcgtggggc
     6121 ggggcagggg tcatcacgag gcccctcacc ccgaccctct ccccgaacgc ggggagaggg
     6181 agagttgaac cgttcggggc tctcccatgc tgacgctcgt tccccgcacc ggcgtatcgc
     6241 ggcggcgcga gatcgtcacg cctcttgcgg ccttcgcggt cgcgctggcg gtgttcgcgg
     6301 tcgttctgct gttcatgggg cggtccccag tcaaggcctt ccaggtctac atcctggaat
     6361 cgctgacgag ttcctggtcg ttgcaggagg tgatcctcaa ggcgacgccg ctgacgctga
     6421 tcgccatcgg cctgctgttc gcggtccggg ccaaccgctg gaatatcggc gcggaagggc
     6481 agtatgtcgt cggcggcatc ttcggcgggg cagtagcgat ctggagccac ggcaacggcg
     6541 gcttctggat cctgccggcg atgctgatcg ccggcacgat cggcggcatg ctctacgggt
     6601 tgatcccggc gatgctgcgc aatcgcttcg gcatcagcga aatcctgtcc agcctgatgc
     6661 tggtctatgt ggcggagctg atgctcgact acatggtgcg cggaccgtgg cgcgacccgc
     6721 gcgccttcaa cttcccgaaa tcggtgaatt tcgacgcggc cgcgacgctg gggccgatca
     6781 tcgagggcgg caagctgcac tggggcgtca ccttcgcgct gatcgcggtg atcgcggcct
     6841 atttcctgct cgggcgctcg ctgttcggct tcgccgtcag cgccacgggc gaggccccga
     6901 aggccgccaa tttcgccggt ttcaacgaga aatggctgac cctggcggtt ttcgccattt
     6961 ccggcgggct ggcggggctt gccgggatca tcgaggtatc ggcgcagatc ggccagttgc
     7021 agccctcgat cagccagggc tacggcttca cggcgatcat catcgccttc ctcgggcggc
     7081 tgacgccggt gggcgcgctc ctctccgcgc tggtgctggc gatgctgctg atcggctccg
     7141 aggcggcgca gatcacgctg aagctgccgt tcgacatgac ccgcgccttc atggggctgc
     7201 tgctgctctg cgtgctcgcg ggcgaggcac tgacccgcta ccggctggag tggcggcgat
     7261 gaggactgtc ccatggaact gaccaccctc attctcctca ccatcatcac ggcggcgacg
     7321 ccgctgatca tcgccggcct cggcgagcat gtggtggagc gcgccggcgt gctgaacctc
     7381 ggcgtcgagg gcatgatgat catgggcgcc tcggtcggtt tcgccggggc ggtgatcttc
     7441 gacaattcct atgtcggcct cctgtgcggc acgctggccg gggcggtgat ggcgggcgtg
     7501 ttcgcggcct tcgcgctggg acttgccacg aaccaggtgg cgaccggcct tgccgtgacg
     7561 atcttcggcc ttggcctcgc cggcttgctg ggcgcaccgt atgtcggcca gccccgacct
     7621 gtggtggcgc ccatcgccat ccccttgctg agcgatctgc cggtgatcgg ccgcgcgctg
     7681 ttcaagcagg acatcgtcgt ctacgcctcc ttcgcgctga ttgccggcac ggcctacgtg
     7741 ctgaactcga cacgggtggg gctgatcctg cgcggcgtcg gcgagagcca cggctcctcc
     7801 cacgcgctcg gcctgccggt gctgaaggtg cggctgctgg cggtgctgtt cggcgggctc
     7861 tgtgcgggcc ttgcgggcgc atacctgtcg ctgatctaca cgcggttctg gtcgcccggc
     7921 atgacggcgg gacgcggctg gatcgcgctg gcgctggtgg tgttcgcggc ggggcatgtc
     7981 tggcggcttg ccgccggggc ctatctgttt ggcgcgattc ttgtagcgca gctgcatgcg
     8041 caggccgggg cgatcggcgt tccggcgcag ttcctgtcga tgctgcccta cctgatgacg
     8101 atcatcgcgt tagttgtctt gtcgatatca ggacggcgcg gggcaggagc cgcaggctcg
     8161 ctcggtcagc ccttcgtgcc ggatcgatga aaccgcattg agaacccaac cacaaaggag
     8221 aggcacacca tgctgaaata cctgatgggc gcagtcgccg cggtggcgct ggccgcaaca
     8281 ccggcgctgg cacagcagaa gctgaagatc ggcttcatct acgtcggccc ggtgggcgac
     8341 ttcggctggt cctaccagca cgatcagggc cgcatcgcca tcgagaaggc gttcggcgac
     8401 aaggtcgaga cgtcgtacat cgagaacgtg ccggaggccg acagcgcccg ctcgatcgag
     8461 cagctggccc gcaccggcca caagctgatc ttcaccacct cgttcggcta catggaaccg
     8521 acgctgcagg tggcgaagaa gtatccggac gtgaagttcg agcacgccac cggcttcaag
     8581 cgcgacaaga acgtctcgac ctattcggcc aagttccacg agggccgcta tatcgttggg
     8641 cagatcgccg gaaagatgac caagtcgaac accatcggct atgtcggcgc cttcccgatc
     8701 ccggaagtgg tggccggcat taacgccttc tatctcggcg ccaagtcggt gaacccgaac
     8761 gtcaagatca agattgtctg ggcgaactcg tggttcgatc cgggcaagga agcggacgcc
     8821 gccaaggcgc tgctcgacca gggcgtcgac gtgatcaccc agcacaccga cagcccggcg
     8881 ccgctgcagc aggccgccgc gcgcggcaag ctcgccatcg gccaggcctc ggacatgtcc
     8941 aagttcgcgc cgaacaacat cctgacctcg atcatcgacg actggtcgcc ctattacatc
     9001 aagcgcacca aggccgtgct cgacggcacc tggacctcga ccgacacgtt cgagggcctt
     9061 tccagcggca aggtgaagat gggcccctac gtcaacatgc cggctgacgt gaaggccatg
     9121 gcggaagcca cggaaaaggc gatctcttcc ggagcgctca atccgttcaa gtgcccgatc
     9181 ctcaaccagg agggcaagga agtggccgag tgcaagggcg cgctgaccga tgcccagatc
     9241 aattcgatga actattacgt gcagggcatc gacgacaagc tgccgaaata aggcagtccg
     9301 gatcgcgacg tgacgacgcg gcggggcggc gatttgccgc cccgcttgca tgaatgatta
     9361 caaaggattc atttgaaccc gtgtttcagg gttctatggt gcgccgcacc ttcctgcttc
     9421 gaggcggccc catgctcaaa atatggtatc tgcggctcca tggctggatc gcgctgattt
     9481 tctcgctgcc gctgctggcg ctgatcctca ccggactgat cctctccgtc gagccgatca
     9541 tgcaggtttc cacggtgaag ccgggctcga tcaccaccga gaaggtgctg ggctggctcg
     9601 ccgagcacga cgccgagggc aaggcgcggt cgttgtcgca tcgtccctat gatcacctgt
     9661 tctcgatcga aggcgcgggg ccggagggcg aggtcgagat cgacctgcgc accggtaagg
     9721 agttcgccga tggcgtggac gcctggtact ggtccgactg gatcacctgg gcgcggcgca
     9781 cgcacgagca cctgaccgtc tataatgtcg agctgacggt cgtttcgacc tacgtcatga
     9841 ttctggtcat cctgatcggc atcctgatgg ggctgccgaa aatccgcaac aatgtcgcgg
     9901 gctggcacaa ggcgacggcc tggttcctgc tgccgctggt catcgcaagc ccgctgacgg
     9961 gattgttcat cacgcatcgc atctcgctga actttt
//
LOCUS       DAOMXN010000078         9988 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_3578_length_9988_cov_12.510218, whole genome shotgun sequence.
ACCESSION   DAOMXN010000078 DAOMXN010000000
VERSION     DAOMXN010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 9988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9988
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3578_length_9988_cov_12.510218"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="PK812_07250"
     CDS             <1..732
                     /locus_tag="PK812_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003599296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HRJ69389.1"
                     /translation="IGAARPASGFHYFDADTPLSPDSYDVALHALGSAVRAVDAVMTG
                     EVANAFVAARPPGHHAEKDTAMGFCFFNTVAVAARHAQKAFGAERIAIVDWDVHHGNG
                     TQDIFWADRSVLFCSTHEMPLYPGTGAPGERGEHNTILNAPLRAGDDGHAFSEIFRDL
                     VLPRVRNHAPDLILISAGFDAHKRDPLANIQLDESDFAWATEELMEIAATRCKGRIVS
                     LLEGGYDLQGLATSVANHVHRLMGA"
     gene            complement(763..1851)
                     /gene="aroC"
                     /locus_tag="PK812_07255"
     CDS             complement(763..1851)
                     /gene="aroC"
                     /locus_tag="PK812_07255"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HRJ69390.1"
                     /translation="MSHNSFGHLFRVTTFGESHGPAIGCVVDGCPPRIPLSESDIQPW
                     LDRRRPGQSRFTTQRQEPDQVKILSGVYTEADGVQRTTGTPIGLLIDNVDQRSKDYTA
                     IGDQFRPGHADFTYDAKYGIRDPRGGGRSSARETAMRVAAGAIARRIVPGLKVYGALV
                     QMGPHRIDRSRWHTESIVENPFFCPDPQMVGVFETFLDGVRKSGSSTGAVIEIIAEGV
                     PAGLGAPVYGKLDSDLASALMSINAVKGVEIGAGFAAAGLSGEENADEMRIGNDGRPY
                     FCSNNAGGILGGISTGQAVVARFAVKPTSSILTTRRSIDRYGAEIDIMTKGRHDPCVG
                     IRAVPVGEAMVACVIADHYLRHRGQLGS"
     gene            complement(1908..2498)
                     /locus_tag="PK812_07260"
     CDS             complement(1908..2498)
                     /locus_tag="PK812_07260"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRJ69391.1"
                     /translation="MTGPAIYFIRHGETDWNREGRLQGGQEIPLNDLGRSQAAVAAEH
                     LRDLVGERTRTLPWLASPMGRTVETLQIMRRTLGLPAGGWASDERLREISFGRWEGLT
                     WKEVRRADPPGAAGRSADKWGYVPPGGESYQILQERVRPVFEAIVTDTIVVAHGGVAR
                     AALVLLAGMAPMAAVNEDIWQGRVLVIEPGRQFWVP"
     gene            complement(2527..3009)
                     /locus_tag="PK812_07265"
     CDS             complement(2527..3009)
                     /locus_tag="PK812_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69392.1"
                     /translation="MRRIVLLLAVSVPALALAQGQPPLQPGFQTPPSAQPASTPAPAD
                     PVPEITTTEVQKQAKTGRDVFIGSYIQVDRTCKVGPTPKIEQPEPPKNGVIKTRPMPI
                     NLGSAPGVPRNKCLGVSPAGIAVYYQGKWRFKGEDAFVYRVRYADGRIREVKGTVTVQ
                     "
     gene            complement(3056..3874)
                     /gene="fabI"
                     /locus_tag="PK812_07270"
     CDS             complement(3056..3874)
                     /gene="fabI"
                     /locus_tag="PK812_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012322413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HRJ69393.1"
                     /translation="MAGGNGFMAGKRGLIMGVANNRSIAWGIAKAVHAQGAKLAFTYQ
                     GDALRKRVEPLAAELDAAVVGHCDVTDAASIDAVFAEVNKIWGGLDFVVHAIAFADKD
                     ELTGRYVETSEANFTKSMLISCYSFTAIAQRAEKLMANGGSLLTLTYYGAEKWMPHYN
                     VMGVAKAALEASVRYLAADLGPNNIRVNAISAGPIKTLAASGIGDFRYILKWNEYNSP
                     MRRTVTIDEVGDVGAYFCSDLSRGVTGEVHHVDCGYHIVGMKNPDAPDLSLDRD"
     gene            4096..4770
                     /locus_tag="PK812_07275"
     CDS             4096..4770
                     /locus_tag="PK812_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HRJ69394.1"
                     /translation="MKRSQINAAIRHAEALLQRHAFLLPPFAGWSPADWSANSGRTAE
                     IRRAGLGWDITDFGQGRFAELGLTLFTIRNGDARNLGTGKGMVYAEKAIIVREGQRCP
                     SHFHFVKTEDIINRGGGVLALELVETDASGQPDETREVVVRTDGLERRMPARSVLRLN
                     PGESVTLTPFLAHAFWAEGGDVFVGEVSTVNDDKTDNAFLEKVGRFPTIEEDEPSYRP
                     IVGDLG"
     gene            complement(4797..5585)
                     /locus_tag="PK812_07280"
     CDS             complement(4797..5585)
                     /locus_tag="PK812_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009762992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HRJ69395.1"
                     /translation="MCRWIAYRGRPVYLETVIVQPAHSLVHQSLHATESKTGTNGDGF
                     GVGWYGEKPDPGLYREVRPAWSDENLRSLCAQVRSPLFFAHVRASTGTATSRANCHPF
                     VHGQWMFMHNGQIGCFQHLRRRVEELIPDDLYTGRSGSTDSEAIFLAAMARMASHDPV
                     AAIASVLGDVKAMMQRCCVAEPLRFTAALTNGRDIYAFRWACDDKAPTLYWRIKPKGL
                     FIVSEPFDNERQNWNAMPQASVLVARAGEDVAVAPLDLGVRLAA"
     gene            complement(5661..6146)
                     /locus_tag="PK812_07285"
     CDS             complement(5661..6146)
                     /locus_tag="PK812_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69396.1"
                     /translation="MKHVLAAATLLALASAPVAAQQAGQGITVQDVKVQLFLERTGTL
                     SENLVGSKKALFNTPIGAGDAGEPAEAVLVTLVFQGPKNSRASDKLARDLAAIKVTQT
                     AKTGPKILMNRAFAGLLFGESGIVHKAVMLENATCAPLEIEARVGRSRKAVKIDFQCG
                     E"
     gene            6231..7637
                     /locus_tag="PK812_07290"
     CDS             6231..7637
                     /locus_tag="PK812_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HRJ69397.1"
                     /translation="MNRIYLLAAAVCIPGGPALAAGGLDGRALGIAWALPFFGLLACI
                     ALGPLFFARTWHHHYGKIAAVWALVTLLPLAALHGVGPAVEVLLHALLVEYVPFIILL
                     FALYTVAGGILISGTITGTPGTNTAILAFGTGLASVVGTTGASMILIRPLIRANDQRR
                     HNVHVVIFFIFLVSNIGGALTPLGDPPLFVGFLRGVDFFWTTRHLLAPMLLLSVLLLA
                     GFYLLDRWFWQRDAGLPPLKDFTPERSVKLFGKRNIPLLAAIIAVLLLTSAWKPGITV
                     PVPGAPMPLQGLVRDILLLVIAGLSLRLTPGPVRAGNEFSWEPIAEVGKLFAAIFVCV
                     APVIAMLQVGKEGVFAPLVAFVTSSGGQPIPQAYFWLTGLLSAFLDNAPTYLVFFELA
                     GGNAADLMTAKGQTLLAISCGAVFMGALTYIGNAPNLMVATIARDMKIQMPGFLGFMA
                     WSFGILVPLFLLVGWLFI"
     gene            7710..9122
                     /locus_tag="PK812_07295"
     CDS             7710..9122
                     /locus_tag="PK812_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HRJ69398.1"
                     /translation="MPLIARMAPLALTIISSPVMAAEGLDGKSFGIGWSLPFAGLLLT
                     MALAPLFLHRTWEHHYGKIAAGWALVVLIPLALTRGFGVTLEAFLHAMLVEYMPFIIL
                     LFALYTIAGGILVTGNLRGTPATNTTLLLIGTILASFVGTTGASMIMIRPVCRANDDR
                     RHNVHVIIFFIFLVSNVGGSLTPLGDPPLFVGFLRGVDFFWTTKALFWPTVFVAAVLL
                     AAFYALDTYLHAKDDKLPALKDKTPDRDIKIFGQVNFLLLAGVIAAILISASWKPGVS
                     LKLPGANLPVQGLVRDAVLLALAGLSLTLTPKQVRAGNEFSWGAILEVGKLFAAIFVC
                     VIPVLAMLQAGHQGALKGLVALVSNADGSPNPQMYYFLTGILSSFLDNAPTYLVFFEL
                     AGGDPQVLMTQKANILAAISLGAVYMGANTYIGNAPNLMVANIARDMGVKMPSFLGYM
                     VWSFAILMPVLVLVGFVLVK"
     gene            complement(9148..>9988)
                     /locus_tag="PK812_07300"
     CDS             complement(9148..>9988)
                     /locus_tag="PK812_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HRJ69399.1"
                     /translation="MLGLAPARLPNRTPTDGPGVHAPPGPRKGRVALLSGCAQSVLAP
                     GINDAAIRLVTRHGYEVIVPRNEGCCGALVHHMGREHESHEQARRMIDLWTAEIANGG
                     LDAILITASGCGTTIKDYGFMFRDDPAYADKAARVAALAMDITEFAAKLDLSVRPGPG
                     LTVAYHSACSMQHGQKITDLPKTLLRQAGFTVKDVPEGHICCGSAGTYNMLQPEISGS
                     LRARKVTNIERTGAPVIATGNLGCMTQIAKGFEERGETRAIVHTVELLDWATGGPKPE
                     GLG"
BASE COUNT         1578 a         3260 c         3227 g         1923 t
ORIGIN      
        1 atcggcgcgg cgcggccggc gagcggcttt cactatttcg acgccgatac gccgctttct
       61 cccgatagct acgatgtcgc gctccatgcc ctcggatctg ccgtgcgcgc ggtggatgcg
      121 gtgatgaccg gcgaggttgc caatgccttc gttgcggcgc ggccgcccgg ccatcatgcc
      181 gagaaggaca cggcgatggg gttttgtttt ttcaacaccg tcgcggttgc cgcccggcac
      241 gcccagaagg cctttggcgc ggaacgtatc gccatcgtcg actgggatgt gcatcacggc
      301 aatggcacgc aggacatttt ctgggcggac aggtcggtcc tgttctgctc cacgcacgaa
      361 atgccgctct accccggcac cggcgcgccg ggtgaacgcg gcgagcacaa caccattctg
      421 aacgctccgc tgcgggccgg cgacgatggt cacgcctttt ccgagatttt ccgcgatctg
      481 gtgctgccgc gcgtgcgcaa tcatgccccg gacctgatcc tgatttccgc cggcttcgat
      541 gcgcacaagc gcgatccgct cgccaacatc cagctcgacg agagcgattt tgcctgggcg
      601 accgaggaac tgatggaaat cgccgccacc cgctgcaagg gacggatcgt ctcgctgctc
      661 gaaggcggtt acgaccttca ggggctggcg acgagcgtgg ccaaccatgt gcaccggttg
      721 atgggggcgt gagatgcccg gtcgaccgaa ggtggattca gcctagctgc ccaactgccc
      781 gcgatgccgc aggtaatggt cggcgatgac gcaggcgacc atcgcctcgc ccaccggcac
      841 ggcgcggatg ccgacgcagg gatcgtggcg gcccttggtc atgatgtcga tctcggcgcc
      901 gtaacggtcg atcgaccggc gcgtcgtcag gatcgaggac gtcggcttga cggcgaagcg
      961 ggcgacgacg gcctgcccgg tcgagatgcc gccgaggatg ccgccggcgt tgttggagca
     1021 aaaatacggc cgcccgtcgt tgccgatacg catctcgtcg gcgttctcct cgccggagag
     1081 tccggcggcc gcgaacccgg cgccgatctc cacgcccttt acggcgttga tgctcatcag
     1141 cgcgctggcg aggtcggaat caagcttgcc gtagaccgga gcgccgaggc cggcagggac
     1201 gccctcggcg atgatctcga tcaccgcccc ggtggacgaa ccgctcttgc gaacgccgtc
     1261 gaggaacgtc tcgaatacgc cgaccatctg cggatcagga cagaagaatg gattttctac
     1321 gatgctttcg gtgtgccagc gcgaacggtc gatccggtgc ggacccatct gcaccagggc
     1381 gccatagacc ttcaggccgg gcacgatcct gcgggcgatg gcgccggccg ccacccgcat
     1441 cgcggtttcg cgcgccgagg aacggccgcc gccgcgcggg tcgcggatgc catatttcgc
     1501 gtcgtaggtg aaatcggcgt gacccgggcg gaactggtcg ccgatggcgg tataatcctt
     1561 cgagcgctgg tccacattgt cgatcaggag gccgattggc gtgcctgttg tgcgctggac
     1621 gccatcggct tccgtgtaaa ccccagacag gattttcacc tggtccggtt cctggcgctg
     1681 tgtggtgaag cggctctggc cggggcggcg ccggtcgagc cagggctgga tatcggattc
     1741 ggagagcgga atgcgcgggg gacagccgtc gacgacgcaa ccgatggccg gaccgtggct
     1801 ctcgccgaag gtcgtgacac ggaacaggtg accgaaggaa ttgtgagaca tgacagccgc
     1861 ttcgcgcggt tttcgagcgc cgtcaagcct tgtccgcgcc gacgacttca gggaacccag
     1921 aactgccggc cgggctcgat caccagcacg cggccctgcc agatgtcctc gttgactgcc
     1981 gccatgggcg ccattcccgc cagcagcacc agcgccgcgc gcgcgacgcc gccatgcgca
     2041 accacgatgg tgtccgtgac gatggcttca aacaccgggc ggacccgttc ctgcaagatc
     2101 tggtagcttt cgccgccggg gggaacatac ccccacttgt ctgcgctgcg tccggccgcg
     2161 cccggcggat cggcccggcg tacctctttc caggtgaggc cttcccagcg tccgaaggag
     2221 atttcgcgca gacgctcgtc gctggcccag ccgccggcgg gcaggcctag cgttcgccgc
     2281 atgatctgca gcgtttccac cgtacgcccc atggggcttg cgagccatgg cagcgttcgg
     2341 gtccgctcgc cgacaagatc gcgaaggtgt tccgccgcaa cagcagcctg gctgcgaccg
     2401 aggtcgttga ggggaatctc ctgcccgccc tgaagccggc cctcgcggtt ccagtcggtc
     2461 tcgccgtggc ggatgaaata gatcgcaggg ccggtcatgc cgggcccaac aaccggaggg
     2521 cgcgcgtcat tggaccgtga ccgtgccctt cacttcgcga atccgtccgt cagcgtagcg
     2581 gacacgatag acgaaggcgt cctcgccctt gaagcgccac ttgccctgat aatagacggc
     2641 gatcccggca ggcgagacgc cgaggcactt gttcctcggc acacccggcg cgctccccag
     2701 gttgatcggc atgggccggg tcttgatcac accgttcttt ggcggctccg gctgctcgat
     2761 cttgggcgtc ggcccgacct tgcaggtgcg atcaacctga atgtaggagc cgatgaacac
     2821 atcgcgcccg gtcttcgcct gcttctgcac ttcggtcgtg gtgatctccg gaaccggatc
     2881 ggccggtgcg ggtgtcgagg cgggctgggc cgacggcggg gtctggaatc caggttgaag
     2941 cggcggctgt ccctgcgcca gcgccagcgc cggaacgctc accgctaaaa gcaaaacaat
     3001 gcgccgcatc gatcaacctg cccgattttt tcggataatg ccctgattcg ccttcctagt
     3061 cgcggtccag agacagatcc ggggcgtccg ggttcttcat tccgacgatg tggtagccgc
     3121 aatcgacatg gtgaacctcg ccggtgacgc cgcgcgacag gtccgagcag aaataggctc
     3181 cgacatcgcc cacttcgtcg atggtcacgg tgcgccgcat cggcgaattg tactcgttcc
     3241 acttcaggat gtagcgaaaa tcgccgatgc cggaggcggc gagcgtcttg atcggcccgg
     3301 cggaaatggc attgacgcgg atgttgttcg ggccgaggtc tgcggcaaga tagcgcacgc
     3361 tggcttccag cgccgccttg gccacgccca tgacattgta atgcggcatc catttttcgg
     3421 cgccgtagta ggtcaacgtg agcagtgagc cgccattggc catcagcttc tcggcgcgct
     3481 gcgcgatcgc tgtgaaggaa tagcacgaaa tcagcatgga cttggtgaaa ttcgcctcgc
     3541 tggtctcgac atagcggccg gtcagctcgt ccttgtcggc gaaagcgatg gcgtggacca
     3601 cgaagtcgag cccgccccag attttgttca cctcggcgaa taccgcatcg atgcttgccg
     3661 cgtcggtcac atcgcaatgg ccgacgactg ccgcgtccag ttcggccgcg aggggttcca
     3721 cgcgtttgcg caacgcgtct ccctggtagg taaaggcgag tttcgccccc tgggcatgga
     3781 ccgccttggc gatgccccag gcaatcgagc ggttgttggc cacacccatg atgagcccgc
     3841 gcttgccggc catgaagccg ttgccgcctg ccatctctta ctccattcgc gctaagccga
     3901 gttgtggcat acctaaccga acacgcgccc tatgcaagcg cgaatgccgg tttcgggcga
     3961 tgcggcctga tcggagaacg ggcggcagcc cggattgccg tgccgcaaga aggcacttgc
     4021 cgagccgaag gcgggcagac atgactctgc cctgatacgc cgcatcgccc cttcgctcac
     4081 gctcaggccc gcaccatgaa acgctcccag atcaatgccg ccatccgtca tgccgaggcc
     4141 ttgcttcagc gccatgcctt cctgctgccg ccgtttgccg ggtggtcgcc ggcggactgg
     4201 tccgcgaaca gcgggcggac cgctgagatc cgccgcgccg gtcttgggtg ggacattacg
     4261 gacttcgggc agggccgctt tgccgaactc ggcctgaccc tgttcaccat ccgcaacggc
     4321 gatgcgcgca atctcggcac cggcaagggc atggtctatg ccgagaaggc catcatcgtg
     4381 cgtgagggcc agcgctgccc gtcgcacttt cacttcgtca agacggagga catcatcaac
     4441 cggggcggtg gcgtgctggc gcttgaactg gtggagaccg atgcttcggg acagcccgac
     4501 gaaacccgtg aggtcgtggt ccgcaccgac gggctggaac gccggatgcc cgcgcgcagc
     4561 gtcctgcgtc tcaaccccgg cgagagcgtg acgctgacgc cgtttctcgc ccatgccttc
     4621 tgggccgaag gcggcgatgt cttcgtcggc gaggtctcca ccgtaaacga tgacaagacc
     4681 gacaacgcct ttctggagaa agtcggtcgt ttccccacca tcgaggaaga cgaaccttcc
     4741 tatcggccca ttgttggcga cctggggtag actgtcaggc agccggaaag gccggatcag
     4801 gccgcgaggc ggacaccaag atcgaggggt gccaccgcca cgtcctcgcc ggcgcgtgcc
     4861 acgaggacgc tggcctgcgg catggcgttc cagttctgcc gctcgttgtc gaacggctcc
     4921 gagacgatga acaggccctt cggcttgatg cgccagtaca gggtcggcgc cttgtcgtcg
     4981 caggcccagc ggaaggcata gatgtcccgg ccgttggtga gggccgccgt gaaccgcagc
     5041 ggctcggcaa cgcagcacct ctgcatcatc gccttgacgt cgccgagcac cgaggcgatg
     5101 gccgcaaccg gatcatgcga tgccatgcgc gccatggcgg ccaggaagat ggcttccgaa
     5161 tcggtcgagc cgctgcgccc ggtatagagg tcgtccggga tcagttcctc cacgcgccga
     5221 cgcaggtgct ggaagcagcc gatctgaccg ttgtgcatga acatccactg gccatggaca
     5281 aagggatggc agttcgcccg gctggtggcc gtgcccgtcg aggcgcggac atgggcgaag
     5341 aacagcggcg aacggacctg cgcgcacagg gaacgcaggt tttcatcgga ccacgccggg
     5401 cgcacctcgc gatacaggcc cggatcgggc ttctcgccat accagccgac gccgaagccg
     5461 tccccgttcg tgccggtctt gctttctgtc gcgtgcaacg actggtgaac cagcgaatgc
     5521 gccggctgaa caatcaccgt ctccagatag acaggtctgc cacgataggc gatccagcgg
     5581 cacatttctt tcagttccga agcgaaacac gaaaaatgca ccctattgcg ggcacaaggt
     5641 ccgcgcaact ggcccggcgt ctactcgccg cactggaagt cgatcttgac cgccttgcgg
     5701 ctgcgcccga cacgcgcctc gatttccagc ggtgcgcagg tggcgttctc cagcatcacc
     5761 gccttgtgca cgatgccgct ctcgccgaac agcaggccgg cgaaggcccg gttcatgagg
     5821 atcttcggtc cggtcttggc cgtctgggtc accttgatgg cggcgagatc gcgggccagc
     5881 ttgtcggagg cgcggctgtt cttcggcccc tggaagacga gggtcacgag caccgcttcc
     5941 gccggttcac ccgcatcccc cgcgccgatc ggtgtattga acagcgcctt cttggagccg
     6001 accaggtttt cggaaagggt gccggtccgt tccaggaaca gctggacctt gacgtcctgc
     6061 acggtgatgc cctgtccggc ctgctgcgcc gcgacaggag cggatgcgag tgcgagcagc
     6121 gttgccgccg caagaacatg tttcattgaa ttgcccctct gctagcctac attcgtgcca
     6181 aaacgtcctg ttggccggtt cgcgcaacga cagcatctac ttggggaatc atgaacagga
     6241 tatatctgct cgccgcagcg gtttgcattc ccggtggtcc ggcgctggcc gccggagggc
     6301 tggacggcag ggcgcttggc atcgcctggg cattgccctt cttcggcctg ctggcatgca
     6361 tcgcgctcgg acccttgttc ttcgcccgca cctggcacca ccattatggc aagatagccg
     6421 cggtctgggc ccttgtaacg ctgctgccgc tggcggcgtt gcatggcgtc ggccccgcgg
     6481 tggaagtgct gctgcatgcg ctgcttgtcg agtatgttcc cttcatcatc ctgctgttcg
     6541 cgctctacac cgttgccggc ggcatcctga tttcgggcac catcaccggc acgcccggaa
     6601 cgaacacggc cattctggct ttcggcaccg gccttgccag tgtcgtggga acaaccggcg
     6661 catcgatgat tctcatacgg ccgctgatcc gcgccaacga tcagcgccgc cacaacgtgc
     6721 atgtcgtgat ctttttcatc tttctggtgt cgaacatcgg cggagcgctg acgccgctgg
     6781 gcgatccccc cctgtttgtc gggtttctgc gcggcgtcga cttcttctgg acgacgcggc
     6841 atctgctcgc gccgatgctg ctcctctcgg tcctgctgct tgccgggttc tatctgctgg
     6901 atcgctggtt ctggcagcgc gatgccggcc ttcctccgct gaaggatttc acgccggaac
     6961 gttccgtcaa gctcttcggc aagcggaaca tcccgctgct ggcggccatc atcgctgtcc
     7021 tcttgctcac atccgcctgg aagccgggga tcaccgttcc ggttcccggc gcgccgatgc
     7081 cgttgcaggg gcttgtcagg gacatcctgc tgctggtcat cgcgggcctg tccttgcgtc
     7141 tgacgcctgg accggtgcgc gccggcaacg agttttcctg ggagccgatt gccgaggtcg
     7201 gcaagctgtt cgcggccatc ttcgtctgcg ttgctccggt gatcgctatg ctgcaggtcg
     7261 gaaaggaggg cgttttcgcg cctctggtcg ccttcgtgac atcgtccggg ggccaaccga
     7321 ttccccaggc ctatttctgg ctgaccggat tgctgtcggc gtttctggat aacgcgccga
     7381 cctatctcgt gttctttgag ctggcgggcg ggaatgccgc cgacctcatg accgcgaagg
     7441 ggcagaccct tctggccatt tcctgtggtg cggttttcat gggtgcgctg acctatatcg
     7501 gcaatgcgcc gaacctcatg gtcgccacca tcgcccgaga catgaagatc cagatgccgg
     7561 ggttcctcgg tttcatggcg tggtcgttcg gaatcctcgt cccgctcttc ctgctcgtgg
     7621 gttggctctt catctgagtc gccgaaattt cctgttcaaa cacccgcggc acttgcgcta
     7681 aggacggtgc gggacctggg aggaagaaca tgccgctgat tgcgcggatg gcgccgctgg
     7741 cgctgacgat catttcatcg cctgtgatgg ctgcggaagg gctggacggc aagagtttcg
     7801 gtatcggctg gtcgctgcct ttcgccggcc tccttctcac catggccctg gcccccctgt
     7861 tcctgcatcg cacctgggaa caccactacg gcaagatcgc cgccggctgg gcactggtgg
     7921 tgttgattcc gctggcgttg acacgcggtt tcggcgtgac gctggaagcc ttcctgcacg
     7981 cgatgctggt cgagtatatg ccgttcatta tcctgctgtt tgcgctctac acgatcgcgg
     8041 gcggtatcct cgtgaccggc aatctgcgcg gcactccggc cacgaacacc acgcttctgt
     8101 tgatcggcac gatcctcgcg agtttcgtcg gcaccaccgg cgcctcgatg atcatgatcc
     8161 gcccggtctg ccgcgccaac gacgaccgcc ggcacaacgt gcatgtcatc atcttcttca
     8221 ttttcctcgt ctcgaacgtc ggcggctcgc tgacgccgct gggcgatccg ccgctgttcg
     8281 tcggcttcct gcgcggggtc gacttcttct ggacgacgaa agcgctgttc tggccgaccg
     8341 tgttcgtggc ggctgtgctg cttgcggcct tctatgcgct ggatacctat ctgcacgcga
     8401 aggacgacaa gctgcctgcg ttgaaggaca agaccccgga tcgggacatc aagatcttcg
     8461 gccaggtcaa tttcctgctg ctcgcgggtg tcatcgccgc catcctgatt tccgcttcct
     8521 ggaagccggg tgtgagcctc aagctgcccg gcgcgaacct tccggtgcag ggcctggtcc
     8581 gcgacgccgt cctgctggct ctggccggtc tgtcgctgac cctgacgccc aagcaggtgc
     8641 gcgccggcaa cgagttttcc tggggcgcga tcctggaggt cggcaagctg ttcgcggcca
     8701 tcttcgtctg cgtcattccg gtattggcga tgctgcaggc cggacatcag ggcgcgctga
     8761 aggggctcgt cgcgcttgtc agcaacgctg acggctcgcc aaatccgcag atgtactatt
     8821 tcctcaccgg catcctgtcg tcgttcctcg acaacgcgcc gacctacctt gtgttcttcg
     8881 agctggcggg cggcgacccg caggtcctga tgacgcagaa ggccaacatt ctggcggcca
     8941 tctcgctggg cgcggtctac atgggggcga acacttacat cggcaacgcg ccgaacctga
     9001 tggtggccaa catcgccagg gacatgggcg tgaagatgcc ctcgttcctc ggctacatgg
     9061 tgtggtcctt cgccatcctc atgccggtgc tggtgctcgt cggcttcgtg ctggtcaagt
     9121 agccgtggga gccgggggga ggggcgctca gcccagcccc tccggcttcg gtccgccggt
     9181 cgcccagtcg aggagttcga ccgtgtgaac gatggcgcgg gtttcgccgc gctcctcgaa
     9241 acccttggcg atctgcgtca tgcagccgag gttgccggtg gcgatgacgg gcgcgcccgt
     9301 gcgttcgata ttggtgacct tgcgcgcgcg caggctgcct gagatttccg gctggagcat
     9361 gttgtaggtg ccggcggaac cgcagcagat gtggccttcc ggcacgtcct tcaccgtgaa
     9421 cccggcctgg cgcagcagcg tcttcggcag gtccgtgatc ttctgcccgt gctgcatcga
     9481 gcaggcggag tgataggcca cggtcagccc ggggcccgga cggacggaca ggtccagctt
     9541 cgcggcgaat tcggtgatgt ccatcgccag tgcggccacg cgcgcagcct tgtcggcata
     9601 ggccggatcg tcgcggaaca tgaagccgta gtccttgatc gtcgtgccgc agcccgaggc
     9661 ggtaatcagg atggcgtcga ggccaccgtt cgcgatctcg gcggtccaca ggtcgatcat
     9721 ccgccgcgcc tgctcatggc tttcgtgctc gcggcccatg tggtggacga gcgcgccaca
     9781 gcagccctcg ttcctcggca cgatcacctc gtagccgtgg cgcgtgacga gccggatggc
     9841 ggcgtcattg atgccggggg caagtacgct ctgggcgcag cccgacagca gcgcgacgcg
     9901 gcccttgcgc ggacctggag gcgcatgaac gcccggcccg tccgtcggcg tgcggttcgg
     9961 caggcgcgcg ggcgccagac cgagcatg
//
LOCUS       DAOMXN010000079         9964 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_3586_length_9964_cov_12.202644, whole genome shotgun sequence.
ACCESSION   DAOMXN010000079 DAOMXN010000000
VERSION     DAOMXN010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 9964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9964
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3586_length_9964_cov_12.202644"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            232..600
                     /locus_tag="PK812_07305"
     CDS             232..600
                     /locus_tag="PK812_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69400.1"
                     /translation="MALGTRRDFPTTRRLAAILAIASGGLLAGCAAQHQVTGVVEGGN
                     EVFTGQAIRSPDLAGSLVVVSNRGVQCVGNYVFTEPRMGAGTFRCSDGRTGPFKFVST
                     GLRGTGTGRLGDKTFTFSFG"
     gene            complement(646..1515)
                     /locus_tag="PK812_07310"
     CDS             complement(646..1515)
                     /locus_tag="PK812_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRJ69401.1"
                     /translation="MLRLFASRPTLAGIAFMCLGIAMFSLNDVMGKWLLGTYTVGQLL
                     LIRSVAALAVLTPAILKEGVANVARPPHPLLQVLRVVLATLEVAFFYWAVSYMPLAET
                     VTFYLAGPIYVALLARPLLGETIPMRRWAAIALGFLGVVLALNPGGATFGWPALIAFL
                     GSFGFALMMIVTRMVRGTSDTTLVAWQTVAALIAGAVLSPFSWTTPSVRDFLLLGLLG
                     IVAMFAHMATNRSLKLAPANVVVPYQYTLIVWAVVLGWLVFGDIPAWTTLAGAAIIVV
                     AGFWIFFDERRAS"
     gene            complement(1547..2266)
                     /locus_tag="PK812_07315"
     CDS             complement(1547..2266)
                     /locus_tag="PK812_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012254862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HRJ69402.1"
                     /translation="MSSDFSLWVYLSQKPLTWLTLTLIAYAVADRLAAATKRHPLANP
                     VLLAVALVGIVLFASGTPYRQYFEGAQFVHFLLGPATVALAVPLYEHRAEVLRSLLPM
                     AAALIAGAVVTLASVIVIMVLLGAPLPVIASIAPKSVTAAIAMGVSEGLGGDPALTAV
                     LVILTGVFGAIIVTPLLDSAGIQDWRARGFAAGIAAHGIGTARALQVNPVAGTFAAIA
                     MALNAVFTALLTPLALKALSG"
     gene            complement(2263..2640)
                     /locus_tag="PK812_07320"
     CDS             complement(2263..2640)
                     /locus_tag="PK812_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HRJ69403.1"
                     /translation="MIVALSVLLACQLAGEVLARGLGVPVPGPVLGLIILLMALMARR
                     GLAALMPAEVNDGTLEAAAKALLAHLSLLFVPAGVGVIQRLDVLGQHALALGLALVLS
                     SILGILASAMVFRWVARCLEKRA"
     gene            2985..3197
                     /locus_tag="PK812_07325"
     CDS             2985..3197
                     /locus_tag="PK812_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012321928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HRJ69404.1"
                     /translation="MAKGTVKWFNTQKGYGFIQPEAGGPDVFVHITAVQRAGLRELRE
                     GDKLSYELVTDRRTGKTSAGELQLAQ"
     gene            3236..4138
                     /locus_tag="PK812_07330"
     CDS             3236..4138
                     /locus_tag="PK812_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012456687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HRJ69405.1"
                     /translation="MREGLLPNNATHLLRLEAEERVARRAADFLTEMLPPEETAAAAF
                     EIDDGRTWSLEVYFAQAPDEVTVRSLLAQCAGDTQAQSATFSRIEQQDWVAAALEGLA
                     PVRAGRFLVHGSHDRRRVRGNDIGIEIEAALAFGTGHHGTTRGCLLYLDRILKVRKPQ
                     RVLDVGTGTGLLAIAAARALHVPIMAGDIDPVSTMTARANARANGAGAYVRPVTATGV
                     RHPVLMTEAPYDLVFANILARPLMKLAASIARVTARPGTLILSGLLERDVNGVLSAYR
                     MQGFRLIARQNLEGWVSLMLRRGR"
     gene            complement(4203..4418)
                     /locus_tag="PK812_07335"
     CDS             complement(4203..4418)
                     /locus_tag="PK812_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69406.1"
                     /translation="MAIATRSFDFDYAFMPFGLADRRPAPRPGFWKKLYDAMLDSRRI
                     AAEREIRRHGAMLQRVEQAASRGELPF"
     gene            4660..6480
                     /locus_tag="PK812_07340"
     CDS             4660..6480
                     /locus_tag="PK812_07340"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HRJ69407.1"
                     /translation="MTKSRLQKFTERGGGRHCRSRLSALRTELGARGLDGFIVPRADE
                     HQGEYVPPGDERLAWLTGFTGSAGVAVALADKAALFIDGRYTEQAAAQTDTDAFAPVA
                     IHQVGVGEWLSQHARPSARIGYDPALHTPGEIDRLTKTLTPYAIALEATAPNPLDAVW
                     LDRPSPPLAKVIEHPERLAGEPAEAKLNRIREALSRERLDALVVSDPHALAWAFNIRG
                     GDVGHTPLPLGYGIIPAEGIPALLLDGRKLSNSMRAKLAEFADIREPADLSSAVATAA
                     QGGRRIRLDAATAGVRLRSWVEAAGGIADLGPDPIALMKARKNAAEQAGARAAHLRDG
                     VAVTRFLAWFDREAPKGLLTEIDAAVALEGFRRDTGALKDLSFPTISAANAHAALPHY
                     RVSTKSNARIEAGIYLVDSGAQYQDGTTDITRTLSVGAPTREMRERNTRVLKGMIAVS
                     VAVFPKGISGAQLDSFARVALWEAGLDFDHGTGHGIGSYLSVHEGPQRISKLGTTALE
                     PGMMLSNEPGYYKPGAFGIRIENLVLVEERRIQGAERVMYGFETLTLVPIDRRLIVKS
                     MLTRRERDWLDAYHARVQAALKPHLDRSEARWLTAATAPL"
     gene            6488..7699
                     /locus_tag="PK812_07345"
     CDS             6488..7699
                     /locus_tag="PK812_07345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HRJ69408.1"
                     /translation="MSHYTTGPKHSGVRLRPMLPLLLLASMVAPLTTNILLPSLPGLA
                     KSLDVSREAAQLAISAYIFAMAFTLLLIGPVSDRFGRRPTLLACFAVFTVASLMAIFA
                     TSIEMLVAARLLQALGATAGMSVPRTVVSDVSSRSETARIIAYMTAVMVLAPMAAPNV
                     GSLLDSLFGWWSIFAFCAGLGALVFALAALTMKETRPQGATPPTAGEILDRSKSLLRN
                     REFLRFAALTSTSTASYYALLGGAPDLIIDGMGRTPAEFGRWLIMLGIGYAAGNFLSG
                     RIAGRVGLVRMAALGNMVLLLSGVLMVVGALAGLHVPLAVFLPALIMAFGNGLVLPST
                     MASALQIDPRAAGTASGLMGFIQMFSSAVMAQAIVSFGGKTALPIGLAIAISGLIAVV
                     LLPPRFRQGPR"
     gene            complement(7790..9883)
                     /gene="ligA"
                     /locus_tag="PK812_07350"
     CDS             complement(7790..9883)
                     /gene="ligA"
                     /locus_tag="PK812_07350"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HRJ69409.1"
                     /translation="MALETLTPLEARAEHAALTAEIAEHDRRYFQEDAPSIADAEYDA
                     LRRRLDAIEVAYPEFKSADSLTQRVGAAPSGKFAKIRHAVPMLSLANSFSAEDVADFV
                     GRIRRFLDLGEDAPLALTAEPKIDGLSCSLRYEKGRLVSAATRGDGAEGEDVTANVRT
                     LKEIPHHLAGTGWPEVAEVRGEIYLGHADFAALNASQAAAGEKVFANPRNAAAGSLRQ
                     LDASITASRPLRFFAYAWGEMSAMPASSQSGMVAVFGRWGFRTNPLMTVCRSVEELLS
                     HYRRIEAQRATLGYDIDGVVYKVDDLALQARLGFVARSPRWATAHKFAAEQATTILDA
                     IDIQVGRTGAHTPVAKLMPVTVGGVVVSNATLHNEDEIARKDIRVGDTVVVQRAGDVI
                     PQVVEVVLDKRPTGSAPFVFPHVCRCELKTPLVRETTAQGEAGAVRRCTGEFACPFQR
                     IEHLKHFVSRRAFDIEGLGEKQIAFFYEKGLIREPADIFTLEARDKASLTRIQNYEGF
                     GETSVRNLFAAIAARREIALERFIFALGVRHIGESTARVLARAYGSWLAFGAACRRIA
                     ADEPEAKAEMDNLDQIGEAVVEALGDFFREPHNAGMVERLAAEVVVLDAEKPKTDTAV
                     GGKTVVFTGSLERMTRDEAKARAESLGAKVAGSVSKKTDIVVAGPGAGSKLDKAREFG
                     VRVLTEDEWLALIAG"
BASE COUNT         1777 a         3438 c         3129 g         1620 t
ORIGIN      
        1 gacagggcat gtcccgccgg cctcagcccc gcgccgccct gtcgccaccc cgccgatatt
       61 ccggcagggc cgtttccgct cgtctatgcc ttgccggaaa ggagccgccg ccggaactgg
      121 ccgcatcgcc ttttttcccg cgcaattcgg cccgtttctt ccgagaatcc cgcccgaatt
      181 cggccttcca ccagccacac gatgctccgg ggcccacaat caggagacat catggctctc
      241 gggacgagac gggactttcc aacaacgcgg cgactggctg cgatccttgc catcgccagc
      301 ggcggccttc tggccggctg cgcggcgcag catcaggtga ccggcgtggt cgaaggcggc
      361 aacgaggtgt tcaccggcca ggcgatccgt tcgccggacc tggcgggatc gctggttgtc
      421 gtgtccaacc gcggcgtgca gtgtgtcggc aattatgtgt tcaccgagcc gcggatgggc
      481 gccgggacct tccgctgctc ggacgggcgt accggaccct tcaagttcgt gtcgacaggc
      541 ctgcgcggca ccggcaccgg ccgcctcggc gacaagacct tcaccttcag tttcggctga
      601 ggcaccgcgt cggggcgaag ccctcatcgg ctttgccccg gagcttcagg acgcgcgccg
      661 ttcgtcgaag aagatccaga atccggcaac gacaatgatc gccgcaccgg ccaacgtcgt
      721 ccaggccggg atgtcaccga acaccagcca gcccagcacc acggcccaga cgatcaacgt
      781 atactgatac ggcaccacca catttgccgg cgccagcttg agcgaacggt tggtggccat
      841 gtgggcgaac atcgccacga tcccgagcag acccagcagc aggaagtccc gcacggacgg
      901 cgtggtccag ctgaagggcg acagaacggc gccggcaatc agcgctgcca ctgtctgcca
      961 cgccaccagc gtggtgtcgc tggtgccgcg caccatccgc gtcacgatca tcatcagagc
     1021 gaagccgaag ctgccgagaa aggcgatcag cgccggccag ccgaaggttg ctcctccggg
     1081 gttcagcgcc aggaccacgc ccaggaagcc cagggcgatg gccgcccagc gacgcatcgg
     1141 gatggtctct cccagtagcg gacgagccag caacgcaacg tagatcggtc cggcaagata
     1201 gaaggtgacc gtctcggcca acggcatgta cgacaccgcc cagtagaaga acgcaacctc
     1261 cagcgtggcg agaaccacgc gcaggacctg caacagcgga tggggtggcc gcgccacatt
     1321 ggccacgccc tccttaagaa tggccggcgt caataccgcc agcgccgcga ccgaacggat
     1381 cagcaggagc tggccgacgg tataggtgcc aagcagccac ttccccatca cgtcgttcag
     1441 ggaaaacatg gcgatgccga ggcacatgaa ggcgatgccg gcgagcgtcg ggcgggaagc
     1501 gaacagacga agcatgctgg gtgagtcttt ttcggatttc ctgcgcttag cccgagagcg
     1561 ccttgagggc cagcggcgtc agcagcgcgg tgaacacggc gttgagcgcc atcgcgatcg
     1621 cggcgaaggt gccggccacc gggttgacct gcagagcccg cgccgtgccg atgccatgcg
     1681 ccgcgatgcc cgcggcaaag ccacgcgcgc gccaatcctg gatgccggcg gaatccagca
     1741 gcggcgtcac gatgatggcg ccgaagacgc cggtcagaat gaccagaacc gcggtcagcg
     1801 cggggtcgcc gccaagccct tccgaaacgc ccatggcgat ggcggccgtc acggacttcg
     1861 gcgcgatcga ggcaatcacc ggcagcggcg cgcccagcag gaccatgatg acgatgacgc
     1921 tcgccagcgt caccacggcg ccggcgatca aggcggccgc catcggcagc aggctgcgca
     1981 gcacttcggc ccgatgctca tacagcggca ccgccagcgc tacagtcgcc ggccccaaca
     2041 ggaagtgcac gaactgcgcc ccttcgaagt actgccggta gggcgtgccg ctggcgaaca
     2101 gcacgatccc gaccagcgcg acggcgagca ggaccgggtt ggcaagcggg tggcgcttgg
     2161 tcgccgcggc caagcggtcg gcgacggcat aggcgatcag cgtcagggtc agccacgtca
     2221 gcggcttctg cgacagatat acccagagcg agaaatcgct gctcatgccc gtttctccag
     2281 gcaccgcgcc acccagcgga agaccatggc cgaggcgagg atgccgagaa tgctggacag
     2341 gaccagcgca agaccgagcg ccagcgcatg ttgccccaat acgtccagcc gctggatgac
     2401 gccgacgccg gccggcacga acagcagcga cagatgcgcc aacaacgcct tggcggcagc
     2461 ttcgagcgtg ccatcgttga cttcggccgg catcagcgct gccagaccgc gccgcgccat
     2521 cagtgccatg agcaggatga tcagaccgag caccggcccg ggcacgggaa cgccaaggcc
     2581 gcgcgccagc acctcgccgg ccaactggca tgccaacagc acggacaacg cgacgatcat
     2641 gccatttgca cccttagttg cgacaaacct gcggagcagg aaatgccatc ccaccagcac
     2701 ccggcgaagc ctgaaaaaat cgactgcgca ggctccggcc catggaagaa atcatttcca
     2761 acggccgggc catttcgaca agacccgcct cattgcaaga agctcaaaaa aagagtaggt
     2821 taacatatcg actttggccg gaatatcggg ccgcactgcc tgttggcttc catcggcgcg
     2881 cgttcagacc agcttcccca agttcgacca caccggggca ggcgattccg ctctgaacca
     2941 cgaccaactg gccccgaggg gccaccgaag gaaagacgac gactatggcc aaaggcactg
     3001 tcaaatggtt caatacccag aagggctatg gcttcattca gcccgaagcg ggtggaccgg
     3061 acgtgtttgt gcacatcacc gcggtgcagc gcgccggcct gcgcgagctg cgcgagggtg
     3121 acaagctctc ctacgagctc gtgaccgacc gccgcacggg caagacctcg gccggcgaac
     3181 tccagctcgc acagtaatgc tggacgcaat ccgacgcggc cgctaatccg gccgtatgcg
     3241 cgaaggattg ctccccaaca acgcgaccca cctcctccgc ctcgaggcgg aggaacgggt
     3301 tgcccgccgg gcggccgatt tcctcacgga aatgctgcca ccggaagaaa ctgccgctgc
     3361 ggccttcgag atcgacgatg gtcgaacctg gtctctcgaa gtatatttcg cgcaggcgcc
     3421 cgacgaggtc accgtccggt ccctgctggc gcagtgcgca ggcgacactc aagcgcaatc
     3481 agccaccttt tcccggattg aacagcagga ctgggtcgcc gccgctctgg aagggctggc
     3541 gccggttcgt gccggccggt tcctcgtgca tggatcgcat gatcgccggc gcgtgcgcgg
     3601 caatgacatc gggatcgaga tcgaggccgc tctggccttc ggcaccgggc accatggaac
     3661 gacgcgcggc tgccttttgt atcttgatcg catcttgaag gtgcgcaagc cccagcgcgt
     3721 gctcgatgtc gggacgggca ccgggttgct cgccatcgcg gccgccagag cgctgcatgt
     3781 gccgatcatg gccggggaca tcgatcccgt ttcgaccatg acagcgcggg ccaatgcacg
     3841 tgccaacggc gccggcgcct atgtccgccc ggtgacagcg accggtgtgc ggcaccccgt
     3901 gctcatgacg gaagcgccct acgatttggt gttcgccaac atccttgcac ggccgctgat
     3961 gaagctggcc gcatcgatcg cccgggtgac ggcacggccg ggcacgctca tcttgtccgg
     4021 ccttctggaa cgcgacgtga atggcgtgct gtcagcctac cggatgcagg ggttccgtct
     4081 catcgcccgt cagaacctgg aaggctgggt cagtctgatg ctcaggcgcg gccgctaagc
     4141 agccgcgccc gcggtcgatg ggaaaattgg cgcacggcgc cacgcggtat gcgcttctgc
     4201 gatcagaacg gcagctcgcc cctggacgcc gcctgctcta cccgctgcag catcgcgcca
     4261 tggcggcgaa tctcacgctc ggcagcgatg cggcgcgagt ccagcatcgc gtcatacagc
     4321 ttcttccaga agcccggacg cggtgcgggc cgccggtcgg caaggccaaa cggcataaag
     4381 gcgtagtcaa agtcgaaaga cctggttgcg atagccatgg gtcatgctcc tgttgatgga
     4441 ctggagtgcg atcccgcagc gtttcctcgt ggggaaagaa taagcgcttt ttcgcggatc
     4501 tgagagaggc tgagcgcatt caagtcatgc gaaaaatgca tagataattg aaaatttgtt
     4561 cacactcagg ccgcctaaac atatctcttt ctgccccatg gcctgaaaaa tccttgggcc
     4621 ttcggttgcg gaaggggagc aagcggatag tgttccgcga tgaccaagtc ccgacttcag
     4681 aaattcaccg agcgcggcgg cggcaggcat tgccgcagcc gcctgtccgc cctgcgcact
     4741 gaactgggcg cgcgtggcct ggatggtttc attgtcccgc gcgcggacga gcaccagggc
     4801 gagtatgttc cgcctggcga tgaacgtctg gcctggctga ccggattcac cggttcggcc
     4861 ggcgtggcgg tggcgcttgc ggacaaggcc gccctgttca tcgacggccg ctataccgaa
     4921 caggctgcag cgcagaccga caccgacgcg ttcgcgcctg tcgccatcca tcaggtcggt
     4981 gtcggcgaat ggctgtcgca gcacgcaagg ccgagcgcgc gcatcggata tgatcccgcc
     5041 ttgcatacgc cgggcgaaat cgatcgcctc accaaaaccc tgacgcccta tgcgatcgcg
     5101 cttgaggcga ccgcccccaa tccgctggat gcggtttggc tggatcgccc ttcgccgcct
     5161 ctcgccaagg tcatcgagca tccggaacgg ctggccggcg aacccgccga ggccaagctc
     5221 aaccgcattc gcgaagcctt gtcgcgcgag cgattggatg cgctggtcgt gagcgatccc
     5281 catgctcttg cctgggcctt caacattcgc ggcggcgatg tcggacacac gcccctgccg
     5341 ctgggctatg gcatcattcc ggccgaaggc atacccgccc tgctgctgga tggccgcaag
     5401 ctcagcaaca gcatgcgtgc gaagcttgcg gaattcgccg acatccgtga gccggccgat
     5461 ctttcctctg ccgtcgcgac cgccgcgcag ggcggcaggc gcatccgcct tgatgccgcc
     5521 acggccggcg tgcgcctgag gtcgtgggta gaagctgccg gcggcatcgc cgaccttggc
     5581 cccgacccga tcgcgctgat gaaagcgcgc aagaatgcgg ccgagcaggc cggcgcacgc
     5641 gcggcccacc tccgcgacgg cgtggccgtg acccgcttcc tggcctggtt cgaccgtgaa
     5701 gcgccaaaag gtctgctcac cgagatcgac gcggcggtgg cactggaagg attccgccgc
     5761 gacaccggcg cgctgaagga cctctcgttc ccgaccatct cggccgccaa tgcccatgcc
     5821 gcgctgccgc actaccgcgt gtcgaccaaa agcaatgctc ggatcgaagc cggtatctat
     5881 ctggtggatt ccggagcgca atatcaggac gggaccacgg acatcacgcg cacgctctcg
     5941 gtcggcgcgc cgacgcgaga aatgcgtgaa cgcaacacca gggtcctgaa aggcatgatc
     6001 gccgtctccg tggcggtgtt tcccaagggc atctcgggcg cacagctgga cagctttgcc
     6061 cgcgtcgcac tctgggaggc gggtctggac ttcgaccacg gcacgggtca cggcatcggc
     6121 agctatcttt cggtacacga gggaccccag cgcatatcca agctgggcac caccgcactc
     6181 gagccaggca tgatgctgtc caacgagccc ggctactaca agcccggcgc cttcggaatc
     6241 cgcatcgaga acctggtcct ggtcgaggaa cggagaattc aaggcgcgga gcgcgtcatg
     6301 tatggcttcg agaccctcac cctcgtgccc atcgaccgca ggctgatcgt caagtccatg
     6361 ttgacacggc gcgagcgcga ctggctggat gcctaccacg cgcgtgtcca ggcagcgttg
     6421 aaaccgcacc tcgaccgcag cgaggcacgc tggctcaccg ccgcgacagc gccgctctga
     6481 ggccgaagtg agccactaca caaccggtcc gaagcactcc ggcgtgcgcc tgcgtccaat
     6541 gctgccgctg ttgctgttgg cctcgatggt ggcgccgttg accaccaaca tcctgctgcc
     6601 atccctgccc ggactggcga aaagccttga cgtctcgcgc gaggcagccc agctcgcgat
     6661 ctcggcctac atcttcgcca tggctttcac gctgctgctg atcgggccgg tgtcggatcg
     6721 cttcggccgc cggccgacgc tgctcgcgtg ctttgcggtg ttcaccgtgg caagtctcat
     6781 ggcgatcttc gccaccagca tcgaaatgct ggtcgctgcg cgcctgctgc aggcattggg
     6841 cgccacggcc ggcatgagtg tgccgcgcac ggtcgtcagc gatgtttcca gccgttccga
     6901 gactgcccgg atcatcgcct acatgactgc ggtgatggtg ctcgctccga tggccgcgcc
     6961 gaatgtcggc agcctgctcg attccctgtt cggctggtgg tcgattttcg ccttctgtgc
     7021 cggactgggc gcgctcgtgt ttgccctcgc cgcgctgacc atgaaggaaa cccgaccgca
     7081 gggcgcgacg ccgcccacgg cgggggagat actggaccgc agcaagagcc tgctgaggaa
     7141 ccgcgagttc ctgcgcttcg ccgcactcac gtcaacctcg accgcctcct actacgcctt
     7201 gctcgggggc gcgccggacc tcatcatcga cggcatgggc cgcacgccgg ccgagttcgg
     7261 gcgctggctg atcatgctgg gcatcggcta tgccgccggc aacttcctgt ccggccgcat
     7321 cgccggaagg gtcgggctgg tgcgcatggc ggcgctcggc aacatggtgc tgctgctgag
     7381 cggcgtgctc atggtcgtcg gcgcgctggc ggggctgcac gttccactgg cggtcttctt
     7441 gccggcactg atcatggcat tcggcaacgg gctcgtgctg ccgagcacca tggcgagcgc
     7501 cctgcagatc gacccgcgcg ccgccggcac cgcatccggt ctgatgggct tcattcagat
     7561 gttctcgtcc gccgtcatgg ctcaggccat tgtctccttt ggcggcaaga cggcacttcc
     7621 gatcgggctg gcgatcgcca tttccggact gatcgccgtc gtgctgctcc caccacgctt
     7681 caggcaggga ccccgctgag cgacgcggtg gaaagagcaa ggggcattcc gggctttgcg
     7741 tcccggctcc ggatggactc ccgcgcaaac actcttgcgc caaccggctc taccccgcga
     7801 tcaacgccag ccattcgtct tcggtaagca cccggacgcc gaattcacgc gctttatcga
     7861 gcttcgagcc cgcgccaggc ccggcgacaa cgatgtcggt cttctttgac accgaaccgg
     7921 caactttcgc cccgaggctt tccgcccgcg ccttcgcctc atcgcgcgtc atgcgttcga
     7981 gcgagccggt gaagaccacg gtcttgccac caaccgccgt atcggtcttc ggcttttcag
     8041 catcgaggac aacaacctca gcggcaaggc gctccaccat cccggcattg tgcggctccc
     8101 ggaaaaagtc gccgagcgcc tcgaccacgg cctcgccgat ctggtccaga ttgtccattt
     8161 cggcctttgc ctcgggctca tcggccgcga tgcgccggca ggctgcgccg aaagccagcc
     8221 aggacccata ggcccgggcc agaaccctgg ccgtgctctc gccgatatgc cgcacaccca
     8281 gcgcgaaaat gaagcgctcc agcgcaatct cccgtcgcgc cgcgatggcc gcgaacaggt
     8341 tgcgcacgct cgtctcgccg aacccctcgt agttctggat gcgcgtgagc gaggccttgt
     8401 cccgcgcctc cagcgtgaag atgtcggccg gctcgcggat cagccctttc tcgtagaaga
     8461 aggcgatctg cttttcgccc agtccttcga tatcgaaagc ccggcgtgag acgaagtgct
     8521 tcaggtgctc gatgcgctgg aacggacagg cgaattcccc cgtgcagcgg cgcaccgcgc
     8581 cggcttcgcc ctgcgcggtc gtctcgcgca cgagcggcgt cttcaactcg cagcgacaga
     8641 catggggaaa cacgaacggc gcggaccctg tcggccgctt gtcgaggacc acttccacca
     8701 cctgcggaat gacatcgccg gcccgttgca ccacgaccgt gtcaccgacg cgaatgtcct
     8761 tgcgggcaat ctcgtcctcg ttgtgcagcg tggcgttcga gacgacgaca ccgcccactg
     8821 tcaccggcat cagcttggcc accggcgtgt gcgcaccggt gcgccccacc tggatgtcga
     8881 tggcgtcgag gatggtcgtt gcctgttcgg ccgcgaactt gtgcgccgtc gcccagcgcg
     8941 gcgagcgcgc cacgaaaccg agccgcgcct gcaatgccag gtcgtccacc ttgtagacga
     9001 cgccgtcgat gtcgtagccc agcgtcgccc gctgcgcctc aatccgacgg taatgcgaca
     9061 gcaactcctc caccgagcgg cagaccgtca tcagcggatt ggtgcgaaag ccccagcgcc
     9121 cgaacaccgc caccatgccg ctctgggacg aggctggcat ggcactcatc tcgccccagg
     9181 cataggcaaa gaaccgcagc ggtcgcgatg cggtgatgct cgcatcgagc tgccggagcg
     9241 accccgccgc cgcattgcgc ggattggcga agaccttttc gcccgccgcc gcctgcgacg
     9301 cgttcagcgc cgcgaaatcg gcatggccga ggtagatctc cccacgcacc tccgcgacct
     9361 ccggccagcc tgtgccggcc aggtggtgcg gaatctcctt gagcgtgcgg acattggcgg
     9421 tgacatcctc gccttccgca ccatcgcccc gcgtcgcggc ggaaaccagt cgccccttct
     9481 cgtagcgcag ggagcaggac aggccgtcga tcttcggctc ggcggtgagt gccagcggcg
     9541 catcctcgcc gaggtcgagg aagcgccgga ttcggccaac gaaatccgcc acgtcttctg
     9601 ctgaaaagct gttcgccaac gacagcatcg gcaccgcatg ccggattttc gcgaatttgc
     9661 ccgaaggcgc cgcgccgacg cgctgggtca ggctgtcggc gctcttaaac tcgggatatg
     9721 ccacctcgat cgcgtcaagg cgccggcgca gcgcatcgta ctccgcatcc gctatgctcg
     9781 gcgcgtcttc ctggaagtag cgccggtcat gctccgcgat ttcagctgtc agagcagcat
     9841 gctcagcccg tgcttccagc ggtgtcagcg tctccagggc catgaatcgg cggttcgcca
     9901 cgctcctacc cccctgccag cagccgatca gccgccgcac gcgcctcggc gctgatcgcc
     9961 tcgc
//
LOCUS       DAOMXN010000080         9934 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_3599_length_9934_cov_10.977427, whole genome shotgun sequence.
ACCESSION   DAOMXN010000080 DAOMXN010000000
VERSION     DAOMXN010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 9934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9934
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3599_length_9934_cov_10.977427"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="PK812_07355"
     CDS             <1..691
                     /locus_tag="PK812_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69410.1"
                     /translation="VRFWWRDDDAVEPGPALDALLALSAHFDAPISLAVIPQTASAGL
                     AARLAGDRRVDVLVHGIAHSNNAGAGVKKQELVSGGDETVRALALAWQRVSTLFGERA
                     LPILVPPWNRIAPDLVRRLPEAGFAGLSTYATMIASAPGRRIDTHIDPIDWKGGGGRV
                     GASAHIARLAAMLEESLDRNPPVLGPVGLLTHHLVHDPWVWALVRELLTVLSAHRAVR
                     FVAARELFGLT"
     gene            910..2784
                     /locus_tag="PK812_07360"
     CDS             910..2784
                     /locus_tag="PK812_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010682632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ69411.1"
                     /translation="MLERTLSVRDVAIDFVSDGHVTNAVKNISFDLAPGRTLAIVGES
                     GSGKSVTSQAIMGILSKNGRVTNGTIHFRDPKTGVITDITALDRDGEEMRKLRGGKIA
                     MIFQEPMTSLSPLHTIGDQIGEVVKLHEGADQATADRRTADLLGKVGFRDPERALKIY
                     PFELSGGMRQRAMIAMALVCNPAVLVADEPTTALDVTTQAQILELMRKLQREFKMAVL
                     LITHDLGVVANMADDVVVMYRGEVMERGPVQDIFRDGRHDYLKALLRSIPKFGMKEDE
                     RLVPIRAIKSQIAAEDRPTGPRAAGSGRKVLEVTGVSKYFTLRSGMFGSKKTTIQALS
                     NVSLAVPAGQTLGLVGESGSGKTTLSKIIMRAMTPDEGQIHYDAGQGPKDVAKLEGAE
                     LFAYRRAVQFVFQDPFSSLNPRMTCYELLSEPLRIHNIGTPEERFQRVKYLMEVVGLD
                     VRQLRRYPHAFSGGQRQRLGLARALALKPSVLLCDEPTSALDVSVQAQILNLLKDLQR
                     ELDLSILFVSHNLAVVDYMAQEIAVMCRGHIVEQAPRAALFGNPQHPYTKALLAAVPE
                     PDLDRPLDFGKVSGGFSNPAEWPAPFTIRPGDFHPRMAEVAPGHFVRIGEAASLDRAS
                     "
     gene            2793..4766
                     /locus_tag="PK812_07365"
     CDS             2793..4766
                     /locus_tag="PK812_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRJ69412.1"
                     /translation="MIASAMLPSSQGRPARGATKPSLLRGLRLECVAAAFLAMTAVAS
                     AADIRLIDPPFFKERVEKQQLPEIAQRVPKQPLVADLEARGRVPGRHGGDIRTLVSAV
                     RDLRYVTVYGYTRLIGYTEKLELKPDLLADIEIEGGRVFTLTLREGHRWSDGKPFTTE
                     DFRYYWEDMANDKSIAPAGPPEVYFVDGQLPKFEVLDERRVRFTWSKPNERLLPFWAQ
                     PGKVFSYAPAHYLKQFHAKYTPEADLKKRIADAKVRSWAALHNRMDDPYENGNVDMPV
                     LGPWRVLTKGPASRYVFERNPYYHRIDREGRQLPYVDRILVDIAAGGLIAAKANAGET
                     DLQGRGLSMVDAGVLKEGEKLNGYRTLLWPNARGSAYALYPNLNTADPEFKKLNRDLR
                     WRRALSHAIDRRMLNNALLFGLGREVGNTVEPQSPLYSEAAAKMHMAYDPALANRLLD
                     EIGLTQRDSLGFRLLPDGRRLELLVELNGGDGDMLDALQLIAETWADIGIKLIAKPQD
                     RSILRKRSYSGQTVMVMSTGLDNAVPTADMPPIELAPAVQDNYAWPMWGQHVETKGKS
                     GEPADIPAAKALLDDYTAWRSTNDPAQKTAIWKRMIENYAANLWTIGTVSGELQPVVV
                     SKKLRNVPDKAVFAWEPMALFGVHRIDEFFYAE"
     gene            4773..5795
                     /locus_tag="PK812_07370"
     CDS             4773..5795
                     /locus_tag="PK812_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ69413.1"
                     /translation="MLLYLLKRLATMIGTLFLVSALVFGIIKLPPGDFLTNQIAELKA
                     SGDAASVDKVEFLRKEYGLDKPAWEQYLVWIGLMPGAHGYSGLIQGDLGWSFEHQQPV
                     SAILGESLALTLIVNIAAIIFIHVLAIPAAVYSAKRQYSLPDYIITFIGYLGIATPSF
                     LLALVLLFYLNRWFGVSIGGLMDPKFEGQPWSLAKVGSVAAHLIVPTIVIGLAALASM
                     VRRMRANLLDELQKQYFVTAKAKGLDPNKALIKYPFRMSLNPFISDIGNMLPGLVSGS
                     ILVSLVMSLPTVGPKLLQALKVSDQYLAGSILMFVSLLTIIGMFISDLLLAMLDPRIR
                     FGAKGK"
     gene            5887..6978
                     /locus_tag="PK812_07375"
     CDS             5887..6978
                     /locus_tag="PK812_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009494019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ69414.1"
                     /translation="MPTGDEAYYRASTWTLMWAKFKRNKLAVISLFVLLAFYGSLVIV
                     EQIAPYAASKRHGDHLYAPPQSVHLFHQGEFRGPFVYPYAFKFNLETFKREYVIDETK
                     PQPIRFFCSGDSYDFMGVAKLDFHFVCPPKDGTMFLLGTDKLGRDLLSRIIYGARISL
                     TVGVVGIAISFIIGLTLGGLAGYLGGWVDAVVQRSIEILRSLPELPLWLALSAAVPPN
                     WSPVTVFLAITVILGFLDWPGLARAVRSKLLALREEDFVRAAELMGASKSRIIGRHLV
                     PNFMSHLIASATLSIPAMILGETALSFLGLGLRPPVTSWGVLLNDAQNLASVNLYPWL
                     LTPMIPVIIVILAFNFVGDGLRDAADPYH"
     gene            7051..7365
                     /locus_tag="PK812_07380"
     CDS             7051..7365
                     /locus_tag="PK812_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69415.1"
                     /translation="MQNHPFFGRRALFGAVGLVAALLAAPVAAQDPASDPVAALTPAQ
                     AYARGCGSCHRSERGVVRAIPKRPDAERRAWIETFMADHPCEFDAGKAKILDYLVEKS
                     RR"
     gene            7610..8326
                     /locus_tag="PK812_07385"
     CDS             7610..8326
                     /locus_tag="PK812_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007755288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease activity regulator RraA"
                     /protein_id="HRJ69416.1"
                     /translation="MPVESKPLDPKILAALKQCSAATVTTILLKKGVRTVWMRGTKPL
                     RADQPRTVGRAFTLRFVPVREDLATPESWSNPISTRAAIEAMPEGCLAVVDAMGVDDA
                     GIFGDILCARMVKRKVAGLITDGVVRDAEGVLSTGLPVWCNGVAAPASVAGLTFVGWQ
                     EPIACGGVAVFPNDVVVVDKDGAALIPEALLDYVAEHGPEQERMEAWIMKEVDKGLPL
                     PGLYPMNAETKARYEASKKA"
     gene            8402..8872
                     /locus_tag="PK812_07390"
     CDS             8402..8872
                     /locus_tag="PK812_07390"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HRJ69417.1"
                     /translation="MSSLELKYPPVAVFLVCAVLVPVLAFVFPGFGMSGGDPWKSAIA
                     SLVGSAGLMLGLAGVVQFRMHRTTVHPQHPERSSSLVTDGVYGFTRNPMYLGLALVLA
                     AFGVFYMLHLAGVLCTAAFVFWMNRFQIEPEERMMQEKFGAQYHAYSQRVRRWI"
     gene            8894..9817
                     /locus_tag="PK812_07395"
     CDS             8894..9817
                     /locus_tag="PK812_07395"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRJ69418.1"
                     /translation="MTASYAGVFPIAPTPFLPDGAIDWASAERLFAWYREIGSDGLTV
                     LGIMGEAPKLEPAESLRLLKLAVKHFAGLPVIVGVSAPGFAAMRSLAREALAAGAASV
                     MIAPPGTLRTDDSIVGYYAQAVEAIGADVPFVIQDYPLVTSVQMTPGVIRRIVQDQAS
                     CVMLKAEDWPGLEKITALRSWQKDGTLRDLAIMVANGGLFLDFEMERGADGSNTGYAF
                     PEMLIDVVRLSRTGQRDAAHDLFDAHLPLLRYEQQPNVGLAVRKYVMMRRGILAHDAQ
                     RKPGAGLSATARAEVDYLLARLARVDRRAAV"
BASE COUNT         1574 a         3334 c         3259 g         1767 t
ORIGIN      
        1 cgtccgcttc tggtggcggg atgacgacgc ggtggagccg ggaccggcgc tggatgccct
       61 gctggccctg tccgcgcatt tcgacgcgcc gatctcgctc gcggtgattc cgcaaaccgc
      121 gagcgccggt cttgccgcgc ggctggccgg cgaccggcgg gtggatgtgc tggtgcatgg
      181 catcgcgcac tcgaacaacg ccggcgcggg cgtcaagaag caggaactgg tgagcggcgg
      241 cgatgagacg gtccgggcgc tggccctcgc ctggcaacgc gtgtcgacgc tgttcggcga
      301 gcgggccttg ccgatcctgg tcccgccctg gaatcgcatc gcgccggatc ttgtcagacg
      361 gctgccggag gccggcttcg cgggcctgtc gacctatgcg acgatgatcg ccagcgcgcc
      421 gggccggcgc atcgacaccc acatcgatcc gatcgactgg aaaggcggcg gcgggcgcgt
      481 cggtgcgtcc gcgcacatcg cgaggctggc ggcaatgctt gaggagtccc tcgacaggaa
      541 tccgccggtc cttggaccag tgggcctgtt gacgcatcat ctcgtccatg acccgtgggt
      601 ctgggccctt gtcagggagt tgctgacggt gctgtcggcg catcgcgcgg tgcgctttgt
      661 tgcggcgcgg gaactgtttg gcttgacttg agcggtgcgt cgggcaggga agaggggcgt
      721 ttggcctgat ctcagcacgg gaatcccgtc gagattcggc ccgtgtcgga aatgtctcat
      781 ccagatcaac cggatgaggg attcttgaga cgctccggtg ggtcgtttcg gcgactcccg
      841 gacgaaagcc aggggcaatg cgggtcgggg ctgccgcgtc agggtggtgg gtccgccggg
      901 ggagcgggga tgttggaacg gaccttgtcc gtccgcgatg tggccatcga cttcgtcagc
      961 gatggtcatg tcacgaatgc ggtgaagaac atcagtttcg atctggcgcc cggccggacg
     1021 ctggcgatcg tcggcgaatc cggatcgggc aagtccgtga cgtcgcaggc gatcatgggc
     1081 atcctgtcga agaacggccg ggtgacaaac ggaaccattc atttccgcga tcccaagacc
     1141 ggcgtcatca ccgacatcac cgccctcgac cgcgacggcg aggagatgcg caagctgcgc
     1201 ggcggcaaga tcgccatgat cttccaggag ccgatgacct cgctttctcc gctccacacc
     1261 atcggcgacc agatcggcga ggtggtgaag ctgcatgagg gcgcggacca ggccactgcc
     1321 gaccgccgca ccgccgacct gctcggcaag gtggggttcc gcgacccgga aagggcgctg
     1381 aagatctacc cgttcgaact gtccggcggc atgcgccagc gcgcgatgat cgccatggcg
     1441 ctggtgtgca atcccgccgt tctcgtggcg gacgagccga ccacggcgct cgacgtcacg
     1501 acacaggcgc agattctcga actgatgcgc aagctgcagc gcgagttcaa gatggcggtg
     1561 ctgctgatca cccacgatct cggcgtggtc gccaacatgg cggatgacgt ggtggtgatg
     1621 taccgcggcg aggtgatgga acgcggtccg gttcaggaca tcttccgcga cggccgccac
     1681 gattacctga aggcgctgct gcgttcgatc cccaagttcg gcatgaagga ggacgagcgc
     1741 ctcgtgccga tccgcgccat caagtcgcag atcgccgccg aggaccgtcc gaccggcccg
     1801 cgcgccgccg gatcgggccg caaggtgctg gaggtcaccg gcgtctcgaa gtatttcacg
     1861 ctgcgctctg gcatgttcgg ctcgaagaag acgacgatcc aggcgctttc gaacgtgtcg
     1921 cttgccgtgc cggccggtca gaccctcggg cttgtgggcg aatccggctc gggcaagacg
     1981 accctgtcca agatcatcat gcgcgccatg accccggacg aggggcaaat ccactacgac
     2041 gccgggcagg ggccgaagga tgtggcgaag ctggagggcg cggaactctt cgcctaccgg
     2101 cgcgcggtgc agttcgtgtt ccaggacccg ttttcgagcc tgaacccgcg catgacctgc
     2161 tacgagctcc tctccgagcc gctgcgtatc cacaacatcg gcacgcccga ggaacggttc
     2221 cagcgggtca agtacctgat ggaggtggtt ggcctcgatg tccggcagct gcggcgctat
     2281 ccccatgcgt tttccggcgg ccagcgccaa cgtctcggcc tcgcccgcgc cctggcgctg
     2341 aaaccgtccg tgctgctgtg cgacgagccg accagcgcgc tcgacgtgtc cgtgcaggcg
     2401 cagatcctga acctcctgaa ggacttgcag cgggaactgg acctctcgat cctgttcgtg
     2461 tcgcacaatc tcgccgtggt cgattacatg gcgcaggaga tcgccgtgat gtgccgtggc
     2521 cacatcgtcg aacaggcgcc gcgcgccgcg ctgttcggca acccgcagca tccctacacc
     2581 aaggcgttgc tggcggccgt gccggagccg gacctcgacc gtccgctcga tttcggcaag
     2641 gtctcgggcg gtttttcgaa cccggcggaa tggccggccc cgttcaccat ccgccccggc
     2701 gacttccatc cccgcatggc ggaagtggct ccgggacatt tcgtgcgaat cggcgaggct
     2761 gccagcctcg accgtgcctc gtgaggacag agatgatcgc ttccgcaatg ctgccgtcat
     2821 cacaagggcg cccggcccgg ggcgcgacga agccatccct gctgcgaggg ctccggctgg
     2881 agtgcgttgc cgcagccttc ctcgccatga cggcggtcgc ctccgctgcc gacatccggc
     2941 tcatcgatcc gccgttcttc aaggagcgcg tggaaaaaca gcagctaccg gagatcgccc
     3001 agcgcgtgcc gaagcagccg ctggttgccg atctggaagc gcgcggacgc gtgccgggcc
     3061 gtcacggcgg cgacattcgc acgctggtca gcgccgtgcg cgacctgcgc tatgtcacgg
     3121 tctacggcta cacccgcctc atcggctaca ccgaaaagct cgaactgaag ccggacctgc
     3181 tcgccgacat cgagattgag ggcgggcgcg tgttcaccct gacgctgcgc gagggccaca
     3241 ggtggtccga cggcaagccg ttcaccacgg aggacttccg ctactactgg gaggacatgg
     3301 cgaacgacaa gtcgatcgcg ccggccggcc cgccggaagt ctacttcgtc gatggccagc
     3361 tgccgaagtt cgaggtgctc gacgagcgcc gcgttcgctt cacctggtcg aagccaaacg
     3421 agcgcctgct gccgttctgg gcgcagccgg gcaaggtgtt ctcctatgcc ccggcacatt
     3481 acctgaagca gttccacgcg aaatacacgc ccgaggccga tctgaagaaa cgcatcgccg
     3541 acgccaaggt gcgctcctgg gccgcgctgc acaaccgcat ggacgaccct tacgagaacg
     3601 gcaacgtcga catgccggtg ctgggaccct ggcgcgtgct gacgaagggg ccggccagcc
     3661 gctatgtctt cgagcgcaac ccgtattacc accgcatcga ccgcgagggc cggcaactgc
     3721 cgtatgtcga ccgcatcctc gtcgacatcg cggccggcgg cctaatcgcc gccaaggcca
     3781 acgccggcga gacggacctg cagggcaggg gcctttccat ggtggacgcc ggcgtgctca
     3841 aggaaggcga gaagctcaac ggctaccgca cgctgctctg gccgaacgcg cgcggctcgg
     3901 cctatgcgct ctatcccaac ctcaacaccg ccgatccgga gttcaagaaa ctcaatcgcg
     3961 acctgcgctg gcgccgcgcg ctctcccacg ccatcgaccg gcggatgctc aacaacgcgc
     4021 tgctgttcgg cctcggccgc gaggtcggca acactgtcga gccgcagagc ccgctctatt
     4081 cggaagcggc ggcgaagatg cacatggcct atgatccggc gctggcgaac cgcctgctcg
     4141 acgagatcgg cctgacgcag cgcgactcgc tgggcttccg gctgctgccg gacggtcgcc
     4201 gtctcgagct tctggtggaa ctcaacggcg gcgacggcga catgctggac gcgctccagc
     4261 tcatcgccga gacctgggcc gacatcggca tcaagctgat cgccaagccg caggaccgtt
     4321 caatcctgcg caagcgctcc tacagcgggc agaccgtgat ggtgatgtcc accggcctcg
     4381 acaacgccgt gccgacggcg gacatgccgc cgatcgaact cgcgccggcc gtgcaggaca
     4441 actacgcctg gccgatgtgg ggccagcacg tggagactaa gggcaaatcc ggcgagccgg
     4501 ccgacatccc ggcggcaaag gcgctgctcg acgactacac cgcctggcgc tcgaccaacg
     4561 atccggcgca gaagaccgcg atctggaagc ggatgatcga gaattacgcc gcgaacctgt
     4621 ggaccatcgg cacggtctcg ggcgagttgc agccggtcgt ggtttcgaaa aagctccgca
     4681 acgtgccgga caaggcggtc ttcgcctggg agccgatggc gctgttcggc gtgcatcgca
     4741 tcgacgaatt cttctacgcg gagtgagcgg acgtgcttct ctaccttctg aagcggctgg
     4801 cgacgatgat cgggacgctg tttctcgtct cggcgctcgt cttcggcatc atcaaactgc
     4861 cgccgggcga cttcctgacc aaccagatcg ccgaactcaa ggcgagcggc gatgccgcct
     4921 ccgtcgataa ggtggaattc ctgcgcaagg aatacggcct cgacaagccg gcgtgggagc
     4981 aatacctcgt ctggatcggg ctgatgccgg gcgcgcacgg ctattccggc ctcatccagg
     5041 gtgatctcgg ctggtctttc gaacatcagc agccggtgtc cgccatcctc ggggagtcgc
     5101 tggcgctgac gttgatcgtc aacatcgccg cgatcatctt cattcacgtg ctggccattc
     5161 ccgccgcggt ctattccgcc aagcggcagt attccctgcc ggactacatc atcaccttca
     5221 tcggctatct cggcatcgcc acgccgtcct tcctgctggc gctggtgctg ttgttctatc
     5281 tcaaccgctg gttcggcgtg tcgatcggcg ggctgatgga cccgaagttc gagggccagc
     5341 cctggtcgct ggcgaaggtc ggctccgtgg cggcgcatct catcgtgccg accatcgtca
     5401 tcggccttgc agcgcttgcc tccatggtgc ggcgcatgcg cgccaacctg ctggacgagt
     5461 tgcagaaaca gtatttcgtc acggccaagg ccaagggact cgatccgaac aaggcactga
     5521 tcaagtatcc gttccgcatg tcgctgaacc cgttcatctc cgacatcggc aacatgctgc
     5581 ccggcctcgt ttccggctcg atcctggttt cgctggtgat gagcctgccg acggttgggc
     5641 cgaagctgct gcaagcgctc aaggtctccg accagtatct ggccggctcg atcctgatgt
     5701 tcgtctcgct gctgaccatc atcggcatgt tcatctcgga tctgctgctc gccatgctcg
     5761 atccgcgcat ccgcttcggc gcgaagggga agtgacatga gccagactgt cgcttccgac
     5821 ggcctgccgc cgccgctccc cgcccgcgcc atcgacacca agccgttcaa cccggcagag
     5881 gagcgcatgc ccaccggcga cgaggcgtat taccgcgcct cgacctggac gctgatgtgg
     5941 gcgaagttca agcgcaacaa gctggcggtg atctcgctgt tcgtgctgct ggccttctac
     6001 gggtcgctgg tgatcgtcga gcagatcgcg ccctacgccg ccagcaagcg ccatggcgac
     6061 cacctctacg cgccgcccca gtcggtccac ctgttccacc agggcgagtt cagggggccg
     6121 tttgtctacc cctatgcctt caagttcaat ctggagacgt tcaagcggga gtacgtcatc
     6181 gacgagacca agccgcagcc gatccgcttc ttctgctcgg gcgattccta tgacttcatg
     6241 ggcgtggcga agctggattt ccacttcgtc tgcccgccga aggacggcac gatgttcctg
     6301 ctcggcaccg acaagcttgg ccgcgacctg ctctcgcgca tcatctacgg cgcgcgcatc
     6361 tcgcttaccg tcggcgtcgt cggcatcgcc atttccttca tcatcggcct gacgctcggc
     6421 ggcctcgccg gctatctcgg cggctgggtg gatgcggtgg tgcagcgctc catcgagatc
     6481 ctgcgctcgc tgcccgaact gccgctgtgg ctggcgctct ccgccgccgt gccgccgaac
     6541 tggagtccgg tgacggtgtt cctcgccatc acggtgatcc tcggcttcct cgactggccc
     6601 ggtcttgccc gcgccgtgcg ctcgaagctg ctcgccctgc gcgaggagga tttcgtgcgc
     6661 gccgccgaac tcatgggtgc ctcgaagtcg cgcatcatcg gtcggcatct ggtgcccaac
     6721 ttcatgagcc acctcatcgc ctcggcgacg ctgtcgatcc ctgccatgat cctcggcgag
     6781 acggccctct ccttcctcgg gcttggcctg cgcccgccgg tgacgagctg gggcgtgctc
     6841 ctgaacgacg cgcagaacct cgcctcggtg aacctctacc cctggctgct gacgccgatg
     6901 atcccggtga tcatcgtcat cctcgccttc aatttcgtcg gcgacggcct gcgggatgcg
     6961 gccgacccgt atcactgagg agcgttcgcc gcgcgcgagc gcttgcgacg cgtcaaggcg
     7021 cgaaccgcgc ggttctgcaa tgatgaactc atgcagaatc atccgttttt cgggcgccgc
     7081 gccctgtttg gagcggtcgg tctggtcgcc gcccttctgg ctgcgcccgt cgccgcgcag
     7141 gacccggctt ccgatcccgt tgccgcgctc acgccggcgc aggcctatgc caggggctgc
     7201 ggcagctgtc accgcagcga gcgcggcgtc gtgcgcgcga tcccgaagcg gccggatgcc
     7261 gagcgacggg cctggatcga gaccttcatg gcggatcacc cctgcgagtt tgacgccggc
     7321 aaggcgaaga tcctcgacta cctggtggag aagtcgcggc gctgatcccc gccgacccct
     7381 gatctcgcga ccgtccccac ggcctgcttt tccactatgc cggcgcatgg gatgggtgcc
     7441 gcatgggcat cgcgcgcatc gtcctgcgga cgtctcgccc cgtgaccctt caacggcgat
     7501 gaccgccggc cggcggaaca gggggcttgc gcgcggggca ggagggcatc attaggagtt
     7561 ccgaacgcgc tcgtcgcaag ggcgaggctg atcgcatgcc ggagttccca tgcctgtcga
     7621 atccaagccc ctcgatccga agatcctcgc cgcgctgaag caatgctccg ccgcgaccgt
     7681 caccacgatc ctgctcaaga agggcgtgcg cacggtctgg atgcgtggca cgaagccctt
     7741 gagggccgat cagccgcgca ccgtcggccg ggccttcacg ctgcgcttcg tgccggtgcg
     7801 cgaggacctg gcgacgccgg agtcctggtc caatccgatt tcgacacgcg cggccatcga
     7861 ggcgatgccc gagggctgcc ttgcggtggt ggacgccatg ggcgtcgacg atgccggcat
     7921 cttcggcgac attctctgcg cccgcatggt caagcggaag gtggccggcc tgatcaccga
     7981 tggcgtcgtg cgcgacgcgg aaggcgtgct ctccaccggc ctgccggtct ggtgcaacgg
     8041 cgtcgcggcg cctgcctcgg tcgcgggcct aaccttcgtc ggctggcagg agccgatcgc
     8101 ctgcggcggc gtcgcggtgt tcccgaacga tgtcgtcgtg gtcgacaagg acggcgcggc
     8161 gctgatcccc gaggcgctgc tcgactacgt cgccgagcac ggtccggaac aggagcgcat
     8221 ggaggcctgg atcatgaagg aagtggacaa gggcctgccg ttgcccggcc tctaccccat
     8281 gaacgccgag accaaggccc gctacgaagc ctcgaagaag gcctgagggc acagcgccgg
     8341 cgtctcgcca tgatcaggca ggacggctgg caatttgccc gcgtgacgct atcttgcggt
     8401 catgtcctcg cttgaactga aatatccgcc ggtcgcggtc ttcctggtct gcgccgtgct
     8461 tgtgccggtt ctggcctttg tctttcccgg cttcggcatg agcggcggcg atccctggaa
     8521 atccgcgatc gccagcctcg tcggctcggc cgggctgatg ctgggcctcg ccggtgtcgt
     8581 ccagttccgc atgcaccgca cgaccgtgca tccccagcat ccggagcgct catcgtcgct
     8641 ggtcacggac ggggtctacg gcttcacgcg caacccgatg tatctcgggc tggctctcgt
     8701 gctggcggcc ttcggggtct tctacatgct gcatcttgcc ggcgtgctgt gcaccgcggc
     8761 attcgtgttc tggatgaacc gcttccagat cgagccggag gaacggatga tgcaggagaa
     8821 gttcggcgcc cagtatcacg cctatagcca gcgcgtccgg cgctggattt gaccccggag
     8881 cccgcacctc gccatgaccg cttcctatgc cggcgtcttc ccgatcgccc cgacgccttt
     8941 cctgccggat ggcgccatcg actgggccag cgccgagagg ctgttcgcct ggtatcgcga
     9001 gatcggctcg gatggtctga ccgtgcttgg catcatgggc gaggcgccca agctggaacc
     9061 ggcggaatcg cttcggctgc tgaaactggc ggtaaagcac ttcgccggcc tgccggtgat
     9121 cgtcggggta tccgcgccgg gctttgccgc catgcgctcg ctggcgcgcg aggctctggc
     9181 ggcgggcgcg gcctcggtca tgatcgcgcc gcccggcacg ctgcgcacgg acgacagcat
     9241 cgtcggctac tacgcgcagg cggtggaggc gatcggtgcg gacgtgcctt tcgtcatcca
     9301 ggattacccg ctggtcacct cggtgcagat gacgccgggc gtgatccgcc gcatcgtgca
     9361 ggatcaggcg tcctgcgtca tgctgaaagc cgaggactgg ccggggctgg aaaagatcac
     9421 agccctgcgc tcctggcaga aggatggaac gctgcgcgat ctcgccatca tggtcgccaa
     9481 tggggggctg ttcctcgatt tcgagatgga acgcggcgcc gacgggtcca acaccggtta
     9541 tgcctttccg gaaatgctga tcgacgtcgt gcgcctgtcc aggacaggcc agcgcgatgc
     9601 ggcgcatgac ctgttcgatg cgcatctgcc actgttgcgc tacgagcagc agcccaatgt
     9661 cgggctcgcc gtgcgaaagt acgtcatgat gcgccggggc atcctggcgc acgatgcgca
     9721 gcgcaagcct ggcgccgggc tgagcgcgac ggcacgcgcg gaagtggact acctgctcgc
     9781 ccgccttgcc cgcgtggatc gccgcgcggc ggtctgatac cgctcgcagg tgcatgtcag
     9841 atcgagagcg gacctcgcat tcggcgcggg ccggtgcgct gttcgcaatg tgcgctagcg
     9901 cacatcgtct atcgcgttcc cgtatacctt ggct
//
LOCUS       DAOMXN010000081         9863 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_3630_length_9863_cov_10.551998, whole genome shotgun sequence.
ACCESSION   DAOMXN010000081 DAOMXN010000000
VERSION     DAOMXN010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 9863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9863
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3630_length_9863_cov_10.551998"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /locus_tag="PK812_07400"
     CDS             complement(<1..974)
                     /locus_tag="PK812_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRJ69419.1"
                     /translation="MPIHSPSSEAEAAEIVSAARAARTALRIVGGGTRQDLGRPVNAT
                     ARLSTEKLGGITLYEPAEMVIGARAGTPLDEVVATLAAKGQMLPFEPMDHRPLLGSTG
                     APTMGAIAAGNISGPRRISHGAARDSLIGVRFVNGRGEIVKSGGRVMKNVTGLDVVKL
                     QSGAWGTLGFLTEVIFKVLPAPETQRTLVLEGLDDARGIAALSAGLTSPFEVTGAAHL
                     PATDTAAARTMLRIEGFEASLTYRAAALGRLLTPFGAARILEESASASLWRMVRDAEP
                     LLEPRDAAVWRLSVKPSDGPKVHETVAHRIAGHIQIVRPRYLDSGIADP"
     gene            complement(977..2407)
                     /locus_tag="PK812_07405"
     CDS             complement(977..2407)
                     /locus_tag="PK812_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRJ69420.1"
                     /translation="MNAIAFPPPSAAVLARRDAIIAGLAALVPDDALITSNNECKAFE
                     TDAFTAYRRMPLAVVLPRTTDEVSAILAFLNREGVPVVPRGAGTSLCGGAIPQEDAVV
                     LGVGKMSRILEVDTANRFARVQAGVTNLAISAAVDGAGFFYAPDPSSQLACTIAGNIA
                     MNSGGAHCLKYGVTTNNILGVRAVLLDGTVVDIGGPHVDAPGLDVLGLLCGSEGQLAV
                     ITEATVRILPKAEGARPVLFGFDTSEAAGAAVAAIIGAGIVPVAMEFMDRPAIEICEA
                     FAKAGYPLDVEAMLIIEVEGSDEEMAASLARIVEIAKAHGVRTVKESRSATETAQIWK
                     GRKSAFGATGRIADYICMDGTIPTGQLPYVLRRIDEIVKHYGLRVANVFHAGDGNLHP
                     LILYNVNDPAEAMKAEDAGNDILKLCVEVGGCLTGEHGVGIEKRDLMRHQFNDADLAQ
                     QMRLRAVFDPAWLMNPAKVFPLEGRI"
     gene            complement(2482..3783)
                     /locus_tag="PK812_07410"
     CDS             complement(2482..3783)
                     /locus_tag="PK812_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3422 domain-containing protein"
                     /protein_id="HRJ69421.1"
                     /translation="MNDTANTGIGSRLEPHPLRSVVLGEMHARPFHPVTPPGRMLRLV
                     FMTDEAQAAGSRAALQSLCATLGGAGPGPGEKHCRVALPRGLLRWEQHNEFTTYTFEP
                     KDASATPFEPAATELCDAMRVLPQPGPLLASIDLHFLAQRPSAGYEAWLDPMSLAASS
                     LIAGKAEAATDFRPTGDGFVRLMVIADDLQPNSAGAMTQRLLEVETYRVLALLGLPEA
                     QRLAPVVKGIEDQLTAIARRMTEVEGLEANHQLLRQLTTLAAQLEAEATTAGFRFSAS
                     RAYDGIVQQRLESLKEGPLEGHSMFSSFLARRMGPAMRTCRMMEERQSALATRLSRTA
                     DLLRTRVQLDMETQNRDLMASMNERTRLQLRMQQTVEGLSVAAVSYYVLGLIGYLFKG
                     AKDGGVLPFDPAIATAAVLPAIVLLVALAVRRIRRKHAEDH"
     gene            3845..6385
                     /locus_tag="PK812_07415"
     CDS             3845..6385
                     /locus_tag="PK812_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response DEXH box
                     helicase"
                     /protein_id="HRJ69422.1"
                     /translation="MCVIPVDSFPDESLPEPFAAWFAARGWSVRPHQSTLLQKARLNR
                     SALLIAPTGAGKTLAGFLPSLVDLAGGRRRHAGVHTLYVSPLKALAVDIARNLEVPVA
                     EMGLAVRIETRTGDTPASRRQRQRRDPPDILLTTPEQVALLIASADAPHLFGAVKRII
                     IDEAHALAPIKRGDLLALGLERLKRIAPGVTLTGLSATVRDPDDLARWLGGATTPADC
                     VIADRKSAAAPDIRILETDERLPWAGHTARHAMDAIYRTLKRSKLSLIFVNTRMQAEF
                     VFQLLWAINDDNLRIALHHGSLDVGQRRRVEAAMAAGSLDAVVCTSTLDLGIDWGDVD
                     LVIHVGAPKGASRLLQRIGRSNHRLDEPSRALLVPANRFEVLECRAAIDAANAGAQDA
                     EVIRAGGLDVLAQHVLGMAVAAPFDADALYAEVTCAAPYATLARGDFDAIVDYVATGG
                     YALKSYERFARIRRTKDALWRVSNPAIAQGYRMNVGTIVEEPMLKVRLGRARSTGLGP
                     TGPISRGGRVLGEVEEYFVEQLTYGDTFVFAGEVLRFEAIVENEAVVSRASASDPKVP
                     SYMGGKFPLSTHLAERVRAMLADRTAWSGLPEQVAAWLALQADASLLPARADMLVETF
                     PRGARHYLVAYPFEGRLAHQTLGMLLTRRLERARLKPLGFVASDYAIAVWGAEDMSRA
                     AWREPAFIGDLFSQDMLGDDLEEWLAESSLMKRTFRKAAVIAGMIERRHPGMEKKTRQ
                     VSMSSDLIYDVLRRHQPDHILLKAARADAATGLLDLARLAQMLSRIRGRIIHKPLDRV
                     SPLAVPVMLEIGREAVYGEAADALLEEAADDLVAEAMGHDFGRAGHRD"
     gene            6354..7058
                     /gene="pdeM"
                     /locus_tag="PK812_07420"
     CDS             6354..7058
                     /gene="pdeM"
                     /locus_tag="PK812_07420"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     endonuclease PdeM"
                     /protein_id="HRJ69423.1"
                     /translation="MISAAQATGTETMPVAITIGGERFFADVTGALWHPGHQALLVAD
                     LHLEKGSSYARSGQLVPPFDTIATLSRLAAAATRWRARSIFVLGDAFHDAFAEERIAD
                     EAVAAIDALAAGRRLVWIKGNHDPAPPEQVPGDRADEVRLGDLVLRHEPRKALEGAEI
                     AGHLHPVARVATRAQVVRRRCFVHDERRLLLPAFGAYAGGISVRLPVIAELFSMPSVH
                     VLGERRVLSIARDRIV"
     gene            7246..9660
                     /gene="pheT"
                     /locus_tag="PK812_07425"
     CDS             7246..9660
                     /gene="pheT"
                     /locus_tag="PK812_07425"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRJ69424.1"
                     /translation="MKLTLSWLKDHLDTNASLEVIVETLTRIGLEVEGVEDPARKLAG
                     FSIGYVVEASQHPNADRLRVCLVDTGQGAPVQVVCGAPNARTGMKGVFSPVGTYIPGK
                     DLVLGKGVIRGVESNGMLCSEAELLLSQDHDGIIDLPADAPVGTPFAEYLGLNDPVIE
                     INLTPNRPDATGIAGIARDLAAAGLGTVKTPEPKALTGGDPCPVKVKLDFAAGDAHLC
                     PAFALRLVRGVKNGPSPGWMQARLKAIGLRPINALVDITNYMTFDRGRPLHVFDLKKV
                     KGDLVVRRARKGEEVLALDGKTYALEESMVVIADANGVESIGGIMGGEHSGCDETTTD
                     VLIESALWDPMNIARTGRALGINTDARYRFERGVDPAFCVPGLDMATQLVVDLCGGTP
                     GAMELSGAVPQPDRIIDFPWSETQRLTGLELPRAEAKVILQSLGFWVSGNGDAVKVAV
                     PSWRPDVEGKADIVEEITRIAGVDSVPMVPMRRIDQVHPPVLTLIQRRTRQAKRLLAS
                     RGMLEAVTWSFISKSAADLFGGGQAELALANPIASDLSDMRPTLLAGLLSAAQRNADR
                     GFGNVALFEVGQIFRGDKPEDQKIAASGIRRGLAGPAGLGRHWSGAKSADVFDVKADA
                     LAVLSMLGAPMAGVQITPKAPDWFHPGRSATLHMGKDVFGWFGEIHPRALEALDVEGP
                     IHAFEIVLDALPAPKARPTKMKPKLDVADLQPVTRDFAFLVSSETRAEDLLKAARTVD
                     KALTASVTLFDLYEGKGIDPGKKSVAIEVCLQPRDKTLTDAEIEAFSAKLVAEVAKKT
                     GAVLRG"
BASE COUNT         1555 a         3271 c         3394 g         1643 t
ORIGIN      
        1 gggtcggcga ttcccgaatc gaggtagcgc ggtctgacga tctggatgtg gccggcgatg
       61 cggtgcgcca ccgtctcgtg caccttcggg ccatcggacg gtttcacgga caggcgccag
      121 acggcggcgt cgcgcggttc gagcagcggc tcggcgtcac gcaccatgcg ccagagcgaa
      181 gcggaggcgg attcctccag aatgcgcgcc gcgccgaacg gcgtaagcag gcggccaagc
      241 gctgccgccc gataggtgag cgaggcctcg aagccttcga tgcgcagcat cgtacgcgcc
      301 gcagccgtgt ccgtcgccgg caggtgagcg gcgccggtga cctcgaaggg cgaggtgagg
      361 ccggccgaca gggcggcgat gccgcgcgca tcatccaacc cttccagaac aagtgtgcgt
      421 tgcgtctcgg gcgccggtag caccttgaag atgacctcgg tcaggaagcc aagcgtgccc
      481 caggcgccgg actgcaattt gacgacgtcg aggccggtga cgttcttcat cacccggcca
      541 ccggacttca cgatctcgcc gcgcccgttg acgaagcgca cgccgatcag gctgtcgcgc
      601 gccgcgccgt gagagatgcg gcgcggaccg gaaatgttgc cggccgcgat cgcgcccatc
      661 gtcggcgcgc cggtcgaccc cagcagcggg cgatgatcca tcggctcgaa cggcagcatc
      721 tgacccttcg ccgcgagcgt cgcgacaacc tcatccagcg gagtgccggc gcgcgcgccg
      781 atcaccattt cggccggttc gtagagcgtg atgccgccga gcttctcggt ggaaagcctg
      841 gccgtcgcgt tgaccggacg accaaggtcc tggcgcgtgc caccgccgac gatgcgcaac
      901 gccgttcggg ccgcgcgcgc ggcggaaacg atctcggcgg cttcggcctc cgaggacgga
      961 gaatgaatgg gcagggtcaa atccggccct ccagcgggaa gaccttggcc ggattcatca
     1021 gccaggccgg atcgaaaacc gcgcgcaggc gcatctgctg cgccaggtcc gcgtcgttga
     1081 actgatggcg catcaggtcg cgcttctcga tgccgacgcc gtgctcgccg gtcagacagc
     1141 cgccgacctc gacgcagagc ttcaggatgt cgttgccggc gtcttcggcc ttcatcgcct
     1201 cggcgggatc gttgacgttg tagaggatca gcgggtggag gttgccgtcg ccggcatgga
     1261 acacattggc gacccgcagg ccataatgct tcacgatctc gtcgatgcgg cgcaggacgt
     1321 agggcaactg tccggtcggg atggtgccgt ccatgcagat gtagtcggcg atgcggccgg
     1381 tcgcgccgaa ggccgatttc cgccccttcc agatttgcgc ggtctcggtg gccgagcggc
     1441 tttccttgac ggtcctgaca ccatgcgcct tggcgatctc gacgatgcgg gcaaggctcg
     1501 ccgccatctc ctcatctgac ccctcgacct cgatgatcag catggcctcg acatcgagcg
     1561 gatagccggc cttggcgaaa gcctcgcaga tctcgatggc gggcctgtcc atgaactcca
     1621 tggcgaccgg cacgatgccc gcgccgatga tggcggcaac ggcagcgccg gccgcctcgc
     1681 tggtgtcgaa gccgaacagc accggccgcg cgccttccgc cttcggcagg atgcgcaccg
     1741 tcgcttcggt gatgacggcg agttggcctt ccgagccgca gagcaggccg aggacatcga
     1801 ggccgggcgc atccacatgc ggcccgccaa tgtcgacgac ggttccgtcg agcagcaccg
     1861 cgcgcacgcc gagaatgttg ttggtcgtga cgccatactt caggcaatgc gcgccgccgg
     1921 aattcatcgc gatgttgccg gcgatggtgc aggccagctg cgaggacggg tccggcgcat
     1981 agaagaagcc ggccccgtcc acggcggcgg aaatggccag gttggtcacg cccgcctgca
     2041 cgcgtgcaaa ccggttggcc gtatccacct ccagaatccg tgacatcttg ccgacgccga
     2101 gcacgacggc atcttcctga gggatggcgc ccccgcacag cgaggtgccg gcgccgcgcg
     2161 ggacgacggg aaccccctcg cgattcagga aggccaggat ggcgctgacc tcgtcggtgg
     2221 tgcgcggcag gaccacggcg agcggcatgc ggcgataggc ggtgaaggca tcggtctcga
     2281 aggccttgca ctcgttgttc gaggtgatca gggcgtcgtc aggcaccagg gcggcaagac
     2341 ccgcgattat cgcgtcacga cgggccagaa cggcggcgct tggcggcggg aatgctatgg
     2401 cgttcatggc gtctccccgg ggcggttcat gcgggtgcct gaaccatagc ggctttgacg
     2461 ccggcaagcc aagcgcggct ctcaatggtc ctctgcatgc ttgcggcgga tgcggcgcac
     2521 cgcgagcgcg accagcagga caatggcggg aagaaccgcg gcggtcgcga tggccgggtc
     2581 gaaaggcagc acgccgccat ccttcgcgcc cttgaacagg taaccgatga gaccgagcac
     2641 gtagtagctg accgcggcga cggaaagtcc ctcgaccgtc tgctgcatgc gcagttgcag
     2701 gcgcgtgcgc tcgttcatcg acgccatcag gtcgcggttc tgcgtctcca tgtcgagttg
     2761 aacgcgggtg cgcagcaggt ccgccgtgcg cgacaggcgg gttgcgagtg cgctctgccg
     2821 ctcctccatc atgcgacagg tgcgcattgc cggccccatg cgcctggcca ggaaggagga
     2881 gaacatggaa tggccctcca gcgggccttc cttcagcgat tcgaggcgtt gctgaacgat
     2941 gccgtcgtag gcgcggctgg cgctgaaacg gaaaccagcg gtcgtggctt ccgcctccag
     3001 ttgggcggca agcgtcgtca gctggcgcag gagctggtgg ttcgcctcca ggccctccac
     3061 ttccgtcatg cgtcgcgcga tggcggtcag ctggtcctcg atgcccttga cgaccggcgc
     3121 gagccgctgc gcctccggca ggccgagcag cgccagaacc ctgtaggtct cgacttcaag
     3181 caacctctgc gtcatcgcgc cggcagaatt cggctgcagg tcatccgcga tcaccatgag
     3241 gcgcacgaac ccatcgccgg tgggccggaa gtcggttgcg gcctctgcct tgccggcgat
     3301 gagcgacgaa gcagccaggc tcatcgggtc gagccatgcc tcgtagccgg ccgagggccg
     3361 ctgcgcgagg aaatgcaggt cgatcgacgc cagcagcgga ccgggctgcg gcaggacacg
     3421 catcgcgtcg caaagttcag tggcggcagg ctcgaaaggc gttgccgacg cgtccttcgg
     3481 ctcgaatgtg taggtcgtga actcgttgtg ctgttcccag cgcaggagac cgcggggcag
     3541 cgccacgcgg caatgctttt cgccaggacc gggaccggct ccgccaaggg tcgcgcacag
     3601 cgactgcagg gccgctcgac tgcccgccgc ctgcgcctcg tcggtcatga acaccaggcg
     3661 cagcatgcgg ccgggcggcg tgacggggtg gaaggggcgg gcatgcatct cgccaagcac
     3721 gacgctccgc agggggtgcg gttcgagcct gctgccaatt ccggtgttcg ccgtgtcgtt
     3781 cacgcctgcc gccttcgcgc atcgcgccac cgcgctcgac ggcggcttcg gctgtgcttt
     3841 actgatgtgc gtgatccctg tcgattcctt cccggacgaa tccctgccgg agccgtttgc
     3901 ggcgtggttt gccgcgcgcg gctggagcgt ccggccgcat cagtcgactt tgctgcagaa
     3961 ggcgcgcctc aatcgctccg ccttgctcat cgcgccgacc ggggcgggca agaccctggc
     4021 cggcttcctg ccgagcctcg tcgaccttgc gggtggccgg cggcgtcatg ccggtgttca
     4081 caccctgtac gtctcgccgc tgaaggccct cgccgttgat atcgcccgca atctggaagt
     4141 gcctgtcgcc gagatggggc tggcggtcag gatcgagacg cgcaccggcg acacgccggc
     4201 atcccgccgc cagcgccagc gccgcgatcc gccggacatc ctgctcacga cgccggaaca
     4261 ggtggcgctg ctcatcgcca gcgcggatgc gccgcatctg ttcggtgccg tgaagcggat
     4321 catcatcgat gaagcgcatg cgctcgcgcc gatcaagcgt ggggacctgc tggcgctggg
     4381 tctggaacgt ctgaagcgga tagcccccgg cgtgacgctg accgggctgt ccgccacggt
     4441 gcgcgacccg gacgacctgg cccgctggct cggcggtgca acaactccgg cggactgcgt
     4501 gatcgctgac cggaaatcgg ctgcggcgcc ggacatccgc atcctcgaaa cggacgagcg
     4561 gttgccctgg gcagggcaca ccgcccgcca cgcgatggac gcgatctacc ggacgctgaa
     4621 gcgcagcaaa ctgtcgctca tcttcgtcaa tacgcggatg caggcggagt tcgtgttcca
     4681 gctgctgtgg gcgatcaatg acgacaatct gcgcatcgcc ctgcatcatg gctcgctcga
     4741 cgtcggccag cgccggcgcg tcgaagccgc gatggcggca ggctccctgg atgccgttgt
     4801 ctgcacctcc acgctcgatc tcgggatcga ctggggcgat gtcgatctgg tcatccatgt
     4861 cggagcgccg aagggcgcga gccgcctgct gcaacgaatc ggccgctcga accaccgtct
     4921 ggatgaaccc tcgcgcgccc tgctcgtgcc ggcgaaccgc ttcgaggttc tggagtgccg
     4981 cgccgccatc gacgccgcca acgccggggc gcaggatgcc gaggtcattc gcgccggcgg
     5041 gctcgatgtg ctcgcgcagc atgtgcttgg catggcggtc gcagccccct tcgacgccga
     5101 tgcgctctat gccgaggtca cttgcgccgc tccctacgca accctggcgc gcggggattt
     5161 cgacgccatt gtcgattatg tcgcgaccgg cggctatgcg ctgaaatcct atgagcgctt
     5221 tgcccggatc aggcggacga aggacgcgct ctggcgggtt tccaatcccg ccatcgccca
     5281 gggttaccgc atgaatgtcg gcaccatcgt cgaggaaccg atgttgaagg tgcggcttgg
     5341 ccgcgcccgc tcgaccgggt tggggccgac cggtccgatc agccgcggcg gacgcgtgct
     5401 cggggaagtc gaggaatact tcgttgagca actcacctac ggcgatacct tcgtcttcgc
     5461 cggtgaagtg ctgcgcttcg aggcgatcgt cgagaacgag gcggtggtct cgcgggcgag
     5521 cgccagcgat cccaaggttc cctcctacat gggcggcaag tttccgctgt cgacccatct
     5581 cgcggagcgg gtccgggcca tgctggcgga ccggacagcc tggagcggcc tgcccgaaca
     5641 ggtggccgca tggctcgccc tccaggcgga tgcgtccctg ctgcccgcgc gcgcggacat
     5701 gctggtggaa accttcccgc gcggcgcgcg gcactatctc gtggcctatc ctttcgaagg
     5761 gcggctcgcc caccagacgc tcggcatgct gctcacacgc cgtctcgagc gggcgcgact
     5821 gaagccactc ggcttcgtgg cctcggatta cgccatcgct gtctggggcg ccgaggacat
     5881 gtcgcgggcg gcctggcggg agccggcgtt catcggcgac ctgttctcgc aggacatgct
     5941 gggcgacgac ctggaggagt ggctggcgga atcctccctg atgaagcgga ccttccgcaa
     6001 agcggccgtc atcgccggca tgatcgagcg tcgccatcct ggcatggaaa agaagacgcg
     6061 gcaggtgagc atgtcgagcg atctcatcta tgacgtgctg cgccggcatc agccggacca
     6121 tatcctgctc aaggcggcgc gggcggatgc cgccaccggc ctgctcgatc tcgcgcgcct
     6181 ggcgcaaatg ctctcgcgca tccgggggcg aatcatccac aagccattgg accgcgtttc
     6241 gccgctggcc gtgccggtga tgctggagat cggccgcgag gcggtctacg gcgaagccgc
     6301 agacgcgctc ctggaagagg cggcggacga tctggtcgcg gaggcgatgg ggcatgattt
     6361 cggccgcgca ggccaccggg actgagacca tgccggtggc gatcacgatc ggcggcgagc
     6421 gctttttcgc ggatgtgacc ggcgcgctct ggcatcccgg gcatcaggcg ttgctggtgg
     6481 ctgacctgca tctggaaaag ggctcgtcct atgcccggtc gggacagctt gtcccgccct
     6541 tcgacacgat cgccaccctg tcccggctgg ctgcggccgc cacgcgctgg cgtgcccgat
     6601 cgatcttcgt gctgggcgat gcgtttcatg acgccttcgc cgaggagagg atcgcggacg
     6661 aggccgtcgc ggccatcgat gcgctcgcgg ccggccgtcg actcgtgtgg atcaagggca
     6721 atcacgatcc cgcgccaccg gagcaggtgc cgggggatcg ggcggacgag gttcggctag
     6781 gtgacctcgt cctgcgccat gaaccccgca aggcgctgga gggcgcagag atcgcggggc
     6841 atctgcatcc ggtcgcccgg gtcgcgacgc gcgctcaggt cgttcgccgc cgctgtttcg
     6901 tccatgatga gcgtcggctg cttcttcccg ccttcggagc ctacgccggc gggatcagcg
     6961 tgcgccttcc ggttatcgcc gagctgtttt caatgccatc cgtgcatgtg ctcggcgagc
     7021 gtcgcgtgct gtcgatcgcg cgcgatcgga tcgtctgagc ccgaatggga gcgctttggc
     7081 ctgcttgccg gcccagagcg cagttgcgct tcctgaaatc gcggctattg aacggcgctg
     7141 aaagctttct cctcaccagg aaggcctgct ttgatcgcgc gtcttatcgc cggtctccct
     7201 tgcgggagca tggcgctcaa ggccagttga agcgggacag gatcgatgaa acttaccctt
     7261 tcgtggctca aagaccatct cgacaccaac gcctcgctgg aggtgattgt cgaaacgttg
     7321 acccgcattg gccttgaggt cgaaggcgtc gaggacccgg cccgcaagct ggccggcttt
     7381 tccatcggct atgtcgtcga ggcaagccag cacccgaatg ccgaccgctt gcgcgtctgt
     7441 ctcgtcgaca ccgggcaggg cgcgccggtt caggtggttt gcggtgcgcc gaatgcaaga
     7501 accggcatga agggcgtgtt ctcgccggtg ggaacctaca tccccggcaa ggacctggtg
     7561 ctcggcaagg gcgtgatccg cggcgtcgaa tccaacggca tgctctgctc ggaagccgaa
     7621 ttgctgctgt cgcaggatca tgacggcatc atcgatctgc cggccgatgc gccggtcggc
     7681 acgccattcg ccgaatacct tggtctcaac gaccccgtca tcgagatcaa cctgacgccg
     7741 aatcgtccgg atgcgaccgg cattgccggc atcgcgcggg atctcgccgc cgcggggcta
     7801 ggcacggtga agacgccgga gccgaaggcg ttgaccggcg gcgatccctg tccggtcaag
     7861 gtgaagctcg atttcgccgc tggcgatgcg catctctgcc cggccttcgc cctgcgcctg
     7921 gtgcgcggcg tgaagaatgg cccgtcgccg ggctggatgc aggcccggct gaaggctatc
     7981 ggtctgcggc cgatcaacgc gctggtcgac atcaccaact acatgacctt cgatcgcggc
     8041 cgtccgctgc acgtcttcga cctgaagaag gtcaagggcg atctcgtcgt gcgcagggcg
     8101 cgcaagggcg aggaggttct ggcgctcgac ggcaagacct atgcgcttga ggagtcgatg
     8161 gtcgtcatcg cggacgccaa cggcgtcgaa tccatcggcg gcatcatggg cggcgagcat
     8221 tccggctgcg atgagaccac gaccgacgtg ctcatcgaat ccgcgctctg ggacccgatg
     8281 aacatcgccc gcaccggtcg cgcgctgggc atcaacaccg acgcgcgcta ccgcttcgag
     8341 cgcggtgtcg atccggcctt ctgcgtgccc ggcctcgaca tggcgacgca gctggttgtc
     8401 gacctgtgcg ggggaacgcc cggcgccatg gaactgtccg gcgccgtgcc gcagcccgac
     8461 aggatcatcg acttcccctg gtccgagacg cagcggctga ccgggctgga actgccgcgc
     8521 gccgaagcca aggtgatcct gcaaagcctg ggcttctggg tctccggcaa cggcgacgcg
     8581 gtgaaggtcg cggtgccctc ctggcggccc gatgtcgagg gcaaggcgga catcgtcgag
     8641 gaaatcaccc gcatcgccgg cgtggactcc gtgccgatgg tgccgatgcg ccgcatcgat
     8701 caggtgcacc ccccggtgct gacgctgatc cagcgccgca cgcggcaggc caagcgcctg
     8761 ctcgcctccc gcggcatgct ggaagcggta acctggtcgt tcatctcgaa gtcggcggcg
     8821 gacctgtttg gaggcggtca ggcagaactg gcgctggcca atcccatcgc ctccgacctg
     8881 tccgacatgc ggccgaccct gctggctggc ctgctgtccg ccgcgcaacg caatgcggac
     8941 cggggtttcg gcaacgtcgc cttgttcgag gttggccaga tcttccgggg cgacaagccg
     9001 gaggaccaga agatcgccgc ctccggcatc cggcgcggcc ttgccgggcc ggcgggcctc
     9061 ggccgtcact ggagcggcgc gaagtctgcg gacgtgttcg atgtcaaggc ggatgcgctg
     9121 gccgtgctct cgatgctcgg cgcaccgatg gccggcgtcc agatcacgcc caaggcgccg
     9181 gactggttcc accccggacg atccgccacc ctgcacatgg gcaaggatgt gttcggctgg
     9241 tttggggaaa tccatccccg ggccctggaa gcgctggatg tcgaaggccc gatccatgcc
     9301 ttcgaaatcg tgctcgatgc gttgccggcg ccgaaggcgc ggccgaccaa gatgaagccg
     9361 aagctcgatg tcgccgatct ccagcctgtg acgcgcgact tcgccttcct ggtgtcgagc
     9421 gaaacgcgcg ccgaggacct gctgaaggca gcgcgcaccg tcgacaaggc gctgaccgcc
     9481 agtgtcacgc tgttcgatct ttatgaaggc aagggcatcg atccgggcaa gaagtcggtg
     9541 gccatcgagg tgtgcctgca accgcgcgac aagaccctga cggacgccga gatcgaggcg
     9601 ttctcggcaa aactcgtggc ggaggtcgcg aagaagaccg gggctgtcct gcgcgggtag
     9661 gcccggattc tcgccgcaaa gctggagcat gatccgcgca tgatccgcat tgtcatgctg
     9721 gccgttcttc tggtcgccgg gagcagcctc ccggcggcgg cgaggacgcg cgccgattgc
     9781 gacaggatcg agaacccgat gctgttcaac gaatgcctcg cctcgctggc gccggagcgt
     9841 cagccgcgcg cgcggcgggt ggg
//
LOCUS       DAOMXN010000082         9661 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_3731_length_9661_cov_12.293462, whole genome shotgun sequence.
ACCESSION   DAOMXN010000082 DAOMXN010000000
VERSION     DAOMXN010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 9661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9661
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3731_length_9661_cov_12.293462"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PK812_07430"
     CDS             <1..186
                     /locus_tag="PK812_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HRJ69425.1"
                     /translation="SPIFGMPILDVERAKTVFFIKRGMSSGYAGVDNELFFKPNTMML
                     FGDAKKMTEEIVKAMAH"
     gene            290..1114
                     /locus_tag="PK812_07435"
     CDS             290..1114
                     /locus_tag="PK812_07435"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRJ69426.1"
                     /translation="MKKRILPALIGLLLVVYIAIAAYMGAFQRSFIYAVAPQKPAVAA
                     ASVPGLTETDLVMADGVRLNTWIVPPTAGDRPIYVYFHGNANTLMRRAERFRLLTSNG
                     SGLLAIHYRGYGGSGGKPTEALLHKDAEAAFAEAEKRFPGRKLVAFGESLGSSIAIRL
                     ASERPQVAAVVLDCPFVSVLSRAQASYPWLPVSLLLVDTFRSDRWIGTVKAPIFIMHG
                     ALDRVTPIEEGRKLFALAPDRKRFVAYPDAGHVDNFRFGAADAIWQFLKDMAVIPG"
     gene            complement(1189..1527)
                     /locus_tag="PK812_07440"
     CDS             complement(1189..1527)
                     /locus_tag="PK812_07440"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRJ69427.1"
                     /translation="MLEVERAVIGYLAYFWPKSAPYLPKSDRPILHIENIVVAGSNRR
                     RGYGRMLLAHADAVALAGNARAITLAAVVNNALALGAYRAAGYSPVSYEMVKPLDDSM
                     RGLGYQPASG"
     gene            complement(1704..2576)
                     /locus_tag="PK812_07445"
     CDS             complement(1704..2576)
                     /locus_tag="PK812_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012453827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ69428.1"
                     /translation="MLIRMIPVDHFRLAHVLGTVALALSLSACETTMGGLNPPGVAEI
                     DDANTAGASSANISSLSEVIARNPNDPQAYNTRGAAYARASRFNDAIADFSKAIQLDP
                     NFAPAFVNRGLAYRQANKNDLAFADFNRAIQADPNYAQAYLARGNLYRAQNNLDAAMN
                     DLTHVIRIKPDAAEAYHGRGLVYQRRGQHALAMSDFDAAVDRNPYVAPPYVARGQSLN
                     ATGRFDQAAEDFTAALNVDNRNADAWAGRGYALDQQGKRAEANEAYQRALSIDPNHRG
                     ARESMSRQRSGVFR"
     gene            complement(2720..2956)
                     /gene="rpsU"
                     /locus_tag="PK812_07450"
     CDS             complement(2720..2956)
                     /gene="rpsU"
                     /locus_tag="PK812_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HRJ69429.1"
                     /translation="MQVLVRDNNVDQALRALKKKMQREGIFREMKLRGHYEKPSEKRA
                     REKAEAVRRARKLARKRLQREGLIAAPKPKVKKP"
     gene            complement(3169..4386)
                     /locus_tag="PK812_07455"
     CDS             complement(3169..4386)
                     /locus_tag="PK812_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HRJ69430.1"
                     /translation="MRRDLMRYSGTVARPMFRLLLVLLCLGGGLAPARADFAAFLVGL
                     WPEAQAAGVSRATFDGAFKGVTPDQKIVAQTKKQSEFVRPIWDYVEGAITSGRVERGR
                     EAGGRWSDTVSAVESRYGVDRRAVMGIWGMETNFGSFFGNNSVIRALATLAYVRYRDD
                     FFRKELIIALKILEEGHVERSEMRGSWAGAMGHTQFMPSSFMKYAVDHNRDGRKDIWD
                     SIPDALASTANYLKEHGWQTGLPWGMEVELPHGIDLRQALGRKAFPAFSALGIKRASG
                     QALPRRGEAALFLPAGIKGPAFLLTDNFKVIKAYNSSDAYALAVGLLGDVIYGGEPVQ
                     ADWPKKDKRLTRDQGMEVQRQLTKRGLYQGKIDGRFGEQSRESIIRFQKQAGLVPDGY
                     ATPALLERLKLAR"
     gene            complement(4376..5365)
                     /gene="galE"
                     /locus_tag="PK812_07460"
     CDS             complement(4376..5365)
                     /gene="galE"
                     /locus_tag="PK812_07460"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009492967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HRJ69431.1"
                     /translation="MSVLVTGGAGYIGSHMVHALRDTGQKVVVLDNLSTGFAWAVPPD
                     VPFFGGDAGDQTLVKRIVAEHGIESICHFAAKIVVPDSVVDPLGYYLANTVTSRSLIE
                     AAVQSGVKRFVFSSTAAVYGEPKVMPVSENTPLAPMSPYGTSKMMTEFMLRDTANAHD
                     FRYVALRYFNVAGADPYGRTGQSTPNATHLIKVAVQTALGHRSHLDLYGTDYDTPDGS
                     CVRDYIQVSDLAAAHIKALDYLGRGGRSTVYNCGYGTGYSVLQVIEVVKQVSGVDFEV
                     RAMPRRAGDSPAVVAKADRIRAELGWKPEHDDLRRIVEQALRWERHLKARNAA"
     gene            5554..6426
                     /locus_tag="PK812_07465"
     CDS             5554..6426
                     /locus_tag="PK812_07465"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017486516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HRJ69432.1"
                     /translation="MQPKKIRKAVFPVAGLGTRFLPATKSVPKEMMNVVDRPVLQWVV
                     DEAREAGIEHLIFVTGRNKAVIEDHFDIAYELEATLKERGKLKELEALQKDLPPAGMA
                     SFTRQQAPLGLGHAVWCARDLVGDEPFAVMLPDMLSRGCMKQMVDAYEKHGGNVIALE
                     QVPMDQTHQYGIVAVGQAHGRVHELTGMVEKPKPGTAPSNYIISGRYILQPEVFSILS
                     AKEKGAGGEIQLTDAMIKLMGKQRFFGMHYDGRTYDTGSKIGFLAANVAYGLARAELK
                     DEFRAEIERILAGK"
     gene            complement(6463..6867)
                     /locus_tag="PK812_07470"
     CDS             complement(6463..6867)
                     /locus_tag="PK812_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69433.1"
                     /translation="MNEKVDRDSLTGIWHGQYSYPAPQVPPVAFVATLIQFGEMLSGT
                     THENIETDMGDAALFATLEGTRQGEAVNFVKTYDPAIEDWQDVIYDGRLNGAGDEIEG
                     TWTIPGVLAGKFLMIRNRGEARARAKRKRAEV"
     gene            complement(6875..8290)
                     /locus_tag="PK812_07475"
     CDS             complement(6875..8290)
                     /locus_tag="PK812_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HRJ69434.1"
                     /translation="MDNRSSVWRQVDQARERFVALADRVWGMPEVCYTEVRSVAEHIE
                     QLSVEGFRITRNVAGIPTAVIGEAGEGGPVIAFLGEYDALPGLSQEAGVAEHRPIEAG
                     GHGHGCGHNLLGSAAMLAATALKNWLAENKLPGRVRYYGCPAEEGGAAKAFMVRAGVF
                     KDADIAITWHPYSFWEVTPPLSLANTRIDFTFTGRAAHAAASPELGRSALDAVELMSV
                     GVNYMREHMPSDARVHSAILDTGGIAPNVVQASAKVRYLIRARDLPGLVDLIGRVRKI
                     AEGAALMTETSVQTKVISGVANILGNRPLEEALQAILEDLGPPHFDEEDRAFAAKIRK
                     TLTPQEIAAAWRAIGVEERDIALADFTVPLDSPRNPMIGSTDVGDVSWVVPTVQAHAP
                     TVAIGTAFHTWQIVAQGKQPAAHKAMTQVAKAMAALGARAVQDPDMVAAAKADLARRT
                     AKTPYRSPLPPDVQPPLDMSR"
     gene            complement(8368..>9661)
                     /locus_tag="PK812_07480"
     CDS             complement(8368..>9661)
                     /locus_tag="PK812_07480"
                     /inference="COORDINATES: protein motif:HMM:NF021589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRJ69435.1"
                     /translation="PSSSTTSWRPTARAPVAAAGTLPVVASTPQVVSPGLSEPVVAKR
                     KKPQQDDPYAPLGIRVGGLRVLPAIEGLAGYDTNALRGTSGTRYKGSRLYQVVPELSV
                     QSDWSRHALQMDLRGTYAWYQDVDDANRPTLDGKINFRADIRQDSTLTLELKEKIDSQ
                     RPGSTDLSGAVKGRPLYFVTSGSAAWAEKFGYATATTTATIDRYDYQDATTFSGATLS
                     QRDRNYTAYGLKLRGAYELTPGISPFVEVAGDVREHDEKHDAATYRRNSHGATAKIGS
                     TFELTRILTGEVSAGLGIRRYDDHRLPNLSGAIGDAALIWQMTPLTKVTGKVGTEFVE
                     TVQSGSPGAVSRKVSLEVAHDLLRNLTITGTAGFTHASYKGINRIEDTITAGLKADYK
                     IDRNWIVRGSYGYERALSNQTSSSYLSHIFMMGVRLQQ"
BASE COUNT         1724 a         3137 c         3012 g         1788 t
ORIGIN      
        1 tcgccgatct tcggcatgcc gatcctcgac gtggagcgcg cgaagaccgt gttcttcatc
       61 aagcgcggca tgtcctcggg ctacgccggc gtcgacaacg aactgttctt caaacccaac
      121 accatgatgc tgttcggcga tgccaagaag atgacggaag agatcgtcaa ggccatggcg
      181 cactgacggc tggtccggaa gtcctgaaag aagccgccgc agggcggctt tttcgcgccg
      241 ggcggcgccc gtcacttgcc ttcatcggga ttgccccgat aatcgcgcca tgaaaaagcg
      301 catcctgccc gctctgatcg ggcttctgct cgtggtctac atcgcaatcg ccgcctacat
      361 gggcgccttc cagcgctcgt tcatctatgc ggttgcgcca cagaaaccgg ccgtcgccgc
      421 tgcctccgtt cctggactga cagaaacgga cctcgtcatg gccgatggtg tccggctcaa
      481 cacctggatt gtgccgccga ccgctgggga tcggcccatc tatgtctatt tccatggcaa
      541 cgccaacacg ctgatgcgcc gcgccgaacg cttccgtctg ttgacgtcca acgggtctgg
      601 actgcttgcg atccactacc gaggctacgg aggatccggc gggaagccca cggaggcgtt
      661 gctccataag gatgcggagg cagccttcgc cgaagcggag aaacgatttc cgggccggaa
      721 actggttgcg ttcggggagt cgctcggctc aagcatcgcg atccgcctcg ccagcgagcg
      781 cccgcaggtc gcggccgtcg ttctcgactg ccccttcgtg tccgtactgt cgcgagcgca
      841 ggcctcgtac ccctggctac cggtgtcgct gctgctggtc gatacgtttc gctcggatcg
      901 ctggattggc acggtcaagg cgccgatctt catcatgcac ggcgcgctcg accgggtgac
      961 gccgatcgag gaaggccgca agctgtttgc actggcgccg gataggaagc gctttgtcgc
     1021 ctatccggac gccggacatg tcgacaactt ccgcttcggg gcggcggacg caatctggca
     1081 gttcctgaag gacatggctg tcattccggg ctgagccttg cgcccaggtt cctgctgtct
     1141 gagctccctt gggggaaggc atcaggcatc aggcgccatc agcccccctc aaccactcgc
     1201 aggctggtag cccaggccac gcatgctgtc gtcgagaggc ttcaccattt catagctgac
     1261 cggcgaatac cccgctgccc gatatgcgcc tagcgccaag gcgttgttga caacggcagc
     1321 gagggtaatc gctctggcat tgcccgcgag agccaccgcg tcagcatggg caagcagcat
     1381 gcggccatat ccacgtcgac ggttgctgcc agccaccacg atgttctcga tatgaaggat
     1441 cggacggtcg ctcttcggta gatagggcgc cgacttcggc caaaaatacg ccaggtatcc
     1501 gatcacggcc cgttcaactt cgagcacaac ctgaccgccc cccagcgcgc acttttccgt
     1561 gtcatcagcc aggcaagctt cggcgccggc tctgtccgtc ctgcgcggag cgccaacttg
     1621 ggcttcgtgc ctgttcaatt ccagcaacag agcgatgaca tcttgccgat cggtgggcgc
     1681 atagggccgg atcgacgatg gtgtcaccgg aaaaccccgc tccgctgccg gctcatgctc
     1741 tcgcgagcgc cgcgatggtt gggatcgatc gagagcgcgc gctgataggc ttcattcgcc
     1801 tcggcgcgct tgccctgctg atcgagcgcg tacccgcgcc ccgcccacgc gtcggcgttg
     1861 cggttatcca cgttgagcgc tgcggtgaaa tcctctgcgg cctgatcgaa gcggcccgtg
     1921 gcattgaggc tctgaccgcg cgcgacatag ggcggcgcga catagggatt gcgatcgacc
     1981 gctgcgtcga agtcagacat cgccagcgca tgctgtcccc ggcgctgata gaccagacca
     2041 cggccgtgat aggcctccgc tgcatcgggc ttgatacgga tgacatgcgt caggtcgttc
     2101 atcgccgcat cgaggttgtt ctgcgcgcgg tagaggttgc cgcgcgcgag ataggcctgc
     2161 gcgtagttcg gatcggcttg aatggcgcgg ttgaaatctg cgaaggcgag atcgttcttg
     2221 ttggcctgcc gataggcaag gccgcggttg acgaaggccg gcgcgaaatt cgggtcgagc
     2281 tggatcgcct tggagaaatc cgcgatggcg tcattgaagc ggctggcgcg cgcataggcc
     2341 gcgccgcggg tattgtaggc ttgcggatcg ttcggattgc gggcgatcac ttccgacagc
     2401 gaggagatgt tggcagaact tgcgccggcc gtgttggcat cgtcgatctc ggccacgccc
     2461 ggcggattga gcccgcccat ggtggtctcg caggcagaca gcgacagggc cagggccacc
     2521 gtgcccagaa catgcgccaa gcgaaaatgg tcgacaggaa tcatccggat cagcatggct
     2581 tggccgtaga ctgcaaatca ggcgaaaaat cgccggaaaa tatcaagagc gcccgctcga
     2641 tcgagggacg ctcccatgaa accgtatttt ggcggcgcag ggtttccaat tcattaaccc
     2701 agcgcaacaa gggcattcgt cagggcttct tgacctttgg cttcggcgcc gcgatcaggc
     2761 cctcgcgctg cagacgcttg cgggccagct tgcgcgccct gcgcacagct tccgcctttt
     2821 cgcgcgcgcg cttctcggag ggcttctcat aatggccgcg gagcttcatc tcgcggaaga
     2881 tgccctcgcg ctgcatcttt ttcttgagtg cgcgaagagc ctggtcgacg ttgttgtcgc
     2941 gaacgagtac ctgcacgttt tgtcccgtca atcctgcggt tggggcaaag aaaacggcgg
     3001 cctggagcaa ccgcccttca atcggaagcg cgcacatagc aaaggcgcac cggcttgtcc
     3061 atatccagac ggcgatttcc ggcatttgaa agcacgaagt cacccgctgc agccgccaga
     3121 tttccgcgcg gaggcggaat gccgcaggtt gaggcgtgtc ggagccttct agcgcgcaag
     3181 cttcaggcgt tccagcaaag ccggcgtcgc gtagccatcg ggcaccaggc cggcctgctt
     3241 ctggaaccgg atgatcgact cgcggctttg ctcgccgaag cgtccatcga tttttccctg
     3301 atacaggccg cgtttcgtca actggcgctg gacctccatg ccctggtcgc gcgtcaggcg
     3361 cttgtccttc ttcggccagt cagcctgcac cggttcgccg ccgtagatga cgtcgcccaa
     3421 caatcccacg gcgagcgcgt aggcatccga ggaattgtag gccttgatca ccttgaaatt
     3481 atcggtgagc agaaaggctg ggcccttgat tccggccggc aagaacagcg cggcctcacc
     3541 gcggcgcggc agggcctgcc cgctggcgcg cttgatgccg agcgcggaaa acgccgggaa
     3601 ggccttgcga ccaagcgcct gcctgaggtc gatgccatgc ggcagctcga cctccatgcc
     3661 ccagggcaag cccgtctgcc agccatgttc cttgaggtag ttggcggtcg atgccagtgc
     3721 gtcggggatc gagtcccaga tgtccttgcg tccgtcgcgg ttgtgatcga cggcatactt
     3781 catgaagctc gagggcatga actgggtgtg ccccatggca ccggcccatg acccacgcat
     3841 ctccgagcgc tcgacatgcc cctcttccag gattttcagc gcgataatga gttccttgcg
     3901 gaagaagtcg tcgcgatagc gaacgtaggc cagcgtggcg agcgcccgga tcaccgaatt
     3961 gttgccgaag aaggagccga aattcgtctc catgccccag atgcccatga ccgcccgccg
     4021 gtccacgcca tagcgactct caacggccga aaccgtgtcg gaccatcggc cgcccgcttc
     4081 cctgccccgt tcgacccggc cggaggtgat cgccccttcg acgtagtccc agatcgggcg
     4141 gacaaattcg gactgcttct tcgtctgggc gacaattttc tggtcaggcg tcacgccctt
     4201 gaaggcgcca tcgaacgtgg cgcgcgacac gccggccgcc tgcgcttccg gccacaggcc
     4261 gacgaggaag gccgcgaagt cggcacgcgc aggtgccagc ccgccaccca ggcacaaaag
     4321 cacaagaagc aggcgaaaca tcggcctcgc tacggttccg gagtagcgca tcaggtcacg
     4381 ccgcattgcg ggccttcagg tggcgctccc agcgcagcgc ctgctcgacg atccgccgca
     4441 gatcatcatg ttcgggcttc caacccagct ccgcgcgaat gcggtccgcc ttcgcgacca
     4501 cggcgggcga atcaccggcg cggcgcggca tggcccggac ttcgaaatcc accccgctca
     4561 cctgcttcac aacctcgatc acctggagaa cggaatagcc cgtgccatag ccgcagttgt
     4621 agaccgtgct ccgtccgccc cggcccaggt aatccagcgc cttgatatgg gcagcggcga
     4681 gatcgctgac ctggatatag tcacgcacac aagagccgtc gggcgtatcg taatccgtcc
     4741 cgtacaggtc gaggtgactg cgatgcccga gggcggtctg taccgccacc ttgataagat
     4801 gcgtggcatt gggggtcgac tggcccgtgc gtccgtaggg atcggcaccg gcgacattga
     4861 agtagcgtag cgccacatag cggaaatcat gggcattggc ggtgtcgcgc agcatgaatt
     4921 ccgtcatcat cttcgacgtg ccatagggcg acatcggcgc cagtggggtg ttctcggaca
     4981 ccggcatgac cttcggctcg ccgtatacgg ccgcagtcga cgaaaaaacg aaccgcttca
     5041 cgccggattg aacggcagcc tcgatcagcg aacgcgaggt caccgtattg gcgagatagt
     5101 agccgagcgg atccacgacc gaatcgggaa ccacgatctt ggcagcgaaa tggcagatcg
     5161 actcgatccc gtgctcggca acgatgcgct tgaccagggt ctggtcgccg gcatctcctc
     5221 cgaaaaacgg cacatccggg ggtaccgccc aggcaaaacc ggtggacagg ttgtcgagga
     5281 cgaccacctt ctgcccggtg tcccgcaagg catgcaccat atgactgccg atgtaaccgg
     5341 cgccgccagt aacgagaacg ctcatttcat cctcccggcc gccggccgta acattgtggt
     5401 agccctgtca gcgaagacta gcctcccaat tcggataccc ttgatccggt tcaagttgcg
     5461 ttaagccctt gtcagccatt agccgcgtgc gtcgctgcaa acagggttcc ctgcaagcat
     5521 ggcgcgttca aggcaaacac gacggattcc ccgatgcagc ccaagaagat tcgcaaagcc
     5581 gtgttcccgg tcgccggtct cggcacccgt ttcctgcccg ccaccaagtc ggtgccaaag
     5641 gagatgatga acgtcgtcga ccgaccggtg ttgcaatggg tggtggacga ggcacgggaa
     5701 gccggcatcg agcacctcat cttcgtcacc ggccgcaaca aggccgtgat cgaggaccat
     5761 ttcgacatcg cctacgaact ggaagcgacc ctaaaggagc gcggcaagct caaggaactt
     5821 gaagcgctgc agaaagacct gccgccggcc ggcatggcct ccttcacccg ccagcaggcc
     5881 ccgctgggtc tcggccatgc cgtctggtgc gcgcgcgatc tcgtcggcga cgaacccttc
     5941 gccgtcatgc tgccggacat gctgtccagg ggctgcatga agcagatggt ggacgcctat
     6001 gagaagcatg gcggcaacgt catcgcgctc gaacaggtgc cgatggacca gacgcaccag
     6061 tatggcatcg tcgcggtcgg gcaggcgcac ggtcgtgtgc atgaactgac gggcatggtg
     6121 gaaaagccca agccgggcac cgccccatcg aactacatca tttccggccg ctacattctc
     6181 cagccagaag tgttctcgat cctctcagcc aaggagaagg gcgctggcgg cgaaatccag
     6241 cttaccgacg cgatgatcaa gctgatgggc aagcagaggt tcttcggcat gcactacgat
     6301 ggccgtacct acgacaccgg ctcgaagatc ggcttcctcg ctgcgaacgt cgcctacggt
     6361 ctggcccgcg ccgaattgaa ggacgagttc cgggcggaaa tcgagcgcat tctcgcaggg
     6421 aagtagtttc tcgtccgggc gctgagcacg acgggcgcgg gatcagacct cggcccgttt
     6481 gcgtttggcc ctggctcttg cctcgccccg gttgcgaatc atcaggaact ttccggccaa
     6541 gacgccggga atggtccagg tcccctcgat ctcgtcgccg gctccattga gccggccatc
     6601 atagatcaca tcctgccagt cctcgatggc cggatcgtag gtcttcacga agttgacggc
     6661 ctcgccctgg cgggtccctt ccagcgttgc gaacaaggcg gcgtcgccca tatcggtctc
     6721 gatgttttcg tgcgtcgtgc cgctcagcat ctcgccgaat tggataagcg ttgcgacgaa
     6781 ggcaacgggc ggaacctgag gcgcgggata ggaatactgc ccatgccaga tgcccgtcag
     6841 gctgtcgcgg tccacctttt cattcacggc gccgtcaccg cgacatgtcg agcggcggct
     6901 gcacgtccgg cggcagcggc gaacgatagg gcgtcttcgc tgtgcggcgg gcaaggtctg
     6961 ccttggcggc ggcgaccata tcagggtcct gaacagcccg ggcgccaagt gccgccatag
     7021 ccttcgcgac ctgagtcatg gccttgtgcg ccgccggctg cttgccctgc gccacgatct
     7081 gccaggtgtg gaaggccgtg ccgatggcaa cggtcggcgc gtgggcctgg acggtcggca
     7141 ccacccagct gacatcgccg acatcggtcg agccgatcat tggattgcgt ggcgagtcga
     7201 gcggcacggt gaagtcggcg agcgcgatgt cgcgttcctc gacgccgatg gcccgccagg
     7261 ccgcagcgat ctcctgcggc gtcagggtct tgcggatctt cgcggcgaac gccctatcct
     7321 cttcgtcgaa gtgcggcggc cccagatctt ccaggatcgc ctgcaacgcc tcctccagcg
     7381 gccgatttcc gaggatgttg gcgacaccgc tgatcacctt ggtctggacg ctggtctcgg
     7441 tcatcagcgc cgcgccctcg gcaattttgc gcacgcggcc gatcaggtcg acgagaccgg
     7501 gcaggtcgcg cgcccggata agatagcgca ccttcgcact tgcctgcacc acgttcggcg
     7561 cgatgccgcc cgtatcgagg atggcggaat gcacgcgtgc gtcgctcggc atgtgctcgc
     7621 gcatgtaatt gacgccgacg ctcatcagtt cgaccgcatc gagcgcgctg cgccccagtt
     7681 ccggcgaagc ggcggcatgc gcggcgcggc ctgtgaaggt gaagtcgatg cgggtattgg
     7741 ccagcgacag cggcggcgtc acctcccaga aggaataggg atgccaggtg atggcaatgt
     7801 ccgcatcctt gaacacgccg gcacgcacca tgaaggcctt ggcggcgccc ccttcctcgg
     7861 ccgggcaccc atagtaacgc acgcgtccag gcagcttgtt ctcggccaac cagttcttca
     7921 gtgccgtcgc cgccagcatg gcggccgagc ccagcagatt atggccgcag ccgtggccat
     7981 ggccgcccgc ctcgatcggc cggtgctcgg cgacgccggc ttcctgcgac agccccggca
     8041 gcgcatcata ctcgcccagg aatgcgatga cgggaccgcc ctctccggcc tcgccgatca
     8101 cggccgtcgg aatgcccgcg acattccggg taatgcggaa cccctccacg ctgagctgct
     8161 cgatatgttc cgcaaccgag cgcacctcgg tatagcagac ttccggcatg ccccagaccc
     8221 ggtcggcgag cgcgacgaac cgttcgcgcg cctgatccac ctgacgccag accgagcttc
     8281 gattgtccat gagtgcagtt cctgtccgcg ccgggggcgt ctgatcgatg caaggctacc
     8341 cgcctcgtcg cgcggcttca agccatgcta ctgttgcagg cgaacaccca tcatgaagat
     8401 gtgactgagg tagctcgaac tggtctggtt cgacagggca cgctcgtagc cataacttcc
     8461 gcgcacgatc cagttgcgat cgatcttgta atccgccttc agcccggccg tgatcgtgtc
     8521 ctcgatacgg ttgatgccct tgtaactggc atgcgtgaag ccggccgtgc ccgtgatcgt
     8581 caggttgcgc aacaggtcat gcgcgacttc gaggctgact ttccggctca ccgctcccgg
     8641 tgatccgctc tggaccgttt ccacgaattc cgtgccgacc ttccccgtca ccttcgtcag
     8701 gggcgtcatt tgccagatca gggcggcgtc gccgatcgcg ccgctcaggt tgggcaatcg
     8761 atggtcatcg tagcgccgta tgcccaaacc ggcggacact tcacccgtca ggatgcgcgt
     8821 aagttcgaaa gtcgagccga tcttggccgt ggccccgtgc gaattccgcc gataggtcgc
     8881 ggcatcgtgc ttttcgtcat gctcgcggac atcgccggcc acctcgacga agggggatat
     8941 tccgggcgtc aattcatagg cgccgcgcaa cttgagcccg taggccgtat agttgcgatc
     9001 cctctgacta agcgtggcgc ccgaaaacgt cgtcgcgtcc tgatagtcgt agcggtcgat
     9061 cgtcgccgtc gtggtcgcgg tggcgtagcc gaacttctcg gcccaggctg ccgaacccga
     9121 ggtcacgaaa tacagcggac ggcctttgac cgcgccggac aggtccgtgc tgcccgggcg
     9181 ctgggaatcg atcttctctt tcaattcgag ggtcagggtc gaatcctgcc ggatgtccgc
     9241 tcggaagttg atcttcccat cgagcgttgg acggttggcg tcgtccacat cctggtacca
     9301 ggcgtaggtg ccacgtaggt ccatctgcag ggcatggcga gaccagtcgc tctggacact
     9361 cagttcagga acgacctgat aaagcctgga ccccttgtag cgggtgccgg aggtgccacg
     9421 cagggcattg gtgtcgtagc ccgccagccc ttcgatcgcc ggcagaaccc tgagtccccc
     9481 gacgcgaatg ccgagtggcg cgtaaggatc gtcctgctgc ggtttcttcc tcttcgccac
     9541 gaccggttcg ctcaggcccg ggctgaccac ctggggcgtg ctggcaacga caggaagcgt
     9601 tccggcggcc gccaccggag cgcgcgccgt tggccgccac gacgttgttg acgatgaggg
     9661 g
//
LOCUS       DAOMXN010000083         9518 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_3798_length_9518_cov_12.927507, whole genome shotgun sequence.
ACCESSION   DAOMXN010000083 DAOMXN010000000
VERSION     DAOMXN010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 9518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9518
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3798_length_9518_cov_12.927507"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="PK812_07485"
     CDS             <1..878
                     /locus_tag="PK812_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HRJ69436.1"
                     /translation="VRAAVRRPDLAGHLQPLGAVGQIQAVQANLRYPASVMRAIEGAD
                     AVVNLVGILAESGRQTFGAVQAQGARSVAEAAAKAGVRQLVQMSALGADRDSISDYAA
                     SKAFGEAATLSAVPSAVILRPSIIFGPEDQFFNRFAALATLSPALPLVGGGETKFQPV
                     YVGDVAAAIVAALEGRARAGMTYELGGPEVATFRALLEYILAETGRSRLLVPLPFWAA
                     RLKASVLQLLPGKLLTVDQVNLLQSDNVVGSAAIAEGRTLEGLGLTATAYESIVPSYL
                     YRFRKHGQFERIRSA"
     gene            complement(909..1901)
                     /locus_tag="PK812_07490"
     CDS             complement(909..1901)
                     /locus_tag="PK812_07490"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HRJ69437.1"
                     /translation="MSKIRPVVVVTRKLPDVVEARMAELFDVRLRTDDAPMSPDGLVA
                     AMQDADVLVPTVTDSITDSIIAGAPARLKLIANFGNGVDNIDVAAARRKGITVTNTPG
                     VLTEDMADFAMALIVAVARRFSEGARVIPQGQWHGWSPNWMLGRRVAGKRLGIVGMGR
                     IGQALAKRARAFGLQVHYHNRRPVSPRIEEELQATYWESLDRMLARMDIVSINCPHTP
                     ATFHLLSARRLKLLKSDAILINTARGEIVDTDALADLLETDHLAGAGLDVYEHEPAVP
                     ERLVRLADAGRVLLLPHMGSSTVESRVEMGEKVIINIKTYIDGHTPPDRVLPGL"
     gene            2076..2594
                     /locus_tag="PK812_07495"
     CDS             2076..2594
                     /locus_tag="PK812_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRJ69438.1"
                     /translation="MRNVRLSRLLCLLGLIASPVMASGTDNTGTVTGLPIPRFVSLKP
                     SDTPMREGPSKDHRIKWVFKREGLPVEIMAEFDTWRRVRDSEGTEGWVFHSRLSSRRT
                     ALVAPWSKEKTLPLYEREGEGNAVVARLQPGVLANIESCNGRWCRVNGEGFRGWMKQD
                     FMWGVYPGEAVR"
     gene            complement(2613..3059)
                     /locus_tag="PK812_07500"
     CDS             complement(2613..3059)
                     /locus_tag="PK812_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HRJ69439.1"
                     /translation="MTSMNAGAARSGCLVHELKGMLRQAGLRPTRQRIALGWLLFGKG
                     DRHVTAESLFEEATKARVPVSLATIYNTLHQFTEAGFLREVAVEGSKAYFDTNVGNHY
                     HFYIEGEGRLVDIEDADIALGQLPEPPEGMEIARIDVVVRLRRKAS"
     gene            3261..3788
                     /gene="fabA"
                     /locus_tag="PK812_07505"
     CDS             3261..3788
                     /gene="fabA"
                     /locus_tag="PK812_07505"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016769580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     3-hydroxyacyl-[acyl-carrier-protein] dehydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabA"
                     /protein_id="HRJ69440.1"
                     /translation="MTGSRPERKSSYTYDELLACGRTELFGPGNAQLPLPPMLMFDRI
                     TEISETGGAFDKGYIRAEFDIKPDHWFFPCHFQGNPIMPGCLGLDAMWQLTGFFLGWL
                     GEPGYGMALSTGEVKFKGMVTPSTKLVEYGIDFKRVMRGRLVLGIADGWLKADGEKIY
                     QATDLKVGLSKQKAA"
     gene            3818..5044
                     /gene="fabB"
                     /locus_tag="PK812_07510"
     CDS             3818..5044
                     /gene="fabB"
                     /locus_tag="PK812_07510"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HRJ69441.1"
                     /translation="MRRVVVTGMGIVSSIGNNTQEVLASLREAKSGIALSPSFVEHGF
                     RSQVAGAPTCVPEETVDRRAMRFHGGGTGWNHVAMDQAIRDAGLEQSDIVSERTGIIM
                     GSGGPSTRTLIEAVDKARESGNSKRVGPFAVPKAMSSTASATLATWFKIKGVNYSISS
                     ACATSNHCIGNAYELIQWGKQDVIFAGGCEELDWTLSVLFDAMGAMSSKYNDRPSTAS
                     RAYDANRDGFVIAGGAGVVVLEEYERAKARGAKIYGEIVGYGATSDGYDMVAPSGEGA
                     VRCMRQAMQGFKGKIDYINPHGTSTPVGDIKEIEAIREVFGSGEKAPLISATKSLTGH
                     SLGATGVQEAIYSLLMLNNDFVCESAHIETLDPAVADMRIVQKRIDNAGLGAILSNSF
                     GFGGTNATILMKRLEA"
     gene            5049..5321
                     /locus_tag="PK812_07515"
     CDS             5049..5321
                     /locus_tag="PK812_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HRJ69442.1"
                     /translation="MLFMVIEHFDQAKVKDIYNRFHEKGRMIPDGLSYVNSWISADFS
                     RCFQVMECDDASKIQEWVLVWGDLARFEVVPLVGSKETYEAVKKHL"
     gene            5338..6141
                     /gene="fabI"
                     /locus_tag="PK812_07520"
     CDS             5338..6141
                     /gene="fabI"
                     /locus_tag="PK812_07520"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HRJ69443.1"
                     /translation="MTSLMQGKRGLIMGVANSHSIAWGIAETLAKHGAELAFTFQGEA
                     LGKRVKPLAESLGSSLVIPCDVEDVASVDAVFETLKAAWGRLDFIVHAIGFSDKNELK
                     GRYADTSRENFSRTMVISCFSFTEVAKRAADLMTEGGSMITLTYGGSTRVMPNYNVMG
                     VAKAALEASVRYLAADFGPKGIRVNALSPGPVRTLAGAGIADARAMFSFQQKHSPLRK
                     SVSLEEIGGSALYFLSDLSGGVTGEVHHIDAGYNIISMPRPEDFGPEGR"
     gene            6236..6604
                     /locus_tag="PK812_07525"
     CDS             6236..6604
                     /locus_tag="PK812_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HRJ69444.1"
                     /translation="MLRLALGFAALIALPSAASAQSLIGTWTRDSGASKVTFAKCGEA
                     VCGTLSWLKDTSGKAKVGQRVFSNLKETAPGKYSGTAIDPEDGKQYNGTITVTGNTMK
                     GGGCVLGGMICRTVVYNRGG"
     gene            complement(6784..8001)
                     /gene="trpB"
                     /locus_tag="PK812_07530"
     CDS             complement(6784..8001)
                     /gene="trpB"
                     /locus_tag="PK812_07530"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HRJ69445.1"
                     /translation="MNAQDRPNSFRSGPDERGFFGLFGGRFVAETLMPLVLELEKAYA
                     DAKADPAFKAEFDSMAKHYIGRPSPLYFAERMTAHLGGAKIYFKREELNHTGAHKVNN
                     VLGQILMARRMGKPRIIAETGAGQHGVATATLCARYGLPCVVYMGAVDVERQKPNVFR
                     MKMLGAEVVPVQSGSKTLKDAMNEALRDWVTNVENTFYCIGTVAGPHPYPAMVRDFQS
                     VIGQETRSQILEAEGRLPDSLVAAVGGGSNAIGLFHPFLDDPGIEIYGVEAAGHGIAT
                     GQHAASIAGGRPGVLHGNRTYLLMDGDGQIAEAHSISAGLDYPGIGPEHSYLNDVRRV
                     VYRPVTDAEALEAFQLCSKLEGIIPALESAHALAEVIKLAPTKPKDHLMVINVSGRGD
                     KDIFTVAEHLGGM"
     gene            complement(8005..8691)
                     /locus_tag="PK812_07535"
     CDS             complement(8005..8691)
                     /locus_tag="PK812_07535"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HRJ69446.1"
                     /translation="MSLLVKICGLSTPETVAAARDAGADMLGFVFFPKSPRHVALRDA
                     QPLFGLARTISAFRKSARPCRIVALVVNAGQPLLDDIACELTPDFFQCHGSETPLEVA
                     HIANDPGTPVIKALGVSAADDLAAAPAYAAAGATILIDAKPPKDAAYPGGHGKPFDWT
                     ILRGLDPGLPFMLSGGLTPETVGEAIRTVRGFGVNLVGVDVSSGVETAPGVKDVDRIR
                     AFVAAVRQAE"
     gene            complement(8688..9020)
                     /locus_tag="PK812_07540"
     CDS             complement(8688..9020)
                     /locus_tag="PK812_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HRJ69447.1"
                     /translation="MRAFLKLVILVPLAVLVVLLAMANRTPVTLSVDPFVAGLPLLQI
                     TVPLYVAILGAVMAGIVIGGIASWFGQGKHRKAARFNRRECEKLKAEADRLKLMLPAT
                     AALPPAGR"
     gene            complement(9042..9356)
                     /locus_tag="PK812_07545"
                     /pseudo
     CDS             complement(9042..9356)
                     /locus_tag="PK812_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186701.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
BASE COUNT         1642 a         3090 c         3090 g         1696 t
ORIGIN      
        1 gcgtgcgcgc cgcggtgcgc cgccccgacc tggcgggaca cctccagccg ctcggcgccg
       61 tggggcagat tcaggcggtt caggccaacc tgcgctaccc tgcgtccgtg atgcgggcca
      121 tcgagggcgc ggatgcggtc gtcaatctgg tcggtatcct cgcggaaagc ggccggcaga
      181 ccttcggcgc ggtccaggcg cagggcgcgc gcagcgtggc cgaagcagcg gcgaaggccg
      241 gcgtccgcca gctggtgcag atgtcggcgc tcggagcgga ccgcgattcg atctccgact
      301 acgccgcatc gaaggctttc ggcgaggctg caaccttgtc cgccgtgcct tcggctgtga
      361 tcctgcgtcc ctcgatcatc ttcggcccgg aagaccagtt cttcaaccgc ttcgcggccc
      421 tggccacgct ctcgccggca ttgccgcttg tcggcggtgg cgagacgaag ttccagccgg
      481 tctatgtcgg cgatgttgcc gccgcgatcg tcgccgccct cgaaggacgg gcccgcgccg
      541 gcatgaccta cgaactgggc ggcccggagg ttgcgacctt tcgcgccctg ctggagtata
      601 tcctggcaga gaccgggcgc agccggctgc tggttcccct gcccttctgg gcggcgcggc
      661 tcaaggcgag cgtgctgcaa ctgctgccag gcaaactgct gacggtcgat caggtcaatc
      721 tgctgcaaag cgacaacgta gtcggttctg cggcgatcgc cgagggccgc acgctggaag
      781 gcctcggcct taccgccacg gcctacgaat cgatcgtgcc gagctacctc taccgcttcc
      841 gcaagcacgg ccagttcgag cgcatccggt ccgcctgaag tctgatcggt tccggacgcg
      901 actgggggtc aaaggcccgg cagcacccgg tcgggcggcg tatggccgtc gatgtacgtc
      961 ttgatgttga tgatgacctt ctcacccatc tccacacggc tttccaccgt ggacgagccc
     1021 atgtgcggca gcagcagcac gcgtccggcg tccgccagcc gcaccaggcg ctcgggcacg
     1081 gccggctcgt gctcatagac atcgaggccg gcgcccgcga ggtggtcggt ttccagaagg
     1141 tcggccagcg cgtcggtgtc gacgatctcg ccacgcgccg tgttgatgag aatcgcgtcg
     1201 gacttcagca gcttgagtcg gcgcgccgaa agcagatgga acgtcgccgg cgtatgcggg
     1261 cagttgatgg acacgatgtc catgcgtgcg agcatcctgt ccaggctctc ccagtaggtc
     1321 gcctgcaatt cttcctcgat gcgtggcgag accggccggc ggttgtggta atgcacctgc
     1381 agcccgaacg cgcgcgcgcg cttcgccagt gcctggccga tacgccccat gccgacgatg
     1441 ccgagacgct tgccggcaac gcgccggcca agcatccagt tcggcgacca gccgtgccac
     1501 tggccctgcg gaatgacgcg cgcgccctcg gaaaagcgac gtgcgaccgc gacgatgagc
     1561 gccatggcga aatcggccat gtcctccgtc agcacgccgg gcgtgttggt gactgtgata
     1621 cccttgcgcc gcgccgcggc cacatcgatg ttgtcgacgc cattgccgaa attggcgatc
     1681 aacttgaggc gggccggcgc gccggcgatg atgctgtcgg taattgagtc cgtcacggtc
     1741 ggcactagga cgtcggcgtc ctgcatggcg gcgacaagcc cgtcggggct catgggcgca
     1801 tcatccgtcc gcaggcggac gtcgaacaac tcggccatgc gcgcctcgac cacatcggga
     1861 agtttgcgcg tcacgacgac gacaggccgt attttcgaca tcgtctcctc gcctgtatcg
     1921 gcactggcgg tgcgccgtgc tcaacccaac gttaaccgcg ttggcgcagt gtgccggaac
     1981 ctattatgtt gcaatttgcg gctctaacag atcgctggcc ctgcgcaagg cgcatggcct
     2041 gtatgagcaa gccgcagtct cggttggtag tccgcatgcg aaacgtccgg ctctctcgcc
     2101 tgttgtgcct tctcggcctc atcgcgtcgc ccgtcatggc ctccggcacg gacaataccg
     2161 gaactgtcac cggactgccc attccccgct tcgtcagcct gaagccgagc gacacgccga
     2221 tgcgcgaggg tccctcgaaa gatcatcgca tcaaatgggt gttcaagcgt gaggggctgc
     2281 cggtcgagat catggccgag ttcgatactt ggcgccgcgt ccgcgattcc gaggggaccg
     2341 agggttgggt tttccattcc cgtctgtcga gccggcgcac tgcgctggtc gcgccctggt
     2401 ccaaggaaaa gaccctgccg ctttatgagc gcgagggcga aggcaatgcg gtcgtggcgc
     2461 gccttcagcc cggcgtcctg gccaacatcg agagctgcaa cggccgctgg tgccgggtca
     2521 acggcgaggg cttccgcggc tggatgaagc aggacttcat gtggggcgtc tatccgggcg
     2581 aagcggtgcg ctgagcccac ggacttcgga cgtcacgagg ccttgcggcg caggcgcacg
     2641 accacatcga tccgggcgat ctccatccct tccggcggct ccggcaactg ccccagcgcg
     2701 atatcggcat cctcgatgtc gaccaggcgt ccttcgccct cgatatagaa gtgatagtga
     2761 ttgccgacgt tcgtgtcgaa ataggccttc gatccttcga cagcgacttc gcgcaggaag
     2821 ccggcttccg tgaactgatg cagggtgttg tagatcgtcg cgagggacac cggcacgcgg
     2881 gccttggtgg cttcctcgaa cagcgattcg gccgttacgt gacggtcgcc cttgccaaac
     2941 aggagccaac ccaaggcgat gcgctggcga gtcgggcgca ggccggcctg ccgcagcatg
     3001 cccttcagtt cgtgaacaag gcagccgctg cgggccgctc ctgcattcat cgatgtcata
     3061 ggcttacgtg tctcgcaaaa cgtaggtcgt caccataaac atagcgttcc cgtgggtttt
     3121 ctgcaacgcc gcagccgatc ctgtggggac cggcgcattt tgtggcgggg tcgtgctttc
     3181 gggggctgtc acagcgtgtt acacgaggtt acaattcgtg aaatcgacaa gagccgccca
     3241 acttcaggat cgcgttgccc atgactggtt cccgtcccga gcgcaaatcc agctatacct
     3301 acgacgaact gctggcctgt ggccgaaccg agttgttcgg acccggcaac gcgcaattgc
     3361 cgctgccgcc gatgctgatg ttcgaccgga tcaccgagat ttccgagacc ggcggcgcgt
     3421 tcgacaaggg ctacatccgc gccgaattcg acatcaagcc ggaccattgg ttctttccct
     3481 gccacttcca gggcaacccg atcatgccgg gctgccttgg tctcgacgcg atgtggcagt
     3541 tgactggctt ctttctcggc tggctcggcg agcctggcta tggcatggcg ctgtcgaccg
     3601 gcgaggtgaa gttcaagggc atggtcacgc cctcgacgaa acttgtcgag tacggcatcg
     3661 acttcaagcg cgtcatgcgc ggccgtctgg tgctgggaat tgccgacggc tggctcaagg
     3721 ccgacggcga gaagatttat caggcgaccg atctcaaggt cggcctgtcc aaacagaaag
     3781 cggcctgacc gggccggtcc agagacgagg gaacgccatg agacgtgttg tcgtaaccgg
     3841 aatggggatc gtctcctcca tcggcaacaa cacccaggaa gtgctggcct cgctgcgcga
     3901 ggcgaagtcc ggcattgccc tgagccccag cttcgtcgag cacggcttcc gcagccaggt
     3961 ggccggcgcg ccgacctgcg tgccggagga gacggtcgac cgccgcgcca tgcgcttcca
     4021 cggcggcggc accggctgga accacgtcgc catggatcag gcgatccgcg atgccgggct
     4081 ggagcaatcc gacatcgtca gcgagcgcac cggcatcatc atggggtcgg gcggtccgtc
     4141 cacgcgcacc ctgatcgagg ctgtggacaa ggcgcgcgag agcggcaatt ccaagcgcgt
     4201 cggacctttc gccgttccga aggccatgtc ctcgaccgcc tcggcgacac tggccacctg
     4261 gttcaagatc aagggcgtca actattcgat ctcatcggcc tgcgcgacat cgaaccactg
     4321 catcggcaac gcctacgaac tgatccagtg gggcaagcag gacgtgattt tcgccggcgg
     4381 ttgcgaggag ctggactgga cgctctccgt cctgttcgac gccatgggcg cgatgtcgtc
     4441 caagtacaac gaccgcccct cgaccgcctc ccgcgcctac gacgccaacc gcgatggctt
     4501 cgtcatcgcc ggcggcgcgg gcgtcgtcgt cctggaggag tacgaacggg caaaggcgcg
     4561 cggcgcgaag atctacggcg aaattgtcgg ctacggcgcg acgtcggacg gctacgacat
     4621 ggttgcgccg tcgggcgagg gcgcggtgcg ctgcatgcgc caggccatgc agggcttcaa
     4681 gggcaagatc gactacatca acccgcacgg cacctcgact ccggtcggcg acatcaagga
     4741 gatcgaggcg atccgcgagg tgttcggctc gggcgagaag gcgccgctga tctcggcgac
     4801 gaagtcgctc accggccatt cgctgggcgc gaccggcgtg caggaggcga tctattcctt
     4861 gctgatgctg aacaacgatt tcgtgtgcga gagcgcccat atcgagaccc tcgatccggc
     4921 cgtcgccgac atgcgcatcg ttcagaagcg catcgacaat gccggcctcg gggcgatcct
     4981 gtccaattcc ttcggcttcg gcggcaccaa cgccaccatc ctgatgaagc ggctggaggc
     5041 ttgatctgat gctgttcatg gtgatcgaac atttcgacca ggcgaaggtg aaggacatct
     5101 acaaccgctt ccacgagaag gggcggatga tcccggacgg gttgtcctac gtgaacagct
     5161 ggatctcggc cgatttctcc cgctgtttcc aggtcatgga atgcgatgac gcgtccaaga
     5221 tccaggaatg ggttctggtc tggggcgacc tcgcccgatt tgaagtcgtg ccgctcgtgg
     5281 gctccaagga gacctacgag gcggtgaaga agcatctttg aaagacaccc cgatcagatg
     5341 acttccctca tgcaaggaaa gcgcggcctc atcatggggg tcgcaaacag tcattccatc
     5401 gcctggggca tcgccgagac gctggcgaag cacggggcgg aacttgcctt caccttccag
     5461 ggtgaggcgc tgggcaagcg cgtgaagcct ctcgccgaat cgctcgggtc gtcgctggtg
     5521 atcccctgcg atgtcgagga tgtcgcctca gtcgacgccg tgttcgagac gctgaaggcg
     5581 gcctggggca ggcttgactt catcgtccac gccatcggct tttccgacaa gaacgagctg
     5641 aagggccgtt acgccgacac cagccgcgag aatttctcgc gcaccatggt gatctcctgc
     5701 ttctccttca cggaagtcgc gaagcgcgcc gctgacctca tgaccgaggg cggttcgatg
     5761 atcaccctca cctacggcgg ctcgacgcgc gtgatgccca actacaacgt catgggcgtc
     5821 gccaaggcgg cgctggaagc gagcgtgcgc tatctcgccg ccgatttcgg gccgaagggc
     5881 atccgcgtca acgcgctctc gcccggtccg gtgcgcacgc ttgccggcgc cggcatcgcg
     5941 gatgcgcggg cgatgttctc cttccagcag aagcactcgc cgctgcgcaa gtccgtgagc
     6001 cttgaggaga tcggcggctc cgcgctctat ttcctgtccg acctgtcggg cggtgtgacc
     6061 ggcgaggtgc atcacatcga cgccggctac aacatcatct ccatgccgcg cccggaggat
     6121 ttcggccccg agggccgatg atcggtctgt gccctcttgc cagatcacgc gattggccta
     6181 gcatcgcctc gcacaggccg gtggcgacat accggcgcgt ctctgggagg aactcatgct
     6241 tcgtcttgcc ttgggcttcg ccgccctcat cgccctgcct tccgccgcct cggcgcagtc
     6301 tctgatcggc acgtggacac gcgatagcgg cgcctccaaa gtgacgttcg cgaaatgcgg
     6361 ggaggccgtc tgcggcacgc tctcctggct gaaggatacg agcggcaagg ccaaggtcgg
     6421 ccaacgcgtg ttctccaacc tgaaagagac cgcgcccggc aaatactccg gcaccgcgat
     6481 cgatccagag gacggcaaac agtacaatgg cacgatcacc gtcaccggca acaccatgaa
     6541 gggtggcggc tgcgtgctgg gcggcatgat ctgccgaacc gtggtctaca accgcggcgg
     6601 ctaaagcaat atccgaccag attggtattg ctctaagcgg cgcgcgatgc ggttccgggg
     6661 ccgcgaagcg gaccctggag ccacatcgtt tcgtcgaaga tcatgcactc ggacggattg
     6721 cggggctcga accttcggag cgccccggcg cgatgcgcct attcgaacca cgggggttcg
     6781 gattcacatc ccgccgaggt gttccgccac ggtgaagatg tccttgtcgc cacgcccgga
     6841 gacattgatc accatgaggt ggtccttggg cttggtcggg gcgagcttga tcacctccgc
     6901 cagcgcatga gcgctctcga gcgcgggaat gatgccttcg agcttcgaac agagctggaa
     6961 cgcctccagc gcctcagcat cggtgaccgg gcggtagacc acgcgcctga cgtcgttgag
     7021 ataggaatgc tccggcccga tgccgggata gtcgagcccg gccgagatcg aatgcgcctc
     7081 ggcgatctgg ccgtccccgt ccatcagcag ataggtccgg ttgccgtgca gcacaccggg
     7141 cctgccgccg gcgatcgacg ccgcatgctg gccggtggcg atgccgtggc cggctgcctc
     7201 gactccgtag atctcgatgc caggatcgtc caggaaggga tggaacaggc cgatggcgtt
     7261 ggaaccgccg ccgacggccg cgacgagact gtccggcagc cggccttccg cctccaggat
     7321 ctgggaccgc gtctcttggc cgatcactga ctggaagtcg cgcaccatcg ccggataggg
     7381 gtgcggcccc gctaccgtgc cgatgcaata gaacgtgttc tcgacattgg tgacccagtc
     7441 gcgcagggcc tcgttcatcg cgtccttgag cgtcttcgag ccggactgga ccggtaccac
     7501 ctcggcgccg agcatcttca tgcggaagac gttgggcttc tgccgctcca cgtccaccgc
     7561 acccatgtag acgacgcagg gcaggccata gcgcgcgcag agcgtggcgg tggcgacacc
     7621 gtgctggccc gcgccggttt cagcaatgat gcgcggcttg cccatgcgcc gggccatcag
     7681 aatctggcca agcacattgt tcaccttatg ggcgccggta tgattcagct cctcgcgctt
     7741 gaaataaatc ttcgccccgc cgagatgggc ggtcatccgc tcggcgaagt agagtgggga
     7801 aggccggccg atgtagtgct tcgccatgga gtcgaattcc gccttgaagg cgggatcagc
     7861 cttggcgtcg gcataggcct tttccagctc cagaacgagc ggcatcagcg tctcggcgac
     7921 gaagcgcccg ccgaagaggc cgaagaagcc gcgctcgtcc ggcccggagc gaaaggagtt
     7981 gggtctgtcc tgcgcgttca tgtctcactc cgcctgtcgc acggccgcga cgaaagccct
     8041 gatcctgtcc acgtccttca cgccgggcgc ggtctcgacg ccggacgaca catcgacgcc
     8101 gacgaggttg acaccgaagc cgcgcacggt ccggatggcc tcgccgacgg tctcgggcgt
     8161 cagcccgcct gacaacatga agggaagccc ggggtcaagg ccgcgcagga tcgtccagtc
     8221 gaagggcttg ccgtgcccgc ccggataggc cgcatccttc ggcggcttcg cgtcgatgag
     8281 gatcgtcgcg ccggctgcgg cataggctgg cgcggccgcg agatcgtccg cggcggaaac
     8341 gcccagagcc ttgatcaccg gcgttcccgg atcgttggcg atatgggcga cctccagcgg
     8401 cgtctcgctg ccatgacact ggaaaaagtc cggcgtcagc tcgcaggcga tgtcgtcgag
     8461 cagcggctgt ccggcattca cgaccagggc cacgatcctg cagggccggg cggacttgcg
     8521 gaacgcggag atggtccttg caagtccgaa gagtggttgc gcatcccgca gggccacatg
     8581 gcgcgggctc ttcgggaaga acacgaagcc cagcatgtcg gcaccggcat cgcgggccgc
     8641 tgcgaccgtt tcgggtgtcg acaggccgca gatcttgacg agaagactca ccgcccggcc
     8701 gggggcaggg ccgccgtcgc cggcaacatg agcttcagcc gatcggcctc cgccttcagc
     8761 ttctcgcatt cgcgtcgatt gaaacgggcc gccttgcggt gcttgccctg accgaaccag
     8821 gacgcgatgc cgccgatgac gataccggcc atgacggcgc cgagaattgc gacataaagc
     8881 ggcaccgtga tctgcagcag cggcagaccg gctacgaagg ggtcgaccga cagggtcacc
     8941 ggcgtgcgat tggccatggc caacagcacg acgagcacag ccagcggcac gaggatgacg
     9001 agtttgagaa acgcgcgcat ggaacggatt tcctgtcggg atcagttctt cttagcggcg
     9061 gctttcgctg gcgccttgtt caggcgcacg cgcatctcct tgccgctctt gaagtagggc
     9121 acgacctttt cggacacgtt gacctgcgtc ccggtgcgcg gattgcggcc gacgcgcgcc
     9181 tcgcgcttct tgacggagaa ggcaccgaag ccgcgcaatt ccacccggtc accacgcttg
     9241 agcgcctcga cgatctcgtc gaggatcgca ttgaccatgt tctccacgtc ccgctggtag
     9301 agatgggggt tctgctcagc gatcttgagg acgagttcgg acttgatcat cgcgccgctc
     9361 ctttgtgcct tttggtcttc gcgccgggct tgccggcccg acggccggaa aaaccgatga
     9421 ctgtcttttc agcgccttga aaaaccgtca gttttccttg ggaggctgcc agaccgccaa
     9481 tagcccgtca agcgcatgca aatcaaggtc gcgccgcg
//
LOCUS       DAOMXN010000084         9479 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_3818_length_9479_cov_11.130730, whole genome shotgun sequence.
ACCESSION   DAOMXN010000084 DAOMXN010000000
VERSION     DAOMXN010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 9479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9479
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3818_length_9479_cov_11.130730"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4473)
                     /locus_tag="PK812_07550"
     CDS             complement(<1..4473)
                     /locus_tag="PK812_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HRJ69448.1"
                     /translation="MARSADKGRSSAEALLAASFESLLFGRVVSEDLVACPPDSLSAI
                     AASAFDRFVRRRPGEVDVRLVDVTLADGRAITIVETVNDDMPFLLDSTLAELGERGLD
                     IHLVAHPILAVKRDAKGALVALQGIAGAAETGDFLRESLIHIHVGAVLDAGQRAGIVA
                     ALERIHQDVHAAVRDWRAMRERLAAVARRYREAPPLLPADEIAEAVQFLDWLLAENFT
                     FLGLREYRFPSLDDASEPVAGEGLGILADPEVKVLRRGAEMVVMTAEVRAFLKEKSAL
                     IIAKANVKSRVHRRVHMDYVGVKLIGPLGQVEGELRLIGLFTSTAYTSSTRVIPYIRH
                     KVAMVMARAGFDPASHSGKALANVLENYPRDELFQLDRETLYGFAMDALSLIERPRIR
                     AMIRVDRFDRFVSVIVYVPKDRYDTAVRTRIGNYLAGRFEGRISAAYPSYPEGPLART
                     HYIIGRYEGQTPLVNRADVEEAIAEICRTWPDKLRRTLAEGERPERAVHLFARYGEAF
                     GAAYREAFTPSESLKDMQILESLRLEAPVAVDFYRKGGQSGAVASLKFFALGAPMPLS
                     QRVPMLENLGFAVNNERTYRVAGRDGGAERTAFLHDMTIERARGGDIDVQTLDTRVED
                     TLMALVRGEAESDRFNALVVDAGLTWRQALLLRAYARYLQQIGAPFGQEYIAGVLGRY
                     PDIAGSLVALFEARFDPAAGATPEAGTALTGEIGARLDGLFEAVSSLDDDRIVRRTRN
                     LIEATLRTNFYQTPDGGHRPTLAFKFEARRVEGLPLPRPLYEIFVHSARVDGVHMRFG
                     KVARGGLRWSDRPMDFRTEVLGLVKAQQVKNAVIVPVGAKGGFFPKRLPPASERDAWF
                     KEGTEAYKIFIGALLDLTDTLDGDAVRPPPQTVRHDGDDPYLVVAADKGTATFSDTAN
                     AISLARGHWLGDAFASGGSVGYDHKKMGITARGAWECVKRHFREIDADIQTQPFTVAG
                     VGDMSGDVFGNGMLLSPAIRLVAAFDHRDIFIDPDPDPTLALAERQRLFALPRSSWQD
                     YDRAKISRGGGVFSRSAKEIVLSEEARRAIGLDQAKATPQEVLSAILKAPVDLLWFGG
                     IGTYIRASDETDAQVGDRANDAIRIPAVALRCKVVGEGANLGCTQRGRIEAARRGIRL
                     NTDAIDNSAGVNTSDVEVNLKIALSRPLSDGRLSEGDRNALLASLTDEVARIVLRNNY
                     LQSLALTLAERQGAIGLTQLQRLMQGLEARGRLDRSVEVLPADPEIARRLGAGEGATR
                     PELAVLLAYAKLSAFEDLIASKVPDDPYFADELDRYFPDEIRRRFPDAVTEHRLRREI
                     IATQLSNIVVNRCGPGAMARFADETGADVPTIALAYAATRDSFGLLDLNGAIDLLDAR
                     LPGAVQVALYAAVQDVLVGRMAWFLRNTDLTSGLGPLVARFRQGVEAARLAVATALPA
                     EFARERDRHGFRPQINSWMVHDAAFSRRCAEAGFVGMTLSAEYGGRGRTPLERHI"
     gene            4633..5226
                     /locus_tag="PK812_07555"
     CDS             4633..5226
                     /locus_tag="PK812_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931028.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HRJ69449.1"
                     /translation="MASTPAGGKHEGHDVMALDLPRGALSPQMEAYFQKCRDKIGFVP
                     NVLQAYAFDTAKLEAFAAFYNDLMLAPSGLSKLEREMIAVAVSSENKCFYCLTAHGAA
                     VRALSGDPALGEMLVMNYRSAPLDARRRAMLDFAVKITTASHAIGEADRAALRATGFS
                     DRDIWDIAAVAAFYNMSNRMASAVDMRPNPEYHAQSR"
     gene            5541..5909
                     /locus_tag="PK812_07560"
     CDS             5541..5909
                     /locus_tag="PK812_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017485361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2794 domain-containing protein"
                     /protein_id="HRJ69450.1"
                     /translation="MSEAESGAGRGDAVVVPFSSQRPPDRVAFSKDELQRILAVYGRK
                     VAQGEWRDYAMEFGRHSAVFSVFRRTSEVPLYRIEKTPALAARQGAYAVVAASGQVLK
                     RGADLARVLMVLEKPSRVIG"
     gene            complement(5992..6771)
                     /locus_tag="PK812_07565"
     CDS             complement(5992..6771)
                     /locus_tag="PK812_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010657904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HRJ69451.1"
                     /translation="MSNFERNAYVPYGGTVATQAQIDQGLRSYMLGVYNHMVLGLAIT
                     GLAALGFYMLSVTTDQAMAVGKLKNGTMLTQFGAMLYTSPLKWVVMFAPLAVVFFFSF
                     RIQSMSAAAARGTFWLYAALVGVSLTTVFFVFTHTSIVRVFFITAASFGALSLFGYTT
                     KKDLSGMGSFLIMGLIGLMIASIVNIFLGSTGLQWAISMLGVLIFAGLTAYDTQNIKN
                     MYYESMGSEMAEKLSVYGALQLYMDFLGMFQMLLSLLGDRE"
     gene            complement(6971..>9479)
                     /locus_tag="PK812_07570"
     CDS             complement(6971..>9479)
                     /locus_tag="PK812_07570"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRJ69452.1"
                     /translation="RDLRGGLSGFGVLIACIAIGVAAIVAVSSLARGLSDGLAREGRV
                     ILGGDIGFSLLQREASEAEMAYINRLGRVQTTAFLRAMTRAEDGGATLAELKAVGPAY
                     PALGVLEVRPQATARDLLAARDGVFGLLADPLLTGRLGLKVGDRLVVGSHAFEIRAEI
                     VREPDRVATGVGFGPRIIVSHDGLRASGLLQPGSLVRWTYRVTLDGDSTSQAQLKRVV
                     EQARAAFPDAGWDIRTRENAAPQLTRNLERFTQFLTLVGLTALAVGGVGVANAAQAFT
                     ERKRGAMAVLKSVGAEGRLIFKLLLIEVLCLGAIGTAIGVVVGALAPFLVKALAGALL
                     PFPLVTSIYVHEIAVGFAYGLLTVAAFSIWPLGRAHDIPVSALFRDLVTGDRRWPRWG
                     YLVLVALTTGLLVAVAVLFAWDRTIALIYVAAAASAFLLLRLVALAIMALARRMPSPK
                     RTELRLALVNMHKPGALTPSVVLSLGLGVTLLVAMGLIDHNLRNQLSRTLPEKAPSFF
                     FLDIPAAETTAFEAMVAGLAPGSRLERVPMMRGRVTALKGVRSEQIKAHERAAWVLEG
                     DRGITFSESPPAGSVLVQGQWWEKDYRGPPLVSFDHELAGWLGLTIGDEITVNVLGRT
                     MTARIANLRKVEWQSLGINFVLVFSPNTFAGAPYSNLATLTFPKGATEAAEIALLKAA
                     ARQFPAVSSVRVKEALETIDTIVAQLAVAVRGASAVAILASVIVLGGALAAGRRVRAQ
                     ESVILKTLGATRRRLLAAMIVEYALLGVAASLFGLAAGALAGYAIVTQVMKLAFSFPW
                     LSMLELTITALSLVIILGLAGTWRILGQKPAPFLRNA"
BASE COUNT         1684 a         3195 c         3161 g         1439 t
ORIGIN      
        1 gatgtggcgc tcgagcgggg tgcggccccg gccgccatat tcggcggaca gcgtcatgcc
       61 cacgaagccc gcctcggcgc agcggcggct gaaggcggcg tcatgcacca tccaggaatt
      121 gatctgcggc cggaagccgt gccggtcgcg ctcgcgcgcg aattccgccg gcagcgccgt
      181 cgccaccgca agtcgcgccg cttcgacgcc ttggcggaac ctcgcgacca acggccccag
      241 tccggaagtc aggtccgtgt tgcgcaggaa ccaggccatg cggccgacca gcacatcctg
      301 cacggcggca tagagcgcga cctggaccgc gcccggcagg cgtgcatcga gcagatcgat
      361 cgccccgttc agatcgagca ggccgaagga atcgcgcgtc gcggcatagg ccagggcgat
      421 ggtcgggaca tccgcgccgg tttcgtcggc gaagcgcgcc atcgcgcccg gtccgcagcg
      481 gttgacgacg atgttggaca gttgcgtcgc aatgatctcg cggcgcagcc ggtgttccgt
      541 caccgcatcg ggaaaacgcc ggcggatctc atccgggaaa tagcggtcca gttcgtcggc
      601 gaaataggga tcgtccggga ctttcgaggc gatcaggtcc tcgaaggccg agagcttggc
      661 ataggccagc aagacggcga gctcgggccg ggtcgcgcct tctcctgcgc cgagccggcg
      721 cgcgatctcc gggtcggcgg ggagcacctc aacgctccgg tcgaggcgtc cgcgcgcctc
      781 cagcccctgc atcaggcgct gaagctgagt caggccgatg gcgccctgcc gctccgccag
      841 ggtcagcgcc agcgactgca ggtaattgtt gcgcagcacg atccgcgcca cctcgtcggt
      901 cagcgaggcc agcagcgcat tgcggtcgcc ctcgctcaga cggccgtccg acaggggtcg
      961 tgacagggca atcttgagat tgacctcgac gtccgaggta ttgaccccgg cggaattgtc
     1021 gatggcgtcc gtgttgaggc ggataccccg ccgcgccgcc tcgatgcgcc cgcgctgggt
     1081 gcagccgagg ttggcgccct cgccgacgac cttgcagcgc agcgcgaccg ccggaatgcg
     1141 gatcgcgtcg ttcgcgcggt ccccgacctg ggcgtccgtc tcgtccgacg cgcggatgta
     1201 cgtgccgatg ccgccgaacc acagcaggtc gaccggtgcc ttcaagatgg cggacagcac
     1261 ctcctgaggc gtggccttgg cctggtccag gccaatggcg cgccgggctt cttccgacag
     1321 gacgatctcc ttggccgaac gggaaaacac acccccgccc cttgagatct tggccctgtc
     1381 ataatcctgc cagctcgacc ggggcagggc gaacaggcgc tggcgctcgg caagcgcgag
     1441 cgtcgggtcg ggatcggggt cgatgaagat gtctcggtgg tcgaaggccg cgaccagccg
     1501 gatggccggc gacagcagca tgccattgcc gaacacatcg ccggacatgt cgccgacacc
     1561 ggccaccgtg aacggttgcg tctggatgtc ggcatcgatc tcgcggaagt gtcgcttgac
     1621 gcattcccag gcgccgcgcg cggtgattcc catcttcttg tgatcgtagc cgacggagcc
     1681 gccggaggcg aaggcgtcgc ccagccagtg cccgcgcgcc agcgaaatcg cgttggcggt
     1741 gtccgagaat gtcgccgtgc ccttgtccgc cgccacgacc agataggggt catcgccgtc
     1801 atggcgcacg gtttgcggcg gcggacgcac ggcatccccg tcgagcgtgt cggtgaggtc
     1861 cagcagggcg ccgatgaaga tcttgtaggc ctcggtgcct tccttgaacc aggcgtcacg
     1921 ctcgctggcg ggcggcaacc gtttcgggaa aaatccgccc ttggcgccga ccggaacgat
     1981 gacggcgttc ttgacctgct gcgccttgac cagaccaagg acctccgtgc ggaagtccat
     2041 ggggcggtcc gaccagcgca ggccgccgcg cgccaccttg ccgaagcgca tgtgcacgcc
     2101 gtccacgcgc gccgaatgca cgaagatttc gtagagcggc cttggcagcg gcaggccctc
     2161 gacgcggcgc gcttcgaact tgaaggccag cgtgggccgg tggccgccat ccggcgtctg
     2221 gtagaagttg gtgcgcagcg tcgcctcgat caggttccgg gtgcgccgca cgatccggtc
     2281 gtcgtcgagg ctcgacacgg cctcgaacag cccgtcgagc cgcgcgccga tctcgccggt
     2341 cagggccgtc ccggcctccg gcgttgcccc ggcggccgga tcgaaccggg cctcgaacag
     2401 cgcgacaagg ctgccggcga tgtcgggata gcggccgagc acgccggcga tgtactcctg
     2461 cccgaagggc gcgccgatct gctgcaggta gcgggcatag gcgcgcagca gcagcgcctg
     2521 ccgccaagtc agccccgcat ccaccaccag ggcattgaag cggtcggatt ccgcctcgcc
     2581 ccgcaccagc gccatcagcg tgtcctcgac gcgcgtgtcg agcgtctgca cgtcgatatc
     2641 gccgccgcgg gcacgctcga tcgtcatgtc gtgcaggaag gccgtgcgct ccgctccgcc
     2701 atcgcggccg gcgacgcggt aggtgcgctc gttgttgacg gcgaaaccga ggttctcgag
     2761 catcggaaca cgctgcgaca acggcatcgg cgcgcccagg gcaaagaact tcaggctcgc
     2821 caccgcgccg gactgcccgc ccttgcggta gaagtccacg gcgacgggag cctccagtcg
     2881 cagcgattcc agaatctgca tgtccttgag cgattccgac ggcgtgaagg cctcccgata
     2941 ggcggcaccg aacgcttcgc catagcgcgc gaacagatgc accgcccgct ccggcctctc
     3001 gccctcggcc agcgtgcgcc gcagcttgtc cggccatgtc cggcagatct cggcgatcgc
     3061 ttcctcgaca tcggcgcggt tgaccagcgg cgtctgaccc tcgtagcgcc cgatgatgta
     3121 gtgggtgcgt gcgagcgggc cttccggata ggacggatag gcggccgaga tgcgcccctc
     3181 gaagcgaccg gcgagataat ttccgatccg cgtgcgcact gcggtgtcgt agcggtcctt
     3241 cggcacatag acgatgaccg agacgaaacg gtcaaagcgg tcgacgcgga tcatggcccg
     3301 gatgcgcggc cgctcgatca gcgagagcgc atccatggcg aacccgtaaa gggtttcccg
     3361 gtcgagctgg aacaactcgt cgcgcgggta gttctccagg acgttcgcca gcgccttgcc
     3421 ggagtggctc gccggatcga aaccggcccg cgccatcacc atcgccacct tgtggcggat
     3481 gtaggggatg acgcgcgtcg aagaggtgta ggccgtggag gtgaacagtc cgatcagcct
     3541 gagctcgcct tccacctggc cgaggggacc gatcagcttc acgccgacgt aatccatgtg
     3601 cacgcgccgg tgcacccgcg atttcacatt ggcctttgcg atgatgagcg ctgacttttc
     3661 cttcaggaaa gcgcgcactt ccgcggtcat caccaccatc tcggcgccgc gccggagcac
     3721 cttcacctcc gggtcggcca ggatgccgag accctcgcct gcgaccggct cgctggcgtc
     3781 gtccagagag gggaagcgat actccctgag gccgaggaag gtgaaattct cggcaagcag
     3841 ccagtcgagg aactggaccg cctccgcgat ctcgtcggcc gggagcaacg ggggcgcctc
     3901 gcggtagcgg cgcgccacgg cggcaagacg ttcacgcatc gcgcgccagt cgcgcacggc
     3961 ggcgtgcacg tcctggtgga tacgttcgag cgccgcgacg ataccggccc gctggcccgc
     4021 gtccagcacc gccccgacat ggatgtggat caggctctcg cggagaaagt cgccggtttc
     4081 cgccgctccg gcgatcccct ggagggcgac gagcgctccc ttcgcatcgc gcttcaccgc
     4141 gaggataggg tgggcgacca ggtggatgtc gaggccgcgc tcgccgagtt ccgcgagggt
     4201 ggaatcgagc aggaacggca tgtcgtcgtt cacggtctcg acgatggtga tggcccgtcc
     4261 gtcggcgagc gtcacatcca ccagccggac atcaacctcg ccgggccggc ggcgcacgaa
     4321 ccggtcgaaa gcggatgccg cgatcgccga cagggaatcg ggcggacagg cgaccaggtc
     4381 ctcggagacg acgcgcccga acagaagcga ttcgaacgag gccgcgagca gcgcctccgc
     4441 cgatgatctt cccttgtccg cagacctggc catgacgctc cccgactttg attatccaaa
     4501 agtctaagca cgatttggcg cgcaagctca atggcatcac ccgcaggaag cggaagttgg
     4561 cgctggactg gtcttgtcac cggcgaagtt ccgcgaaaga cttgcgcggc acgaacggaa
     4621 gggaacgcac cgatggcttc gacacctgca ggcgggaaac acgaggggca cgacgtgatg
     4681 gcgcttgacc tgcccagggg tgccctctcg ccgcagatgg aagcctattt ccagaagtgc
     4741 cgcgacaaga tcggctttgt ccccaacgtg ctgcaggcct atgccttcga cacggccaag
     4801 ctcgaggcct tcgccgcctt ctacaacgac ctgatgctgg ctccgtccgg cctgtcgaag
     4861 ctggaacgcg agatgatcgc cgtcgcggta tcctcggaga acaagtgctt ctactgcctc
     4921 acggcccatg gcgcggccgt gcgcgcgctc tccggcgatc cggcgctggg ggaaatgctg
     4981 gtgatgaact accgctccgc gccgctcgac gcccgccggc gcgccatgct ggacttcgcg
     5041 gtcaagatca cgactgcatc ccatgccatc ggcgaagcag atcgcgcggc cttgcgcgcc
     5101 accggctttt ccgatcgcga catctgggac atcgcagccg tcgccgcctt ctacaacatg
     5161 tccaaccgca tggcctcggc ggtcgacatg cgcccgaacc ccgaatacca tgcccagtcc
     5221 cgctgaaccg gtcgcttcgg caaagaaaag gcggccgacc cgggcgaggt cggccgccgt
     5281 tgcattggtt ggatttctcc aggatcgccg gggtccgagg ggctgggggg cgtacgacat
     5341 cggacccgaa gacgactttc acctccgcat gggggtgata tcgccgactg ccttggcgcg
     5401 ctcatggttg aaaagcggcg aaaatgcgga atcgttcgca gatgcgtgat cgccggcgcg
     5461 ccagccctca cggaagcgct tgccaagccc ggtcgccggc gcaatgctga ctagcaggac
     5521 aaatcaggat ctggcggggg atgagcgaag cagaatctgg cgccgggcgc ggcgatgcgg
     5581 tcgtggtgcc gttctccagc caacgacccc ccgaccgcgt ggcgtttagc aaggatgaac
     5641 tccagcgcat cctcgctgtc tatggccgca aggtggcgca gggcgaatgg cgggactacg
     5701 ccatggaatt tggccgccat tcggcggtgt tcagcgtttt ccgccgcacc tcggaggtcc
     5761 cattgtaccg catcgagaag accccggcgc tggctgccag gcagggcgcc tatgcggtcg
     5821 tggcggcgtc gggccaggtc ctgaagcgcg gcgcggacct tgcgcgcgtg ctgatggtgc
     5881 tggagaaacc ctcgcgggtg atcggctagc cggcgtcggc cttgggccgg aaacgaaaac
     5941 cccggcgttt ccgccggggt tccgtgaagc ctttgcgctt cgagacgcgt cttactcgcg
     6001 atcgccgagc agcgacagca gcatctggaa catgcccagg aagtccatgt agagctggag
     6061 agcgccgtag accgagagct tctcggccat ctccgagccc atcgactcat agtacatgtt
     6121 cttgatgttc tgcgtgtcgt aggcggtcag gcccgcgaag atcagcacgc ccagcatcga
     6181 gatcgcccac tgcaacccgg tcgagcccag gaagatgttg acgatgctcg cgatcatcag
     6241 gccgatcaga cccatgatca ggaacgagcc catgccggac agatccttct tcgtggtgta
     6301 gccaaaaagg ctcagcgcgc cgaacgaggc cgccgtgatg aagaacacgc gcacgatcga
     6361 ggtgtgcgtg aacacgaaga acacggtggt gagcgacacg ccaaccagcg ccgcgtacag
     6421 ccagaacgtg ccgcgggcgg cggcggcgct catcgactgg atgcggaacg agaagaagaa
     6481 caccaccgcg agcggggcga acatcaccac ccatttcagc gggctggtgt acagcatcgc
     6541 gccgaactgg gtgagcatcg ttccgttctt gagcttgccc acagccatgg cctgatcggt
     6601 cgtgaccgaa agcatgtaga agcccagggc cgcgaggccg gtgatggcaa ggcccagcac
     6661 catgtgattg tagacgccga gcatgtagga gcgcagaccc tggtcgatct gggcctgggt
     6721 cgccacggtg ccgccgtagg gcacgtaggc gttgcgttcg aagttcgaca tagaaaccct
     6781 ctttgagttc gacccggtgc ggggcagccc cacgcttctc gccggatcaa gccagcataa
     6841 tatggcgaag tcacggcttt cttacaagag tttcgaagag cggtcgcgct gatttcacct
     6901 cgattggttt agaggcgggc ctgcgaaaga tcattcgggg ccggaccgta gaaacacgcg
     6961 gtggcgtttc tcaggcgttg cgcaagaatg gcgcaggctt ttgtccaagg atgcgccacg
     7021 tcccagccaa accgagaata atcaccaacg atagcgcagt aatagtgagc tcgagcatgc
     7081 ttagccacgg aaacgaaaac gcgagcttca tcacctgggt tacaatggcg tagcccgcca
     7141 gcgcgccggc cgccaggccg aacaacgaag cggcgacgcc cagcagggca tactcaacga
     7201 tcatcgcggc caagagcctg cgccgcgtcg cgcccagcgt cttgaggatc accgattcct
     7261 gagcgcgaac gcgccggccc gcggcaaggg cgccaccgag cacgatgacg ctggcgagga
     7321 tggcgacggc gctcgcgccg cgcacggcca ccgccagctg cgcgacgatg gtgtcgatcg
     7381 tctcaagcgc ttccttgacg cgcacgctgg aaaccgccgg gaactgacgc gccgccgcct
     7441 tgagcagcgc gatctcggcg gcctcggtcg cgcccttcgg aaaggtgagc gtggcaaggt
     7501 tcgaataggg cgcgccggcg aacgtgttgg gcgagaacac cagcacgaag ttgatgccga
     7561 gcgactgcca ttccaccttg cgcaggttag cgatgcgcgc cgtcatcgtg cgccccagca
     7621 cgttcacggt gatctcgtcg ccgatggtca gaccgagcca gcccgccagc tcgtgatcga
     7681 aggacacaag cggcgggccg cggtagtcct tttcccacca ctgcccctgc accagcaccg
     7741 acccggcagg cgggctttcg gagaaggtga tgccgcggtc gccttccagc acccaggcgg
     7801 cgcgttcatg cgccttgatc tgttcggagc gcacgccctt cagcgccgtg acgcggcctc
     7861 gcatcatcgg cacgcgttcg agcctgctgc ccggcgccag gccagcgacc atcgcctcga
     7921 acgccgtcgt ctccgccgcc ggaatatcga ggaaaaagaa gctcggcgcc ttttccggca
     7981 gtgtccgcga cagctgattg cgcaggttgt gatcgatcag gcccatggcg accagcagtg
     8041 tcacgccgag gccgagcgac agcaccaccg acggcgtcag ggcgccgggc ttgtgcatgt
     8101 tgaccagcgc cagccgcagt tccgtgcgtt tcggcgaggg catccgccgc gccagcgcca
     8161 tgatggcaag cgcgacaagc cgcaacagca ggaaggcgga cgccgcggcc gccacataga
     8221 tcagcgcgat ggtgcggtcc caggcgaaca gcaccgccac ggcgaccagc aatcctgtcg
     8281 tcagcgccac aagcaccagg tagccccagc gcggccagcg ccggtcgccc gtgaccaggt
     8341 cgcggaacag cgccgagacc ggaatgtcgt gcgcccggcc aaggggccag atcgagaacg
     8401 cggcgaccgt aagcaacccg taggcaaagc cgacggcgat ctcgtggaca tagatcgacg
     8461 tcaccagcgg gaagggcagc agcgcgccgg ccagcgcctt caccaggaag ggcgccaggg
     8521 cgccaacgac aacgccgatg gccgtgccga tggcgccgag gcacaggacc tcgatcagca
     8581 gcaacttgaa aatgagccgg ccttcggcgc ccacgctctt caataccgcc attgccccgc
     8641 gcttgcgctc ggtgaaggcc tgcgcggcgt tggccacccc gacgccgccg accgccagcg
     8701 ccgtcagtcc gaccagcgtc aggaactgcg tgaaacgttc gagattgcgc gtcagttgcg
     8761 gcgcggcgtt ctcgcgggtg cggatatccc agccggcgtc cggaaaggcg gcacgggcct
     8821 gctcgaccac ccgtttcagc tgcgcctgcg aggtggaatc gccgtcgagc gtgacgcgat
     8881 aggtccagcg caccaggctg ccgggttgca gcagaccgct ggcgcgcagc ccgtcgtggg
     8941 aaacgatgat gcgcggcccg aacccgaccc ccgtggcgac acgatctggc tcgcgcacga
     9001 tctcggcccg gatctcgaaa gcatggctgc cgacgaccag ccggtcgccg accttcagtc
     9061 cgagccgtcc ggtcagcagc ggatcggcca gcaggccgaa cacgccgtcg cgggcggcga
     9121 gaagatcgcg cgccgtggcc tgcggacgga cctcgagcac tccaagagcg ggataggcag
     9181 gtcccacggc cttcagttcg gcgagtgttg cgccgccatc ctcggcacgc gtcatggccc
     9241 gcaggaaggc cgttgtctgc acccgcccga gccggttgat ataggccatt tcggcctcgc
     9301 tggcttcgcg ctgcagcaac gagaagccga tgtcgccccc gagaatcacg cgcccctcgc
     9361 gggccaggcc gtccgacagt ccgcgcgcca gcgaggacac cgcgacgatc gccgcgacgc
     9421 cgatggcgat acaggcgatc agcacgccga aaccgctcag gcccccgcgc agatcgcgc
//
LOCUS       DAOMXN010000085         9475 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_3821_length_9475_cov_11.011146, whole genome shotgun sequence.
ACCESSION   DAOMXN010000085 DAOMXN010000000
VERSION     DAOMXN010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 9475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9475
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3821_length_9475_cov_11.011146"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="PK812_07575"
     CDS             complement(<1..262)
                     /locus_tag="PK812_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRJ69453.1"
                     /translation="MTTGNEAPLIALKAVSKRFVRPVDVAGKIANMLGADNREITVHA
                     VDRVDLSVAAGEVVGLVGESGCGKSTVGRVVAGVHAATSGQVL"
     gene            complement(259..1242)
                     /locus_tag="PK812_07580"
     CDS             complement(259..1242)
                     /locus_tag="PK812_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007764498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ69454.1"
                     /translation="MSPVLEVTGLRTHFFTRGGVVKSVDGVSLSLNAGEVLGLVGESG
                     SGKSVTGFSILGLVDPPGRIVEGSIRLAGRELVGLEEGELQSVRGRDLAMIFQDPMMT
                     LNPVLKVGTQMIETVLAHEKVSEKSARARCIDALSMMGIPAPHERLDAYPHQFSGGMR
                     QRVAIAIALLHNPQVVIADEPTTALDVTIQSQILAKVQELCRERGTALIWITHDLTVI
                     AGLADRIAVMYAGRIVEEGGVDAVLDRPRHPYTQGLIGSVPSRGRKGEKLAQISGSAP
                     SLANLPVGCAFAPRCDKAINRCRVEAPVLPNAAAGSRAACHLAVPTAGQAA"
     gene            complement(1245..2165)
                     /locus_tag="PK812_07585"
     CDS             complement(1245..2165)
                     /locus_tag="PK812_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ69455.1"
                     /translation="MSSKAAPVPAAAPAAQEETPFRRFVAQFAEDRIAVAGLLVFTLI
                     VLAALLAPWITPQNPYSLAKLDLMDSRLPPGSKAGDGFTMMLGSDGFGRDIFSAIFYG
                     LRISLAVGAISCLIAATVGMTVGLIAAYAGGRVENLIMRLVDLQLSLPAILLALILVS
                     MFGKGVDKIIIALVLVQWAYYARTVRGSALVERGKDYVEAARCLGLSHARIVFRHILP
                     NCLAPIIVVATVQTAHSISLEATLSFLGVGLAQTEPSLGVLISNGFQYMLSGKYWISF
                     FPGLALLVTIVSINLVGDRLRDVLNPRLEK"
     gene            complement(2162..3139)
                     /locus_tag="PK812_07590"
     CDS             complement(2162..3139)
                     /locus_tag="PK812_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008550673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ69456.1"
                     /translation="MLAFILRRAGQGLLVILAMVVIVFFGVNVVGDPVWILVSPDMTQ
                     DQIAEVSRSLGLDRPIWEQFFHFVGGILQGDLGRSFVHGTPAIRLILDRMPATIELAL
                     VALFLAVILGIPLGLFAGLKPDHPLSRGVMAVSILGFSLPTFWVGLMLIMVFAVMLGW
                     LPATGRGDLGSFFGITTSLVTLDGWSHILMPAFNLALLKISLVIRLARAGTREVSLQD
                     YIKFARAKGLSRSRIIGVHVLKNILIPIVTVLGLEFGSLVAFSVVTETIFAWPGMGKL
                     LLEAIQRLDRPVIVAYVIIVVVLFVVINLIVDILYSLLDPRVRLSGAPA"
     gene            complement(3187..4764)
                     /locus_tag="PK812_07595"
     CDS             complement(3187..4764)
                     /locus_tag="PK812_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRJ69457.1"
                     /translation="MKLAMKRALLATCAVAYATFALAQDIRIGTSSEPTSMDPHFHNL
                     TPNNQIMAHIFEPLISMDAKQTIRPGLAESWKVIDDTTWEFKLRAGVKFHDGSDFGVD
                     DVIATWARAPEVPKSPSNFSAYIRGKTIEKVDDLTFRIKTAGPEPLMLNHLAQFGIIS
                     RKNKDMTTEDYNSGKAASGTGPFKFSEYVAGDRVVLTRNDTHWGGKPTWGKVTFKSIK
                     SDPTRVAALLSGDVDVIENVPTADAQRIKKDARLTTASALSNRVIYFHLDRHRDTTPF
                     IKGKDGADIKNPLYDLRVRQALSKALNRPAIVERVMEGEAEPASQFIPESYAGTSKNL
                     KPTAYDLDGAKKLLAEAGLPNGFKITIHGPNGRYTNDAKILEAAGQMWARLGLDVSVE
                     AIPAANFFSRASTGGAGGVPEFSLFLVGWSAGTGEPSDSVKALTTTFDRSKGTGSANR
                     GRYSNPKVDELLLAAIRTVDNAKRNAMLAEATEIAMNDVAIIPIHYPLNTWAAKKEFT
                     ITPRSDEYTLATSFGRK"
     gene            4901..5635
                     /locus_tag="PK812_07600"
     CDS             4901..5635
                     /locus_tag="PK812_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012318072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HRJ69458.1"
                     /translation="MRIRAPLLLVMVSLGAMDARADPCAARVLQGRNVVVCAVDLSRQ
                     SVRLLWKNERGEPYGTLRGIAGAASQQGKPLLFAMNAGMYHHDLSPVGLYVENGVQHA
                     PLNTAGGPGNFHMRPNGVFYVAGGTAGVMESRAYQTRRQKVDFATQSGPMLVIGGKLH
                     PRITGEGTSRKIRNGVGVRDGRTAFFVISDEAVTFTEFAKMFRDDLKTPNALYFDGSI
                     SSLYAPSVNRADGFMPVGPIVAAFGR"
     gene            complement(5654..>8008)
                     /gene="fdhF"
                     /locus_tag="PK812_07605"
     CDS             complement(5654..>8008)
                     /gene="fdhF"
                     /locus_tag="PK812_07605"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009758437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HRJ69459.1"
                     /translation="LPLHDASHPAIAVNLDACIACGLCERACREVQVNDVIGMGRRGH
                     ETRPIFDLADSMGDSTCVACGECVQACPTGALYEKALMDPAARRRAVVPERWVDTLCP
                     YCGVGCQTRVGVKDNRIVKVDGRDGPANDSRLCVKGRFGFDYIHHPHRLTKPLIRRDD
                     APKRGEATIDPANPWDTFREASWEEALARAASGLTAIAAEHGGGAIAGFGSAKGSNEE
                     AYLFQKLIRQGFGTNNVDHCTRLCHASSVAALLEGIGSGAVSAPFRAAEDADCIIVIG
                     ARPTENHPVAATWFKQAAKRGATLIVMDPRGNPLFRHATHAVRFRPGADVALLNGMLQ
                     SIVEEGLINRDFIAEHVAFYEDFSRAIAPYTPEAMAPLCGIDAQTIRTLARTYARAEK
                     SIIFWGMGISQHTHGTDNARCLIALALITGHIGRPGTGLHPLRGQNNVQGASDAGLIP
                     MMFPDYHLVEKEEFRLAFETAWGRPLDPKRGLTVVETINAAYDGRVRGMLIMGENPAM
                     SDPDQSHARAALAKLKHLVVQDIFLTETAWHADVVLPASAHPEKLGTFTNTNRQVQLA
                     LPAVPPPEGARQDIEILHDLASRIGLNWPPCDVAAVYEEMRSHMPSLAGVPWTRLVRE
                     GAVTYPADDALSPGHDIIFSDRFPTPSGRGKLVPAHPLPPDERPDAAYPLVLTTGRLL
                     EHWHTGSMSRRSDLLDTLECGAIAVMNPQEIQRSGHQSGDRIALETRRGRIEALLRSD
                     PAMPEGMVFVPFAFNEGPANRLTNAALDPFGKIPELKYCAARIV"
     assembly_gap    8009..8108
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8109..8411)
                     /locus_tag="PK812_07610"
     CDS             complement(<8109..8411)
                     /locus_tag="PK812_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009466765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRJ69460.1"
                     /translation="MSATIRFTLDGRPVEAGPEETLWQVARREGVEIPHLCHCEQKGY
                     RPDGNCRACVVEIAGERTLAASCIRKPADGMAVKTGGERVEKARRMVFELLEADMPA"
     gene            complement(8408..>9475)
                     /locus_tag="PK812_07615"
     CDS             complement(8408..>9475)
                     /locus_tag="PK812_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HRJ69461.1"
                     /translation="EDLIALLSDAGLRGLGGAGFPAGRKWSIVRAQPGAKLMTINADE
                     GEPGTFKDRVLLESDPHRMLEGALIAAHVVSAERIYIYLRDEYPGVRAILLREIAALE
                     QAGLLTTPVELRRGAGAYVCGEESAMLESLEGKRGLPRHRPPYIAEKGLFGRPTLNHN
                     VETLFWVRDIAEKGAEWFARQGRRGAKGVRSYSVSGRVRKPGVKLAPAGITLNELVAE
                     FCGGMAEGHRLAGYLPGGASGGILPAALADEPLDFGTLEKHGCFVGSHAIVVFSDRDD
                     PRAIARNLMAFFAHESCGQCTPCRAGTEKLGALMQAGTWDEALMADIEQVMRDASICG
                     LGQAAPNPMRSILKFFAGSQP"
BASE COUNT         1652 a         2956 c         3198 g         1569 t
ORIGIN      
        1 agaggacctg gcccgaggtg gcggcgtgca cgccggcgac gacgcgcccg acggtagact
       61 tgccgcagcc agactcgccc accagcccga ccacctcgcc ggcggcaacg gacaggtcaa
      121 cgcggtcgac ggcatgcacc gtgatttccc ggttgtccgc gcccagcatg ttggcgatct
      181 tgccagcgac gtcgacgggc cggacgaagc gcttcgatac ggccttgagt gcgatcagcg
      241 gggcttcgtt gccggtcgtc atgccgcttg ccccgctgtc ggaacggcca ggtggcaggc
      301 agcgcggctt ccggccgcgg cgttcggcag gaccggcgct tcaacgcggc agcgattgat
      361 cgccttgtcg cagcgcggcg cgaaggcgca accgaccggc aggttcgcca gggaaggggc
      421 agagccggag atctgcgcca gtttctcccc cttgcggccg cggctgggaa ccgagccgat
      481 caacccttgc gtatagggat gacgcggccg gtccagcacg gcgtcgacgc caccttcctc
      541 gacgatccgg cccgcgtaca tcaccgcgat ccggtcggcg agcccggcga tgacggtgag
      601 gtcatgggtg atccagatca gcgccgtgcc gcgctctctg caaagctcct gcacttttgc
      661 gagtatctgg ctttgaatcg tcacgtcgag ggccgtggtc ggctcatcgg cgatgacaac
      721 ctgcggattg tgcaggagcg cgatggcgat ggcgacgcgc tggcgcatgc cgccggaaaa
      781 ctggtgcgga taggcgtcca gccgttcgtg cggcgcggga atgcccatca tcgacagcgc
      841 atcgatgcag cgcgcgcggg ctgacttctc ggaaaccttc tcgtgcgcga gcacggtctc
      901 gatcatctgc gtgccgacct tgaggaccgg attgagcgtc atcatcgggt cctggaagat
      961 catggcgagg tctcgcccgc gcaccgactg cagctcgcct tcctcaaggc ccaccagctc
     1021 gcgcccggcg agccggatcg aaccctcgac gatccgtccg ggcgggtcga caagtccgag
     1081 gatggagaag ccggtcacgc tctttccaga gccggattct ccgacaagtc cgagcacttc
     1141 accggcgttc agcgacaggc tcaccccatc caccgacttc acgacgccgc cgcgcgtgaa
     1201 gaaatgcgtt ctcaggccgg tgacttccag aacgggcgac atgctcattt ctccagccgg
     1261 ggattgagca cgtcgcgcag gcggtcgccg acgagattga tcgacacgat ggtgaccagc
     1321 agcgccaggc cggggaagaa gctgatccag tattttcccg acagcatgta ctggaagccg
     1381 ttggagatca gcacgccgag cgacggctct gtctgggcca gtccgacgcc cagaaacgag
     1441 agcgtggctt cgagggagat cgaatgcgcg gtctgcacgg tggcaacgac gatgatcggc
     1501 gcgagacagt tcggcaggat atggcggaac acgatgcggg catgcgacag gcccaggcag
     1561 cgcgcggctt ccacgtaatc cttgccgcgc tcgaccagcg ccgaaccgcg cacggtgcgt
     1621 gcgtagtacg cccattgcac caggacgagg gcgatgatga tcttgtccac gcccttgccg
     1681 aacatcgaca cgaggatcaa agccaacaga atcgccggca gcgagagttg cagatctacc
     1741 aggcgcatga tcaggttctc gacccggccg ccggcatagg cggcgatcag gccgaccgtc
     1801 atgccgacag tggctgcgat caggcagctg atggcgccaa ccgccagaga gatgcgcagg
     1861 ccatagaaaa tcgcggaaaa gatatcacgg ccgaacccgt cggagccgag catcatggtg
     1921 aagccgtcgc ccgcctttga gccgggcggc aggcgcgagt ccataaggtc gagcttggcc
     1981 aggctgtagg ggttttgcgg ggtgatccag ggcgccaaca gcgccgcgag cacgatcagc
     2041 gtgaaaacca ggagaccggc gacggcgatg cggtcttcgg cgaactgggc gacaaaacgg
     2101 cgaaagggcg tttcttcctg cgccgcgggc gccgcagcgg gaacgggagc agccttggaa
     2161 ctcatgccgg tgctcccgac aggcgcacgc gtgggtcgag cagcgaatag aggatgtcga
     2221 cgatcaggtt gatcaccacg aacagcacga ccacgatgat cacgtaggcc acgatcaccg
     2281 gccggtcgag gcgctggatc gcctccagca gcagcttgcc catgccgggc caggcaaaga
     2341 tcgtctcggt caccacggag aaggcgacga gcgagccgaa ttcgagaccg agcaccgtga
     2401 cgatcggaat gaggatgttc ttcagaacgt gcacgccgat gatgcgggag cggctgaggc
     2461 ccttggcgcg tgcgaacttg atgtagtcct gcagggacac ttcccgggtg ccggcgcgcg
     2521 ccagcctgat gacgagtgag atcttgagca gcgccaggtt gaaggccggc atcaggatgt
     2581 gagaccagcc gtccagggta accagcgacg tggtgatgcc gaagaaggac ccgagatcgc
     2641 cccgtcccgt ggccggaagc caacccagca tcaccgcgaa caccatgatc agcatcagcc
     2701 cgacccagaa agttggcagt gaaaagccga ggatcgaaac ggccatgaca ccgcgcgaca
     2761 gcggatgatc gggcttcagg ccagcgaaca gcccgagcgg gatgccgaga atgacggcaa
     2821 ggaacagtgc caccagggcc agttcgatcg tcgccggcat gcggtcgagg atgagccgga
     2881 tggccggggt gccgtggaca aaggaccgcc caagatcgcc ctgcaggatg ccgccgacga
     2941 aatggaagaa ctgctcccag atcggccggt ccagcccgag cgagcgcgac acttccgcga
     3001 tctggtcctg cgtcatgtcg gggctgacaa ggatccagac cggatcgccg acgacattga
     3061 cgccgaagaa cacgatcacg accatcgcca ggatgaccag caggccctga cccgcgcggc
     3121 ggaggatgaa ggcgagcatg ggtacgtcct ttgggagagg ggcgcgcccc gcgcgccccg
     3181 tctacctcat ttcctgccga aggaggtcgc gagcgtgtac tcgtcggagc gcggagtgat
     3241 ggtgaactcc ttcttcgcgg cccaggtgtt caacgggtag tggatcggga tgatcgcgac
     3301 gtcgttcatg gcgatttcgg tcgcctcggc cagcatggcg ttgcgcttgg cgttgtcgac
     3361 ggtacggatc gccgccagca gcagttcatc gaccttcgga ttggagtagc gaccacggtt
     3421 ggccgatccc gtgcccttgg agcggtcgaa agtcgtcgtc agggccttga ccgaatcgga
     3481 tggctcgccc gtgccggcgc tccagccgac gaggaagagc gaaaactccg gcacgccgcc
     3541 ggcgccgccg gtggaggcac gcgagaagaa gttcgccgcc gggatggctt cgaccgacac
     3601 gtcgaggccg agtcgcgccc acatctggcc ggcagcctca aggatcttgg cgtcattggt
     3661 gtagcggccg ttcgggccat gaatggtgat cttgaagccg ttcggcagcc cggcttccgc
     3721 gagcaacttc ttcgcgccat cgagatcgta ggccgtcggc ttcagattct tggaagtacc
     3781 ggcatacgac tcgggaataa actggctcgc cggctcggcc tcgccttcca tgacgcgctc
     3841 gacgatggct ggacggttca gcgccttcga cagcgcctgg cgcacgcgca ggtcgtacag
     3901 cgggttcttg atgtccgcgc cgtccttgcc cttgatgaac ggtgtcgtgt cgcgatgccg
     3961 gtcgagatgg aaatagatca cgcggttgga cagggcgctg gccgtcgtca gcctggcgtc
     4021 cttcttgatg cgctgcgcgt ccgcggtcgg cacgttctcg atcacgtcga catcgcccga
     4081 gagcagcgcg gctacgcggg ttggatcaga cttgatggac ttgaacgtca ccttgcccca
     4141 ggtcggcttg ccgccccaat gggtgtcgtt gcgcgtcagt acaacgcggt cgcccgccac
     4201 gtactcggag aacttgaagg ggccggttcc cgaagccgcc ttgccggaat tgtagtcctc
     4261 cgtggtcatg tccttgttct tgcgggaaat gatcccgaac tgggcgagat ggttcagcat
     4321 cagcggctca ggacccgccg tcttgatgcg gaatgtcagg tcgtcgacct tctcgatggt
     4381 cttgcccctg atataggccg agaaattcga gggactcttc ggcacttccg gtgcccgcgc
     4441 ccaggtggcg atgacatcgt ccacgccaaa atccgagccg tcatggaact tcacgccggc
     4501 gcgcagcttg aactcccagg tcgtgtcgtc gatcaccttc caggactcgg ccaggcccgg
     4561 ccggatcgtc tgcttggcat ccatgctgat caatggctcg aaaatatggg ccatgatctg
     4621 gttgttcggc gtcaggttgt ggaaatgcgg gtccattgag gtcggctcgg acgacgtgcc
     4681 gatgcggatg tcctgtgcca gtgcaaatgt cgcataggcc accgcgcacg tggccagcag
     4741 agcgcgcttc atcgcgagct tcatgatgtt ctcctccaac cgattgtagc ccaccctccc
     4801 ggacggggcc atgtggccgc aatctggaac gccggaaccg cggagacaag cgttttcttg
     4861 cccattgagc gcaggcagtc ccgtcctgta tcaacggacc atgcggattc gcgcgccact
     4921 tctactggtc atggtcagcc tgggtgcgat ggacgcccgc gccgatccct gtgcagcgcg
     4981 ggtcctgcag gggcggaacg tggttgtctg cgcggttgat ctttcgcgcc agagcgtgcg
     5041 gctgttgtgg aagaacgagc gcggcgaacc ctacggcacg ctgcgcggga tcgccggggc
     5101 ggcaagccag cagggcaaac cgttgctgtt cgccatgaat gccggcatgt atcatcacga
     5161 cctgtcgccg gtcgggctct atgtcgaaaa cggcgtgcag catgcgccgc tgaacaccgc
     5221 cggcgggccg ggcaattttc acatgcggcc gaacggcgtg ttctacgtcg cgggcggaac
     5281 cgccggcgtg atggaaagcc gggcctacca gacgcggcgt caaaaggttg attttgctac
     5341 gcaatccgga ccgatgctgg tgatcggcgg aaagctgcac ccccgcatca ccggggaggg
     5401 gacctcgcgc aagatccgca acggggtcgg cgttcgcgac ggccgcacgg cgtttttcgt
     5461 gatcagcgac gaggcggtga ccttcacgga gttcgcgaag atgtttcgcg atgacctgaa
     5521 gacgccgaac gcgctgtatt tcgacgggtc gatctccagc ctctacgccc cgtccgtgaa
     5581 ccgtgcggac ggcttcatgc cggttggccc gatcgtcgcg gcattcggtc gataacgatc
     5641 caacatattg attctaaacg attcttgcgg cgcagtactt cagttccgga atcttgccga
     5701 atgggtccag agccgcgttg gtgagccggt tcgccggacc ctcattgaag gcgaagggga
     5761 caaagaccat accctcaggc atggcggggt cggaacgcag cagcgcctcg atgcggccgc
     5821 gcctcgtttc gagggcgatc cgatctccgg attgatggcc agagcgttga atttcctgcg
     5881 gattcatcac ggcaatcgcg ccacattcca gggtgtcgag cagatccgaa cggcgggaca
     5941 tggacccggt gtgccaatgc tccagcaggc gcccggtcgt gaggaccagc ggatacgccg
     6001 cgtccggacg ctcatcgggc ggcaggggat gcgccggaac cagcttgccg cgaccgctcg
     6061 gcgtcggaaa gcggtcggaa aaaatgatgt catgcccggg acttagcgca tcgtccgccg
     6121 gataggtgac ggcgccttcc cgaaccaggc gcgtccaggg caccccggca agcgagggca
     6181 tgtgcgaccg catctcctca tagacggcag ccacgtcgca cggtggccag ttcaggccga
     6241 ttcggctggc cagatcatga aggatttcaa tgtcttgccg cgctccttcc ggaggcggaa
     6301 cagccggcag cgcgagctgc acctggcggt tggtgttggt gaaggtgccg agcttttccg
     6361 gatgcgccga ggccggcaga accacatcgg cgtgccaggc cgtctcggtc aggaaaatgt
     6421 cctgcacgac cagatgcttg agcttcgcca gcgcagcgcg ggcatggctt tggtccgggt
     6481 ccgacatggc cggattctcg cccatgatca gcatgcccct gacgcggccg tcataggcgg
     6541 cgttgatggt ctcgaccacg gtcagaccgc gcttcggatc gagcggacgg ccccaggcgg
     6601 tctcaaacgc cagcctgaat tcctcttttt ccacaagatg atagtcgggg aacatcatgg
     6661 gaatcaggcc ggcgtccgag gcgccctgca cattgttctg accccgtaac ggatgcaggc
     6721 cggtgccggg ccggcctata tgcccggtga tcagcgccag cgcgatcagg cagcgggcgt
     6781 tgtcggtgcc gtgggtgtgc tgggaaatgc ccatgcccca gaaaattatg gacttttcgg
     6841 cacgggcata ggtgcgcgcg agcgtgcgga tggtctgggc gtcgatgccg cacaggggcg
     6901 ccatggcctc aggcgtgtag ggcgcgatgg cgcgactgaa atcctcgtag aacgcgacat
     6961 gttcggcaat gaaatcccgg ttgatcaggc cttcttcgac aatgctctgg agcatgccgt
     7021 tcaaaagcgc cacgtcggcg cccggccgga agcggacggc atgggtggca tggcggaaca
     7081 gcgggttgcc gcgcgggtcc atgacgatca gcgtcgcgcc gcgcttggcc gcctgcttga
     7141 accaggtggc ggcgacggga tggttctcgg tcggccgcgc gccgatgacg atgatgcagt
     7201 cggcatcctc cgccgcgcgg aagggggcgg acaccgcgcc ggagccgatg ccttccagca
     7261 gggcggcgac cgacgaggca tggcagagcc gcgtgcagtg atcgacgttg ttggttccga
     7321 agccctggcg gatcagcttc tggaacagat aggcctcttc gttcgatccc ttggccgagc
     7381 cgaagccggc gatggcgccg ccgccatgct cggccgcgat ggcagtcagg ccggaggcgg
     7441 cgcgcgccag cgcctcctcc cagctcgcct cgcggaacgt gtcccagggg ttggcgggat
     7501 cgatggtcgc ctcgccgcgc tttggcgcgt cgtcgcggcg gatcagcggc ttcgtcaggc
     7561 ggtgcgggtg atggatgtag tcgaaaccga agcggccctt gacgcagagg cggctgtcat
     7621 tggccggtcc gtcgcggccg tcgaccttga cgatgcggtt gtccttgacg cccacgcggg
     7681 tctggcagcc gacgccgcaa tacgggcaga gcgtatcgac ccagcgctcc gggacaacag
     7741 cgcgccggcg ggcggccggg tccatcagcg ccttctcgta cagcgcgccg gtcgggcagg
     7801 cctgcacgca ttcgccgcag gcgacgcagg tggagtcgcc catcgaatcg gcgagatcga
     7861 agatcgggcg cgtctcgtgg ccacgccggc ccatgccgat gacatcgttg acctgcacct
     7921 cgcggcaggc gcgctcgcac aggccgcaag cgatgcaggc gtccagattg acggcgatgg
     7981 cgggatgcga ggcgtcgtgg aggggcagnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8101 nnnnnnnncg ccggcatgtc ggcttcgagc agttcgaaga ccatgcggcg cgccttttcc
     8161 acgcgctcgc caccggtctt cacggccatg ccgtccgccg gcttgcggat gcaggaggcg
     8221 gcaagggtgc gttcgcccgc gatctcgacg acgcaggcgc ggcagttgcc gtccggccgg
     8281 taacccttct gctcgcaatg gcagagatgg gggatttcca cgccctcacg ccttgccacc
     8341 tgccagagcg tttcctccgg cccggcctcg acgggccggc catcaagggt gaagcggatt
     8401 gtcgcgctca tggctggctc ccggcgaaaa acttcaggat cgaccgcatc gggttcggcg
     8461 cggcctgtcc gagtccgcag atggacgcgt cgcgcatcac ctgctctatg tcggccatca
     8521 gggcctcgtc ccaggtcccg gcctgcatca gcgcgccgag cttttccgtg ccggcgcggc
     8581 agggtgtgca ctggccgcag ctttcatggg cgaagaaggc catgagattg cgggcgatgg
     8641 cgcggggatc gtcccggtcc gagaacacga cgatggcgtg actgccgacg aagcagccgt
     8701 gcttttccag cgtgccgaag tcgagcggtt catcggcgag ggcggcaggc agaatgccgc
     8761 cggaagcccc gcccggcagg tagccggcga ggcgatggcc ctccgccatg ccgccgcaga
     8821 actcggcgac aagttcattc agcgtgatgc cggccggggc gagcttgacg ccgggcttgc
     8881 ggacgcggcc ggagaccgaa taggagcgca cgcctttcgc gccgcgccgc ccctgccgtg
     8941 cgaaccactc cgcgcccttt tcggcgatgt cgcgcaccca gaacagtgtc tcgacattgt
     9001 ggttgagggt cgggcggccg aagaggccct tctcggcgat gtagggtggg cgatggcgcg
     9061 gcaggccgcg cttgccttcc agcgattcca gcatggcgct ttcctcgccg cagacatagg
     9121 cgccggcgcc gcgtctcagt tcaaccggcg tggtcagcag gccggcctgc tccagcgcgg
     9181 cgatctcgcg cagcaggatg gcgcgcacgc cggggtattc gtcgcgcaga tagatgtaga
     9241 tgcgctcggc cgaaacgaca tgggcggcga tgagcgcgcc ctccagcatg cggtgcggat
     9301 cggattcgag cagcacgcgg tccttgaagg tgccgggctc gccctcgtcc gcgttgatcg
     9361 tcatcagctt cgcgcccggc tgcgcccgga cgatggacca tttgcggccg gcgggaaatc
     9421 ctgcgccgcc gaggccgcgc aggccggcgt cggacagcag ggcgattagg tcttc
//
LOCUS       DAOMXN010000086         9456 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_3827_length_9456_cov_11.188491, whole genome shotgun sequence.
ACCESSION   DAOMXN010000086 DAOMXN010000000
VERSION     DAOMXN010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 9456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9456
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3827_length_9456_cov_11.188491"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(344..3298)
                     /gene="polA"
                     /locus_tag="PK812_07620"
     CDS             complement(344..3298)
                     /gene="polA"
                     /locus_tag="PK812_07620"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019443501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HRJ69462.1"
                     /translation="MKPGDHVFLVDGSSYIFRAYHALPPLTRKSDNLPVGAVSGFCNM
                     LWRLLRDTIAGEKPTHLAVIFDHSSQTFRNALYDQYKAHRPDPPEDLRPQFALVKAAT
                     LAFDIPAVEQEGFEADDLIATYARLAREAGATVTIVASDKDLMQLVGSGVTMYDTMKD
                     RRIDVAEVIEKFGVPPEKVVDVQALAGDSVDNVPGVPGIGLKTAAQLITEYGDVETLL
                     SRAGEIKQPKRREALLENAEKARISKALVRLEDKVPLAHGIESLGVHDPEPGRLIGFL
                     KAMEFTTITRRVADATGAKADEIAPDTRFVGAAAWNGRKPAETVPDTAEAAASAQATP
                     ATSDVAAKSHSADGRSGPEALVDFRKASVYKEKIETTAYETVTTLARLKDWAAMAREA
                     GAVAFDTETTSLDPMQAELVGFSLCVAPGRACYVPLAHKGEGTGDLFGGGALLPGQIP
                     VREALAALSEILVEPAILKIGQNLKYDVLVMLQHGIRIVSSDDTMLMSYVLDAGLHGH
                     GMDELSELHLGHKPIGFAEVAGTGKSAVTFDRVPIDRATAYAAEDADVTYRLWQVLKP
                     RLAAEGMTTVYETLERGMAGVLARMEQAGIAIDRTILSRLSGDFAQGMARLEAEVHSL
                     AGGPFNLASPKQLGDILFGKMGLPAPKKTATGAWSTGAQVLEDLAEQGHELPRRILDW
                     RQLAKLKSTYTDALPTYLNARTNRVHTSFALAATTTGRLSSSEPNIQNIPVRTEEGRK
                     IRTAFVATPGHRLISADYSQIELRLLAHIAEIPQLRQAFADGVDIHAMTASEMFGVPV
                     KDMPSDVRRRAKAINFGIIYGISAFGLANQLGIPREEAGAYIRKYFERFPGIRDYMED
                     TKRFARANGYVATVFGRKCHYPQINSKNPSERAFMERAAINAPIQGSAADIIRRAMLR
                     MEAALDDAKLSTRMLLQVHDELVFEAPEAEVDRAIPLIRQVMVDAPHPALTLKVPLQV
                     DARAAQNWDEAH"
     gene            3386..4051
                     /locus_tag="PK812_07625"
     CDS             3386..4051
                     /locus_tag="PK812_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRJ69463.1"
                     /translation="MAVMQPQVLDFQYRDPEDVLERARQFRNLLRPRRTVRDFSDRPV
                     PREVIEQLVMTAGGAPSGANQQPWTFVCISDPDTKRRIREAAEEEEKAFYAGKAGEEW
                     LEALEHLGTDWEKPFLERAPWLIAIFAHRHGVGPDGARIKHYYVPESVGIASGFLIAA
                     CHNAGLASLTHTPAPMGFLNEICGRPDNEKPVILLVVGYPAETCTVPTIRKKPLEEIA
                     VFI"
     gene            complement(4108..5793)
                     /gene="rpsA"
                     /locus_tag="PK812_07630"
     CDS             complement(4108..5793)
                     /gene="rpsA"
                     /locus_tag="PK812_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HRJ69464.1"
                     /translation="MSVSASPAREDFAALLEESFGRNDMAEGSVIKGKVVAIEKDDAV
                     IDIGLKTEGRVKLKEFSVPGREQALKVGDEVEVYLERVENALGEAVISREKARREESW
                     VKLEQAFEKQEKVNGIIFNQVKGGYTVDLEGAVAFLPRSQVDIRPIRDVTPLMGVAQP
                     FQILKMDRRRGNIVVSRRTVLEETRAEARSELVANLEEGQTVDGVVKNITDYGAFVDL
                     GGIDGLLHVTDIAWRRVNHPTEVLTVGQTVKVKIIKINQETHRISLGMKQLMGDPWAG
                     IEAKYPLQAKFKGRVTNITDYGAFVELEPGIEGLIHVSEMSWTKKNVHPGKILSTSQE
                     VEVQILEVDSSKRRISLGLKQTLANPWEAFVDKHPVGSIVEGEVKNKTEFGLFVGLDG
                     DVDGMVHLSDLDWNRPGEQVLEEFRKGDVVKAIVLDVDVEKERISLGVKQVAGDPFAD
                     AGEIKKGQVVTCEITEVKDSGLEVKISGTDLSTFIKRSELARERSDQRPERFAAGEKV
                     DARVILFDRKARKVQVSIKALEVAEEKEAMAQYGSADSGATLGDILGAALKRK"
     gene            complement(6024..6632)
                     /locus_tag="PK812_07635"
     CDS             complement(6024..6632)
                     /locus_tag="PK812_07635"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0036431 - dCMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006220 - pyrimidine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="d(CMP) kinase"
                     /protein_id="HRJ69465.1"
                     /translation="MIIAVDGPAASGKGTLARRLAAHFQLPHLDTGLLYRAVGRKMLD
                     AGRDFSDAEAAIGLARNFAPAWLVDGRLRGNEASVAASRVASIPEVRSALREFQQQFA
                     GQPGGAVLDGRDIGTVIAPQADVKLWVTAAPEVRARRRYLELKPKRPELQEATILSEL
                     IARDGRDAPNMIMADDAHLLDTSAMDIEQAFQAAVAIVEQAK"
     gene            complement(6643..6855)
                     /locus_tag="PK812_07640"
     CDS             complement(6643..6855)
                     /locus_tag="PK812_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69466.1"
                     /translation="MSAYVLIVVISQILGGSGTAANATQYAVGEYPSMEQCRKAARTS
                     EVVHSVNANAGPKPRIDFICVQRSKL"
     gene            complement(6928..8241)
                     /gene="aroA"
                     /locus_tag="PK812_07645"
     CDS             complement(6928..8241)
                     /gene="aroA"
                     /locus_tag="PK812_07645"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HRJ69467.1"
                     /translation="MTATVSGPLKGRVRVPGDKSISHRSLIFGLLALGESRVSGLLEG
                     EDVIRTAAAARALGATVDRLSPGEWSVRGVGVGGLLQPTGTLDFGNAGTGSRLMMGVV
                     GGHPITARFDGDASLRKRPMGRILEPLRLFGIRAVETAPGDRLPLTVQGARNPVPITY
                     ETPVPSAQVKSAILFAALNAPGKTVVIEREATRDHTEKMLAYFGAEVLVEKHGPHGRR
                     ITLTGQPELRPCHIVVPADPSSAAFPIVAALIVPGSDVTVEAVMMNPLRIGLLTTLRE
                     MGADIAETNRRIEGGEEVADLRVRHSALQGVSVPAERAPSMIDEYPILAVAAAYAAGE
                     TRMNGLAELRVKESDRLAAVADGLTANGVRNRIEGDDLVVSGAAGQAPGGGLVATHMD
                     HRIAMSFLVMGLAAQKPVRVDDTAFIMTSFPQFVPMMRGLGGALS"
     gene            8443..8817
                     /locus_tag="PK812_07650"
     CDS             8443..8817
                     /locus_tag="PK812_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02300 family protein"
                     /protein_id="HRJ69468.1"
                     /translation="MAKPELGMKRACLSCGAKFYDLNRDPILCPKCGAQFQVQVMRRP
                     EAAKSEDEEEAVVDPAAADLVPLEEAEAGASDKELPGDDIDIDEDIGGDADDTFLEQE
                     EEEDESNVSDLIGGDLEEDEEN"
     gene            8959..9034
                     /locus_tag="PK812_07655"
     tRNA            8959..9034
                     /locus_tag="PK812_07655"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8992..8994,aa:Ala,seq:ggc)
BASE COUNT         1632 a         3046 c         3089 g         1689 t
ORIGIN      
        1 gccgtcgtcg cggcctgcgg cggtgtgcgg acctgctggg ccaacgcggc gatctcggcc
       61 tgaatgcgct gcgcggatgt catgccgtcc attatctgcc ccgtcggcgg tcaaatccgg
      121 ggcagttatc gccaagtcag gttaatttga cgttcagatg cgcgcttcgt gggggccggg
      181 cggcgcgcct agagcagttt ccgaccggat tggatcaatt ccgtttcgcg acaaatgctc
      241 ggtaaagccc ctcgaccgca taccggatac gcacttcggg cctttcccgg tccttgtcgc
      301 gaaattgccc ggtcggaatg gcatgatctg atcggatcgt gctctagtgc gcctcgtccc
      361 agttttgtgc ggcgcgggca tccacctgca acggcacctt gagcgtcagc gcggggtggg
      421 gcgcgtccac catcacctgg cggatgagcg ggatggcgcg gtccacctcg gcctcgggcg
      481 cctcgaacac aagttcgtcg tgcacctgca gcagcatgcg cgtcgaaagc ttcgcgtcgt
      541 cgagcgcggc ctccatgcgc agcatggcgc ggcggataat gtcggccgcc gagccctgga
      601 tgggcgcgtt gatcgcggcg cgttccatga aggcgcgttc cgaaggattc ttcgagttga
      661 tctgcggata atggcacttg cgcccgaaga ccgtcgcgac atagccgttg gcgcgggcaa
      721 agcgcttggt gtcctccatg tagtcgcgga tgccggggaa gcgttcgaaa tacttgcgga
      781 tgtaggcgcc ggcctcctcg cgcggaatgc caagctggtt ggcgaggccg aaggcggaga
      841 tgccgtagat gatgccgaag ttgatggcct tggcccgccg ccgcacatcg gacggcatgt
      901 ccttgaccgg cacgccgaac atctcgctgg ccgtcatggc atggatgtcg acgccgtcgg
      961 cgaaggcctg ccggagctgc ggaatctcgg cgatgtgggc gagcagccgc agctcgatct
     1021 ggctgtagtc ggccgagatc agcctgtgtc cgggcgtcgc cacgaaggcg gtgcggattt
     1081 tccgaccctc ctcggtgcgc accggaatgt tctggatgtt cggctccgag gaggagagcc
     1141 gtcccgtcgt cgtcgcggcc agggcgaagg acgtgtgcac gcggttggtg cgcgcgttga
     1201 ggtacgtcgg caaggcgtcg gtataggtcg acttgagctt cgcgagctgg cgccagtcga
     1261 ggatgcggcg cggcagttcg tggccctgct ccgccaaatc ctccagaacc tgcgcgccgg
     1321 tggaccaggc accggtcgcg gtcttcttgg gcgcgggcag gcccatcttg ccgaacagga
     1381 tgtcgccgag ctgtttgggc gacgcgaggt tgaatggtcc ccccgccagg ctgtgaacct
     1441 ccgcctccag ccgcgccatg ccctgggcga aatcgccgga caggcgcgag aggatggtgc
     1501 ggtcgatggc gatgccggcc tgctccatgc gtgccagcac gccggccatg ccgcgttcca
     1561 gcgtctcgta gaccgtggtc atgccctcgg cggcaaggcg gggcttgagc acctgccaga
     1621 gccggtaggt gacgtcggcg tcctccgccg cataggcggt ggcgcggtca atcggcacgc
     1681 gatcgaaggt cacggcgctc ttgccggtgc cggccacctc cgcaaagccg atcggcttgt
     1741 ggccgagatg caactcactc aactcgtcca tgccatggcc gtgcagccct gcgtcgagca
     1801 catagctcat cagcatggta tcatcgctcg aaacgatgcg gatgccgtgc tggagcatca
     1861 ccagaacatc gtatttcagg ttctgtccga tcttgaggat cgccggttct acgaggattt
     1921 cggacagcgc cgccagcgct tcgcgcaccg ggatctgtcc gggcagcagc gcgccgccgc
     1981 cgaacaggtc acccgtgcct tcgcccttgt gagcaagtgg cacatagcag gcccggcccg
     2041 gtgccacgca gagcgagaag ccgaccagtt cggcctgcat cgggtcaagc gaggtggttt
     2101 cggtgtcgaa agcaaccgct cccgcctccc gcgccatcgc cgcccaatcc ttcagccggg
     2161 cgagcgtggt caccgtctca taggccgtcg tttcgatttt ctctttgtaa acagaagctt
     2221 tccggaaatc gaccagagct tctggaccgg aacgaccgtc tgcggagtgg ctcttggctg
     2281 cgacgtcact ggtggcgggt gtggcctgcg cggacgcggc tgcttcagcc gtgtcgggta
     2341 cggtctcggc cggcttccgc ccgttccagg ccgccgcacc gacaaagcgc gtgtcgggag
     2401 cgatctcgtc cgccttcgcg ccggtcgcat ccgcgacgcg acgggtgatg gtggtgaatt
     2461 ccatcgcctt gaggaagccg atcagccggc cgggctcagg atcatggacg ccgaggctct
     2521 cgatgccatg ggccagcgga accttatctt caagtcgaac caaggccttg gaaatacggg
     2581 ctttttcagc gttttccagc agcgcttcgc ggcgcttcgg ctgcttgatc tcaccggcgc
     2641 gggacagcag ggtctcgaca tcgccgtatt cggtgatgag ctgcgccgcg gtcttgaggc
     2701 cgataccggg cacgcctggc acgttgtcca ccgaatcccc ggcaagcgcc tgaacatcca
     2761 cgactttctc gggcggcacg ccgaatttct cgatgacttc cgccacatcg atgcgccggt
     2821 ccttcatcgt gtcgtacatg gtcacgccgc tgccgacgag ctgcatcaga tccttgtcgg
     2881 aagcgacgat ggtgacggtc gcgccggctt cgcgcgccag ccgggcatag gtggcgatga
     2941 ggtcgtcggc ctcgaaccct tcctgctcga ccgccggaat gtcgaaggcg agcgtcgccg
     3001 ccttcaccag cgcgaactgc ggcctgaggt cttccggcgg atcgggccgg tgcgccttgt
     3061 attggtcgta gagagcgttg cggaaggtct gcgaggaatg atcaaagatt acagctagat
     3121 gcgtcggctt ctcgcccgcg atcgtatcgc gcagcagtcg ccagagcatg ttgcagaacc
     3181 ccgacaccgc gccgaccggc aggttgtcgc tcttgcgcgt cagcggcggc agggcgtgat
     3241 aggcgcggaa gatgtaggac gaaccgtcga ccagaaagac atgatcgccg ggtttcaggg
     3301 gacggtgcga aaaggacatg gcgaaagcat agtgcgcccg cgtccgcccg gctatcgtct
     3361 tgataacgga tcaggagaaa cggtcatggc cgtgatgcaa ccgcaggtgc tggacttcca
     3421 gtatcgagac ccagaggacg tactggaacg ggcgcgccag ttccgaaacc tgctgcgacc
     3481 acggcggacc gtgcgcgact tttccgaccg cccggtgccg cgcgaagtca tcgaacagct
     3541 ggtgatgact gcgggcggcg ccccgtccgg cgccaatcaa cagccctgga cgttcgtctg
     3601 catttcagac ccggacacga agcgtcggat ccgcgaggcg gccgaggagg aggaaaaggc
     3661 gttctacgcc ggcaaggccg gcgaggaatg gctggaagcg ctggagcatc tcggcacgga
     3721 ttgggaaaag cctttcctcg aacgcgcgcc ctggctgatc gcgatcttcg cccacagaca
     3781 tggcgtcggc ccggatggcg cccggatcaa gcactactac gtgccggaat cggtcggaat
     3841 cgcgtcgggc ttcctgatcg cggcctgcca taacgcgggc cttgcaagcc tcacccacac
     3901 ccccgcgcca atggggtttc tcaacgaaat ctgtggtcgg ccggacaatg aaaagcccgt
     3961 gatcctgctg gtggtcggct atcccgccga gacctgcacc gtgccgacga tccggaagaa
     4021 gccgctcgaa gagatcgccg tctttatctg aaatgaaaac cccggcgttt ccgccggggc
     4081 tctgaaggtt gtcctggtct ttggcgctta cttgcgcttg agcgccgcgc cgaggatgtc
     4141 gccgagggtg gcaccggaat cggccgagcc gtactgggcc atggcttcct tctcttccgc
     4201 cacttcgagc gccttgatcg acacctggac cttgcgggcc ttgcggtcga acaggatgac
     4261 gcgcgcgtcg accttctcgc ccgccgcgaa ccgctccggc cgctggtccg agcgctcgcg
     4321 cgccagttcc gagcgcttga tgaaggtgga gaggtccgtg ccggagatct tcacctcaag
     4381 gccggaatcc ttcacctcgg tgatctcgca ggtgacgacc tggcccttct tgatctcgcc
     4441 ggcgtcggcg aagggatcgc ccgcgacctg cttgacgccc agcgaaatgc gctccttctc
     4501 gacgtcgacg tcgagaacga tggccttcac cacgtcgccc ttgcggaact cctcgagcac
     4561 ctgctcgccc ggacggttcc agtcgaggtc ggagagatgg accatgccgt ccacatcgcc
     4621 gtcgagaccg acgaacaggc cgaattcggt cttgttcttg acctcgcctt cgacgatcga
     4681 acccaccggg tgcttgtcga caaaggcttc ccacgggttg gccagcgtct gcttgaggcc
     4741 gagcgagatg cgacgcttcg aggaatccac ctcgagaatc tgcacttcga cttcctgcga
     4801 ggtcgaaagg atcttgccgg ggtggacgtt cttcttcgtc caggacattt ccgagacgtg
     4861 gatgaggcct tcgatgcccg gctccagttc gacgaacgcg ccgtagtccg tgatgttggt
     4921 gacgcgaccc ttgaacttgg cctgcagcgg gtacttcgcc tcgatgccgg cccagggatc
     4981 gcccatcagc tgcttcatgc cgaggctgat gcggtgcgtc tcctggttga tcttgatgat
     5041 cttgaccttc acggtctggc cgaccgtcag cacctcggtc gggtggttga cgcggcgcca
     5101 ggcgatgtcg gtcacgtgca gcaggccgtc gatgccaccg aggtcgacga acgcgccgta
     5161 gtcggtgatg ttcttgacga cgccgtcgac ggtctggcct tcctcgaggt tggcgacgag
     5221 ttccgaacgg gcctcggcgc gggtctcttc cagcacggtg cggcgggaga cgacaatgtt
     5281 gccgcggcgc cggtccatct tcaggatctg gaacggctgg gcgacgccca tcagcggggt
     5341 cacgtcgcgg atcggacgga tgtccacctg cgagcgcggc aggaaggcga ccgccccttc
     5401 cagatcgacg gtgtagccgc ccttgacctg gttgaagatg atgccgttga ccttctcctg
     5461 cttctcgaag gcctgctcga gcttcaccca gctctcttcg cggcgcgcct tctcgcggga
     5521 aatgacggct tcgccgaggg cgttttcgac acgctcgaga taaacttcga cctcgtcgcc
     5581 aaccttgagc gcctgctcgc ggccgggaac ggaaaattcc ttcagtttga cacggccttc
     5641 ggtcttgagg ccgatatcga tcaccgcgtc atccttctcg atggcgacga ccttgccctt
     5701 gatgaccgaa ccttcggcca tatcgttacg gccgaacgac tcttcgagca gcgcggcgaa
     5761 atcttcacgg gcgggggagg cggaaacgga catgtatgct ccggatgagg cctggccctg
     5821 acctcgaacg ttgcgcggct ctggtttgtg gtcgcgtccg gtcgccggcc gcttccaggc
     5881 ctggaagcgt gaacggctcc catgcgggag gccgtcctgc cggcgcgttg aaccggcgat
     5941 cggtcgcgtg aagtctgcag tctcttccgg gcggagaccg gaaatgcccg gtcaaagcaa
     6001 aaagggcacg gttgcccgca ccctcatttg gcctgctcga caatcgcgac agccgcctgg
     6061 aacgcctgct ctatatccat tgcgctggtg tcgagcaagt gggcgtcgtc ggccatgatc
     6121 atgttgggcg cgtcgcgccc gtcccgggcg atgagttcgg acaagatcgt cgcctcctgc
     6181 agctccggcc gcttgggctt gagctcaagg tagcgccggc gcgcgcgcac ctcgggtgcc
     6241 gcagtcaccc acagcttcac gtccgcctga ggtgcgatca cggtgccaat gtcgcggccg
     6301 tcgagcacgg cgccgccggg ctggccggcg aactgctgct ggaactcgcg cagggccgac
     6361 cggacttccg gaatcgaggc caccctgctg gcggccacgc tggcctcgtt gccgcgcaga
     6421 cggccatcca ccagccaggc aggcgcgaaa ttccgcgcca atcctatcgc ggcctcggca
     6481 tccgaaaaat ccctgccggc atcgagcatc tttcggccaa ccgcgcgata gagcagaccg
     6541 gtatcgagat gcggcagttg aaagtgcgcg gcgaggcgac gcgcaagcgt ccctttgccg
     6601 gaagcggcgg ggccatcgac ggcgataatc atgcggcgct tatcagagct tcgagcgctg
     6661 gacgcaaatg aaatcgatgc gcggcttcgg tccggcgttc gcattgacgg aatgcaccac
     6721 ttcggaggtg cgcgcggcct tccggcactg ttccatcgac ggatattcgc cgactgcata
     6781 ctgcgtggcg ttggcggcgg tgccagaccc gccgaggatc tgagagatca cgacgatcag
     6841 gacataggcg gacatcgtaa gggctccggg ttgtccgcga accataacgc ctgacgcccg
     6901 acccgtgcgc cgaaactgaa gaaagggtta agacagcgcg ccgcccagcc cccgcatcat
     6961 cggcacgaat tgcggaaaac tcgtcatgat gaaggccgta tcatccacgc gcaccggctt
     7021 ttgcgccgcc agccccatga ccaggaagct catggcgatg cggtgatcca tgtgcgtcgc
     7081 cacgagcccg cctccgggag cctgcccggc ggcgccggag acgacaaggt cgtctccctc
     7141 gatgcgattg cgcacaccgt tggcggtcag cccatcggcg acggccgcga gtcgatcgct
     7201 ttccttcacg cgcaactcgg caagtccgtt catgcgcgtc tcgccagcgg cataggcggc
     7261 cgcgacggca aggatgggat actcgtcgat catcgacggc gcccgctcgg ccgggaccga
     7321 cacgccttgc agcgcggaat ggcgcacccg caggtcggca acctcctcac cgccttcgat
     7381 acgccggttc gtctccgcga tgtccgcgcc catctcgcgc agcgtggtga gcagcccgat
     7441 gcgcagcggg ttcatcatca cggcctcgac cgtcacgtcc gatccgggaa cgatcagcgc
     7501 cgcgacgatc ggaaaggctg ccgacgacgg atcggccggc acgacgatgt gacagggcct
     7561 gagttcaggc tggccagtga gcgttatccg gcgaccgtgc ggtccgtgct tctcgaccag
     7621 gacttcggcg ccgaaatagg ccagcatctt ttccgtatgg tcgcgcgtcg cctcgcgctc
     7681 gatcacgacg gtcttgccgg gagcgttgag ggccgcgaac aagatcgccg atttcacctg
     7741 cgccgagggt acgggtgtct cgtatgtgat cggaacggga tttctcgcgc cctgcaccgt
     7801 caagggcagg cgatcgcccg gcgccgtctc aacagcacga atcccgaaca gcctgagcgg
     7861 ttcgagaatg cgccccatcg gccgcttgcg cagcgaggcg tctccatcga accgcgccgt
     7921 gatgggatgg ccgccgacaa cgcccatcat gagtcgcgag ccggtgccgg cattgccgaa
     7981 gtccagagtc ccggtcggct gcagcaatcc cccgacgccc acgccgcgca cgctccactc
     8041 ccccggggag agccgatcga ccgtggcgcc gagcgcccgg gcggcggcgg cggtgcggat
     8101 gacatcctcg ccttccagca gacccgatac ccgcgattcg ccgagggcaa gcagaccgaa
     8161 aatgaggctc cggtgcgaga tggatttatc gccggggacg cggacccgtc ccttcagggg
     8221 gcccgagacg gtggcggtca tcggcgcggg cgcggcggcg tgagatgtcg acaaggcaaa
     8281 ctccaggcga aacggtgcgg cacagtcgcg ggaacagcga cccgctagca cgttacgacc
     8341 gcccggtcag cgggtcatgc gcgaattgtc gccaagacca tttgacagga gccgctgcgc
     8401 cgactaacgg gcggaaaacc caaacgacag gatcatcaag acgtggccaa gccagaactc
     8461 ggcatgaagc gcgcgtgcct cagctgcggc gcgaaattct acgacctcaa tcgtgatccg
     8521 atcctgtgcc cgaaatgcgg ggcccagttc caggttcagg tgatgcgccg gccggaagcc
     8581 gccaagtcgg aagatgagga agaggcggtt gtcgatccgg cagcggccga tctcgttccc
     8641 ctcgaggaag cggaagccgg cgcttccgac aaggagcttc cgggcgacga catcgacatc
     8701 gacgaagaca tcggtggcga tgccgacgac accttcctcg aacaggagga agaagaggac
     8761 gagagcaatg tctccgacct catcggaggc gacctcgagg aagacgagga gaactgatcg
     8821 gctgcccgat cacatcgacc aggcgggaat ggcaggattg cctcttgcgt ccgcccgccc
     8881 gatgtgacac atgcaccgcg cctgacggcc aagcgccgtc gggcttcgcg ccggctccca
     8941 tccggcgcta tggacttagg ggctatagct cagctgggag agcgcttgaa tggcattcaa
     9001 gaggtcagcg gttcgatccc gcttagctcc accatccttc tcctcaggaa aacggcatca
     9061 cttgcggcaa ggtgattttc aggaccgccc cggaaatcgc gtgccggtcg ccggccccgt
     9121 tccgcgtcga tactccgcgc ggctcatgac ctgaaagccg cctgctcact tgttgatggg
     9181 acagtcttgc gggtgatcgc ggactccgcc gcgggatcgc tctgtcgaac tgattcacgc
     9241 ggctcgcgaa acgccctggc ggcgcgtctg ccggctggcg cacggccgac ggctccgttg
     9301 gctcctggcg aaggcgtgcc aaagcctgcg aaagactttt cgcacgaaat aactgaattt
     9361 ccggaatttt cgaaggctgg cgggacatct cgaagggtga gatggtgccc aggagaggac
     9421 tcgaacctcc acggctttca ccactggtac ctgaaa
//
LOCUS       DAOMXN010000087         9149 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_3998_length_9149_cov_10.078183, whole genome shotgun sequence.
ACCESSION   DAOMXN010000087 DAOMXN010000000
VERSION     DAOMXN010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 9149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9149
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3998_length_9149_cov_10.078183"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..416
                     /locus_tag="PK812_07660"
     CDS             27..416
                     /locus_tag="PK812_07660"
                     /inference="COORDINATES: protein motif:HMM:NF022227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliX"
                     /protein_id="HRJ69469.1"
                     /translation="MRTEQPADTPRSVGARSLSGLILGPEPVDPDSRRRRAMRQGHRI
                     LDVLDDLKVVALGACDGAKALAQLAAELGALEVPGDERLAALLDQVRIRGEVELAKAE
                     AAAALRRQTIPHRHESPGLVPAPLPGS"
     gene            730..1161
                     /gene="dksA"
                     /locus_tag="PK812_07665"
     CDS             730..1161
                     /gene="dksA"
                     /locus_tag="PK812_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HRJ69470.1"
                     /translation="MAKKAKEIRLDPGYRPTDAEPFMNDRQREYFRRKLINWKEEILR
                     DAEDTVTAMQSDTEAHPDLVDRASSEGDRAIELRARDRQRKLISKIDAALARIEDGSY
                     GFCEETGEPIALQRLDARPIATLSLEAQERHERREKIYRDD"
     gene            1174..1644
                     /locus_tag="PK812_07670"
     CDS             1174..1644
                     /locus_tag="PK812_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007067829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HRJ69471.1"
                     /translation="MKPATAESARKVQAILGPRFTVLEFEASTRTAAEAAAAIGCTQE
                     QIAKSLLFRSDSGRPVLVIASGAVRVSERKVAALLGEEIGRADAAFVRAMTGFAIGGV
                     PPVGHAVTPWVAIDDTLARLDTIWAAAGTPNAVFALTPADLAALTGGSFADVAA"
     gene            complement(1656..2369)
                     /gene="flgH"
                     /locus_tag="PK812_07675"
     CDS             complement(1656..2369)
                     /gene="flgH"
                     /locus_tag="PK812_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HRJ69472.1"
                     /translation="MAAMPLTACGSIDRIANIGQPPPLSAIENPVAQPGYKPVQMPMP
                     MPQPSVHASNSLWRTGSRAFFKDQRASQIGDLLTVKVRITDSAKIDNQTQRSRQNGEN
                     LGVEGLFGYETKFSKVFDENVKPEALVKGSSSSNSQGAGSVKRQEQLATDIAAVVTQV
                     LPNGNLVIEGKQEVRVNFEVRELVVAGVVRPEDIEAGNAVDSTKIAQARISYGGRGQI
                     TDVQQPRYGQQVMDILLPF"
     gene            complement(2425..3354)
                     /gene="flgA"
                     /locus_tag="PK812_07680"
     CDS             complement(2425..3354)
                     /gene="flgA"
                     /locus_tag="PK812_07680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HRJ69473.1"
                     /translation="MRRSIIAMVCALACAGAFAQTPALKPAAVIQGGVVRLGDIVEHA
                     GALAATPLFRAPDLGKSGMISAERIIEAGREAGLETIDARGLTAVVVRRPSRLVSAQE
                     IEQSVLEAIRRSDTSLTEATLRFDTPPPQLARDAINTAEIRTEVVTLDRRLERFEAHL
                     VLPDQPGEPVRLSGSYDTRLSLPVPTRDIPRGETIQASDLRLEQRRRRDIPAGALTEP
                     ARLTGFVARKALRVGQPLRDGDVVRPDMVERNQLVTVLYESAGMTLSLRGKALAAGAE
                     GAIVAVQNLQSKRTIETIVIGPGRVSARHHPAE"
     gene            complement(3367..4158)
                     /gene="flgG"
                     /locus_tag="PK812_07685"
     CDS             complement(3367..4158)
                     /gene="flgG"
                     /locus_tag="PK812_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003606772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HRJ69474.1"
                     /translation="MRALQTAATGMMAQELNVEVISNNVANMRTTGFKRMRVQFQDLL
                     YETHKRAGSPSSDQGTSVPAGIYVGSGVKSVSTPRIMGQGNLSPTEKSYDLAIRGEGY
                     FRVQLPDGRTGYTRDGSFELDADGRMVTAEGYLLDPAITVPNTAKGISINAQGVVSAS
                     LPGQVQPQQLGQIQLARFVNKTGLESIGDNLFVETQGSGTAITGNPNADGFGTLQQGY
                     LEESNVNAVTEISALIAAQRAYELNAKVVQAADQMLSATSQAFRG"
     gene            complement(4166..4906)
                     /gene="flgF"
                     /locus_tag="PK812_07690"
     CDS             complement(4166..4906)
                     /gene="flgF"
                     /locus_tag="PK812_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020093628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HRJ69475.1"
                     /translation="MENIAPISLSRHIALQRAMDVIANNVANSATNGFKRRSGMFNEH
                     VAPRARADSLRPADRAISFVIDRGSTLDLSQGAADRTGNPLDVTIKGDAFFVVRTSSG
                     ERYTRNGALSLDARGELVTSDGHAVLAEQGPLAFTAADRDIAIAADGTVSTAQGVRGR
                     LKLAAFTRPSVLENIGGNLFSAREAPRPASAEIRLESGVLEKSNVSPVVEISRMIEVS
                     RAYTSLAQSMQRGDDLRRSAITKLAEAA"
     gene            5155..5634
                     /locus_tag="PK812_07695"
     CDS             5155..5634
                     /locus_tag="PK812_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HRJ69476.1"
                     /translation="MAKSKKGDDKQADGESPPKGRKLLLIGAAVVVLAAGGGGFWWFK
                     LKPSGAKAEMKKVAVFLDLPDMTVNLAQGAGNERQSFLKLKVSLEVADQKTLAEIQPV
                     MPRLLDNFQVFLRELKVSDLEGSAGLYRLKEELVRRTNIAVHPAKVEAVLFKDILVQ"
     gene            5643..6641
                     /locus_tag="PK812_07700"
     CDS             5643..6641
                     /locus_tag="PK812_07700"
                     /inference="COORDINATES: protein motif:HMM:NF014235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HRJ69477.1"
                     /translation="MQDFSGNTVRAAEVSAPSTVPVPGSQPTARPEQAGRAGVALTDI
                     CGRGERAPVVLPMLDLALDRVPDILGRRLSGAWNGRVTAAASGRRVARFDALEAELNG
                     LRLVGLLRHAENPDFGLVVFPAQSVWRLIDCMLGGSGGDLVDRQRFSHLERCLMRAPV
                     DQLLAGLGEAFEPVAPIGFDFVGWAEGLDAARPGRIGDPVLMQELTITLPRGDARFDL
                     LLPLSLFERHVEALAAPHPGERLGQDSLWKDSLLAEVRRATVEIEAVLYEGTAPVGRM
                     RALQPGDSYAFGLPPQTLLRLRAGGVDLALGQLGRSGSLAAVRFETPVEATTEARS"
     gene            6638..6988
                     /locus_tag="PK812_07705"
     CDS             6638..6988
                     /locus_tag="PK812_07705"
                     /inference="COORDINATES: protein motif:HMM:NF039465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6468 domain-containing protein"
                     /protein_id="HRJ69478.1"
                     /translation="MMAGLSVGLIIEALVALLLAVSIGYCVVLDRRLQRIRLNEDEMR
                     RTILELGAATERAERAIETLRDAVHDCDRTLADRLRQAERQSGDLVQQIRAGSEVLSR
                     ISRIVAVGKGGVAA"
     gene            6985..7791
                     /locus_tag="PK812_07710"
     CDS             6985..7791
                     /locus_tag="PK812_07710"
                     /inference="COORDINATES: protein motif:HMM:NF015413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlbB"
                     /protein_id="HRJ69479.1"
                     /translation="MTARRPRPRLLPVVIAAAASLLALKTLGLVTQGGYVLGPSAERE
                     RFARALTEPRRNFDPDPEPTGATPAKKDTKEPAKDAAAKDAPAPAKPADPSSQPLPPP
                     VPAAERQVLEKLSERRRQLDDRAKELDLREQLLKSAEQKIEERLGELKGGEKPAEDGG
                     APKDKSDAPFKSLVTIYEAMKPRDAAKVFERMEPRALAPLVSQMNPRKFSEILAQMTP
                     ESAEKLTQALMRRSDPAHGGARSQPAHSSETPKKPELEELPRLERQPKGT"
     gene            complement(7804..8010)
                     /locus_tag="PK812_07715"
     CDS             complement(7804..8010)
                     /locus_tag="PK812_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HRJ69480.1"
                     /translation="MRRQLVRFLPDEGGATAIEYGLIAALSALAVIPALDSMGGKLAS
                     TLNSIGQGLGKAPEAAPGMVPPGK"
     gene            8232..9131
                     /locus_tag="PK812_07720"
     CDS             8232..9131
                     /locus_tag="PK812_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69481.1"
                     /translation="MMRLLEIRSRWLARMQGMAACAAFVLVALTTSIRAQETTASVSA
                     RDTGGFGRIEFLFSLPTGFTARLSGSVLVVEFDRPVASNPEKLPLALPGYLAAARLDP
                     ERRSMRFALTRPARIDVKEAAERVFLDLLPTTWTGPPPPMPADVVRELARRAREAQAA
                     IRAAETRTAAQIRHAVSVRLAMREEGPRIVFESPEAGDARLDHNGSDLRIRFPPGAAL
                     NEAALRALLSELATDIRVEDGREGPVAFLRLRQNRQSRTLAEEGAFFVDLVADAPAAG
                     PVPRVEATVQEAKPVPAPPPRKR"
BASE COUNT         1499 a         2890 c         3146 g         1614 t
ORIGIN      
        1 ccgcagttca gccggcagag ttgctaatga gaacggagca gccggcggat acgccgaggt
       61 cggtcggcgc ccggagtctc agcggcttga tcctcgggcc ggagcccgtc gatccggatt
      121 cgcgtcggcg ccgcgccatg cggcaaggcc accgcattct ggatgttctc gacgatctca
      181 aggtggtcgc gctcggcgca tgcgacgggg caaaggcgct ggcgcagctt gcagccgaac
      241 ttggcgcact cgaagttccg ggcgacgaac ggttggcggc cctgctcgat caggtccgca
      301 tccggggcga ggtcgaactg gcgaaagccg aagccgcggc ggccctgcgc cgccagacga
      361 tcccgcaccg tcacgaatcg cctgggcttg tccccgcacc cttgccgggt tcgtgaaggg
      421 ctgctcgtgg cgtcttcgcc agggtgagac gcaacgctcc aggatgatcg cggaaaggat
      481 ctcccgcttt gccgggtgcg cgatctccct cccgatgggc aggaaccgca tgtcttttcc
      541 ggctttttcg gcgcgaatga catcctccag cggggggcgg ccggtcgcga agccccggaa
      601 cacgctcatg cgcgggcatt ttcgcaggga tttgaacgcg ctttgcaatt gttctgtcac
      661 agcgccggcg ctataggggc gtcctacgac ttaaggcgag gaaggccgct aatggcgacg
      721 aatcaagcaa tggccaagaa ggccaaggaa atccggctcg atccgggcta ccgcccgacc
      781 gacgccgagc ctttcatgaa cgaccggcag cgcgagtatt tccgacgcaa gctgatcaac
      841 tggaaggaag aaatcctgcg cgatgcggag gatacggtga ccgcgatgca aagcgacaca
      901 gaggcacacc ctgacctcgt cgaccgcgcc tcgtcggaag gcgaccgcgc catcgagttg
      961 cgtgcccgcg atcgccagcg caagctgatc tccaagatcg acgcggccct ggcgcgcatt
     1021 gaggacggtt cctacggttt ctgcgaggag accggcgaac cgatcgccct gcagcggctg
     1081 gatgcccgtc cgatcgccac gctgtcactt gaggcgcagg agcgccacga gcgccgcgag
     1141 aagatttatc gcgacgactg agccgagatt gccgtgaaac cggcgaccgc agagtccgcg
     1201 cgcaaggtgc aggccattct cggcccgcga ttcacggtgc tcgaattcga agccagcacc
     1261 cggacggcgg cggaggccgc cgccgctatc ggctgcacgc aagagcagat cgccaagtct
     1321 ttgctgtttc gctccgatag cggaaggccc gtgctggtca tcgcctccgg tgccgtgcgc
     1381 gtttcggagc gcaaggtcgc cgcgctgctg ggcgaggaga ttggccgggc cgatgcggcg
     1441 ttcgtgcggg ccatgacagg ctttgccatt ggcggggtgc cgccggtggg gcacgccgtc
     1501 acgccctggg tggccattga tgacacgctt gcccggctcg atacgatctg ggcggcggcg
     1561 ggcacgccga acgcggtgtt cgcgctgacg ccggccgatc tcgcggcgct gaccggggga
     1621 agtttcgcag acgtcgcggc ctgaggcgcg gcggatcaga acggcagcag gatgtccatt
     1681 acctgctggc cgtagcgcgg ttgctgaaca tcggtgatct gtccgcgacc gccgtaggaa
     1741 atgcgcgcct gcgcgatctt ggtggagtcg acggcgttgc cggcctcaat gtcttccggt
     1801 cgcaccacgc cggcgacgac cagttcgcgc acttcgaaat tgacgcgcac ctcctgtttg
     1861 ccctcgatga cgaggttgcc gttcggcagg acctgcgtca ccacggccgc gatgtcggtc
     1921 gccagttgct cctgccgctt caccgagccg gcgccctgac tgttggatga ggacgatccc
     1981 ttcaccagcg cctccggctt gacattctcg tcgaacacct tgctgaactt ggtttcgtag
     2041 ccaaacagcc cctcgacgcc gaggttctcg ccgttctggc gcgaacgctg ggtctggttg
     2101 tcgatcttgg cgctgtccgt gatccgcacc ttgacggtga gcaggtcgcc gatctggctg
     2161 gcgcgctgat ccttgaaaaa ggcacgcgat ccggtgcgcc acagcgagtt cgacgcatgc
     2221 acgctgggct gaggcatcgg catgggcatc tgcaccggct tgtagccggg ctgggcgacc
     2281 gggttctcga tggcgctcaa cggagggggc tggccgatgt tcgcgatgcg gtcgatgctg
     2341 ccgcaggcgg tcagcggcat cgccgccatc agggccagtc ctttccaggg aaaccggggg
     2401 acacaacagg acttcggcat tggcttactc ggcagggtga tggcgggcgc tgacgcgacc
     2461 cggcccgatg acgatggtct cgatcgtgcg cttggattgc aggttctgaa ccgcgacgat
     2521 ggcgccctcg gcgccggccg ccagggcctt tccgcgcagc gacagcgtca ttccggccga
     2581 ttcgtagagc acggtgacga gctggttgcg ttccaccatg tctggacgga cgacgtcccc
     2641 gtcccgtagc ggctggccaa cgcgcaaggc cttgcgggcg acgaacccgg tcaggcgcgc
     2701 gggttcggtc aaggcgccgg ccgggatgtc gcgccggcga cgctgttcaa gccgcaggtc
     2761 cgacgcctgg atcgtttcgc cgcgcgggat gtcgcgtgtc ggaaccggga gcgacagccg
     2821 cgtgtcgtag cttccggaaa gacggaccgg ctctcccggc tgatccggaa gcacgagatg
     2881 ggcctcgaac cgctccagtc tgcgatccag cgtcaccacc tcggtacgga tctcggcggt
     2941 gttgattgcg tcacgcgcga gttgcggagg cggggtgtcg aaccgcagcg tcgcttcggt
     3001 gaggctggtg tcgctgcgcc ggatggcttc cagcacgctc tgctcgatct cctgtgcgct
     3061 gaccagacgg gaggggcgcc gcacgacgac ggccgtcagg ccacgtgcgt cgatcgtttc
     3121 cagaccggcc tcgcgtccgg cctcgatgat ccgttctgcg ctgatcatgc cgctcttgcc
     3181 gaggtccggg gcgcggaaca gtggcgtggc ggccaaggct ccggcatgct cgacgatgtc
     3241 gccgaggcgc acgacgcctc cctggatgac ggccgccggc ttcagtgcgg gcgtctgggc
     3301 aaaggccccc gcgcaggcaa gggcgcagac catggcgatg atcgagcgtc tcatgttcac
     3361 tccctgctag ccgcggaagg cctgtgaggt ggcactcagc atctggtctg cggcctggac
     3421 caccttggcg ttgagttcgt aggcgcgctg ggcggcgatc agggcggaga tttccgtcac
     3481 ggcattgacg ttcgattcct caaggtagcc ttgctgaagc gtgccgaagc catccgcgtt
     3541 cggattgccc gtgatggctg tgccggaccc ctgggtttcg acgaagaggt tgtcgccgat
     3601 ggattcgagg cctgtcttgt tgacgaaacg ggcgagctgg atctggccca gctgctgggg
     3661 ctgcacctgg cccggaaggg aagcagacac cacgccctga gcgttgatgc tgatgccctt
     3721 ggccgtattg ggaaccgtga tcgccgggtc gagcaggtag ccctctgccg tcaccatgcg
     3781 tccatccgcg tcgagctcga acgagccgtc gcgggtgtat cccgtgcggc cgtccggcag
     3841 ttggacgcgg aagtagcctt cgccccggat ggcgaggtca taggactttt ccgttggcga
     3901 caggttgccc tggcccatga tgcggggcgt cgacacggac ttgacacccg agcccacata
     3961 gatgccggcg gggaccgatg tgccctgatc ggaactcggg ctgccggcgc gcttgtgcgt
     4021 ctcgtaaagc aggtcctgga actgcacccg catgcgcttg aagccggtgg tgcgcatgtt
     4081 ggcgacgttg ttggagatga cctcgacgtt gagttcctgg gccatcatgc cggtggcggc
     4141 ggtttgcagg gcgcgcatgg tgtcctcagg ctgcttcggc gagcttggtg atggcggagc
     4201 ggcgcaggtc gtcaccgcgc tgcatgctct gggcgagcga ggtataggca cggctgactt
     4261 cgatcatgcg cgagatttcg accaccgggc tgacgttgga cttttcgagg acgcccgatt
     4321 cgaggcgaat ttcggcgctg gccgggcggg gcgcctcgcg tgccgagaac agattgcccc
     4381 cgatgttctc cagcaccgac ggccgcgtga acgccgcgag cttcaggcga cctctcacgc
     4441 cctgcgcggt ggagacggtt ccgtctgcgg cgatcgcgat gtcccggtcc gctgcggtga
     4501 aagccagcgg gccctgttcg gcgagaaccg cgtggccatc gctcgtcacc agttcgccgc
     4561 gcgcgtcgag cgagagcgcg ccgttgcggg tatagcgttc gccgcttgac gtgcggacca
     4621 cgaagaacgc atcgcccttg atcgtcacgt ccagcgggtt gccggtgcgg tccgcagcac
     4681 cctgcgacag gtccagcgtc gacccccggt cgatgacaaa ggagatcgcg cggtctgcgg
     4741 gacgcaagct gtcggcccgc gcgcgcggcg ccacgtgttc gttgaacatg ccggatcggc
     4801 gcttgaaacc gttggttgct gaattggcga cattatttgc gatcacatcc atcgcacgtt
     4861 gcagggctat gtgccgcgag aggctgatgg gagcgatgtt ctccatgtcc gttcctaccg
     4921 ttccgatcat tgggcgtgac gagcgagaca gaggccagca atgcccgtgc cagcatgcag
     4981 ttcgtgattt attccaatga ttacagctat ttgtctggag cctgatagtc caggtcccga
     5041 tcttcgtcgt ggcacttccg gacggtggcg gcaaaatctg ccgggttcgg gcgtcggtaa
     5101 ccatttgcta accaatgcgc ggcaaggttc gaaaaacacg cacaggttgt atttatggcg
     5161 aagtcgaaga agggtgatga caagcaggcg gacggcgaga gcccgccgaa ggggcgcaag
     5221 ctgctgctga tcggcgccgc ggtggtcgtg ctcgcggccg ggggcggcgg cttctggtgg
     5281 ttcaagctga agccgtccgg cgccaaggcc gagatgaaga aggtcgcggt cttcctcgac
     5341 ctgcccgaca tgacggtcaa cctggcgcag ggcgctggca acgagcgcca gagcttcctc
     5401 aagctcaagg tttcattgga ggtcgcggat cagaagacgc tggccgaaat tcagcctgtg
     5461 atgccgcgcc ttctcgacaa tttccaggtc ttcctgcgcg agctgaaggt ctctgacctc
     5521 gaagggtccg ccgggctcta tcgcctgaag gaagaactgg tgcgccgcac caacatcgcg
     5581 gtgcatccgg ccaaggtgga agcggtgctg ttcaaggaca ttctggtgca atagggcagg
     5641 caatgcagga cttttccggc aataccgtac gcgcggccga agtttcggct ccctcgactg
     5701 tccccgttcc cggcagtcag ccgaccgcga ggccggaaca ggccggccgc gccggtgtcg
     5761 cgttgacgga tatttgcggc cggggcgagc gggcgccggt cgttctgccg atgctcgacc
     5821 tggcgctcga ccgggtgccg gacatcctcg ggcgacgcct gtccggggcc tggaacgggc
     5881 gggttaccgc cgcagcttcc ggtcgccgcg tcgcgcggtt cgatgcgctg gaagcagagc
     5941 tgaatggtct caggctggtc gggctgctcc gccatgcgga gaacccggat ttcggtctgg
     6001 tcgttttccc ggcgcagagc gtttggcggc tcatcgattg catgctcggc ggaagcggcg
     6061 gcgatctggt ggatcgtcag cggttttccc atcttgagcg atgcctcatg cgtgcgccgg
     6121 tggatcagct tctggctggt ctgggcgagg ctttcgaacc ggtcgcacca atcggattcg
     6181 acttcgtcgg ctgggcggaa ggcttggacg ccgccaggcc cggccgcatc ggtgatccgg
     6241 ttctaatgca ggaactgaca atcacgctgc ccagggggga cgccaggttc gatctgctgc
     6301 tgccgctcag cctgttcgag cggcatgtgg aagcgctggc cgcaccgcat ccgggtgaac
     6361 gcctcgggca agacagtctc tggaaggaca gccttctggc cgaagtgcgg cgtgccaccg
     6421 tcgagatcga agcggttctc tatgaaggaa cggcgccggt tggacgaatg cgggcgttgc
     6481 agcccggcga cagctacgcc ttcggcctgc cgccgcagac actgctgcgt cttcgcgccg
     6541 gcggtgtcga tctggcgctg ggccagctcg gtcgttctgg ctcgctcgcc gccgtccggt
     6601 tcgagacgcc ggtcgaagcg acgacggagg ccaggtcatg atggccgggc tctccgtggg
     6661 gctgatcatc gaggcgctgg tcgccctcct gctggccgtg tcgatcggct actgcgtcgt
     6721 gcttgaccgg cggctccagc gcatccgcct caatgaggat gagatgcgcc gcaccatcct
     6781 tgaacttggg gcggcgaccg agcgcgcgga gcgcgcgatc gagaccctgc gtgatgccgt
     6841 gcatgattgt gaccgtacgc tggcggacag gctgcgccag gccgaacggc agtctggcga
     6901 tctcgttcaa caaatacggg ccggaagcga ggtcctgtcc cggatcagcc gcatcgtcgc
     6961 ggttggaaag ggcggcgttg cggcatgacg gccaggcgac cgcgtccacg tcttctgccc
     7021 gtcgtcatcg cggcggcggc cagcttgctg gcgctgaaga cgctcggact ggtgacgcag
     7081 ggaggctatg tcctcgggcc atcagccgag cgcgagcgct tcgcgcgggc gctgaccgag
     7141 ccgcgccgca acttcgatcc cgatccagag ccgacgggcg caacgccggc caagaaggac
     7201 accaaggaac cggccaagga tgccgcggcg aaggacgctc ccgcgccggc caagcccgcc
     7261 gatccttcct cccagccgtt gccgccgcct gtgccggcgg cggaacggca ggttctggaa
     7321 aagctctcgg agcggcgcag gcaactggat gacagggcga aggaactcga tctgcgcgag
     7381 cagctgctca agtcggccga gcagaagatc gaggaaaggc tcggcgaact gaagggtggc
     7441 gaaaagcctg cggaggacgg tggtgcccca aaggacaagt cggacgcgcc cttcaagtcg
     7501 ctggtcacga tctatgaagc gatgaagccg cgcgatgccg cgaaagtctt cgagcggatg
     7561 gagccgcgtg cgctggcgcc gctggtctcc cagatgaacc ctcggaagtt ctcggaaatc
     7621 ctggcgcaga tgacaccgga gtccgcggaa aaactgaccc aggcgttgat gcgccgctcc
     7681 gacccggctc atggcggagc acgcagccag ccggctcact cgtccgaaac gccgaaaaag
     7741 cccgagctgg aagaattgcc gcggctggag cggcaaccga agggcacttg agaagagcgg
     7801 acgctacttt cctggcggca ccattcccgg agctgcctcc ggcgcctttc ccaggccctg
     7861 acctattgag ttcagggtgc tcgccagttt tccccccata gagtcaagcg ccgggatgac
     7921 ggcgagtgcc gaaagtgcgg cgatcagccc atactcgatg gctgtcgccc cgccttcgtc
     7981 gggcaggaac ctgacaagct gtcgccggat ggcgccgggc ggacgcacgg acgtgagggc
     8041 tttcgcacac ttcatggcgg caatctgcca aaaattccca aatccaaccc tatcggacgg
     8101 ccacaaatgc gatcactccg ggtgacgttc cgtttaccct gttgggaggt tcagcgtgtc
     8161 gtcaagggaa tggcaacgcc acggatgcaa cctctaatag agtaactcta ataaatgctt
     8221 cttgaagtag cgtgatgcgt ttgctggaaa tccggtcccg atggcttgcc cgcatgcagg
     8281 gcatggcggc ctgcgcggct ttcgtgctgg tcgcgctcac gacgtccatc cgggcgcagg
     8341 aaacgacggc gagcgtcagc gcgcgcgaca ctggcggatt cgggcgcata gagtttctgt
     8401 tctcgctgcc gaccggtttc accgcgcgac tttccggcag cgtgctcgtg gtcgagttcg
     8461 accggcccgt cgcgagcaat ccggagaaat tgccactcgc cctgccgggc tatcttgcgg
     8521 cggcgcggct ggaccccgag cgccggtcga tgcggttcgc gctcacgcgg ccggcgcgca
     8581 tcgatgtgaa ggaagctgcg gaacgggttt tcctcgacct gttgcccacg acctggaccg
     8641 gaccgccacc gcccatgccg gctgatgtcg tgcgcgaact cgcacggcgt gcccgggaag
     8701 cgcaagctgc cattcgcgcg gcggaaaccc gcaccgccgc ccaaatccgc cacgccgtgt
     8761 cggtccggct ggcgatgcgg gaggaagggc ctcgcatcgt gttcgagtct cccgaggcgg
     8821 gcgatgcccg gctggatcac aacggcagcg acctgcgcat ccggttcccg cccggcgccg
     8881 cactcaatga ggccgctctc cgcgcattgc tgagcgaact cgcgaccgac attcgcgtgg
     8941 aggatggacg agaggggccc gttgcattcc tccggctgcg ccagaacagg cagtcgcgca
     9001 cgctggctga agagggggcc ttctttgtcg acctcgtggc cgacgcgccg gctgccggtc
     9061 ctgtgccccg cgttgaagcg actgtccagg aggcgaaacc ggtgcccgcg ccgccgcctc
     9121 ggaaacgatg accaaggtgc tctatccca
//
LOCUS       DAOMXN010000088         9104 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_4031_length_9104_cov_10.599403, whole genome shotgun sequence.
ACCESSION   DAOMXN010000088 DAOMXN010000000
VERSION     DAOMXN010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 9104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9104
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4031_length_9104_cov_10.599403"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="PK812_07725"
     CDS             <1..431
                     /locus_tag="PK812_07725"
                     /inference="COORDINATES: protein motif:HMM:NF037628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HRJ69482.1"
                     /translation="FGQHLEETLAADVARARADDALAIDAALTPASASVSLLQEIERA
                     GPFGQGNPEPVFAFPALRLVDLFEVGQGHLKLRLQSGDGARLSAVAFRSAQAPLGAAL
                     QRAKGNSVHVAGHLAVDRWGGRESVELRLTDAAAVQVRRG"
     gene            555..629
                     /locus_tag="PK812_07730"
     tRNA            555..629
                     /locus_tag="PK812_07730"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:588..590,aa:Glu,seq:ttc)
     gene            1084..2334
                     /locus_tag="PK812_07735"
     CDS             1084..2334
                     /locus_tag="PK812_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit beta family protein"
                     /protein_id="HRJ69483.1"
                     /translation="MRFSAFSVIEQGLKGNRGWPEQWRSPEPRASYDAVIVGAGGHGL
                     ATAYYLAKEHSMRNIAVVEKGWLGGGNTGRNTTVVRSNYLWEESEAIYEHAMKLWEGL
                     SHELNYNVMYSPRGVMMLAHNIHDEQVLKRHVHANRLAGIDSEWLTPEQAKAFCPLLN
                     IEKNVRYPVTGATLQRRGGTARHDAVAWGYARGADRLGVDIIQNCEVTGIRRDASGAV
                     SGVETAKGFIATRRIGVVAAGNTSVLMGMADVRMPLESYPLQALVSEPVKPIMSCVAM
                     SNTVHAYMSQSDKGELVIGAGTDVYTSYSQTGALHIANHTLEAICELFPAVRRLRMLR
                     NWGGIVDVTPDRSPIIGKTPVPGLFVNCGWGTGGFKATPGSGHVFAHLVAKGEPEAIA
                     APFSLDRFRTGRLIDEAAAAAVAH"
     gene            2345..2635
                     /locus_tag="PK812_07740"
     CDS             2345..2635
                     /locus_tag="PK812_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018431419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HRJ69484.1"
                     /translation="MLLIHCPYCEMERPEIEFRYAGEAHLARPDNPDDATWAEFLYLR
                     TNPRGMHAERWRHIHGCGRYFNCLRDTVSDRIAETYKAGEARPTTLSGKGAA"
     gene            2632..5601
                     /locus_tag="PK812_07745"
     CDS             2632..5601
                     /locus_tag="PK812_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit alpha family protein"
                     /protein_id="HRJ69485.1"
                     /translation="MSEAFRLPGADGCPIRFVFDGMPYQGREGDTLASALLANGVHLV
                     GRSYKYHRPRGILTAGAEEPNALVAMMRGPGRMTPNLRATQIEIFDGLEVRSQNAWPS
                     LALDAGAINDIAGKFFSAGFYYKTFMGPKVAGSGLWTRVFEPIVRQAAGLGARTSEPD
                     PDRYAQFHEHCDVLICGGGPAGIAAALAAGASGKDVVLCDEQAAFGGGLLHETRATID
                     GKPTRQWLAEALAALRAMPNVRLMPRTQAFGYYAQNFIALSERVADHLATDHPVRERL
                     WQVRAKEVVIATGAIERPLVFSGNDRPGVMLAEAARIYLNRYGVKVGTRVVLVTAHDS
                     AYRAALDLKAAGVTIAAIADLRSDPAGELVEAARAAGLPLIADARVAGTKGRLRVSKV
                     GIAAGVGATRWIGCDCLLMSGGWTPSVHLYSQSRGKVVWHEDRQMFLPGASVQAERSA
                     GACKGTFALATVLAEGQQAGAAAAGTSSPSITYVVAGDPPQSGGHLGRVKAADGASGK
                     AFVDFQNDVKADDIDLAVQEGMRSIEHVKRYTTTGMATDQGKTSNLNALAIAAEKLGK
                     SVPEVGLTTFRQPYTPVSFGAFAGANRGELFDPLRRTPTHAWAEESGAVFEDFGLWKR
                     ARYFPRAGEDMHAAVHRECTQARKSAGVFDASTLGKIEVVGPDSAAFLEMLYTNPWAK
                     LEPGRCRYGLMLNEAGFVMDDGVIARLAADRFHVTTTTGGAPRVLHHMEDYLQTEFPD
                     LRVWLTSTTEQWAVIAVQGPKAREIVAPLVEGFDIAHEAMPHMSIREGRVCGIPARVM
                     RVSFTGELGYEINVPADFGRSVWEAVWAEGRKHDAVAYGTEAMHVMRAEKGHIIVGQE
                     TDGTVTPDDLCLGWAIGKAKKDFIGKRSLVRPDMVAPGRKQLVGLLTVNPETVLEEGA
                     QITATATPKVGEAAQGHVTSAYDSPALGRSIALALVADGRKRKGQTLHVPMPGGAIAV
                     TVVDPVFFDPKGDRLNV"
     gene            5594..6151
                     /locus_tag="PK812_07750"
     CDS             5594..6151
                     /locus_tag="PK812_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008598250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit gamma family protein"
                     /protein_id="HRJ69486.1"
                     /translation="MSDRMLASRRGVLDGLFLPKGEEFSLTPAPDAARFTLRGSPQAA
                     RAFGPAPPQVLRATSKGERLALWLGPDEILLIAPGEDAGAVQTELEHKLAGEPHALVD
                     VSHRQIGLVLEGRLAARCLSAGCPLDLRLSAFPVGMATRTIFLKAEIVLFRQEDQRFH
                     VEVWRSFAPYLIGHLGEALIGATGL"
     gene            complement(6298..7611)
                     /locus_tag="PK812_07755"
     CDS             complement(6298..7611)
                     /locus_tag="PK812_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HRJ69487.1"
                     /translation="MVSASSQISPSAHGRRIAVIGLGYVGLPVAVAFGRSGSPTIGFD
                     VNPRRIAELKAGKDSTCEVEEAELAEAKIAYSDQPDVLREADFFIVTVPTPIDAARRP
                     DLTALGKASATIGKALRKGGIVVYESTVYPGATEEFCVPILEQSSGLKAGVDFTVGYS
                     PERINPGDKNHRFETIRKVVSAQDAATLRIVSAVYGSVVKAGIFEAQSIKVAEAAKVI
                     ENTQRDLNIALMNELSCIFELLDIDTASVLAAAGTKWNFMPFTPGLVGGHCIGVDPYY
                     LTHRAEIAGYHPEVILAGRRINDGVGARIAKECVRRLMRSSVSRPVVTVLGLTFKEDV
                     PDVRNSKVIDVIAGLKAAGVTVQIHDAHADPDEVRHEYAVDLTAMSALEPADAVILAV
                     PHREYLADGWKLVRDRLNGGKGIVFDVKARLDLASQPDGIELWRM"
     gene            7840..>9104
                     /gene="asnB"
                     /locus_tag="PK812_07760"
     CDS             7840..>9104
                     /gene="asnB"
                     /locus_tag="PK812_07760"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HRJ69488.1"
                     /translation="MCGITGFIDVSRSLDAQALEGIARDMAGAMPHRGPDDDGTLAVA
                     EHGVAFGFRRLAIIDLTPAGHQPMTSGDGRWSIVYNGEIYNAEDMRPPLVARGVRFRG
                     HSDTEVLLESFSAFGVEATLTGAIGMFAMAVHDRERGETWFARDRLGKKPLYIGRFGK
                     TFMFGSELRCLRAHPDFRAELDETAIASYARFGYIPHPLCIYRGVTQLAPGGLARLSR
                     EGDLKVGHYWDPAGAAANAKQQGFSGSPEEAVEELDRLLRDAVGRRMVSDVPLGAFLS
                     GGIDSSVVVALMQAQSARPVKTFSIGFHVDGFDEAPHAKAVATHLGTEHHELYVQPQD
                     ALDVVPMLPTMYDEPFSDSSQIPTYLVSRMAREHVTVALSGDGGDESFAGYTRYRQIG
                     ALFGDQGPASGAATLLARLAGGLSAPLGRR"
BASE COUNT         1537 a         2948 c         3011 g         1608 t
ORIGIN      
        1 atttcggcca gcaccttgag gagacgctcg ccgccgatgt cgcccgcgcc agggcggacg
       61 atgcgctcgc catcgatgca gcgctgacgc cggcttcggc gagcgtgtca ctcctgcagg
      121 agatcgagcg cgccggcccg ttcggacagg gcaatccgga gccggtcttc gcctttccgg
      181 cgctgcgcct ggtcgatctg ttcgaggttg gtcaggggca cctcaaactg cgcctccagt
      241 cgggcgatgg cgcgcgactc tccgccgttg cctttcgctc ggcgcaggca ccgctgggcg
      301 cggcgctgca aagggcgaag gggaattcgg tgcatgtcgc aggccatctg gcggtggacc
      361 gctggggcgg acgggaaagc gtggaactgc gcctcaccga cgccgccgcg gtccaggtca
      421 ggcggggctg aaggggcagc cgggcgcacg cgacaggggt gctccgctcc ctcgaaaatc
      481 gcttccattc aggcttgccc gaccggcgcc ctgaggctat aggggcgtct cgccgctgca
      541 gcgcggtcgg ccgcgcgccc ttcgtctatc ggttaggacc ccagattttc aatctggtaa
      601 gacgggttcg actcccgtag ggcgcgccat cgcttctata ccgccggaat ccagccctaa
      661 caccttgaac ggcaaggtgg tttttggggt acgaaacccg tccttcggac agtacgccag
      721 acgcccggca atggtcagcc gcagcacgag cctgcgatag tccggtttcc gcaaaacacc
      781 agatttttga agggcttgcg aggaactgca tggcgcgttc gaacacctcc tcaaaggtgc
      841 catgccgctg ggtgcttgca gcgcgccttt cgtcagcggg gatgaccgtg gcggacgtgg
      901 ctaccgggac aagacttgcc gtggcgccgg caagctctcg acgtccaagt ccgtgccgcg
      961 agggaccggc gggctttccc actgcccggc atgtcggaat aatgctgggt atctgccgga
     1021 aaactcctcg atttcggtcg ccgcctttgc cactcttgct gaacaagttg ggggtgcgtc
     1081 gagatgaggt tttccgcttt ttccgtcatc gagcagggcc tgaagggcaa tcgcggttgg
     1141 cccgagcaat ggcgctcgcc ggaacccagg gcgtcctatg acgccgtcat tgtcggcgcg
     1201 ggcgggcatg gcctcgccac cgcctactac ctggcgaaag agcacagcat gcgcaacatc
     1261 gccgttgttg agaagggctg gctcggcggc gggaataccg gccgcaacac gacggtggtg
     1321 cgctccaatt atctttggga ggaatccgag gcgatctacg aacatgcgat gaagctgtgg
     1381 gagggcttga gccacgagct caactacaac gtcatgtact cgccccgtgg tgtcatgatg
     1441 ctggcgcaca acatccacga tgaacaggtg ctcaagcgcc atgtccatgc gaaccggctc
     1501 gccggcatcg acagtgaatg gctgaccccg gagcaggcga aggcattctg ccccctgctg
     1561 aatatcgaga agaatgtacg ctacccggtc accggcgcca cgctccagcg gcgcggcgga
     1621 acggcgcggc acgatgctgt tgcgtggggc tatgcgcgcg gcgcggatcg ccttggcgtc
     1681 gacatcatcc agaactgcga ggtgacggga atccgccgcg acgcgtcggg cgcggtttcg
     1741 ggcgtcgaga cggcgaaggg attcatcgcc acacggcgca tcggcgtggt cgcggccggc
     1801 aacacttcgg tgctgatggg catggcggac gtgcgcatgc cgctggagag ctatcccctg
     1861 caggcgctgg tgtcggaacc ggtcaagccg atcatgtctt gcgtcgccat gtcgaacacg
     1921 gtgcacgcct acatgtcgca atccgacaag ggcgaactcg tcatcggcgc gggcacggac
     1981 gtctacacgt cgtattctca aaccggggcc cttcacatcg cgaaccatac gctggaagcg
     2041 atctgcgagc tgtttccggc ggtgcggcgt ctcaggatgt tgcgcaactg gggcggcatc
     2101 gtcgacgtca cgcccgatcg ctcgccgatc atcggcaaga cgccagtgcc ggggctgttc
     2161 gtcaattgcg gctggggaac cggcggcttc aaggcgacgc ccgggtcggg tcatgtcttc
     2221 gcccatctgg tggcgaaagg cgaaccggag gctatcgcag cgccgttctc gctcgatcgc
     2281 ttccgaacgg gtcgcctgat cgatgaggcc gccgctgcgg ccgtagcgca ttgaggaggg
     2341 agcgatgctg ctgatccatt gcccctattg cgagatggaa cgccccgaaa tcgaattccg
     2401 ctatgccggc gaggcacatc ttgcccgccc ggataatccg gatgacgcga cctgggctga
     2461 gttcctctat ctccggacca atccacgcgg gatgcatgcc gagcgctggc gccacatcca
     2521 tggctgcggg cgctacttca attgcctgcg cgacacggtg agcgatcgca ttgcggaaac
     2581 ctacaaggcc ggtgaggcgc ggccgaccac cctgtccggt aaaggtgcgg catgagcgag
     2641 gccttccgcc ttcccggcgc cgatgggtgt ccgatacgct ttgtttttga cggtatgccc
     2701 tatcagggcc gtgagggcga tacgcttgcc tcggcgctgc tggccaatgg cgtgcatctc
     2761 gtcgggcgct cgtacaagta ccaccgcccg cgcggcatcc tgacggcagg cgcggaggaa
     2821 cccaacgcgc tggtggcgat gatgcgcggg ccggggcgca tgacgccgaa cctgcgcgcc
     2881 acgcagatcg aaattttcga tgggcttgag gtgcgcagcc agaacgcctg gccctcgctc
     2941 gccctcgatg cgggcgcgat caacgacatt gccggcaagt tcttttccgc cggcttctac
     3001 tacaagacgt tcatgggtcc gaaggtagcc gggagcgggc tttggaccag ggtcttcgaa
     3061 cccatcgtcc ggcaagcggc aggtcttggt gcgcgcacca gcgagcccga cccggatcgc
     3121 tatgcccagt tccacgagca ctgcgacgtg ctgatctgcg gtggcggacc ggcgggcatc
     3181 gcggcggctc tggcggcggg agccagcggc aaggacgtgg tcctgtgtga cgaacaggcc
     3241 gcgttcggtg gcggcctgct gcacgaaacc cgcgccacca ttgacggaaa gccaacacgc
     3301 cagtggctgg ccgaggcgct tgccgcgctc agggccatgc cgaacgttcg gctgatgccg
     3361 cgcacgcagg cgttcggcta ttatgcccag aatttcatcg cattgtcaga gcgcgtggcg
     3421 gatcatctcg caaccgatca tccggtgcgg gagcggcttt ggcaggtgcg ggcgaaggag
     3481 gtcgtcattg ccaccggcgc catagaacgg ccgctggtgt tctccggcaa tgatcggccc
     3541 ggcgtgatgc tggcagaggc cgcgcggatc tatctcaacc ggtacggggt gaaggtcggc
     3601 acccgagtgg ttctggtcac ggcgcatgac agtgcctatc gcgcggcact cgatctcaag
     3661 gcggccggtg tcaccattgc cgccatcgcc gacctcagat ccgatccggc aggcgaactg
     3721 gtcgaggccg cgcgtgcggc agggctgccc ctgatcgctg atgcgcgcgt cgccggaacg
     3781 aagggccggc tgcgggtatc gaaggtcggc atcgccgcag gcgttggcgc aacccggtgg
     3841 atcggctgcg actgcctgct gatgtccggc ggctggacac cgagcgtgca tctttattcg
     3901 cagtcgcgcg gcaaggtcgt ctggcatgag gaccgccaga tgttcctgcc cggagcctcg
     3961 gtgcaggccg agcgatcggc gggggcctgc aagggcacgt tcgctcttgc cactgtgctg
     4021 gcagagggac aacaggccgg cgccgctgct gcggggacct cgagcccgtc catcacatac
     4081 gtcgtcgccg gtgatccgcc ccaatcgggt ggccatcttg gccgcgtcaa ggcggcggat
     4141 ggcgccagcg gcaaggcctt cgtcgatttc cagaacgacg tgaaggccga cgatatcgac
     4201 cttgccgtgc aggagggcat gcgctcgatc gaacacgtca agcgctacac cacgaccggc
     4261 atggctaccg atcagggcaa gacctcgaac ctgaatgcgc tcgccatcgc agccgaaaag
     4321 ctcggcaagt ccgttcccga ggtcggactg accacgttcc gccaacccta cacgccagtc
     4381 agcttcggag ccttcgccgg cgccaaccga ggcgagctgt tcgatccgct gcgccggacg
     4441 ccaacccacg catgggcgga agagagcgga gcggtgttcg aggacttcgg cctttggaag
     4501 cgcgcgcggt atttcccgcg cgccggcgag gacatgcacg cggcagttca ccgcgaatgc
     4561 acgcaagccc gcaagagcgc cggcgtcttc gacgcctcca ccctcggcaa gatcgaggtg
     4621 gtggggcctg attcggcggc gtttctggag atgctctaca ccaacccctg ggcgaagctc
     4681 gaacccggtc gctgccgcta cgggctgatg ctgaacgaag ccggcttcgt gatggatgac
     4741 ggcgtcatcg cccgtctggc ggcggaccgc ttccacgtca cgacgaccac cggcggcgca
     4801 ccccgcgtgc tgcatcacat ggaggattat ctccagaccg aattcccaga tctcagggtc
     4861 tggctcacct ccaccaccga acaatgggcg gtgatcgcgg tgcaggggcc gaaggcgcgc
     4921 gagattgttg cgccgctggt tgagggcttc gacattgccc acgaagccat gccgcacatg
     4981 tcgatccgcg aaggccgggt ctgcggcatc cccgcgcggg tcatgcgcgt cagcttcacc
     5041 ggcgaactcg gctacgagat caatgtgccg gccgattttg gccgctcggt ctgggaagcc
     5101 gtctgggccg aaggcaggaa gcacgacgct gtcgcctacg gcacggaagc catgcatgtg
     5161 atgcgcgccg aaaagggcca cattatcgtc gggcaggaaa cggacggcac ggtgacgccg
     5221 gacgatcttt gcctcggctg ggccatcggc aaggccaaga aggactttat cggcaagcgt
     5281 tctctggtcc gcccggacat ggtggcgccg ggccgcaagc agctcgtcgg attgctgacc
     5341 gtcaaccccg aaacggtgct ggaggagggg gcgcagatca ccgcgaccgc gacacccaag
     5401 gtgggcgagg ccgcgcaagg tcatgtcacg tccgcctatg acagcccggc gcttggccgg
     5461 tcgatcgcgc tggcgctggt ggccgatggt cgcaaacgca agggacagac cctgcacgtc
     5521 cccatgccgg gaggcgcgat tgccgtgacc gtggtcgatc ccgtgttttt cgatcccaag
     5581 ggagacaggc tcaatgtctg accggatgct cgccagccgc cgcggcgttc ttgacggcct
     5641 cttcctgccg aagggcgagg aattttccct gacgccggcc cccgatgccg cgcgcttcac
     5701 cctgcgcggc agtccgcagg ctgcaagagc ctttggcccg gcgcccccgc aggtgctgcg
     5761 cgcgacatcg aagggcgagc gcctggcgct ctggcttggt ccggacgaga tcctgctgat
     5821 cgcgccgggc gaggatgccg gggcggtcca aacagaactt gagcacaagc ttgcgggtga
     5881 gccgcatgcg ctggtcgatg tctcgcatcg gcagatcgga ctggtgctgg aagggcggct
     5941 cgcggcgcgc tgcctgtccg ccggttgccc gctcgatctc cgtctctccg ccttcccggt
     6001 cggcatggcc acgcggacga ttttcctcaa ggccgaaatc gtgctgtttc ggcaggagga
     6061 ccagcgcttc catgtcgagg tctggcgctc cttcgcgccc tatctgatcg ggcacctcgg
     6121 tgaggccttg attggcgcta caggtctttg aaacagttca agaagccgcc aacgccttca
     6181 tcgcggcgcc gaggccgcag cgtcgcatct gatgccttga cgccgagcat gttaggccgc
     6241 gcgccaaacc gcgtcggccg gccatgcgcg caatgccccg gccaggtcgg gccggctcta
     6301 catccgccag agttcaatcc cgtccggctg tgatgccaga tcaagccgtg ctttcacatc
     6361 gaagacgatg cctttcccgc cgttcagccg gtcgcggacg agcttccagc cgtcggcgag
     6421 atactcgcgg tgcggcacgg ccagaatcac ggcatctgcc ggctccagtg ccgacatggc
     6481 cgtcaggtcc accgcatact cgtgccggac ttcatccggg tccgcatggg cgtcgtggat
     6541 ttgcacggtc acgccagctg ccttcaggcc ggcgatcaca tcgatcacct tggagttgcg
     6601 cacatccggc acgtcttcct tgaaggtcag tccgagcacg gtcacgaccg ggcgcgaaac
     6661 cgaactgcgc atcagcctgc gaacgcattc cttggcgatc cgggcgccga caccgtcatt
     6721 gattcgccgg ccggcgagaa tgacctcagg atgatacccg gcgatctcgg cgcggtgcgt
     6781 cagatagtag gggtcgacgc cgatgcagtg accgccgacc agacctggcg tgaacggcat
     6841 gaaattccat ttggttcccg ccgccgcgag caccgaagcc gtgtcgatgt cgagcagttc
     6901 gaaaatgcac gacaattcgt tcatcagcgc gatgttgagg tcccgctgcg tgttctcgat
     6961 caccttcgcg gcctcggcca ccttgatgct ctgtgcctcg aaaatgccgg ccttgacgac
     7021 cgatccgtag acggccgaga caatccgcag cgtcgcggca tcctgagccg aaaccacctt
     7081 gcgaatggtc tcgaaacgat ggttcttgtc acccggattg atgcgctcgg ggctgtagcc
     7141 caccgtgaag tcgaccccgg ccttcagccc ggaactctgt tcgagaatcg gaacgcagaa
     7201 ttcctccgtc gcgccgggat agacggtcga ctcgtagacg acgatgccgc ccttgcgcag
     7261 cgccttgccg atggtggcgg atgctttgcc cagcgccgtc aggtccggtc gacgcgccgc
     7321 atcgattggc gtgggcacgg ttacgatgaa gaagtcggcc tcccggagga catccggctg
     7381 gtccgaatac gcaatcttcg cttccgcgag ttcggcctct tccacctcgc aggtcgagtc
     7441 cttgccggct ttcagttccg caatgcgccg cgggttgaca tcgaacccga ttgtcgggct
     7501 gccggagcgg ccgaacgcga ccgcgaccgg cagtccgacg tagccgagtc caatgacggc
     7561 aatgcggcgt ccatgcgcgg acggcgagat ctgagaagat gcactgacca taacgtttac
     7621 gaccaacttg gcttgtaggg cattgaaaag ctgcttgcct atgccggtcg ggccggaagc
     7681 aagtcaaccc ctgcaagcat gcgccgggcc atgcgtgccg cgagttgacc tgaactggca
     7741 tgcggcatag ggagctacca tgatccggat cgaacccgcc cgaattcgcc gttcttccga
     7801 tgcggcgccc ggcactctgg cagccagata ggccaaacca tgtgcgggat tacgggattc
     7861 atcgacgttt cgcgatctct ggatgcgcag gcgctggaag gcatcgcccg cgacatggcc
     7921 ggtgcgatgc cccaccgcgg cccggacgat gatggcacgc tggctgttgc cgagcacggc
     7981 gtcgcgttcg gatttcggcg tctggccatt atcgatctga cacccgcggg ccaccagccc
     8041 atgacatccg gcgacggacg ctggtccatt gtatacaacg gcgaaatcta caacgccgag
     8101 gacatgcgcc cgccgctcgt cgcccgtggc gtccgtttcc gcgggcattc cgacaccgag
     8161 gtgctgctgg agagcttctc ggcattcggc gtcgaggcga cccttacggg cgcgatcggc
     8221 atgttcgcga tggctgtgca tgaccgggag cggggggaaa cctggttcgc gcgtgatcgc
     8281 ctgggcaaga agccgctcta tatcgggcgc ttcggcaaga ctttcatgtt cggttcggag
     8341 ctgcgctgcc tgcgcgccca tcccgacttt cgcgccgaac tcgacgaaac ggccatcgcg
     8401 tcctacgccc gcttcggcta catcccccat ccgctgtgca tctatcgcgg cgtgacccag
     8461 ctggcgccgg gaggcctggc gcgtctgtcc cgcgagggtg acctcaaggt cggacactat
     8521 tgggatcccg cgggcgcagc cgccaacgcc aagcagcagg gcttctccgg ctcgccggag
     8581 gaagccgtcg aggagctcga caggctgctt cgcgatgccg tcggacgacg gatggtttcg
     8641 gatgtcccgt tgggcgcatt cctgtccggc ggtatcgatt cttccgtggt cgtggcgctc
     8701 atgcaggcgc aatccgcccg gccggtgaaa acgttctcca tcggcttcca tgtcgacggt
     8761 ttcgacgaag cgccgcatgc caaggcggtc gccacgcatt tgggcacgga gcatcatgag
     8821 ctctacgtcc agccccagga tgcgctcgac gtcgtgccga tgctgcccac gatgtacgac
     8881 gaaccgtttt cggattcgtc acagataccg acctacctcg tatcccgcat ggcccgggag
     8941 cacgtcacgg tggcgctttc cggcgacggc ggcgacgaat ccttcgcggg ctatacgcgg
     9001 taccgccaga tcggggcgct gttcggcgat caggggcctg catcgggagc ggccacgctg
     9061 cttgcgcgcc tggccggagg cctgtcggcg ccgctgggtc ggcg
//
LOCUS       DAOMXN010000089         8982 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_4109_length_8982_cov_11.181136, whole genome shotgun sequence.
ACCESSION   DAOMXN010000089 DAOMXN010000000
VERSION     DAOMXN010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 8982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8982
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4109_length_8982_cov_11.181136"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..828)
                     /locus_tag="PK812_07765"
     CDS             complement(19..828)
                     /locus_tag="PK812_07765"
                     /inference="COORDINATES: protein motif:HMM:NF019829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaX family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HRJ69489.1"
                     /translation="MLDQPHHPALARPLAAFHARTPPRIWSLIITFLGDAILPRGGLV
                     RAGVISDFCSLADVEAGLVRTALSRLVAKGFVQRSREGRASFYRLTEAERRDFGSAAD
                     LIYGRRLPQPDGGWDLALLDTLDGSTALRTRIASLRYRAIAANVYMRPHHREDAAFGE
                     PELTILDARPRSDIGPLAARLWPVAEIAASYDLFVRNFDGIATARLDSAESILARILL
                     VHEFRRVLLRDPFLPVEALPAPWPGSAARRLFDTAHAALTPMAEAWLDAHG"
     gene            complement(859..1059)
                     /locus_tag="PK812_07770"
     CDS             complement(859..1059)
                     /locus_tag="PK812_07770"
                     /inference="COORDINATES: protein motif:HMM:NF018630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HRJ69490.1"
                     /translation="MQHVSTESPCIGACRLDPQGRACLGCGRTLREITRWRDLSDEER
                     RRVNARLAGFVPGGGSAAPRRS"
     gene            1132..1884
                     /locus_tag="PK812_07775"
     CDS             1132..1884
                     /locus_tag="PK812_07775"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRJ69491.1"
                     /translation="MATLRAGDKRFEFDLVVFDKDGTLVDFDRLWAFKMRRAIEVLVK
                     ETGLDASSTARLLRLIGVDPETLKVVPESPLAVTTLPKIGVVATVGLHQAGFAWHAAE
                     AVVHRVFMPAIEASPAPEDLEPIGPVRPLMQAIAAQGARIAILTSDDRRATEESLPLL
                     GIDALVDAMVCGDDPLPSKPAPDGLLKLARDLQVPPGRVLMIGDSAADMRTGRNAGVA
                     GCIAVLSGTGRREDLAALADVVITDISDIGIA"
     gene            complement(1902..3425)
                     /locus_tag="PK812_07780"
     CDS             complement(1902..3425)
                     /locus_tag="PK812_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HRJ69492.1"
                     /translation="MSAATLAWFARHEARLAWRDWLAMATAGKPGRLRWIALALVVFA
                     AILHRIADRLITPYVDSIGSDVATFLVMTGSAGLTWTLMLSQGMESVTRAFYSRSDLD
                     LILSSPASGRTVFGLRIGATAFGTALLAVLLVGPFVNILAWRFGPQMLAAYGVVVALA
                     LLSTAFAVLLTTGLFRLLGAKRTRLVAQVVAAVVGAGFVIGVQAFAIVSYGNLSRVAV
                     FLAPEWVEAAPGIESMLWLPARAALGDIGLMLALLTVAFTVLALTIAATAGRFADNVI
                     AASGISHLRTVQRASGLDFRPLSVARALRHKEWLLLRRDPWLVSQSLMQLLYLLPPAL
                     LLWRKFGDKADALVVLAPVIVMAAGQLAGGLAWLAISGEDAPELVATAPVTPGAVTRA
                     KIEAVLGVVALAVLPLVVALAVASPFIALVTLLACGMAAAGATAVQFFFRAQARRSQF
                     RRRQTSSRFATFAEAFVSISIAAAAGLAAAENWQAIFALLFAGLILLAAYRSGSRQR"
     gene            complement(3422..4168)
                     /locus_tag="PK812_07785"
     CDS             complement(3422..4168)
                     /locus_tag="PK812_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ69493.1"
                     /translation="MADALSIRNLAKSFDRPAVDGLDLTVRAGEFYALLGPNGAGKTT
                     TLRMVAGLLRPDRGDIAIFGVDALRLPVEAKRITAWVSDEPMIYDKLTPFEYLAFVAG
                     LWQVEPAIAQARSQELIAVLGLEPHAHERCEGFSKGMRQKVALAGALVHEPRLIILDE
                     PLTGLDAGSARQVKDVLLQRVRSGATIIMTTHILEVAERMAERIGVIARGRLVAEGTL
                     EELRSHVGESGSSLEDIFLEIVRAPQDLAA"
     gene            complement(4274..5155)
                     /locus_tag="PK812_07790"
     CDS             complement(4274..5155)
                     /locus_tag="PK812_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRJ69494.1"
                     /translation="MHLLDIDQLRSLIAIADTGSFTRAAEKVFKTQSAVSMQMKRLEE
                     QLGRPIFHKEGRGARLNEDGDRLLSYARRIVGLSSECVATFSDAVLSGRVRLGVPDDY
                     ADRYLPEILARFTRSNPRVEVTVVCEPTPMLVDRLLGGDIDLAIVTLSKTLESGEVVR
                     HERLLWVTSARHSVHEETPLPLALGRPTCGWRQAATERLDAVGRSYRVLYASWNSSAV
                     GAAVLSGLAVGVLPESAIRPGMRVLLPSEGFAPLPEVRIALMRSKHADASIVDALSTQ
                     IIDSLDNLAQVRAAAAE"
     gene            complement(5647..6375)
                     /locus_tag="PK812_07795"
     CDS             complement(5647..6375)
                     /locus_tag="PK812_07795"
                     /inference="COORDINATES: protein motif:HMM:NF017853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HRJ69495.1"
                     /translation="MMNLADILANAHGGAAFQSLARQYGLSTEQAEAAVNAMLPAFSL
                     GLQQQAQSADGLINLMSMLGSGTGEAAFEDPTAAASKQMLNSGKEFMTQIFGGTQQAS
                     KEVQAQVVQQAAALTGIGNTVLQKMLPMIASIILGGLFKGAMNNGLGGIIEQVLKGGL
                     GQATAPRQGQDPMGDLLGGILGQVLGGLTGGQARPQPQQQADPITSGIDILKGMFETG
                     QTVQEAQMSGLQNIFDEILKAQKR"
     gene            complement(6452..7828)
                     /locus_tag="PK812_07800"
     CDS             complement(6452..7828)
                     /locus_tag="PK812_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRJ69496.1"
                     /translation="MNDASKPSPPDTRPAPAGKLTQGSTMRHVVVMTVTGAVGLVSIF
                     LVDFLSLFYISLLRNEEWTAGVGYATTFMFFAIAFNIGLMIAGTALVSRALGARDVDH
                     ARRVAGSSAVLLTGASLAVTLVMLAGMPWILDALGASGQPRAIAERFLWISMPSNPLM
                     ALGMAFSGVLRAAGDARRAMMVTLMGGIVTAVLDPLFIFALRLGTDGAALATVFSRLT
                     FCAIGFMGVVKVHRLMARPTLDDLSQNLRPLLYIAAPAVLTNIASPVAAGFMIGVVRQ
                     FGHEAVAASTIIDRLVPLAFGVIFALSGSVGPILGQNLGARHYDRVRRTLRDSLVFAA
                     SYSLFAWAMLALGRHQIAALFGASEKTAAYVGFFCLIGAGAWVFNGLLFVANAAFNNL
                     GFPLYSTFFNWGRATLGTMPFSYFGAQLWGYEGAIGLVIIGWGVFGTWSVIEAFRAVR
                     RIESQKTS"
     gene            7986..8963
                     /gene="ispH"
                     /locus_tag="PK812_07805"
                     /gene_synonym="lytB"
     CDS             7986..8963
                     /gene="ispH"
                     /locus_tag="PK812_07805"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HRJ69497.1"
                     /translation="MMKKPPLTILLCAPRGFCAGVVRAIDAVEEALRRFGRPVYVRHE
                     IVHNRYVVEDLKRKGAVFVDELHEIPDTQAPVIFSAHGVPKSVPADAERRNLMAIDAT
                     CPLVTKVHREAEIHHRRGRKVVLIGHAGHPEVVGTMGQLPEGAVTLIETLDDVGRLAD
                     PGDAPLAYVSQTTLSVDDTRGIVEALRARFPAIHGPNKEDICYATTNRQEAVKRVAPQ
                     VDALIVVGSENSSNSQRLREVAERAGCSVTRLLLRAEDIDWAAFGAIRSLGITAGASA
                     PEVLVEEIIDAFAARFDVTVETVSTADESVFFPLPRKLRDGDGPLTRSD"
BASE COUNT         1755 a         2998 c         2851 g         1378 t
ORIGIN      
        1 ccactggccg cctgaagcct agccgtgcgc gtccagccag gcctcggcca tcggcgtcag
       61 tgccgcatgg gccgtgtcga acaggcgccg agcggcgctg cctggccagg gggcggggag
      121 cgcctcgacc ggcaggaatg gatcgcgcag gagcacgcgt cggaactcgt ggaccagcag
      181 gatgcgggcg aggatgctct ctgcggaatc cagacgcgcc gtggcaatgc cgtcgaaatt
      241 ccgcacgaac aggtcatagg aagccgcgat ctccgccacc ggccataatc tcgccgcgag
      301 aggcccaatg tccgagcgcg gacgcgcatc gaggatggtg agttcgggtt caccgaacgc
      361 cgcgtcctcc cgatggtgcg ggcgcatata gacatttgct gcgatggccc ggtagcgcag
      421 gctggcaatc ctggtccgga gcgcagtgga gccatccaat gtatccaaca gggcgaggtc
      481 ccaccctccg tccggctggg gcaaccttcg gccatagatg agatcggcgg ctgagccaaa
      541 gtcccggcgc tcggcctccg tcaggcgata gaaggatgcg cgcccttcgc ggcttcgctg
      601 gacaaagccc ttcgcgacca gccgtgacaa ggccgttcgc accagaccgg cctccacatc
      661 ggccaggcta cagaagtcgg aaatcacgcc ggcgcgcaca aggcccccgc gcggtaggat
      721 cgcatccccc agaaaggtga tgataagcga ccatatgcgc ggcggcgtac gcgcatgaaa
      781 agcggcgaga ggccgcgcaa gcgcaggatg atgcggctga tcgagcatca aaccggccta
      841 agcccggccg ccgccaagtc aagagcgccg tggtgcggcc gaaccgcctc cagggacaaa
      901 accggcaagc cgggcgttga cgcgacggcg ttcctcatca ctaaggtccc gccaccgcgt
      961 gatttcgcgc agcgtccgcc cgcagccgag acaggcacgc ccctgcggat cgagccggca
     1021 ggcgccgatg cacgggcttt cggtggaaac gtgctgcatg acctactcgg gattgtttcg
     1081 acacgcgccc gctgctaccg cacgctagac caacagggca agcgaggcag catggcgacc
     1141 ctccgcgcgg gtgacaagcg gttcgaattc gatcttgttg tcttcgacaa ggatggcact
     1201 ctggtcgact tcgaccgcct ctgggcgttc aagatgcgcc gcgccatcga ggttctagtc
     1261 aaggagaccg gtctggacgc ttcatccact gcccgcctac tgcggctgat cggcgtcgat
     1321 cccgagacgc tgaaggtggt gccggagagc ccgttggccg taacgacctt gcccaagatc
     1381 ggcgtggtgg ccacggttgg cctgcatcag gcaggatttg cctggcatgc ggcggaagcg
     1441 gtagtgcatc gcgtgttcat gccggcgatc gaagcttctc ccgcgccgga ggacctcgag
     1501 ccgatcggtc ccgtccgtcc gctgatgcag gccatcgccg cgcagggcgc ccggatcgcc
     1561 atcctgacga gcgacgaccg acgcgcgacg gaggagtccc tgccgttgct aggcatcgac
     1621 gcgcttgtcg acgcgatggt ctgcggtgac gatccgctgc cgagcaagcc cgcgcccgat
     1681 ggcttgctga aacttgcccg tgacctgcag gtaccgcccg ggcgcgtgct gatgatcggc
     1741 gattccgccg ccgacatgcg caccggacgc aacgccggag tggcaggatg catcgccgtc
     1801 ctgtcgggca ctggccgacg cgaggatctg gcggcgctgg cagacgtcgt catcaccgac
     1861 atctcggaca tcggcatcgc ctgacgagcg gccgggcaaa cctatcgctg ccgtgagccc
     1921 gaccgatacg cggccagcaa gatcaggccc gcgaacagca gggcaaatat cgcctgccag
     1981 ttctcggctg ccgcgaggcc ggcagcggcg gcaatggaaa tcgacacgaa tgcctcggcg
     2041 aaggtcgcaa aacgtgacga cgtctggcgc ctgcggaact gggaccgtcg cgcctgcgcg
     2101 cggaagaaga actgcaccgc cgtcgcgccc gcggccgcca tgccgcaggc aagcagcgtc
     2161 accagcgcga tgaacggcga ggccacggcc agagccacga cgagcggcaa gacggcgagg
     2221 gccacgacac cgagcacggc ctcgatcttg gcccgcgtca ccgctccggg tgtgaccggc
     2281 gccgtcgcca ccagttcggg ggcatcctcg ccggagatcg ccagccaggc caggccaccg
     2341 gccagctgac cggccgccat cacaataacc ggagccagaa ccaccagggc gtctgccttg
     2401 tcaccgaact tgcgccagag cagcagcgcc ggaggcagca ggtacaagag ctgcatcagg
     2461 ctctggctga ccagccaggg atcgcgacgg agcagcagcc actccttgtg ccgcagggcg
     2521 cgcgccaccg agagaggcct gaagtcgagg ccgcttgcac gctgcacagt gcgcagatgc
     2581 gagatgccgg aagcggcgat gacattgtcg gcaaagcggc ccgccgtcgc ggcgattgtc
     2641 agggcgagaa ccgtgaaggc gacggtcaac agcgcgagca tcaacccgat gtcgccgagg
     2701 gcggcgcgtg cgggcaacca gagcatgctt tcgattcccg gcgcggcctc cacccattcc
     2761 ggcgccagga aaaccgccac ccgcgatagg ttcccgtagg agacgatggc gaaggcctga
     2821 acgccgatca cgaagccggc gccgacaacc gcagcgacga cctgcgcgac gagacgcgtg
     2881 cgtttggccc ccagcaggcg gaacaggcct gtcgtcaaca gcacggcgaa ggccgtggac
     2941 agcagcgcca gcgcgaccac cacgccatag gccgcgagca tctgcggccc gaagcgccag
     3001 gccagtatgt tgacgaacgg ccccacaagc agcacagcaa gaagcgcggt gccgaaggcc
     3061 gtcgcgccga tacgcaggcc gaaaaccgtg cgtcccgaag cgggcgaaga caagatcaga
     3121 tcgaggtcgg accgcgagta gaaggcgcgc gtgaccgatt ccatgccctg ggacagcatc
     3181 agcgtccagg tcaagccggc cgagccggtc atgacgagga aggtcgcgac atcgctgccg
     3241 atggaatcaa cgtagggtgt gatcaggcga tcggcgatgc gatgcaggat cgccgcgaac
     3301 accaccagcg cgagggcaat ccagcgcagg cgtcctggct tgccggccgt cgccatggcc
     3361 agccagtcgc gccaggccag gcgcgcctcg tgacgggcga accaggcgag cgtcgcggcg
     3421 ctcacgccgc caggtcctgc ggcgcgcgca cgatttcgag gaaaatgtcc tcaaggctgg
     3481 agccgctctc gccgacatgg gaccgcaact cctccagggt gccctcggca accagccggc
     3541 cgcgcgcaat cacgccgatg cgctccgcca tgcgctccgc cacttcgagg atatgcgtgg
     3601 tcatgatgat cgtggctccg ctgcgcacgc gctgcaacag cacgtccttc acctgccgcg
     3661 ccgaccctgc atcgaggccg gtgagcggct cgtcgagaat gatcagacgc ggctcgtgca
     3721 ccagcgcgcc cgccagcgcc accttctggc gcataccctt ggagaacccc tcgcagcgtt
     3781 catgcgcgtg cggttcgagg cccagcacgg cgatcaactc ctgcgagcgc gcctgcgcaa
     3841 tggccggttc gacctgccag aggccggcca cgaaagcgag gtattcgaac ggcgtcagct
     3901 tgtcgtagat catcggctcg tccgaaaccc aggccgtgat ccgcttggct tccacgggca
     3961 ggcgcaacgc atcgaccccg aatatggcga tgtcaccgcg gtccgggcgc agcagaccgg
     4021 caaccatccg caaggtcgtc gtctttccag ccccgttcgg accaagcagt gcgtagaact
     4081 ccccggcccg gaccgtcagg tcgagaccgt ccaccgcagg ccggtcaaag gactttgcca
     4141 gatttcggat ggaaagcgca tccgccatgc tctgctcccg aaaaagagcg gcgagcgtct
     4201 cacagcccgg ttgcggcggc gtaaacaggc atggtgaaga cgaccgcaac gcgatcaacg
     4261 agcgcgcacc cggctactcc gcggccgctg ccctgacctg cgcgagattg tccaggctat
     4321 cgatgatctg cgtcgacaac gcatcgacga tactcgcatc ggcatgtttc gagcgcatga
     4381 gcgcgatgcg gacctccggc agcggcgcga atccctcgct tggcagcagg acgcgcatgc
     4441 caggacggat cgcactttcc ggcaggacac cgacagcgag gccggacagc acggcagcac
     4501 cgacagcgga cgaattccag ctggcataca gcacgcgata gctgcggccg acggcatcga
     4561 ggcgctcggt cgccgcctga cgccagccgc aggtcggccg tccgagcgcc agcggcaacg
     4621 gtgtctcctc atgcacggag tggcgcgcgg atgtcaccca cagcagccgc tcatggcgga
     4681 cgacctcgcc agattccagc gttttcgaca gggtgacgat cgccagatcg atgtcgccgc
     4741 ccagcaaccg atcaacgagc atcggcgtcg gctcacacac caccgtcacc tcgacgcgag
     4801 ggttggaacg ggtaaatcgc gccagaattt ccggcagata gcggtcggcg taatcatccg
     4861 gtacaccaag tcggacccgg cctgacagga ccgcatccga aaaggttgcg acgcactcgc
     4921 tgctcagtcc gacaatgcga cgagcgtagc tcagcagccg gtccccatcc tcgttcagcc
     4981 gcgcgcctcg tccctccttg tggaaaatcg gacgcccaag ttgctcttcc agccgcttca
     5041 tctgcatcga aacggcggat tgcgtcttga agaccttctc ggcagcgcgc gtgaaggaac
     5101 cggtgtcggc aatggcaatg agcgaccgca actgatcgat gtccagaagg tgcatcgccg
     5161 cctcattcat tccaatggtt catgataacg atgaaaaaca ttcgttatca tcattatcga
     5221 tgatagttta taagaaaatc atggattgct gctgatggca atccattcct caatcatcac
     5281 ccgacatgga ggatccaatg tcttacaccg ttgccgatct ccggcttccg cttgtctccg
     5341 ccgcagcgga gattctgcgc acggttgtta gccggtcttc caggatcttg aaaaacctgc
     5401 gcaaccgacg cgaagttcat gcgctcgccc ggtgcgacga gcacctgctc aaggacatgg
     5461 gcctgacgcc ttcagacatc acggcggcgc tggaccggcc atggaacgtt gatccttcga
     5521 cacacctgac gagggtcgcg gccggccgaa cccgaaactc cccgaggccc tgagtgcagc
     5581 ggcgccctct ccccggcgcc actggtcagg actgaagcgc ggccctgttc tggccgcgct
     5641 tccttttcag cgcttttgcg ccttcaggat ttcatcaaag atgttctgga gtccagacat
     5701 ctgcgcttcc tgtacggtct ggccggtttc gaacatgcct ttcaggatgt cgatgccgga
     5761 ggtgatcggg tctgcctgct gttgtggctg tggccgcgcc tgtccgccgg tcaatccgcc
     5821 gagaacctgg ccaaggatgc ccccgagcag atcgcccatc gggtcctgcc cctgtcgcgg
     5881 ggccgttgcc tgtccaaggc cgcccttcag gacctgttcg ataatgccgc cgaggccatt
     5941 gttcatcgcc cccttgaaca ggccgccgag aatgatggac gcgatcatcg gcaacatctt
     6001 ctgcagcacc gtgttgccaa tgccggtcag ggcggcggcc tgctgaacca cctgcgcctg
     6061 cacctccttg ctcgcctgct gcgtaccccc aaaaatctgg gtcatgaatt ccttgccgga
     6121 attcagcatc tgcttgctgg cagcagccgt gggatcctcg aaagcagcct cgccggttcc
     6181 agacccgagc atcgacatca ggttgatgag gccatcggcg ctttgcgcct gttgctgcaa
     6241 ccccagggaa aaggccggca gcatggcgtt cacggcagct tctgcctgct cggtggagag
     6301 gccatattgt cgggcgaggg actgaaacgc cgcgcccccg tgcgcattcg cgagtatatc
     6361 ggcgagattc atcatgggta agcctctcat cctggtgccg gcccgaaggc tagttgttga
     6421 ggccggcaac ggcaatggcc attgcgccgc ttcaggatgt cttttggctc tcgatccggc
     6481 gaaccgcgcg aaacgcctca atgacgctcc aggtcccgaa cacgccccaa ccgataatca
     6541 ccaggccaat cgcaccctcg tagccccaca attgcgctcc gaagtaactg aagggcatgg
     6601 tgccgagcgt cgcacgcccc caattgaaga atgtggaata gagcggaaag ccgagattgt
     6661 tgaacgcggc attggcgaca aacagcaagc cgttgaacac ccaggcgcca gcgccaatca
     6721 ggcagaagaa gccgacatag gcagccgtct tctcagacgc gccgaacagg gccgcaatct
     6781 ggtgccggcc gagcgccagc atcgcccagg cgaacaagga atagcttgcg gcgaagacga
     6841 gcgaatcgcg cagcgtccgc cgcacgcgat catagtgacg cgcgccaagg ttctgcccga
     6901 ggatcggacc gaccgagccg ctgagtgcaa agatcacgcc gaacgccagc ggcaccagcc
     6961 ggtcaatgat tgtgctggct gccacggcct catgcccgaa ctggcgcacc acgccgatca
     7021 tgaaaccggc cgcgacgggg ctggcaatgt tggtcagcac cgcaggcgcg gcgatgtaca
     7081 ggagcggcct caggttctgc gacagatcat ccagcgtcgg ccgtgccata aggcggtgaa
     7141 ccttcaccac gcccatgaag ccgatggcgc agaacgtcag ccgcgagaac accgtggcca
     7201 gggcggcacc gtccgttcca aggcgcaggg caaagatgaa cagcggatcg agaaccgccg
     7261 tcacgatgcc ccccatcagc gtcaccatca tggcgcgacg cgcatccccc gccgcccgca
     7321 agacgccgga aaacgccatg ccgagcgcca tcagcggatt ggagggcatc gatatccaca
     7381 ggaagcgctc ggcaatggcg cgcggctgcc cggaggcacc cagggcatcg agaatccaag
     7441 gcatccccgc cagcatcacc aatgtcacgg ccagggacgc cccggttaga agcacggctg
     7501 atgagcccgc aacccttcgc gcatggtcga catcgcgcgc gccgagcgcc cgggacacga
     7561 gggccgtgcc ggcgatcatc agcccgatgt tgaaagcgat ggcgaaaaac atgaacgtgg
     7621 tcgcgtagcc gacgcctgcc gtccattcct cattccgcag caaggaaatg tagaacagcg
     7681 aaaggaagtc gacgaggaag atcgatacaa gcccgacggc gccggtcacg gtcatcacca
     7741 cgacatggcg catggtcgaa ccctgcgtca gcttcccggc cggcgctggc ctggtatctg
     7801 gtgggctggg tttggatgcg tcgttcatgc ctcgtccttc ccggtccgga ccggacgcga
     7861 ggaagggcgg ttttgacagg atcggccaaa gggtcaactg cctccatgta tcccatgccg
     7921 caatcgtgaa ccttgacggc aggacgcgcg cgcaccagaa cccagtcgag attgccgccg
     7981 ggatcatgat gaaaaagccg cccctgacga ttctcctgtg tgcgccgcgc ggcttttgcg
     8041 cgggtgtcgt gcgcgccatt gatgccgtgg aggaggcgct caggcgcttc ggccgcccgg
     8101 tttatgtgcg ccatgagatc gtgcacaatc gctatgtcgt cgaagacctc aagcgcaagg
     8161 gcgcggtctt cgtcgacgag ctgcacgaaa ttcccgacac gcaggcgcca gtcatcttct
     8221 cggcacacgg cgttcccaag tccgtgccgg cggatgcgga gcgccgcaac ctcatggcga
     8281 tcgacgccac ctgcccgctg gtcaccaaag tgcaccggga ggcggagatc caccaccggc
     8341 gcggccgcaa ggtggtgttg atcggccatg ccggacatcc ggaggttgtc ggcacgatgg
     8401 ggcagctgcc ggaaggcgcg gtgactttga tcgaaacgct ggacgacgtt ggccggcttg
     8461 ccgatcccgg tgatgcgccg ctcgcctatg tcagccagac cacgctgtcc gtcgatgata
     8521 cgcgcggcat cgtcgaggcg ctccgcgccc gctttcctgc aatccacgga ccaaacaagg
     8581 aagacatctg ttacgccacc accaacaggc aggaagccgt caagcgcgtg gcgccgcagg
     8641 tcgatgcgct gattgtcgtc ggcagcgaga actcgtcgaa ctcccagcgc ctgcgcgagg
     8701 tcgcggagcg cgccggctgt agcgtgactc ggttgctgct gcgcgccgag gatatcgact
     8761 gggcagcctt cggggccatc cggagcctcg gcatcaccgc cggtgcatcc gcgccggaag
     8821 tattggtcga ggaaatcatc gacgccttcg ctgcccgttt cgatgtgacg gtcgagaccg
     8881 tgtcgaccgc cgacgaaagc gtatttttcc cgctgccgcg caagttgcgc gatggggacg
     8941 gaccccttac gcgttcagac tgagcgcgac accgacgacc ac
//
LOCUS       DAOMXN010000090         8941 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_4133_length_8941_cov_10.204254, whole genome shotgun sequence.
ACCESSION   DAOMXN010000090 DAOMXN010000000
VERSION     DAOMXN010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 8941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8941
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4133_length_8941_cov_10.204254"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="PK812_07810"
     CDS             <1..280
                     /locus_tag="PK812_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015821669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69498.1"
                     /translation="ISSVVADGGSDARVYAFAGATALKSEPGVETVTTVETLAFARPA
                     AVSVADKRKEAQMKGYVGEACPECANFTLVRNGTCLKCDTCGSTTGCS"
     gene            555..1220
                     /locus_tag="PK812_07815"
     CDS             555..1220
                     /locus_tag="PK812_07815"
                     /inference="COORDINATES: protein motif:HMM:NF037888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69499.1"
                     /translation="MSESNRKLIFSDVIASQISTSLASNTFNILRDSQYRFEIIRLCA
                     LWDSARSDRESISTIAALVDSLEAQELVANEARSQRTSMGDGTIFGSHNEAFREYIIQ
                     SAREDAKVEGDKWAIRASLQIRRACQHVQKIQLSRRLNALRDLRDHRIAHTLADTARR
                     RSPGFRLRYRDSFSILRLSCRIIELFNLSLCGVCYKLEDVRKLHEENARLFWDGITVN
                     VLG"
     gene            1369..1674
                     /locus_tag="PK812_07820"
     CDS             1369..1674
                     /locus_tag="PK812_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015242555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system MqsR family
                     toxin"
                     /protein_id="HRJ69500.1"
                     /translation="MEKRRPTYDLEAIRLICGTEETLNMTTVAYRDAAALGFDRVDVA
                     ETIAGMDRSMFYKSMNTFGDHRVWQDVYHVPARGMVLYVKFQADIVTDFTLMSFKEK"
     gene            1677..2096
                     /locus_tag="PK812_07825"
     CDS             1677..2096
                     /locus_tag="PK812_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015242556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system MqsA family
                     antitoxin"
                     /protein_id="HRJ69501.1"
                     /translation="MAITARNLPPTMPCPETGQLLTRGVRPLTITYKGESETVDLPGY
                     YPVGEGEGVLVGEDMRASDEALARLKQKVDGIPTPETIRRVRKKLKLSQREAGALLKV
                     GENAFDKYERGLVAPSGPTAQLIRLLDRHPELADELR"
     gene            complement(2097..2822)
                     /locus_tag="PK812_07830"
     CDS             complement(2097..2822)
                     /locus_tag="PK812_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69502.1"
                     /translation="MFLQLNSELPQFCERLNAASGFEALNLLSTLALAAPSVIGWRLL
                     RGIPTRDLSHLLLVLLAAMLPLSQVVVHGFRPSWTSLADQIPQGLFALTVFVIVFWRK
                     LGLSAPMAALNILGFCFLALMVRRIMPLTPLGDLGTMAPAVLLLVITGGILMGQGRDE
                     ERQRSYAVAVQGATAAEKQRCGRLIMVAGLVLAVSLLVRMLDLPLCKAVPVGLHWLWQ
                     IGAAVVGTLLVVAVMGEKAPARV"
     gene            3292..3471
                     /locus_tag="PK812_07835"
     CDS             3292..3471
                     /locus_tag="PK812_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69503.1"
                     /translation="MAKTTKAAAKPATKAAAKPAAAKPAATKAAAKPAATKAAAKPAA
                     KAAAKPAAKPAAKKK"
     gene            complement(3577..4311)
                     /locus_tag="PK812_07840"
     CDS             complement(3577..4311)
                     /locus_tag="PK812_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69504.1"
                     /translation="MTQVLSALPLYCERAGDGPWAEPLNVLSSGAFFLAAGVGWMLWR
                     RAGRADRPIGWLALAVALIGCGSLSWHVWRQPWAFAADVVTAQVFIFAYMALALRRFF
                     RLGWPLTGAWLAVLAAATLLARQVIPSTALAGGATDIPALLALYLMGAVLVLRARVAL
                     HADMPLTGHAAAASDARHFPALRAGYALILAGILFALALAARTLDLPFCAQIPVGLHA
                     LWHILWAMAAAVLLAAAIRHGPVPAR"
     gene            4416..5207
                     /gene="xth"
                     /locus_tag="PK812_07845"
     CDS             4416..5207
                     /gene="xth"
                     /locus_tag="PK812_07845"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HRJ69505.1"
                     /translation="MKIATWNINSIRQRIGNLSSWLDRARPDIVCLQEIKCQDEQFPR
                     SEIEALGYNVAVHGQKTFNGVAILSKLPFDEVTRRLPGDESDEQARYLEAVVSVKGGV
                     LRIASIYLPNGNPLGTEKFPYKLAWMERLKRHAAGLLALEEPLVLAGDYNVIPEPRDA
                     RYPELWTGDALFQPESRARFREIAHLGFTDAVRATTDEAGLYSFWDYQAGAWQRNNGI
                     RIDHLMLSPQAADRLLAAGIDKDERAREKPSDHVPVWARLRLETP"
     gene            5204..7447
                     /locus_tag="PK812_07850"
     CDS             5204..7447
                     /locus_tag="PK812_07850"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRJ69506.1"
                     /translation="MTRLGKRLGRTLASLVALPIGLLIAFLAYLEPQAVRSLRDIVFD
                     TYQRQWPRAFNGEMPARIVDIDDDSLRRLGQWPWPRTVMARLVDAIGAADPAVIALDI
                     VFSEPDRTSPERVVALLPPSPERDALEKKLAAEDHSNDRVLAAAFERQTVISGMVLVE
                     SAEPPPVLAGFAAAGDPPHAFLPRYAGAIAPVEVLRPGLKGFGALNWVPEYDQVVRRV
                     PSVLSSAGKLAPSLGIEALRVAQGASTIIVKASNASGENAFGQQTGIVSVKVGEIVIP
                     TDFDGAVRVRYAGTRPERRIPAWKLLAGEVKKEELEGRLVFVGSSAAALSDLRATPLD
                     PVVPGVEVHAEFVEHALMGVRLSRPDWARGAETLAILLLTVVIGVAAYQLNPLLGAGL
                     ALTLIGGALVGSWRLFMSADMLLDPLLPALAGGIAFATTSVVRWKASDAERKAVRTAF
                     SRYLAPAMVERLAQEPDKLQLGGESREMSILFSDVRGFTSLSEGYKSNPQGLTQLMNR
                     LLSPLSNAIIERQGTIDKYMGDAIMAFWNAPIDIEDHAGNACLAALDMRGRLDELNRK
                     LAQEAEAAGRTHAPIDIGAGINSGACVVGNMGSDIRFDYSVLGDPVNLASRLEGQTKS
                     YGVNIILGEATARLVEGRFAIVELDRIRVKGKTEPQAIYALLGDRVMLAQPQFETLRA
                     GFAMLLENYRKQDWSEAGRDLRALQRIAYQFGVESYLDMMHDRIERFRTTPPPADWDG
                     VYTAETK"
     gene            complement(7465..8304)
                     /locus_tag="PK812_07855"
     CDS             complement(7465..8304)
                     /locus_tag="PK812_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009492280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ69507.1"
                     /translation="MSGAKHLSVVCLAILWAGPAFALDPDKQPNRALPQLEFKSVNEA
                     LRDGMKRLNDGDKPGAARVFNFAATQGNVAAQWKLGRMYADGDGVDQDDYKAFQYFSQ
                     IANTRPDEARGTMMAGVVAKAFVQLGTYHLEGIPGSPVRANPHRAFEMFHYAASFFGD
                     PDAQYNVGRIFLDGVTGPKDVRQAARWLNLAAEKGHPYAQAVLGQILFNGQGGVPRQA
                     PLGLSWILVAKQAVDPAKDAWVVELFQKVDRAATDDERKLALTFAKRHVKSGIAAAAA
                     AER"
     gene            8763..>8941
                     /locus_tag="PK812_07860"
     CDS             8763..>8941
                     /locus_tag="PK812_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRJ69508.1"
                     /translation="MVSPAAAPSRIAIVGAGPAGLIAAERLSAAGCAVTVYERMPSPG
                     RKFLLAGRGGLNLTHG"
BASE COUNT         1699 a         2869 c         2893 g         1480 t
ORIGIN      
        1 gatttcgtcc gtcgtcgcgg acggcggatc ggacgcccgc gtctacgcct tcgccggcgc
       61 gacggcgctg aagtcggaac cgggtgtgga gaccgtcacc acggtcgaaa ccctcgcctt
      121 cgcccgcccg gccgccgtct cggtggcgga caagcgcaag gaagcgcaga tgaagggata
      181 cgtcggcgaa gcctgcccgg aatgcgccaa cttcacgctc gtgcgcaacg ggacgtgttt
      241 gaagtgcgac acgtgtggaa gcacgacggg gtgttcgtga tcgcgatgca gtgacatcgt
      301 catgaccggg ctcggcccgg tcatccagga cgggattgtg atctcggacc aaacccaagg
      361 cgctggaatg cgaaaaaacg caacagaccc atgctgcaac aagagatcag caagggaagc
      421 tcgcaagatt aagaactcta gcgccgacag tgtgatctat gccatggcct gacaatcttt
      481 ctgcaccgga acgcgttagg cttgctagag agacgacgga acgagtcatt gaacacacca
      541 actatctgat tggattgagc gagagcaaca gaaaacttat cttttctgac gttattgcta
      601 gccaaatttc gacctcccta gcttctaata cattcaatat tctgagagat agtcaatatc
      661 gatttgaaat tattagatta tgtgcacttt gggatagcgc ccgctcagat agagagagca
      721 tcagcacgat cgctgcgctt gtcgattctc tagaagccca agaattagtt gccaacgagg
      781 cgcgctcgca acgtacaagc atgggggacg gcacgatatt cgggagccac aacgaggcat
      841 ttcgtgaata catcatccaa tcagcaagag aagatgctaa agtagaaggc gacaaatggg
      901 ccattcgtgc ttcattacag atacggcgcg cttgccagca cgtccaaaaa attcaacttt
      961 caaggagact taatgcactg cgagacctga gagaccacag aattgcccat actctcgctg
     1021 atactgctag gcgaagatct ccaggtttcc gtctacgcta tcgcgattcg ttttccattc
     1081 ttcgcttgtc atgtaggatt attgagttgt ttaacctaag cctctgcggt gtatgctaca
     1141 aacttgaaga cgtgaggaag ctccatgaag aaaatgcccg cttattttgg gacggcatta
     1201 cagtaaatgt tttgggataa tttcctgttc cgcgctcaat ttggaaacga acgtttgcgc
     1261 caactgaact agatatcggc gaagacggcg ttttgccccg cctgagcggc tgaagttgag
     1321 agtggatatc cttgccaagc caccataatg gtgtcataat tctccgccat ggagaagcgt
     1381 cgcccgacct atgatctcga agcgatccgg ctgatctgcg gcacggaaga gacgctgaac
     1441 atgaccaccg tggcttatcg ggatgcggcg gcgctgggct tcgaccgggt cgatgtagcc
     1501 gaaacaatcg ccgggatgga caggagcatg ttctacaagt cgatgaacac gttcggcgat
     1561 caccgggtct ggcaggacgt ctatcacgtc ccggcgcgag gcatggttct ctatgtgaaa
     1621 ttccaggccg acatcgtcac ggacttcacc ctgatgtcat tcaaggagaa atgaccatgg
     1681 cgatcacggc gcgcaatctg ccccccacca tgccctgccc tgagacgggc caactcctga
     1741 cgcgcggcgt caggccgctc accattacct acaagggcga gagcgagacc gttgatctgc
     1801 ccggctacta tcctgtcggc gagggcgaag gcgtgctggt cggagaggac atgcgcgcct
     1861 cggacgaagc gctggcccgg ctcaagcaaa aggtcgatgg cattccgacg ccggagacga
     1921 tccgccgcgt gcgcaagaag ctgaagctct ctcagcgcga ggccggggcc ttgctcaaag
     1981 tcggcgagaa cgccttcgac aaatacgagc gcgggcttgt cgcgccgagc gggccgacgg
     2041 cgcagctcat ccgcctgctc gaccggcatc ctgaactcgc cgacgagttg cgctgatcaa
     2101 acccgcgccg gtgccttctc gcccatgacc gcgacgacca gcagcgtccc gacgaccgcg
     2161 gcgccgatct gccagagcca gtgcaggccg accggaaccg ccttgcacag cggcagatcg
     2221 agcatccgca ccagcagcga cacagccagc accagccccg cgaccatgat gagccgcccg
     2281 cagcgctgct tctcggctgc ggtggcgccc tgcaccgcga cggcatagga gcgctgccgc
     2341 tcctcatcgc gcccctgccc catcaggatg ccgccggtta tgaccagcag cagcacggcc
     2401 ggcgccatcg tccccaggtc gccgagcgga gtgagcggca tgatgcgccg caccatcagc
     2461 gcgagaaagc agaagccgag gatgttgagc gccgccatgg gcgcggacag gccgagtttg
     2521 cgccagaaca cgatgacgaa caccgtcagc gcgaaaagtc cctggggaat ctggtccgcg
     2581 aggctcgtcc aggacggacg gaagccgtgc accacgacct gcgacagtgg cagcatggca
     2641 gccagcagca caagcagaag gtgcgacagg tcgcgcgtcg gaattccacg caacaaccgc
     2701 cagccgatga cggacggggc ggcaagagcc agcgtcgaca gcaggttcag cgcctcgaag
     2761 ccgcttgcgg cattgaggcg ttcgcagaac tgcggcagtt cgctgttcaa ttgcagaaac
     2821 acggcgcccc cttgcccccc ggcgcggttg tgctgtcact acaggctatc gcagcttttg
     2881 caagccgaac tcagcgccag gtcggcctct ggcgcggatg catcgatttc gatgcgacga
     2941 tggcgcgcgc cgaggggcat tgacagaggc gacggcctgc gcaagcaact gacgaataca
     3001 gtcatgacga tgtatttcat agaataatga tgcgtggatg ctgaacgcga tacttgttgc
     3061 gccccagcgg taagactaaa tttgcatttc ccgggccaaa tccttgcaat ccgaggcgaa
     3121 tcggccccgt aatccccggg acggaagcgc cgtcaggatg acccacaaag caatccgggt
     3181 tcctgtgggt gtatgcgcgg aagagtcttc ccgccgagca gcgtgcgcgt acgagcgagt
     3241 tgtacttccc cgccgcggag tcccggcatt ctccgaacca aggagcgcca catggcgaaa
     3301 accaccaagg ccgctgccaa accggcgacc aaagctgcag ccaagccggc tgctgccaag
     3361 ccggctgcca ccaaggctgc tgccaaaccg gctgccacca aggctgctgc gaagcccgcc
     3421 gccaaggctg ctgccaaacc ggccgccaag cccgccgcga agaaaaagta agatcgcacg
     3481 cttcgtgctg tcgcccgggg gtcggcaacg gttcgccgtt ttcgaccctg cgggtgtttc
     3541 cagtgccttt tcagcgggca acgggcggaa aaagcgctag cgcgccggaa ccgggccgtg
     3601 acggatcgcg gccgcgagca acacggccgc cgccatcgcc cagaggatat gccacagcgc
     3661 gtgcaacccg acagggatct gcgcgcagaa cggcagatcg agcgtgcgcg ccgccagcgc
     3721 cagcgcgaag aggattcccg ccaggatcag cgcatagccc gcccgcagcg ccgggaagtg
     3781 acgggcgtcc gaggcggcgg cggcatggcc ggtcagcggc atgtccgcat gcagcgccac
     3841 gcgggcgcgc aggaccagca cggcacccat cagataaagc gccagaaggg cgggaatgtc
     3901 ggtggcgccg ccggccagcg ccgttgacgg gatcacctgt ctggcgagca gcgtcgcggc
     3961 ggccagcacc gcaagccagg cgccagtcag cggccagccg aggcggaaga acctccgcag
     4021 cgccagcgcc atataggcaa agatgaagac ctgagccgtc acgacatccg cggcgaacgc
     4081 ccagggctgt cgccagacgt gccaggacag gctgccgcac ccgatcagcg ccaccgccag
     4141 ggcgagccag ccgatcggcc ggtccgcccg acccgcgcgt ctccacagca tccagccgac
     4201 gcctgcggcg aggaaaaatg cgccgctcga cagcacattc agcggttcgg cccaagggcc
     4261 gtcgccggcg cgttcgcaat agagcggcag ggcggagagg acttgcgtca cgggatggcg
     4321 gtctgatcga tggagggaga gcttgcttcc gggcgggcag tgtccaagat gccggactga
     4381 gccgattccg caaagcggcg ataccggacc gcagcatgaa aatcgccacc tggaacatca
     4441 actcaatccg ccagcgcatc ggcaaccttt cgagctggct ggaccgtgcg cgaccggaca
     4501 tcgtctgcct gcaggagatc aagtgccagg acgagcagtt cccgcgctcc gagatcgagg
     4561 cgctgggcta caacgtcgcc gtccacgggc agaagacctt caacggcgtc gccatcctgt
     4621 cgaagctgcc gttcgacgag gtgacccggc gcctgcccgg cgacgagagc gatgagcagg
     4681 cccgctatct cgaagccgtg gtttcggtga aggggggcgt cctgcgcatc gcctcgatct
     4741 acctgcccaa cggcaatccg ctgggaaccg aaaagtttcc ctacaagctg gcgtggatgg
     4801 agcggctgaa gcggcacgcg gcggggcttc tcgcgctgga ggagccgctg gttctcgccg
     4861 gggactacaa cgtgattccc gagccgcgcg atgcgcgcta tcccgagttg tggaccggcg
     4921 atgcgctgtt ccagccggaa tcgcgcgcgc gtttccgcga gatcgcgcat ctcggcttca
     4981 ccgacgcggt gcgcgcgacg acggacgagg ccggtctcta cagtttctgg gactatcagg
     5041 ccggggcgtg gcagcggaac aacggcatcc gcatcgatca cctgatgctg tcgccgcagg
     5101 cggcggatcg cctgctcgcg gccggaatcg acaaggacga acgcgcgcgg gaaaagcctt
     5161 cggaccatgt gccggtctgg gcgcggttga ggctcgaaac cccttgactc ggctgggaaa
     5221 gcggcttggg cgaacgctgg cgtcgctggt cgcgctgccg atcgggctgt tgatcgcctt
     5281 cctggcctat ctcgaaccgc aagcggtgcg ttcgctgcgc gatatcgtgt tcgacaccta
     5341 tcagcgccag tggccgcggg ccttcaacgg ggagatgccg gcgcggatcg tcgacatcga
     5401 tgatgactcc ctgcgccgtc tcggccagtg gccctggccg cgcacggtga tggcccgtct
     5461 ggtggatgcc atcggcgcgg ccgacccggc ggtgatcgcg ctcgacatcg tgttttccga
     5521 accggaccgc acctcgccgg agcgcgtcgt ggcgctgttg ccgccctcgc ccgagcgaga
     5581 cgcgctggaa aagaaactgg cggcggaaga ccacagcaac gaccgggttc tcgccgcagc
     5641 cttcgagcgg cagaccgtca tttccggcat ggtgctggtc gagagcgccg agccgccgcc
     5701 ggtgcttgcc gggttcgccg ccgccggcga cccaccccat gcgttcctgc cgcgttatgc
     5761 cggcgccatc gcgccggtcg aggttctgcg gccggggctg aaggggtttg gcgcgctcaa
     5821 ctgggtgccg gagtacgatc aggtggtgcg ccgcgtgccg tcggtcctgt cgagtgccgg
     5881 caagcttgcg ccctcgctcg gcatcgaggc cttgcgcgtg gcgcaggggg cttcgaccat
     5941 catcgtcaag gcttccaacg ccagcggcga aaatgcgttc ggccagcaga cggggatcgt
     6001 cagcgtcaag gtcggcgaaa ttgtcatccc gaccgatttc gacggcgcgg tgcgcgtgcg
     6061 ctacgccggc acgcggccgg agcggcgcat tcccgcctgg aagctgctgg ccggcgaggt
     6121 caagaaggag gagctggaag ggcgtctggt cttcgtcgga tcctccgcgg cggcgctgtc
     6181 ggacctgcgc gccacaccgc tcgatccggt cgttcccggg gtcgaggtgc atgccgagtt
     6241 cgtcgaacat gcgctgatgg gcgtgcggct ctcgcgtccc gactgggcgc gcggcgcgga
     6301 gactctggcg atcctgctgc tgacggtggt gatcggcgtc gccgcctacc agctcaaccc
     6361 gcttctcggg gcgggcctgg cgctgacgtt gatcggcggc gcgctggtgg gaagctggcg
     6421 cctgttcatg tcggcggaca tgctgctgga tccgctgctt ccggcgctgg ccggcggcat
     6481 cgccttcgcc accacgagcg tggtgcgctg gaaggcctcg gacgccgagc gcaaggcagt
     6541 gcgcaccgcc ttctcccgct atctggcgcc ggccatggtc gagcggctgg cgcaggagcc
     6601 ggacaagctg caactcggcg gcgaatcgcg cgagatgagc atcctgttct cggatgtgcg
     6661 cggcttcacc tcgctgtcgg aagggtacaa gtccaatccg caggggctga cccagttgat
     6721 gaaccggctg ctgtcgccgc tgtcgaatgc gatcatcgag cgccagggca ccatcgacaa
     6781 atacatgggc gacgcgatca tggcgttctg gaacgcgccc atcgacatcg aggaccacgc
     6841 cggcaatgcc tgcctggccg cgctggacat gcgtgggcgg ctcgatgaac tcaaccgcaa
     6901 gctggcgcag gaggccgagg ccgccgggcg aacccatgcg cccatcgaca tcggcgcggg
     6961 catcaattcc ggcgcctgcg tcgtcggcaa catgggttcg gacatccgct tcgactattc
     7021 cgtgctgggc gatcccgtga accttgcctc gcggctggag gggcagacca agagctatgg
     7081 tgtcaacatc atcctcggag aggcgaccgc acggctggtg gaggggcgtt tcgccattgt
     7141 cgagctcgac cgcatccgcg tgaagggcaa gaccgaaccg caggcgatct acgccctgct
     7201 aggggatcgc gtcatgctgg cgcagccgca attcgaaacg ctgcgtgccg ggttcgcgat
     7261 gctgttggag aactatcgca agcaggactg gagcgaggcc gggcgcgacc tgcgcgcctt
     7321 gcagcggatt gcctatcagt tcggcgtcga gagctatctc gacatgatgc acgaccgcat
     7381 cgaacggttc cgcaccacgc cgccgcccgc cgactgggac ggggtctaca cggcggaaac
     7441 caaataggag gggacgccgg cgtgttagcg ttcggccgcc gctgcggccg cgatgcctga
     7501 tttcacatgc cgtttggcga aggtgagcgc cagcttgcgc tcgtcatcag tcgcggcgcg
     7561 gtccactttc tggaacagtt cgacaaccca ggcgtccttg gccggatcga cggcctgctt
     7621 ggccacgaga atccaggaca ggcccagcgg cgcctgacgc ggaacgccgc cctgcccgtt
     7681 gaacagaatc tggcccagga ctgcctgcgc gtagggatgg cctttctccg ccgccagatt
     7741 cagccagcgc gccgcctgcc gcacgtcctt cgggccggtg acgccatcca ggaaaatccg
     7801 cccgacattg tactgggcat ccggatcgcc gaagaacgag gccgcgtagt ggaacatctc
     7861 gaaggcgcgg tgcggattgg cgcgcacggg cgatcctgga atgccctcaa ggtgataggt
     7921 gccgagctgc acgaaggcct tggccaccac gccggccatc atggtgccgc gcgcctcgtc
     7981 cggccgtgtg ttggcgatct gcgagaaata ctggaacgcc ttgtaatcgt cctggtcgac
     8041 gccatcgccg tcggcataca tgcggccgag cttccactgg gccgcgacat tgccctgcgt
     8101 cgccgcgaaa ttgaacacgc gcgccgcgcc cggcttgtcg ccgtcgttca ggcgcttcat
     8161 gccgtcgcgc agcgcctcgt tgaccgattt gaactcaagc tgaggcaggg cgcggttcgg
     8221 ttgcttgtcc ggatcgagcg caaaggcggg accggcccac aggatcgcga ggcagacgac
     8281 ggaaaggtgc ttggcgccag acatcagcga aaaccctgaa cccgcaatcc ggcgacactg
     8341 cggcctggat gggacaagtt caggccgacg ctggatgcga atcggcgttt gcgccgtccg
     8401 gcctgccccc tgcccgtgct cagccccatt cgtcacgacc gttcgtcccc acgcattcaa
     8461 ccgatgcatc cgtcactggc ttcctgaaaa gccttgctga gaaaaatccc tgccatggcg
     8521 gacccagaaa tcgcaatccc gcgccagcgg cagggttacc ggcgcaaaaa cggccgctgc
     8581 gggcctgttt cacaccgctc cgtgccaaaa ccgcagcaag cgacggctct ttcgttcacg
     8641 tcggtttgtg gcgggatcgg ggcgcatttc ggccggttcc ggcgcgctcc atcaattccc
     8701 tcggctgttg ccgggatgtc acaaggcttg acccgccgtc ggccgcatga gaacgcccag
     8761 gcatggtgtc ccctgccgcc gccccttccc gcatcgccat cgtcggcgca ggtcctgcag
     8821 ggctgatcgc tgccgagcgc ctgtcggctg cgggctgcgc ggtcacggtc tacgagcgca
     8881 tgccctcgcc ggggcgcaag ttcctgctgg ccgggcgcgg cggcctcaat ctgacccatg
     8941 g
//
LOCUS       DAOMXN010000091         8936 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_4137_length_8936_cov_11.177345, whole genome shotgun sequence.
ACCESSION   DAOMXN010000091 DAOMXN010000000
VERSION     DAOMXN010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 8936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8936
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4137_length_8936_cov_11.177345"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1273
                     /locus_tag="PK812_07865"
     CDS             <1..1273
                     /locus_tag="PK812_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HRJ69509.1"
                     /translation="PGPASGAGDAEKARELRRIKALASASLVAAVALLVVAKLLEPRW
                     PTLAYVAAFAEAAAIGGLADWYAVVALFRHPLGLPIPHTAIVPKNQQRIAEKLGEFIQ
                     NHFLTSQAVETKLKQVDFAGFIADWLVDKGRSARLSIFGLRLLPDALSAAENSGLKSY
                     VTQRVMAQVESIDIGPLAAGALRAFLNRERRQTILDELLDALLSTLENDEALAALREK
                     IRAELPTLLNLYRADAFLLRRIVGTAGRFLEEVQADPEHPFRAEFDRMLNSLLDRLES
                     EPGFARKLQSLKHDFLARPELAALGQMLWANAREFVDRSAAGQNDLLRRHLTNAFIQV
                     GEQLRADPEMRAEINGGAVMVLHRFIEDHKGGVSGFISDQVKSWNLGQLVNLIELNIG
                     RDLQYIRFNGMVIGGLAGLLLYVLKQALGLH"
     gene            1367..2992
                     /locus_tag="PK812_07870"
     CDS             1367..2992
                     /locus_tag="PK812_07870"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HRJ69510.1"
                     /translation="MSRLSGVDASFLYLESADMPMHVAGLTLYEMPPGFKGSFYEHFK
                     KFFAGRVHLIPIFNKKLAHAPLNLDHPGWVDEDNLDLDYHIQGVRLPAPGTRAQLDAL
                     IAQMHAELMDRTRPLWRFTVIEGLEDGKAALYSKVHHAAVDGSSGMIITRALYDLTPE
                     PRVVEPPAPKAPSQPAPAKDLASSLTDLMAGMIRQQQAALRAMPEVLGAVVNAVAPPS
                     DKNTPITELFPNIKLPKLHLIAPKTPFNTTVGKSRAYASRSISLADAKKVAKVTDTKI
                     NDVVMAVSGGALRKYLAARKALPKSTLLAFVPISTREAGNTEINNQVSGMICPIGTNE
                     KDPLKRLVEIRSASSEAKEVASSVKSAVPQDFTFLGAPTIINLMASLGARFRIADWMV
                     PVGNVTISNNAGPPVSLYCAGARVAALYPVSIPGHGIALNITVQSYCDQLDFGITADA
                     KAAPDLEMLADALVESMEELKAAAAAKAAASAPAAEAKPAAQGKTEPPPAVKAAPKPV
                     VSPAVAKVAKAVKAAKSAKAAAGKPAARRGKAE"
     gene            3141..3911
                     /locus_tag="PK812_07875"
     CDS             3141..3911
                     /locus_tag="PK812_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRJ69511.1"
                     /translation="MTAVLHHMDPPSKALLFMEGRAFFELGAFFWTLPLLNRAPQGDG
                     HPVLVLPGMLTSDASTKALREFLKGRGYDVHGWRQGRNVGLTAALQSAMVDQIKTLAD
                     AHGRKVSVVGWSLGGLYARQLGKVLPDHVRSVTTLGSPFAGSPRATNAWRVYEFVSGQ
                     KADYRDYHFGGDLAETPPMPTTAIYSRTDGVCAWQVCKEQPGALAESIEVPGSHCGMG
                     HNPAVLYALADRLAQEEGNWKPFERTGWRSLVFPDPHR"
     gene            complement(3981..4232)
                     /locus_tag="PK812_07880"
     CDS             complement(3981..4232)
                     /locus_tag="PK812_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6489 family protein"
                     /protein_id="HRJ69512.1"
                     /translation="MKVTIDIDCTPQEARAFIGLPDVEPMQKAVMGEIERRMMSEIEN
                     FGPEHMMKTWVPMLMQGASWLPEFFQQAMKTATGGSAKK"
     gene            4371..4691
                     /locus_tag="PK812_07885"
     CDS             4371..4691
                     /locus_tag="PK812_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HRJ69513.1"
                     /translation="MIVATTNELAGYRIVQHLGMVRGITVRSRSVVGNFGGAIQAFFG
                     GKLSVYVTLAETAREEAFRQMVDHAEQLGANAVVAMRYDANEIMAGITEVLAYGTAVR
                     VEMA"
     gene            4929..5005
                     /locus_tag="PK812_07890"
     tRNA            4929..5005
                     /locus_tag="PK812_07890"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4963..4965,aa:Trp,seq:cca)
     gene            5147..6517
                     /locus_tag="PK812_07895"
     CDS             5147..6517
                     /locus_tag="PK812_07895"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HRJ69514.1"
                     /translation="MNPGIAADPVFQAPFITPPAERLGFLEAIRAIMRNPIEVWPQEF
                     YRTGYMRGRFGRRTVVHVARPDLIHAVFVEEARSLHRSDIAQAILKPAMREGLLTAED
                     EVWRAQRRIAAPAFRQQMLDSLVPLMDKAGDALAERLKARRGQAVNVMPEMIRATFSI
                     ISDTMFGETREEDARLIETEVGGYLSSVGKVDLFDMFGVPYWVPRPWKRAGKLAVKRL
                     RARAERSIADLRAKGATGGSLTALLMNASDPETGGAMSEDQLIDNIITFIGAGHETTS
                     LALTWSLYLVAQQPNLQKRLADEVHAVAGQGPILAEHVARLELHERVLKEAMRLYPPV
                     ALIGRKVVKPMTLNGDALRPGDQVVCGVLPLHRSPTLWENPDAFDPDRFLPERSAGRP
                     RYAWMPFGAGPRICIGMGFAMMEGIVLLAHMMRAVRFAPVDGHEVKPVVRVTMRPRGG
                     MPLKVA"
     gene            6653..6850
                     /gene="secE"
                     /locus_tag="PK812_07900"
     CDS             6653..6850
                     /gene="secE"
                     /locus_tag="PK812_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HRJ69515.1"
                     /translation="MAKPNPFEFIQEVRSEAKKVTWPTRKETGITTAMVLVMVFFASI
                     FFLLADMAIRFVVGLVLGYGS"
     gene            6881..7414
                     /gene="nusG"
                     /locus_tag="PK812_07905"
     CDS             6881..7414
                     /gene="nusG"
                     /locus_tag="PK812_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HRJ69516.1"
                     /translation="MAKRWYIVHAFSNFEKKVADAIREGAEQRGLSDLFEEILVPTEK
                     VTEVRRGRKIETDRKFFPGYVLVKVDLTDDAYHLIKNTPKVTGFLGADKKPIPIPDHE
                     AERIKGQVVDSTAKPKSAIMFSVGENVRVSDGPFASFSGVVEDVDAVRSRLKVAVSIF
                     GRATPVELEFGQVEKVK"
     gene            complement(7508..>8936)
                     /locus_tag="PK812_07910"
     CDS             complement(7508..>8936)
                     /locus_tag="PK812_07910"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HRJ69517.1"
                     /translation="HNIEAEQALLGAILVHNEAYDRVADFLRPEHFYEEIHARIFETA
                     GRLIHAGKIATPITLKTFLPDTQDLGGMTVAQYLVRLAQSATTIINAKEYGRAIHDLA
                     LRRQLITIGEDMVNTAFDAPVEASPRAQIEDTERKLFSLAEAGRYEGGFQSFDLALTT
                     AMDVAARAYERDGGLSGISTGLSDLDRQMGGLQRSDLVILAGRPGMGKTALATNIAYN
                     IARAWSGEKLADGTRVTHNGGIVGFFSLEMSAEQLATRIIAEQSGIPSYKIRRGDIDE
                     RDFESLTDAVRVMQEIPLYIDQTGGISIAQLTARARRLKRQQGLDLLVVDYIQLLSGA
                     AKKGENRVQELTEITTGLKALAKELDVPILALSQLSRQVESRDDKRPQLSDLRESGSI
                     EQDADVVMFVFREEYYLKNKEPKPGTEDHLKWQQDMEKVHGRAEVIIGKQRHGPTGTI
                     PLHFDANVTRFSNLAVEGSLPERMD"
BASE COUNT         1527 a         2795 c         2939 g         1675 t
ORIGIN      
        1 gcccggtccg gcgtccggcg ccggcgatgc cgagaaggcg cgggagctgc ggcgcatcaa
       61 ggcgctcgcc tcggcgagcc ttgtcgcggc ggtggcgctg cttgtcgtgg ccaaactcct
      121 ggaaccgcgc tggccaacac tcgcctacgt cgcagccttc gctgaagcgg cggcgatcgg
      181 cggccttgcc gactggtacg ccgtggtcgc gctgttccgc catcccctcg gcctgccgat
      241 tccccacact gccatcgtgc ccaagaacca gcaacggatc gcggaaaagc ttggcgagtt
      301 catccagaac catttcctga cgtcgcaggc ggtcgagacc aagctgaagc aagtcgactt
      361 tgccggcttc atagcggact ggctggtcga caaggggcgc agcgcccggt tgtcgatctt
      421 tgggttgcgg ctactgccgg atgcgctgtc ggcggccgaa aattcagggt tgaagagcta
      481 cgtgacgcag cgcgtcatgg cgcaggtcga atcgatcgac atcggaccac tcgcggcggg
      541 cgcgttgcgc gcgttcctca accgagagcg ccggcagacg attctcgacg aactgctcga
      601 cgcgctgctg tcgactctgg agaacgacga agcgttggcg gcgctgcgcg agaaaattcg
      661 ggcggagctg ccgacgctgc tcaatctcta ccgcgccgat gctttcctgt tgcgccgcat
      721 cgtcggcacc gccggccgct tcctggaaga agtgcaggct gatcccgagc acccgttccg
      781 tgcggagttt gatcgcatgc tgaacagcct gctcgacagg ctggaatccg agcccggctt
      841 tgcgcggaag ctgcagtcgc tgaagcatga cttcctcgcg cgaccggagc tggcggcgct
      901 cggccagatg ttgtgggcga acgcccggga attcgtcgac cgcagcgcag ccggccagaa
      961 cgacctgctg aggcgccacc tgaccaacgc ctttattcag gtcggggaac aactgcgcgc
     1021 cgatccggag atgcgggcgg agatcaacgg cggggcggtc atggtgctgc accgtttcat
     1081 cgaggaccat aaaggcggcg tttcaggctt tatctcggat caggtgaagt cgtggaacct
     1141 tggccagttg gtcaacctga tcgaactcaa tatcggccgg gacctgcagt acatccgttt
     1201 caacggcatg gtgatcggcg gcctggcagg gctgctgctc tatgtgctga agcaggcgct
     1261 gggtttgcat tgacccttat ttgacccgcc gcgcacgcgg gtgacatagt gcgtgcaaag
     1321 acggttcgcc ggatacaagg cggccgcatc gtgaggaggg accagcatgt caaggctcag
     1381 cggcgtcgat gcgagctttc tctatctcga atccgctgac atgccgatgc atgtcgcggg
     1441 cctgacgctt tatgagatgc cgcccggctt caaggggtcg ttctacgagc acttcaagaa
     1501 gttcttcgcg ggccgggtgc acctgatccc gatcttcaac aagaagctgg cgcatgcgcc
     1561 gctcaatctg gatcaccccg gctgggtgga cgaggacaac ctcgatctcg attaccacat
     1621 ccagggcgtc cggctcccgg cgccgggcac ccgggcgcaa ctcgatgcgc tcatcgcgca
     1681 gatgcacgcc gaactgatgg accgcacgcg cccgctgtgg cgcttcacgg tgatcgaggg
     1741 actggaggac ggcaaggccg cgctttattc gaaggtgcat cacgccgctg tcgacggcag
     1801 ttccggcatg atcatcacgc gggcgctcta cgatctgacg ccggagccgc gcgtggtcga
     1861 gccgcccgcg ccgaaggcgc cgtcacagcc ggccccggcg aaagaccttg cgtcgtcgct
     1921 gaccgacctc atggccggca tgatccggca gcagcaggca gcgcttcgcg ccatgccgga
     1981 ggtgctgggc gctgtggtca acgccgttgc cccgccatcc gacaagaaca cgccgatcac
     2041 cgagctgttt ccgaacatca agctgccgaa actgcatttg atcgcgccga agacgccgtt
     2101 caatacaacc gtcggcaagt cgcgtgccta cgcttcgcgc agcatttcgc tggcggatgc
     2161 aaagaaggtc gcgaaggtga ccgataccaa gatcaacgac gtggtgatgg cggtcagcgg
     2221 cggtgcgctg cgcaagtacc ttgccgcgcg caaggcgctg ccgaagtcga ccctgctcgc
     2281 cttcgtgccg atctcgacgc gcgaggccgg caataccgag atcaacaacc aggtttcggg
     2341 catgatctgt ccgatcggca cgaacgaaaa ggacccgctg aagcggctgg tcgagatccg
     2401 ctccgcctcg tccgaggcca aggaggtcgc cagttcggtg aagagcgcgg tgccgcagga
     2461 cttcaccttc ctcggcgcgc cgacgatcat caatctgatg gcttcgctcg gagcgcggtt
     2521 caggatcgcg gactggatgg tgccggtcgg taacgtgacg atctccaaca acgccgggcc
     2581 gccggtttcc ctgtattgcg ccggtgcgcg cgtcgcggcg ctgtatccgg tttccatccc
     2641 cggccacgga atcgcgctga acatcacggt ccagagctat tgcgaccagt tggacttcgg
     2701 catcacggcc gacgccaaag ccgctccgga cctggagatg ctggcggatg cgctggtcga
     2761 atcgatggag gaactgaagg ccgctgccgc cgccaaggcc gccgcatcgg cgccagcggc
     2821 cgaagccaag ccggcggcgc agggcaagac ggagccgcct cctgcggtca aggccgcccc
     2881 gaagcccgtg gtttcgcctg ccgttgccaa agtcgccaag gccgtgaagg cagcaaagtc
     2941 ggcaaaggcc gcagccggga agccggcggc gcgccggggc aaggcggagt gaagcaaata
     3001 gcgaaatccc ggcaaatgca gtgtcgattg cagggatttc ctaacttgac cggaggagaa
     3061 ttccggttga aagttcacat gtgaattgtg tcgcctgcgc gtattcgagt gcatccgatt
     3121 ttgactggga ggggacccga atgaccgccg tactgcatca catggacccg ccatcgaagg
     3181 cgctgctgtt catggaaggg cgcgcctttt tcgagcttgg cgcgtttttc tggaccctgc
     3241 cgctgctgaa cagggcgcca cagggcgatg gacatcctgt tctcgttctt cccggcatgc
     3301 tgactagcga tgcgtccacg aaggcgctgc gcgagttcct gaaagggcgg ggctacgatg
     3361 ttcacggctg gcgacaaggg cgcaatgtcg gccttaccgc cgcgcttcaa tccgccatgg
     3421 tggatcagat caagacgctg gcggatgcgc atggtcgcaa ggtctccgtt gtcggctgga
     3481 gcctcggtgg cctctatgcg cgccagctcg gcaaggtcct gccggaccat gtccgctcgg
     3541 tgacgacact cggcagcccc ttcgccggca gcccgcgggc gacaaacgcc tggcgcgtct
     3601 acgaattcgt cagtggccag aaggcggact accgcgacta tcatttcggc ggcgatctgg
     3661 cggaaacgcc gcccatgccg acgacggcga tctacagccg cacagatggc gtttgcgcct
     3721 ggcaggtctg caaggagcag ccgggagctc tcgcggaaag catcgaggtt cccggcagcc
     3781 actgcggcat gggtcacaat ccggccgttc tctatgcact ggccgaccgt ctcgcgcagg
     3841 aggagggcaa ctggaagccg ttcgagcgca ccggctggcg ttccctcgtc tttcccgatc
     3901 cccaccgctg atcagtcacg aggctgattg gacacggcaa cggcgcaccg gcggcgcgcc
     3961 tgacgcacgg gcggcgcgcg ttattttttc gcgctgccgc cggtcgcggt tttcatggcc
     4021 tgctggaaga actccggcag ccagctggct ccctgcatca gcatgggaac ccaggtcttc
     4081 atcatgtgct cgggtccgaa gttctcgatc tcggacatca tgcggcgctc gatttcgccc
     4141 atgacggcct tctgcatcgg ctcgacatcc ggcagcccga tgaatgcgcg cgcctcctgc
     4201 ggggtgcaat cgatgtcgat cgtgaccttc atggtggtcg cttcctctcg gggctgctga
     4261 tttgggtgaa ggttcgcacg cgcgcgcgga cccgtccaga ggcgtcgata caccaaatcg
     4321 caaccccttg tgctcagcat gctggccatc atcgcttcag gagcatcgca atgatcgttg
     4381 ccacaaccaa tgagcttgcc ggttatcgca tcgtgcagca ccttgggatg gtccgaggga
     4441 tcaccgtgcg ctcgcgctcg gtcgtcggca atttcggcgg agcgatccag gcgttcttcg
     4501 gcggtaagct ttcggtctat gtgacgctgg cggaaaccgc gcgcgaagag gccttccgcc
     4561 agatggtcga tcatgccgag caactcggcg ccaatgccgt cgtcgcgatg cgctatgacg
     4621 ccaatgagat catggccggc atcaccgagg ttctggccta cggcacggct gtccgtgtcg
     4681 agatggcgtg actatcgctt tttcttcttc aaccggacga agggcaggaa gatccggcgc
     4741 aggccgggca ggcgaatgcg ggtcatggca ggctcctgtg cgatggcgtt tcactggcgc
     4801 caggactacg ccagcggcgc cccttgttcc gcgcggcagc gcacgtgcca ggtcgccaac
     4861 aacgctctgg ccttccggca ccaacggtgc taatgcggcg gcctgcgccg ggtctgatag
     4921 acccctgcag gagtgtagct caattggtca gagcaccggt ctccaaaacc gggggttggg
     4981 ggttcgagtc cctccactcc tgccatctcg gcattgaatt tcctgacgaa tttgtaatcg
     5041 ttacgtcgtt accctgccgc gtcttcgcca cggttttgcc ctcggtcggg ggccccatgc
     5101 gtctgggcgc gcaacgagaa ggggggcctt ggccccggac tgttccatga accctggcat
     5161 cgccgccgat cccgtcttcc aggcaccgtt cataacgccg ccagccgaac ggcttggctt
     5221 ccttgaggcc attcgcgcca tcatgcgcaa tcccatcgag gtctggccgc aggagttcta
     5281 ccgcaccggc tacatgcgtg gccgtttcgg gcggcgcacc gtggtgcatg tggcgcgacc
     5341 ggacctgatc cacgcggtgt ttgtcgagga ggcgaggtca ctgcatcgct cggatatcgc
     5401 gcaggcgatc ctgaagccgg cgatgcggga gggcctgctc acagcagagg atgaagtctg
     5461 gcgcgcccag cggcgcatcg ccgcgcccgc ctttcgccaa cagatgctcg attccctcgt
     5521 gccgttgatg gacaaggccg gcgacgcgct ggccgagcgg ttaaaggcgc ggcgtggtca
     5581 ggcagtcaac gtgatgccgg agatgatccg ggcgaccttc tcgatcatct ccgacacgat
     5641 gttcggcgaa acgcgcgaag aggatgcacg gctgattgaa accgaggtgg gcggatacct
     5701 ttcctcggtc ggcaaggttg acctgttcga catgttcggc gtgccgtact gggtcccgcg
     5761 cccctggaag cgggcaggaa agctggcggt gaagcgattg cgcgcccgcg ccgagcggtc
     5821 gattgccgac ctgcgggcga aaggcgcgac gggtggcagc ctgacggcct tgctgatgaa
     5881 cgcctcggat cccgaaaccg gcggcgccat gagcgaggac cagcttatcg acaacatcat
     5941 cacgttcatc ggggcagggc acgagacgac atccctggcg ctgacatggt cgctgtatct
     6001 ggtcgcccag cagcccaatc tccagaagcg tctggcggac gaggttcacg ccgttgccgg
     6061 tcaagggcct atcctggcgg agcatgtcgc gcgcctggaa ctgcacgagc gcgtgctgaa
     6121 ggaagccatg aggctctatc cgccggtcgc cctcatcgga cgcaaggtcg tcaagccgat
     6181 gacgctcaat ggcgatgcct tgcggccggg agaccaggtc gtatgtggcg tgctgcccct
     6241 gcatcggtcc ccgacgctct gggagaaccc ggacgcgttc gatcccgacc gcttcctgcc
     6301 ggaacgctcg gctggcaggc cgcgctatgc ctggatgccg ttcggcgccg gtccgcgcat
     6361 ctgcatcggc atggggtttg ccatgatgga aggtatcgtc ctgctggcgc atatgatgcg
     6421 cgcggtccgc ttcgcgccgg tcgacgggca tgaggtcaag ccggtggtgc gcgtgaccat
     6481 gcgacccagg ggcggcatgc cgctgaaggt cgcgtgatcc ggctgcagct tgcaatgcag
     6541 ggcgagagcg cgtatatgcc cctcattccc tgacattgcg acgtgcatcc cgaaccgccc
     6601 ggcggaacga ggtgtcttgt cggaaggggc ggtccagata cggcggttcg agatggccaa
     6661 gccaaatccg ttcgaattca tccaggaagt gcgctccgaa gcgaagaagg tgacctggcc
     6721 gacgcgcaag gaaaccggaa tcaccacggc catggtgctt gtgatggtgt tcttcgcctc
     6781 catcttcttc cttctggccg acatggccat ccggttcgtc gtgggtcttg tcctcggcta
     6841 cggcagctga tttcttcctt tctgcggcag gcataaaccc atggccaagc gctggtatat
     6901 cgttcacgca ttctccaact tcgaaaagaa ggtggcggat gccatccgcg agggcgccga
     6961 acagcgcggc ctttccgatt tgttcgagga aatcctcgtg ccgaccgaga aggtcacgga
     7021 agtgcgtcgc ggccgcaaga tcgagacgga tcgcaagttc tttcccggct atgtgctggt
     7081 gaaggtcgac ctgacggacg acgcctatca cctgatcaag aatacgccga aggtcacggg
     7141 cttcctcggc gccgacaaga agccgatccc gatcccggac cacgaggccg agcgaatcaa
     7201 ggggcaggtg gtcgattcca ccgcgaagcc gaagtcggcg atcatgttct ccgtcggcga
     7261 gaacgtgcgt gtctccgacg ggccgttcgc ctccttctcc ggcgttgtcg aggatgtgga
     7321 tgcggtgcgc tcccgcctca aggtggcggt gtcgatcttc ggtcgcgcca cgccggttga
     7381 gctggagttc ggtcaggtcg agaaggtgaa gtaggcgcgc cagtccgcgc gcttggtgaa
     7441 ggccggccgt cgcgccggcc tttttttgct ttccagcctg atggttcggg ggccggcggc
     7501 gctgcgccta gtccatccgc tccggcaggc tgccctcgac cgcgaggttg gaaaagcgcg
     7561 tgacgttggc gtcgaagtgc aggggaatcg tgccggtggg gccgtggcgc tgcttgccga
     7621 tgatgacttc cgcccttccg tgcaccttct ccatgtcctg ctgccatttc agatggtctt
     7681 cggtgccggg cttcggctcc ttgttcttca ggtagtattc ctcgcggaac acgaacatta
     7741 ccacgtccgc gtcctgctcg atggagccgg attcgcgcag gtccgacagc tgcggccgct
     7801 tgtcgtcgcg cgattccacc tgacgcgaga gctgcgacag cgcgaggatc ggcacgtcga
     7861 gttccttggc cagcgccttc aggccggtgg tgatctcggt gagttcctgc acgcggttct
     7921 cgcccttctt cgctgcgccg gagagaagct gaatgtagtc cacaaccagc agatcgaggc
     7981 cctgctggcg cttgagacgg cgggcacgcg cggtcaattg cgcgatcgag atgccgccgg
     8041 tctggtcgat atagagcggg atttcctgca tcacgcgcac ggcatcggtc agcgattcga
     8101 agtcccgctc gtcgatgtcg ccgcgccgga tcttgtagga ggggatgccg gactgctcgg
     8161 cgatgatgcg ggtggcgagc tgctcggccg acatttccag cgagaaaaat ccgacgatgc
     8221 cgccgttgtg ggtcacgcgc gtgccatccg ccagtttctc gccggaccag gcccgggcga
     8281 tgttgtaggc gatgttggtg gcgagcgccg tcttacccat gccggggcgt ccggccagga
     8341 tgacgaggtc ggacctctgc aatccgccca tctgccggtc gagatcggac aggccggtgg
     8401 aaatgccgga tagcccgcca tcgcgctcgt aggcgcgggc agcgacatcc atcgccgtgg
     8461 tcagagcaag atcgaagctc tggaatccgc cctcgtagcg gcctgcttcc gccagtgaga
     8521 acagtttgcg ctcggtatcc tcgatctgcg cacgagggct ggcctctacc ggcgcgtcga
     8581 aggcagtgtt gaccatgtcc tcgccgatag tgatcaactg gcggcgcagg gcgagatcgt
     8641 gaatcgcgcg gccatattcc ttggcgttga tgatggtggt cgcgctttgg gcgaggcgta
     8701 cgaggtattg cgccacggtc atgccgccga ggtcctgcgt atcgggcagg aaggtcttca
     8761 gcgtgatcgg cgttgcgatc ttgccggcat ggatcagccg cccggcggtc tcgaagatgc
     8821 gggcgtggat ctcctcgtag aagtgctctg gacgcaggaa gtccgccacc ctgtcatagg
     8881 cctcgttgtg tacgagaatc gcgcccagta gcgcctgctc cgcctcgatg ttgtgc
//
LOCUS       DAOMXN010000092         8931 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_4142_length_8931_cov_11.794953, whole genome shotgun sequence.
ACCESSION   DAOMXN010000092 DAOMXN010000000
VERSION     DAOMXN010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 8931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8931
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4142_length_8931_cov_11.794953"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1256)
                     /locus_tag="PK812_07915"
     CDS             complement(<1..1256)
                     /locus_tag="PK812_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRJ69518.1"
                     /translation="MPSLTAAAVGAPAANIQRVVSPKGIEAWLVEDYTVPIVSMDFAF
                     IGGAALDPSDRNGLSNLMSGLYDEGAGDFDARAYQERLEEYAVEISFSSSKDKLEGSL
                     RTLVENLQTAVDLLALAVQAPRFDEDAVQRVKAQIIAGLKRDENDPDYKVWRAFQQAA
                     YPGHPYGRPTLGTIESVTALTRDDILASHQARLTREGLRIGVVGAIGPQALSKALDTI
                     FGALPATAAQPAIADTAMAGLGTNRIVPVAVPQSTLALGRPAPGRKDPDFLAVFVVNH
                     VLGGGAFTSRLWNEVREKRGLAYSVWSQLSTGRHANAFLAGTTTSNERAAESLAIIRQ
                     EVLRMAESGPTADELDRAKKFLIGSYALRFDTSRKIAGNLVELQVEDMGMDYIATRNE
                     RLAALSLDDSRRAAGRLLGDGALLVAA"
     gene            complement(1287..2564)
                     /locus_tag="PK812_07920"
     CDS             complement(1287..2564)
                     /locus_tag="PK812_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004448175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRJ69519.1"
                     /translation="MTGQKTAALSHFTLANGMDVLVIPDHRAPVVTHMVYYRNGAADD
                     PHGKSGIAHFLEHLMFKGTAKYPKGVFSDIVSELGGQENAFTGHDYTAYFQRVAKEHL
                     AKVMELEADRMNGLVLDEAEVASERDVILEERKMHVDADPAAQLYESLSAALYTHHPY
                     GTPIIGWEHEIEGLGRDDALAYYKRFYTPENAILIVAGDVEGDTVRTLAEASYGAVKR
                     RNEPPQRKRPQEPPPRAERLVTLADEKVEQPTVYRLYLAPSHATAEGSDAYALEVLSQ
                     ILAGSSAGIAYRQLVVEKQKAVTVSSWYRNDALDQGTFGLYAVPADGVSLAELDGEID
                     AIIAGIAEHGVEEAVLRRARTRLVADMIYAQDSQAQLARMYGAALCTGGSVEDVRGWP
                     AKIAEVTIANIKHVAGRYLQRRLAAVGHLKKAA"
     gene            complement(2689..3333)
                     /locus_tag="PK812_07925"
     CDS             complement(2689..3333)
                     /locus_tag="PK812_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69520.1"
                     /translation="MVVSQCVRAAALAALVVAGGPAFAQMDTLTSVFRVIGIAPDDKE
                     PIEYRERPPLVVPPSVGRLRAPEEPVAERNANWPQDPDVLARKRKAEDAKQPVAKDRS
                     YDPSYGAQVPLNEAQRRNRYAGVPTTYQDTINRNQNDRNNLYLSPAEVGRVQAAARAQ
                     QPTLRPGEEPTRQYLTEPPKGYRRAASGAPVKATIDPIQNSDAVELDVLKSQRR"
     gene            complement(3385..3876)
                     /gene="lspA"
                     /locus_tag="PK812_07930"
     CDS             complement(3385..3876)
                     /gene="lspA"
                     /locus_tag="PK812_07930"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017485654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HRJ69521.1"
                     /translation="MSRKPFRLGLIAALATLALDQASKLFLLFVYGLPSAYPVRLGPY
                     FDLTVVWNRGISYGLFQQDTALGRWLLTAFMIAAATFLAVWMYRARSRVTALGLGLIV
                     GGAIGNAIDRAAYGAVFDFVHFHVGSFSWYVFNLADAAIVLGVPLLLYPEPKDGRNAA
                     KTP"
     gene            complement(3878..6832)
                     /gene="ileS"
                     /locus_tag="PK812_07935"
     CDS             complement(3878..6832)
                     /gene="ileS"
                     /locus_tag="PK812_07935"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HRJ69522.1"
                     /translation="MTDKTPDSVDYSKTLFLPETPFPMRAGLPQKEPEILARWQRNDL
                     YGQLRRQSKGRDKFVLHDGPPYANGNIHIGHALNKILKDMVTKSQQMLGFDSNYVPGW
                     DCHGLPIEWKIEEEYRARGKNKDEVPVVEFRRQCRDFATHWLNVQREEFKRLGVIGDW
                     DNPYSTMAFHAEAQIAREIMKFARTGQLYRGSKPVMWSVVEKTALAEAEVEYHDHTSD
                     MVWVAFPVKTGLDASVVIWTTTPWTLPGNRAISFSNRIDYGLYEVTANERPFGPVVGA
                     RYILATKLADDVAKNAKITLALREAVPAGMLAGMTCAHPLAGHIPGYEFDVPLLDGDH
                     VTDDAGTGFVHTAPGHGREDFDIWMASGKLLSARGIDTHIPYTVDADGAFTREAPGFE
                     GHRVLTDTGEKGTANEAVIKALIEAGRLVARGRLKHQYPHSWRSKKPVIFRNTPQWFI
                     AMDRKIEALGDKSLRDVSLAAIRATQWVPAAGENRINGMIENRPDWVVSRQRAWGVPI
                     TVFVHRESGEMLADARVDEAIAKAFDQEGGDAWFADVDGSRFLKPFGHDPAAYEKVTD
                     VLDVWFDSGSTHTYTLEDPVHFPGLAGIRRKVDGGRDTVMYLEGSDQHRGWFHSSLLE
                     SCGTRGRAPFDIVLTHGFCLDEKGQKMSKSLGNVTAPQKVMDESGADILRLWVAASDY
                     SDDLRIGPAIMKNFVETYRKMRNTLRWMLGSLAHFNASDSIPERAMPELERLMLHRLA
                     VLDGEVRDAYAAFDYKRVVSVLAQFMNTELSAFYFDIRKDTLYCDAPSSRTRRAALTV
                     IDRLFDALSKWLAPILSFTAEEAWIARYPEREADASLSVHLEVFPEIPAFWRDDALAE
                     KWEQVKAVRRVITGALEVERAAKRIGSSLEAAPVLHVEMPALNTALEGVNIAEVAIVS
                     DVLRGLREPAAGAFRLPDVPGVAVEVHPAQGKKCSRSWKYFDATTADPDFPDVTPRDA
                     AALKERAALGLG"
     gene            complement(7015..7995)
                     /locus_tag="PK812_07940"
     CDS             complement(7015..7995)
                     /locus_tag="PK812_07940"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HRJ69523.1"
                     /translation="MTRTIPPDVSGPAPGSPVLLRDPSGLPETLAGPVVAIGNFDGLH
                     RGHKHVIGRAGAMAARLGRPLAILTFDPHPRAYFQPDVARFRLSAPRTQAMLAATLGV
                     DALIVLTFDAALAGLSAEAFVREVLVGRLGVAGVVAGADFHYGSRRSGSPETLRAAGA
                     RFGFAVEIVDQVAEGAMAVSSTRIREALAAGDIAAANAMLGHEWFVSGPVIHGQKLGR
                     TLGFPTANLALDAGCGLRHGIYAVRVAHAGNTYGGVASFGRRPTVDNGPPLLEVVLFD
                     FSGDLYGQMLTVAFLAFLRPEEKFDSLDALVAQMNRDAEKARAIVRQTKI"
     gene            8134..8517
                     /locus_tag="PK812_07945"
     CDS             8134..8517
                     /locus_tag="PK812_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRJ69524.1"
                     /translation="MAINTSMPVLVVDDYQTMVRIIRNLLKQIGFEDVDDASDGVSAL
                     ERLRQRRYGLIISDWNMQPMSGYELLRHVRSDIDMRALPFIMVTAESKTDNVIAAKRA
                     GVSNYIVKPFNAQTLKSKIEAVCCG"
     gene            complement(8523..>8931)
                     /locus_tag="PK812_07950"
     CDS             complement(8523..>8931)
                     /locus_tag="PK812_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HRJ69525.1"
                     /translation="ERNQELICANPDIVVERGHKLVPCAGAIADLYERMGGRVTFVGK
                     PHPLVYDFALREMERVLGRTVTRDRILGIGDAIRTDVAGAAAFGIESLFVIDGIHAQE
                     TGLKDGGLSADSLAAFFARQALKPTFATRALHP"
BASE COUNT         1577 a         2876 c         2952 g         1526 t
ORIGIN      
        1 gcagcgacca gcaaggcgcc atcgccgagg agtcgccctg ccgcccgccg gctatcgtcg
       61 agcgagaggg cggccagccg ctcattgcgc gtggcgatgt agtccatgcc catgtcctcg
      121 acctgaagct cgaccaggtt gccggcgatc ttgcgcgagg tatcgaaacg cagcgcatac
      181 gagccgatca ggaacttctt ggcgcgatca agttcatcgg ctgtcggccc ggattccgcc
      241 atccgcagga cttcctggcg gatgatggcc agggattcgg cggcccgttc attgctggtg
      301 gtcgtgccgg cgaggaaggc gttggcgtgc ctgccggtgg aaagctgcga ccacaccgaa
      361 tacgccagtc cgcgcttctc gcgcacctcg ttccataggc gcgaggtgaa ggcgccgccg
      421 ccgagcacat gattgacgac gaagaccgcc agaaagtcgg ggtccttgcg ccccggcgca
      481 gggcgaccga gcgccagggt cgactgcggt accgcgaccg gaacgatacg gttcgtgccg
      541 aggcccgcca tggcagtgtc ggctattgcc ggctgagcgg ctgtcgcggg cagggcgccg
      601 aagatcgtat ccagcgcctt cgacagggcc tgcggcccaa tggcgccgac aacgccgatc
      661 ctgaggcctt cgcgcgtcag gcgcgcctga tgcgacgcca ggatgtcgtc gcgggtcagc
      721 gccgtgacgc tttcgatggt gcccaacgtg ggccggccat agggatgacc cgggtaggcg
      781 gcctgctgga aagcacgcca gaccttgtag tccggatcgt tttcgtcgcg cttgaggccg
      841 gcgatgatct gcgccttgac gcgctgcacc gcatcctcgt cgaaccgcgg cgcctggacc
      901 gccagggcaa gcaggtcgac cgccgtctgc aggttctcca ccaacgtgcg caggctgcct
      961 tccagcttgt ccttggacga ggagaagctg atctccacgg cgtattcctc aagcctttcc
     1021 tggtaggctc gggcatcgaa gtcgccagcg ccttcgtcgt aaaggccgga catcaggttg
     1081 gagaggccgt tcctgtcgct cgggtcgagc gccgcgccac cgatgaaagc gaaatccatc
     1141 gagacgatcg gcacggtgta atcttccacc agccaggcct cgatgccctt cggggaaacg
     1201 acccgctgga tgttggctgc gggagcgccg acggcggcgg cggtcaggct gggcatggtg
     1261 cgttccgttc catcgtcgcg gccggatcag gccgccttct tcaggtgtcc gaccgcagcc
     1321 agccggcgct ggagatagcg gcccgcgacg tgcttgatat tcgcaatcgt gacctcggcg
     1381 atcttcgccg gccagccccg cacatcctcg acgctgccgc ccgtgcagag cgcggcgcca
     1441 tacatccgcg ccagttgcgc ttgactgtcc tgggcataga tcatgtccgc gacgagacgc
     1501 gtgcgggcac gccgcaacac cgcttcctcg actccgtgct cagcgatccc ggcgatgatc
     1561 gcatcgatct cgccatcaag ctcggcaagc gagacgccat ccgccggcac ggcataaagt
     1621 ccgaacgtgc cttgatcgag cgcatcgttg cgataccagc tggagacggt cacggccttc
     1681 tgcttctcga ccaccagctg ccggtaggcg atgcctgcgc tggaaccggc gaggatttgg
     1741 ctcagcactt ccagggcgta cgcgtcgctt ccctccgccg tggcatgcga tggggcgaga
     1801 taaagacggt agaccgtggg ctgttccacc ttctcgtcgg ccaacgtcac caaccgctcc
     1861 gcgcgcggcg gcggctcctg cggtcgcttg cgctgaggcg gctcgttgcg gcgcttgacc
     1921 gcgccatagg acgcttccgc cagcgtgcgc acggtatcgc cttcgacatc gccggcgacg
     1981 atgaggatcg cgttttcggg cgtgtagaag cgcttgtaat aggccagcgc atcatcgcgc
     2041 ccgaggcctt cgatctcgtg ctcccagccg atgatcggcg ttccataggg atgatgcgtg
     2101 tagagcgccg ccgagaggct ttcgtacagt tgcgccgcag gatcggcgtc aacatgcatc
     2161 ttgcgttctt ccaggatcac gtcgcgctcc gaggcaacct ccgcctcgtc gagcaccaga
     2221 ccgttcatgc ggtcggcctc gagttccatc accttggcta ggtgctcctt ggcgacgcgc
     2281 tggaaatatg cggtgtagtc gtgcccggtg aacgcgttct cctggccacc gagttccgaa
     2341 acgatgtcgg aaaacacgcc tttgggatat ttagcggtgc ccttgaacat cagatgttca
     2401 aggaaatggg cgatgccgga cttgccatgc ggatcatcgg ccgcgccgtt acggtaatag
     2461 accatatgcg ttacgaccgg cgcgcgatgg tccggaatga ccagcacatc catgccattg
     2521 gcgagcgtga agtggctgag agcagcggtt ttctggccgg tcaaggcgcg atttccttcg
     2581 agcgaagcaa caggaccaga catagtgacg tttgccaaag tcgcaagcgg acaaacaaaa
     2641 aagcgggcgc tgggcccgcc tctgttgcgc atcgtgagcc ggcacgaact agcggcgctg
     2701 gcttttcagc acatccagtt caaccgcgtc cgaattctgg atgggatcga tggtcgcctt
     2761 caccggggcg cccgatgcag cccggcgata cccctttggc ggctcggtca gatactggcg
     2821 tgtcggctcc tcgccgggcc gcagcgtcgg ctgctgggcc cgtgccgcgg cctgaacgcg
     2881 tccgacttca gccgggctca gatacaggtt gttgcgatca ttctgattgc ggttgatcgt
     2941 atcctgatac gtggtgggga ccccggcata gcgattgcgg cgctgcgcct cgttcagggg
     3001 cacctgtgct ccgtagctcg gatcatagga gcgatccttc gcaaccggtt gtttcgcgtc
     3061 ctcagccttg cgcttccggg ccagcacatc gggatcctgc ggccagttgg cgttgcgttc
     3121 cgcgacgggc tcctcgggcg cgcgcagccg tccaacggat ggtggcacca caagaggcgg
     3181 gcgctcacgg tactcgattg gctccttgtc gtccggcgcg atgccaatca cgcggaacac
     3241 gctggtgagc gtatccattt gggcgaaggc cggcccgcct gcgacaacca gggcggccaa
     3301 agcggcggcg cggacacatt gagacacaac catattcccg ttccccgaaa tcccaagacg
     3361 accaagcgcc gggatagtga ccattcaagg cgttttcgcg gcattccgcc cgtctttggg
     3421 ctcaggatat agcagaaggg gtacgcccag cacaatcgcc gcgtcagcaa ggttaaagac
     3481 gtaccagctg aagcttccga cgtggaaatg cacgaaatca aatacggcgc cataggcggc
     3541 gcggtcgatg gcgttgccga tggcgccgcc gacgatcagg ccgaggccga gggcggtgac
     3601 gcgcgagcgg gcgcggtaca tccagaccgc gaggaaagtc gccgccgcaa tcatgaatgc
     3661 ggtcagcagc cagcggccaa gcgccgtatc ctgctggaac aacccgtagg aaatgccgcg
     3721 gttccataca accgtgagat cgaagtaggg accgagccgc acgggatagg cggacggcag
     3781 gccgtagacg aacagcagga acagcttgct ggcctgatcg agcgccagcg tcgccagcgc
     3841 cgcgatcagg ccaagacgga agggctttct cgacacgtca gccgagcccc agggccgcgc
     3901 gctccttcag ggcggcggca tcgcgcggcg tgacgtccgg gaaatccgga tcagcggttg
     3961 tggcatcgaa atacttccat gaacgcgagc attttttccc ctgcgcagga tggacctcga
     4021 ccgccacgcc cggaacgtcc ggcagccgga atgcacccgc cgccggctcc cgaagtcctc
     4081 gtagaacatc ggaaacaatg gcgacctcgg cgatgttcac gccttcgagc gcggtgttca
     4141 atgcaggcat ttcgacatgc agcaccggcg cggcttccag cgacgagccg atgcgcttgg
     4201 cggcgcgctc cacctccagc gcgccggtga tgacgcggcg caccgccttg acctgctccc
     4261 atttctccgc cagcgcgtca tcgcgccaga aggccgggat ctcgggaaag acttcgagat
     4321 gcacggacag cgacgcgtcg gcctcgcgtt cgggatagcg ggcgatccag gcctcctcgg
     4381 ccgtgaacga gaggatcggc gcgagccatt tcgacagcgc atcgaacagc cggtcgatca
     4441 cggtcagcgc agcgcgccgc gtgcggctcg acggcgcatc gcaatagagc gtatccttgc
     4501 ggatgtcgaa gtagaatgcc gacagttccg tgttcatgaa ctgcgccagc accgagacga
     4561 cacgcttgta gtcgaaggcg gcataagcgt cgcgcacctc gccatcgagc acggcgagcc
     4621 ggtgcagcat cagccgttcc agttccggca tggcgcgctc cgggatggaa tccgaggcgt
     4681 tgaagtgcgc cagcgagccc agcatccagc gcagggtgtt gcgcatcttg cgataggtct
     4741 cgacgaagtt cttcatgatc gccggtccga tcctgagatc gtcggaataa tccgaagcag
     4801 cgacccagag gcgcaggatg tccgcgccgg actcgtccat caccttctgc ggcgcggtga
     4861 cgttgcccag cgacttcgac attttctgcc ccttctcgtc gaggcagaag ccgtgcgtca
     4921 gcacgatgtc gaagggcgcc cggccccgtg tgccgcagga ttcgagcagg gaagaatgga
     4981 accagccgcg atgctggtcc gaaccctcca gatacatcac ggtgtcgcgc ccgccgtcca
     5041 ccttgcggcg gattccggca agcccgggga aatgcaccgg gtcctccagg gtgtaggtgt
     5101 gggtcgaacc ggaatcgaac cagacgtcga ggacgtcggt cactttctca tacgcggccg
     5161 gatcgtggcc gaacggcttg aggaagcgcg agccatcgac gtcggcgaac caggcgtcgc
     5221 ccccttcctg atcgaaggcc ttcgcgatcg cctcatccac gcgggcatcg gcgagcatct
     5281 caccgctctc gcgatgcacg aacacggtga tcggcacgcc ccaggcgcgc tggcgcgaga
     5341 ccacccagtc cgggcggttc tcgatcatgc cgttgatgcg gttctcgccc gccgccggca
     5401 cccattgcgt cgccctgatc gccgccagcg agacatcgcg cagggatttg tcccccagcg
     5461 cttcgatctt gcgatccatg gcgatgaacc actgcggcgt gttgcggaag atcaccggct
     5521 tcttggagcg ccaggaatgc ggatactggt gcttcaaccg gccgcgcgct acgaggcgcc
     5581 cggcctcgat cagcgccttg atcaccgcct cgttggccgt gcccttctcg cccgtgtcgg
     5641 tgagcacgcg atggccttcg aaccccggcg cttccctggt gaaagcgccg tcggcatcga
     5701 ccgtataggg gatatgcgtg tcgatgccgc gcgccgacag cagcttgccg gaggccatcc
     5761 aaatgtcgaa gtcctcgcgg ccatggcccg gcgcggtgtg cacgaagccc gtgccggcgt
     5821 cgtcggtgac atggtcgccg tcgagcaggg ggacgtcgaa ctcgtagccg gggatgtggc
     5881 cggcaagggg atgggcgcag gtcatgccgg ccagcattcc ggccgggact gcctcccgca
     5941 aagcaagcgt gatcttcgcg ttcttcgcga catcgtcggc gagtttggtg gcgaggatgt
     6001 aacgcgcgcc gacgaccgga ccaaaggggc gctcgttcgc cgtcacctcg tagaggccgt
     6061 agtcgatgcg gttcgagaag ctgatggcgc ggttgcccgg caaggtccag ggcgtggtcg
     6121 tccagatgac gacggaagcg tccaagccgg tcttcaccgg aaacgccacc cagaccatgt
     6181 cggaggtgtg gtcgtgatat tccacctcgg cctcggcgag ggcggttttt tcaaccacgg
     6241 accacatcac gggcttggag ccgcgataaa gctgtccggt gcgggcgaat ttcatgatct
     6301 cgcgggcgat ctgggcttcc gcatggaagg ccatggtcga atagggattg tcccagtcgc
     6361 cgatgacgcc gagccgcttg aattcctcgc gctgcacgtt gagccagtgg gtggcgaagt
     6421 cgcggcactg gcgacggaac tcgaccaccg gcacctcgtc cttgttcttg cccctggcgc
     6481 ggtactcctc ctcgatcttc cactcgatcg gcaggccgtg acagtcccag ccgggcacat
     6541 agttggaatc gaagccgagc atctgctgcg acttcgtcac catgtccttc aggatcttgt
     6601 tgagcgcgtg gccgatgtgg atgttgccgt tggcgtaggg cgggccatcg tggagcacga
     6661 acttgtcacg acccttggac tgtctgcgaa gctggccgta aaggtcgttg cgctgccagc
     6721 gggcaaggat ttccggctcc ttctgcggca ggccggcgcg catcgggaac ggtgtttcgg
     6781 gcaggaacag ggttttcgag taatcgacgg agtcaggtgt cttgtcggtc atgacgggct
     6841 cggaaattgg gtgcgcggcg gcaggaaaga gcgacgaaat cggcaaaccc ggcccgcccg
     6901 cgagcgtgtg aggcgctcag cggcaggccg ggaggctaat tcgaaaagcg cgcgcgaaaa
     6961 aggacatggg ccgcctcata gcaatccggc gccgaaaaac aaaggtttgc cgcgtcagat
     7021 tttcgtctgt cgcacgattg cacgcgcttt ttccgcatcg cggttcatct gggccaccag
     7081 cgcatcgagg ctgtcgaact tctcttccgg ccggaggaag gcgaggaagg cgaccgtgag
     7141 catctgcccg tagagatcgc cggaaaaatc gaacagcacg acttccagca gcggcgggcc
     7201 gttgtcgacg gtcggcctgc gcccgaaact ggcgacgccg ccgtaggtgt tgccggcgtg
     7261 ggcgacgcgc acggcgtaga tcccgtgtcg cagcccgcac cccgcgtcga gcgccaggtt
     7321 ggcggtaggg aaccccagcg tccgcccgag cttctgcccg tgaatgaccg gccccgacac
     7381 gaaccactca tggccgagca tggcgttcgc ggcggcgatg tcgcccgccg cgagcgcttc
     7441 gcggatgcgg gtggaggaca cggccatcgc cccttcggcg acctgatcga cgatctcgac
     7501 ggcgaacccg aaccgggcac ccgccgcgcg cagcgtctcg ggcgaccctg accggcggct
     7561 gccgtagtgg aaatcggcgc ccgcaaccac gccggcgacg ccgaggcgcc cgaccaggac
     7621 ctcccgcacg aaggcctcgg cggacagccc ggcgagcgcc gcgtcgaagg tcagcacgat
     7681 cagcgcatcg acgccgaggg tcgcggccag catcgcctgc gtccgcggcg cggacagccg
     7741 gaatctcgcc acatcaggct ggaaataggc gcgcggatgc gggtcgaagg tcaggatcgc
     7801 cagcggccgg ccgaggcgcg cggccatcgc tccggcacgg ccgatcacat gcttgtgacc
     7861 gcgatgcagt ccgtcaaaat tgccgatggc gacgaccgga ccggccaggg tctccggcag
     7921 gcctgaggga tcgcgcagca ggacgggact tccgggagcc gggcctgaga cgtcgggagg
     7981 gatcgtgcgg gtcaagtgaa caacaaacgt gagcgtgcgc cgggccgggt tcggtgtggt
     8041 taacggcttt gcaacgtcgc atcgcataat ctggcggcgc actgggatca accgttgccg
     8101 tcgaccagag gtttttgagg gaaagtcgct gagatggcca tcaacacatc catgccggtt
     8161 ctggtggtcg acgactacca gaccatggtg cggatcattc gcaacctgct caagcagatc
     8221 ggcttcgagg acgtggacga cgcgagcgac ggtgtatccg cgctggagcg gctgcggcag
     8281 cggcgctacg ggctgatcat ctcggactgg aacatgcagc ccatgtccgg ctacgaactg
     8341 ctgcgccacg tgcggtccga catcgacatg cgggccctgc cgttcatcat ggtcacggcc
     8401 gaatcgaaga cggacaacgt catcgccgcc aagcgggccg gcgtttccaa ctacatcgtc
     8461 aagccgttca acgcgcagac cctgaagtcg aagatcgagg cggtctgctg cggctgacgc
     8521 cgtcaggggt gcagcgcccg cgtcgcaaag gtcggcttca gcgcctgccg cgcgaagaac
     8581 gcagcgagcg aatcggcact caggccgccg tccttcagcc cggtttcctg ggcgtggatg
     8641 ccgtcgatca cgaacagcga ttcgatgccg aaggcggcgg cgccggccac gtcggtgcgt
     8701 atggcatcgc cgatgccgag aatccggtcc cgcgtcacgg tgcgtcctag cacccgttcc
     8761 atctcgcgca gcgcgaagtc gtagaccagc gggtgcggct tgccgacaaa ggtcaccctt
     8821 ccgcccatgc gttcgtagag atcggcgatg gcgccggcgc agggcacaag cttgtggccc
     8881 cgctccacca cgatgtccgg gttggcgcag atcagctcct ggttgcgctc a
//
LOCUS       DAOMXN010000093         8915 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_4149_length_8915_cov_15.594582, whole genome shotgun sequence.
ACCESSION   DAOMXN010000093 DAOMXN010000000
VERSION     DAOMXN010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 8915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8915
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4149_length_8915_cov_15.594582"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..129)
                     /locus_tag="PK812_07955"
     CDS             complement(<1..129)
                     /locus_tag="PK812_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017965936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRJ69526.1"
                     /translation="MKDFAGRTAVITGAASGIGLELAREAAGRGMNLVLADLDQARL"
     gene            complement(377..1006)
                     /locus_tag="PK812_07960"
     CDS             complement(377..1006)
                     /locus_tag="PK812_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015930476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRJ69527.1"
                     /translation="MSDEELHSLATIVDPRCVPAKTTLFSQGDEATNVFNVTEGMARL
                     YKLLPDGRRQIIGFALPGDFLGLSLSDRFSFSADAVDDVRVCRFQRTRFSQQLDEKPH
                     LLRRLHEIASNELMIAQDQLVVLGRRSAEERVAVFLLALRKRFAAIHHLSPTVPLPMS
                     RQDIADYLGLTIETVSRTFSKFFKEKTLLNVPDGVRILNFERLNELTQR"
     gene            1234..1683
                     /locus_tag="PK812_07965"
     CDS             1234..1683
                     /locus_tag="PK812_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69528.1"
                     /translation="MEGAFEARPICRHSLAAAVAVLRLGVPDASVQACKALCQRDRRG
                     GPKPGALMGVFDRRDYVHAVFRARMEPVLQGGSRLKIYDLVLSDAIAALFVSAIVDAL
                     EGYARAMGCDHLTVAAPQGNEFNSVPLPSVLESRGFGHHGLLMGRRL"
     gene            1998..3656
                     /gene="ccoN"
                     /locus_tag="PK812_07970"
     CDS             1998..3656
                     /gene="ccoN"
                     /locus_tag="PK812_07970"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HRJ69529.1"
                     /translation="MAPTMTGRKTLGAGEGSLAVFFALFALLWLFIASKTTDAAYAFH
                     AWLFVLGSIVAVVAIFKRYMARPAEAPPQEIDGLPNYNFGPIKFASVMAMFWGIAGFL
                     VGDIIAWQLAFPALNFDLPWTNFGRLRPLHTSAVIFAFGGNVLLATSFYVVQRTCRAR
                     LAGDLSPWFVVLGYNFFILVAGTGYLLGITQGKEYAEPEWYADLWLTVVWVVYFLVFV
                     LTVAKRKEPHIFVANWFYIAFILTIAVLHLGNNATVPVSIFSPKSVIVWSGVQDAMFQ
                     WWYGHNAVGFFLTAGFLAIMYYFVPKRANRPIYSYRLSIIHFWALIFLYIWAGPHHLH
                     YTALPDWAQTLGMTFSVMLWMPSWGGMINGLMTLQGAWDKLRTDPVLRMMVVSVAFYG
                     MSTFEGPLMSIKAVNSLSHYTDWTIGHVHSGALGWVGYVSFGALYCLYPWLWNKRDLY
                     SAKLVNWHFWLSTLGIVLYVSAMWVSGIMQGLMWRAYTQLGFLEYSFVETVAAMHPYY
                     VIRAIGGLLFVVGSLIMAWNLWKTVTSQALAIPARPAGAAVAAA"
     gene            3669..4427
                     /gene="ccoO"
                     /locus_tag="PK812_07975"
     CDS             3669..4427
                     /gene="ccoO"
                     /locus_tag="PK812_07975"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011996118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HRJ69530.1"
                     /translation="MATETPAKKSLWDRHKIFETNIVVLMIGCLLMVSVGGLVEIAPL
                     FYLKSTIEKVEGMRPYSPLELAGRAIYVREGCYNCHSQMVRPMRDEVERYGPYSLAAE
                     SMYDHPFQWGSKRTGPDLARVGGKYSDEWQRAHLANPRSIVPDSIMPGYPFLLKAELD
                     VDKAVAEMKVHRLLGVPYTDDQIKNAMVDLKAQVNPDDPNSDAMLKRYPKAQVRDFDG
                     NPEKLTEADALIAYMQMLGTLVDFKLYDNKANLR"
     gene            4437..4592
                     /locus_tag="PK812_07980"
     CDS             4437..4592
                     /locus_tag="PK812_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008189268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HRJ69531.1"
                     /translation="MTSTYTFLANLAQSAGLLYFMAAFLGICAYALWPRNREKFEQAA
                     AIPLKED"
     gene            4597..5472
                     /gene="ccoP"
                     /locus_tag="PK812_07985"
     CDS             4597..5472
                     /gene="ccoP"
                     /locus_tag="PK812_07985"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HRJ69532.1"
                     /translation="MAAHLEKDAVTGTSTTGHEWDGIKELNTPLPKWWLQLFYITIIW
                     SFAYFVVYPSWPLVSSYTKGVFGWSSRAAVEEDLAALRKRRGEQAAALEKASLDDIRK
                     SPDMSRIALAQGKAAFGDNCAPCHGNGGVGGKGYPNLIDDEWIWGGKLDDIHTTITNG
                     IRWTANNDTRLSDMVGFVKQGMLKKDEADAAIEYVRSIAKLDVAQGADLAKGKKVFAD
                     TCSSCHGDDGKGKPEVGAPDLTDAIWLFGSDRQSMTETVYNGRAGVMPAWTGRLDPVT
                     IKALTVYVHTLGGGK"
     gene            5469..6932
                     /gene="ccoG"
                     /locus_tag="PK812_07990"
     CDS             5469..6932
                     /gene="ccoG"
                     /locus_tag="PK812_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HRJ69533.1"
                     /translation="MNDIKNALEHETHIEGPLYAAAKRIYPQRVHGLFRNIKWYTLLF
                     CLGVYYFLPFVRWDRGPNLPNQAVLLDLQNRRFYFFFIEIWPQEIYYFTGLLIIAALV
                     LFLMNSIGGRIWCGYLCPQTVWTDLFMAVERHIEGDRRDRIKLDAEPWHAQKVIKKVA
                     KHTIWLGIAWWTGGAWVLYFADAPTLVKDLVHFEAPLTAYVWIGILTFTTYALAGHMR
                     EQVCLYMCPWPRIQAALTDEKALNVTYRYDRGEPRMSVKEAAKAKAAGGPAGDCVDCM
                     QCVNVCPTGVDIRDGSQLGCIQCGLCIDACDTIMTKIGRPTRLIAYDTDDAIAARIKG
                     EPYTYKFIRARIVLYVAIIAFVGGLMLYQLATRSTAGISILHDRQPLFVTLSDGSIRN
                     AYTMRLLNKRPDNRQFAITLEGPLGAAIEVVGVPATADWRPIVEVGPDQTREVRLLIT
                     VPRDAVKERSQLITIKAADLFAGERVSAVDHFIGPGP"
     gene            6978..7502
                     /locus_tag="PK812_07995"
     CDS             6978..7502
                     /locus_tag="PK812_07995"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HRJ69534.1"
                     /translation="MPTELVPAARSGGKPPREITGRFVLIALILFFGVVATVNFTMMT
                     LATRTFPGADARNGYDVSQGYNREIATARAQAERGWKADVELQRTNGVATLTFRVRDG
                     ASNPVSGLAAEARLRHPSDRRQDHVMALSETSAGVYQARENLSLTGAWDVAVTARSQG
                     DKVFSSQSRIILKD"
     gene            7507..>8915
                     /locus_tag="PK812_08000"
     CDS             7507..>8915
                     /locus_tag="PK812_08000"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRJ69535.1"
                     /translation="MSAVALGEAPKDLSLFVRPGEAGGEAMDLAVAGIKCAGCMATIE
                     KGLASEPGIVSARVNLASKRLTVEWRKGQNDPARIIERLDELGFRAHPFSPRKLDTEE
                     AREEKHLLRCLGVAAFASMNIMLLSVSVWSGNATDITPETRDFFHWISALIALPTAAY
                     AGRPFYESAIKAIRARAVNMDVPITLGILLALTMSVVETFNHAEHAYFDSAVMLIFFL
                     LIGRYLDQATRRKTREIAGNLATLRAETATKIAANGTLVEVPVDAVDPGDLVLASPGQ
                     RIAVDGLIEDGASEVDQSLVTGETRHVAVTKGDSVYAGTLNVSGQLRIRVTKAESGTL
                     LAEIERLLTRAIEGRSHYVRLADRAARLYAPVVHATALISFLGWILFGLAWQKALIIA
                     ITVLIITCPCALGLAIPAVQVVATQALFRRSVLLHAPDALERLSEVDTIVFDKTGTLT
                     LPVPALANAAAVSGDDLALA"
BASE COUNT         1532 a         2793 c         2880 g         1710 t
ORIGIN      
        1 gagtcgcgcc tggtcgagat cggcgagcac gaggttcatg ccccgtcccg ctgcctcccg
       61 cgccagctca agcccgattc cgcttgctgc gccggtgatc acggccgtcc ggccggcaaa
      121 gtccttcatg tcgcgtttcc gtccccggtc ctcgccacaa gcggcgacat actcgcgagt
      181 atgttagtgc catccaagag ggatgcaagg cggaattgtg cctccggtga gcgtggaggg
      241 cagcccagcc ggtccaagcg cggcgcgcaa gaacgctggc catgtcggcg gcaaatgcgg
      301 agatgctttt gctgggagcg aaaacgcacc gtgcctgacg gcggcgcccg aagacctgtc
      361 gccgcgtccg ccgggatcag cgctgtgtca gctcgttcag tctctcgaag ttgaggatcc
      421 gtacgccgtc cggcacgttc agcagggtct tttccttgaa gaacttggaa aaggtgcggc
      481 tgacggtctc gatggtcagg ccaaggtaat cggcgatgtc ctgccggctc atcggcagcg
      541 gcacggtggg cgacaggtgg tgaatggcgg caaaccgctt gcgcagggcc agcaggaata
      601 ccgcgacgcg ctcctcggcg ctgcgcctgc caagcaccac gagctggtcc tgcgcgatca
      661 tcagctcgtt cgaggcgatc tcgtgcaggc gacgcagcag atgcggcttc tcgtcaagct
      721 gctgcgagaa tcgggtgcgc tggaaacggc acacgcgcac gtcgtcgacg gcatctgccg
      781 aaaagctgaa acgatccgag aggctcagcc ccaggaagtc gccgggaaga gcaaacccga
      841 tgatctggcg ccggccatcc ggcaggagct tgtagagccg tgccatgccc tcggtgacat
      901 tgaagacgtt tgtcgcctcg tcgccctggc tgaacagcgt ggtcttggcc ggtacgcagc
      961 gcggatcgac aatggtggcc aggctgtgca gctcctcgtc gctcaatgcg ttgcagacgc
     1021 tcaggaaacg agcgcggcaa tgctgacagt ccacctgcag ttcagaacct gcacagggcg
     1081 gatgcctctg caatgcgcct gccgtcacca tgctgactcc ctctttaccg gtctgtccgg
     1141 ctgcatatgc ccaccttgtt tgactcaggt caatgagcgt gtggcgcgcg catgggagcg
     1201 atgccgctct ttcgagttgg gggactgcgc cttatggagg gagcgttcga ggcgcggcca
     1261 atctgccggc actcactggc ggccgccgtc gctgttttgc ggcttggcgt tcccgatgcc
     1321 tccgtgcagg cgtgcaaggc gttgtgccag cgcgatcgcc gcggcggtcc caagccgggc
     1381 gccctgatgg gtgtctttga tcggcgcgac tatgtccacg cggtgttccg ggcacgcatg
     1441 gaaccggtgt tgcagggcgg gagtcggttg aaaatctatg accttgtgct gtcagatgcg
     1501 attgccgcgc tctttgtctc cgccattgtc gacgccctcg aagggtatgc acgcgcgatg
     1561 gggtgcgatc acctgaccgt cgccgcgccg caaggcaacg agttcaacag cgttcccctg
     1621 cccagtgtgc tggagtcgcg cgggtttggc catcacggct tgctgatggg gcgtcggctc
     1681 tagcatgggc cccatatttt agactctgct gatggatgtg acgatttgtc tcggacacgg
     1741 cgccggcccg gcgggcgaac aagggccgct acccaacgcc ttgaatatgc aggctaaccg
     1801 ttcctgagcc gcgcgccgga gcacgcggtg cgcggcccgt gcgtcgtcct gacgcgtggc
     1861 tggaacgatc agaccgaagt tcaagccgcg gccaggcagc cggttgattt gtgtcaagga
     1921 gtgccaaggc ccgtgcgggc agaagggcat gatgagactc tagcggcatc gggcgtattc
     1981 caacctgggg aaagaaaatg gctccaacaa tgacgggtcg aaagaccctg ggggcgggag
     2041 agggcagtct ggctgtcttc ttcgcccttt ttgcgcttct gtggcttttc atagcctcga
     2101 agacgaccga tgcggcctat gcgtttcacg cgtggctgtt cgtgctcggc agtatcgtgg
     2161 cggtggtcgc gatcttcaag cgctacatgg cgcgtccggc cgaggcccct ccgcaggaga
     2221 tcgacggtct gccgaactac aacttcggcc cgatcaagtt tgcctccgtc atggccatgt
     2281 tctggggcat cgcgggtttc ctggtcggtg acatcatcgc ctggcagctg gccttcccgg
     2341 cgttgaactt cgacctgcct tggacaaact ttgggcgcct gcgtccgctg catacctcgg
     2401 cggtgatctt cgccttcggt ggcaacgtgc tgctggcgac gtcgttctac gtggtgcaac
     2461 gcacctgccg cgcgcgactg gctggcgacc tttcgccctg gttcgtggtg ctgggctaca
     2521 acttcttcat cctggtggcc ggcacgggct atctgctcgg catcacgcag ggcaaggagt
     2581 acgccgagcc cgaatggtat gcggacctgt ggctgaccgt cgtctgggtg gtctacttcc
     2641 tggtgttcgt gctgacggtc gccaagcgca aggaaccgca catcttcgtg gcgaactggt
     2701 tctacatcgc cttcatcctg acgatcgccg tgctgcatct gggcaacaac gccacggtgc
     2761 cggtgtcgat cttctcgccc aagagcgtga tcgtctggtc gggcgtgcag gatgcgatgt
     2821 tccagtggtg gtacggccac aacgcggtgg gcttcttcct gaccgccggc ttcctcgcca
     2881 tcatgtacta cttcgtgccg aagcgggcga accggccgat ctactcctac cgcctgtcga
     2941 tcatccactt ctgggcgctg atcttcctct acatctgggc aggtccgcac catctgcact
     3001 acacggcgct gccggattgg gcgcagacgc tgggcatgac cttctcggtg atgctgtgga
     3061 tgccttcgtg gggcggcatg atcaacggcc tgatgacgct gcagggcgcc tgggacaagc
     3121 tccggaccga tcccgtgctg cgcatgatgg tcgtgtcggt cgccttctac ggcatgagca
     3181 ccttcgaagg cccgctgatg tccatcaagg cggtcaactc gctgagccac tacacggact
     3241 ggaccatcgg ccacgtgcat tccggtgcgc tcggctgggt gggctacgtg tccttcggcg
     3301 cgctctactg cctgtatccc tggctgtgga acaagcgtga cctctactcc gccaagctgg
     3361 tgaactggca cttctggctg tcgaccctgg gcatcgtgct ctacgtctcg gccatgtggg
     3421 tttccggcat catgcagggt ctgatgtggc gcgcctacac ccagctcggc ttcctcgagt
     3481 actcgttcgt cgagaccgtg gccgcgatgc atccctacta tgtcatccgc gccattggcg
     3541 gcctgctctt cgtggtcggc tcgctgatca tggcctggaa cctctggaaa accgtcacgt
     3601 cccaggccct cgccatcccg gctcgtccgg cgggcgcggc tgtggccgca gcgtgaggag
     3661 aaacgacaat ggcaactgaa acaccagcca agaagtcctt gtgggatcgc cacaagatct
     3721 tcgaaaccaa catcgtcgtc ctcatgatcg gctgtctcct gatggtctcg gtcggtggcc
     3781 tggtcgagat cgctccgctc ttctacctga agagcacgat cgagaaggtc gagggcatgc
     3841 gaccctacag cccgctcgaa ctggcgggcc gggcgatcta cgtccgcgag ggctgctaca
     3901 actgccacag ccagatggtc cgcccgatgc gcgacgaggt cgagcgctac ggcccctact
     3961 cgctggcggc ggagagcatg tacgatcacc cgttccagtg gggctccaag cgcaccggtc
     4021 ccgatctggc gcgcgtcggc ggcaagtact ccgacgagtg gcagcgggcg catctggcca
     4081 atccgcgctc gatcgtgccg gactcgatca tgccgggcta tccgttcctt ctgaaggcgg
     4141 agctcgacgt cgacaaggcc gtagcggaga tgaaggtgca ccgtctgctg ggcgttccct
     4201 acacggatga ccagataaag aacgcgatgg tggacctgaa ggcgcaggtg aatccggacg
     4261 atccgaactc ggacgcgatg ctcaagcgct accccaaggc gcaggtgcgt gatttcgatg
     4321 ggaatcccga gaagctcacg gaggccgatg cgctcatcgc ctacatgcag atgctcggca
     4381 ccctggtcga cttcaagctc tacgacaaca aagccaacct gaggtgagga cggaccatga
     4441 ctagcaccta cacgttcctt gccaatttgg cgcaatcggc tggactgctc tacttcatgg
     4501 cggccttcct cggcatctgt gcctacgcgc tctggcctcg caatcgcgag aaattcgaac
     4561 aagcggcggc gatcccgctg aaggaggact gatcccatgg ctgcgcactt ggaaaaggac
     4621 gccgtcaccg gcacgtcgac gaccggtcac gaatgggacg gcatcaagga actgaacacg
     4681 ccgctgccga agtggtggct ccagctgttc tacatcacga tcatctggtc gttcgcctac
     4741 ttcgtggtct acccatcctg gccgctggtc tcgagttaca cgaagggtgt cttcgggtgg
     4801 tcgtcgcggg cggcggtcga ggaagacctg gccgcgctcc gcaagcggcg cggcgaacag
     4861 gcggcggcgc tcgaaaaggc ctcgctggac gacatccgca agagccctga catgtcgcgc
     4921 atcgctctgg cccagggcaa ggcggccttc ggcgacaatt gcgcgccgtg ccatggcaac
     4981 ggcggcgtcg gcggcaaggg ctatccgaac ctgatcgacg acgagtggat ctggggcgga
     5041 aagctggatg acatccacac gacgatcacc aacggcatcc gctggaccgc caacaacgac
     5101 acccgtcttt cggacatggt cggcttcgtg aagcagggca tgctgaagaa ggacgaggcg
     5161 gatgcggcca ttgagtatgt ccgctccatc gccaagctgg acgtcgccca gggcgccgat
     5221 ctggcgaaag gcaagaaggt gttcgccgac acctgctcct cgtgccacgg cgacgatggc
     5281 aagggcaagc cggaggtcgg tgcgcctgac ctgaccgatg ccatctggct gttcggatcg
     5341 gatcgccagt ccatgaccga gacggtatac aacggtcgcg cgggcgttat gccggcctgg
     5401 acgggccgtc tcgatccggt gaccatcaag gcgctcaccg tctacgtgca tacactcggt
     5461 ggtggcaagt gaacgacatc aagaacgcgc tggagcatga aacgcatatc gagggtccgc
     5521 tttatgcggc cgcgaagcgg atttaccccc agcgcgttca tggtctcttt cgcaatatca
     5581 agtggtacac gctgctgttc tgcctcggcg tgtactactt tctgccgttc gtgaggtggg
     5641 atcgcggccc caaccttccg aaccaggccg tcctgctgga tttgcagaac cggcgcttct
     5701 atttcttctt catcgagatc tggccgcagg aaatctacta ctttaccggg ttgctgatca
     5761 ttgccgccct ggtcctattc ctgatgaact cgatcggcgg gcgcatctgg tgcggctacc
     5821 tttgcccgca gacggtgtgg acggacctgt tcatggcggt cgagcgccac atcgagggtg
     5881 accggcgcga ccgcatcaag ctggacgccg agccctggca tgcccagaag gtcatcaaga
     5941 aggtcgcgaa acacacgatc tggcttggca ttgcctggtg gacgggcggc gcctgggttc
     6001 tctatttcgc cgacgcgccg acgctggtga aggacctggt ccatttcgag gcaccgctga
     6061 cggcctatgt ctggatcggc atcctgacct tcacgaccta cgcgctggcc ggtcacatgc
     6121 gcgaacaggt ctgcctctac atgtgtcctt ggccgcgcat ccaggcggcg ctgaccgatg
     6181 agaaggcgct caacgtcacc tatcgctatg atcgcggcga gccgcgcatg tccgtcaagg
     6241 aggctgcgaa agccaaggcg gcgggcgggc cggcgggcga ctgcgtcgac tgcatgcaat
     6301 gcgtgaatgt ctgcccgaca ggcgtcgaca tccgggacgg ttcgcaactg gggtgcatcc
     6361 agtgcgggct gtgcatcgat gcctgcgaca cgatcatgac gaagatcggt cgcccgacac
     6421 ggctcatcgc ctacgacacg gacgatgcga tcgccgcccg gatcaagggt gaaccctaca
     6481 cgtacaagtt catccgcgcc cgcatcgtcc tctatgtggc gatcatcgcg ttcgttggcg
     6541 gattgatgct gtatcagctc gcgacccgtt cgaccgctgg catcagcatc ctgcatgacc
     6601 gacagccgct gttcgtgacg ctctcggacg gctcgatccg caacgcctat acgatgcggc
     6661 tcttgaacaa gaggccggac aaccgccagt tcgcgatcac gcttgaaggg cctctcggcg
     6721 ccgcgatcga ggttgtcggt gtgccggcga ccgcggactg gcgtcccatc gtcgaggtgg
     6781 ggccggacca gactcgcgag gtccgcctgc tgatcacagt gcccagggac gccgtgaaag
     6841 agcggtcgca attgataaca atcaaggcgg ccgacctgtt cgcgggcgaa agggtaagcg
     6901 ctgtggatca ttttatcggg ccgggaccat gaccggcatt gaggccaacc gtgcaatcct
     6961 gctgcggagt gatgatcatg ccgactgaac tcgtccctgc cgccaggagc ggcgggaagc
     7021 cgccgcgcga gatcacgggg cgctttgtcc tgatcgccct gatcctgttt ttcggcgtgg
     7081 tcgcgaccgt caatttcacc atgatgacgc tggcgacgcg cacctttccc ggcgcggacg
     7141 cccgcaatgg ctacgacgtg agccagggct acaatcggga gatcgccacg gcgcgggcac
     7201 aggccgagcg aggctggaag gcggatgtgg agttgcagcg caccaacggc gtggccacgc
     7261 tgactttccg cgtacgcgac ggggcgtcca atcctgtctc gggccttgcg gcggaagcgc
     7321 gcctgcgcca cccgtcggac cggcggcagg atcatgtgat ggcgctgtca gagacttcag
     7381 cgggcgtcta tcaggcgcgc gaaaacctgt cgctcaccgg tgcctgggat gtcgctgtca
     7441 cggcgcgttc gcagggcgac aaggtgtttt cgtcgcaaag ccgcatcata ctgaaggact
     7501 gagccgatgt ccgctgtcgc tttgggggaa gcgccgaagg acctgtcgct cttcgttcgg
     7561 ccgggagagg ccggcggcga agccatggac ctggccgttg ccggcatcaa gtgcgccggc
     7621 tgcatggcca cgatcgagaa gggtcttgca agcgagcctg gcatcgtttc agcgcgtgtc
     7681 aatctggctt ccaagcgcct gacggttgag tggcgcaagg gacagaacga tccggcgcgc
     7741 atcattgaac ggctcgatga actcggattc cgagcgcatc cgttttcccc gcgcaagctc
     7801 gacaccgaag aggcgcgcga ggaaaagcac ctgctgcgct gcctcggcgt ggctgccttc
     7861 gcctcgatga acatcatgtt gctgtcggtg tcggtgtggt ccggcaacgc gacggacatc
     7921 acgccggaaa cccgggattt cttccactgg atctcggcgc tgatcgccct gccgacggcg
     7981 gcctatgccg gacggccgtt ctacgagagc gcgatcaagg cgatcagggc gcgggcggtg
     8041 aacatggatg tgccgattac cctcggcatt ctcctcgccc tgaccatgtc ggttgtcgaa
     8101 acgttcaacc acgcggaaca cgcctatttc gacagcgccg tgatgctgat ctttttcctg
     8161 ctgatcgggc gctacctcga ccaggcgacc cgccgcaaga cccgggagat cgccggcaat
     8221 ctggcgacgc tgcgtgccga gacggcgacg aagatcgccg cgaacgggac gctggtcgaa
     8281 gtgccggtgg atgcggtcga ccccggcgat ctggttctgg ccagccccgg ccagcgcatc
     8341 gccgtggacg gattgatcga agacggggcc tcggaagtcg accagagcct tgtcacgggc
     8401 gagacgcgcc acgtcgccgt caccaagggc gattcggtct atgccggcac gctcaacgtc
     8461 tcgggccagt tgcgcatccg cgtcaccaag gcggagagcg gcacgctgct ggcggagatc
     8521 gagcgcctgt tgacgcgcgc cattgaggga cggtcgcact acgtgcgtct ggcggatcgg
     8581 gcggcgcggc tctatgcgcc tgtcgtccat gccacggcgc tgatcagctt cctcggctgg
     8641 atcctgttcg gactggcctg gcagaaggcg ttgatcatcg ccatcaccgt gctcatcatc
     8701 acctgtccct gtgcgctcgg tctggccatt cccgcggtgc aggtggtcgc gacgcaggcc
     8761 cttttccgcc ggtcggttct tctgcatgcg cctgatgcgc tggagcgctt gtccgaggtc
     8821 gacaccatcg tcttcgacaa gaccggaacg ctgacgctcc cggtcccggc gctcgccaac
     8881 gcggcggccg tctccggcga cgatctggcg cttgc
//
LOCUS       DAOMXN010000094         8781 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_4217_length_8781_cov_10.054779, whole genome shotgun sequence.
ACCESSION   DAOMXN010000094 DAOMXN010000000
VERSION     DAOMXN010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 8781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8781
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4217_length_8781_cov_10.054779"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4353)
                     /locus_tag="PK812_08005"
     CDS             complement(<1..4353)
                     /locus_tag="PK812_08005"
                     /inference="COORDINATES: protein motif:HMM:NF013600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69536.1"
                     /translation="MAIQKKSSDPTEAALSAIEEALTGLPAGTKPEAARETPPRLPSV
                     NEGDDAFARELGRVAETKVRNAASKPATSDAPGLTVTTALEPAAPASAQDQTPTEAPA
                     PRFAANDDRVSVQALLGALQGKPSRAPIVWAVLASLLWLAGTALYLSQPGGFFVAPDG
                     ALRPFSATPAILATVAVLLPITLFFVLAAVQRRAQEMRSAAQALGLIAARLSEPESIA
                     SEKVFTLGQAIRREVASMGDGIERAIGRAGELETLVHSEVSALERSYSENELKIRSLL
                     NELASQRDALNANAERVRIAMLSAEEGMSHQLASMSTSLVANVGDIASRVSMQLDEKR
                     NEVTQTLSAASDAIVERLSSHSGDVIQRLSSAGEDVSSRIAATGEDVNGRLAQTGGEI
                     AARLAETGASLTQEIADRGSSVAASLKSTGETIQSAFAESAQIVGESFETGSRTLLEA
                     LSARVEEVDVSLRATGEGLTARISTVSQEATERLAARGAEITEQVEAASVRAAEIIVA
                     RGTDMAQGIESAGNAIADMIATNGEVVTRGLETASSRLHHLIQTDGHSLSDRLAEISA
                     RVEKAVAIDGKRLEENIDSAAIRVSDVIADRVGQMSEAFVLAGEHLTTQLSTHVEQTR
                     EAYALAGEHLSTQLLTHAEQAREAFAQHAETVHEDFRRKTQDTTAHFSEAAQDIAFAL
                     QTQGTRVNETLAETARRLADEVQDRTAGLSERFSAFEKAFVERAVNLEQLVLVHGSTM
                     NEQLQNHLATFEERATAKTADVASTLSQLVASAESNLGERASVLAREVAAFEASTSQK
                     AAEISERLSALMERAEGGLDLRGRQLSESLAQRTVEFAKTLAQGTDALQSSLTGRIDE
                     IDQATSRLNTVVAQRSDELAKTMAQGSDAMQSALSGRVDEIGRAVAEQSQLLAARLGQ
                     HVGAVTTELAAQSEAIAATLGARTAELSGVLSTQASTFESDVIARLEALSTSFESRGA
                     LLVRSLAEKADEAGQSLLTRASEVDRILLQVSQVASRTLISTADEVSGRLGANTEELR
                     KLFTDESASIVAQSDALAEKMAAHQEHFATRASELAGSFERHGSRFVEALEGRGMEVA
                     ASIARSGNAAAEQLTRSLADLETAVQAGAGKSVNALEEAQTRLRGEVSMVLGRLSDAN
                     NVLNTILANATQGLTSVETDLAARVARLEATLNAIVSATTDGTRQLADQVDALRGASE
                     DAVDRSATLVERLGDRTRILQDATRTLTLAHQQADEAIAERQREMDRIAASLAERTEA
                     LDASMRAFASVVDETLQSVEGRTRHVATSLSDNIRNAVQTVAQQFTSLRSQSGEEALR
                     VQEELSAAYSRTLGEAETLFRRVTEGFGETATQLRSTAGDVLRELEATRAELKHSTAE
                     LPRQTLEATASVRRAVSDQVKVLQELSGIVARSGGAMDVVAPAATPSALRAPVHEEPV
                     APVEKPQAESVIATL"
     gene            4626..5009
                     /locus_tag="PK812_08010"
     CDS             4626..5009
                     /locus_tag="PK812_08010"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HRJ69537.1"
                     /translation="MFSSDQSATTKMGGIASFERPIDLVHLARQTLGDRALEQEILSL
                     YVKQARSLLARIEQCGCGRDRSDLAHTLKGSSRAVGAWQVAQAAEAVEIVPPGRDAEF
                     DAALKALHTCIEAACDTIGDLQRAH"
     gene            5142..5462
                     /locus_tag="PK812_08015"
     CDS             5142..5462
                     /locus_tag="PK812_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRJ69538.1"
                     /translation="MPKITFVAADGTRRTVEAENGSTVMEAAIKNGVPGIEAECGGAC
                     ACATCHVYVDAEWTAVTGKPEPTEEDMLDFAFDIRPNSRLSCQIKVSDKLDGLVVQTP
                     TKQA"
     gene            complement(5515..6759)
                     /locus_tag="PK812_08020"
     CDS             complement(5515..6759)
                     /locus_tag="PK812_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008548131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRJ69539.1"
                     /translation="MSARRARPEFFAMPQTSRSPLAFLMALNFINFLGFAGWSVLFNN
                     FAKESAGFGGLEIGIVQSVREIPGFLAFTAIAWFAVMREQTFALVALIILALGIMITG
                     FLPSLTGLVITTTIMSIGFHYSETANQSLALQLFPKAAAARMMGKVASAGAAASIIAY
                     AGMAALWWIGLTDYSILFAVLGGAGTLLTIVVWRWFPHIEGSVPQRKTIVLRSRYWLY
                     YALTLMSGARRQIFSAFAGFLLVEKFGFKLPEVALLLLATACLNMLLAPWFGSLISRI
                     GERHAIMIENVCLILVFAGYAIVDDGRVAAVLFILDGVIMTLMIAQKTYFQKIGDAAD
                     MAPTAAVAFTINHIAAVFIPFVFGLLWIKDPSLVFQSGAVIATISLVLSFIVPRDPRQ
                     GQETVFREGTGSGAAQPGSVRA"
     gene            6893..7327
                     /locus_tag="PK812_08025"
     CDS             6893..7327
                     /locus_tag="PK812_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRJ69540.1"
                     /translation="MYKKVLVPVDLADMEVASPALQRATALAAASGGELRLVNVLSLI
                     PAAYLDYMPANFDVQERERAQSDLKALAAKQGLPEGKVSSVVRLGAIYHEVLAEAKEW
                     GADLIVTGSHDPTFATYLIGSNAASIVRHAHCSVLVVRKKDV"
     gene            complement(7335..7925)
                     /locus_tag="PK812_08030"
     CDS             complement(7335..7925)
                     /locus_tag="PK812_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69541.1"
                     /translation="MDTPTLSYAYRSRPFAAETRLELGRKELIAWQAGQQYVYPYHAI
                     ATIRLFFAPRGIDMSGYRAKIYSRDGKTISLEDRTFRGMVQVERQGQAYRAFITALCR
                     RAATENPQVLLHSGRNPFMLALSGMLGMATTGTLAFLGGRAALQGQWLLAAAIIGFAG
                     LFGFWSWRYVVNNRPRRFVAAAIPEAVLPQDRPRPA"
     gene            8036..8563
                     /locus_tag="PK812_08035"
     CDS             8036..8563
                     /locus_tag="PK812_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRJ69542.1"
                     /translation="MKTLILMRHAKAEPQRGKDDFLRALAERGRTAAPLVGGFLKTNR
                     LVAERVLVSGAVRTRETYALAAAIAGLPPAEFQNDLYLAPANALLAFIRKTPARSKSL
                     MIIGHNPGLAELTQWLAAPEESDGAALFKAARKFPTASVAVFDVLTPWSEVGEGDCAL
                     KRFVTPSDLGGHDED"
     gene            8601..>8781
                     /locus_tag="PK812_08040"
     CDS             8601..>8781
                     /locus_tag="PK812_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HRJ69543.1"
                     /translation="MRNASASLFDYLGSFGQPTLRLGVTGLSRSGKTVFITALVHALI
                     SGGRLPVFRARAEGRL"
BASE COUNT         1464 a         2836 c         2985 g         1496 t
ORIGIN      
        1 cagcgtcgcg atcacgctct ccgcttgcgg cttttccacc ggcgcgacgg gttcctcgtg
       61 caccggagcg cgaagcgcag acggcgtggc cgcaggcgcg acgacatcca tggcgccgcc
      121 cgagcgcgca acgatgccgc tcagttcctg caggaccttg acctgatccg agaccgcgcg
      181 gcggacggat gccgtcgcct ccagggtctg gcggggcagt tccgccgtcg agtgcttgag
      241 ttcggcgcgc gtggcttcca gttcccgcag tacgtcaccg gcggtggagc gcaactgggt
      301 ggcggtctcg ccgaagcctt cggtcacgcg gcggaacagc gtttccgctt cgcccagggt
      361 cctggaatag gcagcggaca gctcctcctg gacacgcaga gcctcctcgc ccgactgcga
      421 acgcagggag gtgaactgct gcgcgacggt ctgcaccgcg ttgcggatgt tgtcggaaag
      481 cgaggtcgcg acgtggcgag tgcgcccttc cacgctctgc agggtctcgt cgacgaccga
      541 ggcgaacgcg cgcatcgagg catccagcgc ttccgtacgc tcggcgagcg aggcggcgat
      601 ccggtccatt tcgcgctggc gctcggcgat ggcctcgtcg gcctgctgat gcgcgagcgt
      661 cagtgtgcgg gtcgcgtcct gcaggatgcg cgtgcgatcg ccaagacgct caaccagcgt
      721 ggcggagcgg tccaccgcgt cttccgaggc gccacgcagg gcatcgacct gatcggcgag
      781 ctgacgggtg ccgtccgtgg tggcggagac gatggcgttc agcgtcgctt ccagccgggc
      841 gacccgggcc gccagatcgg tctcgacact ggtcaagcct tgcgtggcat tggccaggat
      901 ggtgttgagg acgttgttcg catcgctcag gcgtccgagc accatgctga cttcgccgcg
      961 cagcctggtc tgggcttcct caagcgcgtt gaccgacttg ccggcgccgg cctgcacagc
     1021 ggtctccagg tccgcgaggc tgcgggtcag ctgctccgcc gccgcgtttc cgctgcgtgc
     1081 gatggaggcg gcgacttcca tgccgcggcc ttcgagcgcc tcgacaaagc ggcttccgtg
     1141 gcgctcgaag ctgccggcca gctcgctcgc ccgcgttgcg aagtgttcct gatgcgcggc
     1201 catcttttcg gcgagcgcgt cagactgggc aacgatgctg gcggactcgt cggtgaacag
     1261 cttgcgcagt tcctcggtgt tggcgccgag acggccggaa acctcgtccg ccgtggaaat
     1321 cagcgtgcgg ctggcaacct gcgagacctg aagcaggatg cgatccacct cgctcgcgcg
     1381 ggtcagcagg gactggcccg cctcgtcggc cttctcggcc agcgagcgga caagcaaggc
     1441 accacggctc tcgaacgagg tgctgagcgc ctccagcctg gcgatgacgt cgctctcgaa
     1501 ggtcgaagcc tgggtcgaca gcacgccgga cagttccgca gtacgtgcgc cgagtgtcgc
     1561 ggcgatcgcc tcggactgtg cggccagttc cgtcgtcacg gcgccaacat gctggccgag
     1621 gcgcgcggcc agcaactggg actgctccgc cacggcgcgg ccgatctcgt caacgcgtcc
     1681 ggacagcgcg gactgcatgg catcgctgcc ctgcgccatg gtcttcgcga gttcgtcgga
     1741 acgctgcgcg accaccgtgt tcaggcgtga tgtcgcctgg tcgatctcgt cgatgcggcc
     1801 ggtgagcgag gattgcagag cgtcggtgcc ctgcgccagg gttttcgcaa actccaccgt
     1861 gcgctgtgcc aagctctcgc tgagctgacg tccgcgcagg tccagcccgc cttccgcccg
     1921 ttccatgagc gcggacaggc gctccgaaat ctcggcggcc ttctggctcg tcgatgcctc
     1981 gaaggccgcg acctcgcgtg cgagcacgct ggcgcgctcg ccgaggttgg attcggccga
     2041 cgccaccagc tgcgaaagcg tgctggccac atcggctgtc tttgccgtgg cgcgttcctc
     2101 aaaggtggcg agatggttct ggagctgctc gttcatggtc gagccatgga ccagcaccag
     2161 ctgttcgagg ttgaccgcgc gctccacgaa ggccttttcg aaggcgctga accgctcgct
     2221 gaggccagcc gtgcgatcct ggacctcgtc ggcaagccgt ctcgccgtct cggccagcgt
     2281 ctcgttgacg cgcgtgccct gggtctgcag cgcgaaggcg atgtcctgtg ccgcttcgga
     2341 gaaatgcgcc gtcgtgtcct gcgtcttgcg gcggaagtct tcgtgcacgg tttcggcgtg
     2401 ctgagcgaag gcttcgcgcg cctgctcggc atgggtcagc aactgcgtgg acagatgctc
     2461 gccagccagg gcgtacgcct cgcgcgtctg ctcgacatgg gtcgaaagct gagtggtcag
     2521 gtgttcgccg gccagaacga aagcctcgct catctggccg acccgatcgg cgatcacgtc
     2581 ggagacgcgg atcgccgcgc tgtcaatgtt ttcctcgaga cgcttgccgt cgatggcgac
     2641 cgccttctcg acccgcgcgc tgatttcggc cagccgatcg gacaggctgt gtccgtcggt
     2701 ctggatcaga tgatgcaggc ggctgctggc cgtctccagc ccgcgcgtca cgacctcacc
     2761 gttggtggcg atcatgtcgg cgatggcatt gccggccgat tcgatgccct gggccatgtc
     2821 cgtgccgcgc gccacgatga tctcggcggc gcggacgctg gcggcttcga cctgctcggt
     2881 gatttctgcg ccacgcgcag caagccgctc cgtcgcctcc tggctgacgg tgctgatcct
     2941 ggcggtcagc ccttcgccgg tggcgcgcag ggagacatcc acttcttcga cgcgcgcgct
     3001 gagtgcttcc aacagcgtcc tgctgcccgt ttcgaaggat tcgccgacga tctgcgccga
     3061 ttccgcgaag gcggactgga tggtttcgcc cgtggacttc aagctggccg ccacggaaga
     3121 gcccctgtcg gcgatctcct gcgtcaggct tgccccggtt tccgccagcc ttgcggcgat
     3181 ctcgccgccg gtctgggcga ggcggccgtt cacgtcctcg ccggttgcgg cgatacgcga
     3241 tgaaacgtcc tcgcccgcgc tggaaagacg ctggatgaca tccccggaat gggacgacag
     3301 gcgttcgacg atggcgtccg acgcggcgga cagggtttgc gtcacctcgt tgcgcttctc
     3361 gtcgagctgc atggacacgc ggctggcgat gtcgccgaca ttcgcgacaa gagaggtcga
     3421 catggacgcc agctgatgcg acatgccttc ctcggccgac agcatggcga tgcgaacgcg
     3481 ctcggcattg gcgttcagcg catcgcgctg cgaggcgagt tcattgagaa gcgaacggat
     3541 cttgagttcg ttctcgctgt atgagcgctc cagcgccgag acttcggaat gcacgagcgt
     3601 ttccagttcg ccggcgcgtc cgatggcccg ctcgatgccg tcgcccatcg aggcgacttc
     3661 gcggcggatg gcctggccga gcgtgaacac cttctcgctc gcaatcgatt ccggctcgga
     3721 cagacgcgcc gcgatcaggc ccagggcctg ggcggcggag cgcatctcct gggcgcggcg
     3781 ctggaccgcc gcaagcacga agaacagcgt gatcggcagc agcacggcga cggtggcgag
     3841 gatagcgggc gtcgcggaga agggacgcag ggcgccatcc ggcgcgacga agaaaccgcc
     3901 cggctgggaa agatagagag cggtaccggc gagccagaga agcgaagcga ggaccgccca
     3961 gacaatcggc gccctggagg gcttgccctg caacgctccg aggagcgcct ggaccgagac
     4021 gcggtcatca ttggcggcga aacggggggc gggtgcttcg gtcggtgtct gatcctgcgc
     4081 actggcgggc gccgcaggct ccagggccgt tgtcaccgtc aggccggggg cgtcggaggt
     4141 cgccggcttg ctggcggcgt tgcgcacctt ggtttccgca accctgccca gttctcgcgc
     4201 gaaggcatcg tcgccctcgt taacggaggg caggcgcggc ggcgtttccc tggcggcctc
     4261 gggcttcgtg cccgccggaa gtccggtcag cgcctcttcg atggcagaca gagccgcttc
     4321 tgtcggatcg gaggatttct tctgaattgc catgtcaacg cctcgtaacg ctgcgcctct
     4381 gtgcgaggca ggtcgtcaca atgccgtcct ctggcctctg gaccggcatg gtaaacgacc
     4441 tctaaaggcc ttcagtctag ccggtcgaac cttgttggga aacagttggg ttaactcaat
     4501 tgcaaacttc gttaacgatg acggccggtc cggcgcgcaa gaggcaaatc gaccttccat
     4561 tttctgccga tgttaacccg tgatcactag aaggggggac cagaagaagc gcaacagggg
     4621 tccttatgtt ttcttccgat caatccgcca ccacaaaaat gggtggcatc gcctcgtttg
     4681 aacgccccat cgatctcgtg catctggcaa ggcagacgct gggcgacagg gcgctggagc
     4741 aggagatcct ctcgctctac gtgaagcagg cgcgctcgct tctggcacgg atcgagcaat
     4801 gcggctgcgg ccgcgaccgt tccgacctcg cccatacgct gaaggggtcc tcgcgcgccg
     4861 tgggtgcctg gcaggttgcc caggcggcgg aagctgtgga aatcgtgccg ccgggccgcg
     4921 acgccgaatt cgacgcagcg ctcaaggcgc tccacacctg cattgaagcg gcctgcgaca
     4981 cgatcggcga cctgcagcgc gcccactgag gggggcgatt gccccggcag caggcgcgcg
     5041 gacggaaaat ccctctcgcc aaaggcgctt gcagagacta gaggaacgcc gcgcgccctc
     5101 cggggcgcgg cgctcactca tattgccggg attgtccagc catgccgaaa atcacgttcg
     5161 tcgccgccga tggcacgcgt cgcaccgtcg aagccgaaaa cggttcgacc gtcatggagg
     5221 cggcgatcaa gaatggcgtc cccggcatcg aggcggagtg cggcggggcc tgtgcctgcg
     5281 ccacctgtca cgtctatgtc gatgcggagt ggaccgcggt caccggcaag cccgagccga
     5341 ccgaggagga catgctcgac tttgccttcg acatccgtcc aaactcacgt ctgtcgtgcc
     5401 agatcaaggt gagcgacaag ctggatgggc ttgtcgtgca gacgccgacg aaacaggcct
     5461 gaggcgcaac gcccggatcg cctgccacgg cgcgccggtc cgtgcagcgc cggttcaggc
     5521 gcgcaccgaa cccggttgcg ccgcgcctga acccgtccct tcccgaaaga ctgtttcctg
     5581 accctgcctc gggtcgcgcg gaacgatgaa ggacagcacc agcgagatcg tcgcgatcac
     5641 ggcgccggac tggaacacca gggaagggtc cttgatccag agcaggccga agacgaatgg
     5701 aatgaacacc gccgctatgt ggttgatggt gaaggcgact gcagccgtcg gcgccatgtc
     5761 cgccgcatcg ccgatcttct ggaagtaggt cttctgggcg atcatcagcg tcatgatgac
     5821 cccgtcgagg atgaacagaa cggctgcgac gcgaccatca tcgacaatgg cgtagcccgc
     5881 gaacaccagg atcaggcaga cgttctcgat catgatggca tggcgctcgc cgatgcggct
     5941 gatcaacgag ccgaaccacg gggcgagcag catgttcagg caggcggtcg caagcagcag
     6001 cagggcgact tccggcagct tgaaaccaaa cttctcgacc agaaggaacc cggcgaaggc
     6061 gctgaagatc tgccgacggg cgccgctcat cagcgtcagg gcatagtaga gccagtagcg
     6121 cgagcgcaac acgatcgtct tgcgttgtgg cacgctgcct tcgatatggg ggaaccagcg
     6181 ccagaccacg atggtcagaa gtgttcccgc gccgccaagc accgcgaaca ggatggaata
     6241 gtcggtcagc ccgatccacc agagcgcggc catgccagca taggcgatga tcgacgccgc
     6301 cgcgccggca cttgcgacct tgcccatcat ccgggcggcg gcggccttgg gaaacagttg
     6361 cagggccaga gactgattgg ccgtctcgga atagtgaaag ccgatcgaca tgatggtcgt
     6421 ggtgatcacc agcccggtca gcgacggcag gaaccctgtg atcatgattc cgagcgccag
     6481 gatgatcagc gcgacaagag cgaaggtctg ctcccgcatc accgcgaacc aggcaatggc
     6541 cgtgaaggcc aggaagccgg gaatttcgcg cacgctctgc acgatgccga tctccagccc
     6601 gccaaagccg gcagattctt tcgcgaaatt gttgaacagg acgctccagc cggcgaagcc
     6661 gaggaaattg atgaaattga gcgccatcag aaaggcgagc ggcgagcgcg aggtctgcgg
     6721 catggcgaaa aattccggcc gggcgcgacg tgcgctcacg attttcttag aaggcgaagc
     6781 atagcggtcc cggcatgccg gtcaacccgg cgcggcaggc gcgctgtttg tcttgtcgca
     6841 aagcagggcc atattcaacg gcctagagtg acccaatgca aaggaggctc tgatgtacaa
     6901 gaaggttctg gtcccggtcg atctcgccga catggaggtt gccagtccgg cgctccagcg
     6961 tgcgaccgcg ctcgcggcag cttcgggcgg ggagctccgc ctggtcaatg tcctgtcgct
     7021 gatcccggcg gcctatctcg actacatgcc ggcgaacttc gacgtgcagg agcgcgagcg
     7081 cgcccagtcc gacctcaagg cgctcgccgc caagcagggc ttgccggagg gcaaggtgtc
     7141 gagtgtcgtg cggctcggcg cgatctacca cgaagtgctc gccgaggcga aggaatgggg
     7201 tgccgacctg atcgtcacgg gatcgcatga tccgacgttc gcgacctatc tgatcggctc
     7261 gaacgccgcc agcatcgtgc gccatgccca ttgttcggtg ctggtggtgc gcaagaagga
     7321 cgtgtgaggg ccgatcaggc cggccgcggc cggtcctgcg gaagcacggc ttccggaatg
     7381 gccgcggcga caaaccggcg cggccggttg ttgacaacgt agcgccatga ccagaagccg
     7441 aacaggccgg caaaaccgat gatggcggcc gccagcagcc attggccctg cagggcagcg
     7501 cgcccgccca ggaaggcgag cgtgccggtg gtcgccattc ccagcatccc ggacagcgcc
     7561 agcatgaacg gattgcgccc gctgtggagg agcacctgcg gattttccgt cgcggcgcgg
     7621 cggcacagcg ccgtgatgaa tgcgcgatag gcctggccct ggcgctcgac ctgcaccatg
     7681 cccctgaacg tgcgatcctc cagggagatg gtcttgccgt cgcggctgta aatcttggcc
     7741 cggtagcccg acatgtcgat gccgcgcggc gcgaaaaaca gccggatcgt cgcgatggca
     7801 tgatagggat agacgtactg ctggcctgcc tgccaggcga tcagttcctt gcgcccgagt
     7861 tcgaggcgtg tctcggcggc aaacgggcgg gaccggtagg cataggagag cgtcggcgtg
     7921 tccatggcgg gcttcctgcc cgagcgcgcc ggcgttgtcc atggacggaa ccggcaattt
     7981 acggaccttc gcctttttcc tcgattttgt gccggcttga tggcagagat cggccatgaa
     8041 gacactgatc ctgatgcgtc acgccaaggc cgagccgcag cgcggcaagg acgacttcct
     8101 gagagcgctg gccgagcgcg ggcgaacggc agcgccgctg gtcgggggct tcctcaagac
     8161 gaaccggctc gtcgccgaac gtgtccttgt gtccggagcg gtgcgcacgc gggagaccta
     8221 tgcgctggcg gcggccatcg ccggcctgcc gccggccgag ttccagaacg atctgtatct
     8281 ggctccggcg aacgccttgc ttgcctttat ccggaagacg ccggcgcgct cgaagtcgct
     8341 gatgatcatc ggccacaatc cgggtctcgc cgagttgacc cagtggctgg cggcgcccga
     8401 ggaaagcgac ggcgcggcgc tcttcaaggc ggcccgcaag ttccccaccg cctcggtggc
     8461 ggtcttcgat gtgctgacgc cgtggagtga agtcggcgag ggcgattgcg cgctcaagcg
     8521 cttcgtcacg ccttcggatc tgggcggcca cgacgaggac tgacgtgagc cgcgacggtt
     8581 tcggaagctt caccgattcg atgcgcaacg cgtcggcgag cctgttcgac tacctcggca
     8641 gtttcggcca acccacgctg aggcttggcg tcaccggcct ctcgcgctcc ggcaagacgg
     8701 tgttcatcac cgcgctggtg catgcgctca tcagcggcgg gcggctgccg gtgtttcgcg
     8761 cgcgggccga gggcaggctg g
//
LOCUS       DAOMXN010000095         8743 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_4237_length_8743_cov_11.569176, whole genome shotgun sequence.
ACCESSION   DAOMXN010000095 DAOMXN010000000
VERSION     DAOMXN010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 8743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8743
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4237_length_8743_cov_11.569176"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1275)
                     /locus_tag="PK812_08045"
     CDS             complement(<1..1275)
                     /locus_tag="PK812_08045"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HRJ69544.1"
                     /translation="MADHPETGGAGENGALFGSDPAPAASAYRVLARKYRPQTFADLI
                     GQEPMVRTLRNAFAQNRIHQAYIFTGVRGVGKTTTARIIARGLNYETEAGGGPTIDLP
                     GYGKHCQAILESRHVDVIEMDAASHTGIGDIREIIDAVRYRPAYARYKVYIIDEVHML
                     STAAFNGLLKTLEEPPPHVKFLFATTEIRKVPVTVLSRCQRFDLRRIAASEMIAYLRG
                     IAARESAEIEDDALSLIARASEGSMRDALSLMDQAIAHGHGAIGAEELRRMLGLADRA
                     RVIDLFEQLMKGDIAAALTELKNQYDSGADPASIIADIAEYVHLVTRLKIVPGDAGDA
                     ALSETERVRGLAFARTLSQKVLTRAWQILLKGLQEVQAANRPFAAAEMLLVRLAYVAD
                     LPTPDEALRRLKDLQSAHAAAATASGPATGGPS"
     gene            complement(1310..1408)
                     /gene="ffs"
                     /locus_tag="PK812_08050"
     ncRNA           complement(1310..1408)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PK812_08050"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            1497..1928
                     /locus_tag="PK812_08055"
     CDS             1497..1928
                     /locus_tag="PK812_08055"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HRJ69545.1"
                     /translation="MRRFSVHPKIAETTAVVGDLPLSRVLLANERRYPWLMLLPRTEI
                     DEICDLPEADQVQLIRESAAASQAIRTLFNPDKLNIGALGNMVRQLHYHVVGRWTSDA
                     SWPGPVWGKPLDHPYAPEALAAEVARFRQALAPAGLKAAAE"
     gene            1925..2875
                     /gene="nudC"
                     /locus_tag="PK812_08060"
     CDS             1925..2875
                     /gene="nudC"
                     /locus_tag="PK812_08060"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HRJ69546.1"
                     /translation="MNPFFLSESGSPQHLGFAHFEFDRAAHLREDPAGLDALFARPDC
                     RVLALAGEQVLLRNETPLDPLFRTQDLPDDPAWRERAFIGLEHGAPRFAVAYGSQAAE
                     TLGQRTGISLVGLRQLAVETLLSPSALGALACAKALLFWHSRRRFCSVCGQPSKATQG
                     GWRRDCPSCNAQFFPRTDPVVIMLAVRGEHCLMGRQKQFIPGMYSALAGFCEPGETIE
                     DAMRREILEEAGIRVGRVRYLASQPWPFPASLMIGGLAEAVSETILRDETELEDARWF
                     SREEVRQMLRRQHPDNLWVPMKFSLASQLVRAWAEGGAEF"
     gene            2988..4574
                     /locus_tag="PK812_08065"
     CDS             2988..4574
                     /locus_tag="PK812_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014133045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRJ69547.1"
                     /translation="MTTFAALLLAGTMLAGTADARSVKWARSGDALTLDPHSQNEGPT
                     STLGHQIYEPLVTRKVSGELIPTLATSWRILPDDPTTWEFKLRQGVKFHNGNAFNADD
                     VVFSIERAMSPTSDFKGLWASVESVKKADEFTVHIKTKGPNPILIQNMTNTFIMDKEW
                     TEANNAVKVQDFKNKEENFAVRNANGTGAFQLVSREPDVKTVLKRNDNYWGKGEVPLE
                     VTEIVFTPVKADATRIAAMLSGEVDFVQDVPVQDIQRLKSQAGLKVNEGPENRTIFLG
                     MDVAAPELKGSDVKGKNPFADKRVRQAMNMAINRDAIQRVVMRGQSRPTGTIAPPFID
                     GYTPEMGKPPAYDVAKAKAMLAQAGYPNGFSATLNCPNDRYLNDEGICQAAVGMLGQI
                     GIKINMVAQSKTLHFPLIQKNPPETQFFLLGWGVPPFDSDYIFTFLYHTREGARGGWN
                     ALGLSDKALDAKIQSLASQTDLAKRKATVAEIWKELQDQTYYIPLHDQMLAYGMKNDI
                     DIPVHPDNTVWMKYVSFKRS"
     gene            4769..5749
                     /locus_tag="PK812_08070"
     CDS             4769..5749
                     /locus_tag="PK812_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ69548.1"
                     /translation="MPGYILRSLLQGIAVMLVVAALAFAMFRYVGDPIANMVGQEASQ
                     KDRQELAERLGLNDPVPVQFWRFIANAAQGDFGISFRQQRKVSDLLLERLPATMELAL
                     LSAIFATIAGTALGVYTAMRRDGFLSNLLMSLSLVGVSLPTFLIGIGLIYLFAVVLGW
                     LPTFGRGTPTKIGWWTTGLLTLDGWKRLILPTLTLGFFQLTLIMRLVRSQMLEIMSTD
                     YVRFARARGIPEQTIRYRHALKNALVPVVTIAGLQLGSVIAFAIVTETVFQWPGLGAL
                     FVNAVQFADVPVMAAYLMFVAFVFVIVNLIVDLTYVALDPRIALGEKRAR"
     gene            5754..6701
                     /locus_tag="PK812_08075"
     CDS             5754..6701
                     /locus_tag="PK812_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008188696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ69549.1"
                     /translation="MTQTPVPKLTLRQKLDRFLDSDFVHSFKSTPTAVVSALVSLVLI
                     LAAIFAPWITPQNPFNPASLNLMDGLTAPMQPGMGSNRVFWLGADEQGRDVFSALLYG
                     SRISLFVGFASTVFSAAVGVSLGLLAGYFGGKVDALIMRVADVQLSFPAILVALLVFG
                     VARGLIPAQQQEQVSLWVMVVALGLANWAQYARTVRASTLVERQKDYVSAARIIGRAP
                     GPIMGRHVLPNVAGPVLVIATINLALAILEEATLSFLGAGLPVTQPSLGTLIRFGQQQ
                     LFSGEWWILVFPAAMLVALALSINLLGDWLRDALDPKIK"
     gene            complement(6769..7620)
                     /locus_tag="PK812_08080"
     CDS             complement(6769..7620)
                     /locus_tag="PK812_08080"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HRJ69550.1"
                     /translation="MPDSKARTSIAFQGELGANSHILCKETYPDWTALPCATFEDALQ
                     AVKTGKAALGMIPIENSIAGRVADVHHLLPRSGLHIIGEGFLPIRFQLMAPKGARLDG
                     IKDIYSHVHALGQCRKIIRKLGARAHVAGDTAGAAREVAEWGDKSRAALATRLAAEIY
                     GLDILAEDVEDEQNNTTRFVILSKSLVIPPPYEGKTITTFIFRVRNLPAALYKAMGGF
                     ATNGVNMTKLESYMVGGAFTATEFYADVEGHPEETPLKNALEELRFFSKELKILGVYP
                     AAESRGR"
     gene            complement(7639..8370)
                     /locus_tag="PK812_08085"
     CDS             complement(7639..8370)
                     /locus_tag="PK812_08085"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HRJ69551.1"
                     /translation="MTKAVVLIPARMAATRLPGKPLADIHGEPMIVHVWRRGVEAGVG
                     PVVVATDSDAIAAAIRQAGGEAVMTRADHPSGSDRIHEAVERFDPAGTYDIVINVQGD
                     LPTIAPEPVRAALDLLSDSAVDISTIVAEIRREEERAAPSVVKAVGTPVSPNRYRALY
                     FTRATAPTGEGPLFHHIGLYGYRRSALRRFVALSPGWLESRERLEQLRALENGMRIDF
                     SVVDTVPLGVDTPEDLDRARAMLAR"
BASE COUNT         1453 a         2863 c         2874 g         1553 t
ORIGIN      
        1 ggacggtccg cccgtcgcgg gccccgaggc ggtggcggca gcggcatggg cggattgcaa
       61 atccttgagc cggcgcaggg cttcgtccgg ggtcggcagg tcggccacat aggcgaggcg
      121 aaccagcagc atctccgccg ccgcgaaggg acggttcgcc gcctgcacct cctgcaagcc
      181 cttgagcagg atctgccagg cgcgggtgag tactttctgc gacagggtgc gcgcgaaagc
      241 gaggccgcgc acgcgttccg tctcgctgag ggcggcatcg ccggcatcgc ccggcacgat
      301 cttcagccgc gtcacgagat gcacgtattc ggcgatgtca gcgatgatgc tcgcgggatc
      361 ggcgccggaa tcgtactggt tcttcaattc ggtgagggcg gcggcgatgt cgcccttcat
      421 cagctgctcg aacaggtcga tgacgcgggc gcgatcggcc agccccagca tccgccgaag
      481 ctcttccgcg ccgatcgcac cgtgcccgtg cgcgatggcc tgatccatga gcgacagcgc
      541 gtcgcgcatc gaaccctcgg aggcgcgggc gatgagcgag agcgcatcat cctcgatctc
      601 ggcgctctcc cgggcggcga tgccgcgcag ataagcgatc atctcgctgg cggcgatgcg
      661 cctcaggtcg aagcgctggc agcgcgacag caccgttacc ggaaccttgc ggatctcggt
      721 ggtcgcgaac aggaacttca catgcggcgg gggttcctcc agggtcttca agaggccgtt
      781 gaaggcagcc gtcgacagca tgtgcacttc gtcgatgatg tagaccttgt agcgcgcgta
      841 ggccgggcga taacgcacgg cgtcgatgat ctcgcggata tcgccgatgc cggtatggga
      901 ggccgcatcc atctcgatga catcgacatg gcggctttcc aggatcgcct ggcagtgctt
      961 gccgtaaccc ggcaggtcga tggtcgggcc gccgccggcc tcggtctcgt agttcagccc
     1021 gcgcgcgatg atgcgggccg tcgtcgtctt gccgacaccg cgcacgcccg tgaagatata
     1081 cgcctgatgg atgcggttct gcgcaaaggc attgcgcagg gtgcgcacca tcggttcctg
     1141 gccaatgaga tcggcaaaag tctgcggccg gtattttcgc gccagcacgc gataggcgga
     1201 cgccgcaggc gcggggtcac tgccgaaaag cgcaccgttc tcgccggcac cgcctgtttc
     1261 tggatgatcg gccatgcgat gttcagccgg cgctggagcg aaggagaatg ggtgggagac
     1321 cggacaataa cccgcccttg ctcgttaggg ctgcttcctt ccggacctga cccggttggc
     1381 gagtggaacg tccaccgccg gtctcccgcc gcctatatcg ggcaagtgac ccaagaaaac
     1441 aagcttggcg cgcgcgcatg cttttggata atccttgtca gacaacgagg gacatcatgc
     1501 gccgcttttc cgttcatccc aagatcgccg agacaaccgc cgtcgtcggg gacctgccgc
     1561 tgagccgggt cctgctcgcc aacgaacggc gctatccgtg gctgatgctg ctgccgcgca
     1621 ccgagatcga cgaaatctgc gatcttcccg aggccgatca ggtgcagctg atccgggaat
     1681 cggccgccgc gagccaggcc atccgcacgc tgttcaatcc ggacaagctg aacatcgggg
     1741 cgctcggcaa catggtgcgc cagttgcact atcacgtggt cggccgctgg acgagcgacg
     1801 cgagctggcc cgggccggtc tggggcaaac cgctcgatca tccctacgcg cccgaggcgc
     1861 tggcggcgga ggttgcccgg ttccggcagg cgctggcacc ggccggcctg aaggccgccg
     1921 cggaatgaac cccttcttcc tgagcgaaag cggttcgccg cagcatctgg gatttgccca
     1981 tttcgagttc gatcgcgcgg cgcatctgcg cgaggacccg gccgggctcg acgcgctgtt
     2041 cgccagaccc gactgccgtg tcctcgccct cgccggcgaa caggtgctgc tgcggaatga
     2101 aacgccccta gatccgctgt tccggacgca ggacctgccg gatgaccctg cctggcgcga
     2161 gcgggccttc atcggccttg agcacggtgc gccgcgcttc gcggtcgcct atggcagcca
     2221 ggcggcggag acgctcgggc agcgcaccgg catcagcctg gtggggctga ggcaactcgc
     2281 cgtcgagacc ctgctctcgc cctcggcgct gggggcgctg gcttgcgcca aggcgctgct
     2341 gttctggcat tcgcggcgcc ggttctgctc cgtttgcggc cagccgtcga aggcgacaca
     2401 gggcggctgg aggcgcgatt gcccctcctg caacgcccag ttcttcccgc gcaccgatcc
     2461 ggtggtgatc atgctggccg tgcgcggcga gcactgcctg atgggccggc agaagcaatt
     2521 cattccgggc atgtactcgg cgctcgccgg cttctgcgaa ccgggtgaga ccatcgagga
     2581 cgccatgcgt cgcgaaatcc tggaggaggc cggcatacgc gtcgggcggg tgcgctatct
     2641 tgcgtcgcag ccctggccgt ttcccgcctc gttgatgatc ggaggcctgg ccgaggccgt
     2701 gagcgagacg atcctgcggg atgaaacgga actcgaagat gcgcgctggt tctcgcgcga
     2761 ggaggtccgg cagatgctgc ggcgccagca tccggacaac ctctgggtgc cgatgaagtt
     2821 ctcgctggcc tcgcagctgg tgcgggcctg ggccgagggc ggggcggagt tttgaaccgg
     2881 ccggaaagaa tccctcgtga caagggcgga acatcgccct agctttcggc catccgccgg
     2941 tgggaggcgc cggcaccgca gacagggagc tatcgatgaa ataccgcatg acgacttttg
     3001 ccgctctgct tctggccggc accatgctgg ccgggacggc ggatgcgcgc agcgtgaaat
     3061 gggcgcgctc gggcgacgcg ctgacgctcg acccgcattc gcagaacgaa ggcccgacct
     3121 cgacgctcgg ccaccagatc tacgagccgc tggtgacccg caaggtcagc ggcgaactga
     3181 tcccgaccct ggcaacctcc tggcgcatcc tcccggacga tccgacgacc tgggagttca
     3241 agctgcggca gggcgtgaag ttccacaacg gcaatgcctt caacgccgat gacgtagtgt
     3301 tctcgatcga acgcgcgatg tcgccgacct cggacttcaa aggtctgtgg gcctcggtcg
     3361 agtcggtgaa gaaggccgac gaattcacgg tccacatcaa gaccaaggga ccgaacccga
     3421 tcctgatcca gaacatgacg aacacgttca tcatggacaa ggaatggacc gaggcgaaca
     3481 acgccgtgaa ggtccaggat ttcaagaaca aggaagagaa tttcgcggtg cgcaatgcca
     3541 atggcaccgg cgcgttccag ctcgtctcgc gcgagccgga cgtgaaaacc gtgctgaagc
     3601 gcaacgacaa ctactggggc aagggcgagg tcccgctgga ggtgaccgag atcgtgttca
     3661 ccccggtcaa ggccgacgcg acccgcatcg ccgcgatgct ctccggcgag gtcgacttcg
     3721 tccaggacgt gccggtgcag gacatccagc ggctgaagtc gcaggccggc ctgaaggtca
     3781 acgaaggccc cgaaaaccgc accatcttcc tgggcatgga cgtcgccgcg cccgaactta
     3841 agggctcgga cgtgaagggc aagaacccct tcgccgacaa gcgcgtgcgt caggccatga
     3901 acatggcgat caaccgcgat gccatccagc gcgtggtgat gcgtggccag tcgcgcccga
     3961 ccggcacgat cgccccgccg ttcatcgacg gctacacccc cgaaatgggc aagccgccgg
     4021 cctatgacgt ggccaaggcc aaggcgatgc tggcgcaggc cggctatccc aacggcttct
     4081 cggcgactct gaactgcccc aatgaccgct atctcaacga cgaaggcatc tgccaggccg
     4141 ccgtcggcat gctcggacag atcggcatca agatcaacat ggtggcgcag tccaagacac
     4201 tgcacttccc gctgatccag aagaacccgc ccgagacgca gttcttcctg ctcggctggg
     4261 gcgtgccgcc gttcgattcc gattacatct tcaccttcct ctaccacacg cgtgaaggcg
     4321 cgcgcggcgg ctggaacgcc ctcggcctgt cggacaaggc cctggacgcc aagatccagt
     4381 ctctggcctc gcagaccgac ctcgccaagc gcaaggccac ggtcgcggag atctggaagg
     4441 agttgcagga ccagacctac tacatcccgc tgcacgacca gatgctcgcc tatggcatga
     4501 agaacgacat cgacattccg gttcatcccg acaacacggt ctggatgaag tacgtgtcgt
     4561 tcaagcgctc gtaaggacaa ggcgcgaaag gttgggcaac ccgctccagg ctggcgatct
     4621 ggccgatcgc cggcgacggg gcgggcgccc gatccctgac ctgctgccgg atgggaccgg
     4681 gccggatcag gccatgaccc cagcctgacc cgcagccgta cctttccccg caacgggaga
     4741 gggatgtccg caccctttcg gaaccaccat gcccggctat atccttcggt ctctcctgca
     4801 aggcatcgcc gtcatgctgg tcgtggcggc gcttgccttc gccatgttcc gctatgtcgg
     4861 cgacccgatc gccaacatgg tcggacagga ggcgagccag aaggaccggc aggagctggc
     4921 cgagaggctc ggcctcaacg atcccgtccc ggtgcagttc tggcgcttca tcgccaatgc
     4981 ggcgcagggc gatttcggca tctcctttcg ccagcagcgg aaggtttccg acctgctgct
     5041 cgaacgcctc ccggcgacga tggaactggc cctgctgtcc gccatcttcg ccaccatcgc
     5101 cggcaccgcg ttgggcgtct acaccgccat gcggcgcgac ggcttcctgt cgaacctgct
     5161 gatgagcctg tcgcttgtcg gcgtttctct gccgaccttc ctgatcggca tcgggctgat
     5221 ctacctgttc gcggtggtcc ttggctggct gccgaccttc gggcgcggca cgccgacaaa
     5281 gatcggctgg tggaccacgg gcctcttgac cctcgatggc tggaagcgcc tgatcctgcc
     5341 caccctgacg ctcgggttct tccagctgac gctgatcatg cggctggtcc gttcgcagat
     5401 gctggagatc atgagcacgg attacgttcg cttcgcccgc gcccggggca ttccggagca
     5461 gaccatccgc tatcgccacg cgctgaagaa cgcgcttgtt ccggtggtca ccatcgccgg
     5521 gctgcagctc ggcagcgtga tcgccttcgc catcgtcacc gaaacggtgt tccagtggcc
     5581 gggcctcggc gcgctgttcg tcaacgcggt gcagttcgcg gatgtgccgg tgatggcggc
     5641 ctatctgatg ttcgtggcct tcgtcttcgt catcgtgaat ctcatcgtcg atctgaccta
     5701 tgtcgccctc gatccgcgca tcgcgcttgg cgagaagcgg gcacgatagc accatgaccc
     5761 agactcccgt gccgaaactg acgttgcgcc agaaactgga tcggttcctc gattccgatt
     5821 tcgtgcattc cttcaagtcg actccgaccg cggtcgtatc ggctctggtg tcgcttgtgc
     5881 tcatcctggc cgccatattc gcgccctgga tcacgccgca aaatccgttc aatccggcat
     5941 cgctcaacct gatggacggc ctaacggcgc cgatgcagcc gggaatgggc tcgaaccggg
     6001 tgttctggct gggcgccgac gaacaggggc gcgacgtctt ctccgccctg ctctatggca
     6061 gcaggatctc gctgttcgtc ggctttgcct cgacggtgtt ttcggctgcc gtcggcgtga
     6121 gcctcggcct gctcgccggc tatttcggcg gcaaggtgga cgctttgatc atgcgcgtgg
     6181 ccgatgtgca gctgtcgttt cccgcgatcc tcgtggccct gctggtgttc ggcgtggcgc
     6241 gtggcctcat tccggcgcag cagcaggaac aggtgtcgct gtgggtgatg gtggtcgccc
     6301 tcggcctcgc gaattgggcc cagtatgcgc gcacggtccg cgcctccacg ctggtcgaac
     6361 ggcagaagga ttatgtgtcc gcggcgcgca tcatcggccg ggcgccgggg ccgatcatgg
     6421 ggcggcatgt gctgccgaac gtcgccggac cggtcctggt gatcgccacg atcaatctcg
     6481 ccctggccat tctggaggag gcgacgctgt ccttcctcgg tgccggcctg ccggtgaccc
     6541 agccgtcgct cggcacgctg atccgcttcg gccagcagca gctgttctcc ggcgagtggt
     6601 ggattctggt cttcccggcc gcgatgctgg tggcgctggc cctgtcgatc aacctgctcg
     6661 gcgactggct gcgcgacgcg ctcgacccga agatcaagtg aggacgccgg caggattggg
     6721 atgacccggc caaagccggt gtcattcctt tcttccccga gccccagcct atcgaccgcg
     6781 cgattccgcc gccggataga cgccaaggat cttcagttcc ttggaaaaga agcgcagctc
     6841 ctccagcgcg ttcttcaacg gcgtctcttc gggatggccc tcgacatcgg cgtagaattc
     6901 ggtcgcggtg aaggcgccgc cgaccatgta gctttccagc ttggtcatgt tgacgccgtt
     6961 ggtcgcgaag ccgcccatcg ccttgtacag cgccgccggc aggttgcgca cgcggaagat
     7021 gaacgtggtg atggtcttgc cctcataggg cggcgggatc accagcgact tcgacagaat
     7081 gacgaagcgc gtggtgttgt tctgctcgtc ctccacatcc tcggcgagaa tatcgaggcc
     7141 atagatttcg gcggcaaggc gcgtcgccag cgcggcgcgg gatttgtcgc cccattccgc
     7201 cacctcacgc gccgcgccgg cggtatcccc cgccacatgg gccctggcgc ccagcttgcg
     7261 gatgatcttg cggcactggc ccagggcgtg cacgtggctg tagatgtcct tgatgccgtc
     7321 gagccttgcg cccttcggcg ccatcagctg gaagcggatc ggcaggaacc cctcgccgat
     7381 gatgtgcagt ccggacctcg gcaacaggtg atggacgtcc gccacgcggc cggcgatgga
     7441 gttctcgatc gggatcatgc cgagcgcggc cttgccggtc ttcaccgcct gcaaggcatc
     7501 ctcgaaggtg gcgcagggca gcgccgtcca gtccggatag gtctccttgc acaggatatg
     7561 cgaattcgcc ccgagttccc cctggaaggc gatgctggtt ctggctttgc tgtcgggcac
     7621 ggtggctttc cggtcgtgtt agcgggcgag catggcgcgg gcgcggtcga gatcttccgg
     7681 cgtgtccacg ccgagcggca ccgtgtcaac cacggagaaa tcgatgcgca tgccgttctc
     7741 cagcgcgcgc agctgttcca gccgctcgcg gctctccagc caacccgggc tgagcgcgac
     7801 aaatcttcgc agcgccgagc ggcgatagcc gtaaaggccg atgtgatgga acaatggtcc
     7861 ctcgccggtg ggcgcggtgg ccctggtgaa atacagcgcg cgataccggt tcggactgac
     7921 cggcgtgccg acggctttca cgacgctcgg cgccgcgcgc tcctcctcgc gccggatctc
     7981 ggcaacgatg gtggagatgt cgacggcgct gtccgagagc agatcgaggg cggcccgcac
     8041 cggctctggc gcgatggtcg gcagatcacc ctgcacgttg atgacgatgt cgtaggttcc
     8101 ggcaggatcg aagcgttcga ccgcctcgtg gatgcgatcc gaaccggagg ggtggtcggc
     8161 gcgcgtcatc accgcctcgc cgccggcctg ccggatcgcg gcggcaatgg catccgaatc
     8221 ggtcgccacg acaaccggcc cgacaccggc ctcgacgccc cggcgccaga catgcacgat
     8281 catcggttcg ccgtggatgt ccgccaacgg cttgcccggc agacgggtgg cggccatgcg
     8341 ggccggaatg agcacgaccg cctttgtcat ggccgcaatc tccagaagtc ctttggcgct
     8401 ttccacggcc accgcgcgaa aaattgccgg catggcttga aagcgggtca aaaagtagaa
     8461 taggaggggc gcttatacgg gttgcgcgaa gcggtgcaaa gcggctaatc agcaggtggc
     8521 gcacaggagc gcctgtttga ggatttcgcg ccgcgcgggg cgcaggtttt gagcgggagt
     8581 tcggccggct tggccaggct ccgcggaggt cagggcatgg acgcgagtga attcaacaaa
     8641 tacgccggcg cagtgctggg cacgctgacg ttgtcgatgg gtctgtattt cctgtcccac
     8701 ggcctcgttt caccccagaa gcctgccaag ccgggttggg acc
//
LOCUS       DAOMXN010000096         8709 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_4255_length_8709_cov_11.045875, whole genome shotgun sequence.
ACCESSION   DAOMXN010000096 DAOMXN010000000
VERSION     DAOMXN010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 8709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8709
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4255_length_8709_cov_11.045875"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="PK812_08090"
     CDS             complement(<1..238)
                     /locus_tag="PK812_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69552.1"
                     /translation="MNSFFLAPLVIAHRLPQLMFEAFHPNPFAREESARAVTEKVAAL
                     NEGIVAAQAEAMRAPFLIGLAFLNGHSATLAALKT"
     gene            complement(341..1837)
                     /locus_tag="PK812_08095"
     CDS             complement(341..1837)
                     /locus_tag="PK812_08095"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002964909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102662 - malonate-semialdehyde
                     dehydrogenase (acetylating, NAD+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HRJ69553.1"
                     /translation="MIEYGHFIGGKHVVGTSGRTADVWQPMDGTLRGRVALASKAELD
                     AAVENAKAAQPAWAATNPQRRARVLMKFLDLIARDNDKLADCLAREHGKTFPDAKGDI
                     QRGVEVVEVCLGAPHMLKGEFTDGAGPGIDIYSMRQPLGVVCGITPFNFPAMIPLWKL
                     APAIACGNAFILKPSERDPGVPMMLAELALEAGLPPGILNVVNGDKDSVDAILDNRDI
                     QAIGFVGSTPIAEYIYARGCANGKRVQCFGGAKNHMIVMPDADMDQAVDALIGAGYGA
                     AGERCMAISVAVPVGEKTANELVRRLIPRVESLKVGPSTDGAADFGPLVTKAHLEKVK
                     GYVDLGVKEGAKLVVDGRGFKMQGYENGFYMGGCLFDHVTTDMRIYKEEIFGPVLSVV
                     RARDYSEALKMTNDHEYGNGTAIFTRDGDAARDFAARVNVGMVGVNVPIPVPLAYYTF
                     GGWKRSAFGDLNQHGPDAFRFYTKTKTVTARWPSGIKDGADFVIPTMQ"
     gene            1939..2844
                     /locus_tag="PK812_08100"
     CDS             1939..2844
                     /locus_tag="PK812_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRJ69554.1"
                     /translation="MDWDHARIFLATARAGQFLAAARALRLDHATISRRITQLEETLG
                     ARLFERRTNGCVLTPAGERFLAAAERIESDMLRVQAELSDTDVEAAGTVRIGAPDGFG
                     TLFLSGRLGRLMARHPRLTIQLVPLPRAFSLSKREADIAVVIDRPDEGRLHVRKLTDY
                     TLGFYGAPDYLARHGAPANLDDLPRHRLVTYVQDLQFAGALNYFPEGFGPRYRRFECA
                     SVLGQFEAVRSGAGIGILHDYGARGDGGLRRVLSDQTFERTYYLVTHADTRALTRVRA
                     VADFILAEAAAARDDFLGPHKRKGP"
     gene            complement(2956..4230)
                     /gene="aceA"
                     /locus_tag="PK812_08105"
     CDS             complement(2956..4230)
                     /gene="aceA"
                     /locus_tag="PK812_08105"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HRJ69555.1"
                     /translation="MTAPYKPTNKRDFTPADVEKLAGSFKMEFTLAKMGAARLRKLLA
                     ERPFVRTLGALTGNQAMQQVKAGLEAIYLSGWQVAADANNAGQMYPDQSLYPASSVPD
                     VVRRINNTLRRADQIAHMEGDKSTHFMAPIIADAEAGFGGPLNAFELMKAMIEAGAAG
                     VHFEDQLASEKKCGHLGGKVLVPTKTFIRTLNAARLAADVCGVDTVLLARTDAHAANL
                     ITSDVDDYDHPWIDRDSRTDEGFFRLKQGNKHSVEHAIARGLAYGAYADLVWWETSEP
                     DLGEAREFATEFKKKYPDKFLAYNCSPSFNWEKKMSNAEAAKYQEEIAAMGYRYQFVT
                     LAGFHSLNLSMFELAKGYSVRGMGAYSEMQQREFAAQEKGFTAVRHQREVGTGYFDAV
                     AMAISQGKSSTTALEHSTETEQFTAAKKHAAE"
     gene            4438..5865
                     /locus_tag="PK812_08110"
     CDS             4438..5865
                     /locus_tag="PK812_08110"
                     /inference="COORDINATES: protein motif:HMM:NF021382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain fatty acyl-CoA regulator family
                     protein"
                     /protein_id="HRJ69556.1"
                     /translation="MRAVRIGGKLRRLRQEKRLNQAQMAAELSISPSYLNLLESNQRP
                     VTVTVLLKLAERFQVDLASLGGGDDARLTSDLMEALSDPLFEGQDVKASDVRDVVATI
                     PSIAQALVNLYQAYQRGGSAAKAGADDIEGEGQPAALPSEEVTEFLQRRLNYLPELEE
                     AAETLWVDNGLALYTLQQDLVKVLAQKFAVDVEIVAAASMPGVLRSYNPLTRRLALSE
                     MLPASSRTFQLAHQIAFLGYRREVEAAVSGGKFSTPEADALARSALANYFAAAVMMPY
                     ARFLDAARVTRYDLDVLQHRFGVSFEQACHRLTTLRRPGSEAVPFHLIRVDIAGNISK
                     RFSASGIHIARFGAACPRWNVYDAFATPGMLRVQVSRMPDGQTFFCIARTISTAGRSL
                     SRGGLPNRVGQLAIGLGCSSHHAREIVYADGLNLDDPQIVTPIGVSCRTCPRTDCADR
                     AMPSLAQRLEIDENRRGMSTYALAT"
     gene            6002..6988
                     /locus_tag="PK812_08115"
     CDS             6002..6988
                     /locus_tag="PK812_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ69557.1"
                     /translation="MTSGRRLLRVLGRNLLAAVPTVLAIVILNFFLLRLAPGDAADVL
                     AGESGAATAETMAMLRQRFGLDLPVLAQLQTYLGNLAQGSLGFSPRYNMPVMTLILQR
                     LPGTLMLMGMALAVAIALGLAVGTVMALNAGRWPDRLLSVISLLFYSIPGFWIGLMLI
                     VAFSVKLGWLPSGGAETIGANLSGLDYVLDRLRHMLLPAVSLALFYVAVYARLTRAAV
                     LEVAAQDYVRTARAKGLSAFAVGTRHVLRNALVPVTTLAGMHIGGILGGAVVAETVFS
                     WPGLGRLAFESVLRRDYNVLLGVLLLSSILVIVANLIVDLIQAWLDPRIELR"
     gene            6988..7878
                     /locus_tag="PK812_08120"
     CDS             6988..7878
                     /locus_tag="PK812_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ69558.1"
                     /translation="MATLDAAAPSNPAPAPLRRFRALRALLRNPAGVIPLLFLGAVMA
                     MAVLAPRFYPDDPLSMVARPFLWPGVNPAFPLGTDSLGRDVAAGLVWGARISLLVGFS
                     AMLLGVSIGLVVGAVAGYFGGRTDLILVKVIEIFQTLPSFVLLVVLVAIAQPSIGTVT
                     VAIGIVTWPITARLVRAEFRSLREKDFVTAARSLGFGHARIIVREILPNALPPIIVSS
                     SVLVATAILMESALSFLGLSDPNHVSWGSMIGAGRELLRTAWYLSAIPGIAIVLTVLA
                     LNLLGDALNDALNPRLSQER"
     gene            7882..>8709
                     /locus_tag="PK812_08125"
     CDS             7882..>8709
                     /locus_tag="PK812_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ69559.1"
                     /translation="MGAALLSVEDLTIAFPKASPVRSLSFTVEAGETVAIVGESGSGK
                     SLTGLAVMGLLPPGASASGAIRFEGQDVAALAEPALRRIRGARIAMIFQEPMTSLNPV
                     MTIGRQIGEVLRLHEGLSARAARARAIDLLDLVRVSDPHRRVDDYPHRLSGGMRQRVM
                     IAIAVACSPKLLIADEPTTALDVTIQAQVLDLIDRLRREMGMGLVLITHDLGVVGQWS
                     DRVVVMYAGRKVEEAAPDALFANPRHPYTQGLIGASPRSGGTASYRDGPLREIRGGIA
                     "
BASE COUNT         1316 a         2921 c         2901 g         1571 t
ORIGIN      
        1 gcgtcttcag cgccgcaagg gtggcggaat ggccgttgag gaaggcaaga ccgatgagga
       61 agggcgcgcg catcgcctcg gcctgcgccg caacaatgcc ctcgttcagc gccgcgacct
      121 tctcggtcac agcccgcgcg ctttcctccc gggcgaaggg attgggatga aaagcctcga
      181 acatcaactg gggaagccga tgtgcgatga ccagtggcgc gagaaagaac gaattcatgg
      241 cgtcctccga tggagcaagg ccggcaagcg ccccggcgaa cggcggcgct gagcgccgcc
      301 gtccaagcct aggccaggga ccggcccggc gtctcttcga ctattgcatg gtcgggatga
      361 cgaaatccgc gccgtccttg atgccggatg gccagcgggc ggtgacggtc ttcgtcttgg
      421 tgtagaagcg gaaggcatcc ggcccgtgct ggttgaggtc gccgaaggcc gagcgcttcc
      481 agccgccgaa agtgtagtag gccaacggca ccgggatcgg cacgttgacg ccgaccatgc
      541 cgacattgac cctggcggcg aagtcgcggg cggcgtcgcc atcgcgggta aagatcgcgg
      601 tgccgttgcc gtattcgtgg tcgttggtca ttttaagggc ttcggaatag tccctggcgc
      661 gcacaaccga gagcacgggg ccgaagatct cttccttgta gatgcgcatg tcggtcgtca
      721 catggtcaaa caggcagccg cccatgtaga agccgttctc atagccctgc atcttgaacc
      781 cgcgcccgtc gacgaccagc ttggcgcctt ccttcacgcc gagatcgaca tagcctttca
      841 ccttttcgag atgggccttg gtcaccagcg ggccaaaatc cgccgcgccg tcggtcgagg
      901 ggccgacctt gaggctttcg acgcgcggga tgagccgcct gaccagttcg ttcgcagtct
      961 tctcgccgac cggaacggcg accgaaatcg ccatgcagcg ctcgcccgcc gcgccgtagc
     1021 ccgcgccgat cagcgcatcg accgcctggt ccatgtcggc gtccggcatg acgatcatgt
     1081 ggttcttggc gccgccgaaa cactggacgc gcttaccgtt ggcgcaaccg cgcgcgtaaa
     1141 tgtactcggc aatcggcgtc gagccgacga atccgatggc ctgaatgtcg cggttgtcga
     1201 gaatcgcatc gaccgagtcc ttgtcgccgt tgacgacatt gaggatgccg ggcggcaggc
     1261 cggcctccag cgccagttcc gccagcatca tcggcacgcc ggggtcgcgc tcggacggct
     1321 tcaggatgaa ggcgttgccg caggcgatgg ccggggcgag cttccacaac gggatcatcg
     1381 ccgggaagtt gaacggcgtg atgccgcaga cgacgccgag cggctggcgc atggaataga
     1441 tgtcgatgcc cgggccggcg ccgtcggtga actcgccctt cagcatgtgc ggcgcaccga
     1501 gacagacctc gaccacctcg acgccgcgct ggatgtcgcc cttggcgtcg gggaaggtct
     1561 tgccatgctc gcgcgccagg cagtccgcca gcttgtcgtt gtcacgggcg atcagatcga
     1621 ggaacttcat caggacgcgc gcgcgccgct gcggattggt cgcggcccag gccggctgcg
     1681 ccgccttggc gttctccacc gccgcgtcca gctccgcctt ggaggcgagc gcgacgcgcc
     1741 cgcgcagcgt gccgtccatc ggctgccaaa catctgccgt gcggcccgat gtgccgacga
     1801 catgcttgcc gccgatgaaa tgtccgtact cgatcatgga tgcctccgaa tgttccctcg
     1861 caggcgtcat aggacttgcg caggctcaca gcaacgcgga agatcgcgca tccgttgtgc
     1921 agcaatgcga gtgccggcat ggattgggat cacgcccgca tctttctggc cacggcgcgc
     1981 gccggccagt tcctcgcggc ggcgcgggcg ctgcggctcg atcacgccac catcagccgg
     2041 cgcatcactc aactggagga aacgctcggc gccaggcttt tcgagcggcg caccaacggt
     2101 tgcgtgctga cgccggcggg cgagcgcttc cttgccgccg ccgagcgcat cgagtccgac
     2161 atgctgcggg tgcaggccga actttccgat accgatgtgg aagcggcggg caccgtgcgc
     2221 atcggcgcgc cggacgggtt cggcacgctg tttctctccg gccggctcgg acggctgatg
     2281 gcgcggcatc cgcgcctgac catccagctt gtgccgctgc cgcgcgcctt ctcgctgtcg
     2341 aaacgcgagg ccgacatcgc ggtggtgatc gaccggccgg acgaggggcg tctgcatgtg
     2401 cgcaagctga cggactatac gctcggcttc tacggcgcac ccgattacct ggcgcggcac
     2461 ggggcgcccg cgaacctcga tgacctgccg cgccaccggc tggtgaccta tgtgcaggac
     2521 ctgcaattcg ccggcgcgct caactacttt ccggaagggt ttggtccgcg ctaccggcgt
     2581 ttcgagtgcg ccagcgtgct cggccagttc gaggccgtgc gctccggcgc ggggatcggc
     2641 atcctgcacg attacggggc gcgcggcgat ggtggcctgc gccgggtcct gtccgaccag
     2701 acgttcgagc ggacctacta tctcgtcacg catgcggaca cgcgcgcgct gacgcgggtg
     2761 cgcgcggtcg cggattttat cttggcagaa gccgcggcgg cgcgggacga cttcctcgga
     2821 ccgcacaaaa gaaaagggcc gtgaccccag gggccacggc cttccaagtg gaccggcaat
     2881 tccgaaaaat cggcgcttgc ggtcggggga gatcctggcc gggcggtgcc cggccttaac
     2941 gcgtcgaacg ctggcttact cagccgcgtg cttcttggcg gccgtgaact gctcagtctc
     3001 ggtcgagtgc tccagagcgg tcgtggagga cttgccctgc gagatcgcca tggcgaccgc
     3061 gtcgaagtag ccggtgccga cctcgcgctg gtggcgaacg gcggtgaagc ccttctcctg
     3121 agccgcgaac tcgcgctgct gcatctcgga ataggcgccc atgccgcgca ccgaatagcc
     3181 cttggccagc tcgaacatcg acaggttgag cgagtggaag ccggccagcg tgacgaactg
     3241 gtagcggtag cccatcgcgg cgatttcttc ctggtacttc gccgcctcgg cgttgctcat
     3301 cttcttctcc cagttgaagg aaggcgagca gttgtaggcg aggaacttgt ccggatactt
     3361 tttcttgaac tcggtggcga actcgcgggc ttcgccgagg tccggctcgg aggtttccca
     3421 ccagacgaga tcggcgtagg cgccgtaggc gaggccgcgg gcgatagcgt gttcgaccga
     3481 gtgcttgttg ccctgcttca ggcggaagaa gccctcgtcg gtgcggctgt cgcgatcgat
     3541 ccagggatga tcgtagtcgt ccacatccga ggtgatgaga ttggcagcgt gcgcgtcggt
     3601 gcgggcaagc agcaccgtgt ccacgccgca gacgtcggcc gcgaggcgcg ccgcgttcag
     3661 cgtgcggatg aaggtcttgg tcgggaccag caccttgccg ccgagatggc cgcacttctt
     3721 ctcggaggcc agctgatcct cgaagtggac gccggccgcg cccgcttcga tcatcgcctt
     3781 catcagttcg aaggcgttca gcggaccgcc gaagccggcc tcggcgtcag cgatgatcgg
     3841 cgccatgaaa tgcgtcgact tgtcgccttc catgtgggcg atctggtcgg cgcggcgcag
     3901 ggtgttgttg atgcggcgca ccacgtccgg cacggacgag gccggataga gcgactggtc
     3961 gggatacatc tggccggcgt tgttggcgtc agcggcgacc tgccagcccg acagatagat
     4021 ggcctcgaga ccggccttca cctgctgcat ggcctgattg ccggtcagcg cgccgagggt
     4081 gcgcacgaac ggccgctccg cgagcagctt gcgcagacgg gccgcgccca tcttcgccag
     4141 cgtgaattcc atcttgaacg aaccggcgag cttctcgaca tcggcaggcg tgaaatcgcg
     4201 cttgttcgtc ggcttgtagg gagcggtcat cgatcaacct ctgttggaat gctgtctctc
     4261 cggaggggca cgcgccgcgc tccgtggatg ggctttacat gtcacaggtt aagtgtgcat
     4321 cgcaataaga cgttgaaatg agacgaaaaa tgcggaaaag cctcgacatc ttgacaattt
     4381 ccattttgtg aattgtaatg tttccgtaaa ttctgctgca aaatcaagga cccgaccatg
     4441 cgtgcggtgc ggatcggcgg caagctcagg cgtctgcgcc aggaaaagcg ccttaaccaa
     4501 gcgcagatgg cggccgaact cagcatctcg ccaagctacc tcaacctgct ggagagcaac
     4561 cagcgtccgg tgacggtcac cgtactactg aagctcgccg agcgattcca ggtggacctt
     4621 gccagcctcg gcggcggcga cgacgcgcgc ctgacctccg acctgatgga ggccctgtcc
     4681 gatccgctgt tcgaggggca ggacgtgaag gcctccgacg tgcgcgacgt ggtggcgacg
     4741 ataccttcca tcgcccaggc actggtcaac ctctaccagg cgtaccagcg cggcggctcc
     4801 gcggcgaagg ccggggccga cgacatcgag ggcgagggcc agccggcggc gctgccgtcc
     4861 gaagaggtca cggaattcct ccagaggcgg ctgaactacc tgcccgagct ggaagaggca
     4921 gccgagacgc tgtgggtcga caatggcctg gcgctctaca cgctccagca ggacctggtg
     4981 aaggtcctgg cgcagaagtt cgccgtggac gtggagatcg tggcggcggc gtcgatgccc
     5041 ggggtcctgc gcagctacaa ccccctcacc cgccgtctgg cgctgtccga gatgctgccg
     5101 gcgtcgagcc gcaccttcca gctcgcgcat cagatcgcct ttctcggcta ccggcgcgag
     5161 gtcgaggccg cggtctccgg tggcaagttc tcgacgccgg aagcggatgc gctggcccgc
     5221 tcggcgctcg ccaactactt cgccgccgcg gtgatgatgc cctacgcccg ctttctcgat
     5281 gcggcgcgcg tcacccgcta cgatcttgac gtgttgcagc atcggttcgg cgtttccttc
     5341 gagcaggcct gccaccggct caccacgctg cgccggcccg gttccgaggc ggtgccgttc
     5401 cacctcatcc gtgtcgacat cgccgggaac atctccaagc gcttctccgc ctccggcatc
     5461 catatcgccc gattcggcgc cgcctgcccg cgctggaacg tctatgacgc cttcgccacg
     5521 cccggcatgc tgcgcgtgca ggtctcgcgc atgcccgacg gccagacgtt cttctgcatc
     5581 gcccgcacca tctccaccgc cgggcgctct ctctcgcggg gagggctgcc gaaccgcgtc
     5641 gggcagctgg ccatcggcct gggatgctcg tcacaccatg cgcgcgagat cgtctatgcc
     5701 gacggcctca acctcgacga tccgcagatc gtcacgccca tcggcgtctc ttgccgcacc
     5761 tgtccgcgca cggattgcgc cgaccgcgcc atgccgtcgc tggcgcagcg cctagagatc
     5821 gacgagaatc ggcgcggcat gagcacttac gcgctggcga catgacgcca catcatcagg
     5881 caggcaagca gcggacgcga tcttcggtcg tcggtgcccc cttgcatcgc aggcgcaagg
     5941 ctcactacaa ccgtgacggg actggtggcc cgcgtcgggc cgaacctact gggatgctag
     6001 catgacctcc ggccggcggc tgctgcgcgt gctcggccgc aaccttctgg cggccgttcc
     6061 gaccgtgctc gccatcgtca tcctcaattt cttcctgctg cggctcgcgc ccggcgatgc
     6121 ggcagacgtg ctggcgggcg agtctggcgc ggcgacggcg gagacaatgg cgatgctgcg
     6181 ccagcgcttc ggcctcgatc ttccggtgct ggcccaactc cagacctacc tgggcaacct
     6241 cgcgcagggg agcctcggct tttcgccgcg ctacaacatg ccggtcatga ccctcatcct
     6301 ccagcgcctg cccggcacgc tgatgctgat gggcatggcg ctggcggtcg cgatcgcgct
     6361 cggcctggcg gtcggcacgg tgatggcgct gaacgccgga cgctggccgg accggctgct
     6421 ttcggtcatc tcgctgctgt tctattccat ccccggcttc tggatcggcc tgatgctcat
     6481 cgtggcgttc tcggtaaaac tgggctggtt gccgagcggg ggcgcggaaa ccatcggcgc
     6541 caatctgtcc ggcctcgact acgtgctcga ccgactgcgc cacatgctgc tgccggcggt
     6601 ctcgctggcg ctgttctatg tcgcggtcta tgcgcggctg acgcgggcgg ccgtgctgga
     6661 ggtggcggcg caggactatg tgcgcaccgc gcgcgccaag ggcctgtccg ccttcgccgt
     6721 gggcacgcgg cacgtgctgc gcaacgccct cgtcccggtc accacgcttg ccggcatgca
     6781 tattggcggc atcctcggcg gcgccgtggt ggcggaaacg gttttctcct ggccgggcct
     6841 cggccgcctt gccttcgagt cggtacttcg acgcgactac aacgtgctgc tcggcgtgct
     6901 gctcctgtcc tcgattctcg tcatcgtggc gaatctcatc gtggacctca tccaggcgtg
     6961 gctggacccg cgcatcgagt tgcgctgatg gcgacgctcg atgcggctgc cccttcgaat
     7021 ccggcacccg cgccgctgcg gcgcttccgc gccctgcgcg cgttgctgcg caatcccgcc
     7081 ggcgtgatcc cgctgctgtt cctgggcgcg gtgatggcga tggctgtcct ggcaccccgg
     7141 ttctatccgg acgatccgct gtcgatggtc gctcgcccgt tcctgtggcc cggcgtcaat
     7201 cccgcattcc cgcttggcac ggattcgctg ggccgcgacg tggcggcggg gctggtctgg
     7261 ggggcgcgca tctcgctgct ggtcggtttc tcggccatgc tgcttggcgt cagcatcggg
     7321 ctcgtcgttg gtgccgtggc cggctacttc ggcgggcgga cggacctgat tttggtcaag
     7381 gtcatcgaga ttttccagac gctgccaagc ttcgtgctgc tggtggtgct ggttgccatc
     7441 gcccagccca gcatcggcac ggtcaccgtc gccatcggca tcgtcacctg gccaatcacg
     7501 gcgcggctgg tgcgcgccga attccgctcg ctgcgcgaga aagacttcgt cacggcggcg
     7561 cgcagcctcg gatttggcca tgcccgcatc atcgtgcgcg agatcctgcc caatgccttg
     7621 ccgccgatca tcgtctcttc ctcggtgctg gtggccaccg cgatcctgat ggaatcggcg
     7681 ctgtcgttcc ttggtctcag cgatcccaac catgtcagct ggggatcgat gatcggcgca
     7741 ggccgcgaac tgctgcgcac ggcctggtac ctcagcgcga ttccgggcat cgccatcgtg
     7801 ctgacagtgc tggccctcaa cctcctgggc gatgcgctga acgatgcgct caaccctcgc
     7861 ctgtcgcagg agcgctaggc catgggcgcc gcgttgcttt ccgtcgagga cctgaccatc
     7921 gcctttccca aggccagtcc ggtgcgatcg ctgtcgttca ccgtcgaggc gggcgagacc
     7981 gtcgccatcg tcggggaatc ggggtcaggc aagtcgctga ccgggcttgc cgtcatgggg
     8041 ttgctgccgc cgggcgccag cgcgagcggt gcgatccgct tcgaaggcca ggacgtggcc
     8101 gcgctggcgg agccggcgct gcgccggatt cgcggcgcca ggatcgccat gatcttccag
     8161 gagccgatga cctcgctcaa tccggtgatg accatcggtc gacagatcgg cgaggtcctg
     8221 cgcctgcatg aaggcctgtc ggcccgcgcc gcccgtgccc gcgccatcga cctgcttgac
     8281 ctggtgcgcg tttccgatcc gcaccggcgt gtcgacgact atccgcatcg gctctccggc
     8341 ggcatgcgcc agcgcgtgat gattgccatc gccgtggcct gcagcccgaa actgctgatt
     8401 gcggacgagc cgaccaccgc cctcgacgtc accattcagg cgcaggtgct cgatctcatc
     8461 gaccggctgc ggcgcgagat gggcatgggg ctggtcctga tcacacacga cctcggcgtc
     8521 gtcggacaat ggtccgaccg cgtggtggtg atgtatgccg ggcgcaaggt cgaggaggcc
     8581 gcgccggacg cgctcttcgc gaacccgcgc cacccttaca cccaggggtt gatcggcgcc
     8641 tcgccccggt ccggcggaac ggcgagctac cgcgacgggc cgttgcggga gattcgcggc
     8701 ggcatcgcc
//
LOCUS       DAOMXN010000097         8698 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_4263_length_8698_cov_13.706930, whole genome shotgun sequence.
ACCESSION   DAOMXN010000097 DAOMXN010000000
VERSION     DAOMXN010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 8698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8698
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4263_length_8698_cov_13.706930"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="PK812_08130"
     CDS             complement(<1..604)
                     /locus_tag="PK812_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRJ69560.1"
                     /translation="MADVTLSGIRKIYPGGFEAVKGVSFAIPDGGFCVLVGPSGCGKS
                     TLLRMIAGLETITHGEAKIGGRVVNRIEPAERDIAMVFQNYALYPHMSVYDNMAYGLR
                     NRKTPEDEIKRRVADAARILAIEPLLERKPRALSGGQRQRVAMGRAIVRKPQVFLFDE
                     PLSNLDAKLRVHMRVEIKKLHNALKVTSIYVTHDQVEAMTL"
     gene            complement(623..1474)
                     /gene="ugpE"
                     /locus_tag="PK812_08135"
     CDS             complement(623..1474)
                     /gene="ugpE"
                     /locus_tag="PK812_08135"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HRJ69561.1"
                     /translation="MVERETLLSRILPHAILMLGVLVVFFPVYLAFVASTWDPATISN
                     GKMGILPGPYMIENYQRILFTGTSSSTREPVFNMMVNSFIMAVMIAVGKIFISILSAY
                     AIVYYRFPFRMAAFWIIFITLMLPVEVRIYPTYKIVSDLKLLDTYSGLTIPLIASATG
                     TLLFRQFFMTIPDELLEASKIDAAGPFRFFKDTVLPLSMTTIAALFVIQFIYGWNQYL
                     WPLLITTKDSMATIVIGIKKMIVTQDALTEWQLAMATAVLALLPPVAVVVGMQKLFVK
                     GLVETEK"
     gene            complement(1474..2355)
                     /gene="ugpA"
                     /locus_tag="PK812_08140"
     CDS             complement(1474..2355)
                     /gene="ugpA"
                     /locus_tag="PK812_08140"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HRJ69562.1"
                     /translation="MEKRTVFSSYAIPAALLLPQLLITIVFFYLPAGQAVWQSFQLED
                     AFGTSTQFVGFENYQILFSKPEYFKAMGVTLIFSSAVAFMSLSISLLLAVMANKHIAG
                     AGAYKTLLIWPYAVAPAVAGVLWLFMFQPSLGMVSGVLKTWGIDWNPLLNSNHAMILL
                     VMAATWKQISYNFLFFLAGLQSIPHSLIEAASIDGARPARRFWSIVFPLLSPTTFFLL
                     VVNITYVFFDTFGIIDAVTGGGPAGSTTTLVFKVYHDGKSGGDLGLSAAQSVILMILV
                     VGLTAIQFKYIERKVSY"
     gene            complement(2441..3763)
                     /gene="ugpB"
                     /locus_tag="PK812_08145"
     CDS             complement(2441..3763)
                     /gene="ugpB"
                     /locus_tag="PK812_08145"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HRJ69563.1"
                     /translation="MSIFSKTALMAATALTLAASPALAQVEIQWWHAMTGANNDVVNK
                     LAADFNASQNEYKVVATYKGSYADTMNAGIAAFRAGNAPHIMQVFEVGTASMMAAGKA
                     IKPVHELMKEAGEKFDPAAYLPSVAGYYSTVKGEMLSFPFNSSSTVMWYNKDAFKKAG
                     LNPDAPPKTWPEVFDHAKKLQGAGYASCGFSTAWITWVNIEQFSVWHNIPIGTKANGI
                     DGFDTEFKINSPVHVKHWANLAELQKTKTFDYSGRTNTGEGRFTSGECPIFLTSSAFL
                     GNVKANAKFDWANAPMPYYPDVAGAPQNSTLGGASLWVMGGKAANDYKGVAKFFTFLS
                     DTDRQAKLHTDSGYLPITTAAYNKVKASGYYKDNPYNETPLKELTNKPPTENSRGLRF
                     GNLVQIRDIWAEEIEEILTGKKTPQAGADAMVSRGNAVLRQFERTVSR"
     gene            complement(3928..5466)
                     /locus_tag="PK812_08150"
     CDS             complement(3928..5466)
                     /locus_tag="PK812_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018263226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HRJ69564.1"
                     /translation="MNAPVSPRTQPRVLKLHDTDSVLVAVDAIEPGVAAQGITAKERI
                     PRGHKMARVALAEREPVRKFGQIIGFAARAITPGEWIHEHNCTFAAFDRDYAFCADAR
                     DDGLLPEAQRATFEGFRRANGRVGTRNYIGILTSVNCSATVAKFMAEEINRSGILADY
                     PNVDGVIPLVHGGGCALDIKGEGYEVLKRTQWGYAANPNMAGVVMVGLGCEGFQIARW
                     KEAYGMSESDTFRTMTIQEVGGTKKTVAAGVEAIKAMLPIANRAVRSTQPASELMLAL
                     QCGGSDGYSGITANPALGVAADLLVKHGGTAILSETPEIYGAEHLLTRRAATREIGEK
                     LVAIIRWWEDYTARNKMDMNNNPSPGNKAGGLTTILEKSLGAAAKGGNSTLSGVYHYA
                     EPVTAKGFVYMDTPGYDPVGVTGQVAGGANVLCFTTGRGSAFGCKPTPSIKLATNSMI
                     YEKMIDDMDINCGDVLDGVTLEDKGREIFEVILRVASGERSKSEQLGYGDAEFVPWTI
                     GATM"
     gene            5565..6740
                     /locus_tag="PK812_08155"
     CDS             5565..6740
                     /locus_tag="PK812_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRJ69565.1"
                     /translation="MRIDLTPENTLPHDGTSGALAGRVWRPEHKGPSVAAIRANGVFD
                     ISRSFPTMRDLCETADPARALDDAAGERIGDLAAILSNTPEDSRDPSKPWLLAPIDLQ
                     AVKAAGVTFAVSMLERVIEERARGNPAAAAEIRKEVTRLVGDDLSKLKPGSPEAMRLK
                     DVLIAQGVWSQYLEVGIGPDAEIFTKAQVLSAVGTGADAGLHPISTWNNPEPEVVIVV
                     ASDGRIVGATLGNDVNLRDVEGRSALLLSKAKDNNASAAVGPFLRFFNATFSLDDVRR
                     TEVALRVEGQDGFLLEGASNVAKISRDPADLARQLLGRHHQYPDGAVLYLGTMFAPVK
                     DRDGAGQGFTHKLGDVVTIAAPKLGRLVNRMTHSDKAAPWTFGASHLMRNLAKRGLL"
     gene            complement(6789..7997)
                     /gene="pcaF"
                     /locus_tag="PK812_08160"
     CDS             complement(6789..7997)
                     /gene="pcaF"
                     /locus_tag="PK812_08160"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HRJ69566.1"
                     /translation="MSRDVYICDYVRTAIGRYSGSLAKVRADDLAAIPVRELAKRHPG
                     LKDAVQEIFFGCANQAGEDNRNVGRMAALLAGLPETVPAVTLNRLCASGMDAVGQGAR
                     AIRAGEVDIVLAGGVESMTRAPFVMGKAETAFARSADIFDTTIGWRFINPLMKKQYGV
                     DSMPETAENVAADHQISRADQDAFALRSQQRAARAQAAGYFDGEIVPVEIKDAKGNVT
                     VFAKDEHGRADTTAEMLAKLRPIVKPEGTVTAGNASGVNDGAAAMILASADAARKHGL
                     KPIAKVLGFAAAGVAPRVMGIGPVPASRLLADRLGLKMSDFDVIELNEAFASQGLATL
                     RQLGVADDGEHVNPNGGAIALGHPLGMSGTRITGTAARELQRRGGRYALATMCVGVGQ
                     GAAIALERVG"
     gene            complement(8071..>8698)
                     /locus_tag="PK812_08165"
     CDS             complement(8071..>8698)
                     /locus_tag="PK812_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69567.1"
                     /translation="FLRRWQSERLARTHADLLADARHHDAARFFLDEIYGLKDTAQRD
                     ADVARVAPTLTRLLPEGGLETVADAVELDALSEDLDAAMVKALGRKVRALDAVVYGNA
                     YRKVGRKDDRLRQIGLVDHLGHALDRLTRRAFAGTALRMMRKPAELAGLAELQAFLQS
                     GYDAFRKMGRADAFLARIIRRESAILEALFSGDDRLLDAGPEAFGGTG"
BASE COUNT         1512 a         2780 c         2850 g         1556 t
ORIGIN      
        1 cgagggtcat cgcctcgacc tgatcgtgcg tgacgtagat cgaggtgacc ttgagcgcat
       61 tgtggagctt cttgatctcg acgcgcatgt gcacgcgcag cttggcatcg agattggaga
      121 gcggttcgtc gaacaggaac acctgcggct tgcgtacgat ggcgcggccc atggcgacgc
      181 gctggcgctg tccgccggag agcgcgcggg gcttgcgttc cagcagcggc tcgatggcga
      241 gaatccgagc ggcatccgcc acgcggcgct tgatctcgtc ctccggagtc ttccggttcc
      301 tcaggccata ggccatgttg tcgtagacgc tcatgtgcgg gtagagcgca tagttctgga
      361 acaccatggc gatgtcacgc tcggccggct cgatgcgatt gacgacccgc ccgccgatct
      421 tcgcctcgcc gtgcgtgatc gtttcgagac ccgcgatcat gcgcagcagc gtcgatttcc
      481 cgcagccgga cggaccgacc agcacgcaga agccgccgtc tggaatggcg aaggaaacgc
      541 ccttcacggc ctcgaaaccg ccgggataga tttttcggat gcccgaaagg gtgacgtcag
      601 ccatgcgatg ctcccgactg cctcacttct ccgtctccac cagccccttg acgaagagct
      661 tctgcatgcc caccacgacc gcgaccggcg gcagcagggc aagcacggcg gtggccatgg
      721 cgagctgcca ttcggtcagc gcgtcctgcg tcacgatcat cttcttgatg ccgatgacga
      781 tggtcgccat ggaatccttg gtggtgatca gcagcggcca gagatactgg ttccagccat
      841 agatgaactg gatgacgaac agcgccgcga tggtggtcat cgacagcggc agcaccgtgt
      901 ccttgaagaa acggaacggc ccggccgcat cgatcttcga ggcctccaag agttcatccg
      961 gaatggtcat gaagaactgg cggaacagca gcgttccggt cgccgaggcg atcagcggaa
     1021 tggtgaggcc ggaataggta tcaagcagct tgaggtccga gacgatcttg taggtcgggt
     1081 agatgcgcac ctccacaggc agcatcagcg tgatgaagat gatccagaac gccgccatgc
     1141 ggaacggaaa acgataatag acgatggcgt aggccgaaag gatcgagatg aaaatcttcc
     1201 ccaccgcgat catcaccgcc atgatgaagg aattcaccat catgttgaac accggctcgc
     1261 gcgtcgagga cgaggtgccg gtgaacagga tccgctgata gttctcgatc atgtaggggc
     1321 cgggcaatat ccccatcttg ccgttgctga tcgtcgccgg gtcccaggtc gaggccacga
     1381 aggcaaggta gaccgggaaa aacaccacca gcacgcccag catcaggatt gcgtggggca
     1441 gaatgcgtga cagcagggtc tccctctcga ccatcagtag ctgaccttgc gctcgatgta
     1501 cttgaactgg atcgccgtca ggccaaccac caggatcatc aggatcacgg actgtgccgc
     1561 ggaaagcccg agatcgccgc ccgacttgcc gtcatggtag accttgaaca cgagcgtcgt
     1621 ggtcgaacct gccgggccgc cgccggtcac cgcgtcgatg atgccgaacg tgtcgaagaa
     1681 gacgtaggtg atgttgacga ccagaaggaa gaacgtggtc ggcgacagca gcgggaacac
     1741 gatcgaccag aaccgtcgcg ccggacgggc gccatcgatg gaggcggcct cgatcaggct
     1801 gtgcgggatc gattgcagtc cggccaggaa gaacaggaaa ttgtaggaga tctgcttcca
     1861 cgtcgccgcc atcaccagca ggatcatggc atggttgctg ttcagcaggg gattccagtc
     1921 gatcccccag gtcttcagga cgcccgagac catgcccagt gagggctgga acatgaacag
     1981 ccagagcaca ccggcgacgg cgggcgccac cgcatagggc cagatcaaca gcgtcttgta
     2041 ggcgccggct ccggcgatgt gcttgttcgc catcaccgcc agcagcagcg aaatcgacag
     2101 cgacatgaaa gcaacggcgc tggagaagat cagcgtcacg cccatggcct tgaagtattc
     2161 cggcttggaa aaaaggatct ggtagttctc gaagccgacg aattgggtcg aggtgccgaa
     2221 ggcatcctca agctggaagg actgccacac cgcctgcccg gccggcaggt agaaaaagac
     2281 gatcgtgatc aagagttgcg gcagcagcag cgccgccggg atcgcgtaac tggagaagac
     2341 ggtgcgcttt tccatgatac ggcggacggc cctcccgatt gtgctgtcgg ccggcgcggt
     2401 tcagggcgcc agccgacagt tcagggacga ccgccttcct ttagcgcgac acggtgcgct
     2461 cgaactggcg cagcacggcg ttgccgcggc tgaccatggc atcagcgccg gcctgcggcg
     2521 tcttcttgcc ggtgaggatt tcctcgatct cttccgccca gatgtcgcgg atctgcacga
     2581 ggttgccaaa gcgcaggccg cgtgaattct cggtcggcgg cttgttggtg agctccttca
     2641 ggggcgtctc gttgtacgga ttgtccttgt aatagcccga agccttgacc ttgttgtagg
     2701 cagccgtggt gatcggcagg tagccggaat cggtgtgcag cttggcctgc ctgtctgtat
     2761 cggacaggaa ggtgaagaac ttcgccacgc ccttgtagtc gttggcggcc ttgccgccca
     2821 tgacccagag cgaggcgccg cccagcgtcg agttctgcgg cgcgccggcg acgtcgggat
     2881 agtacggcat cggcgcattg gcccagtcga acttggcgtt ggccttgacg ttgccaagga
     2941 atgccgagga cgtcaggaag atcgggcatt cgccggaggt gaagcgacct tcaccggtgt
     3001 tggtgcgacc ggaatagtcg aaggtcttgg tcttctgcag ctcggccagg ttggcccagt
     3061 gcttcacatg caccggcgag ttgatcttga actcggtgtc gaagccgtcg atgccgttgg
     3121 ccttggtgcc gatcgggatg ttgtgccaga ccgagaactg ctcgatgttc acccaggtga
     3181 tccaggccgt cgagaagccg caggaggcat agcccgcgcc ctgcagtttc ttggcgtgat
     3241 cgaacacttc cggccaggtc ttcggcggcg cgtcgggatt caggccggcc ttcttgaagg
     3301 cgtccttgtt gtaccacatc accgtggagg aggagttgaa cgggaaggac agcatctcgc
     3361 ccttcacggt ggaataataa cctgcgacgg aaggcaggta ggcggccgga tcgaacttct
     3421 cgcccgcctc tttcatcagt tcatgcacgg gcttgatcgc cttgccggcg gccatcatcg
     3481 atgcggtgcc gacctcgaac acctgcatga tgtgcggcgc attgccggcg cggaaggccg
     3541 cgatgccggc gttcatggtg tcggcgtagc tgcccttgta ggtcgcgacg accttatact
     3601 cgttctgcga cgcattgaaa tcggcggcca gcttgttgac cacatcgttg ttggcaccgg
     3661 tcatcgcgtg ccaccactgg atttccacct gagccaacgc gggcgacgcg gccagcgtca
     3721 gcgcggtggc cgccatcaag gctgtcttgg aaaatatcga cattcttctg ctccctgttt
     3781 cgcccgacct cccgggcggc atctctggtc tatcccctgc gccttacgcc agcgccatga
     3841 caacgggatg acctgtatga caggcagtca atcacgccgg acaacgctcg gcaatgggcc
     3901 ggatgtgact tttcgtggct tttcggttca catcgtcgcg ccgatggtcc agggcacgaa
     3961 ttcggcgtcg ccatagccca gctgctcgga cttcgagcgc tcgcccgagg cgacacgcag
     4021 gatcacctcg aaaatctcgc gacccttgtc ctccagcgtc accccgtcca gcacatcgcc
     4081 gcagttgatg tccatgtcgt cgatcatctt ctcgtagatc atcgaattgg tggcaagttt
     4141 gatcgaaggc gtcggcttgc agccgaaggc agagccgcga ccggtggtga agcacaggac
     4201 gttggccccg cccgcgacct ggccggtgac gccgaccgga tcgtagcccg gcgtatccat
     4261 gtagacgaag cccttggcgg tcaccggctc ggcatagtga tagacgccgc tgagggtcga
     4321 attgccgccc ttggccgccg cgccaaggga tttttcgagg atcgtcgtca gtccgccggc
     4381 cttgttgccg ggagacgggt tgttgttcat gtccatcttg ttgcgggcgg tgtagtcttc
     4441 ccaccagcgg atgatggcga ccagcttctc gccgatctcg cgcgttgccg cccggcgtgt
     4501 cagcaggtgc tccgcgccat agatctccgg cgtctcggac aggatggctg tgcccccgtg
     4561 tttcaccagc agatcggcgg cgacgccgag cgccgggttg gccgtgatgc cggaatagcc
     4621 gtcggacccg ccgcattgca gcgccagcat cagttcggac gcgggttggg tggagcgaac
     4681 cgcccgattg gcgatgggca gcatcgcctt gatggcttcg acaccggccg cgacggtctt
     4741 cttcgtgccg ccgacctcct gaatggtcat ggtgcggaag gtatcgctct cgctcatccc
     4801 ataggcttcc ttccagcggg cgatctgaaa gccttcgcag cccagtccga ccatgaccac
     4861 cccggccatg ttggggtttg cggcatagcc ccattgcgta cgcttgagga cctcgtagcc
     4921 ctcgcccttg atgtcgagcg cgcagccgcc gccgtgcacc agcggaatca cgccgtcgac
     4981 attggggtag tccgcgagga tgccggaacg gttgatctcc tccgccatga acttggcgac
     5041 cgtcgcggag cagttcaccg aagtgaggat gccgatgtag ttgcgggtgc ccacccggcc
     5101 gttggcgcgt ctgaagccct cgaacgtcgc ccgttgcgcc tccggcagca ggccgtcatc
     5161 gcgcgcgtcc gcgcaaaagg cgtagtcccg gtcgaaggcg gcgaaggtgc agttgtgctc
     5221 gtgaatccat tctcccggcg tgatggcgcg cgcggcgaat ccgatgatct gcccgaactt
     5281 gcgcaccggt tcgcgttccg ccaacgccac ccgcgccatc ttgtggccgc gcggaatgcg
     5341 ctccttcgcg gtgatgccct gcgcagccac gcccggctcg atggcatcga ccgcgaccag
     5401 caccgaatcg gtgtcgtgca gcttgagcac tcggggttgg gtcctgggcg agacgggcgc
     5461 gttcatggct gggtccttgc cggcacggtg ggcggatgtc atgttgtccg acaacctagc
     5521 gggcgctcgc gcccggtgca aacgctcctc gaccggaagc cgccatgcgc atcgacctga
     5581 cgcctgagaa cacgctgccc catgatggaa cctccggcgc gcttgccggg cgtgtctgga
     5641 ggcccgagca caaggggcca agcgtcgcgg cgatccgcgc caacggcgtc ttcgacatct
     5701 cccgcagctt cccgaccatg cgcgatctct gcgagacggc agaccctgcg agggcgctgg
     5761 acgatgcggc tggcgagcgc atcggcgacc ttgccgcgat tctctccaac acgccggagg
     5821 acagtcgcga tccgtcgaag ccctggctgc tcgctccgat cgacctgcag gccgtgaagg
     5881 cagccggcgt gacttttgcc gtgtcgatgc tggagcgggt catcgaggag cgcgcgcgcg
     5941 gcaatccggc ggcggcggca gaaatccgca aggaggtgac gcggctcgtc ggagacgatc
     6001 tgtcgaaact caagcccggg tccccggagg cgatgcgcct caaggacgtg ctgatcgcgc
     6061 aaggcgtctg gtcgcagtat ctggaggtcg gcatcggtcc cgacgccgag attttcacca
     6121 aggcgcaggt gctctcggcg gtcggcaccg gggcggacgc cggcctgcat ccgatctcga
     6181 cctggaacaa ccccgagccg gaagtggtca ttgtcgtggc ttcggacggg cggatcgtcg
     6241 gcgcgacgtt gggcaacgac gtgaacctgc gcgacgtgga gggccgctcg gcgctgctcc
     6301 tgtccaaggc gaaggacaac aacgcctccg ctgcggttgg accgttcctc cgcttcttca
     6361 atgcgacctt ttcgctcgac gacgtgcgcc gcaccgaggt cgcattgcgt gttgaaggac
     6421 aggacgggtt cctgcttgag ggcgcatcca atgtcgcgaa gatcagccgc gatccggccg
     6481 atctcgcccg tcagcttctc ggccggcatc accagtatcc ggacggggcg gtgctttatc
     6541 tcggcaccat gttcgcgccg gtgaaggacc gcgacggcgc cggccaggga ttcacgcaca
     6601 agctcggcga tgtcgtcacc atcgcggcgc cgaagctagg ccgccttgtc aaccgcatga
     6661 cccattcgga caaggccgcg ccctggacct ttggcgcgag ccatctcatg cgcaacctgg
     6721 cgaagcgggg actgctgtag gagcggcagg ccgtcgtcgt cgcacggacg ggcagttgcc
     6781 ggctgggcct agcccacccg ctccagcgcg atcgccgcgc cctgaccgac gcccacgcac
     6841 atggtggcga gcgcatagcg cccgccgcgc cgctgcaact cccgcgccgc cgtgccggta
     6901 atccgggtgc ctgacatgcc gagcggatgg ccgagcgcga tggcgccgcc attggggttc
     6961 acgtgctcgc catcgtcggc gacaccgagc tggcgcaggg tggccagccc ctgcgaggca
     7021 aatgcctcgt tgagctcgat cacgtcaaaa tcactcatct tcaggccgag cctgtcggcc
     7081 agcagcctgg acgcgggcac cgggccgatg cccatgacgc gcggcgcgac accggcggca
     7141 gcgaagccca gcactttcgc gatcggcttc agcccatgct tcctggccgc atccgccgaa
     7201 gcgagaatca tcgccgccgc gccgtcattg acgccggaag cgttgccggc ggtcaccgtt
     7261 ccctccggct tcacgatggg gcggagcttc gccagcatct ccgccgtagt gtcggcgcgg
     7321 ccgtgctcgt ccttcgcgaa gacggtgacg ttgcccttgg cgtccttgat ctcgaccgga
     7381 acgatctcgc cgtcgaagta gccggcggcc tgcgccctgg cggcgcgctg ctgcgagcgc
     7441 agcgcgaagg catcctgatc ggcgcgggaa atctgatggt ccgccgcaac attctcggcg
     7501 gtctccggca tggaatcgac gccatactgt ttcttcatca gcggattgat gaaacgccag
     7561 ccgatggtgg tgtcgaagat gtcggcggaa cgtgcgaagg cggtctcggc cttgcccatg
     7621 acgaacggcg cgcgggtcat ggactcgacg ccccccgcga gcacgatatc cacctcgccg
     7681 gcgcggatgg cgcgcgcgcc ctgacccacc gcgtccatgc cggaggcgca caggcggttc
     7741 agcgtgacgg ccggaacggt ctccggcagg ccggccagca acgcggccat gcgtccgaca
     7801 ttgcggttgt cctcgccggc ctggttggcg cagccgaaga agatttcctg gacggcatcc
     7861 ttcaggccgg gatggcgttt cgccagttcg cgcacgggga tcgccgcgag atcatcggcc
     7921 ctcaccttcg cgagtgaccc gctatagcgg ccgatggcgg tgcggacata gtcgcagatg
     7981 tagacgtcac gggacatggc ttgctcctgt tgtccgcctc atgccgcaaa gcggcagggc
     8041 gggcaaggga ggctgcggcc actggcgcgc tcatcctgtt ccgccgaatg cctccgggcc
     8101 ggcgtcgagc agacggtcgt cgccggagaa cagcgcttcg aggatcgcac tctcgcgacg
     8161 gatgatccgt gccagaaacg cgtcggcccg gcccatcttg cggaaggcgt cgtatccgct
     8221 ttgcaggaac gcctgcagct ccgccaggcc ggccagttcc gccggcttgc gcatcatcct
     8281 gagggcggtg ccggcgaagg cgcgtcgcgt cagccgatcc agtgcgtgcc cgagatgatc
     8341 gaccagcccg atctgccgga gccggtcgtc cttgcggccc accttgcggt aggcgttgcc
     8401 gtagacgacc gcgtccagcg cccggacctt gcggcccagg gccttgacca tggcggcgtc
     8461 gaggtcctca gatagcgcgt cgagttccac cgcatcggcc accgtttcca gcccgccttc
     8521 cggcagcagg cgggtgagcg tgggcgccac gcgcgccaca tcggcatcac gttgggccgt
     8581 gtccttcagc ccgtagatct catcgaggaa aaagcgcgcg gcgtcgtggt gtcgcgcatc
     8641 ggcaagcagg tccgcgtgcg tgcgtgccag gcgttccgat tgccagcggc gaaggaaa
//
LOCUS       DAOMXN010000098         8676 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_4278_length_8676_cov_10.585083, whole genome shotgun sequence.
ACCESSION   DAOMXN010000098 DAOMXN010000000
VERSION     DAOMXN010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 8676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8676
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4278_length_8676_cov_10.585083"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PK812_08170"
     CDS             <1..327
                     /locus_tag="PK812_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HRJ69568.1"
                     /translation="LDAASMDPAAVARERALQADKAAASGKPANVIEKIVDSAMKTFY
                     KENALVDQPFIHDPAKTVAQAVNEAGKAAGAPITLKGYVRFALGEGIEKEQTDFADEV
                     KKAGGA"
     gene            400..1119
                     /gene="pyrH"
                     /locus_tag="PK812_08175"
     CDS             400..1119
                     /gene="pyrH"
                     /locus_tag="PK812_08175"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HRJ69569.1"
                     /translation="MSLKPLYKRALVKLSGEALMGSESFGIHQPTVKQICQDLVGAAD
                     IGVEIAVVVGGGNIFRGAQMSGMGLTRPAADSMGMLGTVMNAIALEAQIEALGHRARA
                     MSAVAMPSVVETYTRQQAGDHLSKGRIVILAGGTGNPFFTTDTGGALRAAELGCDVML
                     KATQVDGVYSADPKKDPTARRYDQVSFDEAIARDLKVMDTAAFALARDARLPIIVFSI
                     REQGAIAAVLAGQGRGTVVRA"
     gene            1186..1749
                     /gene="frr"
                     /locus_tag="PK812_08180"
     CDS             1186..1749
                     /gene="frr"
                     /locus_tag="PK812_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003598054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HRJ69570.1"
                     /translation="MAATTFDLNDIKRRMQGAVASLKHDLNSLRTGRASANLLDPIEV
                     EAYGARMPLSQVATVSVPEPRLISVQVWDRSMVHAVEKAIRDSSLGLNPNTEGQVIRL
                     RIPELNEQRRKEMVKVAHKYAEEARIAVRHVRRDGLDLLKKLEKDGKMGKDDATRSSE
                     QVQKATDQTIADVDAALVHKEKEIMQV"
     gene            1767..2525
                     /locus_tag="PK812_08185"
     CDS             1767..2525
                     /locus_tag="PK812_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HRJ69571.1"
                     /translation="MENAARPLAEAQPGADSGSGPYGLKHVAIIMDGNGRWAQKRGLP
                     RTLGHHAGVEALRRVVRHAGDIGLPFLTIYSFSSENWSRPASEVADLMGLLKRFIRND
                     LADLHSRNVRVRIIGERGSLAPDIRGLLDEAERMTERNTALTLVVAFNYGARQELAAA
                     AQALAAEAVAGRIAPQEITPEAISARLLTVGIPDPDLIIRTSGEQRLSNFLLWQAAYT
                     EFVFTPTLWPDFDGRALDEALRDYAGRTRRFGGL"
     gene            2522..3391
                     /locus_tag="PK812_08190"
     CDS             2522..3391
                     /locus_tag="PK812_08190"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-archaeol synthase"
                     /protein_id="HRJ69572.1"
                     /translation="MTAGSGLRSKSELFQRVVSALVLAIVALATAYLGGWLFALVWAV
                     LAVAIVYEWEKIVAPEQSPTMAIAMAAIVAIAAIVFRDAGVSPSFFIVAGAGAAAVLL
                     VIAVRAREDRAKALWLCAGLVYASALLASVVVVRSAGLIGFFAILFLFIVVWLTDIGA
                     YFAGRTFGGPKFAPTISPKKTWSGVIGGVACGVLCGLFVVWMASHFVRVQVSFAHAVL
                     AFFLSVATVYGDLFESFLKRRFGVKDAGSIIPGHGGFLDRLDGFTAAAILAGLIGTAR
                     GGAYGAAQGLLAW"
     gene            3385..4578
                     /gene="dxr"
                     /locus_tag="PK812_08195"
     CDS             3385..4578
                     /gene="dxr"
                     /locus_tag="PK812_08195"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HRJ69573.1"
                     /translation="MVSGAPSSLTLLGATGSIGSSAVAVIRANPGRFRVEAVAGGRNA
                     GGLAKLARDLGATCAAISDESAYRDLKNELFGSGIAPLGGASGVCEAAARPSDRVVAA
                     ITGVAGLQATCVALERGATVALANKEAMVSAGKAVLAVARKGRATILPLDSEHNALFQ
                     ALGGAPIEAVARMTLTASGGPFRTWTAERIAAATLEQALAHPNFAMGSKITVDSASMM
                     NKGLELIEAHFLFGLPESRLDVVVHPQQLIHGLVTFADGSVNAGMAMPDMRVPMAHCL
                     TYPERCDSGARGLDLLTIGRLDFEAPDDVRFPALRLAREALKAGKSAPTVLNAANEVA
                     VEAFLARKIRFCDIAATVETVMNLPGLADQREPETVEEAISVDHVSRFHAAQALSLRA
                     RRVTN"
     gene            4777..5904
                     /gene="rseP"
                     /locus_tag="PK812_08200"
     CDS             4777..5904
                     /gene="rseP"
                     /locus_tag="PK812_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HRJ69574.1"
                     /translation="MVGGFASNLAGYLIPFFFVLALVIFVHELGHYLVGRWCGVAVEA
                     FAIGFGPKVVGWTDSHGTVWKLCAVPLGGYVKFKGDLNAASVPDSEAMAQMSAQERAE
                     SFPFKPVWQRAAIVAAGPIANFLLAIVIFTGVFMFNGRWIVEPIIGVVVPGSPAETAG
                     FLAGDRILSINGERMEGFDSVRHAVSSSREPSLSIEVDRAGVLKTLIAEPRVTEQKTP
                     FGMLRARTLGIGASSLVEHHRLVKYGVVDSFGLSIDQIRSVLTQTVDYIGGLFTGREK
                     ADQLSGPIRIAQVSGKVAEAGFVSLLSLAAILSISIGFINLLPVPLLDGGHLLFYSIE
                     AVFRRPLSRRTQELGFKVGLALVLMLMVFVTINDIVQIRKG"
     gene            6113..8671
                     /gene="bamA"
                     /locus_tag="PK812_08205"
     CDS             6113..8671
                     /gene="bamA"
                     /locus_tag="PK812_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020092985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HRJ69575.1"
                     /translation="MKSAAAAASCLRVLRRVAGGLLVAAALVTIPVAVSAPAAAQTIV
                     VQGNTRTDADTIRSYVQPRSGETMDAARVDQAIKDLFATGLFSDVKIRRDGGRIVVTV
                     VENTMINRVAFEGSSKLKPDQLQGEVQSRSRGPFSKALVDADVARLKEIYARSGRGDA
                     QIDARITNLPNGRVDVTFVIREGSKTGVAAIEFVGNNKVSSRRLRGVMETTESGLFGW
                     LKTSDVYDPDRLAADVDRVRRYYLKNGYADFRVLSSTADYDAARKGFVITIAVEEGEQ
                     YRIADVSVDSRVRDVEQAQLQRVVRTSRGDIYNAEAVEKSVEAIAGETAKRGYAFAQV
                     RPRGERDPSTQTVRIVYSVEEGARAYVERINIRGNTRTRDYVIRREIDLAEGDAFNKI
                     AVDRAERRLKALGFFRTVRVSNEQGSSPDRVIVNVDVEDQSTGSLALSGGYSTSDGFM
                     AEASITESNFLGRGQFVKLSGSNGQRSRGFEFSFTEPFFMDRRLSAGFDFFSKQTFNS
                     QYARYETRTSGGTLRFGLPLTEEFAIGLRYTLYSQKITIPNTVTRPYNDCTFGLGAGI
                     TTFNNINYCMYNGEASVAVKQAQGTTLTSLVGLTFAYNTLDNPKNPTSGWSAEMRPEI
                     AGLGGDSRFARVTGNARYYYPLTEDITGMLRIQGGHIMGYGGQSGLYGGSVRMIDHFF
                     MGPELVRGFASGGIGPRDLNGDPKGNALGGTTYFGATAEAQFDLPFVPRELGLKAALF
                     ADAGTLFGYRGAKYFDVNRSGVIDGIGANFSCPAPGIGVIQQFQPECVNVRDKKLWRS
                     SVGASLLWQSPLGPIRFDYGFALTKDEGYRDPITGMRVGGDRTQAFRFSGGARF"
BASE COUNT         1455 a         2601 c         2936 g         1684 t
ORIGIN      
        1 ctcgacgccg ccagcatgga tccggcggcg gtggcgcgcg agcgcgccct gcaagcggac
       61 aaggcggcgg cctccggcaa gccggcgaat gtgatcgaga agatcgtcga tagcgcgatg
      121 aagaccttct acaaggaaaa tgcgctggtc gatcagccct tcatccacga tccggcaaag
      181 acggtggcgc aggctgtgaa tgaggcggga aaggctgccg gcgcgccgat cacgctcaag
      241 ggctatgttc ggtttgcgct tggcgaggga atcgagaaag aacagactga cttcgccgac
      301 gaagtgaaga aggctggcgg cgcctgaggg cgtgcgacac cggctttgac caggggcggc
      361 gaaagccgcc ctttttcttt tggcgccgtg gaggccgaga tgtccctgaa acccctctac
      421 aagcgcgcgc tggtgaagct ttcgggcgag gccctgatgg gctcggagag cttcggcatc
      481 caccagccga ccgtaaagca gatctgtcag gacctggtcg gcgccgccga tatcggcgtt
      541 gagatcgcgg tcgtggtcgg cgggggcaac atctttcgcg gtgcgcagat gtccggcatg
      601 gggctcaccc gtcctgctgc ggattccatg gggatgctcg gcacggtcat gaacgccatc
      661 gcgctcgagg cgcagatcga ggcgctggga caccgcgccc gcgcgatgtc ggccgtggcc
      721 atgccttcgg tggttgaaac ctacacgcgc cagcaggccg gcgatcacct ttccaaaggg
      781 cggatcgtga tcctggcggg cggcacgggc aatccgttct tcacgacgga taccggcggt
      841 gccctgcgcg cggcggaact gggatgcgac gtcatgctga aggcgacgca ggtggatgga
      901 gtctactccg ccgatcccaa gaaggatccg actgcgcggc gctatgacca ggtgtcgttc
      961 gacgaggcca ttgcgcgcga tctcaaggtc atggacacgg cggcgttcgc gcttgcccgc
     1021 gatgcccgcc tgccgataat cgtattttca atccgcgagc agggcgccat cgccgcggtt
     1081 ctcgccggcc aggggcgtgg tacggtggtg cgagcctaga cagggtgctt gcgggacgcc
     1141 gcttccgggt ctaggggtca gcggcatcga tatccgaggt tcgccatggc cgccaccaca
     1201 ttcgacctga acgacatcaa gcggcgcatg cagggcgctg tcgcttccct gaagcatgac
     1261 ctcaactccc tgcgcacggg ccgcgcctcg gccaatctgc tcgatccgat agaggtggaa
     1321 gcctatggcg cgcgtatgcc gctgtcgcag gtcgccaccg tcagcgtgcc ggaaccgcgg
     1381 ctcatctcgg tccaggtctg ggatcgaagc atggtgcatg cggtggaaaa ggcgatccgc
     1441 gactcgtccc tgggcctcaa ccccaatacg gaggggcagg tgatccgcct gcgcattccg
     1501 gaactgaacg agcagcgtcg caaggaaatg gtgaaggtcg cccacaagta cgcggaagag
     1561 gcgcgcatcg ccgtgcgcca cgtgcgccgc gacgggctcg atcttctcaa gaagcttgag
     1621 aaggacggga agatgggcaa ggacgacgcc acacgctcct cggagcaggt ccagaaggcg
     1681 acggaccaga ccatcgccga cgtggatgcg gccttggtcc acaaggaaaa ggagatcatg
     1741 caggtctgac ctgcggtccg attgccatgg aaaacgccgc aagacccctt gcagaggccc
     1801 aaccgggtgc ggattccggc agcggtccct atgggctcaa gcatgtggcg atcatcatgg
     1861 atggcaacgg ccgctgggca cagaaacgcg gcctgccgcg aacgttgggc catcacgcgg
     1921 gcgtcgaagc gttgcgtcgc gttgtccgcc atgccggcga catcggcctg ccgttcctga
     1981 ccatctacag cttttcttcc gagaactggt cgcggccggc cagcgaggtc gccgacctga
     2041 tgggcctgct gaagcggttc atcaggaacg acctggctga tctgcattcc cgtaatgtgc
     2101 gggtgcgcat cattggcgag cgcggcagtc ttgccccgga tattcgtggg ctcttggacg
     2161 aagccgagcg catgaccgag cggaatacgg ctttgacgct tgtcgtggcc ttcaattacg
     2221 gtgcccgcca ggaactggcg gcggcggcgc aggccctcgc cgcggaagcg gtcgccggcc
     2281 ggattgcgcc acaagagatc accccggagg cgatcagcgc gcgcctgctg actgttggca
     2341 taccggatcc cgatctgatc atccggacgt cgggcgaaca gcggctgtcg aatttcctgc
     2401 tctggcaggc ggcctacacg gaattcgtgt tcacacccac gctgtggcca gatttcgacg
     2461 ggcgggcctt ggatgaggcg ctgcgcgact atgccgggcg cacacgccgt ttcggtggcc
     2521 tatgacggcc ggatcggggc tgcgatccaa gagcgaactg ttccagcgcg tggtttcggc
     2581 gctcgttctg gcgatcgtgg cgctggccac ggcctatctc ggcggatggc tcttcgcact
     2641 ggtctgggcc gttcttgctg tcgccatcgt ctacgaatgg gaaaagatcg tcgcgccgga
     2701 gcagtcgccg accatggcca tcgccatggc cgcgatcgtc gccatcgccg ccatcgtctt
     2761 ccgggacgcc ggagtttctc caagcttctt catcgtggcc ggcgccggtg ccgcggctgt
     2821 gctgttggtc atcgcggtcc gggcgcggga ggacagggcg aaggcgctct ggctctgcgc
     2881 cgggctggtt tacgccagcg cgttgctggc gagcgtcgtg gtcgtgcgct cggcagggct
     2941 gatcgggttc ttcgccatcc tgttcctgtt catcgtggtc tggctgaccg acatcggcgc
     3001 ctatttcgcc gggcgaacct ttggcgggcc gaaattcgcc ccgacgataa gccccaagaa
     3061 gacctggtcg ggcgtcatcg gcggtgtcgc ctgcggcgtg ctctgcgggc tgttcgtggt
     3121 ctggatggcc tcgcacttcg ttcgtgtcca ggtcagtttc gcacatgcag ttctggcatt
     3181 tttcctttct gtagcgactg tttatgggga tttgttcgag tcgtttctta aacgccgatt
     3241 cggtgtgaag gatgccggct cgataattcc cggccatggc ggctttctgg atcggctcga
     3301 cgggttcacg gcggcggcga tcctggctgg cctgattggc acggcccgtg gcggcgcgta
     3361 tggggcggct cagggtctgc tcgcatggtg agcggcgcgc cgtcatccct gacacttctg
     3421 ggggccacgg gctccatcgg ctccagcgca gttgcggtga tccgcgccaa tcccggtcgg
     3481 ttccgggttg aagccgtggc gggcggacgc aacgcggggg ggctggcaaa gctcgcgcgc
     3541 gacctcgggg caacatgcgc cgccatttcg gacgaatccg cctatcgaga cctgaagaac
     3601 gagctgttcg gttccggaat cgcgccactc ggcggcgcca gcggcgtttg cgaggctgct
     3661 gccaggccat cggatcgggt ggtcgcggcg atcacgggcg ttgccggctt gcaggccacg
     3721 tgtgtcgcgc ttgagcgcgg tgcgacggtg gcgctcgcca acaaggaggc gatggtgagc
     3781 gccggcaagg cggttctggc cgtcgcgcgg aagggccggg cgaccatcct gccgctcgat
     3841 tcggagcata acgcgctgtt tcaggcgctc ggtggcgcgc cgatcgaggc ggtcgcgcgc
     3901 atgacgctga cagcatccgg tggcccgttt cgaacctgga ctgcggaaag gatcgccgcc
     3961 gcgacgctgg aacaggcgct ggcgcatccg aatttcgcca tgggttcgaa aatcaccgtc
     4021 gattccgctt cgatgatgaa caaggggctg gaactcatcg aggcgcattt cctgttcggc
     4081 ctgccagaat ccaggctgga cgtggtggtg cacccgcagc agctcattca cgggctggtc
     4141 accttcgccg acggcagtgt gaatgcggga atggcgatgc ccgacatgcg ggttccgatg
     4201 gcccattgcc tgacctatcc cgagcgctgc gattccggcg cgcgcggcct cgacctgtta
     4261 accattggcc ggcttgattt cgaggcgccg gacgatgtgc gctttcctgc tctgcggttg
     4321 gcccgtgagg cgctgaaagc cggaaaatcc gcgccaaccg tgctgaatgc ggcgaacgaa
     4381 gtcgcagtcg aggcgttttt ggccagaaaa atacggttct gcgacatcgc ggcaaccgtg
     4441 gagacggtca tgaacctgcc gggtttggct gatcaaaggg aacccgagac ggtcgaggag
     4501 gcaatctctg ttgaccatgt ttcgagattt catgccgcgc aggcgttgtc gcttcgcgcg
     4561 cgccgtgtca cgaattagcg ttttccggcg tggggacgcc gcaaggccag cgggcgccat
     4621 gggcgatcgc tggctccact gggtgatttt ccgttccgga aggaagggcg aaatctccaa
     4681 aaggggtcga attccagcgg cttgccgtag gggattcggt ggttggcgcc tctttgcagg
     4741 cgcgcggagg atggtgggtt gacggatatt ctgagcatgg tcggcggatt cgcctcgaat
     4801 ctggccggct atctgatccc gttctttttc gttctggcgc ttgtcatctt cgtgcacgag
     4861 ctcggccact atctggtcgg gcgctggtgc ggcgtggcgg tggaggcgtt cgcgatcggt
     4921 tttggaccga aggtcgtggg ctggaccgac agtcacggca cggtctggaa gttgtgcgcg
     4981 gtgccgctgg gcggctacgt caagttcaag ggagacctga acgccgcgag cgtgccggat
     5041 tccgaagcca tggcgcagat gtcggcgcag gagagggcag agtccttccc gttcaagccg
     5101 gtctggcaac gcgcggcaat cgtcgcggcc ggtcccatcg cgaatttcct gctggcgatt
     5161 gtgatcttca ccggcgtgtt catgttcaac ggtcgctgga tcgtcgagcc gatcatcggt
     5221 gttgttgttc ccggttcccc ggccgagact gccgggtttc tggccgggga tcgcatcctg
     5281 tcgatcaacg gggagcggat ggaggggttt gattctgtcc gtcacgccgt gtcgtcgagc
     5341 cgcgagccat cgctcagcat cgaagtcgac cgcgccggcg tgctgaaaac cctgatcgcc
     5401 gaaccgcgcg tcaccgaaca gaaaacgcct ttcgggatgt tgcgggcgcg gacactcggg
     5461 atcggcgcat cgagtctcgt cgagcaccac aggctggtga aatatggcgt tgtcgacagc
     5521 tttggcctgt cgattgacca gattcgctcg gtgttgacgc agaccgtgga ttacatcggc
     5581 gggctgttca ccggtcggga aaaggcagac cagctttccg gcccgatccg cattgcccag
     5641 gtctccggaa aggttgccga ggcggggttt gtttcgctgc tgtcgctcgc ggccatcctg
     5701 tcgatttcca tcggcttcat caatctgctg ccggtgcccc ttctggatgg cggtcacctg
     5761 ctgttttact cgatcgaagc ggtgtttcgc cggcccttga gccgtcggac ccaggagttg
     5821 gggttcaaag tggggctggc gctggtgctc atgctcatgg tctttgtcac gatcaatgac
     5881 atcgtgcaga tccgaaaagg ttgagtgctc ctccgggcgt ggcgcagcgg tcacgcgcag
     5941 gcgcaaaaag aaggttctcc atggctttgg cagttgcatt ggccggatga gcggttagaa
     6001 gcagacgata cggcggtttg cgaaagccgg gattcccggc gtgcgcccaa gagcaagaaa
     6061 ctgacggttc cgcggaatag gcgaaacgat gatgatgaag tttgttcggg acatgaaaag
     6121 tgctgcagcc gcggcatcct gtttgcgcgt gctgcggcgc gtcgcgggtg ggctgctggt
     6181 agcggcggcg ctggttacga tcccagtcgc cgtttccgca cctgcggcgg cgcagacgat
     6241 cgtggttcag ggcaataccc gcaccgacgc ggatacgatc cgttcttacg ttcagccccg
     6301 ctctggcgaa acgatggatg ccgcccgcgt cgatcaggcc atcaaggacc tgttcgccac
     6361 cggcctgttc tcggacgtca agattcggcg cgatggcggc cgcatcgtgg tcaccgtggt
     6421 cgagaacacg atgatcaatc gcgtggcatt cgagggctcc agcaagctga agcccgacca
     6481 gcttcaggga gaagtgcaga gccggtcgcg cggtcctttc agcaaggcgc tggtcgatgc
     6541 cgacgtcgcg cgcctgaagg agatctacgc gcgttccgga cgcggtgacg cgcagatcga
     6601 cgcccgcatc accaacctgc caaacgggcg ggtcgacgtg accttcgtga tccgcgaggg
     6661 cagcaagacc ggcgtggcgg cgattgaatt cgttggcaac aacaaggttt cgtcgcggcg
     6721 gctgcgcggc gtcatggaga cgacggagtc gggcctgttc ggctggctga aaacctccga
     6781 cgtctatgat cccgaccgcc tcgcggcaga tgtggaccgc gtccgccgct actacctgaa
     6841 gaacggttac gccgacttcc gggttttgtc ttcgacggcg gactatgacg cggcccgcaa
     6901 gggttttgtg atcacgatcg ccgtggaaga gggtgagcag tatcgcatcg ctgacgtctc
     6961 ggtcgattct cgcgtccgtg atgtggagca ggcccagctt cagcgcgtgg ttcgcacctc
     7021 gcgtggcgac atctacaacg ccgaggcggt tgaaaagtcg gtggaggcaa tcgccggcga
     7081 gaccgccaag cgcggctacg cgttcgctca ggttcgcccg cgcggcgagc gcgatccctc
     7141 gacgcagacc gttcgtatcg tctattcggt cgaggaaggc gcgcgcgcct acgtcgagcg
     7201 tatcaatatc cgtggcaaca cgcgcacgcg ggactatgtg atccgtcgcg agatcgacct
     7261 cgccgaaggg gacgccttca acaagatcgc ggttgatcgc gccgagcgca ggctcaaggc
     7321 gctgggcttc ttcaggaccg ttcgcgtttc gaacgaacag ggatcgagcc cggatcgcgt
     7381 catcgtcaac gtcgatgtcg aggatcagtc cacgggttcg ttggcgctgt ccggcggtta
     7441 ttcgacctct gatggtttca tggcggaggc ctcgatcacc gagtcgaact tccttggacg
     7501 cggccagttc gtgaaactgt ccggtagcaa cggccagcgc agccgaggct tcgagttctc
     7561 gttcactgag ccgttcttca tggatcggcg gctttccgcc gggttcgatt tcttctcgaa
     7621 gcagacgttc aattcgcagt atgcgcgcta tgagacgcgc acgagtggcg gaacgttgcg
     7681 cttcgggctg ccgctgaccg aggaatttgc gatcggcctg cgctacacgc tctattcgca
     7741 gaagatcacc attccgaaca cagtcacacg tccctataac gactgtacgt tcggactcgg
     7801 ggccggcatc accacgttca acaacatcaa ctactgcatg tacaacggtg aggcatcggt
     7861 tgcggtcaag caggcgcagg gcacgacgct tacttctctg gtcggcctga cgttcgccta
     7921 caacacgctg gacaacccga agaacccgac cagcggatgg tctgccgaga tgcgtccgga
     7981 aatcgccggc ctcggcggtg atagccgctt tgcccgcgtc accggcaatg cccgctacta
     8041 ctatccgctg acggaagaca tcaccggcat gctgcgcatc cagggcggcc atatcatggg
     8101 ctatggcggc caatccggcc tctacggcgg ctccgtgcgc atgatcgacc actttttcat
     8161 ggggcctgag ctggtgcgcg gatttgcgtc cggcggtatc gggccgcgtg atctgaatgg
     8221 cgatccgaaa ggcaatgcgc tgggcgggac gacctatttc ggagccacgg ctgaagcgca
     8281 gttcgacctg ccgttcgtgc cgcgtgaact cggcctcaag gccgcgctgt ttgccgacgc
     8341 cggaacgctg ttcggctacc gtggcgccaa gtacttcgat gtcaaccgga gcggcgtgat
     8401 cgatgggatc ggcgcgaatt tctcgtgccc cgcgccgggc attggcgtga tccagcagtt
     8461 ccagccggaa tgtgtgaatg ttcgcgacaa gaagttgtgg cggtcctcgg tcggcgcgag
     8521 cctgctgtgg cagtctcctc tggggccgat ccgctttgac tatggcttcg cgctcaccaa
     8581 ggacgaaggc taccgtgatc ccatcaccgg catgcgtgtc ggcggtgatc gcacgcaggc
     8641 cttccggttc tccggcggcg cgcgcttctg aggcgc
//
LOCUS       DAOMXN010000099         8652 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_4298_length_8652_cov_11.091427, whole genome shotgun sequence.
ACCESSION   DAOMXN010000099 DAOMXN010000000
VERSION     DAOMXN010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 8652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8652
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4298_length_8652_cov_11.091427"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..2386)
                     /locus_tag="PK812_08210"
     CDS             complement(125..2386)
                     /locus_tag="PK812_08210"
                     /inference="COORDINATES: protein motif:HMM:NF012409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HRJ69576.1"
                     /translation="MALVDQSGIVGKVRDRRISIELERLLERAAEAAGVDVVRVTSGG
                     QPGTTGKSVGSTRHNGGRAADLQLVVDGRTLEFTDEDGGDTVEAFVTAAAALGANGIG
                     AGVAYMGSRTLHVGFGETPDDHRRIVWGYKGKSKNAPRWLREAAERGWDNPSIIDELS
                     NGTKHSHQHDGEEEEERGGLHREEPPPRRRHGWRDPDFSEMGGGVHDASIRLPDFGPL
                     FPNANPEVMAELRDNGARALARYGILQSERRFAFFMAQLAHESGGFRTLQEGLRYSAN
                     RLMAVWPKRFPTAALASAYAGNEQKLANMVYGGRNGNGPEPTGDGYRYRGRGLIQLTF
                     RDNYREVGRIVDLDLERNPDLAADPEHVWTVACGYWTMRKLNRYADNNDFLALTKTIN
                     GGINGLADRQKHLARVSQFLARVGFTPVGEEAAAPYSTGRSGSGGGSMHRDDIDLNSD
                     DLHFGMPKNPHVESIQKQLNALGFYLGAEDGIFGKNTRAQVMAFQAEHGLATTGIVDA
                     AFRSVLTSASRRTVEGPRQGADEKSMIRDRSRIVYETSWNRLFGKVFAGIGGVGLLNS
                     GVMNTVGGSLGSAGATAKTVVANPAQTMGQIREVLQHATGANGTSPQVALDVVRDILA
                     KQNEVALSASDAIQLAARIRDAIGLVKTTGAEHAQSAQAAVDIIRNLVGKKAEPAASA
                     AQNIFELWAKNAGGGNVSAIADVLGQLAGTLLPGATGSAVTLGLGALSWLISNRVISS
                     RIADHRSGKHLGR"
     gene            complement(2597..3289)
                     /locus_tag="PK812_08215"
     CDS             complement(2597..3289)
                     /locus_tag="PK812_08215"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRJ69577.1"
                     /translation="MGDSGVLNLSVEAAYDRWAKAYDRADNPMVFGATRALAATTGAM
                     VGRDVFEFGCGTGRNLAFLKAAGARSLAGCDLSEAMLAEARGRDPAFVLFRHDMREPL
                     PRPAASADFVLFCLALEHVADLAPPLAQARRILRPGGEIAIVEIHPFVSLTGVAAHFR
                     DGETEIRMPAYPHRFADYLDAFIALDLRVSTCREWRPLDLGPDVPSRALKRGAAYPLV
                     VEWRLRAARLEG"
     gene            complement(3294..4187)
                     /locus_tag="PK812_08220"
     CDS             complement(3294..4187)
                     /locus_tag="PK812_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009492374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRJ69578.1"
                     /translation="MNPRHFAIIVVICLIWAGNTVLSRIIVADLAIPPFFYSTLRFCL
                     VALLLFPWLRHVPRPFWRILAVSFLISGVGFGLFIAGMQDATPSSASIVGQVAVPATT
                     LLSVLVLGETIRWRRITGIALAVIGIIILMYRPGETQASWGLWLIILSSLMAAAGVIV
                     IKQMLGVAPLQYQAWTALLSILPLAGLSWGLESNQWEIARMAGWKLVAALLFVAIMVS
                     IVSHSLYFWVLQRNDANLVAPLMILNPLFSVILGIWITGDQFDLRMSIGAIITLAGVL
                     LILLRPSAVLPLANAILDRFR"
     gene            4305..4964
                     /locus_tag="PK812_08225"
     CDS             4305..4964
                     /locus_tag="PK812_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018261213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRJ69579.1"
                     /translation="MTATPTPPLQIGIVPVTPFEQNCMIIKCSATNKAAIVDPGGDAE
                     EILAAVKQFGVDVEKILLTHGHLDHVGGVKDVVAALKVPVEGPHVADKWLVEHAEESA
                     RKYGFPPGNMKNAWSDRYVEEGETVSVGNLTFDILFIPGHSPGSVVYVSREAPIAIVG
                     DVIFQGSIGRTDFPGCDHDALITGIKTKLFALPDTVTCLPGHGPATTIGEERRTNPFV
                     R"
     gene            5068..5484
                     /locus_tag="PK812_08230"
     CDS             5068..5484
                     /locus_tag="PK812_08230"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007009427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRJ69580.1"
                     /translation="MSAMIDFARKVLAAQPANTLVGAELTQFEPGRATLELPIRPHHL
                     QQHGVVHGGLVSFMADNALTFAGGSVLGDVMTLEFKINYMRPAKGERLIARARVTGSG
                     RTTAVCHCDLYALEAGAEKHCATAQGTIFKLEGKGG"
     gene            5561..5812
                     /locus_tag="PK812_08235"
     CDS             5561..5812
                     /locus_tag="PK812_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69581.1"
                     /translation="MRNGLIIALSAGCLMLAPAALAQQGPGMGPVAAACGDDIAKLCP
                     GKEHGRGGEVRDCMEANKAKVSAACKKALETTGWGRKQQ"
     gene            complement(5882..6274)
                     /locus_tag="PK812_08240"
     CDS             complement(5882..6274)
                     /locus_tag="PK812_08240"
                     /inference="COORDINATES: protein motif:HMM:NF018684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HRJ69582.1"
                     /translation="MTGAGRKASTGLCALFLLSVMPALAQEECRNAATERDMGACLAR
                     KTQGQGREMAAAIKTLRERYTEPSEKAFGPAMDKAQRAWIAWRDAECAFRNFESRQGT
                     GYGNILATCVSDLNAERLKTLKDMVDSP"
     gene            complement(6285..7280)
                     /gene="glpX"
                     /locus_tag="PK812_08245"
     CDS             complement(6285..7280)
                     /gene="glpX"
                     /locus_tag="PK812_08245"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HRJ69583.1"
                     /translation="MTEIVVTEDRIIERILTMELVRVTERAAVASARLRGRGDEKAAD
                     QAAVDAMRRELNKLPIDGEVVIGEGERDEAPMLFIGERVGTKKGPKVDIAVDPLEGTT
                     LCAKDMPGSIAVMAMAQAGTLLYAPDVYMDKIAIGPGYPKGIVDLDASPEDNIMAVAK
                     AKGCKPGEVTALVMDRPRHAELIARLRTIGCSIRLITDGDVVGVIQTTQPEKTGVDIY
                     LGIGGAPEGVLAAGALRCVGGQMQGRLILDTPEKVARAAKMGVKDPKKKYDLNELASG
                     DVVVAATGVTDGALLRGVKFYGELIETETIIYRSLTGTVRRIVGEHRDLKKFHLG"
     gene            7540..8241
                     /locus_tag="PK812_08250"
     CDS             7540..8241
                     /locus_tag="PK812_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69584.1"
                     /translation="MLRAATLLFLLSPALAGQPGVAGDCAGMPSVQRPCASSEIDRLT
                     REVETAYSVALNGIGSAGRSALKRDQESFRRILDQSLSLGDQDAETLLTLRRDLLDSI
                     RPSRGEWTGSWANAFGTIEILATSGGNYHVRLHAADPGAGGWVCEFEDSAILAPRGIA
                     MIVGAESAADDHGGPNEGWTLAFRLAGDILMVEPLRPKGFPGAMPFCGKGGNVAGNFF
                     AQDHGLALRRTSERK"
     gene            complement(8248..>8652)
                     /locus_tag="PK812_08255"
     CDS             complement(8248..>8652)
                     /locus_tag="PK812_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HRJ69585.1"
                     /translation="GPGAGGGATGSAVVADIVDIARGISVSVFGRPADALEKLVRAPI
                     RRHEGGYYIRLQVKDKPGAAAAIATRMAERSISLESILQKGGRDRAASGQSVPVVLIT
                     YATSESAIRSALARIADDGVVEGDPQVIRIER"
BASE COUNT         1522 a         2878 c         2806 g         1446 t
ORIGIN      
        1 agccgcgcgc gccagcgccg accgaggaac gacgaggcaa cgtcgaggaa gggcatcatc
       61 gggtccgcct tacaggaaag ccggccggca tcaaagccgg ccggcccgtg acgggccgcc
      121 ccgcctagcg gccgaggtgc ttgccggacc tatggtccgc gatgcggctc gagatgacac
      181 ggttgctgat cagccagctc agcgcgccaa gtcccagggt cacggcggag ccggtggctc
      241 cgggaaggag cgtgcctgcc aactggccca ggacgtcggc gatcgccgaa acgttgccgc
      301 cgccagcgtt tttcgcccag agttcgaaga tgttctgggc agccgaggcc gccggttcgg
      361 ccttctttcc aacgaggttt cgaatgatgt cgacggcggc ctgtgccgac tgggcatgtt
      421 ccgctcccgt tgtcttgacg agaccgatcg cgtcgcgaat ccgggccgcc agctggatgg
      481 cgtccgacgc cgacagcgcc acctcgtttt gcttcgccag gatgtctcgt acgacatcca
      541 gcgccacttg cggggaggtg ccattggcgc ccgtggcgtg ctgcagcacc tccctgatct
      601 gccccattgt ctgcgccggg ttggcgacca ccgtcttggc cgtcgctccg gcgctgccca
      661 gcgaaccgcc gacggtgttc atgacaccgc tgttcagcaa gccgacgccg ccgatgccgg
      721 cgaacacctt gccgaacagc cggttccagc ttgtctcgta gacgatgcgc gaccggtcgc
      781 ggatcatgct cttctcgtcg gcgccctggc gcgggccttc gacagtccgg cggctcgcgc
      841 tggtcaggac cgaacggaac gccgcatcga cgatgccggt cgtcgcaagc ccatgctcgg
      901 cctggaacgc catcacctga gcgcgcgtgt tcttgccaaa gatgccgtcc tcggcgccca
      961 gatagaagcc caaagcattc agttgcttct ggatggactc cacatgcggg ttcttgggca
     1021 tgccgaagtg aaggtcatcg gaattgaggt caatgtcgtc gcggtgcatc gatccgcctc
     1081 ctgagccaga acggccggtt gaatacgggg cagcggcctc ctcgccgacc ggcgtgaagc
     1141 cgacgcgcgc caggaactgg ctgacacgcg caaggtgctt ctggcgatcg gcaaggccgt
     1201 tgatgccgcc attgatcgtc ttggtcagcg cgaggaagtc gttgttgtcg gcgtagcggt
     1261 tgagcttccg catggtccag tagccgcagg ccacggtcca gacgtgttcc ggatcggccg
     1321 ccagatccgg attgcgctcg aggtccaggt cgacgatgcg gccgacttcc cggtagttgt
     1381 cccggaacgt gagctggatc aggccccggc cacgatagcg gtatccatcg ccggtcggct
     1441 cgggaccgtt gccgttacgc ccgccataga ccatgttcgc cagcttctgc tcgttgccgg
     1501 cgtaggcaga agcaagcgct gcggtcggga aacgcttcgg ccacaccgcc atgagccggt
     1561 tcgcgctgta ccgcaagcct tcctgaagcg tgcgaaaacc gccggactca tgcgcgagct
     1621 gtgccatgaa gaaggcaaag cgccgttccg attgaagaat gccgtagcgt gcgagcgccc
     1681 ttgcgccatt gtcgcgcagt tcggccatga cctcggggtt ggcattgggg aacaagggcc
     1741 cgaaatcagg aagccggatg cttgcatcgt gaacgccacc ccccatctcg gagaaatccg
     1801 gatcgcgcca gccatgccgg cgccgtggtg gtggttcttc ccggtgcaaa ccgccccgtt
     1861 cttcctcttc ctcaccgtcg tgctggtgcg aatgtttcgt cccgttcgac aattcatcga
     1921 tgatggaggg gttgtcccaa ccgcgttcgg ccgcttcgcg cagccagcgg ggagcgttct
     1981 tggatttgcc cttgtagccc cagacgatcc ggcgatgatc gtccggcgtc tcgccgaacc
     2041 cgacatgcag ggtccggctg cccatgtagg cgacaccggc gccaataccg ttggcgccca
     2101 gcgccgccgc ggctgtcacg aaggcttcaa ccgtgtcgcc gccgtcctcg tcagtgaatt
     2161 ccagggtcct gccgtcgacg acgagttgca ggtcggcagc cctgccgccg ttgtggcggg
     2221 ttgagcctac gcttttcccc gtcgtaccgg gctggccgcc cgaagtgacg cgaacgacgt
     2281 caacgccggc agcctcagcg gcccgttcca gcagacgttc cagttcgatc gaaatgcgcc
     2341 ggtcccgcac tttcccgacg atcccggatt ggtcaacaag tgccatggat agccctcccc
     2401 agccacgtag gcctatgccg cgagaggcag tgggccgctt gtcggccccg gaattcaagc
     2461 acgaattcca cgtttcacga tcatttcacg ccgacggcct gcccgaggga cgccggcctg
     2521 gccattgtgc gcgccagtcc ctggcgcatc gaggctctga cgggaatgtc tcatgccggc
     2581 gtcgccccgg acagagtcac ccttccaggc gtgcggcccg cagccgccac tccaccacca
     2641 gcggatacgc cgcgccgcgc ttcagcgcgc gggacggcac atccgggcca aggtccagcg
     2701 gtcgccattc gcggcaggtc gaaacgcgca ggtcgagcgc gatgaacgcg tccagatagt
     2761 cggcgaaacg gtgcgggtag gccggcatgc gaatttccgt ctcgccgtcc cggaagtgag
     2821 cggcgactcc ggtcagcgat acgaagggat gaatctcgac gatggcgatc tcgccaccgg
     2881 gacggagaat gcgccgtgcc tgggccagcg gcggagcaag gtccgccaca tgctccagcg
     2941 cgaggcagaa caggacgaaa tccgcgctcg ccgccgggcg cggcagcggc tctcgcatgt
     3001 catggcggaa gagaacgaag gcgggatcgc ggccgcgcgc ctctgccagc atcgcttccg
     3061 agaggtcgca gccggcgagc gagcgcgcgc cggccgcctt gaggaaggca aggttgcgcc
     3121 cggtgccgca cccgaactcg aacacgtctc ggccgaccat ggcgccggtc gtggctgcca
     3181 gcgcccgcgt cgcgccgaac accatcggat tgtcggcgcg atcataggcc ttcgcccaac
     3241 ggtcgtaggc ggcctcgacg gaaaggttca gtacgccaga atcgcccacg gcgtcaccga
     3301 aagcggtcga gaatggcgtt ggccaggggc aggacggcgc tcggcctcag caggatcagc
     3361 aggacgccgg cgagcgtgat gatcgccccg atgctcatgc gcaggtcgaa ctggtcgccg
     3421 gtgatccaga tgccgagaat gacgctgaac agcgggttca ggatcatcag cggggcgacg
     3481 agattggcat cgttgcgttg cagcacccag aaatacagcg agtgcgagac gatcgacacc
     3541 atgatcgcga cgaacaacag cgcggcgaca agcttccagc ctgccatgcg cgcgatctcc
     3601 cactggttcg actccaggcc ccagctgagg cccgccagcg gcaggatcga cagcagcgcg
     3661 gtccaggcct ggtactgcaa cggcgcgaca cccagcatct gcttgatcac gatcacgccg
     3721 gccgccgcca tcagcgacga gaggatgatc agccacagcc cccaggacgc ctgcgtctcg
     3781 ccgggccgat acatcaggat gatgattccg atgacagcaa gcgcgatccc ggtgatgcgc
     3841 cgccagcgga tggtctcgcc cagcaccagc accgacagca gggtcgtggc cggcaccgcg
     3901 acctgcccga cgatcgaggc cgaagacggc gtcgcatcct gcatgccggc aatgaagagc
     3961 ccgaagccga ccccactgat cagaaagctg accgccagaa tgcgccagaa cgggcgcggc
     4021 acatggcgca gccagggaaa cagcagcagc gcgaccaggc agaatcgcag ggtcgaatag
     4081 aagaacggcg gaatcgccag atccgcgacg atgatgcggc tcagcaccgt attcccggcc
     4141 cagatcaggc agatcacgac gatgatggcg aagtggcgcg gattcatgat gttatagtta
     4201 tagggctaac catcttgcgc aagccgtcgc gcgcttgcgc ggcaagggct tgcctcgcgt
     4261 gccgcgagcg tcgataagcg ccgcagacgc gaggaccgag ccgcatgacc gccaccccaa
     4321 caccgccgct gcagatcggt atcgtccccg tgacgccgtt cgagcagaac tgcatgatca
     4381 tcaaatgctc cgccaccaac aaggcggcca tcgtcgatcc ggggggagac gccgaggaaa
     4441 tcctcgcggc agtcaagcag ttcggcgtgg acgtggaaaa gatcctgctg acgcatggcc
     4501 atctcgacca tgtcggcggc gtcaaggatg tcgtggcggc gctgaaggtt ccggtcgagg
     4561 ggccgcatgt cgccgacaag tggctggtcg agcacgcgga ggaaagcgcc cgcaagtacg
     4621 gcttccctcc cggcaacatg aaaaatgcct ggtccgaccg ctatgtcgag gaaggcgaga
     4681 cggtgagcgt cggcaatctc accttcgaca tcctgttcat ccccggccac tcgccggggt
     4741 ccgtcgtcta cgtgtcgcgc gaggcgccca ttgccatcgt cggcgacgtc atctttcagg
     4801 gatcgatcgg ccgcacggat ttccccggct gcgatcacga cgcgctgatc accggcatca
     4861 agacgaagct cttcgccctg ccggacactg tcacctgcct gcccggccac ggcccggcca
     4921 cgaccatcgg cgaggagcgg cggacgaacc ccttcgtgcg ctgagacgag cagacgctcc
     4981 gatccggcaa cggtttggtg ttgcgcgcat attgttcata tgtaaactgt tggagccacc
     5041 gatacgcacc tgcgggagga atcagccatg agcgccatga tcgacttcgc ccgaaaggtc
     5101 ctcgccgcgc agcccgccaa cacccttgtt ggcgcggaac tcacgcagtt cgagccggga
     5161 cgggcgaccc tggagctgcc gatccgcccg caccacctgc aacagcacgg cgtcgtgcat
     5221 ggcggactgg tcagtttcat ggcggacaac gccctcactt ttgccggcgg gtccgttctg
     5281 ggcgacgtga tgacgctgga gttcaagatc aactacatgc gcccggccaa gggcgagcgc
     5341 cttatcgccc gcgcccgtgt caccggcagc ggacgcacca ccgccgtctg ccactgcgac
     5401 ctctatgcgc tcgaagccgg cgcggaaaag cactgcgcca cggcgcaggg cacgatcttc
     5461 aagctggagg gcaagggcgg gtgaaccctg ccattgacct gcgacaacga cgtttcgcgt
     5521 ggctcaaaat ataaggaact cctaacagaa ggagattccc atgcggaatg gactgataat
     5581 cgcgctttca gccggttgcc tgatgctcgc gccggccgcc ctggcccaac agggaccggg
     5641 catgggtccc gtggcggcgg cttgcggtga cgacatcgcc aagctttgcc ctggcaagga
     5701 gcatgggcgc ggcggcgagg ttcgcgactg catggaggcg aacaaggcca aagtctcggc
     5761 ggcctgcaag aaggcgctgg agaccacggg ctggggacgc aagcagcagt aaatcggggc
     5821 gcgcgttttt ggcggcgccc gtgcgcgtca cgggcgtcgc atctcttggc gccatcgcgg
     5881 cctacggcga atccaccatg tccttcaggg tcttgagccg ctcagcgttg aggtcgctca
     5941 cgcaagtcgc cagaatgttg ccgtagcccg ttccctgccg cgattcaaag ttgcggaagg
     6001 cgcattccgc gtctcgccag gcgatccagg cacgttgcgc cttgtccatc gccgggccga
     6061 aggccttctc cgacggctcg gtgtatcgtt cccgcagggt cttgatcgcg gcggccatct
     6121 cgcggccctg cccctgcgtc ttgcgcgcaa ggcaggcgcc catgtcgcgt tccgttgcgg
     6181 cgttgcggca ctcctcctgc gccagcgctg gcatgacgga caaaagaaac agggcacaaa
     6241 gcccggttga agccttgcgc cctgctccgg tcatggtcgt gctcctaccc cagatggaac
     6301 ttcttcagat cacggtgttc gccgacgata cgacggaccg tgccggtgag cgagcgatag
     6361 atgatggtct cggtctcgat gagttcgcca tagaacttga cgccgcgcag cagcgccccg
     6421 tcggtgacgc cggtggcggc aaccaccacg tcgcccgagg cgagctcgtt gagatcgtac
     6481 ttctttttcg ggtccttgac gcccatcttg gcggcgcggg ccaccttttc cggcgtatcg
     6541 aggatgagac ggccctgcat ctggccgccg acgcagcgca gggcgcctgc cgccagcacg
     6601 ccttccggtg cgccgccaat gccgagatag atgtcgacgc cggtcttttc cggctgcgtc
     6661 gtctggatca cgccaaccac gtcgccgtcc gtgatgagac ggatggaaca gccgatcgtg
     6721 cgcaggcggg cgatcagttc ggcatggcgc ggacggtcca tcaccagcgc cgtcacctcg
     6781 ccgggcttgc agcccttggc cttggccacc gccatgatat tgtcttccgg cgaggcgtcg
     6841 agatcgacga tgcccttggg atagccaggg ccgatcgcaa tcttgtccat gtagacgtca
     6901 ggggcgtaga gcagcgtgcc ggcctgggcc atggccatca cggcgatgga gccgggcatg
     6961 tccttggcgc aaagcgtggt gccctcaagc ggatcgacgg cgatgtcgac cttggggccc
     7021 ttcttcgtgc cgacgcgctc gccaatgaac agcatcggcg cctcgtcgcg ctcgccctcg
     7081 ccgatcacca cctcgccgtc gatcggcagc ttgttgagct cgcgccgcat ggcgtccact
     7141 gccgcctggt ccgcagcctt ttcatcgccg cgcccgcgca ggcgggccga agccaccgcc
     7201 gcgcgctcgg tgacgcgaac cagttccatg gtgaggatgc gctcgatgat gcggtcttca
     7261 gtgacgacga tttcggtcat gtcctggcct gtcgggaatc gttgatgcgg taagcgcgct
     7321 atacagggtt ttgccggccc tgtcagagcc gccggcgcct gcatacgtca ccaaccgcca
     7381 gaaatcccca aaaattgcca caacggagag caaattctgg cgaaatccgt gaaaagcacc
     7441 actgcgatgt taacacgatc ttcgaacctc tgaggtcaac tgctgcctgg taacgcagcg
     7501 gaaaacgatg ttgtcgcgtg gggcacgatg atcaacggca tgctgagggc ggcgacgctg
     7561 ctatttctcc tctcgcctgc gctggcgggc caaccgggcg tggctggcga ctgcgccggc
     7621 atgccctccg tgcagaggcc ttgcgccagc agcgagattg accggctcac ccgggaagtc
     7681 gagacggcgt actccgttgc gctcaacggt atcggcagcg ccggccggag cgctttgaag
     7741 cgcgatcagg agtcgttccg gcgcatcctc gaccagtcat tgagccttgg cgaccaggat
     7801 gccgagacgc tgttgaccct gcgccgcgac cttctggatt ccatccggcc ctcgcgcggg
     7861 gagtggaccg gcagttgggc gaacgcgttc ggaacgatcg aaatcctcgc tacaagtggc
     7921 ggcaactatc acgtgcgcct gcatgccgct gatcctggcg ctggcggctg ggtttgtgaa
     7981 ttcgaggaca gcgccatcct ggcgccgcgc ggcatcgcca tgatcgtcgg cgcggaaagc
     8041 gccgcggatg accatggggg ccccaacgag ggatggaccc tcgcgttccg cctcgctggc
     8101 gacatcctca tggtggaacc gctgagaccg aagggcttcc cgggcgccat gccgttctgc
     8161 ggcaagggcg gcaacgtggc gggcaacttc tttgcccagg atcacggatt ggccttgcgc
     8221 aggacttcgg agcgcaagta gcgccggtca gcgctcgatg cggatgacct gcggatcacc
     8281 ttccacaacc ccgtcgtctg cgatcctggc cagcgccgag cggatggcgg attcgctcgt
     8341 ggcgtaggtg atcagcacaa ccggcacgga ctggcccgag gcggcgcgat ctcggccgcc
     8401 cttctgcagg atgctttcca gcgaaatcga gcgttcagcc atgcgcgtcg cgatagcggc
     8461 ggccgcgccc ggcttgtcct tgacctggag gcggatgtaa tagccgccct cgtgccggcg
     8521 gatcggtgcg cgcaccagct tttccagcgc atccgccggc cgaccgaaaa ccgagaccga
     8581 gatgccgcgg gcgatatcga cgatatcggc gaccaccgcg ctgccggtcg ccccgccccc
     8641 tgcccccggg cc
//
LOCUS       DAOMXN010000100         8399 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_4432_length_8399_cov_10.838926, whole genome shotgun sequence.
ACCESSION   DAOMXN010000100 DAOMXN010000000
VERSION     DAOMXN010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 8399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8399
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4432_length_8399_cov_10.838926"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1804)
                     /gene="purL"
                     /locus_tag="PK812_08260"
     CDS             complement(<1..1804)
                     /gene="purL"
                     /locus_tag="PK812_08260"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HRJ69586.1"
                     /translation="MIPNEPKITPELVAQHGLKPDEYQRFLDLMGREPTITELGIVSA
                     MWNEHCSYKSSKVHLKTLPTKGPRVIHGPGENAGVVDIGDGDVVVFKMESHNHPSYIE
                     PYQGAATGVGGILRDVFTMGARPIAALNSLRFGAPGHPKTRHLVAGVVAGIGGYGNSF
                     GVPTVAGAVGFHERYNGNCLVNAMAVGIARKDLIFTSQATGVGLTIVYLGSKTGRDGI
                     HGATMASAEFDEKSEEKRPTVQVGDPFAEKLLLEACLEIMAKGCVVAIQDMGAAGLTC
                     SAVEMGAKGDLGVELDLDSVPCRESGMTAYEMMLSESQERMLMVLHPHKEAEARAIFT
                     KWGLDFAVVGKTTDTLRFVIRHRGEVKANMPIKELGDQAPEYNRPWVTPAKRPVLAET
                     LVEQPMLATEALLKLLSSPEFCSKRWVFEQYDHLILGNTVQRPGGDAAVVRIGDGPKG
                     LALATDVTPRYVEADPFEGGKQAVAECWRNLTATGALPIALTDNLNFGNPEKPEIMGQ
                     FALAVRGIGEAARALDFPVVSGNVSLYNETQGRAILPTPTIGGVGLVPDVAKAAGIAF
                     RAAGETILLVGETTGWLGASAYLQIVLGREEGAPP"
     gene            complement(1875..2513)
                     /locus_tag="PK812_08265"
     CDS             complement(1875..2513)
                     /locus_tag="PK812_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HRJ69587.1"
                     /translation="MRIIAHQDDIAEGMDALIRRDHRLRPVAEMAGLPPLRRSEGGFS
                     GLCGIIVAQQLSVASADAIWGRVRTRLSPLTPRTVLQAEEPDFRACGLSKPKIRTLRA
                     IAEAVDSGLLEIETLHELPADAAHANLTQVKGIGPWTADIYLMFCLGHADAFAAGDLA
                     LQEAVRLAFGLENRPSASELAETAEIWRPWRAVAARLLWAYYRVAKGREGIA"
     gene            2553..3416
                     /gene="gluQRS"
                     /locus_tag="PK812_08270"
     CDS             2553..3416
                     /gene="gluQRS"
                     /locus_tag="PK812_08270"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HRJ69588.1"
                     /translation="MQPVFRFAPSPNGYLHLGHAYSALVNQRHARETDGRLLLRIEDI
                     DVVRCRPELVEACLEDLHWLGLTWEEPVLRQSTHFPRYRAVLETLKERRLLYPCFCSR
                     KDIVAHVATEEARTGAAWPRDPDGTPRYPGTCRSRPAAEIAKKVAAGEAFALRLDMGA
                     ALSASGMRGLSWREVGAGRVVADPALWGDVVLGRKETPTSYHLAVVLDDALQGVTDVV
                     RGMDLYAATAVHRVLQTLLGLPEPRYRHHTLIRDPTGQKLAKSRASTPLRQLRAEGWS
                     AADVRRALGVD"
     gene            complement(3419..4342)
                     /locus_tag="PK812_08275"
     CDS             complement(3419..4342)
                     /locus_tag="PK812_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HRJ69589.1"
                     /translation="MLAAFLTVLPIFLVIALGWAATRLRLIGEKTGEGLSDYVFVIAI
                     PALLFRSLVSAKWPDIQPWSFWASYFGALALVWAAASLVARRAFGRSAEEAVVAGIAT
                     GYANTVLIGIPLILRVFGEEGAVPLFLLIAVHTPVTIAVATLLVESAGGAGGKAVSIA
                     RKLARNPIILAILAGMAWRAFGIPLPDSAMATIKFIGDSAAPCALFAMGTALSRYGLG
                     GEPRLLALIVALKLVVHPLAVWLLASQVFGLPPLWTAVATVLASCPAGVNAYLFAERH
                     RVGMALASGAIAVTTVISIASISFWIAFATR"
     gene            complement(4580..5176)
                     /locus_tag="PK812_08280"
     CDS             complement(4580..5176)
                     /locus_tag="PK812_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69590.1"
                     /translation="MQAHEIGDDRQSETGSGGAVVQGDTPAEYSFRLGRRQSGTFIAE
                     FEACMGFPATIDGRNHGKKYFAAPGQPKRIVHQIFQNLPQMPYIRDQRLGANRRHVEP
                     KPGAADERTRLAGNDKRVKARTDRAAMITGRRVIARGLDQVIGNRAQMYRRQPKSLHK
                     AVLVRIQFGRVEQVRHRFDTAQRGSQFVGDVSDLRGGK"
     gene            5243..5716
                     /locus_tag="PK812_08285"
     CDS             5243..5716
                     /locus_tag="PK812_08285"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRJ69591.1"
                     /translation="MFEHGNCAPFGKLPEKETAGPPSHRHVLVIDDHASSLQVLATIL
                     DHLGFEVVSTNSPLRGLEMAGAERYGAIFTDMTMPELDGWALVDRLRALPAPFGTCPI
                     FGVSGRVTDDEKARFHAAGGTAFVEKPIGIASLKRALGQNGFLLPVRSARLHAHA"
     gene            complement(5720..7696)
                     /locus_tag="PK812_08290"
     CDS             complement(5720..7696)
                     /locus_tag="PK812_08290"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRJ69592.1"
                     /translation="MPRAASSLTIDLGFEPPLAVSGQSSEAPARRRVNGRWLAAIVLT
                     GLAGMALMFAAATSTMEHRGSEVHQPQMIKPREHASQLAGELTIAARRADRLVQRADI
                     IAARQTFKLPVTRRGPGGDVIRAASFTRIASPLALESLGFADAVPSFNVARLIADASE
                     DRALEDAPPGNLDADVALQNRSLDDWAGGPQAPNLLGDDESRSQVQEALTAARRTAPT
                     LPAQMLLAKAMRSPSELAAVNSFAAPGKEPFSRLVVRMVPENVTALPRLEAALARNQI
                     EQRAVTIRPDENAETLFRNLGVSPSQTRDILRNLVRGGQQLDVGRRLRLVFQALAPGQ
                     AKALVRVDLYNEEARIGSVGRRDDGVFVPIESPRAAPEVRRAGDDDSEQEGGFTLFQS
                     LHETARKHAIPKPMIDEMVRVLFFDVDLQRGVRGGDSFEILIGNDEAEQPTELMMLSL
                     SIGGEVRKFYRFPLPDDEVVDYFDDQGRSAKKFLIRKPVTEGELRSTFGNRRHPILGY
                     WRMHNGVDWAARNGTPIMAAGNGTVRLADWDSGYGRRVEIVHTNGYVTTYSHLSAFGR
                     GIKEGARVRQGQVIGYIGSSGLSTGPHLHYEVMINERYVDPLAIKLPRGRELEGAALA
                     EFKRMREQTDSILKRAPGSAASLATAPPPQTARP"
BASE COUNT         1414 a         2696 c         2787 g         1502 t
ORIGIN      
        1 gcgggggagc accttcctcg cgtcccagca cgatctgcag gtaggcggag gcgccgagcc
       61 agcctgtcgt ttcgccgacc agcaggatgg tctcgccggc ggccctgaag gcgatgccgg
      121 cggctttcgc cacgtccggc accaggccga cgccgccgat ggtgggggtc ggcaggatcg
      181 cgcggccctg cgtctcgttg tagagcgaga cgttgcccga cacgacgggg aagtcgagcg
      241 cgcgcgccgc ctcgccaatg ccacggaccg ccagcgcgaa ctggcccatg atctccggct
      301 tttccggatt gccgaagttc aggttgtcgg tgagcgcgat cggaagggcg ccggtggcgg
      361 taaggttgcg ccagcattcg gcgaccgcct gcttgccgcc ctcgaacggg tcggcctcga
      421 catagcgggg ggtgacgtcg gtcgccagcg cgagcccctt tggaccgtcg ccgatacgca
      481 cgaccgcggc gtcgccgccg gggcgctgca cggtgtttcc gaggatcagg tgatcgtatt
      541 gttcgaacac ccagcgcttg gaacagaact cgggcgagga cagcagtttc agcagcgctt
      601 cggtggcgag catcggctgt tctacgaggg tttcggccag gaccgggcgc ttggcgggcg
      661 tcacccaggg ccggttgtat tccggcgcct gatcgccgag ttccttgatc ggcatgttcg
      721 ccttcacctc gccccggtgc ctgatgacga agcgcaaggt gtcggtggtc ttgcccacga
      781 cggcgaaatc caggccccat ttggtgaaga tcgcccgggc ttccgcctcc ttgtgcgggt
      841 gcagcaccat gagcatgcgc tcctggcttt ccgacagcat catctcgtag gcggtcatgc
      901 cggattcgcg gcaaggcact gaatccagat ccagttccac gccgagatca cccttggcgc
      961 ccatctcgac ggccgagcag gtcaggccgg cggcgcccat gtcctggatg gcgacgacgc
     1021 agcccttcgc catgatttcc aggcaggctt ccagcagcag tttttcggcg aaagggtcgc
     1081 caacttgcac ggttggccgc ttttcttccg atttttcatc gaactcggcg ctggccatgg
     1141 tcgcgccgtg aatgccgtcg cgccctgtct tggagccgag gtagacgatc gttagtccga
     1201 cgcccgtcgc ctgcgaggtg aagatcaggt ctttccgggc aataccgacg gccatggcgt
     1261 tgaccaggca gttgccgttg tagcgttcgt gaaagccgac ggcaccggcg acggtgggaa
     1321 cgccgaacga attgccgtag ccgccgatgc cagcgacgac gccggcgacg aggtggcggg
     1381 tcttgggatg cccgggggca ccgaagcgca gggaattcag cgccgcgatc ggccgggcgc
     1441 ccatggtgaa gacgtcccgc aggatgccgc cgacgcctgt cgccgccccc tgatacggct
     1501 cgatatacga gggatggttg tgcgattcca tcttgaaaac aacgacatcc ccatcgccaa
     1561 tgtcgacgac gccggcgttc tcgccggggc cgtggatcac gcgcggaccc ttggtcggca
     1621 aggtcttgag gtggaccttg gaggacttgt aggagcagtg ctcgttccac atcgccgaga
     1681 cgatgccgag ctcggtgatc gtcggctcgc ggcccatcag gtcgagaaag cgctgatact
     1741 cgtccggctt cagcccgtgc tgggcgacga gttcgggcgt gatcttgggt tcgttcggga
     1801 tcatggcgtc atcgctgcga aaagtgggcc ggtcatagtc ggggacgccg gattccgcca
     1861 ctgattcttg cggctcaggc gattccctcg cgccctttcg ccacgcgata gtaggcccag
     1921 agcagccgcg ccgcgacggc ccgccagggt ctccagatct ccgccgtctc ggccagttcg
     1981 gaggccgatg gccggttctc aagcccgaaa gcgagccgca cggcctcctg aagggccaga
     2041 tcgccggcag cgaaggcgtc ggcatgccca agacagaaca tcaggtagat gtcggccgtc
     2101 caggggccga tccccttcac ctgcgtcaaa ttggcgtggg ccgcgtcagc gggcagttcg
     2161 tgcagggttt cgatttcgag caggcccgaa tcgaccgctt cggcgatcgc gcgcagcgtg
     2221 cgaattttcg gcttcgacag gccgcaggcc cgaaaatccg gctcttcggc ctgcagaacg
     2281 gtgcgcggcg tcagcggtga caggcgtgtc cgcacgcgcc cccagatggc atcggcgctg
     2341 gcgaccgaca gctgttgcgc cacgatgatg ccgcacaggc cggaaaaccc gccctcgctg
     2401 cgtcgcaggg gcggcaggcc ggccatttcc gcaaccggcc gcaggcggtg atcgcgccgg
     2461 atcaacgcgt ccatcccttc cgcgatgtcg tcctggtgtg cgatgatgcg catctgatcc
     2521 ccccgcacca gttgacgcgc agggcaaggc cattgcaacc cgttttccgc ttcgcgccca
     2581 gtcctaacgg ctacctgcat cttggccatg cctattccgc tctggtgaac cagcggcatg
     2641 cccgcgagac cgacggtcgc ctgttgctgc ggatcgaaga catcgatgtc gtgcgctgcc
     2701 ggccggagct ggtcgaggcc tgcctcgagg acctgcactg gctcggactc acctgggaag
     2761 agccggttct ccggcagtcg acgcactttc cgcgctatcg cgccgttctc gaaaccctga
     2821 aggaacggcg gctgctctat ccgtgttttt gcagccgaaa ggacatcgtc gcccacgtgg
     2881 cgaccgaaga ggcgcgtacc ggcgccgcct ggccccgcga tccggacggc acgcctcggt
     2941 atcctggaac ctgccggagc cgcccggcgg cggagatcgc gaaaaaggtg gcggcgggcg
     3001 aagctttcgc cctgcgtctc gacatggggg ccgcgctgtc agcgagcggg atgcgcggcc
     3061 tttcctggcg ggaggtgggc gcagggcgcg tggtcgccga tcccgcgctc tggggcgatg
     3121 tcgtgctcgg gcgcaaggag acgcccacga gctatcatct tgccgtagtg ctcgacgatg
     3181 cgttgcaggg ggtcacggat gtggtgcggg gcatggatct ttatgccgcc acggcggtac
     3241 accgggttct gcaaaccctg cttgggctcc ccgagccgcg ctacaggcac cataccctga
     3301 tacgcgaccc gaccggtcaa aagctcgcca agagccgggc ctccactccc ttgcgccagt
     3361 tgcgcgccga gggctggagc gccgcagacg tccgtcgtgc gttgggggtc gactgacgtt
     3421 accgcgttgc gaaagcgatc cagaagctga tgctggcgat tgaaatcacg gtcgtgactg
     3481 cgatggcgcc ggaggcgagc gccatgccga ccctgtgtcg ttcggcgaac agataggcat
     3541 tcacgccggc cggacaggac gccagcaccg tggcgaccgc cgtccagagc ggtggcaggc
     3601 cgaacacctg cgatgccagg agccaaaccg ccagcggatg cacgacaagc ttcaacgcga
     3661 cgatcagcgc cagcaggcgc ggctcgccgc cgagcccgta gcggctcagg gccgtcccca
     3721 tcgcaaacag ggcgcaggga gccgccgaat ccccgatgaa cttgatcgtc gccatggccg
     3781 aatcgggcag gggaattccg aaggcgcgcc aggccatgcc ggccaggata gcgaggatga
     3841 tcggattgcg ggccagcttc cgagcgatgc tgaccgcctt gccgccggcc ccgccagcgc
     3901 tttccaccag cagcgtggcg acggcaatgg tcacgggcgt gtgcacggcg atcagcagga
     3961 acaggggaac cgctccttcc tcgccgaaaa cgcgcaggat gagcggaatg ccgatcagca
     4021 cggtgttggc atagcccgtg gcgatgccgg caaccacggc ttcctcggcc gagcggccga
     4081 aggctcgccg ggcgaccagg ctcgccgctg cccagaccag ggccagcgcg ccgaaatacg
     4141 atgcccagaa cgaccagggc tggatgtcag gccatttcgc gctgaccagc gaccggaaca
     4201 gcagcgcggg aatggcaatg acgaagacat agtccgaaag tccctcgccg gtcttctcgc
     4261 cgatgagcct gaggcgcgtc gccgcccagc ccagcgcaat gaccagaaaa atcggcaaga
     4321 cggtgaggaa cgcagcaagc atcgggatcc tggcgtggtg gggcgaaaac tcaagaaatt
     4381 gccttgcctt atgagtttcc actcaagggc cgccggcatc ttaccgttcg gcttaggagg
     4441 cgcttaaggt gtttttccat ataaccaggc cgggcagggt cgacgcaatc gctggcatga
     4501 tctgattcct catggcggcc ttgggcggga gcattctttg actgaatcgg acaagattcc
     4561 ggccgagacc ggcgttcgcc tactttccgc cgcgcagatc gctgacatcg cccacgaact
     4621 gcgatccccg ctgggcggta tcgaagcgat ggcggacctg ctcgacgcga ccgaactgga
     4681 tgcgaaccag aaccgccttg tgcaggctct taggctggcg gcggtacatc tgcgctcggt
     4741 tgccaatgac ctgatcaagt ccccgggcga tcacacgccg gccggtgatc atcgcggcgc
     4801 gatcggtgcg tgccttgacg cgtttgtcgt tgccagcgag gcgcgtgcgc tcgtccgcgg
     4861 cgcccggttt cggctcgaca tgccgccggt ttgcgcccag gcgctggtcg cggatgtacg
     4921 gcatctgcgg caaattctgg aaaatctggt ggacaatgcg cttcggttgt ccgggcgcgg
     4981 cgaagtactt ctttccgtgg ttccggccgt cgatggtcgc ggggaacccc atgcacgctt
     5041 cgaactccgc gatgaaggtc ccggactgac gcctgcccag gcggaacgaa tattcggcag
     5101 gggtgtcacc ctggacgacc gcgccgccgg agccggtctc ggactgtcga tcgtcgccga
     5161 tctcgtgcgc ctgcacggcg gcacatgtgg tgctttcggc cgtgatgacc gccagggggc
     5221 atgcctctgg ttcacgtttc ccgtgttcga acacggaaat tgtgctccgt tcggcaagtt
     5281 gccggagaag gaaaccgccg gcccgccctc gcaccggcat gtgctggtca tcgacgacca
     5341 tgcctccagt ctgcaggtcc tggcgaccat cctcgatcat ctcggcttcg aagtggtctc
     5401 gaccaattcg ccgctgcgcg ggctcgaaat ggctggcgcc gagcgctatg gcgccatctt
     5461 caccgacatg accatgccag aactggacgg ctgggcgctc gttgatcggt tgcgggccct
     5521 gccggcgccg ttcgggacct gtccgatttt cggtgtcagc gggcgcgtca ctgacgacga
     5581 gaaagcccgg tttcacgccg ccggcggcac ggccttcgtg gaaaagccga tcggcatcgc
     5641 gtctctcaag cgagcgctgg gccagaacgg gttcctcttg ccggttcggt cggcgcggct
     5701 tcacgcccac gcctgagggt catggtcgag cggtctgcgg cggcggtgct gtggcaaggc
     5761 tggcagcgct gccgggcgcg cgcttcagga ttgaatcggt ctgttcacgc atgcgtttga
     5821 actcggcaag cgccgcgcct tcaagctccc ggcctctcgg gagcttgatg gcgagcgggt
     5881 cgacgtagcg ctcgttgatc atcacttcgt agtgaaggtg tggtccggtc gacaggccgg
     5941 agcttccgat gtagccgatg acctgcccct ggcgcacccg cgcgccctcc ttgattccgc
     6001 gaccgaacgc agacaggtgg ctataggtgg tgacgtatcc gttcgtgtgg acgatctcga
     6061 cgcggcggcc atagccggaa tcccagtcgg caaggcggac ggtgccgttg cctgcggcca
     6121 tgatcggcgt tccgttcctg gctgcccagt cgacgccatt gtgcatgcgc cagtagccca
     6181 gaatcggatg gcgacggtta ccgaaggtcg accgcaattc gccctcggta acgggcttgc
     6241 gaatcaggaa tttcttcgcg gaacggcctt gatcgtcgaa ataatccacc acttcgtcat
     6301 cgggcagcgg gaagcggtag aacttgcgga cctcgccgcc gatgctgagg ctcagcatca
     6361 tcagttccgt gggctgttcg gcttcgtcgt tgccgataag gatctcgaag gaatcgcccc
     6421 ctcgcacgcc gcgctgcaga tcgacatcga agaacaggac acgcaccatc tcgtcgatca
     6481 tcggcttggg aatcgcgtgc ttgcgcgccg tctcgtgcag gctctggaac agggtgaagc
     6541 cgccctcctg ttcgctgtcg tcgtcgcctg cgcggcgcac ttccggcgcg gcccgcggcg
     6601 attcgatcgg cacgaaaaca ccgtcgtcgc ggcgccccac cgatccgatc cgcgcttcct
     6661 cgttgtagag gtccacgcgg accagtgcct tggcctggcc aggggcgagc gcttggaaaa
     6721 ccagcctaag gcgccggccg acatcgagtt gctgtccgcc cctgaccaga ttgcgcagga
     6781 tgtcgcgcgt ctgagatggg ctaacgccga gattgcgaaa cagcgtctct gcattttcgt
     6841 cggggcggat ggtgacggcg cgctgctcga tctggttgcg cgccaaggct gcctcgagcc
     6901 tgggaagcgc cgtcacattc tccggcacca tccgcacgac aaggcgcgaa aatggctcct
     6961 tgccgggcgc ggcaaacgaa ttcacggccg caagttccga agggctgcgc atggccttgg
     7021 ccagaagcat ctgcgccggc agggtcggag ccgtgcggcg ggcggctgtc agggcctcct
     7081 gaacctgtga tcgcgattcg tcgtcgccga gtagattcgg tgcctgggga ccgcctgccc
     7141 aatcgtcgag actgcggttc tgcagcgcca cgtccgcgtc gaggtttcca ggcggcgcat
     7201 cctccagggc tcgatcctcg ctggcgtcgg cgatcagccg cgccacgtta aaggatggca
     7261 cggcatcggc gaatccgagc gattccagcg ccaaagggct cgcgatgcgg gtaaagctcg
     7321 ccgcgcgaat cacatcgcct ccagggccgc ggcgcgtgac cggcagcttg aaggtctggc
     7381 gcgccgcgat gatgtccgca cgctgaacca ggcgatcggc cctgcgcgcc gcgatggtca
     7441 attcgccagc gagctgggag gcatgttcgc gcggcttgat catctgaggc tgatgaacct
     7501 cgctgccgcg atgctccatc gtgctggtcg cggcggcgaa catcagcgcc atgccggcaa
     7561 ggcctgtcag tacgatcgcc gcaagccacc gaccgttgac gcgccgccgc gccggcgctt
     7621 cggaggattg tccggagaca gccagcgggg gctcgaagcc aagatcgata gtcaggctgg
     7681 atgcggctct aggcatcagg acgtggttcg ttcccaggtt gctataaaag gctcagccaa
     7741 atcgcgcgcc ctgtatagcc cgagggcgcg cgatgcccaa gccgtcccaa ccgctttcgg
     7801 tcgttgcccg cgactgcgcc cggctcctgc ggcaaagtta tggccgcttg cggcgtgcgg
     7861 aatcgtggat tggcgcaaag aattttcggt gtcgaccgct ggcgcttgag ccattcacct
     7921 agccctccgc cgccagctta ccgaaaagcc gcccctcgtg gcccattttc gatgattttc
     7981 gcgctccggt gtgcgctgca gaaaaaaatt caaaatcggc gttgacagcc ggaaacgggt
     8041 ggcgctataa gccgcccatc gacgcgctgc ttcggcggcg cgggcctcgc ttcctctgaa
     8101 agacgagttg gtcttttgac ccggctccgc tgggatagga tagtttggct tcgctgtttg
     8161 gcaattgaag acgagaaaga gaaacgtgga cggcgaggtt cttgcggacc gatcctaacc
     8221 gatcggtcgg aaagagactt cggcggtcta acgtttcggt gagtgtacac acgcttggtc
     8281 ccctcgggga tcagcagtgc actccgtcaa tgagacgttg tgatcagccg gttcaaaatc
     8341 tcaatccaac ttgagagttt gatcctggct cagaacgaac gctggcggca ggcttaaca
//
LOCUS       DAOMXN010000101         8339 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_4470_length_8339_cov_12.267383, whole genome shotgun sequence.
ACCESSION   DAOMXN010000101 DAOMXN010000000
VERSION     DAOMXN010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 8339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8339
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4470_length_8339_cov_12.267383"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="PK812_08295"
     CDS             complement(<1..869)
                     /locus_tag="PK812_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009492536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ69593.1"
                     /translation="MPDGGKSETPPMLAVENLSLSFDALDLPATLVDRVSFEVRPGRT
                     LCLVGESGCGKSVVSLAVMGLLPRPPARITGGRILLDGENLLDKSPAALSDLRGRVMA
                     MIFQEPMTSLNPAFTVADQIVEAIRRHNDIPAREARARALAMLRKVRIPAPEERMNAY
                     PHQLSGGMRQRVMIAMALANAPRLLLADEPTTALDVTIQAQILRLIRDLQREAGTAVV
                     LVTHDLGVVAEVADDVAVMYAGRIVEQGSVKAIFDDPQHPYTIGLMGSLPSLGTRQGR
                     LAAIPGAVPPPSR"
     gene            1051..>1736
                     /gene="dctP"
                     /locus_tag="PK812_08300"
     CDS             1051..>1736
                     /gene="dctP"
                     /locus_tag="PK812_08300"
                     /inference="COORDINATES: protein motif:HMM:NF037995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HRJ69594.1"
                     /translation="MISKRILGAMALAGAAALALPAQAQTKLKFAHVYEVNEPYHTQA
                     LWAAEEIKKRTNGKFEVQVFPASQLGNEPQINEGLGLGTVDIIYTGAAFAGNTYKPLA
                     ISNAPFIFRDYAHWQAYRTSPLFRELAKGYEDITKHKVIVMTYYGERMVTANKEIKKP
                     DDMKGMKLRVPQAPLYIMFARSVGANATPIAFAEVYLALQQKTVDGQENPLPTIMAKK
                     FHEVQSHIMLT"
     assembly_gap    1737..1836
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1837..2186
                     /locus_tag="PK812_08305"
     CDS             <1837..2186
                     /locus_tag="PK812_08305"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRJ69595.1"
                     /translation="PTIMAKKFHEVQSHIMLTSHISDTLVTIVGNHLWNRLNDAEKKV
                     FADVWAEAAQKASDDIRKAELALVDEFRKLGKNVVTVDRAAFQAAALPLLNDASAGAG
                     WTKAHFDALQALK"
     gene            complement(2248..3354)
                     /locus_tag="PK812_08310"
     CDS             complement(2248..3354)
                     /locus_tag="PK812_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007820507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HRJ69596.1"
                     /translation="MAKIVRIEILQVDLTPKVKRTDAIQSFVTQETPMVRIHTSDGRI
                     GTGYTYTIGTGGSSVIGLLRDHLCPRLIGRDPLDIEALWKDLFFATHATAVGAITALA
                     LAAIDTALWDLKCLASGRPLWKEAGGAQASIPLYTTEGGWLHIDTPALVEDALKARAD
                     GFGGSKIKVGRPRASEDVARLAAVREAVGPGFEIMVDANQAFTVSEAIRRARLYEPFD
                     LAWFEEPLPAEDLGGHIRLSAATTLPVAVGESIYHPSHFREYLQRGACSIVQADVARI
                     GGITPWLKVAHLAESFNVAICPHFLMELHVSLCAAVPNAAWVEWIPQLDDVTTSRMTV
                     RNGRAAPPDGPGLGIAWDWKAIGARQAFAPIVIS"
     gene            3456..4013
                     /locus_tag="PK812_08315"
     CDS             3456..4013
                     /locus_tag="PK812_08315"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRJ69597.1"
                     /translation="MTGAGKDSGAGEEHLIVVEEQEIRNEYFPEDVVAFVLFWAMGGI
                     VFLQFFTRYVLNDSVAWTEEIARYLLMWVTFVGAAVAMRRRVHIAVEVLHNFLPPGPV
                     RVLNFVIDVITTGFVGLLCWYALTIYERMKIQKMTVIDWPMSYVYAGVGLGCFLLLYR
                     TVQMVFSNARRGWRPDPARQGLILD"
     gene            4026..5510
                     /locus_tag="PK812_08320"
     CDS             4026..5510
                     /locus_tag="PK812_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006725363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRJ69598.1"
                     /translation="MAVLFFSFFILLFVGVPIFIALAGSSLAYIWAHTFIPDFVVLHR
                     MAGGVDSFPLLAVPYFILAGNLMNTAGITNRIYDFAVALVGWLKGGLGHVNVIGSVIF
                     SGMSGTAVADAGGLGTIEIKAMRDHGYKDEFAVGITAASATIGPIIPPSLPMVIFGVM
                     ANVSIGQLFAAGIVPGLMMGGFLMTYITYYAHMHGVGRDQRFRLIVLGRTFVRAVPAL
                     MTPAIIIGGMSFGIFTPTEAAIAACFWALFLGLIFYPLSSQLEGMGRGIGGALLVMTG
                     MVAAAWAFAQTGVVSEVRAVITAITFNLVACVAAYHYLRRARGVQPAYKPMTFAQFYK
                     ISLETVETTASVLIIVGAASLFGWVLTTTRVTDTIAEGLLSITTNPLMILLIINAFLL
                     VVGCFMETIAAISILVPVFMPIITKVGIDPVQFGVIMVLNLMIGLLTPPVGMVLFILS
                     RVASISFERTVKAVIPFLIPLLAVLALISIFPQLTLYLPTLWYR"
     gene            5642..6817
                     /locus_tag="PK812_08325"
     CDS             5642..6817
                     /locus_tag="PK812_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HRJ69599.1"
                     /translation="MPVHNRIAEFSADMAAWRQDLHAHPELGFEEHRTSAIVADKLAS
                     WGIEVHRGIAGTGVVGVLRGTGSSSRSIGIRADMDALAMPEFNEFAHRSRHPGKMHAC
                     GHDGHTTMLLGAARYLAETRNFDGTVVFIFQPAEEGGGGGRVMVEDGLFERFPCDSVY
                     AAHNDPLLPAGTITAVPGPVMAAADMLEIAIRGRGGHAARPHHTVDPVLIGSHIIMAL
                     QSIVARKVDPLDSAVVGITTFHAGSAKNVIPETAELTGTVRTFRPQTQDEVEQLISRI
                     VTSTAALHGGSAELRYERGYPPVVNSAEHTARAGRAAEKVVGAALVKRDHPPTMGGED
                     FSFMLLRRPGCFVRIGQKGKDKGGVPLHNSSYDFNDEIAPVGASFFSTLVEQELPRG"
     gene            6874..>8339
                     /locus_tag="PK812_08330"
     CDS             6874..>8339
                     /locus_tag="PK812_08330"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRJ69600.1"
                     /translation="MTQTSTSGPVQVFPQSADDALGLDRFAVGQSVSRAEDPVLLRGE
                     GRYSDDVSLPGQVHAAFVRAREAHGVIRDIRTARARAMKGVLLVITQADLDAAGFGEI
                     RTKVPIRQPDGRTPPSRPPLARGKVRFSGEIVACVVARTLDQALDAAEAVELDIDALP
                     AVTLASEADAPGAPQLFESVPGNVIYDYRYGDEAAVAAAFERAHHVTRLGLRNTRLVV
                     NPLEPRSALCSYDAREKRYTLRAACQGVKGLQANIASDLLQAPASAMRVLTGQIGGSF
                     GLKGAVFPEYACLLHACRRLRRPVKWTDRRSDSFLGDYHGRDDERLGELALDRRGRIL
                     ALRISGHGNLGAYVTAVGTMASTSNVPKNAAGVYATPVMAVHSRTMVTNTVPIGPYRG
                     AGRPEANYFMERLMDVAAAETGIDRITLRRRNHIRPSMLPFTAASGLVYDSGDFPAVL
                     DRALEASDWKGFGKRKAASRRRGLLRGIGLGQFLEGTAA"
BASE COUNT         1322 a         2713 c         2775 g         1429 t
ORIGIN      
        1 cagcgcgagg gcgggggcac ggcgccggga atggcagcga gccggccctg ccgcgtgccg
       61 agcgagggca gcgaccccat caggccgatg gtgtagggat gctgcggatc gtcgaagatg
      121 gccttcacgc tgccctgctc gacgatgcgg ccggcgtaca tcaccgccac gtcgtccgcg
      181 acctccgcga ccacgccgag atcgtgcgtc accagcacca cggccgtgcc cgcttcgcgc
      241 tggaggtcgc ggatgaggcg caggatctgc gcctggatcg tcacgtcgag cgcggttgtc
      301 ggctcgtcgg cgagcagcag tcgaggggcg ttggccagcg ccatggcgat catcacacgc
      361 tggcgcatgc cgcccgaaag ctgatgcggg taggcgttca tccgctcttc gggcgcagga
      421 atgcgcacct tgcgcagcat ggcgagggcg cgcgcgcgtg cctcgcgcgc aggaatgtcg
      481 ttgtggcggc ggatggcttc gacgatctgg tccgccacgg tgaaggccgg attgagcgag
      541 gtcatcggct cctggaagat catcgccatg acgcggccgc gcaggtccga tagcgccgcg
      601 ggcgacttgt cgagcaggtt ctcgccgtcg agcaggatgc gtccgccggt gatgcgcgcg
      661 ggcgggcgcg gcagcaggcc catgaccgcc agggagacga ccgactttcc gcaacccgac
      721 tccccgacga gacacagcgt ccggcccggc ctgacctcga aagagacgcg atcgaccagc
      781 gtcgctggca ggtccagcgc atcgaacgac agggacaggt tctcgaccgc aagcatcggc
      841 ggcgtctcgc tcttgccgcc gtccggcatc acccttgcca cgcacatctc ccgggaccgc
      901 ccgccaggac ggcaatgtcc catggcaggg caggcgcggc acgctgtcaa gcgcggatcg
      961 cgcccgcggg gcgttgaccg gatggcaagt tgtatgacaa gttcacggca agccgagaaa
     1021 cagaccggcg cggtcgcggg aggaaatatc atgatcagca aacgcattct gggcgccatg
     1081 gccctggccg gcgcggctgc cctggcgctg ccggcgcagg cgcagaccaa gctcaagttc
     1141 gcccacgtct acgaggtgaa cgaaccctat cacacgcagg cgctctgggc ggccgaggag
     1201 atcaagaagc gcacgaacgg caagttcgag gtgcaggtct tcccggcctc ccagcttggc
     1261 aacgagccgc agatcaacga gggcctcggc ctcggaacgg tcgacatcat ctacaccggc
     1321 gcggccttcg ccgggaacac ctacaagccg ctggcgatct ccaacgcgcc gttcatcttc
     1381 cgtgactacg cgcactggca ggcctatcgg acctcgccgc tgttccgcga actcgccaag
     1441 ggctacgagg acatcaccaa gcacaaggtg atcgtcatga cctactatgg cgagcgcatg
     1501 gtgacggcga acaaggaaat caagaagccg gacgacatga agggcatgaa gctgcgcgtg
     1561 ccgcaggcgc cgctctacat catgtttgca cggtcggtgg gcgccaacgc cacgccgatc
     1621 gccttcgccg aggtctatct tgcgctccag cagaagacgg tggacgggca ggagaatccg
     1681 ctgccgacca tcatggccaa gaagttccac gaggtgcagt cgcacatcat gctgacnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntgcc gaccatcatg gccaagaagt
     1861 tccacgaggt gcagtcgcac atcatgctga cctcgcacat ctcggatacg ttggtcacca
     1921 tcgtgggcaa ccacctgtgg aaccgcctga acgatgcgga gaagaaggtg ttcgccgatg
     1981 tctgggcgga agccgcgcag aaggcctccg acgacattcg caaggcggag ctggcgctgg
     2041 tcgatgagtt caggaaactc ggcaagaacg tcgtgaccgt cgaccgcgcc gcgttccagg
     2101 ccgccgccct accgctactc aacgacgcct cggccggcgc gggctggacc aaggcgcatt
     2161 tcgatgcgct gcaggcgctg aagtgaggct gctcccccgc gccgcaaccc ggcgcgggga
     2221 tgccgctcga acgaggcggc ggcgctccta gctgatcacg atcggggcga aggcctgccg
     2281 cgcgccgatc gccttccagt cccaggcgat gccgagtccg ggaccatccg gtggcgcggc
     2341 tcgcccgttc ctcacggtca tgcggctcgt cgtgacgtcg tcgagttgcg gaatccactc
     2401 cacccaggcc gcgttcggta cggcggcgca gagcgagaca tgcagctcca tcaggaaatg
     2461 cgggcagatc gcgacattga agctctcggc gagatgggcg accttcagcc agggcgtgat
     2521 gccgccgatg cgtgccacat cggcctgcac gatggagcag gcgccgcgct ggagatattc
     2581 gcggaagtgg ctgggatgat agatcgactc tccgaccgcg accggcaggg tcgtcgcggc
     2641 gctcagccgg atgtgcccgc cgagatcctc ggccggcagc ggttcctcga accaggccag
     2701 gtcgaacggt tcgtagaggc gggcgcggcg gatggcctcg gagacggtga aggcctggtt
     2761 ggcgtcgacc atgatctcga aaccggggcc taccgcctcg cgcacggcgg ccagccgcgc
     2821 cacatcctcg ctggcgcgcg ggcggccgac cttgatcttg gagccgccga aaccgtccgc
     2881 cctggccttc agcgcatcct cgaccagcgc cggcgtgtcg atgtgcagcc agccgccttc
     2941 cgtggtgtaa agcggtatcg aggcctgggc gccgccagcc tccttccaca gcggccgacc
     3001 cgaggcgagg cacttgaggt cccacagcgc ggtgtcgatc gccgccagcg ccagcgcggt
     3061 gatcgcgccg acggcggtgg cgtgggtggc gaagaacagg tccttccaga gcgcctcgat
     3121 gtcgagcggg tcgcggccga tcagcctcgg gcacaggtgg tcccggagca ggccgatgac
     3181 ggacgagccg ccggtgccga tggtgtaggt gtaacccgtg ccgatgcgtc cgtcgctcgt
     3241 atggatgcgc accatcggcg tttcctgcgt gacgaaggac tggatggcgt cggtgcgttt
     3301 caccttcggc gtgaggtcga cctgcaggat ctcgatacgg acgatttttg ccatggcgct
     3361 tcctccggcg gttctggggg catgcccgtc agcctgaggc gcaatccgcc ggcatgcaat
     3421 ggccgaattt cattttgtca ggcgggagcc acgacatgac aggggcaggc aaggacagcg
     3481 gcgcgggcga ggaacacctc atcgtcgtcg aggagcagga aatccggaac gagtatttcc
     3541 cggaggatgt ggtcgccttc gtcctgttct gggcgatggg cggtatcgtc ttcctgcaat
     3601 tcttcacgcg ctacgtgttg aacgattccg tcgcctggac agaggagatc gcgcgttatc
     3661 tcctgatgtg ggtcacgttc gtcggcgcgg cggtggcgat gcgccggcgc gtacacatcg
     3721 ccgtggaggt attgcacaac tttctgccgc ccggtccggt gcgtgtgctg aacttcgtca
     3781 ttgatgtgat caccaccggc ttcgtcggcc tgctctgctg gtatgccctg accatctacg
     3841 agcgcatgaa gattcagaag atgacggtca tcgactggcc gatgtcctat gtctacgcag
     3901 gcgtcggcct cggctgcttc ctgctgctct accgtacggt gcagatggtg ttttcgaatg
     3961 cgaggcgtgg ctggcgaccg gacccggcgc gccagggcct cattctggat tgagcgagaa
     4021 aacccatggc cgtcctgttc ttctcgttct tcattctgct gttcgtcggc gtgccgatct
     4081 tcatcgcact ggccggctcc tcgctcgcct atatctgggc ccacaccttc atcccggact
     4141 tcgtggtgct gcaccgcatg gcgggcggcg ttgattcctt tccgctgctg gccgtgccgt
     4201 atttcatcct cgccgggaac ctgatgaaca cggccggcat caccaaccgc atctatgatt
     4261 tcgcggtcgc gctggttggc tggctcaagg gcggcctcgg gcacgtcaac gtcatcggct
     4321 cggtgatctt ctcgggcatg tccggcacgg cggtcgccga tgccggcggg ctgggaacca
     4381 tcgagatcaa ggcgatgcgc gaccacggct acaaggacga gttcgccgtg ggcatcacgg
     4441 cggcctccgc cacaatcggg ccgatcattc cgccgtcgct gccgatggtg atcttcggcg
     4501 tgatggcgaa tgtctccatc ggacagctct tcgccgccgg gatcgtgccg gggctgatga
     4561 tgggcggctt cctgatgacc tacatcacct attacgccca catgcacggc gtcggccgcg
     4621 accagaggtt ccggctgatc gtgctgggcc gcacctttgt gcgggctgtt ccggcgctga
     4681 tgacgccggc gatcatcatc ggcggcatgt ccttcggcat cttcacgccg accgaggcag
     4741 ctatcgcggc ctgtttctgg gcgctgttcc ttggactgat cttctatccg ctgtcgagcc
     4801 agctcgaagg catgggccgg ggcatcggcg gcgcgctgct ggtcatgacg ggcatggtgg
     4861 cggcggcctg ggccttcgcg cagaccggtg tggtgagcga ggtgcgcgcc gtgatcaccg
     4921 cgatcacctt caacctcgtt gcttgcgtcg cggcctatca ctatctgcgc cgcgcgcgcg
     4981 gcgtgcagcc ggcctacaag ccgatgacct tcgcgcagtt ctacaagatt tcccttgaga
     5041 ccgtggagac cacggccagc gtgctgatca tcgtcggcgc ggcctcgctg ttcggctggg
     5101 ttctgaccac gacccgggtt accgacacca tcgcagaggg cctgctgtcg atcaccacca
     5161 atccgctgat gatcctgctt atcatcaacg cgttcctgct ggtggtcggc tgcttcatgg
     5221 agacgatcgc ggcgatctcg atcctggtgc cggtgttcat gccgatcatc accaaggtgg
     5281 gcatcgatcc ggtgcagttc ggcgtgatca tggtgctcaa cctgatgatc ggtctgttga
     5341 cgccgccggt cggcatggtg ctgttcatcc tgtcacgcgt ggcgagcatc tccttcgagc
     5401 gcacggtgaa ggcggtgatc ccgttcctca tcccgctgct ggcggtgctg gcgctgatct
     5461 cgatcttccc tcagctcacg ctgtacctgc cgacgctgtg gtatcgctag agtatccgtg
     5521 atgatcaggc agtgccctgc ggaccgatgg agctatcctg acgagggcat tgcgcggcgg
     5581 cgccgataga ttagtctcct aagcaaatcg ccgcgcgcga ctcccatcgg atttgcccat
     5641 catgcccgtt cacaaccgca tcgccgagtt ttccgccgac atggccgcct ggcggcagga
     5701 cctgcacgcc catcccgaac tggggttcga ggagcatcgc accagcgcca tcgtcgcgga
     5761 caagcttgcc tcctggggca tcgaggtgca tcgcggcatc gccggcaccg gggtcgtggg
     5821 cgtgctgcgc ggcaccggta gttccagccg ctccatcggc atccgcgccg acatggatgc
     5881 gctcgccatg ccggagttca acgaattcgc gcaccgcagc cggcatccgg gtaagatgca
     5941 cgcctgcggt catgacgggc ataccacgat gctgctcggc gcggcgcgct acctggcgga
     6001 gacgaggaat ttcgatggga ccgtcgtctt catcttccag ccggcggaag agggcggcgg
     6061 cggcggccgg gtgatggtgg aggacgggct tttcgaacgc ttcccctgcg attccgtcta
     6121 tgccgcgcac aacgatccgc tgctgccggc cggaaccatc accgcagtcc caggccccgt
     6181 catggcggcg gcggacatgc tggagatcgc gatccgcggc cggggcggtc atgccgcgcg
     6241 cccgcatcac acggtcgatc ccgttctgat cggcagccat atcatcatgg cgctgcaatc
     6301 gatcgtggcg cgcaaggttg atcccctgga ttcggccgtg gtcggcatca ccacgttcca
     6361 tgccggttcg gccaagaacg tcatcccgga gacggcggag ctgaccggca cggtgcgcac
     6421 cttcaggccg cagacccagg acgaggtgga gcagctgatc agccgcatcg tcacctcgac
     6481 ggcggcgctg cacggcggca gcgcggaact gagatacgag cgcggctatc cgccggtggt
     6541 caattcggcc gagcatacgg cgcgcgcggg gcgtgccgcg gaaaaggttg tcggcgcggc
     6601 gctggtcaag cgcgatcatc ccccgaccat gggcggggaa gatttctcct tcatgttgtt
     6661 gcgccggccc ggttgcttcg tgcgcatcgg ccagaagggc aaggacaagg gcggcgtgcc
     6721 gctgcacaac tcgtcctacg atttcaacga cgagatcgcc ccggtgggcg cgagcttctt
     6781 ctcgacgctg gtcgagcagg agctgccgcg cggctgagtt tcacgctgcc ggcccgccgc
     6841 gcagccgacc gaacctttca ttctggtttt ccgatgacgc agacgagcac atccggccct
     6901 gttcaggttt tcccgcagtc ggcggatgac gcccttgggc tcgaccggtt cgccgtcggc
     6961 cagtctgtgt cgcgcgccga ggacccggtg ctgttgcgcg gcgagggacg ctactccgac
     7021 gatgtgagcc tgcccgggca ggtccatgcc gctttcgtgc gcgcccgcga ggcccatggc
     7081 gtgattcgcg acatccgcac cgcgcgggcg cgtgccatga agggtgtgct gctggtgatc
     7141 acgcaggcgg atctggacgc ggccggcttc ggcgagatcc gcaccaaggt gccgatccgc
     7201 cagcccgatg gcaggacgcc gccatcgcga ccgccgctcg cacgcggcaa ggtacggttc
     7261 agcggcgaga tcgtcgcctg cgtcgtggcc aggaccctgg atcaggcgct ggatgccgcc
     7321 gaggccgttg agctcgacat cgacgccctg ccggcggtga ccctggcctc cgaggcggat
     7381 gcgcccggcg cgccgcagct gttcgagagc gttcccggca atgtgatcta cgactaccgc
     7441 tacggcgacg aggccgccgt cgccgccgcc ttcgagcggg cgcatcatgt gacgcggctc
     7501 ggcctgcgca atacccgcct ggtggtcaat ccgctggagc cgcgctcggc cctgtgcagt
     7561 tacgatgcgc gcgaaaagcg ctacacgctg cgcgccgcct gccagggcgt gaagggcctg
     7621 caagccaata tcgcctcgga cctgttgcag gcgccggcct cggcgatgcg cgtgctcacc
     7681 ggccagatcg gcggatcgtt cggcctcaag ggggcggtct tccccgagta cgcttgcctg
     7741 ctgcatgcct gcagacggct gcggcggccg gtgaaatgga ccgatcgtcg ctcggacagt
     7801 ttcctcggcg attaccacgg gcgcgacgat gagcgcctcg gcgagctggc gctggaccgg
     7861 cgcgggcgca tcctggcact gcgcatcagc gggcacggca atctcggcgc ctacgtgacc
     7921 gcggtcggca cgatggcctc cacctccaac gtgccgaaga acgccgcagg cgtctacgcc
     7981 acgcccgtca tggccgtgca ttcccgaacc atggtgacca ataccgtgcc gattggtccc
     8041 tatcggggcg ccggccggcc ggaagcgaac tacttcatgg aacggttgat ggacgtggcc
     8101 gccgccgaaa ccgggatcga ccgcatcacc ctgcgccggc gcaatcacat ccggccgtcg
     8161 atgctgccgt tcacggcggc gtccggactg gtctatgaca gcggcgactt cccggcggtg
     8221 ctcgaccggg cgctggaagc ctcggattgg aagggctttg gcaagcgcaa ggccgcgagc
     8281 cggcgccggg ggctgctgcg cggcatcggg ctggggcagt tcctggaagg cacggccgc
//
LOCUS       DAOMXN010000102         8331 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_4481_length_8331_cov_8.970759, whole genome shotgun sequence.
ACCESSION   DAOMXN010000102 DAOMXN010000000
VERSION     DAOMXN010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 8331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8331
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4481_length_8331_cov_8.970759"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="PK812_08335"
     CDS             <1..442
                     /locus_tag="PK812_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69601.1"
                     /translation="HPAAGAAVRGLARRGLVTPCPGACPGALTPPQFRRWLRASMRAD
                     METRMRRLTLALVYAGLATSPALAQGGRPSSWTLSCERAQALVAQSGSVVMNFSPTTY
                     DRVVSDLRFCLHGEVLIPAYAPTRDNPQCLVGYTCREGDTRRRR"
     gene            442..768
                     /locus_tag="PK812_08340"
     CDS             442..768
                     /locus_tag="PK812_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69602.1"
                     /translation="MDRATGRFAPARAGLLATGLIGVVATALPAYAQPRPMTSAMTCA
                     DAQALVKRAGARVLNFTPTTYDRVVADLRFCIIGQTLVPEFAPTRDAPRCFVGYVCRE
                     YEIDPG"
     gene            complement(788..1294)
                     /locus_tag="PK812_08345"
     CDS             complement(788..1294)
                     /locus_tag="PK812_08345"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRJ69603.1"
                     /translation="MSGLRIRDATLADVAAVRALLIETWHATYDGTLGAEKVREITDR
                     WHAEAVLAAQVDEGNAAFLVAEREGKLVATASAALGCDRSIDLRRLYVHPSDQGRGVG
                     KELFASCLARFPACQSVRLEVEPRNAAAIAFYRRLGFIEKERGGACGGDEQAAIEHLV
                     MERTLGGP"
     gene            complement(1291..2763)
                     /gene="gatB"
                     /locus_tag="PK812_08350"
     CDS             complement(1291..2763)
                     /gene="gatB"
                     /locus_tag="PK812_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017486316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HRJ69604.1"
                     /translation="MSAHVNPKKLIKGVTGDWEIVIGMEIHAQVTSNAKLFSGASTAF
                     GGEPNSHVSFVDAAMPGMLPVINEECVKQAIRTGLGLKAEINLKSRFDRKNYFYPDLP
                     QGYQISQYKEPIVGEGEVIVDVPGEPEPILVRIERLHLEQDAGKSIHDQDPTNSFVDL
                     NRSGVALMEIVSKPDLRSAEEAQAYVGKLRTILRYLGTCDGDMEKGNLRADVNVSVRK
                     PGADLGTRCEIKNVNSIRFIGQAIEVEARRQIAILEDGGTIDQETRLYDPGKRATRSM
                     RSKEEAHDYRYFPDPDLLPLEFSQGYVDALAAALPELPDAKKARFIAQYGLSPYDASV
                     LIAERESADYFEAVARGRDGKTAANWVINELFGRLNKEGKGIGETPVSATQLGGIIDL
                     IGSGAISGKIAKDLFEIVFTEGGDPAEIVEKRGMKQVTDTGAIEKVVDEIIAANPDKV
                     QQAIAKPTLLGWFVGQVMKASGGKANPGAVNELLKQKLGI"
     gene            complement(2850..3266)
                     /locus_tag="PK812_08355"
     CDS             complement(2850..3266)
                     /locus_tag="PK812_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HRJ69605.1"
                     /translation="MTIMQWIFSLPKEIWLGALLVAGGIYWTMSRIRFVARSATTRGK
                     VVDYDFGFRKKDIVYYPIFEFTAADGSVHRVRSAYHWKRQSLFPNRTYAIRYDPTNPR
                     EAAFRSFKMDFVMPPALVAVGVLLINPRLPGQLLGL"
     gene            complement(3408..3620)
                     /locus_tag="PK812_08360"
     CDS             complement(3408..3620)
                     /locus_tag="PK812_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69606.1"
                     /translation="MPLSRVVLSACLNAFAGLNALAAILLAVLGVLRLVEAGPGGQAV
                     QAAVHAAGFAAGAFVCRWAARKIAAL"
     gene            complement(3633..4340)
                     /locus_tag="PK812_08365"
     CDS             complement(3633..4340)
                     /locus_tag="PK812_08365"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HRJ69607.1"
                     /translation="MRRLVIGLAALCAAGGASAQTAIGEMADIVRQVSGSFGGRTVAL
                     RNTDRVVQNEVLATGADSMARVAFHDLTSVSMGPQATIKLDRFVYNPDSTASQVTLGM
                     AKGAFRFVTGSTKSENFRIRTPQATIGVRGTVVDIWVRPGREIVVLRQGSAWACKGAA
                     CVNIVQPDTGVIITAAGIQGPTPMATAEFNFDVFTLNRFTLYRHPALGNPLGGNGDTQ
                     AGYSSSSSSSSSSTSRQ"
     gene            complement(4422..5597)
                     /locus_tag="PK812_08370"
     CDS             complement(4422..5597)
                     /locus_tag="PK812_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRJ69608.1"
                     /translation="MPTDRPPPSPAARSDVAPFIAMDVMAEAGARQRLGEDVVSLAVG
                     EPGGPPPRAVREAVSAALAAGRVPYTDSLGLPALRERIAKHYAEAYNVAVPASRVAVT
                     TGSSGGFILSFLAMFDVGDRIAIPRPGYPAYRNILQALGLVPVEIPTAAATRYALTGE
                     MIAKAHEKTPLKGALVMSPANPTGVTMAPAALQDVAETCRRLGLWLISDEIYHGLVHA
                     GDTATALAFSDDAVVVNSFSKYYCMTGWRVGWLILPERLVRPVERLAQSLAISVPYLS
                     QIAGQAALGARAELEAIKASYARNRGLLAEALPKLGLPVLPMDGAFYAYVDVSRYSND
                     SMDFSRRALREAGVALTPGVDFDREEGHRAVRISYAGSEADIATAVARLETWLRSHC"
     gene            5808..7172
                     /locus_tag="PK812_08375"
     CDS             5808..7172
                     /locus_tag="PK812_08375"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HRJ69609.1"
                     /translation="MFGRPILAACVVMAALLSPAEAQRQRLNYIRDAEIEQLLKDYLN
                     PILRAAGQPPTAVRLMLVGDRSFNAFVSNGRRMTIYAGAIIDSATPNQLIGVLAHEAG
                     HIAGGHLARLSQELPRAQAIAAIGTLLGAGALVGVATSRNTIGVSGAAPMGILSAGSE
                     LAMRSLLSYQRTEEAAADRAAVNYLTATKQSAKGMLEAFRRLAEQQMFLASRVDKYLL
                     SHPLATERLAAIDAAARESPYFEAKDPPALVQRHELTRAKLIAFLGRQDEIMRRYPPA
                     DASLGARYARAIYAHRFSRGGDAQAQIDALIKAQPNNAYFWELKGQNLLESAQAGAAI
                     APLRKAVTLAPGQPLLRVILGHALMSSGVPANVQQAITELTVAIQRDPEVYEAYVYLA
                     QAYEKAGRTADAELTVAESYFFAGAYEEARLIAKRAQAKFPPGSPGWRRADDIVSFHT
                     DKKG"
     gene            7207..7968
                     /locus_tag="PK812_08380"
     CDS             7207..7968
                     /locus_tag="PK812_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HRJ69610.1"
                     /translation="MLKTIRSALLGAVLAGAALVAPAQALTAQQKTEFETLIRDYILK
                     NPELIQEALIELERRQKEAENAARLKVIKDPNGPLLASKFNFVVGNPKGDVTLVEFFD
                     YNCGFCKRALGDMQRLVKEDANLRIVVKDFPILSAGSREAAAVALAVKQQVKPEKFWE
                     FHVTLMSRSGQIGRAQALEAAKAIGADVARIETDMGKPEVAAAIEEARGLADALGLTG
                     TPSYIVGDEVVVGAVGYEQLKGRIDSLRRCGKTQC"
BASE COUNT         1432 a         2730 c         2796 g         1373 t
ORIGIN      
        1 gcatcccgct gccggcgccg ctgtgcgcgg cctggcgcgg cgagggctgg taactccctg
       61 ccccggggct tgtcccgggg ccttgacgcc gccacagttc cggcgctggc tgcgcgcatc
      121 aatgcgcgcg gacatggaga cccggatgcg gcgactgacg cttgcacttg tttatgccgg
      181 cctggcgaca tcgccggcgc tggcccaagg cggccggcca tcgagctgga cgctgagttg
      241 cgaacgcgcg caggcgttgg tggcgcaatc cggctcggtg gtgatgaatt tctcccccac
      301 cacctatgac cgcgtggttt ccgacctgcg cttctgcctg catggcgagg tgctgatccc
      361 ggcctacgcc ccgacgcggg acaatccgca gtgccttgtg ggctacacct gccgcgaagg
      421 tgacacgcgg aggcggcggt gatggaccgt gcgaccggca ggtttgcgcc ggcgcgtgca
      481 gggctgctgg cgacgggcct gatcggggtt gtggcgaccg ccctgcccgc gtacgcccag
      541 ccgcgcccga tgacatccgc catgacctgc gccgatgcgc aggcactggt caaacgcgcc
      601 ggcgcgcgcg tgctgaattt cacgccgacg acctatgacc gggtggtcgc cgacctgcgt
      661 ttctgcatca tcgggcaaac gctggttccc gagttcgcac cgacgcgcga cgctccgcgc
      721 tgtttcgtgg gctatgtctg ccgggaatac gagatcgatc cgggttgagg cacgaggtga
      781 tcgctgttca tgggccgccg agggtacgct ccatcacgag atgctcgatg gcggcctgtt
      841 catcccctcc gcaggccccg ccgcgctctt tctcgatgaa gccgagacgg cgatagaagg
      901 caatagcagc ggcattgcgc ggttccacct caaggcgaac ggactggcag gccgggaagc
      961 gcgccagaca ggaagcgaac aattcctttc cgacgccccg cccctgatcg gaggggtgga
     1021 cgtaaagccg tcgcaggtca atgctgcgat cacagcctag ggccgctgat gcagtggcga
     1081 caagcttgcc ctcgcgttcc gccaccaaaa aggccgcatt gccttcgtca acctgcgccg
     1141 caagcactgc ctcggcgtgc caacggtcgg tgatctcgcg gaccttttct gctccgagcg
     1201 tgccgtcgta ggttgcgtgc caagtctcga tgagcagcgc gcggaccgcc gcgacatcag
     1261 caagtgtggc gtcgcgaatg cgcagcccgc tcatatcccc agcttctgct tcaaaagctc
     1321 gttcaccgcg ccggggttgg ccttgccgcc cgaggccttc atgacctggc cgacgaacca
     1381 gccgagcagg gtcggcttgg cgatcgcctg ctgcaccttg tccgggttgg cggcgatgat
     1441 ctcgtccacg accttttcga tggctccggt gtcggtgacc tgcttcatgc cgcgcttttc
     1501 gacaatctca gccgggtctc cgccctcggt gaagacgatc tcgaacagat ccttggcgat
     1561 ctttccggag atggcgccag agccgatgag atcgatgatg ccgccgagct gggtggcgga
     1621 aaccggcgtc tcgccgatgc ccttgccttc cttgttgagg cggccaaaca gttcgttgat
     1681 cacccagttg gccgctgtct tgccgtcgcg gcccctggcc acggcctcga agtaatccgc
     1741 gctctcgcgc tcggcgatca gcaccgaggc gtcgtagggc gaaagcccgt attgcgcgat
     1801 gaaacgggcc ttcttggcgt ccggcagttc gggaagcgcg gcggcaagcg catcgacata
     1861 gccctgcgaa aattccagcg gcaacaggtc cggatcgggg aagtagcgat agtcgtgcgc
     1921 ctcttccttg gagcgcatgg agcgggtcgc gcgcttgccc ggatcgtaga ggcgggtttc
     1981 ctgatcgatc gtgccgccgt cctccaggat ggcgatctgc cggcgggcct ccacctcgat
     2041 ggcctggcca atgaagcgga tggaattgac gttcttgatc tcgcagcggg ttccgaggtc
     2101 cgcgccgggc ttgcgcacgg agacgttgac gtcggcgcgc aggttgccct tctccatgtc
     2161 gccgtcgcag gtgccgagat agcgcaggat ggtcctgagc ttgccgacat aggcctgtgc
     2221 ctcttcggcc gagcgcagat ccggcttgga gacgatctcc atcagcgcga caccggagcg
     2281 gttgagatcg acgaaggagt tcgtcgggtc ctgatcgtgg atcgacttgc cggcgtcctg
     2341 ctccagatgc aggcgctcga tgcgcacgag gatcggttcc ggctcgcccg gcacatcgac
     2401 gatcacctcg ccctcgccga cgatcggctc cttgtactgg ctgatctggt agccctgcgg
     2461 caggtcggga tagaagtagt tcttccggtc gaagcgggat ttcaggttga tttccgcctt
     2521 gaggccgagg ccggtgcgga tcgcctgctt cacgcattcc tcgttgatca ccggcagcat
     2581 gcccggcatc gcggcgtcga cgaaggagac gtggctgttc ggctcgccgc cgaaggcggt
     2641 ggaagcgccg gagaacagct tggcgttgga ggtcacctgc gcatggattt ccatgccgat
     2701 gacgatttcc caatcgccgg tgacgccctt gatcagcttc ttcggattga catgcgcgga
     2761 catgatggaa agcctcggct cggccccgga cgcggggcga tcatgccgga gggttcaccg
     2821 caaaccgcga tccgggtcaa gcccgatgat cacaggccca gcagctggcc ggggaggcgc
     2881 gggttgatca acagcacgcc aaccgcgacc agggccggcg gcatgacgaa atccatcttg
     2941 aagctgcgga aggcggcctc gcgcgggttc gtcggatcgt agcggatggc gtaggtgcgg
     3001 ttcgggaaca ggctctggcg cttccaatga taggccgagc ggacccggtg cacgctgccg
     3061 tcggcggccg tgaactcgaa gatcggatag tagacaatgt ctttcttgcg gaagccgaag
     3121 tcataatcca ccaccttgcc acgcgtcgtc gccgagcggg ccacgaaccg gatgcgcgac
     3181 atcgtccagt aaatgccacc agcgaccagc aaagcgccaa gccagatctc cttcggcagg
     3241 ctgaaaatcc attgcatgat ggtcatgatg ctccccggtg gccgcaggtg tcatgctgtc
     3301 acgcagcacc atggcagcgg gttaaccggc gcgccaaaag cgccactatc gttatccaga
     3361 gcttgccgcg ctgtcggaca ccgggtcacc cgacgtgacc gataggatca gagggcggcg
     3421 atcttcctgg ccgcccagcg gcacacgaag gccccggcag cgaacccggc agcgtgcacc
     3481 gcagcctgga ctgcctgccc gcccggtccg gcctctacca gtcgcagcac gcccagcacg
     3541 gcaagcagga tggcggcgag cgcattcagt ccggcgaagg cgttcaggca ggccgagagg
     3601 acgacacggg acagcggcac cggattgaat cgctactggc ggcttgtcga ggaggaggac
     3661 gacgacgagg aactcgaata cccggcctgg gtgtcgccat tgccgccaag cgggttcccc
     3721 agcgccggat gccggtaaag ggtgaagcgg ttcagggtga agacatcgaa attgaactcg
     3781 gcggtggcca tcggcgtcgg cccctgaatg ccggcggcgg tgatgatcac gccggtatcg
     3841 ggctgcacga tgttgacgca ggccgcgccc ttgcaggccc aggccgatcc ttgccggagc
     3901 accacgatct cgcggccggg ccggacccag atgtcgacca cggtgccacg gacgccgatc
     3961 gtcgcctgcg gcgttcggat gcggaagttc tcggatttcg tgctgcccgt gacgaatcgg
     4021 aatgcgccct tcgccatgcc cagcgtgacc tggctggcgg tgctgtccgg attgtagacg
     4081 aaacggtcga gcttgatcgt ggcctgaggc cccatcgaca cgctggtgag atcgtggaag
     4141 gcgacgcgcg ccatgctgtc cgcgccggtc gcgagcacct cgttctggac aaccctgtcg
     4201 gtgttcctga gcgcgaccgt cctgccgccg aagcttcccg agacctgccg gacaatatcg
     4261 gccatctcgc cgatcgcggt ttgcgccgaa gcgccgccag ccgcgcagag ggcggccagc
     4321 ccaatcacca atcgccgcat gttcgaaacc tttccgtccg gttctcaaag gccccggcgg
     4381 caaagatcgg tcaaaaccgt aaaaaagtca ctggtattgc ttcagcaatg cgagcgaagc
     4441 caggtctcca gccgcgccac tgccgtggcg atgtccgctt cgctgccggc atagctgatg
     4501 cggacggcgc gatggccttc ctcgcggtcg aagtcgacgc ccggcgtcag cgcgacaccg
     4561 gcctcgcgca gggcacggcg actgaaatcc atcgaatcgt tgctgtagcg ggaaacatcg
     4621 acataggcgt agaaggcccc gtccatcggc aggacgggca ggccgagctt tggcagcgcc
     4681 tcggccagca ggccccggtt gcgggcatag ctggccttga tcgcctcaag ttccgcgcgc
     4741 gcgccgagtg cggcctgccc cgcgatctgg ctgaggtaag gaacggaaat ggcgagactc
     4801 tgcgccaggc gctccaccgg ccgcaccagc cgttccggca ggatgagcca gccgacacgc
     4861 cagccggtca tgcagtagta cttggagaac gagttcacga cgaccgcgtc atcggaaaag
     4921 gccagagcgg tggcagtgtc cccggcatgc accagaccgt ggtagatttc atccgagatc
     4981 agccagagcc ccaggcggcg acaggtctcg gccacatcct gcagggcggc gggcgccatg
     5041 gtgacgccgg tcggattggc gggcgacatg accagcgcgc ccttgagcgg ggtcttctcg
     5101 tgcgcctttg cgatcatctc gccggtcaga gcgtagcggg tcgccgccgc ggtcgggatt
     5161 tccacgggaa cgagacccag cgcctgcagg atgttgcggt aggcgggata gcccggccgc
     5221 gggatggcaa tgcggtcgcc cacatcgaac atcgcgagga acgacaggat gaagccgccg
     5281 gaagagccgg tcgtgaccgc gacgcggctt gccggaaccg ccacattgta ggcctcggca
     5341 tagtgcttgg cgatgcgctc acgcagcgcc ggcaggccca gcgagtccgt atacggcacc
     5401 cggcctgcgg caagtgcggc gctcacggcc tcgcgcaccg cacgcggcgg cggcccgccg
     5461 ggttcgccca cggccagcga aacgacatcc tcacccaggc gctggcgcgc accggcttcc
     5521 gccatgacgt ccatggcgat gaagggggcg acgtcggatc gggcggcggg agaaggtggc
     5581 ggtctatcgg tcggcatcgg gcagtgtctc cggcctcctg cataggccgc ggcgggccga
     5641 tgccgcaaga aggccttgtt gccgtgttcc cgatgggggg cgcagcggtc acaacccggc
     5701 gatcccgccg gcgtcgcgtt gaccgctcgc gggcagcttc ctaggctccg gccgatgttt
     5761 cgaggtgtct tgtgaatcga ttcgcgcccc tgcggcgcct cttatccgtg ttcggccgcc
     5821 cgatcctggc ggcctgcgtc gtcatggccg cgctgctgtc gccggcggag gcgcagcgac
     5881 agcggctcaa ctacatccgc gatgccgaga tcgagcagtt gctgaaggac tacctcaacc
     5941 cgatcctgcg ggcagccggc cagccgccga cggccgtccg gctcatgctg gttggcgatc
     6001 gctccttcaa cgccttcgtg tccaatggcc ggcgcatgac gatctacgcc ggtgcgatca
     6061 tcgattccgc cacgccgaac cagctcatcg gcgttctggc gcatgaagcc gggcacatcg
     6121 cgggcggcca tctggcgcgg ctcagccagg aactgccgag ggcgcaggcg atcgccgcca
     6181 tcggcacatt gctcggtgcc ggcgctctcg tcggcgtggc gacctcgcgc aacaccatcg
     6241 gcgtgagcgg cgcagcgccg atgggcatcc tgtcagcggg ttccgaactg gcgatgcgca
     6301 gcctgctctc ctaccagcgg acggaggagg cggcggcgga ccgcgcggcg gtgaactacc
     6361 tgacggcgac gaagcaatcg gccaagggca tgctggaagc gttccggcgg ctcgccgagc
     6421 agcagatgtt cctcgcctcg cgggtggaca agtacctgct cagccatccg ctggcgacgg
     6481 agcggctggc ggcgatcgac gcggcggcgc gggaaagccc ctacttcgag gcaaaggacc
     6541 cgcccgcgct cgtccagcgc catgaactga cgcgggccaa gctgattgcc ttcctcgggc
     6601 ggcaggacga gatcatgcgg cgctatccgc cggcggacgc ctcgctcggc gcacgctacg
     6661 cccgggcgat ctatgcgcac cggttttcgc gcggcggcga cgcgcaggcc cagatcgacg
     6721 cgctgatcaa ggcgcagccg aacaacgcct acttctggga gctgaaggga cagaacctgc
     6781 tggaaagcgc gcaagcgggg gccgccatcg cgcccctgcg caaggcggtc acgctggccc
     6841 cgggtcaacc gctgctgcgt gtcatcctcg gccacgcgct gatgtcctcc ggcgtgccgg
     6901 ccaacgttca gcaggccatc acggaactga cggtggcgat ccagcgcgat ccggaagtgt
     6961 atgaagccta tgtttacctg gctcaggcat acgaaaaggc aggccgcacg gccgatgccg
     7021 aactcacggt ggcggagagt tacttttttg caggcgcgta tgaagaagca cgcctgatcg
     7081 caaagcgggc gcaggccaag ttccctccgg gttcacccgg ctggagacga gccgacgaca
     7141 tcgtttcgtt ccacacggac aagaagggct gatcccggtt ccggcatcgg cgcaggagac
     7201 aagacaatgc tgaagaccat ccgttccgcg ctgctcggag ccgtgcttgc cggcgccgcc
     7261 ctggttgcgc ccgcccaggc gctcacggcg cagcagaaga ccgagttcga gaccctgatc
     7321 cgcgattaca tcctgaagaa tccggaactg atccaggaag cgctgatcga actggaacgg
     7381 cgccagaagg aggcggaaaa cgccgcccgc ctcaaggtca tcaaggaccc gaacgggccg
     7441 ctgctcgcct cgaagttcaa ttttgtcgtc ggcaacccga agggcgacgt cacgctcgtc
     7501 gagttcttcg actacaactg cggcttctgc aagcgcgcgc tcggcgacat gcagcgcctg
     7561 gtgaaggagg acgcgaacct gcgcatcgtg gtgaaggatt tcccgatcct ctccgccgga
     7621 tcccgcgagg ccgccgccgt cgcgctggcg gtgaagcagc aggtcaagcc cgagaagttc
     7681 tgggagttcc acgtcaccct gatgagccgg tccggacaga tcggcagggc gcaggcgctg
     7741 gaggcggcca aggccatcgg cgccgatgtg gcgcgcatcg agaccgacat gggcaagccg
     7801 gaagtcgcgg ccgcgatcga ggaagcgcgc ggcctggccg atgcgctggg cctgacaggc
     7861 acgccgtcct acatcgtcgg tgatgaggtc gtggtcggtg ccgttggcta cgagcagctc
     7921 aaggggcgca tcgattcctt gcgccgatgc ggcaagaccc agtgttgagc gcctgcggca
     7981 accggacttc atcagcacca gacattccca aaggccggcg gtgacgccgg ccttctgtcg
     8041 cgcggagtgc tgaagaccgc gtgtcggcgg tctgccggca agtcactgcc cccctcccgc
     8101 aatgcacaag cgagcctttt catccggcgc gcgccgggct atgacgcgcg gccgatcttg
     8161 aacgcgccgg gcacaaggcc cgggcggaac catgagagac gaatgtaatg gctggcaaag
     8221 gccccttcga caccgatatc gttcgcgaac tcgccaaact gattaccgaa accgatctcg
     8281 acaagctcgc caacggcggt gcgaaactgg agcagtttta cgtgctgccg g
//
LOCUS       DAOMXN010000103         8295 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_4501_length_8295_cov_10.121966, whole genome shotgun sequence.
ACCESSION   DAOMXN010000103 DAOMXN010000000
VERSION     DAOMXN010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 8295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8295
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4501_length_8295_cov_10.121966"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="PK812_08385"
     CDS             complement(<1..637)
                     /locus_tag="PK812_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69611.1"
                     /translation="MMPYPAVMDVASLAVLATIALFAAPGPTNALLFAAGAGRGFARS
                     LVLAGVVLAAYTLAVGSARLALEPLIASHDVAARALRLVLGLYVLWLALRLWRSTLNA
                     AIPVAIDAGSLFLTTLLNPKALVLAFLLLPPAGAALASALAVAVGAILVSSLLWIGLG
                     HAEVVADDQVGLGVGVLDGRRDRAAGGRAEGVDHDDEADGDRHGQQGGHVPP"
     gene            725..1852
                     /locus_tag="PK812_08390"
     CDS             725..1852
                     /locus_tag="PK812_08390"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HRJ69612.1"
                     /translation="MSALRSSLSRARDFLDRIALVDAHNDLPYVIRAESAAKGDVDAY
                     RLDRVHQETDTDIPRLRKGKVAGQFWAAFVPTSVSHPATFTLEQIALVKRMNARHADV
                     FLPALKASDVARARRTGRIASFICVESGVGLENSLEVLALWHELGVRYLTLCHNETLD
                     WVDSSTDAPRHGGLTEFGKAVIRECNRLGILVDLAHTSHEAQLTALDVATAPVAITHA
                     NAFSLVDHPRNVRDAVLARIRATGGIVCATFVPTFLNQAVRDWVKPLCDPYGKPLGGS
                     TTDAIQRHEAMAGPCPRASVEDVCDHIDYMTARLGIDHIGIGSDFYGGRNPEGLEDVS
                     TFPALIAALMDRGWGEPALKKLVSGNLLRVLRAVEKAAAPR"
     gene            complement(1870..2073)
                     /locus_tag="PK812_08395"
     CDS             complement(1870..2073)
                     /locus_tag="PK812_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020180897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YARHG domain-containing protein"
                     /protein_id="HRJ69613.1"
                     /translation="MSCDELWFYRNSIYKNAGYCFKTTRAIRAFGNAGCQFDEMELVP
                     LSSADRREVSAVQQAERRKRCPR"
     gene            complement(2246..3307)
                     /locus_tag="PK812_08400"
     CDS             complement(2246..3307)
                     /locus_tag="PK812_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HRJ69614.1"
                     /translation="MIGRLSIRIVVLGLLAVGLTACAQYHPEQPKGTAIVRLGGYDST
                     VQRDMARDPLKASELAGYYVPYALMAVRAYGDADAAGERWQALIEDKDDRGRPLAGQV
                     AAWLADWHHAGSVDNPRAQHHENGPLTCGRDRKLIGQCLFNLPGMAYEIWVRKQCREV
                     VLAFRGTDFREINDWLTNFHWFLRLLPINDQYEQVQDHTRRLVDQMRRLCGGKSPLIT
                     AVGHSLGGGLAQQAAYVDNRITRVIAFDPSIVTGYHDVHSDTRDLSRLKIDRVYEHGE
                     VLAYLRFVQRQFAPASACNPQIRTIRVNLDRGSVVSQHSMATFAANLVRAAGTPRDWQ
                     ARRKYVLPESAPDLCHRPA"
     gene            complement(3375..5531)
                     /locus_tag="PK812_08405"
     CDS             complement(3375..5531)
                     /locus_tag="PK812_08405"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HRJ69615.1"
                     /translation="MKTVSTHGLTVAQNLFDFVNKEALPGTGVTQEQFWSGLAGVLKD
                     FAPKNRRLLKVRDRLQKQIDAWHAARKGKAFKQGEYEAFLKEIGYLRPEPGEGVVKTK
                     NVDAEIARIAGPQLVVPVSNARYALNAANARWGSLYDALYGTDAIPDEGAAARGKGYN
                     PARGALVVAKAKAVLDQAAPLTKGSHADATGYRVENGALVVEMGKKTAKLANRKAFAG
                     YTGDAGKPGSILIAHNGLHFIIKIDRAHAIGKTDPAGVADVIAESALTTIMDMEDSVA
                     AVDAEDKVEVYRNWLGLMKGTLTADMEKDGKKLVRKLNGDLSFTAPKGGTVTLPGRSL
                     MLVRNVGKHMMIDMVRDEKGKDMPETAIDCAVASLIALHDLKKKDGIRNSRAGSVYIV
                     KPKMHGPDEVKLADRLFGKVEAMLGMAKNTLKMGIMDEERRTTINLSACILAAKNRVV
                     FINTGFLDRTGDEIHTSMEAGPMVRKGDIRTSVWLSAYEDSNVDVGMLRGLPGKAQIG
                     KGMWAMPDRMADMLKAKVGHPKAGANTAWVPSPTAATLHALHYHEVDVFARQKELASR
                     ARAKLSDILTIPLARSNFDPEAVKQELDNNAQGILGYVVRWVDQGVGCSKVPDIHDVG
                     LMEDRATLRISSQHIANWLHHGVCTRAQVRSTLKRMAKVVDRQNAGDPAYKPMAPGYD
                     GVAFKAACDLVFKGLKQPNGYTEFLLTRARRAAKAG"
     gene            5701..5943
                     /locus_tag="PK812_08410"
     CDS             5701..5943
                     /locus_tag="PK812_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69616.1"
                     /translation="MGQILVRNLDDGVIARLKSKAKRDGKSLEQTAREALTSAVRPSR
                     AEVIAELDRIRAMSKPSAVTSVELLRAFRDGDDPDC"
     gene            5927..6325
                     /locus_tag="PK812_08415"
     CDS             5927..6325
                     /locus_tag="PK812_08415"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRJ69617.1"
                     /translation="MTLIVDASVAAKWLFDEIGSAEAHAIAERPDLVAPEFLMLECHH
                     ILWKRYRRQETTWAAVIELQVGLQLAFETFFPDADLRPRAVELSLSHSLAIYDCLYIA
                     LARSQRAPLITADDRQFAAARKAGVEVEML"
     gene            complement(6570..8102)
                     /locus_tag="PK812_08420"
     CDS             complement(6570..8102)
                     /locus_tag="PK812_08420"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HRJ69618.1"
                     /translation="MKDILEKLEVRRANARIGGGEARIKAQHAKGKLTARERIELLMD
                     AGSFEEFDMFVEHRSTEFGMEKTKMPGDGVVTGWGTVNGRTVFVFAKDFTVFGGSLSE
                     THAQKICKIQDMALKMRAPIIGLFDAGGARIQEGVAALGGYGEVFKRNVIASGVIPQI
                     SLIMGPCAGGDVYSPAMTDFIFMVRDTSYMFVTGPEVVKTVTNETVTSEELGGASIHT
                     TKSSVADGAYDSDLEALLQMRRLIDFLPANNTDGAPQWPSFDMIDRVEMSLDTLVPDN
                     PNKPYDMKELILKVVDEGDFFEIQESFAKNIITGFARIEGRSVGFVANQPMVLAGVLD
                     ADASRKAARFVRFCDAFNIPIVTFEDVPGFLPGTAQEYGGLIKHGAKLLFAYAQCTVP
                     LVTIIVRKAYGGAYDVMASKHIGGDVNYAWPTAQIAVMGAKGAVEIIFRQDIGNAEKI
                     AQRTKEYEERFLSPFVAAERGYIDEVIMPHGTRRRIARALAMLRTKKLEQPWKKHDNL
                     PL"
BASE COUNT         1409 a         2799 c         2615 g         1472 t
ORIGIN      
        1 ccggcggtac gtggccgccc tgctggccgt gacgatcgcc gtcggcttcg tcgtcgtgat
       61 cgacgccctc ggctcgtccg cccgcggcgc gatctcggcg gccgtcgagg acgcctaccc
      121 cgaggccgac ctgatcgtcg gcgacgactt cggcgtggcc gagcccgatc cagagcaggc
      181 tcgacacaag gatcgcaccc accgcaaccg caagcgccga cgccagcgcc gcgcccgccg
      241 gcggcagcag caggaaggcc agaaccagcg ccttgggatt gagcagcgtc gtcaggaaca
      301 gcgatcccgc atcgatcgcc accggtatcg ccgcgttcag ggtgctgcgc cacagccgga
      361 gcgccagcca cagcacatag agtcccagca ccagccgtaa cgcacgggcc gccacatcgt
      421 ggctggcgat caagggttcg agcgccaggc gggcgctgcc gacagccagc gtataggcgg
      481 ccagcaccac tccggcgagc acgagggaac gcgcaaaccc ccgcccggcc ccggcggcga
      541 acagcagggc attcgttggg cccggcgcag cgaacagcgc gatggtggcg agcacggcaa
      601 gggaagcgac gtccatcacg gccggatagg gcatcaagcc gaaacctcca ttgattccgc
      661 gcaatcgctt ccggcagtcg tcgtcatctg cgatcctgct tcaggaatca ctggggaacc
      721 cgccatgtcc gccctgaggt ccagcctctc ccgcgcccgc gactttctcg atcgcatcgc
      781 ccttgtcgat gcgcacaacg acctgcccta tgtcattcgc gcggaatcgg cggcgaaggg
      841 agacgtcgat gcctaccggc tggaccgggt gcaccaggaa accgacaccg acattccgcg
      901 cctgcgcaag ggcaaggtcg ccggccagtt ctgggcagcc ttcgtgccga cgagcgtcag
      961 ccatcccgcg actttcacgc tggaacagat cgcgctggtc aagcgcatga acgcccgcca
     1021 cgccgacgtg ttcctgcctg ccttgaaggc atccgacgtc gcgcgtgccc ggcgcacggg
     1081 caggattgcc tcgttcatct gcgtcgaaag cggcgtcggg ctggagaatt cgctcgaagt
     1141 gctcgcgctc tggcacgaac tcggcgtgcg gtatctgacg ctctgccaca acgaaacgct
     1201 ggactgggtg gattcgtcca ccgacgcgcc acggcacggc gggctgaccg aattcggcaa
     1261 ggcggtgatc cgcgagtgca accgcctcgg catcctcgtc gacctcgccc acacctcgca
     1321 tgaagcgcag ctgaccgcgc tggatgtcgc gaccgcgccg gtggcgatca cccacgccaa
     1381 tgccttctcg ctggtggatc atccgcgcaa tgtccgcgac gcagtgctgg cccgcatccg
     1441 cgccacgggt ggcatcgttt gcgccacttt cgtgccgacc tttctgaacc aggccgtccg
     1501 cgactgggtc aagccgctgt gtgaccccta cggcaagccg ctcggcggct ccaccaccga
     1561 cgccatccag cgtcacgagg ccatggcagg cccctgcccg cgcgccagcg tcgaggatgt
     1621 ctgtgatcac atcgactaca tgaccgcccg cctcggcatc gaccacatcg gcatcggctc
     1681 ggatttctat ggcggtcgca acccggaagg gctggaagac gtctcgacat tcccagcgct
     1741 catcgccgcg ctgatggatc gcggctgggg cgaaccggcc ctgaagaagc tggtttccgg
     1801 caatctgctg cgcgtgctgc gcgcagtgga aaaagcggcc gcaccgcgtt agcagccagc
     1861 ctccgattgc tacctggggc agcgcttgcg ccgttccgcc tgctgaaccg cggagacctc
     1921 acggcgatcc gcagacgaga gcggcaccag ttccatctcg tcgaactggc agccggcatt
     1981 gccgaaggcg cggatcgccc gcgtcgtctt gaagcagtag ccggcgttct tgtagatcga
     2041 gttgcgatag aaccagagtt cgtcgcagct catggcgtca tggttcagtt gcgccgaggc
     2101 gcccggaacc agcgcggcgg aaaaggcggc ggcaagaatc cagcgctttg gcatggcggc
     2161 atcccgtgca ggtcgacgcc ggcaggaaag ccccgccacc gcgacagtgc aatgaggaag
     2221 cgcaagcctg ggggagacac cgcgcctagg ccggccgatg acaaaggtcc ggagcgctct
     2281 cgggcagcac gtacttccgg cgtgcctgcc aatcccgcgg tgtcccggcg gcgcggacca
     2341 gattggccgc gaacgtcgcc atcgaatgct gcgacaccac cgatccccga tccaggttga
     2401 cacggatggt gcggatctgc ggattgcaag cgctggccgg cgcgaactgg cgctggacga
     2461 accgcaggta ggccagcact tcgccatgct cgtagacacg atcgatcttc agccggctga
     2521 gatcgcgcgt atccgaatgc acgtcatggt atcccgtcac gatgctcgga tcgaaggcga
     2581 taacgcgggt gatgcggttg tcgacatagg cggcctgctg ggccagaccg ccccccagag
     2641 agtgtccgac ggcggtgatg agcggggact ttcccccgca cagccggcgc atctggtcca
     2701 ccagacggcg cgtatgatcc tgcacctgtt cgtactggtc attgatcggc aacaggcgaa
     2761 ggaaccagtg gaaattcgtc agccagtcgt tgatctcacg aaaatccgtg ccgcgaaacg
     2821 caagcacgac ctcgcggcac tgtttgcgca cccagatttc ataggccatg cccggcaggt
     2881 tgaacaggca ctgaccgatc agcttgcggt cccggccgca ggtcagcgga ccattctcgt
     2941 ggtgctgtgc acggggattg tcgacgctgc ccgcatggtg ccagtctgca agccaggccg
     3001 cgacctggcc ggccagtggg cgccccctgt cgtccttgtc ctcgatcaaa gcctgccagc
     3061 gctcgccggc ggcatcggcg tcgccatagg cgcgcaccgc catcagcgcg tacggcacgt
     3121 agtagcctgc cagctcgctg gccttcagcg gatcccgcgc catgtcccgc tggacagtgc
     3181 tgtcgtaacc gcccagtcgc acgatcgccg tgcctttggg ctgttccggg tgatactgcg
     3241 cgcacgcggt caacccgacc gcaagaaggc ccagcaccac gatccgtatc gacaagcgtc
     3301 cgatcatggc ccgcgccccc cgttcccatc aaagatcagg gcgtggcgga tttcaagccg
     3361 gcatgcctgg cggttcaccc cgccttcgcc gcccgccgcg ccctggtgag caggaactcg
     3421 gtgtatccgt tcggctgctt cagccccttg aagaccagat cgcaggccgc cttgaacgcg
     3481 acgccatcat agcccggcgc catcggcttg taggccgggt cgccggcgtt ctgccggtcc
     3541 acgaccttgg ccatgcgctt cagcgtcgag cgcacctgcg ccctggtgca cacgccgtga
     3601 tgcagccagt tggcaatgtg ctgggaggag atacgcagcg tcgcgcggtc ctccatcagg
     3661 ccaacatcgt ggatgtccgg caccttggag cagccgacgc cttggtcgac ccagcgcacg
     3721 acatagccaa gaatgccctg cgcgttgttg tcgagctcct gcttgaccgc ctccggatcg
     3781 aaattcgatc tcgccagcgg gatggtgagg atgtccgaaa gcttggcccg cgcccggctc
     3841 gcgagctcct tctggcgggc gaacacatcc acctcgtggt agtgcaacgc atgcagcgtc
     3901 gcggccgttg gcgagggcac ccaggcggtg ttggcgccgg ccttcggatg gccgaccttg
     3961 gccttcagca tgtcggccat gcgatccggc atcgcccaca tgcccttgcc gatctgcgcc
     4021 ttgcccggca ggccgcgcag catgccgaca tcgacgttcg aatcctcata ggcggagagc
     4081 cagaccgagg tgcggatgtc gcccttgcgc accatcggcc cggcttccat cgaggtgtgg
     4141 atctcgtcgc cggtgcgatc aaggaagccg gtgttgatga acaccacgcg gttcttcgcg
     4201 gcgagaatgc aggccgagag gttgatcgtc gtgcgacgct cctcgtccat gatgcccatt
     4261 ttcagcgtgt tcttcgccat gccgagcatg gcctcgacct tgccgaacag ccggtccgcc
     4321 agcttcacct cgtccgggcc gtgcatcttc ggcttgacga tatagaccga gccggcgcgg
     4381 ctgttgcgaa tgccgtcctt cttcttcagg tcgtgcagag cgatcagcga agcgacagcg
     4441 caatcgatcg ccgtctcggg catgtccttg cccttctcgt cgcgcaccat gtcgatcatc
     4501 atgtgcttgc cgacgttgcg caccagcatc agcgagcggc ccggcagcgt cacggtcccg
     4561 cccttcggcg cggtgaagct gagatcgccg ttgagcttgc ggacaagctt cttgccgtcc
     4621 ttctccatgt cggcggtcag cgtgcccttc atcaggccga gccagttgcg gtagacctcg
     4681 accttgtcct ccgcatccac cgccgcgacc gaatcctcca tgtccatgat ggtggtcagc
     4741 gcggattcgg cgatcacatc ggcgacgccg gccgggtcgg tcttgccgat ggcgtgggcg
     4801 cggtcgatct tgatgatgaa gtgcagcccg ttgtgggcga tcaggatcga gcccggcttg
     4861 ccggcatcgc cggtgtagcc ggcgaaggcc ttcctgttgg ccagcttcgc cgtcttcttg
     4921 cccatctcga cgacaagcgc gccgttctcc acgcgatagc ccgtggcgtc ggcatgcgag
     4981 cccttggtca gcggcgcggc ctgatccagc acggccttgg ccttggcgac gacgagcgcc
     5041 cctcgcgcgg ggttgtagcc cttgccccgc gccgcagcgc cctcgtccgg aatggcatcc
     5101 gtaccgtaga gcgcgtcata gaggctgccc cagcgggcgt tggcggcgtt cagcgcgtag
     5161 cgggcattcg agaccggcac cacgagctgc ggtccggcga tgcgggcgat ctcggcgtca
     5221 acgttcttcg tcttgaccac gccctcgccg ggctccggcc ggagatagcc gatctccttc
     5281 aggaaggcct cgtattcgcc ctgcttgaag gccttgccct tgcgcgcggc atgccaggcg
     5341 tcgatctgct tttgcagcct gtcgcgaact ttcagcagcc gccggttctt cggcgcgaaa
     5401 tccttcagaa ccccggcgag gcccgaccag aactgctcct gtgtcacgcc cgtgcccggc
     5461 agcgcctcct tgttgacgaa gtcgaagagg ttctgcgcga cggtcaggcc gtgggtcgag
     5521 acggttttca tggggcgtcc ttcggttgaa tcgtggccgc ctctaaatca cagcggtcag
     5581 gcggcgaaaa gggcgaagcg accgaaggat ttccaaaatc gcaaggcgaa tggggcgatt
     5641 tcgttgaaat ccgtcaaccg catgctatga tagcatcctg ctatcatccg gaggtccgcc
     5701 atgggccaga ttcttgtgcg caatctcgac gacggcgtca tcgcccggct caagtccaag
     5761 gccaagcgcg acggcaaatc gctggagcag acggcgcgcg aggccctcac ctccgccgtc
     5821 cgtccttcgc gcgcggaagt gatcgccgaa ttggaccgca tccgggcgat gagcaagccc
     5881 tcggccgtca ccagcgttga acttctccgc gccttccggg acggcgatga ccctgattgt
     5941 tgacgcctct gttgccgcca aatggctgtt tgacgagatt ggcagcgccg aggcgcacgc
     6001 cattgccgag cggcccgact tggtcgcgcc ggagttcctg atgctggaat gccaccatat
     6061 cctgtggaag cgctacaggc gacaggaaac gacctgggcc gccgtcatcg aactgcaagt
     6121 ggggctgcaa ttggcgttcg agacgttttt tccggatgca gacttgcgcc cgcgcgcggt
     6181 cgaactcagc ctctcccaca gcctcgccat ctacgattgc ctttacatcg ccctcgcccg
     6241 gagccagcgc gcgccgctca tcaccgccga cgaccgccag ttcgccgccg cgcgcaaggc
     6301 cggggtcgag gtagagatgt tgtgagggcg ggggtcggcg cgacaatgca ggcccagcgg
     6361 cgctgtcatt ccggggcgct ccttcaggag cgagcccgga atccatcttt cttcaggcca
     6421 ggcgcaaaat atggattccg gacttggctg cgccaatccg gaatgacacc gcgacgcggc
     6481 aatccagaaa aagcgctgca gaagagggct ggattgcttc gttgggcata gccctcctcg
     6541 caatgacggg cgccccctgc ctcctgccct cacagcggca ggttgtcgtg cttcttccaa
     6601 ggctgttcca gcttcttggt gcgcagcatg gccagcgccc gggcgatgcg ccggcgggtg
     6661 ccgtgcggca tgatcacctc gtcgatgtag ccgcgctcgg ccgcgacgaa gggcgagagg
     6721 aagcgctcct cgtattcctt tgtgcgctgg gcgatcttct cggcgttgcc gatgtcctgc
     6781 cggaagatga tttccaccgc gcccttcgcg cccatcaccg cgatctgcgc cgtcggccag
     6841 gcgtagttga cgtcgccgcc gatgtgcttt gaggccatca cgtcataggc gccgccatag
     6901 gccttacgca cgatgatcgt caccaacggc acggtgcact gggcataggc gaacagcagc
     6961 ttcgcgccgt gcttgatcag gccgccgtat tcctgcgccg ttcccggcag gaagcccggc
     7021 acgtcctcga aggtgacgat ggggatgttg aaggcgtcgc agaagcgcac gaaccgcgcc
     7081 gccttgcggc tggcgtcggc gtccaggacc ccggccagca ccatcggctg gttggcgacg
     7141 aagccgaccg agcgcccctc gatgcgcgcg aagccggtga tgatgttttt cgcaaaactc
     7201 tcctggattt cgaagaaatc gccctcgtcc acaaccttca ggatcagttc cttcatgtca
     7261 tagggcttgt tcggattgtc cggcaccagc gtgtcgaggc tcatctcgac gcggtcgatc
     7321 atgtcgaagg agggccattg cggcgcgccg tcggtgttgt tggccggcag gaagtcgatc
     7381 agccggcgca tctgcagcag cgcctcaagg tcgctgtcat aggccccgtc cgccacggaa
     7441 gacttggtgg tgtggatcga ggcgccgccg agctcctccg aggtcacggt ctcgttggtg
     7501 acggtcttca ccacctccgg cccggtgacg aacatgtagg aggtgtcgcg caccatgaag
     7561 atgaaatcgg tcatcgccgg ggaatagacg tcgccgccgg cgcaggggcc catgatcagc
     7621 gagatctgcg ggatcacgcc cgaggcaatg acgttgcgct tgaacacctc gccatagccg
     7681 ccgagcgcgg cgacgccttc ctgaatgcgt gcgccgccgg catcgaacag gccgatgatc
     7741 ggggcgcgca tcttcagcgc catgtcctga atcttgcaga tcttctgcgc atgcgtctcg
     7801 gagagcgagc cgccgaacac ggtgaaatcc ttggcgaaga cgaagaccgt gcgcccgttc
     7861 accgtgcccc agccggtgac gacgccgtcg cccggcatct ttgtcttttc catgccgaac
     7921 tcggtggagc gatgctccac aaacatgtcg aattcctcga aggagccggc atccatcaaa
     7981 agctcgatgc gctcccgggc ggtcagcttg cctttggcgt gctgtgcctt gatgcgcgcc
     8041 tcgccgccgc cgatgcgggc gttggcgcgg cggacttcca gcttttcgag aatgtccttc
     8101 atggtctcac cccttatgtc gaaagtcgcg acaagcgacc gttcggcgcc actcaaaaat
     8161 ttctcagcca gcggatcaag atgagaaacc ttttgaaagg aaccaaatct cgacgggttc
     8221 cccgtcctga gatttggcgt cacatcgttg gcaagcggca taacatcggg gcggccacgg
     8281 tcaatccgcc ctttt
//
LOCUS       DAOMXN010000104         8290 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_4509_length_8290_cov_11.136369, whole genome shotgun sequence.
ACCESSION   DAOMXN010000104 DAOMXN010000000
VERSION     DAOMXN010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 8290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8290
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4509_length_8290_cov_11.136369"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /locus_tag="PK812_08425"
     CDS             <1..932
                     /locus_tag="PK812_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRJ69619.1"
                     /translation="GEKWILGPCRSSAHSWPILSGTMDFGMGHWTSFAQVLVSRLGVP
                     IHAIRLAQGDSDRLNVGGGSGGSKSMQVAGEAIVQASAVVIEKGRILASHLFEAGVDD
                     IAFANGVFRVAGTDRGIAILDLAARLRAGTLRLPEDAPKTLDADAVIDGVPMTFPNGC
                     HVAEVEIDPETGATRVARYTAVTDSGTVVNPMLLEGQVHGGVVQGIGQALMEHTVFDE
                     HGQLLTGSFMDYCLPRADTVPFIASESLPSPARSNTLGIKGVGESGCAGALTSVMNAV
                     VDALSVYGIRHLDMPATPERVFAAIRAARDGRS"
     gene            1053..1358
                     /locus_tag="PK812_08430"
     CDS             1053..1358
                     /locus_tag="PK812_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69620.1"
                     /translation="MGDMKDSLGASGADLGVRTIIAQLEAASTLLKFAADDLESYADR
                     ADTGVRDSLRGIVRALQKNAASLFENALYLDRVEQRDDEDEIAGWPLPSPARRASQA"
     gene            1488..2783
                     /locus_tag="PK812_08435"
     CDS             1488..2783
                     /locus_tag="PK812_08435"
                     /inference="COORDINATES: protein motif:HMM:NF012874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HRJ69621.1"
                     /translation="MSSVSFSKAVRQNLISLQETAEKISVAQNRLSTGKKVNSALDNA
                     TNFFTAAGLNSRANDLSLLLDGISNAVKTVDAANNGITSITRLVESAQSTARQALSDK
                     SSASYNSLEGAATINLTGSTGTTAKDQGMNQTLANVGFADGDTFSLSTTKNGVTKSFS
                     YDLTTVGTKKVSDLVAAINASGVATAEVTDAGKLKISGAADEQVALQLNSADGNTTAG
                     NADVATGLGFTQSTGGTTTVTNAEGAANGDRDSTLITLAAGTASVNRAALVTQYNEIL
                     KQVNKLADDASFNGVNLLKGGVGVLKVAFNEKVGSAKNELSVDGIDLTKTGLSLSDAT
                     TLDDSEATNKLTELTNALTKLRTSTSTFGSNLAIIQSRQDFTKATINTLKAGADNLVL
                     ADNNEEGATLLALQTRQQLASTALSLSNQQDQAVLRLFG"
     gene            2976..5282
                     /locus_tag="PK812_08440"
     CDS             2976..5282
                     /locus_tag="PK812_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018043505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HRJ69622.1"
                     /translation="MSKDLYSGALIYHRLPRPGKLEIQPTKPLGNQRDLALAYSPGVA
                     AACEAIKDDPAEAANLTTRGNLVAVVSNGTAVLGLGDIGPLASKPVMEGKAVLFKKFA
                     GIDVFDIEVQEKNVDKLVDVVVALEPTFGGINLEDIKAPECFEVERLCRERMNIPVFH
                     DDQHGTAIIVAAAVRNGLALVGKDIAKVKIVASGAGAAALACLNLLVSLGASRKNIWV
                     TDIEGVVYQGRKELMDPWKGKFAQKTTKRKLAEVIDGADVFLGLSAGGVLKPEMVRSM
                     AGRPLILALANPTPEIMPEEALAVRPDAMICTGRSDYPNQVNNVLCFPYIFRGALDCG
                     ATTINEEMKLAAVEAIAALAREAPSDVVARAYGGESRVFGSDMLIPSPFDPRLILRIA
                     PAVAKAAMDSGVAARPIADLSAYGESLQRFVFRSGFVMKPMFNAARANPKTVIYADGE
                     DERVLRAAQVVLEDGLARPVLVGRPGVIETRLKRFGLAIRPGRDFELVNPEDDPRYRD
                     YVQTYLDVAGRKGISPEAARTLVRTSTTVIAALALRRREVDAMICGLEGRFRSKLRLI
                     EEIIGLQPGMRDFAAMSLMITQKGHYFLADTHVQPDPSAESIAEIAIACAEHVERFGI
                     APKIALVSHSDFGAHDTPSARKMREALAIVRSRAPGLEIDGEMEGDTALVQAVRERVL
                     PSSTLKGQANILVMPNLDAANIAYQFAKVLADALPVGPILLGTALPAHVLTSSVTARG
                     VVNMTAIAVAEAQTVALAPSVAADLAAG"
     gene            complement(5413..6726)
                     /locus_tag="PK812_08445"
     CDS             complement(5413..6726)
                     /locus_tag="PK812_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69623.1"
                     /translation="MARRLLLRLQWNLFCLAVLLLASSLDVLPSAEAASLRPPSTQRL
                     RACIVPQQPELTPIFAKPPVAANDDEDDDDEDDDDDDDDEDDEDFDEEAVRGWLAPDG
                     VTCIAFGGSINAGVTSDSFALQKRGVARLRGQDVITFPLTSTARLDAMTNADGWNIAT
                     GLTIARRSGDDFVLDRATISVGPLKVGRDVSTFVFFDGADFAFSARTPARQATMLAYT
                     LQLTETLSATASAENPPDAATALAAGAATATGARWPDLIGRVLYEGDTLTLHASGALR
                     ELRRADGRPTRLASAFLLGASRDFTIGSVTHTLLAQAGGTLGGPVFIGSQLDSTDALN
                     LLTGGEIANGWSALTSLTSAWTDKVSTSAYASRYALRVPSRTSTTGLIDVSRYAMNVV
                     WKPALMVKVGLEVGWSHSDITLPRIAMNGASTQRTTATIWLQKSF"
     gene            6967..7620
                     /locus_tag="PK812_08450"
     CDS             6967..7620
                     /locus_tag="PK812_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRJ69624.1"
                     /translation="MARLSRIVIPQVPHHVTQRGNRRAPIFFEPGDQALYLDLMAERC
                     RKAGVACWAWCLMPNHVHLILTPPDADSLSRAVGEAHRRYTGFVNARARQTGHLFQSR
                     FASVAMDDAHLLAAVRYVSLNPVRAGLVRQAQDWEWSSVRAHLAGRDDRLVRVRPVLE
                     RVGAFTELLEEDAPGFAALRKAETTGRPLGSETFLARAERLLGRPLAPGKRGPKPKG"
BASE COUNT         1503 a         2683 c         2715 g         1389 t
ORIGIN      
        1 gaggcgagaa atggattctc ggaccttgtc gtagtagcgc ccattcttgg cccatcctgt
       61 ccggcaccat ggatttcggc atggggcact ggacctcctt cgcgcaggtt ctggtttcgc
      121 ggctgggcgt tcccatccat gccatccgcc tggcgcaggg cgacagcgac cggctgaacg
      181 tcggcggcgg ttcgggcggg tcgaagtcga tgcaggtggc gggcgaggca atcgtgcagg
      241 ccagcgccgt ggtcatcgag aaaggccgca ttctggcttc gcacctgttc gaggctggcg
      301 tcgacgacat cgccttcgcc aacggcgtgt tccgcgtcgc cggaacggac cgcggcatcg
      361 ccattctcga tctggcggca cgcctgcgcg ccggcacgct gcgcctgccc gaggatgcgc
      421 cgaagacgct cgatgccgat gcggtcatcg acggtgtgcc gatgacgttt ccgaacggat
      481 gccacgtggc cgaggtcgag atcgacccgg aaaccggcgc cacccgcgtg gcgcgttaca
      541 ccgccgtcac cgactccggg accgtggtca atccgatgct gctcgagggg caggtgcatg
      601 gcggggtcgt gcagggcatc ggccaggcgc tgatggaaca caccgtcttc gacgagcatg
      661 gccagctcct gaccggctcc ttcatggatt actgcctgcc gcgcgccgac accgtgccgt
      721 tcatcgccag cgaaagcctg ccgtcccctg ccaggagcaa cacgctcggc atcaagggcg
      781 tgggcgaatc gggctgcgcc ggggcgctga cctcggtgat gaacgccgtc gtggatgcgc
      841 tgtcggtcta cggcatccgc catctcgaca tgcccgcaac gccggagcgc gtcttcgcgg
      901 cgatccgggc cgcccgcgac ggcaggagct gaagcgaaac gtcgccctgc ctcagggcgc
      961 ccaataatac aactgcaaat tgttctttac aaatctacaa ccatagacaa agttcgtttc
     1021 aggattgcag gccgcgcgat caaagaccgt gggtgggcga catgaaggat agtttgggtg
     1081 catcaggtgc tgacttgggc gtcaggacga tcatcgccca gctggaggcg gcgtcaaccc
     1141 tgctgaagtt tgcggcagat gacctcgagt cctatgcgga tcgggccgat acgggagtac
     1201 gcgactcctt gcgcggaatc gtccgtgcgc tgcagaaaaa cgccgcttcc ctgttcgaga
     1261 atgcgcttta tctcgaccgg gtcgagcaaa gggatgacga ggacgagatt gccggctggc
     1321 ccctgcccag tcccgcgagg cgcgcgagcc aggcctagcg aaagacacgg caaagttgtg
     1381 taaagactcg caatgcacgc ataacgttta attaggactt gaacggcaag gtggcgaccg
     1441 acgagacatg cacgtctcgg ttgtaggtcc cactgatagg tgtgttcatg tccagtgttt
     1501 cgttctccaa ggccgtgcgc cagaacctga tctcgctgca ggagacggcg gaaaagattt
     1561 ctgtcgcgca gaatcgcctt tcgaccggaa agaaggtcaa ctcggcgctc gacaacgcca
     1621 cgaacttctt caccgccgcg ggcctcaaca gccgcgccaa cgatctttcg ctgctgctcg
     1681 acggcatctc caacgcggtg aagacggtgg atgcggccaa caacggcatc acctcgatca
     1741 cccgcctcgt ggaatcggcc cagtccaccg cgcgccaggc gctctcggac aagtcgtccg
     1801 ccagttacaa ctcgctggaa ggcgccgcca cgatcaacct gaccggttcc accggcacga
     1861 ccgccaaaga ccaggggatg aatcagacgc tcgccaacgt cggcttcgcc gatggcgata
     1921 cgttctcgct gtccaccacc aagaacggcg tcaccaagtc gttctcctac gatctgacca
     1981 ccgtcggcac caagaaggtg tccgatctcg tcgccgctat caacgcttcg ggcgtcgcga
     2041 cggcggaagt caccgatgcc ggcaagctga agatttccgg cgccgcggac gagcaggtcg
     2101 cgctccagct gaactcggcc gacggcaaca ccactgccgg aaacgcggac gttgcgaccg
     2161 gcctcggctt cacccagtcc accggcggca cgacaacggt caccaacgcc gaaggcgctg
     2221 ccaacggcga tcgcgactcg acgctgatca cacttgcggc tggcacggcc tcggtgaacc
     2281 gtgcggcgct ggtgacgcag tacaacgaga tcctcaagca ggtgaacaag ctcgcggatg
     2341 acgccagttt caacggcgtc aacctgctga agggcggcgt cggcgtgctc aaggtcgcct
     2401 tcaacgagaa ggtcggctcg gccaagaacg aactgtcggt ggatggcatc gacctgacca
     2461 agaccgggct gagcctctcc gacgccacga cgctggacga cagcgaggcc accaacaagc
     2521 tgaccgaact gaccaacgcg ctgaccaagc tgcgcacctc gacctcgacc ttcggctcga
     2581 acctggcgat catccagtcg cgtcaggact tcaccaaggc gacgatcaac acgctgaagg
     2641 cgggtgcgga caatctggtg ctcgccgaca acaacgagga gggcgccacg ctgctcgcgc
     2701 tgcagacccg ccagcagctc gcctccaccg cgctgtcgct gtcgaaccag caggatcagg
     2761 cggtgctgcg cctgttcggc taagcgcctc gccggtagca aggcggcgtc ttctcccact
     2821 cgggacgccg tcggcgggcg gtcgggcaac cggccgcccg ttaccatttc atgatcaatt
     2881 cgcatgctgc ctctgccatt cccgtggttc ccgttcgaca tcggcgaggc tatgagccat
     2941 gctcaagaaa atggccgtgg ggagacagca ccggcatgag caaggacctg tattcgggcg
     3001 cgctgatcta tcatcgcctg ccgcgaccgg gaaaactgga aatccagcca accaagccgc
     3061 tcggcaacca gcgcgatctg gcgctggcct attcgccggg cgtggcggcg gcctgcgagg
     3121 cgatcaagga tgatccggcc gaggccgcca acctgacgac gcgcggcaat ctggtggcgg
     3181 tggtgtccaa cggcacggcg gttctggggc ttggcgacat cggcccgctt gcctccaagc
     3241 cggtgatgga aggcaaggcc gtcctgttca agaagttcgc cggcatcgat gtgttcgaca
     3301 tcgaggtgca ggagaagaac gtcgacaagc tggttgacgt ggtggtggcg ctggagccga
     3361 ccttcggcgg catcaacctt gaggacatca aggcgccgga gtgctttgag gtcgaacggc
     3421 tctgccgcga gcgcatgaac attccggtgt tccatgacga ccagcacgga acggccatca
     3481 tcgtcgcggc ggccgtgcgc aacggtctgg cgctggtcgg caaggatatc gccaaggtca
     3541 agatcgtcgc ctcgggcgcc ggcgcggcgg cgctggcctg cctcaacctg ctcgtctcgc
     3601 tcggtgccag ccgcaagaat atctgggtca ccgacatcga gggcgtcgtc tatcagggcc
     3661 gcaaggagct gatggacccg tggaagggaa agttcgccca gaagacgacg aagcgaaagc
     3721 tcgcagaggt catcgacggg gcggacgtgt tcctcggtct ctccgccggt ggtgtgctca
     3781 agccggagat ggtgcgctct atggcagggc gtccgctcat cctggcgctt gccaatccga
     3841 cgccggagat catgccggag gaggcgcttg ccgtgcgtcc ggatgccatg atctgcaccg
     3901 gccgttcgga ttaccccaat caggtcaaca acgtcctgtg tttcccctac atcttccgtg
     3961 gcgcgctcga ctgcggcgcc accaccatca acgaggagat gaagctcgcg gccgtcgagg
     4021 cgatcgcggc gctggcgcgg gaggcgcctt cggatgtggt ggcgcgggcc tatggcgggg
     4081 aatcgcgcgt gttcggctcg gacatgctca ttccctcgcc cttcgatccg cgactgatcc
     4141 tgcgcatcgc gccggcggtg gcgaaggcgg cgatggactc gggcgtcgcg gcccggccga
     4201 tcgccgatct ttcggcctat ggcgagagcc tgcaacgctt cgtgttccgc tccggcttcg
     4261 tcatgaagcc gatgttcaac gcggcgcgcg ccaatcccaa gacggtgatc tacgccgacg
     4321 gcgaggacga gcgggtgctg cgcgcggcgc aggtggtgct ggaggacggg ctggcgcggc
     4381 cggtgctggt cggccgtccc ggtgtcatcg agacgcggct caagcgcttc ggccttgcca
     4441 tccgtccggg gcgcgatttc gaactggtca acccggagga cgatccgcgc taccgcgatt
     4501 acgtgcagac ctatctcgat gtcgccggcc gcaagggcat ctcgccggaa gcggcgcgca
     4561 cgctggtgcg cacctccacc acggtgattg cggcgctggc gctgcgccgg cgcgaggtcg
     4621 atgcgatgat ctgcggcctg gaagggcggt tccgctccaa gctgcggctg atcgaggaga
     4681 tcatcggcct ccagcccggc atgcgcgatt tcgccgccat gagcctgatg atcacgcaaa
     4741 aaggccatta cttcctggcc gacacccatg tgcagccgga cccgagcgcc gaatcgatcg
     4801 ccgaaatcgc catcgcctgc gccgagcatg tggaacgctt cggcatcgcg cccaagatcg
     4861 cgctggtgtc gcattccgat ttcggcgcgc atgacacgcc gtcggcgcgc aagatgcgcg
     4921 aggcgctggc gatcgtgcgc tcgcgcgcgc cggggctgga gatcgacggc gaaatggagg
     4981 gcgacaccgc gctggtccag gccgtgcgcg agcgcgtgtt gccgtcctcg acgctgaagg
     5041 ggcaggccaa cattctcgtc atgccgaacc tcgacgccgc caacatcgcc taccagttcg
     5101 ccaaggtcct ggccgatgcg cttcccgtcg ggccgatcct gctcggcacc gccctgccgg
     5161 cgcacgtgct caccagttcg gtcacggcgc gcggcgtcgt caacatgacc gcgatcgccg
     5221 tggcggaggc gcagacggta gcgctggcgc ccagcgtggc ggcggatctc gcggcggggt
     5281 agggggcggc cgccagcgcg accgcctcgt gcagcacgta ctcagatccg tcgtgagatg
     5341 tcgtgttcgt acttcaggga caggatatcc tgtcgccgcg attcaacaga attccaacag
     5401 gaaccgccaa tttcaaaacg acttttgcag ccagatggtg gctgtcgtgc gctgggttga
     5461 tgcgccattc atggcgatgc gcggcaaggt gatgtcgctg tgcgaccagc cgacctccag
     5521 cccgaccttg accatcagag cgggtttcca gaccacattc atcgcatatc gtgaaacatc
     5581 gatcagaccg gttgtggagg tgcggctggg aacacgcagg gcgtagcggc tggcataggc
     5641 gctggtggaa accttgtccg tccaggcact ggtcagcgag gtcagcgcgc tccagccatt
     5701 ggcgatctcg cctccggtca gaaggttcag cgcatcggtg ctgtcgagct gcgacccgat
     5761 gaagaccggt cctccgagcg tgccgcctgc ctgcgccagc agggtatggg tgacgctccc
     5821 gatggtgaag tcccgagaag cgcccagcag gaaggcactt gccagccgtg ttggccgacc
     5881 atccgcccgg cgcagttcgc gcagcgcgcc tgaagcgtgc agggtcaacg tgtcgccctc
     5941 gtagagcacg cgaccgatca gatcgggcca gcgcgcgcct gtcgccgtcg ccgcgccggc
     6001 cgcaagcgcc gtcgccgcgt ctggcgggtt ttcagcagat gccgtcgccg aaagagtttc
     6061 ggtcagttgc agcgtatagg ccagcatggt cgcctgccgc gccggcgtgc gggcggaaaa
     6121 cgcgaaatcc gcaccgtcga aaaacacgaa ggtggataca tcgcggccga ccttgagcgg
     6181 gccgaccgag atcgtcgccc ggtccagcac gaaatcgtcg ccggaacgcc tggcgatggt
     6241 caggccagtg gcgatattcc agccatcggc attggtcatc gcgtccagtc tggcggtgct
     6301 ggtcaggggg aaggtgatca catcctggcc gcgaagccgc gcaaccccgc gcttttgcag
     6361 cgcaaacgaa tccgaagtga cgcccgcatt gatcgatccg ccgaaggcga tgcaagttac
     6421 gccgtcgggc gccagccagc cgcgcacggc ctcctcgtcg aagtcctcgt catcctcgtc
     6481 atcgtcatcg tcatcgtcat cttcgtcatc atcatcttcg tcatcgttcg cggcgaccgg
     6541 cggcttggcg aagattggcg tcaactccgg ctgctgtggg acgatgcagg cgcgcagccg
     6601 ctgcgtcgaa ggcgggcgca gcgacgccgc ctcggcggac ggaagaacat cgagcgaaga
     6661 ggcaagcaac aggactgcaa ggcagaaaag gttccactgc aaccggagga gcaaacgacg
     6721 cgccattacg ctcaacttcc ggtggtcctg ttcgcatccg gcaaagtgcc gaccgccctg
     6781 tcaaaatgta agcccgcgag gtcgcgccct gtcaagcgaa gcggcgcggc caaagcggga
     6841 gggcccgggc aattcgggga caggcaattc ggcaattcgg ggacagaatt cggggacaag
     6901 aattcgggag aattcgggga caggatatca attgacaaaa tatgcggcgc ggcgaggatg
     6961 gcgcttatgg cccggctgtc gcgcatcgtc atcccgcagg ttccccatca tgttacgcaa
     7021 cgcggcaacc gccgggcgcc gatcttcttc gagcccggcg accaggcact ttaccttgac
     7081 ctgatggccg agcgctgccg caaggccggg gtcgcctgct gggcctggtg cctgatgccg
     7141 aaccatgtgc atctcatcct cacgcccccg gacgccgaca gtctgtcgcg cgccgtgggc
     7201 gaggcgcatc ggcgttacac cggcttcgtt aacgcacggg cgcgccagac cggacacctg
     7261 ttccagagtc ggttcgcctc cgtcgcgatg gacgatgcgc acctgctggc ggcggtgcgc
     7321 tatgtctcgc tcaaccccgt cagggcgggg ctggtgcgcc aggcgcagga ctgggaatgg
     7381 tcgagcgtgc gggcgcatct cgccgggcgc gacgaccggc tggtgcgcgt gcggccggtg
     7441 ctggagcgcg tcggagcgtt tacggaattg cttgaggaag acgcgccggg cttcgctgcg
     7501 ctgcgcaagg ccgagacgac ggggcggccg ttgggaagcg aaaccttcct cgctcgcgcc
     7561 gagcgtctgt tgggtcgccc gcttgcgccg ggaaagcgtg ggccgaaacc gaaggggtga
     7621 aggcgcggcg gactgcgaac ctccttcctg ccctcaactt attcaaccga ttttcaacct
     7681 attttgggga caggatatca attgcagaaa ttatgagaag cgcggagtgt gggattaatt
     7741 ctgtaattga tatcctgtcc ccgtaatcga ccctgtcccc gtaatcgacc accgtcaagc
     7801 gatagcgata ggaatacgtc ggcccgccgc agccgctgat cgcgagcgcg ccgatcgccc
     7861 caaggccgcc gaggaatgcg cgccgcttca tgctgcgcgc tccagagcaa gcccggcgag
     7921 aggggatgac ttatggacca ggtcctccgc ccttccggcc aagccggccc acaaacgccg
     7981 cgaaacaccc ggaaacgcgc gcaaaacgcc ccgaattcgc cggcaatcga atgcttgcac
     8041 gcactcgtgc agtgcttggc ccgccgcccc atgcgcaatc atgaacgccc cgcattgcga
     8101 tccagccgca gttaacgctg tggatttact tgtcgtcaac gggcgaattc tgggggcatt
     8161 cttgcaggcg gaggcgcgcc gcccctcacg ccgccatggc gacggcgtcc tgcagcacat
     8221 ggaccttgtt gcggccgccg gacttggcct cgcgcagcgc ctggccggcg cggcggatca
     8281 ggtcgtcgag
//
LOCUS       DAOMXN010000105         8110 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_4637_length_8110_cov_11.281316, whole genome shotgun sequence.
ACCESSION   DAOMXN010000105 DAOMXN010000000
VERSION     DAOMXN010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 8110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8110
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4637_length_8110_cov_11.281316"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1001
                     /locus_tag="PK812_08455"
     CDS             <1..1001
                     /locus_tag="PK812_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HRJ69625.1"
                     /translation="ARLGIHAHDDTGQAVANSLAALMAGARQIQGTLNGLGERCGNAN
                     LVTLIGTLKLKPAFAGRFETGVSDAALAELTAVSRAFDELLNRQPRRQAPYVGASAFA
                     TKAGIHASAVLKDPKTYEHVPPEAVGNRRLVLVSTQAGKSNVLSELERLGIAVEKSDP
                     RVARLLDEVKEREALGYSYEGADASFYILAQRVLGRVPEYFKIERFKVNVERRYNADG
                     KLVSFSEAVVKVRVGERLLMSVAEGDGPVNALDRALRKDLGRYQKHIAGLQLVDFRVR
                     IFDGGTDAVTRVLIESQDETGETWTTVGVSPNIIDASFQALTDSIVYKLMRDGVGA"
     gene            1043..1957
                     /locus_tag="PK812_08460"
     CDS             1043..1957
                     /locus_tag="PK812_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018428885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRJ69626.1"
                     /translation="MPVPRIRLHASGPELSRLVAGAWRFLRGEVTTTPKELAGFIEGC
                     LALGITSFDHADVYGGYAMEALFGAALKEWGGRRSQIEIVTKCGIRAVNPRWPETRIK
                     HYDTSAAHIRSAVDRSLANFGTDYIDLLLIHRPDPFMDAADCARGLEEVVRAGKVRAV
                     GVSNFAPSQVRLLQSALSLPLVTNQIEYSVLWPDPNFDGTLDQAQELKAAPMIWSPLG
                     GGKLFSDEPSALRVRPVLEALRAKHGAEDIGSIALAWLLMHPSRPVPVLGSTRIDRIA
                     AATGALDVRLDRQDWFAIHEAAMGEPVP"
     gene            2147..2611
                     /gene="rplM"
                     /locus_tag="PK812_08465"
     CDS             2147..2611
                     /gene="rplM"
                     /locus_tag="PK812_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012170717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HRJ69627.1"
                     /translation="MKTYSAKTAEVEKKWLLIDAKGLVVGRLASIIAMRLRGKHKATF
                     TPHIDCGDNVIVINADKVVLTGRKRDQKMYYHHTGYIGGIKERSARFILEGRFPERIV
                     EKAVERMLPRGPLARKQLGNLRVYKGDKHPHEAQQPVTLDVASLNSKNARLG"
     gene            2614..3090
                     /gene="rpsI"
                     /locus_tag="PK812_08470"
     CDS             2614..3090
                     /gene="rpsI"
                     /locus_tag="PK812_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HRJ69628.1"
                     /translation="MATLQSLSDLGQAAKPAAPEAPVHVQKLDKQGRAYATGKRKDAV
                     ARVWIKPGKGKIVVNGRDLEVYFARPVLRMLLQQPLGVANRNGQYDVTVNVDGGGLSG
                     QAGAVRHGLAKALTYFEPELRGPLKKAGFLTRDSRVVERKKYGHKKARRSFQFSKR"
     gene            complement(3149..3667)
                     /locus_tag="PK812_08475"
     CDS             complement(3149..3667)
                     /locus_tag="PK812_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69629.1"
                     /translation="MSAMYSVWLMPDSASLARLQPIHERLCRQFGTPRFDLHLTLLGG
                     CALDLARLKAILPAIVENTPPFRADVIDIGTSATFFQSFYALFAAQGPILMLKERTMR
                     LALDQGVGAFMPHVSLLYGPVEAGAKAEAAAAARREANGVSIRFDSVTLAYSSDTLPI
                     ADWRIEARAPLR"
     gene            3871..4872
                     /locus_tag="PK812_08480"
     CDS             3871..4872
                     /locus_tag="PK812_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006471461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ69630.1"
                     /translation="MSSIVVERVSKSYGTAQALREVSFTVEKGSLLVLLGPSGCGKST
                     TLRLIAGLEEPTTGRIEIDGRDVTHLPPSDRRIAMVFQSYALFPHLSVAENIVFGLKV
                     RKVARGERDARLKSVADLVNLTPYLERKPAQLSGGQRQRVALARAIIAEQPVCLMDEP
                     LSNLDAQLRHEMRVEIRSLQQRLGMTMVYVTHDQTEAMTMADTVILMRDGAIEQAGAP
                     DALYNAPETTFVARFIGAPPMNLIADRGRLIGIRPEMMRLGVPGAGEVGRQGRVAAVE
                     YLGADTIVTLAIEDGERFAVRTPGVAGVRVGETAGAAWAESAQRLFDAQTGRVLAPV"
     gene            4942..6234
                     /locus_tag="PK812_08485"
     CDS             4942..6234
                     /locus_tag="PK812_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRJ69631.1"
                     /translation="MKLTRRMFSAAALALGLSASGQALAQAQTEVRFYFPVAVGGPVT
                     KIIDDYAAEFMKENPGIKVTPIYSGDYVQTVAKALTAIKGGDIPETAILLAADLFLLL
                     GEDVIVPVSDVAASADDKKWLDGFFPAFMENSKYKGKIYSVPFQRSTPVLYWNKDAFK
                     AAGLDPEKGPSNWAEMREMAKKLTKKDASGAVSQWGIQIPSDGNSSWLFSGMTTGNGV
                     RMFNADGNQVSFDDPRVIEAVNNWYALSNEDGSQPKGLISWGATPRDFIEGKAAMMWT
                     TTGNLTNVRNNAKFPFGVAFLPGMKQAGAPTGGGNFYMFKGISKAKQEATLKFMKFMT
                     TPERAADWSVKTGYVAVSPAAYETKTLKDYTASFPPAAVARDQLKHAIPELSVYENQR
                     VMKILNDAVQAVMTGAKPADVALKAAQQDAMRVLKDFK"
     gene            6436..7314
                     /locus_tag="PK812_08490"
     CDS             6436..7314
                     /locus_tag="PK812_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRJ69632.1"
                     /translation="MKLSTRTTIHAWLMVLPACALLAMFTHIPIVTTIWHSFHSTPKP
                     RRPSRFVGLDNYRDMLADPIFWKSLVNNLVYALTSVPISVALALTMALFVNSKLRGKG
                     FLRMAYFTPTVLPMIAVANIWLFFYNPQYGLLAAALKPFGITGINWLGSQDTALWALI
                     AVAIWKEAGFFMIFYLAALQQIPTDLREAAAIEGASRWTYFWRITFPLLMPTTLFVLV
                     NAVINAFRLVDHIFVMTGGGPDNATSVLLFQIYVAGFKFFDQAQAATLSVVLLAILFL
                     IAIGQFMFADKKVHYQ"
     gene            7311..>8110
                     /locus_tag="PK812_08495"
     CDS             7311..>8110
                     /locus_tag="PK812_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008034133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRJ69633.1"
                     /translation="MSPIDRQGPVSETLETVAAWMLGLLWLLPLAFAVWTAFHPSAFV
                     AKFDLFAPLTLKNFEDAWAAAPFARYFLNTTILVIGILVAQFTLCTLAAFAFARFEFW
                     GKDVLFLLILVQLLVAPEVLMLENYITIRSLGLVDTLAGIALPYLGSAFGIFLLRQTF
                     KSVPKELEEAARVEGCSSLGVLWRVYVPAAKPIYLAYGLVMVSFHWNNYVWPLIITNS
                     VANRPLTVGLAVFASVDQGIDWSIICAATLMTSGPLLVAFLLFQRQFV"
BASE COUNT         1392 a         2707 c         2619 g         1392 t
ORIGIN      
        1 gggcgcggct cggcatccac gcccatgacg acacggggca ggccgtcgcc aattcgctcg
       61 cggctctcat ggccggggcg cgccagatcc agggaacgct caacggcctg ggcgagcgct
      121 gcggcaacgc caatctggtg acgctgatcg gcacgctgaa gctcaagccg gccttcgccg
      181 gacgcttcga gaccggggtg agcgatgccg cgctggcgga actgacggcg gtctcccgtg
      241 ccttcgacga gttgctgaac cgtcagccgc gccggcaggc gccctacgtc ggggcttcgg
      301 ccttcgcgac gaaggcggga attcatgcct cggcggttct gaaggacccg aagacctacg
      361 agcatgtgcc gccggaagcg gtgggtaacc ggcgcctcgt gctggtttcg acgcaggccg
      421 gcaagtccaa tgtgctgtcg gagctggagc gccttggcat cgcggtcgag aagtccgatc
      481 cgcgcgttgc gcgcctcctc gacgaggtga aggagcgcga ggcgctcggc tattcctacg
      541 agggcgcgga cgcgtccttc tacatcctgg cgcagcgggt gctcggccgc gtgccggagt
      601 atttcaagat cgagcggttc aaggtgaacg tcgagcgccg ctacaacgcc gacggcaagc
      661 tcgtgagctt ttcggaagcg gtcgtgaagg tgcgcgtcgg cgagcggctg ctgatgtcgg
      721 tcgccgaggg cgacggcccg gtgaatgcgc tcgatcgcgc gttgcgcaag gatctcggcc
      781 gttaccagaa gcacatcgct ggcctgcaac tcgtcgactt ccgcgtgcgc atcttcgacg
      841 gcggcaccga cgcggtgacg cgcgtgctga ttgaatcgca ggatgaaacc ggcgaaacct
      901 ggaccacggt cggcgtctcg cccaacatca tcgacgcctc gttccaggcg ctgacggatt
      961 ccatcgtcta caagctgatg cgcgacggcg tgggggcgta ggggcctgat ccaggtttca
     1021 tcttgcaagc agagtgagcg acatgcccgt tccccgcatc agacttcatg ccagcggccc
     1081 ggaactctcg cggctcgtcg ccggggcctg gcgcttcctg cgcggcgagg tgaccacgac
     1141 gccgaaggaa ctcgccgggt tcatcgaggg ctgcctcgcg ctcggcatca ccagtttcga
     1201 tcatgccgac gtctatggcg gctatgcgat ggaagcgctg ttcggcgccg cgctgaagga
     1261 atggggcggc aggcgctcgc aaatcgaaat cgtcaccaag tgcggcatcc gcgcggtgaa
     1321 cccgcgttgg cctgagacac gcatcaagca ctatgacacc tcggccgccc acatccggtc
     1381 ggcggtggac cgctcgctgg cgaactttgg caccgactat atcgatcttc tgctgatcca
     1441 ccggccggac ccgttcatgg acgcggccga ctgcgcgcgc ggtctggagg aggtggtgcg
     1501 ggccggcaag gtgcgtgccg tcggcgtatc gaatttcgct ccctcgcagg tgaggctgct
     1561 gcaatcggcg ctgtcgctgc cgctggtgac gaaccagatc gagtattccg tgctctggcc
     1621 ggatccgaat ttcgacggca cgctggatca ggcgcaggaa ttgaaggccg cgccgatgat
     1681 ctggtcgccg cttggcgggg gcaaactgtt ttccgacgag ccgtccgccc tgcgcgtgcg
     1741 cccggtgctg gaggcgctcc gcgcgaaaca cggagcggaa gacatcggct ccatcgcgct
     1801 cgcctggctg ctgatgcatc caagccggcc cgtgccggtt ctcggttcga cgcgcattga
     1861 ccggatcgcg gcggcgacgg gcgcgctgga cgtcaggctc gaccggcagg actggttcgc
     1921 catccacgag gcagccatgg gggagccggt tccgtgaacc catgggaaga cgaatgaggt
     1981 aaaataatac cgtttttcct aggcacttga aaaatataga tatttgcaga agccccgcga
     2041 tccttgtgct cttgacgcca caaaatcaat cgcttagagc cagcaccacg cgagcggtcc
     2101 cgtccggggc cgctttcccg tttcgtccta gttcaggatt tccctcatga agacctattc
     2161 ggccaagacc gccgaggtcg agaagaaatg gctgctgatc gacgcgaaag gtctcgtcgt
     2221 cggccgtctt gcctcgatca tcgccatgcg tctgcgcggc aagcacaagg cgacgttcac
     2281 cccgcacatc gactgcggcg acaatgtcat cgtcatcaac gccgacaagg tggtcctgac
     2341 cggccgcaag cgcgaccaga agatgtacta ccaccacacc ggctacatcg gcggcatcaa
     2401 ggagcgctcg gcgcgcttca tcctggaagg ccgtttcccg gagcgcatcg tcgagaaggc
     2461 cgtggagcgc atgctcccgc gcggtccgct cgcccgcaag cagctcggca acctgcgcgt
     2521 ctacaagggt gacaagcatc cgcacgaggc gcagcagccg gtgacgctcg acgtcgcctc
     2581 cctcaactcc aagaatgcga ggctcggctg atcatggcga cccttcagtc tctctccgac
     2641 ctcggacagg ccgccaagcc ggccgcgccg gaagcccccg ttcatgtcca gaagctggac
     2701 aagcagggcc gtgcctacgc gacgggcaag cgcaaggacg ccgtcgcccg cgtgtggatc
     2761 aagccgggca agggcaagat cgtcgtcaac gggcgcgatc tggaagtcta cttcgcccgt
     2821 ccggtgctgc gcatgctgct ccagcagccg ctcggcgtcg ccaaccgcaa cggccagtat
     2881 gacgtcacgg tcaacgtcga cggcggcggc ctttcgggcc aggccggcgc ggtgcgtcac
     2941 ggcctcgcca aggcgctgac ctacttcgag ccggaactgc gcggcccgct gaagaaggct
     3001 ggcttcctca cccgcgacag ccgcgtggtc gagcgcaaga agtacggcca caagaaggcc
     3061 cgccgcagct tccagttctc gaagcgctaa aggcgcgcat tcccgcaaag tgtggcaagg
     3121 cccggagcga tccgggcctt ttcgtttgtc accgcagcgg cgcgcgcgcc tcgatccgcc
     3181 agtcggcgat cggcagcgtg tcggacgaat aggcgagcgt gacggaatcg aagcggatcg
     3241 ataccccatt cgcctcgcgg cgtgccgcag cggcggcctc tgccttggcc ccggcctcga
     3301 ccgggccata cagcagcgag acatgcggca tgaaggcgcc gacgccctga tccagcgcca
     3361 ggcgcatggt gcgctccttg agcatcagga tgggcccctg cgccgcgaac agcgcataga
     3421 acgactggaa gaacgtcgcg cttgtgccaa tgtcgatgac atcggcgcga aatggcgggg
     3481 tgttctcgac gatcgccggc aggatggcct tcagccgcgc cagatccagc gcgcagccgc
     3541 cgagcagcgt caggtgcagg tcgaatcgcg gcgtgccgaa ctggcggcac agccgctcat
     3601 ggatcggctg gagccgcgcc agacttgccg aatcgggcat cagccacacc gaatacatcg
     3661 cgctcattgc aggtcctgat cgagaatgcc acgccagcgt ctccgtgccg ccgaccgagc
     3721 cggcaagaaa gccacgcaac gccacacatt acaatattgt gacaagcgtg aaggcgcact
     3781 agcatccgcg catcggttgt gaccacactg tcatgaacca gccttaggga gaaggcgcgg
     3841 caacagcgcc ggacaaatgc agggaatggc gtgtcgtcca tcgtcgtcga gcgggtgagc
     3901 aagagttacg gcacggcgca ggccctgcgc gaagtgtcgt tcacggtcga gaagggcagc
     3961 ctgctggtgc tgctcggccc ttccggctgc ggcaagtcaa cgacgctgcg cctgatcgcc
     4021 ggactggagg agccgacgac ggggcgcatc gagatcgacg ggcgcgacgt cacgcatctg
     4081 ccgccctccg accggcgcat cgccatggtg ttccagtcct atgcgctgtt cccccacctg
     4141 tccgtggccg agaacatcgt cttcgggctg aaggtgcgca aggtggcgcg cggggaacgt
     4201 gacgcgcggc tgaagtcggt cgccgacctc gtcaacctga cgccctacct cgagcgcaag
     4261 cccgcccagc tctccggcgg ccagcgccag cgcgtggcgc tggcgcgcgc catcatcgcg
     4321 gagcagccgg tgtgccttat ggacgagccg ctctcgaacc tcgacgcgca actgcgccac
     4381 gagatgcgcg tggaaatccg gtcgctccag cagcgactcg gcatgaccat ggtctatgtg
     4441 acccacgacc agaccgaggc catgaccatg gccgacaccg tcatcctgat gcgcgacggc
     4501 gcgatcgagc aggctggcgc gcccgatgcg ctctacaacg cgccggaaac gaccttcgtg
     4561 gcgcggttca tcggcgcgcc gcccatgaac ctcatcgcgg atcgcgggcg gctcatcggc
     4621 atccgtccgg agatgatgcg gctcggcgtg cccggagccg gcgaggtcgg ccggcagggc
     4681 cgcgtggcgg cggtggagta tctcggcgcc gacaccatcg tcacgctcgc gatcgaggac
     4741 ggcgaacggt ttgccgtgcg cacgccgggc gttgccggag tgagggtcgg cgagaccgcg
     4801 ggagcagcct gggccgagag tgcccaacgc ctgttcgatg cccagacggg acgggttttg
     4861 gctccggtgt gacgcaccgg ggcagggacg tgcctttttt gaaccgccgc gcgacgcggc
     4921 aagctgaagg agtgagggac gatgaaactg acacgcagga tgttctcggc cgcggcactg
     4981 gcgctcggcc tttccgcgag cggacaggcg ctcgcccagg cgcagaccga ggtgcgcttc
     5041 tatttccccg tcgccgtcgg cggtccggtc accaagatca tcgacgatta cgccgccgag
     5101 ttcatgaagg agaaccccgg gatcaaggtg acgccgatct attccggtga ctacgtgcag
     5161 accgtcgcca aggcgctgac ggccatcaag ggcggcgaca ttccggagac ggcgatcctg
     5221 ctcgccgcgg acctgttcct cttgctcggc gaggacgtga tcgtgccggt tagcgatgtc
     5281 gccgcgtcgg cggacgacaa gaaatggctg gatggcttct tcccggcctt catggagaac
     5341 tcgaagtaca agggcaagat ctactccgtg ccgttccagc gctcgactcc ggtcctctac
     5401 tggaacaagg acgccttcaa ggccgccggg ctcgacccag aaaaaggtcc ttccaactgg
     5461 gccgaaatgc gcgagatggc caagaagctg acgaagaagg atgcgtccgg cgcggtcagc
     5521 cagtggggca tccagatccc ctcggacggc aattcctcct ggctgttctc cggcatgacc
     5581 accggcaacg gcgtccgcat gttcaacgcc gacggcaacc aggtgtcctt cgatgatccc
     5641 cgggtgatcg aggccgtgaa caactggtac gcgctgtcca acgaggacgg ctcgcagccc
     5701 aagggcctca tttcctgggg cgcgacgccg cgcgacttca tcgagggcaa ggccgcgatg
     5761 atgtggacca ccaccggcaa cctcaccaat gtccgcaaca acgccaagtt ccccttcggc
     5821 gtcgccttcc ttccgggcat gaagcaggcg ggtgccccga cgggcggcgg caacttctac
     5881 atgttcaagg gcatctccaa ggcgaagcag gaggccaccc tcaagttcat gaagttcatg
     5941 accacgccgg agcgcgcggc cgactggtcg gtgaagaccg gctatgtcgc ggtctcgccg
     6001 gctgcctacg agaccaagac gctcaaggac tacaccgcct ccttcccgcc ggcggcggtg
     6061 gcgcgcgacc agctcaagca cgcgattccg gagctctcgg tctatgagaa ccagcgcgtg
     6121 atgaagatcc tgaatgacgc cgttcaggcc gtcatgaccg gcgccaagcc ggcggacgtc
     6181 gcgctcaagg cggcccagca ggacgccatg cgcgtgctga aggacttcaa gtgacgctgc
     6241 accggcgccc ggtgcctgaa cggctggaag catccctctc cccttgcggg agagggtggc
     6301 ccaagcgtgc cctggcgcgc ggtgcgtcgg gtgagggggc ggcgcaattt cccctcatcc
     6361 gtcggcttcg ccgccacctt cccccgcaag ggaagaaggc agaaaggcgc gccgcatgag
     6421 ccttgcgatg ttccgatgaa actctcgacc cgcaccacga tccacgcctg gctgatggtg
     6481 ctgcccgcct gcgcgctgct ggcgatgttc acgcatatcc cgatcgtcac gacgatctgg
     6541 cacagctttc actccacgcc caagccgcgc cgtccctcgc gcttcgtcgg cctcgacaac
     6601 taccgcgaca tgctggccga cccgattttc tggaagtcgc tggtcaacaa cctggtctat
     6661 gccctgacct cggtgcccat ctcggtggcg ctggcgctga ccatggcgct gttcgtgaat
     6721 tcgaagctgc gcggcaaggg cttcctgcgc atggcctatt tcacgccgac cgtgctgccc
     6781 atgatcgccg tcgccaacat ctggctgttc ttctacaacc cgcagtacgg cctgctggcc
     6841 gccgctctca agcccttcgg catcaccggc atcaactggc tcggctcgca ggacaccgcg
     6901 ctctgggcgc tgatcgccgt ggcgatctgg aaggaggccg gcttcttcat gatcttctac
     6961 ctcgcggcac tccagcagat tccgaccgac ctgcgcgagg ccgccgccat cgagggcgcg
     7021 agccgctgga cctatttctg gcgcatcacc ttcccgctgc tgatgccgac cacgctgttc
     7081 gtgctggtca acgcggtgat caacgccttc cgccttgtgg accacatctt cgtcatgacc
     7141 ggcggcgggc cggacaacgc cacctcggtg ctgttgttcc agatctatgt cgcgggcttc
     7201 aagttcttcg accaggcgca ggcggcgacg ctctccgtcg tgctgctggc gatcctattc
     7261 ctcattgcca tcgggcagtt catgttcgcc gacaagaagg ttcactacca atgagcccga
     7321 tcgaccggca ggggccggta tccgagacgc tcgaaacggt ggcggcctgg atgctcgggc
     7381 tgctgtggct gctgccgctg gccttcgcgg tgtggacggc cttccacccc tcggccttcg
     7441 ttgcgaagtt tgatctcttc gcaccgctta ccctgaagaa cttcgaggac gcctgggccg
     7501 ccgcgccctt tgcccggtac ttcctcaaca ccacgatcct ggtgatcggc attctcgtgg
     7561 cgcagttcac gctctgcacc ctcgccgcct tcgcctttgc ccgcttcgag ttctggggaa
     7621 aggacgtgct gttcctgctc atcctggtgc agttgctggt cgcgccggaa gtgctgatgc
     7681 tggagaacta catcaccatc cgctcgctcg ggctggtgga cacgctggcc ggcatcgcgc
     7741 tgccctatct cggctcagcc ttcggcatct tcctcctgcg ccagaccttc aagtccgtgc
     7801 cgaaggaact ggaggaggcg gcgcgcgtcg agggctgctc gtcgctcggc gtgctctggc
     7861 gcgtctatgt tccggcggca aagcccatct acctcgccta cgggctggtg atggtctcgt
     7921 tccactggaa caattacgtc tggccgctga tcatcaccaa ctcggtggcc aaccggccgc
     7981 tgacggtggg gctggccgtc ttcgcctccg tggatcaggg catcgactgg tcgatcatct
     8041 gcgccgcgac gctcatgacc tccggcccgc tgctggtggc gttcctcctg ttccagcgcc
     8101 agttcgtgca
//
LOCUS       DAOMXN010000106         8103 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_4640_length_8103_cov_10.739190, whole genome shotgun sequence.
ACCESSION   DAOMXN010000106 DAOMXN010000000
VERSION     DAOMXN010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 8103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8103
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4640_length_8103_cov_10.739190"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="PK812_08500"
     CDS             <1..415
                     /locus_tag="PK812_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007009587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRJ69634.1"
                     /translation="GAAFDRGVYYLPTIAEGLDNRSRLAREEVFGPVLLVLPFDNEDT
                     LVEEANDSLYGLAAGIWTRDFNKAWRLARRLEVGTVWINTYKQFSIATPFGGLKQSGI
                     GREKGLDGLRAYMSQKSIYWDTSGVPFPWAGGAVP"
     gene            412..1668
                     /locus_tag="PK812_08505"
     CDS             412..1668
                     /locus_tag="PK812_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008597311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRJ69635.1"
                     /translation="MSAPSIAIVGAGPAGLFTAQALRRALPTAPITVIDRLPVPFGLL
                     RYGVAPDHQGTKAIARQFERLFERENVRFCGGIEVGADLPLETLRRGHTIVVLAAGLQ
                     GDRPLAIPGDDLPGIIGSGRLTRWFNDHPDEAGFDPPIGRRVAIIGNGNVALDIARIF
                     AKSEAEFAGSDLSPRTLARISAAGVEEIVVIGRGQADAARFDPALLKEFTRLGETHVK
                     AEVAGSEASQSPVATALREIDGTGALPGNARRRLTFRFGLTPKHIRSADGALVLHLAR
                     TSGDGNEAAIATDTVITAIGFDAGRTGLPRTEWLQGDGTLAVRIAPGLYASGWFRRGP
                     RGTIPDNRAEAREVADRIVADVSCGAVPAVSTDIQADLFARAVSYAGWTAIRDREIAE
                     APAGRVRIKCPTAAQMRAIAAGAPAP"
     gene            1665..2102
                     /locus_tag="PK812_08510"
     CDS             1665..2102
                     /locus_tag="PK812_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HRJ69636.1"
                     /translation="MKPLILYGTETGNAEMLAEDIASHLGDAQLLNMSDATVAALTAA
                     GLLIVVCSTYGEGELPSGARALHAAVEAARPDLSNLRFAAFSLGDKATYPDTFARGGA
                     IWAETLSALGARQLGEIATHDAAGADLAEEIALPWIDDIMAEA"
     gene            2107..2724
                     /locus_tag="PK812_08515"
     CDS             2107..2724
                     /locus_tag="PK812_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HRJ69637.1"
                     /translation="MRSLSAAEIVARAAAPLQKGVTVDLTGLDVHEPLVLTERDLANL
                     DFSGSTFHAPVRLAGARFLGLAWFGNCRFHSAFDASRAVFHNDLRAEDCLFYQDFTCA
                     RAEFRGVACFDRSDFHGPAHLDRLTCYGNLSLDHAVFRAPVSLQNSEFLGGIWCDSTE
                     FRIRADFRDVEVHGRTWVRQASVGKENAARSSSLRQISSYGLQWL"
     gene            2752..3522
                     /locus_tag="PK812_08520"
     CDS             2752..3522
                     /locus_tag="PK812_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HRJ69638.1"
                     /translation="MTDRYLVPGLQRGIDVLQAFTPERQRLSLGELAAAVGVSKSAVY
                     RVTYTLCQCGLLLQDSHDKSFTLGPAVLRLGYGYLAAREIVEVAMPHLELLRDETGWS
                     AHLGVLEGMDVLYLMRVPARRGAASIVHVGSRLPAHATAMGRILLAHQPEADLKARYV
                     GDTQQRPDARTLPALAAIQRQAAEDRARGHVAHAGTFERSTASIAAGVHDVTGRVVAA
                     INLAGAMSDAGDAIPDAVRMHLLAAAERISTVLGHKPG"
     gene            complement(3560..4507)
                     /locus_tag="PK812_08525"
     CDS             complement(3560..4507)
                     /locus_tag="PK812_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008597331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRJ69639.1"
                     /translation="MDTAYPASSALRQALRSHVVRHVDKSFDWDAFPSNRGYPDLERA
                     QMRYIGAGGSPKVGEAGTLKPEHFTVSLVYKHVGKFAACHAHEIEESFLIIQGVLTVG
                     WEQNGDVVEVRLGPKDMILNARDIGHGFRNDGVEPVLMSISVDVGKPLPPRYLFHPKS
                     TDLELARRFGAAEGKTMPFDASSAHPLQRAIAGAVIRHADAPTIWERAGFSRKVYVGP
                     GGVASTTCRKEMLGVPVGVGVKPFVRDVEDAFLVLEGTLTVGWEEGGEKVEMELGPRD
                     LVMNPAGRRHWFRNNSAGPCTVWSVVGTDRPESVVFEAA"
     gene            complement(4615..6042)
                     /locus_tag="PK812_08530"
     CDS             complement(4615..6042)
                     /locus_tag="PK812_08530"
                     /EC_number="1.2.1.68"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050269 - coniferyl-aldehyde dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006081 - cellular aldehyde metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coniferyl aldehyde dehydrogenase"
                     /protein_id="HRJ69640.1"
                     /translation="MNYHAADQTTAASLRAALEIQRRAYFAQPVPTLAERRSDLDALA
                     RFVKENRQALIEAISADYGHRSHHETLLTEIGPVLAGIRFARRNLRKWMKPQRRHVDR
                     LSYGLASNTVVPQPLGVVGVIVPWNFPLNLSLVPLTYIFAAGNRAMVKMSENSRNLAL
                     LMIERMPNYFPPEKLRFFDETGGIGIEFSKLPFDHLLFTGSGTTGRAVMAAAAQNLCP
                     VTLELGGKAPAIICDDFDLKLAAERILFVKYLNAGQICTTVDHVYVPHDKVKAFIEHA
                     RAIVPRRYAGIDSPDLTSIIDRRAYERLKQGLSEAQARGATVIALTPGKAFDDEKHRI
                     APHLVTDAPADCQLMQREIFGPVLPVIGYRDVEEVIRAINSGARPLAIYPFSNQGPVI
                     DRLIERVMSGGVTVNDALMHVGQDDLPFGGVGESGMGHYHGHEGFNTFSKLRPVFRQT
                     RFASTMLLMPPFGKLADRLLSFLTR"
     gene            complement(6134..>8103)
                     /locus_tag="PK812_08535"
     CDS             complement(6134..>8103)
                     /locus_tag="PK812_08535"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HRJ69641.1"
                     /translation="GGVLTGVAAGVAAGAALWRPAAPPKRAFTASSASDAVRQAHQAS
                     TAGLNLADESTFADARRGFIARPSGKVQSASGETIWDFDSFAFVNGAAPPTVNPSLWR
                     QARLNNEIGLFKVTEGIWQLRGFDLANMTLIEGRTGWIVVDTLTARETAATAIAFARQ
                     HLGNKPISAIIYTHSHADHFGGTLGLVSAEEVKARQIPIIAPAGFLEEATSENVLMGV
                     AMGRRSTYMYGSRLPRNPQGLIDNGLGKAVAYGSIGILPPTRIIEQRQEALDVDGVRF
                     IFHNVPESEAPSEFVFQIPSLKAFCGADLLSFTQHNLYTLRGTKVRDALKWADYIDQA
                     LSQTANADVVFNQHHWPVWGRERIRDFIVKQRDIYRYIHDQTVRLMNAGLTAPEIAET
                     LELPRSLNEHLAVRGYYGTVRHNVRAVYQYYLGWYDAHPANLDALPPVDAGKRYVALA
                     GGMDKLLAAAQSAYDRGDLRWAAELLKHAVYAEPGNKQAGELLAQTFDQLGYMAESTA
                     WRNVYLSGAYELRQGAPTQGLSLDMLKDLLSHTPIDRFLERMAASIDGIRAAEMKAKI
                     NLVLSDLRESFVLELENGVLHHRKGDPAKDADATLTVTHPFFLRMMTGKAGAMDLLTS
                     SEANIDGSTLVLGRFLMLVEKAPGIFPIVTR"
BASE COUNT         1377 a         2700 c         2569 g         1457 t
ORIGIN      
        1 gggcgcggcc ttcgaccgcg gcgtctacta tctgccgacc atcgccgagg ggctggacaa
       61 ccgctcgcgg ctggcccgcg aggaggtctt cgggccggtc ctgctggtcc tgcccttcga
      121 caatgaggac accctggtcg aggaggccaa cgacagcctt tacggtctcg ccgccggcat
      181 ctggacacgc gacttcaaca aggcctggcg gctggcgcgc agactggaag tcggcacggt
      241 ctggatcaac acctacaagc agttctccat cgcgacgccg ttcggtggcc tgaaacagag
      301 cggcatcggt cgggaaaagg gcctcgacgg tctgcgggcc tacatgagtc agaagtcgat
      361 ctactgggat accagcggcg tgccgtttcc ctgggctggg ggcgccgttc catgagcgcc
      421 ccgtcgattg ccatcgttgg cgccggaccg gcgggccttt tcaccgccca ggccctgcgc
      481 cgcgccttgc ccacggcgcc gatcacggtc attgaccggc ttccggtccc cttcggcctg
      541 ctgcgttatg gcgtggcgcc cgaccatcag ggaacgaagg ccatcgcccg ccagttcgag
      601 cgtcttttcg agcgggagaa cgtgcggttc tgcggcggca tcgaggtcgg cgccgatctg
      661 cccctggaga cgctgcgacg cggccacacc atcgtcgtgc tggccgccgg tctgcagggt
      721 gaccgcccgc ttgccatccc cggggatgac ctgccgggca tcatcggttc cgggcgattg
      781 acccgctggt tcaacgatca ccccgatgag gccggtttcg atccgccgat cggccgccgc
      841 gtcgccatca ttggcaatgg caacgttgcg ctcgacatcg cccgcatctt cgccaagtcc
      901 gaagccgagt tcgcgggctc cgacctttcg ccgcgcacgc tggcgcgaat ttcagcggct
      961 ggcgtcgagg agatcgtcgt catcggtcgc ggacaggcgg atgcggcgcg cttcgatccg
     1021 gcactgctga aggaattcac ccgcctcggc gaaacgcatg tgaaggccga agtcgcgggc
     1081 agcgaagcgt cgcagagccc ggtcgccacc gcgctgcgtg agatcgacgg taccggcgcc
     1141 ctgcccggga acgcccgccg acgcctgacc ttccgcttcg gcctgacgcc gaaacacatt
     1201 cgcagcgccg acggcgcgct ggttctgcac ttggcccgga caagcggcga tggcaacgag
     1261 gccgcgatcg cgacggatac cgtcatcacc gccataggct ttgacgcggg ccgcaccggt
     1321 ctgcctcgca ccgagtggtt gcagggcgat ggcacgcttg cggtccgtat cgcgccgggc
     1381 ctctatgcct cgggctggtt ccgtcgcgga ccgcgcggca cgatcccgga caaccgggcc
     1441 gaagccaggg aggttgctga ccggatcgtc gccgatgtgt cgtgcggcgc ggttccggcc
     1501 gtttccaccg acattcaggc cgatctgttc gcccgcgccg tgtcctacgc cggctggacc
     1561 gccatccgcg atcgcgagat cgccgaagcg ccggccggac gcgttcgcat caaatgtcca
     1621 accgctgcgc agatgcgcgc catagccgca ggagcgcctg ccccatgaaa ccgctgatcc
     1681 tctatggcac cgaaaccggc aatgccgaga tgctggcgga agacatcgcc agccatctcg
     1741 gcgatgcgca gcttctgaac atgagcgatg cgacggtggc ggcgctgacc gccgccggtc
     1801 tcctgatcgt ggtgtgctcg acctacggcg aaggggaact gccgagcggc gcgcgcgcgc
     1861 tccacgccgc agtcgaagcg gcacggcccg atctgtcaaa cctgcgtttc gccgccttca
     1921 gcctcggcga caaggcgacc tatcccgata ccttcgcccg aggcggcgcg atctgggccg
     1981 agaccctgag cgcactcggc gcccggcagc tcggcgagat cgcgacccat gatgctgccg
     2041 gcgccgacct cgcggaggag atcgccctgc cctggatcga cgacatcatg gcggaagcct
     2101 gagcccatgc gctccctttc cgccgccgaa atcgttgccc gtgcggcagc cccgctccag
     2161 aagggcgtca cggtcgactt gaccggactg gatgttcatg agccgctcgt gctgaccgag
     2221 cgggacctcg ccaatctcga cttctccggc agcaccttcc atgccccggt gcgtctcgcg
     2281 ggcgcgcgct ttctcggtct cgcctggttt ggaaactgcc gattccattc cgccttcgat
     2341 gcgtcgcggg ccgtgtttca caacgatttg cgtgccgagg attgcctttt ctaccaggat
     2401 ttcacctgcg cgcgggccga gtttcgcggc gtcgcctgct tcgaccggtc ggatttccat
     2461 ggcccggccc atctcgacag gctgacctgc tatggcaacc tgtccctcga tcacgccgtg
     2521 tttcgagcgc ccgtttcgtt gcagaattcg gagtttctgg gcggaatttg gtgcgattcg
     2581 acggagtttc gcatccgcgc cgatttccga gacgtcgagg tccacgggcg aacctgggtc
     2641 cggcaggcat cggtcggcaa ggaaaacgcc gcgcgatcat cgagtttgcg gcaaatcagc
     2701 agttacggcc tgcaatggct ctgaaaatgg gcaagaacgg gcagaatccc gatgacggac
     2761 cgctacctcg tccctggcct gcagcgcggc attgacgttc tgcaagcctt cacgccggag
     2821 cgccagcgcc tgtcgctcgg cgaacttgcc gccgccgtcg gagtcagcaa gtcagcggtg
     2881 taccgcgtca cctacacgct ctgccagtgc ggcctgctct tgcaggattc ccatgacaag
     2941 tccttcacgc tgggaccagc ggtcttgcgg cttggctacg gctatcttgc tgcgcgcgag
     3001 atcgttgaag tggcgatgcc gcatctggaa ctgctgcgcg atgagacggg ctggtccgcc
     3061 catctcggcg tgctcgaagg catggacgtg ctctatctca tgcgcgtgcc agcccggcgc
     3121 ggcgcggcca gcattgtgca cgtcggctcc cggctgccgg cccatgcgac cgctatgggg
     3181 cgcatcctgc tcgcgcacca gccagaggcg gacctgaagg cgcgctatgt cggcgacacc
     3241 cagcagcgtc ccgatgcccg aacccttcct gcactggcag caatccagcg gcaggcagcc
     3301 gaagatcggg cacgaggtca cgttgcgcat gcgggcacat tcgagaggtc gacggccagc
     3361 attgcggcag gcgtgcacga cgtgaccggc cgcgttgtcg cggccatcaa tctggcgggc
     3421 gcgatgtcgg atgcgggcga cgccataccc gacgccgtcc gcatgcacct tctggcagcg
     3481 gccgaacgga tttccaccgt tcttggccac aaaccgggct gagcaggcgc cgcggccgtt
     3541 ccggctgccc acgaacctgt caggcggcct cgaacaccac ggactccgga cggtccgtgc
     3601 caaccacgga ccagaccgtg caaggcccgg cgctgttgtt gcggaaccag tgccggcgcc
     3661 ctgccgggtt catgacgaga tcgcgcgggc cgagttccat ctcgaccttt tcgccgccct
     3721 cttcccagcc aaccgtcagg gtgccttcga gcaccaggaa ggcatcctcg acgtcgcgaa
     3781 cgaaaggctt gaccccgacg ccaaccggca cgcccagcat ctccttgcgg caggtggtcg
     3841 aggcgacgcc gcccggccca acgtacacct tacggctgaa gcccgcccgc tcccagatcg
     3901 tcggcgcgtc ggcatggcga atgacggcgc ccgcaatggc gcgctggagc ggatgcgcgc
     3961 tcgacgcgtc gaacggcatg gtcttgccct cggccgcgcc gaaacgccgt gccagttcga
     4021 ggtccgtgct cttcgggtgg aagaggtacc ggggtggcaa gggcttgccg acatcgacgc
     4081 tgatcgacat caggaccggc tcgacaccgt cgttgcggaa gccgtggccg atgtcgcgcg
     4141 cgttgaggat catatccttg gggcccagcc tgacttcgac cacatccccg ttctgctccc
     4201 aaccaaccgt cagcacaccc tggatgatca ggaaagattc ttcgatttca tgagcatggc
     4261 aggccgcgaa cttgccaaca tgcttgtaga cgaggctcac cgtgaaatgc tcgggcttca
     4321 gcgtgccggc ctcgccaacc ttgggcgacc cacccgctcc gatgtagcgc atctgcgccc
     4381 gttccaggtc gggatacccg cggttggagg ggaacgcgtc ccagtcgaag gatttgtcga
     4441 catggcgcac gacatgactg cgcagtgcct gacgcagggc ggaactggct ggataggcgg
     4501 tgtccattgg cgatctcccc tcggctcaaa tacaatgcgg ggaacactag cctcaggcca
     4561 gccggccggc tacggcacgt tttgctggca aaacgtccgc ccttgcggat gggcttaccg
     4621 cgtgaggaac gacaggagtc ggtctgccag tttcccgaaa ggcggcatca gcagcatcgt
     4681 gctggcaaag cgggtctgac ggaacacggg ccgcaacttg gaaaaggtgt tgaacccctc
     4741 gtggccgtgg tagtgcccca tgcctgactc ccccacaccg ccgaacggca aatcgtcctg
     4801 cccgacatgc atcagggcat cgttgaccgt gacgccaccc gacatcacac gctcgatcag
     4861 ccggtcgatg accggaccct ggttgctgaa cgggtatatc gccagcggcc gcgctccgga
     4921 gttgatggct cggatcacct cctcgacatc gcggtagccg atcaccggaa ggaccggtcc
     4981 gaaaatctcg cgctgcatga gctggcagtc tgccggcgca tcggtgacca ggtgcggcgc
     5041 gatgcggtgc ttctcatcgt cgaatgcctt gcccggcgtc agggcgatca ccgtcgcgcc
     5101 gcgcgcctgc gcttccgaga gcccctgttt caggcgttca taggcgcgcc ggtcgatgat
     5161 gctggtaaga tcgggcgagt cgatgccggc gtagcgacgc ggaacgatgg cgcgggcatg
     5221 ctcgatgaac gccttgacct tgtcgtgcgg cacatacacg tgatcgacgg ttgtgcagat
     5281 ctgtccggcg ttcagatact tcacgaacag gatgcgctcg gccgccagct tgaggtcaaa
     5341 atcgtcgcag atgatcgccg gcgccttgcc gccgagttcc agggtgaccg ggcacaggtt
     5401 ctgcgccgcc gcggccatca cggcgcggcc ggttgtgccg gaaccggtga acaacaggtg
     5461 atcgaacggc aacttggaga attcgatgcc gattcctccg gtctcatcga aaaacctcag
     5521 tttttccggg ggaaagtaat tcggcatccg ctctatcatc agcaaagcaa gattgcggga
     5581 gttctcgctc atcttcacca tggcccggtt gcccgccgcg aagatgtagg tgagcggcac
     5641 caggctgaga ttgagcggaa aattccacgg cacgatgacg cccacgacac ccagcggttg
     5701 tggcaccacc gtgttgctgg ccagtccgta ggacagccga tcgacgtgtc tacgctgcgg
     5761 cttcatccac ttccgcagat tgcgccgcgc gaagcggatg ccggccagaa ccggcccgat
     5821 ctcggtcaac agcgtctcgt gatgcgaacg gtggccatag tccgccgaaa tcgcctcgat
     5881 cagggcctgt cggttttctt tgacgaaccg ggcgagcgca tcaaggtcag acctgcgttc
     5941 ggcaagggtc gggacgggct gtgcaaaata ggcccggcgt tggatttcca gcgccgcacg
     6001 caagcttgcg gcagtcgtct gatcggcagc gtgataattc atcgctcgtt cctttcgcct
     6061 gcgctctcac tcaggcctca gtgtaggacg aaacgcgtct ccgaccaacc gtttcggtac
     6121 gtttgcggca gtatcaacgc gttacaatcg gaaagatgcc cggcgccttc tcgaccagca
     6181 tgagaaatcg accgagcacc agcgtgctgc cgtcgatgtt ggcctcgctc gacgtcagca
     6241 gatccatggc tccggctttc ccagtcatca tcctcaagaa gaaggggtgc gtcaccgtca
     6301 gagttgcatc ggcatccttc gctgggtcac ccttgcggtg gtgcagcaca ccgttttcga
     6361 gttcgagaac gaagctctcg cgcaggtccg acagcaccag gttgatcttg gccttcatct
     6421 ccgccgccct gataccatcg atcgacgccg ccatgcgttc aaggaagcgg tcgatggggg
     6481 tgtggctcag caggtccttg agcatgtcca gggacagtcc ctgcgtcggc gcaccctgcc
     6541 gcaattcata agccccgctg agatagacgt tccgccaggc tgtcgattcc gccatgtatc
     6601 caagctgatc gaatgtctgc gccagcaatt ctcccgcctg cttgttgccc ggttcagcgt
     6661 agaccgcgtg tttcaagagc tccgccgccc accggaggtc tccgcgatca taggccgact
     6721 gcgccgccgc gagcagctta tccatgcctc cggccagcgc gacgtaacgt ttgccggcat
     6781 cgacgggtgg caacgcatcg aggttggccg gatgcgcatc gtaccagccc aggtaatatt
     6841 gatagacagc gcgcacattg tgccggaccg tcccgtagta gccgcgaacc gccaggtgct
     6901 cgttcaggct gcgcggcagt tccagcgttt ccgcaatctc aggagcggtc aggccggcat
     6961 tcatcagccg gaccgtctgg tcatggatgt agcggtagat gtcgcgctgc ttgacgatga
     7021 aatcgcggat gcgctccctg ccccagaccg gccagtgatg ctggttgaag acgacgtcgg
     7081 cattcgccgt ttgggataac gcctggtcga tgtaatccgc ccatttcagc gcatctcgca
     7141 ccttggtgcc gcgcagcgtg tagagattgt gctgtgtgaa gctcaacagg tcggcaccac
     7201 agaatgcctt aaggctgggg atctggaaga cgaactccga cggtgcctcg ctttccggta
     7261 cattgtggaa gatgaaccgg actccatcga cgtcaagcgc ctcttgccgt tgctcgatga
     7321 tccgggtcgg cggcaggatg ccgatactgc catacgccac ggccttgccg aggccgttgt
     7381 caatcaggcc ctgcggattg cgcggcaggc ggctgccgta catgtaggtg gagcgccgcc
     7441 ccatggcgac gcccatcaac acgttctcgc tggtggcctc ctccaggaac ccggccggcg
     7501 caatgatcgg aatctgccgc gccttgacct cttcggcgga aaccagaccc agcgtgccgc
     7561 cgaaatgatc ggcatggctg tgggtgtaga tgatggcgga gatcggcttg ttccccagat
     7621 gctggcgcgc gaaggcaatg gccgtggcgg cggtctcgcg tgccgtcagc gtatccacca
     7681 cgatccagcc cgtgcggccc tcgatcagcg tcatgttggc gagatcgaac ccgcgcagct
     7741 gccagatccc ctccgtgacc ttgaaaagtc cgatctcgtt gttgagcctt gcctgccgcc
     7801 agaggctcgg gttgaccgtt ggcggcgcag caccgttcac gaaagcaaag gagtcgaaat
     7861 cccagatcgt ttcgccgctg gcgctctgga ccttgcccga gggccgcgca atgaacccgc
     7921 gccttgcatc cgcgaacgtc gactcgtccg cgaggttcag gcccgccgtc gaggcctgat
     7981 gcgcctgccg cacggcatcc gaggcactgg acgccgtgaa ggcccgtttg ggaggcgccg
     8041 cggggcgcca gagcgccgcg cctgccgcaa cgccggcggc gacacccgtc aggacgccgc
     8101 caa
//
LOCUS       DAOMXN010000107         8091 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_4648_length_8091_cov_10.764435, whole genome shotgun sequence.
ACCESSION   DAOMXN010000107 DAOMXN010000000
VERSION     DAOMXN010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 8091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8091
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4648_length_8091_cov_10.764435"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..178)
                     /locus_tag="PK812_08540"
     CDS             complement(<1..178)
                     /locus_tag="PK812_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012322273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HRJ69642.1"
                     /translation="MTASPSGGFDDPKPADSVSFALIDRVLPLRWRPYARLARLDRPI
                     GWWLLLLPCWWSSAL"
     gene            complement(175..615)
                     /locus_tag="PK812_08545"
     CDS             complement(175..615)
                     /locus_tag="PK812_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69643.1"
                     /translation="MGTVRKAVWVAAFMGLSGAEPAWAQMPQSCMNDFKPLMDARQTA
                     VQRINGLNPKKTTATQACSAFRDLAARNKKVVEYMTANKDWCQIGDADLAAVTQAQDQ
                     IEKNRANTCNAAAKQAAQVRQMQRQQQSAQPGVGSGVRLPQGAL"
     gene            726..1472
                     /locus_tag="PK812_08550"
     CDS             726..1472
                     /locus_tag="PK812_08550"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HRJ69644.1"
                     /translation="MPRYDFSSHRLFVTHPLTPGASIALTREQANYLVSVLRLQEGDG
                     LLIFNGSDGEWRARLHGGTRKAPLLECLGQVRPQPPAGDLWLVFAPLKSARLDYMVQK
                     AVEMGASRLVPVITRRTQVARVNLARMQANAMEAAEQCGILAVPEVAAESRLDQVLAQ
                     WPAGRTLVFCDEDAESDDPLAALAGLGRDAPKALLIGPEGGFDPTERDLLSRVPGCVR
                     LSLGPRILRADTAAVAALALVQAVAGDMCA"
     gene            1552..2922
                     /locus_tag="PK812_08555"
     CDS             1552..2922
                     /locus_tag="PK812_08555"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HRJ69645.1"
                     /translation="MARDTVDMVPISGRADLVGWLEQGIKTQDAFRIGTEHEKFPFYL
                     ETRRPVPYEGATGIRAILEGLQAKTGWSPILEDGNPIGLFDEKGGGAISLEPGGQFEL
                     SGAPLDTLHQTEAELALHLDQVNAVAEPLGIGFLGLGMSPLWTRGQTPVMPKSRYKLM
                     TAYMPTVGSLGIDMMYRTCTVQVNLDFSSEADMVKKLRVSLALQPLATALFANSPFTE
                     GRPNGYKSFRSHVWTDTDGARSGMLPFAFEDGFGFERYVDYALDVPMYFVKRGDHYHD
                     VLGASFRDLLAGRLKQVPGATATVSDWANHLSTIFPEVRLKRYLEMRGADAGSAPHML
                     AQSAFWVGLLYDQRALDASWDIVRSWTADQRQALRESVPRLGLQAVIGGRSLQSIGQE
                     VLALSRGGLAARNRVDDRGRDESRHLDILEARVQSGRVAADDWLERFRGSWGESIRPV
                     FSEARL"
     gene            2949..3476
                     /locus_tag="PK812_08560"
     CDS             2949..3476
                     /locus_tag="PK812_08560"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019903005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HRJ69646.1"
                     /translation="MLAGELYSAADAELQGELAATQRWLKRYNDSLDQPVEARQTLLR
                     QRFGSVGTGCDVRPPFHCDYGFNIHLGDGVFLNFNCVILDIVAVHVGERTQIGPGVQI
                     LTADHPRDAAQRAAGLEFGRPISIGSNVWIGAGALILPGITIGDDAIIGAGSVVTRDV
                     PAGTRAVGNPARQVP"
     gene            complement(3478..4332)
                     /locus_tag="PK812_08565"
     CDS             complement(3478..4332)
                     /locus_tag="PK812_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HRJ69647.1"
                     /translation="MSFDPRLTPARPDLAAARLRGKVEAERFVDGVARRLIVPATPLK
                     REPRPDAPYETEALAGEPVTVYDEQEGWAWVQLETDGYVGWLAAQALGPAEPAPTHRV
                     AALRTFLYPGPSIKMEPLGFLSLGARVTVLRLDGAFAATTIGFVHAGHLAGLDEVERD
                     YVSVAARFVGAPYLWGGKSSLGLDCSGLVQLSLDAAGVRAPRDSDMQAARLGAVLPLD
                     DPQLRRGDLVFWKGHVGIMEDAGTLLHANGHHMAVAREPLAEAIARIQQKSHGPVTAY
                     RRPTGGLG"
     gene            complement(4329..4682)
                     /locus_tag="PK812_08570"
     CDS             complement(4329..4682)
                     /locus_tag="PK812_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HRJ69648.1"
                     /translation="MAVNFRPVQALRLWHEVMVDLVRDEEVDLSARQLAILLTIYLEV
                     PPHTVRGLAKRLNVTKPVITRALDTMGSLDLVSRRRDDLDRRNVIVQRTVKGTLFLDR
                     LSDRIMARIEMLKTA"
     gene            complement(4748..6133)
                     /locus_tag="PK812_08575"
     CDS             complement(4748..6133)
                     /locus_tag="PK812_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HRJ69649.1"
                     /translation="MPEPILIARRPAASRPIHFAHAGNWAEVAATLSDCARRYLEATG
                     FKASAGKIAIAPGADGEIEAVLLGIEGPESPTRDLFAAGRLPGALPAGTYHFASAPER
                     SDLAALAFAMACYRFAAYKKNEPPKVQLVTPKGVDGGRIERIAAAVAMGRDLINTPAQ
                     DMQPTALAAAAKALAQKHEVKAKEIVGDTLLKQNFPMIHAVGRAAAEAPRLIDFSWGN
                     PKHPKVTLVGKGVCFDTGGLNIKPDAAMLLMKKDMGGAATALTAARLVMDAKLAVRLR
                     VLLPIVENAVSAASFRPGDILKSRKGLTVEIGNTDAEGRLVLADALTLADEDKPDLVL
                     DFATLTGAARVALGPDLPPFYSDDNALATEIEAAGQAVNDPVWRLPLWRGYDSMLDSK
                     IADMNNTATGGFAGSITAALFLRRFIDARSWAHFDVYAWSPSAKSGLPEGGNPQVARL
                     VLELLERRYGR"
     gene            6245..7066
                     /locus_tag="PK812_08580"
     CDS             6245..7066
                     /locus_tag="PK812_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ69650.1"
                     /translation="MRAVSNPGFVAVRPWLRAGMLALALAGLAGCKGKGIDFSARLPE
                     PATLDHGQLKAQADALGKQYDKKPGEKQVSLRYAQVLRQLSLHGQAAAVLERTAMTNS
                     NDPEVAGAYGKILADVGRLKEAAEVLARAHTPDRPDWKVLSAQGAVADQLGHHARAQE
                     FYQNALQVSPGEPAVLANLGLSYALTRRLPEAEDALRKAAEHPRADKRVRANLALVLA
                     LQGKFKESEDMARRDLAPREAESNVQEIRKLISQTNSWQEIQKSELAKAQKQAGR"
     gene            complement(7114..7500)
                     /locus_tag="PK812_08585"
     CDS             complement(7114..7500)
                     /locus_tag="PK812_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HRJ69651.1"
                     /translation="MSLQPLLAATPAIQVHFVAAAAALLSGAAVLFLTKGTRLHRLIG
                     GLAAAALLVTAISSFWIRHWGGYSPIHLLSILTLVSVSLGIWFRRRGDLANHKRWMLG
                     AYFGLIGAGLFTILPGRLMHAVVFGD"
     gene            complement(7497..7997)
                     /locus_tag="PK812_08590"
     CDS             complement(7497..7997)
                     /locus_tag="PK812_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008528736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2852 domain-containing protein"
                     /protein_id="HRJ69652.1"
                     /translation="MSCASSWNRKARTWSATDGGGASSPFWPGGAGDRAHESFPGRWW
                     VLGKMGLVAMILGFIVFWPIGLAILFYNMFARKGGELPFTMPPGSHFWSRPTTGNSAF
                     EEWRKAEIERIEQERKKLHEAEREFSVFLDELKRVKDREEFERFMKARKGWTGPEGEP
                     KAEPAA"
BASE COUNT         1439 a         2572 c         2636 g         1444 t
ORIGIN      
        1 ccagggcgct cgaccaccag caggggagca gcagcagcca ccagccgatg gggcggtcca
       61 gccgcgccag gcgcgcgtac ggccgccagc gcagcggcag aacacgatcg atcagggcga
      121 aggagacgga gtcggcgggc tttggatcat caaacccgcc agacggcgat gcggtcacaa
      181 cgcgccctgc ggcaagcgca cgcccgaacc gactccgggc tgggcgctct gctgctgccg
      241 ttgcatctgc cgcacttgcg cggcctgctt ggcggcggca ttgcaggtgt tggctcggtt
      301 cttctcgatc tggtcctgcg cctgcgtcac tgccgcgaga tcggcgtcgc cgatctggca
      361 ccagtccttg ttggcggtca tgtattcaac gaccttcttg ttcctggcag caagatcacg
      421 gaacgcggag caggcttgcg ttgcggttgt ttttttcggg ttgaggccat tgatgcgctg
      481 cacagcggtc tgacgtgcat ccatgagcgg cttgaagtca ttcatgcagc tctgcggcat
      541 ttgcgcccac gccggctccg cgccagacaa tcccatgaat gcggccaccc agactgcctt
      601 gcgcaccgtg cccataccgg atctccgtaa cctcaacgcc tgatcgccca ttgtaagcct
      661 ttctcttcaa tcgcaaacac aggcagaatc aaggcaaaag attccctgtc gtcggagttt
      721 tgggcatgcc gcgctatgat ttttcctcgc atcgcctgtt tgtgacgcat ccgttgacac
      781 caggcgcctc catcgcactg acgcgcgaac aggccaatta tctcgtctcc gtcctgcgcc
      841 tgcaggaggg agacgggctc ctgattttca acggcagcga tggagaatgg cgggcgcgcc
      901 ttcatggggg aacgcgcaag gcgccgttgc tggaatgcct ggggcaggtc cggccacaac
      961 ctcccgcagg ggatctctgg ctggtcttcg cgccactgaa gagcgcccgt ctcgactaca
     1021 tggtgcaaaa agcggtggag atgggcgcct cgcggttggt gcccgtgatc acgcgtcgca
     1081 cccaggttgc ccgcgtcaat cttgcccgca tgcaggccaa cgcaatggag gcggccgagc
     1141 aatgcggaat acttgcggtt ccggaggtcg cagccgagtc tcgcctggac caggtcctgg
     1201 cgcagtggcc cgccggtcgg acgctggttt tttgcgatga ggacgctgaa tctgacgatc
     1261 cgctggcagc gctcgctgga cttggtcggg acgcgcccaa agcccttctg attggccccg
     1321 agggcgggtt tgaccctaca gagcgggatc tgctctcccg ggtgccggga tgtgtgcgcc
     1381 tgtcgctcgg gccgcgcatt cttcgggcgg acaccgccgc tgttgcagcc ctggcgctgg
     1441 tccaggcggt cgcgggcgac atgtgcgcct gacggttgca gcatccgctt cccgccatta
     1501 tatcgggggg caacctattg gcgcctggcg ccgcatccgg gatcaccgtt catggctcgc
     1561 gataccgtcg atatggtccc catctcaggc cgcgccgatc tggtcggctg gctggagcag
     1621 ggcatcaaga cgcaggatgc ctttcgcatc ggcacggaac acgaaaagtt tcccttctac
     1681 ctggagaccc gacgccctgt tccttacgag ggcgcgaccg gtatccgcgc gatcctcgaa
     1741 ggacttcagg cgaagacggg ctggtccccg attttggagg acggcaatcc catcgggctg
     1801 ttcgacgaaa agggcggtgg cgcgatctcg ctggagcccg gcggacagtt cgaactctcc
     1861 ggcgcaccgc tggacacact gcaccagacc gaagcggaac tcgctctcca cctcgatcag
     1921 gtcaatgcgg tggcggaacc cctcggaatc ggcttccttg gattgggcat gagcccgctc
     1981 tggacgcgtg gccagacacc ggtaatgccg aaatcccgct acaagctcat gaccgcctac
     2041 atgccgacag tcggtagcct gggcatcgac atgatgtaca ggacctgcac ggtccaggtg
     2101 aatctcgact tctcgtcaga agccgacatg gtcaagaaat tgcgcgtctc gctggcgctg
     2161 cagccgctgg cgacggccct gttcgccaat tctcctttca ccgaggggcg tccaaacgga
     2221 tacaagtcgt tccgctccca tgtctggacg gacacggacg gcgcgcggtc gggcatgctc
     2281 ccgttcgcat tcgaagatgg cttcggattc gaacgatatg tcgactatgc acttgatgtg
     2341 ccgatgtatt tcgtcaaacg gggggaccat taccacgacg ttctgggcgc ttcgttccgt
     2401 gatctcctgg cgggcaggct caagcaggtg ccgggcgcga ccgcgacggt ctccgactgg
     2461 gccaaccacc tgtcgacgat ttttccggaa gttcgtctga agcggtatct tgagatgcgc
     2521 ggagcggacg caggatcggc tccccacatg ctggctcagt ccgccttctg ggtcgggttg
     2581 ctctacgatc agcgcgccct cgacgcgtcc tgggacatcg tgaggtcctg gacggcggat
     2641 caacggcagg ccctgcgcga aagcgtgccc aggctggggc ttcaggccgt gatcggcggc
     2701 cgaagcctgc aaagtatcgg gcaggaggtc ctggcgctgt cgcgcggcgg tcttgccgcc
     2761 cgcaatcggg tcgatgaccg tgggcgcgac gagagccggc atctcgatat tctcgaagcg
     2821 cgcgtgcagt cggggcgcgt ggccgccgac gactggcttg agaggtttcg cggatcgtgg
     2881 ggagagagca tccgcccggt gttctccgag gcgcgactgt gagccaagcg ccgagccaga
     2941 agcaacggat gctggcaggc gaactctact ccgccgccga cgcggagctg caaggcgagc
     3001 tggccgcaac ccagcgctgg ctcaagcgat acaatgacag cctcgaccag ccggtggagg
     3061 cacgccagac actgctgagg cagcgctttg gcagcgtcgg aacaggatgc gatgtccgcc
     3121 cgccgttcca ttgcgactat ggcttcaaca tccatcttgg cgacggtgtt ttcctcaatt
     3181 tcaactgcgt gatccttgac atcgtcgcgg ttcatgtcgg cgagcgcacc cagatcggac
     3241 cgggcgtgca gattctcacg gccgatcatc cgcgcgatgc cgcacagcgg gccgcgggtc
     3301 tcgagtttgg ccggccaatc agtatcggca gcaatgtctg gatcggagca ggcgcgttga
     3361 tcctgccggg catcaccatc ggtgacgacg cgatcatcgg cgccggcagc gtcgtcaccc
     3421 gcgatgtgcc ggccggaacg cgcgcggtcg gcaatccggc gcggcaggtg ccataggcta
     3481 tcccagaccg cccgttggcc ggcgataggc ggtaaccggc ccgtgcgact tctgctgaat
     3541 gcgtgcgatg gcttccgcca gcggttcgcg cgctacggcc atgtgatgac cgttggcgtg
     3601 aagcaatgtc ccggcatctt ccatgatgcc gacgtggcct ttccagaaca cgagatcgcc
     3661 acgccgcaat tgcggatcat caagcggcag caccgcgcca agccgcgccg cctgcatgtc
     3721 ggaatcgcgc ggggcacgca ctccggccgc atcgagtgac agttgcacca gaccggaaca
     3781 gtcgaggccg aggctcgact taccgcccca aagatacgga gcgccaacga agcgcgcggc
     3841 cacgctcacg tagtcgcgct ccacttcgtc caggccggca agatgaccgg cgtgcacgaa
     3901 gccgatggtc gtggcggcga aagcgccgtc cagcctgagt acagttaccc gagcgcccag
     3961 cgacaggaag ccgagaggct ccatcttgat gctgggcccg ggatagagaa acgtgcgcag
     4021 cgccgcgacc cgatgcgtcg gcgccggttc cgccgggccg agtgcctgcg cggcaagcca
     4081 cccgacatag ccgtcggtct ccagctggac ccaggcccac ccctcctgct cgtcgtagac
     4141 cgtgaccggc tctcccgcga gcgcttcggt ctcgtaggga gcatccgggc gcggttcccg
     4201 cttgagcggc gttgccggca cgatgaggcg tcttgcaacc ccatcgacga atcgttccgc
     4261 ttcgaccttt ccgcgaagtc tcgcagcggc aaggtccgga cgagccggtg tcaggcgggg
     4321 atcgaagctc atgcggtctt caacatctcg atgcgcgcca tgatccggtc ggacagccga
     4381 tcgaggaaaa gcgttccttt gacggtgcgc tgcacgatca cgtttcgccg gtcgagatcg
     4441 tcccggcggc gtgaaacaag gtcgagactg cccatcgtgt ccagcgcccg cgtgatcacc
     4501 ggcttggtca cgttgagccg ctttgccagc ccacgcacgg tatggggtgg cacttccaga
     4561 taaatcgtca gcaggatcgc gagctggcgc gccgagagat cgacctcctc gtcccgaacg
     4621 aggtcgacca tcacctcatg ccagagcctg agcgcctgaa ccgggcggaa gttcaccgcc
     4681 atggtcgctc cttcatttcg gagccgtatt attcttcatc ccgcgagcaa atgcaaaggg
     4741 cgccaatcta gcggccatag cgccgttcca gaagttccag caccagcctg gcgacctgcg
     4801 ggttgccgcc ctccggcaga ccggacttgg cggatggcga ccaggcatag acgtcgaaat
     4861 gggcccagga gcgcgcatcg atgaagcggc gcaggaaaag cgccgctgtt atggagccgg
     4921 caaagccccc tgtggcggta ttgttcatgt cggcgatctt ggagtccaac atcgaatcgt
     4981 agccgcgcca caggggcagc cgccagaccg gatcgttcac cgcctggccg gctgcttcga
     5041 tctcagtcgc gagagcattg tcgtccgaat agaagggggg cagatccggg cccagcgcga
     5101 cgcgcgccgc tccggtcagc gtggcgaagt ccaggacgag atcgggttta tcctcgtcgg
     5161 cgagtgtgag cgcatccgca agaaccagcc gtccttccgc gtcggtgttg ccgatttcca
     5221 cggtcaggcc cttgcggctc ttcaggatgt cgccgggtct gaacgaagcg gctgaaaccg
     5281 cgttctccac gatcggcagc aggacgcgca ggcgcacggc aagcttggca tccatcacga
     5341 ggcgtgcagc ggttagcgcc gtagccgcgc cccccatgtc cttcttcatc aggagcatcg
     5401 ccgcgtccgg cttgatgttg aggccacccg tgtcgaaaca gacacctttg ccgaccagcg
     5461 tcacctttgg gtgtttcggg tttccccaac tgaagtcgat caggcgggga gcctcggcgg
     5521 ccgcgcggcc caccgcgtgg atcatcggga agttctgctt gagaagcgta tcgccgacga
     5581 tctccttcgc cttgacctcg tgcttttgcg caagagcctt tgccgcggcg gcgagggcgg
     5641 tcggctgcat gtcctgagcg ggcgtattga tgaggtcgcg acccatcgcg acggccgccg
     5701 cgatgcgctc gattctgcct ccgtcgacgc ctttaggcgt cacgagttgg acctttggcg
     5761 gttcgttctt cttgtaggcg gcaaagcggt agcacgccat ggcgaaggcc agagccgcga
     5821 gatccgaccg ctccggggcg ctggcgaagt gataggtccc ggcgggcagc gctccaggga
     5881 gtcggcccgc tgcaaacagg tcgcgggtgg gtgattccgg tccttctatg ccgaggagga
     5941 cggcttcgat ctcgccgtcc gcgccgggcg caatcgcgat ttttcctgcg ctggccttga
     6001 aacccgttgc ttccaggtag cggcgtgcgc agtcggaaag cgtggctgcg acctcagccc
     6061 agtttccggc atgggcgaaa tggatcgggc ggcttgcagc cgggcgtcgg gcgatcagga
     6121 tcggttcggg catgacgggg tccgtggtca gatgaggcgc cgcattaacc aacgattagc
     6181 gttaacgaac tattgctggc gaggcgagcc ggaaggaccg gtcgcagcga agaggtctcg
     6241 cgccatgcgt gctgtatcga atccgggttt cgtcgctgtg cggccgtggc tgcgtgccgg
     6301 aatgctggcg ctggccttgg ccggactggc aggctgcaag ggcaagggca tcgatttttc
     6361 cgcaagactg cccgagccgg ccacgctcga tcacggccag ttgaaggcgc aggccgatgc
     6421 gctcggcaag cagtacgaca agaagccggg agagaagcag gtctcgttgc gctatgcgca
     6481 ggttctgcgc cagctcagtc tgcatggtca ggccgccgcg gttcttgaac gtaccgcgat
     6541 gacgaattcg aacgacccgg aagttgcagg cgcctatgga aagattctgg ccgatgtcgg
     6601 tcggctcaag gaggcagcgg aagtgctcgc tcgggcgcat acgcctgacc gaccggactg
     6661 gaaggtgctt tcggcgcagg gcgcggttgc tgatcagctt ggccaccatg cccgtgccca
     6721 ggaattctac cagaacgccc tgcaggtctc tcccggcgaa ccggcggtgc tggcgaacct
     6781 gggcctttcc tatgcgctga cgcgccgttt gccggaggcc gaagacgcgt tgcgcaaggc
     6841 cgctgagcac ccgcgcgccg acaagcgggt tcgcgccaac ctggcgctcg tcctggcgct
     6901 gcagggcaaa ttcaaggaat cggaagacat ggcgcgtcgg gacctggctc cgcgcgaagc
     6961 cgagtcgaat gttcaggaga tccgcaaact gatctcccag acaaattcct ggcaggaaat
     7021 tcagaagtcg gaactggcca aagcccaaaa gcaggcgggc cgctgatccg gtcgcatgca
     7081 ataccggctt cgtcccgggc gatccgtccg gtgtcagtcg ccgaagacca cggcgtgcat
     7141 cagccggccc ggcagaattg tgaacaggcc ggcgccgatc aggccgaagt aggctccgag
     7201 catccagcgt ttgtggttcg ccaaatcgcc gcgccgtcga aaccagatcc cgagcgacac
     7261 agataccagc gtcaggatcg acaacaggtg gatgggtgag tagccgcccc agtgcctgat
     7321 ccagaacgag gagatggcgg tcaccaggag cgcggcggcc gccagaccgc cgatcaggcg
     7381 atgcaatcgg gtccccttgg tcagaaacag caccgccgcg cccgagagga gcgcggcggc
     7441 tgcggcaacg aaatgaacct ggatcgccgg ggtcgcggcg aggagcggct gcaggctcac
     7501 gcggccggct ctgccttcgg ctcgccttca ggtcccgtcc agcccttgcg ggccttcatg
     7561 aaacgctcga attcctcgcg gtccttgacg cgtttcagtt catcgaggaa caccgaaaac
     7621 tcgcgttcgg cctcgtgcaa tttcttgcgc tcttgctcga tgcgctcgat ctcggccttc
     7681 cgccattcct cgaatgccga gttgcctgtg gtcggccggg accagaagtg cgagcccggc
     7741 ggcattgtga agggcaactc gccgcccttc cgggcgaaca tgttgtagaa cagtattgcc
     7801 agtccgatcg gccagaacac gatgaagcca aggatcatgg cgaccaggcc catcttcccc
     7861 aggacccacc agcggccggg aaaggactcg tgggcccgat cacccgctcc gccaggccag
     7921 aacggactgg aggcgccgcc gccgtccgtc gcggaccagg tgcgcgcctt gcgattccag
     7981 gaagaggcac agctcatgga aagctcccga ggtgaatgtt acaaacataa acataggagc
     8041 gatcaaaacg gattgcaagt gggttcatgc aatattcacg tggtctgcgg g
//
LOCUS       DAOMXN010000108         8079 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_4656_length_8079_cov_8.803340, whole genome shotgun sequence.
ACCESSION   DAOMXN010000108 DAOMXN010000000
VERSION     DAOMXN010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 8079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8079
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4656_length_8079_cov_8.803340"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /locus_tag="PK812_08595"
     CDS             complement(<1..1009)
                     /locus_tag="PK812_08595"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69653.1"
                     /translation="MRAASLFLLLTLAACAGGPDGEQEWLCRRIAPALFDGEKKLTVL
                     SSTAGRSRDVFVRLSVEASPGTHVVYCRFAGSGFSPRKRELVAVSVDGQAMSEAGVHF
                     LREGWLQAQESITAAPPRARPEGEATLGAGTAYALQQALSALPKTGIIVLLCAAYALL
                     YGLTGRINLAFGGFVALGGMAMTIGAAAMDIAGAPGILAPLAFGLACAVTLSALHGLV
                     TAGLLFVPLAARPGQHILIASVGLMIALEEFLRLAQGAGTRWLAPVLNLPVTVARAPT
                     YDVTVTPVALATAGTAAVAAIALLVYLSMSGFGRRWRAVADDPDAAILFGVAPGATIA
                     AS"
     gene            complement(996..2012)
                     /gene="hemB"
                     /locus_tag="PK812_08600"
     CDS             complement(996..2012)
                     /gene="hemB"
                     /locus_tag="PK812_08600"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HRJ69654.1"
                     /translation="MNTLQRIRAATLDIARRPRRNRKADWARRLVAESTLTADDLIWP
                     IFIIDGERKRLPVASMPGVERMTVDLAVREAERAEALGIPAIALFPYTDPARRDETGS
                     EALNPQNLVCTACRAIRAARLNIGVITDVALDPYTSHGHDGLIRDGEILNDETVEVLC
                     RQALTQAAAGADIIAPSDMMDGRVGAIRAALDQSGFQKVSIMAYTAKYASAFYGPFRD
                     AVGANKTLTGDKKTYQMDPANGAEALIEAELDIAEGADMIMVKPGMPYLDVLYRLKTG
                     FGLPTFAYQVSGEYAMIQAAAANGWIDGEKAMLESLVAFKRAGADGILTYFAPQVAEI
                     LARR"
     gene            2158..3204
                     /locus_tag="PK812_08605"
     CDS             2158..3204
                     /locus_tag="PK812_08605"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRJ69655.1"
                     /translation="MSEESEVLVRRQGAAGIIVLNRPKALNALTQTMVREMDRALKAW
                     ERDPAVTRIVVKGAGERAFCAGGDIRALHDLGKAGRQAEALAFWREEYILNRYIKRYP
                     KPYVALIDGIVMGGGVGVSLHGSHRVAGDRIGFAMPEVGIGFFPDVGATYALPRLPFR
                     AGSYLALTGQRIGRADALALGIATHGVASVDFPAVEAGLIAGDDIDTLLKAHESVIEP
                     SPLVAHKDIIERAFGGSSVRAILTALDAEALASEFARAAAATMRTKSPLSMAIALEQM
                     RRGSALTFEEAMTMEMRIVTRVAAGHEFYEGVRALIIDKDQKPAWRPASVDGVDPVEV
                     ATYFAPLPDDLIFA"
     gene            3218..3682
                     /locus_tag="PK812_08610"
     CDS             3218..3682
                     /locus_tag="PK812_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69656.1"
                     /translation="MSDAQTPERADTAPLHTGQWADAPIGAAKEPTGVRWLTILLAFM
                     RIAACFWLLKGILQWAAIIGLSDIDFPELRLSRQGIIIAFAVMDLVAAVGLWLTSSWG
                     AAVWLVLLFAQGAAPFLMPDLTPSVVDALVSAGFGGTYLLLVWKALQEERAQ"
     gene            3961..4473
                     /locus_tag="PK812_08615"
     CDS             3961..4473
                     /locus_tag="PK812_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HRJ69657.1"
                     /translation="MNNVAAAKAMPEAAPQSNPVRGQYLESLALVERLHRRLLDVIKD
                     ELDRRGRTDVNSVQALLLYNIGGNEVTAGELRTRGYYLGSNVSYNVKKLVEMGYLNHQ
                     RSRVDRRSVRIRLTKTGEEVHGMVAHLYEKHCKTVEQIGGISADDFQKLNNALTRLDR
                     FWTDQIRYRL"
     gene            4905..6170
                     /locus_tag="PK812_08620"
     CDS             4905..6170
                     /locus_tag="PK812_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003608366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRJ69658.1"
                     /translation="MNRRLFLMGAVSAGAVSTSAAVAAPNDGGIIGGQAEWRQQYDSS
                     PLRHRPVKSSAPILSPQTIAATEQAVGLLQQVVQRGGWQPLPTSIRLKLGVKHPNVVA
                     LRGRLITGGDLDGTNANSQIFDSYVEAGVKRFQERHGLGATGVVGPQTFAAFNVPSDV
                     RLRQLEINLVRLKTFGQNLNGRYVMANIPAAYVETVENNIVATRHVAGVGKIDRQSPI
                     MSARVLDINFNPFWTVPASIIKKDLIPRMQKDPNYLTEQKIRIYNKAGQELPPQQVNW
                     NSNEATNYMFRQDPGGEFNSLGFVRINIANPHGVYMHDTPAKGIFGDDFRFVSSGCIR
                     VQNVRDFVAWILKDTPGWDRDQIEAAIRSGQRQDARPPAPIPVYWVYVTAWATPEGAM
                     QFRDDIYGRDGFGPGAVTAAIQAGDSEPE"
     gene            complement(6249..7058)
                     /locus_tag="PK812_08625"
     CDS             complement(6249..7058)
                     /locus_tag="PK812_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69659.1"
                     /translation="MWLVDPFDSSGTEVAGREDARRFLLQRTDGVRRQSPDDPEQAMA
                     LAQDVGRLIVPLDHAACLIAHAGMSFGAYRAAWAEASERVRDRFQPGVSLHTRMMTPT
                     WLVTYEALDAEARTLLAVLSFFSPAPVPCAVFVALGESLAAAYRARLDTLAAVGFVRI
                     APDGADFRVQGLVQRLTRHTIGAEQVRANLTAALVWMDFAFAGAVDSPDLRSRFEPLV
                     PHVEQIVAAGESGAIASPTATLMRALGRFHAALGGQNVAASWLARAQALEG"
     gene            7180..>8079
                     /locus_tag="PK812_08630"
     CDS             7180..>8079
                     /locus_tag="PK812_08630"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012253505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HRJ69660.1"
                     /translation="MVEALRIGIAGLGTVGIGALKILTTRKTELALAAGRPIEVVAVC
                     ARDRGRDRGVDLSHVAWFDDPVALAASPAIDVFVELMGGSGDPAKTAVKTALKSRKPV
                     VTANKALLAKEGARLARLAEKKGVALGFEPAAAGGIPVIKALRESLAANRVQRVSGIM
                     NGTCNYILSRMEAEGLTFAECLKDAQRLGYAEADPTFDVEGFDTAHKLALLTSLAFGT
                     EVDSDSIYVEGISNVTPLDLRMADELGFRIKLLGVAERTAQGVEQRVHPTMVPKTSQL
                     AQVMGVLNAVSVVADPVGELTFVG"
BASE COUNT         1374 a         2678 c         2683 g         1344 t
ORIGIN      
        1 aagacgcggc aatcgttgcg ccgggcgcga cgccgaacag aatcgccgcg tccggatcgt
       61 cggcgaccgc ccgccagcgc cggccgaacc cgctcatgct gagatagacg agcagggcaa
      121 tggcggcgac ggccgcggtt ccggccgtcg ccagcgccac gggcgtcacc gtgacatcgt
      181 acgtcggcgc gcgcgcgacc gtgacgggca ggttcagcac gggagcgagc cagcgcgtgc
      241 cggcaccctg cgccagccgc aggaactcct cgagtgcgat catcagcccg acgcttgcga
      301 tcaggatgtg ctgtcccggc cgggccgcca gcggaacgaa cagcaggccc gccgtcacca
      361 ggccatgcag cgccgacagg gtgacggcgc aggccaggcc gaacgcaagc ggcgccagaa
      421 tgcccggcgc tccggcgatg tccatcgccg ccgcgccgat ggtcatggcc atgccgccca
      481 gcgccacgaa gccgccgaaa gcgagattga tccgtccggt cagcccgtag agcagggcat
      541 aggccgcgca gagcagcacg atgatcccgg tcttgggcag ggcggagagc gcctgctgca
      601 aggcataggc cgtgccggcg ccaagcgtcg cctcgccctc gggccgtgcc cgcggcgggg
      661 cggcggtgat cgactcctgg gcttgcagcc agccctcgcg gaggaaatgc acgccggcct
      721 cgctcatggc ctgtccgtcg accgagaccg cgaccagctc gcgctttcgc ggcgagaacc
      781 ccgagccggc gaagcggcaa tagacgacat gcgtgcccgg gctggcctcg accgagagcc
      841 gcacgaagac gtcccgggaa cggccggccg tgctggaaag gaccgtgagc ttcttctcgc
      901 cgtcgaacag ggcaggggcg atgcgtcggc agagccattc ctgctcgccg tccggcccac
      961 ccgcgcaggc cgccagcgtc agcagcagaa aaaggctagc ggcgcgcaag tatctccgcc
     1021 acctgcggcg cgaaataggt caggatgcca tcggcgccgg cgcgcttgaa ggcgaccagg
     1081 ctctccagca tcgccttttc gccgtcgatc cagccgttgg ccgccgccgc ctggatcatc
     1141 gcatattcgc ccgagacctg ataggcgaag gtcggcaggc caaagccggt cttcagccgg
     1201 tagagcacgt cgagataggg catgcccggc ttgaccatga tcatgtcggc gccttcggca
     1261 atgtcgagtt cggcctcgat cagtgcctcg gcgccattgg ccgggtccat ctggtaggtc
     1321 ttcttgtcgc cggtcagcgt cttgttggcg cccaccgcgt cgcggaaagg cccgtagaag
     1381 gccgaggcat acttggccgt gtaggccatg atcgagacct tctgaaaccc ggactggtcc
     1441 agggcggcgc ggatcgcgcc gacgcggccg tccatcatgt cggacggggc aatgatatcg
     1501 gcgccggcgg cggcctgagt cagcgcctgc cggcacaaca cctcgacggt ctcgtcattc
     1561 aggatctccc catcgcggat caggccgtcg tggccgtgcg aggtgtaggg atcgagggcc
     1621 acgtcggtga tgacgccgat gttcagccgc gccgcccgga tcgcccggca ggcggtgcag
     1681 accaggttct gcgggttcag cgcttccgat cccgtctcgt cgcgcctggc cgggtccgtg
     1741 taggggaaca gcgcgatcgc cggaatgccg agcgcttcag cgcgctccgc ctcccgcacg
     1801 gcaagatcca cagtcatccg ctcgacgccc ggcatggagg cgaccggaag ccgctttctt
     1861 tcgccgtcga tgatgaagat cggccagatc agatcatcgg ccgtcagcgt gctctcggcg
     1921 accagccggc gcgcccagtc ggccttgcgg ttgcggcggg gacggcgggc gatgtcgagc
     1981 gtcgccgccc ggatgcgctg cagggtgttc atggcatgtc caacgcgaac gaatgaccgg
     2041 cgcatcatag gcgtcctttg ccggacgcgg aagggcgtgt ttgctccgat acgccgcgcc
     2101 atttgacgcc ccacgcctgc acgcgctacc tgcggagagc gacaggagcc gttcgacgtg
     2161 agtgaagaat cggaagtgct ggtgcgccgg cagggtgcgg ccgggatcat cgtcctcaac
     2221 cggccgaaag cgctgaacgc gctgacgcag accatggttc gggagatgga ccgcgccctc
     2281 aaggcctggg aacgcgaccc tgcggtgacc cggatcgtgg tcaagggcgc gggcgagcgc
     2341 gccttctgcg ccgggggcga cattcgcgcc ctgcacgatc tcggcaaggc cgggcgtcag
     2401 gcggaggcgc tggccttctg gcgcgaggag tacatcctca atcgctacat caagcgctat
     2461 ccgaagccct atgtcgcgct gatcgacggc atcgtcatgg gcgggggcgt cggggtttcg
     2521 ctgcacggtt cgcaccgcgt cgccggcgac cggatcggct tcgccatgcc ggaggtcggc
     2581 atcggcttct tccccgacgt gggcgcgaca tacgccctgc cgcgcctgcc tttccgtgcg
     2641 gggagctatc tggcgctcac cggccagcgt atcgggcgcg cggatgccct ggcgctgggc
     2701 atcgcgaccc acggtgtggc gagcgtcgat ttccctgccg tggaggctgg gctgatcgcc
     2761 ggcgacgaca tcgacacgct gctcaaggcg catgagtcag tcatcgaacc ctcgccgctg
     2821 gtcgcgcaca aggacatcat cgagcgggcc ttcggcggca gttcggtgcg ggcgatcctg
     2881 actgctctcg acgcggaggc gcttgcgtcc gaattcgcgc gcgccgccgc cgccacgatg
     2941 cggacgaaaa gtccgctcag catggccatt gccctggagc agatgcgcag gggctcggcc
     3001 ctgaccttcg aggaggcgat gacgatggaa atgcgcatcg tgacgcgcgt ggccgccggc
     3061 cacgaattct acgaaggcgt gcgtgcgctg atcatcgaca aggatcagaa gccggcatgg
     3121 cgccccgcct cggtggatgg cgtcgacccg gtggaggtcg cgacctattt cgcgccgctt
     3181 cccgatgacc tgatcttcgc atgagcaccc ccggcgcatg agcgacgcac aaacgcccga
     3241 gcgcgccgac accgcgccgc tgcacaccgg ccagtgggcg gatgcgccca tcggcgcggc
     3301 gaaggaaccg accggcgtgc gctggctgac gatcctgctc gccttcatgc gcatcgctgc
     3361 ctgcttctgg ctgctcaagg gcatcctgca atgggcggcg atcatcggcc tgtcggatat
     3421 cgattttccc gaactgcgcc tgagccgtca gggcatcatc atcgccttcg ccgtcatgga
     3481 cctggtcgcg gcggtcggtc tctggctcac gagttcctgg ggtgcggcgg tctggctggt
     3541 gctgctgttc gcgcagggcg ccgcgccctt tctcatgccc gaccttaccc cctcggttgt
     3601 cgatgcgctg gtcagcgccg ggttcggcgg gacctacctg ctgctggtct ggaaggcctt
     3661 gcaggaagag cgcgcccagt agccagcgcc ggcccaagaa agcctgcaag gtacgggcgg
     3721 gtgcgcccgt gcagagtcaa ttccgagtca atttgaatag taaacaagtc tttgtcgttg
     3781 aaattcggca atattaatgc ctccttttcg taaaactcga ttcacattag cgcgtaaatc
     3841 gagcgtttag gctggaattt aaactcgtat tcattcggcg cctgtaaggt ccttgcaact
     3901 gaccgggcaa ttccggcgac acgatcccaa acgggacgcc agtaaacagg gacgagagtc
     3961 atgaacaacg tagcagccgc taaggctatg ccggaagccg cgccgcagtc gaacccggtt
     4021 cgcggacagt atcttgaatc gctggcgctg gtggagcgcc tgcatcgccg cctcctcgat
     4081 gtgatcaagg atgaactcga tcgtcggggc cgcacggatg tcaacagcgt ccaggcgctg
     4141 ctgctctaca acatcggcgg caacgaggtg acggcgggtg aactgcgcac ccgcggctat
     4201 tatctcggtt cgaacgtctc ctacaacgtg aagaagctgg tcgagatggg ctatctcaac
     4261 catcagcgtt cgcgcgttga ccgccgttcg gtgcgcatcc gtctgacgaa gacgggcgag
     4321 gaggtccacg gcatggtggc ccatctctat gaaaagcact gcaagacggt ggaacagatc
     4381 ggcggcattt ccgctgacga cttccagaag ctcaacaacg cactgacccg gcttgaccgc
     4441 ttctggaccg accagatccg ctatcggctc taaggctgcc cgcgaacctt tcggttcacg
     4501 gggatctgga cggaccagat ccgctatcgg ctctaaggct gcccgcgaac ctttcggttc
     4561 acggggatct ggaccgacca gatccgctat cggctctgat cggaaatccg acgacgaacc
     4621 tccaaagact tagagacccc gtgcaagcgg ggtctttttt ctgtcaggct ctgcgtcctc
     4681 gctggccagt gacgcaattg cgcaacgctg cccgctcaaa tccccgtcat cgtcacaaat
     4741 ttgcccggat atgccttgca taccggcggt taacgatatt tcgaccagca gcgggcattc
     4801 tgcctgtcat ggcgcagtgg cgatcggccg gggcgcagac tggtaaagcg tacggagatt
     4861 ggcgagtgac tgggacttgg cgaccgtttg gtggtgattt gggcgtgaat cgccgtcttt
     4921 ttctgatggg cgcggtttcg gccggtgcgg tgtcgacttc ggcggctgtc gccgccccca
     4981 acgacggcgg catcatcgga ggccaggctg aatggcgcca gcagtatgat tcctcacccc
     5041 tccgccacag gccggtgaag tcttccgcgc cgattctctc gccgcagacc atcgccgcga
     5101 ccgaacaggc cgttggcctg ttgcagcagg tggtgcagcg gggcggctgg caaccgttgc
     5161 cgaccagcat acgcctcaag ctcggcgtca agcatccgaa tgtcgtggcg ctgcgtggcc
     5221 gcctgatcac cggcggcgat ctggacggca ccaacgccaa ttcccagatt ttcgactcct
     5281 atgtcgaggc cggcgtgaag cgcttccagg agcgtcacgg actcggcgcg acgggtgtcg
     5341 tcggtccgca gaccttcgcg gccttcaacg ttccctccga tgtgcgcctg cgccagttgg
     5401 agatcaacct cgtccgcctc aagaccttcg gccagaacct caacggccgc tacgtgatgg
     5461 ccaacatccc ggcggcctat gtggagacgg tggagaacaa catcgtcgcc acgcgccacg
     5521 tcgccggcgt cggcaagatc gaccgccagt cgccgatcat gtcggccagg gtgctcgata
     5581 tcaacttcaa tccgttctgg accgtgccgg catcgatcat caagaaggat ctgatcccgc
     5641 gcatgcagaa ggatccgaat tacctgaccg agcagaagat ccgcatctac aacaaggcgg
     5701 ggcaggaact gccgccgcag caggtgaact ggaattccaa cgaagcgacg aactacatgt
     5761 tccgtcagga cccgggcggc gagttcaatt cgctcggctt cgtgcgcatc aacatcgcga
     5821 atccgcatgg cgtctacatg catgacacgc cggcgaaggg cattttcggc gatgacttcc
     5881 gcttcgtgtc ctccggctgc atccgcgtgc agaatgtgcg cgacttcgtg gcctggattc
     5941 tcaaggatac gcccggctgg gatcgcgacc agatcgaggc ggccatccgt tccggccagc
     6001 gccaggatgc ccgcccgccg gctcccatcc ctgtctactg ggtttatgtc accgcctggg
     6061 cgacccctga aggcgcgatg cagttccgtg acgacatcta tggccgcgac gggttcggcc
     6121 cgggcgcggt gacggccgcc atccaggccg gcgacagcga gccggaatag gcccgcgttc
     6181 tcgcgcggat catggcaagg ggcctgcggg cccctttttc atgggtggcc ccgcatttcc
     6241 ggacaggctt agccttcaag ggcctgcgcc cgcgccagcc atgaggccgc gacgttctgt
     6301 ccgcctaggg cggcatggaa gcggccaagg gcgcgcatca gcgtcgccgt cggcgatgcg
     6361 atcgcgccgg attcgcccgc cgcgacgatc tgctcgacat gcgggaccag cggttcgaat
     6421 cgtgaccgca ggtccgggct gtcgaccgcc ccggcgaaag cgaagtccat ccagaccagc
     6481 gcggccgtga ggtttgcccg gacctgctcg gcgccgatgg tgtgacgggt cagtctctgg
     6541 accaggccct gcacccggaa gtcggcgcca tccggcgcga tgcggacgaa cccgaccgcc
     6601 gcgagcgtgt cgagccgggc gcggtaggcg gccgccagac tttcccccag cgcgacgaag
     6661 acggcgcacg gcaccggcgc gggagaaaaa aacgacagca cggccaagag ggtgcgcgcc
     6721 tcggcatcaa gcgcctcgta ggtgaccagc caggtcggcg tcatcatccg ggtatgcagc
     6781 gacacgccgg gctggaaccg gtcccgcacc cgctccgagg cttccgccca ggccgcccga
     6841 taggcgccga agctcatgcc ggcatgggcg atcaggcagg cggcatgatc cagcggcacg
     6901 atcaggcggc cgacgtcctg cgccagcgcc atcgcctgtt ccggatcgtc gggcgactgg
     6961 cgccggacgc cgtcggtgcg ttgcagcagg aagcggcgcg catcttcgcg tcccgccacc
     7021 tccgttccgg aggaatcgaa tggatcgacg agccacatgt cccgctctcc tcgcaggata
     7081 gagactaggc ttcgccgttc gatcgggcaa tctgcattgc ccgtcccgcc caactccgct
     7141 aatccagcgt cttgctgcat tgcacacatg gacagggcta tggttgaggc gcttcggatt
     7201 ggcatcgcgg gcttgggtac ggtcgggatc ggcgcgctga aaatcctgac aacccgcaag
     7261 accgagctgg cgctggccgc cggccggccg atcgaggtcg tcgcggtctg cgcccgcgac
     7321 cggggacgcg atcgcggggt ggacctttcc catgtcgcct ggttcgacga ccccgtggcc
     7381 ctcgccgctt cgccggccat cgacgttttc gtcgagctga tgggtggttc gggggatccc
     7441 gccaagaccg cggtgaagac cgctctcaag agccgcaagc ctgtagtgac cgccaacaag
     7501 gccctgctgg cgaaggaggg cgcgcggctg gcccgccttg ccgagaagaa gggcgtggcg
     7561 ttggggttcg agccggcggc cgccggcggc attccggtga tcaaggcgct gcgcgagtcg
     7621 ctggccgcca accgcgtgca gcgcgtctcc ggcatcatga acggcacctg caactacatt
     7681 ctcagccgca tggaagccga ggggctgacg ttcgccgagt gcctgaagga cgcgcagcgg
     7741 ctgggctatg ccgaggccga cccgaccttc gacgtcgagg gtttcgacac ggcgcacaag
     7801 ctggcgctgc tcacctccct cgccttcggc acggaggtgg attcggactc gatctatgtc
     7861 gagggcatct cgaacgtcac cccgctcgac ctgcgaatgg cagacgaact cggcttccgg
     7921 atcaagctgc tcggtgtcgc cgaacgcacg gcgcagggcg tcgaacagcg cgtgcacccg
     7981 acgatggtgc cgaagacctc gcaactggcg caggtcatgg gcgtcctgaa cgcggtgtcc
     8041 gtggtcgccg atccggtcgg agaactcacg ttcgttggc
//
LOCUS       DAOMXN010000109         8056 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_4681_length_8056_cov_8.700412, whole genome shotgun sequence.
ACCESSION   DAOMXN010000109 DAOMXN010000000
VERSION     DAOMXN010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 8056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8056
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4681_length_8056_cov_8.700412"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /gene="secG"
                     /locus_tag="PK812_08635"
     CDS             complement(<1..272)
                     /gene="secG"
                     /locus_tag="PK812_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017483438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HRJ69661.1"
                     /translation="MDTVLIVIHLIIVVALCGTVLLQRSEGGGLGMGGGSGTPSGFLT
                     GRGQANLLTRTTAILGAAFFATSLILSIMAARSRAPVTVNFVNADI"
     gene            complement(380..1381)
                     /locus_tag="PK812_08640"
     CDS             complement(380..1381)
                     /locus_tag="PK812_08640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRJ69662.1"
                     /translation="MSAAPRVTIVVPAYNAADCIGAALDSVLAQSMPDFEVIVVDDGS
                     RDATVAVVQNHARRDPRVVLVRQERNGGVSAARNAALDRARGVWITVLDSDDTYRPER
                     LEVLLAAAERHDLAIVADNLLMHDDGSAGDIGPSFAFDGPICRLTRDLLVRNDSPPLI
                     VCLGHLKPMFRRDLIERTGLRYPTDIAVGEDFHFLFCLLGLSQRAALLHHAGYRYTLP
                     FNVLTGSRASGSRTAYGLSSLDTLARSNALLAETLGDSPDTGPLRALLQARGRMFPNE
                     KHWRRLREHVHSRALLAALKTIVRVDPSFTMHQIAAYLRRRGGRFDTVLHGVEPFPS"
     gene            complement(1545..3146)
                     /locus_tag="PK812_08645"
     CDS             complement(1545..3146)
                     /locus_tag="PK812_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69663.1"
                     /translation="MPVSLMFFAATAILFVFQLIPFTGVFLMMLAAPFWSIALVNAGF
                     VGVGVEAALGKVNRAWLLLPLAWFAGYSYFAAQSRQEAERVRQDMLAGNAKGTLAFNP
                     KTDDFVAGEGRADLPSVADTLIANYDIPTVFEKFGSTSGFGHRVRRFATGAVCDQLTE
                     RLKGGLDTNFAVWPFRENNAPVAGACIHYHPGDPGPRAFQMTSAKTAWSRMLARFTVT
                     TLTITAPTGEGVTRSFGQAAVLGWLPMPVIGCALISAGPSWSCTAEFWRSTIRLPANE
                     YEPILAARVVASALGLQPSRAATRVATAAPVLPAQVAKTFEDVEAAALKKLDQVLARD
                     RAALNYTMHDMTPIRDKPALWRSRMDRMADFFVENFNRRADGRGIPEVINTLFEVMPP
                     EDFRRYATRLAEVVRQRPAGACDQGRAVLARIGEAGASALPAIEIVATQNPGRDCMLF
                     LVQSICRIGPAAERMVPYVKTVFGQFTPQHNGHFYFASYRALMRLGHGDYVRSHVQPT
                     RNTREQRLMKELDTITPGSPTTVCV"
     gene            complement(3254..3907)
                     /locus_tag="PK812_08650"
     CDS             complement(3254..3907)
                     /locus_tag="PK812_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRJ69664.1"
                     /translation="MITLYHCESARSFRALWALEELGLPYDLKMLPFPPRYLKREYLE
                     TNPLGTVPLLIDGATRMTESAAICEYLAVRYGNGALAVAPDDADYGAWLNWLHYGEAT
                     LTFPQTLVLRYSRLEPKDRRLPQAAEDYRIWFTKRTLMLEQALADRTYLVGGRFTAAD
                     ISVGYAVMLAELIGLAGDLNPVVRGYFERLSAREAFKRALAAQKVGMQAAGIASWLG"
     gene            complement(4054..4812)
                     /gene="tpiA"
                     /locus_tag="PK812_08655"
     CDS             complement(4054..4812)
                     /gene="tpiA"
                     /locus_tag="PK812_08655"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014285909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRJ69665.1"
                     /translation="MSNRPKPLVAGNWKMNGLRAALKELGEMGQGHDAALRAKCDLLI
                     CPPAHLVYVAAAAAIGTRIAIGGQDCHAKASGAHTGDISAEMLADSGATFCIVGHSER
                     RADHGESNAAVKAKAEAALRAGLTAIVCVGETKDERVSGKTLAVVRRQVRNSLPDGAS
                     AANTVIAYEPVWAIGTGLTPTVADVAEVHADIRRTLKSLMKGEAAGVRILYGGSVKPD
                     NAKALLSVDNVDGALVGGASLKAADFLAIAAAYR"
     gene            4992..6917
                     /locus_tag="PK812_08660"
     CDS             4992..6917
                     /locus_tag="PK812_08660"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HRJ69666.1"
                     /translation="MLDGIRKAAQNWLGRIVLIFIMSILILSFAIWGIGDMLRVSGVN
                     TVANVGGVEINVEQVRRAWMNSLEDLSNRARRRITSDEAKQLGLDRQVISRMVSEALL
                     DQRARKLGLNVSEANVIASITSDRTFQGPDGKFDRLRFYDVLQRNGYSEAAFAAEQKK
                     SILRRQIAVGVAGEVEPPRALLDAAYRYATEERTVDYFLLTPSMAGEVAAPEDAVLKE
                     FYENRKFEFRAPEYRKVNLVTALPSELGVEITVTDDDLKAVYDRGLAAGRFGTLARRQ
                     VQQILIPTEMEAAAAALRAQTAGGFDAVLAERKIKPEDADLGFKTRAQFADPAIAAAV
                     FALKEGEVSIPVRTNFGFALLRLVKIDPGTEQPFEAVKASLADEARLDKLRRDSRIQS
                     RLDDIQKKIEDARTNGKSLAEAAPLAGLSVKAIDALDAKGLDKAGVKVDLPGADETQR
                     AIFQSDIGLDNEALRLREGGLVWFEIAGVESARDRPFDEVKAEVLLRWKTEEAAKRLR
                     EKAEALVKRLDAGEDFGIVASDTSVESIETKITRSASGSLGAAGSAQAFAVPVGRSVS
                     AATPEGGRLVIRVKDASAKAFEPDSPVGQNLRRQLSEQLGEDIVTQYIQKLQVEIGAT
                     INQRALQSAIGGGTTNN"
     gene            complement(7180..7992)
                     /gene="tatC"
                     /locus_tag="PK812_08665"
     CDS             complement(7180..7992)
                     /gene="tatC"
                     /locus_tag="PK812_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HRJ69667.1"
                     /translation="MSTEVDNPRAGQEDIDASKAPLMEHLVELRSRLIKAALAFLVMT
                     IFCFAVAKYIYRILVWPYIWAAGGPENAPLIYTHPLEYFFVQLKLALFGGAFLAFPVI
                     ATQIYKFVAPGLYKDERDAFRPFLIATPVLFVLGAIVVMVMAMPLLMRFSLAMQQLDA
                     GDAKISLLPKVDDYLSLIMTLIFAFGITFQLPVILTLLGRVGLIDAQFLREKRRYAIV
                     IVFVIAAILTPPDVISQLALAIPAMLLYELAIFAVLWAEKKHKSGRELDTPG"
BASE COUNT         1423 a         2659 c         2704 g         1270 t
ORIGIN      
        1 tcgatgtcgg cgttgacgaa attcaccgtc accggcgccc gcgagcgcgc ggccatgatc
       61 gacaggatca ggcttgtggc gaaaaacgcc gcgccgagaa tggcagtggt gcgcgtcagc
      121 aggttcgcct gcccgcgacc ggtcaggaaa ccggaggggg tgccggagcc accgcccatg
      181 ccgaggcccc cgccttcgga gcgctgaagc agcaccgtgc cgcagagcgc gacgacgatg
      241 atcaggtgaa tgacgatcag aacggtatcc atcggagctt tcgtgccgga gcgtgcgtga
      301 actgtggaat tgggcgctga ctagagcatt ttcaagccgt ggggaagccc tcgttgcggg
      361 gcggtgcgca cggacgccct caggacggga acggctcgac gccatgcagc acggtatcga
      421 accgcccgcc ccgacgacgc agatacgcgg cgatctggtg catcgtgaag gacggatcga
      481 ccctcacgat ggtcttgagc gccgcgagaa gcgcgcggga atggacatgc tcgcgcaggc
      541 gccgccagtg tttttcgttc gggaacatcc ggccacgcgc ctgcagcagc gcccgcagcg
      601 gaccggtgtc cggcgagtcg ccaagggttt cagccagcag cgcgttcgag cgcgccagcg
      661 tgtcgaggct ggaaaggccg taggccgtgc gcgatccgga ggcgcgcgac ccggtcagga
      721 cgttgaacgg cagggtgtag cggtagcctg cgtgatgcag cagcgccgcg cgctgcgata
      781 atccgagcag gcagaacaga aaatggaaat cctccccgac cgcgatgtcc gtggggtagc
      841 gcagcccggt acgctcgatc aggtcgcgac ggaacatcgg cttgagatgg ccaaggcaga
      901 cgatcagcgg cggcgaatca ttgcgcacca gcaggtcccg cgtcagccgg cagatcgggc
      961 catcgaaggc aaaggacggt ccgatgtcgc cggcgctgcc gtcgtcatgc atcagcaggt
     1021 tgtcggcgac gatggcaagg tcatgacgct cggccgcggc caagagcact tcaagccgct
     1081 ccggccggta ggtgtcgtcg ctgtcgagca cggtgatcca gacgccgcgc gcccggtcca
     1141 gcgcggcgtt tcgcgccgcc gacacaccgc cattgcgttc ctgccggacc aggacaacgc
     1201 ggggatcgcg ccgcgcatgg ttctgcacga ccgcgaccgt ggcgtcacgc gacccgtcgt
     1261 ccacgacgat cacctcgaag tccggcatcg attgcgccag caccgagtcc agcgcggcac
     1321 cgatgcagtc ggcggcattg taggccggaa caacaatggt gacgcgcggc gctgcggaca
     1381 ttccaaacct gtcgaacgtg ccggggattt ggccatgccg gcctaaagga ttgctgaaag
     1441 tcgccggaaa ccggttcggg cgagcccaag cccggcgccc tgcgacgggg ccatgcctga
     1501 cgaaggaacc ggagcggcgc ccccgcgccg cgccgcccgc gatctcagac gcagaccgtg
     1561 gtgggactgc cgggggtgat cgtgtccagc tccttcatca gcctctgctc tcgggtgttg
     1621 cgggtgggct ggacgtgact gcgcacgtag tcgccatggc caaggcgcat cagggcgcgg
     1681 taggatgcga agtagaagtg cccgttgtgc tgcggcgtga actggccgaa gaccgtcttg
     1741 acatagggca ccattcgctc ggctgcaggc ccgatgcggc agatggattg gaccaggaag
     1801 agcatgcaat cgcgcccggg gttctgcgtc gccacgattt cgatcgctgg taatgccgat
     1861 gcgccggcct cgccgatgcg cgccagaacc gctctgccct gatcgcaggc gcctgcagga
     1921 cgctggcgca cgacctcggc cagtcgtgtg gcatagcgcc tgaaatcctc agggggcatg
     1981 acttcgaaca gggtgttgat gacctcaggg atgcccctgc cgtccgcgcg tcggttgaag
     2041 ttctcgacaa agaaatccgc catgcgatcc atgcgcgagc gccagagcgc cggcttgtca
     2101 cggatcggcg tcatgtcgtg catggtgtag ttgagcgcgg cacgatcgcg tgcgagcacc
     2161 tggtccagct ttttcagcgc tgcggcctcg acatcctcga acgtcttcgc cacctgcgcc
     2221 ggcaggacgg gagccgctgt ggcgacccgg gttgccgccc gcgacggctg aaggcccagc
     2281 gcgctggcaa ctaccctggc ggcgaggatg ggctcgtatt cgtttgctgg gagcctgatc
     2341 gtcgagcgcc agaattcggc cgtgcatgac cagctgggtc cggcggagat gagcgcacag
     2401 ccgatcaccg gcatcggcag ccagccgagc actgccgcct ggccaaagga ccgcgtcacc
     2461 ccttctcccg tcggcgctgt gatggtgaga gtcgtgacgg tgaagcgagc cagcatgcga
     2521 gaccaagccg ttttcgccga tgtcatctgg aaagcgcgtg gtcccggatc gcccgggtga
     2581 tagtggatgc aggcgcccgc gacaggcgcg ttgttctcac ggaaaggcca aacggcgaaa
     2641 ttggtgtcaa ggccgccctt gagccgttcg gtcagctggt cgcacaccgc gccggtagcg
     2701 aacctgcgga cacggtggcc gaagccgctg gtgctgccga acttctcgaa gaccgtcgga
     2761 atgtcgtagt tcgcaatcag cgtgtccgcg acggagggca gatccgcccg gccttcgccc
     2821 gcgacgaagt cgtccgtctt cggattgaac gccagtgtgc ccttggcatt gccggccagc
     2881 atgtcctgac ggactcgctc cgcttcctga cgggattgcg ccgcgaaata ggaatagccg
     2941 gcgaaccagg ccagcggcag cagcagccag gcccggttga ccttgccgag cgccgcctcg
     3001 acgccgacgc cgacgaaccc ggcattgacc agcgcgatcg accagaacgg cgccgccagc
     3061 atcatgagga agacgccggt gaacgggatc agctggaaaa cgaagaggat cgccgtggcg
     3121 gcgaagaaca tcagggaaac aggcatgcgc actccggcag agtgcccgca ctctcgcgcg
     3181 ccatccttgg cgcatcctta agccggcccc atgacccgcc gcgatttccg aacctacggt
     3241 taccggatgg ctgtcagccg agccagctcg cgatgcctgc ggcctgcatg ccgaccttct
     3301 gggccgcaag ggcgcgcttg aacgcctcgc gcgcgctgag ccgctcgaaa tagccgcgca
     3361 ccaccgggtt gaggtcgccg gcaaggccga tcagttcggc cagcatcacc gcatacccca
     3421 ccgaaatgtc ggccgcggtg aaccgcccac cgaccagata ggtccggtcc gccagggcct
     3481 gctccagcat caaggtccgc ttcgtgaacc agatgcggta atcctcagcc gcctgcggca
     3541 ggcgcctgtc cttcggctca agccgcgaat agcgcagcac cagcgtctgc ggaaaggtca
     3601 gcgtcgcctc gccgtaatgc agccagttga gccaggcgcc gtagtccgcg tcgtccggcg
     3661 cgaccgcgag cgcgccgttg ccatagcgga cggccagata ctcgcagatc gccgccgatt
     3721 ccgtcatccg cgtcgcaccg tcgatcagca acggcacggt tccgagcggg ttcgtctcca
     3781 gatactcgcg cttgaggtag cgcggcggaa acggcaacat cttcaggtcg taaggcaggc
     3841 cgagctcttc cagcgcccac agcgcccgga acgaacgggc ggactcgcag tgatacagcg
     3901 tgatcatgtc cgccccctct cccgctccgg gccacgactt atgccaaggg tcatgacaat
     3961 ggacccttgg ttcctttcct gaattttcct tgcgcattgt aaaacaagga aaattcaaga
     4021 gcggtcatcg gtcatccctg atgaccgatg gtattaccgg taggcggcgg cgatcgccag
     4081 gaagtcggcc gccttgaggc tcgcgccgcc gacaagcgcg ccgtcgacgt tgtcgaccga
     4141 gagcagcgcc ttggcgttgt ccggcttcac cgagccgcca tagaggatgc gcacgcccgc
     4201 ggcctcgccc ttcatgaggg acttcagcgt ccggcgaatg tcggcatgga cttccgcgac
     4261 gtccgcgacc gtgggcgtca gcccggtgcc gatggcccag accggctcgt aggcgatcac
     4321 ggtgttggct gcgctcgcgc catccggcag cgagttcctg acctgccggc gcaccaccgc
     4381 gagggtcttg ccggagacgc gttcgtcctt ggtctcgccg acgcagacga tggccgtgag
     4441 gcccgcccgc agcgccgcct cggccttggc cttgacggcg gcattgctct cgccgtgatc
     4501 ggcgcgacgc tcggaatggc cgacgatgca gaaggtcgcg ccggaatccg ccagcatctc
     4561 agcggaaatg tcgccggtat gcgcgcccga ggccttggcg tggcaatcct gcccgccgat
     4621 ggcgatgcgt gtgccgatgg cggcggccgc cgccacgtac accagatggg ccggcgggca
     4681 gatcagcagg tcgcatttcg cccgcagcgc ggcgtcatga ccctgcccca tttcgccaag
     4741 ttccttgagc gcggcgcgca ggccgttcat cttccagttg ccggcgacga gcggtttggg
     4801 gcggttggac atgggatctc cggacgggtc ggttggcctg gacccgcgcc atacagccga
     4861 cgcgcgccgc ctgcaatcgt ccggttgcga agcggtaact gcatctttat ggtgcgcgct
     4921 cttttcaaga ggcccgattt cgggcatcgc accggcacgc ttcgacagac agccaggaca
     4981 gtacaccgcc catgctcgac ggaattcgca aggccgcaca gaactggctc ggccgcatcg
     5041 tgctcatctt catcatgagc atcctgatcc tgtccttcgc gatctggggc atcggcgaca
     5101 tgctgcgcgt cagcggcgtg aacaccgtgg ccaatgtcgg cggcgtggag atcaacgtcg
     5161 agcaggtgcg ccgcgcctgg atgaattcgc tggaagacct gtcgaaccgc gcacgccgcc
     5221 gcatcacgtc ggatgaggcc aagcagctgg gcctcgatcg gcaggtgatc tcgcgcatgg
     5281 tcagcgaggc gttgctggac cagcgtgcca ggaagctcgg gctcaacgtg tccgaggcca
     5341 acgtcatcgc ctcgatcacc tcggaccgca ccttccaggg gccggacggg aagttcgaca
     5401 ggttgcgctt ctacgacgtg ttgcagcgca acggctacag cgaggccgcc ttcgccgccg
     5461 aacagaagaa atcgatcctg cgccggcaga tcgcggttgg cgtcgccggc gaggtggagc
     5521 cgccgcgtgc gctgctcgac gccgcctacc gctatgcgac cgaagagcgc acggtggact
     5581 atttcctgct gaccccgtcc atggcgggcg aggtcgcggc gcccgaggat gccgtgctca
     5641 aggagtttta cgagaaccgc aagttcgagt tccgcgcgcc ggaataccgc aaggtcaacc
     5701 ttgtcaccgc cctgccgtcc gagctcggcg tcgagatcac cgtcaccgac gacgacctga
     5761 aggcggtcta tgaccggggg cttgccgccg gccgcttcgg cacgctggcc aggcgccagg
     5821 tgcagcagat cctgatcccg accgaaatgg aggctgcggc cgccgcgctc cgggcccaga
     5881 ccgccggcgg cttcgacgcc gtcctcgccg agcgcaagat caagccggag gacgccgatc
     5941 tcggcttcaa gacccgcgcg caattcgccg atcccgccat cgccgccgcc gtcttcgcgc
     6001 tcaaggaggg tgaggtcagc atcccggtca ggacgaactt cggcttcgcg ctgctgcgtc
     6061 tggtgaagat cgatcccggc accgagcagc ccttcgaggc agtgaaggcc agcctcgcgg
     6121 atgaagcccg tctcgacaag ctgcgtcgcg attcccgcat ccagtcgcgg ctcgatgaca
     6181 tccagaagaa gatcgaggac gcgcgcacta acggcaagtc cctcgccgag gctgcgccgc
     6241 tcgccggtct ctcggtcaag gccatcgacg cgcttgacgc caagggcctc gacaaggccg
     6301 gcgtgaaggt cgacctgccg ggcgccgacg agacgcagcg ggccatcttc cagtccgaca
     6361 tcggcctcga caatgaggcg ttgcgcctgc gcgagggggg actggtctgg ttcgagatcg
     6421 ccggcgtcga gtccgcccgc gaccggccgt tcgacgaagt gaaggccgag gtcctgctgc
     6481 gctggaagac ggaggaggcc gcaaagcgcc tcagggaaaa ggcggaagcc ctggtcaagc
     6541 gtctcgacgc gggcgaggac ttcggcatcg tcgcctcgga cacgagtgtc gagagcatcg
     6601 agaccaagat cacccgctcc gcctccggct cgctcggcgc ggccggttcg gcacaggcct
     6661 ttgccgtgcc ggtcggacgg tcggtttccg ccgccacgcc cgaaggcggc cgtctggtga
     6721 tccgggtgaa ggacgcctcg gccaaggcct tcgagcccga cagcccggtg ggccagaacc
     6781 tgcgccgtca gctctccgag cagcttggcg aggatatcgt cacgcagtac atccagaagc
     6841 ttcaggtcga gatcggcgca acgatcaacc agcgcgccct gcagtcggcc atcggcggcg
     6901 gcaccacgaa caactagggg aagtctgttc gcgggctgtc cggcgattgg tgcactccag
     6961 cgctgcacgc ctgtgcgatg ccggctgttt ctgcgctgaa ggggcaagta ggcccttagc
     7021 aagcccctgg ctctcgggtg ccgcgcggcc gtcagaagcg cgctgcttgt ggcagcccgg
     7081 gcccgcaagt cctgataagc ccgggtttga aaacccgtgt cagacgcgcg ccggcctgtg
     7141 cgaagccttg ccgggccagc gcctggggcc gcgccgcgct caccccggag tgtcgagctc
     7201 gcgcccggac ttgtgcttct tctccgccca cagcaccgcg aagatcgcca gctcgtagag
     7261 cagcatcgcg gggatcgcca gcgcaagctg gctgatcaca tcgggcggcg tcaggatcgc
     7321 agcgatgacg aagacgatga cgatggcgta gcgccgcttt tcccgcagga actgcgcgtc
     7381 gatgaggccc acccgcccga gcaacgtcag gatcaccggc agctggaagg tgatgccgaa
     7441 ggcgaagatc agcgtcatga tcagcgacag gtaatcgtcc acctttggca gcagcgaaat
     7501 cttcgcatcg cccgcatcga gttgctgcat cgccagcgag aagcgcatca gcagcggcat
     7561 ggccatgacc atgaccacaa tggcgccgag cacgaacagc accggcgtgg cgatcaggaa
     7621 cgggcggaag gcgtcgcgct cgtccttgta aagccccggc gcgacgaact tgtagatctg
     7681 cgtcgcgatc accggaaagg ccaggaaggc gccgccgaac agcgccagct tgagctggac
     7741 gaagaagtac tccagcggat gcgtgtagat cagcggggcg ttctccggcc cgccggcggc
     7801 ccagatgtag ggccacacca gaatgcggta gatgtacttc gccacggcga agcagaagat
     7861 ggtcatgacc aggaaggcga gcgccgcctt gatcagccgc gagcgcagct cgaccagatg
     7921 ttccatcaac ggcgccttgg aagcgtcgat gtcctcctgg ccggcgcgcg ggttgtcgac
     7981 ttcggtgctc atgcggcggt gctgttcgtc gtgctcgtgg cgggcgtcgc ggtcgcgatc
     8041 tggtccgtcg gcggtt
//
LOCUS       DAOMXN010000110         8017 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_4716_length_8017_cov_11.907561, whole genome shotgun sequence.
ACCESSION   DAOMXN010000110 DAOMXN010000000
VERSION     DAOMXN010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 8017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8017
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4716_length_8017_cov_11.907561"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..748)
                     /locus_tag="PK812_08670"
     CDS             complement(53..748)
                     /locus_tag="PK812_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HRJ69668.1"
                     /translation="MTPPAALFFDIFGTVVDWRGTIAREAPAVAGRTFDSAAFADAWR
                     AEYQPAMERIRSGGRGYVKLDILHRENLERILPRFGLDDLPEDKRAALNRLWHRLDGW
                     PDARTGLERLRTKFLIAPMSNGNISLMVDMARHNGFAWDAILGSELARDYKPKLEVYR
                     AGCAALDLPPERCMMVAAHTDDLAAAAAAGLRTAHVARPDEHGPGRGETGPGALVDIA
                     ARDLVDLAAKLGA"
     gene            complement(878..1864)
                     /gene="cobS"
                     /locus_tag="PK812_08675"
     CDS             complement(878..1864)
                     /gene="cobS"
                     /locus_tag="PK812_08675"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008192290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobS"
                     /protein_id="HRJ69669.1"
                     /translation="MSSTTTAGDGLPDIKISVRTAFGIDSDMQVPGFSAGSEHVPDLD
                     PDYLFDRDTTMAILAGFAHNRRVIVTGYHGTGKSTHIEQVAARLRWPCVRVNLDSHVS
                     RIDLVGKDAIVLKDGKQVTEFRDGILPWAYQHNVALVFDEYDAGRPDVMFVIQRVLES
                     SGRLTLLDQSRVIRPHAAFRLFATANTVGLGDTSGLYHGTQQINQAQMDRWNIVTTLN
                     YLPHDNEVGIVLAKAKHYQNKEGKDIVNKMVRVADLTRNAFINGELSTVMSPRTVITW
                     AENADIFKDVAFAFRLTFLNKCDELERSIVAEFYQRCFGVELKESSVNVVMT"
     gene            complement(1952..2536)
                     /locus_tag="PK812_08680"
     CDS             complement(1952..2536)
                     /locus_tag="PK812_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015823431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HRJ69670.1"
                     /translation="MDFNSPLFDRIRICQPAEEPAERGCDHAGCTRPGAYRAPQGRNR
                     EGRYFHFCLDHVRAYNQTYNYFKDMPDEAVRAYQKEAVTGHRPTWTMGTKAAERSGGP
                     TVDSLGDPFEMFGNFRPQARPAEPKRRVSAGAAKALDKLGLDETATKEQVRAQFKLLA
                     KRFHPDLNGGDRSREEKLRSIIDAYNYLKSAGLA"
     gene            2612..2878
                     /locus_tag="PK812_08685"
     CDS             2612..2878
                     /locus_tag="PK812_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HRJ69671.1"
                     /translation="MTMKERIIRNLTVGLSPAELEVVDDSHKHHGHSGWREGGETHFR
                     VRIVSSRFTGLGRVQRHRLVNELLAAELKERVHALALETKAPGE"
     gene            2979..4265
                     /locus_tag="PK812_08690"
     CDS             2979..4265
                     /locus_tag="PK812_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRJ69672.1"
                     /translation="MLGLEVLFLLALTVLNGFFAMSEMAIVSSRRARLEAMAEAGDAG
                     ARKAAELAEEPSSFLSSVQIGLTLIGIGTGAFGTATLAEKLALPISSIAWLAPHKEAV
                     AIGLVVVVTTVLTLVFGELVPKRIALAYPEPIAARVARPLSAIALAARPIVAFLGAAT
                     TGTLRLLRIKTVRDQSVTEEEVETMLQEGTESGVIDPAEREMVKEVIGLAERPVLAIM
                     TRRTEVYWVDVADDPKVLRAELRQCPYSRVVVAKGGSIDEPLGYVHKKDLLDLLLEGK
                     PLDVEKNVRQPIFVPEAASVLDVLELFRSKRNHFAFVIDEFGSFEGVVSLTDVLEAIT
                     GDIPEEHEETRQSIIARPDGTYLVDGRAEIRELEETLGVPIAEDARFHTVAGLALEVF
                     GRIPVEGETVRLDEWQIEIIDMDGRRIDKLLFTRNA"
     gene            complement(4288..5481)
                     /locus_tag="PK812_08695"
     CDS             complement(4288..5481)
                     /locus_tag="PK812_08695"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRJ69673.1"
                     /translation="MIILPVFLFAYIFSQFYRSFLAVVTADLTRDLGVGPAELGNLSA
                     IWFVVFSLAQFPVGYLLDRHGPRRTISAMMLAAVAGAVLFASARSFPVALAGMGLIGL
                     GCAPILMGSLYYFGRNSGPEKFPIYTSIMVGIGNLGNLLGGRPLSQAVEAFGWRASLL
                     AIAALTLLSAAMVAFFVRDPPRLEAPGRSGNALTELWEIVSYRPLWPIFLLNFASYGV
                     VAAERGLWLGPYLQDVHGLEALARGNAAFLMALGMAIGAFVAGWMVPWFGSSKRVAIV
                     GNLGTAAAFLGLGLIPGLGAPGAVALQVLAGLFGMCYGVILSHARLFFPEHLLGRGVT
                     FANFLSIGGAGLVLSGSGYFMRGALADGISPTAAFSMLHVSFAAMLLTALVFYVRSPA
                     GPEKR"
     gene            complement(5516..6682)
                     /locus_tag="PK812_08700"
     CDS             complement(5516..6682)
                     /locus_tag="PK812_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HRJ69674.1"
                     /translation="MTACIVGWAHTPFGKMDAETVESLIVRAATDALAHAGIGPADVD
                     EIVLGHFNGGFSAQDFTAPLVLQADPALRFKPATRVENACATGTAAIHQGIKAIAARA
                     SRVVLVVGVEQMTRTPGPEIGKTLLKASYLPEDGDTPGGFAGVFGKIAQSYFQKYGDN
                     SDALALIAAKNHKNGVANPYAQMRKDFGFEFCRTESDKNPFVAGPLKRTDCSLVSDGA
                     AAVVLADVETALGMKRAIAFRSVAHAQDFLPMSKRDILQFEGCSVAWKRALDTARLTL
                     DDLSLVETHDCFTIAELIEYEAMGLTRPGEGARAIKEGWTHKDGKLPVNASGGLKSKG
                     HPIGATGVSMHAMTAMQLMGEAGDMQVKNATIGGVFNMGGAAVANYVSVLERLR"
     gene            6905..>8017
                     /locus_tag="PK812_08705"
     CDS             6905..>8017
                     /locus_tag="PK812_08705"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HRJ69675.1"
                     /translation="MTGARFLGGLIALGLTLGVPAAHAAPSSPELVIDVATGQVLLAD
                     EATRPWFPASTTKMMTAYVVLRAVKAGELKLETPLIASAKAAAQPPSKIGIRPGQEIT
                     VDNALKILMVKSANDLAQVIAEGVGGSQEAFAQRMNAEAARLGMRDSHFVNPHGLFHA
                     AQVSSARDLAILGRAMLVEFPEYRDYWGIGAVQLGQRVMKNTNGLIGRYPGAEGMKTG
                     FICPSGFNVVATASRGGRTLLAVILGAGSGAERSIRTAQILDRGFASSAWAPSLGSVT
                     TLPPSGHASAPNMRQDICGRGRQVAFEEEDTGKAISLPITATGDNASVYAIMTPSPMP
                     GASSSVGGKNASGRAVLGPRAELPPILVHLGRGPGNT"
BASE COUNT         1416 a         2657 c         2626 g         1318 t
ORIGIN      
        1 gcttgcgggc gccggctggc ccggcttgcg gttggtggtg gtcatggaga agtcatgccc
       61 cgagtttggc cgcgagatca accagatcac gcgccgcgat gtccaccaat gcgcctggcc
      121 ccgtctcgcc gcgccccgga ccatgctcgt caggccgtgc gacatgggcc gtcctgaggc
      181 cggcggcagc ggcggcggcg aggtcgtcgg tatgggccgc gaccatcatg cagcgctccg
      241 gcggcagatc cagcgctgcg cagccggcgc gatagacctc aagcttgggc ttgtagtcgc
      301 gggcgagttc cgagcccagg atcgcatccc aggcaaagcc gttgtgccgc gccatgtcga
      361 ccatcagcga aatattgccg ttggacatcg gcgctatcag gaatttcgtc cgaagtcgct
      421 ccagaccggt ccgcgcatcg ggccatccgt cgaggcggtg ccagagccgg ttcagcgccg
      481 cccgcttgtc ctccggcagg tcgtcaaggc caaagcgcgg caggatgcgt tcgagattct
      541 cgcggtgcag gatatcgagc ttcacatagc cgcgcccgcc ggaacggata cgctccatcg
      601 ccggctggta ttcggcccgc caggcatcgg cgaaagcggc gctgtcgaag gttcgcccgg
      661 caacggccgg tgcttcccgc gcgatggtgc cgcgccagtc cacgaccgtg ccgaaaatgt
      721 cgaagaacag ggcagcagga ggggtcatcg gtcgtcggcc gtcattgcga ggagcgtctg
      781 cgacgaaaac aatccggaca caccgagcca aaagtccgga tggcttcgtc ggccaccggc
      841 ctccgcgcaa tgacaggagg aggccgggcc cagggtttta cgtcatcacc acgttcaccg
      901 agctttcctt cagctcgacg ccgaagcagc gctggtagaa ctcggcgacg atggaacgct
      961 ccagctcgtc gcacttgttg aggaaggtca ggcggaaggc gaaggccaca tccttgaaga
     1021 tgtcggcgtt ctccgcccag gtgatcaccg tgcgcggcga catgaccgtg gacaactcgc
     1081 cgttgatgaa ggcgttgcgg gtcaggtccg ccacgcgcac catcttgttg acgatgtcct
     1141 tgccttcctt gttctggtag tgtttggcct tggccaggac gatgccaacc tcgttgtcat
     1201 gcggcaggta gttcagggtc gtcacgatgt tccagcggtc catctgcgcc tgattgatct
     1261 gctgcgtgcc gtggtagagg cccgaggtgt cgccgaggcc gaccgtgttg gcggtcgcga
     1321 acagtcggaa cgcggcatga gggcggatca cccggctctg gtcgagcagg gtcaggcggc
     1381 cggaggattc cagcacgcgc tggatcacga acatcacgtc cgggcgaccg gcgtcgtatt
     1441 cgtcgaacac gagcgccacg ttgtgctggt aggcccaggg caggatgccg tcgcggaact
     1501 ccgtgacctg cttgccgtcc ttcagcacga tcgcgtcctt gccgacgaga tcgatacgcg
     1561 agacgtggct gtcgaggttg acgcgcacgc agggccacct gagccgcgct gccacctgct
     1621 cgatatgggt cgacttgccg gtgccgtggt agcccgtgac gatcacgcgc cggttgtgcg
     1681 cgaagcccgc caggatcgcc atcgtagtgt cgcggtcgaa cagatagtcc ggatcgagat
     1741 cggggacatg ctcgctgccg gcggaaaagc cgggcacctg catgtcgctg tcgatgccga
     1801 aggccgtccg gaccgaaatc ttgatatcgg gcaatccgtc gcccgcagtc gtcgtcgaag
     1861 acatgtcaca cctatccacc gccccgttcc ggattccgga cgggaaggcg cgtagcaaag
     1921 atcattgggg cgcggaagga agtctggcga cctaggcgag cccagccgat ttgagatagt
     1981 tataggcatc gatgatcgag cgcaattttt cttcgcgcga ccggtcgcca ccgttgagat
     2041 cggggtggaa gcgcttggcc agcagcttga actgcgcgcg cacctgctcc ttcgtggcgg
     2101 tctcgtcgag accgagcttg tccagcgcct tggcagcgcc tgcgctgacc cgacgcttcg
     2161 gctcggcggg acgcgcctgc ggccggaaat tgccgaacat ctcgaaggga tcgccgagcg
     2221 aatcgacggt cgggccgccg ctgcgttcgg cggccttggt gcccatggtc caagtcggcc
     2281 ggtgcccggt caccgcttcc ttctggtagg cgcgcacggc ttcgtccggc atatccttga
     2341 agtaattgta cgtctggttg taggcgcgca cgtgatccag gcagaagtgg aaatagcgcc
     2401 cctcgcgatt gcgaccctgc ggcgcccgat aggcgccggg ccgcgtgcag cccgcatgat
     2461 cgcagccgcg ctccgccggc tcttccgcag gctggcagat gcgaatgcga tcaaacaggg
     2521 gcgaattgaa atccatcgtc tgagaatagg catcgcgccg gttgccggca agcgccgggc
     2581 atgggaaaga ggcgacaagg agctatgcct gatgacgatg aaggaacgca taatccgcaa
     2641 cctgacggtg ggactctcgc ccgccgaact cgaagtcgtt gacgacagcc acaagcacca
     2701 cggccattca ggctggcgcg agggcggcga gacgcatttt cgcgtccgca tcgtctcctc
     2761 gcgcttcacc ggtcttggcc gcgtgcagcg ccatcggctg gtcaacgagt tgctggcggc
     2821 ggagttgaag gagcgtgtcc acgcgctcgc ccttgagacc aaggcccccg gggagtaatc
     2881 tccgccgaga atgttggcgc cgtcgcattg ccggatgagc ccctcctatg cgataaggcg
     2941 acgcggccat tgcatgccgc gcaacttcac taagtttgat gctgggcctc gaagtccttt
     3001 ttctcctcgc cttgaccgtt ctcaacgggt tcttcgcaat gtcggagatg gcgatcgtat
     3061 catcccggcg ggcgcggctg gaagcgatgg cggaagccgg agatgcgggg gcgcgcaagg
     3121 cggctgaact cgccgaggaa ccgtccagct tcctgtcatc cgtgcagatc ggcctgacac
     3181 tcatcggcat cggcaccggc gcgttcggca cggccaccct ggccgagaag ctggcgctgc
     3241 cgatcagctc gatcgcctgg ctggcgcctc acaaggaggc cgtggccatc ggccttgtcg
     3301 tggtggtgac gaccgtcctg acgctggtct tcggcgaact ggtgcccaag cgcatcgccc
     3361 tggcctatcc cgagcccatc gcggcgcgcg tcgcccgtcc gctgagcgcg atcgcgctgg
     3421 ccgcgcgccc gatcgtcgcg tttcttggcg ccgccaccac cggaacgctc cggctgctgc
     3481 gaatcaagac ggtgcgcgac cagagcgtca cggaggaaga agtcgagacc atgctccagg
     3541 agggcacgga atccggcgtg atcgacccgg cggagcggga aatggtgaag gaggtcatcg
     3601 gcctggccga gcggccggtc cttgccatca tgacgcgccg caccgaggtc tactgggttg
     3661 acgtcgcgga tgatccgaag gtgctgcgcg ccgagctgcg gcagtgcccc tattcgcgcg
     3721 tcgttgtcgc caagggcgga tcgatcgacg agccgctggg ctacgtgcac aagaaggatc
     3781 tgctcgacct gctcctggaa ggcaagccgc ttgacgtcga gaagaacgtg cgccagccga
     3841 tcttcgtgcc cgaggccgcc tcggtcctcg acgtgctcga actgttccgc tccaagcgga
     3901 accacttcgc cttcgtcatc gacgagttcg gatcgttcga gggcgtggtg agcctgaccg
     3961 acgttctgga agccatcact ggcgacattc ccgaggaaca cgaggaaacc cgccagtcca
     4021 tcatcgcccg gccggacggc acctacctcg tcgacggacg cgccgaaatc cgcgaactcg
     4081 aagagacgct cggcgtgccc atcgccgagg atgcgcggtt ccacaccgtc gccggcctgg
     4141 cgctcgaagt gttcgggcga atcccggtcg agggcgagac ggtccgtctc gatgagtggc
     4201 agatcgagat catcgacatg gatggccggc ggatcgacaa gctgctgttc acgcgcaacg
     4261 cctgacgtgg ggaccggcaa ggacggttca gcgcttctcc ggtcccgccg gcgagcgcac
     4321 atagaatacc agcgcggtca gcagcatggc ggcaaacgac acatgcagca tcgagaaggc
     4381 cgctgtcggt gaaatgccgt ccgccagcgc gccgcgcatg aaatagccgg acccggaaag
     4441 caccagcccg gcgccgccga tcgacaggaa attggcgaag gtcacgccgc gtccgagcag
     4501 atgctcaggg aaaaacagcc gggcatgcga caggatcacg ccgtagcaca tgccgaatag
     4561 gccggcgagg acctgcaggg cgacggcccc cggcgcgcca aggcccggaa tcagccccag
     4621 accgaggaag gcggcggccg tgccgaggtt gccgacaatg gcgacccgct tcgaactgcc
     4681 gaaccacggc accatccagc ccgcgacaaa cgcgccgatc gccatgccga gcgccatcag
     4741 gaaggccgcg ttgccgcgcg ccagcgcctc cagaccgtgc acgtcctgca ggtatgggcc
     4801 cagccacagc ccgcgctcgg ccgcgaccac gccgtagctg gcaaaattca ataggaagat
     4861 cggccaaagc gggcggtacg agacgatttc ccacagttcc gtgagagcat tgccggagcg
     4921 tcccggcgcc tccagtctcg gcggatcgcg cacgaagaac gccaccatcg cggcactgag
     4981 caaggtaagc gccgcaatgg ccagaaggct tgcgcgccag ccgaaggctt cgaccgcctg
     5041 cgacaggggg cgcccgccaa gcaggttgcc gaggttgccg atgcccacca tgatcgaggt
     5101 gtagatcggg aatttctccg gaccggaatt gcggccgaag tagtagagcg accccatgag
     5161 gattggcgcg cagcccagcc cgatcagtcc catgccggcg agcgccaccg gaaaactcct
     5221 ggcggaggcg aacagcaccg cgcccgcgac cgcggcaagc atcatggccg agatcgtccg
     5281 ccgcggcccg tgccggtcca gcagatagcc caccgggaac tgcgccagcg agaacacaac
     5341 gaaccagatc gccgacaggt tgccgagttc ggccggcccc acgccgagat cccgcgtcag
     5401 atcggcggtc accacggcga ggaaggaccg gtagaactgg ctgaaaatgt aggcgaacag
     5461 gaagacaggc aggatgatca tgggtgtgac ccggccgcaa gatcgcccgg atgcctcacc
     5521 tcaggcgctc gagcacgctg acgtagttcg ccaccgccgc gccgcccatg ttgaacacgc
     5581 cgccgatcgt cgcgttcttc acctgcatgt cgccggcttc gcccatcagc tgcattgcag
     5641 tcatggcgtg catggagacg ccggttgcgc cgatgggatg gcccttggac ttcaacccgc
     5701 ccgatgcgtt gaccggcagc ttgccatcct tgtgcgtcca gccttccttg atggcgcgcg
     5761 cgccctcgcc cggccgggtc aggcccatgg cctcgtactc gatcagctcg gcgatggtga
     5821 agcaatcatg cgtctcgacc agcgacagat cgtccagggt cagtcgcgct gtatcgagcg
     5881 cgcgtttcca ggcgacggaa cagccctcga actgcaggat gtcgcgcttc gacatcggca
     5941 ggaaatcctg cgcatgcgcg accgagcgga aggcgatggc gcgcttcatg cccagcgccg
     6001 tctcgacatc cgccagcacc acggcggcgg cgccgtcgga caccagcgag cagtccgtgc
     6061 gcttgagcgg cccggcgacg aagggattct tgtcgctttc ggtgcggcag aattcgaagc
     6121 cgaaatcctt ccgcatctgc gcgtagggat tggcgacgcc gttcttgtgg ttcttggcgg
     6181 cgatcagcgc cagcgcatcg gaattgtcgc catacttctg gaaataggac tgcgcgatct
     6241 tgccgaacac gccggcaaag ccgccgggcg tgtcgccatc ttccggcaga tacgaggcct
     6301 tcagcagcgt cttgccgatt tccggtcccg gcgtccgggt catctgctcg acaccgacca
     6361 ccagaacgac gcgggaggcg cgcgccgcga tcgccttgat gccctgatgg atcgcggcgg
     6421 taccggtggc gcaggcgttc tcgacgcgcg tcgccggctt gaagcgcagg gccggatcgg
     6481 cctgaagcac cagcggggcg gtgaaatcct gcgcgctgaa gccgccgttg aaatggccca
     6541 gcacgatctc atcgacatcc gccggtccga tcccggcatg agccagcgca tcggtcgcgg
     6601 cccggacgat cagactctcg acagtctcgg cgtccatctt tccaaatggg gtatgggccc
     6661 agccgacaat gcaggcggtc atctcgatct ccagtcgaag cggttttgat ctggcgccat
     6721 gatttggccc tgttcgccag agtatgggtt tgccgcgatg gttgccagca ctttgtcgcc
     6781 ggtccggcat cgccccgcca acaggaactt ttccctgtcg atcttttcaa cgggccgcaa
     6841 cgcaggtagt cagagcgtct gcgctgaaat tgcccgtcga cgcgccttaa cgggatgaac
     6901 ttgcatgacc ggtgcccgct ttctcggtgg ccttatcgcc cttggtctta cgctgggcgt
     6961 cccggcggcg cacgccgccc cctcctcgcc ggaactggtg attgatgtcg cgaccggcca
     7021 ggtgcttctg gccgacgagg cgacgcggcc ctggttcccc gcctcgacca ccaagatgat
     7081 gacggcctat gtcgtactgc gcgccgtcaa ggccggcgaa ctgaagctgg aaacgccgct
     7141 catcgcctca gccaaggcgg ccgcgcagcc gcccagcaag atcggcatcc gccccgggca
     7201 ggaaatcacc gtcgacaacg cgctgaaaat cctgatggtc aagtccgcca acgatctggc
     7261 gcaggttatc gcggaaggcg tcggcgggtc gcaggaagcc ttcgcccagc ggatgaacgc
     7321 cgaagcggcg cgccttggca tgcgcgacag ccacttcgtc aatccccacg gcctgtttca
     7381 cgccgcccag gtctcctccg cccgtgacct ggcgatcctc ggtcgcgcca tgctcgtgga
     7441 attcccggaa taccgggact actggggcat cggcgcggtc cagctcggcc agcgcgtgat
     7501 gaagaacacc aacggcctga tcgggcgcta tcccggcgcc gagggcatga agaccggctt
     7561 catctgcccc tcgggcttca acgtcgtggc gaccgccagc cgcggcggac gcaccctgct
     7621 cgccgtcatt ctcggggccg ggtccggcgc ggaacgctcg atccgcacgg cgcagattct
     7681 cgacaggggc ttcgccagct cggcctgggc gccgagcctc ggctccgtga ccacgctgcc
     7741 gccctcgggc cacgcctccg cgcccaacat gcgccaggac atctgcgggc gcgggcgtca
     7801 ggtcgcgttc gaggaggagg acaccggcaa ggccatctcg ctgccgatca cggctaccgg
     7861 agacaatgcc tcggtctatg ccatcatgac gccttcgccg atgccaggcg cctccagcag
     7921 cgtcggcggc aagaatgcca gcgggcgcgc cgtacttggc ccccgcgcgg aattgccgcc
     7981 aattcttgtc cacctcgggc gcggtccggg caacacc
//
LOCUS       DAOMXN010000111         7984 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_4740_length_7984_cov_11.100391, whole genome shotgun sequence.
ACCESSION   DAOMXN010000111 DAOMXN010000000
VERSION     DAOMXN010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 7984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7984
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4740_length_7984_cov_11.100391"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="PK812_08710"
     CDS             complement(<1..743)
                     /locus_tag="PK812_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010968521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRJ69676.1"
                     /translation="MKTIRLTMAQALVRAMAAQKTEIDGQIVPLFAGCWAIFGHGNVA
                     ALGEALYGARDVLPTWRAHNEQAMAHAAIAFAKASRRRRMMACTTSIGPGALNMVTAA
                     AVAHVNRLPVLLLPGDVFAGRRPDPVLQQVEDFGDGTVSANDCFRPVSRYFDRITRPE
                     QIIPAFARAMQVLTDPAECGPVTLSLCQDTQAEAFDFPESFFAETVWQPRRHRPDAGE
                     LAAAVSACPNDVGRFCPDQVAQFQVGKKQT"
     gene            complement(837..1256)
                     /locus_tag="PK812_08715"
     CDS             complement(837..1256)
                     /locus_tag="PK812_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HRJ69677.1"
                     /translation="MRENFVEACRRHGLRITGPRKIIAEALSAAHDHPDVRELHRRVM
                     ERDPTVSLSTVYRTLRQFADIGLIEQHTFDGRSVRIERAPTAHHDHLIDTQSGRVIEF
                     SSPEIEALQIEIARRLGYELTGHRLELYGRPLAAKKR"
     gene            1417..2748
                     /locus_tag="PK812_08720"
     CDS             1417..2748
                     /locus_tag="PK812_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009492647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HRJ69678.1"
                     /translation="MLQASGQGWRRAQGEPPLVDVFRSIALLPDASAWRKFLAFLGPG
                     YLVAVGYMDPGNWATSLAGGAQFGYTLLVVALVSNIMAIILQSLCARLAVATNRDLAQ
                     ACRDAYPRWVSWPLWLFAELAICATDLAEVIGTAIALNLLFGIPLEIGVLITAFDVFL
                     ILWLQNKGFRWIEAFIITLLGVIAVCFAIQIALADPNWGEVIRGFAPTTDIVRNPNML
                     FIALGIIGATVMPHNLYLHSGIVQTRAYGGSLDEKRDALKWATVDSTVALMFALTINA
                     SILILAAATFHKAGQTDVAELGRAHELLAPLVGASIAPALFAVALLCCGLNSTVTATM
                     AGQIVMEGFIDIRLPAWLRRLVTRLVAIIPAVVVTLLYGEGQTAKLLILSQVILSFQL
                     PFAIVPLVMFTASREKMGDLVAPRWLTAIAALIAITIIALNVKLLVDVATG"
     gene            complement(2770..3576)
                     /locus_tag="PK812_08725"
     CDS             complement(2770..3576)
                     /locus_tag="PK812_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014529459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRJ69679.1"
                     /translation="MPSPHSRLDGKVAVVTGGTQGLGEAIGREFAARGLKGLVLCGRQ
                     KDKGEAVARDIARTGCRALFVVADLEKTADARAVTRKADAEFGRIDILVNAAGITDRG
                     TIFDTSEDLFDRMVAVNIRSPFFLMQEAAKIMKREKIAGSIINIQSMSANGGQAFLTP
                     YSMSKGALATLTRNVAHALVRHRIRVNGLNIGWMDTPGEDAIMKKYHGAEDGWLEAAA
                     RTRPFGRLIDPKEVARACAYLASDESGLMTGANIDFDQNVVGVGDPPLEP"
     gene            3786..4772
                     /gene="iolG"
                     /locus_tag="PK812_08730"
     CDS             3786..4772
                     /gene="iolG"
                     /locus_tag="PK812_08730"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HRJ69680.1"
                     /translation="MLKVGLLGAGRIGRIHGGNVAVHPRARLVAVADTHAPAAEALAQ
                     ETGAKTASIEAILKDKTIGAVVICTPTDMHADLIEAAAKAKKAVFCEKPVDLNSDRIR
                     ACLKVVARAKTALMIGFNRRFDPNFSALKQRLAGGEVGKVELVTILSRDPGPPPAEYV
                     ARSGGLFRDMMIHDLDMARHLLDEEPVEVHAVGSSLVDKAIGKAGDVDTAAVLLKTKS
                     GKIAQISCSRRATYGYDQRIEVHGSKGLIRAGNVHNTTVEVATGAGFRADPVQNFFLE
                     RYAAAYRNELESFIAAVEKGEAPSPSGEDGLKAQVLADAATEACQTGKTVRL"
     gene            complement(4831..4980)
                     /locus_tag="PK812_08735"
     CDS             complement(4831..4980)
                     /locus_tag="PK812_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HRJ69681.1"
                     /translation="MIPHLFAAIQRWFAYRETVRELSSLDDRELADLGITRADIESVA
                     RQAAA"
     gene            5273..6709
                     /gene="puuE"
                     /locus_tag="PK812_08740"
     CDS             5273..6709
                     /gene="puuE"
                     /locus_tag="PK812_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase PuuE"
                     /protein_id="HRJ69682.1"
                     /translation="MSIDIGPRGADYPRDMTGYGRNPPHARWPGGAKVAVQFVLNYEE
                     GGENCILHGDAASEAFLSEIVGAAPWPGQRHMNMESIYEYGARAGFWRLWRLFTDARL
                     PVTVYGVATALMRARDQVAAMKEAGWEIASHGLKWIDYRDHSEDSERAHFKLAMQIHE
                     EVCGERPLGWYTGRSSIHSTKLAMEDGGLLYSSDAYADDLPYWLRGPHGPHLIVPYTL
                     DANDMRFATPQGFNSGDQFFSYLKDSFDVLYEEGRLGAPKMMSIGLHCRLVGRPGRIA
                     ALRRFIDYIQDFDGVWCPRRVDIARHWIREHAPEGLKPSTMSPAIFIERYGDIFEHSP
                     WVAAAAHRKGLGSGGDTAAGLHARMVSALHAGTREQKLALIRAHPDLAGRLALAKQLT
                     VDSTKEQASAGLDTLSAAELARFTELNDAYKAKFGFPFIKAVKGATKDIILSAFEERL
                     PNDPAKEFDAALAEIEKIAMFRLKDRLP"
     gene            6735..>7984
                     /gene="xdhA"
                     /locus_tag="PK812_08745"
     CDS             6735..>7984
                     /gene="xdhA"
                     /locus_tag="PK812_08745"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase small subunit"
                     /protein_id="HRJ69683.1"
                     /translation="MSRDRIRYIRRGRVVDMPAGPPDRTLLDHLRLDCGATGTKEGCN
                     EGDCGACTVVLARLRGGQIVHEAVNACILLAGQVDGAEVITVEDLGSPEALHPVQQAM
                     VNHHGSQCGFCTPGIVMSLFALYQDGARPVTHQTVCDALAGNLCRCTGYRPIAAAAKD
                     ACAGAPDDAFRADEAARMARLAALDDWADVFVGTDACFFAAPASTEALARLAEAHPDA
                     VLIGGATDAGLWVTKKLQRLEKIIWLGRVRALDTVRDLPDALDLGAGVTVRKAQAHLA
                     AIDPDLGELVRRFGSEQVRASATVGGNIANGSPIGDLAPCLIALDARVTLQKGAHTRA
                     LPLERFFIAYGRQDRAPGEFVRSLSVPKLEAGQHFRCFKVSKRFDEDISAVMGAFRFT
                     LADGAVRAARIAFNNITTTPKHTPT"
BASE COUNT         1279 a         2554 c         2761 g         1390 t
ORIGIN      
        1 gtttgcttct tgccgacctg gaactgcgcg acctggtcgg ggcagaagcg gccgacgtcg
       61 ttcgggcacg ccgagaccgc tgcggcgagt tcgcccgcgt ccgggcggtg ccggcgtggc
      121 tgccagacgg tctcggcgaa gaagctttcg ggaaagtcga aggcctcggc ctgcgtgtcc
      181 tggcagagcg acaatgtcac cggtccgcat tcggccggat cggtcagcac ctgcatggcc
      241 cgggcgaagg ccgggatgat ctgctccggg cgcgtgatgc ggtcgaagta gcgcgagacc
      301 ggccggaagc agtcgttggc cgagaccgtg ccgtcgccga aatcctcgac ctgctgcagc
      361 accggatccg gccggcggcc cgcaaagacg tcgcccggca gcagcagcac cggcaggcgg
      421 ttgacatgcg ccaccgccgc cgccgtcacc atgttgagcg cgcccggacc gatggaggtg
      481 gtgcaggcca tcatgcgccg acggcgcgag gccttggcga aggcgatcgc ggcatgcgcc
      541 atcgcctgtt cattgtgcgc ccgccaggtc ggcaggacgt cgcgcgcgcc gtacagcgct
      601 tcgccgagcg ccgcgacatt gccatggccg aagatggccc agcagccggc aaacagcggc
      661 acgatctgtc cgtcgatctc ggtcttctgg gcggccatgg cgcgcaccag cgcctgggcc
      721 atggtcaggc ggatcgtctt catggacgcc tcgctgcttc ctgcggaacc gggttcagcg
      781 tgaaccgatc cgcgccgatt gtccaccgcg cgcatgatgc cgttcagccg cgcgccctag
      841 cgcttcttcg ctgcaagcgg gcggccgtag agttcgagcc ggtggccggt cagttcgtag
      901 ccgagccggc gggcgatctc gatctgcagc gcctcgatct cgggcgagga gaattcgatg
      961 acgcggcccg actgggtgtc gatcagatgg tcgtgatggg ccgttggcgc gcgttcgatg
     1021 cgcacgctgc gtccatcgaa cgtgtgctgc tcgatcaggc cgatgtcggc gaactggcgc
     1081 agcgtgcggt agaccgtgga gagcgacacc gtcggatcgc gctccatcac ccgccggtgc
     1141 agttcgcgca cgtcgggatg gtcgtgggcg gccgacagcg cttccgcgat gatcttgcgc
     1201 ggtccggtaa tgcgcaggcc gtggcggcgg caggcctcga cgaaattctc acgcatgccc
     1261 gtccgtgtct cccatcctcg cgccggggcg tggcgatccg gcgtggtcct gttcgcagtc
     1321 gcccggacct ggggcgctcg tgcattgacg gagccgggca actgcgatag tctccttatt
     1381 gcaaatcgtt tgcatttgca ataagggctg acgatcatgc ttcaggcatc cgggcagggc
     1441 tggcgcaggg cgcaggggga accgccgctt gtcgatgtgt tccgctccat cgcgctgctg
     1501 cccgatgcgt cggcctggcg caaattcctc gccttcctcg ggccgggcta tctggtcgcc
     1561 gtgggctaca tggacccggg caactgggcg acctcgctcg ccggcggcgc gcagttcggg
     1621 tacaccttgc tggttgtcgc gctggtgtcg aacatcatgg cgatcatcct gcagtcgctg
     1681 tgcgcgcggc ttgcggtggc gaccaaccgc gatctggcgc aggcctgccg cgatgcctat
     1741 ccgcgctggg tctcctggcc gctttggctg ttcgccgaac tcgcgatctg cgccaccgat
     1801 ctggccgagg tgatcggcac ggcgatcgcg ctcaatctgc tgttcggcat tccgctggag
     1861 atcggcgtgc tgatcaccgc cttcgacgtg ttcctgatcc tgtggctgca gaacaagggc
     1921 tttcgctgga tcgaggcatt catcatcacc ctgctgggtg tgatcgcggt ctgcttcgcc
     1981 atccagatcg cgctggccga tcccaactgg ggcgaggtga tccgcggctt cgcgccgacc
     2041 accgatatcg tgcgcaatcc gaacatgctg ttcatcgcgc tcggcatcat cggtgccacg
     2101 gtgatgccgc acaacctcta cctgcattcg ggcatcgtgc agacccgcgc ctatggcggc
     2161 agcctcgacg agaagcggga tgcgctgaaa tgggcgacgg tggattccac cgtggcactg
     2221 atgttcgcgc tgaccataaa cgcctcgatc ctcatcctgg cggccgcgac cttccacaag
     2281 gcgggacaga cggatgtggc cgaactgggc cgtgcgcacg agttgctggc gccgctcgtc
     2341 ggcgcgtcga tcgcgccggc gttgttcgcc gtggcgctgt tgtgctgcgg gctgaactcg
     2401 actgtcaccg cgacgatggc cggccagatc gtcatggaag gcttcatcga cattcgcctg
     2461 ccggcctggc tccggcgcct ggtgacgcgg ctcgtcgcca tcatcccggc cgtcgtcgtg
     2521 acgctgctct acggtgaagg ccagacggcg aagctcctga tcctcagcca ggtgatcctc
     2581 agcttccagc tgcccttcgc catcgttccg ctggtgatgt tcaccgcctc gcgcgaaaag
     2641 atgggcgatc tcgtcgcgcc ccgctggctc acggccatcg ccgcgctgat cgccatcacg
     2701 atcatcgcgc tcaacgtgaa actgctggtg gacgtcgcga cgggatgagc gaggtcggcg
     2761 cggcgggcct cacggctcca gcggcgggtc gccgacgccg acgacattct ggtcgaagtc
     2821 gatgttggcg ccggtcatca ggccggactc gtccgaggcg agataggcgc aggcccgcgc
     2881 cacctccttc gggtcgatca gccggccgaa gggccgggtc ctggcggcag cctccagcca
     2941 gccgtcctcg gcgccgtggt atttcttcat gatcgcgtcc tcgcccggcg tgtccatcca
     3001 gccgatgttg aggccgttga cgcggatgcg gtgcctgacc agcgcatggg cgacattacg
     3061 ggtgagggtc gcgagcgcgc ccttcgacat ggagtagggg gtcaggaacg cctgcccgcc
     3121 gttcgccgac atcgactgga tgttgatgat cgagccggcg atcttctccc gcttcatgat
     3181 cttcgccgcc tcctgcatca ggaagaaggg cgagcggatg ttcaccgcca ccatgcggtc
     3241 gaagaggtcc tcgctggtat cgaagatggt gccccggtcg gtgatgccgg cggcgttgac
     3301 gagaatgtcg atccggccga attcggcatc cgccttgcgc gtcacggcgc gggcgtcagc
     3361 ggtcttctcc aggtcggcga cgacgaacag ggcgcggcag ccggtccggg cgatgtcgcg
     3421 ggcgaccgcc tcgcccttgt ccttctgtcg gccgcagagg acgaggccct tgagaccgcg
     3481 cgcggcgaat tcccggccga tcgcctcgcc cagcccctgc gtgcctccgg tgacgaccgc
     3541 gaccttgccg tcgagacggg aatgggggct gggcagggtc atcgtgcaac tccgggcggc
     3601 tgaaaggatg gcgattcgaa gtcgacttca gagaacacgg tttcgcaagc cgaaatccgg
     3661 cgttcagggg ccgagcatga acgtttcttc gagactttcg gggacgtcgc ggcgcggcgg
     3721 gcggccttgc gcgcggcctg cgattctggt gtctgttgcc ggcacacagg cgggaggagc
     3781 gcgcgatgct gaaggtcgga ttgctgggag cgggccgcat cggccgcatt cacggcggca
     3841 atgttgcggt gcacccgcgc gccagactcg tggcggtagc ggacacccat gccccggcgg
     3901 cggaagccct tgcccaggaa accggtgcga aaaccgcctc gatcgaggcg atcctgaagg
     3961 acaagaccat cggcgcggtg gtgatctgca ccccgacgga catgcatgcg gatctgatcg
     4021 aggcggcggc gaaggcgaag aaggcggtgt tctgcgagaa gccggtcgat ctgaattcgg
     4081 atcgcatccg cgcctgcctc aaggtcgtgg ccagggcgaa gacggcgctg atgatcggct
     4141 tcaaccgccg ctttgatccg aatttttccg cgctgaaaca aaggcttgcg ggaggcgaag
     4201 tcggcaaggt cgaactcgtg accatcctgt cgcgcgaccc tggcccgccg ccggcggagt
     4261 atgtcgcgcg gtccggcggc ctgtttcgcg acatgatgat ccacgacctc gacatggcgc
     4321 gccatctgct cgacgaggag ccggtggagg tgcacgcggt gggctcgtcg ctggtcgaca
     4381 aggccatcgg caaggcgggc gacgtggaca ccgcggccgt gctcttgaag acgaaatcag
     4441 ggaagatcgc gcagatttcc tgctcgcgcc gggcgaccta cggctatgac cagcggatcg
     4501 aggtgcatgg atcgaaaggc ctgattcggg ccggaaacgt acacaacacg acggttgaag
     4561 tggcgacggg ggccggtttt cgggccgatc cggtgcagaa cttcttcctg gagcgctatg
     4621 ctgctgccta ccggaacgag cttgagagct tcatcgcggc ggtcgagaag ggcgaggcgc
     4681 cgtccccctc gggcgaagac gggctcaagg cgcaggtgct ggctgatgcc gccacagagg
     4741 cctgccagac cggaaagacc gtgcgcctgt gagaaaacgc gagacgggga ggcgcagcct
     4801 ccccgtcctg gcgtaaattt cgcaatgaaa tcaggctgct gcctggcggg cgacgctctc
     4861 gatgtcggcg cgggtgatgc cgaggtcggc cagctcgcgg tcgtcgaggc tcgacagctc
     4921 gcggacggtt tcgcgatagg caaaccagcg ctgaatagcg gcgaacaggt gcgggatcat
     4981 cgtttactcc atcagtcggt gcggaaaggg aagtggcggc agctcgccgc gttgcaaaaa
     5041 tctatatatg cactgcagca attccacaaa cgcatatatc tcatgggagg tatgcgaact
     5101 tgccggcgct cgcttacgaa ctgttaggaa ttggagcgga gacgcggggc atttcgcggc
     5161 cgagcggctg ctctgcggaa attccaagga actgtttgcg cgcaattttt gaagatcgcg
     5221 gcttgagctt gccgcctgct tccgcgatcc tgccgcggga ggcagcaacg acatgagcat
     5281 cgacatcgga ccgcgcggcg cggactaccc ccgggacatg accggatacg ggcgcaatcc
     5341 gccgcacgcg cgctggccgg gtggcgcgaa ggtcgctgtc cagttcgtgc tgaactacga
     5401 ggagggcggc gagaactgca tcctgcatgg agatgcggcc tccgaagcct tcctgtcgga
     5461 gatcgtcggc gccgcgccct ggcccggcca gcgccacatg aacatggagt cgatctacga
     5521 gtacggtgcc cgcgccggtt tctggcggct ctggcgcctg ttcacggatg cgcgccttcc
     5581 cgtcacggtc tacggcgtcg caacggcgct gatgcgcgcg cgcgaccagg tggccgccat
     5641 gaaggaagcg gggtgggaga tcgcctcgca cgggctcaag tggatcgact atcgcgacca
     5701 ttcggaggat tccgagcggg cgcatttcaa gctggcgatg cagatccacg aggaggtctg
     5761 cggcgagcgg ccgctcggct ggtataccgg ccgcagttcg atccattcga ccaaactggc
     5821 gatggaagac ggcgggctgc tctattcctc cgatgcctat gccgacgacc tgccgtactg
     5881 gctgcggggg ccgcacggcc cgcatctgat cgtgccctac acgcttgacg ccaatgacat
     5941 gcggttcgcg acgccgcagg ggttcaattc cggagaccag ttcttcagct atctgaagga
     6001 ttccttcgac gtcctgtatg aagaggggcg tctcggcgcg ccgaagatga tgtcgattgg
     6061 cctgcactgc cgtctggtcg ggcgccccgg tcgcatcgcg gcgctgaggc gattcatcga
     6121 ctacatccag gatttcgacg gcgtctggtg tccccggcgt gtcgacatcg cgcgccactg
     6181 gatccgcgaa cacgcgccgg agggcctgaa gccgtcgacc atgtcgccgg ccatattcat
     6241 cgagcgctat ggcgacattt tcgagcattc gccctgggtt gcggcagcgg cgcaccgcaa
     6301 ggggctgggg tccggcgggg atacagcggc aggcctgcac gcacgcatgg tctcggcgct
     6361 gcatgccgga acgcgcgaac agaagttggc gctcatccgc gcccatcccg atcttgccgg
     6421 ccgtctggcg ctcgccaaac agttgaccgt cgactcgacg aaggagcagg cgtcggcagg
     6481 cctcgacacg ctgtcagctg cggaactcgc gaggttcacg gagctgaacg acgcctacaa
     6541 ggcgaaattc ggcttcccgt tcatcaaggc ggtgaagggc gcgaccaagg acatcatcct
     6601 gtccgccttc gaggaacgcc tgcccaatga tccggcgaaa gaattcgatg cggctctggc
     6661 cgaaatcgaa aagatcgcca tgttccgtct gaaggatcgc ctgccgtgac gggcgggcga
     6721 ggggagggat gcgcgtgagc agggatcgaa tccgctacat ccggcgcggc cgggtggtgg
     6781 acatgccggc ggggccgccg gaccggacgc tgctcgacca cctgcgcctc gactgcggcg
     6841 caaccggcac gaaggaaggt tgcaatgagg gcgactgcgg cgcctgcacg gtggtgctcg
     6901 cgcgcctgcg tggcgggcag atcgtgcacg aggcggtcaa cgcctgcatc ctgctggccg
     6961 ggcaggtcga cggcgcggag gtgatcaccg tcgaggatct gggatcgccg gaggcgctgc
     7021 atccggtgca gcaggcgatg gtgaaccatc atggttcgca atgcggcttc tgcacgccgg
     7081 gcatcgtcat gagcctgttc gcgctctatc aggacggcgc gcggccggtg acgcaccaga
     7141 cggtgtgcga tgcgctggcc ggcaacctct gccgctgcac cggctatcgt cctatcgctg
     7201 ccgcggcgaa ggacgcctgc gccggtgcgc cggacgacgc cttccgggcg gacgaggcgg
     7261 cgcgcatggc ccggctcgcc gcgctggacg actgggcaga tgtctttgtc ggcacggacg
     7321 cgtgcttctt tgccgcgccg gcctcgaccg aggccttggc acggttggct gaagcccatc
     7381 ccgacgcggt gctgatcggt ggcgcgacgg acgccgggct ctgggtgaca aaaaagttgc
     7441 aacggctgga gaaaatcatc tggctcggcc gtgttcgcgc tctggacacg gtgcgcgacc
     7501 tgcccgatgc gctcgacctc ggcgccggcg tgacggtccg caaggcgcag gcccatctcg
     7561 cggcgatcga ccccgacctc ggcgagctgg tgcgtcgctt cggttccgaa caggtgcgcg
     7621 cctccgcgac ggtcggcggc aacatcgcca acggctcgcc cattggcgat ctggcgcctt
     7681 gcctgatcgc gctcgatgcg cgcgtgacct tgcagaaggg cgcgcacacg cgtgcgctgc
     7741 cgctggagcg cttcttcatc gcgtatggcc ggcaggatcg cgcgccggga gaattcgttc
     7801 gctccctgtc cgtgcccaag cttgaggcgg gccagcattt ccgctgcttc aaggtctcca
     7861 agcggttcga cgaggacatc tcggcggtga tgggcgcgtt ccgcttcacg ctggccgatg
     7921 gcgcggtgcg cgccgcgcgg atcgccttta acaacataac aacaacacca aaacacacac
     7981 caac
//
LOCUS       DAOMXN010000112         7931 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_4772_length_7931_cov_12.433977, whole genome shotgun sequence.
ACCESSION   DAOMXN010000112 DAOMXN010000000
VERSION     DAOMXN010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 7931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7931
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4772_length_7931_cov_12.433977"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..407)
                     /locus_tag="PK812_08750"
     CDS             complement(18..407)
                     /locus_tag="PK812_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020372397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HRJ69684.1"
                     /translation="MDADQKVDPWPTEIRVSADRRALTVGFEGGDVFTLAAEFLRVMS
                     PSAEVQGHTPEQKKTVPGKRDVVILKVEPVGNYAVRIVFDDLHDSGIYGWRLLRRYGR
                     EQTALWQAYLDDLAARGMTRDPARRAP"
     gene            535..1554
                     /gene="moaA"
                     /locus_tag="PK812_08755"
     CDS             535..1554
                     /gene="moaA"
                     /locus_tag="PK812_08755"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002964078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HRJ69685.1"
                     /translation="MSLSTEPTLPQLTDPFGRDVTYLRVSVTDRCDFRCAYCMSEHMT
                     FLPKQDLLSLEELDRLCSLFVRRGVRKLRITGGEPLVRRDIMRFFRAMARHLGSGLDE
                     LTVTTNGSQLRRFASELKDCGVERINVSLDTLNADKFREITRWGDLAKVLDGIRAARD
                     AGLRVKINTVALKGFNDAESVELIRWAHGEGMDITFIEVMPLGEIESARVDQYLPIST
                     LRAQLADHFTFEGTDYRTGGPARYARIRETGGLIGFITPLTHNFCESCNRVRLTCTGT
                     LYMCLGQEDAADLRKVLRGSEGDETVLAAIDAAIARKPKGHDFVIDRRTRLPAVGRHM
                     SVTGG"
     gene            1600..2520
                     /locus_tag="PK812_08760"
     CDS             1600..2520
                     /locus_tag="PK812_08760"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRJ69686.1"
                     /translation="MSGRLSPNLRGMLALTASSGIFSISDTLMKLSTGHWPVSQAMTF
                     RGVVAIGLSLIAVAWTGNLSGLSALRNPLVLLRTVCEGALALMFIYALSLMPLADLTS
                     ILMLSPLVITAIVALFYGEQVGWRRWSAIMAGFVGMLMVIQPGQEARSDTYQFAVLLG
                     LGSVAGVAARDLTTLRLKADVPSVVVMLGSAIGGCLAGLVLAPFETWRPWTHTPAIAL
                     IGAALLVSAANFLLIIASRGVDLSAVAPFRYSAVVFAILLGVSVFGDVPNQMALAGMG
                     VIVASGVYLMHRERVRRQARAAAKSTADES"
     gene            2648..3265
                     /locus_tag="PK812_08765"
     CDS             2648..3265
                     /locus_tag="PK812_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HRJ69687.1"
                     /translation="MDGLLSLSKGIDWLNEKIGKSAAWLILVAVLISAVNAIIRKVFN
                     TSSNAWLEAQWLLFGAVFLLCAPWALLHNEHIRIDIVNSKLSQKWKRLIEFVGHGLFV
                     IPICLVMIATAWPFFMTSWRVNEQSTNAGGLPQYPAKFLVLLGFVLLLLQGISEFIKR
                     MAVSKGMIEDSLHGGGHHAAAEAEAQRLLEVAKAEAEKLASEAKK"
     gene            3293..5299
                     /locus_tag="PK812_08770"
     CDS             3293..5299
                     /locus_tag="PK812_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRJ69688.1"
                     /translation="MAEFIAHNIAPIMFVALVVFLLLGYPVAFALGACGLVFFVIGVE
                     LAPFSNGVISLSWPLLQSLPDRLYGGVMANDVLLAIPFFTFMGLVLERSGMAEDLLDT
                     IGQLFGTMRGGLAYAVIFVGALLAATTGVVAASVISMGLISLPIMLRYGYDRRLASGV
                     IAASGTLAQIIPPSLVLIVLADQLAADVGSMYEGAFVPGLVLSALYAGYVFIVTMLYP
                     SAAPGLPVDAVGFKEAPTAADRLARVNAAILIAALLAGLVWFYPSLRTGILDGLAGDW
                     KAKISGVLLLGFVAYLFYGLFNLVGALKSGGDERGLLSLLTLTLFSAVVGYLFMSRTK
                     VSAGADFVILSMSIAVAVAFAVAVVSWLCDKVLGFRFLSKLAQQVTFVMVPPLALIFL
                     VLGTIFIGVATPTEGGAMGAAGALLLAAFKRYLDKDPSRFNFTIIRQATESTAKLSAF
                     VIFILVGARIFSLTFYGVNGHIWVEEHLKSLPGGFYGFLILINIAVFLLAFFLDFFEL
                     AFIVIPLIRPAAEAIFITDPTALEIAKSFNLTSESEIKKAMIVWFGIMMGVNMQTSFM
                     HPPFGFALFYLRSVAPKEPYKDKVTGKMTDPVTTGQIYWGAVPFVVIQLIMVTLVMLT
                     PSMVISKKPEKPAVDPAKAMEQLQNLSPRGLDLPALDLGPPKIQ"
     gene            complement(5542..6627)
                     /locus_tag="PK812_08775"
     CDS             complement(5542..6627)
                     /locus_tag="PK812_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRJ69689.1"
                     /translation="MKRRQFLTAGAGLAATAVAAPAIAQSAPEIKWRLTSSFPKSLDT
                     IFGAAEVFSKYVAEATDNKFQVQVFAAGEIVPGLQAADAVTAGTVEMCHTASYYYFGK
                     DPTFAFGTAVPFGLNARQQNAWWMHGGGEKLMNEFYAKHKIHGLLAGNTGCQMGGWFR
                     KEIKTVDDMKGLKMRIGGFAGQVMAKLGVVPQQIAGGDIYPALEKGTIDGAEWVGPYD
                     DQKLGFNKVAPFFYYPGWWEGGAMLHNFVNIDKWNALPKGYKAIVEAASHVADQDMQA
                     KYDARNPAALIDLVRNGGAQLRPYPQEVLAACFKAANEVYAETNAKNADFKKVHDNIM
                     AFRNDAYLWWQVAEYTYDNFMIRARQG"
     gene            complement(6791..7906)
                     /locus_tag="PK812_08780"
     CDS             complement(6791..7906)
                     /locus_tag="PK812_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HRJ69690.1"
                     /translation="MSTKDAVLAALGSVPGPDGKTPLTQSGALSDLVVANGKAYFSIA
                     VDPARAGDSEPMRKAAETAAKSVSGIEAAIVTLTAERKAAAQPAKPAAPGAGPAQPQG
                     AKLAVQGVKHIIAVASGKGGVGKSTTACNLALAFTRLGLKVGVLDADIYGPSMPRLFA
                     LKGRPTLAGGRTLNPLEGYGVKVMSIGFLVEEDTAMIWRGPMVISAIQQMLREVAWGD
                     LDVLVVDMPPGTGDAQLTMAQNVPLSGAVIVSTPQDLALIDARRGIAMFRKVEVPILG
                     VVENMSTFICPDCGGRHDIFGHGGARDEASRIGAPFLGEVPLAMPIRERSDAGRPIVI
                     SEPDGPHAQCYLAIARKVWQGLDAGDARRAAPRIVFD"
BASE COUNT         1270 a         2593 c         2558 g         1510 t
ORIGIN      
        1 ctgagccgcg ccttctgtca gggcgccctg cgcgccggat cgcgcgtcat ccccctggcg
       61 gcaaggtcgt cgagataggc ctgccacagg gcagtctgct cccgcccgta gcgccgtagc
      121 agccgccagc cgtagatgcc gctgtcgtgc agatcatcga acacgatgcg cacggcgtag
      181 ttgccgaccg gttcgacctt caggatgacg acatcgcgct tgcccgggac ggtcttcttc
      241 tgctccgggg tgtgcccctg cacctcggcg ctggggctca tcacgcgcag gaattcggcc
      301 gcaagcgtga agacgtcccc gccctcgaaa ccgacggtca gcgcgcgccg gtcggccgaa
      361 acgcggattt ccgtcggcca gggatcgacc ttttgatctg catccatcga catgcctccg
      421 tactcccggt tatcaagaga ccgcatgggg gtgatcggca atgctcttgc cgaatgcgcg
      481 gtcccggcac aagttagcgt atcaaggtcc aaagcacaca gccgcgtgac aaccatgtcc
      541 ttatcgaccg aacccaccct gccgcagctg accgatccgt tcgggcgcga tgtgacctat
      601 ctgcgggtct cggtcaccga ccgctgcgac ttccgttgcg cctactgcat gtcggaacat
      661 atgacgttcc tgcccaagca ggatctcctg agcctggagg aactggaccg gctctgctcg
      721 ctgttcgtca ggcgcggcgt gcgcaaactg cgcatcaccg ggggcgagcc gctggtgcgg
      781 cgcgacatca tgcgcttctt ccgcgcgatg gcgcggcatc tcggctccgg gctggacgaa
      841 ctgacggtca ccaccaacgg ctcgcagctg cgccgcttcg catcggaact gaaggattgc
      901 ggcgtcgagc gcatcaacgt ctcgctggat acgctcaacg cggacaagtt ccgcgagatc
      961 acgcgctggg gcgatctcgc caaggtgctg gacggcatcc gggcggcgcg cgacgccggg
     1021 ctgagggtca agatcaacac ggtggcgctg aaaggcttca acgacgcgga gagcgtcgaa
     1081 ctcattcgct gggcgcatgg cgagggcatg gacatcacct ttatcgaggt gatgccgctg
     1141 ggcgagatcg agagcgcgcg ggtggaccag tacctgccca tctcgacgtt gcgggcccag
     1201 ctcgccgacc atttcacctt cgagggtacg gactatcgca ccggcggccc cgcgcgctat
     1261 gcccggatcc gcgaaaccgg cggactgatc ggcttcatca cgccgttgac gcacaacttc
     1321 tgcgaaagct gcaaccgggt gcgcctgacc tgcaccggaa cgctctacat gtgcctcggc
     1381 caggaagatg cagccgacct gcgcaaggtc ctgcgcggct cggaaggtga cgagacggtg
     1441 ctggcggcca tcgacgccgc catcgcgcgc aagccaaagg gccatgactt cgtcatcgac
     1501 cggcgcacgc gtctgcccgc cgtcggccgt cacatgtccg tcaccggcgg ttgatcccgc
     1561 tcctgccttt ggccgccttc ggacggattg cgctgtgtcg tgagtggacg tctttcccct
     1621 aacctgcgcg gcatgctggc cctcacggcc tcttccggca ttttctcgat cagcgacacg
     1681 ctgatgaagc tctccaccgg acattggccg gtatcgcagg cgatgacgtt tcgcggcgtg
     1741 gtggcgatcg gtctgtcgct catcgccgtg gcctggacgg gaaacctctc cgggctgtcg
     1801 gccctgcgca atccgctggt gctgctgcgc accgtgtgcg aaggcgcgct ggcgctgatg
     1861 ttcatctatg cgctcagcct gatgccgctg gccgacctga cctcgatcct catgctgtcg
     1921 ccgctggtca tcacggcgat cgtcgccctg ttttatggcg agcaggtggg ctggcggcgc
     1981 tggagcgcca tcatggcggg cttcgtcggc atgctcatgg tcatccagcc aggccaggag
     2041 gcgcgctcgg acacctacca gttcgcggtc ctgctgggcc tcgggtcggt cgcaggcgtg
     2101 gccgctcgcg acctgacgac gctgcgcctc aaggccgatg tgccaagcgt cgtggtgatg
     2161 ctcggctccg ccatcggcgg ctgccttgcg ggactggtgc tggcgccgtt cgagacgtgg
     2221 cggccatgga cgcatacgcc ggccatcgcg ctgatcggcg ccgcgcttct ggtcagcgcg
     2281 gcgaacttcc tgctgatcat cgccagtcgc ggcgtggacc tctcggcggt cgcaccgttc
     2341 cgctacagcg ccgtggtgtt cgcgatcctg cttggcgtgt cggttttcgg cgacgtgccg
     2401 aaccagatgg ccctggccgg catgggtgtg atcgtcgcca gcggcgtcta tctgatgcac
     2461 cgcgagcgcg tgcgccggca ggcgcgcgcc gctgcaaaat cgactgccga cgaaagttga
     2521 agaaacgcca ttggcgccag ttgtcggcac cgcctcgggc ctgtaaggaa agcccaaagc
     2581 gcggcgtcgg aaggcacgtt tccagcgatg accgcacgca acgtgaccgc actacgggga
     2641 gctgtgcatg gatgggctgt tgagtctttc gaagggcatc gactggctga acgaaaagat
     2701 cggaaagagc gcggcatggc tcattctggt tgccgtcctc atttcggcgg tcaacgccat
     2761 catccgcaag gtgttcaaca cctcgtcgaa tgcctggctt gaggcgcagt ggctgctgtt
     2821 cggcgcggtg ttcctgctgt gcgcgccctg ggccctgctg cacaacgagc atatccgcat
     2881 cgacatcgtc aattcgaagc tctcgcagaa gtggaagcgg ctcatcgagt tcgtcggcca
     2941 cggcctgttc gtcattccga tctgccttgt catgatcgcc acggcctggc cattcttcat
     3001 gacgtcctgg cgcgtcaatg aacagtcgac caatgccggc ggcctgccgc agtatcccgc
     3061 caaattcctg gtgctgctgg ggttcgtgct gctactgctt cagggtattt cggaattcat
     3121 caagcgcatg gccgtgtcca agggcatgat cgaggacagt ctgcacggcg gcggccatca
     3181 tgccgcggcc gaggccgagg cccagcgcct gctcgaagtc gccaaggcgg aagcggaaaa
     3241 gctcgcttcc gaagccaaga aataagacaa gagcggttcg gggatcatca tcatggctga
     3301 gttcatcgcc cacaacatcg cgccgatcat gttcgtggcg ctcgttgtct tccttctcct
     3361 gggctatccc gttgccttcg cgctcggcgc ctgcggcctc gtgttcttcg tcatcggcgt
     3421 cgaactggcg ccgttctcga acggtgtgat ctcgctgagc tggccgctgc ttcagtcgct
     3481 tccggatcgg ctctacggcg gcgtgatggc caacgacgtg ctgctggcga tcccgttctt
     3541 cacattcatg gggctggtgc tggagcgctc gggcatggcg gaagacctgc tcgacaccat
     3601 cggccagctg ttcggcacga tgcgcggcgg tcttgcctat gcggtgatct tcgtcggtgc
     3661 gctgctggca gccaccaccg gcgtcgtggc cgcatcggtg atctcaatgg gcctcatctc
     3721 cctgccgatc atgctgcgct acggctatga tcgtcgtctg gcctcgggcg tcatcgcggc
     3781 ttccggaacg ctggcgcaga tcattccgcc gtcgctggtg ctgatcgtgc tggccgacca
     3841 gctcgcggcg gacgtcggct cgatgtacga gggcgccttc gtgcccggtc tggtgctgtc
     3901 cgcgctctat gccggctatg tcttcatcgt cacgatgctg tatccgagcg cggcgcccgg
     3961 tctgccggtg gatgccgtcg gtttcaagga ggcgcccacc gccgccgacc gcctggctcg
     4021 cgtcaacgcc gccatcctga tcgccgcgct ccttgcgggg ctggtctggt tctatccgtc
     4081 gctgcgcacc gggatccttg atgggctggc cggggactgg aaggcgaaaa tcagcggcgt
     4141 gctgctgctc ggtttcgtgg cctacctgtt ctatggcctg ttcaatctgg tcggcgcgct
     4201 gaaatccggc ggcgacgaac gcggtcttct ctcgctgctg acgctgacgc tcttctccgc
     4261 cgtggttggc tacctcttca tgagccgcac gaaagtcagc gccggggccg atttcgtgat
     4321 cctgagcatg tcgatcgccg tcgcggtggc gtttgccgtg gcggtggtga gctggctgtg
     4381 cgataaggtc ctcggattcc gcttcctgtc gaagctggcc cagcaggtga ccttcgtgat
     4441 ggttcctccg ctggcgctga tcttcctcgt gctcggcacc atcttcatcg gcgtcgcgac
     4501 gccgaccgag ggcggcgcga tgggcgcggc cggcgcgctg ctgctggccg ccttcaagcg
     4561 ctacctcgac aaggacccgt cacgcttcaa cttcaccatc atcaggcagg ccacggaatc
     4621 gacggcgaag ctgtcggcct tcgtgatctt catcctggtc ggcgcgcgca tcttctcgct
     4681 gaccttctat ggcgtgaacg gccatatctg ggtcgaggag catctgaagt ccctgcccgg
     4741 cggcttctac ggcttcctga tcctgatcaa catcgcggtg ttcctgctgg ccttcttcct
     4801 cgacttcttc gagctggcct tcatcgtcat cccgctcatc cgcccggcgg ccgaggcgat
     4861 tttcatcacc gatccgacgg ccctggaaat cgcgaagagc ttcaacctga cgtcggaaag
     4921 cgaaatcaaa aaggcgatga tcgtctggtt cgggatcatg atgggcgtca acatgcagac
     4981 gagcttcatg catccgccct tcggcttcgc gctgttctac ctgcgctccg tcgcgccgaa
     5041 ggagccctac aaggacaagg tcacgggcaa gatgaccgat cccgtgacca ccggccagat
     5101 ctactggggc gcggtgccgt tcgtggtgat ccagctcatc atggtcaccc tggtcatgct
     5161 gacgccctcg atggtgattt cgaagaagcc cgaaaagccg gccgtcgacc cggccaaggc
     5221 catggagcag ttgcagaacc tctcgcccag gggcctggac ctgcccgcgc tcgatctcgg
     5281 cccgccgaag atccagtagt ccgcttccag caccggacat gcagaagggg cgtcccgtcg
     5341 ggacgcccct ttctctatgg acaatggcaa gttgcaggcc tgaagtccgg cagtcggccc
     5401 gccccctgcc ccgcaagagg caggcagcct ggaaaagccg tgggtggcgc agcggtcaat
     5461 gccgccgcta gcctgcggca ggccgcaaaa ggaaacggcg gcccgaaggc cgccgctcgc
     5521 tcgtcgacgt tgatccgggg atcagccctg gcgggcgcgg atcatgaagt tgtcgtaggt
     5581 gtattccgcg acctgccacc agaggtaggc gtcgttgcgg aaggccatga tgttgtcgtg
     5641 gaccttcttg aagtccgcgt tcttggcgtt ggtttcggca tagacctcat tcgcggcctt
     5701 gaagcaggcg gcgagcacct cctgcggata cggacgcagc tgcgcgccgc cgttgcggac
     5761 gagatcgatc agcgcggccg ggttgcgcgc gtcgtacttg gcctgcatgt cctggtcggc
     5821 aacgtgagac gcggcctcga cgatggcctt gtagcccttc ggcagcgcgt tccacttgtc
     5881 gatgttgacg aagttgtgca gcatcgcgcc gccttcccac caaccgggat agtagaagaa
     5941 cggggcgacc ttgttgaagc ccagcttctg gtcgtcgtag ggaccgaccc actccgcgcc
     6001 gtcgatggtg cccttttcaa gcgccgggta gatgtcgccg ccggcgatct gctgcggcac
     6061 gacgccgagc ttcgccatga cctgaccggc gaagccgccg atgcgcatct tcaggccctt
     6121 catgtcgtcg acggtcttga tttccttgcg gaaccagccg cccatctgac agccggtgtt
     6181 gccggcgagc agaccatgaa tcttgtgctt ggcgtagaac tcgttcatca gcttttcgcc
     6241 gccgccgtgc atccaccagg cgttctgctg acgggcgttc aggccgaacg gaaccgcggt
     6301 gccgaaggca aaggtcgggt ccttgccgaa atagtaatac gaggcggtgt ggcacatctc
     6361 gacggtgccg gcggtaaccg cgtcggcagc ctgcaggccg gggacgattt cgccggccgc
     6421 gaagacctgg acctggaact tgttgtcggt ggcctcggcc acgtacttgg agaacacttc
     6481 ggccgcgccg aagatggtgt cgagcgactt cgggaagctc gaggtcaggc gccatttgat
     6541 ttcgggggcc gactgggcaa tcgccggggc ggcaacggcc gtagcggcaa ggccagcgcc
     6601 cgcggtgaga aactgacgac gcttcattat tatcctccgg aagctcggtt gggttttagg
     6661 gcgggcggtt tacccgcctt gcgcccggct agataaagcg cagaaacgaa aaaagaagca
     6721 atccccccgc tttgaaattg gccgagaatg gaacgctttc cggccgaatc ccgcacattg
     6781 ggaaagccag ctagtcgaag acgatgcgcg gcgccgcgcg gcgcgcatca ccggcatcga
     6841 ggccctgcca aaccttgcgg gcgatcgcca gataacactg cgcatggggg ccgtccggct
     6901 cggagatgac gatcggtcga ccggcatccg aacgctcccg tatgggcatg gcgaggggca
     6961 cttcaccaag gaatggcgca ccgatccggg acgcctcgtc gcgcgcgccg ccgtgcccaa
     7021 agatgtcgtg ccggccgccg caatccgggc agatgaaggt cgacatgttc tccaccacgc
     7081 cgaggatcgg cacctcgacc ttgcggaaca tggcaatgcc gcgccgcgcg tcgatgagcg
     7141 ccagatcctg gggcgtcgag acgatgaccg cgccggacag cggcacgttc tgcgccatgg
     7201 tgagctgcgc gtcgccagtt cccggcggca tgtcgacgac cagcacgtcg aggtcgcccc
     7261 aggcaacctc gcgcagcatc tgctggatgg cggagatcac catcggcccc cgccagatca
     7321 tcgccgtatc ctcctcgacg agaaagccga tggacatgac cttgacgccg tagccttcga
     7381 gcggattcag cgtccgcccg ccggccaggg tgggacggcc cttgagcgcg aacagccgag
     7441 gcatcgacgg gccatagatg tccgcatcca gcacgccgac cttcaggcca aggcgcgtga
     7501 aggcgagcgc cagattgcag gcggtggtcg acttgcccac cccgcccttg ccgctcgcca
     7561 cggcgatgat gtgcttgacg ccctgaaccg ccagtttggc cccctggggc tgcgccgggc
     7621 cggcgccggg agcggcaggc tttgcaggct gcgccgccgc cttgcgctcg gccgtcagcg
     7681 tgacgatggc cgcctcgatg ccggaaacgg atttcgccgc cgtttccgcc gccttgcgca
     7741 tgggctcgct gtcgccggcc cgcgccggat cgactgcgat ggaaaaatag gccttgccat
     7801 tggcgacgac cagatcggac agcgcgccgg actgcgtcag cggtgtcttc ccgtccggcc
     7861 ccggtaccga gccaagggcg gcgagaactg cgtctttcgt gctcatgctg gggaaacgat
     7921 cctgaagagt t
//
LOCUS       DAOMXN010000113         7911 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_4790_length_7911_cov_10.853488, whole genome shotgun sequence.
ACCESSION   DAOMXN010000113 DAOMXN010000000
VERSION     DAOMXN010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 7911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7911
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4790_length_7911_cov_10.853488"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="PK812_08785"
     CDS             <1..817
                     /locus_tag="PK812_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69691.1"
                     /translation="LWSSKFRQATRLLHLLQRPAQGLPPGVLALQAIYSGTNRPVSTP
                     VIWRIYVVHGNEPTLTATSAELAPTLPLPPGEYMVHLSQGLAAVTRRIVMTAAPLSER
                     LTLNAGGLVLRAKLAEAPVPASRQAISVFIPAPNDSEGKLVTNSLKAGELLRLPEGTY
                     HVVSTYAGSNSVVRADIQVQSGRVTEATMNHKAATMTLKLVRQVGGVAVANTSWTVQT
                     PGGDLIREALGAFPTMELAEGEYEVIARNDGREFKERMKVVSGINRDHEVVMR"
     gene            874..1110
                     /locus_tag="PK812_08790"
     CDS             874..1110
                     /locus_tag="PK812_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HRJ69692.1"
                     /translation="MQTFFMQIKCKLGQTYKVATDLAEAEIASEIYSVAGNYDILAKF
                     YVDDDVDIGHFVAQKVQTLPGIQDTLTLITFKAF"
     gene            1197..2363
                     /locus_tag="PK812_08795"
     CDS             1197..2363
                     /locus_tag="PK812_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69693.1"
                     /translation="MMRASVPAILILLGASIVAAEAQPAQGRWPMAVGERYCAGEPTH
                     PKGGLNWRLSCAAACPAGSALVTGSCRVIASDNQGIALEGAGPSINGWSCDYVAPHHD
                     VSRSGALSRRVRVKAEAHCRETAAAPPGRWSVPAEGVPIVIARSGETISEKIRFSVRL
                     CNTEGPAPVNVHLLLRDPNQPGIPERPAAMPVPPGHCLQVDQPVAVFVQNGATQQTGL
                     AGTYQLYKDGTFKPRPEIASAIDLARNARDEGAIARRAVKATAPELVRARCERLDPAP
                     KGIWGRCAIPEISELGNYRICFAPNYAPPANTEPNHPGALLPMVVNPKLVRLPLPADY
                     SAGTPGARDVHNPIQANACRDYLNVREVNVLMLESTIFPPQNVDSVSFTLQRLG"
     gene            complement(2487..4466)
                     /gene="thrS"
                     /locus_tag="PK812_08800"
     CDS             complement(2487..4466)
                     /gene="thrS"
                     /locus_tag="PK812_08800"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HRJ69694.1"
                     /translation="MISLTFPDGAVRQFPVNISGLDIAKGISPSLAKRTVAMAIDGVV
                     CDLADPIARDAKIEFLNREDPRALELIRHDCAHVLAEAVQALFPGTQVTIGPVIENGF
                     YYDFHRNEPFTPEDFAAIEKKMREIVAKDAPFTKEEWSRDQAKAFFREKGEHFKVELV
                     DAIPDDQTLKMYKQGEWLDLCRGPHMTSVGKIGTAFKLMKVAGAYWRGDSSKPMLTRI
                     YGTAFAKQDELDAYLKQLEEAEKRDHRRLGREMDLFHFQEEGPGVVFWHPKGWKMFQE
                     LVAYMRRRLAADYDEVNAPQLLDKSLWETSGHWGWYSDNMFGSVAASAIIRPQDADAD
                     RRIFALKPMNCPGHVQLFKHGLKSYRDLPMRLAEFGVVHRYEPSGALHGLMRVRGFTQ
                     DDAHIFCTDEQMEAECLKINELMLSVYADFGFDELVVKLSTRPEKRVGSDALWDRAES
                     VMMRVLEKIAAQSGGKIKTGINPGEGAFYGPKFEYTLRDAIGRDWQCGTTQVDFNLPE
                     RFGAFYIDAAGEKTVPVMIHRAICGSMERFLGILIESHGGHMPLWLAPEQISVCTITQ
                     DADDYAHAVVGRALREGLRARADLRNEKIGYKVREHSLAKTPVIFAVGKREAEEGTVA
                     IRRLGSQAQSVMKLDEALAMLADEATPPDVKRARG"
     gene            4705..5919
                     /locus_tag="PK812_08805"
     CDS             4705..5919
                     /locus_tag="PK812_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69695.1"
                     /translation="MRRNSVFQWRNFRRIGLGTVLLAALMAFASAQPAASMTFTLGRS
                     GPLPCQRDCAEFIVADGEIGPDSAAEFLALWSRLPRKDLPLMLNSPGGRLDGAMALGR
                     ALRHLNVTVTVARARRLGTETPGVAQYAARLDAGICHSACVYTLAGASRRNMAPGSEI
                     GVHQFYLARASDTKRRPKQQYSDADFGRLQSTVAALAGYLVEMQVDLRLLTLAAETEP
                     SRIRVLSSGEVAELLPAAVMVERAAAPPLSAASLHPSLSTTAPPSVHQTPGWPLVMRE
                     GRPWLVLQAESQSRRFGVISNEVAIGCRSEGADFVATFREILPPGRPASEGSARIATG
                     SDGGTLAHKPGADHLRGTISQESTMTAAQSGTLDVDVTTVATANYPMRISFAGKGLMD
                     GIDRLERACTAR"
     gene            6212..6994
                     /locus_tag="PK812_08810"
     CDS             6212..6994
                     /locus_tag="PK812_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69696.1"
                     /translation="MKLKKLGLAAAIALAMHTAPVTAQADKALTDSAASWVKVFADPK
                     FIELGMKMFDPKMMTDFMTLMANPAMMEAMMKMMSPELMTAFMKVAMDPKMIEAMMKM
                     ADPKLMEGFMKMAFDPKMMEAMMKMADPKIMTSFMTMMADPKWMEAMMKMMSPEVMTA
                     FMKMALDPKMMEAMMKMADPKIIESFVRVMFDPKMIESMMKMADPQMMQPFMAAMTDP
                     KMMDAMMKMMDPKLMMQMMTAMATMMTSWANTAPGTAAPQKR"
     gene            7069..7782
                     /locus_tag="PK812_08815"
     CDS             7069..7782
                     /locus_tag="PK812_08815"
                     /inference="COORDINATES: protein motif:HMM:NF015581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HRJ69697.1"
                     /translation="MVHRFAQAVGLSLTLLAGAATAQTPAPTPQPNTLAPAGSAATAP
                     FRGPLGIMQMPTAPASNLPPNTFMEGISAEAKMNAYQSMMMMNPMSLRQMINIMVAKK
                     KAEAGISFDDVVESMKVRANKRNFKLVGHSPLSKDVAAISGDKNTPRVEIFSFCDAMV
                     AREIVDAVPEFIAFLPCRIAVMEDAKKEIWLVTLDWDVRWLDTSNHPDKLSASLREKA
                     INVRDAIDDIMSAGAKGEL"
BASE COUNT         1500 a         2589 c         2406 g         1416 t
ORIGIN      
        1 tctttggagc tccaaatttc gacaggcaac tcgcctcctc cacctcctgc agcgcccggc
       61 ccagggcctc ccgccgggcg ttctggcact gcaggcgatc tattccggca ccaaccggcc
      121 ggtaagcacc ccggtgatct ggcgcatcta tgtcgtgcac ggcaatgagc cgacgctcac
      181 ggcgacatct gccgaacttg cgcccactct gcccctgccg ccgggtgagt atatggtgca
      241 tctgtcgcaa gggctggctg ccgtcacccg ccgcatcgtc atgaccgcgg caccgctttc
      301 ggagcggctg actttgaacg ccggcggcct tgtcttgcgc gccaagctgg cggaagcgcc
      361 tgtgccggcg agccggcagg ccatctccgt gttcatccct gcgccgaacg attcggaagg
      421 caagctcgtc accaattccc tgaaggcggg ggaactgctc cgtttgcctg aggggaccta
      481 tcacgtggtt tccacctacg ccggatcgaa ctcggtggtg cgcgccgaca ttcaggtgca
      541 gagcggccgc gttacggaag ccacgatgaa ccacaaggcc gcgacgatga ccctgaagct
      601 ggtccgacag gttggcggcg ttgcggtcgc caacacctcc tggacggtac agacccccgg
      661 cggcgacctg atccgcgaag cgctcggagc gttcccgacg atggaattgg ctgaaggcga
      721 gtacgaagtc attgcccgca acgacgggcg ggagttcaag gaacgcatga aggtggtgag
      781 cgggatcaat cgcgaccacg aagtggtcat gcggtgatgt cgcttccctt tccgcgccca
      841 tccacctaat ttcgcgccaa accggagcgc ccgatgcaga ccttcttcat gcagatcaaa
      901 tgcaagctcg gccagaccta caaggtcgcc accgacttgg ccgaagcgga aatcgcctcg
      961 gaaatctact cggtcgccgg caactacgac atcctggcca aattctacgt cgatgacgat
     1021 gtcgacatcg gtcacttcgt cgcccagaag gtacagacgc tgccgggcat ccaggacaca
     1081 ttgacgctga tcactttcaa ggcgttctga cgattcacga cgttttcctc gaattcttgc
     1141 atccgcgccg tcgggaggca taactcgccg cgcggccaag gacgatgtga gtttcgatga
     1201 tgcgcgcgag tgtacccgcc attctgatcc tgcttggcgc atcgatcgtg gccgccgagg
     1261 cccaaccggc gcagggacgc tggccgatgg cggtcggcga gcgatactgc gccggcgagc
     1321 caacacaccc caagggcggc ctcaactggc ggctgtcctg cgctgccgcc tgccctgccg
     1381 gatcagcgct cgtgacgggc tcctgccgcg tgattgcatc cgacaatcag ggcatcgcgc
     1441 tggaaggggc cggaccaagc atcaatggct ggagttgcga ctatgtcgcg ccgcaccacg
     1501 atgtcagccg ttccggcgcg ctgagccggc gcgtgcgcgt gaaggcagag gcccattgcc
     1561 gagagacggc ggcggctccg ccgggccgct ggtcggtacc ggcggaaggc gttcccatcg
     1621 ttattgcccg ttcaggcgaa accatttcgg agaagatccg gttcagcgtc cgcctgtgca
     1681 ataccgaggg tcctgccccg gtcaacgtgc atctgctgtt gcgcgatccg aaccagcccg
     1741 gcattcccga acgaccggcg gcgatgccgg ttccgcccgg ccattgcctg caggtcgacc
     1801 agcccgtggc cgtcttcgtg cagaacggtg caacccagca gaccggactg gccggcacct
     1861 accaactcta caaggatggc acgttcaagc ccagaccgga gattgccagc gcgatcgatc
     1921 ttgctcgcaa cgcgcgggac gaaggagcga tcgccaggcg cgcggtgaag gcgacggcgc
     1981 ctgaactcgt ccgtgcacgc tgcgaaaggc tggacccggc gccgaagggc atctggggac
     2041 gctgcgccat ccccgagatt tcagaactcg gcaattaccg catctgcttc gcgcccaatt
     2101 acgccccgcc ggcgaacacg gaacccaacc accccggcgc tctgctgccc atggtggtga
     2161 atcccaaact ggtccggctt cccctgccgg ccgactactc ggctggcacc ccaggcgcac
     2221 gcgacgtcca caacccgatc caggccaatg cctgccgcga ctacctcaat gtgcgcgagg
     2281 tcaatgtgct gatgctcgaa agcacgatct tcccgccgca gaatgtcgat tcggtgtcgt
     2341 tcacgctgca gaggctgggg taaaggcgtg gcacaagccc cgactggcgc tgccaggccc
     2401 gggaccagtc cttcaagaaa cagcacccaa ccaccatgca gtccggggcg acgcacagta
     2461 cgcccaggac tgtcaccggc tatgcgctac ccccgcgcgc gtttcacgtc cggcggcgtc
     2521 gcttcgtccg caagcattgc cagcgcctcg tcaagcttca tgaccgattg cgcctgtgaa
     2581 ccgagccggc ggatggccac cgtcccttcc tccgcctcgc gcttgccgac agcgaagatc
     2641 acaggcgtct tggccagtga gtgctcgcgc accttgtacc cgatcttctc attcctgaga
     2701 tcggcgcgcg cgcgcagccc ctcccgaagc gcccggccga caaccgcatg ggcgtaatca
     2761 tccgcgtcct gcgtgatggt gcagactgat atctgctccg gcgccagcca gagcggcata
     2821 tgaccaccgt ggctctcgat caggatgccg aggaagcgtt ccatcgagcc gcagatggcc
     2881 cggtggatca tcaccggcac ggtcttctca ccggccgcat cgatgtagaa tgcgccgaac
     2941 ctctccggca ggttgaagtc gacctgcgtg gtgccgcatt gccagtcgcg gccgatggcg
     3001 tcgcgcagcg tgtactcgaa cttcggcccg tagaaggcgc cctcgcccgg attgatcccg
     3061 gtcttgatct tgccgccgga ctgcgcagcg atcttttcca gaacgcgcat cataacgctt
     3121 tcggcgcgat cccacagcgc atccgagccg acacgctttt cagggcgcgt ggacagcttc
     3181 accacgagtt cgtcgaagcc gaagtccgca tagaccgaga gcatcagctc gttgatcttg
     3241 aggcactcgg cttccatctg ctcgtccgtg cagaagatgt gcgcgtcgtc ctgcgtgaag
     3301 ccacgcacgc gcatcagccc gtgcagcgcg ccagacggct catagcgatg cacgacgccg
     3361 aattcagcca ggcgcatcgg caggtcgcgg tagcttttca gcccgtgctt gaaaagctgc
     3421 acatgaccgg ggcaattcat cggcttcagc gcgaagatgc gtcgatcagc atccgcatcc
     3481 tgcgggcgga tgatggcgct ggccgcgacc gagccgaaca tgttgtcgga ataccagccc
     3541 cagtggccgg aggtttccca cagcgacttg tcgagaagct ggggagcgtt gacctcatcg
     3601 tagtcggcgg cgagccggcg gcgcatgtag gcaaccagct cctggaacat tttccagccc
     3661 ttgggatgcc agaatacgac acccggaccc tcttcctgga agtggaacaa gtccatctcg
     3721 cgcccgagcc ggcgatggtc gcgtttctct gcttcctcca gttgcttgag ataggcatca
     3781 agctcgtcct gcttcgcgaa ggccgttccg tagatgcggg tcagcatcgg cttcgaggaa
     3841 tcgccgcgcc agtaggcacc ggcgaccttc atcagcttga aggccgtgcc gatcttgccg
     3901 accgaagtca tgtgcgggcc gcggcagaga tcgagccact caccctgctt gtacatcttc
     3961 agcgtctggt cgtccggaat ggcgtcgacc agttccacct tgaagtgctc gcccttctcc
     4021 ctgaagaagg ccttggcctg gtcgcgcgac cactcctctt tggtgaaggg cgcatccttc
     4081 gcgacgatct cgcgcatctt cttctcgatg gcggcgaaat cctccggcgt gaacggctca
     4141 ttgcgatgga aatcgtaata gaagccgttc tcgatcaccg ggccgatggt gacctgcgtt
     4201 cccggaaaga gcgcctgcac cgcctcggcg agcacatggg cgcaatcgtg gcggatcagt
     4261 tccagcgcgc gcggatcctc gcggttgagg aactcaatct tcgcatcgcg tgcaatcggg
     4321 tcggccagat cgcagacgac cccgtcaatc gccatggcaa cggtgcgttt ggccagagac
     4381 ggcgagatgc ccttggcgat gtcgagaccg gagatgttta cggggaactg gcgcacggcg
     4441 ccatcgggaa acgtcaggga aatcatgtcg ctctcgcttc tgctcactcg ccgcaacaaa
     4501 cggcgtaagc cttgaggaaa atacgggcgc cagtggcgcc cggcgctgcg atacaccaga
     4561 tcgggcaacc gcgccaaggg acagcgccgc acgaaacggc accaatatgg ttagcaatat
     4621 cctaacgcga tcccttcctc tgcggccaaa gccgtgccag attggatcaa atccggctgg
     4681 cgcgctttgt gcctcgccgg cggcatgcgg cgaaattctg tgttccagtg gcgtaacttc
     4741 cggcgtatcg ggcttggcac cgtcctgttg gcggccctga tggcgttcgc ctcagcacag
     4801 cccgcagcgt cgatgacgtt caccctcggc cgttcaggcc cactgccatg ccagcgcgat
     4861 tgcgccgaat tcatcgtggc ggatggcgag atcgggccgg atagcgcggc cgaattcctg
     4921 gcgctgtgga gccggttgcc gcgcaaggac ctgccgctga tgctcaactc gcctggcggc
     4981 aggctggacg gcgcgatggc acttggccgt gcgttgcggc acctgaacgt gacggtcacg
     5041 gtcgcccgtg cccgccgcct cggaacggaa actccgggcg tcgcgcagta tgccgcgcgt
     5101 ctcgacgccg gcatctgtca ttcggcctgc gtctacacac tcgccggcgc ctcccgccgc
     5161 aacatggctc cagggtccga aattggcgtc caccaattct atctcgcgcg tgcgagcgac
     5221 accaagcgcc ggcccaagca gcagtattcg gacgcggact tcggccggtt acagagcacg
     5281 gttgccgcgc tggcgggcta tctggtcgaa atgcaggtgg atttgcgcct gctgacactg
     5341 gccgccgaaa cggagccaag ccgaatccgc gtcctgtcat cgggagaggt cgctgagctg
     5401 cttccggccg ccgtgatggt cgaacgggcg gcggcgccac ccctgtccgc agcgtcgttg
     5461 cacccgtcgc tgtccacgac agcgcccccg tccgtccatc agacaccggg ctggccgctc
     5521 gtcatgcgcg aggggcgtcc gtggctggta ttgcaggcag aaagtcagtc tcgccgcttc
     5581 ggtgtgatct ccaacgaggt cgccatcggc tgccggtcgg agggggccga tttcgtcgca
     5641 accttccgcg aaatcctccc tcctgggcgg ccggcatcgg aaggctccgc gcggatcgcc
     5701 accgggtccg atggaggaac cctggcccac aagccgggcg cggaccacct tcgcggcacg
     5761 atctcgcagg aatcgacgat gactgcggca caatccggca cactggacgt cgacgtcacc
     5821 acagtagcga ccgcgaacta tcccatgcgc atcagctttg ccggcaaggg actgatggac
     5881 ggcatcgacc ggctcgagcg cgcctgcacc gcgcgttgac tggcggagcg cgcgcccgcg
     5941 acaattcgcc acagctaatc ccggtcgctc ttttgtctga gccgcgatgc cggccatggg
     6001 ctggattgcc ggagacgcgg ctcaatcgac atattcttga ttgctaatgc gttcgacgtt
     6061 gcgcttttcc gccagattcg ggcagatttt cgaagctatc tgaccctttg cgcggcgggc
     6121 agcaatcctt cgtccaggta ctgcgccatc ttggcgcagc ccgccgcctc agctaaaaat
     6181 attcgtatca atgaatacga gggagggatc catgaaactt aagaagctgg gccttgctgc
     6241 cgccatcgcc ctggcgatgc acaccgcacc cgttaccgcc caggccgaca aggcactcac
     6301 cgattccgcc gcaagctggg tcaaagtctt cgccgatccc aagttcatcg agctcggcat
     6361 gaagatgttc gatccgaaga tgatgacgga cttcatgacc ctcatggcca accccgccat
     6421 gatggaagcc atgatgaaga tgatgagccc tgagctcatg accgccttca tgaaggtcgc
     6481 catggaccca aagatgatcg aggccatgat gaagatggcc gatccgaagc tgatggaagg
     6541 cttcatgaaa atggccttcg atccgaaaat gatggaagcc atgatgaaga tggctgatcc
     6601 caagatcatg acctcgttca tgaccatgat ggctgacccc aagtggatgg aagccatgat
     6661 gaagatgatg agcccggagg tgatgacggc gttcatgaag atggctctcg atccgaaaat
     6721 gatggaagcc atgatgaaga tggccgatcc caagatcatc gagagcttcg tgcgcgtcat
     6781 gttcgatcca aaaatgatcg agtccatgat gaagatggct gatccgcaga tgatgcagcc
     6841 gttcatggca gcgatgacgg atcccaagat gatggatgcc atgatgaaaa tgatggatcc
     6901 caagctgatg atgcagatga tgacggccat ggccacgatg atgaccagct gggccaacac
     6961 cgctccgggc actgccgcgc cgcagaagag atgagcatgg cgcccgccgg cagggttccg
     7021 gctggccctt gaccttgcct gtcgttcacg caccggagtg ttgccccgat ggtccatcgt
     7081 ttcgcacagg cagtcggcct gtccctgacg ctactggcgg gtgccgccac agcccagacc
     7141 cccgcaccga cgccgcagcc caatacactc gccccggctg gatcagccgc gaccgctccc
     7201 ttccgcggcc cgctcggcat catgcagatg ccgaccgcgc cggcgtctaa cctgccaccg
     7261 aacaccttca tggagggcat ctccgccgag gcgaagatga acgcctacca gtccatgatg
     7321 atgatgaacc ccatgagcct gcgccagatg atcaacatca tggtggcgaa gaagaaggcc
     7381 gaggcgggca tcagtttcga tgacgtcgtg gagtcgatga aggttcgcgc caacaagcgc
     7441 aatttcaagc tcgtcggtca cagtccgctg tcgaaggatg tggcggcgat ctccggcgac
     7501 aagaacacgc cgcgcgtgga gatcttctcg ttctgcgacg ccatggtggc gcgcgagatc
     7561 gtcgatgccg tgcctgagtt catcgccttc ctgccctgcc gcatcgcggt catggaagat
     7621 gcgaaaaaag aaatctggct ggtaacgctc gactgggacg tgcgctggct cgatacctca
     7681 aatcacccgg acaagctgtc cgcctcgctg cgcgaaaagg ccatcaacgt gcgcgacgcg
     7741 atcgacgaca tcatgagcgc gggggccaag ggagagcttt gatgcctgcc ctgcccgcct
     7801 gcccgatcag ggcttagggc gaagcggcgg cttttcgttc gtccccttcc acaacccgta
     7861 gcgccagggc agataccagc gcgcgcgcgc cggcacggcc tcgcagacga a
//
LOCUS       DAOMXN010000114         7906 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_4796_length_7906_cov_11.466692, whole genome shotgun sequence.
ACCESSION   DAOMXN010000114 DAOMXN010000000
VERSION     DAOMXN010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 7906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7906
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4796_length_7906_cov_11.466692"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /locus_tag="PK812_08820"
     CDS             <1..839
                     /locus_tag="PK812_08820"
                     /inference="COORDINATES: protein motif:HMM:NF015551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HRJ69698.1"
                     /translation="PPLGRLVAAIPKPIANAMLAGVLLKLCLAPFVALKVLPLQAAAI
                     LLTWLVFFRLARLYAVPAAMAVTLAILALTGEPVRIESAALWPATQIVSPVFTAEALF
                     SIAIPLFVVTMASQNITGLAVLSTFGFRPNAREGLVATGLGSALIAPFGGPTVNYAAI
                     TAALCAGVDAHAEPARRYVAAVFSGVGYVALVALAGVAATVLTKASPLLVEAAAGLAL
                     MGAFGGAMLGATQDENSRIPAMITFLITASGFSFAGIGGAFWGLAIGWAADFALRARI
                     SA"
     gene            complement(829..1218)
                     /locus_tag="PK812_08825"
     CDS             complement(829..1218)
                     /locus_tag="PK812_08825"
                     /inference="COORDINATES: protein motif:HMM:NF013340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein"
                     /protein_id="HRJ69699.1"
                     /translation="MKPILAATGLILAATGGAQALSLKASWGSAAGCGGQSPAFVLGA
                     VPKGTAKLDFKMVDLDLPSYPHGGGSVAFAGKSSFAAGEVFGAFSSYRGPCPPPGQTH
                     RYEWSVQAIDAAGKVLGTAKTVLPFKR"
     gene            1423..2451
                     /locus_tag="PK812_08830"
     CDS             1423..2451
                     /locus_tag="PK812_08830"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRJ69700.1"
                     /translation="MTTFTRRMLGGAALAAGLAFGAMPASAQTALKFTLDWVFQGPTS
                     PFLVALEKGYYKAEGLDVTMDPGQGSAGALQRVATGAYQIGFADVNSLIEHNAKNPGK
                     EILAVFMAYDFPPFGVYALKKSGIKAPKDLEGKKLGAPVFDASFRLFPAFAKKTGIDK
                     GKVEHVNLTPQLREQSLVQGSVDFISGHYFSSILDLKARGVNVDDVVAMRYSDVGMDV
                     YGNAVIVSPELAAQNPKAVTGFIRATVKAWKEVAANPKIGIEATKKRDPLINEALETE
                     RLDMSLKLNVLTPFVKANGMGDVDPARFARSVKDVAEAFGLPTAPDAAKVFSSAFLPP
                     KAERMVAP"
     gene            2610..3413
                     /locus_tag="PK812_08835"
     CDS             2610..3413
                     /locus_tag="PK812_08835"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009452040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ69701.1"
                     /translation="MTQSNTEPPLVELKGVSLAYGRGDGATLALADATLSIQKGEFIA
                     VVGPSGCGKSTLMKLVTGLLPPTSGEVRVHGERVTRPIKGVGMAFQNPTLMPWRTTMD
                     NILLPLEVVEPHKQRFRANKAEYVAKVEKLLATVGLKGFGEKFPWQLSGGMQQRSNLC
                     RALIHDPEIMMLDEPFGALDAFTREDLWSVMQNLWLERRFTAVLVTHDLREAAYLADT
                     IYVMSKRPGKVILKKTVDIPRPRTLATTFEPHFVDIVHELRDRIHQEYA"
     gene            3413..4189
                     /locus_tag="PK812_08840"
     CDS             3413..4189
                     /locus_tag="PK812_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007768823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ69702.1"
                     /translation="MTDTQRYYLKIAMPWLFMAALFLIWELACVMFSIQEIILPRPSK
                     IFTVMVERFDILLRFCLETLWTTTVGFLLAIGFGLVLGLAIGASPFIYSGLYPLLIAF
                     NAIPKVAIVPILMIWLGVGATPAIITAFVISFFPIVVNVATGLATIEPEMRDVMRSLG
                     ATQYEILTKVGIPRAMPYLFASLKVAITLAFIGSVISETVGGNRGIGFLMLSAGARND
                     APTTFAGLFAIAIMGVLMYAICALIEQRMTKWAFRGEVVG"
     gene            4191..4829
                     /locus_tag="PK812_08845"
     CDS             4191..4829
                     /locus_tag="PK812_08845"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRJ69703.1"
                     /translation="MTQDHVADARFWDRFSRRYAAAKIADMAGYERTLERTGELIRGM
                     DRVLEIGCGTGTTALRLAPAVGSILATDISPKMIAIAQEKAAAQACANVQFQVAAPET
                     VTAPQASFDAVLAFNVLHLVRDRAAALAQIRRLLKPGGLFISKTTCVSELNPLIRAAI
                     PVMRAVGLAPHVAVFPAGTLLKDIAQAGFTGIESERHGSGKKDFRLFVVARA"
     gene            4970..5605
                     /locus_tag="PK812_08850"
     CDS             4970..5605
                     /locus_tag="PK812_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HRJ69704.1"
                     /translation="MRDPIETYMNLVPMVVEQSNRGERAFDIFSRLLRERIIFLTGPV
                     EDGMASLIVAQLLFLEAENPKKEISLYINSPGGVVTSGLSIYDTMQFIRCPVTTLCTG
                     QAASMGSLLLTAGEKDMRFALPNARIMVHQPSGGFRGQATDILIHAEEIRSLKQRLNE
                     IYVHHTGQTYDAIHTALERDNFMTADKAKEFGLIDKVVAKRDADPAEPKPM"
     gene            5864..7135
                     /gene="clpX"
                     /locus_tag="PK812_08855"
     CDS             5864..7135
                     /gene="clpX"
                     /locus_tag="PK812_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019904739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HRJ69705.1"
                     /translation="MSKQTGGDTRSTLYCSFCGKSQHEVRKLIAGPTVFICDECVELC
                     MDIIREESKSSLVKAKDGIPTPKEICKVLDDYVIGQQHAKRVLSVAVHNHYKRLNHAT
                     KHNDVELAKSNILLCGPTGSGKTLLAQTLARILDVPFTMADATTLTEAGYVGEDVENI
                     ILKLLQASDYNVERAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKIMEGTV
                     ASVPPQGGRKHPQQEFLQVDTTNILFICGGAFAGLEKIISNRGKSTSIGFGAKVSAPD
                     DRRMGEVFREVEPEDLLKFGLIPEFVGRLPVVATLEDLDEPALKKILTEPKNALVKQY
                     QRLFEMENVDLTFQDEAIGHIARKAIERKTGARGLRSIMEGILLETMFDLPGLEGVEQ
                     VVISSEVIEGKARPLYIYGDRGEEKQAASSA"
     gene            complement(7245..>7906)
                     /locus_tag="PK812_08860"
     CDS             complement(7245..>7906)
                     /locus_tag="PK812_08860"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HRJ69706.1"
                     /translation="LVERRPANELSLHGAGREWGTGLLIGAGLYTTCVLVLMALGMYR
                     IEGLNPWTYVIPAVAMALSSGLFEELFFRGVLFRSVEDMFGSWVSLAVSSLVFGLLHL
                     ANPAGTIVGAIYISIEAGLLLAAAYMFTRRLWLSIGFHMAWNYTQSAIFSGIVSGNAP
                     QPGLIRSNIKGPDVLTGGSFGMESSLVAFALCTAAGVVLLILAIRRGNIVPGHASGAA
                     P"
BASE COUNT         1406 a         2582 c         2537 g         1381 t
ORIGIN      
        1 ggccgccgct gggccgactc gtcgcggcca ttcccaaacc catcgcgaac gccatgctgg
       61 ccggggtgct gctgaagctc tgcctcgcgc cgttcgtggc gctgaaggtc ctgccgttgc
      121 aggcggcggc catcctgctg acgtggctcg tgtttttccg cctcgcgcgg ctctatgcgg
      181 tgccggcggc catggcggtg acgctggcga tcctggccct gacgggggaa cccgtgcgga
      241 tcgaatccgc cgcgctctgg ccggcgacgc agatcgtctc gccggtattc acggcggaag
      301 cgctgttctc catcgcgatc cccttgttcg tcgtcaccat ggcgtcgcag aacatcacag
      361 gccttgccgt gctctcgacc ttcgggttcc ggccgaacgc gcgggaaggg cttgttgcga
      421 ccggcctcgg atcggcgctg atcgcgccgt tcggagggcc gacggtgaac tacgccgcga
      481 tcacggccgc gctttgcgcc ggggtggatg cccatgccga gcccgcacgc cgctatgtgg
      541 cggccgtgtt ttccggcgtc ggctatgtcg cgctcgtcgc gctggcgggc gtcgcggcaa
      601 cggtactgac gaaagcctcg ccgctgctgg tcgaggcggc ggcagggttg gcgctgatgg
      661 gggcgtttgg cggcgcgatg ctgggcgcga cgcaggatga gaacagccgc attcccgcga
      721 tgatcacgtt tctgatcacc gcgtcgggct tcagtttcgc cggcatcggc ggggccttct
      781 ggggcctcgc gatcggctgg gcggcggatt tcgccctgcg cgcgcggatc agcgcttgaa
      841 cggcagcacg gtcttggccg tgccgagcac cttgccggcg gcgtcaatcg cctgcacgct
      901 ccactcgtag cggtgcgttt ggcccggcgg cgggcagggg ccgcgatagg atgagaacgc
      961 gccgaagacc tcgcctgcgg cgaagctgga cttgccggcg aacgccacgc tgcccccgcc
     1021 gtgcggatag ctgggcaggt cgaggtccac catcttgaag tcgagctttg ccgtgccctt
     1081 gggcacggcg cccagaacga aggcgggcga ctggccgccg caaccagcgg cagaacccca
     1141 ggatgctttc aacgacaagg cctgggcgcc tccggtggcg gcaaggatca ggcccgtggc
     1201 ggcaaggatt ggcttcatga tctgcggtct ccatcagtcc agcacacgat acgtgcatga
     1261 gtgaaagcgg cacaatcgga aaagggccat tgccaacagc ttgtgaattt ctatctccac
     1321 cagctgacgc gccgtggcac gcggcttgct gaaaacggtg acgtggggca gggttgtcca
     1381 aaacacaggc aggcggattt tggggatcaa cgggagaaca cgatgacaac attcactcga
     1441 cgcatgctcg gcggcgcggc actggccgcc ggcctggctt tcggcgccat gccggcttcg
     1501 gcgcagaccg cgctgaaatt cactttggac tgggtgttcc aggggccgac ctcgcccttc
     1561 ctggtggcac tggagaaggg ctactacaag gccgagggcc tcgacgtgac catggatccg
     1621 ggccagggtt cggccggcgc cttgcagcgc gtggccaccg gcgcctacca gatcggtttc
     1681 gcggacgtga actcgctgat cgagcacaat gccaagaatc cgggcaagga aatcctggcc
     1741 gtgttcatgg cctatgactt cccgcccttc ggcgtctacg cactgaagaa gagcggcatc
     1801 aaggcgccga aggaccttga gggcaagaag ctcggcgcgc cggtgttcga tgcctcgttc
     1861 cgcctgttcc cggccttcgc caagaagacc ggcatcgaca agggcaaggt cgagcatgtc
     1921 aacctgaccc cgcagctgcg cgagcagagc ctggtgcagg gttcggtcga tttcatctcc
     1981 ggccactact tctcgtcgat cctcgacctg aaggcgcgcg gcgtgaatgt cgatgacgtc
     2041 gtcgccatgc ggtattccga cgtcggcatg gacgtctatg gcaacgccgt gatcgtttcg
     2101 cccgaactcg cggcgcagaa cccgaaggcc gtgaccggct tcatccgcgc caccgtcaag
     2161 gcgtggaagg aagtcgccgc caacccgaag atcggcatcg aggcgacgaa gaagcgcgat
     2221 ccgctgatca acgaggccct ggagaccgag cgtctcgaca tgtcgctgaa gctcaatgtc
     2281 ctgacgccct tcgtcaaggc gaatggcatg ggcgacgtcg atccggcccg gttcgcccgc
     2341 tccgtgaagg acgtggccga agccttcggc ctgccgaccg cccccgacgc ggccaaggtc
     2401 ttctcctccg ccttcctgcc gccgaaggcg gagaggatgg tcgctccgta aggcctcccc
     2461 ggcccttcac ccacgtctcg tccgccttcg ccccgcacgg ggcaaaggcg ggccgaagcg
     2521 gcccgtgccg ccttccgccc acctgcgggc gaagggccgg cgcgggatga gggcagcccg
     2581 ccgatccacc agacttccgg atgtccgtct tgacccagtc caacaccgag ccgccgcttg
     2641 tcgaactgaa gggcgtcagc ctagcctacg ggcgcggcga tggcgcaacc ctcgcgctcg
     2701 ccgatgccac cctgtccatc cagaagggcg agttcatcgc cgtggtcggc ccgtccggct
     2761 gcggcaagtc cacgctgatg aagcttgtaa ccggccttct gccgccgacg tccggcgagg
     2821 tcagggtgca tggcgagcgg gtgacgcgcc cgatcaaggg tgtcggcatg gcgttccaga
     2881 acccgacgct gatgccctgg cgcaccacga tggacaacat cctgctgccg ctcgaagtcg
     2941 tcgagccgca caagcagcgc tttcgcgcca acaaggcgga atacgtcgcc aaggtcgaga
     3001 agctgctcgc cacggtggga ctcaaggggt ttggcgagaa attcccctgg caattgtccg
     3061 gcggcatgca gcagcgctcg aacctctgcc gcgcgctgat ccacgatccc gaaatcatga
     3121 tgctcgacga accgttcggc gcgctcgacg ccttcacgcg cgaggacctg tggagcgtga
     3181 tgcagaacct gtggctggaa cgccggttca ccgccgtgct cgtgacgcat gacctgcgcg
     3241 aggccgcgta tctggccgac accatctacg tgatgtcgaa gcgcccggga aaagtgatcc
     3301 tgaagaagac cgtcgacatt ccgcgccctc gcacgctggc gacgaccttc gaaccgcatt
     3361 tcgtcgacat cgtgcatgag ctgcgcgacc gcatccatca ggagtacgcg tgatgaccga
     3421 cacccagcgc tactacctga agatcgccat gccctggctg ttcatggcgg cgcttttcct
     3481 catctgggaa ctcgcctgtg tgatgttctc catccaggag atcatcctgc cgcggccgtc
     3541 gaaaatcttc acggtcatgg tcgagcgttt cgacatcctg ctgcgcttct gcctggaaac
     3601 gctgtggacc accacggtcg gcttcttgct ggccatcggc ttcggcctgg tgctggggct
     3661 ggccatcggc gcctcgcctt tcatctattc cggcctgtat ccgctgctga tcgcgttcaa
     3721 tgcgattccg aaggtcgcca tcgtgccgat cctgatgatc tggctcggcg tcggcgcgac
     3781 gccggcgatc atcacggcct ttgtcatttc gttcttcccc atcgtggtga atgtggcgac
     3841 gggccttgcg accatcgagc ctgaaatgcg tgacgtgatg cgctcgctgg gcgcgacgca
     3901 gtatgaaatc ctgaccaagg tcggcattcc gcgcgccatg ccgtatctct tcgcctcgct
     3961 gaaggtggcg atcacgctgg ccttcatcgg ttcggtgatc tcggagacgg tgggcggcaa
     4021 ccgcggcatc ggcttcctga tgctctcggc cggcgcccgc aacgacgcgc cgacgacctt
     4081 cgccggcctt ttcgccatcg ccatcatggg cgtgctgatg tacgcgatct gtgccctgat
     4141 cgagcagcgg atgacgaaat gggccttccg gggcgaagtg gtgggataag gtgacacaag
     4201 accacgtcgc cgatgcccgg ttctgggatc ggttctcccg ccgctacgcc gccgcgaaga
     4261 tcgccgacat ggcgggctac gagcgcacgc tcgagcggac aggcgagctc attcgaggca
     4321 tggaccgcgt tctggagatc ggctgcggca cgggaacgac ggcgctgcgg ctcgcccctg
     4381 cggtcgggag tatcctggcc actgacattt cgccgaagat gatcgccatc gcgcaggaga
     4441 aggcggcggc gcaggcctgc gcgaacgttc agttccaggt tgcggcgccg gagaccgtga
     4501 ccgctccgca ggcctccttc gacgccgtgc tggccttcaa cgtgctgcat ctggtgcgtg
     4561 accgggcggc ggcgctcgcg cagattcgcc gcctgctcaa gcccggcggg ctgttcattt
     4621 cgaagacgac ctgcgtgtcg gaactcaacc cgctcatccg cgccgccata ccggtgatgc
     4681 gcgccgtcgg ccttgcgccc catgtcgcgg tgttcccggc cggaacgctg ctcaaggaca
     4741 tcgcccaggc agggttcacc ggcatcgaga gcgagcgcca cggctccggc aagaaggact
     4801 tccggctgtt tgtcgtcgct cgggcgtgag ccggcggttc cgcacaagtt cacgccgctt
     4861 tgagccgtgc ccctttgcga ttcacccctc cgctactatt tctgtgccag tcctgttccc
     4921 ctgatctgat tctggccgcg ccctgcgcag ccccaacaag agagccgcga tgcgcgatcc
     4981 gatcgaaacc tacatgaacc tcgtgccgat ggtggtcgag cagtcgaacc gcggcgagcg
     5041 cgccttcgac attttctccc gcctcctgcg cgagcggatc atcttcctca ccggcccggt
     5101 ggaggacggc atggcctcgc tgatcgtggc gcagctgctg ttcctggaag cggagaaccc
     5161 gaagaaggaa atctcgctct acatcaattc gcccggcggc gtggtgacct ccggcctgtc
     5221 catctacgac acgatgcagt tcatccgctg cccggtgacg acgctgtgca cggggcaggc
     5281 cgcctctatg ggctcgctgc tgctcaccgc cggcgagaag gacatgcgct tcgccctgcc
     5341 gaacgcgcgc atcatggtgc accagccgtc gggcggcttc cgcggccagg cgaccgatat
     5401 cctgatccac gccgaggaga tccgcagcct caagcagcgg ctgaacgaga tctatgtcca
     5461 ccacacggga cagacctacg acgcgatcca caccgcgctg gagcgcgaca acttcatgac
     5521 agccgacaag gccaaggaat tcggtctgat cgacaaggtt gtggcaaagc gggacgccga
     5581 tccggccgaa ccgaagccga tgtgacggcg gattcgcctc tggcgcgcaa attgcctata
     5641 agcttgcggt gcggcgtgtt gcgtttcagt cccggacagg tcttgccggc ggcgttagtc
     5701 attaatttgt gacaagcgcc gcaatccggc cgcgtttaac ggttcctcca ttgaaccttc
     5761 gggacaatgg cggatgactt cgaaagccgg gtgatttgca ccaccgggcg gatggaaagg
     5821 ccagcggatc gcgcgagcgg tgcgtcgggc gatggagagg gtgatgagca agcagaccgg
     5881 tggtgatacg cgcagcacgc tgtactgctc tttctgcgga aagagccagc atgaggtgcg
     5941 caagctcatc gcaggaccaa ccgtgttcat ctgcgacgaa tgcgtcgagc tgtgcatgga
     6001 catcatccgc gaggaatcca agtcctcgct ggtcaaggcc aaggatggca tccccacgcc
     6061 caaggaaatc tgcaaggtcc tggacgatta cgtcatcggc cagcagcacg ccaagcgcgt
     6121 gctctccgtg gcggtgcaca atcactacaa gcgcctcaac cacgcgacca agcacaacga
     6181 cgtggaactg gcgaagtcca acatcctcct gtgcgggccg accggctcgg gcaagacgct
     6241 gctggcgcag acgctcgccc gcatcctcga cgtgcccttc accatggccg acgccacgac
     6301 cctcaccgag gccggctatg tcggcgagga tgtggagaac atcatcctga agctgcttca
     6361 ggcttccgac tacaatgtcg agcgggcgca gcgcggcatc gtctacatcg acgagatcga
     6421 caagatctcg cgcaagtccg ataatccttc cataacgcgc gacgtttcgg gcgagggcgt
     6481 gcagcaggcg ctcctgaaga tcatggaagg cacggttgcc tccgttcccc cgcagggcgg
     6541 gcgcaagcat ccccagcagg agttcctgca ggtggacacc accaacatcc tgttcatctg
     6601 cggcggcgcc ttcgcggggc tggagaagat catctccaac cgcggcaagt ccacctccat
     6661 cggcttcggc gccaaggtct ccgctccgga cgaccggcgc atgggcgagg tgttccgcga
     6721 ggtggagccc gaggacctct tgaaattcgg cctgatcccc gagttcgtcg gccgcctgcc
     6781 ggtggtcgcg acactggagg acctcgacga gccggcgctg aaaaagatcc tcaccgagcc
     6841 gaagaacgcg ctggtcaagc agtatcagcg cctgttcgag atggagaacg tggacctcac
     6901 cttccaggac gaggccatcg gccacatcgc ccgcaaggcc atcgagcgca agaccggtgc
     6961 gcgcggcctg cgctcgatca tggaaggcat cctgcttgaa acgatgttcg acctgccggg
     7021 cctcgaaggc gtcgagcagg tggtcatctc ctcggaagtg atcgagggca aggcccgccc
     7081 gctctatatc tacggcgatc ggggcgagga aaagcaggcg gcgtcaagcg cctgacccac
     7141 aggcacgtgc ttcgcgaagc ggtccgccgg gccaatccgg cgggccaacg cgccggttct
     7201 gcatggtctc agacggcagg gcctgcccaa ggcagatgcc aaggtcaggg tgctgcaccg
     7261 ctggcgtgac ccggcacgat attgccccgg cgtatcgcca ggatcaacag cacgacgccg
     7321 gctgccgtgc aaagcgcgaa tgcgactaaa gacgattcca ttccgaagct tccgccggtg
     7381 agcacgtctg gccccttgat gttggagcgg atcaggcccg gttgcggggc atttccggat
     7441 acgatcccgg aaaaaattgc cgattgcgtg tagttccagg ccatgtggaa gccgatgctg
     7501 agccaaaggc gtcgcgtgaa catgtaggcg gcggcaagca gcaagccggc ttcgatgctg
     7561 atgtagatcg ctccgacgat tgtgcctgcg ggattggcca agtgcagcag gccaaaaaca
     7621 agtgacgata cggccagcga aacccagctt ccaaacatgt cttcgacgga acggaacaac
     7681 acgccgcgaa agaagagctc ttcgaacagt ccggaactca gcgccattgc aaccgcaggg
     7741 attacatagg tccaagggtt caggccttcg attcgataca tgccaagggc catcagcacg
     7801 agaacgcagg tggtatacag gcccgcgccg atcagcaggc cagttcccca ttcccgaccg
     7861 gcgccatgga gcgagagttc gttggctgga cgccgctcga ccagcc
//
LOCUS       DAOMXN010000115         7862 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_4819_length_7862_cov_7.655181, whole genome shotgun sequence.
ACCESSION   DAOMXN010000115 DAOMXN010000000
VERSION     DAOMXN010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 7862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7862
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4819_length_7862_cov_7.655181"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..341)
                     /locus_tag="PK812_08865"
     CDS             complement(18..341)
                     /locus_tag="PK812_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010336143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF736 domain-containing protein"
                     /protein_id="HRJ69707.1"
                     /translation="MATIGTFKKTGSNEFSGEIVTLSVQAKGVRIVPDLRATGENAPS
                     HRVLVGRAEIGAAWSKRSNEGRDYLGLKLDDPSFNAPIYANLFDDEDGDGYSLIWSRP
                     NRRAD"
     gene            complement(922..1362)
                     /locus_tag="PK812_08870"
     CDS             complement(922..1362)
                     /locus_tag="PK812_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013368632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69708.1"
                     /translation="MIFLGILLSVAAIGFFCWLLLTLAVFALPLFAGLSAATWAYHTG
                     AGWFGAILVGIAAGGLTLGIGQVLLATVRPLWAKFAISAIFVVPAVIAGYHAVHGIVK
                     HTMPSETWQIVFSLVGAVAVGITAFLRVAGMAAPVPASQELARA"
     gene            complement(1505..1672)
                     /locus_tag="PK812_08875"
     CDS             complement(1505..1672)
                     /locus_tag="PK812_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69709.1"
                     /translation="MSFHDAHAFAFSLATTLMAVIVIFRAGDGTLSVTPAAEYDGDSA
                     AIVDEIDPFVP"
     gene            2025..2384
                     /locus_tag="PK812_08880"
     CDS             2025..2384
                     /locus_tag="PK812_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69710.1"
                     /translation="MNLPTLSEILVSEDAASFERLDEGKWIKGRFDSNIRIDQPTHGA
                     GQTHAHVYGRKGAEIGVVNFDGSSSHGTKCRLNDADADALRDRGFAIKAGNIVEWVVL
                     PTQPQMLLGCRRRSRAA"
     gene            complement(2370..3296)
                     /locus_tag="PK812_08885"
     CDS             complement(2370..3296)
                     /locus_tag="PK812_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010336136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2493 domain-containing protein"
                     /protein_id="HRJ69711.1"
                     /translation="MSAYDDDEPHHESSPTDHIAQELQLYGYRPSEDEPDPRPAPEDR
                     IIEGAIADIFDALVATMADTSLDHDLDDLLWSTVNMFHRAVDRVERKLDDNEQGQKRL
                     QREQDGSEVKSVQLESLIEVGQSLIERRDSLEVFRDSAADRYLRVTGSPWSQRTGSRV
                     NHRQLTSAVIDSRDFLTAKRRAETEVLVPAGPKIAFTGGDTADHKLIWAKLDQVHAKH
                     SDMVLLHGGSPKGAEKIAARWAEIRKVPQVAFKPDWTKHAKAAPFKRNDAMLAIMPIG
                     VLVFPGTGIQDNLADKARKLGIPVYRFGSGGA"
     gene            complement(3604..4659)
                     /locus_tag="PK812_08890"
     CDS             complement(3604..4659)
                     /locus_tag="PK812_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRJ69712.1"
                     /translation="MTRLDASDLATRLGQEAEAVCRHYLDAGRRQGNYWQVGDARNTP
                     GRSMFVRLKETVKGPPGKWTDAATGEHGDLLDVIRESCGLIDFADVAREARSFLSLPR
                     PEPAPVTKKRLCTPAPSGSSEAARRLFAMSQPIGGTLVETYLRNRGITALRGTGSLRF
                     HPRCYYRPDDHGRTETWPAMIAAVTDLRGTITGAHRTWLAPDGPDPIRAGKAPIDTPR
                     KAMGNLLGHAVRFGNAGEVMAAGEGIETILSARQVLPHMAMAAALSAAHLAALLFPDD
                     LRRLYIVRDNDPAGISAQDSLAERANAAGIEAIVLSPRLGDFNEDLRHFGTDALRAML
                     RVQLAPQDVARFMSLVA"
     gene            complement(4659..>7862)
                     /locus_tag="PK812_08895"
     CDS             complement(4659..>7862)
                     /locus_tag="PK812_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012454938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="strawberry notch family protein"
                     /protein_id="HRJ69713.1"
                     /translation="AEPEGLPLAYDTRDGLTGEGGRLSDGIYEEYGLQAIRISGSQTH
                     PTKLVQSAAMASVAPPKPSYRPHLPANIHELLSDAQLETVIYAGEAHADHLAGSWTVD
                     ATFDIVTAAREDAANAQRFRRGFMIGDGTGVGKGRQSAAIILDNWMKGRRKAVWISKS
                     DKLIEDAQRDWSALGMERLLVTPLSRFPQGRPVTLPEGILFTTYATLRSDDRGEKVSR
                     VRQIVEWLGSDFDGTIIFDEAHAMANAGGGKGERGDVAASQQGRAGLRLQHALPNARV
                     VYVSATGATTVHNLAYAQRLGLWGGEDFPFSTRAEFVEAIEAGGVAAMEVLARDLRAL
                     GLYTARSLSFDGVEYELVEHALTPEQTRIYDAYAGAFAIIHNHLDAAMEAANITGATG
                     TLNGQAKSAARSAFESAKQRFFGHLLTSMKTPTLIRSIMNDLESGHAAVIQIVSTGEA
                     LMERRLAELPTEEWNDVRVDITPREYVLDYLAHSFPVQLYEPFTDSEGNLSSRPVYRD
                     GHPVESREAVARRDELIAKLASLPPVPGALDQIVQHFGTDLVAEVTGRSRRIVRKGQR
                     LTVENRAAAANLAETQGFMDDAKRILIFSDAGGTGRSYHAELSARNTRLRVHYLLEPG
                     WKADAAIQGLGRTHRTNQAQPPLFRPIATDVKAEKRFLSTIARRLDTLGAITRGQRQT
                     GGQGLFRPEDNLESPYARDALRQLYLLLVRGKVEGCSLGRFESATGLKLMDANGIKDE
                     LPPITTFLNRLLALTIELQGVLFTAFEQLLTAKIEGAIAAGIYDVGLETLTAEGFTVT
                     GRQTIYTHPGTGAETRLLTIAQRERNRPVTLDDALARLFEPGAKLLVNQRSGRAAVQV
                     PETSIMLDDGEIERRVRLIRPMESHAVPVKMMGETHWIEADRNTFAAAWTAEVADVPE
                     FSDSTIHIVAGLLLPIWKRLPNESTRVYRLQTDDGERIVGRKVSPAWAANATATDAIT
                     LAPADAFVALMDGRTILDLAEGLQLRRVRVMGADRVELSGFTDTMRERLTAYGLFHEI
                     ISWKLRMFVPTDANGPVVLERVLGRYPVQHIGEREAA"
BASE COUNT         1355 a         2370 c         2754 g         1383 t
ORIGIN      
        1 ggggccttgc tggtaggtca gtccgcccgg cggtttgggc gggaccagat cagcgagtag
       61 ccgtcaccgt cctcgtcgtc gaagaggttg gcgtagatcg gggcgttgaa gctcggatcg
      121 tcgagcttga ggcccagata gtcgcggccc tcgttggagc gtttggacca ggcggcgccg
      181 atctcggcgc ggccgaccag gacgcgatgg ctgggggcgt tttcgccggt ggcccgcagg
      241 tcgggaacga tgcgcacgcc cttggcctgg acgctgaggg taacgatttc gccgctgaac
      301 tcgttcgagc cagtcttctt gaaggtgccg atggtcgcca tggtaagtct ccgtttttgc
      361 gttttcgagc ccgcaccatg cggcctcgat ggcgatcggt gggccggagg cgaccgacgc
      421 cgcatcgctt gcgaccgaag cgaagcggag gacggccaag gaggactttg ttgtcgcgcg
      481 aggaatgggc ggcacgccca ggggaagaaa gtctgacgcc gccgttgcgg cataggcggt
      541 cgaggcgaag ccggccttcg gccagatcag gccattggag aggccgtgag gtgcgctcgt
      601 tcaacgggac agagagggag acgtcggcga catttcgtga ccgacagggg gataggaccg
      661 gccgttcacc ggcatcgttg cgtcttaccg ggaaaggcag gctgcgctcc ctcccgtatc
      721 cgggcgacca cagaacatgc ccgtcactcg tgttcgagtt ctgccgcggt tcgtatgtgc
      781 cgctgatcga agcacccttg gggggcggtg ttagggcgcg ggtacgatcc ttcgctcctg
      841 cgcagcccca agcgatctgt ggcgatggag gtgccgtgca tcccgctgtt ctggcgttgc
      901 cgccaacggt gtcgagtgcg tctaagcccg cgccagctcc tggctcgctg ggacgggtgc
      961 cgccataccc gcaacgcgca ggaacgccgt gatgcccact gcaaccgcgc cgacgaggga
     1021 gaacacgatc tgccaggtct ccgaaggcat agtgtgtttc acgatgccgt ggacggcgtg
     1081 gtaccccgcg atgacggcgg gtacgacgaa gattgccgag atcgcaaact tggcccagag
     1141 cgggcggacg gtcgcgagca gaacttggcc gatgccgagg gtaagaccgc ccgcggcgat
     1201 gccgacgagg atcgcgccaa accagccagc gccggtgtga taggcccagg ttgcggcgct
     1261 caaacccgcg aacaagggca gcgcgaacac ggccagcgtg aggagcaacc agcagaagaa
     1321 tccgatggcc gcgacggata ggagaatgcc gagaaagatc atggtggtgt ctccgagaca
     1381 aaaaggtctg aaggtcgcgc ctccaccacc accacggcgc gataggaagc atagcagaac
     1441 atcgctgctg acgggagcgg aaaccggcag ggcttccgca tcgcgcgcga tgatcttcgc
     1501 cccgtcatgg gacgaaaggg tcgatttcat cgacgatggc ggcgctgtcg ccgtcatatt
     1561 cggcggccgg tgtcaccgac agcgtgccgt cgccggcgcg gaagatgacg atgacggcca
     1621 tgagggtggt ggcgaggctg aaggcgaagg cgtgtgcatc gtggaaggac atcgatccgg
     1681 ctcctgtgcg aagcggagga ccatcccccg ctgacaggcg cccgatgagt tcggccgagg
     1741 gccgcaatca ccgcgtaggt gaaaccggga gcggcggcac gcgcagcgta gtccgaccct
     1801 ttatgggttg atggcgtcag gccgtcggct gaaccaaagg atcagcgcat ttgaagcggg
     1861 ggtggtctga cggtcgacgg gaagcctgtt cgctacatcc gatcagaaat agccccggat
     1921 atgacctcga ctctccagga ttcgtcgatt tctcaggcca agtaattcat agtggcaccg
     1981 gtcaacaata gaactgggat acgatgttga gattcaaaga gttcatgaac cttccgactt
     2041 tgagcgagat cctcgtgtcg gaagatgccg ccagtttcga gcgcctagat gaaggaaaat
     2101 ggataaaggg gcgcttcgac agtaatattc gcattgatca accgacccat ggtgccggac
     2161 agacccatgc gcatgtatat ggacggaaag gcgccgaaat cggtgttgtc aattttgacg
     2221 gttcatcaag ccacggcacg aagtgtcggt tgaatgatgc cgatgcagac gccctgcgcg
     2281 atcgcggttt cgcaatcaaa gcgggcaaca tcgttgaatg ggttgttctg cctacgcaac
     2341 ctcagatgtt gctgggctgc aggcggcgct cacgcgccgc ctgaaccgaa ccgatagacc
     2401 gggatgccga gcttacgggc cttgtcggcg agattgtcct gaatgcccgt gccggggaag
     2461 acgaggacgc cgatcggcat gatcgccagc atggcgtcgt tgcgcttgaa gggcgcggcc
     2521 ttggcgtgtt tcgtccagtc gggcttgaag gccacctgcg gcaccttgcg gatttcggcc
     2581 cagcgggcgg cgatcttctc ggcgcctttg ggcgaaccgc cgtgcaacag caccatgtca
     2641 gaatgcttgg cgtgaacctg atcgagcttg gcccagatca gcttgtggtc ggcggtgtcg
     2701 ccgccggtga aggcgatctt cgggccggcg ggcaccaaca cttcggtctc ggcgcggcgc
     2761 ttggcggtga ggaagtctct ggagtcgatc actgcggagg tgagctggcg gtggttgacg
     2821 cgcgatccgg tgcgctgcga ccagggtgag ccagtgacgc ggaggtaacg gtcggctgcg
     2881 ctgtcgcgga acacttccag gctgtcgcgg cgttcgatca gcgattgccc gacttcgatc
     2941 aggctttcga gctggacgga tttgacctcc gagccgtcct gttcgcgctg gaggcgcttc
     3001 tgtccctgct cgttgtcgtc cagcttgcgc tcgacccgat cgacggcgcg gtggaacata
     3061 ttgacggtcg accagaggag atcgtcgagg tcgtgatcga ggctggtgtc ggccatcgtg
     3121 gcgacgaggg catcgaagat gtcggcgatg gcgccttcga tgatgcggtc ctccggcgcc
     3181 gggcggggat cgggttcgtc ctcggaagga cggtagccgt agagctgaag ctcctgtgcg
     3241 atatggtcgg tgggtgatga ttcgtggtgg ggttcgtcat cgtcatacgc gctcatggga
     3301 ggctccgtcg gtggaccgcg accgtcgcgg ccttcatggc gacgaagccc gtgggcggga
     3361 cgggcctgca cccggcgcga agcgccgagc cggagcgtga gcggaggatg gcgaagcccg
     3421 gctattttgc ctcgcgatgg aaaggcggct tcgccgccgc cggaaaatag ccgggcgcag
     3481 ccattgccga cccggcccgg ctatgggccg atcgccctct cgaaggccga ggcgcggtcc
     3541 tcttccgata caggggcatc ccggcgggca tggcgatgac cccgccacgg tcctccctgc
     3601 cggctatgcc accagagaca tgaagcgggc gacatcctgc ggggcaagct gcacccggag
     3661 catggcgcgg agcgcatccg tgccgaagtg gcggagatcc tcgttgaagt ccccgagccg
     3721 cggcgacagc acgattgcct cgatcccggc cgcgttcgct cgttcggcca gactgtcctg
     3781 cgcgcttatc cccgccgggt cgttgtcgcg gacgatatag agccggcgaa gatcatcggg
     3841 gaacaggagg gcggctagat gggcagcaga cagggcggcc gccatcgcca tgtgcggaag
     3901 cacctgacgt gccgacagga tggtttcgat gccttcgccc gctgccatca cctcgccggc
     3961 gttcccgaag cggacggcgt gaccgagcag gttgcccatc gccttgcgcg gcgtgtcgat
     4021 cggcgccttg ccagctcgga taggatctgg gccatcaggg gcaagccagg tgcgatgtgc
     4081 gccggtgatc gtgccccgaa gatcggtgac ggcggcgatc attgctggcc aagtttccgt
     4141 ccggccatga tcgtccggtc gataatagca gcgcggatgg aagcggaggc ttccggttcc
     4201 gcgcaaagcc gtaatgccgc gattgcggag atacgtttct acgagcgtgc cgccgatcgg
     4261 ctgcgacata gcgaacagcc gccttgccgc ctcggacgag cctgacgggg ctggtgtgca
     4321 cagtcgcttc ttcgtcaccg gcgcgggttc tggacgcggc agactgagaa aggaacgggc
     4381 ctcccgggcg acatcggcga agtcgatcag gccgcagctc tctcggatca catcgagcag
     4441 atcgccatgt tcgccggtgg cggcgtcggt ccatttgccg ggcggtccct tcaccgtctc
     4501 cttgagccgc acgaacatcg agcggcccgg tgtgttgcgg gcgtcgccga cctgccagta
     4561 attgccctgg cggcggccgg cgtcgagata gtggcggcaa accgcctcgg cctcctggcc
     4621 gagacgggtc gccagatcgg aagcgtcgag ccgggtcatc atgccgcctc gcgctcgccg
     4681 atgtgctgca caggataacg gccgagcaca cgttccagca caaccggccc attggcgtcg
     4741 gtcggcacga acatgcgcag cttccacgag atgatctcgt ggaacaggcc gtaggccgtc
     4801 aggcgctcgc gcatggtgtc ggtgaatccc gacagctcca cgcggtcggc gcccatgacg
     4861 cgaacgcgcc gaagctgaag cccctcggca aggtcgagga tcgttcgccc gtccatcagc
     4921 gccacgaagg cgtcggcagg cgcgagtgtg atcgcgtcgg tcgcggtcgc attggccgcc
     4981 caggccggcg agaccttgcg gccgacgatt cgctcgccgt catcggtctg gagccgatag
     5041 acacgggtgg attcgttcgg cagccgtttc cagatcggca gcaacagtcc ggcgacgatg
     5101 tggatcgtgc tatcggagaa ttccggcacg tccgcgacct cggcagtcca ggcggccgcg
     5161 aaggtgttgc gatccgcctc gatccaatgg gtctcgccca tcatcttcac gggaacggcg
     5221 tgcgattcca tcggccggat cagccggacg cggcgttcga tctcgccatc atcgagcata
     5281 atcgaggttt ctggaacctg gacggcggcg cggcccgacc gctggttgac cagcagcttc
     5341 gcgccgggct caaagagccg ggcgagcgca tcgtccagcg tgaccggacg attgcgctcg
     5401 cgctgcgcga tggtcagcag ccgggtctcc gctcccgtgc ccggatgggt atagatggtc
     5461 tggcggccgg tgacggtgaa gccttccgcg gtcagcgtct ccagcccgac atcgtagatg
     5521 ccggcggcga tggcgccctc gatcttggct gttagaagct gctcgaaggc ggtgaacagc
     5581 acgccttgaa gttcgattgt cagcgccagc agccggttga ggaaggtggt gatcggcggc
     5641 agctcatcct tgatgccgtt cgcgtccatc agcttcaggc cggtggcgga ctcgaaacgg
     5701 ccgagcgagc aaccctcgac cttgccgcgc accagcagga ggtagagctg gcgcaaggca
     5761 tcgcgggcat aggggctttc gagattgtcc tcgggccgga acagaccctg gccgccggtc
     5821 tggcgctggc cacgggtgat ggcgcccaaa gtgtcgaggc gccgggcgat ggtggagagg
     5881 aaccgcttct ccgccttcac atccgtggcg atggggcgga acagtggcgg ctgcgcctgg
     5941 ttggtgcgat gcgtgcgccc gaggccctga atggcggcgt cggccttcca gcccggttcg
     6001 agcagatagt gaacacgcag ccgcgtgttc cgcgccgaaa gctcggcgtg ataggagcgc
     6061 ccggtgccgc ctgcgtccga gaagatcagg atgcgcttgg cgtcgtccat gaacccttgc
     6121 gtctcggcga gatttgcggc cgcagcgcgg ttctcgacgg tcagacgctg gcctttgcgg
     6181 acgatgcgcc gggagcggcc cgtcacctcg gcaacgaggt ccgtgccgaa atgctggacg
     6241 atctgatcga gtgcgccggg caccggcggc aggctggcga gtttggcgat cagctcgtca
     6301 cggcgggcga cggcttcgcg gctctcgacg gggtggccgt cccgatagac cggccgcgac
     6361 gatagattgc cttccgagtc ggtgaacggt tcgtagagct gcaccggaaa agaatgggcg
     6421 aggtagtcga ggacatattc cctcggcgtg atgtcgacgc gaacgtcgtt ccattcctcg
     6481 gtcggcaact ccgctagccg gcgttccatc agcgcttcgc cggtcgaaac gatctggatc
     6541 acggcggcat ggccggattc caggtcgttc atgatcgacc ggatcaaggt cggcgtcttc
     6601 atcgaggtca gcagatgacc gaagaaacgc tgcttcgccg actcgaaggc cgagcgggct
     6661 gcggatttgg cctgcccgtt cagggtgccg gtggcgccgg tgatattggc cgcctccata
     6721 gccgcatcca gatggttgtg gatgatggcg aaggctccgg cataagcgtc gtagatgcgg
     6781 gtctgctctg gcgtcagcgc gtgctcgacc agctcatatt ccacgccgtc gaaggagagc
     6841 gatcgggcag tgtacaggcc gagcgcccga agatcacgcg ccagcacctc catcgctgcc
     6901 acgccgccgg cctcgatggc ctcaacgaac tcggccctgg tcgagaacgg aaaatcctcg
     6961 ccgccccaga ggccgagacg ctgggcatag gcgaggttat gaacggtggt cgcgccggtc
     7021 gccgagacat agaccacacg ggcgttcggc aaggcgtgct ggaggcgaag cccggcgcgc
     7081 ccctgctgcg aggcggcgac gtcgccgcgt tctcctttgc cgccaccggc gtttgccatc
     7141 gcatgggcct cgtcgaaaat gatcgttcca tcgaaatcgg agcccaacca ttcgacgatc
     7201 tgccggacgc gcgaaacctt ctcgccacgg tcgtcggagc gtagcgtggc ataggtcgta
     7261 aataggatgc cttccggcag cgtgaccggc cgtccctgcg ggaagcgcga cagcggcgtg
     7321 acgagcaggc gctccatgcc gagcgccgac caatcgcgct gggcatcctc gatcagcttg
     7381 tcggacttgc tgatccacac cgccttgcgg cggcccttca tccagttgtc gagaatgatg
     7441 gcggcggact ggcggccttt gccgacgccg gtgccatcgc cgatcatgaa gccgcggcgg
     7501 aagcgctggg cgttggcggc atcttcgcgt gcggccgtga cgatatcgaa ggtggcgtcc
     7561 acggtccacg atccggcgag atgatcggcg tgtgcttcgc cggcgtagat gacggtctcg
     7621 agctgtgcat ccgacagtag ttcgtggata ttggccggga ggtggggacg atagctgggc
     7681 ttcggcggtg cgaccgaggc catcgcggcg gattgcacca gtttggtggg gtgcgtctga
     7741 gagcctgaga tacggatggc ctgcaatccg tattcttcat agatgccgtc ggataggcgg
     7801 ccaccctcgc cggtcaggcc gtcccgggtg tcgtaggcga gcggcaggcc ctcgggttcg
     7861 gc
//
LOCUS       DAOMXN010000116         7836 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_4838_length_7836_cov_11.381699, whole genome shotgun sequence.
ACCESSION   DAOMXN010000116 DAOMXN010000000
VERSION     DAOMXN010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 7836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7836
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4838_length_7836_cov_11.381699"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="PK812_08900"
     CDS             complement(<1..318)
                     /locus_tag="PK812_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HRJ69714.1"
                     /translation="MPLARTRGGDRSMNYWPGFVDALSTLILVFTFLLAVFVLAQFFL
                     AREMSGKDDVLTRLNRQLAQLTEILALERSSKQGLESELASLAASLDAARGESERLRG
                     VAGE"
     gene            complement(318..1322)
                     /locus_tag="PK812_08905"
     CDS             complement(318..1322)
                     /locus_tag="PK812_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotA"
                     /protein_id="HRJ69715.1"
                     /translation="MSDTTDALRLSAPRAYLVRMVVFLVLAGLISAVLHKQILAAFWY
                     NPALNGLIFFVLLIGVLLAMRQVGRLFPEVRWVNGVRRGEQRILAAEPPVLLAPMASL
                     LGERAGHEVISTLTLRSVLDSIGARLDESRDTTRYLTGLLVFLGLLGTFWGLIETVGA
                     IGKLVSSMPSGGDATVLFDQLKSGLSQPLAGMGISFSSSLFGLAGSLILGFLDLQAGQ
                     AQNRFFYELEDWLSTMTQDILPVPQPVSPPVPALPSPQQDLKPVLDKLLVAIAALGPD
                     KGNSAAMANLAEGIQALVQHMRSEQQMIRNWVEYQADQQQDVKRLLERLARETVERRP
                     "
     gene            complement(1398..2189)
                     /locus_tag="PK812_08910"
     CDS             complement(1398..2189)
                     /locus_tag="PK812_08910"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HRJ69716.1"
                     /translation="MIRTPLMNVMTAAALKAGKSLRKDFAELPSLKVSRKGPGDFVSQ
                     ADKRAEDILYRELDRARPGYCFRMEESGTVEGPDKTHVWHVDPLDGTTNFLHGFPNFA
                     VSIGLEREGQLVAGVVYNPATDDMYIAEKGQGAFHNNHRMRVSACIEPLDALVACGIP
                     HAGKAYGETFRKELTAVMGKVGGVRRFGACALDVAAVANGQFEGFWERGLNSWDMAGA
                     IVLVREAGGFVSDIRGNGDMLADGSLIVGNESMFALLKSLVSGID"
     gene            complement(2194..2817)
                     /locus_tag="PK812_08915"
     CDS             complement(2194..2817)
                     /locus_tag="PK812_08915"
                     /inference="COORDINATES: protein motif:HMM:NF014622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HRJ69717.1"
                     /translation="MAAPELLLITPDIAAPDGYRDLLDTALAAAPFAAVILRAAGGDD
                     GLLRAAKALAPIIRKRDAAVLLDHPADMRVVARSGADGAHVAFGAPALAEAADLLKPN
                     RMVGVGGLRSKHDAMEAGEADVDYVLFGEPRPDGSLPEPERVIERAQWWAEIFNLPCV
                     VYARDLASIPDLAETGAEFLALGPWVFEAGDPAATVREARRLASMKM"
     gene            complement(2867..3934)
                     /gene="fba"
                     /locus_tag="PK812_08920"
     CDS             complement(2867..3934)
                     /gene="fba"
                     /locus_tag="PK812_08920"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799936.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HRJ69718.1"
                     /translation="MARITLRQLLDHAAENDYGVPAFNINNMEQALAIMAAADAVDAP
                     VIIQASRGARQYANDIMLKHMMDAVCEIYPHIPVCVHLDHGNEPGTCMTAMQAGFTSV
                     MMDGSLKADGKTPGDWDYNVGVTKTVTTMAHLGGISVEGELGVLGSLETGEGEKEDGH
                     GAEGKLSHDQLLTNPDEAVKFVKETKVDALAIAMGTSHGAYKFSRKPDGKVLAMNVIE
                     EIHRRMPGLHLVMHGSSSVPQELQDIINAYGGKMKPTWGVPVEEIQRGIKNGVRKINI
                     DTDNRMAMTGQIRKVLQENPSEFDPRKYLKPAQEAMTALCKQRLQEFNTAGQASKIKK
                     VIPLSDMAKRYAKGELDPKFS"
     gene            complement(3989..5182)
                     /locus_tag="PK812_08925"
     CDS             complement(3989..5182)
                     /locus_tag="PK812_08925"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRJ69719.1"
                     /translation="MANAFKTLDALSPKGKRVLVRVDLNVPMEGGRVSDATRLERILP
                     TIRDIADRGGRVVLLAHFGRPKGRDPKESLKPVAAELARLLGRPVAFADDCIGDTAAS
                     AVAALKDGGVLCLENTRFHKEEEKNEPSFVTELAKLGDAYVNDAFSAAHRAHASTEGL
                     AKVLPAYAGRTMQAELEALDKALGSPKKPVAAIVGGAKVSTKLDLLGNLVKKVDILII
                     GGGMANTFLAARGVKVGKSLCEHDLVATAREIEAKAKAAGCEIVLPVDALLAKEFKAH
                     AAHRVDDVAHVRDDEMILDVGPKTVAHVEAKLGSAKTLVWNGPFGAFELAPFDTGTVA
                     VAHRAAALTKAGALVSIAGGGDTVAALNHAGSGDDFTYISTAGGAFLEWMEGKALPGV
                     EALKA"
     gene            complement(5200..5742)
                     /locus_tag="PK812_08930"
     CDS             complement(5200..5742)
                     /locus_tag="PK812_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69720.1"
                     /translation="MRLFAALLAVVLIGVPAAAQSDPAARFAARPPAPFTVAPSSHPG
                     NDRALVINSRSGKPTAANADGTLCKAAFKAAPSNAKLSQQQINGMMSTPEWRQNARAA
                     LEQAFSVNSGAVFSQAGVSGLEFVGTPKIGPGAAKTRVYLSMAETPAGRTTLSCATLA
                     SELETALPVFRAIRASLTMP"
     gene            complement(5751..6761)
                     /gene="gap"
                     /locus_tag="PK812_08935"
     CDS             complement(5751..6761)
                     /gene="gap"
                     /locus_tag="PK812_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003523203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRJ69721.1"
                     /translation="MAVKVFINGFGRIGRNVLRAIVEGKRKDIEVVGINDLGPVETNA
                     HLLRYDSVHGRFPADVRVEGESIVINGQKIRVTAIKDPAQLPHKELGVDIALECTGIF
                     TSKDKAAAHLKAGAKRVLVSAPADGADYTVVYGVNDAGLKKEHLVVSNGSCTTNCLAP
                     VAKVLNDNFTILHGFMTTVHAYTGDQPTLDTMHKDLYRARAAALSMIPTSTGAAKAIG
                     LVIPELKGKLDGSAIRVPTPNVSVVDLKVVVKKKVTAEKVNEAILKAAKRGPLKGILA
                     VTAEPNVSMDFNHMPASSTFALDQTKVMEEKFVRVLSWYDNEWGFSNRMCDSAVAMAK
                     LI"
     gene            complement(6825..>7836)
                     /locus_tag="PK812_08940"
     CDS             complement(6825..>7836)
                     /locus_tag="PK812_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRJ69722.1"
                     /translation="AGDLPKGLSRGLRAFKRKAAAETAEIATRKTSEMTIDVIAGALP
                     EFVSGSADLTHSNFTQGKRLAPVAPGKFDGRFIHYGIREHAMAAAMNGMALHGGIIPA
                     SGTFLVFSDYCRPSLRLAALMEQRVLHVMTHDSIGVGEDGPTHQPVEHFAALRAIPNL
                     NLLRPCDLTETIEAYEVALASRRRPSVFALSRQNLPQLRKTFTARNLSALGAYEISPA
                     AGKAEVSIFASGSEVSMAVAAQKLLSEKGVLARVVSVPCLDLFLDQPEAYRRKVIGRA
                     KVKIAVEAGIRQGWDSIIGGEGIFIGMNGFGASGPAAKVYQHFGITAAGVADAAMARL
                     KR"
BASE COUNT         1337 a         2613 c         2544 g         1342 t
ORIGIN      
        1 ctctcccgcc acgccgcgca agcgctcgct ttcgcccctt gccgcatcca gcgaggcggc
       61 cagcgatgcc aattcgcttt ccagcccctg cttggaggag cgttccagag cgaggatttc
      121 ggtgagctga gccaactgcc ggttcaggcg ggtcagcaca tcgtccttgc cgctcatctc
      181 gcgcgccagg aagaactggg ccagcacgaa cacggcgagc aggaacgtga agaccaggat
      241 cagcgtcgac agggcatcga cgaagcccgg ccagtagttc atggagcggt caccgccgcg
      301 cgtgcgggcg agtggcatca gggtctccgc tcgacggtct ctcgcgccag ccgttccagg
      361 agccgcttga cgtcctgctg ctggtccgcc tgatactcga cccagttgcg gatcatctgc
      421 tgttccgagc gcatgtgctg gaccagcgcc tggatgcctt ccgccaggtt ggccatggcg
      481 gcactgttgc ccttgtcagg accaagggcg gcgatcgcca cgagcagctt gtccagcacg
      541 ggcttgagat cctgctgcgg cgaaggcagc gcgggcaccg gcgggctgac aggctgcggc
      601 accggcagga tgtcctgcgt catggtcgag agccagtcct ccagttcgta gaagaaccgg
      661 ttctgggcct gccccgcctg gagatcgaga aagccgagga tcagcgaccc ggcaaggccg
      721 aacagcgagg acgagaacga aatgcccatg ccagccagcg gctgcgacag gccggacttc
      781 aactggtcga acaggaccgt cgcgtcgccc ccgctcggca tggacgagac cagttttccg
      841 atcgcgccga ccgtctcgat caggccccag aaggtgccga gcaggccgag gaacaccaag
      901 agtccggtga gatagcgcgt ggtatcgcgc gattcatcca ggcgcgcgcc gatggaatcg
      961 agcaccgagc gcaaggtcag ggtggaaatg acctcgtggc ccgcgcgttc gcccaagagc
     1021 gaagccatcg gcgccagcaa gaccggcggt tcggccgcca ggatccgctg ctcgccgcgc
     1081 cggacgccgt tgacccagcg cacctcggga aacagccggc cgacctggcg catcgccagg
     1141 aggacgccga tgagcaggac gaagaagatc agcccgttga gggccggatt gtaccagaac
     1201 gcggcgagga tctgcttgtg caggacagcg gagatcagcc cggcgagcac caggaacaca
     1261 accatgcgca cgagataggc gcgcggggct gacaggcgga gcgcatcggt cgtatcggac
     1321 attcggcctc ccgggcgaac cggcgcatag tcttaccccg cttcgcgcga agggcaaatg
     1381 accgaaggcc gcgccggtca gtcgatgccc gagaccaggg atttcagcag ggcgaacatg
     1441 ctctcgttgc cgacgatcag cgaaccgtcg gccagcatgt cgccgttgcc acggatgtcg
     1501 gagacaaagc cgccggcctc gcgcaccagc acgatcgcgc ccgccatgtc ccaggaattc
     1561 agcccgcgtt cccagaagcc ctcgaactgg ccgttggcca cagcggccac atcgagcgcg
     1621 caggccccga accggcgcac gccgccgacc ttgcccatca ccgccgtcag ttccttgcgg
     1681 aacgtctcgc cataggcctt gccagcatgc ggaatgccgc aggcgaccag cgcgtccaag
     1741 ggctcgatac aggcggacac gcgcatgcgg tggttgttgt ggaacgcgcc ctgccccttt
     1801 tcggcgatgt acatgtcgtc ggtcgccggg ttgtagacga cgccggcaac gagctgccct
     1861 tcccgctcca gcccaatcga caccgcgaaa ttgggaaagc cgtgcaggaa attggtggtg
     1921 ccgtcgagcg gatcgacgtg ccagacatgc gtcttgtcgg gcccctcgac cgtgcccgac
     1981 tcctccatgc ggaagcaata gcccgggcgc gccctgtcga gttcgcggta gagtatgtcc
     2041 tcggcccgct tgtccgcctg cgagacgaaa tcgccgggac cttttcggga aaccttcagc
     2101 gagggcaatt ccgcgaagtc cttgcgcagg ctcttgccgg ccttcagggc agcggcggtc
     2161 atcacgttca tcaacggcgt gcggatcatc aggtcacatc ttcatgctgg cgaggcgacg
     2221 cgcctcgcgg acggttgctg cgggatcacc cgcctcgaag acccagggac cgagcgccag
     2281 gaactcggcg ccggtttccg caagatcggg aatgctggcc aggtcgcggg cataaacgac
     2341 gcagggcaga ttgaaaatct ccgcccacca ctgcgcacgc tcgataacgc gctcgggctc
     2401 cggcaggcta ccgtccgggc gcggttctcc gaacagcacg tagtccacgt ccgcctctcc
     2461 ggcttccatc gcgtcatgct tggatcgcag gccgccgacg ccaaccatcc ggttcggctt
     2521 cagcagatcc gccgcttcgg ccagagccgg cgcgccgaag gcgacatgcg ccccgtccgc
     2581 gcccgaccgc gcaacaacgc gcatatcggc ggggtggtcg agcaacaccg ccgcatcccg
     2641 cttccggatg atcggggcca gcgccttggc ggccctgagc aggccgtcgt cgccgcctgc
     2701 tgcgcgcaga atgaccgccg cgaacggggc agcggccaga gcggtgtcga gaaggtccct
     2761 gtaaccgtcc ggcgcagcga tgtcaggcgt gataaggaga agttcgggcg cagccatggc
     2821 cccgcgcttc tacgcggggc cgcgacggcg ggcaaggcgt tcaagatcag ctgaacttgg
     2881 ggtccagctc gcccttggca tagcgcttcg ccatgtcgga cagcgggatg accttcttga
     2941 tcttcgaggc ctggccggcc gtgttgaact cctggagacg ctgcttgcag agtgcggtca
     3001 tcgcctcctg cgccggcttg agatacttgc gcggatcgaa ctccgacggg ttctcctgca
     3061 gaaccttgcg gatctggcca gtcatggcca tgcggttgtc ggtatcgatg ttgatcttgc
     3121 gcacgccgtt cttgatgccg cgctggatct cttccaccgg cacgccccac gtcggcttca
     3181 tcttgccgcc ataggcgttg atgatgtcct gcaactcctg cggcaccgag gacgacccat
     3241 gcatcaccag atgcaggccc ggcatgcggc ggtggatttc ctcgatcacg ttcatcgcca
     3301 gcaccttgcc gtccggcttg cgcgagaact tgtaggcgcc gtgcgacgtg cccatggcga
     3361 tagcgagcgc atcgaccttg gtctccttca cgaacttcac cgcctcgtcg ggattggtga
     3421 gaagctggtc gtgcgagagc ttgccttccg cgccgtggcc gtcctccttc tcgccctcgc
     3481 cggtctccag cgaaccgagc acgccgagtt cgccctcgac cgagatgccg ccgagatggg
     3541 ccatggtggt gacggtcttg gtgacgccga cgttgtagtc ccagtcgcct ggcgtcttgc
     3601 catcggcctt cagcgagccg tccatcatca ccgaggtgaa gccggcctgc atcgccgtca
     3661 tgcaggtgcc cggctcattg ccgtggtcga gatgcacgca gaccggaata tgcggataga
     3721 tctcgcagac cgcgtccatc atgtgcttga gcatgatgtc gttggcgtac tggcgcgcgc
     3781 cgcgcgaggc ctggatgatc accggcgcat cgaccgcatc ggccgccgcc atgatggcga
     3841 gcgcctgttc catgttgttg atgttgaagg cgggcacgcc gtagtcgttc tcggcggcat
     3901 gatcgaggag ctgacgcagg gttatgcggg ccatgaaagg tctccttgga ttgaaagaaa
     3961 accctgcgcc ggaggcgcag ggcaataatc acgctttgag ggcttcgaca cccggcaggg
     4021 ccttgccttc catccattcg aggaaggcgc cgccggccgt cgagatatag gtgaaatcgt
     4081 cgccggaacc ggcgtggttc agcgccgcca cggtgtcgcc gccgccggcg atggagacga
     4141 gggcgcccgc tttcgtcaac gccgccgccc gatgcgccac cgcgacggtg ccggtgtcga
     4201 acggagccag ttcaaacgcg ccgaacggac cgttccatac cagcgtcttc gccgacccga
     4261 gtttcgcctc gacatgcgcc accgtcttgg ggccgacatc gaggatcatc tcgtcgtccc
     4321 gcacatgcgc gacatcgtcg acgcggtgcg cggcgtgcgc cttgaactcc ttggcgagca
     4381 gcgcatcgac cggcagcacg atctcgcagc cggcggcttt cgccttggct tcgatctcgc
     4441 gggcggtcgc gaccagatca tgttcgcaca gcgacttgcc gaccttcacg ccgcgcgccg
     4501 ccaggaacgt gttggccatg ccgccgccga tgatcaggat gtcgaccttc ttcaccaggt
     4561 tgccgagcag gtcgagcttg gtggacacct tcgcgccgcc gacgatggct gcgaccggct
     4621 tcttcggcga gccaagcgcc ttgtcgagcg cttccagttc cgcctgcatg gtgcggccgg
     4681 cataggccgg cagcaccttt gccagtccct cggtggaggc atgggcccgg tgcgcggcgg
     4741 aaaaggcgtc gttgacatag gcgtcaccaa gcttcgccag ctcggtgacg aagcttggct
     4801 cattcttttc ctcttccttg tggaagcggg tgttttccag gcagagcacg ccaccatcct
     4861 tcagcgcggc gacggcactg gccgccgtat cgccgatgca atcgtcggcg aaggccaccg
     4921 gcctgccgag caggcgggca agttccgccg ccaccggctt caggctttcc ttcgggtccc
     4981 ggcctttcgg gcggccgaaa tgggctagca ggaccacgcg tccgccccgg tcggcaatat
     5041 cgcggatcgt cggcaggatc cgctcaagac gtgtcgcatc gctcaccctg ccgccttcca
     5101 tcggcacgtt gagatcgacg cgcaccagca cgcgcttgcc tttgggggag agggcatcga
     5161 gggtcttgaa agcattggcc atgaagtgca cctgaagggt tatggcatgg tgagcgaggc
     5221 gcggatcgcg cggaaaacgg gcaacgccgt ctccagctcg gacgcgagcg tcgcgcagga
     5281 cagggtcgtg cggccagccg gcgtttccgc catcgaaagg taaacgcggg tcttcgccgc
     5341 gcccgggccg atcttgggcg ttcccacgaa ctccaggccg ctgacgccgg cttgcgaaaa
     5401 caccgcccct gaattcacgg aaaaggcctg ttccagcgcc gcacgggcgt tctggcgcca
     5461 ctccggtgtg gacatcatgc cgttgatctg ttgctgcgac aacttggcgt tggacggagc
     5521 ggccttgaac gccgccttgc acaaggtgcc gtcggcattc gccgccgtcg gtttgccgct
     5581 cctggaattg atgaccagtg cccggtcatt gccgggatgc gaggacggag ccaccgtgaa
     5641 cggggcaggc ggtcgcgcgg cgaaccgcgc ggccggatca ctctgggcgg ctgccggcac
     5701 gccgatcagc acgaccgcca gcagcgctgc gaagaggcgc acggcgcgcc tcagatcagc
     5761 ttcgccatcg ccacggcgct gtcgcacatg cggttggaga agccccactc gttgtcgtac
     5821 caggacagaa cgcgcacgaa cttctcctcc atcaccttgg tctggtcgag cgcgaaggtc
     5881 gacgacgccg gcatgtggtt gaagtccatc gacacgttcg gctcggcggt tacggcgagg
     5941 atgcccttga gcggaccgcg cttggcggcc ttcaggatcg cctcgttgac cttctcggcg
     6001 gtgaccttct tcttcacgac caccttgagg tcgaccaccg agacgttcgg cgtcggcacg
     6061 cggatggcgg aaccgtcgag cttgcccttc agttccggga tcaccaggcc gatggccttg
     6121 gccgcgccgg tcgaggtcgg gatcatcgac agggccgccg cgcgggcgcg gtagaggtcc
     6181 ttgtgcatgg tgtcgagggt cggctggtcg ccggtgtagg cgtgcaccgt ggtcatgaac
     6241 ccgtgcagga tggtgaagtt gtcgttgagc accttggcca ccggcgcgag gcagttcgtg
     6301 gtgcaggagc cattggagac gacgaggtgc tccttcttca ggccggcgtc gttgacaccg
     6361 tagaccacgg tgtagtccgc gccatcagcc ggggcggaga cgagcacgcg cttggcgccg
     6421 gctttcagat gggccgcggc cttgtccttc gaggtaaaga tgccggtgca ttccagcgcg
     6481 atgtcgacgc cgagttcctt gtgcggcagc tgggcgggat ccttgatcgc cgtgacgcgg
     6541 atcttctggc cgttgatgac gatggactcg ccctcgaccc gcacatcggc cgggaaacgg
     6601 ccgtgcacgc tgtcatagcg cagcaggtgg gcgttggtct cgaccggccc cagatcgttg
     6661 atgccgacga cctcgatgtc cttgcgcttg ccctcaacga tggcgcgcag aacgttgcgc
     6721 ccgatgcggc caaacccgtt gatgaaaacc ttcacagcca tgtcacctgc tccttgtcaa
     6781 aaccgaccct gcgccgctgc agcgcgtagg ggtcccgcaa attctcaccg cttcagccgc
     6841 gccatcgcgg catccgccac gccagcggcc gtaatgccga aatgctgata aacctttgcc
     6901 gccggcccgg acgcgccgaa accattcatt ccgatgaaga ttccctcacc gccaatgatc
     6961 gaatcccatc cttgccggat gccggcctcg acggcgatct tcaccttcgc ccggccgatc
     7021 accttgcgcc ggtaggcttc cggctgatcg aggaagaggt cgaggcaagg cacggacacg
     7081 acccgcgcca ggacaccctt ttccgacagc agtttctgcg cggccaccgc catcgacacc
     7141 tccgaaccgg aggcgaagat cgaaacctcc gccttgccgg ccgccgggct gatctcgtag
     7201 gcaccgagcg ccgagaggtt tctggccgtg aaggtcttgc gcaactgcgg caggttctgg
     7261 cgcgacagcg caaagacgct cggccggcgc cgggaggcca gcgcgacttc ataggcctcg
     7321 atcgtctcgg tcagatcgca ggggcgcagc aggttgaggt tcggaatggc gcggagcgcc
     7381 gcgaaatgct cgaccggctg atgcgtcggc ccgtcctctc cgacgccgat ggaatcgtgc
     7441 gtcatcacat gcagcacccg ctgttccatc agcgcggcga ggcgcagcga gggccggcaa
     7501 tagtcggaaa acaccaggaa cgtgcccgaa gccggaatga tgccgccgtg aagcgccatg
     7561 ccgttcatcg cagcggccat cgcatgctcg cggataccgt agtggatgaa gcggccatca
     7621 aacttgccag gcgcgacagg ggccagccgc tttccttgcg tgaaattcga atgcgtcagg
     7681 tcggcggacc ccgacacgaa ctccggcagc gcacccgcga tgacgtcgat cgtcatttcc
     7741 gacgtcttcc gtgtggcgat ttccgccgtt tccgcggccg ccttccgctt gaacgcccga
     7801 agaccgcgcg acaggccttt cggcaggtcg cccgcc
//
LOCUS       DAOMXN010000117         7809 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_4853_length_7809_cov_9.815837, whole genome shotgun sequence.
ACCESSION   DAOMXN010000117 DAOMXN010000000
VERSION     DAOMXN010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 7809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7809
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4853_length_7809_cov_9.815837"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PK812_08945"
     CDS             complement(<1..226)
                     /locus_tag="PK812_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69723.1"
                     /translation="MAKAFWFVAALAAALWGGANYYPGFWGLIPPEVQAYVPAIAKPY
                     LNFPTPVQAAQQGQPAPGGPAAGPGGQRPGG"
     gene            complement(361..1950)
                     /locus_tag="PK812_08950"
     CDS             complement(361..1950)
                     /locus_tag="PK812_08950"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRJ69724.1"
                     /translation="MLFAFATTHFINAGMGLFGVEAADAYRRVNGAIWSFPPVRLLFY
                     GSLLLHFLIGIKNLALRRNWRMPRLEAVQITLGLLIPIWLADHLVATSGVKAAAGIQQ
                     TYQQMYQIMWPDLARGQSLLLLMVWIHGCIGLAMVLRLKPWFGRWRDVLFGLACAVPL
                     FALAGFLAGMREAKRRFAGPTITEAQVAAFNRLDFYMEWLLGLAFGAALATIAIRFLM
                     ARFRSRLTVRYADGPTLKVAPGATLLEISRLAGIPHASVCGGRARCSTCRVRLLEGSA
                     LQPEPSIAEQRLLARIGAPESVRLACQLRPTEEICVQRLVNAGEATRRENLQQDPFRW
                     GVERRVTVMFADIRGFTSIAEKSVAFDVVFILNRFLSEMSAAIEKAGGRIDKYLGDGL
                     MALFGADGSEDGGSRAALDAVAGMGAALETLNREFEPLLKARLRIGIGLHTGPAILGR
                     IGGGGKAAQLTALGDTVNIAARLEEMTKALAAVCVVSQATLEAAGHSGADMAQHEVTV
                     RGRSEPLSVRALAQFDTLTIA"
     gene            complement(2023..2469)
                     /locus_tag="PK812_08955"
     CDS             complement(2023..2469)
                     /locus_tag="PK812_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015930916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRJ69725.1"
                     /translation="MTLDNEVKSLQALPMFRGVEPAKLKLLAFASERLSYRAGDRFFR
                     QGDVSDSAYVILSGGVDVLLETGADRIRVAQIPRGSIVGEMGVLSGSPRSATIEASEA
                     TEALRIRKDLFFDLLNEFPQIAVAVMRDLAVRLEKTNAQLSALRKG"
     gene            complement(2474..5149)
                     /locus_tag="PK812_08960"
     CDS             complement(2474..5149)
                     /locus_tag="PK812_08960"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRJ69726.1"
                     /translation="MEPSLFKYIWRHSKREQLLILGVVLASLPFYFLSLDLPKRIVND
                     AIQGKAFAGGKEFIRAFEVRFDLPGFLGGGKVTLFDGIDVDRLNFLWGLSAIFFVLVM
                     INGWFKKTINIEKGVIGERMLRRMRFDLFLQLLKFRPEEVRLVKPSEAASIIKDEVEP
                     IGGFIGDAFIQPAFLSTQALTALAFILVQNIWLGMLSVLTVAIQAVIVPALRKKQLML
                     GKERQIASRKLAGKIGELVEGMPAVRTHGTQAYESAEVGGRLGNLFRIRFELYKRKFM
                     VKYLNNLLAQLTPLMFYTIGGYFALKGTLDVGQLVAVIAAYKDIPPPVKELIDWDQQR
                     NDVTIKYEQVVEQFSPSALPPCDPPPATPLTPLDGKKLAIDGLTVTDGRGSALLESAS
                     AHVTLPAHVALLGGPGSGREIFARAAGRQLTRYDGTVKIGESNLLTMDPDTATRLIAF
                     AGSEPALFPTSIRENVAYGLRYRIPELPGDSNPAELRRQSEARRTGNPVARAEDDWFA
                     YDVAGVRDMQALDHVIVSALRTVGLGDDIYRFGLSARIDPARDAALAAELVTARAHIH
                     ERLEAENKTRLIEPFDPDTYNANGSVGENLLFGVTVAQGMTDGALLSKPENRAVLRQH
                     DLRLPLANMGVQIAETMLEIFVGLPPGHALFERYSFINANDLPAYEEIVTRWRNNGKS
                     LEALENTHDMLANLALGYVEPRHRLGLLSEDLKGRIVAARKSLHEAWRAAGASDVEIY
                     HPERFCSAAPVRDNLLFGRVGFGIAGANETIAELLQSALADLKLEEDIYRIGLDYQVG
                     PGGKLLFATQRAGIDLARCLLRRPDILVVEGSLAGLRTADAMAIVERLRQEFGGRTLI
                     VDLPEDATPTGFDFVITFENGRLTGAAPPQSAAGG"
     gene            5403..6683
                     /locus_tag="PK812_08965"
     CDS             5403..6683
                     /locus_tag="PK812_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009494012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69727.1"
                     /translation="MTHHGSFAAHAPLGAYQGPRILIYSHDTFGLGHIRRARAIANAI
                     VGHDSTASIIIVTGSPVVGNFEYADGIDYVRLPGVVKQPDGDYVSLSLRIPLEESVAL
                     REAIIRQTAVAFDPDILIVDKEPTGFRGEILPTLDFVKARGTRIVLGVRDILDEPEAL
                     IPEWERKGAAQALIEYYDNVVVYGLKEVYEPLSCLALPASVAARTTYTGYLRREMPRE
                     PNLTRYPKLTKGDFVLVTTGGGGDGAGLMDWVISAYETHPTPDIPALLVFGPFIPRDI
                     RRAFMERIGRLRFVDAISFDAKLERLMDRAAGVVAMGGYNTFCEILSLDKRALIVPRA
                     KPRLEQTIRARRAAALGLCSVLEDPTDFGRGRRDPAAMAEGISSMLKRGRPSQAEVPN
                     LLSGLESMVNLTSHWLEPKQRRLGVMYPVQAAAQ"
     gene            6693..>7809
                     /locus_tag="PK812_08970"
     CDS             6693..>7809
                     /locus_tag="PK812_08970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010682630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRJ69728.1"
                     /translation="MTQNPGRRILPIMVKGYPRLSETFIAQEILELERLGFEIEIWSL
                     RRPTDQALHPMHRAIRARLKYLPEYLHDAPLRVLRAALAALFRPRLLKLVGVFLRDLA
                     RDFSPNRVRRFGQALVFARELDPALRHVHVHYLHTPASVARYAALLTGMSFSFSAHAK
                     DIWTTQDWEKREKIADSVFGVTCTRGGVDELQRLCLPSDPGRVALVYHGLDLSRFPTP
                     PDRPARRGDVPSDPLRLVAVGRAVAKKGFDDLLQALALLPADLHWRLVHIGSGELRSD
                     LKALSENLGIASRVEFRGALPQEAVIAALREADLFVLPCKEGDAGDRDGLPNVILEAA
                     TQALPILSTNFAGVPEFVTDGVNGRLVPPRAPRALADA"
BASE COUNT         1385 a         2534 c         2495 g         1395 t
ORIGIN      
        1 gtccgcctgg gcgttgacca cctggacctg ccgccggtcc accgggcgcc ggctgtccct
       61 gttgggccgc ctgcacgggc gttggaaaat tcagataggg cttcgcgatg gccggcacgt
      121 atgcctgcac ctccggcggg atcagccccc agaaacccgg ataatagttc gcgccgcccc
      181 aaagtgctgc ggcgagcgct gccacgaacc agaatgcctt tgccatgccc gcttgacgcg
      241 ccttttccaa aatacccgga ttttgattga aacctatagc ttttcagccg caccgaggga
      301 agcggcacaa catgaaattc cggaaaggtt cagcacgcaa tcgcaggaaa tggttgatct
      361 tcacgcaatt gtcagcgtgt cgaactgagc gagcgcccgc acgctcaagg gctcgctgcg
      421 tccgcgcacc gtcacctcgt gctgcgccat gtcggccccg gaatggcccg ccgcttccag
      481 ggttgcctgc gagacgacac agaccgcggc cagcgccttg gtcatttctt ccagccgggc
      541 cgcgatattc acggtgtctc cgagcgccgt cagctgcgcc gccttgccgc cgccgccgat
      601 ccgcccgaga atggctggcc ccgtatgcag cccgatgccg atgcgcaggc gtgccttgag
      661 cagcggctcg aattcccggt tgagcgtttc gagcgccgcc cccatgcccg ccacggcgtc
      721 gagagcggcg cggctgccgc catcctccga gccatccgcg ccgaacagcg ccatcagccc
      781 gtcgcccaga tacttgtcga tgcgcccccc ggccttttca atcgcggcgg acatctccga
      841 aaggaagcgg ttgagaatga acaccacatc gaaggccacc gacttttcgg cgatggaggt
      901 gaagccgcgg atatcggcaa acatcacggt cactcgccgc tccacgcccc agcggaacgg
      961 gtcctgctgg aggttttcgc gccgcgtcgc ctcgccggca ttgacaagcc gctggacgca
     1021 gatttcttcg gtcggcctga gctggcaggc caggcgcacg ctttcgggcg cgccgatgcg
     1081 ggccagcaga cgttgctcgg cgatactcgg ttcgggctgc agggcggagc cttccagcag
     1141 gcgcacgcgg caggtggaac agcgcgcgcg tccgccgcag accgaggcat gcggaatacc
     1201 ggcaagccgg ctgatctcca gcaaggtggc gccgggcgcg accttcagcg ttggtccatc
     1261 ggcgtagcgc accgtcagac gcgaacggaa acgcgccatc aggaaccgga tcgcgatagt
     1321 cgccaaggcc gcgccgaacg ccagccccag cagccactcc atgtagaaat cgagcctgtt
     1381 gaaggccgcc acctgggcct cagtgatggt cggaccggca aaccggcgct tcgcttcccg
     1441 cattccggct agaaaaccgg cgagcgcgaa cagcgggacc gcgcaagcaa gcccgaacaa
     1501 cacatcgcgc cagcgcccga accatggctt gagccgcagc accatcgcca gtccgatgca
     1561 tccgtgaatc cagaccatca gcaacagcaa ggactggccg cgcgccagat ccggccacat
     1621 gatctggtac atctgttggt aggtctgctg gatgccggcg gcggccttga cgccgcttgt
     1681 cgccacaagg tggtccgcaa gccagatcgg gatcagcagg ccgagggtga tctgcacggc
     1741 ttccaggcgc ggcatgcgcc agttgcgccg caacgcgagg ttcttgatgc cgatcaggaa
     1801 atgcagcagc aggctgccat aaaagaggag acggaccggc ggaaaggacc agatcgcacc
     1861 attgacgcgc cgataggcat cggcggcctc gaccccgaac aatcccatgc cggcattgat
     1921 gaagtgcgtt gtggcgaagg caaacagcac cgaccccgtc cagagacgga gactgcgctc
     1981 cgtcggccaa aaccgggaga gactgtccga tttggccacg gttcagccct tgcgcagtgc
     2041 ggaaagctgc gcgttggtct tttcaaggcg gaccgcaagg tcgcgcatca ccgcaacggc
     2101 aatctgcggg aactcgttca gcaggtcgaa gaacaggtcc ttgcggatac gcagcgcctc
     2161 ggtggcttcc gaagcctcga tggtggccga acgcgggctg cctgaaagca cgcccatttc
     2221 cccgacgatc gacccgcgtg gaatttgcgc cacccggatt cggtccgccc cggtctccag
     2281 caggacgtcc acgccgcccg acaggatcac ataggccgaa tccgaaacgt cgccctgacg
     2341 gaaaaatcgg tcgccggcgc gatagctcag ccgttccgag gcgaaggcca gcagcttgag
     2401 ctttgccggc tcaacccccc tgaacatggg cagggcctgc agtgacttga cttcattgtc
     2461 gagggtcatg acgtcatcct cccgccgccg actggggcgg cgcagcgccg gtgagccggc
     2521 cgttttcaaa tgttatgaca aagtcgaatc cggttggcgt ggcatcttcc ggcaggtcga
     2581 cgatcagggt gcggccgcca aattcctggc gcaggcgctc gacgatcgcc atcgcatcgg
     2641 cggttcgcag gccggccagc gagccttcca ccacgaggat gtcggggcgc cgcagcagac
     2701 accgggccag atcgatgcca gcgcgctggg ttgcgaacaa aagcttgccg cccggaccga
     2761 cctggtagtc caggcctatg cgataaatgt cttcctccag cttgaggtca gccagagcgg
     2821 attgcaggag ctcggcaatg gtctcgttgg cgccggcgat gccgaagccc acgcggccaa
     2881 acagcaggtt gtcgcgcacc ggagcggccg agcagaagcg ctcggggtgg taaatctcga
     2941 catcgcttgc gccggccgcg cgccaggcct cgtgcaggct cttgcgcgcc gcgacgatcc
     3001 ggcccttcag gtcttccgac agcaggccca gacgatgccg gggttcgaca tagccgaggg
     3061 ccaggttcgc cagcatgtcg tgcgtgtttt ccagtgcttc gagcgacttt ccgttgttgc
     3121 gccagcgcgt gacgatctcc tcgtaggcgg gcaggtcatt ggcgttgatg aaggaatagc
     3181 gctcgaaaag ggcgtgtcct ggcggcaggc cgacgaagat ttccagcatc gtctcggcga
     3241 tctgcacgcc catgttggcc agcggcaggc gcaggtcatg ctggcgcagg acggcgcggt
     3301 tctccggctt cgacagcagc gcgccgtcgg tcatgccctg cgcgacggtg actccaaaaa
     3361 gcaggttttc gccgaccgag ccgttggcat tgtaggtatc gggatcgaac ggctcgatca
     3421 gccgcgtctt gttctcggct tccagccgct catggatgtg ggcgcgggca gtcaccagtt
     3481 cggccgcgag agcggcatcg cgggccggat cgatgcgcgc cgaaagcccg aacctgtaaa
     3541 tgtcgtcgcc cagtccgacc gtgcgcaagg cgctgacgat cacatgatcg agggcctgca
     3601 tatccctgac cccggccacg tcgtaggcga accagtcgtc ctcagcgcgg gcgacggggt
     3661 tgccagtgcg gcgcgcctcg gactgccgtc gcagttccgc cggattggaa tcgcccggca
     3721 gttccggaat gcgatagcgc aagccgtagg cgacattttc gcggattgac gtcgggaaaa
     3781 gcgcgggctc cgaacccgcg aacgcaataa ggcgggtcgc cgtgtcgggg tccatggtga
     3841 gcagattgct ctcgccgatc ttcactgtcc cgtcgtaccg cgtgagctgc cgtccggcgg
     3901 cgcgggcgaa gatctcgcgt ccgctgcccg gtccgccaag cagcgccaca tgagcgggca
     3961 aggtgacgtg ggcgcttgcc gattccagca gggcggatcc gcgcccatcg gtgaccgtca
     4021 acccgtcgat ggcgagcttc ttgccatcca gcggtgtcag tggcgtggcc ggcggcggat
     4081 cgcagggcgg aagcgcgctg ggcgagaact gctcaaccac ctgctcgtac ttgatggtga
     4141 cgtcgttgcg ctgctgatcc cagtcgatca attccttcac cggcggcggg atgtctttgt
     4201 aggccgcgat gacggcgacg agctggccaa cgtcgagcgt gcccttcagc gcgaaatagc
     4261 caccgatcgt gtagaacatg agcggcgtga gctgcgcgag caggttgttc aggtacttga
     4321 ccatgaactt gcgcttgtag agttcgaacc ggattcggaa gaggttgccg agccgtcctc
     4381 ctacctccgc gctttcgtag gcctgcgtgc cgtgggtccg cactgccggc atgccttcga
     4441 ccaactcgcc gatctttcca gccagtttgc gcgaggcaat ctgccgttcc ttgcccagca
     4501 tcagctgctt cttgcgcagg gctggcacga tcacggcttg aatggcgacg gtcagcaccg
     4561 agagcatgcc cagccagatg ttctgcacca gaatgaaggc cagagccgtg agcgcctgag
     4621 tcgagagaaa ggccggctgg ataaaggcat cgccaatgaa gccgccgatc ggctccacct
     4681 cgtccttgat gatgctggcc gcctccgacg gcttgaccag ccgcacttcc tcggggcgga
     4741 acttgagcag ctgcaggaac agatcgaagc gcatgcggcg cagcatgcgt tcgccgatca
     4801 cgcccttctc gatgttgatc gtcttcttga accagccgtt gatcatgacg agcacgaaga
     4861 agatggcgct caggccccac agaaaattga ggcggtcgac gtcgatcccg tcgaaaagtg
     4921 tgacttttcc gccaccaaga aaacctggca gatcgaagcg gacctcgaag gcgcggatga
     4981 attccttccc gccggcgaag gccttgccct ggatcgcgtc gttgacgatg cgttttggca
     5041 gatcgagcga caggaaatag aacgggagcg acgcaagaac cacgccgaga atcaacagct
     5101 gttcgcgttt gctgtggcgc cagatgtatt tgaaaaggct cggctccatg cctgttccgc
     5161 tcgtttgctg cgctaccccg cgcgaccata gcgcctttgt ggcgcaacgc cagccagctt
     5221 cgtgaacatt gactttgggt gcgccagggt ggatcaaggg cagatcgatt gcgctgagcg
     5281 ggcaggaacg atgctccctc ggattctggg cggcgcgcgg gttgccgcca gaccgtgatt
     5341 tcgagcggtg atcgatggtt cgagcgtttt caggtggcat tcgggtcgcg agcaggcagc
     5401 agatgacaca ccacggcagc ttcgcggcgc atgcgcctct tggggcatac cagggtccgc
     5461 gcatcctgat ctacagccac gacacgttcg gtttggggca tatccggcgt gcgcgcgcta
     5521 tcgccaacgc cattgtcggc cacgattcga cggcgtccat catcatcgtc acgggatcgc
     5581 cggttgtcgg caatttcgaa tatgcggacg gcattgacta tgtgcgcctg cccggcgtcg
     5641 taaaacagcc cgacggcgac tacgtgtcgc tgtccttgcg cattccgctg gaggaaagcg
     5701 tggcgttgcg cgaggcgatc atccgccaga ccgccgtcgc gttcgatccg gacatcctga
     5761 tcgtcgacaa ggaaccgaca gggtttcgcg gcgagatcct cccaaccctg gatttcgtga
     5821 aggcccgcgg cacccgcatc gttctgggcg tgcgcgacat tctggatgag cctgaggccc
     5881 tgatcccgga atgggagcgc aagggagcgg cgcaggcact catcgaatat tatgataatg
     5941 ttgtcgttta tggcctgaaa gaggtctacg agccgctgtc atgccttgcc ttgccggcca
     6001 gtgtggcagc ccggaccacc tataccggct atttgcggcg cgagatgccc cgcgagccga
     6061 acctgacgcg ctatcccaag cttaccaagg gcgatttcgt cctggtcaca accgggggtg
     6121 gtggcgatgg cgctggtctg atggattggg tgatttcggc ctacgagacg catccgaccc
     6181 ctgacattcc cgcgctgctc gtgttcggtc ccttcattcc gcgcgatatt cgccgtgcct
     6241 tcatggagcg catcggacgc ctgagattcg tcgacgccat ctctttcgat gccaagctcg
     6301 aacggctgat ggatcgtgcg gccggcgtgg tggcgatggg cggatacaac acgttttgcg
     6361 aaatcctgtc gctcgacaag cgggcgctga tcgttccgcg cgccaagccg cgcctcgaac
     6421 agacgatccg tgcgcgccgc gccgccgctc tgggcctgtg cagcgtgctt gaagacccga
     6481 ccgatttcgg tcgcggccgg cgcgatccgg cggctatggc tgaaggcatc tccagcatgc
     6541 tcaagcgcgg gcgcccgtcg caggccgagg tgccaaacct gctcagcggc ctcgaatcga
     6601 tggtcaatct gaccagccat tggcttgagc cgaagcagcg ccgcctcggc gtgatgtacc
     6661 cggtccaggc cgccgcgcag taacggcggc gcatgacgca gaatccggga cggcgcatcc
     6721 tgcccatcat ggtcaagggc tatccgcgcc tgtccgagac cttcatcgcg caggaaatcc
     6781 tcgaactcga acgcctcggc tttgagatcg agatctggtc gctgcgccgc ccgaccgacc
     6841 aggccctgca ccccatgcac cgcgccattc gggcgcggtt gaagtatctt ccggaatatc
     6901 tccacgatgc gccgctgcgc gtcctgcgtg ccgctttggc ggcattgttc aggccacgcc
     6961 tgttgaagct ggtcggcgtc ttcctgcgcg accttgcccg cgacttctcg ccaaaccggg
     7021 tgcgcaggtt cggtcaggct cttgtttttg cgcgcgaact ggacccggcc ttacggcatg
     7081 tgcatgtgca ttacctgcac acgccggcct ccgtggcgcg ttacgcagcg ctgctgaccg
     7141 gcatgagttt cagtttctca gcccatgcca aggacatctg gacgacgcag gactgggaaa
     7201 agcgcgagaa gatcgccgac agcgtgttcg gtgtcacctg cacgcgcggc ggcgtggacg
     7261 aactgcaacg cctgtgcctg cccagcgacc ccgggcgcgt tgcgctcgtc tatcacgggc
     7321 tggacctctc gcggtttccc acgccgccgg atcgaccggc gcgccggggc gatgtcccgt
     7381 ccgatccgtt gcgcctcgtc gccgtcggcc gcgccgtcgc gaagaagggg tttgacgatc
     7441 tcctgcaggc gctggcattg cttccggccg atctccactg gcgcctggtg catatcggat
     7501 caggggaatt gcgcagtgat ctcaaggctc tgtccgagaa tcttgggatc gcctcccgcg
     7561 tcgagttccg gggtgccctg ccgcaggagg cagtgatcgc cgcgctgcgc gaggccgatc
     7621 tctttgtcct gccctgcaag gagggcgatg ccggcgaccg agacggcctg ccgaacgtca
     7681 tcctcgaagc ggccacgcag gctctgccca ttctgtcgac gaatttcgcc ggcgttcccg
     7741 agttcgtcac ggatggcgtc aacggtcgcc ttgtgccgcc acgagcgccg cgggcgctcg
     7801 ccgacgcca
//
LOCUS       DAOMXN010000118         7802 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_4866_length_7802_cov_11.395895, whole genome shotgun sequence.
ACCESSION   DAOMXN010000118 DAOMXN010000000
VERSION     DAOMXN010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 7802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7802
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4866_length_7802_cov_11.395895"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="PK812_08975"
     CDS             <1..861
                     /locus_tag="PK812_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69729.1"
                     /translation="VAAGGDATATIKERPEEGTADGDPAKVIGQEDEAAVVESSAGAH
                     EAIEAAEPAPFRLPRPQAPASQVVADGEPEPESDRPKAEGAASTQRPRIDFAPVDEKH
                     RPRKAEPPLAPPRLPKVAAAATDEAPPTAADAVPQPAAKPVDDPFDALEAEFDKFIPL
                     KAAPVAREPEPELGPYVEADVDRPDEALKIALSDEPVLSDGLPPEAPPAPAPGASALR
                     GGLAGPIELEERELAPPGVEVIGSYESGGARYTMYSDGSVVVEIDGKTLKFGSLEQLR
                     EFIEAGAGKG"
     gene            complement(943..1386)
                     /locus_tag="PK812_08980"
     CDS             complement(943..1386)
                     /locus_tag="PK812_08980"
                     /inference="COORDINATES: protein motif:HMM:NF037928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HRJ69730.1"
                     /translation="MSMTLPHEALVLVCDARKALFFKNVGSALAPALEAEATLQAPDN
                     PRSSEQGVERPGKTHQADGRKSGVEPTDFHALAEAAFARDVAEALEKRMAAKAPALFV
                     VAPPRFLASLRTAYGHATKAALRAELDKDLTKLPVKDILRSLSKA"
     gene            1530..2357
                     /locus_tag="PK812_08985"
     CDS             1530..2357
                     /locus_tag="PK812_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69731.1"
                     /translation="MNWSDLIWIVVALSVLQPLLRQRFMDYLRQSRIARFEKMRGSRV
                     VLLVHRQETMRLLGFPLMRYIDVTDSEEVLKAIQLTDPEVPLDLVLHTPGGLALAALQ
                     IARALSDRKGKVTVFVPHYAMSGGTLIALAADEIVLSPHAVLGPIDPQIGEFPAASFL
                     KVVAQKPAAEVDDVTLIKADLAAKAIRQMRDSVRVLLLPHMDAARADVIAEKLTDGHW
                     THDYPVFAEEALALGLPVSTAMPPEILDLMALYPQPVRTSGVEYLPEPRRKEPATRM"
     gene            complement(2362..4458)
                     /locus_tag="PK812_08990"
     CDS             complement(2362..4458)
                     /locus_tag="PK812_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRJ69732.1"
                     /translation="MTGRANFPRAIAPTAKKHPARRVVHGLPLSDDFAWLRADNWRAV
                     LKDPAALPAEIRMHLDSENAHAAALLAPSAALQDVLVREMRGRIREDDAEPPFRDGPF
                     EYYERYRTGGEHPVLCRRPAGGGDETILLDGDAESAGKAFFDLSDHAHAPNHALLAWA
                     ADTQGSEYHTIRVRDVATGEDRDVIEETTGAVVWLKNATGFLYVRLDENHRDNRVYLH
                     RLGTPQSSDRLLHEEQTPGLFVSISETQDRAFALIDIHDHETSEVLLVDPDRPDDVPR
                     SVAPRLVGRRYDVEHHDGRLIIRTNADDAADYKIVTAPAADPHPRHWTELVPHEPGRL
                     IGRMASFASHLVLLTRRDGVPSIVIRDWPSGASHAIAFSEETYALGFTPGFEHDTTSL
                     RFTYSSLSTPQETFDYDMATRGRVLLKKREIPSGHDPARYVVRRINAPALDGETVPVS
                     ILHARTTPLDGTAPCLLAAYGAYGIVIPAGFRSNILSLVDRGFVFAIAHVRGGRDKGQ
                     RWYEDGKRERKTNTFTDYIASAEALVARRFTGTGRIVAQGGSAGGMLMGAIANLRPEL
                     FAGIIAEVPFVDVLNTMLDKDLPLTPPEWPEWGNPIESAADFATIRAYSPYDNVSAQA
                     YPPILIEAGLTDPRVTYWEPAKWAAKLRATMTGGGPILLNTNMDAGHGGASGRLKQLK
                     DDARAYGFALMATGLA"
     gene            complement(4467..4868)
                     /gene="msrB"
                     /locus_tag="PK812_08995"
     CDS             complement(4467..4868)
                     /gene="msrB"
                     /locus_tag="PK812_08995"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HRJ69733.1"
                     /translation="MSSEEKITKSEAEWRAQLTPEQFHVTRRHGTERAFTGPYWNDKS
                     PGTYHCVCCGAPLFASTTKFDSGTGWPSFYDKLGAVSEHEDRSWFMRRTEVRCARCEA
                     HLGHVFPDGPAPTGLRYCINGHALSLTKEEN"
     gene            5265..5672
                     /locus_tag="PK812_09000"
     CDS             5265..5672
                     /locus_tag="PK812_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HRJ69734.1"
                     /translation="MSDNTNFIELAADIVSAYVSNNSVPASDLPALIAQIHGALTHLG
                     GSTQEEPSEPQKPAVSVKKSIAPDYIICLEDGKKFKSLKRHLRTQYNLSPEQYREKWG
                     LPSDYPMVAPNYAAARSRLAKEMGLGQQRRRSR"
     gene            complement(5787..6155)
                     /locus_tag="PK812_09005"
     CDS             complement(5787..6155)
                     /locus_tag="PK812_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69735.1"
                     /translation="MRVKKDARSSPPANPLDGAQEGRLAEIRIRTWAAVDALVARGAA
                     VYQRNRDLPRLLPKLGSADWHAGPLPEPIIEHLKHALVRERALGQCGHRAYNLARHLG
                     LVQALKAEREALACTALARS"
     gene            6554..7100
                     /locus_tag="PK812_09010"
                     /pseudo
     CDS             6554..7100
                     /locus_tag="PK812_09010"
                     /inference="COORDINATES: protein motif:HMM:NF040428.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6456 domain-containing protein"
     gene            complement(7183..7614)
                     /locus_tag="PK812_09015"
     CDS             complement(7183..7614)
                     /locus_tag="PK812_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HRJ69736.1"
                     /translation="MPSLDIEAIRSDIAFLDDWEERYRYIIDLGRSLDPLTAEEHCEA
                     TKVRGCASQVWLVGETLTDDTGARRLMFRGDSDAHIVKGLVALVIALFSGRTAQAILD
                     TDAPGFFAAIGLKEHLTPQRSNGLAAMVERIRAEARAALHA"
BASE COUNT         1285 a         2452 c         2634 g         1431 t
ORIGIN      
        1 gtcgccgccg gcggcgatgc cacggcgact atcaaggagc ggcctgaaga gggcacggca
       61 gacggcgacc ccgcaaaggt cattggtcag gaggacgagg cggcagtggt cgagtcgtct
      121 gctggagcgc atgaggccat cgaggcggcc gagccggcgc ctttccgtct gccccggccg
      181 caggcgcctg catcgcaagt ggtcgctgat ggggagccgg agccggagtc agaccgtccg
      241 aaggcggaag gggcggcttc gacccagcgc ccgcgtatcg atttcgcgcc ggtggatgaa
      301 aagcatcgtc cccgcaaggc cgagccgccg ctggcgccgc cgcgcctgcc gaaggtcgcg
      361 gccgctgcaa ccgatgaagc tccgccgacc gcggcagatg ctgttcccca gccggccgcg
      421 aagccggtgg atgatccctt cgatgcgctc gaggcggaat tcgacaaatt cattccgctc
      481 aaggcggcgc cggtcgccag ggagcccgag cctgaactcg gcccttacgt ggaagccgat
      541 gtcgaccggc cggacgaagc tttgaagatc gcgctatcag atgagccggt gctttcggac
      601 ggcctgccgc ccgaggcgcc gcccgctccc gcacctggcg cttctgcgct gcgcggcggg
      661 ctggccggcc ccatcgaact ggaagagcgc gaacttgcgc cgcccggcgt cgaggtgatc
      721 ggctcgtatg aatcgggcgg tgcgcgctac accatgtatt cggatgggtc ggtcgtggtg
      781 gagatcgatg gcaagacgtt gaaattcggc agcctcgaac agttgcgcga attcatcgag
      841 gccggcgccg gcaaggggtg acacgagccg gcggacacgc gccccgcccc ctgctgtcat
      901 cccgtggacg cgcccgcgtc ggtagcggaa cgtgatcgcg cctcacgcct tggacagcga
      961 gcgcaggata tccttcaccg gcagcttggt gaggtccttg tcgagttctg cccgcagcgc
     1021 agccttcgtc gcgtgaccgt atgcggtgcg cagggatgcc aggaaccgcg gcggcgccac
     1081 gacgaacagc gcaggcgcct tggccgccat gcgcttctcc agtgcctccg cgacatcgcg
     1141 ggcgaaggca gcttcggcaa gcgcatggaa gtccgttggt tcgaccccgg atttgcgtcc
     1201 gtcggcctga tgcgtcttgc cagggcgttc cacgccctgc tcggacgacc gggggttgtc
     1261 cggcgcctgc agcgttgctt cggcttccag ggcgggggcc agtgcgctgc cgacattctt
     1321 gaagaacagg gccttgcgtg cgtcgcagac cagcaccagg gcctcatgcg gaagtgtcat
     1381 cgacatggga tgcgctcctt ttgatcctga caagtctggg cggatggttc ggctgccgcc
     1441 ttgcgttgga tcatttgggc cagccccggc ccttgaccgc gcggctccat gcgtcactgt
     1501 gatggcgcga aatgactgcg gagcgcgtca tgaactggtc cgatctgatc tggatcgtcg
     1561 tcgccctttc cgtgctccag ccgctgctgc gtcagcgttt catggactac ctgcgccaga
     1621 gccgcatcgc ccgcttcgag aagatgcgcg gctcgcgcgt ggtcctgctg gtgcatcggc
     1681 aggagaccat gcgtctgctc ggctttccgc tgatgcgcta catcgacgtc acggattccg
     1741 aggaggtatt gaaggcgatc cagctcaccg atccggaagt gccgctcgat ctggtgctgc
     1801 acacgcccgg cgggctggcg ctggccgcgt tgcagatcgc gcgagccctt tcggatcgca
     1861 agggcaaggt cacggtcttc gtgccgcact acgccatgtc cggcggcacg ctgatcgccc
     1921 tcgcagccga tgaaatcgtg ctctcgccgc atgccgttct ggggccaatc gacccgcaga
     1981 tcggcgagtt cccggcagcc tccttcctca aggttgtggc tcagaagcct gcggccgagg
     2041 tcgacgatgt caccctgatc aaggcggacc tcgccgccaa ggcgatccgg cagatgcgcg
     2101 attccgtgcg cgtcctgctc ttgccgcaca tggatgcggc gcgagccgac gtcatcgcgg
     2161 aaaagcttac ggacgggcac tggacgcacg actatccggt ctttgccgaa gaggccttgg
     2221 cgcttggcct tcccgtaagc acggcgatgc caccggaaat cctggacctg atggcgctct
     2281 atccccagcc cgtgcgcacc agcggcgtgg agtatctgcc ggagccgcgc cggaaggagc
     2341 ccgccacgcg gatgtgactt atcacgccag ccccgtcgcc atcagcgcga atccataggc
     2401 acgggcgtcg tccttcaatt gcttgagccg gccgctggcg ccgccgtgcc ccgcgtccat
     2461 gttggtattg agcaggattg gtccgccgcc ggtcatggtg gcacgcagtt tcgccgccca
     2521 tttcgccggc tcccagtatg tgacgcgcgg atcggtcagc cctgcctcga tcaggatggg
     2581 cggataggcc tgtgccgaca cgttgtcata gggcgaatag gcgcggatgg tggcgaagtc
     2641 tgccgcgctc tcgatcgggt tgccccactc cggccattcc ggtggggtca gcggcaggtc
     2701 cttgtcgagc atggtgttca gcacgtcgac gaagggcacc tcggcaatga tgccggcaaa
     2761 cagctccggc cgcaggttgg cgatcgcgcc catcagcatg ccgcccgccg accccccctg
     2821 cgccacgatc cgccctgtgc cggtgaaccg ccgggcgacc agcgcctcgg cgctcgcgat
     2881 gtaatcggtg aaggtgttgg tcttcctctc tcgcttgccg tcctcgtacc agcgctgtcc
     2941 tttgtcgcgg ccgccgcgca catgggcgat ggcgaagacg aagccgcgat ccaccagaga
     3001 caggatgttg gatcggaatc cggccggaat gacgatgcca taggcgccat aggcggcgag
     3061 caggcagggc gcggtgccgt cgagcggcgt cgtgcgcgca tgcaggatgg aaacaggaac
     3121 ggtttcgcca tccagcgcgg gcgcgttgat gcgacgaacg acatagcgcg ccggatcgtg
     3181 acccgaggga atttcccgct tcttgagcag gacgcggccg cgcgtcgcca tgtcgtagtc
     3241 gaaggtttcc tgcggcgtgg agagagagga ataggtgaag cgcaggcttg tggtgtcatg
     3301 ctcgaagccg ggcgtgaagc cgagagcata ggtctcttcg gagaatgcga tggcatgact
     3361 cgcgccgctc ggccagtctc ggatgacgat ggagggcacg ccgtcgcgcc gcgtcaacag
     3421 cacgagatga ctggcgaagc tcgccatgcg cccgatcagc cggcccggct catgtggcac
     3481 gagttcggtc cagtgcctcg ggtgcggatc ggccgccggc gcggtgacga tcttgtagtc
     3541 cgccgcatca tcggcgttgg tgcggatgat cagccggcca tcgtgatgct cgacatcgta
     3601 gcgccggccc acgaggcgcg gtgcgacgct gcgcggcacg tcgtcagggc ggtccgggtc
     3661 caccagcagc acttcggaag tctcatggtc gtggatatcg atcagagcga aggcccggtc
     3721 ctgggtttcc gaaatcgaaa cgaataaccc cggtgtctgt tcctcgtgca gcagccggtc
     3781 cgagctctgg ggcgtgccca gccggtggag gtatacccgg ttgtcgcggt ggttctcgtc
     3841 gagccggacg tagaggaacc cggtggcgtt cttcagccag accacggccc cggtggtttc
     3901 ctcgatgacg tcgcggtcct cgccggtggc aacgtcgcgc acccggatgg tgtggtactc
     3961 cgagccctgg gtgtccgccg cccaggccaa gagcgcatgg ttcggcgcat gggcgtgatc
     4021 cgaaagatcg aagaaggcct tgcccgcgct ttccgcatcg ccatcgagca ggatcgtctc
     4081 gtcgccgccg ccggccggtc ggcggcacag cacagggtgt tcgccgcctg tacgatagcg
     4141 ttcgtagtat tcgaacgggc catcgcgaaa aggcggttcg gcgtcgtctt cccggatgcg
     4201 tccgcgcatt tccctgacga gtacgtcctg caaagccgct gacggagcga gcagggccgc
     4261 ggcgtgggcg ttctcgctgt cgagatgcat gcggatctcc gcaggcaggg cggcagggtc
     4321 cttcagcacg gcgcgccagt tgtccgcccg cagccaggcg aaatcatccg agagcggaag
     4381 gccgtgcacg acccgtcgcg cggggtgttt ctttgcggtg ggcgcaatcg cgcgaggaaa
     4441 atttgctctc cctgtcatgg cgtgcgttag ttttcttctt tcgtcagcga caaggcatgg
     4501 ccgttgatgc agtagcgcaa ccctgttggc gcaggcccgt ccgggaagac atgcccgagg
     4561 tgcgcctcac accgcgcgca gcggacttcc gtgcggcgca tgaaccagga ccggtcctca
     4621 tgttcgctca cggcgccaag tttatcgtag aaactcggcc atcccgtgcc ggaatcgaac
     4681 ttcgtcgtcg aagcaaacag cggagcgccg caacacacgc agtgataggt gccaggcgac
     4741 ttgtcgttcc agtagggccc cgtgaaggcg cgctcagtgc cgtgccggcg cgtgacatgg
     4801 aactgttcgg gtgtcagctg cgcccgccac tccgcttcgc tcttcgtaat tttttcttcg
     4861 ctggacatgg tcgtttcctg gcgttcctaa gttcctgagg ccaacacagc tatcatgtaa
     4921 cctttgattg gcggctggcc aatcacaaca tggggcgcat tggctcctgc ttaattattg
     4981 cgcaacgttc gattgcctgt ttgcaattga ggagtaacat gagtgaatct tccagatgtc
     5041 ttcgaggttt ttcttgcgaa ctcccgaaat cgtatttata cgagcaaaca atcgtctgct
     5101 gcgaatcgac ttggggaaga tcgctttgca agtgggcgct gacctgatct gactttcccg
     5161 tcggttcccg gtcaaatcca cgcgggataa ggacacggat tttgaggggt gtcccgtctg
     5221 gcttggcaac gtaaacgcat actcgagtca ggaaagcaca tcttatgtca gacaacacca
     5281 acttcatcga gttggccgca gacatcgtct cggcctacgt cagcaacaat tcggttcccg
     5341 cgagcgatct gccggcgctt atcgcgcaga ttcacggcgc tctgacccat ctcggtggta
     5401 gtacccagga agagccgtcc gaaccgcaga agccggccgt ttccgtgaag aagtcgatcg
     5461 cgccggatta catcatctgc ctcgaggacg gtaagaagtt caagtcgctg aagcgtcatc
     5521 tgcgcacgca gtataacctt tctcccgagc agtatcgcga gaagtggggc ctgccgtcgg
     5581 actacccgat ggtcgcgccg aactatgcgg cggcccgttc ccgtctggcg aaggagatgg
     5641 gcctgggcca gcagcggcgc cgcagccgct gatttttccc aggtgatcag aaacgccgcc
     5701 ggaaggcggc gttttttttg ttttgcggga tagggtggcc tggaggatgg aaccaaccga
     5761 tccggagaga tccggagagg ctccgatcaa gaccgcgcca gcgcggtgca tgcgagcgcc
     5821 tcgcgctctg ccttgagtgc ctggaccagc ccaaggtggc gcgccagatt ataggcacgg
     5881 tgcccgcatt gtccgagcgc ccgctccctg accagcgcgt gtttcaagtg ttcgatgatg
     5941 ggttccggca gcgggccggc gtgccagtcc gcacttccaa gcttcggaag cagtctcggc
     6001 aggtcgcgat tgcgctgata gaccgccgca ccgcgcgcga cgagcgcatc caccgccgcc
     6061 caggttcgga tgcggatttc cgccaggcgt ccttcttgcg caccgtccaa cggattggcg
     6121 ggcggtgacg acctcgcatc cttcttgacc ctcatcgcga actcctgtgt tgacaagaac
     6181 taagaatatg atcctatatc atggacagat aggagtaaat acacaggagg ttggtgtgtc
     6241 agagattgat caagccctgc cgtcggggga atccggtgtg tcgggcgcgg aaactctgcg
     6301 cttgttgagt ctcatggcgg ctccgactgc tttcgcgcgt gcggcgtccc gatgggcggc
     6361 agtctatgtc gaacgtgccg gcgtgacgct ggtcgccggg cgcgcgccgg tcgggtcgct
     6421 gcaggacctg aaggcgggtg gatttgcggc aggctctggg cccaagcctg actacaggct
     6481 gaccgaggcg ggtctcgagc tgtcgcggcg gttgctcgtg ggcgacgtgc cggacgatca
     6541 cgcaacgcgc cagatggcgc tggcggtgcg acgaccggcc ggtgactccc cggcgctcct
     6601 ggtcaacgag gcggaaagcc cgctgctctg gctgttccgt cggcgcgggc gcgacggtgc
     6661 cagtctcatt ggcgaggccg agttcgcggc gggcgatcgg ctgcgtgcgg acatagaccg
     6721 ggcggatgat gccccgcctg acggcggact gggagaggcc cgccagtacc ggattttccg
     6781 gcacggccct gagcgcgtcc gaaactgtcg tcgcggcaaa gttgcgggtg gatcgggcgc
     6841 tgtcggcggt cgggccggaa ttctcgggct tgctgctcga tgtctgcggc ttcctgaaac
     6901 cgatcgaccg cattgaacgc gagcgcggct ggccggtccg ctcggcaaag gtggtcatca
     6961 gcctggcatt ggcgcggctc gccaggcact acggttatgc caatcaggcg gaaggccgcg
     7021 cgcgaggcca gttgcgcagc tgggcggaaa gtgggattca gtcaggactt tcgacgggcc
     7081 gccggccgga accagcgtga tcccggatgc attgggccaa ccggcgccgc gccgcactcc
     7141 taacggtcat gcaacgggac cgagcaaggt cacgaagcca ccctaggcat ggagagcggc
     7201 gcgagcctcg gcacggatgc gttcgaccat cgccgcaagt ccgttggagc gttgcggcgt
     7261 gagatgttcc ttcaggccga ttgcggcaaa gaaccccggc gcgtccgtat cgaggatcgc
     7321 ctgcgctgtc cggccggaga acagggcgat caccaaggcg accaggccct tgacgatatg
     7381 ggcatcgctg tcgccgcgaa acatcagacg ccgtgcaccg gtatcgtccg tcagcgtttc
     7441 gcctaccagc cagacctggc tcgcgcagcc tcggaccttg gtcgcctcgc agtgttcctc
     7501 cgccgtcagc gggtcgagac tgcgcccgag atctatgatg tagcggtagc gctcttccca
     7561 gtcgtcgaga aaggcgatgt cggaacggat ggcttcgatg tcgaggctgg gcatggcagg
     7621 gtccggtttt ctcccgctac ctgtcgcccg gcgcgctccc cgtcaaggaa aagcggcggg
     7681 gcgtcgcggc ggaagtggcg gctgcggggg cagggtgctg cgttccggcg gcgcggcgat
     7741 ccttccggtg atctcggagg aaggacgctc gccgtcccgg atgcgccgtg cgacttcctg
     7801 gc
//
LOCUS       DAOMXN010000119         7764 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_4900_length_7764_cov_10.367233, whole genome shotgun sequence.
ACCESSION   DAOMXN010000119 DAOMXN010000000
VERSION     DAOMXN010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 7764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7764
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4900_length_7764_cov_10.367233"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="PK812_09020"
     CDS             complement(<1..329)
                     /locus_tag="PK812_09020"
                     /inference="COORDINATES: protein motif:HMM:NF021376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2076 family protein"
                     /protein_id="HRJ69737.1"
                     /translation="MTPQEREVITGIFERLKPAATQQRDPDAERHIADLIRSQPYAPY
                     VMAQSLFVQEQALANMNQQVQHLQAQVQQLQQAQQQQPASGGFLSGLFGSGQQRPQPQ
                     AQAMAPQG"
     gene            complement(469..2184)
                     /gene="ade"
                     /locus_tag="PK812_09025"
     CDS             complement(469..2184)
                     /gene="ade"
                     /locus_tag="PK812_09025"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HRJ69738.1"
                     /translation="MDRSTLERRIDQGRGVAPADLVLKNTNVFNLVTGEIERTDIAIS
                     GDVIVGTQGTYSGRREIDLSGRFVVPGFIDTHTHVESSLLTPHEFDRCVLPYGVTTVI
                     CDPHEIANVIGAEGIRYFLDCAEQLVLDLRVNLSSCVPATHLETAGARLDAVDLLPFV
                     GHPKVIGLAEFMNFPGVLFKDPQCLEKLQAFAGCHIDGHAPLLRGKDLNGYLSAGIRT
                     DHECTTADEALEKLAKGMAVLIREGSVSKDLHALAGVLTERTSPFCAFCTDDRNPLDV
                     AEEGHLDFLIRTAIALGVSPLAAYRAASWSAARIFGLKDRGLVAPGYRADLVVLETLE
                     ECRVLDVFVAGNRLETAMFETRSPVAAVGRRSMKAQRVSAADFSVPARGNAAPVIGVM
                     PGKIITQHLTLELPVVDGERRIDLAQDVVKVAVVERHGRSRPGEGIGRGFVRGFGLKR
                     GAIASSVGHDSHNICVVGADEADMAVAANRLGEIEGGFVVVESGRVLAELALPVAGLM
                     SLESRDTVRERLIGLRAAATSLGVTLAEPFLQVAFLPLPVIPHLKITDRGLVDVDAFA
                     LLPEA"
     gene            complement(2241..3005)
                     /locus_tag="PK812_09030"
     CDS             complement(2241..3005)
                     /locus_tag="PK812_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRJ69739.1"
                     /translation="MLDLFLPTGVSPLIAILLIALSFVTSAISAVFGLGGGVAMLGAL
                     AGTVQPSVIVAVHAVIQLGSNLGRTIVQRANVIWPAAARFIAGSVAGVALGAYIFTAL
                     PERLFLGLLGAFILLMAWSPKPKVPGLERSGLILGGIVSSFATMFVGATGPFVNAVLL
                     SLGLEKKQLIATHAMCMTVQHALKALAFGLIGFSFRDWLPLIAAMIASGFAGTLLGSK
                     ILDRMSERTFSVILKGLLTVIGLDLLRKALGIPYPF"
     gene            complement(3092..5245)
                     /gene="pnp"
                     /locus_tag="PK812_09035"
     CDS             complement(3092..5245)
                     /gene="pnp"
                     /locus_tag="PK812_09035"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRJ69740.1"
                     /translation="MFDIVRQKLNWAGRVLTLETGRVARQADGAVLASYGETTVLATV
                     VSAKEPKPGIDFLPLTVNYQEKAFAAGRIPGGYFKREGRPSERETLVSRLIDRPIRPL
                     MADGYRCDTQVIVTVLSHDLENDPDILAMVAASAALTLSGAPFMGPIGGARVGCINNE
                     LVLNPTIEQMKESTLDLVVAGTQDAVLMVESEAKELAEDVMLKAVMLGHTGFQPVIDA
                     IIKLAEKAAKEPRDFQAADVSDIEAAVLKIAEQDLRAAYQITAKQDRYNAVDAAKAKV
                     MAEMFPEGGEPKFSKEQVSGVFKELQAKIVRWNILDTHKRIDGRGLADVRQIVAEVGV
                     LPRTHGSALFTRGETQALVVATLGTADDEQFIDELEGTRKETFLLHYNFPPFSVGETG
                     RMGSPGRREIGHGKLAWRAVHPMLPAHHEFPYTIRVVSEITESNGSSSMATVCGSSLA
                     LMDAGVPVKSPIAGIAMGLILEGERYAVLSDILGDEDHLGDMDFKVAGTANGVTSLQM
                     DIKIAGITEEIMKVALDQAKGGRLHILGEMSKALTGARAELGEHAPRIETMKIAVDKI
                     REVIGSGGKVIREIVEKTGAKINIEDDGTIKIASADGKAIKAAINWIKSIASDPEVGQ
                     IYEGTVVKTTDFGAFVNFFGAKDGLVHISQLANRKVAKTTDVVKEGDKVKVKLMGFDE
                     RGKVRLSMKVVDQATGEDIEAKLKAERDAQPRDGE"
     gene            complement(5434..5703)
                     /gene="rpsO"
                     /locus_tag="PK812_09040"
     CDS             complement(5434..5703)
                     /gene="rpsO"
                     /locus_tag="PK812_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006473093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HRJ69741.1"
                     /translation="MSITAERKNALVSEYATKKGDTGSPEVQVAILSERIANLTEHFK
                     SHVKDNHSRRGLLKLVSQRRRLLDYVKSKDEARYKTLIERLGIRR"
     gene            5888..6232
                     /locus_tag="PK812_09045"
     CDS             5888..6232
                     /locus_tag="PK812_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69742.1"
                     /translation="MRLAALALLFAAAPALAEDFGAWKPLTNPFPSTGGGGVMIHDYD
                     PVVSGKTCTTDFSARSGQDRFDGEVVFDAAETQGGVLCANGRWRMKDGSATGTTPFRV
                     FIRDGVKRGDPL"
     gene            complement(6253..7227)
                     /gene="truB"
                     /locus_tag="PK812_09050"
     CDS             complement(6253..7227)
                     /gene="truB"
                     /locus_tag="PK812_09050"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HRJ69743.1"
                     /translation="MTDAPNMQPRPPRAEQPRQVKKIRRDVSGWIVLDKPVGMTSTHA
                     VSIAKRLFNAKKAGHAGTLDPLASGLLPLAFGEATKTVPFVMDGEKAYRFTVKWGAET
                     DSDDADGSVTATSERRPAQEDIENALPFFIGEISQVPPKFSALKIDGERAYDLARDGE
                     EVVLEARTVFIRSLKLVESDADTAVFAAECGKGTYVRAIARDLGRRLGCLGHVVALRR
                     TRVGPFHETDAITVDELRAAGEQGPESDDLRRCLLPVETALADLPEVRVGQHEATRLA
                     NGQPVILRGRDAPATLGTVYVTCHGRLVALAEAEKGQLLPKRAFHLPG"
     gene            complement(7224..7637)
                     /gene="rbfA"
                     /locus_tag="PK812_09055"
     CDS             complement(7224..7637)
                     /gene="rbfA"
                     /locus_tag="PK812_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018093862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HRJ69744.1"
                     /translation="MKRHDPVPGLPSQRQLRVGEQVRHVLADILQRGEIHDPFIEGHV
                     ITVPEVRMSPDLKLATAYVMPLGGKDEAEVVKALNRLARAFRGALAKRLREMRSMPEV
                     RFRLDDRFDAASHIDTLLARPEVARDLAKPAKSEE"
BASE COUNT         1329 a         2525 c         2603 g         1307 t
ORIGIN      
        1 ccctgcggcg ccatcgcctg ggcctggggc tgcgggcgct gctggccgct gccgaacagt
       61 ccggacagga agccgccgga tgccggctgt tgctgctggg cctgctgaag ctgctggacc
      121 tgcgcctgca agtgctgaac ctgctggttc atgttggcca gcgcctgctc ctgcacgaac
      181 agggactgcg ccatcacata gggcgcatag ggctgcgacc ggatcagatc ggcgatatgc
      241 cgctccgcat cggggtcgcg ctgctgggtc gcggccggtt tcagccgctc gaagatgccg
      301 gtgatcacct cgcgctcttg cggggtcatg atcgatgtcc tcctgaagtt ggccgccggc
      361 ggggccgccc ccaccgaatg ccgacggctg agtagatggg caatgtccgc ccgattgcca
      421 gagggtgccc aatgattttc ctgaaagacg tgcgccagcg cacatcgatc aggcctccgg
      481 cagcagcgcg aaggcatcga cgtcgacaag tccacggtcg gtgatcttga gatgcgggat
      541 gaccgggagc ggcaggaagg cgacctgaag aaacggctca gccagcgtga cgcccaggct
      601 tgtcgccgcc gcgcgcaggc cgatcagccg ctcgcgcacg gtgtcgcggc tttccaggct
      661 catcagcccg gcgaccggca gcgccagttc cgccagcacg cggccgctct cgaccacgac
      721 gaagccgcct tcgatctcgc cgagccggtt cgccgccacc gccatgtccg cctcgtccgc
      781 gccgacgacg cagatgttgt gactgtcgtg gccgaccgag gaggcaatgg ctccgcgctt
      841 cagcccgaag cccctgacga agccgcgccc gattccctcg cccggccgcg accggccatg
      901 ccgttccacg acggcgacct tgaccacgtc ctgcgcgaga tcgatgcggc gctcgccgtc
      961 cacgaccggc agttccagcg tgaggtgctg ggtgatgatc ttgcccggca tgacgccgat
     1021 gaccggggcc gcatttccgc gcgccggaac agaaaaatcc gccgccgaca cccgctgcgc
     1081 cttcattgag cgacggccaa cggccgcaac gggcgaacgc gtctcgaaca tcgcggtctc
     1141 gagccggttc ccggccacga acacgtcgag gacccggcat tcttcgagcg tttcgagaac
     1201 cacgagatcg gcgcggtagc ccggcgccac caacccccga tccttcaacc cgaagatgcg
     1261 cgccgccgac cagctcgccg cccggtaggc ggccagcggc gacacgccaa gcgcaatggc
     1321 ggtacggatg aggaagtcga gatggccctc ctccgccaca tccagcggat ttcgatcatc
     1381 ggtgcaaaac gcgcagaagg gcgaggttct ttcggtcaag accccggcca gcgcatgcaa
     1441 atccttggaa accgagcctt ctcggatcaa caccgccatg cccttggcca gcttctcgag
     1501 cgcttcatcc gccgtggtgc attcgtggtc agtccggatg ccggcggaaa ggtatccgtt
     1561 gaggtccttg ccgcgcagca gcggggcatg gccgtcgata tgacaaccgg cgaaagcctg
     1621 aagcttttca agacattgcg ggtccttgaa gagcacgcca ggaaagttca tgaactcggc
     1681 aaggccgatc accttggggt ggccgacgaa gggcagaagg tccaccgcat cgagccgcgc
     1741 gccggcggtc tcaagatgag tcgcggggac gcagctcgac aggttgacgc gcaggtccag
     1801 gaccaattgt tccgcgcaat caaggaagta gcgtataccc tcagcgccga tgacattggc
     1861 gatctcatgc ggatcgcaga tcaccgtggt gaccccatag gggagcacgc accggtcgaa
     1921 ctcatgcggg gtcagcagcg aggattcgac atgggtatgg gtgtcgataa aaccgggcac
     1981 cacgaaacga ccggaaagat cgatctcgcg ccggccggaa tacgtgccct gcgtcccgac
     2041 gatcacatcg cccgagatgg cgatgtcggt ccgctcgatc tctcccgtca cgagattgaa
     2101 aacattagtg tttttcaaga ccagatcggc cggcgcgacg cccctgccct gatcgatgcg
     2161 cctttcgagc gtgctgcggt ccatatgact cctccaagcg cagaagctag cgggcttttc
     2221 ggtaaacgga accggaaacg tcagaacgga tagggaatgc ccagcgcctt gcgcaacagg
     2281 tcgaggccaa tcaccgtcag caaccccttc aggatgacgg aaaaggtccg ttccgacatg
     2341 cggtcgagga ttttcgatcc gagcagcgtc ccggcgaaac ccgaggcgat catcgcggcg
     2401 atgagcggca gccagtcgcg gaaggaaaag ccgatcaggc cgaaggccag cgccttcagc
     2461 gcatgctgca ctgtcatgca catcgcatgc gtggcgataa gctgtttctt ttcaaggccc
     2521 aggctcagca gcacggcgtt cacgaacggc ccggtggcgc cgacgaacat ggtggcgaag
     2581 gacgagacga tgccgccgag gatcagcccg gaccgttcca gccccggcac cttgggcttg
     2641 ggactccagg ccatcagcag gatgaacgcg cccagaaggc cgagaaacag ccgctccggc
     2701 agggcggtga atatgtaagc gccgagcgcc acacccgcca ccgagccggc gatgaagcgc
     2761 gcggcggccg gccagatcac gttggcccgc tgcacgatgg tgcggccgag gttcgagccg
     2821 agctggatca ccgcatgcac cgcgacgatg acgctgggct gcaccgtgcc ggccagcgcg
     2881 ccgagcatcg ccacgccgcc gccgaggcca aatacggccg aaatcgccga ggtgacgaag
     2941 gacagcgcga tcagcaggat cgcgatcagc ggcgaaacgc cggtggggag gaagagatcg
     3001 agcatggcag ctctgtcgcg ccgccagtcc gtgaagtcaa agaaaaaggc cggcttgcgc
     3061 cggccttggg atcgtcaccc gctcagggcg ctcactcccc gtcgcggggt tgcgcgtcgc
     3121 gctcggcctt gagcttggcc tcgatgtcct cgccggtggc ctgatcgacc accttcatgg
     3181 acaggcgcac cttgccgcgc tcgtcgaagc ccatcagctt caccttcacc ttgtcgcctt
     3241 ccttcaccac gtcggtggtc ttggcgacct tgcggttggc gagctgcgag atgtggacga
     3301 ggccgtcctt ggcgccgaag aaattgacga aagcaccgaa atccgtggtt ttcaccacgg
     3361 ttccctcgta aatctggccg acttccggat cggatgcgat ggatttgatc cagttgatcg
     3421 ccgccttgat ggccttgccg tcggccgagg cgatcttgat ggtgccgtcg tcctcgatgt
     3481 tgatcttggc gccggtcttc tcgacgatct cgcggatgac cttgccgccc gagccgatga
     3541 cctcgcggat cttgtccacg gcgatcttca tggtctcgat gcgcggggca tgctcgccga
     3601 gttcggcccg ggcgccggtc agggccttgg acatctcgcc gaggatgtgc aggcggccac
     3661 ccttggcctg atcgagggcg accttcatga tctcctcggt gatgccggcg atcttgatgt
     3721 ccatctgcag cgaggtgaca ccgttggcgg tacccgccac cttgaagtcc atgtcgccga
     3781 ggtgatcctc gtcgccgagg atgtcggaga gcacggcgta acgctcgccc tcaaggatca
     3841 ggcccatggc gatgccggcg atcggcgact tgaccggaac gccggcgtcc atcaacgcga
     3901 gcgaggagcc gcagaccgtc gccatcgagg acgaaccgtt cgactcggtg atctcggaga
     3961 cgacgcggat cgtatagggg aactcgtggt gggccggcag catcgggtgc acggcgcgcc
     4021 aggcgagctt gccatggccg atctcgcggc gaccgggcga acccatgcgg ccggtctcgc
     4081 ccacggagaa gggcgggaag ttgtagtgca gcaggaaggt ctccttccgg gtgccttcca
     4141 gctcgtcgat gaactgctcg tcgtcggccg tgccgagggt ggcgacgacc agcgcctgcg
     4201 tctcgccacg cgtgaacagg gcggagccgt gggtgcgcgg cagcacgccg acttccgcca
     4261 cgatctggcg cacgtccgca aggccgcgac cgtcgatgcg cttgtgggtg tcgaggatgt
     4321 tccagcgcac gatcttggcc tggagttcct tgaagacgcc ggagacctgt tccttgctga
     4381 acttcggttc gccgccctcg gggaacattt ccgccatcac cttggccttg gcggcgtcaa
     4441 cggcgttgta gcggtcctgc ttggcggtga tctggtatgc ggcgcgcagg tcctgctcgg
     4501 cgatcttgag aaccgcagcc tcgatgtccg acacatcggc ggcctggaag tcgcgcggct
     4561 ccttggcagc cttttccgcg agcttgatga tcgcgtcaat gaccggctgg aagccggtgt
     4621 ggccgagcat gacggccttg agcatgacgt cctcggcaag ctccttggct tccgactcga
     4681 ccatcagaac cgcgtcctgc gtgccggcga cgacgagatc gagggtcgat tccttcatct
     4741 gctcgatggt cgggttgagc accagctcgt tgttgatgca gccgacgcgc gcgccgccga
     4801 tcgggcccat gaagggcgcg ccggacagcg tgagcgccgc cgaagcggcg accatggcga
     4861 ggatgtccgg gtcgttctca aggtcatgcg agaggacggt gacgatcacc tgcgtgtcgc
     4921 agcggtagcc gtccgccatc agcgggcgga tcggacggtc gatcaggcgg gagacgagcg
     4981 tctcgcgctc gctcggccgg ccctcgcgct tgaagtagcc gcccggaatg cggccagccg
     5041 cgaaggcctt ctcctggtag ttgacggtga ggggcaggaa gtcgatgccc ggcttcggct
     5101 ccttggccga gacaacggtg gcgagcacgg tggtctcgcc gtaggaagcg agcacggcgc
     5161 cgtcggcctg gcgcgcgacg cggccggtct cgagggtcag cacgcgaccg gcccagttca
     5221 gtttctggcg gacgatatcg aacatgtgtg tgccttcatg gcttggtgtg tcggggccat
     5281 gggcaagacg gcgggcggtt cacaggggtg aggcgtcctg aacgccccgc gaaccgccgg
     5341 cgatcctgcc atggcgcggt actgatcaac gagcggcggg accgccgcga tcacgatgga
     5401 acggagcggc cagggccgct ccggtggctg aaatcagcgg cggatgccga gacgttcaat
     5461 cagggtcttg tagcgggctt cgtccttcga cttcacgtag tcgaggaggc ggcggcgctg
     5521 ggagacgagc ttgagaaggc cacgacggga atggttgtcc ttgacgtggc tcttgaaatg
     5581 ctcggtgagg ttggcgatgc gctcggaaag gatcgcgacc tgcacttccg gcgacccggt
     5641 gtcgcccttc ttggtggcat attcggaaac cagcgcgttc ttgcgctcgg cagtgatcga
     5701 catcggatac tccttgtttt cttgaggatt ggcccggccg ggatgtcgtc cagcggggtc
     5761 gaagaacgcg ggatcgccct gcgttgcgcg ggcttatgca tgaaacggca tgacttgtcc
     5821 accgcgccgg cgcgcatcca gtcgcgcatc tcgcgtgtag tcagaccaga acccgatgga
     5881 gattcccatg cgcctcgctg ctcttgccct gcttttcgcc gccgctcccg ccctggccga
     5941 ggacttcggc gcctggaaac cgctgaccaa cccgttcccc tcgaccggcg gcggcggcgt
     6001 gatgatccac gactacgacc cggttgtgtc gggaaaaacc tgcaccacgg acttttccgc
     6061 gcggtccggc caggacaggt tcgacggcga ggtggtcttc gacgcggctg aaacccaagg
     6121 cggcgtgctg tgcgccaacg gccgctggcg catgaaagac ggcagcgcca cgggcaccac
     6181 gccattccgc gtcttcatcc gcgatggcgt caagcgcggc gatccgctgt agcgcgggag
     6241 agcgccggca cctcaccccg gcagatggaa ggcccgcttc ggcaggagct ggcccttttc
     6301 cgcctccgcc agcgctacaa ggcggccgtg gcacgtgaca tagaccgtac cgagagtcgc
     6361 cggtgcgtcg cggccgcgca gaatcaccgg ctggccgttg gcgagccgcg tcgcctcatg
     6421 ttggccgacg cgaacttccg gcaggtcggc cagcgcggtc tccaccggca gcaggcagcg
     6481 gcgcagatcg tcgctttccg gcccctgttc gccagcagcg cgcagttcgt caaccgtgat
     6541 ggcgtcggtc tcgtgaaacg gaccgacacg ggtgcgccga agcgcgacga catgaccgag
     6601 gcagccgagc cggcggccga gatcgcgggc gatggcgcgc acataggtgc ccttgccgca
     6661 ctcggcggcg aagaccgcgg tgtccgcgtc cgactccacc agcttcagcg accggatgaa
     6721 caccgtccgc gcttccagaa ccacctcctc gccatcccgg gcgagatcat aggcgcgctc
     6781 gccgtcgatc ttcagggccg agaacttcgg cgggacctgg ctgatctcgc cgatgaagaa
     6841 cggcagggcg ttctcgatat cctcctgcgc cgggcggcgc tcgctggtcg ccgtcacgct
     6901 gccgtcggca tcgtcggagt cggtctcggc gccccatttc accgtgaagc gataggcttt
     6961 ttcaccatcc atgacgaagg gaaccgtctt cgtcgcttcg ccaaaggcca gcggcagcag
     7021 gcccgaggcc aacggatcga gcgtaccggc atggccggcc ttcttcgcgt tgaacaggcg
     7081 cttggcgatg gatacggcgt gggtcgaggt catgccgacg ggcttgtcga gcacgatcca
     7141 gcccgagacg tcgcggcgga ttttcttcac ctggcgcggc tgttcggccc gcggcgggcg
     7201 cggctgcatg tttggggcgt cagtcattct tcagatttcg cgggcttggc caggtcgcgc
     7261 gcgacttcgg gccgcgcgag cagggtgtcg atgtgggagg cggcgtcgaa gcggtcatca
     7321 agccggaacc gcacctccgg catcgagcgc atctcgcgca ggcgcttcgc cagcgcgccg
     7381 cgaaaggcgc gggcaagccg gttgagcgcc ttgacgacct ccgcctcgtc cttgccgccg
     7441 agcggcatga cataggcggt ggcgagcttc aggtccggcg acatgcgcac ctcgggcacg
     7501 gtgatgacgt ggccctcgat gaagggatcg tggatttcgc cacgctggag gatatccgcc
     7561 agcacatggc ggacttgctc gccgacgcgc agctggcgtt gcgaaggcag gccgggaacg
     7621 ggatcgtgtc gtttcattgt ctcaccatcc cgccgggatc ggaccggccg ggatcaaaaa
     7681 atcggggcgc accctgcggc gcgcccctga ctggctcgca aggggcaatc acagcgtccg
     7741 ctggatcgtc tccacgcgga agca
//
LOCUS       DAOMXN010000120         7697 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_4952_length_7697_cov_12.267077, whole genome shotgun sequence.
ACCESSION   DAOMXN010000120 DAOMXN010000000
VERSION     DAOMXN010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 7697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7697
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4952_length_7697_cov_12.267077"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..1276)
                     /locus_tag="PK812_09060"
     CDS             complement(140..1276)
                     /locus_tag="PK812_09060"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004446453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HRJ69745.1"
                     /translation="MRIVVLDESKSGEPRVAVTPETVKKYAGLGASVTVQSGAGMLSG
                     IPDSDFEAAGATVAKDMKGALAEADILLSVGRPEKAKVKGIKTGALALAVMNPYGAEA
                     ELKPLAEAGLSCFAMELMPRITRAQVMDVLSSQANLAGYRAVVDGAAEFGRAIPMMMT
                     AAGTVPAARIFIMGVGVAGLQAIATARRMGGVVTATDVRPATKEQVESLGAKFLAVED
                     DEFKQAQTAGGYAKEMSKDYQAKQAALTASHIAKQDIVITTALIPGRPAPRLITKEMV
                     ATMRPGSVIVDLAVERGGNCELAKAGEVVVTDNGVKIVGHGNLATRLAPTSSSLYARN
                     LFAFLETMVDKKEKKLAVNWDDELVKATLLTREGSLVHAMFQPK"
     gene            complement(1331..1537)
                     /locus_tag="PK812_09065"
     CDS             complement(1331..1537)
                     /locus_tag="PK812_09065"
                     /inference="COORDINATES: protein motif:HMM:NF019452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aa3-type cytochrome c oxidase subunit IV"
                     /protein_id="HRJ69746.1"
                     /translation="MADQHAPHNVDNDYVEHEKTYELFLTLTKWGTIATVILVLFIGS
                     MTSLVPWALTLFLTVAILAASRLF"
     gene            complement(1673..3034)
                     /locus_tag="PK812_09070"
     CDS             complement(1673..3034)
                     /locus_tag="PK812_09070"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HRJ69747.1"
                     /translation="MTDDPEQNRLTARAARYAKVGANIGGVAARMAGARLTGSQNLSA
                     GNAAALAAALGRLKGPLVKVAQLMATIPDVLPADYAAELQKLQSQAPPMGWVFVKRRM
                     SAELGPDWESRFASFEKTAAAAASLGQVHRAVTHGGETVACKLQYPDMASVVEADLQQ
                     LSILFALHRRMNGVIDTSQAAEEIAARVREELDYVREAGHARLYGQMLKDTALVRVPA
                     VHASLSTRRLITLGWLDGRRLLDFKGHDLEDRNRLATAMFTAWWLPFSHYGVIHGDPH
                     LGNYTVFEQAGRPAGINLLDYGCIRIFPPRFVGGVVDLYEGLRTDNRERIVHAYETWG
                     FRSLNAGLIDTLNIWARFIYGPLLDDRVRTIADSVSPAEYGRREAGRVQQELRKHGAV
                     TVPREFVFMDRAAIGLGGVFLHLGAELNFYRLFNDQIADFSLERVAARQTAALAEAGL
                     SSG"
     gene            3115..3393
                     /locus_tag="PK812_09075"
     CDS             3115..3393
                     /locus_tag="PK812_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69748.1"
                     /translation="MSESPSPSRPTNALLALGAALTVFLVLVAIERSARSCGDGLCGS
                     ISGAMAILGLMGASLLFTLRGLKRGEAPRWLFVIPWIVWALALWILLF"
     gene            complement(3421..4296)
                     /locus_tag="PK812_09080"
     CDS             complement(3421..4296)
                     /locus_tag="PK812_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008191778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRJ69749.1"
                     /translation="MQARANLSGILWMVTAMAAFAIEDALIKHCARQLPFGQVLVLFG
                     LAGAGIFAALVRLAGGTLFRRDVLSRLMLVRAVFELAGRLFYFLAVALTSLSSATAIL
                     QATPVLVVLGGGVFFGERVGWRQWCAVGAGLVGVLIVLRPAAGDFSVLSVLTVIGIIG
                     FAGRDLASRAAPASLSAPHLGFYGFLTVVIAGTLYSLWDARVFVWPDAISAASLAGAV
                     AFGVLAYGALMKAMRTGDIATVTPFRYTRLLFGLGLGMLFFGERIDAPMIIGCACIIS
                     AGGLIAWHRHATRKG"
     gene            complement(4337..>4861)
                     /locus_tag="PK812_09085"
     CDS             complement(4337..>4861)
                     /locus_tag="PK812_09085"
                     /inference="COORDINATES: protein motif:HMM:NF023354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3445 domain-containing protein"
                     /protein_id="HRJ69750.1"
                     /translation="ASLCFPSAWSLREKFGLPMADIHGGVPGYPGRMEQLIARMFDNL
                     RVENPVSRQNWSIYGDARLHHPESKSDPHERFPPDSPVHDRAIIRVERQTLRRLPTSD
                     AILFTIRVHLAPMAALKDHPRGGVLAASLRDQLLALTAEQLAYKGLVYARERLAEALA
                     VIAGAPQMPTRPPT"
     assembly_gap    4862..4961
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5040..6869)
                     /locus_tag="PK812_09090"
     CDS             complement(5040..6869)
                     /locus_tag="PK812_09090"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012454752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HRJ69751.1"
                     /translation="MSRISVLETPSGANKRAEQDGLPEWNLTHLYQGMDSAHYRADLE
                     SAEPDCRRFAEGLRGRIATLAAASDGAERLFEAVCEYEKIEDKLGRIMSFAGLVYAGD
                     TTDPARGKFYGDAQEKITAASSELLFFQLEINRLDDALFAKLASTAPLVRYRPWLEDI
                     RKEKPYQLDDRLEQLFHEKSVTGRSAWNRLFDETIASLRFDVDGKTLTLEPTLNLMQD
                     RDEAKRKAGAAALSKTFGENLRVFTLITNTLAKDKEIGDRWRGFEDVADARHLSNRVE
                     REVVDALVSAVQAAYPRLSHRYYALKAKWFGKDKLEFWDRNAPLPSVEQRHIPWNEAR
                     DTVLSAYSDFAPEMARIARRFFDESWIDAPVRPGKAPGAFAHPTVPSAHPYVLVNYQG
                     KPRDVMTLAHELGHGVHQVLAAPNGALMAPTPLTLAETASVFGEMLTFRKVLAGTTDP
                     AQKKALLAGKVEDMINTVVRQIAFYSFERKVHTERKNGELTADKLNEIWMSVQGDSLG
                     PSIRLSAGYEVYWSYIPHFIHSPFYVYAYAFGDCLVNSLYGVYQKADPKFVQNYFALL
                     SAGGTKHHSELLAPFGLDARDPAFWQIGLGMIEGMIAELEAME"
     gene            7039..>7697
                     /locus_tag="PK812_09095"
     CDS             7039..>7697
                     /locus_tag="PK812_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012454751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRJ69752.1"
                     /translation="MTASILIVDDDPVQRRLLTEMVRRFGFDPVAVEGGAQALEKLQS
                     GVEFGAVVLDLVMPDLDGMGVLSRMKDMDGAPPVIVQTAHGSIETVISAMRAGAIDFV
                     VKPVGAERLQVSIRNALRVDTLQGEVRRLTKRATGGLQFKDLGASSSDMIRAVRLGER
                     AAKSGIPVLLEGESGVGKEVFARAVQGASDRRGKAFVTVNCGAIPANLVESILFGHEK
                     GA"
BASE COUNT         1358 a         2385 c         2491 g         1363 t
ORIGIN      
        1 ggtcgccttg gtcagcgcct ccacggcgcc ctgcgcctgc tgcgcagcct tttgcgcggc
       61 ggcggccgca gccttggcct gttcgagaag ttcggcggga gtagccatgt ccgcagttcc
      121 ttgagtgctt ctctgagggt tacttcggct ggaacatcgc gtggaccagc gagccctcgc
      181 gcgtgagcag tgtcgccttc accaactcgt cgtcccaatt caccgcgagc ttcttttcct
      241 tcttgtcgac catggtctca aggaaggcga agaggttgcg ggcgtagagg ctggaggagg
      301 tgggcgcgag gcgggtcgcc agattgccgt ggccgacgat cttcacgccg ttgtccgtca
      361 cgacgacttc accggccttc gccagttcgc aattgccgcc gcgttcgacc gcgaggtcga
      421 cgatcaccga gccaggccgc atggtcgcga ccatctcctt ggtgatcagg cgcggtgcag
      481 gccgccccgg aatcagtgcc gtggtgatga cgatgtcctg cttggcgatg tggctcgccg
      541 tgagcgccgc ctgcttggcc tggtagtcct tgctcatttc cttggcgtag ccgccggcgg
      601 tctgcgcctg cttgaactca tcatcctcga cggcaaggaa cttcgcgccg agcgactcca
      661 cctgttcctt ggtggccggg cgcacgtcgg tcgcggtcac cacgccgccc atgcggcggg
      721 ccgtggcgat ggcctgcaag ccggcgacgc ccacgcccat gatgaagatc ctcgccgccg
      781 gaaccgtgcc ggccgccgtc atcatcatcg gaatggcgcg tccgaattcg gcagccccgt
      841 ccacaacggc gcgatagccc gcaaggttcg cttgcgatga caggacgtcc atcacctgag
      901 cgcgcgtgat gcgcggcatc agctccatgg cgaagcagga gagcccggct tccgccaacg
      961 gcttcaattc ggcttccgcg ccgtaggggt tcatcacggc cagcgcgagc gcgccggtct
     1021 tgatgccctt gacctttgct ttctccgggc gaccgaccga cagcaggatg tcggcctccg
     1081 ccagcgcgcc cttcatgtcc ttggcgacgg tcgcgcccgc cgcctcgaag tcgctgtcgg
     1141 gaatgccgga gagcataccg gctccggact ggaccgtcac ggaagcgccc agtccggcgt
     1201 acttcttcac ggtttccggc gtgacggcca cgcgcggttc gccgcttttc gattcatcga
     1261 gaaccacgat gcgcatcggc acaacctcag gacggacgaa cgggacggcg gcgtggtcga
     1321 agccgctgga tcagaacagc cgcgaagcgg cgagaatcgc gacagtcagg aacagcgtca
     1381 acgcccatgg cacgaggctg gtcatggagc cgatgaagag gacaagaatc accgtggcga
     1441 tggttcccca cttggtgagc gtcagaaaca gctcataagt cttttcatgc tcgacatagt
     1501 cgttgtcgac attatgcggc gcgtgctgat cagccatgga aacccctcga aggcgagatg
     1561 caattgttga ccaaatagcc tgtagcggat gccagaaaaa gccgcaattg gcggaggatc
     1621 ggaattttgc cgggctgatc accggatcgc cgcagcatag tgccgcaggc ggtcacccgg
     1681 atgacaggcc cgcttcggcc aatgctgcgg tctggcgtgc cgcgaccctt tccagggaga
     1741 aatcggcgat ctggtcattg aagagccgat agaagttcag ctcagcgcca aggtgcagga
     1801 aaacgccgcc gaggccgatc gcggcacggt ccatgaagac gaactcgcgc ggaaccgtca
     1861 cagcgccgtg cttgcgcagt tcctgctgaa cgcgccctgc ctcgcggcgt ccatattctg
     1921 caggactgac gctgtcggcg atggtgcgca cgcggtcatc gagaagagga ccgtagatga
     1981 accgcgccca gatgttcagt gtgtcgatca gcccggcatt gaggctcctg aagccccagg
     2041 tctcgtaggc atgtacgatc cgctcgcggt tgtcggtgcg caggccttcg taaagatcca
     2101 ccacgccgcc gacgaagcgc ggtgggaaga tgcggatgca gccgtagtcc agcagattga
     2161 tgccggctgg cctgccggcc tgctcgaaca ccgtgtagtt gccgaggtgt gggtccccat
     2221 gaatgacacc gtagtggctg aacggcagcc accaggcggt gaacatggcg gtcgccagcc
     2281 ggttgcgatc ttcgagatca tggcccttga agtcgagcaa ccggcgcccg tccagccagc
     2341 cgagggtgat cagcctgcgc gtcgacaggc tggcgtggac cgctggaacg cgcacgaggg
     2401 cggtgtcctt cagcatctgg ccatagaggc gcgcgtgtcc cgcctctcgc acgtagtcca
     2461 gttcttcgcg cacgcgtgcc gcgatttctt cagccgcctg cgatgtatcg atgacgccgt
     2521 tcatgcggcg gtggagcgcg aacaggatgc tcagttgctg gaggtccgcc tcgacaaccg
     2581 aggccatgtc cgggtactgc agcttgcagg cgaccgtctc gcccccgtgc gtcaccgcgc
     2641 ggtgcacctg gccgagggac gcggctgccg ccgccgtctt ctcgaaagag gcaaaacggc
     2701 tttcccaatc aggtcccagt tccgcgctca tgcggcgctt gacgaatacc cagcccatgg
     2761 gcggggcctg cgactgcagc ttctggagtt cggcagcata gtcggccggc aacacgtccg
     2821 gtatcgtcgc catcaactgg gcgaccttga ccagcggacc cttgaggcgc ccgagggcgg
     2881 ccgccagcgc cgccgcattg cctgcggaca gattctgcga ccccgtcagt cgggcgccgg
     2941 ccatgcgcgc ggccacgccg ccgatattgg cgccgacctt ggcatagcgc gccgcgcggg
     3001 cggtgaggcg gttctgttcg ggatcgtcgg tcacaggagg aagataggca gcgctggccg
     3061 tttcagctac tgttcgctgc aggaaatcgt tggttcgtgg agggtgagac cagcatgtcc
     3121 gaaagcccaa gcccgtcgcg accaaccaat gccctgctgg cgctcggcgc cgcgctcacg
     3181 gtttttctgg tgcttgttgc gatcgagcga tctgcgcgct cctgcggcga cgggctttgc
     3241 gggtctatct cgggcgccat ggcgatcctt ggtctcatgg gggcgtccct gttgttcacg
     3301 ctccggggcc tcaaacgggg agaagcgccg cgctggttgt tcgtcatccc ctggatagtc
     3361 tgggcccttg cgctttggat tctcctgttc tgacgggccc gggaggctat ggatcaggct
     3421 tcagcctttg cgtgttgcat ggcggtgcca tgcgatcagc ccgcccgccg atatgatgca
     3481 agcgcagcca ataatcatcg gtgcgtcaat cctttcgccg aaaaacagca tgccgaggcc
     3541 gaggccgaaa agcagtcgcg tgtagcgaaa gggcgtgacg gtcgcgatat cgccggtccg
     3601 catggctttc atcaacgcgc cataggccag aaccccgaat gctaccgccc cggcgaggct
     3661 cgcggcagat atggcgtccg gccagacgaa gacccgggcg tcccagaggg aatagagcgt
     3721 gccggcgatg accaccgtga gaaagccata gaagccgagg tgtggcgcgc tcagcgatgc
     3781 cggagcggcg cggctcgcca gatcgcggcc ggcaaagccg atgatgccga tcaccgtgag
     3841 gaccgacagc accgagaagt caccggcggc cgggcgcagg acgatcagca ctccgaccag
     3901 tcctgcgccc acggcgcacc actgtcgcca gccgactctt tcgccaaaga acacaccgcc
     3961 ccccagcacg acaagcaccg gcgttgcttg aaggatggcg gtcgccgagg acagcgaggt
     4021 caatgcaacg gcgagaaagt agaacagccg gccggccagc tcgaaaacgg cgcggaccaa
     4081 catcaatcgt gacagcacgt cgcggcgaaa cagggttcct ccggccagac ggaccaacgc
     4141 ggcgaaaatg ccggcgccgg caaggccgaa aagcacgagc acctggccga acggcagctg
     4201 tcgcgcgcag tgcttgatca acgcgtcctc tatggcgaac gccgccatgg ctgtcaccat
     4261 ccacaggatg ccggacaggt tcgccctagc ctgcacggca gtggtccgtt gtgctgggat
     4321 cagcaggcat ggtgcatcag gttggggggc gggtcggcat ttggggggca ccggcgatca
     4381 cagccagcgc ttccgccagg cgctctcggg cgtagaccag gcccttgtag gcgagttgct
     4441 ctgccgtgag agcaagcagc tgatcacgca gcgaggccgc cagcacgccg cctcgcggat
     4501 ggtccttgag ggcagccatc ggcgccaggt gcacgcggat ggtgaacagg atggcgtcgc
     4561 tggtcggcag gcggcggagc gtctggcgct cgacccggat gatggcgcgg tcatgcaccg
     4621 gggagtccgg cggaaagcgc tcgtgcggat cggatttcga ttcgggatgg tgcagacggg
     4681 catcgccgta gatcgaccag ttctgccggc tcacggggtt ctcgacccgg agattgtcga
     4741 acatgcgcgc gatcagctgc tccatccggc cgggatatcc cggaacgccg ccgtggatgt
     4801 ccgccatggg caggccgaat ttttccctga gcgaccacgc tgagggaaag cacagggaag
     4861 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgagatcaa gcggctccag
     4981 acctatggaa aacggccttt tcgatccgtc gtagggcgta tgggcgaagc cggacacact
     5041 cattccatcg cttcgagttc ggcgatcatg ccttcgatca tgccgaggcc gatctgccag
     5101 aacgccgggt cgcgcgcgtc gaggccgaac ggcgccagca actccgaatg atgctttgtg
     5161 ccgccggcgg agagcagcgc gaagtagttc tgcacaaact tcggatcggc cttctggtag
     5221 acgccgtaga gcgaattcac caggcaatcg ccaaacgcgt aggcatagac atagaacggc
     5281 gaatggatga agtgcgggat gtaggaccag tatacttcgt aacctgcgct gagccggatc
     5341 gacgggccga ggctgtcgcc ctgcacgctc atccaaatct cgttgagctt gtccgcggtg
     5401 agttcgccgt tcttgcgctc cgtgtgaacc ttgcgctcga aggagtagaa ggcgatctgc
     5461 cgaaccacgg tgttgatcat gtcctcgacc ttgccggcga gcagggcctt cttctgcgcc
     5521 gggtcggtgg tgccggccag caccttgcgg aaggtcagca tctcgccgaa caccgaggcg
     5581 gtctcagcca aggtcagcgg cgtcggcgcc atcagcgcgc cgttcggcgc ggcaagcacc
     5641 tgatgcacgc catgcccgag ctcatgggcc agggtcatca catcgcgcgg ctttccctga
     5701 tagttcacca gaacataggg gtgtgctgag ggcactgtcg ggtgcgcgaa ggcgcctggt
     5761 gccttgccgg ggcgcactgg cgcatcgatc cagctctcgt cgaagaaacg ccgggcgatc
     5821 cgcgccattt cgggtgcgaa atccgaatag gcggagagaa ccgtatcccg cgcctcgttc
     5881 cagggaatgt ggcgctgttc gacggacggc aggggagcat tgcgatccca gaattcgagc
     5941 ttgtccttgc cgaaccactt cgccttcaga gcgtagtagc ggtgcgacag gcgcggatag
     6001 gcggcctgca cggcggaaac gagcgcatcg acgacttccc gctcgacgcg gttcgacagg
     6061 tggcgggcgt ccgcgacgtc ctcgaatcca cgccagcggt cgccgatctc cttgtccttg
     6121 gcgagcgtgt tggtgatcag cgtgaagacg cgcaggttct cgccaaaggt cttcgaaaga
     6181 gcggccgcac ccgccttgcg cttggcttcg tcccgatcct gcatcaggtt gagcgtcggt
     6241 tcgagtgtca gcgtcttacc gtcgacatcg aagcggagcg aggcgatggt ctcgtcgaac
     6301 agccggttcc aggcggaccg gccggtcact gatttctcgt ggaacaactg ctccagcctg
     6361 tcgtcgagct ggtacggctt ttccttgcgg atgtcttcaa gccagggccg gtaacgcacg
     6421 agcggtgccg ttgaggccag tttcgcgaac agcgcatcat caagccggtt gatctcgagc
     6481 tggaagaaga ggagctcgga cgaggccgcc gtgatcttct cctgcgcgtc gccgtagaat
     6541 tttccgcggg caggatcggt ggtgtcaccg gcatagacca atccggcgaa ggacatgatg
     6601 cgacccagct tgtcctcgat cttctcgtac tcgcataccg cctcgaacag acgctcggca
     6661 ccgtcgctgg ccgcggcaag agtggcgatc cggccgcgca ggccttcagc gaatcggcgg
     6721 cagtccggtt cggccgattc gaggtccgcg cgatagtgag ccgaatccat gccctgatag
     6781 agatgggtca ggttccattc cggaagccca tcctgctccg cgcgcttgtt cgcgcccgaa
     6841 ggcgtctcca gaaccgaaat cctgctcatt tcccgcaact ctccgcaaaa tcgcatcgac
     6901 tccatatcgc ccgcagggtc tcttcttcaa gcccgacagc ccgatcgtga tcatgattta
     6961 cagcctgttt acgcaactgc cgcacagtgc atcaaaagga gacagcgctt ggctgtcgcc
     7021 gaagtaggga atgacggaat gacggctagc atcttgatcg tcgatgatga tccggtccag
     7081 cgcaggctgc tgaccgagat ggtccggcgg ttcggcttcg atccggttgc ggtcgaaggg
     7141 ggcgctcagg cattggagaa actccagtcg ggcgtggaat tcggcgccgt tgttcttgac
     7201 cttgtgatgc cggacctcga tggcatgggc gtgctttcgc gcatgaagga catggacggg
     7261 gcgccgccgg tcatcgtcca gactgcccat ggctccatcg agaccgtcat atccgcgatg
     7321 cgcgccgggg cgatcgactt tgtcgtcaag ccggttggcg cggaacgcct ccaggtttca
     7381 atacgcaacg cgctgcgggt cgacaccctt cagggcgaag tgcgccggct gaccaaacgg
     7441 gccaccggcg gcctgcaatt caaggatctt ggcgccagtt cgtccgacat gatccgtgcg
     7501 gtgcgccttg gcgagcgggc ggcgaaatcc ggcattccgg tgctgctcga aggtgaatcc
     7561 ggcgtcggca aggaagtgtt tgcccgcgcc gtacagggcg cgagcgaccg gcgcggcaag
     7621 gcgttcgtca ccgtcaattg tggcgccatt ccggccaatc tcgtggaaag catcctgttc
     7681 ggtcacgaga agggcgc
//
LOCUS       DAOMXN010000121         7691 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_4958_length_7691_cov_9.669199, whole genome shotgun sequence.
ACCESSION   DAOMXN010000121 DAOMXN010000000
VERSION     DAOMXN010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 7691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7691
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4958_length_7691_cov_9.669199"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="PK812_09100"
     CDS             <1..655
                     /locus_tag="PK812_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007758872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HRJ69753.1"
                     /translation="CSACHGLGLVAFRNLSDKGGPEFSVAQVKELASTYKVQDGPNDK
                     GEMFERPGRPADKLPTPFANAEAAKAAHGAVPPDLSLIAKARSYERGVLLSLVDLVSQ
                     YQEHGPDYLVALLTGYTDPPKGVTVEQGLHYNPYFAAGDKLAMASPLNPDQVEYTDGT
                     KGTVQQYAKDVTAFLMWAAEPKMEERKRTGWFFMILMVLLAGFTYFTKKKIWSDVAH"
     gene            906..1232
                     /locus_tag="PK812_09105"
     CDS             906..1232
                     /locus_tag="PK812_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69754.1"
                     /translation="MSSVQFAERLKTHAMEHAAVDFEKMHEVAKHLHETPGAVDEFCK
                     DPEGYVFRFNGLKVQDGHHLHVADAENNLLPPEKYGVFGSEDRPKWGRSEIRVGYKTT
                     ALVECV"
     gene            complement(1300..3078)
                     /locus_tag="PK812_09110"
     CDS             complement(1300..3078)
                     /locus_tag="PK812_09110"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRJ69755.1"
                     /translation="MSLSRFLAARRLPETQPPAAAVAESTEAPPVSYLFEGRPLKRTP
                     IELAPIVDELEHDVALTLQLISDITEETRERVAENVALAGQIQTSSRGLANLSASARD
                     TSERLAETARQIESSNREIERQVIDSDDVLKEARGLAGTVNAQMQGLSQVARNIAVVV
                     EAIRTIARQTNLLALNATIEAARAGAAGRGFAVVATEVKTLSGQVQAATADISRQIGA
                     LEAAVNGSTATMTRMADLIGRFDPVLGSIRDAAGIQIEGTRAVATRSVATADFADAVA
                     QSAEAMHDLAGSAAEVAGRAGLAVQGATLTLERINKRSMLYFRHSIIEERRETERLPV
                     LINGFFRSRDHAGEVLAIDLGANGVQLERSAFSPAEGSIGLLTLDGIGASEARVVETN
                     EFGLNVRFERPSAEFLASVAQRIERVHEECAPYLTRLATVAREVEGVLEEGVASGAVT
                     LEALLQPKYQKVPDTNPIQYVTAATPFYDRFFAPLVGRHRNTVPGAVFLVPIDRNSYL
                     PVHYPEYSQTQRSGDLAWNDLNARNRRVMPRAQTLRAARNEMQGKLQLYVRDMRDGRR
                     IYGRLIAFPVRLRDRTWGNIICCLPM"
     gene            3226..3987
                     /locus_tag="PK812_09115"
     CDS             3226..3987
                     /locus_tag="PK812_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HRJ69756.1"
                     /translation="MAEVLWNRLTAAELVALAKKDAIVLLPVASTEQHGPHLATGVDT
                     FLAGEGCRRTALLVQRKRPIVVAPTVWIGLAEHHMAFGGSFTLSLPTYHALLREICRS
                     ILRAGFRKILIVNGHGGNMAALGALTTDLAMELGAPIATTTLYALPTAAGAYEGILED
                     QKGVQHACEAETSMMMAAFPDCVRKNKLKAAVGPKSDDAPFRSAVAVWTSFKELTPSG
                     VFGDGRRATPEKGEKLLDIAAQLLAQHLLTGKPWA"
     gene            3984..4859
                     /locus_tag="PK812_09120"
     CDS             3984..4859
                     /locus_tag="PK812_09120"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012317473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HRJ69757.1"
                     /translation="MSKAVLGVIGGSGVYDLPGLENITEERIASPWGEPSDAVRRGTL
                     GGTDLVFLPRHGRGHRLSPSDINYRANIDVLKRAGVTDLVALSACGSFREDLPPGTFL
                     LVDQFVDRTHRRDSSFFGKGCVAHVTMAHPVGPRLQDMIAEAGVAAGVDVRRGGTYVC
                     MEGPQFSSLAESRTYKGLGYDVVGMTAMPEVKLAREAEITYATIAMVTDFDCWHPDHD
                     HVSVADVVRVMHDNVGRARALLAALARAFPKERVPCPVGSHVALDHAVMTAKDARDPA
                     LLRKLDAVAGRVLNQ"
     gene            4878..5402
                     /locus_tag="PK812_09125"
     CDS             4878..5402
                     /locus_tag="PK812_09125"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HRJ69758.1"
                     /translation="MIKDAIRTIPDYPKPGIMFRDITTLLNNPRAFRKAVDEMVQAFA
                     GTKIDHVVGIEARGFILGGAVAHQLSAGFIPVRKKGKLPHETVRIAYSLEYGIDEMEM
                     HKDAIKPGERVILIDDLIATGGTAEGAVKLLRSLGATVEAACFVIDLPDLGGAKKLQA
                     MGVTVLSLIAFEGH"
     gene            5590..5928
                     /locus_tag="PK812_09130"
     CDS             5590..5928
                     /locus_tag="PK812_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69759.1"
                     /translation="MAFFLYDSLKEKIQLMNQNQKRSMKGTGVYSVPAPDKGASGRRT
                     ILVPNLTYDEAYGRSIHCIEATWIPESEMKFEDMSADEKYCGQPCSYSGQCNFSSNCF
                     FCVPFDGCGT"
     gene            complement(6058..6573)
                     /locus_tag="PK812_09135"
     CDS             complement(6058..6573)
                     /locus_tag="PK812_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69760.1"
                     /translation="MKLVIAVLNLINPADRFMDITWKVSTYGDGTQYLYFIRVPPPCT
                     EFAPIIPPPDPERFYLPSFGQGPCINSKLAPKIGPTINVDSGENLDLVFEIAGWPDYP
                     LHPGEPHGIIVKLGNEAKKAARGETSITFTNVLGDKLELEIAIEGAAFLGNTPLTRSC
                     PTLLPLIVSRT"
     gene            6970..7446
                     /locus_tag="PK812_09140"
     CDS             6970..7446
                     /locus_tag="PK812_09140"
                     /inference="COORDINATES: protein motif:HMM:NF019023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1493 family protein"
                     /protein_id="HRJ69761.1"
                     /translation="MLRLCSVATPGSQREVDLRKFLCRRYWLPESDFPLCGDIFRALG
                     LTGDDCHDFMNDLAETFNLDLADFVWPKFHLGESEALDVRAALRPLRRFAGIRTQPLD
                     RDLIPISIDHLSQVIEHGCWFDPQQPDMAASASVSQTISRLSAKLRAGGVPRRSLP"
     gene            complement(7409..>7691)
                     /locus_tag="PK812_09145"
     CDS             complement(7409..>7691)
                     /locus_tag="PK812_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HRJ69762.1"
                     /translation="GCLARRRAAMLEAGAVDGRVARLGPSPGIENLRWLRPTREGDTI
                     RYYSQVMDKRESASRPGWGIVTSQGSGYLASGERVFELTAAIFWERRPP"
BASE COUNT         1472 a         2455 c         2362 g         1402 t
ORIGIN      
        1 ctgctcggcc tgccacggcc ttggcctggt cgccttccgc aacctctcgg acaagggcgg
       61 cccggaattc tccgtcgccc aggtcaagga actggcctcc acctacaagg tgcaggacgg
      121 accgaacgac aagggcgaga tgttcgagcg gcccggccgc ccggccgaca agctgccgac
      181 gcccttcgcc aacgcggaag cggccaaggc cgcccacggc gcggtgccgc cggacctgtc
      241 gctgatcgcc aaggcgcgca gctatgagcg cggcgtgctg ctgtccctgg tcgatctcgt
      301 gagccagtac caggagcacg gcccggacta cctcgtggcg ctgctcaccg gctataccga
      361 tccgccgaag ggcgtgacgg tggaacaggg actgcactac aacccctact tcgccgccgg
      421 cgacaagctg gccatggcca gcccgctcaa tccggatcag gtggaataca ccgacggcac
      481 caagggcacg gtccagcaat acgccaagga tgtcaccgcc ttcctgatgt gggcggccga
      541 gcccaagatg gaagagcgca agcgcaccgg ctggttcttc atgatcctga tggtgctgct
      601 cgccggcttc acctacttca ccaagaagaa gatctggtcc gacgtggcgc attgacgcac
      661 ggaccggcgc tccgtcgccg gctccggtcc gaaccgatga cgaaacgggg ccctcgcggg
      721 cccctttttt gttgcgaagc gcgtttgcgc gagaggcctt cgcaagtcaa cgtcggtgtc
      781 aagcgtgcgg ctgtgaattc gtcatgacgc acggtgaacc tgcttgccgg gtttccagac
      841 ctgtgaaagg ttaacgtttg ttaaccataa tggtggcagc cgccgccaag agcaggaggc
      901 ttggaatgtc ttcagtccaa ttcgctgaac gcctgaaaac ccatgcaatg gagcatgcgg
      961 ccgtcgactt tgaaaagatg cacgaggtcg ccaagcatct ccatgagacg ccaggcgccg
     1021 tcgacgagtt ctgcaaggat ccggaaggct acgtcttccg cttcaacggc ctgaaagtgc
     1081 aggacggaca tcacctgcat gtcgccgatg ccgagaacaa cctgcttcca cccgagaaat
     1141 acggcgtgtt cggctccgaa gaccgcccga aatggggccg gtcggaaatc cgcgtcggct
     1201 acaagaccac cgctctggtc gagtgcgtct gaaccgacgc ggcgaccgcg acagtcccgt
     1261 cgatccgcca atggcaaggc gcccttcggg gcgcctttgt cacatcggca ggcagcagat
     1321 gatgttgccc caagtccggt cacgcaggcg aaccggaaag gcaatcaggc gtccgtagat
     1381 gcggcggccg tcgcgcatgt cgcgcacata gagttgtagc ttgccctgca tttcgtttcg
     1441 ggcggcgcgc agagtctggg cgcggggcat gacgcgacgg ttgcgcgcat tcagatcgtt
     1501 ccaggcgagg tcgccggaac gttgcgtctg cgagtactcg ggatagtgca ccggaaggta
     1561 ggaattccgg tcgatcggca ccaggaacac cgccccaggc accgtgttcc tgtgcctgcc
     1621 gaccagcggc gcaaaaaagc ggtcgtagaa cggcgtcgcc gccgtgacgt actggatggg
     1681 attggtatcg ggaacctttt ggtatttcgg ctgaagcagc gcttccagcg tcacggcgcc
     1741 gctggcgacg ccttcctcga gcacgccctc cacttcgcgc gcgaccgtcg ccagccgcgt
     1801 gagataggga gcgcattcct catgaacgcg ctcgatgcgc tgggcgaccg aggccaagaa
     1861 ctcggccgac ggccgctcga accgcacgtt cagcccgaac tcgttggtct cgaccacccg
     1921 cgcctcgctg gcaccgatcc cgtcgagcgt cagcaacccg atgctgcctt cggccggcga
     1981 aaacgcggaa cgctccagtt gcacgccatt cgcgccgagg tcgatcgcca acacctcgcc
     2041 cgcatgatcg cgactgcgga aaaagccatt gatcaggacc ggcaggcgct cggtctcgcg
     2101 acgctcctcg atgatcgaat gccggaagta cagcatcgaa cgcttgttga tccgctccag
     2161 ggtcaacgtt gccccctgca cggccagtcc cgcccgccca gccacttccg cagccgaccc
     2221 ggcaaggtcg tgcatggcct ccgcgctttg ggcgacggca tcggcgaagt cagccgtcgc
     2281 caccgaccgc gtggccacgg cgcgcgttcc ctcgatctgg ataccagcgg cgtcgcggat
     2341 cgatccgagc accgggtcga agcggccgat caggtctgcc atgcgcgtca tggtcgccgt
     2401 cgaaccgttc accgccgctt ccagcgctcc gatctggcgg ctgatgtccg ccgtggcagc
     2461 ctgcacctgg ccggacaggg tcttcacttc cgtcgcgacc accgcgaatc cgcggccggc
     2521 ggcaccggcg cgcgcggcct cgatcgtggc attgagcgca agcaggttcg tctggcgggc
     2581 gatggtgcgg atggcctcga cgacgacggc aatgttccgc gccacctgcg acaggccctg
     2641 catctgggca ttgaccgtgc cggcaaggcc gcgcgcttcc ttcagcacat catccgaatc
     2701 gatgacctgc cgctcgatct cccgattgga ggattcgatc tgccgggcgg tctccgccag
     2761 ccgttcggac gtatcgcgcg cggaggccga gaggttggcg aggccccggc tggaagtctg
     2821 gatctggccg gccagcgcca cgttctcggc aacgcgttcg cgcgtctcct cggtgatgtc
     2881 gctgatcagc tggagggtga gggccacatc atgctcaagc tcgtcgacga tcggcgcaag
     2941 ctcgatgggc gtgcgcttca ggggccgtcc ctcgaagaga tacgagaccg ggggagcctc
     3001 cgtgctttcg gcaacggctg ccgccggagg ctgggtctcg ggcaggcgtc gggcggcgag
     3061 gaaacgggac agcgacacgg ggacggcctt ctgcgcaatc ggttcgggct atcgaaccat
     3121 agagcgccgc aaaggcttta ggtccggtta accacgattg ccggcggccg cacttgcccc
     3181 gccggtgaga agccgcgtaa ccttcctgaa aaccgggagc gcgcaatggc ggaagtactt
     3241 tggaatcgat tgacggcggc cgaactcgtg gcgctggcga agaaggatgc gatcgtgctg
     3301 ttgccggtcg cctcgaccga gcagcacggt ccgcatctgg cgacgggcgt cgacacattc
     3361 ctcgccggcg aaggctgccg ccgcaccgcc ctgctggtgc agaggaagcg gccaatcgtc
     3421 gtggcgccca cggtctggat cgggcttgcg gaacatcaca tggccttcgg cggctcattc
     3481 acgctgtcgc tgccgaccta tcacgcgctg ctgcgcgaga tctgccgctc gatcctgcgc
     3541 gccggattca ggaagatcct catcgtcaac ggccatggcg gcaacatggc cgcgctcggc
     3601 gcgctgacca cggacctggc gatggagctg ggcgcgccca tcgccaccac gacactctac
     3661 gccctgccga ctgcggcagg ggcctacgag ggcattctcg aagaccagaa aggcgtgcag
     3721 cacgcctgcg aggccgagac ctcgatgatg atggcggcgt ttcccgattg cgtgcgcaag
     3781 aacaagctca aggcggctgt cggccccaag tcggatgacg cgccgtttcg cagcgcggta
     3841 gccgtctgga catccttcaa ggagctcacg ccctccggcg ttttcggcga cggccggcgc
     3901 gcaacgccgg aaaaaggcga gaagctgctc gacatcgcag cgcaactgct ggctcagcat
     3961 ctcctgaccg gaaagccgtg ggcatgagca aggccgttct cggcgtcatc ggcggctccg
     4021 gcgtctatga cctgccgggt ctcgagaaca tcaccgagga gcggatcgca agcccctggg
     4081 gcgagccttc cgacgcggtg cggcgcggca cgctcggcgg gacggacctg gtgttcctgc
     4141 cccggcacgg gcgcggacac cgcttatccc cttccgacat caactaccgc gccaacatcg
     4201 acgtgctcaa gcgcgccggc gtcaccgacc tcgtggcgct gtcggcctgc ggctcgttcc
     4261 gcgaggacct gccgccggga acctttctgc tggtggacca gttcgtcgac cgcacgcacc
     4321 gacgcgactc gtcattcttc ggcaagggct gcgtcgccca tgtcaccatg gctcatcccg
     4381 ttggtccacg gttgcaggac atgatcgccg aggccggtgt ggccgcaggt gtcgacgtgc
     4441 ggcgcggggg aacctacgtc tgcatggaag gtccgcaatt ctcctccctc gccgaatcgc
     4501 gcacctacaa gggcctcggc tacgacgtgg tcggcatgac cgccatgccg gaggtcaagc
     4561 tcgcccgcga ggccgaaatc acctacgcca ccatcgccat ggtcaccgat ttcgattgct
     4621 ggcaccccga tcacgaccat gtctcggtgg cggacgtggt gcgcgtcatg cacgacaacg
     4681 tcgggcgcgc ccgcgccctg ctcgctgcgc tggcccgcgc cttcccgaag gagcgcgtgc
     4741 cctgccccgt cgggtcgcat gtcgcgctcg accatgccgt catgaccgcg aaggacgccc
     4801 gagatcccgc cctcctcagg aaactggacg ccgtcgccgg ccgcgtcctc aatcagtgaa
     4861 taggcctgtt cgtgagcatc atcaaggacg cgatccgcac gatccccgac taccccaagc
     4921 cggggatcat gttccgggac atcaccaccc tgctcaacaa cccgcgcgcc ttccgcaagg
     4981 ccgtggacga gatggtgcag gccttcgccg gcaccaagat cgaccacgtg gtgggcatcg
     5041 aggcacgcgg cttcatcctc ggcggcgcgg tggcgcacca gctgtccgcc ggcttcatcc
     5101 cggtgcgcaa gaagggcaag ctgccgcacg agaccgtccg catcgcctat tcgctggaat
     5161 acggcatcga cgagatggag atgcacaagg acgccatcaa gccgggcgag cgcgtcatcc
     5221 tgatcgacga cctgatcgcc acggggggca cggccgaagg cgcggtcaag ctgctgcgct
     5281 ccctcggcgc aaccgttgag gccgcctgct tcgtcatcga cctgccggac ctcggcggcg
     5341 cgaaaaaact gcaggcgatg ggcgtgaccg tgctgagcct catcgccttc gaggggcatt
     5401 gagggggcga tagttccaga aatgggctta tgcgaagggg ttttgtagac gccatttaga
     5461 gatgtcaccc tttcgcccgt cagagattca cctgtcccgt cctttgaggg ccttgcaaat
     5521 gtttggagcg actatagaca ttcaaaatgt agatgataaa gttttgctaa cagtggaaag
     5581 gacgggacta tggcattttt tctgtacgat tccttaaagg aaaaaattca gctgatgaac
     5641 caaaaccaga aacgttcaat gaaaggtacc ggggtttatt ctgtccctgc accagacaag
     5701 ggcgcttcag gacggcgaac aattctcgtg ccaaatctta cctatgacga agcttatggt
     5761 cgttcaatac attgtatcga ggcaacatgg attccagaaa gcgagatgaa atttgaggat
     5821 atgagtgcag atgaaaaata ctgcggtcag ccttgcagtt attcaggaca atgcaatttt
     5881 tctagcaatt gcttcttctg tgttccattt gatggttgcg ggacatgatt gattttacac
     5941 tggatgtatt taggttctgc catgcgaaga cacccaatta agggcagaag ggacaggtcg
     6001 ctttttgcat gcagtcataa catttcgaat cgattaattt gataaaaatt ggataaatca
     6061 agtacgtgac actattaggg gcaagagagt agggcatgaa cgagtcagcg gagtattacc
     6121 caaaaaagca gcaccttcga tagcgatttc taattccagc ttgtcaccca atacattcgt
     6181 aaatgttata gatgtttcac cacgagcggc ttttttcgct tcattaccca atttcacgat
     6241 aataccgtga ggttcacctg ggtgtagtgg atagtctggc catcccgcaa tttcaaagac
     6301 caagtcaagg ttctctccgc tgtcgacatt gattgtcggc ccaatctttg gtgcgagttt
     6361 cgaattaatg cacggcccct gaccaaagct gggcaaatag aaacgttcgg gatcgggagg
     6421 cgggatgatt ggggcgaatt cggtgcaggg cggaggcacg cgaataaaat acaaatattg
     6481 tgtaccatct ccatatgtgg atactttcca agtgatatcc atgaatcgat cagcaggatt
     6541 aattagatta agtacagcaa ttacaagttt cattggattg cctcgattta atccagactg
     6601 tagtacttta ttacaggcag agcattttcg caatggcgcg tgtaaaaaat cgttagcgag
     6661 caatccggtt tgcacaaatc aagaattctg cacaccccca ccccttgatc ccccacggct
     6721 ttttccgtgc ggaaatcggg tggagaaatc attgcccggc gtgtacagag agcacagccc
     6781 tcggaaccgg ccgcgccaga cctgccggcg cggcggcacg acgccatccg ccagacctgt
     6841 ctgcgcagcg cccgcgattg cagcgcgtta acgccccgtt aaccggcctt ttagggatgg
     6901 cgttaacccg ccggtagcta accttcggct cgcgcgccag gacggggcgc ggcgtccgga
     6961 ggagagttca tgttgcgtct gtgcagcgtc gccacaccag ggtcccagcg cgaggtggac
     7021 ctcaggaagt ttctgtgccg ccggtactgg ctgccggaat cggacttccc gctctgcggc
     7081 gacatcttcc gggcgctcgg cctgaccggc gacgattgtc acgacttcat gaacgacctg
     7141 gccgagacct tcaatctcga cctcgccgat ttcgtctggc cgaaattcca cctgggcgaa
     7201 tccgaggcgc tcgacgtgcg cgccgcgctc cggccgctgc ggcggttcgc ggggatccgc
     7261 acccagccgc tcgaccgcga tctcattccg atttccatcg accacctgtc gcaggtgatc
     7321 gagcacggct gctggttcga tccgcagcaa cccgacatgg cggcgtccgc ctccgtcagc
     7381 caaaccattt cccggctgtc ggccaagctc agggcgggcg gcgttcccag aagatcgctg
     7441 ccgtaagctc gaagacgcgc tcgccgctcg ccagatagcc cgacccctgg ctggtgacga
     7501 tgccccagcc gggacgcgag gcggattcgc gcttgtccat cacctgcgaa tagtagcgga
     7561 tcgtatcgcc ctcgcgggtg ggcctgagcc agcgcaggtt ctcgatcccc ggtgacggcc
     7621 cgagccgcgc cacccgtcca tcgacggcgc cggcttccag catcgcggcg cggcggcgcg
     7681 ccaggcagcc c
//
LOCUS       DAOMXN010000122         7612 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_5038_length_7612_cov_11.835384, whole genome shotgun sequence.
ACCESSION   DAOMXN010000122 DAOMXN010000000
VERSION     DAOMXN010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 7612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7612
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5038_length_7612_cov_11.835384"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PK812_09150"
     CDS             complement(<1..186)
                     /locus_tag="PK812_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRJ69763.1"
                     /translation="MLRRSLLCAVLAAATLPAALPALGQTWPSKPITIVVPFPPGGGT
                     DAFARPLAAQLDQQLGQR"
     gene            349..1521
                     /locus_tag="PK812_09155"
     CDS             349..1521
                     /locus_tag="PK812_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRJ69764.1"
                     /translation="MTTRPDAVLDVPRPAWWSEDIAMLREMAEKFCQSEIVPHYDRFE
                     TQEKVDRSLWNAAGASGFLCASIPEAYGGAGGSYGHEAAIVEALGHTGADGFGLPLHN
                     AIVAPYILHYGSEEQKRRWLPRLASGELVGAIAMTEPGAGSDLQGVKTTARKDGNHYV
                     VSGSKTFITNGQHANLIIVVTKTDASAGARGTSLVVVETDDAPGFERGRNLDKIGIKT
                     NDTSELFFNDVRIPTANLLGPQEGQGFFQLMSQLPQERLLIANQAMAMIERALAVTIA
                     YVKERKVFGKPVLEFQNTQFKLAELKSEATMMKVFVDHCVGELIAGRLTSTTASMVKY
                     LATDLQCRVVDECLQLHGGYGYMNEYPIARMFRDARVQRIYGGTNEIMKVLIARSL"
     gene            1527..2402
                     /locus_tag="PK812_09160"
     CDS             1527..2402
                     /locus_tag="PK812_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HRJ69765.1"
                     /translation="MSNAFHSIRYEVADRIATITLDRPERLNAIDRHMPSEIEAAVAH
                     ANADDGVHVIVLQGAGKAFCAGYDLKEFAEQGGTNPGVQDMPWDPMLDFRFMYGNTQK
                     FMALWRSYKPTIAKVHGHAVAGGSDIATCCDLIVMADDARIGYPPARVWGCPSVGMWV
                     FRVGAQNAKRLLFTGDLISAADAKSMGLALEHHPAAELHDAVARLAKRISGVPKNQLM
                     MHKLMINSALENMGLTTTQQMATLFDGITRHSPEGMWWKARAEEVGFQQAVKERDSGD
                     PIASQAEKRLPGFRS"
     gene            complement(2392..3300)
                     /locus_tag="PK812_09165"
     CDS             complement(2392..3300)
                     /locus_tag="PK812_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase family protein"
                     /protein_id="HRJ69766.1"
                     /translation="MPSPVIPEAAVARILALPLWRGEPLIEPLTGGRSNESYRVESDG
                     ARYVVRFGDDYPVHHVYRDHEAMVARAAHASGLGPEVIYDAPGVLVSRFIAACTYGAH
                     DIRANAEQVIDLVRRFHRSMPERVSGRPNLFWVFHTINDYARTLAAGGSRFRDRLPAL
                     SRLAAEFHAAQLPSELIFGHNDLVPQNILDDGHRLWLIDFEYAGFSSPLFDLAGLASN
                     AGMGSEEAEALLAHYFGAAPDAALKRAFAAMQGASLLREAMWSMVSELHLAAPGVDYA
                     GYTAECLAALDTAVDGWRSRFGRLSS"
     gene            complement(3369..3539)
                     /locus_tag="PK812_09170"
     CDS             complement(3369..3539)
                     /locus_tag="PK812_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69767.1"
                     /translation="MTVNERLTVAGLLGEWDKAARRRDRAVMIALLEKVDVADAAGIV
                     DAVLADPGRYGF"
     gene            complement(3578..4690)
                     /locus_tag="PK812_09175"
     CDS             complement(3578..4690)
                     /locus_tag="PK812_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016650879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HRJ69768.1"
                     /translation="MTSRAAVAWAANTPLTIETISIAGPKPGEVLVEIMATGVCHTDA
                     YTLSGLDSEGKFPAILGHEGAGIVREVGAGVTSVKPGDHVIPLYTPECRQCKTCLSQR
                     SNLCTAIRATQGQGVMPDGTSRFSCDGGEVFHYMGCSTFSNFTVLPEIAVAKVREDAP
                     FDKICYIGCGVTTGVGAVIHTAKVWPGANVAVFGLGGIGLNVIQGARMVGADKIIGVD
                     LNPAKAEMARKFGMTHFVNPDEIGRDKVVQAVVDLTGGGADFSFECIGNTTTMRQALE
                     CCHRGWGESIIIGVAESGKEIATRPFQLVTGRVWKGTAFGGARGRTDVPKIVDWYMEG
                     KINIDDLITHTMPLNEINTAFDLMHAGKSIRSVVVF"
     gene            complement(4745..5917)
                     /gene="pobA"
                     /locus_tag="PK812_09180"
     CDS             complement(4745..5917)
                     /gene="pobA"
                     /locus_tag="PK812_09180"
                     /EC_number="1.14.13.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018659 - 4-hydroxybenzoate
                     3-monooxygenase activity [Evidence IEA];
                     GO_process: GO:0018874 - benzoate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HRJ69769.1"
                     /translation="MRTQVAIIGSGPSGLLLGQLLSRAGISNVIVDNKPKEYILGRIR
                     AGVLEQGAAAMLEEAGVAARMHREGLVHEGFDIVFDGERHRIDLAAHSGGKTVMVYGQ
                     TEITRDLMEARQADGTPTFYAAENVAIHDIDRSSPCVTFWHEGRPYDIKCDFIAGCDG
                     YHGICRASVPAGAITRFERVYPFGWLGLLADVPPCHHELIYVHNERGFALCSQRSLTR
                     SRYYLQCSLDDTVDAWSDDLFWAELKRRLGPEIAATVQTGPSIEKSIAPLRSFVAEPL
                     RFGTLFLAGDAGHIVPPTGAKGLNLAASDVRYLFDGFVDYYRSSSQSGLDAYSQRALA
                     RIWKAERFSWWMTNLLHTFPQVSGFDRRMQRTDFAYLISSEAAQRSLAENYVGLPY"
     gene            complement(6065..6295)
                     /gene="rpmE"
                     /locus_tag="PK812_09185"
     CDS             complement(6065..6295)
                     /gene="rpmE"
                     /locus_tag="PK812_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HRJ69770.1"
                     /translation="MKAETHPDYHFIKVVMTDGTEYTTRSTYGKAGDTLNLDIDPKTH
                     PAWTGGSQTLLDRGGRLSRFNQKFGGLSFAKK"
     gene            6465..6995
                     /locus_tag="PK812_09190"
     CDS             6465..6995
                     /locus_tag="PK812_09190"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRJ69771.1"
                     /translation="MNLANWLTLLRLCLVPLVVLSIIQHWWQIAFLAFAVAGVSDAVD
                     GYVARRFNQVSELGALLDPIADKALLVSIYVTLAIAGAIPAWLTILVVSRDMMIVGAV
                     IVSWILGNPVEIRPIYVSKANTVAQIVFAGFMLFASAFTLNLQVLYWPLIAAVCVLTS
                     ASMVAYLVSWARHMTE"
     gene            6995..>7612
                     /locus_tag="PK812_09195"
     CDS             6995..>7612
                     /locus_tag="PK812_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69772.1"
                     /translation="MVRRPAPGAPAQLPLDLPLEPRFGREDFLVGPSNEQAYTLVDLW
                     PGWPERILILSGPQGSGKSHLAAIWAVQAKARVLSARSLATADPSALAEAGAIVVEDA
                     DASPVAETPFFHLINLVRERQAYLLVTARSAPDLWGIRTPDLISRLRLAPRLTIEPPD
                     DALLRALIVKQFLDRQIVIDTGVVEYLLTRMERSFAGAQRVVEALD"
BASE COUNT         1342 a         2521 c         2438 g         1311 t
ORIGIN      
        1 gcgctgcccg agttgctggt caagctgcgc cgccagcggc cgcgcgaagg catccgttcc
       61 gccgcccggc gggaaaggca caacgatggt gatcggcttg gatggccagg tctggcccag
      121 cgccggcagc gcggcgggca gcgtggcggc cgcaagcacg gcgcacagaa gcgagcgacg
      181 caacatgaaa tttctccctc ggttggtttt cttggcgaca agcgccgctt gccaagaaat
      241 ccaccgccgg aggaaccgac gcaaggagaa aatgcgagtg ccaagcttcg ggccttctca
      301 agcgccgccg ggtggctaat cttgggaccg aacagaaagg acctgtccat gaccacccgc
      361 cctgacgccg tactcgatgt gccaagaccc gcctggtgga gcgaggacat cgcgatgttg
      421 cgcgagatgg cggagaaatt ctgccagtcc gagatcgtgc cgcattatga ccgcttcgag
      481 acgcaggaga aagtggaccg cagcctttgg aacgcggccg gcgccagcgg ctttctctgt
      541 gcctcgatcc cggaggccta tggcggcgcc ggcggcagct acgggcacga ggcggccatc
      601 gtcgaggcgc tgggccatac cggcgcggac ggcttcggcc tgccgctgca caacgccatc
      661 gtagccccct acatccttca ctatggctcg gaggagcaga agcgccgctg gctgccccgc
      721 ctcgccagcg gcgagcttgt cggcgccata gccatgacgg aacccggcgc cggttcagac
      781 cttcagggcg tcaagaccac cgcccgcaag gatggcaatc actacgtcgt ctccggctcc
      841 aagacgttca tcaccaatgg ccagcacgcc aacctgatca tcgtcgtcac caagacggat
      901 gcatcggccg gggcgcgtgg cacctcgctc gtcgtggtgg aaaccgacga cgcgcccgga
      961 ttcgagcgcg gccgcaacct cgacaagatc ggcatcaaga cgaacgacac ctccgaattg
     1021 ttcttcaacg acgtgcgcat ccccaccgcc aatctgctcg gcccgcagga aggccaggga
     1081 ttcttccagc tcatgagtca gctgccgcag gagcgcctgc tcatcgccaa ccaggccatg
     1141 gcgatgatcg agcgcgcgct ggccgtgacc atcgcctatg tcaaggaacg caaggtcttc
     1201 ggcaagccgg tgctggagtt ccagaatacc cagttcaagc ttgcggaact gaagtccgag
     1261 gcgacgatga tgaaggtttt cgtcgaccac tgcgtcggcg aactgatcgc cggccggctg
     1321 accagcacga cggcttctat ggtgaagtac ctcgctaccg atctccagtg ccgcgtcgtg
     1381 gacgagtgcc tgcaactcca tggcggctac ggctacatga atgagtatcc gatcgcccgc
     1441 atgttccgcg acgcgcgcgt ccaacgcatc tacggcggca ccaacgagat catgaaggta
     1501 ctgatcgccc gcagcctgtg agcgccatga gcaacgcttt ccattccatc cgctacgagg
     1561 tggccgacag gatcgccacg atcacccttg atcggccgga acgcctcaac gccattgacc
     1621 ggcacatgcc atcggagatc gaggcggcgg ttgcgcacgc caatgccgac gatggcgtgc
     1681 acgtcatcgt gttacaaggg gcgggcaagg ccttctgcgc cggctatgac ctgaaggaat
     1741 tcgccgaaca gggcggcacc aatcccggcg tgcaggacat gccgtgggac ccgatgctcg
     1801 acttcaggtt catgtacggg aacacgcaga agttcatggc gctctggcgc tcctacaagc
     1861 cgaccatcgc caaggtgcac ggccatgcgg tggcgggcgg atcggacatc gccacctgct
     1921 gcgatttgat cgtcatggcg gacgacgcac ggattggata tccccctgcc cgcgtctggg
     1981 gctgcccttc ggtcggcatg tgggtctttc gcgtcggcgc gcagaatgcc aagcgactgc
     2041 tgttcaccgg cgatctcatc tcggcggcgg acgcgaaatc catggggctc gcgctggagc
     2101 accatcccgc cgccgaactt catgacgctg tggctcggct ggcgaaacgc atctctggcg
     2161 tgccgaagaa ccagttgatg atgcacaagc tgatgatcaa ctcggcgctg gagaacatgg
     2221 gcctgaccac gactcagcag atggccacgc tgttcgacgg catcacgcgg cattcgccgg
     2281 aaggcatgtg gtggaaggcg cgcgccgagg aggtcggctt ccagcaggcg gtgaaggagc
     2341 gcgactccgg cgatcccatc gcctcgcagg cggaaaagcg cctgccgggg ttcaggagct
     2401 gaggcgtccg aaacggctgc gccagccgtc caccgccgta tccagcgcgg cgaggcattc
     2461 cgcggtgtag ccggcatagt cgacccccgg cgcggcgaga tgcagttcgg agaccatgct
     2521 ccacatcgcc tcgcgcagaa gcgatgcgcc ctgcatggcg gcgaaggcgc gcttaagcgc
     2581 agcatccggc gcagcgccga aataatgcgc cagcagcgcc tcggcctcct cgctccccat
     2641 gccggcgttg gaggcaaggc ccgccagatc gaacagcggc gacgagaacc cggcgtattc
     2701 gaaatcgatc agccagagcc ggtgcccatc gtccaggatg ttctgcggca ccagatcgtt
     2761 atggccgaag atcagctccg aaggcagttg cgcggcgtgg aattcggccg ccagccgcga
     2821 cagcgcgggc agcctgtccc ggaaccggct gccgccggcg gcaagcgtgc gggcataatc
     2881 gttgatggtg tggaacaccc agaacaggtt cggccgaccg ctgacgcgct ccggcatgga
     2941 gcgatgaaag cggcgcacca gatcgatcac ctgctccgca ttcgcccgga tgtcgtgcgc
     3001 gccataggtg cacgccgcga tgaaccggct caccagcacg ccgggcgcat cgtagatcac
     3061 ttctgggcca agtccggacg catgggcggc gcgagcgacc atcgcctcat gatcgcggta
     3121 gacatgatgc accggataat cgtcgccgaa gcgaacgaca taccgcgcgc cgtcgctttc
     3181 gacccggtag ctctcgttgc tgcgcccacc cgtcagcggc tcgatgagcg gctccccgcg
     3241 ccagagcggc agggcgagaa tgcgcgcgac ggcggcttca ggaatgaccg gcgaaggcat
     3301 gtctgtcatc ggccgagatt aggtcgcccc ccgcggcgct cgcaagccga ttgtgcggcc
     3361 ggactgcgtc agaacccgta ccgcccgggg tcggccagca ccgcatcgac gatgcctgcc
     3421 gcatccgcga catcgacctt ttcgagcagg gcgatcatca cggcgcgatc ccggcgccgc
     3481 gccgccttgt cccactcccc cagcaagccg gcgaccgtca accgctcgtt gacggtcatt
     3541 ccgccgtatc gcgccgttgg cctgcctgtc atgccggtca gaacaccacc acggagcgaa
     3601 tagacttgcc cgcgtgcatc agatcgaagg cggtgttgat ctcattgagc ggcatggtgt
     3661 gggtgatcag gtcgtcgatg ttgatcttgc cctccatgta ccagtcgacg atcttcggga
     3721 cgtcggtgcg cccgcgcgcc ccgccgaacg ccgtcccctt ccagacgcgc ccggtgacca
     3781 gctggaacgg ccgcgtggcg atctccttgc cggactccgc gacgccgatg atgatgctct
     3841 cgccccagcc gcgatggcag cattccagcg cctgacgcat ggtcgtggtg ttgccgatgc
     3901 attcgaagga aaagtccgcg ccgccgccgg tgaggtccac cacggcctgg acgaccttgt
     3961 cgcggccgat ctcgtccgga ttgacgaaat gcgtcatgcc gaactttctc gccatttccg
     4021 ccttggccgg gttgagatcg acgccgatga tcttgtccgc gccgaccatg cgggcaccct
     4081 ggatgacgtt gaggccgatg ccgccgaggc cgaagaccgc gacattggcg ccaggccaga
     4141 ccttcgccgt atggatcacc gcgccgacgc cggtggtgac gccgcagccg atgtagcaga
     4201 tcttgtcgaa tggcgcgtcc tcgcggacct tcgccactgc gatttccggc agcacggtga
     4261 aattcgaaaa agtcgagcag cccatgtagt ggaacacttc gccgccgtcg caggagaagc
     4321 gcgacgtacc gtccggcatc acgccctgcc cctgggtggc gcggatcgcc gtgcacaggt
     4381 tggagcgctg cgacaggcag gttttgcact gccggcattc cggcgtgtag agcggaatga
     4441 cgtgatcgcc cggcttcacc gaggtgacgc cggcgccgac ctcgcgcacg attcccgctc
     4501 cctcatgccc gaggatagcc gggaactttc cctcggaatc gagaccggac agcgtgtagg
     4561 catcggtatg gcagacgccg gtcgccatga tctcgaccag cacctcgccg ggcttcggcc
     4621 ccgcaatgct gattgtctcg atggtcagtg gcgtgttcgc tgcccatgcg acggcagccc
     4681 tcgacgtgat catgcgttct ctcccaaggt gcgccgacag gctagccggt tcgttgcgcc
     4741 gtcttcaata cggcaggccg acataattct ctgcgagcga gcgctgcgcc gcttccgacg
     4801 agatgagata ggcgaaatcc gtgcgctgca tgcggcggtc gaatccggaa acctgcggga
     4861 atgtgtgcag caggttggtc atccaccacg agaagcgctc ggccttccag atgcgcgcca
     4921 gcgcccgctg cgaataggca tcgagcccgg actggctgct cgaccgatag tagtcgacaa
     4981 agccgtcgaa cagatagcgc acatcgctgg cggcgaggtt gaggcccttc gcgccggtcg
     5041 gcggcacgat gtggccggca tcgcccgcca ggaacagcgt accgaaacgc agtggctccg
     5101 ccacgaaact cctcaatggc gcgatcgact tctcgatcga cggaccggtt tgcaccgtgg
     5161 cggcgatctc gggaccaagt cggcgcttga gctcggccca gaacagatcg tccgaccagg
     5221 catcgaccgt gtcgtccagg ctgcactgga gatagtagcg gctgcgggtc agcgagcgct
     5281 gcgaacacag agcaaacccg cgctcgttat gcacgtagat cagctcatga tggcacggcg
     5341 gcacgtcggc cagaaggccg agccagccaa acggatagac acgttcgaac cgggtgatcg
     5401 ccccggccgg caccgaggcc cggcagatgc cgtggtagcc gtcacagccc gcgatgaaat
     5461 cgcacttgat gtcgtacggg cggccttcgt gccagaacgt cacgcacggg ctggaccggt
     5521 cgatatcgtg gatggcgacg ttttcggccg cgtagaacgt cggagtgccg tcagcctgcc
     5581 gcgcctccat gaggtcccgg gtgatttccg tctggccata gaccatgacc gtcttgccgc
     5641 cggaatgggc ggcgaggtcg atccgatgcc gctcgccgtc aaaaacgatg tcgaaccctt
     5701 cgtggaccag gccctcgcga tgcatgcgcg ccgcgacgcc ggcctcctcc agcatggctg
     5761 cggcgccctg ctccagcacg ccggcgcgga tgcgtccgag gatgtattcc ttgggcttgt
     5821 tgtcgacgat gacattcgag atgccggcgc gcgacagaag ctgcccaagc aacaggccgg
     5881 atggacctga tccgatgatg gcaacctgcg tgcgcatggg cgtctccctg aaggtgtttt
     5941 caggacccta agtgcccttc ggcccggaga caatcggcca gctgtcgcag gcgccggcat
     6001 gcacgccaaa aaacaaaggc cccgtctccg gggccttgga acacgcgaca ggatgctgcg
     6061 cggcttactt cttggcgaag ctgaggccgc cgaacttctg attgaagcgc gacaggcgtc
     6121 caccgcggtc gagcagggtc tgcgacccgc cggtccaggc cggatgggtc ttggggtcga
     6181 tatcgaggtt cagcgtgtcg ccagccttgc cgtaggtcga ccgcgtcgtg tactcggtac
     6241 cgtcggtcat cacgaccttg atgaaatggt agtcaggatg cgtttcggct ttcatggcgg
     6301 tctctccggg agcaggccta atgcgacgct gaacgcccgg ctgctgcata tgctgaattg
     6361 aagatgcgcc gcattatacg aatcccctcg cgagggcaag agcgcctatc gcccggcttg
     6421 ctctcgccat cgcgctgcgc cggcgctaga actacgccat gggaatgaat ctcgccaact
     6481 ggctgactct gttgcgcttg tgcctggtgc ccctggtggt cctgtcgatc atccagcatt
     6541 ggtggcagat cgctttcctg gcgtttgccg tggcgggcgt atcggacgcg gtcgacggct
     6601 atgtcgcgcg gcgcttcaac caggtgtccg aactcggtgc cctgctcgat ccgattgccg
     6661 acaaggcact gctggtctcg atctacgtga cgctggccat cgccggcgcc attcccgcct
     6721 ggctgacgat cctcgtcgtg tcgcgtgata tgatgatcgt gggcgcagtc attgtttcct
     6781 ggatcctcgg caacccggtg gagatccgcc cgatctacgt ctcgaaggcc aatacggtgg
     6841 cgcagattgt ctttgccggc ttcatgctgt tcgcctcggc cttcacgctg aacctccagg
     6901 tcctgtattg gccgctgatt gctgcggtct gcgttctgac aagcgcgtcc atggtcgcct
     6961 atctggtgag ctgggcgcgc cacatgaccg agtgatggtg cgccggcccg ctccgggagc
     7021 gccggcccag ttgccgctgg acctgccgct ggaaccgcgc ttcgggcgcg aggatttcct
     7081 tgtcggcccg tccaacgagc aggcctacac gctggtggac ctgtggcccg gctggcctga
     7141 acggatactg atcctgagcg gcccgcaggg ctcgggcaag tcgcacctcg ccgcgatctg
     7201 ggccgtgcag gcgaaggccc gcgtgctttc ggcccgctct ctcgccaccg ccgacccttc
     7261 agcactggcc gaggccggcg ccatcgtggt ggaggacgcc gacgccagcc ctgtcgccga
     7321 gacgccgttc tttcacctga tcaacctcgt ccgggagcgc caggcctatc tgctcgtcac
     7381 ggcgcgttcc gcgcccgacc tgtggggcat ccgcacgcct gacctgattt cgcgtctgcg
     7441 cctcgcgccg cgtctgacca tagaaccgcc ggacgacgcg ctgctgcgtg ccctcatcgt
     7501 caagcagttc ctcgaccggc agatcgtcat cgacacgggc gtggtcgaat atctgctcac
     7561 ccggatggag cgctccttcg ccggagcgca gcgcgtggtc gaggcgcttg ac
//
LOCUS       DAOMXN010000123         7587 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_5054_length_7587_cov_11.659586, whole genome shotgun sequence.
ACCESSION   DAOMXN010000123 DAOMXN010000000
VERSION     DAOMXN010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 7587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7587
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5054_length_7587_cov_11.659586"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(223..996)
                     /locus_tag="PK812_09200"
     CDS             complement(223..996)
                     /locus_tag="PK812_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009209440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HRJ69773.1"
                     /translation="MSAVANLKPSPLNNEVAVEMRGVHKWYGEFHVLRDINLKVTRGE
                     RIVVCGPSGSGKSTMIRCINRLEEHQQGDIVVDGIELTNDLKKIDEIRRDVGMVFQHF
                     NLFPHLTILENCTLAPIWVKKMPKKDAEEVAMHYLKRVKIPEQANKYPGQLSGGQQQR
                     VAIARSLCMSPKIMLFDEPTSALDPEMVKEVLETMVSLAQEGMTMMVVTHEMGFARQV
                     ADRVIFMDAGQIVEMNKPDLFFKNPQHERTKLFLSQILH"
     gene            complement(1012..2370)
                     /locus_tag="PK812_09205"
     CDS             complement(1012..2370)
                     /locus_tag="PK812_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009492609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRJ69774.1"
                     /translation="MSAKAMTSPAYVQQSALPALPPPASTVGVIGWMRNNLFSSPTNI
                     ALTIFGLFLAYVTIPPIIKFLVLDAVWTGTSREACLYKDGVEVGACWAFVRDRLAYTL
                     YGSYPVDGRWRCDAFMVLGAIAIFWVLHPRAPHKSWGMLFAFAFTPLASYGLLSGNNP
                     LAFLLEPLAIFKGGDTLVISVLVTLAIFAAGVLLARMEGLEMPKAIGAGVLAVVGAAL
                     VGFIFAAIWATFKLQPVETARWGGLTVTLVVAAVGIVVSLPLGILLALGRRSKLPIVK
                     FFSVMFIEFVRGVPLITMLFMANTMLPLFLPERYAPDKLLRALVGIALFASAYMAEVV
                     RSGLQALPKGQYEGAMAMGLSYGQMMRLIILPQALKVAIPNIVNTYIGLLKDTSLLLI
                     VGIFDFLKAIENARIDPQWAAPTTSATGYAYAAIIYFIMCYGMARYAKNMEKRLAVAD
                     KR"
     gene            complement(2381..3565)
                     /locus_tag="PK812_09210"
     CDS             complement(2381..3565)
                     /locus_tag="PK812_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRJ69775.1"
                     /translation="MSSAASGGRPKVSPINDPKIRGYVFQAVLFALVVFLLYEAYDNA
                     IENLRKAKIASGLGFWNNTAGFDIGQRLIAYTATSTYGRAFWVGLLNTLLVASIGIVL
                     ATILGFLIGIGRLSKNWIVNKLSTIYVEVIRNTPLLLQLLFWYNAVLKALPEVRDSIA
                     LPGGAYLNNRGIFTPEPVFGAAFSNVLWAFLAGLAGTIAFRVWARRRQESTGQQAPVF
                     LVSLALIIGLPLLVYFLSGSPLTFNEVQKGRFNISGGVQIFPEFGALLLGLVIYTAAF
                     IAEAVRAGIQAVNRGQTEASYALGLRPGPTLRLVIIPQAMRVIIPPLTSQYLNLTKNS
                     SLAVAIGYPDLVQVFTGTVLNQTGQAVEVVAITMLVYLTISLTTSLIMNWYNARKALV
                     ER"
     gene            complement(3686..4699)
                     /locus_tag="PK812_09215"
     CDS             complement(3686..4699)
                     /locus_tag="PK812_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017964125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HRJ69776.1"
                     /translation="MKKTIISATFGAILAFAGAAQAQTLNDVKARGVVNCGANTGLAG
                     FGTPDAQGNWTGFDVDYCRAIAAAVFGDGNKVKFVPLSAKDRFTALQSKEVDVLVRNT
                     TWTSSRDTSLGLNFAGVNYYDGQGFMVRKKLKVQSALELSGASVCTQQGTTTELNLAD
                     FFRARNMKFEAVVFATNDETIKAYDAGRCDAFTTDASGLYAERLRLANADDHIVLPEI
                     ISKEPLGPATRHGDDQWYDIVKWTHNVLLTAEELGVTKANVDQMKTSTNPDVKRLLGV
                     EGEFGKALGLSNDFAVSVIKAVGNYGELFERNVGAGSRLKIARGQNALWNKGGLQYAP
                     PIR"
     gene            complement(4960..5976)
                     /locus_tag="PK812_09220"
     CDS             complement(4960..5976)
                     /locus_tag="PK812_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HRJ69777.1"
                     /translation="MKKLVLSAVLGAAAVAGASVADAQVLNTVKQRGVVQCGVGTGLA
                     GFGIPDAQGNWAGLDVDYCRAIASAVFNDPSKVKFVPLSAKDRFTALQSGEVDVLIRN
                     TTWTMSRDTSLGLNFSAVNYYDGQGFMVRKKLNIKSAKELAGASVCTQQGTTTELNLA
                     DYFRANNLKYEAVVFATNDETIKAYDAGRCDAFTTDASGLYAERLRLANPDDHMVLPD
                     IISKEPLGSSTRHGDDQWFDLVKWVHYALITAEELGVTKANVDQMKGSANPEIKRLLG
                     VEGEFGKALGLDNAWAFNAIKHIGNYGEMFERNVGMASRLKIARGQNALWSKGGLQYA
                     PPIR"
     gene            complement(6160..6498)
                     /locus_tag="PK812_09225"
     CDS             complement(6160..6498)
                     /locus_tag="PK812_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HRJ69778.1"
                     /translation="MLIKLSQDVGGYLYEVFIDKADLWLLIGVGGQLLFAARFIVQWI
                     ASERAGRSVVPLAFWLFSIGGGMITLVYGIHKKEPVIILGQALSIFIYVRNLVLIARE
                     KRRGRQSPPA"
     gene            complement(6488..7246)
                     /locus_tag="PK812_09230"
     CDS             complement(6488..7246)
                     /locus_tag="PK812_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017483948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRJ69779.1"
                     /translation="MTTSHDGARPVLSVVVPVKNEQDNIAPLVTEIATAAFSLGVFEV
                     IYVNDGSGDRSAAVLDELAASRPWLRHIRHESSAGQSAAVRTGVRHARADIVCTLDGD
                     GQNDPAFIPRLVQALREGGAGVGLVQGQRVGRKASGFKRLQSRVANGVRSRILKDATR
                     DTGCGLKCFWREAYLALPYFDALHRFMPALMTREGYRVAHVDVVDRPRLTGVSNYGFW
                     NRLWVGILDLAGVWWLIRRKRTTPVAQEAPRHAH"
     gene            complement(7299..>7587)
                     /locus_tag="PK812_09235"
     CDS             complement(7299..>7587)
                     /locus_tag="PK812_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HRJ69780.1"
                     /translation="AVGCDKPALASAGYREPSLVFLTRTDLRLVMGAEAANILAQDGP
                     GCRIAFVESREEASFRAALAAKPAGETLTTRIRGVNINGGRTLDIGVYVRR"
BASE COUNT         1330 a         2408 c         2536 g         1313 t
ORIGIN      
        1 gcgggacgat tgcccggtgg gaacggcgaa ggcctgacgg cgcctcggcg cgctgcggcc
       61 gcatgccccc gaaccgggcg cggcccagac ttgcgcggtc tgccccctca taccaagggt
      121 catgacaatg gacccttggg tcctgtctcg aactttcctt gcgcattgta aaacaaggaa
      181 aattcaagag cagccatcgg tcatccctga tgaccgatgg tatcagtgca ggatctggct
      241 gaggaacagc ttcgtgcgct cgtgctgcgg gttcttgaag aacaggtccg gcttgttcat
      301 ctcgacgatc tggccggcgt ccatgaagat cacacggtcg gcgacctgac gggcgaagcc
      361 catttcgtgc gtcaccacca tcatggtcat gccttcctgc gccagcgaca ccatggtctc
      421 cagcacttcc ttgaccattt ccgggtcgag cgccgaggtc ggctcgtcga acagcatgat
      481 cttcgggctc atgcagagcg agcgggcgat ggccacgcgc tgctgctggc cgccggagag
      541 ctgtccggga tacttgttgg cctgctccgg gatcttgacg cgcttgaggt agtgcatcgc
      601 cacctcttcg gcgtccttct tcggcatctt cttcacccag atcggggcga gcgtgcagtt
      661 ttccaggatg gtgaggtgcg ggaacaggtt gaagtgctgg aacaccatgc cgacgtcgcg
      721 gcggatctcg tcgatcttct tgaggtcgtt ggtgagttcg atgccgtcga cgacaatgtc
      781 gccctgctgg tgttcctcca ggcggttgat gcagcggatc atggtcgact tgccggaacc
      841 ggacgggccg cagacgacga tgcgctcgcc gcgcgtcacc ttcaggttga tgtcgcgcag
      901 cacgtggaat tcgccgtacc acttgtgcac gccgcgcatt tccacggcga cctcgttgtt
      961 cagaggcgac ggcttgagat tggcgactgc tgacatgttc tcccccaagt cttaccgttt
     1021 gtccgccacc gccagccgct tttccatatt cttggcgtag cgggccatgc cgtagcacat
     1081 gatgaaatag atgatcgccg cataggcata gccggtggcc gatgtggtcg gcgcggccca
     1141 ttgcgggtcg atgcgggcgt tctcgatggc tttgaggaag tcgaagatgc cgacgatcag
     1201 cagcagcgac gtgtccttca acaggccgat ataggtgttg acgatgttcg ggatcgccac
     1261 cttcagcgcc tgcggcagga tgatcaggcg catcatctgc ccgtagctga ggcccatggc
     1321 catggcgccc tcgtactggc ccttcggcag ggcctgcagg ccggagcgca ccacttcggc
     1381 catgtaggca gaggcgaaca gcgcgatgcc caccagcgcg cgcagcagct tgtccggcgc
     1441 atagcgctcg ggcaggaaca gcggcagcat ggtgttggcc atgaacagca tggtgatcag
     1501 cggcacgccg cgcacgaact cgatgaacat caccgagaag aacttcacga tcggcagctt
     1561 cgagcggcgg ccgagcgcca gcaggatgcc gagcggcagc gagacgacga tgccgaccgc
     1621 cgccaccacc agagtcacgg tcaggccgcc ccagcgcgcg gtctcgaccg gctgcaactt
     1681 gaaggtcgcc cagatcgcgg cgaagatgaa gccgaccagc gccgcgccga cgaccgccag
     1741 cacgccggct ccgatcgcct tcggcatttc cagcccctcc atccgcgcca gcagcacccc
     1801 ggcggcgaag atggccagcg tcaccagcac ggaaatcacc agggtgtcgc cacccttgaa
     1861 gatcgcgagc ggctccagca ggaaggcgag cgggttgttg ccggagagca ggccgtagct
     1921 cgccagcggg gtgaaggcga aggcgaagag catgccccag ctcttgtgcg gggcgcgcgg
     1981 atgcagcacc cagaagatgg cgatggcgcc gagcaccatg aaggcgtcgc agcgccagcg
     2041 cccgtccacg gggtaggagc cgtagagcgt gtaggcgagg cggtcccgga cgaaggccca
     2101 gcaggcgcca acctccactc cgtccttgta gaggcaggcc tcgcggctcg tgccggtcca
     2161 aaccgcgtcg agcaccagga acttgatgat cggcggaatc gtcacatagg cgaggaaaag
     2221 cccgaaaatg gtcagcgcga tgttggtcgg gctggagaac aggttgttcc gcatccagcc
     2281 gatcacgccg acggtcgagg ccggcggggg cagcgcgggc agcgcgctct gctgcacgta
     2341 ggcgggcgag gtcatggcct tggcgctcat tgttgcctcc tcaccgttcc accagcgcct
     2401 tgcgggcgtt gtaccagttc atgatgagcg aggtcgtcag gctgatcgtg aggtagacca
     2461 gcatggtgat cgccaccact tccaccgcct ggccggtctg gttcagcacc gtgccggtga
     2521 acacctgcac gagatcggga tagccgatgg ccaccgccag cgaggaattc ttggtcaggt
     2581 tgagatactg gctggtcagc ggcgggatga tcacgcgcat ggcctgcggg atgatgacaa
     2641 gacgcagcgt cggaccggga cgcagcccga gcgcatagga ggcttcggtc tgcccccggt
     2701 tgacggcctg gatgccggca cgcaccgcct cggcgatgaa ggcggcggtg tagatcacca
     2761 gaccgagcag cagcgcgccg aattccggga agatctgcac gccgcccgag atgttgaagc
     2821 ggcccttttg cacctcgttg aacgtgagcg gcgatcccga caggaagtag accagcagcg
     2881 gcagcccgat gatgagcgcg agggagacga ggaacacagg cgcctgctgg cccgtggact
     2941 cctggcgccg gcgtgcccag acgcggaagg caatggtgcc cgccaggccc gccaggaagg
     3001 cccataacac attggaaaag gccgcgccga acaccggctc cggcgtgaag atgccgcggt
     3061 tgttgaggta ggccccgccc ggcagcgcga tgctgtcgcg cacctccggc agcgccttca
     3121 gcaccgcgtt gtaccagaac agcagttgca gcagcagcgg cgtgttgcgg atgacctcga
     3181 cgtagatggt cgacagcttg ttgacgatcc agttctttga cagccggccg atgccgatga
     3241 ggaagccaag gatcgtcgcc agcacgatgc cgatcgacgc caccagcagg gtgttcagca
     3301 ggcccaccca gaaggcgcgg ccgtaggtcg aggtggcggt gtaggcgatc aggcgctggc
     3361 cgatgtcgaa gccggcggtg ttgttccaga agccgagccc cgaggcgatc ttggccttgc
     3421 gcaaattctc tatggcgttg tcgtaggcct cgtagaggag gaagacgacc agcgcgaaca
     3481 ggacggcctg gaaaacgtag ccgcgaatct tcgggtcatt gatgggtgag accttgggcc
     3541 gcccgccact ggctgctgaa gacacgtaca cctcccctac ccagacgccg gcccccgacg
     3601 cgccggcgcc caggaccaat gaccctttcc gggtcttttc ttatgcaaga aacgggccgg
     3661 cggcgcgccg ccggcccgga actcatcagc ggatcggcgg agcgtactgc agaccgccct
     3721 tgttccacag cgcgttctgg ccgcgcgcga tcttcaggcg cgagccggcg ccgacattgc
     3781 gctcgaacag ttcgccgtag ttgcccaccg ccttgatcac gctgacggcg aagtcgttgg
     3841 acaggccaag ggccttgccg aactcgccct cgacgcccag caggcgcttc acgtccggat
     3901 tggtggaggt cttcatctgg tcgacgttgg ccttggtcac gcccagttcc tcggccgtca
     3961 gcagcacgtt gtgcgtccac ttgacgatgt cgtaccactg gtcgtcgcca tggcgggtcg
     4021 ccggtccgag cggctccttg gagatgatct ccggcagaac gatgtggtca tccgcattgg
     4081 ccaggcgcag gcgctcggcg tagaggccgg aagcgtccgt ggtgaaggcg tcgcagcggc
     4141 cggcgtcata ggccttgatg gtctcgtcgt tggtggcgaa gaccacggcc tcgaacttca
     4201 tgttgcgggc gcggaagaag tcggcaaggt tcagttcggt cgtggtgccc tgctgggtgc
     4261 agaccgaggc gccggagagt tcaagcgcgg actgcacctt gagcttcttg cgcaccatga
     4321 agccctggcc gtcgtagtag ttcacgccgg cgaagttcag gccgagcgag gtatcgcgcg
     4381 acgaggtcca ggtggtgttg cggacgagca cgtccacttc cttggattgc agcgccgtga
     4441 aacggtcctt cgcggacagc ggcacgaact tgaccttgtt gccgtcgccg aacaccgccg
     4501 ccgcgatggc gcggcagtaa tcgacgtcga agccggtcca gttgccctgg gcatccggcg
     4561 tgccgaagcc ggcgaggccg gtgttcgcgc cgcagttgac cacgccgcgc gccttgacgt
     4621 cgttcaacgt ctgcgcctgc gccgcgccgg caaaggcgag gatggcgccg aacgttgcgg
     4681 aaataatggt tttcttcatg acacgtgttc tcccctgtgt cggtgatgcg aaagttttgg
     4741 atggatcgcc ggggcacatg ggcctgatcg cggcagccct gcccttgcgg atcctcaggg
     4801 tcgcccctga ctggtgctta tctcatgtgc ttttcgcggc agggtcaagc cactccggcc
     4861 gatccgcgtc gaaaaagcaa aacccagcaa caatttgcca gaatatggga aaatcaggct
     4921 gccgcctccg cacatggcgg cggcagcctg cacaatcgat cagcggatcg gcggagcgta
     4981 ttgcaggccg cccttgctcc acagcgcgtt ctggccgcgg gcgatcttca ggcgggaagc
     5041 catgccgacg ttgcgttcga acatctcgcc gtagttgccg atgtgcttga tggcgttgaa
     5101 ggcccaggcg ttgtcgaggc cgagcgcctt gccgaattca ccctcgacgc cgagcagacg
     5161 cttgatctcc ggattggcgg agcccttcat ctggtccaca ttggccttgg tgacgccgag
     5221 ttcctccgcc gtgatcaggg cgtagtgcac ccacttcacg aggtcgaacc actggtcatc
     5281 gccgtggcgg gtggacgagc cgagcggctc cttcgagatg atgtccggca gcaccatgtg
     5341 gtcgtccggg ttggccaggc gcaggcgctc ggcgtagagg cccgaggcgt ccgtggtgaa
     5401 ggcgtcgcag cggccggcgt cataggcctt gatggtctcg tcgttggtgg cgaagacgac
     5461 cgcctcgtac ttcaggttgt tggcccggaa gtagtccgcc aggttcagct ccgtcgtcgt
     5521 gccctgctgg gtgcagacgg acgcgccggc cagttccttg gccgacttga tgttgagctt
     5581 cttgcgcacc atgaagccct ggccgtcgta atagttcacg gccgagaagt tgaggccgag
     5641 agaggtgtcg cgcgacatcg tccaggtcgt gttgcggatc agcacgtcga cctcgccgga
     5701 ctggagcgcg gtgaagcggt ccttggccga caggggcacg aacttcacct tggaggggtc
     5761 gttgaagacc gccgaggcga tggcgcggca atagtcgacg tcgaggccgg cccagttgcc
     5821 ctgcgcgtcc ggaatgccga agccggccag gccggtgccg acgccgcatt gcacgacgcc
     5881 gcgctgcttc acggtgttca acacctgggc gtccgcgacg ctggcgcccg ccacggcggc
     5941 ggcgcccaga acagccgaaa gaaccagttt cttcatgagc gattgctccc ttgttttcgt
     6001 cctgagccga aaaggtcgtc tcacacgcct tccggcaacg cgcccgtgtc cgccctcctc
     6061 ggcgtcaacc cggacttgcg ccctcacttg gccagatttc gctgctggtc aagactgcga
     6121 tggcccaaac atcaaacttt tgtcgtatgc agcgccgatt caggccggcg gggactggcg
     6181 gccgcgccgc ttttcccggg cgatcagcac caggttgcgc acatagatga agatcgacag
     6241 ggcctgcccg aggatgatca ccggctcctt cttgtggatg ccgtagacca gcgtgatcat
     6301 gccgccgccg atggaaaaca accagaaggc gagcggcacg acgctgcgcc cggcccgttc
     6361 gcttgcgatc cactgcacga tgaagcgcgc cgcgaacaga agctggccgc cgacgccgat
     6421 cagcagccag aggtcggcct tgtcgatgaa cacttcgtag aggtagccgc cgacgtcctg
     6481 cgaaagctta atgagcatgg cgcggggcct cctgcgccac aggggtggtg cgcttgcgcc
     6541 ggatcagcca ccagaccccg gcaagatcga ggatgccgac ccagagccgg ttccagaacc
     6601 cgtagttcga cacgccggtc aggcgcggcc ggtcgacgac atcgacatgg gcgacgcgat
     6661 agccttcccg cgtcatcagc gcgggcatga agcggtgcag cgcatcgaaa tagggcagcg
     6721 cgagataggc ctcgcgccag aaacacttca ggccgcagcc ggtgtcgcgc gtggcgtcct
     6781 tcaggatgcg actgcgcacg ccgttggcga cgcgcgactg caagcgcttg aagccggacg
     6841 ccttgcgccc gacccgctgg ccctgcacca gcccgacgcc ggctcccccc tcgcgcaggg
     6901 cctgaaccag gcgggggatg aaggccggat cgttctggcc gtcgccgtcc agcgtgcaca
     6961 cgatgtcggc gcgggcgtgc ctgacgccgg tacgcacggc ggcggactga ccggcgctgg
     7021 actcgtggcg gatatggcgc agccaggggc ggctggccgc cagctcgtcg agaacggccg
     7081 cgctgcgatc gcccgaaccg tcattgacgt agatgacctc gaagacgccg agcgaaaacg
     7141 ccgcggtggc gatttcggtc accagcgggg cgatgttgtc ctgttcgttc ttcaccggca
     7201 caaccacgga aaggaccggg cgcgcgccgt cgtggctcgt tgtcatacgt tggatgtcca
     7261 ttggtcggac gggttcttat acgatgtccg ccggcctgtc agcgccgcac atagacgccg
     7321 atgtcgagcg tgcgtccgcc attgatgttc acgcccctga tgcgtgtggt caatgtctcg
     7381 cctgccggtt tcgcggcgag cgcggcgcgg aaagacgcct cctcgcgcga ttccacgaag
     7441 gcgatgcggc aaccgggccc gtcctgcgcg agaatgttgg cggcttccgc gcccatcacc
     7501 aggcgcaggt ccgtgcgggt caggaagacg aggctcggct cgcgatagcc ggcggaggcc
     7561 agcgccggtt tgtcgcagcc cacggcc
//
LOCUS       DAOMXN010000124         7481 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_5148_length_7481_cov_11.076084, whole genome shotgun sequence.
ACCESSION   DAOMXN010000124 DAOMXN010000000
VERSION     DAOMXN010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 7481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7481
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5148_length_7481_cov_11.076084"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1085
                     /locus_tag="PK812_09240"
     CDS             <1..1085
                     /locus_tag="PK812_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRJ69781.1"
                     /translation="ERARGDRKAWMDRLSASKQAGAFRAAISGDLPRDLDGAMATFKA
                     GLIADKPNVATRKASEMALGVINATIANTVGGSADLTHSNLTITKGMESVSAGAYGGR
                     YIRYGIREHIMGAAMNGLALHGGFIPYGGTFLVFSDYARGAIRLSALMEQRVVYVLTH
                     DSIGLGEDGPTHQPIEHLAMLRATPNLTVFRPCDAVETAECWELALAARRTPSVLALS
                     RQNLPVLRTEAGANRSERGAYVLRACGDKRDITLLASGSEVEIACAAADTLTAGGLQV
                     AVVSMPSWELFEAQDEAYRRAVLGTAPRIGIEAACRLGWDRWIGERGIFIGMTGFGAS
                     APAADLYRHFGITPAAIVDAADKLLG"
     gene            1106..2191
                     /gene="fba"
                     /locus_tag="PK812_09245"
     CDS             1106..2191
                     /gene="fba"
                     /locus_tag="PK812_09245"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031242.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HRJ69782.1"
                     /translation="MARITLRQLLDHAAERGYGVPAFNINNMEQVLAIMEAAKAVDAP
                     VILQASRGARSYANDIMLAAMVDAAERMFPDIPICLHLDHGNEEATCATAIQHGFTSV
                     MMDGSLEADGKTPADYAYNLAITRRVTDMAHWVGASVEGELGVLGSLEHGTGEQEDGH
                     GAEGKLSHDQLLTDPDQAVDFVSRTKVDALAIAMGTSHGAYKFSRKPDGAILAMHVIR
                     AIHEKLPNIHLVMHGSSSVPQDLQAEFNAHGGAMKATWGVPVEEIQTGIKYGVRKVNI
                     DTDCRLAMTAQFRKIAQENKAEFDPRKFLKPAMDAMRLLCRQRYEQFGSAGNASKIKV
                     VPLAEMAKRYRSGSLDPQVAGMSQAAA"
     gene            2244..3716
                     /locus_tag="PK812_09250"
     CDS             2244..3716
                     /locus_tag="PK812_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="form I ribulose bisphosphate carboxylase large
                     subunit"
                     /protein_id="HRJ69783.1"
                     /translation="MNVSLDTSVKELKGAERYKTGVIEYKRMGYWDADYVPKPTDVLA
                     LFRITPQDGVDPVEAAAAVAGESSTATWTVCWTDRLTAKEKYRAKAFRCEPVPGSPGQ
                     YFATIAYDLDLFEPGSIANLTASIIGNVFGFKPLKALRLEDMHFPVAYVKTFKGPATG
                     IVVERERLDKFGRPLLGSTVKPKLGLSGRNYGRVVYEGLKGGLDFMKDDENINSQPFM
                     HWRDRFLYCAEAVNKASAATGEVKGTYMNVTAGTMEEMYERAEFAKEIGSCIIMIDLV
                     IGYTAIQSMSNWSRKNDMILHLHRAGHGTYTRQKTHGVSFRVIAKWMRLAGVDHIHAG
                     TVVGKLEGDPNTTKGFYDILRQDYVHKNLSHGVFFDQPWASLNQCMPVASGGIHAGQM
                     HQLLDLLGEDTVLQFGGGTIGHPQGIQAGATANRVALEAMVLARNEGRDIVNEGPQIL
                     MDAAKWCAPLKQALDTWKDITFNYESTDAPDMVTTATPGR"
     gene            3775..4215
                     /locus_tag="PK812_09255"
     CDS             3775..4215
                     /locus_tag="PK812_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose bisphosphate carboxylase small subunit"
                     /protein_id="HRJ69784.1"
                     /translation="MAMTNTGKRITQGQFSFLPDLTDKQITAQIKYGLKNGWAWSVEY
                     TDDPHPRNTYWEMFGMPMFDLKDAAGVLSEVNSCRKTFPNHYVRLTAFDSSHGWESPR
                     MSFIVNRPKDEPGFLVERQERNGRAQGYAIRSYATNKPESERYS"
     gene            4508..5416
                     /gene="cbbX"
                     /locus_tag="PK812_09260"
     CDS             4508..5416
                     /gene="cbbX"
                     /locus_tag="PK812_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbX protein"
                     /protein_id="HRJ69785.1"
                     /translation="MSADPATQIDLKAEFAQTQIGEVLDALDRELIGLKPVKLRIRQI
                     AALLLVDRVRRKLGMTSGAPSLHMSFTGNPGTGKTTVALRMAEILHKLGYVREGHLIA
                     VTRDDLVGQYIGHTAPKTKEVLKRAMGGVLFIDEAYYLYRPENERDYGQEAIEILLQV
                     MENQREDLVVILAGYAERMETFFRSNPGFRSRVAHHIDFPDYESAELLDIADTMLAAQ
                     NYRFSKPGRAAFADYIAARREQPHFANARSIRNALDRARLRQASRHFESGKAVNLDDL
                     MTIDAADILASRVLTQNGALTPAARE"
     gene            5428..6093
                     /gene="rpe"
                     /locus_tag="PK812_09265"
     CDS             5428..6093
                     /gene="rpe"
                     /locus_tag="PK812_09265"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRJ69786.1"
                     /translation="MPRPLKIAPSILSADFGDLRADVAAMMAAGADWVHIDVMDGHFV
                     PNISFGAPIIKALRPATPALFDTHLMIAPVDAYLKDFADAGADLISVHVEAGPHIHRS
                     LQAIRALGKKSGVVVCPGTPASAVRPLLDVVDLVLCMSVNPGFGGQSFIPSVLETIRD
                     MRALIADRPIDLEVDGGVTPENARVVVEAGANVLVAGSAAFRGGRSAYAANVAAIRKA
                     ATA"
     gene            6090..6809
                     /locus_tag="PK812_09270"
     CDS             6090..6809
                     /locus_tag="PK812_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRJ69787.1"
                     /translation="MTWSARRGSTLPIEALIFDVDGTLAETEEFHRRAFNAAFTTLGL
                     DWFWDRPLYADLLKVTGGRERIRHFMDHYLSGTGEARDMVALLHQRKNRVYAGFVANR
                     QVPLRPGMRELIAAAQGAGLKLAIATTTSPANVDALLTAALGSGWREVFPVMVAGDMV
                     KAKKPAPDVYHAALARLRVAPEAALAIEDSRNGVLSATGAGLRVAAVRSAYTAQDDLS
                     GAVRVFDDPVDLTLASLRALA"
     gene            complement(6951..>7481)
                     /locus_tag="PK812_09275"
     CDS             complement(6951..>7481)
                     /locus_tag="PK812_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRJ69788.1"
                     /translation="RQFLAEAMIISLGGGAIGFACALVARHFGAQDLGVGETNGLRLR
                     TLRETGGFSAYAPGTPDEPAENSVDLVIDAVGAAATRAAASRLVRPGGVIVHLGLLPG
                     LDGLDVRKVTLQEVTFLGSYCYTARDFGEVVQALAARQLGSLAWFAQRDLADGARAFH
                     DIDANQIAAAKVVLRT"
BASE COUNT         1415 a         2489 c         2296 g         1281 t
ORIGIN      
        1 gcgaacgcgc gcgcggcgac cgcaaggcct ggatggaccg cctctccgcc tcgaaacagg
       61 cgggcgcttt ccgcgccgcg atctctggcg acctgccgcg cgacctcgac ggcgccatgg
      121 cgaccttcaa ggcaggcctg atcgccgaca agccgaatgt cgcgacgcgc aaggcctcgg
      181 agatggcgct tggagtcatc aatgccacga tcgcgaacac ggttggcgga tcggccgacc
      241 tgacgcattc gaacctcacc atcaccaagg gcatggagag cgtttccgcc ggagcttacg
      301 gcggccgcta catccgttat ggcatccgcg agcacatcat gggcgcagcg atgaatggcc
      361 tcgccctgca tggcggcttc atcccctatg gcggcacgtt cctggtgttc tcggactatg
      421 cgcgcggcgc gatccggctg tccgccctga tggaacagcg tgtcgtctat gtgctgaccc
      481 atgattccat tggccttggc gaggacggtc cgacccatca gccgatcgag catctcgcca
      541 tgctgcgcgc cacgccgaac ctgaccgtct tccgcccctg cgatgcggtg gaaaccgccg
      601 aatgctggga actggcgctc gccgcccgca ggacaccgtc ggttctggcg ctgtcgcggc
      661 agaacctgcc ggtcctgcgc acggaagcag gcgcgaaccg ctcggagcgt ggcgcctatg
      721 tgctgcgcgc ctgcggtgac aagcgcgaca ttaccctgct cgcctccggc tcggaagtcg
      781 aaatcgcctg cgccgcagcc gatacactga cggcgggcgg ccttcaggtc gccgtcgtct
      841 ccatgccgtc ctgggagctc tttgaggcgc aggacgaagc ctatcgccgt gcggttctcg
      901 gcacagctcc acgcatcggc atcgaggccg cctgccgcct cggctgggac cgctggatcg
      961 gcgaacgcgg catcttcatc ggcatgaccg gattcggcgc cagcgcgcct gccgccgatc
     1021 tctaccggca tttcggcatc acgccggccg ccattgtcga cgccgccgac aagctgctcg
     1081 gctaacggaa ttaaaggaaa ccaccatggc tcgcatcacg ctccgccaat tgctcgatca
     1141 cgcagccgaa cgcggttacg gcgtgccggc attcaacatc aacaacatgg agcaggtgct
     1201 cgccatcatg gaggcggcga aggccgtcga cgctccggtg atcctgcaag cctcgcgcgg
     1261 cgcgcgctca tatgccaacg acatcatgct ggcggccatg gtggacgcgg ctgagcgcat
     1321 gttccccgac attccgatct gcctccatct cgaccacgga aacgaggagg cgacttgcgc
     1381 caccgcgatc cagcatggct tcacctcggt gatgatggac ggctcgctgg aagccgacgg
     1441 caagacgccg gccgactacg cctacaacct ggcgatcacc cgccgcgtga ccgacatggc
     1501 gcattgggtc ggcgcctcgg tcgagggcga actcggcgtg ctcggctcgc tggagcatgg
     1561 cacgggcgag caggaggatg ggcacggcgc ggagggcaag ctctcgcacg accagctgct
     1621 caccgatccg gaccaagcgg tggatttcgt ctcgcgcacg aaggtcgatg cgcttgccat
     1681 cgccatgggc acctcgcacg gcgcctacaa gttctcgcgc aaaccggacg gcgcgatcct
     1741 tgccatgcat gtgatcaggg cgatccacga gaagctgccg aacatccacc tggtgatgca
     1801 cggctcatcc tcggtgccgc aggacttgca ggccgaattc aacgcccacg gcggcgccat
     1861 gaaggcaacc tggggcgtgc cggtcgagga aatccagacc ggcatcaagt acggcgtgcg
     1921 caaggtgaac atcgacacgg actgccgcct cgccatgacg gcccagttcc gcaagatcgc
     1981 gcaggaaaac aaggcggagt tcgatccgcg caagttcctc aagcccgcga tggatgcgat
     2041 gcgcctgctc tgccgccagc gctacgagca attcggctcc gccggcaatg cctcgaagat
     2101 caaggtcgtg ccactcgcag agatggcgaa acgctaccgc tccggaagcc tcgatccgca
     2161 ggtcgccggc atgtctcagg ccgccgcgta atcacgttcg aagtcatttc tagacttcgt
     2221 tactttgaca aaaggaccca aacatgaacg tttccctcga cacctcggtc aaggagctaa
     2281 agggcgccga acgttacaag accggtgtca ttgaatacaa gcgcatgggc tactgggatg
     2341 ctgactatgt gcccaagccc actgacgtcc tcgcgctgtt ccgcatcacg ccgcaagatg
     2401 gcgtcgatcc ggtcgaggcc gccgccgcgg ttgcgggtga aagctcgaca gcgacctgga
     2461 cggtctgctg gaccgaccgt ctgaccgcca aggagaagta ccgcgccaag gccttcaggt
     2521 gcgagccggt gccgggctcg ccgggccagt acttcgccac gatcgcctat gacctcgacc
     2581 tgttcgagcc aggatcgatc gccaacctca ctgcatcgat catcggcaac gtgttcggct
     2641 tcaagccact caaggcgtta cgcctcgagg acatgcattt ccccgtcgct tatgtgaaaa
     2701 ccttcaaagg cccagcgacc ggcatcgtcg tggagcgcga gcgcctcgac aagttcggtc
     2761 gtccgctgct cggctcgacc gtgaagccaa aactcggtct atccggccgc aactacggcc
     2821 gcgtcgtcta tgagggtctc aagggcggcc tcgacttcat gaaggacgac gagaacatca
     2881 actcgcagcc cttcatgcac tggcgcgacc ggttcctcta ctgcgctgaa gctgtgaaca
     2941 aggcgtcagc cgccactggc gaggtcaagg gcacgtacat gaacgtgacc gccggaacga
     3001 tggaagagat gtatgagcgc gccgaattcg ccaaggagat cggatcctgc atcatcatga
     3061 tcgatctcgt gatcggctac acggccatcc agtccatgtc caactggtcg cgcaagaacg
     3121 acatgatcct gcacctgcat cgcgctggcc acggcaccta tacccgccag aaaacacacg
     3181 gcgtctcctt tcgcgtcatc gccaagtgga tgcggcttgc tggcgtggac catatccatg
     3241 ccggcaccgt cgtgggaaaa ctcgagggcg atcccaacac cacaaagggc ttctacgaca
     3301 tcctgcgtca ggattacgtc cacaagaacc tctcgcatgg cgtgttcttc gaccagccgt
     3361 gggcgagtct caaccagtgc atgccggttg cctccggtgg tattcatgcc ggccagatgc
     3421 accagttgct tgatcttctt ggcgaagaca ccgtgctgca gttcggcggc ggcacgatcg
     3481 gccatccgca gggcatccag gccggcgcca cggccaatcg cgttgctctt gaagccatgg
     3541 ttctcgcccg caacgaggga cgcgacatcg tcaacgaagg tccgcagatc ctgatggacg
     3601 ccgccaaatg gtgtgcgcca ctgaagcagg cgctcgatac ctggaaggat atcactttca
     3661 actacgagtc gaccgatgct ccggacatgg tgacgactgc cacaccgggt cggtgatcag
     3721 ccacctacat cgaatgcact caaattcgac cggattcgaa aaggatcagc tccaatggca
     3781 atgaccaaca ctggaaagag aataacgcag ggacaatttt cgttccttcc tgatctgacg
     3841 gacaagcaga tcacggccca aatcaaatat ggcctcaaga acggctgggc gtggagcgtt
     3901 gagtacaccg atgatccaca tcccagaaac acctactggg agatgttcgg catgccgatg
     3961 ttcgacctca aggacgccgc cggcgtgctt tcggaagtga acagctgtcg caagacgttc
     4021 ccaaaccatt acgttcgtct cacggcgttt gacagctcac atggctggga gtcaccgcgg
     4081 atgtccttca tcgtcaaccg accgaaggac gaacccgggt tccttgtcga gcgtcaggaa
     4141 cgcaacggcc gggcgcaggg ctacgctatc cgatcctacg cgacgaacaa gccggagagc
     4201 gagcgctact cctgaccggg ctgacccagg ctgtctcggt tccagagcat ttttcaagcg
     4261 agatggttac cggttcgcgt gaagaaaatg cgacagaaca aacagataga gcgttcgaag
     4321 tgaatgtgaa ttctctggga gcgccctagc ccgggtcttg tcgcctgcat cagcccacgc
     4381 cttgtaacca gcgcctcccc agcccgcggc tccccgacgc ccggtgctcg tgctggttac
     4441 ctctgggcgg cggagtgcca tcgtcaccat tccgccgccc ttttccattc acagcgaggc
     4501 attcttgatg tctgcagacc ccgcaacgca gatcgacctg aaggccgagt tcgcccaaac
     4561 gcagatcggc gaagttctgg acgcgctcga tcgggagttg atcggtctga agccggtcaa
     4621 actgcgcatc cggcagatcg ctgccctgct gctcgtcgat cgcgtgcgcc gcaagctcgg
     4681 aatgacgtcc ggcgcgccct cactgcacat gagcttcacc ggcaatccag gcacgggcaa
     4741 gaccaccgtt gcgctgcgca tggccgaaat cctgcataaa ctggggtatg tccgggaagg
     4801 tcacctcata gccgtcacga gggatgacct cgtggggcag tacatcggcc atactgcgcc
     4861 aaaaacaaag gaagtgctca agcgcgccat gggcggcgtt ctgttcatcg atgaggccta
     4921 ttacctctac cggccggaga acgagcgcga ctacgggcag gaggcgatcg aaatcctgct
     4981 ccaggtcatg gagaaccagc gcgaagacct cgtcgtcatc ctcgccggct acgccgagcg
     5041 catggagacg ttcttccggt cgaacccggg ttttcgctcc cgcgtcgcgc atcacatcga
     5101 ctttccggac tatgagagcg cagagcttct cgacatcgcc gacacgatgc ttgcggcaca
     5161 gaactaccgc ttctcgaaac cggggcgcgc ggccttcgcc gactacatcg ctgcgcgccg
     5221 ggagcagccg catttcgcca acgcccgctc catccgcaat gccctggacc gggcgcggct
     5281 gcggcaggcg agccggcatt tcgaatccgg caaagcggtc aacctcgacg acctgatgac
     5341 catcgatgca gccgacatcc ttgccagccg cgtcctgacg cagaacggcg ccctgacccc
     5401 tgccgcccgc gagtgaagga gatcgccatg ccccgtccgc tcaagatcgc accgtcgatc
     5461 ctctccgctg actttggcga cctgcgcgcc gatgtcgccg ccatgatggc ggccggcgcc
     5521 gactgggtac acatcgatgt gatggacggg catttcgtgc ccaacatctc gttcggcgcg
     5581 ccgatcatca aggcgctcag gcctgcgacg ccggcgctgt tcgacacgca tctgatgatc
     5641 gcgccggtcg acgcctacct caaggatttc gccgacgccg gtgccgatct catctcggtg
     5701 cacgtcgagg ccggcccgca tatccaccga tcattgcagg caatccgcgc gttaggcaag
     5761 aagtcgggcg tcgtcgtctg tcctggaacg ccggccagcg ccgtcaggcc actgctcgac
     5821 gtggtggatc tggtcctgtg catgagcgtc aaccccggct tcggcggcca gagcttcatc
     5881 ccctctgtgc tggagacgat ccgcgatatg cgggcgctga tcgcagaccg gccgatcgat
     5941 cttgaggttg atggcggcgt gacgccggaa aacgccaggg tggtcgtcga ggcgggcgcg
     6001 aacgtgctcg tcgcaggctc tgcggccttc aggggcgggc gcagcgccta cgccgccaat
     6061 gtcgcggcga tccgcaaggc agccacggca tgacatggtc cgcacgtcgg ggcagcaccc
     6121 tgcccatcga agcgttgatc ttcgatgtcg atggcaccct ggcggaaacg gaagaattcc
     6181 accgccgagc tttcaacgcg gctttcacga cccttgggct cgactggttc tgggacaggc
     6241 ccctctatgc ggaccttctg aaagtcaccg gcggacgcga gcgcatccgg catttcatgg
     6301 atcactatct gtctggcacc ggcgaggcgc gggacatggt tgcgctgctc catcagcgca
     6361 agaacagggt ctatgccggg ttcgtcgcca accgtcaggt gccgctgcgg ccgggcatgc
     6421 gcgaactgat cgccgccgcg caaggcgcgg gcctgaagct ggccatcgcg acaacgacca
     6481 gccccgcgaa tgtcgacgcg ctcctgacgg cggcgctcgg ctccggctgg cgcgaggtct
     6541 tcccggtgat ggtggccggc gacatggtga aagccaagaa gcccgcgccg gatgtctacc
     6601 acgccgcgct ggcgcgactg cgcgttgcgc ctgaggcagc gctcgccatt gaggattccc
     6661 gcaacggtgt gctgtctgcg acgggggctg gcctgagggt ggcggcggtc cgcagcgcct
     6721 acacggcaca ggacgacctt tccggtgcgg tcagggtgtt cgacgatccg gtcgacctca
     6781 cgctggcgag cctgcgcgcg ctggcctgac gccagttcgg ctgcgtaacg gcggcaggtc
     6841 tgcttctcgc gacccgctct tctgcgcgat gcatgcggcg cgagatgcgg gcctcttgcc
     6901 agcaggcgtg acagacgatc atcatcggtc acttctcgtg accgtcggta tcaggtccgc
     6961 agcacaacct tggcggcggc gatttgattg gcgtcgatat cgtgaaacgc cctggcgcca
     7021 tcggcgaggt cgcgctgcgc gaaccaggcg aggctcccca attgccgcgc agccagggcc
     7081 tgcacaacct cgccgaagtc gcgcgcggtg tagcagtagg atcccaggaa ggtgacttcc
     7141 tgcaaggtca ccttgcgcac gtcgagcccg tcgagcccgg gcagcagccc gagatggacg
     7201 atgaccccgc cgggtcgcac caggcggctc gccgccgcac gggttgccgc ggcaccgacc
     7261 gcatcgatga caagatcgac gctgttctcc gccggctcgt ccggcgtgcc gggcgcatag
     7321 gcgctgaaac cgcctgtctc gcgcagggtc cgcagtctca ggccattggt ctcgccgaca
     7381 ccaagatcct gcgccccgaa gtggcgcgcc accagcgcgc aggcaaagcc gatcgcgccg
     7441 ccgcccaggg agatgatcat cgcctcggcc aggaactgcc g
//
LOCUS       DAOMXN010000125         7355 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_5258_length_7355_cov_11.521370, whole genome shotgun sequence.
ACCESSION   DAOMXN010000125 DAOMXN010000000
VERSION     DAOMXN010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 7355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7355
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5258_length_7355_cov_11.521370"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..729)
                     /locus_tag="PK812_09280"
     CDS             complement(106..729)
                     /locus_tag="PK812_09280"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HRJ69789.1"
                     /translation="MKATPRRTRAKDGMPSSAAGVNSAVGRTMRELRELRGITARDLA
                     ARASISAAMVSRIESGQVSPSLATLEMIATALEVPFPSIFRDIGPTVSDFTLVRGGQG
                     LKSKRQMGRHAHEFAVLGFHRRPDLQFECLLVTLNRNEEATPPTYTGHGCVFVYMLEG
                     EAIYRYGQEEIHLREGDSISLDAELRYGIQKVLTPRLRFLSVQAERR"
     gene            complement(765..1850)
                     /locus_tag="PK812_09285"
     CDS             complement(765..1850)
                     /locus_tag="PK812_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69790.1"
                     /translation="MTFDQRSFERGARNMLVGCAGAKPGEKLLIVREEPGAGYHCDEL
                     PEALAATAGEMGLHVGVVEELFRPEATALSPELEARVRDFDHVVFLARIGDQLRFRPM
                     PGGPSLTVNYALDGEALASAFGTAPHAALLDLKTAFNLLLHSAGKIRVTCPLGTDFSG
                     AAPAPKGSAPADVTIRRFPMSVFAPLDASGFSGRVAIAHLLVGTGSNYYEPYGIPVAA
                     PIIAVMEKGRLLRFEGDAEGVARAEAHYDRVSGLLGLDAHFVHSWHAGIHPGCRFTGS
                     AHDRYERWSGSAFGNPRLLHFHTCGDYAPGEISWNVVDPTIEADGVTIWRDGRILIDN
                     VPGAGEVLDKYQELRDLFERPERHIGL"
     gene            2012..3046
                     /locus_tag="PK812_09290"
     CDS             2012..3046
                     /locus_tag="PK812_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013530418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HRJ69791.1"
                     /translation="MTQYLKDCWYVAGWSKDVASRLTHVRLLGEDIVLYRTAAGAVAA
                     LEDACPHRKLPLSKGTLKDDRVVCGYHGLTFDARGACVAAPTQDRVPTGAKVRSYPAV
                     ERYGFVWLWMGAPERADETLVFDVPNYGAPGWGRTEGGDMPIACNYLYITDNLLDPSH
                     VAWVHLTSFAGAKTESEPLKIDVVDNGVIVWRWMMDTAPPAYYAPLLKFSGNVDRKQH
                     YECVIPSIGVNKSIFAPAGHGGPDKPLHEKTFVNISYNFMTPIDEDNTRYYWFQHRNT
                     NPDDAEVSAWMNKGAYTAFTEDKDVLEAVHLGMKNRRTPYLDLGLDAGALRFRKLLAK
                     RMEAEAARGA"
     gene            3054..4127
                     /locus_tag="PK812_09295"
     CDS             3054..4127
                     /locus_tag="PK812_09295"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRJ69792.1"
                     /translation="MTGAGKSWRRLRVARREQECAGVVSLHLAPADGSALPAFLPGQF
                     LTFRVPDDAGGFTPRNYSLSSDPEARSHWRVSVKRHPGGLGSGHMHVAMQVGDEIDVL
                     GPKGEFTLRAGSSRPVILLAGGIGITPLLAMAHALARDGRRPATLIHACESGAVQPFR
                     RELGVLAMGSSCIRIATCLANPTPADKAERAHQFEGLVDARVLRSLLPIGDYEAYLCG
                     PPGFMQAMYDLLIELGVREENIAYEFFGPARRLTHAPAPSAPAAPQPVPQDLVASGQT
                     IVRFTRSGREAVWDGAHRTLLELAEAQGLQPAFSCRNGICNTCLCAVEGPVHYVEEPL
                     DMPENGHALLCCAVPDGPVTVDL"
     gene            4129..4980
                     /locus_tag="PK812_09300"
     CDS             4129..4980
                     /locus_tag="PK812_09300"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRJ69793.1"
                     /translation="MTTDVTLPAPPSSAPSIRDQRRAKARRALIIDLVSFAAFVAVLG
                     WIILRGAQNMGYNWQWFRVWRYLWRNIDGEIIWGPLMKGLFVTVQISALAMVLTLVIG
                     LCTALLRLGTSPVGRAIARVYLEVIRNTPILVQILVFYFIIAHILGISRFWAGVLCLS
                     FYEGAFASEIIRGGIIAVKRGQWEAARSLGLTAPQVYRDVVLPQAVPLMLPPLAGVLV
                     NLVKHSSIVSVIAIFDLTTQGRTVVADTFMAFEIWLLVAALYLMVTLTLSLLVLGLER
                     RLGFTQH"
     gene            5014..5853
                     /locus_tag="PK812_09305"
     CDS             5014..5853
                     /locus_tag="PK812_09305"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRJ69794.1"
                     /translation="MSVWKSLKCTLAGALAVFAVGMLTAAPVAAQQPAAPANLIDTIK
                     RRNKLIVGMASFVPWAMRNTKDEWIGFEIDVAKKLADDMQVELELVPTAWDGVIPALL
                     SSRFDMIIGGLSITAPRALQVNFTIPYSQSGTGVAANKALTANMKWPADYNDPKVTFT
                     CRRGVLACRWIENTFPKATLRQFDDTAVQLQEVINGNAHATMGSEPLATFAALQNPDK
                     IVKPHEGYITTSVEGFGLRKGDPDALAFLNAWIAANQASGWLKGRHDYWFRTRNWADQ
                     VKQ"
     gene            5873..6799
                     /locus_tag="PK812_09310"
     CDS             5873..6799
                     /locus_tag="PK812_09310"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRJ69795.1"
                     /translation="MTGGSSATDVRPPPVKRRRQAFGWVDFVLAGLVAAFIAYFVWRT
                     NSVLHYRWNWGSVWPWVIRYDAQTETWIPGAILGGLATTLRLAVFGLVLAAIIGVVMG
                     FMRVSKRLLPRMISTSYVMLIRNIPPLVFVFVFVFFITSQILPSLGIADAARGLSPTT
                     QWWLSWFFGPVSRIENFFVGLICLAVFTGAYVTEIVRAGIESVPKSQIEAGESLGLTR
                     LQVMRDVVFPQAMRNVVPPMAGQFIQMIKDSSLVSLVSVQELTFVAQDIQTSAQRVFE
                     ILLFVGGVYFVICWTLSMIFGRLEHTASKERR"
     gene            6801..>7355
                     /locus_tag="PK812_09315"
     CDS             6801..>7355
                     /locus_tag="PK812_09315"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HRJ69796.1"
                     /translation="MSAPPIACSIDLTAPGKRIGHLRLSWSDNERAYGVIPVPIAVIK
                     GGDGPTALVTSGVHGDEYEGLLIARRLIADMQPKDLAGRLIVMPGVNWPAVEARTRTS
                     PIDLQNMNRAFPGEVRGGPTAMIADFIERVLLPQVNVAADLHSGGTKSIYTPCGYVYG
                     MGDRGFRERKLAAAHAFGAPATAVV"
BASE COUNT         1270 a         2391 c         2307 g         1387 t
ORIGIN      
        1 gtgtcgcggc gacggcggcg ccggccaaca ggagggactt cgcaagatca cgtctcttca
       61 tggatcaggc tccttgctga aagagaaatg gagtgccggg cggcgtcatc gacgctcggc
      121 ctggacggac aggaaacgca gtctgggcgt gaggactttc tggatgccat agcgcagttc
      181 ggcatctagg ctgatggagt caccctcgcg caaatgaatt tcctcctgcc cgtaacgata
      241 gatggcttcc ccctcaagca tatagacaaa aacgcagcca tggcccgtat aggtcggggg
      301 tgtcgcttcc tcattgcggt tcagggttac gagaaggcac tcgaactgca ggtcgggccg
      361 gcggtggaac ccgagcaccg cgaactcgtg ggcgtgacgc cccatttgcc gcttcgactt
      421 cagcccctgc ccgccgcgca ccagcgtgaa gtccgacacc gttggcccta tatcgcgaaa
      481 gatgctggga aagggaacct ccagtgccgt cgcgatcatc tccaacgtgg cgagcgaagg
      541 cgatacctga ccggactcga tgcgcgaaac catcgctgcc gagatcgacg cgcgcgcagc
      601 cagatcgcgg gcggtgattc cccgaagctc tctcagctcg cgcatggtgc gtccgaccgc
      661 cgaattcacg ccggctgctg aagacggcat cccatccttt gcccgcgtgc ggcggggcgt
      721 cgccttcatc tgccgagcct ccccccggag ccttgccgtg ccgattacaa gccgatgtgt
      781 cgttccggcc gttcaaaaag gtcgcgtaac tcttggtact tgtccagcac ttctcctgcc
      841 cccggcacat tgtcgatcag gatgcgcccg tccctccaga tggtgacgcc atccgcctct
      901 atcgtgggat cgacaacgtt ccaggagatt tcgccgggag cgtagtcgcc gcaggtatgg
      961 aaatggagca gccgcggatt gccgaacgcc gagccgctcc agcgttcgta cctgtcatgc
     1021 gccgagcctg tgaaacggca gccgggatga attccggcgt gccaggagtg cacgaaatgc
     1081 gcatccagcc ccaggagacc ggaaaccctg tcgtaatgcg cttctgcgcg ggcaacgccc
     1141 tcggcgtctc cctcgaaccg cagcaaccgg cccttctcca tcaccgcgat gatcggcgcg
     1201 gcaaccggga ttccatacgg ttcgtagtag ttcgatcccg tgcccaccag caaatgcgct
     1261 atggcgacgc ggcccgaaaa gccgctcgca tccagcggcg cgaagaccga catgggaaaa
     1321 cgccggatcg tcacgtcggc cggcgctgat cctttcggcg ccggggcggc tccggagaag
     1381 tccgtgccca gcgggcacgt tacgcggatt tttcccgccg agtgcagcaa caggttgaag
     1441 gcggtcttga gatccagcag agcggcgtgc ggcgccgtgc cgaaggcgga agccagcgct
     1501 tctccgtcca gggcatagtt cacggtcagg ctcggcccac cgggcatcgg ccggaaccgc
     1561 aactgatcac cgatccgggc caggaacacg acatggtcga agtccctgac cctggcctcg
     1621 agttccggtg acagcgcggt cgcttcgggt cgaaagagct cctcgacgac gccgacatgc
     1681 agacccattt ctcctgccgt cgccgcgagc gcttccggca gttcgtcgca gtggtaaccc
     1741 gcgcccggtt cctcgcgaac gatcagcagc ttttcgcccg gtttcgcacc cgcgcatccg
     1801 accagcatgt tccgcgcgcc gcgctcgaag ctgcgctgat cgaaggtcat cccggacccc
     1861 tcccaaagtc gccatcatcg ttgacgttca gttatgcaca tgtcacattt actgtcgatg
     1921 aatttatcga tcaaagcgca accgtgagag cgcgtcttcg atacagtggc gcgtgcgctg
     1981 atcctgtcgc cggtcaccta gggggaggac gatgacgcag tacctgaaag attgctggta
     2041 cgtggccggt tggtccaagg atgtcgcgtc gcgcctgacg catgtgcgcc tccttggcga
     2101 agacattgtt ctgtaccgga ccgccgccgg cgccgtcgcc gcgctggagg atgcgtgccc
     2161 gcacaggaaa ctgccgctct ccaagggaac gctgaaggac gaccgcgtgg tctgtggcta
     2221 tcacggcctc acattcgacg cgcgcggcgc ctgtgtcgcg gcgccgacgc aggacagggt
     2281 tccgaccggc gccaaggtca ggagctatcc ggcggtcgaa cgctacggct tcgtctggct
     2341 ctggatgggc gccccggaac gcgccgacga aacccttgtg ttcgacgtgc cgaattacgg
     2401 cgcgccgggc tggggccgga ccgagggcgg cgacatgccg atcgcctgca actatctgta
     2461 catcaccgac aaccttctgg acccctcgca tgtcgcctgg gtgcatctga cctcatttgc
     2521 gggcgccaag acggagtccg agccgctcaa gatcgatgtc gtcgacaacg gcgtcattgt
     2581 ctggcgctgg atgatggata cggcgccgcc ggcctattat gcgccgctgc tgaagttttc
     2641 cggcaatgtt gaccgcaagc agcactacga gtgcgttatt ccctcgatcg gcgtcaataa
     2701 gtcgatcttc gcgccggcgg gtcacggcgg accggacaag cccctgcacg agaagacgtt
     2761 cgtcaacatc tcgtacaact tcatgacgcc gatcgacgaa gacaacaccc gttactactg
     2821 gttccagcat cgcaacacca accccgacga tgcggaagtt tcggcctgga tgaacaaggg
     2881 tgcctatacg gcttttaccg aagacaagga tgtgctggag gcagtgcatc ttggcatgaa
     2941 gaaccgccgg acgccctacc tggaccttgg actggatgcc ggcgcgctgc gcttccgcaa
     3001 gctcttggcc aagcgcatgg aagccgaagc ggcccgggga gcctgatgcc gtcatgaccg
     3061 gggccgggaa aagctggcgg cgattgcgcg tcgcgcggcg cgaacaggag tgcgcgggcg
     3121 tcgtctcgct gcatctggcg ccagctgacg gcagcgccct gccggctttt cttcccggcc
     3181 agttcctgac ctttcgggtg ccggacgacg caggcggctt taccccgcgc aactacagcc
     3241 tttcctccga tccggaggcg cgctcgcact ggcgggtcag cgtcaagcgg catcccggcg
     3301 gcctcggatc tggtcacatg cacgtggcga tgcaagtcgg cgacgaaatc gatgtgctcg
     3361 gtccgaaagg ggagttcacc ctgcgcgcgg gatcgtcccg cccggtcatc ctgcttgcgg
     3421 gcggcatcgg tatcacgccg ctgctggcca tggcccatgc gctggcgcgg gatggccggc
     3481 ggcctgccac cctgatccat gcctgcgaaa gcggtgcggt tcagccgttc cggcgcgaac
     3541 tcggtgttct ggccatggga tcatcctgca tccggatcgc gacctgcctt gccaatccga
     3601 ccccggccga caaggcggag cgcgcgcatc agttcgaagg cctcgtggat gcccgggtcc
     3661 tgcgctctct gctgccgatc ggggactacg aggcctatct gtgcggtcct ccaggcttca
     3721 tgcaggccat gtatgatctc ctgatcgaac tcggggttcg cgaggagaac atcgcctacg
     3781 agttcttcgg cccggcgcgg cgcctcacgc atgcgcccgc gccttccgca ccggctgcgc
     3841 cccagccggt gccacaagac ctggtcgcaa gcggacagac catcgttcgc ttcacgcgtt
     3901 caggacgcga ggccgtttgg gacggagcgc accgaacctt gctggagctg gccgaggcgc
     3961 agggcctgca accggccttt tcctgccgga acgggatatg caatacttgc ctgtgcgcgg
     4021 tcgaagggcc ggtgcattat gtcgaggagc ccctggacat gccggaaaac ggccacgcgc
     4081 tgctctgctg cgccgttcca gacggtccgg taactgtgga cctatgagat gacgacggac
     4141 gtcacgctcc ccgctccacc gtcgtcggcc ccctccatcc gggaccaacg ccgggcaaag
     4201 gcgcgccgcg cgctcatcat cgacctggtt tccttcgcgg ccttcgtcgc ggtgctgggc
     4261 tggatcatcc tgcgcggcgc gcagaacatg ggctacaact ggcagtggtt tcgggtctgg
     4321 cgctacctgt ggcggaacat tgacggcgag atcatctggg gtccgctgat gaaggggctg
     4381 ttcgttaccg tccagatctc ggcgctggcc atggtgctca ccctcgtcat agggctgtgt
     4441 accgcgctgc tgcgccttgg caccagcccc gtaggccggg ccatcgcacg ggtctatctc
     4501 gaagtgatcc gcaacacgcc catcctggtg cagatcctcg tcttctactt catcatcgcc
     4561 catatcctcg gcatttcacg gttctgggcg ggcgtgctgt gcctgtcctt ctacgaaggc
     4621 gcctttgcca gcgaaatcat ccgaggcggg atcatcgcgg tcaaacgcgg gcaatgggaa
     4681 gcggcgcggt ctctcgggct gacggcgccg caggtctatc gggacgtcgt cctgcctcag
     4741 gccgtgcccc tgatgctgcc cccactggca ggcgtcctgg tcaacctcgt gaagcattcg
     4801 tccatcgtca gcgtgattgc gatcttcgac ctgaccacgc agggccgcac cgttgtcgcc
     4861 gacacgttca tggctttcga aatctggctc ctggtcgccg cactgtatct gatggtcacg
     4921 ctcaccttat cgctcctggt gctgggcctg gagcgacgcc tcgggttcac ccagcattag
     4981 ccgcgcggtt tgcgcatttg gagggaggat tacatgtcgg tctggaagtc actcaagtgc
     5041 acgctggcgg gagcgctggc ggttttcgcc gtcggaatgc tgacagcagc accggtcgcg
     5101 gcgcagcaac ccgcggcgcc ggccaacctg atcgatacga tcaagcgtcg gaacaagctg
     5161 attgtcggaa tggccagttt cgtgccttgg gccatgcgca acacgaagga tgagtggatc
     5221 ggcttcgaga tcgatgtcgc caagaaactc gccgacgaca tgcaggtgga actggaactc
     5281 gtgccgaccg catgggatgg cgttattccg gccctgctct ccagccgctt cgatatgatc
     5341 atcggcggcc tgagcatcac cgcgccacgt gcgctccagg tgaatttcac gatcccctac
     5401 tcgcaatccg gcacgggcgt ggccgccaac aaggctctga ccgcgaacat gaagtggccc
     5461 gctgactaca acgatccgaa ggtcaccttc acctgccgtc gcggcgtcct ggcgtgtcgc
     5521 tggatcgaaa acacgttccc gaaagcgacc ctgagacagt ttgacgacac cgccgtgcaa
     5581 ctgcaggaag tcatcaacgg caacgcccat gcgaccatgg gcagcgagcc gctggcgacc
     5641 tttgcggcgc tccagaaccc ggacaagatt gtcaagccgc acgagggtta catcaccacg
     5701 tccgtggaag ggttcggact gcgcaagggc gatccggacg ctctcgcctt cctcaacgcc
     5761 tggatcgcag ccaaccaggc atccggctgg ctgaaagggc ggcacgacta ctggttccgc
     5821 actcggaact gggccgacca ggtcaagcag taagatctga cggacggcat ccgtgacagg
     5881 ggggagttcg gcgaccgatg tgcggccgcc gccagtaaag cggcggcggc aggcgttcgg
     5941 ctgggttgac tttgtgctgg ccggccttgt cgccgccttc atcgcgtatt tcgtctggcg
     6001 caccaatagc gtgctgcact accgctggaa ctggggcagc gtctggccct gggtcatccg
     6061 gtatgatgcg cagaccgaga cctggattcc gggcgcgatc cttggcggtc tggccacgac
     6121 cttgcggctc gccgtgttcg gcctcgttct ggcggcgatc atcggcgttg tcatgggctt
     6181 catgcgcgtg agcaaacgcc tgttgccgcg catgatttcg acctcttacg tgatgctcat
     6241 tcgcaatatt ccgcctctcg tttttgtttt tgtcttcgta ttcttcatca ccagccagat
     6301 cctgccctcg ctcggcatcg ccgacgcggc gcgcggactg tcgccgacga cgcaatggtg
     6361 gctttcctgg ttcttcggac cggtttcaag aatcgagaac ttcttcgttg ggctgatttg
     6421 tctggccgtc ttcaccgggg cctatgtcac cgagatcgtg cgcgccggca tcgaatccgt
     6481 gccgaagtcg cagatcgagg ccggcgaaag ccttggcctc acgcgtctcc aggtgatgcg
     6541 cgacgtcgtg tttccgcagg ccatgcgcaa cgtggtgccg cccatggcgg ggcagttcat
     6601 ccagatgatc aaggactcct cgctggtatc tctggtttcg gtccaggaac tgaccttcgt
     6661 cgcgcaggac attcagacct cggcacaacg tgtatttgaa atcctgctct tcgtgggcgg
     6721 ggtctacttc gtcatctgct ggaccctgtc gatgattttc gggcgcctgg agcacaccgc
     6781 atcgaaggaa cgccgctgac atgtccgcac cgccgattgc ctgcagcatc gacctgaccg
     6841 cgcccggcaa gcggatcggc catctgcgcc tgtcctggtc ggacaatgag cgggcctatg
     6901 gagtaatccc ggtcccgatc gccgtgatca agggaggcga tggtccgacg gcgcttgtca
     6961 cctcgggcgt gcacggcgac gaatacgaag gcctgctcat cgcccgccgt ctgatcgcgg
     7021 acatgcagcc gaaggacctc gcggggcgtc tgatcgtcat gcccggcgtc aactggccgg
     7081 ccgtggaggc ccggacccgc acgtcgccca ttgacctgca aaacatgaac cgggccttcc
     7141 cgggcgaagt caggggcggc cccacggcga tgatcgccga cttcatcgag cgggtgctgc
     7201 ttccacaggt gaatgtggca gcggacctgc attccggcgg aaccaaatcg atctacacgc
     7261 cctgcggcta tgtctatggc atgggcgacc gcgggttccg cgagcgcaag ctggccgcgg
     7321 cgcatgcgtt cggcgcgccg gcgacggccg tggtg
//
LOCUS       DAOMXN010000126         7323 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_5294_length_7323_cov_13.851403, whole genome shotgun sequence.
ACCESSION   DAOMXN010000126 DAOMXN010000000
VERSION     DAOMXN010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 7323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7323
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5294_length_7323_cov_13.851403"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..943)
                     /locus_tag="PK812_09320"
     CDS             complement(83..943)
                     /locus_tag="PK812_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017961877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRJ69797.1"
                     /translation="MESRTESISSPLRNVSLEERAYRIRRNALRMGEVQGQGYIAQAL
                     GVADVLAVSYFHALRYRPSEPEWEGRDRFCLSIGHYAIALYSALIEAGILPEDELESY
                     GCDDSRLPMSGMAAYTPGMEITGGSLGHGLGIAVGMALGLKRKNADSRVYNLFSDGEL
                     GEGSTWEAAITAASFKLDNLIGIVDVNNMQADGPAKEVTAFEPLGPKFEAFGWHVQRV
                     DGNDIPALVAAFDVAKTLKEPKPRVIICDTKMAKGVPFLEERERNHFLRVEADEWAKA
                     IAILDAGRPA"
     gene            complement(983..1735)
                     /locus_tag="PK812_09325"
     CDS             complement(983..1735)
                     /locus_tag="PK812_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003603845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRJ69798.1"
                     /translation="MLLKGKIAVITGAASKRGLGLAMAQLFAAHGARIAVLDLDLGGA
                     REAAHMLKGRGHIGLKCDVTRRKECDRAVQRVLARHGRIDILVNNAGITSPQRVMEVS
                     DETYSKVMDVNMRGTLHMTQAVVPTMRAQKAGSIVNLSSVSAQRGGGVFGGSHYAAAK
                     GAILGYTKACARELGPDNIRVNAICPSLILTDITGGALTPERMRDILATIPMGRAGEA
                     HEVAGCALFLASDLASYVTGSEIDINGGSHIH"
     gene            complement(1811..2581)
                     /locus_tag="PK812_09330"
     CDS             complement(1811..2581)
                     /locus_tag="PK812_09330"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HRJ69799.1"
                     /translation="MILPSDVLPHSGGNPDSLFSQVADKIAVAIVSGAIAAGELIPNE
                     EAIFGATTVSRSVYREAVKFLSGKGLIEARPKSGTRAAPVSNWNLLDPDVLRWSLSAG
                     PDEKFIRDLYELRGFIEPNVARLAALRRTDEQADALRAAYEGMASTPPATPENIRFDL
                     RFHEILMESCGNDALQRLKGVVMTTLMWAMGLQVGKKAVEYEPALGDHKRVMEAVANR
                     EGERAHAVMSVLVHDALYETLEKFRARKGQMAFRAAAE"
     gene            2800..3870
                     /gene="ugpC"
                     /locus_tag="PK812_09335"
     CDS             2800..3870
                     /gene="ugpC"
                     /locus_tag="PK812_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017967623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HRJ69800.1"
                     /translation="MASVEIRDVRKAFGAVQIIKGVNIDIGDGEFVVLVGPSGCGKST
                     LLRMIAGLENITGGEIAIGGRVVNQVPPKERDIAMVFQNYALYPHMTVKDNMAFSLKL
                     RKAPQSEVDERVGKAAAILGLGALLERYPRQLSGGQRQRVAMGRAIVRDPQVFLFDEP
                     LSNLDAKLRVQMRTEIKELHQRLKTTTVYVTHDQIEAMTMADKIVVMHDGIVEQIGAP
                     LDLYDKPANLFVAGFIGSPAMNFIKGSISGTEFSADGGFKLSYFSGPGDASDRKVILG
                     VRPEHIRLDDKGIPAEIVVVEPTGSETQVVLKIGEGEAVQEIIAVFRERMSERPGDII
                     TISPVISSSHLFDAASGRRISR"
     gene            3938..5257
                     /locus_tag="PK812_09340"
     CDS             3938..5257
                     /locus_tag="PK812_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRJ69801.1"
                     /translation="MTLNRRSLLAGAAGFGALGASGLAEFAAAWAQQAQWKPEANASL
                     QVLRWKRFIQAEEDGFMALVDAFTKATGVKVAVINESLDDVQPKASVAANTNQGPDIF
                     WGLYSLPHLFPEKCVDVTDVADYLGKKYGGWSPTAVTYGTSKGKWIALPIAYNANVIN
                     YRQSMIEKAGFKEIPKDTAGFLELMKGLKAKSVPGGFALGRASGDGNAWVHWCFWSHG
                     ANTVDKGDKVIINSPETAKALEYAKALYAEFAPGTASWNDAFNNKAFLSGEVSVTNNG
                     VSIYAAAKAGADKDPKMKEIMEDMNHSVWPIGPVGKPTEFHIAYPMMIMKYSKNQRAA
                     KAFMAFLMEAENYNKWLQSAVAYLSHPLKAYEANPVWTSDKKLAVARDAAWNTQTAGG
                     QGSVGERAASALADFVVLDMFAAVCTGREEVKGAIAIADRQLRRIYR"
     gene            5390..6331
                     /locus_tag="PK812_09345"
     CDS             5390..6331
                     /locus_tag="PK812_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRJ69802.1"
                     /translation="MAQSAAAPGGTMQPGLKERLATNRNWLGFWYMVPAMAFLVLFLA
                     YPLGLGVWLSFTDARIGRPGVFIGIENYEWVLGDRKFQDAVFNTLFYTFVASVAKFAI
                     GLYLAILLNKHIPFKAIVRAVILIPFIVPTVLSAIAFWWIFDPQFSIISWGLKKAGLI
                     TGNVDFLGDAWNARWSVIFANTWRGVPFIAITLLAGLQTVSPSLYEAATLDGASPWQM
                     FRHITYPLLTPIIAVVMTFSVLFTFTDFQLIKVLTNGGPAGATELMATMSYNTAILGG
                     KIGEGAAISTAMIPFLLGAIMISWFGLQRRAWQQGEN"
     gene            6331..7221
                     /locus_tag="PK812_09350"
     CDS             6331..7221
                     /locus_tag="PK812_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRJ69803.1"
                     /translation="MSASTSQPAADASEGMSYLDTLPRRIITVYVPLALIMIVLLFPF
                     YWMAITAIKPKEHLLDLETHNPLFTLSPTFEHIRALLFETEYPHWLWNTMYVSVAATI
                     LSLVASVLAAYAIVRIRYTGANLVGGLIFLAYLVPPSILFIPLSTVVYQYGLFDTQFA
                     LILTYPTILIPFSTWLLMGYFKTIPYELEECALIDGASRFEILTKIVLPLAIPGLISA
                     GIFCLTLCWNEFIYALTFINSQDKKTVPVAIVTAFTDGDQYRWGSLMAGALVGSLPLV
                     VLYAFFVEHYVSAMTGAVKE"
     gene            7227..>7323
                     /locus_tag="PK812_09355"
     CDS             7227..>7323
                     /locus_tag="PK812_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008934199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HRJ69804.1"
                     /translation="MASIAFVGLGAMGLPMADNLLKRQHKVRGFDM"
BASE COUNT         1328 a         2369 c         2274 g         1352 t
ORIGIN      
        1 atggaggcga tcatcgcgga tgtggtcaga cgcgtgccgt cggcggcgcg cggcaccttg
       61 cggggggtgt acttcgacag tctcatgctg gcctcccggc gtcgagaatg gcgatggcct
      121 tggcccattc atcggcctcg acgcgcagaa agtggttgcg ttcgcgctct tccaggaacg
      181 gcacgccctt cgccatcttc gtgtcgcaga tgatgacacg cggctttggc tccttcagcg
      241 ttttcgccac atcgaaggca gcaaccagcg ccggaatgtc gttgccgtcg acgcgctgca
      301 catgccagcc gaaggcttcg aacttcggcc cgagcggctc gaaagcggtc acctccttgg
      361 ccggcccatc cgcctgcatg ttgttgacgt cgacaatgcc gatcaggttg tcgagcttga
      421 aactcgcggc ggtgatcgcc gcctcccagg tcgatccctc gccaagctcc ccgtccgaga
      481 acaggttgta gacccggctg tccgcgttct tgcgcttgag ccccagcgcc atgccgacgg
      541 caatgcccag cccgtggccg agcgacccgc cggtaatctc catgcccggt gtgtaggccg
      601 ccatgccgga catcggcagg cggctgtcat cgcatccgta ggactccagc tcgtcctcag
      661 gcaggatgcc ggcctcgatc agcgcggaat agagcgcgat cgcgtaatgt ccgatggaca
      721 ggcagaagcg gtcgcgcccc tcccattccg gttccgaagg ccggtagcgc agcgcgtgga
      781 aataggagac ggccagcaca tcggcgacgc cgagcgcctg cgcgatgtag ccctgcccct
      841 gcacctcgcc catgcgcagc gcattgcggc gaatgcggta ggcgcgctcc tcaaggctga
      901 cgttgcgcaa gggggatgag atggattccg ttcgcgattc catggggtcg tgcctttggg
      961 acggacgtag cggcgcggcg cgtcagtgaa tatggctgcc gccgttgatg tcgatttcag
     1021 agccggtaac gtaggacgcc aggtccgaag cgaggaacag cgcacagcca gcgacttcgt
     1081 gcgcctcgcc ggcgcgaccc atcgggatcg tcgccaggat gtcgcgcatc cgctccgggg
     1141 tgagcgcgcc gccggtaatg tcggtgagaa tcagcgaagg gcagatggcg ttgacgcgga
     1201 tgttgtcggg gccaagctca cgggcgcagg ccttggtata gccaaggatc gcgcctttgg
     1261 cggcggcgta atgcgagcct ccgaagaccc cgccgccgcg ctgagccgag accgacgaca
     1321 gattgacgat cgagccggcc ttctgcgccc gcatcgtcgg gacgacggcc tgcgtcatgt
     1381 gcagcgtgcc gcgcatgttc acgtccatca ccttcgaata ggtctcgtcg gaaacctcca
     1441 tgacgcgctg cggcgaggtg atgcctgcgt tgttgacgag aatgtcgatg cggccgtgcc
     1501 gggcaagtac gcgctgcacg gcccggtcgc actccttgcg gcgggtgaca tcgcatttca
     1561 ggccgatgtg gccacggccc ttgagcatgt gcgcggcctc gcgcgccccg cccagatcca
     1621 ggtccagcac ggcgatcctc gcgccgtgcg cggcgaaaag ctgcgccatg gccaggccaa
     1681 ggccgcgttt tgacgcggcg cccgtgatga cagcgatctt gcctttcagc agcatgaatg
     1741 tctcctgtcc ccccaacgta cggtgtgcac ccgtatcgtt cgcgccgaat cgatcggcaa
     1801 ttaatatgat ttattccgct gcagcgcgga aggccatttg gcctttccgt gcccggaact
     1861 tttccagcgt ctcgtaaagc gcatcgtgaa ccagcacgct catcaccgcg tgcgcccgct
     1921 cgccctcgcg atttgccacc gcctccatga cacgcttgtg atcaccgagg gccggttcgt
     1981 actctacggc cttcttgccg acctggaggc ccatggccca catcaacgtg gtcatgacga
     2041 cgcccttcag acgctgcagc gcgtcattgc cgcaggattc catgaggatc tcatggaaac
     2101 gcaggtcaaa gcggatgttt tccggcgtcg ccggtggcgt cgacgccatg ccttcatagg
     2161 cggcgcgcaa cgcatcggct tgctcgtccg tgcgccgcaa ggccgccaaa cgcgcgacat
     2221 tcggctcgat gaagccgcga agctcataga ggtcgcggat gaatttctca tcgggtccgg
     2281 ccgagagcga ccagcgcaag acgtccggat cgaggaggtt ccagttcgag accggcgcgg
     2341 cgcgcgtgcc gctcttgggg cgcgcctcga tcagtccttt cccggacagg aactttaccg
     2401 cctcgcgata gacgctgcga gagaccgtcg tggcaccgaa aatggcctct tcgttcggga
     2461 tcagttctcc ggcggcgatg gcgccggaaa cgatggccac ggcgatcttg tcagcaacct
     2521 gcgaaaacag gctgtccgga ttgcccccgg aatggggaag gacgtcgctg ggcagaatca
     2581 cgcaagactc cctcggcgct gaccacggcc cgcgaagggc cgcatcggcg cttgacaact
     2641 gacatcatat aatatgactt attttgcgca agcgtgtttc aaggccttgt tcaagaggca
     2701 acgctggccg gcatgtccga catgctggaa catggcatcg gcgaagtccg tgcgtgtccc
     2761 ttgtgaccgc aaaacggtaa agccgaaggg gaggatggca tggcgtcggt agaaatccgc
     2821 gacgtccgca aggcgttcgg cgcggtgcag atcatcaagg gcgtgaacat cgacatcggc
     2881 gacggagagt ttgtcgtgct cgtcggtccg tccggttgcg gcaagtccac gctgctgcgc
     2941 atgatcgcgg gacttgaaaa catcaccggg ggcgaaatcg ccatcggggg gcgcgtggtg
     3001 aaccaggtgc cgccgaaaga gcgcgacatc gccatggtgt tccagaacta cgcactctac
     3061 ccgcacatga cggtgaagga caacatggcc ttctccctca agctgcggaa ggcgccacag
     3121 tcggaggtcg acgagcgcgt cggcaaggcc gccgccattc tcgggcttgg cgcgttgctg
     3181 gagcgctacc cccgccagct ctccggcggc cagcgccagc gcgtggcgat ggggcgcgcc
     3241 atcgtgcgtg atccgcaggt attcctgttc gacgagccgc tctcgaatct cgatgccaag
     3301 ctgcgcgtgc agatgcgaac cgaaatcaag gaactgcacc agcgcctcaa gaccaccacc
     3361 gtctatgtca cgcacgacca gatcgaggcc atgaccatgg ccgacaagat cgtggtgatg
     3421 cacgacggca tcgtcgagca gatcggcgcg ccgctcgatc tctacgacaa gccggccaac
     3481 ctgttcgtcg ccgggttcat tggttcgccc gcgatgaact tcatcaaggg atcgatctcc
     3541 ggaacggagt tttccgccga tggcggcttc aagctcagct atttttccgg gccgggcgat
     3601 gcttccgatc gcaaggtgat cctgggtgtc cggcccgaac acatccggct cgacgacaag
     3661 ggcattcctg ccgagatcgt cgtggtcgag ccgactggat cggaaacgca ggtcgtgctc
     3721 aagatcggag agggagaagc ggtgcaggag atcatcgccg tgttccgcga acgcatgagc
     3781 gaacggccgg gcgacatcat cacgatctcg cccgtgatct cgtcgtcgca ccttttcgat
     3841 gctgcaagcg gccggagaat ttcccgctga gcattttgaa cccctgcgga acgccccgtt
     3901 ccgcccaaca ccgagaagtg aaaccaggga ggataccatg acacttaacc gcagatcgct
     3961 gctggccggc gcggccgggt tcggagcgct tggcgcgtcc ggtcttgccg aattcgccgc
     4021 cgcctgggcg caacaggcgc aatggaagcc cgaggccaac gccagcctgc aggtgctgcg
     4081 ctggaagcgc ttcatccagg cggaggaaga cgggttcatg gcgctggtgg atgccttcac
     4141 caaggcaacc ggcgtcaagg tggcggtgat caacgagtcg ctggacgacg tgcagcccaa
     4201 ggcgtcggtt gcggccaaca ccaaccaggg tccggacatt ttctggggtc tctattcgct
     4261 gccgcacctg ttcccggaaa aatgcgtcga cgtcacggac gtcgccgact acctcggcaa
     4321 gaagtacggt ggctggtcgc cgaccgccgt cacctacggc accagcaagg gcaagtggat
     4381 cgccctgccg atcgcgtaca acgccaacgt catcaactat cgccagtcga tgatcgagaa
     4441 ggcgggcttc aaggaaatcc cgaaggacac ggccggcttc ctggagctca tgaagggcct
     4501 gaaggccaag tcggtccccg gaggcttcgc gctcggccgc gcctcgggcg acggcaacgc
     4561 ctgggtgcac tggtgcttct ggtcgcacgg ggccaacact gtcgacaagg gcgacaaggt
     4621 gatcatcaat tcaccggaga cagccaaggc cctggagtac gccaaggcgc tttacgccga
     4681 attcgccccg ggcaccgcgt cctggaacga cgccttcaac aacaaggcgt tcctctcggg
     4741 agaggtctcc gtcaccaaca acggcgtctc gatctatgcc gccgccaagg ccggcgcgga
     4801 caaggatccg aagatgaagg agatcatgga agacatgaac cattcggtct ggccgatcgg
     4861 tccggtcggc aagccgaccg agttccacat cgcctatccg atgatgatca tgaagtactc
     4921 caagaatcag cgggcggcca aggcgttcat ggcattcctg atggaggcgg agaactacaa
     4981 caagtggctg cagagcgcgg tcgcctacct gtcgcatccg ctcaaggcct atgaagccaa
     5041 cccggtctgg acgtcggaca agaagctggc ggttgcccgc gacgcggcct ggaacacgca
     5101 gacggcgggc ggtcagggct cggtcggcga gcgcgcggcc tcggcgcttg cggacttcgt
     5161 cgtcctggac atgttcgcgg cggtctgcac cgggcgcgaa gaagtcaagg gcgcgatcgc
     5221 catcgccgac cggcaattgc gtcgcatcta ccgctgagcg acggaaaccg gcgtggcgca
     5281 gatgcgccgc gccggtcgac acctccaaga ccacaacgac ggggcctccg gccccgccgc
     5341 gatctcatgg ccttcccccc tcgaaggcgg gacaatcagg acctgacgca tggcgcaatc
     5401 ggcagcggca cccggtggaa cgatgcagcc cggactgaag gaacggctcg cgaccaaccg
     5461 gaactggctc ggattctggt acatggtgcc cgccatggcc tttctggtgc tgtttctcgc
     5521 ttatcctctc ggccttggcg tctggctgtc cttcacggat gcacgcatcg gccgacccgg
     5581 cgtattcatc ggcatcgaga attatgaatg ggtgctgggc gacaggaagt tccaggatgc
     5641 ggtcttcaac acgctgttct acactttcgt tgcaagcgtc gcgaaattcg ccattggcct
     5701 ctatctggcg atcctgctga acaagcatat cccgttcaag gcgatcgtcc gtgccgtcat
     5761 cctgatcccg ttcatcgtgc cgaccgtgct ctccgccatc gccttctggt ggatctttga
     5821 cccgcagttc tcgatcatct cttggggcct gaagaaggcc gggctgatca cgggcaacgt
     5881 cgatttcctc ggcgatgcct ggaacgcccg ctggagcgtc atcttcgcca acacctggcg
     5941 tggcgtgccg ttcatcgcca ttaccttgct ggcgggcctg caaaccgtct cgccctcact
     6001 ctacgaggca gcgacgctcg acggcgcgtc gccctggcag atgttccgtc acatcaccta
     6061 tccgctgctg acccccatta tcgccgtggt gatgaccttc tcggtgctgt tcaccttcac
     6121 cgatttccag ctcatcaagg tgctcaccaa cggcgggccg gcaggcgcca cggaactgat
     6181 ggcgaccatg agctacaaca ccgccattct cggcgggaag atcggcgagg gcgcggcgat
     6241 ttccaccgcc atgatcccgt tcctgctcgg cgcgatcatg atttcctggt tcggcctgca
     6301 gcgccgcgcc tggcagcagg gagagaactg atgagcgcgt ccacctccca gcccgccgcc
     6361 gacgcctcgg agggcatgag ctatctcgac acgctgcccc ggcgaatcat cacggtctac
     6421 gtgccgctgg cgctgatcat gatcgtgctg ctgttcccct tctactggat ggcgatcacc
     6481 gccatcaagc cgaaggagca tctgctcgat ctcgagacgc acaacccgct gttcacgctc
     6541 agcccgacgt tcgagcacat cagggcactg ctgttcgaaa cggagtatcc gcactggctg
     6601 tggaacacga tgtatgtctc ggtggccgcg accatcctgt cgctggtcgc gtcggtgctg
     6661 gccgcctacg ccatcgtgcg catccgctac accggcgcca atctcgtcgg cgggctgatc
     6721 ttcctggcct atctggtgcc gccgtcgatc ctgttcatcc cgctctcgac cgtggtttac
     6781 cagtatgggc tgttcgacac gcagttcgcg ctgatcctga cctatccgac catcctcatt
     6841 ccgttctcga cctggctctt gatgggctac ttcaagacca ttccctatga gttggaggag
     6901 tgcgcgctga tcgacggggc gagccgcttc gaaatcctca ccaagatcgt gctgccgctg
     6961 gccattcccg ggctgatttc cgcgggcatc ttctgcctga ccctgtgctg gaacgagttc
     7021 atctacgcgc tgactttcat caattcccag gacaagaaaa ccgtgccggt ggcgatcgtc
     7081 accgccttca ccgacggcga ccagtaccgc tggggctcgc tgatggccgg cgcgctggtc
     7141 ggttcactgc cgctggtggt gctctacgcc ttcttcgtcg aacactacgt ctccgccatg
     7201 accggagcgg tgaaggagta ggcaccatgg ccagcatcgc cttcgtcggg ctcggcgcca
     7261 tgggcttgcc catggccgac aacctgttga aacgtcagca caaagtgcgc ggcttcgaca
     7321 tgc
//
LOCUS       DAOMXN010000127         7323 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_5295_length_7323_cov_12.174326, whole genome shotgun sequence.
ACCESSION   DAOMXN010000127 DAOMXN010000000
VERSION     DAOMXN010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 7323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7323
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5295_length_7323_cov_12.174326"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..1016
                     /locus_tag="PK812_09360"
     CDS             192..1016
                     /locus_tag="PK812_09360"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014328961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRJ69805.1"
                     /translation="MRPQCDSPLARLFASPNHGERKDGLRPDMIILHYTGMPTGDAAL
                     AWLCNPVSEVSSHYFVRENGRILQLVPEARRAWHAGASSWQGLGDINSRSIGIEIVNA
                     GHEGHGIDPVDPRRPPDVTAPDKGAGPLPAFPPAQIESVIRLVRDIAARWHIRPERIL
                     AHSDIAPRRKRDPGEAFPWPVLAENGVGHWVRPTPIRSGRFFQPGDGGAPVEALQQML
                     SLYGYDMPATGRYDEQTEAVVRAFQRHFRPERVDGIADFSTITTLRDLIRALPDGR"
     gene            1016..1588
                     /locus_tag="PK812_09365"
     CDS             1016..1588
                     /locus_tag="PK812_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69806.1"
                     /translation="MFVYPEDRKRLTGLAMQLLALAEQRFGPRVPGWTFLGVFFDSDG
                     PSIVFPTRARNELEMRVTFSCRNNADLAGFQIGHEVIHMLAPDRNPPASMLEEGIATL
                     FSLEGPSFLSPTYRTWSIHYLATHPQVHHYNEALGLVRELLAIQPDAVMALRRREPRI
                     HAFTAEFITQTLPRVPKSLAARLMDRRKMR"
     gene            1666..3927
                     /locus_tag="PK812_09370"
     CDS             1666..3927
                     /locus_tag="PK812_09370"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HRJ69807.1"
                     /translation="MTSLDVTQARGKHAGMTQARLVLIVYAVAIFASAFLLFSVQPMF
                     TRMVLPKLGGTPAVWSVAMVFFQTVLLIGYLYAHLSVTWLSARAASIVHLSIMGVAAL
                     GLPIAVSELLGDPPQTGAEFWLFAVFALSAGLPFFAIAGNGPLLQQWYHRAAGPSASN
                     PYTLYGASNLGSFGALLLYPFAFEPLFALKTQSHLWSIGFAMLAVVIALCGVLLVRAA
                     PGRVTTNQKDAAATAETPSSGRKLAWVFYAFVPSGYLVAVTAHISTDIASAPLLWIIP
                     LALFLLTFVAVFRDTPLLPPALFTRAWPIAFLLLLLPDWIEMPIAVSIALHVVGFFVA
                     TMVCHQALYASRPAASHLTLFYLLMSLGGVLGGVFASLVSPHVFSGVHEYLILMVLVF
                     LCLPGAMNAVRAHLAAILLALAGAAVLIVGMRSSLPVTLDPTAVALPVLVASVALFWT
                     ERHAPLRLAAIGGACLAVLAFAKDDGKVEHVTRSFFGVNKVMMIEGGKYRAIVHGNTN
                     HGAMRVLNDDGTPATGRQRPVAYYRDGAPYETAISRIRQRQGGLGSVAITGLGAGGLA
                     CHARDGEKWTFFEIDPEVIRIAQDTRWFRSLSDCTPDARIIAGDARLTLAKHPGGFDL
                     IALDAFSSDAIPVHLLTEEAIQLYFSKLNADGALIIHITNRYMELRSVVAAISARLNL
                     VGVARKLTQEEAMAESMSDMPVGVEVAVLARKKEDLGALPGLPGWDMLKADPSVASWS
                     DDYSNIVGALLRK"
     gene            complement(3937..>7323)
                     /gene="metH"
                     /locus_tag="PK812_09375"
     CDS             complement(3937..>7323)
                     /gene="metH"
                     /locus_tag="PK812_09375"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HRJ69808.1"
                     /translation="EAAALAQAKDGRRRFVAGAVGPTNRTASISPDVNNPGYRAVTFD
                     DLRIAYGEQIRGLIEGGAELILIETIFDTLNAKAAVFAAEEAFVATGVRLPLMISGTI
                     TDLSGRTLSGQTPTAFWYSLRHADPLSIGLNCALGAREMRAHIAEISKVADTLVCAYP
                     NAGLPNEFGLYDESPEAMARMIGDFASDGLVNIVGGCCGSTPAHIRAVADAMAGKTPR
                     RVPKAEPLLRLSGLEPFTLTKDIPFVNIGERTNVTGSARFRKLIKEGKYPEALDVARD
                     QVANGAQVIDINMDEGLLDSEAAMTTFVNLIAAEPDIARVPLMIDSSKWNVIEAGLKC
                     VQGKPIVNSISMKEGEEAFIHHAKLCRAYGAAVVVMAFDEQGQADSYERKIEICTRAY
                     KVLTEKVGFPPEDIIFDPNIFAVATGIEEHNGYGVAFIEATRTIRQSLPHCHVSGGVS
                     NLSFSFRGNEPVREAMHAVFLYHCIRVGMDMGIVNAGQLAVYDALPQELRDLCEDVVL
                     NRRPDATERLLDAAPRFKGDGKAEVKTADLEWRRLPVDKRLEHALVNGVTEFIEIDVE
                     EARAAAERPLHVIEGPLMAGMNVVGDLFGSGKMFLPQVVKSARVMKQAVAYLTPYLEE
                     EKRRTGADQRSAAGKVLMATVKGDVHDIGKNIVGVVLQCNNYEVIDLGVMVPCQKILE
                     TARKEKVDIIGLSGLITPSLDEMVFVASEMEREGFSLPLLIGGATTSRVHTAVKIAPA
                     YDKGQAVYVTDASRAVGVVSSLLSPEQNDNYTAGLRAEYAKVAAAHARAEAEKSRLPI
                     ERARENRFRPDWATYTPPKPSFTGTRIFNTYDLGELVPYIDWTPFFQTWELKGRFPAI
                     LEDAEQGPAARQLYADAQAMLKRIVDERWFQAKAVIGFWPANAVGDDIRLYTGESRAE
                     TLATFYSLRQQLTRRDGKPNLALADFIAPEGCGKADYLGGFVVSAGFGEDRIAERLKG
                     KNDDYSSILAKALADRLAEAFAERMHERVRKEFWGYAEDERHAPQSLITETYRGIRPA
                     PGYPAQPDHTEKATLFALLAAERRIGVKLTESFAMWPGASVSGLYFAHPDAYYFGVAK
                     VERDQVEDYAARKGMAIAEVERWLAPILNYDPAGYAIEAA"
BASE COUNT         1115 a         2482 c         2350 g         1376 t
ORIGIN      
        1 gggtgcggtt gccggcgcgg ccattggcgg catcggcggc gcggtcatcg gtggcaccac
       61 ggcgccgcgc cagcgcgaat gcgctcgctg gggccgcgac tattacggca acgtcgtctg
      121 caccgcctac tactgagcgg caacgccttc cgccttgccc tggccgcgtc ggctgatcga
      181 taagtcagtc gatgcggcca cagtgtgatt ctccccttgc acggctgttt gcctcgccga
      241 accacggcga gcgcaaggac ggcttgcgac cggatatgat catcctccat tacaccggca
      301 tgccgacggg cgatgcggcc ctcgcctggc tgtgcaatcc ggtatcagag gtttccagcc
      361 actatttcgt gcgcgagaat ggccgaatcc tgcaactggt gccggaggcg cggcgcgcct
      421 ggcacgccgg cgcctcatcc tggcagggcc tcggggatat caactcccgc tcgatcggca
      481 tcgagatcgt caacgccggg cacgaaggcc acggcatcga tcctgtcgat ccccgacggc
      541 cgccggatgt gacggcgccg gacaaaggcg ccggcccatt gcccgcgttc ccgcctgcac
      601 agatcgaatc tgtgatccgc ctggtgcgcg acatcgccgc gcgctggcac atccggcccg
      661 aacgcattct cgcccattcc gacatcgccc cgaggcgcaa gcgcgacccc ggcgaggcct
      721 tcccctggcc ggttctggcg gaaaacgggg tcggccattg ggtgaggcca acgcccattc
      781 gctccggccg gtttttccag cccggcgacg gcggcgcgcc ggtggaagcc ttgcagcaga
      841 tgctgagcct ctatggctac gacatgcccg cgaccggccg ctatgacgag cagaccgagg
      901 cggtcgtgcg cgccttccag aggcattttc gcccggaacg ggtggacggc atcgccgatt
      961 tctccaccat aaccacgctg cgcgatctca tccgcgcttt gccggacggg cgctgatgtt
     1021 cgtctatccc gaggatcgca agcgcctgac cggactggcg atgcaattgc tggcgctggc
     1081 cgagcagcgg tttggtccgc gcgttccagg ctggaccttt ctcggcgtct tcttcgattc
     1141 cgatggcccc tctatcgttt tccccacgcg ggcgcgcaac gagctggaga tgcgcgtgac
     1201 cttctcctgc cgcaacaacg ccgatctcgc cggcttccag atcggccacg aggtgatcca
     1261 catgcttgcg ccggatcgca atccgcccgc cagcatgctg gaggaaggca tcgccacgct
     1321 gtttagcctg gaaggccctt cgttcctctc gccgacctac cgcacctggt cgatccacta
     1381 cctggcgacc caccctcagg tgcatcacta caacgaggcg ctggggctgg tgcgcgaact
     1441 gctggcgatc cagccggacg cggtgatggc gctccggcgg cgcgagccgc gcatccatgc
     1501 ctttaccgcc gaattcatca cgcaaaccct gccccgcgtg ccgaaatcgc tcgctgcgcg
     1561 gctgatggat cggcgcaaga tgcggtgagc cccgcgccct gcgccggact tacacgcttg
     1621 ctttacgcct gccccctagg gtccctcccg tagggagaat cttcgatgac atcgctggac
     1681 gttacccagg ctcgcggcaa acatgcagga atgacgcagg cgcgtctcgt cctgatagtt
     1741 tacgcggtgg cgatcttcgc gtccgcgttc ctgctgtttt cggtgcagcc gatgttcacg
     1801 cgcatggtgc ttcccaagct cgggggaacg ccggcggtct ggtcggtggc catggtcttc
     1861 ttccagaccg tgctgttgat cggctatctc tatgcgcatc tgtcggtgac ctggctgtcc
     1921 gcccgcgccg ccagcatcgt ccacctctcc atcatgggcg ttgccgcact gggcctgccc
     1981 atcgcggtca gcgaacttct gggcgatccg ccgcagacag gggctgaatt ctggctcttc
     2041 gcggtcttcg ctctgtcggc cggactgccg ttcttcgcca ttgccggcaa tgggccgtta
     2101 ctgcagcagt ggtatcatcg ggccgctggc ccgagcgcaa gcaatcccta caccctgtat
     2161 ggcgcctcga acctcggcag cttcggcgcg ctcctgctgt accctttcgc gttcgaaccg
     2221 ctgttcgccc tcaagacgca gagccatctc tggtcgatcg gtttcgcgat gctcgccgtg
     2281 gtcatcgccc tgtgcggggt tctgctggtg cgggcggccc ccggcagggt cacgacaaac
     2341 cagaaggacg ccgccgccac ggcagagacg ccttcctccg ggcggaagct ggcatgggtc
     2401 ttctatgcct tcgtgccttc cggctatctc gtcgcggtaa ccgcgcacat ctccaccgac
     2461 atcgcctccg caccgctgct gtggatcatt ccgcttgcac tgttcctgct gacgttcgtt
     2521 gccgtgttca gggacacgcc gctgctgccg ccggcgctgt tcacgcgggc ctggccgatc
     2581 gccttcctct tgctgttgct gccggactgg atcgagatgc cgatcgcggt gagcatcgcg
     2641 ctccatgtcg ttggcttctt cgtcgccacg atggtctgcc accaggcgct gtatgcgtcg
     2701 cgtccggccg ccagtcatct cacgctgttt tacctgctga tgtcgcttgg cggcgtcctg
     2761 ggcggtgtct tcgccagcct cgtgagcccg catgtgtttt ccggcgtgca cgaatatctg
     2821 atcctgatgg ttctggtgtt cctgtgcttg cccggcgcca tgaacgccgt gcgcgcccat
     2881 ctcgcggcga ttctgctcgc cctggcgggc gcggccgtgc tcatcgtcgg catgcgctcg
     2941 tccctgccgg tcacgctgga cccgaccgcc gtggcgctgc cggtgctcgt ggccagcgtc
     3001 gccttgttct ggacggaacg gcatgcgccg ctgcgcctgg ccgcgatcgg cggcgcctgc
     3061 ctcgcggtcc ttgcctttgc caaggatgac ggcaaggtcg agcatgtcac gcgttcgttc
     3121 ttcggcgtca acaaggtgat gatgatcgag ggtggcaagt accgcgccat cgtccacggc
     3181 aacaccaacc atggcgcgat gcgggtgctg aacgatgacg gcacgcccgc gacgggccgg
     3241 caacgccccg tcgcctatta tcgcgacggc gcgccctacg agaccgcgat ttcccggatc
     3301 agacagcgac agggcgggct tggctcggtc gcgatcaccg gcctcggcgc cggcggcctc
     3361 gcctgtcacg cccgcgacgg cgagaagtgg acgttcttcg aaatcgatcc ggaagtcatc
     3421 cgcatcgctc aggacacccg ctggttccgg tccctgtcgg actgcacgcc ggatgcgcgg
     3481 atcatcgccg gcgatgccag actgacgctg gccaagcacc ccggcggctt cgatctcatc
     3541 gccctcgacg ccttttcgtc ggatgccatt ccggtgcatc tgctcaccga ggaagccatt
     3601 cagctctact tctccaagct caatgcggac ggcgcgctga tcattcatat caccaaccgc
     3661 tacatggagc tgcgcagcgt tgtcgccgcc atcagcgcca ggctgaatct ggtcggcgtc
     3721 gcgcgcaagc tgacgcagga ggaggcgatg gccgagagca tgtcggacat gccggtcggc
     3781 gtcgaggtcg ccgtgctggc ccgcaagaag gaagacctcg gcgccctgcc cggccttccg
     3841 ggctgggaca tgctgaaggc cgacccctcg gtcgcgtcct ggtccgacga ctactcgaac
     3901 attgtcggcg cgttgctgcg caaatagcgc acgccgtcag gccgcctcga tcgcatagcc
     3961 ggccggatcg tagttgagga tcggcgcgag ccagcgctcg acctctgcga tcgccatgcc
     4021 cttgcgggcg gcatagtcct caacctggtc gcgctcgacc ttggcgacgc cgaagtaata
     4081 ggcgtcggga tgggcgaagt agaggcccga gacggaagcg ccgggccaca tcgcgaagct
     4141 ttcggtcagc ttcacgccga tccgccgctc ggccgcgaga agcgcgaaca gtgtcgcctt
     4201 ttcggtgtga tcgggttggg cggggtagcc cggcgcggga cggatcccgc gatacgtctc
     4261 ggtgatcagg ctttgggggg cgtgccgttc gtcctcggca tagccccaga actccttgcg
     4321 cacgcgttcg tgcatgcgtt cggcgaaggc ttccgccagc cggtcggcca gtgccttcgc
     4381 gagaatcgag gaataatcgt cgttcttccc cttcagccgt tcggcgatac ggtcctcgcc
     4441 gaagccagcg gaaaccacga agcccccgag gtaatccgcc tttccgcacc cctccggcgc
     4501 gatgaaatcg gcaagcgcca ggttcggctt gccatcgcgg cgcgtcagct gctggcgcag
     4561 gctgtagaaa gtggccagcg tttcggcgcg gctctcgccc gtgtagaggc ggatgtcgtc
     4621 gcccaccgcg ttcgccggcc agaagccgat caccgccttg gcctggaacc agcgctcgtc
     4681 gacgatgcgc ttgagcatcg cctgcgcgtc cgcgtagagc tggcgcgccg caggcccctg
     4741 ctcggcatcc tccagaatgg ccgggaaacg gcccttcagt tcccaggtct ggaagaacgg
     4801 cgtccagtcg atatacggga ccagttcgcc gagatcgtag gtgttgaaaa tgcgcgtgcc
     4861 ggtgaagctg ggcttcggcg gcgtgtaagt ggcccagtcc ggccggaacc ggttctcccg
     4921 cgcgcgctcg atcggcagac gcgacttttc cgcctctgcg cgggcatggg ccgcggcgac
     4981 cttggcgtat tctgctctca ggccggcggt gtaattgtcg ttctgttcgg gcgagagcag
     5041 cgaggacacg acgcccacgg cgcggctggc gtccgtcacg tataccgcct gacccttgtc
     5101 gtaggccgga gcgatcttga cggctgtatg cacgcggctc gtggtggcgc cgccgatcag
     5161 cagcggcagg gaaaagccct cgcgctccat ctcggatgcg acgaacacca tctcgtcgag
     5221 cgagggcgtg atcagaccgg acaggccgat gatgtcgacc ttctccttgc gcgccgtttc
     5281 caggattttc tggcacggca ccatcacgcc gagatcgatc acctcgtagt tgttgcactg
     5341 gagcacgacg ccgacgatgt tcttgccgat gtcgtgcacg tcgcccttca cggtggccat
     5401 cagcaccttg ccggcagcag aacgctggtc ggccccggtg cggcgctttt cttcctcaag
     5461 atagggcgtg agataggcca ccgcctgctt catgacgcgg gctgacttca ccacctgcgg
     5521 caggaacatc ttgccggagc cgaaaaggtc gccgaccacg ttcatgccgg ccatcagcgg
     5581 cccttcgatg acgtgcagcg ggcgttcggc tgcggcgcgc gcctcctcga cgtcgatctc
     5641 gatgaactcg gtgacgccgt tgaccagcgc atgctccagc cgcttgtcca ccggcagcct
     5701 gcgccactcc aggtcggcgg tcttcacctc ggccttgccg tcgcccttga aacgcggcgc
     5761 ggcatccagc aggcgctcgg tggcatccgg gcggcggttg agcacgacgt cctcgcacag
     5821 gtcccgcaac tcctgtggca gcgcatcata gaccgcgagc tggccggcgt tgacgatgcc
     5881 catgtccatg ccgacgcgga tgcaatggta gaggaacacc gcgtgcatcg cctcgcgcac
     5941 cggctcgttg ccgcggaagg agaacgacag gttggaaacg ccgcccgaga catggcagtg
     6001 gggcaggctc tggcggatcg tcctggtcgc ctcgatgaag gcgacgccgt agccgttgtg
     6061 ctcctcgatg cctgtggcca cggcgaagat gttcgggtcg aagatgatgt cttccggcgg
     6121 gaaaccgacc ttctccgtca gaaccttgta ggcccgcgtg cagatctcga tcttgcgctc
     6181 ataactgtcg gcctggccct gctcgtcgaa ggccatcacc acgaccgccg cgccataggc
     6241 ccggcacagc ttcgcgtggt ggatgaaggc ctcctcgccc tccttcatgg agatcgaatt
     6301 gacgatgggc ttgccctgca cgcatttcag cccggcctcg atgacgttcc acttggaact
     6361 gtcgatcatc agcggcaccc gcgcgatgtc cggttcggcg gcgatcaggt tgacgaaggt
     6421 cgtcatcgcg gcttcggaat cgagcagacc ctcatccatg ttgatgtcga tgacctgcgc
     6481 gccgttggcg acctggtcgc gcgccacgtc gagcgcctcg gggtatttgc cttccttgat
     6541 cagcttgcgg aagcgggccg agccggtgac gttggtgcgc tccccgatgt tgacgaaggg
     6601 aatgtccttg gtcagcgtga aaggctccag accggagagg cgcagcagcg gctctgcctt
     6661 gggcacgcgc ctcggcgtct tgccggccat cgcatcggcg accgcccgga tatgcgccgg
     6721 cgtcgaaccg cagcagccgc cgacgatgtt cacgaggccg tccgaggcga agtcgccgat
     6781 catgcgcgcc atcgcctccg ggctctcgtc gtaaaggccg aattcattgg gcaggccggc
     6841 attgggatag gcgcagacca gcgtatccgc gaccttggaa atctctgcga tatgagcacg
     6901 catttcccgc gcgcccagcg cgcagttcag gccgatcgac agcggatcgg catggcgcag
     6961 cgaataccag aaagcggtcg gtgtctgacc ggaaagcgtt cggccggaca ggtcggtgat
     7021 ggtgccggag atcatcagcg gcagccgcac gccggtcgcg acgaaggcct cctcggcggc
     7081 gaagacagcg gccttggcgt tgagcgtgtc gaagatggtc tcgatcagga tcagttccgc
     7141 gccgccctcg atgaggccac ggatctgctc gccataggcg atcctgaggt cgtcgaaggt
     7201 caccgcgcgg tagccgggat tgttcacgtc cggcgagatc gaggcagtgc ggttggtcgg
     7261 tccgactgcg ccggcgacga agcgccgacg tccgtccttc gcctgcgcca gcgcggcggc
     7321 ctc
//
LOCUS       DAOMXN010000128         7290 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_5334_length_7290_cov_9.388943, whole genome shotgun sequence.
ACCESSION   DAOMXN010000128 DAOMXN010000000
VERSION     DAOMXN010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 7290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7290
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5334_length_7290_cov_9.388943"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="PK812_09380"
     CDS             <1..577
                     /locus_tag="PK812_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69809.1"
                     /translation="SGVRPRPAAPDAAPLPVSSRILFFEETPSDPRGIQSDGMIIWRL
                     DTVSTPGQPADRVVRGAMSIPNAKMGGEIVLKRNRNAALPASHTMEIIFRPEGDREGV
                     REISPIEARDQETNPGVALQGAMVPVATNLFLIGLDRSESGMARNLESLRDKRWLAFQ
                     VRFVSGKIGAVLVEKGQTGDRVFREALESWR"
     gene            complement(672..1559)
                     /gene="rpoH"
                     /locus_tag="PK812_09385"
     CDS             complement(672..1559)
                     /gene="rpoH"
                     /locus_tag="PK812_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HRJ69810.1"
                     /translation="MTTAAIPVVAGERGLARYLQEIRRFPVLKPDQEYMLAKRFREHE
                     DPQAAHTLVTSHLRLVAKIAMGYRGYGLPVAEVISEGNIGLMQAVKRFEPDRGFRLAT
                     YAMWWIRASIQEYILRSWSLVKIGTTANQKKLFFNLRKAKSRISALGEGDLRPDQVRE
                     IATKLGVEEQDVIDMNRRLGGDASLNAPLREDGAGQWQDVLPDESADQEAVLMAGEEG
                     DNRLKALRSAVATLNDRERRIFEARRLADDPMTLEDLSTEFNVSRERVRQIEARAFEK
                     VQAAVRRNLAAIEAGQAAA"
     gene            complement(1771..2280)
                     /gene="msrA"
                     /locus_tag="PK812_09390"
     CDS             complement(1771..2280)
                     /gene="msrA"
                     /locus_tag="PK812_09390"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015916426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HRJ69811.1"
                     /translation="MSTETAILAGGCFWGMQDLIRRRPGVISTRVGYSGGDVPNATYR
                     NHGTHAEAIEVIFDPAVTSYRDLLEFFFQIHDPTTVNRQGNDLGMSYRSGIYYTSAAQ
                     KAEAERTIADVNASGLWPGKVVTEVKPAGPFWQAEPEHQDYLERIPNGYTCHFPRPNW
                     KLPRRAGAA"
     gene            complement(2334..3335)
                     /locus_tag="PK812_09395"
     CDS             complement(2334..3335)
                     /locus_tag="PK812_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRJ69812.1"
                     /translation="MAPETLVEPKILAPEQAGERLDRWLALTFPEHSRSRLKALILDG
                     AVTVNGRSVTDPALKLKVGDAVVLALPPPAAAEPRPEAIPLKVVHEDEDLIVIDKPAG
                     LVVHPSAGHETGTLVNALLHHCGASLSGINGVLRPGIVHRLDKDTSGLLVVAKNDAAH
                     KKLADQFADHGRTGALERLYLALCWGVPMRPHGTIDAAIARSTVNREKMAIVAGDKGR
                     FAVTHYEVRETFAGADTKPVAALIDCALETGRTHQIRLHMAHVGHPLLGDEVYGAGFR
                     TKASLLPPKARAALEKLGRQALHAAVLGFEHPRSGKVLHFESPPPPDLARLIKTLRG"
     gene            3450..3525
                     /locus_tag="PK812_09400"
     tRNA            3450..3525
                     /locus_tag="PK812_09400"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3484..3486,aa:Glu,seq:ctc)
     gene            complement(3566..5329)
                     /locus_tag="PK812_09405"
     CDS             complement(3566..5329)
                     /locus_tag="PK812_09405"
                     /inference="COORDINATES: protein motif:HMM:NF015083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HRJ69813.1"
                     /translation="MQNASYSLNRRGLIQFGIAVGAQALAGPMILNRARSSTETLAPN
                     APGFRRLFPDLPAARHDGDDLMRLALGDGRNLSGMSAPPEVRKNHKGEPARDAEGRLI
                     IAATAEDKQDDEENFGLPAGYTYLGQFVDHDITFSPGDSLDPAHQPSAANLRSARFDL
                     DCLYGRGPADQPYLYEADGRRLVSGRRLAASTRNGVPADHPRAQGRALIGDKRNDENV
                     LVSQLHGVFRAFHNQIAHDDPKADFSALQSQVTRHYQWVLLTDFLPRICGRETIESIL
                     PGFGHGGRTGYYRPHLKLTKPLRFGEIPLEFSGAAYRFGHSLIRPAYRINLHMTGTAA
                     ERRDNPELAGRRLIFAAAQYGGLNGFRPIPEEWGIDWRLYFEIDRKLDPARIGDGRRR
                     VQAAYKIDTSLTNPLAFLPEFSEAGKGGDLAADPNGQPRPRPGAVSNLAARNLLRGFQ
                     IGLPSGQEVARAMGIDPIADRDLLVGKATVDGLSENRSITEYGASFRDSAPLWFYILA
                     ESQHLWAQKARAHSGTKEQRDAIPTVLGPVGAQIVAETFVALMQLDPRSILHAGPDWS
                     PRYLRNNRFSMAELVLGAHLA"
     gene            5477..6328
                     /locus_tag="PK812_09410"
     CDS             5477..6328
                     /locus_tag="PK812_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6502 family protein"
                     /protein_id="HRJ69814.1"
                     /translation="MSDQPSSGRSGPSASELQPALARLLRPLVRLMIRSGITFPALSD
                     TLREVYVSVAEKEFALRAKPQTDSRVSLLTGVHRKEVRRLREAGVPVSETPAMVSRSS
                     LILAHWLGSPDFTDTAGEPRPLHRFSDGPDGPGFDSLVAAVTRDVRPRAVLDELLEQE
                     IVSLDDEGRVHLLATALVPSRDMDALSYYFGRNLHDHLAAAVENLAGRTPAFLERAVH
                     YEGLSEATTVALEAKSRELALAALQAANREALAARAEEPSGEGSVRDWRWTFGLYIFR
                     ERTQPKP"
     gene            6325..>7290
                     /locus_tag="PK812_09415"
     CDS             6325..>7290
                     /locus_tag="PK812_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69815.1"
                     /translation="MTRHSPSRRDMLLAALTGLFAAIPARAQQSQGPGDNGIGGTGLP
                     RRAGDGIGGTGVHGAISGFGSIIVNGARISYAPDAVVHADGRRLRASALRIGHVVRVG
                     ASGSGAALAARWIELDRIVIGPVTAVGWLSREIRVLGQRVIPPSGGERPGVGTWIAVS
                     GFRRADGVIEASLIEPTEERVARVTGTLDRLADGAGLGIGGLTVDAPARRAGQRVRVS
                     GDVENGRLVAPRIASAPGAPEGRRILVQGFATRSGDRMVFADGGLAVRLPGGVAWASS
                     DAKVVVHAERGADHSWIAVTVSDSGGLGGEIGSSAARAPAGRGGAA"
BASE COUNT         1108 a         2323 c         2589 g         1270 t
ORIGIN      
        1 atcgggcgtg cggccgcggc cggcggcgcc cgatgccgcg cccctgccgg tttccagccg
       61 tatcctgttc ttcgaggaaa ccccatccga tccgcgcggc atccagagcg atggcatgat
      121 catctggcgg ctcgataccg tcagcacgcc gggccagcct gccgaccgcg tggtccgcgg
      181 cgcgatgagc attccgaacg ccaagatggg cggagaaatc gttctcaagc gcaatcgcaa
      241 cgccgccttg ccggcatccc acacgatgga gatcatcttc cgtccggaag gcgaccgcga
      301 gggtgtccgc gagatcagtc cgatcgaggc gcgcgaccag gagaccaatc ccggcgtcgc
      361 gctgcagggc gccatggtgc cggtcgccac caacctgttc ctgatcgggc tcgaccgttc
      421 cgaatccggc atggcgcgca acctcgagtc gctgcgcgac aagcggtggc tggcgttcca
      481 ggtccgcttt gtgtccggca agatcggcgc cgtgctggtc gaaaagggcc agaccggcga
      541 ccgcgtgttc cgcgaagcgc tggaatcctg gcgctagggc actatccgac cggaatggtt
      601 caatgtgatc ggatagtgct tcactaccag ggggcacgtg ccgtgacccc tggtttgaaa
      661 gccgcgtcgc gtcaggccgc tgcctgtcct gcctcgatgg ccgcgagatt gcggcgcacg
      721 gcagcctgca ccttctcgaa ggcgcgggct tcgatctgac gcacgcgctc gcgcgagacg
      781 ttgaactcgg tcgacaggtc ctcgagcgtc atcgggtcgt cggcaaggcg acgggcctcg
      841 aagatgcggc gctcgcgatc attcagggtg gcgacggccg agcgcagcgc cttgaggcgg
      901 ttgtctccct cctcgccggc catcagcacg gcttcctgat cggcgctctc gtccggcagc
      961 acgtcctgcc actggccagc accgtcctcg cgcagcggag cgttcagcga cgcgtcgccg
     1021 ccgaggcggc ggttcatgtc gatgacgtcc tgctcctcga cgccgagctt cgtggcgatc
     1081 tccctcacct ggtccgggcg caggtcgccc tcgccgagcg cggaaatgcg gctcttggcc
     1141 ttgcgcaggt tgaagaacag cttcttctgg ttggccgtgg tgccgatctt caccagcgac
     1201 cacgaacgca ggatgtattc ctggatcgag gcgcggatcc accacatggc gtaggtggcg
     1261 aggcggaagc cacggtccgg ctcgaagcgc ttcacggcct gcatcaggcc gatgttgccc
     1321 tccgagatga cctcggcgac cggcagcccg taaccgcgat agcccatggc gatcttggcg
     1381 acgaggcgca gatgggatgt aacaagggtg tgcgcggcct ggggatcctc atgctcgcga
     1441 aaccgcttcg ccagcatgta ctcctggtcc ggcttgagaa ccggaaagcg ccggatctcc
     1501 tggagatacc gggcaagacc cctttcgccc gcgacaacgg ggatagcggc agtcgtcatt
     1561 tcgtcctcct tcgggccccc catccgggca gaccctccac cggagcgata cgcctccggc
     1621 tgcgcgagcc ttataccaac atccggctcg aaaagaaaga ggttcttgtc ctgttcccct
     1681 ttctcgccgt tgcgcttcta gaggcagggg cctgctcggg atgtgcgctg gcgcacatcg
     1741 ccggggttga cggggccgtc agcagaggcg tcacgccgcc ccggcgcgcc gcggcagctt
     1801 ccagttcggg cggggaaaat ggcaggtgta gccgttgggg atgcgctcca ggtagtcctg
     1861 atgctccggt tcggcctgcc agaacgggcc ggccggcttc acctcggtca ccactttgcc
     1921 gggccacagg ccggaggcgt tgacatcggc gatggtgcgc tcggcctccg ccttttgcgc
     1981 ggcactcgtg tagtagatgc cggaacggta ggacatgccg agatcgttgc cctggcggtt
     2041 caccgtggtc gggtcgtgga tctggaagaa gaattcgagc aggtcgcggt agctcgtcac
     2101 ggccggatcg aagatcacct cgatcgcctc ggcatgggtg ccgtggttgc gataggtagc
     2161 gttcggcacg tcgccgccgc tgtagccgac gcgggtggag atcacgccgg ggcgcctgcg
     2221 gatcaggtcc tgcatgcccc agaagcatcc gcccgcgaga atggccgttt cggtgctcat
     2281 gatcccctcc gtctgcgcgg ccttgccgcg ccggtcaata tggggcgact gcctcagccc
     2341 cgcaacgtct tgatcagccg cgcgaggtcg ggcgggggcg ggctctcgaa atgcaggacc
     2401 ttgccgctgc gcgggtgctc gaagccgagg acagcggcgt gcagcgcttg ccggccaagc
     2461 ttctccagcg cggcgcgggc cttcggcggc agcaggctcg ccttggtgcg gaagcccgcg
     2521 ccatagactt catcgcccag gagcgggtgg ccgacatggg ccatgtgcag gcggatctgg
     2581 tgggtgcggc cggtttccag cgcgcagtcg atcagcgcgg ccacaggctt tgtgtcggct
     2641 cccgcgaacg tctcccgcac ctcgtaatgg gtgacggcga agcggccctt gtccccggcg
     2701 acgatggcca ttttctcgcg gttgacggtc gagcgggcga tggcggcgtc gatggtgcca
     2761 tgcgggcgca tcggcacgcc ccagcagagc gcgagataca gccgctccag cgcgccggtg
     2821 cggccatggt cggcgaactg gtcagcgagc ttcttgtgcg cggcgtcgtt cttggcgacg
     2881 acgagcaggc ccgaggtgtc cttgtcgagg cggtggacaa tgccagggcg cagcacgccg
     2941 ttgatgccgg acaggctcgc gccgcaatgg tgcagcaggg cattgacgag cgtgccggtc
     3001 tcatgcccgg cgctggggtg caccaccagt ccggccggct tgtcgatgac gatgaggtcc
     3061 tcgtcctcgt gcacgacctt gagcgggatc gcttccggcc gcggttccgc ggcggcgggt
     3121 ggcggcaggg ccagcacgac cgcatcgccg accttcagct tcagcgccgg gtcggtgacg
     3181 gagcgtccgt tgacggtgac ggcgccgtca aggatgagcg ccttgagccg gctgcgcgaa
     3241 tgttcgggga aggtcagagc aagccagcga tccagccgtt cgcccgcctg ttcgggggcg
     3301 aggatcttgg gttctacgag ggtttcgggc gccatccgcc gcatgtaggc ccttggcgca
     3361 aggggtgcaa tggcggtgtc gaaaacgcct tgccgccccg ggcgcgcggc gctatagggg
     3421 cgcgtgtgca gcgccccctg gggcgttccg ctcccttcgt ctagcggtct aggacgcggc
     3481 cctctcaagg ctgaaacacg ggttcgattc ccgtagggag cgccaaaaaa acaagcaatt
     3541 tcaaagattt gttcgacgat tcccgctatg ccagatgggc gccgagcacc agttccgcca
     3601 tgctgaaacg gttgttgcgc aggtagcggg gcgaccagtc cgggccggca tgcaggatcg
     3661 agcggggatc gagctgcatc agcgcgacga aagtctcggc gacgatctgc gcgccaaccg
     3721 ggccgagaac ggtcggaatg gcgtcgcgct gctccttggt cccggaatgg gcgcgggcct
     3781 tctgcgccca taaatgctgc gattccgcga ggatatagaa ccataatggc gcggaatcgc
     3841 gaaaagaggc cccatactcg gtgatggagc gattttccga aaggccgtcg accgtcgcct
     3901 ttccgaccag caagtcccgg tcggcgatcg ggtcgatgcc catggcgcgg gccacctcct
     3961 gcccgctcgg caggccaatc tggaagccgc gcaacaggtt tcgggcggcg aggttggaga
     4021 ccgcgccggg cctgggtctg ggctgaccgt tcgggtcggc ggcgagatcg ccgccttttc
     4081 cggcttccga gaattccggc aggaatgcca gtgggttagt cagcgatgtg tcgattttgt
     4141 aggcggcctg cacgcgccgc cggccgtccc cgatgcgcgc cgggtcgagc ttgcggtcga
     4201 tctcgaagta gagccgccag tcgatgcccc attcctccgg gatggggcga aagccgttga
     4261 ggccgccgta ctgcgcggcg gcgaaaatca gcctgcggcc ggcgagctcc gggttgtcgc
     4321 gccgctccgc cgccgttccc gtcatgtgca ggttgatccg gtaggccggg cggatcagcg
     4381 aatggccgaa ccggtaggcg gcgccggaga attcgagcgg aatttcgccg aatcgcaggg
     4441 gtttcgtcag cttcagatgc ggccggtagt agccggtgcg accgccgtgg ccgaagcccg
     4501 gcaggatgga ctctatcgtt tcccggccgc agatgcgcgg caggaagtcg gtgagcaaga
     4561 cccactggta atgccgggtg acctgcgact gcagcgctga gaaatccgcc ttcgggtcat
     4621 catgggcgat ctggttgtga aaggcgcgga aaacaccgtg caactggctg accagcacgt
     4681 tttcgtcgtt gcgcttgtcg ccgatcaggg ccctgccctg ggcgcgcgga tgatcggcgg
     4741 gcacgccgtt gcgcgtgctg gcggcgaggc gacggccgct gaccaaccgc cggccgtccg
     4801 cctcgtaaag atagggctgg tcggcgggcc cgcgcccgta gagacagtca aggtcgaacc
     4861 gcgcgctgcg caggttcgct gccgagggtt gatgggcggg atccagcgag tcgccggggc
     4921 tgaaggtgat gtcgtggtcg acaaactggc cgagataggt gtagcccgca ggcaggccga
     4981 agttctcttc gtcgtcctgc ttgtcttccg ccgtcgctgc gatgatgagc cgaccctcgg
     5041 cgtctcgcgc cggctcgccc ttatggttct tccggacttc cggcggggca gacatgcccg
     5101 acaggttgcg gccatccccg agggcgaggc gcatcagatc gtcgccatca tgccttgccg
     5161 ccggcaggtc ggggaaaagc cgccggaagc cgggagcgtt cggcgcgaga gtctccgttg
     5221 aactgcgcgc cctgttcagg atcatcggcc cggcgagagc ctgcgcgccg acggcgatgc
     5281 cgaactgaat gaggccgcgc cggttgaggc tgtaagatgc gttctgcatg ggacgttccc
     5341 tgccgcgagt ggtgttgccg atccgcaccg ccacttccag agcgcggcac ctgcggcgaa
     5401 tggtgcgtgg gaaaatttta cacgtcaaga aaaatgtggg attgtttccc acgaaattgt
     5461 cggctaccgt gtcggcatgt ctgatcagcc gtcctccggt cgcagcggtc ccagcgccag
     5521 tgagttgcaa ccggcgcttg cgcggctgct gcgaccactg gtgcgcctga tgatccgttc
     5581 gggcatcacc tttccggccc tttcggacac gctgcgggag gtctatgtca gtgtcgcgga
     5641 aaaggaattt gcgctgcggg caaagccgca gaccgactcc cgcgtatccc tgctgacggg
     5701 tgtgcatcgc aaggaggtgc gccgcctgcg tgaggccggc gtgccggtca gtgagacgcc
     5761 ggccatggtt tcgcgcagca gtctcatcct ggcgcattgg ctcggatcgc ccgacttcac
     5821 cgacacggcc ggcgaaccgc ggccgttgca ccggttctcc gacggtcccg atgggcccgg
     5881 tttcgacagt cttgtcgccg cagtgacgcg ggacgtgcgg ccacgcgcgg tgttggacga
     5941 gctcctggag caggagattg tctcgcttga cgatgagggc cgggtgcacc tgctcgccac
     6001 ggcgctggtt ccatcgcgcg acatggacgc gctgtcgtat tattttggcc gcaatctgca
     6061 tgatcacctg gcggcggcgg tcgagaatct ggcgggacgg accccggctt tcctggagcg
     6121 tgccgttcac tacgaagggt tgagcgaagc gacaaccgtg gcgctggagg caaagtcgcg
     6181 tgagcttgca cttgccgcgc tccaggctgc gaatcgagag gcgcttgccg cccgggctga
     6241 agaaccatcg ggcgagggct cagtcaggga ctggcgttgg acgtttggcc tttacatctt
     6301 tcgggaaagg acccagccga aaccatgacc cggcatagcc cctcgcgccg cgacatgctg
     6361 ctggccgccc tgaccggcct gtttgccgcc ataccggcac gcgcccagca gtcccagggc
     6421 cccggcgaca acggaatcgg tggaaccggg ttgccccggc gcgccggcga cggcattggg
     6481 ggcaccggcg tgcacggcgc catttccggg ttcggcagca tcatcgtcaa tggcgcgcgc
     6541 atcagctacg ccccggatgc cgtggtgcat gccgacggcc gcaggcttcg ggccagcgca
     6601 ctgcgcatcg gacatgtggt tcgggtcggt gccagtggat cgggcgcagc gcttgcggcg
     6661 cggtggatcg agctcgaccg catcgtgatc gggccggtca cggcggtggg ctggctgtcg
     6721 cgcgagatca gggttctggg tcagcgcgtg atcccgccat ccggcggcga gcggccgggc
     6781 gtcggtacgt ggattgcggt gagcgggttc cggcgcgcgg acggcgtgat cgaagcgagc
     6841 ctgatcgagc cgaccgagga acgcgtcgcc cgggtcacgg gcacgttgga tcgacttgca
     6901 gatggggccg gcctcggtat tggcggattg accgtggatg cgccggcgcg acgggccggg
     6961 cagcgtgtcc gcgtttccgg tgacgtcgag aacgggcgcc tggttgcgcc acgcatcgcc
     7021 agtgcgccgg gtgccccgga agggcgccgg attctcgtcc agggatttgc cacacgttcg
     7081 ggcgaccgga tggtcttcgc cgatggcggg ctggcggttc gactgcccgg cggcgtcgcg
     7141 tgggcctcgt cggacgccaa ggtcgtggtg catgccgaac gcggcgccga tcacagctgg
     7201 attgcggtga ccgtgtccga ttcgggcggg ctgggcggcg agatcggatc ttccgccgcc
     7261 cgtgcgccgg cgggacgtgg cggagcggcc
//
LOCUS       DAOMXN010000129         7271 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_5356_length_7271_cov_11.283259, whole genome shotgun sequence.
ACCESSION   DAOMXN010000129 DAOMXN010000000
VERSION     DAOMXN010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 7271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7271
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5356_length_7271_cov_11.283259"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PK812_09420"
     CDS             <1..234
                     /locus_tag="PK812_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011923459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HRJ69816.1"
                     /translation="HGALVAFIHRAQASGASLVVIVTGKGAPGLDSERGVLRRLVPHW
                     LADPALRRCIVGFEPAGRGHGGDGALYVRIRRP"
     gene            291..1286
                     /locus_tag="PK812_09425"
     CDS             291..1286
                     /locus_tag="PK812_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HRJ69817.1"
                     /translation="MTAQFDILGIGNAIVDVIAHSDEAFLERFKVAKGSMTLIDAELA
                     ETLYAAMGPATIMSGGSGANTAAGAASFGARVAYVGKIKADAVGDIFAHDIGALGVSY
                     TTRRAESGPPTARCFVLVTPDGERTMNTYLGACQNLSERDIDPEVVGGAKVTYLEGYL
                     WDPPEAKKAFRKAADIAHKAGREVSLTLSDSFCVDRYRDEFRDLVRTGMVDLLFANES
                     ELKALYQTADFDTALAALRQEANKAVVTRSEKGAMFVSRAETVAVPAYPIEQLVDTTG
                     AGDLFAAGFLHGYTAGRSSADSLRLGALAASHIIQQLGARPQVELKQLARDNGLG"
     gene            complement(1296..2264)
                     /gene="coaA"
                     /locus_tag="PK812_09430"
     CDS             complement(1296..2264)
                     /gene="coaA"
                     /locus_tag="PK812_09430"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HRJ69818.1"
                     /translation="MDAKASQLGSAVDLSPYRVFSREEWANLRADTPLTLTAADLKKL
                     QSLNDPISLDEVIEIYLPLSRLLSLYVAATQGLFKATQRFLLAEDGKVPYIIAVAGSV
                     SAGKSTTARVLKALLSRWPNTPKVELVTTDGFLLPNAELQRQGLMERKGFPESYDGAA
                     LIKFLAQVKAGQRNVEAPVYSHLVYDIVPDEKIVVDRPDILIFEGINVLQGPRPAKDG
                     RAIPFVSDFFDFTVYVDADEEDLRRWYVARFMRLRETAFRDPKSFFRKYAELAEDEAL
                     RTANGIWERINLKNLWENIKPTRARANLILRKDGGHAIQEVALRKL"
     gene            complement(2281..2607)
                     /locus_tag="PK812_09435"
     CDS             complement(2281..2607)
                     /locus_tag="PK812_09435"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019905485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HRJ69819.1"
                     /translation="MTGFSLTDLERIVAERAGADADQSYTARLLAGGPARTAKKLGEE
                     ATEAVIAAVSGDRAALTAESADVLYHLLVVLHGAGIPLQDVFGELERRTVQSGLQEKA
                     SRPQGC"
     gene            complement(2604..3389)
                     /gene="hisF"
                     /locus_tag="PK812_09440"
     CDS             complement(2604..3389)
                     /gene="hisF"
                     /locus_tag="PK812_09440"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HRJ69820.1"
                     /translation="MLKIRVIPCLDVKDGRVVKGVQFVELADVGDPVEAARAYDAAGA
                     DELCFLDITASHENRGTLLDIVTRTAENCFMPLTVGGGVRTVEDIRALMLAGADKVSI
                     NTAAVKDISIVGRAAERFGAQAIVVAIDAKQVSAEGEPKRWEIFTHGGRTRTGIDALA
                     FARQAVNLGAGEILLTSMDRDGTKAGFDLDLTRAISDAVPAPVIASGGVGTLDHLVDG
                     VTTGRASAVLAASIFHYGTFTIRQAKEHMAAAGLPMRLDPEGE"
     gene            complement(3409..3876)
                     /locus_tag="PK812_09445"
     CDS             complement(3409..3876)
                     /locus_tag="PK812_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1772 domain-containing protein"
                     /protein_id="HRJ69821.1"
                     /translation="MTLAAMFCGIALYINLAEQPARLALADAPLLEQWKVSFGVALLL
                     QSALAMASGVAGLATWWTHRDWRWAAGGLLMLANWPWTLLVIAPINNALLAISAASAG
                     PVSRALIEQWGVVHAGRTAISIVAVALFISAIARSIKEATGMTRRQKAGMGGI"
     gene            complement(3903..4403)
                     /locus_tag="PK812_09450"
     CDS             complement(3903..4403)
                     /locus_tag="PK812_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HRJ69822.1"
                     /translation="MAIALHWTTAALIIGLMISGFRAASTIDIAEKASLLRIHAPVGM
                     AVLALTLARIAWWRLADVKPLEPAGMSRLQAIGAKAVHGLLYAVILGMVGSGIALFVM
                     SGAGAILAGNAPGPLPDFWSFPPRYGHATMVRLMLLLLLLHLGAALYHQFIRKDRLFA
                     RMGVGQ"
     gene            complement(4417..4902)
                     /locus_tag="PK812_09455"
     CDS             complement(4417..4902)
                     /locus_tag="PK812_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRJ69823.1"
                     /translation="MTSPAVLLTELVLETFRLNGEVLISGDRLVADLRLTSARWQVLG
                     AIGLSTVALPVAHIARNMGLSRQAVQRLVNEMAHQGLVRLAPNPHHQRAKLVLLTAEG
                     AATYAAAMDRQRPWVEALATGLSTEEIGAACRVLRHLRSKLRSQNDRMPEEVRNVEKL
                     T"
     gene            5347..>7271
                     /locus_tag="PK812_09460"
     CDS             5347..>7271
                     /locus_tag="PK812_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HRJ69824.1"
                     /translation="MSRTEMDALGWDSCDIILVTGDAYIDHPSFGMALVGRLLEAQGF
                     RVGVIAQPDWRDASAFRALGKPNLYFGVTSGNMDSMVNRYTSDRRLRHNDSYTPGGEG
                     GKRPDRSVIVYAQRCREAYPEAPVILGGIEASLRRIAHYDYWSDKVRRSILMDAKADL
                     LLYGNAERALVEVSHRVAKGGIRQDFTDVRGVAFVRDRTPEGWLEAPADDLDSPDEGV
                     RRKHMQDQPTVLRMPSYEQVAEDREAYARGSRVLHKEANPGNALPLIQRHGDKEVWLT
                     PPPVPLTTPEMDGVYDLPFARAPHPSYKGQKIPAWEMIRHSVTIMRGCFGGCSFCSIT
                     EHEGRIIQSRSEESILKEIETIRDRTEGFTGIISDIGGPTANMYRIGCKDRETEALCR
                     RPSCVFPDICKNLNTSHDPLIQLYRKARAVPGVRKVNVASGVRYDLAVESPEYVKELV
                     EHHVGGYLKIAPEHTEDGPLSKMMKPGIGTYDAFKKLFDAAAKKAGKKYFLIPYFIAA
                     HPGTTDEDMLNLALWLKKNGYRADQVQTYLPSPMALSTAMYHTGFNPLRPVRRGASEP
                     VDTVRGLRQRRLHKAFLRYHDAENWPLLREALKAMGRADLIGPGKHQLVPGWQPAGTG
                     QRGEGMRIGHKGKQRGPA"
BASE COUNT         1292 a         2408 c         2361 g         1210 t
ORIGIN      
        1 catggcgcgc tcgtcgcctt catccaccgg gcgcaggcct ccggcgcgtc gctcgtggtg
       61 atcgtcaccg gcaagggcgc gccgggcttg gattccgaac gcggcgtgct ccgccggctg
      121 gtgccgcact ggctggccga tccggcgttg cggcgctgca tcgtcggatt cgagccggcg
      181 gggcgcggcc atggcggtga cggcgctctc tatgtgcgca tccgccgccc ttgacgcctc
      241 ggtccgcccc gtgcagggga gcggccgaca taaagacatg gacggaccac atgaccgcac
      301 agttcgacat tctcggcatc ggcaacgcca tcgtcgacgt catcgcccat tcggacgagg
      361 cgtttctcga acgtttcaag gtggcgaagg gatcgatgac cctgatcgac gccgaactcg
      421 ccgagacgct ctatgcggcg atggggccgg ccaccatcat gtccggtggt tcgggcgcca
      481 acacggcggc cggcgccgcc tcgtttggcg cgcgcgttgc ctatgtcgga aagatcaagg
      541 ccgatgcggt gggcgacatc tttgcccatg acatcggggc gcttggcgtt tcctacacga
      601 ccaggcgcgc cgaatccgga ccgccgaccg cgcgctgctt cgttctggtg accccggatg
      661 gcgagcgcac catgaacacc tatctcggcg cctgccagaa tctgagcgag cgcgacatcg
      721 atcccgaggt cgtcggcggc gccaaggtga cctatctcga aggctatctc tgggatccgc
      781 cggaagccaa gaaggccttc cgcaaggccg ccgatattgc ccacaaggcc ggccgcgaag
      841 tgtctttgac cctgtctgat tccttctgcg tcgaccgcta ccgcgacgag tttcgcgatc
      901 tcgtgcgtac cggcatggtg gatctgctct tcgccaatga gagtgaactg aaggcgcttt
      961 atcagaccgc cgacttcgac accgcgctcg ccgccctgcg ccaggaggcg aacaaggccg
     1021 tggtcacccg ctcggagaag ggcgcgatgt tcgtctcgcg cgccgagacc gtggcggttc
     1081 ccgcctatcc gatcgagcaa ctggtggaca cgaccggcgc gggcgatctt ttcgccgccg
     1141 gcttcctgca cggctacact gcggggcgct ccagcgccga ttcgctgcgc ctcggcgcgc
     1201 tggcggcgag ccacatcatc cagcagctcg gcgcccgccc gcaggtggag ctgaagcagc
     1261 tggcgcgcga caacggcctg gggtgagcgc cggccctaca gcttgcgcag cgccacttcc
     1321 tggatggcgt gaccgccatc cttgcgcagg atgaggttgg cccgcgcgcg cgtcggcttg
     1381 atgttttccc acaggttctt caggttgatg cgctcccaga tgccgttggc ggtgcgcagc
     1441 gcctcgtctt ccgccagttc ggcgtatttg cggaagaaag acttgggatc gcggaaggcc
     1501 gtttcgcgca ggcgcatgaa acgggccaca taccagcgcc gcagatcctc ctcgtcggca
     1561 tcgacataga cggtgaagtc gaagaagtcg gagacgaagg ggatggcgcg cccgtccttg
     1621 gcgggtcgcg gcccctgcag cacgttgatg ccctcgaaga tgaggatgtc cggccggtcg
     1681 accacgatct tctcgtccgg cacgatgtcg tagacgaggt gggaatagac cggcgcctcg
     1741 acgttgcgct ggccggcctt cacctgcgcc aggaacttga tcagcgcagc gccgtcgtag
     1801 ctctcgggaa aacccttgcg ctccatcagg ccctgccgct gcaattcggc gttcggcagc
     1861 aggaacccgt cggtggtgac gagttccacc ttcggcgtgt tcggccagcg cgacaacagc
     1921 gccttcaaga cgcgcgcggt ggtggacttg ccggcggaga cggagccggc cacggcgatg
     1981 atgtagggaa ccttgccgtc ctcggccagg aggaagcgct gggtcgcctt gaacagcccc
     2041 tgcgtcgccg cgacatagag tgacagaagg cgtgacagcg gcagatagat ctcgatcacc
     2101 tcgtcgaggg agatcggatc gttgagggat tgcagcttct tcaggtcggc ggcggtcagc
     2161 gtcagcggtg tgtcagcgcg caggttcgcc cattcctcgc gcgagaacac ccgatagggc
     2221 gacaggtcga ccgccgaacc aagctgcgag gctttcgcgt ccatgacacc cccccgagcg
     2281 tcagcagcct tgcggacgcg acgccttttc ctgcaagccc gactggacgg tgcgccgctc
     2341 cagttctccg aacacatcct gaagcggaat gcccgccccg tgaagcacga ccaaaaggtg
     2401 atagagcaca tccgcgcttt cggccgtcag cgcggcacga tccccgctga cggcggcaat
     2461 cacggcttcc gtcgcctcct cgccaagctt cttggccgtg cgcgcgggcc cgcccgcaag
     2521 cagcctggcc gtgtacgact ggtcggcgtc cgctcccgcg cgctcggcca cgatgcgttc
     2581 gaggtcggtc agcgagaaac ccgtcattcg ccctccggat cgagccgcat cggcaggccg
     2641 gctgcggcca tgtgctcctt ggcctggcgg atggtgaagg tgccgtagtg gaagatcgag
     2701 gcggccagga ccgcgctcgc ccgccctgtc gtcacgccgt cgaccaggtg atcaagcgtt
     2761 ccgacgccgc ccgaggcgat gaccggagcc ggcaccgcat cggagatcgc gcgcgtcagg
     2821 tcgaggtcga aaccggcctt ggtgccgtcg cggtccatcg aggtcagcag gatttcgccc
     2881 gcgccgaggt tgaccgcctg gcgcgcgaag gccagggcat cgatgccggt gcgggtgcgc
     2941 ccgccatggg tgaagatttc ccagcgcttg ggctcacctt cggccgagac ctgcttggcg
     3001 tcgatggcga cgacgatggc ctgcgccccg aagcgctcgg cggcgcgccc gacgatggag
     3061 atgtccttca ccgccgcggt gttgatcgag accttgtcgg cgccggccag catcagcgcg
     3121 cggatatcct ccaccgtgcg cacgcccccg cccaccgtca ggggcatgaa gcagttctcg
     3181 gccgtgcgcg tgacgatatc gagcagcgtg ccgcggtttt cgtggctcgc cgtgatgtcg
     3241 aggaagcaga gttcgtcggc gccggcggcg tcgtaggcgc gggcggcctc gaccggatcg
     3301 ccgacatcgg cgagttcgac gaactggacg cccttgacga cgcggccgtc cttcacgtcg
     3361 aggcagggaa tgacgcggat cttgagcatg ctgcaacgct tgtcttcatc agatcccgcc
     3421 catgccagct ttttggcgtc gcgtcatccc tgttgcttcc ttgatggacc gagcaatggc
     3481 cgatatgaac agcgcgaccg ccacgatcga aatcgccgtt cgtccggcat ggacaacgcc
     3541 ccattgctcg atcaacgcgc ggctgacagg tccagcggac gctgcagaga tcgcgagcaa
     3601 ggcattgttg atcggcgcga tcaccagcag ggtccaaggc cagttggcga gcatgaggag
     3661 gccgcccgcc gcccaacgcc agtcgcgatg tgtccaccaa gttgcgaggc cagccacacc
     3721 agaggccatc gccagcgcac tctgcagtag aagtgccaca ccgaagctga ccttccattg
     3781 ttcaaggagc ggagcgtccg cgagagcgag tcgtgcaggc tgctccgcaa ggttgatgta
     3841 aagcgcaatg ccgcagaaca tggccgcgag cgtcaaaacg agcgccggca gtaggcgggc
     3901 aatcattgac cgacccccat gcgtgcgaac agacgatcct tgcgtatgaa ttgatgatac
     3961 agcgctgctc caagatggag gagcagcaag agcaacatca agcggaccat ggtcgcatga
     4021 ccgtagcgag gcgggaagct ccagaagtca ggcaaagggc cgggcgcgtt gccggccagg
     4081 attgcgccag ccccggacat gacgaaaagc gcgatgccgg agccgaccat tccaagaatt
     4141 accgcataga gcagaccgtg taccgccttt gccccgatgg cttgcagacg cgacatacct
     4201 gccggctcca ggggctttac gtcagccaac cgccaccagg cgatccgcgc caaagtcagg
     4261 gcgagcaccg ccatgccaac cggagcgtgg atgcgaagca acgaagcctt ttcggcaata
     4321 tcaatcgtgg aggcggcgcg aaagccgctg atcatcagcc cgatgatcaa cgcggcggtt
     4381 gtccaatgca gcgcaattgc cattcgccca tatcgttcag gtgagctttt ccacattgcg
     4441 gacctcctct ggcatgcggt cgttctggga acgaagcttg gagcgcagat gccgaagcac
     4501 ccggcaagct gcgccgattt cctctgttga aagccccgtt gccagcgctt cgacccaagg
     4561 acgctggcgg tccatcgcgg cggcgtaggt cgcggcgccc tcggccgtca gcaaaaccag
     4621 tttggcgcgc tgatgatgag ggttaggcgc aagccgcacc aagccttgat gtgccatttc
     4681 gttcacgagt ctttgtacgg cctgacggct gagacccatg tttcgggcga tatgggcgac
     4741 aggcagcgcg accgtcgata gaccaattgc tccgagcacc tgccagcgcg cagacgtcag
     4801 gcgcaagtcg gcgacgagcc tgtcacccga aatcagcacc tcaccattga gcctaaacgt
     4861 ctcaaggacg agttcggtaa ggagcacagc gggcgaagtc atgcaatata gtgtcataat
     4921 gacaacatat tgtcaatagg ctgaaggatc gctgtcgtca aggggtcgtt gcacttcgtc
     4981 ctagttcggg agtcctcaag gctttctcgg cgcgggcttc ggcgagctgg atcgcctccg
     5041 cgccgggcaa aggaaatgca gcgtggcgac cgtgagacgg acccacgcgg tatccggtcc
     5101 tgccttgcca aaccgcgccg tcgcgcaatc tctggatgtg cgcttgtgtc gcagctatag
     5161 agcggcacga ccggctgctt ccgccggcgc atcttgcagg cacggatcac catgcacgtt
     5221 tccgccccga ccaatgcctc cgcatccggc gccgcgccgg ccggcgggat cgtgcccacg
     5281 gccaagccgc tgttttccta tcgcaagcac tgggcgcacc gctacgacac ggcgccgttc
     5341 ttcccgatgt cgcgcaccga gatggacgcg ctgggctggg acagctgtga catcatcctg
     5401 gtcacgggcg atgcctatat cgatcatccc agcttcggca tggcgctggt cggccggctc
     5461 ctggaggcgc agggctttcg cgtcggcgtc atcgcgcagc cggactggcg cgatgcctcc
     5521 gccttccgcg cgctgggcaa gcccaacctg tatttcggcg tcacctccgg caacatggat
     5581 tcgatggtga accgctacac ctcggatcgc cggctgcgcc acaacgacag ctacacgccg
     5641 ggcggcgagg gcggaaagcg gccagaccgc tctgtcatcg tctatgccca gcgctgccgg
     5701 gaggcctatc ccgaagcgcc ggtgatcctc ggcggcatcg aggcctcgct gcgccgcatc
     5761 gcccattacg attactggtc ggacaaagtg cgccgctcga tcctgatgga cgccaaggcg
     5821 gacctcctgc tctacggcaa tgccgagcgg gcgctggtgg aagtgtccca ccgcgtggcg
     5881 aaaggtggga tcaggcagga tttcaccgat gtgcgcggtg ttgccttcgt tcgcgaccgc
     5941 acgccggagg gctggctgga ggcgccggcg gacgatctcg acagtccgga cgagggcgtg
     6001 cgccgcaagc acatgcagga tcagccgact gtgctccgca tgccgtccta cgagcaggtg
     6061 gcggaggatc gggaagccta tgcgcgcggc tcgcgggtgc tgcacaagga ggccaatccc
     6121 ggcaacgccc tgccgctgat ccagcgccac ggcgacaagg aagtctggct cacgcccccg
     6181 cccgtgccgc tgacgacgcc ggagatggac ggggtttatg acctgccttt cgcccgcgcc
     6241 ccgcacccgt cctacaaggg ccagaaaatc ccggcctggg agatgatccg ccattccgtg
     6301 acgatcatgc gcggctgctt cggcggctgc tccttctgct ccatcaccga gcacgagggc
     6361 cgcatcatcc agtcacgctc ggaggaatcg atcctgaagg agatcgagac catccgcgac
     6421 cggaccgaag gcttcaccgg catcatctcc gacattggcg gccccaccgc caacatgtac
     6481 cgcatcggct gcaaggatcg cgagacggaa gcgctgtgcc gccgcccgtc ctgcgtcttc
     6541 cccgacatct gcaagaacct caacaccagc catgatcccc tgatccagct ctaccgcaag
     6601 gcgcgggccg tgccgggggt gcgcaaggtc aacgttgcct cgggcgtgcg ttacgacctg
     6661 gccgtggaaa gcccggaata cgtgaaggag ctggtcgagc accatgtcgg cggctatctc
     6721 aagatcgcac ccgaacacac cgaggatggg ccgctctcca agatgatgaa gccgggcatc
     6781 ggcacctacg atgccttcaa gaagctgttc gacgccgcgg cgaagaaggc ggggaagaag
     6841 tacttcctca tcccctattt catcgccgcg caccccggca caaccgatga ggacatgctg
     6901 aacctcgcgc tgtggctgaa gaagaacggc tatcgcgccg accaggtgca gacctacttg
     6961 ccctcgccga tggcgctgtc caccgcgatg taccacaccg gattcaatcc gctcaggccg
     7021 gtgcggcgcg gcgcgtcgga gccggtcgac accgtgcgcg gcctgcgtca gcggcgcctg
     7081 cacaaggcgt tcctgcgcta ccacgatgcc gagaactggc ctctgctgcg cgaggcgctg
     7141 aaggcgatgg gccgcgccga cctgatcggg ccgggaaagc accagcttgt gccgggctgg
     7201 cagccggcgg gcaccgggca gcgcggcgaa ggcatgcgca tcggtcacaa gggcaagcag
     7261 cgtggccctg c
//
LOCUS       DAOMXN010000130         7256 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_5368_length_7256_cov_11.691571, whole genome shotgun sequence.
ACCESSION   DAOMXN010000130 DAOMXN010000000
VERSION     DAOMXN010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 7256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7256
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5368_length_7256_cov_11.691571"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="PK812_09465"
     CDS             complement(<1..485)
                     /locus_tag="PK812_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HRJ69825.1"
                     /translation="MAIPNLNDPFKQRGVTALLGPTNTGKTHFAIERMLGHRSGMIGL
                     PLRLLAREVYQRLTERAGADRVALVTGEEKLLPRSPSYWVSTVEAMPRDLDVSFVAVD
                     EIQLAADADRGHVFTDALLNRRGSEETVLIGSSTIRPLIEELLPGANIIARPRLSKLT
                     FA"
     gene            complement(463..672)
                     /locus_tag="PK812_09470"
     CDS             complement(463..672)
                     /locus_tag="PK812_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69826.1"
                     /translation="MLRYILSGTKQDSRGVADSSKIRSSPVQYVADSRVVTALPVSQG
                     NGTQLFRHARPTDDMDARDGDSEPQ"
     gene            complement(1065..1901)
                     /locus_tag="PK812_09475"
     CDS             complement(1065..1901)
                     /locus_tag="PK812_09475"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HRJ69827.1"
                     /translation="MPVRIKSAQVPGRALVAASFLALTGVPAVAGSIEAQYGISLAGL
                     TLGTATLTGDISKTKYALTARASLTGLAGMVSGGRGAATVTGTIQPQKVVPAAYAVTA
                     ASSDMTRTIQLGMNPGGVHTVVVHPPLEPKADRVPVKPEHQRGVLDPLSAILMPTPVA
                     AGFSGAACNRTIPVFDGAQRFDVQLSYAGMKDVSAEGYSGQVIVCKARYHPVSGHRSE
                     RPATKYMQANRDMEAWLAPTSVSGVFIPFRVSVKTMVGTTVIQAQRFVVGAAQATAKS
                     GN"
     gene            2156..2440
                     /gene="rpmB"
                     /locus_tag="PK812_09480"
     CDS             2156..2440
                     /gene="rpmB"
                     /locus_tag="PK812_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HRJ69828.1"
                     /translation="MSRRCELTGKAVLTGHKVSHSNRKTKRRFLPNLTNVTLISDALG
                     KSVKLRVSTNALRTVEHRGGLDAFLLKADDADLSRDALAVKKELAKKASA"
     gene            complement(2534..3208)
                     /locus_tag="PK812_09485"
     CDS             complement(2534..3208)
                     /locus_tag="PK812_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010658183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRJ69829.1"
                     /translation="MSRTSGVTNEPPLKLREKAYRNFTARLLSRDIRPGQFISQRELV
                     EITGMPLGAIRELVPRLEADGLITTVPQRGMQIAHVDLNLIRNAFQFRMFMEREAAAY
                     FVETAPDAALESLRQQHLEVIERARRGISEDLIRQAQETDWNFHDTIIDHLDNEIVSN
                     AYRVNSIKIRLIRQEQTRLYDALVVPVMEEHLRIIDVFFQRSPQSASAAIAEHIANAR
                     NRAMKL"
     gene            complement(3259..4143)
                     /locus_tag="PK812_09490"
     CDS             complement(3259..4143)
                     /locus_tag="PK812_09490"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRJ69830.1"
                     /translation="MKQTKRFGLSCASATPFRADMTVDAPLLVRHARWLLDQGCDSIT
                     LFGTTGEGASLAPADRIAMLGALDGAGIIMRSQVVAGIMANTPNDADDQLKLAYDAGC
                     RAILLAPPSYFKDVSDDGVFRWVAMLLERAGPRARDIILYHIPQMTGVAFSIALVDRL
                     KKAFPGVIIGVKDSAAIWEQTEALIKAHGDLAILVGAESQLAAAVKLGGQGTICGYAN
                     TIPASLRDVAGKGKDHPDIARMGRVTGRYHFLQEVKAMIAHIHGEPGWLNLRPPLVSV
                     PKAAERAIVKRFRSEFPA"
     gene            4396..5427
                     /locus_tag="PK812_09495"
     CDS             4396..5427
                     /locus_tag="PK812_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRJ69831.1"
                     /translation="MAKIGKRAIGKTGLKVSSLGFGGAPLGDLYALLDEATAVDAVVA
                     AIRSGTTLIDTSPLYGHGLSEHRIGAALRRVARPVVLSTKVGRVTTPARGGRVAGQSY
                     VGGLSFDAAFDYSYDGAMRSLEQSLLRLGRNSVDILLIHDVDVWTHGPAMIEQRFKEA
                     MNGAYRALERLRAEGTVRAIGVGVNEAEMCARFATAGDFDCMLLAGRYSLLEQGALDT
                     FLPLAERRRISVLLGGVFNSGILATGAVPGAKYNYQAAPPEILERVRRIEEVCVAHRV
                     PLAAAAVQFPLAHPAIASVVLGAVTPAEVERNLAGVSRRIPPRFWDDLRTAGVLPREV
                     PTPGRAQAS"
     gene            5424..6272
                     /locus_tag="PK812_09500"
     CDS             5424..6272
                     /locus_tag="PK812_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006931369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRJ69832.1"
                     /translation="MKIDAHQHFWRLDRGDYGWLTPALGPIYRDFAPADFQPLLERHR
                     IDASILVQAAPTVAETEFLLETAGRTPFVAGVVGWIDFDAPDAVGQIDRLAANPLLVG
                     VRPMVQDIADDDWLARASHNRLFEMLVDRRLVFDALVLPRHLGRLLGVVERHPALAIV
                     IDHLAKPPIADGRLDPWRADMNRLAAHANVACKLSGMVTEAGDNWTVERLAPFAAIVL
                     DAFGPQRLLWGSDWPVVDLAGGYERWRSAALQLIRHLPQQGQDAILGATAARVYLGHG
                     RGRQPC"
     gene            6266..6736
                     /locus_tag="PK812_09505"
     CDS             6266..6736
                     /locus_tag="PK812_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019998269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0048029 - monosaccharide binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU domain-containing protein"
                     /protein_id="HRJ69833.1"
                     /translation="MLKGIDPVLSADLLWVLAAMGHGDDLALVDANHPAEAMARHTVS
                     GRLVRLPGLAIERAAAAILSVLPIDDFTPDPVRRMEVVGDPEALPDVQRAVRDVMAAR
                     AGRDIPLTGLERFAFYEAEKKAFAVVQAGDPRPYGCFLFRKGVIAGSRPDAFGH"
     gene            6883..>7256
                     /gene="rplU"
                     /locus_tag="PK812_09510"
     CDS             6883..>7256
                     /gene="rplU"
                     /locus_tag="PK812_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019994905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HRJ69834.1"
                     /translation="MFAVIKTGGKQYRVAANDVITIERLAGEPGDILTFEQVLMIGGS
                     TAIIGSPTVSGACVAGELVEQARGPKTISFKKRRRQNSKRKKGHRQDLSVVRITEILT
                     DGKKPAAKAKAEKPATVEAAAAA"
BASE COUNT         1273 a         2378 c         2357 g         1248 t
ORIGIN      
        1 gcgaacgtca gcttcgacag gcgcgggcgg gcgatgatgt tggcgcccgg caacagttcc
       61 tcgatcagtg gcctgatggt cgaggagccg atcagcaccg tctcctcgct cccgcgccgg
      121 ttcaggagag cgtcggtgaa gacgtgacca cgatcggcat ccgccgccag ttggatctcg
      181 tccaccgcca cgaaggacac gtccaggtcg cgcggcatcg cctcgacggt cgagacccag
      241 taggacggcg aacgcggcag cagcttctcc tcgccggtca ccagcgccac ccggtccgcg
      301 ccggcgcgct cggtgagccg ttgatagacc tcccgcgcca gcagccgcag cggcaatccg
      361 atcatgccgc tccggtggcc gagcatccgc tcgatggcga aatgggtctt gcccgtgttg
      421 gtcgggccca gcagcgccgt cacgccacgc tgcttgaacg ggtcattgag gttcggaatc
      481 gccatctcga gcgtccatat catcggtggg ccgggcgtgg cgaaaaagtt gcgttccgtt
      541 tccctgcgac acgggcagcg cggtcacgac gcgcgaatcg gcaacgtatt gaacgggaga
      601 ggaacgaatc ttggacgaat cagcgactcc ccgtgagtct tgttttgttc ctgacaagat
      661 atagcgcagc atggtggact catcgccaaa tctagcgttt ccgccaagtc ttcagagaaa
      721 tacggcggac agcgcacagg actcgcagga cgcaagaggg actcctgtgt caagccggaa
      781 agactcgaaa tcatggttaa cgaattggaa aactactggt tcgagatcgc ctcaggagcg
      841 gtgaggatcg caccggaacg ggaacgaaac acggacgaat cacttaccgc gagtcgcggc
      901 gtgcctgtac gacaagccaa ggctttgaat tgtaatgagg aattcgatca gcgcacgggc
      961 atcgcagcgg caccagccgg ccattgcccg catggtgctc ggtcaggaat tcacagtgtg
     1021 agccgaaacg acacagcccg cgccctggag cgcgcggcgc gccttcagtt gccggacttg
     1081 gcggtggcct gcgcggcgcc gacgacaaac cgctgggcct gaatgaccgt cgtcccgacc
     1141 atggtcttca ccgagacccg gaacggaatg aacacgccac tcaccgaggt cggggcaagc
     1201 caggcttcca tgtcccggtt ggcctgcatg tacttcgtgg ccgggcgctc cgaccgatgg
     1261 ccagacaccg gatgatagcg cgccttgcag acgatgacct gcccggaata gccctcggcg
     1321 gaaacgtcct tcatcccggc atagctgagc tggacatcga accgctgggc cccgtcaaag
     1381 accggaatgg tgcgattgca ggcggccccg ctgaagcccg ccgccaccgg cgtcggcatc
     1441 aggatggcgc tgagcggatc gagcacgcca cgctgatgct ccggcttcac cggaacgcgg
     1501 tcggccttgg gctcaagcgg tggatgcaca accacggtat gcacgccgcc ggggttcatg
     1561 ccgagctgga tcgtgcgcgt catgtcgctg gaggcggccg tgacggcata ggccgccgga
     1621 acaaccttct gcggttggat cgtaccggtc accgtcgccg cgccgcgacc gccggaaacc
     1681 atgccggcga gaccggtcag actggcgcgg gcagtcagcg cgtacttcgt cttggaaatg
     1741 tcgccggtca gtgtcgcggt gccgagcgtc aggccggcaa gcgaaatgcc atactgggcc
     1801 tcgatgctgc cggccacggc cggaacaccg gtgagcgcca gaaaggacgc ggcgaccagt
     1861 gcacgaccgg ggacttgggc agatttgata cgcacaggca tgatcggttt ccggattccc
     1921 gcagcggctt tgccgcgttc tggcacgact ggactgccat cctatgcaag gattggttaa
     1981 ccgtaaatgt atggcgtgtt gcagcatcga catcagggtt tgcgcttgac cgcggacagg
     2041 cttgccccta tacgcgcggc ttcgatccgg atcattctgg ctggactgcg tcctcggagc
     2101 ccacttcgcg gcttgttttc gttgtctcca gggaacagtt ggaaaggcgt aacccatgtc
     2161 gcgtcgctgc gaactcacag gcaaagcggt cctcaccggc cacaaggtca gccattcgaa
     2221 tcgcaagacc aagcgcaggt tcctgccgaa cctgaccaat gtgacgctga tctccgatgc
     2281 tctcgggaag tcggtgaagc tgcgtgtctc caccaacgcg ctgcgcacgg tcgagcatcg
     2341 cggcggcctc gacgccttcc tgctcaaggc ggatgacgcc gacctgtccc gcgacgcgct
     2401 cgcggtgaag aaggaactgg cgaagaaagc ttcggcctga gccaattcgc gcagcggaac
     2461 ggaaccggcc cggcaagggc cggttttttg tgtttctaga gcactatccg accagatcgg
     2521 atcggatatc gctctacagt ttcatcgccc gatttcgggc gttggcgatg tgttcggcga
     2581 tggccgcgga ggcgctttgc gggcttcgct ggaagaacac gtcgatgatc cgcaggtgtt
     2641 cttccatgac cggaacgacg agcgcatcat agaggcgggt ctgttcctga cggatcaacc
     2701 ggatcttgat ggaattcacg cgataggcgt tcgaaacgat ctcgttgtcg agatgatcga
     2761 tgatcgtgtc gtggaaattc cagtcggtct cctgcgcctg cctgatcaga tcctcgctga
     2821 tgccgcgccg cgccctctca atcacctcca ggtgctgttg ccggagactt tccagcgcgg
     2881 catccggtgc cgtctcgacg aaataggcgg cggcctcccg ttccatgaac atccggaact
     2941 ggaaggcgtt gcgaatgagg ttcaggtcca catgggcgat ctgcatgccc cgctggggca
     3001 ccgtggtgat cagcccgtca gcctccaggc gcggaaccag ttcccgaatc gctcccaggg
     3061 gcatgcccgt gatctcgacc agttcgcgct gggagatgaa ctggcccggc cggatgtcgc
     3121 gcgacagcag gcgcgccgtg aagttccggt acgccttctc gcgaagcttc aggggtggtt
     3181 cgttcgtcac gcccgacgtc cggctcatgg ctcctccgcc ctgcttgctc cacccgcgac
     3241 cgctcctggc ggccgccctc acgcggggaa ttcgctccgg aaccgcttga cgatcgcccg
     3301 ctccgccgct tttggcaccg acaccagcgg cggccgcaga ttcagccagc ccggctcccc
     3361 gtgaatgtgg gcgatcatcg ccttcacctc ctgcaggaaa tggtagcggc ccgtgacgcg
     3421 tcccatgcgg gcgatgtcgg gatgatcctt ccccttgccg gcgacgtcgc gcaggctggc
     3481 cgggattgtg ttggcatagc cgcaaatcgt gccctgtcca ccgagcttca ccgccgccgc
     3541 tagctgcgat tcggccccga ccaggatcgc gagatcgcca tgggccttga tcagggcttc
     3601 cgtctgctcc cagatcgcgg ctgaatcctt gacgccgatg atgacaccgg gaaacgcctt
     3661 cttcagccga tcgaccaggg cgatggagaa ggccacgccg gtcatttgcg ggatatgata
     3721 caggatgatg tcgcgcgccc gcggcccggc ccgctccagc agcatcgcca cccagcggaa
     3781 cacaccgtca tcgctcacat ccttgaaata ggacggaggc gccagcagga tggcgcggca
     3841 gccggcgtca taggccagtt tcagctgatc gtcggcatcg ttcggcgtgt tcgccatgat
     3901 gcccgccacg acctgggacc gcatgatgat gccagcgccg tcgagcgcgc ccagcatggc
     3961 gatgcggtcg gccggtgcca gcgaagcgcc ttcgcccgtg gttccgaata gcgtgatgct
     4021 gtcacagccc tggtcgagca gccaccgcgc atggcgaacc agcaacggcg catccacagt
     4081 catatcggcg cgaaatggcg tcgcggatgc acaggacagt ccaaaacgct tcgtctgctt
     4141 catgatcggc cagcctccaa ggaacgtaac atgttagaga gcgcggcggg acaagtaaac
     4201 tgcccaacag catacgttcc gatcggtgcg aaaacgggcc ggagcgcgga ggcgccttgt
     4261 ggttgctccc cgcatgatgc gatccgaacc gctccaggag gtggcagacg acagcaatgc
     4321 gttaacatgt cagcttcctc caagcttgcg aatccggtgc agatgaacaa tcattcatcc
     4381 cagtggagtg ccagcatggc aaagatcggc aagcgggcga tcggcaagac agggctgaag
     4441 gtttccagcc tcggattcgg cggcgctccg ctgggcgatc tctacgcgct gctcgatgag
     4501 gccacggccg tggatgcggt ggtcgcggcc attcgctccg gcacgaccct gatcgacacc
     4561 tcgcccctgt acggacacgg actttccgaa caccggatcg gcgcggcgct ccggcgggtg
     4621 gcgcggcccg tcgtgctcag cacgaaggtc gggcgcgtga cgacgccagc ccggggcggc
     4681 cgggtcgccg gccagagcta cgtcggcggc ctgagtttcg atgccgcatt cgactattcc
     4741 tacgacggcg cgatgcgctc gctcgaacaa tcactgctgc gactgggccg caacagcgtg
     4801 gacatcctgc tgatccacga tgtcgacgtc tggacgcatg gccccgccat gatcgaacag
     4861 cggttcaagg aggccatgaa cggcgcctac cgggcgctgg aaaggttgcg cgccgaggga
     4921 acggtcaggg ccatcggtgt cggcgtgaac gaggcggaga tgtgcgcgcg cttcgccacg
     4981 gcgggcgact tcgactgcat gctcctcgcc gggcgctact ccctgctcga gcagggcgca
     5041 ctggacacct tcctgccact cgcggaacgc cgccgcatct cggtgctgct cggcggcgtg
     5101 ttcaattccg gcatccttgc aaccggtgcc gtgccgggcg ccaaatacaa ctatcaggca
     5161 gcgccgccgg agattctgga acgggtgcga cggatcgagg aggtctgcgt tgcccatcgc
     5221 gtgccgctcg cggccgccgc ggtccagttc ccgcttgccc atcccgccat cgcctcggtt
     5281 gtgctgggcg cggtgacgcc cgccgaagtc gagcgaaacc tcgccggagt ctcgcgccgc
     5341 atcccgcccc gattctggga tgacctgcgc acggcggggg tgctgccgcg cgaggtgccg
     5401 acgccggggc gggcgcaagc gtcatgaaga tcgacgcgca tcagcatttc tggcggctgg
     5461 atcgcggcga ctacggttgg ctgacgccag ctcttgggcc gatctaccgg gacttcgcgc
     5521 ccgccgactt ccagcccctg ctggagcgcc accgcatcga cgccagcatc ctcgtgcagg
     5581 ccgcaccgac agtcgccgaa actgagttcc tgctggagac cgccggacga accccgttcg
     5641 tcgctggtgt ggtcggctgg atcgacttcg atgcgccgga tgccgtcggg cagatcgacc
     5701 gcctcgcagc caacccgctg ctggtcggtg tccggccgat ggtgcaggac atcgccgacg
     5761 atgactggct ggcgcgcgcc agccataacc ggctgttcga aatgctcgtc gaccggcggc
     5821 tggtgttcga tgccctggtc ctgccccggc atctcggccg ccttctgggc gtcgtcgagc
     5881 ggcatccggc gctcgcaatc gtcatcgacc atctggcgaa accaccgatc gcggacggcc
     5941 gtctcgatcc ctggcgcgcc gacatgaaca ggctcgccgc ccatgccaac gtggcctgca
     6001 aactgtccgg gatggtgacc gaggcaggcg acaactggac ggtcgagcgc ctggcgccgt
     6061 tcgccgccat cgttctcgac gccttcggtc cgcagcgcct cctgtgggga tccgactggc
     6121 cggtcgtcga cctggccggc ggctacgagc gctggcggtc ggcggcattg cagttgatcc
     6181 gccatctgcc gcaacagggg caggacgcca ttcttggcgc cacggcggcg cgagtctatc
     6241 tcggccacgg acggggaagg cagccatgct gaagggcatc gatccggttc tttccgccga
     6301 cctgctctgg gtactggccg ccatgggcca cggcgacgat ctggcgttgg tcgacgcgaa
     6361 ccatccggcc gaggccatgg cgcggcacac cgtttccggc cgccttgtcc gccttcccgg
     6421 cctggcgatc gaacgtgccg cagcggccat tctgtcggtc ttgcccatcg acgatttcac
     6481 ccccgatccc gtgcgtcgca tggaggtcgt gggcgaccct gaggccctgc ccgacgtgca
     6541 gcgggcggtc agggatgtga tggcggcgcg cgccgggcgc gacatcccgt tgaccggcct
     6601 ggagcgcttc gccttctacg aagccgagaa gaaggccttc gccgtggtcc aagccggcga
     6661 cccgcggccc tatggctgtt tcctcttccg caagggcgtt atagcaggca gccgtcccga
     6721 cgcgttcgga cattgacagg taccggcctt tcggcctaga gagccgccgt cgcgcggcgg
     6781 aaacggccgc gcgttgcgtt tgacgcacaa gaaccagcaa aaagacgacc gggatcgctg
     6841 ctttccttgg aggcgcggcc acggacgtga aacgaggtag acatgttcgc agtgatcaag
     6901 accggcggca agcagtatcg cgtcgccgcc aacgacgtca tcaccatcga gcgcctggct
     6961 ggcgagccgg gcgacatcct gaccttcgag caggtgctca tgatcggcgg ctcgaccgcg
     7021 atcatcggct ccccgactgt ctccggtgcc tgcgtggcgg gcgaactggt cgagcaggcg
     7081 cgtggcccga agacgatctc cttcaagaag cgtcgtcgcc agaattccaa gcgcaagaag
     7141 ggccatcgcc aggacctttc ggtggtccgg atcaccgaga tcctgaccga cggcaagaag
     7201 cccgccgcga aggccaaggc cgagaagccg gcgacggttg aagcggctgc ggctgc
//
LOCUS       DAOMXN010000131         7213 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_5404_length_7213_cov_8.924141, whole genome shotgun sequence.
ACCESSION   DAOMXN010000131 DAOMXN010000000
VERSION     DAOMXN010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 7213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7213
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5404_length_7213_cov_8.924141"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2767)
                     /locus_tag="PK812_09515"
     CDS             complement(<1..2767)
                     /locus_tag="PK812_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ69835.1"
                     /translation="MADEVSSQSLSQILGDLAPFNPLKDWVDLTHAEIRKLFEAEDFK
                     KACCDYIEECGVRKPMNKGVYWCLEKCVFAAGSILGDKGKKVLLGVLEKTPGVSTLFA
                     RILQIARKSQEAEQVRQALLNDLRARRPKLGGEDVTLDHTSERQIMEFLYLKESLDGL
                     PEAVRKQLESNFGELRDAIAAQFQDMFQPQLTWPQSLNHGGGIIDGLMYANERDPFVG
                     REEELDLLSRLLGTPDQWTPGNRFRWLALVGPGGEGKSRLAMHFSKEMAQARGWDHGR
                     LSTDDIARLEPAKWRPRKPTLITIDYAARRPDDVRALIIAFAQAQFDYDFPVRLLLLE
                     RDAGAWFARLMARDNDLPAIVNGAFLKEPHVAPVDPVMQDKIIDLMQGRFQRAGIPAP
                     EPAHLLKAAYSVDRRAFKTDEGILPLPRPLFAAAAAEAMIELTRQGAEPSKLAAELDR
                     DTVLAGLIARDRNALWEPACRGKDGTVDEAKLALHENLLLVTTIAQGLPEAPLRKDCP
                     QAARDYLPDFGPKGAHPLNLRVVERMGGRTDGVLGQLEPDIVGEFFVLNRLAKLENSD
                     PDAKAALIDAALALGQDDSGIFILRCFGDFQERTERLDFLRPTHIDAAFRPWSRALAV
                     VGDHYATYANLEAALRTYESALEAARRSGDERDLSISHDRIGDVRVEQGDLTGALTAY
                     AASHAIAERLAAADRSNTLWQRDLSISHDRIGDVRRAQGDLAGALTAYEASLAIRQRL
                     AAADRSNTEWQRDLSVSHNRIGDVRMAQGDLAGALTAYEADLAIAEHLAAADRSNTQW
                     QRDLSISHNRIGDVRTAQGDLAGALTAYAASLAIAERLAAADQSNTLWQRDLSISHTK
                     IGDVRTAQGDLADALTAYEASLAIFERLAAADRSNTEWQRDLSISHEKIGDVRRAQGD
                     LTGALTAY"
     gene            3062..3490
                     /gene="rplK"
                     /locus_tag="PK812_09520"
     CDS             3062..3490
                     /gene="rplK"
                     /locus_tag="PK812_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HRJ69836.1"
                     /translation="MAKKIVGFIKLQVPAGAANPSPPIGPALGQRGLNIMEFCKAFNA
                     KTQQMEKGTPIPVIITAYQDRSFTFEMKQPPVSYFLKKAASLTSGSKTPGRGFVGKVS
                     MAQINEIAAKKMADLNCDSVESAASMIAGSARSMGLEVVG"
     gene            3495..4193
                     /gene="rplA"
                     /locus_tag="PK812_09525"
     CDS             3495..4193
                     /gene="rplA"
                     /locus_tag="PK812_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018099704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HRJ69837.1"
                     /translation="MANVSKRTQKAREGVERTKLYPLSEAVKMIKARASAKFDETIEI
                     AMNLGVDPRHADQVVRGVCNLPNGSGRKVRVAVFARGPKADEAKAAGADIVGAEELFE
                     KVNGGTIEFDRCIATPDMMGLVGRLGKVLGPRGLMPNPRVGTVTMDVTNAVKGAKGGS
                     VEFRAEKAGIVQGGVGKASFTEAQLEQNIRAFADAVIKAKPTGAKGQYLLRAAISSSM
                     GPGVKVELASLNAQ"
     gene            4475..5416
                     /locus_tag="PK812_09530"
     CDS             4475..5416
                     /locus_tag="PK812_09530"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRJ69838.1"
                     /translation="MASLSNFSSFKDTNLSKENFDKIYVQPDPREYYRVLYGLDYVIP
                     ELAKPIFRNIVNALQEQEQRPIKVLDVGSSYGINAALLRFPIDLDRLGARYRDLSLSD
                     IDREELVWHDRHYFLSWPAADVEVIGLDVSESAIRYAKEVGLISDGIVANLEERMLTD
                     QEAEMLSDVDLIISTGCVGYIGERTFSKLLAAMDRTPWVASFVLRMFDYGELEGVFSS
                     HELSTEKLKGVTFVQRRFNSYDEYTSVVSKLLETGVRVDGKESEGLLHAEFYLSRPRD
                     ASVELPLEQIAQVTSGANRRYGRRFRMTPAQTLRFGV"
     gene            6350..6868
                     /gene="rplJ"
                     /locus_tag="PK812_09535"
     CDS             6350..6868
                     /gene="rplJ"
                     /locus_tag="PK812_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015857356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HRJ69839.1"
                     /translation="MDRAGKKELVSTLNSVFKDTGAIVVAHYSGLTVAQMQKLRKEMK
                     AAGASVKVAKNRLVKIALEGTDVASIGSLMKGPTILAYSKDPVAAPKVAVAFAKDHDK
                     LVILGGAMGTTALKPDGVKALATMPSLDELRAKLVGLIQAPATKIAQLQTAPAAKLAR
                     VFGAYAKRDEAA"
     gene            6930..>7213
                     /gene="rplL"
                     /locus_tag="PK812_09540"
     CDS             6930..>7213
                     /gene="rplL"
                     /locus_tag="PK812_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003597539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HRJ69840.1"
                     /translation="MTDLSKIVDELSKLTVLEAAELAKMLEEKWGVSAAAAVAVAAAP
                     GGGGAAAPVEEQTEFTVILASAGDKKIEVIKEVRAITGLGLKEAKDLVEGA"
BASE COUNT         1355 a         2185 c         2298 g         1375 t
ORIGIN      
        1 cgtacgccgt cagcgcgccg gtaagatcgc cctgcgcgcg gcggacatcg ccgatctttt
       61 catgggagat cgagagatcg cgctgccact ccgtgttgct ccggtcggcg gcggcgaggc
      121 gttcgaagat ggcgaggctg gcctcgtacg ccgtcagcgc gtcggcgaga tcgccctgcg
      181 cggtgcggac atcgccgatc ttggtgtggg agatcgagag atcgcgctgc cacaaggtgt
      241 tgctctggtc ggcggcggcg aggcgttcgg caatggcgag gctggccgcg taagccgtca
      301 gcgcgccggc gagatcgccc tgcgcggtgc ggacatcgcc gatcctgttg tgggagatcg
      361 agagatcgcg ctgccactgc gtgttgctcc ggtcggcggc ggcgaggtgt tcggcaatgg
      421 cgaggtcggc ctcgtacgcc gtcagcgcgc cggcgagatc gccctgcgcc atgcggacat
      481 cgccgatcct gttgtgggag accgagagat cgcgctgcca ctccgtgttg ctccggtcgg
      541 cggcggcgag gcgttggcga atggcgaggc tggcctcata cgccgtcagc gcgccggcga
      601 gatcgccctg cgcgcggcgg acatcgccga tcctgtcgtg ggagatcgag agatcgcgct
      661 gccacaaggt gttgctccgg tcggcggcgg cgaggcgttc ggcgatggcg tgactggccg
      721 cgtacgccgt cagcgcgccg gtaagatcgc cctgctcgac gcggacatcg ccgatcctgt
      781 cgtgggagat cgagagatcg cgctcgtcgc cgctgcgccg ggccgcttcg agggcggact
      841 cataggtgcg cagagcagcc tcgaggttcg cataggtcgc gtagtggtcg ccaaccactg
      901 ccagggctcg ggaccatggc cggaacgcgg cgtcgatgtg tgtcgggcgc aggaaatcga
      961 gccgttcggt gcgctcctga aagtcgccga agcaccgcag gatgaaaatg ccggaatcgt
     1021 cctgccccaa cgccagagcg gcgtcgatca gcgcggcttt cgcatcgggg tccgagtttt
     1081 caagcttcgc gagccggttc aggacgaaga actcgccgac gatgtccggc tccaactgtc
     1141 ccagaacgcc gtctgtcctg ccgcccatgc gctccacaac gcgaaggttc agcgggtgcg
     1201 cgcccttcgg gccgaaatcc ggcaggtaat cgcgcgctgc ctgtgggcaa tccttccgca
     1261 acggcgcttc cggcaggccc tgcgcgatgg tggtcaccag aagcaggttt tcatgcagcg
     1321 cgagcttggc ttcgtcgaca gtcccgtcct tgcccctgca ggccggctcc cacagcgcgt
     1381 tgcggtcgcg cgcgatcaac cccgccagca ccgtgtcgcg atccagttcc gccgcgagct
     1441 tggacggctc ggcgccttgc cgcgtaagct cgatcatcgc ttcggccgct gcggcggcga
     1501 acagcggtct tggcagaggc aaaataccct cgtccgtctt gaaagcacgc cggtcgacgg
     1561 aataggcagc cttcaacaag tgcgccggtt ccggcgcggg aatgccggca cgctgaaagc
     1621 gcccttgcat caggtcgatg atcttgtcct gcatgaccgg gtccaccggc gcgacatgcg
     1681 gctctttcag aaaagcgccg ttgacgatgg ccggcagatc gttgtcgcgc gccatgagcc
     1741 tcgcgaacca ggcccccgcg tcacgttcca ggagcagcag gcgcactgga aaatcatagt
     1801 cgaactgcgc ctgcgcgaag gcgatgatca gggcgcgcac gtcatcgggg cggcgggcgg
     1861 cgtagtcgat cgtgatcagc gtcggcttgc gcggccgcca tttcgcgggc tccagcctgg
     1921 cgatgtcgtc cgtcgaaagc cgcccgtggt cccagccgcg cgcctgcgcc atctccttgg
     1981 aaaaatgcat ggcgaggcgg ctcttgcctt ctccgccggg accgaccagc gcaagccagc
     2041 ggaagcgatt gccgggtgtc cactggtccg gcgtgcccag aagccggctc agcaggtcca
     2101 gttcttcctc acgcccgaca aacggatcgc gttcgttggc gtacatcaat ccgtcgatga
     2161 tgccgccgcc gtggttcagc gactgcggcc aggtcagctg cggctggaac atgtcctgaa
     2221 actgcgccgc gatagcgtcg cgcaactcgc cgaagttgct ttccagctgc ttccggacag
     2281 cctcgggcag gccgtcgagg ctctccttga ggtacaggaa ctccatgatc tggcgctcgc
     2341 tggtgtgatc gagcgtgacg tcctcgccgc cgagtttcgg ccgccgtgcg cgcagatcgt
     2401 tcagcaaggc ctgccggacc tgttcggctt cctgagactt gcgggcgatt tgcaggatgc
     2461 gcgcaaataa tgtgctgacg ccaggggtct tctccaaaac ccccaggagg accttcttgc
     2521 ctttatcgcc gagaatgctg cctgcggcga acacgcattt ctcaaggcac cagtaaacgc
     2581 ctttgttcat aggctttctg acgccacact cttcgatgta gtcgcagcag gccttcttga
     2641 aatcctcggc ttcgaacaac ttgcggattt ccgcatgcgt cagatcgacc caatccttga
     2701 gcggattgaa cggggccaaa tcgcccaaaa tttgcgagag actctgtgaa gagacttcgt
     2761 ccgccatggg tgaccccttg gatttgacaa caaaatacgc atcgcgacgt catttgttca
     2821 agtatcgcgc gggagatatt cgcagatttt cgggatcaca gttccccttg ccatctcctc
     2881 cattcccgcc tatacgcgcc gcttctccgg ggagttccgc tcgcccggag tgaacgcgtg
     2941 ggaggcggtt cgccggggtg tctcgccccg tcagacacaa gcccgaccac gatcctgaac
     3001 ccgcctgcgg gtcgtcttcc ttcaggggaa gcgcggcctc acggcatttt ggaagagaac
     3061 gatggccaag aaaatcgtcg gcttcatcaa gcttcaggtt ccggctggcg ccgcgaaccc
     3121 atccccgccg atcggtcccg cgctcggtca gcgcggcctg aacatcatgg aattctgcaa
     3181 ggccttcaac gcgaagaccc agcagatgga aaaggggacg ccgatccccg tcatcatcac
     3241 ggcctaccag gaccgctcct tcaccttcga gatgaagcag ccgccggtga gctatttcct
     3301 caagaaggcg gcgagcctga cctccggctc caagacgccg ggccgcggct tcgtcggcaa
     3361 ggtctccatg gcgcagatca atgagatcgc cgccaagaag atggccgatc tcaactgcga
     3421 cagcgtcgaa agcgccgcgt cgatgatcgc cggttccgcc cgttcgatgg gcctcgaggt
     3481 ggtggggtaa gaccatggcc aacgtttcca agcgtaccca gaaggcccgc gagggcgtcg
     3541 agcgcaccaa gctctatccg ctctccgaag cggtgaagat gatcaaggcc cgcgcctcgg
     3601 ccaagttcga cgagacgatc gagatcgcca tgaacctcgg cgtcgatccg cgccatgccg
     3661 atcaggtcgt gcgcggcgtc tgcaatctgc cgaacggctc cggccgcaag gtgcgcgtcg
     3721 cggtgttcgc gcgcggcccg aaggcggacg aggcgaaggc cgctggcgcc gacatcgtcg
     3781 gtgcggaaga gctgttcgag aaggtcaacg gcggcacgat cgagttcgac cgctgcatcg
     3841 ccacgccgga catgatgggc ctcgtcggcc gtctcggcaa ggtgctcggc ccgcgcggcc
     3901 tgatgccgaa cccgcgcgtc ggcaccgtga ccatggacgt caccaacgcc gtgaagggcg
     3961 ccaagggcgg ctcggtcgag ttccgcgccg agaaggccgg catcgtgcag ggtggcgtcg
     4021 gcaaggcctc gttcaccgag gcgcagctgg agcagaacat ccgcgccttc gccgatgcgg
     4081 tgatcaaggc caagccgacc ggcgccaagg ggcagtatct gctgcgcgcg gccatctcgt
     4141 cctcgatggg gccgggcgtg aaggtcgagc tggcgagcct caatgctcag taaggtcggc
     4201 tgagagccga ccgcaagaca ggcaagaccc cggcggaaac gtcggggttt tttgttgcaa
     4261 ttgacgtccc ggcccttcac gggaacgaaa ctcgcaaccc ggaatcctgg ccggaaacag
     4321 tcctggaatg caagatattg gcgtttgaat ccgccatatt ttgaacatag tctttggact
     4381 tcctagcgac ggggtaatca tatcgtcatg gaggataatg tttcgctttc gaaatgagcg
     4441 atcttcaaag caaacatgac aggagggcaa cgtcatggca tcactttcca atttttcatc
     4501 tttcaaggac acgaacttgt cgaaagaaaa ttttgacaag atttatgtac aacctgatcc
     4561 cagagaatat taccgtgttt tatatggact cgactatgtc atacctgagt tggcaaaacc
     4621 tattttccgc aacattgtca acgccctgca ggagcaggag cagcgaccga tcaaggttct
     4681 ggatgtcggc agcagttatg gaatcaacgc ggcactgttg cgatttccaa tcgatctgga
     4741 tcggctgggc gctcgatacc gcgatctgag cttgtcggac atcgaccggg aggaactagt
     4801 ttggcatgat cggcactatt ttcttagttg gccagctgca gatgtcgaag tcatcggtct
     4861 cgatgtgtcc gaatccgcga ttcgttacgc caaggaggtg gggctgattt ctgatggtat
     4921 tgtcgcaaat cttgaagaga gaatgcttac tgatcaagaa gcggaaatgc tttcggatgt
     4981 tgacctgata atatcaacgg gttgtgttgg atatattggc gaaagaacat tctcaaagct
     5041 gctcgctgcg atggatagaa cgccgtgggt cgcatcgttc gtcctaagga tgttcgatta
     5101 cggcgagttg gagggcgttt tttcttctca cgaactttcg acagaaaaat tgaagggcgt
     5161 gacttttgtt caacggcgct tcaattcata cgatgagtat acatctgttg tatcgaaact
     5221 cttagaaact ggcgttcgtg tggatggcaa agaatcggag ggccttcttc atgccgaatt
     5281 ctatctttca cgtcccagag atgcatccgt agaacttccg cttgagcaga tcgcccaggt
     5341 cactagcggt gccaatcggc ggtacggtcg tcgtttcagg atgacacctg cccagactct
     5401 gagatttgga gtttagggcc cacctgaccg gcgcggattt ggcccggccc cctaggggac
     5461 aaccttcttc acctcctgtg gttgcgcctg tgtgcaacca catcctcaat gcggccgggc
     5521 gtgaaggtcg agctggccgg cctcagcact cattatacat cgccagtcgc cgacggcgaa
     5581 tggccaatcg ggaccccggc ggaaactccg gggttttgct tgggtgtcca ggaggcaggg
     5641 gcgtgttgcg ccgcaaagaa accccaattg gccccttgcg actcgtgtct aacccggcta
     5701 taggcccggc catgctcagc gtcatgctgc ccgagcgcgg cggttttccg cgcatcaaca
     5761 gagtttccgc tggcggtaag ccatcaggcg aacgttaagc gggggaatgg gccggaagac
     5821 ggcagggaaa cctgtttgtc ttttccggcc atgtcctgtc cgagactgcg ggtgcgacgg
     5881 gctggagacg aattccttga gggaattcaa tcttcaagcc ggcttaatgc caaaggggct
     5941 cagccccgcc ggagcgatcc ggtgatgacg gcgtccgcat agacggggaa gaccgaattg
     6001 ttcccggttc gtcggttcga cgggccggct gcgtttggtt cgaacctctt gtccttgggg
     6061 ccggcggatt ttccgcctct ccggggggac aggcaactcg gggcttggag gcgaaagccg
     6121 aaagcccctg ggtgatgaca ctacgcttcg ttgcccgtca gggcttggag gcggaagccg
     6181 aaagcccgaa agggcattca gcaagggtca gcgcccggaa gggccgggga attcgtttcc
     6241 ggcttccagt cgccgttccg ccgggcgtcc tcccaggggc gtttgtcggg tcggcagttg
     6301 caaccggcgg gtcgcggatt tccgttgcct gccaacccgg agagagcaag tggacagagc
     6361 tggcaagaaa gagctcgtct cgacgctgaa ctcggtgttc aaggacaccg gggccattgt
     6421 cgttgcgcat tattccggcc tcaccgtcgc gcagatgcag aagctccgca aggagatgaa
     6481 ggcagccggc gcttccgtga aggtcgcgaa gaaccgcctt gtgaaaatcg ctctcgaagg
     6541 cacggacgtt gcgtccatcg ggtccttgat gaagggcccg acgatcctgg cttattccaa
     6601 ggatccggtc gcggcgccca aggtcgccgt cgctttcgcc aaggaccacg acaagctcgt
     6661 gatcctgggt ggcgcgatgg gaacgaccgc cctgaaaccc gacggtgtca aggctctggc
     6721 cacgatgccg tcgctggacg aactgcgcgc caagctcgtc ggcctcatcc aggccccggc
     6781 gacgaagatc gcgcagctcc agaccgcgcc ggcagccaag ctggcccgcg tgttcggggc
     6841 ctatgccaag cgagacgagg cggcgtgagc cgtcatcaca ttcatcgaac cgttcgaacc
     6901 gaacatagac gatccaagga actgaaatca tgaccgacct gtcgaaaatc gtcgacgaac
     6961 tgtcgaagct gaccgttctc gaagccgccg agctcgccaa gatgctcgaa gagaagtggg
     7021 gcgtttccgc cgccgccgcc gttgctgttg cggctgctcc gggcggtggc ggtgctgccg
     7081 ctccggtcga ggagcagacc gaattcaccg tgatcctggc ctcggccggc gacaagaaga
     7141 tcgaggtcat caaggaagtc cgcgccatca ccggcctggg cctcaaggaa gccaaggacc
     7201 tggtcgaagg cgc
//
LOCUS       DAOMXN010000132         7209 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_5409_length_7209_cov_10.322617, whole genome shotgun sequence.
ACCESSION   DAOMXN010000132 DAOMXN010000000
VERSION     DAOMXN010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 7209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7209
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5409_length_7209_cov_10.322617"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            587..976
                     /locus_tag="PK812_09545"
     CDS             587..976
                     /locus_tag="PK812_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HRJ69841.1"
                     /translation="MGLAHRKLKVMALTEAAAARIRDQIARSDRPVAGLRVGVKNGGC
                     AGMSYTMDFADEIKPFDEVIEDHGVKLVIDAKAVLFLLGTTLDVKADKFSSGFVFLNP
                     NETSACGCGESVAITPANAEAAADAAR"
     gene            1051..2679
                     /locus_tag="PK812_09550"
     CDS             1051..2679
                     /locus_tag="PK812_09550"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HRJ69842.1"
                     /translation="MARYVFITGGVVSSLGKGLASAALGALLQARGYSVRLRKLDPYL
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTGVSASKQDNITTGRIYQELIAKE
                     RRGDFLGATVQVIPHVTDAIKDFVLTGNEGFDFVLVEIGGTVGDIEGLPFFEAIRQLG
                     NDLPKGQAVYVHLTLLPYIPTAGELKTKPTQHSVQELRSIGIQPDILLCRCDRPIPEG
                     ERRKLALFCNVRPGAVVEAQDARHLYDVPLDYHRAGLDAEILAAFGITDAPAPNLSRW
                     ERISETIRNPEGQVTIAIVGKYTGLKDAYKSLIEALAHGGIANRVKVNLDWIDSEVFE
                     SEDPAPFLEHVSGILVPGGFGQRGTEGKIAAATFARKRNVPYFGICFGMQMATIEAAR
                     NLCGIAAASSTEFGPCGEPVVGLMTEWLSGNELQKRSAEGDLGGTMRLGAYTAALSPG
                     SKVAGIYGSTRISERHRHRYEVNMAYRERLEAKGLRFSGRSPDGLLPEIVELEGHPWF
                     IGVQYHPELKSRPFEPHPLFASFIGAAVERSRLV"
     gene            2881..3300
                     /locus_tag="PK812_09555"
     CDS             2881..3300
                     /locus_tag="PK812_09555"
                     /inference="COORDINATES: protein motif:HMM:NF015927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HRJ69843.1"
                     /translation="MMGHHWFWPSLAGIPAFFIYFAASAAVLTVFGILYIRITAHHET
                     DLIRQGNVAAAVGYGGALIGYSLPLSRSVAQAATIPDFLIWAILAFLVQIGAYWVAKR
                     IVPDISDRISKGDMASAVLLGGLAIACGMLNAASMTL"
     gene            3652..4818
                     /locus_tag="PK812_09560"
     CDS             3652..4818
                     /locus_tag="PK812_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HRJ69844.1"
                     /translation="MKRIGFKERRDWRDKADEAGFTFHSGEDGERYWFEEAAYAFTLK
                     QIEEDLEAPVAELESMCLAFVDKAVKDEAILKKLAIPEFAWPLIAESWTRGDRNLYGR
                     FDLAYDGSGPAKLLEYNGDTPTALFESAVFQWSWLEESIASGALPNGADQFNSVHDRL
                     VAALGQMRRGEKYHLHLACDIESDEDQGTIAYLAECAKQAGMTARCIAMSDIGIADDG
                     QFLDENDEPIRTLFKLYPWEWMLQEEFGQKVGGSQTLFIEPVWKAVLSTKGILPFLWE
                     MAPEHPNLLKAYFADDPRVSELSARHVRKPILSREGANVTVFDGANTTTTDGPYGQEG
                     YIVQDYARPFRQGDTQAIIGAWTVASEPAGICIREQESAVINNMARFVPHYIEP"
     gene            4870..5781
                     /locus_tag="PK812_09565"
     CDS             4870..5781
                     /locus_tag="PK812_09565"
                     /inference="COORDINATES: protein motif:HMM:NF024859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRJ69845.1"
                     /translation="MCADSPAPYSRGARGIDHLVIAGRDLDALAEDYRRLGFTVGTRN
                     RHPWGTENHIVQFPGCFLELIGVGSGAAIQPGQGRSYSFGGFVEHYLRRREGLAMLVL
                     ESRDARADAQAYAEARVGDFEPFFFERQGVKADGTAARVAFTLAFAAMKDAPLAGFFV
                     CQQHEPQNFWNTLAQSHANGVFGISRVSLVADDPSDCHEFLGAFIGQRAMRATSFGLE
                     LETGRGIVEVLSAEGFAFRYGAPPLRTGTTPQFAAAMFRVADCDAAERFLAGAGVAAV
                     RSPGGCLALRDELHGLRLAFEQQGVLP"
     gene            5778..6134
                     /locus_tag="PK812_09570"
     CDS             5778..6134
                     /locus_tag="PK812_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69846.1"
                     /translation="MKFIAIAIIAGVAGFMGWLWFQPECRGGAVVASETACVSITGFD
                     RAFCASAFARPEEAIRRAGGIFPTRDACLKRHTDCIDVAGTGGFGGYAAKPAGFCLVR
                     GADGGLSRMVPVYRGP"
     gene            6153..7013
                     /gene="kdsA"
                     /locus_tag="PK812_09575"
     CDS             6153..7013
                     /gene="kdsA"
                     /locus_tag="PK812_09575"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HRJ69847.1"
                     /translation="MSETNETVVAVGDVRFGNHLPLALIAGPCQMESRAHALETAAFL
                     KDMAARLGIGLVYKSSYDKANRTSLGAARGIGLDKAMDIFAEIRSSLGLPVITDVHET
                     FHCAEVAKTVDVLQIPAFLCRQTDLLVAAAQTGRAINIKKGQFLAPWDMANVVAKVRG
                     AGNSRVMACERGVSFGYNTLVSDMRALPIMAQTCQAPVIFDATHSVQQPGGQGGASGG
                     ERRFVETLARAAVAVGVAGVFIECHEDPDTAPCDGPNMVPLKAMPDLVARLMEFDRLA
                     KKPTSPLQTR"
     gene            7021..>7209
                     /locus_tag="PK812_09580"
     CDS             7021..>7209
                     /locus_tag="PK812_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69848.1"
                     /translation="MPFLAALAGAIMTGVMYWLLFGNGREVISQWLDQRNEQARRDQD
                     TAASMRARISEARAPLRAL"
BASE COUNT         1206 a         2312 c         2409 g         1282 t
ORIGIN      
        1 ccctgcgccg cgcgccgcag cccgaacggc aggaccggcg ccggtccgag ccgcgcgaac
       61 acacgcctct gggcctcggt gatcacgtgc cgagcttcct gatgcggccg gcccgcacga
      121 agtgacgacg ccgcggcacc gtgctttcgt caggagtcag gcccgaccag ttgaacacgc
      181 ttcgttttgg gtgcaacctg gctgcgggga tcacgggaga aaccgccatg aagtgtcgcc
      241 ttgcgcttgt cgcttgcaca gccttgttcc tctgttcgat gccggcctcg gcaagtttgc
      301 agggggcgtc gcagccaaca gggacgccgc gtgaggcggt cggcgcggct ccggtcctca
      361 ccgcgcagta cggcgttcgc aggcgcgcgg tcctgccgca aggcaaggtc aagagccggc
      421 ctgcggcgcg ggtgccggtg ggcaaccgca gaattctgat ccgataaaca ccgccatccc
      481 ggaatgacgc cgccgggaag ccctcgacgg atgcgtgcgc acgcactata ttcatgaaat
      541 ctaatctcag gagccggacc ttgaccccgg cgatccggag gggaccatgg gccttgcgca
      601 ccgcaagctg aaagtgatgg cgctgaccga agcggcggcg gcgcgaatcc gcgaccagat
      661 cgcccgcagc gaccggccgg tggccggcct gagggtcggc gtgaagaacg gcggttgcgc
      721 cggcatgtcc tacaccatgg atttcgcgga cgagatcaag ccgttcgacg aggtgatcga
      781 ggatcacggc gtcaagctgg tcatcgacgc gaaggcggtg ctgttcctgc tcggcacgac
      841 cctggatgtg aaggcggaca agttctcgtc gggcttcgtc ttcctcaatc cgaacgagac
      901 ttcggcctgc ggctgcgggg aatccgtcgc catcacgccg gccaatgccg aggcggccgc
      961 cgacgctgcc cgctaggcct gcctctgtta tccacacctg ccagattttc cttggccgaa
     1021 tcgggggcgg ggggatacac gtcggctccc atggctcggt acgttttcat aaccggtggc
     1081 gtggtttcct ctctcggaaa aggtctcgcc tccgcagcgc tcggcgcgct tctgcaggcg
     1141 cgcggctatt ccgtccgtct gcggaagctc gacccctatc tcaatgtcga tccgggcacg
     1201 atgagcccgt atcagcacgg cgaagtcttt gtcaccgacg atggggcgga gacggacctc
     1261 gatctggggc attacgagcg cttcaccggc gtttcggcgt ccaagcagga caacatcacc
     1321 accggccgca tctatcagga gctgatcgcc aaggagcggc gcggcgactt cctgggtgca
     1381 acggtgcagg tgatcccgca tgtcaccgac gcgatcaagg atttcgtgct caccggcaac
     1441 gaggggttcg atttcgtcct tgtcgagatc ggcggcacgg tgggcgacat cgagggcctg
     1501 ccgttcttcg aggcgatccg ccagctcggc aacgacctgc ccaagggcca ggccgtctat
     1561 gtccacctga cgctgctgcc ctacatcccg acagcgggcg aactgaagac caagccgacg
     1621 cagcattccg tgcaggagtt gcgctcgatc ggcatccagc cggacatcct gctctgccgc
     1681 tgcgaccggc cgattccgga aggcgaacgg cgcaagctcg ccctgttctg caatgtgcgg
     1741 ccgggggccg tggtcgaggc gcaggacgcg cgccatctct atgacgtgcc gctggactac
     1801 caccgtgccg ggctggacgc ggaaattctc gcggccttcg gcatcaccga tgcgccggcg
     1861 ccgaacctgt cgcgctggga gcgcatcagc gagaccatcc ggaacccgga aggtcaggtg
     1921 accatcgcca ttgtcggcaa gtacaccggc ctgaaggacg cctacaagtc gctgatcgag
     1981 gccctggcgc atggcggcat cgccaaccgg gtcaaggtca acctggactg gatcgattcg
     2041 gaagtgttcg aatcggaaga tcccgcgcct ttcctcgaac atgtcagcgg catcctggtg
     2101 ccgggtggct tcggtcagcg cgggacggaa ggcaaaatcg cggcggcgac cttcgcgcgc
     2161 aagcgcaacg tgccctattt cggcatctgt ttcggcatgc agatggcgac gatcgaggcg
     2221 gcgcgcaacc tgtgcggcat cgcggcggca agctcgactg agttcggccc ctgcggcgag
     2281 cctgtcgtcg gcctgatgac ggagtggctg tcgggcaatg agttgcagaa gcggtcggcc
     2341 gagggcgacc tcggcggtac catgcggctc ggcgcctata cggcggcgct gtcgccaggt
     2401 tcgaaagtgg cgggcatcta cgggtcgacg cgcatttcgg agcgccaccg ccatcgctac
     2461 gaggtcaaca tggcctatcg cgagcggctg gaggccaagg gcctgcggtt ttcgggccgg
     2521 tcgcccgacg gactgctgcc cgagatcgtc gaactcgaag ggcatccgtg gttcatcggc
     2581 gtgcagtacc atccggaact gaaatcccgc ccctttgaac cgcatccgct cttcgcgagc
     2641 ttcatcggtg cggcggtgga gcgcagccgg ctggtgtgat ggcatcagcc ctggccactc
     2701 gtgcggcgca ggctggcggt gtcattgccg gggcgccagg cggaacgcgg acttcatgtt
     2761 tctgaaagtc agatgctgag accgattccg ggcacgctcc ttccggatcg cggaggggct
     2821 tcctgcttcc cgcgattcat gcatagttgg cgcgggggct agttctggag cttgaacgac
     2881 atgatgggcc atcactggtt ctggccatcg ctggccggca taccggcgtt cttcatctat
     2941 ttcgcggcga gcgcggcggt gctgaccgtg ttcggcatcc tctacatccg catcaccgcc
     3001 catcatgaga ccgatctgat ccggcagggc aacgtggccg cggcggtcgg ctatggcggc
     3061 gcgctgatcg gctattcgct gccgctctcg cgttcggtgg cgcaggccgc gaccatccct
     3121 gacttcctga tctgggcgat cctcgccttt ctggtgcaga tcggcgccta ttgggtggcg
     3181 aagcgcatcg ttcctgacat ttccgaccgg atttccaaag gcgacatggc ctcggcagtg
     3241 ctgcttggcg gtctcgccat cgcctgcgga atgctcaatg ccgcctccat gacgctctga
     3301 gggcgtcgcc atgaaacgct cgacgatgct tctggtcggc gcggccggca tcacgctggc
     3361 ggcgtggggc acggagcgcg ccatcaatcg accgcgcatg cccgtgccgc tgccgtccga
     3421 gtgcgtgctg gggcctgacg gcaagatgcc agccctgccg caatgccaga ccgctgcggc
     3481 gcagaggtcg gcgtcatcct cctcgaccac cagccgatcc agcagtggct ggacccgaag
     3541 ctacagcggc agcaattcat cggcgtcctc gtcgtccggg tcgcatggac cggcggtgtc
     3601 gcgcggcggc ttcggctcca gttcgcgcag cttttcctcc gggtcatgac catgaagcgc
     3661 atcgggttca aggagcgtcg ggactggcgc gacaaggccg acgaagccgg gttcaccttc
     3721 cactcgggag aagatggcga gcgctactgg ttcgaggaag cggcctatgc ctttacgctg
     3781 aaacagatcg aggaggatct ggaggccccg gtcgcggaac tcgaatccat gtgcctggcc
     3841 ttcgtggaca aggcggtgaa ggacgaagcg atcctgaaga agctcgccat tcccgagttc
     3901 gcctggccgc tgattgccga aagctggacg cggggcgacc ggaacctcta cggccgcttc
     3961 gacctggcct atgacgggtc gggtccggcc aagctcctgg aatacaacgg agacacgccg
     4021 accgcgctgt tcgaatccgc agtgttccag tggagctggc tggaggagtc catcgcctcg
     4081 ggcgcgctgc cgaatggggc ggatcagttc aacagcgtgc acgatcggct ggttgcggcg
     4141 ctgggacaga tgcgtcgcgg cgagaagtac cacctgcatc tggcctgcga catcgagagc
     4201 gacgaggacc agggcaccat cgcctatctg gcggagtgcg ccaagcaggc cggcatgacg
     4261 gcgcggtgca tcgccatgtc cgatatcggc atcgccgacg atggccagtt tctcgacgag
     4321 aacgacgagc cgatccggac gctgttcaag ctctacccat gggaatggat gcttcaggag
     4381 gagttcggcc agaaggtcgg cggatcgcag acactgttca tcgagccggt ctggaaggct
     4441 gtcctgtcca ccaagggcat cctgccgttt ctctgggaaa tggcgccgga acatcccaat
     4501 ctgctgaaag cctatttcgc ggatgatccg cgcgtgtcgg aactgtccgc acgccatgta
     4561 cgcaagccga tcctgtcgcg cgaaggcgcc aacgtcaccg tgttcgacgg cgccaacacg
     4621 acgacgaccg acgggcccta cggccaggaa ggctacatcg tgcaggacta tgccaggccc
     4681 ttccgccagg gcgacacgca agcgatcatc ggcgcctgga ccgtcgccag cgagcccgcc
     4741 ggcatctgca ttcgcgaaca ggagagcgcg gtgatcaaca acatggcccg cttcgtgccg
     4801 cactatatcg aaccgtgacg tccggctcag ggtgacgctg cgacagggcg atgctagtca
     4861 atccgtgcca tgtgtgcaga ttctcccgcc ccctacagcc gtggcgcgcg cggcattgac
     4921 catctcgtca ttgccggacg cgaccttgat gctttggccg aagactatcg gcggctggga
     4981 ttcactgtcg ggacacgcaa ccggcacccc tggggcacgg aaaatcatat cgtccagttt
     5041 ccgggctgtt tcctcgaact gatcggcgtc ggcagcgggg cggcgatcca gccggggcag
     5101 ggacgctcct acagtttcgg cgggtttgtc gaacactatc ttcgccggcg cgaagggctt
     5161 gccatgctcg tgctggaatc acgggatgcg cgcgccgacg cgcaggccta tgccgaggcg
     5221 cgtgtcggcg atttcgaacc gttcttcttc gaacggcagg gcgtgaaggc cgacgggact
     5281 gcggcgcgcg ttgcctttac cctggcgttc gccgccatga aggatgcgcc gctggccggg
     5341 ttcttcgtct gccagcagca cgagccgcag aacttctgga atacgctcgc gcagagccac
     5401 gccaacggcg tgttcggcat ttcgcgcgta tcactggtgg cggacgatcc atccgactgc
     5461 cacgagttcc ttggcgcatt catcggacag cgcgccatgc gggcgacgtc gttcggattg
     5521 gaactggaga ccgggcgcgg catcgtcgag gtgctgtccg cggaagggtt tgctttccgc
     5581 tacggcgcgc cgccgttgag gacggggacg acaccgcaat tcgcggcggc gatgttccgc
     5641 gttgccgatt gcgacgccgc agaacgcttt ctggcaggcg ctggcgtggc ggcggtccga
     5701 tcaccggggg gatgtctcgc cctgagggat gagctccatg gccttcggct ggctttcgag
     5761 cagcaaggcg ttctgccatg aaattcattg caatcgccat catcgcgggg gtcgcgggct
     5821 tcatgggctg gctctggttt cagccggagt gccggggcgg cgcggtcgtc gcgagtgaga
     5881 ccgcctgcgt ctccatcacg ggcttcgacc gggctttctg tgcctctgcc ttcgcgcggc
     5941 cggaggaggc gatccgccgc gccggcggaa ttttcccgac acgggatgcc tgcctcaagc
     6001 ggcatacgga ctgcatcgat gtggccggaa ccggcggttt cggcggctat gccgccaaac
     6061 ctgccggatt ttgtctggtg cgcggcgcgg atggcgggtt gtcacgcatg gttccggtct
     6121 atagaggccc ctgacgcatc caggggtagc cgatgagcga aaccaatgag accgttgtcg
     6181 cggtgggtga tgtccgcttc ggcaaccatc tgccgctggc gctgatcgcc ggcccgtgtc
     6241 agatggagag ccgcgcccat gcgctcgaaa cggcggcctt cctcaaggac atggccgcca
     6301 ggctcggcat cgggctggtc tacaaatcct cctatgacaa ggccaaccgg acgagcctcg
     6361 gcgccgcgcg cggcatcggc ctcgacaagg ccatggacat cttcgccgaa atccggagca
     6421 gtctcggctt gccagtgatc accgacgtgc acgagacctt ccactgcgct gaagtggcga
     6481 agactgtcga tgtcctgcaa attccggcct ttctctgccg gcagaccgac ctgctggtgg
     6541 ccgctgccca gactggcagg gcaatcaaca tcaagaaggg ccagtttctc gccccctggg
     6601 acatggccaa cgtcgtggcc aaggtgcggg gcgccggcaa ctcgcgcgtg atggcctgcg
     6661 agcgcggcgt ctccttcggc tacaacaccc ttgtctcgga catgcgcgct ctgccgatca
     6721 tggcccagac ctgccaggct ccggtcatct tcgatgcgac ccactcggtg cagcagccag
     6781 gtgggcaggg cggcgcctcc ggcggagagc gccggttcgt cgagacgctg gcgcgggcgg
     6841 ccgtggcggt cggcgtcgcc ggcgtattca tcgagtgcca cgaggacccg gacaccgcac
     6901 cctgcgacgg gcccaacatg gttccgctga aagccatgcc ggatctggtt gcgcggctca
     6961 tggaatttga cagactggcg aagaagccaa ccagtcccct tcagacgagg taagtgtcgc
     7021 atgccgtttc tcgccgccct ggccggcgcc atcatgacgg gcgtcatgta ctggcttctg
     7081 ttcggcaatg gacgggaagt catcagtcag tggcttgacc agcgcaacga acaggcgcgt
     7141 cgcgatcagg acacggcggc ctcgatgcgc gcgcgcatca gcgaggcgcg ggcgccgctg
     7201 cgcgcgctg
//
LOCUS       DAOMXN010000133         7207 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_5411_length_7207_cov_11.688898, whole genome shotgun sequence.
ACCESSION   DAOMXN010000133 DAOMXN010000000
VERSION     DAOMXN010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 7207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7207
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5411_length_7207_cov_11.688898"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            360..713
                     /locus_tag="PK812_09585"
     CDS             360..713
                     /locus_tag="PK812_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004688588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HRJ69849.1"
                     /translation="MSLLLKPHILPIILLVASNVFMTIAWYGHLKFKTSPLWIAVTAS
                     WGIAFVEYWLAVPANRIGHQVYSAAELKTMQEVITLVVFAVFSVTYLGEALSWKHGVG
                     FALIAAGAAFVFQGR"
     gene            759..1529
                     /locus_tag="PK812_09590"
     CDS             759..1529
                     /locus_tag="PK812_09590"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRJ69850.1"
                     /translation="MTAPKIEISSGIPALDAFLDGPYETVKGMSSRFAAAISGHLLRR
                     QSELGIAGDVAEIGAFKGRFFIAMMKALAAGEKGLAIDVFSWPTEACYDEFLANCATH
                     GVRPEDMIVWKTDTRDMSVADIRSKLNDRPVRFFHIDGDHSDVCLAKDLELATAVLHP
                     NGIICLDDMLHPGYPTLVATVMRYLKDHPDMRVVLVVDREDIVAAPKFMLCRADAVAL
                     YEDDLMKSFAPFHYVLGADFVDHWCVVLTPRPRLAIVD"
     gene            complement(1570..1749)
                     /locus_tag="PK812_09595"
     CDS             complement(1570..1749)
                     /locus_tag="PK812_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69851.1"
                     /translation="MCTASRIDRKDLDDFADLRRPCDTTALRIAAGALIFGTLIASTT
                     LYLRAMEAAASILLH"
     gene            complement(1935..2156)
                     /locus_tag="PK812_09600"
     CDS             complement(1935..2156)
                     /locus_tag="PK812_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69852.1"
                     /translation="MKTQDPINLTSFRSEATRQHSRALEQQYRIAIGLVAVLCAATVA
                     AIITLAPMDRTHVGLDMTASGKTVSLGRS"
     gene            2387..4177
                     /locus_tag="PK812_09605"
     CDS             2387..4177
                     /locus_tag="PK812_09605"
                     /inference="COORDINATES: protein motif:HMM:NF017775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HRJ69853.1"
                     /translation="MAVNDASLARQRDATGGGDATVWHDLKWFVRALKASPSRWRIFG
                     VLGAIVVILIGNMTAQVFLNRWNGQFFDSISAKNLDAFIHYTGMFFVIAGILLALVVA
                     QTLAQELLKIELRRFLTVHIADMWLQRARPYQLAFAGAIGSNPDQRVQEDTRKLAEFT
                     AELATGLIQQTFLLTTFVGVLWNLSSALVFRIGGADVTIPGYMVWCAMLYAFLGSALT
                     FLVGKPLIVLNAERSQKEAELRFALVRLSESAESIGFLRGEGDERRGLNAPIGAVLGA
                     MRAIAYKLARLTWVTSGYGWLSLIVPVIVAAPAYFTSSVTFGGLMRLVDAFGQVQASL
                     RWFVDNFAKIADWRAALFRVMRFEEELRRSEIEEQDREGIRIETGEDGALRLDDLRVA
                     LPGGAAIIAHAALMLAPGERAQLSGPPGSGKSTLLRAIAGLWPWGAGSVTTPPEGTMM
                     FLPPRPYLPLGTLREAVCYPGKPEAFGDGEVRSALRRCGLESWIDQLDVAARWDRDLT
                     TGDQQRLTFARLMLHRPALIFMDEATSALDEIGQADLFRLFDEELANAAILLVSHRGG
                     FEAFASRRIVLDTGSEGSVLKEVRPASSNR"
     gene            complement(4152..4454)
                     /locus_tag="PK812_09610"
     CDS             complement(4152..4454)
                     /locus_tag="PK812_09610"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HRJ69854.1"
                     /translation="MVIVIARITCQPGKRAALLDAARAAIVETRKEPGCISYSAYADS
                     MDENLAIFYEEYASEAACMEHMGRPYTQALIAAAPQFVAAAPSIIMHGVSGSRTLA"
     gene            complement(4556..5536)
                     /locus_tag="PK812_09615"
     CDS             complement(4556..5536)
                     /locus_tag="PK812_09615"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRJ69855.1"
                     /translation="MILRQTRRQMLAAGAALVVAPGGARAQGFPSKPVKIVVPYPPGG
                     PTDGLARIVAEDIRADLGQNVIVENMAGASGAIGTRAVARAEADGHTIVFGTNQTHCT
                     NAFLLKEPGYDPVKDFAPLAALADLQHVLVLKKDLAAATGPDLAALARKEPGKLNYGS
                     TGLGSGSHLAMELFRARTGTEMQHIPFPGAAPMAQEIMAGRIDAAMATLPSVLGMIQG
                     GQMKAAALASATPAPQLADVKLLRDQGITDAEADAWLAFFAPAAIPAAVRDRLAGAIL
                     ASMRKPEVKERATKLGFAVNLKDSGQLAAFQATEMKKWTAVAQAAGLKAQ"
     gene            5658..6860
                     /locus_tag="PK812_09620"
     CDS             5658..6860
                     /locus_tag="PK812_09620"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRJ69856.1"
                     /translation="MAFLADVLSKVKPSATIALTQAARDLRAKGKDVISLSVGEPDFD
                     TPDNIKKAAIDAINRGETKYTPVAGIQPLREAIAAKFKRENGLDYKWQQTIVGTGGKQ
                     VLFNAFMATLNPGDEVLIPAPYWVSYPEMVALCGGTPVPVMTSIGNGFKVTAEALEKA
                     ITPKTKWFLFNSPSNPSGAAYTRAELKALTDVLKKHRHVWTLTDDMYEHLVFGDFVFT
                     TPAQVEPELMDRTLTMNGVSKAYAMTGWRIGYAAGPDQLIKAMDMVQGQQTSGACSIA
                     QWAAVEALNGPQDFITTSKKAFEERRNLVVSMLNQTRGLKCPMPEGAFYVYPSCAELI
                     GKKAPSGKIITTDEDFVMELLNTEGVAAVHGSSFGLGPNFRVSYATSNDKLVDACQRI
                     QRFCAELR"
     gene            6891..>7207
                     /locus_tag="PK812_09625"
     CDS             6891..>7207
                     /locus_tag="PK812_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69857.1"
                     /translation="MRRALKGTACAALMVPAGLCGVAAEDLVRPFAPVETATVQRSVS
                     GSIFGLSSASSTVADKAVSPGLPLDITPSQAALTAAAAALAAAPKPANVPLPPRVERA
                     GAP"
BASE COUNT         1185 a         2248 c         2436 g         1338 t
ORIGIN      
        1 tccttgcagg aagtggaggc ggccggcgcg acgaggctag gctttttcgc gccgagatcg
       61 gcggcggaag cgccggtcat gaggaggccg gaaagcccgg cggcaagagc gaaacggatc
      121 atctgaaagg tccttgttgt tgagacaacc aggacgattg ttttttccga tgctctctta
      181 agacaatgat tttgtttgtt ttttctgctc agtttctaag caatctgcct ttgtgcggtg
      241 cacaaaatcc atatggttgc ggtgtgttgc gtcgctgcaa ctgcgcgtcc tgcaggtcct
      301 ctcgccggac atgcccaatg atgcgatgat gacaggacgc aaccaatccg gatggcgtca
      361 tgtcccttct gctgaaaccc cacatcctgc cgatcattct gctggtcgcc tcgaacgtct
      421 tcatgacaat cgcctggtac ggccatctca agttcaagac ctcgcccctg tggatcgcgg
      481 tgacggcaag ctggggcatc gccttcgtcg aatattggct ggcggtgccg gccaaccgga
      541 tcggccatca ggtctactcg gcggcagagc tgaaaacgat gcaggaggtg atcacgcttg
      601 tcgtcttcgc ggtgttttcc gtgacctatc tgggcgaagc gttgagctgg aagcacgggg
      661 tcgggttcgc gctcattgcg gccggggcgg ctttcgtgtt tcaagggcgc tgatgcgcgg
      721 cgatgccgcg cggatcagtg acaggctgga cagcacccat gacggcgccg aaaatcgaga
      781 tttcctcagg cattcccgcg ctcgacgcct tcctcgacgg gccctatgaa accgtaaagg
      841 gcatgtcgtc gcgcttcgcg gcggcgatca gcgggcacct cctgcgccgg cagagcgaac
      901 tcggcatcgc tggcgatgtg gccgagatcg gcgccttcaa ggggcgtttc ttcatcgcca
      961 tgatgaaagc gctggcggcg ggcgagaagg gccttgcgat cgacgtgttc tcatggccga
     1021 cggaggcctg ttacgacgaa ttcctcgcca actgcgcgac acacggcgtc aggcccgagg
     1081 acatgatcgt ctggaagacc gatacgcggg acatgtcggt cgccgatatc cggtcgaagc
     1141 tgaacgaccg gccggtgcgc ttcttccata tcgacggcga ccattccgat gtctgcctgg
     1201 ccaaggatct ggaactggcc acggcggtct tgcatcccaa cggcatcatc tgcctcgacg
     1261 acatgctgca cccgggctat ccgacgctgg tggccacggt gatgcgctac ctgaaggacc
     1321 atcccgacat gcgggttgtt ctcgtggtgg accgggagga cattgtcgcc gcgcccaaat
     1381 tcatgctctg ccgggccgat gccgtggcgc tctatgaaga cgatcttatg aagagcttcg
     1441 cgccgttcca ctatgtgctg ggcgccgatt tcgtcgatca ctggtgcgtc gtgctgacgc
     1501 cccgtccgcg gctggcgatc gtcgactgaa ccgccgggcc ggtgcacccc gccgggtgcg
     1561 acgggcgcgt cagtgcagca ggatcgaggc ggccgcttcc atcgccctga gatagagcgt
     1621 cgtcgaggcg atcagcgtgc cgaagatcag cgccccggct gcgatccgca gggcggtggt
     1681 gtcgcatggg cggcgaagat cggcgaagtc gtcaaggtcc ttccggtcga tccttgaggc
     1741 ggtacacatg attcactcct gtccttgtca gtcggatgcc gactgcgtgt tgttcctcca
     1801 gttaaccatg attgtgccct tccgatgtgc gctggcgcac atctgacgcg tccgtgaact
     1861 caggtgatcc ggcgtgaacg caaaaaaaga cccgcgccaa agcgcgggcc atgtcttcga
     1921 atgggtggat cggatcagct gcggccaagg ctcacggtct tgccgctggc cgtcatgtcc
     1981 agaccaacat gtgtcctgtc catcggcgcc agagtgatga tggctgcaac ggtggcggcg
     2041 cacagcacgg cgacgaggcc gatcgcgatg cggtactgct gctcgagcgc acgcgaatgc
     2101 tggcgggtgg cttcgctgcg gaagctggtg aggttgatcg ggtcctgcgt cttcatggcg
     2161 gtcatccctg tcgaggcccg gtttcgtcca cctcggtccg catgaccgct gcgtcgaaac
     2221 cgttgtcgcc ctgcaccctt ccttaagccg ccgcccaacg gaggaagtgc gctggcgcac
     2281 atggccgacg ttaaccatac cggctgaagc aggccgcaat ctggcgttca tgcggcattt
     2341 atccgatatg ggggaaagga ccgggtgtcc gggacgggag aaactcgtgg cggtgaacga
     2401 tgcgtccctg gccaggcagc gcgatgcgac aggcggcggc gacgcgacgg tgtggcacga
     2461 tctcaaatgg ttcgtgcggg cgctgaaggc ttcgccctcg cgctggcgga tcttcggcgt
     2521 gctcggcgcc atcgtcgtca tcctcatcgg caacatgacg gcgcaggtgt tcctcaatcg
     2581 ctggaacggg cagttcttcg attcgatcag cgccaagaac ctcgacgcct tcatccacta
     2641 caccggcatg ttcttcgtca tcgccggtat cctgctggcg ctggtcgtgg cgcagacgct
     2701 ggcgcaggaa ctgctgaaga tcgaactgcg acggttcctg accgtgcata tcgcagacat
     2761 gtggttgcag cgcgcccgcc cctaccagct tgcttttgcc ggcgcgatcg gcagcaatcc
     2821 ggaccagcgc gtgcaggaag atacgcgaaa gctggccgaa ttcaccgcgg aactggcgac
     2881 cggcctgatc cagcagacct tcctgctgac gacgttcgtg ggcgtcctgt ggaacctgtc
     2941 ctcggcgctt gtgttccgca tcggcggcgc tgacgtcacc attccgggtt acatggtgtg
     3001 gtgtgccatg ctctatgctt tcctgggctc ggcgctgacg ttccttgtcg gtaagccgtt
     3061 gatcgtgctg aatgcggagc gctcgcagaa ggaagcggaa ctgcgctttg cgctcgtgcg
     3121 cctgagcgaa agcgcggaga gcatcggctt cctgcgcggg gaaggcgacg aacgacgcgg
     3181 cctgaatgcg cccatcggcg ccgtgctggg tgccatgcgc gccatcgcct acaagctggc
     3241 gcggctcacc tgggtcacgt cgggttacgg ctggctgtcg ctgatcgtgc ccgtgatcgt
     3301 ggcggcgccg gcctatttca ccagttccgt gaccttcggc ggcctgatgc gactggtcga
     3361 cgccttcggc caggtccagg cttcgctgcg ctggtttgtc gacaacttcg ccaagatcgc
     3421 ggattggcgc gccgcgctgt ttcgcgtcat gcgtttcgag gaggagctgc ggcgctccga
     3481 gatcgaggag caggaccgcg aaggcatccg catcgagacc ggcgaggatg gcgcgctgcg
     3541 gctggatgac ctgcgggttg ccctgcccgg cggcgcggcg atcatcgccc atgcggcatt
     3601 gatgctggcg ccgggcgaga gggcgcagct ttccgggccg ccgggttcgg ggaagtcgac
     3661 gctgctcagg gcgatcgcgg gattatggcc gtggggcgcg gggtctgtca ccacgccgcc
     3721 ggaagggaca atgatgttcc tgccgccgag gccctacctg ccgctcggca cgctgcgcga
     3781 ggcggtgtgc tatccgggca agccggaggc gttcggcgat ggcgaggtgc gctcggccct
     3841 gcgtcgctgc gggcttgaaa gctggatcga ccagctggac gtcgccgcac gctgggaccg
     3901 cgacctgaca accggggacc agcagcgcct gacctttgcc cggctgatgc tgcaccggcc
     3961 ggcgctgatc ttcatggacg aggcgacatc ggcgctggac gaaatcggtc aggccgacct
     4021 gttccggctg ttcgacgaag aactcgccaa cgccgcgatc ctgttggtgt cgcacagggg
     4081 cgggttcgag gccttcgcca gccggcgcat cgtgctggac accggcagcg agggcagcgt
     4141 gctgaaggag gtcaggccag cgtcctcgaa ccgctgacgc cgtgcatgat gatcgacggt
     4201 gccgcggcga cgaactgcgg cgcggcggcg atcagggcct gggtgtaggg ccggcccatg
     4261 tgttccatgc aggcggcctc ggaggcatat tcctcgtaga agatcgcaag gttctcatcc
     4321 atggaatccg cgtaggcgga gtaggagatg cagcccggct ccttgcgcgt ctcgacgatg
     4381 gcggcgcggg ccgcgtccag cagcgcggcg cgcttgcccg gctggcaggt gatgcgggcg
     4441 atgacgatga ccatgggtcc ctccttcccg gtgacgacag ccagactaca ccgccagggg
     4501 cgccatggac gcatcgccgg tcatgatgtt ttcgtgagca ccagcccgct tcaggtcact
     4561 gcgccttcag cccggccgcc tgtgccacgg cggtccactt cttcatttcc gttgcctgga
     4621 aggcggcgag ttggccggaa tccttgaggt tgaccgcgaa accgagcttc gtggcgcgct
     4681 ccttgacttc cggcttgcgc atggacgcca ggatggcgcc agcaaggcgg tcgcgcacgg
     4741 cggccgggat ggcagcaggc gcgaaaaagg cgagccaggc atccgcctcg gcgtccgtga
     4801 tgccctgatc gcgcaggagc ttcacgtcag ccagttgcgg ggccggggtg gcgctggcca
     4861 gcgccgctgc cttcatctgc ccgccctgga tcatgccgag cacgctcggc agcgtcgcca
     4921 tcgcggcatc gatgcgcccg gccatgatct cctgcgccat cggggccgcg cccgggaagg
     4981 ggatgtgctg catctcggtt ccggtgcggg cccggaacag ttccatcgcc agatgcgagc
     5041 cggagccaag accggtggag ccgtagttga gcttgcccgg ctccttcctg gcgagcgccg
     5101 cgaggtccgg gccggtcgcg gcggcaaggt ccttcttcag taccagcacg tgctggagat
     5161 cggccagcgc cgcgaggggg gcgaaatcct tcaccgggtc gtagcccggc tccttcagga
     5221 ggaaggcatt ggtgcaatgc gtctggttgg tgccgaacac gatggtgtgc ccgtcggcct
     5281 cggcgcgggc gacggcgcgc gtgccgatgg cgccggaagc gccggccatg ttctcgacga
     5341 tcacgttctg gcccagatcg gcgcgaatgt cctcggctac gatgcgggcg aggccgtccg
     5401 tcgggccgcc gggcggatag ggaacgacga tcttcaccgg cttggatggg aagccctgcg
     5461 cgcgggcccc gccgggtgcg acaacgagcg ctgcgccggc tgcgagcatc tggcggcggg
     5521 tttggcgaag gatcatgacg gcgtctctcc cgttaacgtt ttgtgccctc aaagggcaaa
     5581 tcggcttgat gcatccgggc gcttcgccta ggaggtttct gccctccgaa ggggggtgga
     5641 cacccggagt ctcgatcatg gcctttctcg ccgacgttct ttccaaggta aagccctccg
     5701 cgaccatcgc gctgacgcag gctgcgcgcg acctgcgcgc caaaggaaaa gacgtgatct
     5761 cgctctcggt cggtgagccg gatttcgata cgcccgacaa catcaagaag gccgcgatcg
     5821 acgcgatcaa ccggggcgaa acgaagtaca cccccgttgc cggcatccag ccgctgcgcg
     5881 aagcgatcgc cgccaagttc aagcgcgaga acggcctgga ctacaagtgg cagcagacga
     5941 ttgtgggcac cggcggcaag caggtgctgt tcaacgcctt catggcgacg ctgaacccgg
     6001 gtgacgaggt gctgatcccg gcaccctact gggtcagcta tcccgagatg gtggcgcttt
     6061 gtggcggcac gccggttccg gtgatgacct cgatcgggaa cggcttcaag gtgacggccg
     6121 aggcgctcga aaaggcgatc acgccgaaga ccaaatggtt cctgttcaat tcgccgtcca
     6181 acccctcggg cgcggcctat acacgtgccg aactcaaggc gctgacggac gtcctgaaga
     6241 agcacaggca tgtgtggacg ctgaccgacg acatgtacga gcatctcgtt ttcggcgatt
     6301 tcgtcttcac cacgccggct caggtcgaac cggaactgat ggaccggacg ctcaccatga
     6361 acggcgtttc caaggcttac gccatgaccg gctggcgcat tggctacgcc gccgggccgg
     6421 atcagctgat caaggccatg gacatggttc agggacagca gacctccggc gcctgctcca
     6481 tcgcgcaatg ggcggcggtc gaggcgctga acgggccgca ggacttcatc acgacctcga
     6541 agaaggcgtt cgaggaacgg cgcaacctcg tcgtgtcgat gctcaaccag acgcgcgggc
     6601 tgaagtgccc gatgccggag ggtgcgttct acgtctatcc gtcctgcgcc gaactcatcg
     6661 gcaagaaagc cccgtcaggc aagatcatca ccacggatga ggacttcgtg atggaacttc
     6721 tgaacactga gggtgtcgcg gcggtgcatg gctcgtcctt cgggctcgga ccgaacttcc
     6781 gcgtgtccta cgccacgtcg aacgacaagc ttgtcgatgc ctgccagcgc atccagcggt
     6841 tctgtgcgga gctgcggtaa ggcgggcgac gaccggtgag ggccggttcc gtgcgcaggg
     6901 cgctgaaggg cacggcttgc gccgcgctga tggtgcctgc cggcctttgc ggcgtcgccg
     6961 ccgaagatct tgtgcggcca ttcgcgccgg tcgagaccgc cacggtgcag aggtcggttt
     7021 ccggatcgat ctttggcttg tcctcggctt cttcgacagt ggcggataag gccgtctcgc
     7081 cgggcctgcc gctggacatt acaccctcgc aggctgccct gacggccgca gccgccgcgc
     7141 tcgccgccgc tccgaaaccg gcgaatgttc ccttgccgcc gcgcgtcgag cgcgctggag
     7201 cgccgca
//
LOCUS       DAOMXN010000134         7126 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_5493_length_7126_cov_12.424551, whole genome shotgun sequence.
ACCESSION   DAOMXN010000134 DAOMXN010000000
VERSION     DAOMXN010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 7126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7126
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5493_length_7126_cov_12.424551"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="PK812_09630"
     CDS             complement(<1..618)
                     /locus_tag="PK812_09630"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRJ69858.1"
                     /translation="MSVLVTGAGVIGSLTAALLAGAGAKVVLADVRPGEAPAAGIARI
                     VLDVSDFSALERVIAEHGVTRIIHTAAMLSTGIRRSPLDGYRVNTLGAAHVLEAARRH
                     GLGRVVLASSATVGYPVFGSHGPQPIEEDFSMRVHGERPTSIYAATKLANEHLGLIYA
                     DLYGVDCLFLRYGAVIGGPLDAPTSVPGRLVAVLAEAARTGAGRLD"
     gene            complement(615..1907)
                     /locus_tag="PK812_09635"
     CDS             complement(615..1907)
                     /locus_tag="PK812_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRJ69859.1"
                     /translation="MNAGLWALLLSVVVGALLRLPLALVMFSGGATYLFVTKQDIGLL
                     VGQVMNQMTTMYVLVAIPMFILAANIMNAASISDRLWALANALVGRFRGGMGHVTVLV
                     SMIFSSMSGSAVTDAAGPGLVATRMMRDIGKYPAGLACAITAASATIAPVIPPSIPLV
                     IYALLSGASIGALFLAGLVPGMLMGGALMLAIRLIAGRKNLPRGEAVPVREMPAVFGR
                     AALSLTLPVVLLGGIWSGIFTPTEAAAVAAFWALVLGVVVYRRMSVGVTWGVFAESAR
                     QSASTMLLIASAFILNYAIANERLAESLVALISAWDLSPTQFMLVVNVFFLILGCFLD
                     GSVMMLVFVPLLIPSAKLLGIDLTYFGIVVTLNFMIGLITPPYGLLLFVMASLTRVPL
                     IAIIKEIVPFVVTLGGLLLILTLFPAITLFLPRYFGYQ"
     gene            complement(1904..2413)
                     /locus_tag="PK812_09640"
     CDS             complement(1904..2413)
                     /locus_tag="PK812_09640"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HRJ69860.1"
                     /translation="MASGPSGSLRRAAGIIAALSMLAIFVLFVFGVGMRYLANRPQNW
                     VDESVTLLSVWSTFWTAAFVLKWPEHIAFDVVFNHLSAERQRFCLLAGALAFVLLIGA
                     AMPGMVDYTLFLWRERTDALQWRLDYVYSIFPVFFAVVLIRLLLVVKALAGPDWRAEL
                     ARWAGEATP"
     gene            complement(2424..3410)
                     /locus_tag="PK812_09645"
     CDS             complement(2424..3410)
                     /locus_tag="PK812_09645"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRJ69861.1"
                     /translation="MKPIRLAALALAAGILAAPPVSAQTEIRISTAAPTSAPLTAAFN
                     RFKATLEAKLPGQLKVSVHPASALFRQGTEVPGMQRGNLEMASPVVPEIEAQLPEWGA
                     LGGAYVFRDADHMLKTFRGEIGQEFVKDVADKMGIILLEPLYLGTRTVNLRTDKVVKL
                     PSDLAGVKMRMPPGPGFQAIAKALGATAVSMPITEVYLGLKTGAIDAQDNPTNMTRDW
                     KFEEVTAQIIMTRHLVQPTFIAIAKPFFDKLPADQQAAIRAAAKEAAEAQIKGSLQDE
                     IDALEGFKKGGKMKIVEPDIAAFRAAVQAEYKATGLMDKWKPGLLARIDAVK"
     gene            complement(3460..4362)
                     /locus_tag="PK812_09650"
     CDS             complement(3460..4362)
                     /locus_tag="PK812_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRJ69862.1"
                     /translation="MAQWALATVEVEGRAVACMEAGGALYRLQPALERVGYAGGSSIA
                     ALFADWERALAALNAAVARLHKADAVMPSRRLAPLLYPGKILCAGANYFDHLAEMGMP
                     GAKKADQRLFFFMKPPRNAVVGEGATVHMPIGTKAFDWEIELGCIIGKRARNVPVEKA
                     LEHVAAYTVTIDFSARDHNRAPETFYKLDWVAGKANDTCCPMGPRLVPASAIANPQDI
                     GLKLWVNGELKQDGRSTDMIFSIAEQISTASRIMTLDPGDLLLTGTPAGVGVPKQTFL
                     KVGDAVDAEIEGIGRLSVVIQPER"
     gene            4445..5134
                     /locus_tag="PK812_09655"
     CDS             4445..5134
                     /locus_tag="PK812_09655"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HRJ69863.1"
                     /translation="MSIADGRSQAERAYKVLRGEILHGDLMPGERLRAADLQDRYNLG
                     LTPIREALMRLSSENLVEAETNRGSRVTETSAQELADLMATRRAIERLCLTASIERGD
                     ALWEANIVAAMHLLSRTPLPVSASDREAASRWEVQHRSFHFALVAACGSPWLLRFWNT
                     LADHSERYRKLRLLHLHEIRDTVRDINTEHAEIMASVLARDVARSTALMDDHLAATEK
                     WVARLLETQGG"
     gene            complement(5153..6616)
                     /locus_tag="PK812_09660"
     CDS             complement(5153..6616)
                     /locus_tag="PK812_09660"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017487428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HRJ69864.1"
                     /translation="MVWDDFKRVETPEAAVDELVFLHTQATGALRASLERYLKKKVPP
                     QPNERARFRYPELRLSYKPQGVLPSNRRAYAKFHRPGVFSSTITQPGDFREYLLEQLR
                     PLVAEYGATIEVGLSNQEIPFPYVVDKGDEMGRSGVTAAELARFFPVPQLSAVGDEIA
                     DGTFHLANAGPWPLALFDAARTDYSLKRLMHYTGSHWKHVQPFILLTNYHRYVDQFVR
                     WGMTQIGGKSHYTKLVLPGNVEVTTPKSGKELESLLDIAAWSKFQMPAYHLAGAGGGI
                     TLVNIGVGPSNAKNITDHLAVLRPHCFLMVGHCGGLRQSQEIGDYVLAHAYLRQDRIL
                     DALVPPDIPIPALAEVQVALQEAAGMVTGERGDKLKARLRTGTVVTYDDRNWELRWSQ
                     ERQRINLSRAIAVDMESGTIAANGYRLRVPYGTLLCVSDKPLHGEIKLPGAASAFYER
                     AVGQHLQIGLTAVDLLRGKLDQLHTRKLRSFDEPAFR"
     gene            6728..>7126
                     /locus_tag="PK812_09665"
     CDS             6728..>7126
                     /locus_tag="PK812_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HRJ69865.1"
                     /translation="MDSLPRKRRSLVLLVALHLTALVILLLTETALVPKLAFLVSWLF
                     FNFGWMVLLRRPAMAAALALFFLCLLIVLSKLKHEILLMTVNFVDILLIDPDTFAFLM
                     QVFPGLGLRSAGAALLIIGALAIVWRNDVLR"
BASE COUNT         1246 a         2417 c         2284 g         1179 t
ORIGIN      
        1 gtccagccgg cccgctcccg tccgcgccgc ttcggccagc accgccacca gtcggcctgg
       61 cacgcttgtc ggcgcgtcga gcggcccgcc gatcaccgcg ccgtagcgca ggaacaggca
      121 gtcgacgccg tagagatcgg catagatcag cccgagatgc tcgttggcga gcttggtggc
      181 ggcgtagatg ctggtcggcc gctcgccatg cacccgcatg gagaaatctt cctcgatcgg
      241 ctgcggacca tggctgccaa acacgggata gcccaccgtc gccgaactcg cgagcacaac
      301 ccgtcccagc ccgtgccgcc gtgccgcttc cagcacatgc gccgcgccca gggtattgac
      361 gcggtagccg tcgagcgggc tccgccggat gccggtggag agcatcgccg cagtatggat
      421 gatgcgcgtc acgccatgct cggcaatcac ccgctccagc gcggaaaagt cggacacgtc
      481 cagcacgatc cgcgcaatcc cggcagccgg tgcttcgccg gggcgcacgt cggccagaac
      541 gacctttgcg cccgcgcccg ccagcagggc ggccgtcaga ctgccgatga cgccggctcc
      601 ggtgacgagg acgctcattg gtaaccgaag tatcggggca ggaagagcgt gatcgccggg
      661 aacagcgtca ggatcagcag cagacctccg agcgtgacca cgaacggaac gatctccttg
      721 atgatcgcga tcagcggcac gcgcgtcagc gaggccatga cgaacagcag cagcccgtaa
      781 ggcggcgtga tcagcccgat catgaaattg agagtcacca cgatgccgaa atacgtcagg
      841 tcgatgccga gcagtttcgc gctcgggatc agcagcggca cgaacaccag catcatcacc
      901 gatccgtcga ggaagcaccc gaggatcagg aagaagacgt tgaccaccag catgaactgc
      961 gtcggcgaga gatcccaggc cgagatcagc gccacgaggc tttccgccaa ccgttcattg
     1021 gcgatggcat agttcaggat gaaggcgctg gcgatgagca acatcgtcga ggcggactgg
     1081 cgcgcgcttt cggcgaagac gccccaggtg acgccaacac tcatgcggcg atagaccacc
     1141 acgccaagga caagcgccca gaacgcggcg accgccgccg cttccgtcgg cgtgaaaatc
     1201 ccggaccaga tgccgccgag caggaccacc ggcagcgtca gggacagtgc cgcgcgcccg
     1261 aacaccgccg gcatctcgcg caccggcacc gcttcgccgc gcggcaggtt cttgcgcccg
     1321 gcgatcaagc ggatcgccag catcagcgcc ccgcccatta gcatgccggg aacgaggccg
     1381 gccaggaaca gcgcgccgat ggaggcgccg gacagaagcg cgtagatcac cagcgggatc
     1441 gacgggggga tcaccggcgc gatcgttgcc gaggccgccg tgatcgcgca ggcgagcccc
     1501 gcaggatact tgccgatgtc gcgcatcatc cgcgtggcca ccagccccgg cccggccgca
     1561 tccgtcaccg cgctgccgct catggaggag aaaatcatcg agaccagaac ggtcacatgg
     1621 cccatgccgc cgcggaaacg accgaccagc gcgttcgcca gcgcccaaag ccggtcgctg
     1681 atcgatgcgg cgttcatgat gttggcggcc aggatgaaca tcggaatggc gacgagaaca
     1741 tacatcgtcg tcatctggtt catgacctgc ccgaccagca ggccgatgtc ctgcttggtg
     1801 acgaacagat aggtggcgcc gcccgaaaac atcaccagcg ccagcggcaa ccgcagcagc
     1861 gcgccgacga cgaccgacag cagcagcgcc cacaggccgg cgttcatggc gtcgcctccc
     1921 cggcccagcg ggccagttcg gcgcgccagt caggtccggc cagcgccttg acgacgagca
     1981 acaatcggat cagcacgacg gcgaagaaca ccggaaagat cgaatagaca tagtcgagcc
     2041 gccattgcag cgcgtccgtg cgctcccgcc acaggaacag cgtgtaatcg accatgcccg
     2101 gcatcgccgc gccgatcagc aggacgaagg caagcgcccc ggccagcagg cagaagcgct
     2161 gacgctcggc gctgaggtga ttgaagacaa cgtcgaaggc aatgtgttcc ggccacttca
     2221 ggacgaaggc cgccgtccag aacgtcgacc agacgctgag cagcgtcacc gattcgtcga
     2281 cccagttctg cggccggttg gcgaggtagc gcatgccgac gccgaagacg aacagcacga
     2341 agatcgccag catgctcagg gcggcgatga tgcccgccgc cctgcgcagg gatccagaag
     2401 gcccggatgc catcgcgtgc cctttacttc accgcgtcga tgcgggcgag caggccgggc
     2461 ttccacttgt ccatcagacc ggtggccttg tattcggcct gcacggcggc gcggaacgcg
     2521 gcgatgtccg gctcgacgat cttcatcttg ccgcccttct tgaagccttc gagcgcgtcg
     2581 atctcatcct gcagcgaccc cttgatctgg gcctccgccg cttccttcgc ggccgcgcgg
     2641 atcgccgcct gctggtcggc cggcagcttg tcgaagaacg gcttggcgat ggcgatgaag
     2701 gtcggctgca cgaggtggcg cgtcatgatg atctgcgccg tgacttcctc gaacttccag
     2761 tcgcgcgtca tgttggtcgg gttgtcctgc gcgtcgatcg caccggtctt caggccgaga
     2821 tagacctcgg tgatcggcat cgacaccgcc gtcgcgccca gcgccttggc aatcgcctgg
     2881 aaacccggcc ccggcggcat gcgcatcttc acgccagcga ggtcggaagg aagcttcacg
     2941 accttgtcgg tgcgcagatt gaccgtccgc gtgccgaggt agagcggctc cagcaggatg
     3001 atgcccatct tgtcggcgac atccttcacg aactcctggc cgatctcgcc ccggaaggtc
     3061 ttgagcatgt ggtcggcatc acggaacaca taggcaccgc cgagcgcgcc ccactccggc
     3121 agctgcgcct cgatttccgg caccaccggg ctcgccatct ccagattgcc gcgctgcatg
     3181 ccgggcactt ccgtaccctg gcggaacagc gcgctcgccg gatgcaccga gaccttgagc
     3241 tgacccggca acttggcttc cagggttgcc ttgaaccggt tgaacgccgc cgtgaggggc
     3301 gcgctggtcg gcgcggcggt ggaaatgcgg atttcggtct gcgccgaaac cggcggcgcg
     3361 gccaggatgc cggcggcaag cgccagcgct gcgagtctga tcggcttcat gctaggtttc
     3421 ctccctctct gggcattctc aagaaacgac cggcgcgcct cagcgctccg gctggataac
     3481 gacggagagc cggccgatgc cctcgatctc ggcatcgacc gcatccccga ccttcaggaa
     3541 cgtctgtttc ggcaccccga cgcctgcggg cgtcccggtc agcaacaggt caccgggatc
     3601 gagcgtcatg atcctcgagg ccgtcgagat ctgttcggcg atggaaaaga tcatgtctgt
     3661 cgaacggcca tcctgcttca gctcgccatt gacccacagc ttcagcccga tgtcctgcgg
     3721 attggcgatg gctgaggccg gcacaaggcg cggccccatc gggcagcagg tgtcattggc
     3781 tttgcccgcc acccagtcga gcttgtagaa agtctcgggc gcgcggttgt ggtcgcgggc
     3841 ggagaagtcg atggtcacgg tataggccgc gacatgctcc agtgcctttt ccaccggcac
     3901 gttgcgggcg cgtttgccga tgatgcagcc cagctcgatc tcccagtcga aagccttcgt
     3961 gccgatcggc atgtgcaccg tggcgccctc gcccacgacg gcattgcgcg gcggcttcat
     4021 gaagaagaac agccgctggt ccgccttctt cgcgccgggc atgcccattt cggcgagatg
     4081 gtcgaaataa ttcgccccgg cgcagaggat cttgccggga tagagcagcg gcgcgagccg
     4141 gcgcgatggc atcaccgcat ccgccttgtg caggcgcgcc acggcggcat tgagcgccgc
     4201 aagcgcccgc tcccagtcgg cgaacagcgc cgcgatgctg ctccctcctg cgtagcccac
     4261 gcgctccagc gccggctgta ggcgatacag agccccgcct gcctccatgc aggccacggc
     4321 ccgcccctca acctcgaccg tcgccagcgc ccactgcgcc attcccgcct ccggaattcc
     4381 gcttgatatg agcgcgacca tattttataa attcaagtcg tcaacatgaa tctggccatt
     4441 cctcatgagc atcgctgacg gacgttccca ggccgaacgc gcctacaagg tgctgcgtgg
     4501 cgagatcctg cacggcgacc tgatgccggg cgagcgtctg cgcgccgccg acctgcagga
     4561 ccgctataac cttgggctga cgccgatccg tgaggcgctg atgcgcctgt cgtcggaaaa
     4621 cctggtggag gcggagacca accgcggttc gcgcgtcacc gaaacctcgg cccaggaact
     4681 ggcggacctg atggcgaccc ggcgcgccat cgaacgattg tgcctgaccg cgtccatcga
     4741 gcggggggat gcgctctggg aagcgaacat cgtcgcggcg atgcacctgc tctcccgtac
     4801 accgcttccg gtctccgcca gcgaccggga ggcagcgtcg cgctgggagg tgcagcatcg
     4861 cagctttcac ttcgcgctgg tcgcggcctg cggatcaccc tggctgctgc ggttctggaa
     4921 tacgctggcc gaccattcgg agcgctatcg caagctgcgc ctgctgcatc tgcacgaaat
     4981 ccgtgacacc gtgcgcgaca tcaacacgga gcatgccgaa atcatggcgt cggtcctggc
     5041 gcgcgacgtg gcgcggtcca ccgctttgat ggacgaccat ctcgcggcga ccgagaaatg
     5101 ggttgcgcgc cttctggaga cgcagggagg ataggcggcg cgtctgccga tcctaccgga
     5161 acgccggttc gtcgaagctt ctcagctttc gtgtatgcaa ctggtccagc tttccgcgca
     5221 gcagatccac cgccgtgagc ccgatctgca ggtgctggcc caccgcccgt tcatagaagg
     5281 cggaggccgc gccgggcagc ttgatctcgc cgtgcagcgg tttgtcggag acgcagagca
     5341 gcgtgccgta gggaacgcgc agccggtaac cgttcgcggc gatcgtgccc gattccatgt
     5401 ccaccgcgat ggctcgggac aggttgatgc gctggcgttc ctggctccag cgcagttccc
     5461 agttgcggtc gtcgtaggtc accacagtgc cggtgcgcag ccgcgccttg agcttgtcgc
     5521 cgcgctcgcc ggtcaccatc cccgccgcct cctgcagcgc cacctgcact tccgccagcg
     5581 ccggaatggg aatgtccggc ggcaccagcg cgtcgaggat tcggtcctgc cgcagatagg
     5641 catgcgccag cacatagtcg ccgatttcct ggctctgacg caggccgccg cagtgcccca
     5701 ccatcaggaa acagtgcggt ctcagcacgg cgaggtgatc cgtgatgttc ttggcgttgg
     5761 acggcccgac gccgatattg accagcgtga tgccgccgcc cgcgccggcc aggtggtagg
     5821 ccggcatctg gaacttggac caggccgcga tgtcgagcag ggattccagt tccttcccgc
     5881 tcttgggcgt ggtgacctcg acgtttcccg gcagcaccag cttggtgtag tgcgacttgc
     5941 cgccgatctg cgtcatgccc caacgcacga actggtcgac atagcggtgg tagttggtga
     6001 gcaggatgaa gggctgcacg tgcttccagt gggagccggt gtagtgcatc agccgcttca
     6061 gcgaatagtc ggtgcgcgcc gcgtcgaaca gcgccagcgg ccacggtccg gcattggcca
     6121 ggtgaaacgt gccgtccgcg atttcgtccc ccaccgccga gagctgcgga acggggaaga
     6181 accgcgccag ttccgccgcc gtcacgccgc tgcggcccat ctcgtcgccc ttgtcgacca
     6241 cgtagggaaa cgggatctcc tggttcgata gccccacctc gatggtcgcg ccgtattccg
     6301 ccaccagggg ccggagctgt tccagcagat actcccggaa atcgccgggc tgggtaatgg
     6361 tcgaggagaa cacgcccggc cgatggaact tggcgtaggc gcgccggttc gagggcagca
     6421 cgccctgcgg cttgtagctc agccgcagtt cgggatagcg gaaccgcgcg cgctcattgg
     6481 gctgcggagg cacctttttc ttcaggtagc gctccagcga ggcgcgcagc gcgccggtcg
     6541 cctgcgtgtg caggaaaacc agttcgtcca ccgccgcttc cggcgtctcg acccgcttga
     6601 aatcgtccca gaccattcct gtcccctcaa atcagccggg gagtgtggca gcagggcggc
     6661 cgctacgcaa tgccgcaggg atgaagaggc ttgccggcag ggcgtcgcga cccacatgtt
     6721 gccggctatg gattcgctcc cacgaaaacg ccgcagtctg gttctccttg tcgcgctgca
     6781 tctgacggcg ctggtcatcc tgctgctgac cgagacggcg ctcgttccca agctggcctt
     6841 ccttgtgtcc tggctcttct tcaatttcgg ctggatggtg ctgctgcgac gcccggctat
     6901 ggcggccgcg ctggcgctgt tcttcctctg cctgctcatc gtgctgtcga agctcaagca
     6961 cgaaatcctg ctgatgacgg tgaacttcgt cgacatcctg ctgatcgatc ccgacacctt
     7021 cgccttcctg atgcaggtct ttcccgggct tggactgaga tcggccggag cggcgctgtt
     7081 gatcatcggc gcgctggcca tcgtctggcg caacgacgtg ctgcgg
//
LOCUS       DAOMXN010000135         7082 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_5556_length_7082_cov_10.326028, whole genome shotgun sequence.
ACCESSION   DAOMXN010000135 DAOMXN010000000
VERSION     DAOMXN010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 7082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7082
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5556_length_7082_cov_10.326028"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="PK812_09670"
     CDS             <1..561
                     /locus_tag="PK812_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019905081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HRJ69866.1"
                     /translation="GTTAAEDRALEAELLADPKERAEHLMLLDLGRNDTGRVATIGSV
                     TVTDSFFVERYSQVMHIVSNVEGTLAPGRDAIDALVAGFPAGTVSGAPKVRAMEIIDE
                     LERDKRGPYAGCIGYFSADGALDSCIVLRTAIVKDGVMHVQAGAGIVHDSDPQSEQTE
                     CENKARALFRAAETAVRFATQSRRGQ"
     gene            728..1324
                     /locus_tag="PK812_09675"
     CDS             728..1324
                     /locus_tag="PK812_09675"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014130544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HRJ69867.1"
                     /translation="MNLLLIDNYDSFTFNVVHLIGSLGATIQVVRNDELTVEQALARN
                     PDGIVLSPGPCTPNEAGITLPLIQAAADRVPIFGVCLGHQAIGQAFGGHVVRAAPMHG
                     KVSTLTHQGRGLFRGLNGPFRAARYHSLVVERASMPAELEITAGTDDGFVMSVSHRTL
                     PVHGVQFHPESIASEHGATICRNFLELAAEWNKKRRAG"
     gene            1390..2406
                     /gene="trpD"
                     /locus_tag="PK812_09680"
     CDS             1390..2406
                     /gene="trpD"
                     /locus_tag="PK812_09680"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HRJ69868.1"
                     /translation="MDSFKPLIAKIATGMPLSRDEAQHAFSQILSGGSTPAQTGAFLM
                     GLRVRGETVEEIEGAVAAMRAKMVPVTAPAGAIDVVGTGGDASGSWNVSTLAGLIVAA
                     CGVPVAKHGNRAASSKSGAADVLTAVGVKVGIAPEQVEACIRESGFGFMLAPAHHPAM
                     RHVGPVRVEMGTRTIFNLLGPLANPANVKRILIGVFSPTWAEPVAETLRALGCERAWV
                     VHGSDGLDEMTTTGATDVVSLEDGRISRFEVTPEDAGIARANPADLKGGEPGHNAEAL
                     LAVVDGAHTAYRGIAVLNAAGALVVAGKAASLRQAAGMAAEAIDSGKVRKVLDKAIVV
                     SNAA"
     gene            complement(2407..3357)
                     /locus_tag="PK812_09685"
     CDS             complement(2407..3357)
                     /locus_tag="PK812_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012757436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HRJ69869.1"
                     /translation="MSSGLLALLDDVAALAKVAAASLDDVAAQATKAGAKAAGIVIDD
                     AAVTPRYVVGFAAQRELPIIGKIALGSLKNKLLFLLPGALALSYFAPWVITPLLMLGG
                     LYLCYEGAEKVAEAAGFHHGHEKSGDDAEAMDAATFEAEKIAGAIATDFILSAEIMAI
                     ALSTVAGSSMMTQAAVLVVVGLGLTVVVYGAVAIIVKADDVGYALAKDHRGPVARAVG
                     RALVLGMPKVLALLSLVGTAAMVWVGGGILLHGAETLGFGGPAHWAHDIAAATGRLAG
                     ALGGVIEWLVGAGLSGVLGLLVGAALIPLVGVAKKLRGAG"
     gene            3533..5029
                     /locus_tag="PK812_09690"
     CDS             3533..5029
                     /locus_tag="PK812_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRJ69870.1"
                     /translation="MKKHGLAMALAAGLFATTASAQVLQMAVDGSPAGLDPHLVTAFN
                     SIQIVSGTIYEGLTAIDANLRTVPALAKSWDISPDGKTYTFRLVEGATFHNGAKLEAA
                     DVVASLGRVMSKDIASPLASRLASVDKAEAKDAGTVVLSLKEPSAPLLTALASIAIVP
                     RTFESNKDGLQRAPVGSGPFKFKEWQPNGFIELEKHAGYHDKAKPKLAGLKYNIVPES
                     ATRQVGVTSGQYAMLPNIDAATALQLKGKPGVRLAETLELAYTLVGMNVSKPPFDNPK
                     VREALNYALNRSEIVQAALFGAGVPGGPLSPALKDWALDVKEFPCFTHSAAKAQDLLK
                     AAGVTGPVAVTLLVLPRQDIKDIAQVVQAQLNKAGFKAELKIPELGAFVQDWRNSNFD
                     AFVSTNAGSTDPDDYFYRTFRSGGSTNVFKYANADVDKLLDSARAESDAGKRKAAYAS
                     VQKALACDGPAAFMTYGQLFTAMRANVQGFGIIANRSLMTLADTTVGQ"
     gene            5084..6028
                     /locus_tag="PK812_09695"
     CDS             5084..6028
                     /locus_tag="PK812_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ69871.1"
                     /translation="MKLLASRLADLLVVLVGVSVITFLMIRLIPGDAVAIMLGANTEV
                     TPEKIAELRARLGLDQPLVSQYFQWLGGVLSGDLGVSIWTRRPVSQEIGAHIRPTVQL
                     AAMALVFGAGLAVPLGVLMARWRGNRMNALLQIGTVAGLTIPSFWLGIMMILLLSAVA
                     PEAQVLGYVPFTEAPLESLKRLALPAFALALPILANLARLVRSAMLDALAQDYVRTAR
                     AKGVSEASVFFKHALRNALIPFVTSLGIMTGYLLGGAIVVEQVFAIPGLGRLVLGAIA
                     ERNYPLVQAVVLVVTVTFVGVNFLVDLLYAAIDPRVRP"
     gene            6025..6852
                     /locus_tag="PK812_09700"
     CDS             6025..6852
                     /locus_tag="PK812_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ69872.1"
                     /translation="MSGKRPRSGLQLFASGLTLVVLLAAFLGPELLARDPLAQSVLTR
                     LKGPSATHWLGTDQFGRDILVRILHGMRASLTISAVAVFTALAVGGTLGLVAAYYRGW
                     TDRVLMRLMDMLFAFPVMLLAIGIIAMLGPGGGPTAIAIAVVYTPIFARLMRGPALVL
                     VESDFVESARAVGASDARIIFRHILPNLASVILVQTSLLLSAAILVEASLSFLGLGTQ
                     PPRPSLGMMLAEGRNFLMLSPWSAIFSGLAILIVAFALNLLGDALRDALDPRLRGER"
     gene            6849..>7082
                     /locus_tag="PK812_09705"
     CDS             6849..>7082
                     /locus_tag="PK812_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69873.1"
                     /translation="MIIRDAQADDIEVLAAIAAEVYRSVFAPLLPEADLSVFGHAHFA
                     DRFAACLPRVRVIVGGDRPAGLCLLTEGNIDILF"
BASE COUNT         1150 a         2453 c         2271 g         1208 t
ORIGIN      
        1 ggaaccacgg cggcggagga ccgcgcactg gaggccgagc tgctggccga ccccaaggag
       61 cgcgccgaac acctgatgct gctcgacctg gggcgcaacg acaccggacg cgtggcgacg
      121 atcggcagcg tcacggtgac ggactcgttt ttcgtcgagc gctacagcca ggtcatgcat
      181 atcgtctcaa acgtcgaggg cacgctggcg ccgggccggg acgccatcga tgcgctggtg
      241 gccggctttc cggccggcac cgtttccggc gcgccgaagg tccgggcgat ggagatcatc
      301 gacgaactgg aacgggacaa gcgcggaccc tatgccggct gcatcggcta tttcagcgcc
      361 gatggcgcgc tcgattcctg catcgtgttg cgcacggcga tcgtgaagga cggggtgatg
      421 catgtgcagg ccggcgccgg catcgtgcat gattccgatc cgcagtcgga acagacggaa
      481 tgcgaaaaca aggcaagagc cctgttccgg gctgccgaga cagccgtgcg cttcgcgaca
      541 cagtcacgtc gggggcaatg atgcgcggta tcgtttcacg gcagcgttcg cctctgccaa
      601 ggagcggaat cgcggcgctg cggcgggcat gcgtgcccgc accccggtat tgccccttca
      661 cccggcgcct tgcgccgtcc ttcccgtccg ggagcgctga acacggcgct tggcgaaagg
      721 cgcacccatg aatctgctgc taatcgacaa ttacgacagc tttaccttca acgtggtgca
      781 cctcatcggt tcgctcggcg ccacgataca ggtcgtgcgc aacgacgaac tgacggtgga
      841 acaggcgctc gcccgcaacc cggacggcat cgtgctctcg cccggtccct gcacaccgaa
      901 cgaggccggc atcaccctgc ccctgatcca ggccgcggcg gaccgggttc cgatcttcgg
      961 cgtttgcctc ggccatcagg ccattggcca ggcgttcggc ggccacgtgg tgcgcgccgc
     1021 gccgatgcac ggcaaggtct cgaccttgac ccatcagggg cgcggactgt tccggggcct
     1081 gaacggtccg ttccgggccg cacgctatca ctcgctggtg gtggagcggg cgagcatgcc
     1141 agccgagctt gaaatcaccg ccggaacgga cgacggcttc gtcatgtcgg tctcgcaccg
     1201 cactctgccc gttcatggcg tgcagttcca tcccgaaagc atcgcctccg agcatggcgc
     1261 gacgatctgt cggaatttcc ttgaacttgc ggcggaatgg aacaagaagc ggcgtgcggg
     1321 atagtcgcgg acattccttt tcctccgggg acggaccggc ggcggcgcat tgtgcaaacg
     1381 gagcagcgcg tggacagttt caaacccctg atcgccaaga tcgccaccgg aatgccgctg
     1441 tcacgcgatg aggcgcagca tgccttctcg cagatccttt ccggcggctc gactcccgcc
     1501 cagaccggcg cttttctcat gggactgcgc gtgcgcggcg agacggttga ggaaatcgag
     1561 ggcgcggtcg ctgcgatgcg cgcgaagatg gtaccggtca cggcgccggc cggcgccatc
     1621 gatgttgtgg gcacgggcgg agacgcgtcc ggctcatgga atgtctcgac cctggccggc
     1681 ctgatcgtcg cggcttgcgg cgtgccggtc gccaaacacg gcaatcgcgc cgcctcatcg
     1741 aaatccggag ctgccgacgt tctcacggcg gtgggcgtga aggtcggcat cgcgccagag
     1801 caggtcgagg cctgcatccg cgaaagcggc ttcggattca tgctggcgcc cgcccatcac
     1861 cccgcgatgc gccatgtcgg cccggtccgt gtcgagatgg gcacgcgcac catcttcaac
     1921 ctgctgggcc cgctggccaa tccggccaat gtgaagcgca tcctgatcgg cgtcttttcc
     1981 ccgacctggg ccgaaccggt ggcggagacg ctgcgcgccc tcggctgcga gcgcgcctgg
     2041 gtcgtgcacg gctcggacgg gctggacgaa atgaccacga ccggcgcgac ggacgtggtt
     2101 tctctggaag acggccgcat ctcccgtttc gaggtgacgc cggaagacgc cgggatcgca
     2161 cgcgccaacc cggccgacct caagggcggc gaaccgggcc acaacgccga ggcgctgctg
     2221 gccgtggtcg acggcgccca cacggcctat cgcggcatcg ccgttctcaa tgcggccggt
     2281 gcgctggtcg tggctggaaa ggccgcctcg ctcaggcagg ccgccggcat ggccgcggaa
     2341 gcgatcgaca gcggcaaggt gcggaaggtc ctcgacaagg ccatcgtggt gtcgaacgcg
     2401 gcgtagctac cccgcgccgc gcagcttctt cgcgacccca accagcggga tcagcgcggc
     2461 gccgacgagc aacccgagca cgccggacag gccggcgccg acgagccatt cgatcacccc
     2521 gcccagtgcg ccggcgagac gcccggtggc ggccgcgata tcatgcgccc agtgggcagg
     2581 accgccgaat cccagggtct cggcgccatg cagcaggatg ccgccgccga cccagaccat
     2641 cgcggccgtc ccgacaaggc tcagcagcgc cagcaccttg ggcatgccga ggacaagcgc
     2701 acgccccact gcgcgcgcaa ccggaccgcg atgatccttg gcgagcgcgt aaccgacgtc
     2761 gtccgccttc acgatgatcg ccaccgcccc gtagacgaca acggtcagcc ccagcccgac
     2821 aacgaccaag actgcggcct gcgtcatcat ggaggagccc gcgacggtcg atagcgcgat
     2881 cgccatgatc tcggccgaga ggatgaagtc tgtggcgatg gcgccggcga ttttctccgc
     2941 ctcgaacgtg gcggcatcca tagcctcggc gtcgtcgccg gacttctcat ggccgtggtg
     3001 gaaaccagcg gcctcggcaa ctttctccgc gccctcgtag cacaggtaga gccccccgag
     3061 catcagcagc ggcgtgatga cccagggtgc gaaataactg agcgccagcg cgccgggcag
     3121 caggaacagc agcttgttct tcagcgatcc cagcgcgatc ttgccgatga tcggcaactc
     3181 acgctgcgcg gcgaagccca cgacatagcg tggcgtcacg gcggcatcgt cgatgacgat
     3241 ccccgcggcc ttggcgcctg ccttggtcgc ctgggcagcg acatcgtcca gcgaggccgc
     3301 cgccaccttg gcgagggcgg cgacatcatc aagcagcgca agcaatccgg aagacacagc
     3361 gcgaatccct catttttcca ggacagcatt tggacaacct tccggccaag acaagcgcct
     3421 gtcttgcaat tggccggggc cggacttacg actttcagaa caggggatcg ggtggcgtat
     3481 gcggacagtg aggcccgcgc atctcccggt ttggcgttct cgggaggaca acatgaaaaa
     3541 gcatggtctg gccatggccc tcgcggctgg cctgttcgcg acaaccgctt cggctcaggt
     3601 ccttcaaatg gcggtcgatg gttcgccggc cggtctcgac ccccatctcg tcaccgcctt
     3661 caattcgatc cagatcgtct caggcacgat ctacgaggga ctgacggcca tcgacgccaa
     3721 cctgcgtacg gtcccggccc tggcgaaatc atgggacatt tcgcccgacg gcaagaccta
     3781 tacgttcagg ctcgttgagg gcgccacttt ccacaacggc gcgaagctgg aggccgccga
     3841 tgtcgttgcc tcgctcggcc gcgtcatgtc gaaggacatc gcctcgccgc tcgccagccg
     3901 cctcgcctcg gtcgacaagg ccgaagcgaa ggacgccgga acggtcgtgc tgagcctgaa
     3961 ggagccgtcc gcgccgctgc tcacggccct ggcctcgatc gccatcgtgc cccggacgtt
     4021 cgaatccaac aaggatggcc ttcagcgcgc gcccgtcggc tccggaccgt tcaagttcaa
     4081 ggaatggcag cccaacggct tcatcgaact ggaaaagcac gcgggctacc acgacaaggc
     4141 gaagccgaag ctcgccggcc tgaaatacaa catcgtaccg gaatcggcga cgcgtcaggt
     4201 cggcgtcacc tccggccagt acgcgatgct gccgaacatc gacgcggcaa ccgcgctgca
     4261 actcaagggc aagcccggtg tcaggctggc cgagacgctg gaactggctt acaccctcgt
     4321 cggcatgaac gtctcgaagc cgcccttcga caatccgaag gtgcgcgagg cgttgaacta
     4381 cgcgctcaac cgcagcgaga tcgtgcaggc tgccctgttc ggcgccggcg ttccgggcgg
     4441 accgctgtca cccgccctca aggactgggc cctcgacgtc aaggaattcc cctgtttcac
     4501 ccattcagcg gcgaaggcgc aggacctttt gaaggctgcc ggcgtaaccg ggcccgtggc
     4561 ggtgacgctc ctcgtgctgc cgcgtcagga catcaaggac attgcgcagg tcgtgcaggc
     4621 ccagctcaac aaggccgggt tcaaggcgga actgaagatc cccgaactcg gcgccttcgt
     4681 gcaggactgg cgcaactcca atttcgacgc cttcgtctcc actaacgccg gctcgaccga
     4741 tccggatgac tatttctacc gcaccttccg cagcggcggc tccaccaatg tgttcaagta
     4801 cgccaacgcg gatgtcgaca agctgctgga ttcggcgcgg gcggaaagcg acgcaggaaa
     4861 gcgcaaggcc gcctatgcca gcgtgcagaa ggctttggcc tgcgacgggc cggccgcctt
     4921 catgacctac ggccagctgt tcacggccat gcgcgccaat gtgcagggct ttggcatcat
     4981 cgccaaccgc tcgctgatga cgctcgccga cacgaccgtg ggccagtgac cgtcctgacc
     5041 cggacagagc ctgcccgggc cggcgccgcg acctgaccgc gtcatgaagc tgcttgcctc
     5101 ccggctcgcc gatctgctgg tggtgcttgt cggcgtctcg gtcatcacct tcctgatgat
     5161 tcgcctgatc ccgggcgacg ccgttgccat catgctcggc gccaacaccg aggtcacgcc
     5221 ggagaagatt gccgaactgc gcgcccggct ggggctggat cagcctctgg tcagccagta
     5281 tttccagtgg cttggcggtg ttctttccgg cgatctcggc gtctcgatct ggacgcgccg
     5341 tccggtgtca caggaaatcg gcgcgcacat ccgccccacg gtccagctcg ccgcgatggc
     5401 actggtcttc ggcgccggtc tcgcggtgcc gctcggcgtg ctcatggcgc gctggcgcgg
     5461 caacaggatg aacgcgctgc tgcagatcgg caccgtggcc ggcctgacca tcccctcttt
     5521 ctggctcggc atcatgatga tcctgctgct gtccgccgtg gcgccggaag cgcaggtgct
     5581 gggctacgtg ccgttcacgg aagcgccact ggaaagcctg aagcggctcg ccctgcccgc
     5641 cttcgcgctc gccctgccga tcctggcgaa cctagcccgg ctcgtgcgct ccgccatgct
     5701 cgatgccctg gcgcaggatt acgtgcgcac ggcgcgcgcc aagggcgttt cggaagcctc
     5761 cgtcttcttc aagcacgcgc tgcgcaacgc gctgatcccc ttcgtgacga gcctgggcat
     5821 catgaccggc tatctgctcg gcggcgcgat cgtcgtcgaa caggtcttcg caattcccgg
     5881 cctcgggcgc ctggtgctgg gcgcgatcgc cgagcgcaat tatccactcg tgcaggcggt
     5941 cgtgctcgtc gtgaccgtca ccttcgtcgg cgtcaacttc cttgtcgacc tgctttatgc
     6001 cgccatcgat ccgcgtgtgc gcccatgagc ggcaagcgcc cacgcagcgg attgcagctc
     6061 ttcgccagcg gcctgacact ggtcgtgctg ctggccgcct tcctcggccc ggaactgctg
     6121 gcgcgcgatc cgctggcgca gagcgtgctg acgcgactca agggtcccag tgccacgcac
     6181 tggctcggca cggaccagtt cggtcgcgac attctcgtgc gcatcctgca cggcatgcgc
     6241 gcctcgctga cgatttccgc ggtcgccgtc tttaccgcgc tcgccgtggg cggcacgctg
     6301 ggcctcgtcg ccgcctacta ccggggctgg acggaccggg tgctgatgcg ccttatggac
     6361 atgctgttcg ccttcccggt gatgcttctg gccatcggca tcatcgccat gctgggcccc
     6421 ggcggcggac cgacggccat cgccatcgct gtggtctata ccccgatctt cgcgcgcctc
     6481 atgcgcggtc cggccctggt gctggtggaa agcgactttg tcgaaagcgc gcgcgcggtc
     6541 ggcgcttccg atgcccgcat catctttcgc catatcctgc cgaaccttgc cagcgtcatt
     6601 ctggtgcaga ccagcctgct gctctcggca gccattctgg tcgaggcctc gctctccttc
     6661 ctcggcctgg gcacgcaacc gccgcgcccg tcgctcggca tgatgctggc ggaagggcgg
     6721 aatttcctga tgctgtcacc ctggagcgcc atcttctcgg gcctcgccat tctcatcgtg
     6781 gccttcgcgc tcaacctcct cggcgacgcc ttgcgcgatg cgctcgaccc gcgcctgaga
     6841 ggcgagcgat gatcatccgc gacgcgcagg ctgacgacat cgaggttctg gccgcgatcg
     6901 ccgcggaggt ttaccgttcg gttttcgctc cgctgctgcc ggaagccgat ctttccgtct
     6961 tcggtcatgc ccacttcgcc gaccgcttcg ccgcctgcct gccccgcgtc cgggtgatcg
     7021 ttgggggcga ccggcccgcc ggcctatgtc tgctgacgga aggcaacatt gatatcctgt
     7081 tc
//
LOCUS       DAOMXN010000136         7078 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_5561_length_7078_cov_10.018368, whole genome shotgun sequence.
ACCESSION   DAOMXN010000136 DAOMXN010000000
VERSION     DAOMXN010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 7078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7078
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5561_length_7078_cov_10.018368"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            271..1947
                     /locus_tag="PK812_09710"
     CDS             271..1947
                     /locus_tag="PK812_09710"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HRJ69874.1"
                     /translation="MDLPARERMEYDVVVVGGGPAGLSTAIRLKQLAAEAGSDLSVVV
                     VEKGSEVGAHILSGAVIDPIGLDRLIPDWRSDETCPVKTAVTADHFLFLGPAGGVRLP
                     NFAMPKLMNNHGNYIISLGNLCRWLATQAEALGVEIYPGFAASELVRDDKGAIIGIAT
                     GDMGVARDGSLKDSYTRGMELTGKYVVFAEGARGQLTKELIRDFALDAGREPAKFGIG
                     LKELWQVAPEKHRKGLVQHSFGWPLDSRTGGGSFLYHLEDNLVAVGFVVHLNYENPTL
                     SPFDEFQRFKTHPMIRDTFEGGKRLSYGARAITEGGYQSVPKLSFPGGVLVGCAAGFV
                     NVPRIKGSHNAMLSGMLAAEHIAKALAEGRANDELLSYEQAWRASEIGKDLWKVRNAK
                     PLWSKFGTWIGVTLGGIDMWCNELFRFSPFGTLKHGKPDSATLKPLSEVTPITYPKPD
                     GVVSFDKLSSVFLSSTNHEEDQPVHLTLADPSKPVSENLPRYGEPARLYCPAGVYEVV
                     YGDEAAKKDPRFVINAQNCVHCKTCDIKDPARNITWVAPEGGGGPNYSGM"
     gene            2037..2261
                     /locus_tag="PK812_09715"
     CDS             2037..2261
                     /locus_tag="PK812_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69875.1"
                     /translation="MSQPVEAFGRTIQLLLVDGTPNGLTIASIHGWTGSVLVASQSTF
                     AKLLSRAEVDRTGVYILFGPDPSDALSMRA"
     gene            2303..2935
                     /locus_tag="PK812_09720"
     CDS             2303..2935
                     /locus_tag="PK812_09720"
                     /inference="COORDINATES: protein motif:HMM:NF025625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HRJ69876.1"
                     /translation="MITTSDEALTKGHVRYLEARLIELAISAGRVRLDNGQAPSADKR
                     RMPEADRANMEAFLANLKTVLPVIGLDLLKPRPASATKVAGQGIAANEPRFEIRHKSG
                     VKATAVEEEGEFIVLQGSEALKDAGYQGTNGYGELRQELVAQGVLSTTPEAKFYTFAK
                     PYAFKSPSAAAAVVLDRNSNGRWEWKLVGSKLTYHKWQEEKAQVTANPTP"
     gene            complement(2994..3386)
                     /locus_tag="PK812_09725"
     CDS             complement(2994..3386)
                     /locus_tag="PK812_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRJ69877.1"
                     /translation="MVAFLDTNILLYAQERGAKAEKARALIDAGGVTSVQSMNELINA
                     LIGKYRRPWNEVERIRDDLLATLGLPRPLTFATHLFAANVARDHKFHIYDAMIIASAI
                     EAGCDTLYSEDMHDGWTLAGLTVVNPFG"
     gene            complement(3380..3625)
                     /locus_tag="PK812_09730"
     CDS             complement(3380..3625)
                     /locus_tag="PK812_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HRJ69878.1"
                     /translation="MQVSKWGNSLAVRLPKALVEELGLKEGDKIDLVKAAPGQFAVAK
                     DTRRAEALARMAARGLERPADFKFDREEANRRPGRKW"
     gene            complement(3689..4744)
                     /locus_tag="PK812_09735"
     CDS             complement(3689..4744)
                     /locus_tag="PK812_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRJ69879.1"
                     /translation="MNRRHLLALFGLTATGAAAYSTTMPSKNPYYSGPPSPHFDGLRF
                     GLPGAVADKSRTDLLKWQVTGSKARWPSAYPTPGFDRPPERVAGSALRVSYVGHASFL
                     VQTEGLNILIDPVWSQRASPFQWAGPRRVNDPGIALADLPPIDVILVSHNHYDHLDLA
                     TLSALTRGHQARVITPLGNDTILLGHDSSIRVSAHDWSERIRISERVAVHIEPAYHWS
                     ARGLGDRRMALWCAFVIETPAGNIYSVCDTGYRDGAIFRAMKAKHGGFRLALLPIGAY
                     APRWFMADQHIDPFEAVKILKETNSAAAIAHHWGTFQLTDEAIDEPPKLLAEALEAAN
                     LPPEVFAVLRPGGVWEG"
     gene            4942..6765
                     /locus_tag="PK812_09740"
     CDS             4942..6765
                     /locus_tag="PK812_09740"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ69880.1"
                     /translation="MPFADTAVHPQARFARMNRRAPLAALALLLFAGTALAASPRESA
                     VMRQEALRGTSGSFLAALHAQQLRDVRPAGEFFGDVLKHDSRNPALLERAFVSELGDG
                     DFTAAFRLAERVIQQDPANPLAQAALGVRALKNKQYKTARSFLGKAGSARGRRADLTV
                     ALLTAWTQVGSGELKRALEIVDKFNQPELAAYRNFFGGLMADVAGDKAEASRRLVSAY
                     KSEPGTLRVADAHARQLSREGNREQALQIYAEWESRNPGQPFVRQQIADLKAGKTLAP
                     LATSVADGAAEILYGIGIASGGGRDSDSSLIFLQLAAYLAPEDELIRVSVAEVFEQMR
                     QWARAGEAYATIPNGSPFRIRALLGRVISFERQEKTDDAIRTLTTLLGESPDELDAVD
                     MLGGLYRSKKKMSESIAVYSKAIDRLKTPERSHWNLYFGRGVAFERNKEWPKAEVDFL
                     KALELLPDTVRTPREKFDRAQVLNYLAYSWVDQGMHIERSFEMLKRAVELAPEDGAIV
                     DSLGWAYYRLGKFEDAVRELEKAVGLKPGDPTINDHLGDAYWQVGRRTEAHFKWNHAR
                     DLKPEPDDLPKILRKIEVGLEDEKPMALPAELAPPQKSNGG"
     gene            6755..>7078
                     /locus_tag="PK812_09745"
     CDS             6755..>7078
                     /locus_tag="PK812_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012322242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HRJ69881.1"
                     /translation="MVAERGREIVAQAPAKINLSLHVLGRRADGYHELESLVVFADLA
                     DTLTFVPGATPSLVVTGPTAAASGPVSDNLVLKAVSALVARVPGLMTGALMLDKKLPA
                     AAGIGG"
BASE COUNT         1283 a         2274 c         2303 g         1218 t
ORIGIN      
        1 accggctccg ccgcgccctc ggcgaagcga tcgtgcggcg catcgtccag cgcgacatcg
       61 accccggcct gcacatacca ttcgaccagg tcgcgcaggg cgtcgatctc gctccgggag
      121 gaaccactgc cggtcggcat tccttgctct ctgctccctt ttcaggcgac cttatgcgag
      181 gtaacgagcc aatccaacgt ttgcgtcgcg ggcgcaagcg tggtagatag ttcacatata
      241 aactatattg cccaacgagg agccgaagcc atggacctgc cggcgcgcga gcggatggaa
      301 tacgatgtgg tcgtcgttgg cggcgggccg gcgggattgt ccacggccat ccggctgaaa
      361 cagctggccg ccgaggccgg cagcgacctg tccgtcgtcg tggtcgagaa gggctccgag
      421 gtcggcgcgc atatcctttc cggcgcggtc atcgatccga tcggcctgga caggctgatc
      481 ccggactggc gcagcgacga gacctgcccg gtgaagaccg cggtgacggc ggaccatttc
      541 ctcttcctcg gcccggccgg aggcgtgcgc ctgccgaact tcgccatgcc gaagctgatg
      601 aacaaccacg gcaattacat catctcgctg ggcaacctct gccgctggct ggcgacgcag
      661 gccgaggcgc tgggcgtcga gatctatccg ggcttcgcgg cgagcgagct ggtgcgcgac
      721 gacaagggcg caatcatcgg catcgccacc ggggacatgg gtgtcgcccg cgacggatcg
      781 ctcaaggact cctacacgcg cggcatggag ctgacgggca agtatgtcgt cttcgcggaa
      841 ggcgcgcgcg gccagctcac caaggaactc atccgcgact tcgcgctgga cgcgggccgt
      901 gagccggcca agttcggcat cggcctgaag gaactctggc aggtcgcgcc ggaaaagcac
      961 cggaaggggc tggtgcagca ctccttcggc tggccgctcg acagccgcac cggcggcggc
     1021 tccttcctct atcacctgga ggacaatctc gtcgccgtcg gattcgtcgt gcatctcaat
     1081 tacgagaatc cgacgctctc gcccttcgac gagttccagc gtttcaagac gcatccgatg
     1141 atccgcgaca cgttcgaggg cggcaagcgc ctgtcctacg gcgcccgggc catcaccgag
     1201 ggcggctacc agtccgtgcc gaaactgtcg tttccgggcg gcgtgctggt gggctgcgcc
     1261 gccggcttcg tcaacgtgcc gcgcatcaag ggcagccaca acgccatgct ctccggcatg
     1321 ctcgcggccg agcacatcgc gaaagcgctg gcggagggcc gcgccaacga cgaactcctc
     1381 tcctacgagc aggcctggcg cgcctccgag atcggcaagg acctgtggaa ggtccgcaac
     1441 gccaagccgc tgtggtcgaa gttcggcacc tggatcggcg tgacgctcgg cggcatcgac
     1501 atgtggtgca acgagctgtt ccgcttctcg cccttcggca cgctgaagca cggcaaacct
     1561 gacagcgcca cgttgaagcc cctttccgag gtgacgccca tcacctaccc gaagccggac
     1621 ggcgtggtgt ccttcgacaa gctctcctcg gtgttcctct cctcgaccaa tcacgaggag
     1681 gaccagccgg tgcacctgac cctcgccgat ccgtcgaagc cggtgtcgga gaacctgccg
     1741 cgctatggcg agccggcccg gctctactgc cccgccggcg tctacgaggt ggtctacggc
     1801 gacgaggcgg cgaaaaagga cccgcgcttc gtcatcaacg cgcagaactg cgtgcactgc
     1861 aagacctgcg acatcaagga cccggcacgg aacatcacct gggtcgcgcc ggaaggcggc
     1921 ggcggaccga attattccgg gatgtgaggg gggatggcgt ccgttgctgg tagtttggtt
     1981 ggcttagttg ctgagcccga aattgccgca gttttctgtt cgcagaaaga ggattcatga
     2041 gccagcccgt agaagcattt ggtcggacga ttcagctctt gctggttgac gggacgccga
     2101 acggattgac gatcgcatct atccacggct ggaccggctc ggtcttagtc gccagtcagt
     2161 cgacgtttgc aaagctgctc tcgcgggcgg aagttgaccg gactggtgtc tatattctct
     2221 tcggtcctga cccttcagat gcgctttcca tgcgggccta acccaaagcg ccgcggatcg
     2281 agggttctgg gaaactgccg tcgtgattac tactagcgac gaagccctga ccaaagggca
     2341 cgtccgttat ctggaggctc gactgattga acttgcgatc agcgctgggc gggtaagatt
     2401 ggacaatggc caggcgccga gcgctgacaa gcgcagaatg ccagaagctg accgggcgaa
     2461 tatggaggca ttcctcgcaa atttgaagac cgttttgccg gtgattggtc tggatttact
     2521 caagccgcgt cccgcttccg cgaccaaagt ggccgggcag ggaattgccg cgaatgagcc
     2581 aaggttcgag attcgacaca agagcggcgt caaggcgacc gctgtcgaag aagaaggtga
     2641 gtttattgtc cttcaaggca gtgaagcgct caaggatgcc ggctatcagg gtacgaatgg
     2701 ctatggtgag ctgaggcagg aacttgttgc ccaaggcgtt ctcagcacga cgccggaagc
     2761 gaagttttat acttttgcca agccttatgc ctttaagagc ccgtctgccg ccgccgcagt
     2821 tgtccttgat cgaaattcca atgggcgctg ggaatggaaa ttggtcggtt caaaactgac
     2881 ttaccataag tggcaggaag aaaaggcgca ggtgactgca aacccgactc cttagccgtt
     2941 gtaggcccgg caattttggc gacaggacat caattcgctt ttccctcttc ccctcaccca
     3001 aacggattga ccaccgtcag gcccgcaagc gtccagccgt cgtgcatatc ctcgctgtag
     3061 agcgtgtcgc aacccgcctc gatggccgag gcgatgatca tcgcgtcgta gatgtggaat
     3121 ttgtggtcgc gggcgacatt tgccgcgaaa agatgtgtgg caaaggtgag cggcctcggc
     3181 aatccaagcg tcgccaacag gtcgtcgcga atgcgctcga cctcgttcca aggacgccga
     3241 tatttaccga tcagcgcatt gatcagctca ttcatcgact ggacgctggt cacaccgcct
     3301 gcatcgatga gcgcgcgcgc cttttcggcc ttcgctccgc gttcctgcgc gtagaggaga
     3361 atattggtgt cgaggaacgc taccacttcc ggcccggccg acggttcgcc tcttcgcgat
     3421 caaacttgaa atcggcgggc cgctccagtc cccgtgcagc catgcgggcg agggcttcgg
     3481 cacgccgagt gtccttcgca accgcgaact ggccaggcgc ggccttcacc agatcgatct
     3541 tgtcgccttc cttcaagcca agctcttcaa ccagcgcctt gggcaggcgc acggccaggc
     3601 tgttgcccca tttcgagacc tgcaccgaag cctccatgat ctacaaatgc aatgtatatc
     3661 atagcggtcg ctatcggcca acccccgcct acccctccca aaccccgccg ggccgcagca
     3721 ccgcaaaaac ctccggcggc aggttcgccg cctccagcgc ttcggccaac agcttcggcg
     3781 gctcgtcgat ggcctcgtcg gtgagctgga acgtgcccca gtgatgcgcg atggcggcgg
     3841 cggaattggt ctccttgagg attttcaccg cctcgaaggg atcgatgtgc tggtccgcca
     3901 tgaaccagcg cggcgcgtag gccccgatgg gcaacagggc gaggcggaag ccgccgtgct
     3961 tggccttcat ggcccggaag atcgcgccgt cgcgatagcc ggtgtcgcag acggaataga
     4021 tgttgccggc cggcgtctcg atcacgaagg cgcaccacag cgccatgcgc cgatccccaa
     4081 ggccgcgcgc cgaccagtga taggccggtt cgatgtgcac ggcgacgcgc tcgctgatcc
     4141 ggatgcgctc gctccagtca tgggccgaaa cgcggatcga ggaatcatga ccaaggagga
     4201 tcgtgtcgtt gccgagcggg gtgatgacgc gtgcctgatg accgcgcgtc agcgccgaga
     4261 gcgtcgccag atccaggtga tcatagtggt tgtgggaaac caggatcacg tcgatcggcg
     4321 gcaggtcggc aagcgcgatg ccgggatcgt tgacgcgcct cggccccgcc cactggaacg
     4381 ggctcgcgcg ctgtgaccag accggatcga tgaggatgtt gagcccctcg gtctgcacga
     4441 gaaagctggc atggccgacg tagctgaccc gcaacgcgct gcccgcgacg cgctcgggcg
     4501 gcctgtcgaa gcccggcgtg ggataggcgg agggccagcg cgccttgctg ccggtgacct
     4561 gccatttcag caggtccgtg cggctcttgt cggcgaccgc gcccggcagg ccgaagcgca
     4621 gaccgtcgaa gtggggggaa ggcggtccgg agtagtaggg atttttcgag ggcatcgtcg
     4681 tggagtaggc cgccgcgccg gttgcggtga gcccaaagag agcaaggaga tggcggcggt
     4741 tcatcgcgcg atagcgtagc gccgggccgg gactgcgaca atggacgtct tgacatcgac
     4801 ggcagcagat atcacttccc cgccatcggc cggctgcggc gtagccagcg cttgcccgat
     4861 tcagccaaaa ccgccatggt gcgggccgat ttgcagccag tgcgtccgat tccggacgcc
     4921 ggtcggagcc ggagtcccga tttgcccttc gctgacaccg cagtgcatcc gcaggcccgg
     4981 ttcgcccgga tgaaccgccg cgcgcccctc gccgccctcg cgctcctgct gtttgcgggc
     5041 acggcccttg ccgcgtcgcc gcgcgaaagc gccgtcatgc gccaggaagc gctgcgcgga
     5101 acatcgggaa gtttcctggc cgcgctgcat gcgcagcaat tgcgggatgt gcgccccgcc
     5161 ggcgagtttt tcggcgatgt gctgaagcac gattcacgca accccgcgct cctggagcgc
     5221 gccttcgtct cggaactggg ggatggcgac ttcacggcgg ctttccggct ggccgagcgc
     5281 gtgatccagc aggatccggc caatccgctg gcacaggcgg cgctcggtgt ccgggcgctg
     5341 aaaaacaaac agtacaaaac cgcccggtcc ttcctcggca aggccggaag cgcccgcggc
     5401 cgacgcgccg atctcacggt cgcgctgctg acagcctgga cgcaggtcgg gtccggcgaa
     5461 ctcaagcgcg ccctcgagat cgtcgacaag ttcaatcagc cggaactggc ggcctatcgc
     5521 aacttctttg gcgggctgat ggccgatgtg gccggcgaca aggccgaagc atcgcgccgc
     5581 ctggtttcgg cctacaagtc cgagccgggc acgctgcgcg tcgccgatgc tcatgcgcgc
     5641 cagctctcgc gcgagggcaa ccgcgagcag gcgctgcaga tctacgccga atgggagagc
     5701 cgcaatcccg gacaaccctt cgtccggcag cagatcgccg acctgaaggc gggcaagacg
     5761 ctggcgccgc tggcgaccag cgtggcggat ggcgccgccg aaatcctcta cgggatcggg
     5821 attgcctccg gcggcggccg cgattcggat tcctcgctga tcttcctgca actggccgcc
     5881 tatcttgccc cggaagacga gctgatccgc gtctcggtgg cggaggtctt cgagcagatg
     5941 cgccagtggg ctcgcgcggg cgaagcctat gccaccattc cgaacggatc gccgttccgc
     6001 atccgggccc tgctcggccg ggtcatctcc ttcgagcggc aggagaagac ggacgacgca
     6061 atccgcacgc tgaccacgct gctgggcgaa tccccggacg aactcgacgc tgtcgacatg
     6121 ctcggcggcc tctaccgcag caagaagaag atgtcggaat cgatcgccgt ctacagcaag
     6181 gcgatcgacc ggctgaagac gccggagcgt tcgcactgga acctgtactt cggacgcggc
     6241 gtcgcattcg agcgcaacaa ggaatggccg aaggcggagg tcgacttcct caaggcgctg
     6301 gagctgctgc cggacaccgt gcgcaccccg cgcgaaaaat tcgaccgtgc gcaggtgctc
     6361 aactacctcg cctattcctg ggtcgatcag ggcatgcata tcgagcgctc gttcgagatg
     6421 ctgaagcggg cggtggaact ggcgccggag gatggcgcca tcgtcgacag tctcggctgg
     6481 gcctattacc gcctcggcaa gttcgaggat gcggtgcgcg aactggagaa ggccgtcggg
     6541 ctcaagccgg gcgatcccac catcaacgac cacctcggcg atgcctactg gcaggtcggc
     6601 cgcaggaccg aggcccattt caagtggaac cacgcccgcg atctgaaacc ggaacctgac
     6661 gacctgccga aaatcctgcg caagatcgag gtagggctgg aggacgagaa gcccatggcg
     6721 ctgcccgccg aactcgcccc gccgcaaaag tccaatggtg gctgagcgcg ggcgggaaat
     6781 cgtcgcgcag gctcccgcca agatcaatct cagcctgcat gtgctcggtc ggcgcgccga
     6841 cggctatcac gagctggaga gccttgttgt cttcgccgat ctcgccgaca cgctgacctt
     6901 cgtgcccggg gcgaccccgt cgctggtggt caccggaccg acggcggcgg ccagcggccc
     6961 ggtctcggac aatctcgtgc tgaaggccgt ttcggccctg gtggcgcggg tgcccggtct
     7021 gatgaccggc gcgctgatgc tggacaagaa actgccggcc gcggcgggca tcgggggc
//
LOCUS       DAOMXN010000137         7070 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_5565_length_7070_cov_11.841340, whole genome shotgun sequence.
ACCESSION   DAOMXN010000137 DAOMXN010000000
VERSION     DAOMXN010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 7070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7070
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5565_length_7070_cov_11.841340"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="PK812_09750"
     CDS             complement(<1..235)
                     /locus_tag="PK812_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HRJ69882.1"
                     /translation="MTTPLPLHAALIDQFKGLIGPEHVLTEPGDRAPYEHEMRGLFPG
                     DAIAVLRPGSTADVAACVRAAAREPHAPFLLQRM"
     gene            complement(267..986)
                     /locus_tag="PK812_09755"
     CDS             complement(267..986)
                     /locus_tag="PK812_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012319094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease activity regulator RraA"
                     /protein_id="HRJ69883.1"
                     /translation="MSGLKPATRDRLKTVSTATLCTALFKRGLRNQFIQDVRPLNPDL
                     PTMVGEAFTLRYIPAREDRNPITVFQNRAHPQRKAIEECPPGAVMVFDSRKDARAASA
                     GSILVSRLMVRGVAGVVTDGGFRDAPEIARLAIPAYHSRPSAPTNLTLHEALDINVPI
                     GCGDVAVFPGDVLVGDGEGVVVIPAEIADEIADEAVEMTAFEDFVTDKVLEGRSILGL
                     YPPTDEQSKVDFAAWRKSKGR"
     gene            1259..1831
                     /locus_tag="PK812_09760"
     CDS             1259..1831
                     /locus_tag="PK812_09760"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69884.1"
                     /translation="MPLFDTSEIIITNPQNIPSLPSNAEYGTSGNDYITLSQPKLVFA
                     GAGNDIVFGSTGADTVYGGLGDDIIVGGAGDDKLYGDSGNDRLYAGTGSDSMYGGAGN
                     DRLYVDPNDNTGALVDLANGGSGIDTLVITNATAGSLRLFSVEGIAGAIVHWTETVGG
                     QTIAQSITVEGVEFVEFNGAAIALQNIQPY"
     gene            2000..2998
                     /gene="paaA"
                     /locus_tag="PK812_09765"
     CDS             2000..2998
                     /gene="paaA"
                     /locus_tag="PK812_09765"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit A"
                     /protein_id="HRJ69885.1"
                     /translation="MYSQALNVADLGGNEEDEGALARFQVRIDAEDKIEPNDWMPEKY
                     RRTLVRQISQHAHSEIVGMLPEGNWITRAPSLKRKAALLAKVQDEGGHGLYLYSAAET
                     LGVSREELVEQLHSGKAKYSSIFNYPAISWADMGMIGWLVDGAAIMNQIALCRCSYGP
                     YSRAMIRICKEESFHQRQGYEIVTTLMRGSQKQRDMAQDSLNRWWWPALMMFGPSDKD
                     SMHSDSSMHWKIKRFSNDNLRQKFVDATVPQGHYLGLKFPDPKLRLDEKTGHWEFGEI
                     DWAEFKRVISGDGPCNRQRMKDRVAAHESGAWVRAAAEAHAEKRAARRNAARMAAE"
     gene            3004..3291
                     /gene="paaB"
                     /locus_tag="PK812_09770"
     CDS             3004..3291
                     /gene="paaB"
                     /locus_tag="PK812_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit B"
                     /protein_id="HRJ69886.1"
                     /translation="MSNKNWPLWEVFIRARNGMAHRHCGSLHAVDAEMALQNARDCYT
                     RRGEGTSIWVVPSNAITASDPAEKDMMFEPTASKVYRHPTFYEVPEEVGHM"
     gene            3300..4052
                     /gene="paaC"
                     /locus_tag="PK812_09775"
     CDS             3300..4052
                     /gene="paaC"
                     /locus_tag="PK812_09775"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaC"
                     /protein_id="HRJ69887.1"
                     /translation="MSTPLFDYTIRLADTALVLGHRLSEWCGHAPILEEDMALANLAL
                     DLIGQSRSFYDYAGAVEGKGRDENALAYLRLEPEWKNLLIVEQPNGDFAATILRQFLV
                     AAFLHPFYRRLSGSKDATLAAIAAKAEKEMAYHVRHCGEWVIRLGDGTAESHRRISEA
                     LADLWPFTGEMFEVDAGDRALIEQGIAVDAVALRAEWEQLVDRVFAEATLERPGDSWM
                     QTGGRNGRHTEHLGHLLAELQYMQRAHPGLTW"
     gene            4058..4573
                     /gene="paaJ"
                     /locus_tag="PK812_09780"
     CDS             4058..4573
                     /gene="paaJ"
                     /locus_tag="PK812_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaJ"
                     /protein_id="HRJ69888.1"
                     /translation="MATAMDAVSPDFLRRKAWTAAATVVDPEVPVLSIADLGVLRDVT
                     FEGGTVVATITPTYSGCPAMNLIALEVDLALEKAGFTRRRVDLKLAPAWTTDWMSAEG
                     REKLRAYGIAPPRPRSASGRGALFGVEDIACPKCGSDNTERISEFGSTACKALWRCKA
                     CREPFDYFKCI"
     gene            4585..5649
                     /gene="paaK"
                     /locus_tag="PK812_09785"
     CDS             4585..5649
                     /gene="paaK"
                     /locus_tag="PK812_09785"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase/reductase subunit
                     PaaK"
                     /protein_id="HRJ69889.1"
                     /translation="MSRFNALTVSDIRRETPQAVSIAFRVPADLSGDYRFEHGQYLTL
                     RRDIGGQDVRRSYSICTGTEDGELRIAVKEVEGGLFSTFVNRDLAVGDTLEVMTPMGR
                     FTAPIEPEAAKTYVGIAAGSGITPLMSIIRTVLQREPKSQFVLLYGNRTANSVIFKTE
                     LEDLKDRFLSRLTVYHVLSREAQDVDLLHGRLEGTRISRLVRSALGDEAIDHAFICGP
                     EAMTESARQALVNLGVQAERIHTELFTTTTPARPRPSVAPKAGDEIALTVTFDGATHA
                     LTIGPDETVIDAAARQGVDLPYSCKGGMCCTCRAKLTEGEVKMDHQFSLEPWELEAGY
                     VLTCQSRPVKGPVALDYDHA"
     gene            complement(6118..6831)
                     /locus_tag="PK812_09790"
     CDS             complement(6118..6831)
                     /locus_tag="PK812_09790"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HRJ69890.1"
                     /translation="MHATRRMFLLGAAATAVSFGRAQAQAPAGPFVQAPLGYAFDALE
                     PHIDAKTMETHFLRHHGAFIGNLNTFAKDHGALGTTPVETLLAGWQQLPEAARVGMKN
                     NLGGHANHTMFWQVMAPGGAKEPGGEVKAAIDAAFGGFDKLKEQFNGSGLRVFGSGWV
                     FVTVDKDGKLALTTRPNQDSPLMDGQRVLFGNDVWEHAYYLKYQNRRNEYLATWWNVV
                     NWTKVGERYAAAKAGTLTI"
     gene            6971..>7070
                     /locus_tag="PK812_09795"
     CDS             6971..>7070
                     /locus_tag="PK812_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family rRNA
                     (cytidine-2'-O)-methyltransferase"
                     /protein_id="HRJ69891.1"
                     /translation="MIRRMRSDRLLVERGVFDSRARAQAAIAAGLVR"
BASE COUNT         1234 a         2333 c         2300 g         1203 t
ORIGIN      
        1 tcatgcgctg gagaagaaat ggggcgtgtg gttcccgcgc ggcggcacgc acgcaggccg
       61 ccacgtcggc cgtgctgccc ggacgcagga ccgcaatggc gtcgccgggg aaaaggccgc
      121 gcatctcgtg ttcataaggc gcgcggtcgc ccggctcggt cagcacgtgc tccggcccga
      181 tcaggccctt gaactggtcg atcagtgcgg catggagggg aagcggcgtg gtcatgcctg
      241 gcatttgaac cgcacgccgt gccgcgtcaa cgtcccttgg acttgcgcca ggcggcgaaa
      301 tcgactttcg actgctcgtc cgtcggcgga taaaggccga ggatggaccg cccctccagc
      361 actttatcgg tgacgaaatc ctcgaacgcg gtcatctcga ccgcctcgtc ggcgatctcg
      421 tcggcgattt ccgccggaat caccaccaca ccctcgccat cgccgaccag cacgtcgccg
      481 ggaaacaccg ccacatcgcc gcagccgatc ggcacgttga tgtcgagcgc ctcgtgcagc
      541 gtcaggttgg tcggggcgga ggggcggctg tgataggcgg gaatggcaag ccgcgcgatc
      601 tccggcgcgt cgcggaagcc gccatcggtg acgacgccgg ccacgccccg caccatcagc
      661 cgcgaaacga ggatcgatcc tgctgaagcg gcgcgggcat ccttgcggct gtcgaacacc
      721 atgaccgcgc ccggggggca ttcctcaatg gccttgcgct gaggatgcgc gcggttctgg
      781 aacaccgtga tcggattgcg gtcctcgcgc gccggaatgt agcgcagcgt gaacgcctcg
      841 cccaccatcg tgggcaggtc gggatttagc gggcgcacgt cctggatgaa ctggttgcgc
      901 agtccgcgct tgaaaagcgc cgtgcaaagc gtcgcggtgg aaacggtctt cagtctgtcg
      961 cgcgtcgcgg gcttgaggcc ggacatgatc atgctccttc acggctaggg cgccgtcgag
     1021 cacttgtatg atgacctgat gagacgtcaa gccgcttgca tgcgtgaccg tgccgtccgc
     1081 acgcggggcg ccgcacacga gacctgcgcc cgtccggtgt atcatgcagg gatttcgctg
     1141 aaggtgccaa gactatcgcg tttcgatcca gcaatacggc cccgtgcgcc aacgcacaaa
     1201 cgcccgagtt ccgactgtta tgctgcattc atcgaaaccc ctttacgaag gattgaccat
     1261 gcctctcttc gacacctccg agatcatcat caccaatccg cagaatattc ccagcctgcc
     1321 gagcaatgca gagtacggga caagcggcaa tgactacatt accctcagcc agccgaagct
     1381 ggtctttgcc ggcgctggca acgatatcgt cttcggctcg accggcgccg atacggtcta
     1441 tggcggcctc ggcgacgaca tcattgttgg cggcgccggt gacgacaagc tctacggcga
     1501 cagcggcaac gacaggctct atgccggcac cgggtccgac agcatgtatg gcggcgcggg
     1561 caacgaccgg ctctatgtcg atcccaacga caacaccggc gccctggtcg acttggccaa
     1621 tggcggcagt ggcatcgaca ccctcgtgat caccaatgcg acagccggat ccctgcgcct
     1681 gttctcggtc gagggcattg ctggagcgat cgtccactgg accgagacgg ttggtggcca
     1741 gaccattgcc cagtcgatca ccgtcgaggg cgtcgagttc gtcgagttca acggcgcggc
     1801 catcgccctc cagaacatcc agccctactg atcggccgcg cgcgggtcat ctcccgcccc
     1861 gccctgacgt gactgtgccc gcccctcggg gcgggcattt tcgcagcgca acatgttaca
     1921 aaaatacttg acacataaag tttctgtaac atatagccgt atgcaggtcg cgggccggtc
     1981 cgcgcgaggg gaaacgctca tgtacagcca ggcgctcaac gtcgccgatc tcggcggcaa
     2041 tgaggaagac gaaggtgcgc tcgcccgctt ccaggtgcgg atcgacgccg aggacaagat
     2101 cgaacccaac gactggatgc cggagaaata tcggcgcacc ctggttcggc agatttcgca
     2161 gcatgcccat tccgagatcg tcggcatgct tcccgagggc aactggatca cccgtgcgcc
     2221 gagcctgaag cgcaaggccg cgctgctcgc caaggtgcaa gacgagggtg gccacggtct
     2281 ctatctctat tccgccgccg agacgctcgg tgtctcgcgg gaggagctgg tggaacagct
     2341 ccattccggc aaggcgaaat attcctcgat cttcaactat cccgccattt cctgggccga
     2401 catgggcatg atcggctggc tcgtggacgg ggccgcgatc atgaaccaga tcgcgctgtg
     2461 ccgctgttcc tacggtccct actcccgtgc gatgattcgc atctgcaagg aggagagctt
     2521 ccaccagcgc cagggctatg aaatcgtcac cacgctgatg cgcggcagcc agaagcagcg
     2581 ggacatggcg caggactctc tcaaccgctg gtggtggccg gcgctgatga tgttcggtcc
     2641 gtccgacaag gacagcatgc actccgattc ctccatgcac tggaagatca agcgcttctc
     2701 caacgacaac ctgcgccaga agttcgtgga cgcgacggtg ccgcagggcc actacctcgg
     2761 tctgaagttc ccggacccca agctgaggct ggacgaaaag acggggcact gggaattcgg
     2821 cgagatcgac tgggccgagt tcaagcgggt gatttccggc gacgggccct gcaaccgcca
     2881 gcgcatgaag gatcgggtcg cggcgcacga gagcggtgcc tgggtgcgcg cggcggcgga
     2941 agcccatgcg gaaaagcgcg cggcgcgcag gaatgcagca cggatggcag cggagtgacg
     3001 atcatgagca acaagaactg gccgctttgg gaagtcttca tccgcgcgcg caacggcatg
     3061 gcgcaccgcc attgcggctc gctccacgcg gtcgatgccg agatggcgct gcagaacgcc
     3121 cgcgactgct acacccggcg cggcgagggc acctccatct gggtggtgcc gtcgaacgcc
     3181 atcaccgcct ccgacccggc ggagaaggac atgatgttcg agccgaccgc ctcgaaggtc
     3241 taccggcatc ccaccttcta cgaggtgccg gaagaagtcg ggcacatgtg aggccggcga
     3301 tgtccacccc cctgttcgac tatacgatcc gccttgccga tacggcgctc gtcctcggcc
     3361 accgcctttc ggaatggtgc ggccacgcgc caatcctcga agaggacatg gcgcttgcca
     3421 atctggcact ggatctgatc ggccagtccc gctcgttcta cgattatgcc ggagccgtcg
     3481 agggcaaggg ccgcgacgag aatgcgctcg cctatctccg gctcgagccg gagtggaaga
     3541 atttgcttat cgtcgaacag ccgaacggcg atttcgccgc caccatcctg cgccagttcc
     3601 tggtcgcggc cttcctgcac ccgttctacc ggaggctgtc cggctcgaag gacgcgacgc
     3661 tggccgccat cgccgccaag gccgagaagg aaatggctta ccacgtgcgc cattgcggcg
     3721 agtgggtgat ccggctcggc gacggcacgg cggaaagcca ccggcgcatt tccgaggcgc
     3781 tggccgacct ctggcccttc accggcgaga tgttcgaggt cgatgccggc gatcgcgccc
     3841 tgatcgagca gggcatcgcc gtcgatgcgg tggccctgcg cgcggaatgg gagcagcttg
     3901 tcgaccgcgt cttcgccgag gcgacgctgg agcgtccggg cgacagctgg atgcagacgg
     3961 gcgggcgcaa cggccggcac accgaacatc tcgggcacct gctcgccgag ctgcagtaca
     4021 tgcagcgcgc ccatcccggc ctgacctggt gagttccatg gcgaccgcga tggatgccgt
     4081 ttcgccggac ttcctgcgcc gcaaggcctg gaccgcggcc gcgacggtgg tcgatccgga
     4141 agtgccggtg ctctccatcg ccgatctcgg cgtcctgcgg gatgtcacct tcgagggcgg
     4201 aaccgtggtc gccaccatca cgccgaccta ttccgggtgc ccggcgatga atctcatcgc
     4261 ccttgaggtt gatctggcgc tcgagaaggc tggctttacg cgccgccgtg tcgatctcaa
     4321 gctggcgccg gcctggacca cggactggat gagcgccgag ggtcgcgaga agctgcgggc
     4381 ctatggcatc gccccgccga ggccgcgttc cgcctcgggg cgtggcgcgc tgtttggcgt
     4441 tgaggacatc gcctgtccga aatgcggctc ggacaacacc gagcgcatca gcgaattcgg
     4501 ttccacggcc tgcaaggcgc tgtggcgctg caaggcctgc cgcgagccgt tcgattactt
     4561 caaatgcatc tagggtgaaa actcatgagc cgctttaacg ctctcaccgt gtccgacatc
     4621 cgccgcgaga cgccgcaggc cgtgtccatc gccttccgcg tgccggcgga cctcagcggc
     4681 gactaccgct tcgagcatgg ccagtatctg acgctgcgcc gggatatcgg cggccaggat
     4741 gtgcgccggt cctattcgat ctgcaccgga accgaggacg gcgaattgcg catcgccgtg
     4801 aaggaggtgg aaggcggcct gttctccacc ttcgtcaacc gcgaccttgc ggttggcgat
     4861 accctcgagg tgatgacgcc gatgggccgg ttcaccgcgc cgatcgagcc ggaagcggca
     4921 aagacctatg tcggcatcgc cgcaggttcc ggcatcacgc cgctcatgtc gatcatccgc
     4981 accgtgctcc agcgcgagcc gaaaagccag ttcgtgctgc tctacggcaa ccgaacggcg
     5041 aattcggtga tcttcaagac cgaactggaa gacctgaagg accgattcct cagccgcctc
     5101 acggtctatc atgtcctgtc gcgcgaggcg caggacgtgg acctgttgca cggccggctt
     5161 gaaggcacgc gcatctcccg cctggtgcgc tccgccctcg gcgatgaggc catcgaccat
     5221 gccttcatct gcggcccgga ggccatgacc gagtcggcgc ggcaggctct cgtcaacctc
     5281 ggcgtccagg ccgagcgcat ccacaccgag ctgttcacca cgaccacgcc ggcccggccg
     5341 cgcccgtcgg tcgcgccgaa ggcgggcgac gaaatcgcgc tgaccgtgac cttcgacggt
     5401 gccacgcatg cgctgaccat cggacctgac gaaacggtga tcgacgccgc agcgcgccag
     5461 ggcgtggacc tgccctattc ctgcaagggc ggcatgtgct gcacgtgccg cgccaaactc
     5521 accgagggcg aagtgaagat ggaccaccag ttctcgctgg agccctggga gctcgaagcc
     5581 ggctatgtgc tgacctgcca gtcgcggccg gtgaagggtc cggtcgcgct ggactacgat
     5641 cacgcctgag gatcgacata ggaccatgat cctaattttc ttgacagccg gaccattccg
     5701 tggtgtcctg ccgtcattcc cgccgcgaca ggcgtgtggc cacagccatc cgggcgggaa
     5761 cggcagccgg ttgcacggcg cggtcgaaag ccgcgcaatg gaccggaaac ggcgggtcgg
     5821 aaccctgaaa aggggccggg ccagccatgc cgggcgcggg cgggaccgga gcctgtcctg
     5881 accacccaat gcctacccca atgctcgaag cgcttcgagg gaccgaaccc cggcgctccg
     5941 tccgcgcccc ctgtcgccct ttcgtcgccc gcgcgcctaa ggccgcgtcg gtgcgccctt
     6001 gatgatgctg actcgcccat cgcgcatcaa tgacgagcgc cgtgggactt cgcgattcca
     6061 tcgcctgtca ctggaaacgg caggtcacgg ccagaaccat cgtcctggcc cttggcctca
     6121 gatcgtcagc gttccggcct tggcggcggc gtagcgttcg cccaccttgg tccagttgac
     6181 cacgttccac caggtggcga gatactcgtt ccgccggttc tggtacttca ggtagtaggc
     6241 gtgctcccac acgtcgttgc cgaacagcac gcgctggccg tccatcagcg gggaatcctg
     6301 gttcgggcgg gtggtgagcg ccagcttgcc gtccttgtcg acggtgacga atacccagcc
     6361 cgaaccgaac accctgaggc ccgagccgtt gaactgctcc ttcagcttgt cgaagccgcc
     6421 gaaagccgcg tcgatcgccg ccttgacctc gccgcctggc tctttcgcgc cgccgggcgc
     6481 catgacctgc cagaacatcg tgtggttggc gtggccgccg aggttgttct tcatgccgac
     6541 acgcgcggcc tccggcagct gctgccagcc ggcgagcagt gtctcgaccg gcgtggtgcc
     6601 gagcgcgccg tgatccttgg cgaacgtgtt gaggttgccg atgaaggcgc cgtggtggcg
     6661 caggaaatgg gtctccatgg tcttggcgtc gatatgcggc tcgagcgcgt cgaaggcgta
     6721 gccgagcggc gcctgaacga acgggccggc cggggcctgg gcctgggcgc gaccgaagct
     6781 gacggccgtg gcggccgcgc caagcaggaa catgcggcga gtggcgtgca tttttcgtga
     6841 gctccctcta tccgccggca ttctgctgta taggggcgac ggtccctgat gataggggag
     6901 attgggcgag agtttgaccc cgtgcaagtg aaatgatcca tctgcgcgat cacgtacagg
     6961 agggccagcc atgatccggc gcatgcggtc cgacaggctc ctcgttgaac gcggtgtgtt
     7021 cgacagccgg gcgcgcgcgc aggctgcgat cgccgccggc ctcgtgcggg
//
LOCUS       DAOMXN010000138         7048 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_5595_length_7048_cov_11.341627, whole genome shotgun sequence.
ACCESSION   DAOMXN010000138 DAOMXN010000000
VERSION     DAOMXN010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 7048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7048
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5595_length_7048_cov_11.341627"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PK812_09800"
     CDS             complement(<1..183)
                     /locus_tag="PK812_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein dehydratase"
                     /protein_id="HRJ69892.1"
                     /translation="MRTEASLDVAHLSRWIGREETVVDGVGEDLARKFHAMLALPGPV
                     PKAGDPAPRIIHFCLGQ"
     gene            complement(180..1283)
                     /locus_tag="PK812_09805"
     CDS             complement(180..1283)
                     /locus_tag="PK812_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HRJ69893.1"
                     /translation="MPGRPPLEGLTVVAVEQAVAAPFCTSRLADAGARVIKIERPEGD
                     FARGYDAVAKGQSSYFVWLNRGKVSRVLDLADERGKAELAGLIASADVLVQNLKTGAL
                     ARLGFAPEELRRKHPRLIVCSISGYGDSGPMAERKAYDLLIQAESGLCSITGGPSGPA
                     RVGISVVDIATGATAHAAILEALIRRGITGEGADIRIAMFDVMADWLAVPLLNHEGGR
                     TPTRTGLAHPSIAPYGVFATRDGRQVLISIQSEREWAQFCAEFLQDASISKDPRFSSN
                     VARVNHRAETDGMVARAFADLDLDEAVARLTRADTAFAAVSDMAALARHPHLRRITVD
                     TPNGPVAYPAPAPIFVGETRAYDKVPAIGACDP"
     gene            complement(1283..2446)
                     /locus_tag="PK812_09810"
     CDS             complement(1283..2446)
                     /locus_tag="PK812_09810"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRJ69894.1"
                     /translation="MQTVGSDRFEDIRQAVRALCAEFPAEYHRKVDAARGYPEAFVEA
                     LTRAGWMAALIPEEYGGAGLGLTEASVIMEEINRAGGNSGACHGQMYNMGTLIRHGSE
                     AQRRNYLPKIAAGDLRLQSMGVTEPTTGTDTTRIKTTAVRQGDRYVINGQKVWISRIQ
                     HSDLMILLARTTPLDQVKKKSEGLSIFIVDLAEAQKTGMTVRPIPNMVNHETNELFFD
                     NLEIPAENLIGEEGQGFRYILTGLNAERTLIAAECIGDGYWFIDKVTAYASERVVFGR
                     PIGQNQGVQFPIAESFIEVEAANLMRYRACELYDAMKPCGAEANMAKYLAAKASWEAA
                     NACIQFHGGFGFACEYDVERKFRETRLYQVAPISTNLILSYVAEHILGLPRSF"
     gene            complement(2535..4361)
                     /gene="typA"
                     /locus_tag="PK812_09815"
     CDS             complement(2535..4361)
                     /gene="typA"
                     /locus_tag="PK812_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012453136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HRJ69895.1"
                     /translation="MSLRNIAIIAHVDHGKTTLVDKLLQQSGAFRENQRIVERVMDSN
                     DLEKERGITILAKCTSVVWKDTRINIVDTPGHADFGGEVERILNMVDGVIVLVDAAEG
                     PMPQTKFVVSKALKLGLRPIVAVNKVDRSDARATEVINEVFDLFAALDATDEQLDFPI
                     LYGSGRGGWMATSPEGPQDQGLAPLFDLVLAHVPVARTEDGSFRMIGTILEANPFLGR
                     IITGRVTSGSIKPNQPVKVLSRDGQLIEQGRVTKILAFRGIERTAIDEAVAGDIVSIA
                     GLTKGTVADTYCALDVSEPLPAQPIDPPTVSMSFIVNDSPLAGTEGDKVTSRVIRDRL
                     MREAEGNVALKIEESADKDSFIVSGRGELQLAILIETMRREGFELAVSRPRVVFQSND
                     AGQTLEPVEEVFIDVDETHSGVVVQKMSERKAEMLEMKPSGGNRVRLVFHAPTRGLIG
                     YQSELLTDTRGTAIMNRLFHSYQAYKGEVEGRRNGVLISNDIGEAVAYALWNLEDRGP
                     MVIEPGWKVYRGMIVGIHTRDNDLEVNVLKGKKLTNIRAAGKDEAVRLTPPVRMTLEK
                     ALAWIQDDELVEVTPKSIRLRKRLLDANDRKREEKSREALAG"
     gene            complement(4460..5770)
                     /locus_tag="PK812_09820"
     CDS             complement(4460..5770)
                     /locus_tag="PK812_09820"
                     /inference="COORDINATES: protein motif:HMM:NF028178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRJ69896.1"
                     /translation="MRRSVVLLMIAGILALTALGALAIYLFAAPTPLRIAVGPVGSED
                     TRLVAAILQTFAREKHPIRLRMVPTDGPSGAAEQLASEKVDLAIVRSDGNVPPNAATV
                     AIMHRDVAVILATPASRITNIGDLAGKRVGIIRGYNLNQKFLEFVLERSGVDRTSVAK
                     VNLRAQDVRASVAEGKIDAILAVAPVTANFLSTLQQDLLTALGQYPVIVPIADAEAIA
                     QRNPLFETFTILRGTFGGSPPRPSENIPTLAVTHRLVADRKLKDRVVADLAKSLFEIR
                     QVVAVDAPIGARIEQPNSESPGPLVTHPGAAAYFDGEQRTFIEQYGEWIYIAVMGVSL
                     IGSLGAALLSRFLGSSRPTGQSDLDRMLLLLRRTRSAMSIADLDEVQREADESFTRMV
                     ERSARSEIEEAVMSAFTIALGEVRNAIEDRRRSIASEELGSLAE"
     gene            5979..>7048
                     /locus_tag="PK812_09825"
     CDS             5979..>7048
                     /locus_tag="PK812_09825"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HRJ69897.1"
                     /translation="MSSKPSLFRALLWPIPLIVGIVGVALFYQVQHFVENRAVEEAVA
                     ETNSRLDTLRKIRSFYNENVVRKVVAGSSLRTATNFRASEDAIPYPATFLHEIAAIDS
                     LDGRSHGLVSPFPFANRADRQMKAWEREAWDRLKAEPDARVVLPVTTEAGAFLYVAVA
                     DKLGAQTCVDCHNSHPDSLKKDWHLGDMRAVFGSLVPLGSIFARANEMRNTIFLTLSV
                     GLAFGIIIYLILVRIAHRRLIGAVEMLRRVVTGDEASVPATGARHTETARLQEAAIAF
                     QEAQRRRRELESERLAKARESADRARLITQGTDAFQTATERTFQRLNALGGGLIDKAT
                     SLDAAARQLGERTQSAVAAATAT"
BASE COUNT         1224 a         2301 c         2258 g         1265 t
ORIGIN      
        1 ctggccgagg cagaagtgga tgatgcgcgg cgcaggatcg cccgccttcg gcaccggccc
       61 gggcagtgcc agcatggcgt ggaatttccg cgccagatcc tcgccgacac catcgacgac
      121 cgtttcctca cgtccgatcc accggctgag gtgggcgaca tccagcgacg cttcggtcct
      181 catgggtcac acgctccaat cgccggcacc ttgtcgtagg cgcgggtctc gcccacaaag
      241 atcggcgcgg gggcgggata ggcgaccgga ccgttcggcg tgtcgacggt gatgcgcctg
      301 aggtgcggat ggcgcgccag cgccgccatg tcactgaccg cggcaaaggc cgtgtccgct
      361 cgggtcaggc gcgccaccgc ctcgtcaagg tccaggtccg cgaaggccct ggcgaccatg
      421 ccgtcggtct ccgcgcggtg gttcacgcgt gcaacattcg aagaaaagcg cggatccttg
      481 cttatggacg catcctgcag gaactcggcg cagaactggg cccattcccg ctcggactgg
      541 atggaaatga gcacctgccg gccatcgcgc gtcgcgaaca cgccataagg cgcgatggag
      601 ggatgggcga gccctgtccg cgtcggggtc cggccgcctt catggttcag gagcggcacg
      661 gcgagccaat ccgccatgac atcgaacatc gcgatgcgga tgtcggcgcc ctcgccggtg
      721 atgccgcgcc ggatcagcgc ttccaggatc gcggcatgcg ccgtggcgcc tgtcgcgata
      781 tccacgacgg aaatgccgac gcgggccggc ccggatggtc cgcccgtgat ggaacacaag
      841 ccggattcgg cctggatcag cagatcatag gccttgcgtt cggccatcgg gccgctgtcg
      901 ccatagccgg agatcgagca gacgatcagg cgcggatgtt tccgcctcag ttcttccggc
      961 gcaaagccga ggcgggcaag cgcgcccgtc ttgaggttct gcacgagcac gtccgccgac
     1021 gcgatcaggc cggcaagctc ggccttcccc cgttcgtcgg cgagatcgag aacgcgcgac
     1081 accttgcccc ggttcagcca gacgaaatag ctcgactggc ccttggccac ggcatcgtag
     1141 ccacgcgcga aatccccctc ggggcgctcg atcttgatca cccgcgcgcc ggcatccgcc
     1201 agccgcgaag tgcagaacgg agccgccacc gcctgctcca cggcgacgac ggtcaggcct
     1261 tcgagcggcg gacggcccgg catcagaacg acctcggcag gccgaggatg tgctcggcga
     1321 cataggacag gatgaggttc gtggagatcg gcgcgacctg atagaggcgc gtctcgcgga
     1381 acttgcgctc cacatcatat tcgcaggcga acccgaaacc gccgtggaac tggatgcagg
     1441 cgttggcggc ttcccatgag gccttcgccg ccagatactt ggccatgttc gcctcggcgc
     1501 cgcagggctt catggcatcg taaagctcgc aggcccggta gcgcatcagg ttggctgctt
     1561 ccacctcgat gaagctctcg gcgatgggaa actgcacgcc ctgattctgg ccgatcggcc
     1621 ggccgaacac cacgcgctca ctcgcgtagg ccgtcacctt gtcgatgaac cagtagccgt
     1681 cgccgatgca ttccgccgcg atcagggtcc gctcggcatt gaggccggtg agaatgtatc
     1741 tgaagccctg cccctcctcg ccgatcagat tctcggcggg aatttcgaga ttgtcgaaga
     1801 agagttcgtt cgtctcgtga ttgaccatgt tgggtatggg gcggacggtc attccggtct
     1861 tctgcgcctc ggcgaggtca acgatgaaga tcgacaatcc ctcggacttc ttctttacct
     1921 gatcgagcgg cgtggtgcgg gccagcagga tcatcaggtc cgagtgctgg atgcgggaga
     1981 tccagacctt ctggccgttg atgacgtagc ggtcgccttg tctgaccgcc gtcgtcttga
     2041 tcctcgtcgt atcggtgccg gtcgtcggct cggtcacgcc catggattgc aggcgcaagt
     2101 cgccggcggc gatcttcgga aggtagttcc ggcgctgcgc ttccgatccg tgccggatca
     2161 gcgtgcccat gttgtacatc tggccgtggc aggctccgga attgccgccc gcgcggttga
     2221 tttcctccat gatgacgctc gcctcggtca ggcccagacc cgcccccccg tactcttccg
     2281 ggatcagcgc cgccatccag ccggcccgtg tcagcgcctc gacgaacgct tcgggatagc
     2341 cgcgcgccgc gtcgaccttg cgatggtact cggcgggaaa ctcggcgcag agcgcccgca
     2401 cggcctgacg gatgtcttcg aacctgtcgc tgccaacagt ctgcatgtgt gtctcgaccg
     2461 atcagatact catcccttcc gggaccgccc gatctgtctc atgagcacgc gcgcccgaca
     2521 acccggatgt cggcctatcc cgccagggcc tcgcggctct tctcttcacg cttgcgatcg
     2581 ttggcgtcga gcaggcgctt gcgcaggcgg atcgacttcg gcgtgacctc gaccagttcg
     2641 tcatcctgga tccaggccag cgccttttcc agcgtcatgc gcaccggcgg cgtgaggcgc
     2701 acggcctcgt ccttgccggc ggcgcggatg ttggtcagct tcttgccctt gaggacgttc
     2761 acctcgagat cattgtcgcg ggtgtggatg ccgacgatca tgccgcggta gaccttccag
     2821 cccggctcga tcaccatcgg gccgcgatcc tcgaggttcc acaaggcata ggccacggcc
     2881 tcgccgatgt cgttggagat gaggacgccg ttgcggcggc cttccacctc gcccttgtag
     2941 gcctgatagg agtggaacag ccgattcatg atcgcggtgc cacgggtatc ggtcagcaat
     3001 tccgactggt agccgatcag gccgcgggtc ggcgcgtgga agacgagacg cacgcggttg
     3061 ccgccagagg gcttcatctc cagcatttca gccttgcgct ccgacatctt ctgcacgacc
     3121 acgccggaat gcgtttcgtc gacgtcgatg aagacttcct cgaccggctc cagcgtctgc
     3181 ccggcgtcat tcgactgaaa cacgacgcgc ggccgggaga cggccagttc gaagccctcg
     3241 cggcgcatgg tctcgatcag gatggccaac tggagttcgc cgcgaccgga gacgatgaag
     3301 ctgtccttgt cagccgattc ttcgatcttc agcgcgacat tgccttccgc ctcccgcatc
     3361 aggcgatcgc ggatgacgcg gctcgtcacc ttgtctccct cggttccagc caggggagaa
     3421 tcgttgacga tgaaggacat cgacaccgtc ggagggtcga tcggctgggc cggcaacggc
     3481 tcgctcacgt ccagcgcgca ataggtgtcg gccaccgtgc ccttggtcag gccggcgatg
     3541 gaaacgatgt cgccggcgac ggcctcgtcg atggccgtgc gctcaatgcc gcgaaaggcc
     3601 agaatcttgg tcacccgccc ctgctcgatg agctgaccat cgcgcgacag caccttgacc
     3661 ggctgattcg gcttgatcga acccgacgtg acgcggccgg tgatgatgcg accgaggaag
     3721 gggtttgctt ccaggatcgt gccgatcatg cggaaggagc catcctccgt acgggcgacc
     3781 ggcacgtgtg ccagcacgag atcgaacagc ggcgccaggc cctgatcctg cgggccttcc
     3841 ggcgaggtgg ccatccagcc gccgcggccg gagccgtaca ggatcgggaa atcgagctgt
     3901 tcgtccgtgg cgtcgagggc ggcgaagagg tcgaacacct cgttgatcac ctcggtcgca
     3961 cgggcgtcgg agcgatcgac cttgttgacc gcgacaatcg ggcgaaggcc cagcttcaat
     4021 gccttggaga cgacgaattt cgtctgcggc atcggccctt ccgccgcgtc caccagcacg
     4081 atcacaccat cgaccatgtt gaggatgcgc tcgacctcgc cgccgaaatc cgcgtgcccc
     4141 ggcgtatcca cgatattgat gcgcgtatcc ttccagacca ccgaggtgca tttcgccaga
     4201 atggtgatcc cacgttcctt ttccaggtcg ttggaatcca tgacgcgctc gacgatgcgc
     4261 tggttctcgc gaaaagcgcc ggattgctgc aacagcttgt ccaccagcgt tgtcttgccg
     4321 tggtcgacgt gggcgatgat ggcgatgttg cgcagtgaca tgggttttct ggcttccaac
     4381 agcgaaaagc gccggacgat cggtccggcg caatcggata ttgcgctgca atagcggttt
     4441 gatgacggct cggcaacctt tactcggcga ggctgccaag ttcctcgctg gcaatggagc
     4501 gacggcggtc ctcgatggcg ttgcgaactt cgccaagggc aatcgtgaag gcgctcatca
     4561 cggcctcctc gatctccgac cgggccgagc gttcgaccat gcgcgtgaaa ctctcgtccg
     4621 cctcgcgctg aacctcgtcc agatcggcga tcgacatggc cgagcgcgta cgtcgcagca
     4681 ggagcagcat gcgatccagg tcggactgcc ctgtcggccg gctgctgccg aggaaccggc
     4741 tcaacagggc ggcgccaagc gagccgatca ggctcacgcc catgacggcg atgtagatcc
     4801 attcgccgta ttgctcgatg aacgtgcgct gctcgccatc gaaataggcg gccgcgccgg
     4861 gatgcgtcac cagcggaccg ggcgattccg aattcggctg ctcgatccgc gcgccgatgg
     4921 gcgcgtccac cgcgacgacc tggcgaatct cgaacaggct cttggccagg tccgcgacca
     4981 ccctgtcctt gagcttccgg tccgcgacga ggcgatgcgt gacggcaagc gtggggatgt
     5041 tctcggaggg acgcgggggc gaaccgccga acgtcccgcg caggatcgtg aaggtctcaa
     5101 acaacggatt gcgctgggcg atcgcctccg catccgcgat cggaacgata acggggtact
     5161 ggccgagggc cgtcagcagg tcttgctgca gcgtcgacag gaaattcgcc gtgaccggcg
     5221 cgacggcgag gatggcgtca atctttccct cggcgactga cgcacgcacg tcctgtgcgc
     5281 gcagattgac cttggcgacg cttgtacggt cgacaccgct gcgttcaagc acgaactcca
     5341 ggaacttctg gttgagattg tagccacgga tgatgccgac acgcttgccc gcgagatcgc
     5401 cgatgttcgt gattctactg gcaggcgtcg ccaggatgac ggcaacgtcg cgatgcatga
     5461 tggcgacggt cgcggcattg ggtggcacat tgccgtcgct gcgaactatg gccaggtcaa
     5521 ccttctcact tgccagctgt tcggcggcgc cgctcggtcc gtcggtcgga accatgcgca
     5581 agcggatcgg atgcttctcg cgcgcgaagg tctgaaggat ggcggcgaca aggcgcgtat
     5641 cctcgctgcc aaccggccca accgcgatgc gcagcggcgt cggggcggcg aacagataga
     5701 tcgctagcgc tcccagcgcc gtcagcgcga gaatgcccgc gatcatcagg agtacgactg
     5761 atcgccgcac aacaggctcc gttttttcat catcaccgtt ccgcgtgtct atcgcgatgg
     5821 cgtcacgatg gccatgcggt ccgttggcgc ccagtgtcac aaaacggcga ccccggatgc
     5881 aacctttaat tgtgtgccca gaaaaatacc cgcatttaca gccgattaac catgttgatg
     5941 gacactgcgc ggcacatatc aatgcctcgg agatctcggt gtcatcgaag cccagcctgt
     6001 ttcgcgcgct gttgtggccg atcccgctca ttgtcggaat cgtcggggtg gcgctgttct
     6061 accaggtcca gcatttcgtg gagaaccgcg cggtcgaaga agccgtggcg gagacgaact
     6121 cgcgactgga cacgctgcgc aagatccgga gcttctacaa cgagaatgtc gtgcgcaagg
     6181 tcgtggccgg cagttcgctg cgcaccgcga ccaatttccg tgcgagcgaa gacgccatcc
     6241 cctatccggc gacattcctc catgagatcg ccgccatcga cagcttggac gggcgcagcc
     6301 atggcctggt cagccccttt cccttcgcca acagggccga tcggcagatg aaggcctggg
     6361 agcgcgaggc ttgggaccgg ttgaaagcgg agcccgatgc ccgcgtcgtg ctgcccgtca
     6421 ccacggaagc aggcgcgttt ctctacgtgg cggttgccga caagctcggt gcgcagacct
     6481 gcgtggattg ccacaactcc caccccgaca gcctgaagaa ggattggcat ctcggagaca
     6541 tgcgggcggt gttcggcagt ctcgtgcccc tgggatcgat cttcgcgcgt gcgaacgaaa
     6601 tgcggaacac gatctttctc acgctttcgg tcggtctcgc cttcggcatc atcatttatc
     6661 tcatcctggt ccgcatcgcg caccgccggt tgataggggc ggtggaaatg ctgcgacgcg
     6721 tcgtgaccgg agacgaggcg tccgttcccg ccaccggggc caggcacacg gaaaccgcac
     6781 gcctgcaaga ggcggccata gccttccagg aagcacagcg ccgccggcgc gaactggaga
     6841 gcgagcggct ggcaaaggcg cgagaaagcg ccgaccgggc gcgtctcatc acgcagggca
     6901 ccgacgcctt ccagaccgcg accgaacgca cgttccagcg cctcaacgcc cttggcggag
     6961 ggttgatcga caaggccacc tcactcgacg cggcggcccg ccagctgggc gagcgcacgc
     7021 aatcggcggt tgccgccgcc acggccac
//
LOCUS       DAOMXN010000139         7030 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_5610_length_7030_cov_9.345090, whole genome shotgun sequence.
ACCESSION   DAOMXN010000139 DAOMXN010000000
VERSION     DAOMXN010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 7030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7030
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5610_length_7030_cov_9.345090"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="PK812_09830"
     CDS             complement(<1..716)
                     /locus_tag="PK812_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009464931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose 1,6-diphosphate aldolase"
                     /protein_id="HRJ69898.1"
                     /translation="MTALSAGKLWGMRRMADAAGRFKMTAVDQRPPIINPIAKARGGS
                     APWEDVAAFKTMLIKELQAESTALLLDPLYAIPHGAAVMDPRKALIMTLEEHAFVDTP
                     EGRLSAEIDGWSVEKIKRSGGDAVKVLAWYRPDAGAGVNQSQKDFVKRVGEACARYDL
                     PYVFELLVYPLAGEANQTKDYVELAAKHADHVLESVRIFAAPDYGVDLFKLESPVPAA
                     GCDGSAKVQALFDEMGHLAGR"
     gene            complement(733..1626)
                     /locus_tag="PK812_09835"
     CDS             complement(733..1626)
                     /locus_tag="PK812_09835"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRJ69899.1"
                     /translation="MHAILLIGFSCQDFVFSVDDLPSKGEKYRARSLEVVGGGLSANA
                     AVAIARLGGRAILVTYLGDDGTGRDILAELAAEGVDCSLSRRFAGARSPLSAIAVDKR
                     GERMILNYADLSIPPDIAWLPERLPPGVCAVQGDSRWEPGSIHAFRLARGAGVPSVLD
                     ADRAVRSPDILTAPTHVAWSAQGLREMTGANDLSAAVAAESRRRGNWVAVTDGENGCW
                     FARDGRVEHEPAFAVDVVDTLGAGDTFHGALTLALGQGMPERRAVRFASAAAALKCTR
                     FGGRKGVPAQDEVERLVAGRN"
     gene            complement(1716..>3587)
                     /locus_tag="PK812_09840"
     CDS             complement(1716..>3587)
                     /locus_tag="PK812_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009449902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRJ69900.1"
                     /translation="IVATLTVVAALLPMLFVSGLMGPYMSPIPSNASAAMVFSFFVAV
                     MVTPWLMVKLGGGAHGGGQGGHHDEGWLGRMYRKSARPVLATKPRAWTFLILTGVLTL
                     GSLALFYTQSVTVKLLPFDDKPELAVMLDLPRGASVEDTDRALQDAAHALRGVKDVDT
                     VQAHAGTAAPFNFNGLVRHTYLRSETWMGDLQLNLVSRDKRSRPSHDVALEVRERLKA
                     ATLPQGTVVKVVEPPPGPPVLATLLAEIYGPDGETRRQVAEKVRSAFKSVPFIVDVDD
                     TLALQPQRRRLTVVDEQLEFHKVEQRDVFDTIRLLYGGVTVGYSHRGGGRQPIPIRVA
                     LEKSGRLLDERALSVPIPANVLPGARGVVELGDVVRIENEPPSPQIFRRNSRPAEMVM
                     AELAGAFEAPLYGMIAVQRALDAQDWGTLPKPRIILYGQPDDTAKPSLLWEGEWEVTW
                     VTFRDMGAAFAVAMLGIYILVVAQFGSFKAPLVILTPVPLTFIGIMFGHWLFGAPFSA
                     TSMIGFIALAGIIVRNSILLVDFIRHARSPDIPLLETLIDAGAIRFKPILLTALAAMI
                     GAATILTDPIFQGLAISLLFGLASSTLLTLLVIPAAYVVLRTPATTVEAAETRQGEA"
     assembly_gap    3588..3687
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3688..4766)
                     /locus_tag="PK812_09845"
     CDS             complement(<3688..4766)
                     /locus_tag="PK812_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009449902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRJ69901.1"
                     /translation="MTRPPLGIAGALTRAFIASPLTPLFLIVALALGMVALVALPREE
                     EPQISVPMVDIHVRADGLKAEDAVKLVTEPLEAIVKSINSVEHVYSQTSDHGAVVTAR
                     FVVGTSSDAAILRVHEKLRANLDRIPAGIPEPLVVGRGIDDVAIVVLTLAPKPEAAER
                     WSATALTRLARELRIEIAKLQDVGLTYLVGEQSDEIRIVPDPEKLARYGLTLQALVAK
                     VESANRAFPAGSIREGGQQITLIAGRTLAEIEDIANISVTTRDDRPVYLRDVASVRLA
                     GDPAERMVWHLQRDAQGAFVRTPAVSIAIAKRAGANAVTIAQQIITAVERARGGIVPA
                     DVTVHVTRNYGETANHKANELLFHLG"
     gene            complement(4763..>5542)
                     /locus_tag="PK812_09850"
     CDS             complement(4763..>5542)
                     /locus_tag="PK812_09850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRJ69902.1"
                     /translation="AVKQGERIALVADDKLALQLNAAEARIRALTTEQANARTEYDRA
                     RDLLSRGAGTQQRVDQTRTAYEVFRNQIAAAEADKAVIMQQSAEGAVVAPATGRVLSR
                     AATRGSVILPGEPVATIAGGGFFLRLALPERFAATLKVNATVPVDGRPGAAASSGRLV
                     KIFPQLENGKVIADVEVEALGDFFVGERVLVHVPVATRKALAVPSSAIAHRAGVDFVA
                     LATPNGRRDIVIVAGGPVATPQGPGVEVLSGLKAGDRVIVP"
     assembly_gap    5543..5642
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5643..5824)
                     /locus_tag="PK812_09855"
     CDS             complement(<5643..5824)
                     /locus_tag="PK812_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HRJ69903.1"
                     /translation="MCIDKAVMAFAGVMILLSVVLTQLVSPWFWLFTVFIGLNMLQSA
                     FTGFCPAAMIFKKLGAR"
     gene            5948..6301
                     /locus_tag="PK812_09860"
     CDS             5948..6301
                     /locus_tag="PK812_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69904.1"
                     /translation="MQVVCTRHAVSNGDDVEAPHRRSFDFPDGTELPDVLTAIARSGW
                     LPGIAGGRATWSVASGRLVAVIAQEWQAPKFLPIIEAPSLDWRDGALFLHFNYHAQID
                     PETVYRIFWGMRLQA"
     gene            complement(6368..>7030)
                     /locus_tag="PK812_09865"
     CDS             complement(6368..>7030)
                     /locus_tag="PK812_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family substrate-binding domain-containing
                     protein"
                     /protein_id="HRJ69905.1"
                     /translation="QARDRAMLLRIGAIDSAAAGLLPLLLQDLRAAHPGLEVRMVEDK
                     TIRLLPRLLSGRLDLAFLRPPETRDPRLVFRHLFFETAVVALHEGHALARGDAITVED
                     LRGQPMIVPDRRARPHSHDLTVKLFADAGFGARVAQVADEKQTIVNLVAAGIGLAIVP
                     RWAARLAVPGVVFRPLADASQATRSRLPLSAAYLRSTRDSARDAALDVVASRLAAYAA
                     QA"
BASE COUNT         1214 a         2309 c         2226 g         1081 t
ORIGIN      
        1 cggccggcca gatggcccat ctcgtcgaac agggcctgca ccttggcgga accgtcgcag
       61 cctgcggccg gcaccggact ttccagcttg aacaggtcga ccccgtagtc cggcgctgca
      121 aagattcgca cgctttccag cacgtggtcg gcgtgcttgg ctgccagttc cacataatcc
      181 ttggtctggt tggcctcgcc agcaagagga tagaccagca gctcgaagac atagggaagg
      241 tcgtaacggg cgcaggcctc gcccacccgc ttgacgaagt ccttctggct ctggttgacg
      301 ccggccccgg catccggccg gtaccaggcc agaaccttga ccgcatcgcc gcccgagcgc
      361 ttgatcttct ccacggacca gccatcgatc tccgccgaca aacgcccctc cggcgtatcg
      421 acgaaagcat gctcctccag cgtcatgatg agcgccttgc gcgggtccat cactgccgcg
      481 ccgtgcggaa tcgcatacag gggatcgagc agcagagcgg tggattccgc ctgcaattcc
      541 ttgatcagca tagtcttgaa cgcggcgacg tcctcccagg gtgcgctccc gccccgtgcc
      601 ttggcgatcg gattgatgat cggcggacgc tggtccaccg ccgtcatctt gaagcgcccg
      661 gcggcatccg ccatgcgccg catgccccag agttttcccg ccgatagcgc ggtcatgcct
      721 agctcctgtc tgtcagttcc gtccggcgac gaggcgctcg acctcatcct gtgcgggtac
      781 gcccttgcgc ccgccaaacc gtgtgcattt caacgccgcg gccgccgatg cgaagcgcac
      841 ggcacgccgt tccggcatgc cctggccgag cgccagcgtc agcgcgccat ggaacgtgtc
      901 gcccgctcca agcgtgtcca ccacatcgac cgcaaaggcc ggctcgtgct cgacacgccc
      961 gtcccgggcg aaccagcagc cgttctcccc atcggtgacc gccacccaat tgccgcgacg
     1021 tcgggattcc gccgcgacgg cggctgaaag gtcgttcgcg ccggtcattt cccgaagccc
     1081 ctgcgccgac caggcgacat gggtgggcgc ggtcaggatg tccgggctgc ggaccgcacg
     1141 gtccgcatcg agcacactgg gcacgccggc tccgcgcgcc agcctgaagg catggatcga
     1201 gccgggctcc cagcggctgt cgccctgcac ggcgcagacg ccgggcggca ggcgttccgg
     1261 caaccaggct atgtcgggcg ggatcgaaag gtcggcgtaa ttcaggatca tccgctcgcc
     1321 gcgcttgtcc acggcgatgg cggaaagggg cgagcgagcg cccgcgaagc gtctggacag
     1381 tgaacagtcc accccttcgg ccgcaagttc cgccaggatg tcgcgtcccg taccgtcatc
     1441 cccgaggtac gtgaccagga tggccctgcc cccgaggcgc gcgatcgcca ccgccgcatt
     1501 ggcggacagg ccgccgccca ccacctcaag cgagcgggcg cggtacttct cccccttcga
     1561 cggcaggtcg tcgacggaga aaacaaagtc ctggcaggaa aatccgatca acaggatagc
     1621 gtgcatggtc gctgtgccgc agggaagtca gccccgacgg tatcaggtct tatgtctttt
     1681 acaagactct gtgcagagct ccctggaccg cgccgctatg cctccccctg tcgcgtttcc
     1741 gccgcctcga cagtcgttgc cggcgtccgc agcaccacat aggccgccgg gatcaccagc
     1801 agcgtcagca acgtagagga ggcgaggccg aacaacagcg aaatcgccag tccctggaag
     1861 atcgggtcgg ttaggatcgt cgcggcgccg atcatcgcgg cgagcgcggt gagcaggatc
     1921 ggcttgaaac ggattgcgcc ggcatcgatc agcgtctcca agagcgggat gtccgggctt
     1981 ctggcatgcc ggatgaagtc gacaagcaga atcgagttcc ggacaatgat gccggcaagc
     2041 gcgatgaagc cgatcatcga cgtcgccgag aacggggcgc cgaacagcca gtgcccgaac
     2101 atgatgccga tgaaggtcag cggtaccggg gtcaggatca ccagcggagc cttgaaggac
     2161 ccgaactggg cgacgacgag gatgtagatg cccagcatcg ccacggcaaa cgccgcgccc
     2221 atatcgcgga atgtcaccca ggtcacctcc cactcgcctt cccagagcag cgacggcttc
     2281 gcggtgtcgt ccggctggcc gtagaggatg atgcggggtt ttggcaaagt gccccagtcc
     2341 tgcgcgtcga gcgcacgctg cacggcgatc atgccgtaga gcggggcctc gaaggcgccg
     2401 gcgagttccg ccatcaccat ttcggccgga cgggaattgc ggcggaagat ctgtggcgac
     2461 ggcggctcgt tctcgatgcg caccacatcc ccgagttcca cgacgccgcg cgcgccaggc
     2521 agcacgttgg ccgggatggg gaccgaaagc gcccgttcgt cgagtaaccg gccggacttc
     2581 tccagcgcca cccggatcgg gatcggctgg cgacctccgc cccggtgcga atacccgact
     2641 gtaacgcccc catagagcag acggatggta tcgaacacat cccgctgctc gaccttgtgg
     2701 aactccagtt gctcatccac gaccgtcaga cggcggcgct gcggttgcag cgcgagcgtg
     2761 tcgtccacat cgacaatgaa gggaacggac ttgaaggcgg agcggacctt ctcggcgacc
     2821 tgtcggcgtg tttcgccatc cggaccgtag atttctgcca gcagggtcgc aagcacgggc
     2881 ggaccgggcg gcggctccac caccttgacc accgtgcctt gcggcagtgt ggccgccttc
     2941 aggcgttcac gcacctccag ggcaacatcg tggctgggcc gcgagcgctt atcccgggaa
     3001 accaggttca gttgcaggtc gcccatccag gtctcggacc gcaggtaggt atggcgcacc
     3061 agtccattga aattgaacgg cgccgccgtt ccggcatggg cctgcaccgt gtccacgtcc
     3121 ttcacgccgc gcagcgcatg ggcagcgtcc tgcaaggcgc gatccgtatc ctccacgctc
     3181 gcgccgcgcg gcaggtcgag catgaccgcc agttccggct tgtcgtcgaa ggggagcagc
     3241 ttgaccgtga ccgactgggt gtagaacagc gccagcgagc cgagggtgag cacgccggta
     3301 aggatgagga aagtccaggc ccggggcttg gtcgccagca cggggcgcgc cgacttgcga
     3361 tacatccgtc cgagccagcc ctcgtcgtga tgtccgcctt gcccgccgcc atgcgcgcca
     3421 ccgccaagtt tcaccatgag ccacggcgtc accatcaccg cgacaaagaa ggaaaaaacc
     3481 atagcggcgg atgcgttcga agggatcggg ctcatgtagg gtcccatgag gcccgacacg
     3541 aacagcatcg gcagcagggc cgcgaccacg gtcagcgtgg cgacgatnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnccc agatggaaca gaagttcgtt ggccttgtgg
     3721 ttcgcggtct cgccgtagtt gcgcgtcaca tgcacggtca cgtccgccgg cacgatcccg
     3781 cccctggcgc gctcgaccgc ggtgatgatc tgctgcgcga tcgtcacggc attggcccct
     3841 gcccgcttgg cgatggcgat ggagaccgcc ggggtgcgga cgaaggcgcc ctgcgcgtcg
     3901 cgctgcaggt gccaaaccat gcgttcggcc gggtcgcccg ccagccgcac gctggcgacg
     3961 tcacgcagat agaccggcct gtcgtcgcgc gtcgtgacgg aaatgttggc gatgtcctcg
     4021 atctcggcga gcgtgcggcc ggcgatcagc gtgatctgct ggccgccttc ccggatcgaa
     4081 ccggcgggaa aggcgcggtt ggcgctttcc accttggcca ccagagcttg caacgtcagc
     4141 ccgtagcgcg ccagtttctc cggatccggc acgattcgga tctcgtcgct ctgctcgccc
     4201 acgagatagg tcagccctac atcctggagc ttggcgatct cgatgcgcag ttcgcgcgcc
     4261 aggcgcgtca acgccgtcgc gctccagcgc tccgctgcct caggctttgg cgccagcgtg
     4321 agcacgacga tggccacgtc gtcgataccg cgaccgacga ccaggggctc gggaataccg
     4381 gccggaatcc ggtcaagatt ggcgcgcagc ttttcatgga ccctgagaat ggccgcatcg
     4441 gacgaggtgc cgaccacgaa gcgcgcggtg acaacggcac cgtgatcgga cgtctgcgaa
     4501 tagacgtgct cgacgctgtt gatggatttg acgatggctt ccagcggctc agtcaccagc
     4561 ttcaccgcgt cttccgcctt cagaccgtcg gcgcggacat ggatgtccac catcggcacg
     4621 gaaatctgcg gttcctcctc gcgcggcagg gcgacgaggg cgaccatccc cagcgccagc
     4681 gccacgatca ggaacagcgg cgtcagcggc gatgcgatga aggcgcgcgt cagcgcgccg
     4741 gcaatgccca gcggcggtct ggtcacggca caatcacccg atcgccggcc ttcaggcccg
     4801 acagcacctc gacacccggc ccctgcggcg tcgcgacagg gccgcccgcc acgatgacga
     4861 tgtcgcgccg gccgttcggg gtcgccaagg cgacgaaatc gacgccggcg cgatgcgcga
     4921 tcgctgacga cggcaccgcc agcgctttgc gcgtcgccac ggggacatgc acgagaacac
     4981 gctcgccgac aaaaaagtcg ccgagagcct cgacctcgac atcggcgatc accttgccgt
     5041 tctcgagttg cggaaaaatc ttcacaagcc ggccgctgga cgccgcagcg ccgggacgtc
     5101 cgtccacagg cacggttgca ttgaccttca gggtggccgc gaaccgctcc ggcagcgcga
     5161 ggcggaggaa aaacccgccg cccgcgatcg tcgccacggg ttcacccggc aggatcacag
     5221 agccacgtgt tgcggcccgg ctgagcaccc ggcccgtggc gggagcaacc accgccccct
     5281 cggccgactg ctgcatgatg accgccttgt ccgcttccgc cgccgcgatc tggttgcgga
     5341 acacctcata ggccgttcgc gtttgatcga cacgctgctg ggtgccggcg cctcgcgaaa
     5401 gcaggtcgcg cgcccggtcg tactcggtcc gggcattggc ctgctccgta gtcagcgccc
     5461 ggatgcgcgc ttccgccgcg ttgagctgca acgccagctt gtcgtcggca acgagggcga
     5521 tccgctcgcc ctgcttcacc gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nncgcgcgcc gagcttcttg aagatcatcg ccgccggaca gaacccggtg aaggccgact
     5701 gcagcatgtt caggccgatg aaaacggtga acaaccaaaa ccagggcgaa accagttgcg
     5761 tcagcacaac cgagagcagg atcatgacgc cggcgaaggc catcacggct ttgtcgatac
     5821 acatatgaga atctcctata gaagcaccgt ctgctcgcta tatataagaa atttctaatt
     5881 tatcaagtgc cggattcccg caaggctctt acggtgctat ctgcatggcg gtcggaaggg
     5941 cctgtccatg caagtggtct gcacgcgcca tgccgtatcg aacggcgatg atgtggaggc
     6001 gccccacagg cgctcgtttg actttccgga cggcacggag ctgccggacg tcctcacggc
     6061 gatcgcccgg tcggggtggt tgccgggaat tgccggcggc cgggcaacct ggtcggtcgc
     6121 gtcaggccgg ctcgtcgccg tcatcgcgca ggagtggcaa gcacccaagt tcctgccgat
     6181 cattgaggct ccatcgctgg actggcgcga cggcgccctg tttctccact tcaactatca
     6241 cgcgcaaatc gatccggaga cggtctaccg gatattctgg ggcatgcggt tgcaggcctg
     6301 agggcacgcc actgcggatc cggcgcacga gtcggtcggc ccaggattgg ggcggaggtc
     6361 tggctggcta ggcctgcgcg gcataggctg cgagccggct ggccaccaca tcaagcgctg
     6421 catcgcgcgc agaatcgcgc gtggacctga gataggcagc ggaaagcggc aggcggctgc
     6481 gcgtggcctg agaggcatca gcgagaggcc ggaagaccac tccgggtacg gcaaggcgcg
     6541 ccgcccagcg cggtacgatg gccaggccaa tgccagccgc gacaagattg acgatggtct
     6601 gcttttcatc ggcgacctgc gccacgcggg cgccgaaccc ggcatcggca aacagtttca
     6661 cagtcagatc atggctgtgg ggccgtgcgc ggcgatccgg cacgatcatc ggctggcctc
     6721 gcaggtcctc gaccgtgatg gcatcgcccc gcgccagcgc atgcccttca tggagtgcga
     6781 caaccgccgt ctcgaagaac aggtggcgga agacgaggcg cggatcgcgg gtttccggtg
     6841 gacgcaagaa ggcgaggtcc agccggccgg agagcaggcg cggcaacagt cggatggtct
     6901 tgtcctcgac catgcggacc tcgagccccg gatgtgcggc gcgcagatcc tgcagcaaca
     6961 gaggcaggag gcctgcagcg gcgctgtcga tcgcgccgat gcgcagcagc attgcgcggt
     7021 cacgggcctg
//
LOCUS       DAOMXN010000140         6987 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_5663_length_6987_cov_11.055684, whole genome shotgun sequence.
ACCESSION   DAOMXN010000140 DAOMXN010000000
VERSION     DAOMXN010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 6987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6987
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5663_length_6987_cov_11.055684"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..345)
                     /locus_tag="PK812_09870"
     CDS             complement(4..345)
                     /locus_tag="PK812_09870"
                     /inference="COORDINATES: protein motif:HMM:NF027439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRJ69906.1"
                     /translation="METMTVHSPSPLTRRDDSGVCVLTLARPSTRNALSEAMLDALAA
                     AIHAIREDDDIRAVVLAAEGPVFCAGHDLKEMTARRADPDRGRAYFADIMARCAAMMT
                     AIPAVKPRITG"
     gene            385..879
                     /locus_tag="PK812_09875"
     CDS             385..879
                     /locus_tag="PK812_09875"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRJ69907.1"
                     /translation="MTASKSARPQAPAMSIDEFNAFMDEVFPAAGHQAGGRRMVVEAM
                     GYGSCTVRLVFSEKQVRPGGTISGPTMMGLSDFAIYVAVLSAVGKVPLALTTNLNINF
                     LKAPAPRDMIARCRLIKVGKRLCVGEAELTSDGDDEMVAHATATYSIPPDRGGRPQGL
                     SPPV"
     gene            1011..1700
                     /locus_tag="PK812_09880"
     CDS             1011..1700
                     /locus_tag="PK812_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HRJ69908.1"
                     /translation="MPAFGHIDTWVFDLDNTLYPPHADLWPKVDAKITAYLADMFGID
                     GLSARALQKHYYQRYGTTLNGLMNEHSDALDIHHFLDFVHDIDRSSIAVDDALAAAIE
                     RLPGRRLVFTNGSRGHAEKTMIALGIAHLFDDVFDIVAGSLIPKPKAETYERFLAAHE
                     VEPKRAVMFEDIARNLEVPKLLGMATVLVVPKAGGGDHRDEVDVVRCAPTYVDHVTDD
                     LAAFLGRVLAR"
     gene            1768..2625
                     /gene="dapD"
                     /locus_tag="PK812_09885"
     CDS             1768..2625
                     /gene="dapD"
                     /locus_tag="PK812_09885"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HRJ69909.1"
                     /translation="MDTASLERIIEKAWDIRAEITPATKGEVRDAVMETLNLLDAGTL
                     RVATRAPGQSGPKAWTVHQWLKKAALLSFRLTDMGIIKGGPGNAVWWDKLPSKFEGWD
                     AARFQAGGFRTVPNCVVRHSAHIAKNVILMPSFVNLGAFVDEGTMVDTWATVGSCAQI
                     GKNVHLSGGVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVGEGSVLAMGVFISA
                     STKIIDRATGQVHVGYVPPYSVVVSGNMPGKPLPDGSPGPSLYCAVIVKTVDAQTRSK
                     TGINELLRD"
     gene            2629..3786
                     /gene="dapE"
                     /locus_tag="PK812_09890"
     CDS             2629..3786
                     /gene="dapE"
                     /locus_tag="PK812_09890"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HRJ69910.1"
                     /translation="MGIDPVALTQALVRCPSVTPAEGGALALLADVLRQAGFEVHRPV
                     FSEPGTPDIENLFARIGSGAPCLVFAGHTDVVPPGDGQKWSRDPFSGDIADGVLHGRG
                     ACDMKAGVAASISATLRHLAAVGLARGSIVFLITGDEEGPAVNGTVKLLKWAKARGEV
                     FSACVLGEPTNPDALGDMIKIGRRGSLTGHVIVRGRQGHVGYPHLALNPVDSMVPLLA
                     ALKARPLDAGTEFFDASNLEVTTVDTGNPATNVIPAECRATFNIRFNDLWSPQTLAEE
                     IRSRLAAVGAPATYDLRFDPTNSVAFLTRPGRFVDLVQAAVEAETGRTPALSTTGGTS
                     DARFIKDYCDVVEFGVVGKTMHQIDESVAVADIEALTRIYERIIDAFFAQD"
     gene            3828..4979
                     /locus_tag="PK812_09895"
     CDS             3828..4979
                     /locus_tag="PK812_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009756282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRJ69911.1"
                     /translation="MFTFNTTKSIVCGAGAMGRLAEVVGPLLGERILVVSDKGLIRLG
                     FLEKPLAALRAAGVAVAVFDEVEADPPEANILAGVAAAKAHGATGVLAIGGGSPMDVA
                     KVIALIAGSGESLSEIYGIGMAKGPRLPLALVPTTAGTGSEVTPIAIITTGEAEKKGV
                     VSPILLPDLAILDAELTLGLPAAVTAATGIDAMVHAIEAYASKSANNNALSRGLARQA
                     LQLLGAHIHTAVFEGTNTKAREAMLIGSMLAGQAFANSPVAAVHALAYPIGGHFHVPH
                     GLSNALVLPHVLRFNLPAARAAYSEIAADAFPELAALPMASRGERFVEALADLSRELQ
                     VPQRLRDVRIPESALPLLARDAMKQTRLLVNNPRAMNEAEALAIYAAAF"
     gene            complement(4983..6215)
                     /gene="rmuC"
                     /locus_tag="PK812_09900"
     CDS             complement(4983..6215)
                     /gene="rmuC"
                     /locus_tag="PK812_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014299795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HRJ69912.1"
                     /translation="MLLIVAGHTVTLRDAVIAGGVLALVLLALLLWLSARGASARAAE
                     AAAATERAREMDDKIAQLNQLQAEMTGRIQTFTEVLGSRQSDLARVVTDRLDSVKSSL
                     GQGLMQTAEKQAENLQKLHERLAVIDAAQANLVGLTSEVLVLKDILANKQARGAFGQG
                     RMEAIVRDGLPSSAFSFQATLANKTRPDCLIHLPGETRPMVIDAKFPLESFALLKEAG
                     SEDQRIAAERRVRADVGLHIKDIAEKYLVPGETQDLALLFVPSEALYADLCERFEDVV
                     QRAHKARVIIVSPSLLMMAIQVMQAIVRDARMRDQARVIQTEVARLLDDVRRLQERAG
                     KLEQHFRQAQEDVAGLLVSSEKVSKRGEKIEALEFDETPDAAPASAPQAQAPAAPRPA
                     VIGDVLPSPLFRRSGRGA"
     gene            6329..6853
                     /gene="def"
                     /locus_tag="PK812_09905"
     CDS             6329..6853
                     /gene="def"
                     /locus_tag="PK812_09905"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013164899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRJ69913.1"
                     /translation="MSIRKLVILPDPLLRKVSDPVRAVDKEMRALVDDMFETMYDAPG
                     IGLAAVQVGKPIRLFTLDVGRKTGVEKNPMVFINPEIVWRSEDVSTHEEGCLSIPDYY
                     TDVERPARIRVKYQDMDGKAHELEADGILSTCIQHEFDHLNGVLFIDHISKLRKMMVM
                     KKFTKAARLREAGI"
     gene            6895..>6987
                     /locus_tag="PK812_09910"
     CDS             6895..>6987
                     /locus_tag="PK812_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRJ69914.1"
                     /translation="MTLRIVFMGTPDFAVPVLAEIIGQGHEVVAV"
BASE COUNT         1183 a         2292 c         2264 g         1248 t
ORIGIN      
        1 gccctatccg gtgatccttg gcttcacggc ggggatcgcg gtcatcatcg cggcgcagcg
       61 cgccatgata tcggcgaaat aggcccggcc gcgatcggga tcggcgcggc gcgcggtcat
      121 ttccttcaag tcgtgcccag cgcagaagac cggcccttcg gcggccagaa ccacggccct
      181 gatatcgtcg tcctcgcgaa tggcgtggat cgccgcggca agcgcgtcga gcatcgcttc
      241 cgacagggcg ttgcgggtgg acgggcgggc gagcgtcagc acgcagacgc cggaatcgtc
      301 ccggcgcgtc agcggcgaag gggagtggac ggtcatggtt tccatgttac ctgttcgcgt
      361 cttcgacaat ctgagcatgg aatcatgacc gcgtccaaat ccgctcgccc gcaagcgccg
      421 gcgatgtcca tcgacgagtt caatgccttc atggacgagg tgtttcctgc agccggccat
      481 caggcgggcg ggcggcgcat ggtcgttgag gcgatgggct acggctcgtg cacggtccgt
      541 ctcgtcttca gcgagaagca ggtgcgcccc ggcggcacga tttccggccc gaccatgatg
      601 ggcctgtcgg acttcgccat ctatgtcgcc gtgctcagcg ccgtgggcaa ggtgccgctg
      661 gcgctgacca ccaacctcaa catcaacttc ctcaaggccc cggctccccg ggacatgatc
      721 gcacgttgcc ggctcatcaa ggtcggcaag cggctctgcg tgggcgaagc cgaactcact
      781 tccgacggcg acgacgagat ggtggcgcac gccacggcaa cctattcgat tccgccggac
      841 cggggcggca ggccgcaagg gctttcccca ccggtttagg cgacggcgcc aacaaaaccg
      901 caacatttgc cgcatagact gccgggatgt ttctcgacag aaccgacacg atgtccgaat
      961 gtgcaggcga tgatgcggcc cggcgactgg gccgcgaagg aatctccggc gtgcccgcct
     1021 tcggccatat cgacacttgg gtctttgacc tcgacaacac gctctacccc ccgcatgcgg
     1081 acctctggcc gaaggtggac gccaagatca cggcctatct cgccgacatg ttcggcattg
     1141 acgggctgtc cgcgcgcgct ttgcagaagc actattacca gcgctacgga accaccctga
     1201 acggtttgat gaacgagcat tcggatgcgc tcgacatcca ccatttcctg gatttcgtgc
     1261 acgacattga ccgttcgagc attgctgtgg acgatgctct ggcagccgcc atcgagcgcc
     1321 tgccggggcg tcgtctcgtg ttcaccaacg gctcgcgcgg ccatgccgag aagaccatga
     1381 tcgcgctcgg catcgcccac ctgttcgacg atgtgttcga catcgtcgcc ggcagcctga
     1441 tccccaagcc caaggccgag acctacgagc ggttcctcgc ggcgcatgag gtcgagccga
     1501 aacgcgcggt gatgtttgag gacatcgccc gcaatctgga agtgccgaag ttgcttggca
     1561 tggccacggt actggtcgtg cccaaggccg gaggcgggga ccatcgcgac gaggtggatg
     1621 tggtccgatg cgccccgacc tatgtcgacc acgtcacgga cgaccttgcc gcgtttctgg
     1681 gccgcgtctt ggcccgctga cccttgacgg cgcgcccgtg ccggtcgatg aacgcgccga
     1741 ccatatcctt taggaccgga cggcgccatg gacaccgcat cgctcgaacg catcatcgaa
     1801 aaggcctggg acatccgggc cgagatcacg cctgccacca agggcgaggt ccgcgatgcg
     1861 gtgatggaaa cgctcaacct cttggatgcg ggcacgctgc gcgtcgccac ccgggccccg
     1921 ggccagtccg gccccaaggc ctggaccgtg caccagtggc tgaagaaagc ggcactgctc
     1981 tccttccgcc tcaccgacat gggcatcatc aagggtggcc ccggcaacgc cgtgtggtgg
     2041 gacaagctgc catcgaagtt cgagggctgg gacgcagcgc gctttcaggc cggcggcttt
     2101 cgcaccgtgc cgaactgtgt ggtgcgtcat tccgcgcata tcgcgaagaa tgtcatcctg
     2161 atgccgtcct tcgtgaatct cggcgcgttc gtggacgagg gcaccatggt cgacacctgg
     2221 gcgacggtcg gctcctgcgc gcagatcgga aagaacgtgc acttgtcggg cggcgtcggc
     2281 atcggcggcg tcctggagcc gctgcaggcg aaccccacca tcatcgagga caactgcttc
     2341 atcggcgcgc gttcggaagt ggtggaaggc gtgatcgtgg gcgaaggatc ggtgctggcc
     2401 atgggcgtgt tcatttccgc ctcgaccaag atcatcgatc gcgccaccgg ccaggtgcat
     2461 gtcggctacg tgccgccgta ttcggtcgtc gtctccggca acatgcccgg taagccgctg
     2521 ccggacggct cgcccgggcc ttcgctctat tgcgccgtta tcgtcaagac tgtggatgcg
     2581 cagacgcgtt ccaagaccgg catcaacgaa ctcttgcgcg actgatccgt gggcatcgat
     2641 ccggttgccc tgacacaggc gcttgtccgc tgtccctcgg tgacgcccgc cgaaggcggc
     2701 gccctcgcgc tgttggcgga cgtgctgcgg caggccggct tcgaggtgca caggccggtg
     2761 ttttccgaac ctggcacgcc ggacatcgag aacctctttg cccggatcgg ctcgggcgcg
     2821 ccgtgcctcg tcttcgccgg ccataccgac gtggttccgc ccggggatgg tcaaaagtgg
     2881 tcgcgtgatc cgttttccgg cgatatcgcg gacggtgtgc tgcacggacg cggcgcctgc
     2941 gacatgaagg cgggcgtcgc ggcgagcatt tcggccacgc tgaggcacct tgccgccgtg
     3001 gggcttgccc gcggctccat cgtgttcctg atcaccggcg acgaggaagg gccggcggtc
     3061 aacggcacgg tgaaacttct gaaatgggcc aaagcgcggg gcgaggtctt ctccgcctgc
     3121 gttctgggcg agcctacgaa ccccgatgcc ctcggagaca tgatcaagat cggccggcgc
     3181 ggttccctca ccgggcatgt catcgtccgg ggccggcagg gacatgtcgg ctatccgcac
     3241 cttgccctga acccggtcga ctccatggtt ccgctgctgg ccgccctgaa agcccggccg
     3301 ctggacgccg ggaccgagtt cttcgacgct tcgaacctcg aagtcaccac ggtggacaca
     3361 ggcaatccgg cgaccaatgt cattccggca gaatgccggg ccaccttcaa catccggttc
     3421 aacgacctgt ggtcgccgca gaccttggcg gaagagatcc gaagccggct ggcggcggtg
     3481 ggcgcgccgg cgacctatga cctgcgcttc gatccgacga attcggtggc gtttctgacc
     3541 agaccgggcc gtttcgtcga tctcgtgcag gcagcggtcg aggccgagac cgggcgcaca
     3601 ccggcgcttt ccacaaccgg cggcacatcc gatgcgcggt tcatcaagga ctactgcgat
     3661 gtcgtcgagt ttggcgtggt cggcaagacc atgcaccaga tcgacgagag cgtggcggtg
     3721 gccgacatcg aggcgctgac ccgcatttac gagcggatca ttgacgcctt cttcgcgcag
     3781 gattgagata ggctgacctt tccgacgctc tgctggtgag ccgcatcatg ttcacgttca
     3841 acaccacgaa atccatcgtc tgcggcgcgg gcgccatggg gcgcctggcg gaggtcgtcg
     3901 gcccgctgct gggcgaacga atcctggtcg tgtccgacaa ggggttgatc aggcttggat
     3961 ttctggaaaa gcctctggcg gctctgaggg cggcgggcgt cgctgtcgcg gttttcgatg
     4021 aggtcgaggc cgatccaccc gaggccaaca tcctcgccgg cgtggcggcg gcgaaggccc
     4081 atggcgccac cggcgtgctc gcgatcggcg gcggttcgcc catggatgtg gcgaaagtca
     4141 ttgccttgat cgccggtagc ggcgaaagcc tgtccgagat ttatggaatc ggcatggcga
     4201 agggtccgcg cctgccgctg gcgctggttc cgaccactgc cggcaccggg tccgaagtca
     4261 cgccgattgc catcatcacc accggcgaag ctgagaaaaa gggcgttgtc tcgccgattc
     4321 ttttgcccga tctggccatc ctcgatgcag aactgaccct cggcctgcca gccgccgtga
     4381 cggccgccac cggcatcgac gccatggtcc acgccatcga ggcctatgcc tcgaaatccg
     4441 cgaacaacaa cgccctttcg agaggtttgg cacgacaggc tttgcaactg ctcggcgcgc
     4501 atatccatac ggccgtgttc gaaggcacga acacgaaggc gcgtgaggcg atgctcatcg
     4561 gctcgatgct ggcggggcag gctttcgcca attcccccgt cgccgcggtg catgctctcg
     4621 cctatccgat cggggggcat ttccatgtgc cccatggctt gtcgaacgcg ctggtgttgc
     4681 cgcacgtgct gcgcttcaac ctgccggccg ccagagcagc ctacagcgag atcgccgctg
     4741 acgccttccc ggaactcgcc gccctgccga tggcgtcgcg cggcgagcgt ttcgtcgagg
     4801 ccctggccga cctgtcgcgc gaattgcagg tcccgcagcg cctgcgcgat gtcaggattc
     4861 cggaatcggc gctgccgctg ctggcgcgcg acgccatgaa gcagacacgt ctcctggtga
     4921 acaatccacg cgcgatgaac gaggccgaag cgcttgcaat ctatgccgcc gcgttctgag
     4981 gatcacgcac cgcgcccgct ccgccggaac aggggcgaag gcaggacatc gccgatcacg
     5041 gcaggcctcg gcgctgccgg ggcctgagcc tgcggcgcgc tggctggcgc cgcgtccggt
     5101 gtctcgtcga attccagcgc ctcgatcttc tcgccgcgct tcgagacttt ctctgacgac
     5161 acaagcaagc cggcgacatc ttcctgcgcc tgacggaagt gctgttccag cttgccggca
     5221 cgctcctgca agcgtcgcac atcgtcgagc agccgggcga cctcggtctg gatgacgcgc
     5281 gcctggtcgc gcatgcgggc atcgcggacg atggcctgca tcacctggat cgccatcatc
     5341 aagagcgagg gcgagacgat gatcacccgc gccttgtggg cgcgctgcac cacatcctcg
     5401 aaccgttcgc acaggtccgc atagagcgct tcggaaggca cgaacagcag cgccaggtcc
     5461 tgtgtctcgc cggggacgag gtacttctcg gcgatgtcct tgatgtgcag gccgacatcg
     5521 gcgcgcaccc gtcgttccgc tgcgatgcgc tggtcctcgc ttcccgcctc cttcaggagg
     5581 gcgaacgatt cgagcggaaa ctttgcgtcg atcaccatgg gccgcgtctc gcccggcagg
     5641 tggatcaagc aatccggtcg cgtcttgttg gccagcgtcg cctggaacga gaacgccgag
     5701 gagggcagcc cgtcgcggac gatcgcctcc atccggccct ggccgaaggc gccgcgcgcc
     5761 tgcttgttgg cgaggatgtc cttcagcacg agcacttcgc tggtcaggcc gacgagattg
     5821 gcctgcgccg cgtcgatcac cgccaggcgc tcgtgcagct tttgcaggtt ttccgcctgc
     5881 ttttcggcgg tctgcatcag gccctgtcca aggctggact tcaccgaatc gagccggtcc
     5941 gtcacgacgc gggccaggtc gctctggcgc gaaccgagca cctcggtgaa ggtctggatg
     6001 cgaccggtca tctccgcctg caactggttg agctgggcga tcttgtcatc catctcgcgg
     6061 gcgcgctcgg tcgcggcagc cgcttctgcc gcgcgggcgg aggcgccacg cgcgctcagc
     6121 cacagcaaca gcgccagcag caccagtgca agcacgccgc cggcaatgac cgcatcgcgc
     6181 aacgtcacgg tgtgcccggc aacaatgagc agcatctcgt tcatcgttcc tgcgtatcag
     6241 agattcgctg ccatgtgaac gaaaacggaa cgcattgacc ggcggaaagc tgaagtctat
     6301 ggggaaaacc gcccggagct cgcctgccat gtccattcga aaactcgtca ttctgcctga
     6361 cccgctgctg cgcaaagtgt ccgatccggt ccgtgccgtc gacaaggaga tgcgcgcgct
     6421 cgtcgacgat atgttcgaga cgatgtacga tgcccctggc atcggacttg cggccgtgca
     6481 agttggcaag ccgatccggc ttttcaccct cgacgtcggc cgcaagacgg gcgttgaaaa
     6541 gaacccgatg gtgttcatca acccggagat cgtctggcgt tccgaagacg taagcaccca
     6601 cgaggagggg tgcctgtcga tccccgacta ttacaccgac gtggagcggc ccgcgcgcat
     6661 ccgggtgaag taccaggaca tggacggcaa ggcccatgag ctcgaggcgg atggcatcct
     6721 ctcgacgtgc atccagcatg aattcgacca tctgaacggc gttctgttca tcgaccacat
     6781 ctccaagctc aggaagatga tggtgatgaa gaagttcacc aaggccgccc ggctgcgaga
     6841 ggcgggcatc tgagcgccgg tccgtcgggg cggcgcgatg acgggacctg aaccatgacc
     6901 ctgcgtatcg ttttcatggg cacgccggat ttcgccgtgc ccgtcctggc cgagatcatc
     6961 gggcaggggc acgaggttgt ggcggtc
//
LOCUS       DAOMXN010000141         6910 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_5748_length_6910_cov_9.010212, whole genome shotgun sequence.
ACCESSION   DAOMXN010000141 DAOMXN010000000
VERSION     DAOMXN010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 6910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6910
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5748_length_6910_cov_9.010212"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(277..468)
                     /locus_tag="PK812_09915"
     CDS             complement(277..468)
                     /locus_tag="PK812_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018264293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69915.1"
                     /translation="MTSVTDSIIARGDLAHLVLFLWASGASALLALSLRESFAASRRF
                     DDFVRELARFNSRNGAGNE"
     gene            569..1195
                     /locus_tag="PK812_09920"
     CDS             569..1195
                     /locus_tag="PK812_09920"
                     /inference="COORDINATES: protein motif:HMM:NF012273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRJ69916.1"
                     /translation="MAELEIVGAPMSNYVWSVRVACEEKGVPYKLAVVRPHSPEALAC
                     GPTGKIPGLRHGEVALSESKALISYIDRAFKGRKLMPAKPAAMAEVEQWCSIVNTAVD
                     PAWRRYIACYFFPQTPDKKPDPAAIAAAVPGLEKVLGMLEARLTASRHLAAGKFSPAD
                     MLLVPMLVYLQKLPESSKLLKKHKKLNSYLKRLAKRPSVAATRPKEAN"
     gene            1254..1475
                     /locus_tag="PK812_09925"
     CDS             1254..1475
                     /locus_tag="PK812_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69917.1"
                     /translation="MSAPVRPLPAVGTPGRHARLVGVELADESGESLLGFRRQGVQVD
                     HLATRGDQHVPIGALAVADGLALLVRQTN"
     gene            complement(1479..1811)
                     /locus_tag="PK812_09930"
     CDS             complement(1479..1811)
                     /locus_tag="PK812_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69918.1"
                     /translation="MTEARKTTLEFTISPAALTRIVQLRDESAEKLGETGLIPSVAWG
                     FVVSDGKKEHEGFVIGLYPGSMRGEISRILHQVDGVEVAIFLMDEKLAEMHGKTLHFR
                     EESGFTLI"
     gene            complement(2228..2581)
                     /locus_tag="PK812_09935"
     CDS             complement(2228..2581)
                     /locus_tag="PK812_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69919.1"
                     /translation="MKNSGVGMSVNFSTKAEEYVRAVWNMMLDAGVSPESNILIITFP
                     LATWNAREPDKRTERAPNGGPLVSIGWISKDKLNGEHRVMAGDTTIAIRRDSRTQSAH
                     SLAFDYNGQLLLSMS"
     gene            complement(2970..4115)
                     /locus_tag="PK812_09940"
                     /pseudo
     CDS             complement(2970..4115)
                     /locus_tag="PK812_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018045747.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            4261..6237
                     /locus_tag="PK812_09945"
     CDS             4261..6237
                     /locus_tag="PK812_09945"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRJ69920.1"
                     /translation="MGQHADGKDDLLEDVREAEHGAGSLLREQYISLTRLVPVLYTVV
                     IIATVAISIAFTGTGPAWLVNWIPAAMLVVVVLRLRYWLKARAKATTQDVAIMKRDIR
                     GTTILGPALTLGFTLIGIGLMPYADTYQQSLAVVAIWITAVASAFCLAALPNASLMIV
                     VSAGVPLSISFLWSGNDLMMMLAGVFAVVTGLVVYMLRENYAAFREIVMSRFEIDEKH
                     RSAEEARKAATVMAYTDFLTNLSNRRHFEVLLNDRVSSHSLAEKPFAVGILDLDGFKP
                     INDVHGHGVGDSVLFEVASRLALAMNGRGRAARMGGDEFAVLAEGIASEDEALALAAH
                     IRDALSQPYQIGPGLTAEMTCSMGFALYPSSSINPARLIDHADMALYQCKARQRGSAA
                     VFSAEHEDAAVERAQIEQGLRRAIAEKALTVYFQPIVDLSSGRLTGFEALARWRDPVL
                     GYVSPAVFIPIAERAGLIEALTDDLLYKAARAAASWPDNLVLSFNLSADQLVKESAGL
                     RIVKILADAGLPPHRFEAEVTETAIMRDIAAARRTLESLKSAGARVALDDFGTGYSSL
                     SQIKDLPLDKVKIDKSFVDQIVNDPKMRNLVRAIISMCDNLELRCVAEGIEQEDQLTA
                     LQSGGCASGQGYLFSRPIPIDEVRALIETKRRAA"
     gene            complement(6238..>6910)
                     /locus_tag="PK812_09950"
     CDS             complement(6238..>6910)
                     /locus_tag="PK812_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018329565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRJ69921.1"
                     /translation="NAYNLAPDFLSRIVGRYAGTRLGRQELDGEFIEDRADTLFPRAT
                     IEASRLDKAPTCRRIVVAVDPPAGGKRAGGCGIIVAGIAADGLVHVLADCSLAGARPD
                     VWAAAALSAYHAHEADALIAEVNQGGAMVGEVMRSIDASVPVQPVRATRGKAVRAEPV
                     AALYAQGRVKHAGTFPALEDEMALFGPDGLSGGKSPDRLDALVWAVTALTAETAGGTP
                     RVRRM"
BASE COUNT         1169 a         2319 c         2220 g         1202 t
ORIGIN      
        1 gatcacctga agggccaggg cgcgaaggac ctggctccgg ctgcggtcgc tcgaagcgct
       61 ccagcaggtc gaagaagcgg cggaacacgc gctgcggttc cgggcgcgcg gcgggctgtc
      121 ccggccgacc ggagaggcgc ggcaggtcgg tgaacttccg gtaggtcatg gcagaatccg
      181 gggttagagg gcgaccaacg aaaacggcgg cccgaaggcc gccgctgaag gcgaagacag
      241 tgcgtaattc cgcgccgttg tgcagcatcg tcatgctcat tcgttcccag ccccgttgcg
      301 gctgttgaag cgggcgagct cgcgcacgaa atcgtcgaag cgtcggctgg cggcgaagct
      361 ttcgcgcaag gagagcgcca gcagcgcgct cgccccgctg gcccagagga acagcacgag
      421 atgggcgaga tcgccgcgcg cgatgatgct gtcggtgacg gaggtcatcg gcgtggctcc
      481 tggctcagtg tttggacggc caggcccggt ccccttgcca agcggaacgc gctcggccac
      541 cttgcgcgcc tcgaagaggg agggattcat ggcggaactg gaaatcgtcg gcgcgccgat
      601 gtcgaattat gtctggtcgg tgcgcgtcgc ctgcgaagaa aagggcgtgc cctacaagct
      661 ggcggtggtg cggccgcatt caccggaggc gctggcctgc ggcccgacgg gcaagatccc
      721 cggcctcagg catggcgagg ttgcgctcag cgagtcgaag gcgctgatca gctacatcga
      781 tcgcgccttc aagggcagga agctcatgcc ggccaagccg gctgcgatgg ccgaggtcga
      841 gcaatggtgc tccatcgtca acacggcggt cgatccggcc tggcggcgct acatcgcctg
      901 ctatttcttc ccgcagacgc cggacaagaa gccggaccct gccgccatcg ccgccgccgt
      961 gccggggctc gaaaaggttc tcggcatgct ggaggcgcgg ctcaccgcga gccgccacct
     1021 cgccgccggc aagttctcac cggccgacat gctgctggtg ccgatgctgg tctatctgca
     1081 gaagctgccg gaatcgtcca agctgctgaa gaagcacaag aagctgaaca gctatctcaa
     1141 gcggctcgcg aagcgtccgt ccgtcgccgc gacccggccg aaagaggcga actgagcaac
     1201 gatcgagggc tgacattgcc gtcagccgct cggcttcgtc ctggccgggc aaaatgtcgg
     1261 cccccgtccg tccactgccc gccgtcggca ctcccggccg gcacgcgcgg ctggtcgggg
     1321 tcgaacttgc ggatgagtcc ggcgagagcc tccttggctt ccgccggcag ggcgtccagg
     1381 tcgatcacct ggccacgcga ggagaccagc acgtccccat cggcgcgctt gccgtagccg
     1441 atggcctcgc gcttctcgtt cgacagacga attgacgttc agatgagcgt aaatccgctc
     1501 tcctcccgaa aatgcagcgt ttttccgtgc atttctgcca gtttttcgtc cattaggaag
     1561 atggcaacct cgactccgtc tacctgatgc aggatgcgtg aaatttcgcc ccgcatgctc
     1621 cccgggtata ggccgatgac aaaaccttcg tgttcctttt ttccgtcgct gaccacgaat
     1681 ccccaagcga cagacggaat taggcccgtc tcaccaagct tctcagcaga ctcgtcgcgc
     1741 aattgaacaa ttcgggtgag ggctgccgga gagatggtga attccagcgt cgttttgcga
     1801 gcctcagtca tggcgttcac ctgcgactat cgttgagaag ggttccgtgc caagttcccg
     1861 cgtaggtcgt ccagggcatg caggccgcgt agtttgggcc aggaataata acatggcggc
     1921 caccgatgtc gtatacgcag tatctgccgc ccccagtata gggaagctcg gcaacaagcg
     1981 tagcgatccc ggctgaagcg atacgcacaa tctggtcgcc gaccgaaccg ctccctccgc
     2041 ccccgccccc gtccgtccac tgcccgccgt cggaactccc ggcaggcacg cgcggctggt
     2101 tgggatcaaa cttgcgcagg agggaggcga gagcctcctt gctcgccggc gtcagcccat
     2161 cgaggtcgat cacctggctg cgggacgaga ccagcacgtc cccgtccggc cgcttgccgg
     2221 gatacgatca actcatcgag agcagcagtt gcccattgta gtcaaaggca agggagtgcg
     2281 ctgattgcgt tcgtgagtca cggcgaatag caatggttgt gtcaccggcc ataaccctgt
     2341 gttcaccgtt cagcttgtct ttcgatatcc agccaatcga gaccaatggg ccgccattcg
     2401 gcgctcgctc ggtgcgcttg tcaggctcgc gggcgttcca ggttgccaag gggaatgtga
     2461 taatcagaat attcgactcc ggcgaaacgc ctgcatccag catcatgttc caaaccgcgc
     2521 ggacatattc ctctgccttg gtcgaaaaat ttacgctcat tccgacgcca gagtttttca
     2581 tgggagaccc ctgttcttcg tttcaagcat cgtcaaagca acggtccgat atttgcgtcc
     2641 ggacaagtaa catgcccttc atatgccaag ccgacaacct tgtcatatga gcagagtgtc
     2701 cacgcagaag gccccttctc aaaccactcc tgcttaatca gcctgccaat tccaatgttg
     2761 gcggccaccg tcgttgtagg accgcctccc cctcccccgc ccctgtccgt ccactgcccg
     2821 ccgtcggcgc tgccggcggg cacgcgtggc tggttgggat caaacttgcg cagcagcgtg
     2881 acgagcaccg ccttggcctc cggcggcagc ccatcgaggt cgatcacctg gccgcgggac
     2941 gagaccagca cgtccccgtc cggccgcttg ccgtagccga tggcctcgcg cttctcgttc
     3001 accgtgaggt aatcggcgcg cagcatcgcc ttcatcttcg cctcgcgctc ctcggccagc
     3061 gcctcgatgt ggtcgaggtc gaggtccagc ctgagaccgc cgccgaagcc cggggagagc
     3121 cagtgcgcca gcgcttccgt gactcttcgg gcgagcggga tcacggtttg gcgccagagc
     3181 gcgcggtttg cctcggcgta gttggcgtgc gtgttgtcgc cgggaaggcc gagcagcagc
     3241 ggcggcacgc cgaaggcgag cgcgatctcg cgcgcggcgg cagacttcgc cgccatgaag
     3301 tccatgtcgc ggggcgagag cgccagcggc ttccagtcga gcccgccttc gagcagcatc
     3361 ggccgcccgg cgttgagcgg tccggagaaa gccccttcca gttcctcctt cagccttgcg
     3421 aactgctcat ccgtcatgtt ggccccgccc tgggcgtaga ccagcgcgcc ggaaggacgg
     3481 gcggaattgt ccaggagcgc cttgttccag gcgctggcgg aattgtggat gtcgagcggc
     3541 acctgcgcag cgctcagcgg ggcgaggccg tagtggtcat cgagcgggtc ggcgaatttg
     3601 agatgcagga cgggcggaat gccctcgcca tcgcagcgca gggcaatggt ctggccgcca
     3661 gccgtgtatt cgtaggcctc gggccagccg tccgggccgg gcacgatctt cacccggtcg
     3721 gggcgcaagc tgtaaagttc gcgcggcacg ccggcctccg cgaccatctc gacataggca
     3781 ttgccggtga gcagcatgtt ggtgaacacc tgctccatca ggtcgcggcc gccctggcgc
     3841 ggattgggcc gggacagcaa cagttcgagc gggtgattgc gcaactcgtc gcggtcccgg
     3901 aagacatagg ccgggatcgc agcggccgct tcggccacca gccgcacgca gcggaacacc
     3961 acggcgttct tggcatagcc ctcgcgcatc aggtccgacg tgtcgcggct ggaccagatc
     4021 ggccggccga caccggccaa ggcgatcagc ggcgccgcgc gggagacctt cgcttcccgg
     4081 gccggacgtc ggagcatctt caggaaatcg ggcatgatgt acgccttaag gttgctttga
     4141 gtgatttatg cgatccctgc gtgtgctttg tgacagatcg ctcaaatccg aacgatctcg
     4201 ctcgcagttg cgaaacacgc cgctggtatg agcttcacgc acagaggggc aaggacaggc
     4261 atgggccagc acgcggacgg caaggacgat cttctcgaag acgtccggga agcggaacac
     4321 ggcgccggtt ccctgctgcg cgagcagtac atttcgctca cgcggctggt gccggtgctg
     4381 tacacggtcg tcatcatcgc cactgttgcg atctccatcg ccttcaccgg aacgggaccg
     4441 gcctggctcg tgaactggat tccggcggcg atgctcgtgg ttgtcgtcct gcgcttgcgc
     4501 tactggctga aggcccgggc caaggcgacg actcaggacg tcgccatcat gaagcgcgac
     4561 atccgcggca cgacgatcct gggtccggcc ctgacacttg gattcacgct catcggcatc
     4621 ggcctgatgc cctatgccga cacctaccag cagtctctgg cggtggtggc catctggatc
     4681 accgccgtcg cgagcgcctt ctgtctcgcc gcattgccga acgcctcgct gatgattgtc
     4741 gtcagcgccg gcgtgccgct ctcgatttcg ttcctgtgga gcggcaacga cctgatgatg
     4801 atgctggccg gcgttttcgc cgtggtgacc ggtctggtcg tctacatgct gcgcgagaac
     4861 tacgcggcgt tccgcgagat cgtcatgtcg cgtttcgaga tcgacgagaa gcaccgttcc
     4921 gccgaggaag cgcgcaaggc cgctacggtg atggcctata cggactttct gaccaacctg
     4981 tcgaaccgcc gccatttcga agtgctgctg aacgatcgcg tcagcagcca cagccttgcc
     5041 gagaagcctt tcgccgttgg cattctcgat ctggacggct tcaagccgat caacgacgtg
     5101 cacggccatg gcgtcggcga ttccgtcctg ttcgaagtgg cttcgcgcct cgcgctggcg
     5161 atgaacgggc gcgggcgcgc ggcgcgcatg ggcggcgacg aattcgccgt tctggcggag
     5221 ggcatagcga gcgaggacga ggccctggcc ctcgccgcgc acatccgcga cgccctctcg
     5281 cagccctacc agatcggccc cggcctgacc gccgagatga cctgctccat gggctttgcg
     5341 ctctatccca gttccagcat caaccctgcc cgcctgatcg accatgccga catggcgctc
     5401 taccagtgca aggcgcgcca gcgcggctcg gccgcggtct tctcggccga gcatgaggat
     5461 gcggcggtgg aacgcgcgca gatcgagcaa ggcctgcgcc gggccatcgc cgagaaggcg
     5521 ctgaccgttt atttccagcc gatcgtcgac ctgtcgagcg gccggctcac cggattcgag
     5581 gcgctggcgc gctggcgcga tcccgtgctt ggctacgtct cgcctgcggt gttcattccg
     5641 atcgccgaac gggcgggcct gatcgaggcc ctcaccgatg atctcctgta caaggccgcc
     5701 cgcgctgccg cgagctggcc ggacaacctg gtcctgtcct tcaacctgtc cgccgatcag
     5761 ctggtgaagg agagcgcggg cctcaggatc gtgaagatcc tcgccgacgc cggcctgccc
     5821 ccgcaccggt tcgaggcgga agtcaccgag accgcgatca tgcgcgacat tgccgccgcg
     5881 cgtcgcacgc tggaaagcct gaagtccgcc ggcgcgcgcg tcgcgctcga cgacttcggc
     5941 accggctatt cctcgctcag ccagatcaag gacttaccgc tggacaaggt gaagatcgac
     6001 aagagcttcg tcgatcagat cgtcaacgat cccaagatgc gcaacctggt gcgcgccatc
     6061 atctcgatgt gcgacaatct cgaactgcgc tgcgtcgccg aaggcatcga gcaggaagac
     6121 cagctcaccg ccctgcaatc cggcggctgc gcctccggcc agggctacct gttctcgcgg
     6181 ccgatcccga ttgacgaggt ccgcgcgttg atcgagacca agcgccgcgc ggcgtgatca
     6241 catcctccgc acccttggcg tcccgcccgc cgtctccgcc gtcagcgcgg tgacggccca
     6301 gaccagcgcg tcgagccggt ccggcgactt gccgccggaa agcccgtccg ggccaaacag
     6361 ggccatctcg tcctccagcg ccgggaacgt gccggcatgt ttcacccggc cctgcgcgta
     6421 gagcgcggcc accggctcgg cgcgcaccgc cttgccgcgc gtcgcccgca ccggctgcac
     6481 cggaacggat gcatcgatgg aacgcatcac ctctcccacc atggcgccgc cctggttgac
     6541 ctcggcgatc agcgcgtccg cctcgtgggc atggtaggcg gaaagcgccg ccgcggccca
     6601 gacatccggc cgcgcgccgg cgagcgagca atccgccagc acgtggacca gcccatccgc
     6661 cgcgatgccg gcgacgatga tgccgcagcc cccggccctt tttcccccgg ccggcggatc
     6721 gaccgccacg acgatgcgcc ggcaggtcgg cgccttgtcc agccgagacg cctcgatcgt
     6781 ggcgcgggga aacagcgtgt ccgcgcggtc ctcgatgaat tcgccgtcga gttcctgccg
     6841 gccgagccgc gtgccggcgt agcgcccgac gatgcggctg aggaaatccg gcgcgaggtt
     6901 gtaggcgttc
//
LOCUS       DAOMXN010000142         6883 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_5776_length_6883_cov_10.622876, whole genome shotgun sequence.
ACCESSION   DAOMXN010000142 DAOMXN010000000
VERSION     DAOMXN010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 6883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6883
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5776_length_6883_cov_10.622876"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="PK812_09955"
     CDS             <1..528
                     /locus_tag="PK812_09955"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF023780.2"
                     /note="catalyzes the DNA-template-directed extension of
                     the 3'-end of a DNA strand; the tau chain serves as a
                     scaffold to help in the dimerizaton of the alpha,epsilon
                     and theta core complex; the gamma chain seems to interact
                     with the delta and delta' subunits to transfer the beta
                     subunit on the DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HRJ69922.1"
                     /translation="PQLAVSNPQPLQPPRASAQPHLAEVAPAMRIRRFEDIPAIAAEK
                     RDIALKVAIERDLRLVSFEEGRIEINVVSGAAADLSTRLTRALQAWTGQRWIVALSQD
                     EGQPTLHEQEQARKASDLRGVRAHPTVRAVLEAFPGAEIVRVDLKRPESEAVDGAGDA
                     AQAVPIDPDDSDDDL"
     gene            544..864
                     /locus_tag="PK812_09960"
     CDS             544..864
                     /locus_tag="PK812_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003614007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HRJ69923.1"
                     /translation="MRDMMGMMKQVQAMQAKFSAMQAEMDQIEVEGQAGGGMVKVVLT
                     AKGALKGVRIDPSLLREGEGEILEDLIVAAHADAKSKTEKVMEEKMKALTGGLPLPPG
                     MKLF"
     gene            961..1566
                     /gene="recR"
                     /locus_tag="PK812_09965"
     CDS             961..1566
                     /gene="recR"
                     /locus_tag="PK812_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004684611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HRJ69924.1"
                     /translation="MSQSIAGPEIERLIQLLARVPGLGPRSARRAALHLIRRKDELLA
                     PLASAMQVAAERIVTCCVCGNIDTADPCTICRDTRRDPTILVVVETVADLWALERAKA
                     LNARYHVLGGVLSPLDGVGPKDLNIEPLLTRVAEGGVREVIVALNATVDGQTTAHYLT
                     DLLSGLDVTVTRLAHGVPVGGELDYLDEGTLAAALRSRTAM"
     gene            complement(1573..1902)
                     /locus_tag="PK812_09970"
     CDS             complement(1573..1902)
                     /locus_tag="PK812_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69925.1"
                     /translation="MMRIALMLVACAAILPAPAEAQQRRARSASEVIVVNARQATLTE
                     FQLTQDDGSVVGAIKAPLPPGKRVTLKLTKGAPCLLTISALYDDEFENAGAQIDACKD
                     KVVRFTN"
     gene            complement(2022..3176)
                     /locus_tag="PK812_09975"
     CDS             complement(2022..3176)
                     /locus_tag="PK812_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRJ69926.1"
                     /translation="MSITSNWNYPTPVKFGPGRIRELPALCRANGIDRPLLVTDPGLA
                     RLPMIDAAMASLKAAGLGAGLFSAIKPNPNGQNVEDGVTAYRTGGHNGVIAFGGGSAL
                     DAGKAIAFMSGQTRPLFDFEDVGDNFLRADVKGIAPSIAVPTTAGTGSEVGRVSVITD
                     EATHLKKLIFHPKILPVCVIADPELTLGVPPAITAATGMDALAHCLEAYCAPGFHPMA
                     DGIALEGMRLIRDFLPIAVKDGKNIEARARMMAAATMGAAAFQKGLGAIHSLAHPLGA
                     IYDAHHGLLNAILMPYVLEFNRPAIGERMERLARYLALPKAGTDGVIDWVLDLRREIG
                     TPHTLSAIGIGEDRADQIAKAALGDPSTPGNPVELTEANLKTLFVKSVRG"
     gene            complement(3200..4588)
                     /locus_tag="PK812_09980"
     CDS             complement(3200..4588)
                     /locus_tag="PK812_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRJ69927.1"
                     /translation="MTGLKVVSPVDGRIYVERTLASEAEIAAALQRAEAAQRLWRVVP
                     VAERAKAMSAFVDAFVSKREAIAEELTWQMGRPLRYTPGEVRGFEERARMMISLAPEA
                     LADVQVSEKAGFTRFIRRTAVGVVLVVAPWNYPLLTAVNAVVPALMAGNAVLLKHAAQ
                     TPLCSERFAEAAKAAGLPEGLLQALHMSHDATARLIRAPAIQHVCFTGSVEGGAAMER
                     AASGRFIGVGLELGGKDPAYVRADANLDHAIENLVDGAFFNSGQSCCGIERIYVHAKL
                     YDKFVEGAAALTRTYVLGDPTKAETTLGPLVRASAADFVRGQTEEALRQGATALIDEK
                     AFPASKAGTPYLAPQVLIGVDHAMRVMSEESFGPVVGIMKVTSDEEAIALMNDSAYGL
                     TAAIWTSDAEAAASIGDRIETGTVFMNRCDYLDPELAWTGVKNTGRGVTLSRLGYDHL
                     TRVKSFHLRTQI"
     gene            complement(4590..5966)
                     /locus_tag="PK812_09985"
     CDS             complement(4590..5966)
                     /locus_tag="PK812_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HRJ69928.1"
                     /translation="MADPVPFRFKNAADAAAYVREKNLPNVKVGVTDMDGILRGKYLS
                     RDKFLSALESGFGFCDVIVGWDSNDQLYDNVTYTGWHTAYPDAPVRILPETGRALPFE
                     NNIPFFLCELSDKAEAICPRGVLRKVLAKADSMGLKASAAIEYEFFVFDETPDSVRAK
                     GYRDLKPITPGYFGYSVLRSSVHGELYHDLLDLCRVMDMELEGLHTETGPGVLEAAIV
                     VDDAIRAADKANLFKNFVKVFAQRRNLMATFMAKWSGNWPGQSGHIHISLKDKKGKAV
                     FHDGKQKHAISKTMRHFIGGQQALMPKILAMVSSTVNSYSRLVPGYWAPTSATWSIDN
                     RTTALRAIPGSEKSQRVEYRIAAADANPYLALAAALGSGLWGIENEIEPAPAVEGNAY
                     AQKFPAKLGLPGTLWEAAQRLKAAKEARALFGEAFVEHFAATREWEEREFRRHVTDWE
                     LARYFEII"
     gene            complement(5978..6766)
                     /gene="hisN"
                     /locus_tag="PK812_09990"
     CDS             complement(5978..6766)
                     /gene="hisN"
                     /locus_tag="PK812_09990"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HRJ69929.1"
                     /translation="MPGVDAARLRAFEDFALRLADAAKPVALRYFRKPLAIDTKADES
                     PVTIADREIETALRTLIQATHPDHGILGEEHGRSGLDREFLWVIDPIDGTKSFISGMP
                     TFGVLIALLQHGRPLLGIADHPAMGERFVGIAGAQTRWNGAGCRVRDCRRLKDAILFA
                     TTPDMFEGDAAAPFERVSRAAKVRRFGGDCFAYAMLAGGHVDAVVEAQLQPYDYMALV
                     PMVEGAGGIITDWTGAPLTIDSDGRVIAAATASLHKEIRDQLNP"
BASE COUNT         1091 a         2289 c         2329 g         1174 t
ORIGIN      
        1 ccgcagctgg ccgtctccaa cccccagccg ctccagccgc cgcgcgcctc ggcccagccg
       61 catctggcgg aagtggcgcc ggcgatgcgc attcgccgct tcgaggatat tccggccatt
      121 gccgccgaga agcgcgacat tgcgctcaaa gtcgcgatcg aacgcgatct gcgcctggtg
      181 tccttcgagg aagggcgcat tgagatcaat gtggtttccg gcgcggcggc cgatctgtcg
      241 acgcggctga cgcgcgccct gcaggcctgg accggccagc gctggatcgt cgccctttcg
      301 caggacgagg gacagcccac gctccacgag caggaacagg cccgcaaggc gtccgatctg
      361 cgcggcgtta gggcgcatcc gacggttcgc gccgtgctgg aagcctttcc gggcgcggag
      421 atcgtgcgcg tcgatctgaa gcggccggag agcgaggcgg ttgacggcgc cggcgatgcg
      481 gcccaagcgg taccgatcga tcccgacgat tccgatgatg atctctaagg ggacagtttg
      541 gccatgcgcg acatgatggg catgatgaag caggttcagg ccatgcaggc gaagttttcc
      601 gccatgcagg ccgagatgga tcagatcgag gtcgaggggc aggccggcgg cggcatggtc
      661 aaggtcgtcc tcaccgccaa gggcgcgctg aagggcgtgc ggatcgatcc gtccctgctg
      721 cgcgagggcg agggtgagat cctggaggat ctgatcgtcg ccgcccatgc cgacgccaag
      781 tccaagaccg agaaggtcat ggaagagaag atgaaggcgc tcaccggcgg gctgccgctg
      841 cctcccggca tgaagctgtt ctgaggccgg ttgcgggaat cgccgccggg tgacacagct
      901 ttccgctccc cgcccgggga taaggaagat ctgccgcagc gcctgtaacg caccgtcacc
      961 atgtcccagt ccatcgccgg cccggaaatc gagcgcctga tccagttgct ggcacgcgtg
     1021 ccgggcctgg gtccgcgctc ggcccggcgc gccgcgctgc atctgatccg ccgcaaggac
     1081 gagttgctgg ccccgctcgc ttccgccatg caggtcgcgg cggagcgcat cgtcacctgc
     1141 tgcgtctgcg gcaacatcga taccgccgat ccctgcacca tctgccgcga cacacgccgc
     1201 gatcccacga tcctggtcgt ggtcgaaacc gtggccgatc tctgggcgct ggagcgcgcg
     1261 aaggcgctga atgcgcgcta tcacgtgctc ggaggcgtct tgtcgcctct ggacggcgtc
     1321 ggcccgaagg atctcaacat cgagccgctg ttgacccgcg tcgctgaagg gggcgtgcgc
     1381 gaggtcatcg tcgcgctgaa cgccacggtc gacgggcaga ccacggcgca ctacctcacc
     1441 gatctcctgt ccggactcga tgtcacggtc acgcgcctcg cgcatggtgt gccggtcggc
     1501 ggtgaactcg actatctcga tgagggcacg ctcgccgccg cgctgagaag ccggaccgcg
     1561 atgtagcggc gctcagttcg tgaagcgcac caccttgtcc ttgcaggcgt cgatctgcgc
     1621 gcccgcgttc tcgaactcgt cgtcatagag cgcggagatc gtcagcaggc agggcgcgcc
     1681 cttcgtcagc ttcagcgtga cccgcttgcc cgggggcagc ggcgccttga tcgccccgac
     1741 cacactcccg tcatcctggg tcagctgaaa ttcggtcaga gtcgcctggc gcgcattgac
     1801 gacgatgacc tcgctggcgc tgcgggcgcg ccgctgctgc gcctcagccg gcgccggcag
     1861 aatggcggcg caggccacga gcatgagtgc gatacgcatc atcggacacc ccgtttcgag
     1921 tcgcaccttg cggctcgcgc ggatgctacg cccggttcat caaacgggca accagccgtg
     1981 gacggcaggg ccgcgtccgt cgatgtggtc ctggccgtgc gttatccccg cacggacttc
     2041 acgaacaacg tcttcaggtt ggcttcggtc agttccaccg ggttgcccgg cgtcgagggg
     2101 tcgccgagcg ccgccttggc gatctggtcc gccctgtcct cgccgatgcc gatggcggag
     2161 agcgtgtgcg gcgtgccgat ctcccggcgc aggtccagca cccagtcgat cacgccgtcg
     2221 gtgcccgcct tcggcagggc gagatagcgg gcgagccgct ccatgcgctc gccgatcgcc
     2281 gggcggttga actccagcac gtagggcatg agaatggcgt tcagcagacc gtgatgcgca
     2341 tcgtagatcg cgccgagcgg atgggcgagg gagtggatgg cgccgaggcc cttctggaag
     2401 gcggccgcgc ccatcgtggc ggcggccatc atccgcgcgc gcgcctcgat gttcttccca
     2461 tccttcaccg cgatcgggag gaagtcccgg atcagccgca tgccttcgag cgcgatgccg
     2521 tcggccatcg ggtggaagcc cggcgcgcaa taggcttcga ggcagtgcgc cagcgcgtcc
     2581 atgccggtgg cggcggtgat cgccggcggc acgccgagcg tcagttcggg atcggcgata
     2641 acgcagaccg gcaggatttt cgggtgaaag atcagcttct tcaggtgcgt cgcttcatcc
     2701 gtgatcaccg agacgcgccc gacttccgac cccgtgccgg cggtggtcgg aaccgcgatg
     2761 gacggggcga tgcccttcac gtccgcacgc aggaaattgt cgcccacgtc ctcgaaatcg
     2821 aacagcggcc gggtctggcc ggacatgaag gcaatggcct tgcccgcgtc gagcgcgctg
     2881 ccgccgccga aggcgatgac gccattgtgc ccgcccgtgc gataggccgt cacgccgtcc
     2941 tcgacattct ggccattcgg gttcggcttg atggcggaga acagacccgc gccaaggccg
     3001 gccgccttca ggctcgccat cgccgcatcg atcatcggca gcctggcaag gcccggatcg
     3061 gtgacgagca gcggccggtc gatgccgttg gcccggcaca gggcgggcag ttccctgatc
     3121 cggccagggc cgaatttcac cggggtgggg tagttccagt tggatgtgat cgacattggg
     3181 gcatccggta gggcgagtgt cagatctgcg tgcgcaggtg aaacgatttc acgcgggtga
     3241 ggtggtcgta tccgagcctt gagagcgtca cgccccggcc ggtgttctta acgccggtcc
     3301 aggcgagttc cgggtcgagg tagtcgcagc ggttcatgaa cacggtgccg gtctcgatgc
     3361 ggtcgccgat ggaggccgcg gcttccgcgt cggaagtcca gatcgcggcg gtgaggccat
     3421 acgcgctgtc gttcatcagc gcgatcgcct cttcgtccga tgtcaccttc atgattccga
     3481 cgaccgggcc gaagctttcc tcgctcatca cgcgcatcgc gtgatcgacg ccgatcagca
     3541 cctgcggcgc gagatagggc gtgcccgcct tcgaggcagg gaaggccttc tcgtcgatca
     3601 gcgcggtcgc gccctggcgc aaggcttcct ccgtctggcc gcgcacgaaa tcggcggcgc
     3661 tcgcgcggac caggggccca agcgtcgtct cggctttcgt cggatcgccg agcacatagg
     3721 tacgcgtcag cgccgccgcg ccttccacga acttgtcgta gagcttggcg tgcacataga
     3781 tgcgctcgat gccgcagcag gattggccgg agttgaagaa cgcgccgtcg acgaggttct
     3841 cgatggcatg gtcgagattg gcatccgcgc gcacataggc cgggtccttg ccgccgagtt
     3901 cgaggccgac gccgatgaac cgcccgctgg ccgcgcgttc catcgccgcg ccgccctcga
     3961 ccgaaccggt gaagcacacg tgctggatcg ccggcgcgcg gatgagccgg gccgtcgcgt
     4021 cgtggctcat gtgcagcgcc tgcagcaacc cctcgggcag gccggcggcc ttcgcggcct
     4081 cggcgaagcg ctcggaacag agcggcgtct gcgccgcgtg cttcagcaac accgcattgc
     4141 ccgccatcag cgccggtacc acggcgttta cggcggtcag cagcggatag ttccacggag
     4201 cgaccaccag caccacgccg acggcggtgc ggcggatgaa gcgtgtgaag ccggccttct
     4261 cggacacctg gacgtccgcc agggcttccg gcgcgagcga gatcatcatc cgcgcccgct
     4321 cctcgaagcc gcgcacttcg cccggcgtgt agcgcagcgg acggcccatc tgccaggtca
     4381 gctcctcggc gatggcctca cgcttcgaga cgaaagcgtc gacgaaggcg ctcatcgcct
     4441 tcgcccgctc cgccactggc acaacacgcc aaagacgttg cgccgcctcg gcgcgctgga
     4501 gcgcggcggc gatctccgcc tcgctcgcca gtgtgcgttc cacatagatg cgcccgtcga
     4561 cgggcgaaac gactttcagg ccggtcatgt cagatgatct cgaaatagcg cgccagttcc
     4621 cagtcggtga catggcggcg gaactcgcgt tcttcccatt cgcgcgtcgc ggcgaagtgc
     4681 tcgacgaatg cctcgccgaa cagcgcgcgc gcctccttcg cggccttcag tcgctgcgcc
     4741 gcctcccaga gcgtgcccgg cagaccgagc ttggccggga acttctgggc ataggcgttg
     4801 ccttcgaccg ctggcgcggg ttcgatctcg ttctcgatgc cccacagacc ggagccgagc
     4861 gcggccgcga gcgccagata cgggttcgcg tcggccgccg cgatgcgata ctcgacgcgc
     4921 tgcgattttt cgctgccggg aatggcgcgc agcgcggtgg tgcggttgtc gatgctccag
     4981 gtggctgaag tcggcgccca gtagcccgga accagccgcg aataggaatt caccgtcgag
     5041 gataccatcg ccaggatctt gggcatcagc gcctgctggc cgccgatgaa atggcgcatg
     5101 gttttcgaga tggcgtgctt ctgcttgccg tcgtggaaaa cggccttgcc cttcttgtcc
     5161 ttgagcgaga tatggatatg gccgctctgg cccggccagt tgccggacca tttcgccatg
     5221 aacgtcgcca tcaggttgcg gcgctgggcg aagaccttga cgaaattctt gaacaggttg
     5281 gccttgtcgg cggcgcggat ggcgtcgtcc acgacgatcg cggcctccag gacccccgga
     5341 ccggtttcgg tgtgcaggcc ctccagctcc atgtccatga cgcggcagag gtcgagcagg
     5401 tcatggtaga gctcgccatg caccgaggat cgcagcacgg aatagccgaa atagcccggc
     5461 gtgatcggct tcaggtcgcg atagccctta gcgcgcacgc tgtccggcgt ctcgtcgaac
     5521 acgaagaact cgtactcgat ggcagcggaa gccttcagcc ccatgctgtc ggccttggcc
     5581 agcaccttgc gcagcacgcc gcgcgggcag atcgcctccg ccttgtcgga caattcgcac
     5641 aggaagaacg ggatgttgtt ctcgaacggc agtgcccggc cggtttcggg caggatgcgc
     5701 accggcgcgt cgggataggc cgtgtgccag cccgtatagg ttacgttgtc gtagagctgg
     5761 tcgttggagt cccagccgac gatcacgtcg cagaagccga agccgctctc cagcgccgag
     5821 aggaacttgt cgcgcgacag gtatttgccg cgcaggatgc cgtccatgtc ggtgacgccg
     5881 accttgacgt tgggcagatt tttctcccgc acataggcgg cggcgtcggc ggcgttcttg
     5941 aagcggaagg gaaccgggtc ggccattgct caccctctta tggattgagt tgatcgcgga
     6001 tttctttgtg caggctcgcg gtcgccgcgg caatgacccg cccgtcggag tcgatggtca
     6061 gtggcgcgcc cgtccagtcc gtgatgatgc cgccggcacc ctccaccatg ggcaccagcg
     6121 ccatgtagtc atagggctgg agctgcgctt ccaccacggc gtccacatgc ccgccggcca
     6181 acatggcata ggcgaagcag tcgccgccga accggcgcac cttcgccgcc cgcgaaaccc
     6241 gctcgaacgg cgccgccgca tcgccctcga acatgtctgg cgtggtcgcg aacaggatcg
     6301 catccttcag cctgcggcag tcgcggaccc ggcagcccgc gccgttccag cgcgtctgcg
     6361 ccccggcgat gccgacgaac cgctctccca tggcaggatg gtcggcgatg ccgagcagcg
     6421 gccgtccgtg ctggagcagc gcgatcagca cgccgaaggt cggcatgccg gaaatgaagc
     6481 tcttggttcc gtcgatcggg tctatgaccc agaggaactc gcggtcgagg ccgctgcgac
     6541 cgtgctcttc gccgaggatg ccgtgatcgg gatgggtggc ctggatcagg gtgcgaagcg
     6601 cggtttcgat ctcccggtcg gcaatcgtca ccggcgattc gtcggccttg gtgtcgatgg
     6661 cgaggggctt gcggaagtag cgcagcgcaa cgggcttcgc ggcgtcggcg agacgcaggg
     6721 caaaatcctc gaaagcgcgt aagcgcgccg catccacacc aggcattccg gaagcacctc
     6781 cagcgatgcg tcctaggtag cctcagttgg cgttatttgg caattcgtgg cgtttcgtcg
     6841 caggcggttt tctgcgcggg ccgcatcacg ctccggcgcg tac
//
LOCUS       DAOMXN010000143         6751 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_5942_length_6751_cov_9.603047, whole genome shotgun sequence.
ACCESSION   DAOMXN010000143 DAOMXN010000000
VERSION     DAOMXN010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 6751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6751
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5942_length_6751_cov_9.603047"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..964
                     /locus_tag="PK812_09995"
     CDS             152..964
                     /locus_tag="PK812_09995"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRJ69930.1"
                     /translation="MSAPTRPVIVVTGASSGIGRDLALTAKGELVLVARSAEVLAALA
                     QEIAAAGGTAHVLALDLSDPGSTARLVDFLAASGLHCEVLVNNAGFGLIGRADGLDRD
                     GQINMLDLNARSLADLTLAVLPGMLHRGRGGVLNVASVASFLPGPNMAMYYATKAFVR
                     SFSNALWQECKGRGVTVTALCPGPVATAFFTRATGGAAHAKGPKKKIPLIFRLIPPTT
                     SRDVALAGWAALAAGKHTIIPGPANRVAAAMTRFVPQRLLLAAVARFQKARH"
     gene            complement(988..1845)
                     /locus_tag="PK812_10000"
     CDS             complement(988..1845)
                     /locus_tag="PK812_10000"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRJ69931.1"
                     /translation="MSQTPAPPTAAQLADPAWCEAQYNPRLTVSDPLAILARYADWAA
                     QSRAALPFRTLSYGDGAREVLDYVPVAKPRGLIAYIHGGYWRAQHKDDYSWVADTFHA
                     AGYSVAVITYPLCPDVTIRDIVGCCEKAIARLWQELSESERARVLVTGHSAGGYLTAA
                     MFAADWRAHGLPASPFVGGVSYSGVFELPPLLNTSMNALIRLTPDQAQDWSMGRFSPR
                     VAALLILAYGADEPGEFHRQSIEQAAHWSTICRPAVPAIGCNHFTIVESLRDPGSQMA
                     RLTLEVIAR"
     gene            1910..3307
                     /locus_tag="PK812_10005"
     CDS             1910..3307
                     /locus_tag="PK812_10005"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRJ69932.1"
                     /translation="MNQTRTHIIGIAGIGMSATGLLLKERGHVVTGSDEGIYPPASVI
                     LPAAGIAVMSPHAATNIPADVDQFVIGKHAKLTAENPEVAAAMATGKPILSYPEVLAQ
                     LTRGRRNLVVAGSYGKSTSTALIAWALAANGREPGYFIGALPKNLATHAALGRHEAFV
                     IEGDEYPSSNTDPSSKFMHYAPSALLLTAAMHDHVNVFPTQEDYLRPFRALLRKMPSG
                     GLVVACLDEPFAAGLAREGHPGRTVFYSLENPAADYHARTISYGETTTFTLVHRGTAL
                     GAFSTTQLGAHNLQNMVGAAAMLIEEKLLSPQEIGDAFARFAGVMRRMDKLTAKSPIP
                     AYEGFGSSREKALSAIDAIRLHFPRKRLVIVFEPHTFSWRSRDALHWYDTVFREAAEV
                     FVYRPPHHGATAHNQASHAEITARIAATGLPTHALSGDEGAIVAALKPDDVVLILSSG
                     DMDGMIARLVRRMEG"
     gene            complement(3323..4165)
                     /gene="kynA"
                     /locus_tag="PK812_10010"
     CDS             complement(3323..4165)
                     /gene="kynA"
                     /locus_tag="PK812_10010"
                     /EC_number="1.13.11.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase"
                     /protein_id="HRJ69933.1"
                     /translation="MADNKTAPESWHAAKLDFSKDMSYADYLKLDDVLSAQHPLSPDR
                     NELLFIIQHQTSELWMKLMLHELGHAIVSVREEKLPQAFKMLARVSRIMEQLVKAWDV
                     LATMTPPEYSAIRPWLGQSSGFQSYQYREIEFRLGNKNRAMLKPHEHAPNRLAEVTAA
                     LEAPSLYDETVRLLARRGFAIDPKRLERDATLPTEFDQSVENAWAEVYREPETHWDLY
                     ELAEELVDLEDAFRLWRFRHVTTVERVIGFKRGTGGTAGVGYLRKMLDVVLFPELWRV
                     RTAM"
     gene            4326..6641
                     /locus_tag="PK812_10015"
     CDS             4326..6641
                     /locus_tag="PK812_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012093350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003959 - NADPH dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="HRJ69934.1"
                     /translation="MRIVCIGGGPAGLYFGILMKLSDPSHQITVVERNRPYDTFGWGV
                     VFSDATMDNMRLWDRKSADAIQEAFNHWDDIETWFKGRKIRTTGHGFVGIGRKKLLNI
                     LQERCEELGVELAFEREVDSDLEFPDADLIIASDGIGSKIRARYQDVFQQDIVVRPNR
                     YIWLGTRRLFDAFTFDFRKTEHGWFQAHIYKFDAETSTFIVETTEDTFHKHGLDKADQ
                     AQSVAFCEKVFAEILEGAKLETNARHLRGSAWLNFNRLICGKWSHFNGKSHVVLMGDA
                     AHTAHFAIGSGTKLAFEDAIELTNQFRRIGQARTDIPKVLEAYEAIRSVDAARLQNAA
                     RNAMEWFEVVERRYADAMEPEQFMYSMLTRSQRISHENLRLRDKDWLEGYERWFAAKA
                     GVPARPDERPLPPMFTPFTVRGLTLKNRIVMSPMAMYSAVDGVPGDFHLAHFGARALG
                     GAALLFSEMTAPAPDGRITPGCLGLWNDTQSAAFKRLIALVHASGAKFGIQIGHSGRK
                     GSTRVAWEGIDEPLASGGWEIIAPSALPYLRHGQVPRAMTRADMDRVRDAHVQAVKRA
                     ADAGADMIELHAAHGYLLSSFLSPLTNKRTDEYGGNHENRARFPLEVFAAMRAAWPKD
                     RPMAVRLSCHDWFEGGNTPEDALIFARMFKSAGADMIDCSSGQVVKEEKPVYGRLFQT
                     PFSDMIRNEAGIPTIAVGAISEADHANSIIAAGRADLCALARPHLADPAWTLHEAAKI
                     GLTVIAWPNQYRSGKMQYETNLARAATPAKG"
     gene            6646..>6751
                     /locus_tag="PK812_10020"
     CDS             6646..>6751
                     /locus_tag="PK812_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010659196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HRJ69935.1"
                     /translation="MALADQHALVTGGGTGIGAAIAATLARAGARVTIA"
BASE COUNT         1137 a         2154 c         2316 g         1144 t
ORIGIN      
        1 agcctcgcag cctgcgggcg acggccagcc cgatgccgct ggccgcaccg gtgacggcgg
       61 cgatttcgcc tgtgaaatca atattcatga tcgcgagcgt gcgccccttg ctgttcgttg
      121 ctataggcga ggcgcaagga aaggacaatc aatgagcgct cccacccgtc ccgtgatcgt
      181 cgtcaccgga gcgtcttccg gcatcgggcg cgatctggcg ctgactgcga agggcgagct
      241 tgtgctcgtc gcccgatccg ctgaagtgct ggctgcgctg gcgcaggaga ttgccgcagc
      301 gggcggcacg gcgcatgtgc tggcgcttga tctctcggat cccggctcga cggcgcggct
      361 ggtcgatttc ctcgcggcca gcggcctgca ttgcgaggtg ctggtcaaca acgccgggtt
      421 cggactgatc ggccgggcgg acgggttgga ccgcgacggg cagatcaaca tgctcgatct
      481 caacgcgcgc tcgctggccg atctgacgct cgccgtgctg ccgggcatgc tgcaccgcgg
      541 tcgtggcggc gtcctgaacg tcgcctcggt cgcttcgttc ctgccgggtc cgaacatggc
      601 gatgtattac gcgaccaagg cgttcgtccg gtcgttctcc aacgccctgt ggcaggaatg
      661 caaggggcgc ggcgtgacgg tgacggcgct gtgtcccggt ccggtcgcga cggcgttctt
      721 cacgcgcgcc acgggcgggg cggcgcatgc caaagggcca aagaagaaaa tcccgctgat
      781 cttccggctg atcccgccga ccacctcgcg cgatgtggcg ctggccggct gggccgcgct
      841 ggcggcgggc aagcacacca tcatcccggg accggcgaac agggtggcgg ccgcgatgac
      901 gcgattcgtg ccccagcgcc tgctgttggc cgccgtcgct cggttccaga aggcgcggca
      961 ctgaagccgc gatccggcaa agtgcgatca gcgcgcgatc acttccaacg tcagccttgc
     1021 catctgcgat ccgggatcgc gcaggctttc gacaatggtg aaatggttgc agccgatggc
     1081 gggaaccgcc gggcggcaga tcgttgacca gtgcgcggcc tgctcgatgc tctggcggtg
     1141 gaattcgccg ggctcgtccg cgccgtaggc gaggatcagc agcgcggcga cgcgcggcga
     1201 gaagcgtccc atgctccagt cttgcgcctg atccggggtg aggcggatca gcgcgttcat
     1261 cgaggtgttg agcagcggcg gaagttcgaa cacgccggaa taggagacgc cgccgacgaa
     1321 cggtgaggcg ggaagcccat gggcgcgcca gtccgccgcg aacatcgcgg cggtgagata
     1381 gccgccggcg gaatggccgg tgacgaggac gcgggcgcgc tcgctctcgg aaagctcctg
     1441 ccacagccgg gcgatggctt tctcgcaaca gccgacgatg tcgcggatcg tgacgtcagg
     1501 gcagagcgga taggtgatga ccgccacgct gtagcctgcg gcgtggaagg tgtcggcgac
     1561 ccaggaatag tcgtccttgt gctgggcgcg ccagtagccg ccgtgaatgt aggcgatgag
     1621 gcccctgggt ttggcgacgg gcacatagtc cagcacctcc cgtgcgccgt ccccgtagga
     1681 cagggtgcgg aacggcaagg cggcgcgcga ttgcgcggcc cagtcggcgt agcgcgcgag
     1741 aatcgccagc gggtccgaga ccgtgagacg cggattgtac tgagcctcgc accaggcggg
     1801 gtctgcgagt tgggcggcgg tcggcggggc aggggtctgg gacatgccgc tgttctcgca
     1861 agccgcgccg ggcaaggcaa gccggtctgc actctgctat acacaccgca tgaatcagac
     1921 gcgcacgcac atcatcggca tcgccggcat cggcatgtcc gccaccggcc tgctcctcaa
     1981 ggaacgcggc cacgtcgtca ccggctcgga cgagggcatt tatccgcccg cgagcgtgat
     2041 cctgccggcg gccggcatcg cggtgatgag cccgcatgcc gcgacgaaca tcccggccga
     2101 tgtcgatcag ttcgtgatag gcaagcacgc caagttgacg gctgagaatc cggaagtcgc
     2161 cgccgcgatg gcgacgggaa agccgatcct gtcctatccg gaagtgctgg cacagttgac
     2221 ccgaggccgc cgcaatctcg tcgtcgccgg cagttatggc aagagcacga gcacggcgct
     2281 gatcgcctgg gcgctggcag caaacggcag ggaaccgggc tatttcatcg gcgcgctgcc
     2341 gaagaatctg gcgacccatg ccgccctcgg ccggcatgag gccttcgtga tcgagggcga
     2401 cgaataccct tcgtccaata ccgacccctc ctcgaagttc atgcattacg cgccttccgc
     2461 cctgctgctc acggcggcga tgcatgacca tgtcaacgtg ttcccgacgc aggaggatta
     2521 tctccggccg ttccgtgcgc tgctgcggaa gatgccttcg ggcgggctgg tcgtcgcctg
     2581 tctcgacgag ccattcgctg cgggtctggc gcgcgagggc catccgggcc gcaccgtgtt
     2641 ttatagtctc gagaatccgg cggccgacta ccacgcccgg accatcagct acggcgagac
     2701 cacgaccttc acgctggtgc atcggggcac ggcgctgggc gcgttctcga ccacgcagct
     2761 cggcgcgcac aacctgcaga acatggtggg cgcggcggcg atgctgatcg aggaaaagct
     2821 gctgtctccg caagagattg gcgacgcttt cgctcggttc gccggagtca tgcggcgcat
     2881 ggacaagctg acggcgaaaa gcccgatccc ggcctacgag gggtttggct cctcgcgcga
     2941 aaaggcgctt tcggcgattg atgcgatccg gctgcatttt ccgcgcaagc ggctggtcat
     3001 cgtcttcgag ccgcacacgt tctcctggcg cagccgggac gcgctccatt ggtacgatac
     3061 agtgtttcgc gaggcggcgg aggtgttcgt ctaccgcccg ccgcaccacg gcgcgaccgc
     3121 gcataaccag gcgagccatg ccgagataac ggcgcgcatc gccgcgaccg gcctgccgac
     3181 ccatgcgctt tccggcgacg agggcgctat cgtcgcggcg ctgaaacccg atgacgtcgt
     3241 gctgatcctc tcgtccggcg acatggacgg catgatcgcg cggctggtcc ggcgcatgga
     3301 aggataggcg ccccgaggcg cctcacatcg ccgtgcggac ccgccagagt tcggggaaca
     3361 gcaccacatc cagcatcttc cggaggtagc cgacgccggc cgtgccgccg gtgccgcgct
     3421 tgaagccgat gacgcgctcg acggtcgtga cgtgccggaa gcgccagagc cggaaggcgt
     3481 cttccaggtc aacgagttcc tcggccagtt cgtagaggtc ccagtgcgtt tccggttcgc
     3541 ggtagacctc ggcccaggcg ttctccacgc tctggtcgaa ttcggtgggc agcgtcgcgt
     3601 cgcgctccag tcgcttggga tcgatggcaa agccgcgccg cgccagcagc cgcaccgtct
     3661 cgtcatagag cgagggggct tcgagcgccg ccgtcacctc ggccaggcgg ttcggcgcgt
     3721 gttcgtgggg cttcagcatc gcccggttct tgttgccgag gcggaactcg atctcgcggt
     3781 actggtagga ctggaagccg gaggactggc cgagccacgg ccggatcgcc gagtactccg
     3841 ggggagtcat ggtggccaac acgtcccagg ccttgaccag ctgttccatg atgcgagaga
     3901 cgcgcgccag catcttgaac gcctgcggca gcttttcttc gcgcaccgag acgatggcgt
     3961 ggccgagttc gtgcaacatc agcttcatcc agagttcgga cgtctggtgc tggataatga
     4021 acagcagctc gttgcggtcc ggcgacaacg ggtgttgcgc ggagaggacg tcgtcgagct
     4081 tgaggtaatc cgcgtaggac atgtctttcg agaaatcgag cttggctgca tgccagctct
     4141 cgggcgctgt cttgttgtcc gccatcgttg cctcccatat cgtttgcgcc agcatgaggc
     4201 cggccgagcg gtgcgcaagt ccgcgtcgtc gggcaaagag tcgcgcccgg cggcttgaca
     4261 aaacacttca agcctcaata cttcaagctt aaaataaatc gcgaaccgag ggggctggag
     4321 gaaccatgcg cattgtctgc atcggcggcg ggccggctgg cctgtatttc ggcatcctga
     4381 tgaagttgtc ggacccctcg caccagatca cggtggtcga gcggaaccgg ccctatgaca
     4441 cgttcggctg gggcgtggtg ttctccgacg cgaccatgga caacatgcgt ctgtgggatc
     4501 gcaagtccgc cgatgccatc caggaggcgt tcaaccactg ggacgacatc gaaacctggt
     4561 tcaaggggcg caagatccgc accacgggcc atggcttcgt cggcatcggc cgcaagaagc
     4621 tcctgaacat cctgcaggag cgctgcgagg aactcggcgt cgagctcgct ttcgagcgcg
     4681 aggtggacag cgatcttgaa ttcccggatg ccgacctgat catcgcctcg gacggcatcg
     4741 gctcgaaaat ccgggcgcgc taccaggatg tgttccagca ggacatcgtg gtccgtccga
     4801 accgttacat ctggctcggc acgcgccggc tgttcgatgc gttcaccttc gatttccgca
     4861 agaccgagca cggctggttc caggcgcaca tctacaagtt cgatgccgaa acctcgacgt
     4921 tcatcgtcga gacgacggag gatacctttc acaagcacgg gctggacaag gccgaccagg
     4981 ctcagtccgt ggccttctgc gagaaggttt tcgcggaaat cctggaaggg gcgaagctgg
     5041 agaccaacgc gcgccacctg cgcggctctg cctggctgaa cttcaaccgc ctgatctgcg
     5101 gaaagtggag ccatttcaat gggaaatcgc acgttgttct catgggggac gcggcccaca
     5161 cagcgcattt cgccatcggg tccggcacca agctcgcttt cgaggacgcg atcgaactga
     5221 ccaaccagtt ccgcaggatc ggtcaggcgc gcaccgacat tcccaaggtg ctggaggcct
     5281 atgaggccat ccgcagcgtc gacgccgcac ggctccagaa cgcggcgcgc aatgccatgg
     5341 aatggttcga ggtggtcgag cggcgctatg ccgatgcgat ggagccggag cagttcatgt
     5401 attccatgct gacgcgctcg cagcgcatca gccatgagaa cctgcgcctg cgcgacaagg
     5461 attggctcga aggctacgag cgctggttcg cggcgaaagc cggcgtgccg gcaaggccgg
     5521 acgaacggcc gctgccgccg atgttcacgc ccttcacggt gcgtggtctg acgctgaaga
     5581 accggatcgt catgtcgccg atggcgatgt attcggcggt cgacggtgtg ccgggcgatt
     5641 tccatctggc gcatttcggc gcgcgggcgc tgggcggcgc ggcgctgctg ttctcagaga
     5701 tgaccgcgcc tgcgccggac gggcgcatca cgccggggtg tctcgggctg tggaacgata
     5761 cccagtctgc ggcgttcaag cggctcatcg ccctggtgca cgcctcgggg gcgaaattcg
     5821 gcattcagat cggccattcc ggtcgcaagg ggtcgacccg cgtcgcctgg gagggcatcg
     5881 acgagccgct ggcctccggc ggctgggaga tcatcgcgcc ctcggcgctg ccatacctca
     5941 ggcacggcca ggtgccgcgc gccatgaccc gggccgacat ggaccgggtg cgcgatgccc
     6001 atgtgcaggc ggtgaagcgc gcagccgatg ccggcgcgga catgatcgag ctgcatgcgg
     6061 cgcacggcta tctgctctcg tccttcctgt cgccgctgac gaacaagcgc acggacgagt
     6121 acggcggcaa ccacgagaac cgcgcgcgct tccctctgga agtgttcgcg gcgatgcgtg
     6181 cggcctggcc gaaggaccgg ccgatggcgg tgcgcctgtc gtgccacgac tggttcgagg
     6241 gcggcaacac gccggaggat gcgctgatct tcgcgcggat gttcaagagc gccggtgccg
     6301 acatgatcga ctgctcctcc gggcaggtgg tgaaggagga gaagccggtc tacggtcgcc
     6361 tgttccagac gccgttctcc gacatgatcc gcaacgaggc cgggattccg accattgccg
     6421 tcggggcgat ctccgaggcc gaccacgcca attcgatcat cgccgccggc cgcgccgacc
     6481 tgtgcgcgct cgcccgtccg catctggccg atccggcctg gacgctgcat gaagcggcga
     6541 aaattggcct tacggtgatc gcttggccga accagtatcg ttcgggcaag atgcagtacg
     6601 agacgaatct ggcgcgcgcg gcgacgccgg cgaaagggta gggcgatggc tctggccgat
     6661 cagcacgcgc tggtcaccgg cgggggaacc ggcatcggtg ccgcgatcgc cgcaacgttg
     6721 gccagggccg gcgcgcgggt gacgatcgct g
//
LOCUS       DAOMXN010000144         6667 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_6055_length_6667_cov_11.172414, whole genome shotgun sequence.
ACCESSION   DAOMXN010000144 DAOMXN010000000
VERSION     DAOMXN010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 6667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6667
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6055_length_6667_cov_11.172414"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1471
                     /locus_tag="PK812_10025"
     CDS             <1..1471
                     /locus_tag="PK812_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRJ69936.1"
                     /translation="AAPNGKRVACIGGGPASLTVARDLAVLGYAVDVFDEGAALGGMM
                     RSQIPKFRLPDAVIDEECAYITGLGQVTVHRTRVNSLKEMLTRGYDAVFVGTGAPRGK
                     GLDIPGQEAGASNIHTGIEWLASVSFGHTTSIGRRVIVLGGGNTAMDCCRTARRLGGA
                     DVKVIVRSGFEEMKASPWEKEDAIHEDIPILNFLTPKAFVIRDGKLAGVTFEKVKAVY
                     DEKGRRSLVPTGEPDVTHDCDDVLVAIGQDNAFPWIERDAGIEFDRWGLPKLDEKTFR
                     STLPNVFFGGDAAFGPKNIIWAVAHGHDAAVSIHKYCQGEDIADRPPPGVSIISQKMG
                     IHEWVYDNDISVKARTPMPHRAKAEVLADIRAEVELGFDAALALREAQRCLNCDVQTV
                     FSAALCIECDACIDICPMDSLTIAPNGEEGDLRARLTAPALNLAQDLYISADLKTGRI
                     MVKDEDVCLHCGLCAERCPTGAWDMQKYLIQMTHAGCKP"
     gene            1468..3324
                     /locus_tag="PK812_10030"
     CDS             1468..3324
                     /locus_tag="PK812_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HRJ69937.1"
                     /translation="MNVHLRQPVTATNDFVVKFANVNGSGSASANELFARSILRMGVP
                     VSPRNIFPSNIQGLPTWYEVRVSGKGWLGRRGGCDMMVAMNPQTWAQDVKEIEPGGYL
                     VYDSTKPMGPEAFRDDITVIGLPLTAISNATYSDVRQRQLFKNIIGLGALSLLLGIDP
                     KAIEQLFGEQYKGKEALLASNVKALHLGRDYVEKNLDPNICRLKVVPANAVGERIFLE
                     GNMAAALGCVYGGATVCAWYPITPSSSLAEAFQSYCKRYRHDPDTGEAKYAIVQAEDE
                     LASIGMVIGAAWNGARAFTATSGPGISLMTEFIGLAYFAEIPAVLINVQRGGPSTGMP
                     TRTQQADILSCAYASNGDTKHVLLFPEDPTECFEMAADALDLADRLQTPVFVMTDLDI
                     GMNYRLCRPLRWDDSRKLDRGKVMTADELQAGKAFGRYTDVDGDGIPFRTYPGTHPTK
                     GAFFTRGTSKNRKAGYSEEGDVYVDNMERLLHKFETAKSLCPQPEFRAAARPTRTGVI
                     YFGSTAPAMDEALALLADEGAHFDAVRIRAFPFASSIDRFIADHDKVFVVEQNRDAQL
                     RSMLINEIGVDPARLVAVTHYDGTPITARFIVGAINANLDLARIRLNPEMLS"
     gene            3321..4376
                     /locus_tag="PK812_10035"
     CDS             3321..4376
                     /locus_tag="PK812_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HRJ69938.1"
                     /translation="MTYIAKPKLHHPSLTRNEAGYTRRDYEGPVSTLCAGCGHDSISA
                     AIIHACYELSIEPHRVAKLSGIGCSSKTPTYFLGNSHGFNTVHGRMPSVLTGANLANR
                     DLIYLGVSGDGDSASIGLGQFAHVMRRGVNMLYVVMNNGVYGLTKGQFSATADRGSKS
                     KKGAVNADSAIDLASLAILMGATFVARSFSGDKEQLVPLIKAAISHPGAAILDVISPC
                     VAFNNHAGSTKSYDYIREHNEAVNRLDFITGRAPITADYAPGEVREIMQHDGSMLRLR
                     KLDATYDPSSKAGALAHIAERQAAGEVATGLLYLDPSPRDLHAAQNTVKRPLNTLTEK
                     DLCPGSAALEKINAGLR"
     gene            4535..4681
                     /locus_tag="PK812_10040"
     CDS             4535..4681
                     /locus_tag="PK812_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69939.1"
                     /translation="MPSARFFFFSLLVVSIMGLVLPTDALASSGKAGPGSLHVVLQEA
                     TTAI"
     gene            4797..5318
                     /locus_tag="PK812_10045"
     CDS             4797..5318
                     /locus_tag="PK812_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69940.1"
                     /translation="MRQSRRAGRRRYPLGILAAVTPSKRRDLLRVLEDLRRSERFIRS
                     AISDLEQLLNPEASVPGRQPMASGVTFEAGFSEVAADYEADESMVRPPSAQGGSFAKW
                     SMSDAIQHVLEAAKGSLTNAEILAALTAGNFHFKTMQPAVAVAQCLKRLQDLGIVRRP
                     ERGQWLLAERTAS"
     gene            5395..>6667
                     /locus_tag="PK812_10050"
     CDS             5395..>6667
                     /locus_tag="PK812_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018237447.1"
                     /note="has catalase and peroxidase activities; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase-peroxidase"
                     /protein_id="HRJ69941.1"
                     /translation="MDGNEKNGNGKCPVAHAAPSSATFTGRSNRDWWPNQLNLSILHQ
                     HSPRSNPLTPAFNYAEEFKKLDYRALKNDLYKLMTESQDWWPADYGHYGPLFIRMTWH
                     AAGTYRTGDGRGGAGTGNQRFAPLNSWPDNGNLDKARRLLWPIKKKYGNRISWADLLV
                     LAGNAALESMGFKTFGFGGGREDIYAPEEDVYWGTEDTWLGDKRYSGDRDLENPLAAV
                     QMGLIYVNPEGPNGMPNAVASGRDIRETFGRMAMNDEETVALVAGGHTFGKAHGAGDP
                     ALVGREPEGAALEEMGFGWKNALGSGKGVHTTTSGIEGAWTPTPTTWDNSYFDMLFGY
                     DWNLVKSPAGAWQWIPASPAAQTLVPDAHDKTRRHPPMMTTADLALRMDPIYEPIARR
                     FHKNPDQFADAFARAWFKLAHRNMGPRVRYLG"
BASE COUNT         1205 a         2218 c         2123 g         1121 t
ORIGIN      
        1 ggccgcgccg aacggcaagc gggtcgcctg catcggcggt ggtccggcct cgctcaccgt
       61 tgcccgtgac ctcgccgtgc tcggctacgc ggtcgatgtg ttcgacgagg gcgccgcgct
      121 tggcggcatg atgcggtccc agattcccaa gttccgcctg ccggacgccg tcatcgacga
      181 agagtgcgcc tatatcaccg gcctcggtca ggtgacggtg caccggacgc gcgtcaactc
      241 gctgaaggag atgctgaccc ggggctatga tgccgtcttc gtcggcaccg gcgccccgcg
      301 cggcaagggc ctggacatcc ccgggcagga ggcgggcgcg tccaacatcc ataccggcat
      361 cgaatggctg gcgagcgtat cgttcgggca caccacgagc atcggacggc gggtgatcgt
      421 gctcggcggc ggcaacacgg cgatggattg ctgccggacc gcacgccgtc tcggcggcgc
      481 cgacgtcaag gtcatcgtcc ggtccggctt cgaggaaatg aaggcgtcac cgtgggagaa
      541 ggaggacgcg atccacgaag acattccgat cctcaatttc ctcacgccca aggcgttcgt
      601 gatccgtgat ggcaagctcg cgggcgtgac cttcgagaag gtgaaggcgg tctacgacga
      661 gaaggggcgc cgcagccttg ttcccaccgg cgagccggac gtcacgcatg actgcgatga
      721 tgttctcgtc gccatcgggc aggacaacgc cttcccctgg atcgagcgcg acgccggcat
      781 cgagttcgac cgctggggcc tgccgaagct cgacgaaaag acgttccgat cgaccctgcc
      841 gaacgtgttc ttcggcggtg atgccgcctt cgggccgaag aacatcatct gggccgtcgc
      901 gcatggccac gacgcggcgg tttcgatcca caaatactgc cagggcgagg atatcgcgga
      961 ccgcccgccg ccgggcgtct ccatcatcag ccagaagatg gggattcacg aatgggtcta
     1021 cgacaacgac atctcggtga aggcacgcac gccgatgccg catcgggcaa aggcggaagt
     1081 cctggccgac atccgcgccg aggtcgagct gggctttgac gccgctctgg cgctccggga
     1141 ggcgcagcgt tgcctcaatt gcgatgtgca gacggtgttt tccgcggcgc tatgcatcga
     1201 gtgcgatgcc tgcatcgaca tctgcccgat ggatagcctc accatcgcgc ccaacggcga
     1261 ggaaggcgat ctgcgcgcgc gcctcaccgc cccggcgctg aacctggccc aggacctgta
     1321 catctccgcc gatctcaaga ccggacgcat catggtgaag gacgaggacg tctgcctcca
     1381 ttgcggcctg tgcgccgagc gctgcccgac cggcgcctgg gacatgcaga aatacctgat
     1441 ccagatgacc cacgcggggt gcaagccgtg aacgtccatc ttcgccagcc ggtgaccgcc
     1501 accaacgatt tcgtcgtcaa gttcgccaat gtgaacggct ccggctcagc cagcgccaac
     1561 gagctgttcg cccggtcgat cctgcgcatg ggcgtaccgg tgtcgccgcg caacatcttt
     1621 ccctcgaaca tccaggggct gccgacctgg tacgaagtgc gggtttccgg aaaaggctgg
     1681 ctcggccggc gcggagggtg cgacatgatg gtcgcgatga acccgcagac ctgggcgcag
     1741 gacgtgaagg agatcgagcc gggcggctat ctcgtctacg attcgaccaa gccgatggga
     1801 ccggaagcct tccgcgacga catcaccgtc ataggtctgc cgctcaccgc gatttccaac
     1861 gccacgtatt cggacgtgcg ccagcgccag ctgttcaaga acatcatcgg cctcggcgcg
     1921 ctctcgctgc tgctgggcat cgatcccaag gcgatcgagc aactgttcgg cgagcagtac
     1981 aagggcaagg aggcgctgct cgcctcgaac gtcaaggcgc tgcatctggg acgggattat
     2041 gtcgagaaaa acctcgatcc caacatctgc cgcctgaagg tggtgcccgc caatgcggtg
     2101 ggagagcgca tcttcctcga aggcaacatg gcggcggcgc tcggctgcgt ctatggcggc
     2161 gcgaccgtgt gcgcctggta tccgatcacg ccctcgtcct cgctggccga ggccttccag
     2221 tcctactgca agcgctaccg gcacgatccc gacacgggcg aggcgaaata cgccatcgtt
     2281 caggctgagg atgaactggc ctccatcggc atggtgatcg gcgcggcctg gaacggcgcg
     2341 cgcgccttca cggcaacctc cggccccggc atttccctga tgaccgagtt catcggcctg
     2401 gcctactttg ccgaaatccc ggcagtgctg atcaacgtgc agcgcggcgg cccgtccact
     2461 ggcatgccga cccgcacgca acaggcggac atcctgtcct gcgcctatgc ctccaacggc
     2521 gacaccaagc acgtgctgct gttccccgag gatcccaccg agtgtttcga gatggcggcg
     2581 gatgccctcg atctcgcgga tcggctccag acgccggtat tcgtgatgac cgacctcgac
     2641 atcggcatga actaccggct gtgtcgcccg ctgaggtggg atgacagccg caagctcgat
     2701 cgcggcaagg tgatgacggc ggacgagttg caggccggca aagcgttcgg ccgctacacc
     2761 gacgtggacg gggacggcat tcccttccgc acctatcccg ggacccaccc gaccaagggc
     2821 gccttcttca cgcgcggcac ctccaagaac cgcaaggcgg gctattccga ggagggcgat
     2881 gtctacgtcg acaacatgga acggcttctg cacaagttcg aaaccgccaa gagcctgtgt
     2941 ccgcagccag aattccgtgc cgccgccaga ccgacgcgga ccggcgtgat ctacttcggc
     3001 tccaccgcgc cggccatgga tgaggcgctg gcccttctgg cggacgaagg ggcgcacttc
     3061 gatgcggtgc gcatccgcgc cttccccttc gcgtcctcga tcgatcgctt catcgccgat
     3121 cacgacaagg tcttcgtcgt cgaacagaac cgcgatgccc agttgcgctc catgctgatc
     3181 aacgagatcg gcgtcgaccc ggcgcggctg gtcgccgtca cgcactacga cggcacgccg
     3241 atcaccgcgc gcttcatcgt cggcgcgatc aacgccaacc tcgatctggc caggatccgg
     3301 ctcaatccgg agatgctgtc atgacctaca ttgccaagcc gaagctgcac catcccagcc
     3361 tgacgcgaaa cgaggcgggc tacacgcgcc gggattatga agggccggtg tcgacgctgt
     3421 gcgccggatg cggccacgat tcgatctccg cggcgatcat ccacgcctgc tacgagcttt
     3481 cgatcgaacc gcaccgggtg gcgaagctct cgggcatcgg ctgctcatcc aagacgccga
     3541 cctatttcct cggcaattcg cacgggttca acaccgtgca cggacgcatg ccctcggtgc
     3601 tcaccggcgc caacctcgcc aatcgcgacc tgatctatct cggcgtttcc ggcgacggcg
     3661 acagcgcctc catcggcctt ggccagttcg cgcatgtcat gcggcgcggc gtgaacatgc
     3721 tctacgtcgt catgaacaat ggcgtttacg gcctcaccaa gggccagttt tccgccaccg
     3781 ccgaccgggg atcgaagtcg aagaaggggg cggtcaacgc cgacagcgcc atcgatctgg
     3841 cctcgctggc gatcctgatg ggcgcgacct tcgtcgcccg ctcgttctcc ggcgacaagg
     3901 agcagctcgt ccccctgatc aaggcggcga tcagtcatcc cggcgcggcc atccttgacg
     3961 tgatctcgcc ctgtgtcgcc ttcaacaacc atgcgggttc caccaagagc tacgactata
     4021 tccgcgagca caacgaggcg gtgaaccgcc tcgactttat cacaggccgc gcgccaatca
     4081 cggcggacta tgcgccgggc gaggtgcgcg aaatcatgca gcacgatggc tccatgcttc
     4141 gcctgcgcaa gctcgatgcg acctatgatc cgtccagcaa ggccggcgcg ctcgcccata
     4201 tcgccgagcg tcaggcggcg ggagaggttg cgaccggact gctctatctc gatcccagtc
     4261 cgcgcgacct gcacgccgcg cagaacaccg tgaagcggcc gttgaacacc ctgacggaaa
     4321 aggacctgtg cccgggttcc gcagcgctgg aaaagatcaa cgccggattg cggtagaatg
     4381 ggctgccgca ccggcactga tgccgaaaat gcgagcgttt gggcttaccc ctgttgaacc
     4441 gtgcggccga aaatagcctg gttcggtctg gtttgttgag acatcgcagc gacgtgcgcg
     4501 cctagcatgc cttccgctca agggaggtgc actcatgccc agtgcgcgat ttttcttctt
     4561 ctcgctgctc gtggtttcca tcatgggatt ggtgttgccg accgacgcgc tggcctcctc
     4621 gggcaaggcg ggaccagggt ccttgcacgt cgtcctgcag gaggcgacga ccgccatttg
     4681 atgcgcttcc ggcgcgcggc agtcgccgcg accgcgcccg gtcaggtcgt gcgccaaggc
     4741 tttccgttgg cggcggagac ggtctgtggc atatccggtc ggtcgcctga atgggcttgc
     4801 ggcagagccg cagggcaggg cggcgccgtt atccgctcgg aattcttgcc gctgtgacac
     4861 cctcgaaacg tcgcgacctg ctccgcgtcc tggaagacct gcgtcggtcc gagcggttca
     4921 ttcgctctgc catctcggac ctggaacagc ttctgaatcc tgaagccagt gtcccaggcc
     4981 ggcagccaat ggcttcaggc gtgacgttcg aggcagggtt ttcggaggtc gcggccgact
     5041 atgaggcgga cgagtcgatg gtccggccgc cctcggctca aggcggcagc ttcgcgaagt
     5101 ggagcatgtc tgacgcgatt cagcacgtgc tcgaagctgc caagggaagc ctgaccaatg
     5161 ccgagatcct ggccgcgctc acggccggca actttcattt caagacgatg cagcctgcgg
     5221 tagcggtggc ccagtgcctg aagcgtctgc aggacttggg aatcgtgcgg cgtccggagc
     5281 gcggccaatg gctgctggcc gaacgaactg cctcttgagt ttgcccctcc gtcctctaag
     5341 ttgtttagaa gcgttctaag cgcgtcctca ggcctgcaac cagcgagatc gaccatggat
     5401 ggaaacgaga aaaacggcaa cggcaagtgc ccggtggcgc acgcggcgcc atcaagcgcc
     5461 acattcaccg ggcgctcgaa ccgcgactgg tggccgaacc aactgaacct gtcgatcctg
     5521 caccagcatt cgccgcgctc caaccctctg acgccggcct tcaactacgc cgaggagttc
     5581 aagaagctcg actacagggc cctgaagaac gacctgtaca agctgatgac cgagtcgcag
     5641 gactggtggc cagcggacta cggccattat ggcccgctgt tcatccgcat gacctggcac
     5701 gccgccggaa cctaccgcac gggcgacggc cgtggcggcg cgggcaccgg caaccagcgg
     5761 ttcgcgccgc tgaacagctg gccggacaac ggcaacctcg acaaggcgcg ccgattgctc
     5821 tggccgatca agaagaaata cggcaatcgc atctcctggg ccgacctgct ggtgctggcc
     5881 ggcaacgcgg cgctggaatc gatgggtttc aagaccttcg ggttcggtgg cggccgcgag
     5941 gacatctacg cgccggaaga ggatgtgtac tggggcaccg aggacacctg gctgggtgac
     6001 aagcgctact ccggcgaccg tgacctggaa aatccgctcg ccgccgtgca gatgggcctg
     6061 atctacgtga acccggaagg tcccaacggc atgccgaatg ccgtcgcctc gggccgcgac
     6121 atccgcgaga ccttcggccg catggcgatg aacgacgagg agaccgtcgc gctcgtcgcc
     6181 ggcggtcata ccttcggcaa ggcgcacggc gcgggcgatc cggcgctcgt tgggcgtgag
     6241 ccggagggcg ctgccctcga ggagatgggc tttggctgga agaacgcgct cggctcgggc
     6301 aagggcgttc acaccacgac cagcggcatc gagggcgcct ggaccccgac gcccacgacg
     6361 tgggacaaca gctacttcga catgctgttc ggatacgact ggaacctggt gaagagtccc
     6421 gccggcgcat ggcagtggat tccggccagc ccggcggcgc agacgctggt gcccgatgcg
     6481 catgacaaga cccgccgcca tccgccgatg atgaccacgg cggacctcgc cctgcgcatg
     6541 gacccgatct atgagccgat cgcgcgccgg ttccacaaga acccggacca gttcgcggat
     6601 gcattcgccc gcgcctggtt caagctggcc catcgcaaca tgggcccccg cgttcggtat
     6661 ctcggtc
//
LOCUS       DAOMXN010000145         6661 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_6063_length_6661_cov_11.042688, whole genome shotgun sequence.
ACCESSION   DAOMXN010000145 DAOMXN010000000
VERSION     DAOMXN010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 6661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6661
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6063_length_6661_cov_11.042688"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="PK812_10055"
     CDS             complement(<1..208)
                     /locus_tag="PK812_10055"
                     /inference="COORDINATES: protein motif:HMM:NF017757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRJ69942.1"
                     /translation="MKWSVEFLDQVVLDVLLELPSDIQASFVRIVGLIEGYGLEHVRE
                     PYVKHLEGPVWEMRMKGRDGIARAA"
     gene            complement(298..531)
                     /locus_tag="PK812_10060"
     CDS             complement(298..531)
                     /locus_tag="PK812_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003509408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRJ69943.1"
                     /translation="MAAAYLFGSTARGEARPDSDIDVFIEIDPNRKFSLIDMARVHNL
                     LMTELGVSVDLKTRSGLHPKLRAEIEREAVQAF"
     gene            complement(666..734)
                     /locus_tag="PK812_10065"
                     /pseudo
     CDS             complement(666..734)
                     /locus_tag="PK812_10065"
                     /inference="COORDINATES: protein motif:HMM:NF023398.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1018..1341
                     /locus_tag="PK812_10070"
     CDS             1018..1341
                     /locus_tag="PK812_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69944.1"
                     /translation="MAVTRQGDRTVVRVAPITHRWPGNGDRAMELPQQTKARLGLDGQ
                     RSWVILDHANEFVWPGPDVRPVPDRGPATIYFGPPPPAFYQALKLKLLNLQRVQRAQT
                     RLRGQ"
     gene            complement(1511..2350)
                     /locus_tag="PK812_10075"
     CDS             complement(1511..2350)
                     /locus_tag="PK812_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006471517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRJ69945.1"
                     /translation="MARIVCVGDNDVDCYLSDGRMYPGGNCFNVSVFARRSGVGSAYI
                     GAVGDDAAGRLIRKALESEGVDSSRLRVVKGDTAHCVIGHSGGDRQFVSYDLGVSRFE
                     PDEEDLQFIASHDAAHVYQHAGLDNCLGAIAARTRLSYDFSTRREASHRAEVAPRCWL
                     ASFSGAGQSREETIGLVGAMHATGAKWVLATRGSEGALLSGENTLHEVAAAPASVVDT
                     LGAGDSFIARVLVGLLRNETPKALLQAAAQDAARACEYFGAVGYGAPSTAWLQSSGGS
                     LQT"
     gene            2493..3494
                     /locus_tag="PK812_10080"
     CDS             2493..3494
                     /locus_tag="PK812_10080"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008840284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HRJ69946.1"
                     /translation="METSWRVDVAAALAALEVRPLTHVFLVACGGSLSVMLPGKVIMD
                     RFAPDLPCDALNAEEFICRAPARLGPAALVILCSQTGSTKETVAAAHFARGKGAATIA
                     MSLDIASPLAIAADYAVAYRAHYTTGEKIDAADSNYAVLYCLLAGLLAQRGQANLLAA
                     LRKSLAGLQPAIDRAKVALAEPLAAYAPRFANAPAVYTLGAGANFGAAYSFAICVLME
                     MQWINSQAIHANEFFHGPFEVVDKSACFIAMLGTGPSRRLEERARDFLLRYGDARNIM
                     VLDAAGLDLDGLEEPFREFLAPLVFFDVLWGFAFRLADARSQPMLEARRYMKKIANY"
     gene            3575..4576
                     /gene="dctP"
                     /locus_tag="PK812_10085"
     CDS             3575..4576
                     /gene="dctP"
                     /locus_tag="PK812_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007010205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HRJ69947.1"
                     /translation="MHFATITRRMLAGAAAVAAYAVTMGAVQAQTQIRIASVNPPTSE
                     SLVVVQKVADRITARTQGRVTFRLFPSGQLGTTKDSLEQASQGQPVITYTSASFMATF
                     GVPELAVVEGPFIVANNEEGERLAFSSLMQGWYDQLAAKSGIRIVALNWFDGPRHMIG
                     KAGYSKPDSLKGVRMRVPPVETWIKTFQPLGVIATTVEAAEAYSALSQGVVTAAESPL
                     TGLRASKWFEVAKHITLTGHFNLFTGWVMSEAAFKGLSADDRTALLDEFRKGGRELSD
                     LSLQQAAAIRKEFEAAGVTFHDADIEAYRKATASFYTSFPQWPAGLHEKVLAAAAGK"
     gene            4596..5081
                     /locus_tag="PK812_10090"
     CDS             4596..5081
                     /locus_tag="PK812_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008840282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HRJ69948.1"
                     /translation="MKALDDLLKAIGTFVPVVLLAVVLAIVPASVIARNLFDAEVYAA
                     HDIAMLAFAGVVWFGLVGAAIDGQLFGVNFFVDKLPGDLPRYCRIASCFVVILCSLAV
                     IYSAWMQIGTARFSRFLTLGWPKWIVSAGLLASMALIILVQLREIVLHCRGETAGGGA
                     P"
     gene            5078..6352
                     /locus_tag="PK812_10095"
     CDS             5078..6352
                     /locus_tag="PK812_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008840281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRJ69949.1"
                     /translation="MILATIVFFALLFFGVPVAFVMLGASVFYFIENPMMASIVAQRM
                     SSSLESFPLLAVPFFVFAGAAMARGGIADRLYDLAEGMVGHWRGGLAQVGVLNSLFMG
                     AMSGSANADAAIDARSLVPIMRRQGYSNAFGSVISAVSGVIAPIMPPSIGLIIYGLLT
                     NTSIGKLFLGGVVPAFLIAFVLMAAVRYVALRRNYAPSRERRLPLRDLAVRCRGALWA
                     LAMPVLLIVGLRVGWFTPTELGAMAALYALAIGLFVYRGFGVADVYDLLRESAYTTAS
                     VMFIVAAAGVLGVVLALEQVPQQIIGALLAISDNKYVVLVVINVALLVLGTVLEGLAL
                     LVILAPLLLKVTTSLGIDPVHFGVVLVFNTTIGSITPPVGTVLYTVCAITKCTIEEFS
                     VELVPFLIGLVGLLFLLTFFPPLVTFLPELMF"
     gene            6355..>6661
                     /locus_tag="PK812_10100"
     CDS             6355..>6661
                     /locus_tag="PK812_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008840280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HRJ69950.1"
                     /translation="MRLFDADAVHALLDYPGLIEALRRAHRGEGPDTAHMVRDDPAGG
                     ENKFVALVAWQNKSVITVKLVGVFPANLALAPPQPSVQGLVAVFDGATGAPVLAADGA
                     "
BASE COUNT         1127 a         2161 c         2066 g         1307 t
ORIGIN      
        1 aggcggcacg ggcgatgcca tcccggcctt tcatgcgcat ttcccagacc ggtccttcca
       61 ggtgctttac atagggctca cgcacatgct cgagcccata cccctcgatg aggccaacaa
      121 tccggacgaa gctggcctga atatcggaag gcagttccaa aagaacgtcg agaaccacct
      181 gatccagaaa ttcgactgac cacttcatac ccagattata ccttttttgc aatgaaacgc
      241 aacagacccc gaaatggggt gttgccgacg cctcctgatt ggaagcctga tgaattgtca
      301 gaacgcctga accgcttcgc gctcgatttc ggcacggagt ttaggatgca aaccagagcg
      361 ggttttcaga tcgacagaaa cgcccagttc cgtcatcagc aaattgtgta ccctggccat
      421 gtcgatcagg gagaatttgc ggtttggatc gatttcgatg aagacgtcga tatcactgtc
      481 ggggcgagcc tctccccgtg ccgtcgagcc gaagagatac gcggccgcca cccccgtgca
      541 cgcaatgcgg tttcgcggcg gccgatttca gcaagggcat ggtctcgctt catgagcaga
      601 ggatacacgg aattcgccgc catttgaagc tgcgtatcgt tcatccccac gagacaccac
      661 acgttctaaa caatccccca tgccctgtag cgttctcgcc ctgtgagttc gcgcaaaccg
      721 agatcggcga caaggtcctg cgccgcgcgt taggacatcc gcagcctgca ggcgaccaga
      781 gcggcgagat cagcggcggc tcgagggctg gagcgaggac gagattcatg cgctcgaagg
      841 catggcgatg gagcctggcc atgaggcgct gaacgccgaa ttcgaagcaa ccgggataaa
      901 aaggtgatag gctgcgcgcc caaacccggc ttcgtcatcc gctatctcca cgacttgtcc
      961 gcgccgggag aggtctcctc gccctccaac gtgaaggaac ggcctgcggt cgtcatcatg
     1021 gccgtgacgc gacaaggcga tcgaaccgtc gtacgcgtcg cgccgatcac gcatcgctgg
     1081 ccgggaaacg gcgatcgcgc tatggaattg ccgcagcaaa ccaaggcgcg cctcggcctc
     1141 gacggccagc gctcttgggt catcctcgat cacgccaacg aattcgtctg gcccgggccg
     1201 gacgtccgcc ccgtgccaga ccgcgggccc gcgacgatct actttggccc gccgccacca
     1261 gcgttctatc aagcgttgaa gttaaagctg ctgaacttgc agcgggtgca acgcgcgcaa
     1321 acgcgcctgc gcggacaatg agcgcccgaa gcggcttggg tcgttagcgg cgggagaatt
     1381 gcgtagccga tcaccggcaa ccctggggcg attcgattcc cggcgcaaag tcccgggttg
     1441 gacgaggcgg cgcgatgaca actctccgca ctcaaaaccg ggcgactgca gtcgaaacat
     1501 catccggcga tcatgtctgg agcgatccgc cagacgattg aagccacgcg gttgatggcg
     1561 ccccatatcc cacggcgcca aagtattcgc atgcccgggc ggcgtcctgc gccgcggcct
     1621 gaagcaatgc ctttggcgtc tcgttgcgca gaagcccgac caggacccgg gcgatgaaac
     1681 tgtcgcccgc accaagcgtg tcgactacac tggccggcgc ggcggcgacc tcgtgaagcg
     1741 tgttctctcc agaaaggagg gcgccctcgc tcccgcgtgt cgccagcacc catttcgcgc
     1801 cggtggcatg catcgcgccg acaaggccga tggtctcttc ccttgattgt ccagcgccag
     1861 agaacgacgc aagccagcag cgcggcgcca cctcggcgcg atggctggcc tcgcgccggg
     1921 tcgaaaagtc ataggagagg cgggttcgcg cggcgatcgc gccgaggcag ttgtccaatc
     1981 cggcgtgctg atacacatgg gctgcgtcgt ggcttgcgat gaattgaaga tcctcctcgt
     2041 ccggctcgaa ccgcgaaacg ccaagatcgt aagaaacgaa ttggcggtcg ccgccgctgt
     2101 gcccaatcac gcaatgcgcc gtgtcgccct tgacgacgcg cagacggctg gagtctacgc
     2161 cctcggactc cagcgctttc cggatcagcc ggcccgccgc gtcgtcgccg accgcgccga
     2221 tataggccga tcccactccg ctgcgccgcg cgaagaccga gacgttgaag cagttgccgc
     2281 ccggatacat gcgcccgtca gagaggtagc aatcgacgtc gttgtcgccc acgcatacga
     2341 tccgcgccat gcccgaactc acctctgtgc gaacttccgg attgacaaca tgtgagaacg
     2401 ttcgcacaat ggctctaggc cgtcaattca agctgcgaat gcgaaagtgc gggcggcacg
     2461 agtgtcgtga caggggcggg gcgaaaacgc tgatggaaac ctcttggagg gtcgatgtcg
     2521 cggcagccct ggcggcgctc gaggtcaggc ccctgactca tgtgttcctt gtggcatgcg
     2581 gcggttcgct ctccgtcatg ttgcccggca aggtgatcat ggatcgtttc gcgccggatt
     2641 tgccctgcga tgcgctcaat gctgaggagt tcatctgccg cgccccggca aggctcggcc
     2701 ctgcggcgct tgtcatcctc tgttctcaga ccgggtcaac gaaggagacg gtggcggccg
     2761 cgcactttgc gcgcgggaag ggtgcggcga ctatcgccat gtcgctcgat atcgcctcgc
     2821 cgctggccat cgccgcggac tacgccgtcg cttaccgcgc tcactacacg acaggcgaga
     2881 aaatcgacgc cgcggacagc aattacgccg tgctctattg cctgctcgct ggtcttctgg
     2941 cccagcgggg tcaggcaaat ctgctggccg cgctacgcaa gagcctcgcc ggccttcagc
     3001 ccgccatcga tcgggccaag gtcgcgctgg ccgagccgct cgcggcctat gcgccccgct
     3061 tcgcgaacgc ccccgccgtc tacacgttgg gcgcgggggc gaattttggc gcggcctatt
     3121 ccttcgcgat ctgcgtgctg atggaaatgc aatggatcaa ctcgcaggcc atccacgcca
     3181 acgaattttt ccacgggcca ttcgaggtcg tggacaagtc ggcctgcttc atcgcgatgc
     3241 ttggaacagg tccttcccgg cgtctggaag aacgggcgcg cgacttcctg ctgcgctacg
     3301 gggacgcgcg gaacatcatg gttctcgacg cggccgggct ggatctcgac gggctggagg
     3361 agcccttccg ggagtttctc gcgccgcttg ttttcttcga tgtgctttgg ggcttcgcct
     3421 ttcggctcgc cgatgctcgt agccagccga tgctggaggc gcgccgctac atgaagaaga
     3481 tcgccaacta ctaggtccgc tcccgccgga gcgaaccaga tcatgagtga accgcccgtc
     3541 acgggcagaa aaagcgcaag acaggaggaa ccacatgcat tttgcgacaa ttacacgccg
     3601 tatgcttgca ggtgcggcag ccgtcgcggc ctacgccgtc acgatgggcg cagtccaggc
     3661 gcaaacccag attcgcattg cctcggtcaa cccgccgacc tccgagtccc tagtcgtcgt
     3721 gcagaaggtg gcggatcgca tcacggcgcg cacgcaaggg cgcgtgacgt tccggctctt
     3781 cccgtccggt cagctcggca ccaccaagga ttcgctcgaa caggccagcc agggccagcc
     3841 ggtcatcacc tacacttccg cttcgttcat ggcgaccttc ggcgttcccg agcttgctgt
     3901 cgtcgagggg ccgttcatcg ttgccaacaa cgaggaaggc gagcgcctcg ccttttcgtc
     3961 gctcatgcag ggctggtacg atcagctcgc cgccaaatcc ggcattcgca ttgtcgcgct
     4021 caactggttc gacggtccgc gccacatgat cggcaaggcc ggctacagca agcccgacag
     4081 tctcaagggc gtgaggatgc gcgtgcctcc cgtggagaca tggatcaaga ccttccagcc
     4141 gctcggcgtc atcgccacga cggtggaggc tgctgaagca tattcggcgc tgtcccaggg
     4201 cgtggtgacg gctgcggaat cgcctctgac gggccttcgg gccagcaaat ggttcgaggt
     4261 cgccaagcac atcacgctga ccggccactt caaccttttc acaggctggg tgatgagcga
     4321 ggcggcgttc aagggtctct cggccgacga ccgcacggca ctcctcgacg agttccgcaa
     4381 gggcggccgg gagctttccg atctctcgct ccagcaggct gcggcgatcc gcaaggagtt
     4441 tgaggccgcc ggcgtaacct tccacgacgc cgatatcgag gcgtatcgca aggcgacggc
     4501 gagcttctat acgagcttcc cgcaatggcc ggccggcctg cacgagaagg ttctggcggc
     4561 ggccgccgga aagtgacgaa aggcgcggcc ggggcatgaa agcgctggac gatcttctca
     4621 aggcgatcgg gacctttgtc ccggtcgtcc tcttggccgt cgtgcttgcg attgtccctg
     4681 cgagcgtcat cgcccgcaat ctcttcgatg cggaagtcta cgcagcgcac gatatcgcca
     4741 tgctcgcctt tgccggagtg gtctggttcg gcctggtcgg cgcggccatt gacgggcagc
     4801 tctttggcgt caacttcttc gtcgacaagc tgcctggcga cttgccgcga tattgccgca
     4861 tcgcctcttg cttcgtcgtc atcctgtgca gcctggcggt catctattcc gcctggatgc
     4921 aaatcggcac tgcgcgcttc tcgcgtttcc tgacgctcgg ctggccgaag tggatcgttt
     4981 cggccggcct gcttgcgtcg atggcgctga tcatccttgt gcaactgcgc gaaatcgtcc
     5041 tgcattgccg gggcgaaacc gccggaggcg gtgcgccatg atcctcgcca cgattgtctt
     5101 tttcgcgctg ctctttttcg gcgttcccgt cgccttcgtg atgctcggcg cctcggtgtt
     5161 ctacttcatc gaaaacccga tgatggccag catcgtggcg cagcgcatgt cgtcgtcgct
     5221 ggagtccttt ccgctgctcg ctgttccctt ctttgtgttc gcgggggcgg cgatggcgcg
     5281 cggcggaatc gcggaccgat tgtacgacct cgcggaaggc atggtcggcc actggcgcgg
     5341 cggactcgcg caggtcggcg tcctgaattc gctgttcatg ggcgcgatgt ccggttcggc
     5401 caacgccgat gcggcgatcg atgcccgatc gctcgtgccg atcatgcgtc ggcagggtta
     5461 ctccaacgct ttcggctcgg tgatcagcgc ggtctccggg gtgatcgcgc cgatcatgcc
     5521 gccgagcatc ggcctgatca tctatggtct gctcaccaac acatcgatcg gcaagctttt
     5581 cctcggcggc gtcgttccgg ccttcctgat cgccttcgtg ctgatggccg cggtgcgcta
     5641 tgtcgcgctg cgccggaatt acgcgccgtc ccgcgagcgg cggcttccgc tccgcgatct
     5701 ggccgtgcgt tgtcgaggcg cgctctgggc gttggccatg ccggtcctgc tgatcgtcgg
     5761 cttgcgcgtc ggctggttca cgcccacgga gcttggcgcc atggcggcgc tctatgcgct
     5821 cgccatcggt ctgttcgtct atcgcggctt cggcgtcgcc gatgtctatg acctgctgcg
     5881 agagtccgcc tatacgaccg ccagcgtcat gttcatcgtc gccgccgcag gcgtccttgg
     5941 agtggttctc gcgctggagc aggtgcccca gcaaatcatc ggagcgcttc ttgcgatttc
     6001 cgacaacaaa tatgtggtgc tggtcgtcat caacgtcgcc ctgcttgtcc ttggcacggt
     6061 gctggaagga ctagccctgc tggttatcct ggcgccgctt ctcctcaagg tgacgacgag
     6121 ccttggcatc gatccagtgc atttcggcgt ggtgctcgtg ttcaacacga cgatcggctc
     6181 catcacgccg ccggtgggca ccgtgcttta cacggtctgc gccatcacca agtgcacgat
     6241 cgaggagttc tcggtcgaac tcgtgccgtt tctgatcgga cttgtcgggc ttctgttcct
     6301 gctcacgttc tttccgccct tggtgacctt ccttccagag ctgatgttct agccatgcgc
     6361 cttttcgacg ccgacgcggt tcacgcgctc ctcgactatc ccggcctgat cgaggcgctg
     6421 cgacgggcgc atcgtggcga gggacccgac accgcccata tggtgcgcga cgatccggcg
     6481 ggcggcgaga acaagttcgt cgcgctggtc gcctggcaga ataaatccgt gatcacggtc
     6541 aagctggtcg gcgtctttcc cgcaaacctt gcgcttgctc cgccccagcc ctccgtgcag
     6601 gggcttgtcg cggtcttcga cggagcgacc ggcgcgccgg tgcttgcggc ggacggggcg
     6661 g
//
LOCUS       DAOMXN010000146         6647 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_6080_length_6647_cov_9.948877, whole genome shotgun sequence.
ACCESSION   DAOMXN010000146 DAOMXN010000000
VERSION     DAOMXN010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6080_length_6647_cov_9.948877"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="PK812_10105"
     CDS             <1..448
                     /locus_tag="PK812_10105"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HRJ69951.1"
                     /translation="SLSAACERHQVPMVATELGGGARLDRSAFEIGLSGTRNLLRHAG
                     VLHGAPEATRTALHLTAGADAYLMAPVDGLFEGFHDLGTLIEAGQAAGRIWPMDDLTR
                     EPVPVSYGVTGRILCVRTMPMVRRGDFLLHTGAPMGDADFLETNPA"
     gene            445..1188
                     /locus_tag="PK812_10110"
     CDS             445..1188
                     /locus_tag="PK812_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HRJ69952.1"
                     /translation="MTASPIITLAGVNKWFGAFHVLKDIDLVVNRGEKVVVCGPSGSG
                     KSTLIRCINRLEEHDEGSIVVDGIVLDENVANVAAIRAEVGMVFQQFNLFPHLTVLDN
                     CVLAQVRARKTPKAEARQRAMQYLERVRIPEQAQKFPSQLSGGQQQRVAIARSLCMEP
                     KVMLFDEPTSALDPEMINEVLDVMSDLAQSGMTMICVTHEMGFARRVADRMVFMDHGR
                     IVETAPPKQFFSNPASDRTKLFLSQILSH"
     gene            complement(1205..2188)
                     /locus_tag="PK812_10115"
     CDS             complement(1205..2188)
                     /locus_tag="PK812_10115"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRJ69953.1"
                     /translation="MTAIDMHAHYYGGLVDDLMKRSTRPFVSRNGQGRLVLNAMTAST
                     EMSDGYTNLPARLAWMDRNGVATQLLTFPGALGVDVMPADEIAGPIRAFNDHLAAICA
                     GSGGRFAGLAGLPLADTALAAAEMRRVRRDLRLAVFTLPGNYFLSIAAARTLAPVLAA
                     ANECGALVMIHPGLMPGEAPPEPYPDTSVYRASVLNLQASLAQMGLTVIAGELAEAYP
                     RITFQLVNLGGTLPFVLERLEAVALSRPPYEPFPRAALRNMVYDCASLGPDALALAVK
                     VFGADRVMLGSDYPIFSPEALGAIAEKADISDAERALVLSGTAVRVLESLR"
     gene            complement(2280..2858)
                     /locus_tag="PK812_10120"
     CDS             complement(2280..2858)
                     /locus_tag="PK812_10120"
                     /inference="COORDINATES: protein motif:HMM:NF013720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit gamma family protein"
                     /protein_id="HRJ69954.1"
                     /translation="MTATRYAARVVALPMCALFEARGAEQGLRAALDAAGFAWPAHSG
                     VIVDGLHGSAILRFGPARVLIRADLSREAALADRLEPAFATVGDADVARVSDMYAVFQ
                     VAGSSAEDVLRQGAPLDLSADRLPQGSVVATELWAITAFISRDRTAEASFTLMVERSY
                     AGYVADWLAVANGGKPAASPGVMTNPPRPLAV"
     gene            complement(2855..5776)
                     /locus_tag="PK812_10125"
     CDS             complement(2855..5776)
                     /locus_tag="PK812_10125"
                     /inference="COORDINATES: protein motif:HMM:NF013720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRJ69955.1"
                     /translation="MTAMRLPNGGLVDRTQPLRFQFDGMEITGFAGDTIASALLANDI
                     VLAGRSFKLHRPRGFVGAGYDDGIMVDRLSPRPAPNQLAAVTGIEPGAVYRSVSTWPS
                     ARRDFGAVAGFFAPLLPAGFYYKTFMRPSWHLFEPLIRAAAGLGRVPVETWQPTSESR
                     YGDCDVLVVGAGPAGLAAASTAGASGARVMLVDDQPSPGGRLLHDGFADQAWLAEMLA
                     MLEAMPNVRRLPQSSVWAYHEHNLLTVIERAPSDARDLDFRTWKVRARQVVIAAGAQE
                     RPIPFPDSDRPGVMLASAARSYLHRHGVRVGARAIVFTNNDSAYATALDLAQAGSAVT
                     VLDARPTVAPEVQARAADRGIRLRTGAQVVRAIGRARVSALEFETAQGRERLDCDLVA
                     VSGGWNPAFHLASQSRQATSVWRPDIATFVARHASEDFSIAGAATGTFGTSDCAREGA
                     AAAATALGRLGIKSPAVPVPESGDAFVANEVHPLWFVPRSKGAGKVFVDLSGDVSTDD
                     LGLAMREGYDSIELIKRYTTAGMGVDQGKTGNVNVIAVVGALSGQPADAVGTTTFRPP
                     YVPVEFGAIAGARSQARLYPWRHTPLTGWHVEQGAVMYEAGLRWQRPGYYPQPGEDWS
                     AAAQREARAVREGVGVYDGTPLGKFQLKGPDVPRLLDLAYVGDFSKLKPRRGKYSVMM
                     SDDGLILDDGVTFRLDEHRWLLHSSTGAADRVHGHLEQLLQVHRPDWRVSLIPVTTQW
                     ANATLCGPLARDVLAALNPDFDISNKALPFMGMVEGHVAGLPVRVFRVSWTGELSFEL
                     NTPARHGPALWQAVMQAGARFAITPVGSEANHILRVEAGYLSTGHEVDGTADVHDLGL
                     SGLIGPAKTDFLGKRSMEIRRRADPSRFELVGLLPEQGQMVPEGAPITPGGDRIDQEG
                     FVSACVMSVAQQRPVALGLLREGRRRMGETVRARVRDRIIAMQVVEPVFHDASRSRVK
                     S"
     gene            complement(5773..6030)
                     /locus_tag="PK812_10130"
     CDS             complement(5773..6030)
                     /locus_tag="PK812_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HRJ69956.1"
                     /translation="MLLIDCPFCGKRPEGEFSCLGEAVAPRPDPAGLDDDTWSAWLVG
                     RENRRGFHSERWWHVRSCGRVFTITRDTVTHAIQPHAEAGQ"
     gene            complement(6035..>6647)
                     /locus_tag="PK812_10135"
     CDS             complement(6035..>6647)
                     /locus_tag="PK812_10135"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRJ69957.1"
                     /translation="RAPDGRVTGVETNRGVVRARCIALATAGNTSHVAARAGLTLPLV
                     TRNLQAFVSEPVKPIIDVIVNCPDVGLYLMQSDKGEIVIGGGTDPLPSYRQGCHFSTF
                     EDVVASLIELFPRFKSLKMLRQWGGAIEFAYDASPIISATPIDGLYVSTGWYGGFKSI
                     PVGGMTFAHLIATGRPHPLAEAFGLERFPALKFLLEAGTVAQR"
BASE COUNT         1187 a         2256 c         2168 g         1036 t
ORIGIN      
        1 gtcgctttcg gcggcctgcg agcgccacca ggttccgatg gtcgccaccg agcttggcgg
       61 cggcgcgcgg cttgaccgaa gcgccttcga gatcggcctg tccggcacgc gcaacctgct
      121 gcgtcatgcc ggcgtgctgc acggcgcgcc cgaggccacg cggacggcgc tccatctcac
      181 cgccggcgcc gatgcctatc tgatggcgcc ggtcgacggt ctgttcgaag ggtttcacga
      241 tcttgggacc ctcatcgagg ccgggcaggc agccgggcgc atctggccga tggatgacct
      301 gacgcgcgag cctgttccgg tcagctatgg cgtgaccggg cgcatcctgt gcgtgcgcac
      361 catgccgatg gtcaggcgcg gcgatttcct gctgcatacg ggcgcgccca tgggcgatgc
      421 cgatttcctg gagaccaacc ccgcatgact gccagcccca tcatcacgct tgccggcgtc
      481 aacaagtggt tcggtgcctt ccatgtgctg aaggacattg atctcgtggt gaatcgtggg
      541 gagaaggtcg tggtctgcgg tccctccggc tccggcaagt cgacactgat ccgatgcatc
      601 aatcgcctcg aagagcacga tgaagggtcg atcgtggtgg acggcatcgt gctggacgag
      661 aacgtcgcca acgtcgcggc gatccgcgcc gaggtcggca tggttttcca gcaattcaat
      721 ctgtttccgc atctgacggt gctggacaat tgcgtgctgg cgcaggtgcg ggcgcgcaag
      781 acgcccaagg ccgaggccag gcaacgcgcg atgcagtatc tggaacgggt ccgcatcccc
      841 gagcaggccc agaaatttcc ttcgcagctg tccggtggcc agcagcaacg cgtcgccatt
      901 gcccgttcgc tgtgcatgga gcccaaggtc atgctgtttg acgagccgac gtccgcgctc
      961 gatccggaga tgatcaacga agtgctggac gtgatgagcg accttgccca gtccggcatg
     1021 acgatgatct gcgtcacgca cgaaatgggc ttcgcgcggc gtgtggcgga ccgcatggtg
     1081 ttcatggatc atgggcggat cgtggaaacc gcgccaccca agcagttctt ctcgaacccc
     1141 gcctccgacc ggacgaagct gttcctgagc cagattctgt cccactgacc gcgaagcgaa
     1201 cgcgctatcg cagactttca agcacgcgaa ccgccgtgcc ggacagcacc agcgcccttt
     1261 cggcatcgct gatgtctgcc ttttctgcga tggcgcccag cgcctccggc gaaaaaatcg
     1321 ggtagtcgct gccaagcatc acgcggtcgg cgccaaacac cttgaccgcc agcgccagcg
     1381 catccggccc gagcgaggcg cagtcgtaga ccatgttccg cagcgcggcg cgcgggaaag
     1441 gttcgtatgg cggacgcgaa agcgcgaccg cttccagccg ttccagcaca aagggcagcg
     1501 tgccgccgag attgaccagt tgaaacgtga tgcgcggata ggcctcggca agctccccgg
     1561 cgatcaccgt cagtcccatc tgcgcgaggc tcgcctgaag gttcagcacg gacgcgcgat
     1621 agactgatgt atccgggtaa ggttcgggcg gcgcttcgcc cggcatcagt ccggggtgga
     1681 tcatcacaag cgcgccgcat tcgttcgccg ccgcaagcac gggggcaaga gttcgcgctg
     1741 cggcgatcga caggaagtag ttgcccggca gggtgaagac cgccagccgc aagtcgcgcc
     1801 ggacgcgccg catctccgcc gccgccaggg ccgtgtcggc gagcggcagc ccggcaagac
     1861 cggcgaagcg gccgccggac ccagcgcaga tcgcggcgag gtgatcgttg aacgcccgga
     1921 tcggcccggc gatttcgtca gccggcatca catcgacgcc aagcgcgccg ggaaaggtca
     1981 ggagttgcgt ggcaactccg ttgcgatcca tccaggccag ccgggccggc aggtttgtgt
     2041 agccgtcgga catctcggtc gaggccgtca tggcgttgag caccagccgg ccctgcccgt
     2101 tccggctgac gaaggggcgc gtgctgcgct tcatcaggtc atcgaccaga ccgccgtagt
     2161 aatgtgcgtg catgtcgatc gcggtcatgc cgttctccgt cgacgagtca ggaaaaggga
     2221 tctctcacag acctgaggcc tggccgatgc cccacgagac cagattcggc cggagccagt
     2281 cataccgcaa gggggcgcgg tggatttgtc atgactccgg gcgatgcagc aggctttcca
     2341 ccattggcga ccgccagcca atccgcgaca tagccggcgt aggacctttc gaccatgagc
     2401 gtgaaggatg cctcggctgt ccggtcgcga ctgatgaacg cggtgatcgc ccaaagctct
     2461 gtcgcgacaa cactgccctg tggcagcctg tccgcagaca ggtccagcgg cgcaccctgg
     2521 cgcagcacat cctccgcgct ggaacctgcc acctggaaca cggcatacat gtcggagacc
     2581 cgggcgacat cggcgtcgcc caccgtcgcg aatgcgggtt ccagccggtc ggccaacgcc
     2641 gcttcccgcg aaaggtccgc ccggatgagc acgcgcgccg ggccaaagcg aaggatggcg
     2701 ctgccgtgca ggccgtcgac gatcacgcca gaatgcgcag gccacgcgaa tccggccgcg
     2761 tcgagcgcag cgcgcaagcc ctgctctgca ccgcgcgcct cgaacagcgc gcacatcggc
     2821 aacgcaacaa cgcgggccgc gtagcgtgtg gctgtcatga cttcacccgc gaacggctgg
     2881 catcgtggaa caccggctcg acaacctgca tggcgatgat cctgtccctg acacgagcac
     2941 gcacggtctc gcccatccgc ctgcgacctt cccgcagcag gccgagcgcc accggacgtt
     3001 gctgcgcgac gctcatgacg caggccgaaa cgaacccttc ctgatcgatg cgatcgcccc
     3061 cgggtgtgat cggcgcgcct tcgggcacca tctgtccttg ctccggcagc aaaccgacca
     3121 gttcgaaacg cgacgggtcg gcccggcggc ggatttccat ggaccgcttg ccgaggaagt
     3181 cggtcttggc cgggccgatc aggccggaca acccgagatc atgcacatcc gccgtgccgt
     3241 cgacctcgtg gccggtcgaa aggtagccgg cttcaacccg taggatatgg ttggcctccg
     3301 agccgaccgg cgtgatggcg aagcgtgcgc ctgcctgcat cacggcctgc cacagcgccg
     3361 gtccgtgccg cgccggggta ttcagctcga agctgagttc gcccgtccag ctcacgcgga
     3421 agacgcggac cggcaggcct gcgacatgcc cttcgaccat gcccatgaag ggcagcgcct
     3481 tgttcgagat atcgaaatcg ggattcaagg ccgccagaac gtcgcgtgcg aggggaccgc
     3541 atagggtggc gttggcccat tgcgtcgtca cgggaatgag actgacgcgc caatccggtc
     3601 gatggacttg caacagttgt tccagatgtc cgtgcaccct gtccgccgct ccggtcgagg
     3661 agtggagcag ccagcgatgc tcatcgagcc ggaaggtcac gccatcatcg agaatgagcc
     3721 catcgtcgct catcatcacg gagtacttgc cacgtcgcgg cttgagtttg gagaagtcac
     3781 cgacataggc gagatcgagc aggcgaggaa cgtcgggccc cttgagctgg aacttgccga
     3841 gcggcgtgcc gtcatacacg cccaccccct cgcgcacggc ccgggcctcg cgctgcgccg
     3901 cggcggacca gtcttcgccc ggctgcggat agtaccccgg gcgctgccat cgcagccccg
     3961 cctcatacat caccgcgccc tgctccacat gccacccggt aagcggcgta tggcgccaag
     4021 gatagagacg cgcttgcgac cgcgcgccgg caatcgcgcc gaactccacc ggaacatacg
     4081 gcgggcggaa cgtggtcgtg cccaccgcgt cggccggctg cccgctcagc gctcccacga
     4141 cggcaatcac attgacattg ccggtcttgc cctgatcaac acccatcccg gctgtggtgt
     4201 agcgcttgat cagctcgatc gaatcgtagc cctcgcgcat cgccagcccc aggtcgtcgg
     4261 tgctgacgtc gccggacaga tcgacgaaca ccttgcccgc tcctttggag cgcgggacaa
     4321 accaaagcgg atggacctcg ttggcgacaa acgcatcgcc gctctcaggc acaggcacgg
     4381 caggtgactt gatgccaagt cggccaagcg ccgtcgcggc cgccgccgcg ccttcccgcg
     4441 cgcagtccga tgttccgaag gtgccggtcg cagcgccggc gatggaaaaa tcctcagacg
     4501 cgtggcgggc gacgaacgtg gcgatgtccg gccgccagac agaggtggcc tgccggctct
     4561 gcgaggcgag atggaacgca gggttccagc cgccgctgac ggcgacgaga tcgcaatcga
     4621 gccgttcgcg gccctgcgca gtctcgaact ccagggccga cacgcgggcg cggccgatgg
     4681 cgcggacaac ctgcgcgccg gtccgcagac gaatgccacg atccgccgcc ctggcctgca
     4741 cttccggcgc gactgtcggg cgagcatcaa gcaccgtgac ggccgagcct gcctgagcca
     4801 gatcgagcgc ggtggcatag gcggaatcgt tgttggtgaa gacgatggcc cgcgccccga
     4861 cgcggacgcc gtggcgatgc agatagctgc gcgccgcgga cgccagcatg acgcccggcc
     4921 ggtcactgtc cggaaaagga atcggccgtt cctgcgcgcc ggccgcgatc acgacctgac
     4981 gcgcgcggac cttccaggtg cggaagtcga ggtcgcgcgc atccgatggc gcgcgttcga
     5041 tcacggtcaa caggttgtgt tcgtgatagg cccagaccga tgactgcggc agtcgacgga
     5101 cgttgggcat cgcctccagc atcgccagca tttccgcgag ccaggcctgg tctgcgaagc
     5161 cgtcgtgcag caaacggccg cctggacttg gctggtcatc cacaagcatc acgcgcgcgc
     5221 ccgacgcgcc tgccgtcgag gctgcggcaa gaccggccgg acctgccccg accaccaaca
     5281 cgtcgcagtc accgtagcgg ctctcgctcg tcggctgcca ggtttcgacc ggaacgcggc
     5341 ccagcccggc tgcggcccgg atcagcggtt cgaacagatg ccaggacggc cgcatgaagg
     5401 tcttgtagta gaatcccgcc ggcagcagcg gcgcgaagaa acccgcgact gcgccgaagt
     5461 ccctgcgcgc gctcggccag gtcgaaacac tgcgatacac cgcgcccggc tcgatcccgg
     5521 tgacagccgc caactggttc ggcgccggtc tgggcgacag ccggtccacc atgatgccat
     5581 catcgtagcc ggcgcccaca aagcctcgcg gacgatgcaa cttgaagctg cgccccgcga
     5641 gcacgatgtc gttggcaagc agcgccgagg caatggtgtc gccggcaaag ccggtaatct
     5701 ccatgccatc aaactggaac cgcaggggtt gcgtgcgatc gaccagaccg ccatttggca
     5761 ggcgcatcgc cgtcattgac ccgcctccgc atgcggttga atcgcgtggg tgaccgtgtc
     5821 acgcgtgatc gtgaacaccc ggccgcagct tcggacatgc caccagcgct cactgtggaa
     5881 gccgcgccgg ttctcgcgcc cgacgagcca ggccgaccag gtgtcatcgt cgagtccggc
     5941 gggatcggga cggggcgcga ccgcttcgcc aagacaggaa aattcgcctt cgggacgctt
     6001 tccgcaaaag ggacagtcaa tcagcagcat cgcctcacct ctgtgccacg gtgccggctt
     6061 ccagaaggaa tttcagcgct gggaagcgtt ccaggccgaa ggcctcagcc agcgggtgcg
     6121 gccggcccgt ggcgatcagg tgggcgaacg tcattccacc gacggggatc gacttgaagc
     6181 cgccgtacca gccggtgctc acataaagcc cgtcgatcgg cgtggccgag atgatggggc
     6241 ttgcatcata ggcgaattcg atcgccccgc cccactggcg cagcatcttc aggctcttga
     6301 aacgcgggaa cagctcgatc agcgaggcaa ccacgtcctc gaaggtcgag aagtggcacc
     6361 cctgacggta ggacggaagc gggtccgtgc cgccgccgat aacgatctcg cccttgtcgg
     6421 actgcatcag gtagaggccg acatccggac aattgacgat cacgtcgatg atcggcttga
     6481 ccggttccga gacgaaggcc tgcaggtttc gcgtcaccag cggcagggtc aagccggcgc
     6541 gcgcggcgac atggctcgtg ttcccggcgg tcgccagcgc aatgcagcgc gccctcacga
     6601 cgccgcggtt ggtctcgacg cccgtcacac gcccatcggg cgcccgc
//
LOCUS       DAOMXN010000147         6492 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_6275_length_6492_cov_9.844648, whole genome shotgun sequence.
ACCESSION   DAOMXN010000147 DAOMXN010000000
VERSION     DAOMXN010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 6492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6492
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6275_length_6492_cov_9.844648"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..133)
                     /locus_tag="PK812_10140"
     CDS             complement(<1..133)
                     /locus_tag="PK812_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003597539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HRJ69958.1"
                     /translation="MTDLSKLVDDLSKLTVLEAAELAKMLEEKWGVSAAAAVAVAAAP
                     "
     gene            complement(192..710)
                     /gene="rplJ"
                     /locus_tag="PK812_10145"
     CDS             complement(192..710)
                     /gene="rplJ"
                     /locus_tag="PK812_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HRJ69959.1"
                     /translation="MDRAGKKELVSTLNSAFKNTGSIVVAHYSGLTVAQMQKLRREMK
                     KSGATVKVAKNRLVKIALDGTDVASIGSLMKGPTLLAFAKDPVAAPKVAVEFAKANDK
                     LVILGGAMGTTALKPDGVKALATMPSLDELRAKLVGLIQAPATKIAQLSTAPAAKLAR
                     VFGAYAKRDEAA"
     gene            complement(1285..1737)
                     /locus_tag="PK812_10150"
     CDS             complement(1285..1737)
                     /locus_tag="PK812_10150"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRJ69960.1"
                     /translation="MSEAVTVRPLQAADRADWERLYQGYAAFYRVEQTAEMRARVWGW
                     LMDEGHEVKGLVAVGAGGALIGLAHIRPFARPLSATVGGFLDDLFVDPAARGSGAADA
                     LFAAIKAEGQARGWSVIRWITADDNYRARAVYDRLAERTKWVTYDMKV"
     gene            complement(1734..2543)
                     /locus_tag="PK812_10155"
     CDS             complement(1734..2543)
                     /locus_tag="PK812_10155"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRJ69961.1"
                     /translation="MFNYKKTLNLADREVVLTFDDGPMPRRTPAVLDALEAECVKATF
                     FVVGSMAAAYPDILQSTARAGHTIGTHTWSHRYLNRTKAQKTREEQIAGGLHATNIAL
                     GGENRAQLSPLFRFPGLGRTRALDRFVEANGLLSMSVDVVSDDWRRISPDEVLKRTLA
                     RLDARGKGILLLHDIQPRTVAMLPDLLKELKARGYRIVHVTADAKETEVALANLAEPQ
                     GRGFQVAMARTRQKLEVLTAQASAGPGQSFLAGSRSLKVAVKGFEELGLRR"
     gene            complement(2776..3450)
                     /locus_tag="PK812_10160"
     CDS             complement(2776..3450)
                     /locus_tag="PK812_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003501589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HRJ69962.1"
                     /translation="MLSLRACAAAILLALTPVAQAQTPPSASEIAAYTGLHAAAHKGD
                     AAEIARLIAAKADPNGRDGAGRTPLHVATFARKHEAMRALAKGGADPRAQENRKYDTV
                     TIAAVADDLATMKVALEIGGSAKDITSVYTGTALIAAAHLGHVEVVQELIRAGAPLDH
                     INNLHWTALMESVVLGDGGPRHTATLKALVDAGARRDLADREGRTPLDHARARGYAEM
                     VRLLEK"
     gene            complement(3619..4308)
                     /gene="rplA"
                     /locus_tag="PK812_10165"
     CDS             complement(3619..4308)
                     /gene="rplA"
                     /locus_tag="PK812_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HRJ69963.1"
                     /translation="MVAKRVAKSREGIDRTKLYPLGEAVKLIKDRASAKFDETVEIAL
                     NLGVDPRHADQVVRGVCNLPNGSGRTVRVAVFARGPKADEAKAAGADIVGAEDLFEKV
                     NGGTIEFDRCIATPDMMGLVGRLGKVLGPRGLMPNPRVGTVTMDVTAAVKGAKGGSVE
                     FRAEKAGIVHGGVGKISFTAEKLEQNIRAFADAVIKAKPTGAKGQYLLRASITSSMGP
                     GVKVDLASLHG"
     gene            complement(4312..4740)
                     /gene="rplK"
                     /locus_tag="PK812_10170"
     CDS             complement(4312..4740)
                     /gene="rplK"
                     /locus_tag="PK812_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HRJ69964.1"
                     /translation="MAKKIVGFIKLQVPAGAANPSPPIGPALGQRGLNIMEFCKAFNA
                     KTQQMEKGTPIPVIITAYQDRSFTFEMKQPPVSFFIMKAIGLKAGSKTPGRGFVGKIT
                     KKQIAEIAEKKMADLNCDSVESASAMIAGSARSMGVEVVG"
     gene            5001..>6492
                     /locus_tag="PK812_10175"
     CDS             5001..>6492
                     /locus_tag="PK812_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69965.1"
                     /translation="MIGEAALLKILGAGVAFGAKAYKDIAGLKAEDFSEVAKSLGEAF
                     IGGKPVSGDMTAFISGVQRSIADTLDQRTMAEAGRNPEDVRRDRENALVQFNETLPKM
                     ALDRALFARAEFDAYAVATAMTDQLARLNPLFRPPASGAPRSIAYALACEIFFRTFEL
                     AEQKKEFCETLLATIHREVLKRLGRIEDNTSRTLDELADIKAQLKALALAKGVPEEPL
                     RKVLARFGEEHVPLGDIPDRLARKADELVFLRQELARLRNDRPEFAALRARALKLIDD
                     GEFDEARAELRKGRELAKAIREEHAREEASFLRHEARIDRLNLDYAAAIAAYRQATAL
                     DPDNLPAWGELGDLWIILGNLTDALKAFEGAEAACIRSGDERNLSVSHDRIGDVRVAK
                     GDLAGALESYTASLAIRQTLADADRTNSGWQRDLSVSHKKIGDVRVAKGDLAGALESY
                     IASLAIRQTLADADRTNSGWQRDLSVSHKKIGDVRVAKGDLAGALES"
BASE COUNT         1133 a         2203 c         2039 g         1117 t
ORIGIN      
        1 ccggagccgc ggcgacggcc acggcggcgg cggcggagac gccccacttc tcttccagca
       61 tcttggcgag ttcagcggct tcgagaacgg tgagcttcga caggtcgtcg acgagcttgc
      121 tcaggtcggt catgggtttg ttccttgata tgggtatctg tttgaacgtt tcgattgtct
      181 gtcaggcggc atcacgccgc ttcgtctcgc ttggcatagg ccccgaacac gcgcgcaagc
      241 ttggcagccg gcgcggtcga cagctgcgcg atcttggtag ccggggcctg gatgaggccg
      301 accagcttgg cgcgcagttc gtcgagcgac ggcatggtgg cgagcgcctt cacaccatcg
      361 ggcttcaggg cagtggttcc catcgcgccg ccgaggatca cgagcttgtc gtttgccttg
      421 gcgaattcga cggcaacctt cggcgccgcg accgggtcct tggcgaaagc caggagcgtc
      481 ggtcccttca tcagggaacc gatggacgcg acatccgtgc catcgagagc gatcttgacg
      541 aggcggttct tcgcgacctt cacggtggcg ccagacttct tcatctcgcg acgaagcttc
      601 tgcatctggg caacggtgag gcccgaataa tgcgcaacga cgatggaacc ggtgttcttg
      661 aacgccgagt tcaacgtcga gacgagctct ttcttaccag ctctgtccac gtgctctctc
      721 cgggttgaca ggttcggaac acgaacccgt cggttgcaac tgccgacccg acaggggccc
      781 gggaacgggc ttccggcggg gcggcgcctg aatgggatta cggcggcaag ccaccacaac
      841 cccgctgttg cctgtccccc cggatgtggc gaaacgcgcc agacccaagg acaaatggtt
      901 caaaccaaac acaccgcacc ctctcaacga gggcaaaagc atccggtctt ccccgtctcc
      961 tgcggacgcc ttgaccggat cgcacccggc gggaaaatcg ctcttccctg gccttaagcc
     1021 ggcttgaagt tcccttcagc ccgacgcatc cgccgtcttg gacaggacat ggccggaaaa
     1081 gacaacaggt caccctgcca tctcccggcc cttcctccgc tcaccgaccc cttgcaggcc
     1141 cggcggcggt aactcgtgat ttgcgcgcaa taccggcgcg cgcggacagc atgctgctga
     1201 tcatggcgcg ctctatatcg ggcaaccgcc agagtcgcaa ggggcgttgt caaggaattt
     1261 ttcagcgccc cccgcgccag caacctacac cttcatgtcg taggtgaccc acttcgtccg
     1321 ctcggccagc cggtcataga cggcgcgggc gcgatagttg tcatccgccg tgatccagcg
     1381 gatcaccgac catccgcgcg cctgtccctc ggccttgatg gcggcgaaca atgcatcggc
     1441 agcgccgctg ccgcgcgcgg caggatcgac aaagagatcg tcgagaaagc cgccgaccgt
     1501 tgcagacagc ggccgcgcga acgggcggat atgggcaaga ccgatcagcg cacccccagc
     1561 cccaaccgcg accagcccct tgacctcatg cccctcgtcc atcagccagc cccagacgcg
     1621 cgcccgcatc tccgccgtct gctcaaccct gtaaaacgcc gcatagccct gatagagccg
     1681 ctcccaatcc gcccggtcgg ccgcttgcag cggacgcacc gtcaccgctt cgctcatcgt
     1741 ctcaacccca gttcctcgaa ccccttcaca gccaccttca ggctgcggct gccggcgaga
     1801 aagctctggc ccggccccgc gctggcctgc gccgtcagca cttccagctt ctgccgggtg
     1861 cgggccatcg ccacctggaa gcccctgccc tgcggctcgg cgaggttggc gagcgccacc
     1921 tcggtctcct tcgcatcggc ggtgacatgc acgattcgat agccccgggc cttcagctcc
     1981 ttcagaaggt cgggcagcat cgccacggtg cgcggctgga tgtcgtgcag cagcaggatg
     2041 cccttgccgc gcgcatcgag ccgggccagc gtgcgcttca gaacctcgtc gggcgaaatc
     2101 ctgcgccagt cgtcgctgac cacatccacc gacatcgaga gcagcccgtt cgcttcgacg
     2161 aaacgatcaa gcgccctggt gcggccgagg cccgggaagc gaaacagtgg tgaaagttgt
     2221 gcccggtttt cgccgcccag cgcgatgttc gtggcgtgca acccgccggc aatctgctcc
     2281 tcgcgcgttt tctgcgcctt tgtgcggttg agatagcggt gcgaccaggt gtgcgtgccg
     2341 atcgtatgcc cggcccgtgc ggtggattgc agaatgtcgg gataggccgc cgccatcgag
     2401 ccgacgacga agaacgtcgc cttcacgcat tcggcctcca gcgcatcgag caccgcgggc
     2461 gtgcgccggg gcatcggccc gtcgtcgaaa gtcagcacca cctcgcggtc ggcaagattg
     2521 agcgtctttt tgtagttgaa cacgccgacc ggaccatcgg cagccgataa tttcagggtc
     2581 cggtgcgtac ccagcgcctc cggcccgcat gcagccgccg aggcgccgca tgacatcatc
     2641 actccggccg caagcgcggc cccatacgca aactgacgca acgctccgcc cccgggataa
     2701 cgcaacacta aagggccccg agcgtggcag cttatggttg ataaagtgct gacggcggat
     2761 catgccggcg tgaggttact tctccagcag ccgaaccatc tccgcatagc cgcgtgcgcg
     2821 ggcatgatcg agcggcgtgc ggccctcgcg gtcggcgaga tcgcggcggg cgcctgcgtc
     2881 caccagcgct ttcagcgtcg cggtatggcg tgggccaccg tcgcccagca ccaccgattc
     2941 catcagcgcc gtccaatgca ggttgttgat gtgatcgagc ggcgcgccgg cgcggatcag
     3001 ctcctgaacc acctcgacat ggccgagatg cgccgccgcg atcagcgccg tgccggtgta
     3061 gaccgaggtg atatccttcg cgctgccgcc aatttccagc gccaccttca tcgtcgccag
     3121 atcatcggca acggcggcga tggtcacggt gtcgtatttg cgattttcct gcgcacgcgg
     3181 atcggcacca cccttcgcca gcgcccgcat cgcctcatgc ttgcgggcaa aagtcgccac
     3241 atgcaagggc gtgcgccctg ccccgtcgcg cccgttcgga tcggccttcg ccgcgatcag
     3301 cctcgcgatt tccgccgcat cgcccttgtg ggcggcggca tgcaggcctg tataggcagc
     3361 gatttcggag gccgatggcg gggtctgcgc ctgggcaaca ggcgtcaggg ctaggagaat
     3421 agcggcggca caggcgcgga gtgacaacat tgatcggcct tggatcatga caacgcgccg
     3481 accttacctg ctgccggcct cacgctcacc caaaatcctc gtgaacgggc gcccgcccaa
     3541 tgccacgtcg gcgccataga aaaaccccgg cgtttccgcc ggggttctga actcagtcgg
     3601 ggagcccgag gggcccgctc acccgtgcag gctggcgaga tcgaccttga cgcccggccc
     3661 catcgacgag gtgatggagg cgcgcaggag atactggccc ttggcgccgg tcggcttggc
     3721 cttgatcacc gcatcggcga aggcgcggat gttctgctcc agcttctcgg cggtgaagga
     3781 gatcttgccg acgccgccgt ggacgatgcc ggccttttcg gcgcggaatt cgaccgagcc
     3841 gcccttggca cccttgacgg cggcggtgac atccatcgtc accgtgccga cgcgcgggtt
     3901 cggcatcagg ccgcgcgggc ccagcacctt gccgagacga ccgaccagac ccatcatgtc
     3961 cggcgtggcg atgcagcgat cgaactcgat ggtgccgccg ttgacctttt cgaacaggtc
     4021 ctcggcgccg acgatatcgg caccggcagc cttggcctca tcggccttcg ggccacgggc
     4081 gaacaccgcg acgcgcaccg tgcggcccga gccgttcggc agattgcaga cgccgcgcac
     4141 gacctgatcg gcgtggcgcg gatcgacgcc cagattgagg gcgatctcga ccgtctcgtc
     4201 gaacttggcg gaagcgcgat ccttgatcag cttcacggcc tcgccgagcg gatagagctt
     4261 tgtgcggtcg atgccctcgc gggactttgc aacgcgcttg gcaaccatga cttaccccac
     4321 aacctcgacg cccatggaac gggcggaacc ggcgatcatc gcggacgcgg actcaacgct
     4381 gtcgcagttg aggtcggcca tcttcttctc ggcgatttcg gcgatctgct ttttggtgat
     4441 cttgccgacg aagccgcggc ccggcgtctt ggagccggcc ttgaggccga tcgccttcat
     4501 gatgaagaag ctcaccggcg gctgcttcat ctcgaaggtg aaggagcggt cctgataggc
     4561 ggtgatgatg acggggatcg gcgtcccctt ttccatctgc tgggtcttgg cgttgaaggc
     4621 cttgcagaat tccatgatgt tcagaccgcg ctgaccgagc gcggggccga tcggcggcga
     4681 cggattcgcc gcgccggccg gaacctgaag cttgatgaag ccaacgattt tcttggccat
     4741 agctctcttt ccgtcaaaat gccgatgtgc ccgccctccc cgcactcgcg gaaacggccg
     4801 gccaccggcg gttacaggat cgtggtccgg cttttgtctg acggagcgag aactccggcg
     4861 aaccgcctgc cacggggaca cctccggcgc gaggccggaa ggttgcagcg ggctatagtg
     4921 gaaagcggcc gggctgacaa gccgccgcgt tgcaacggcg caaggcgcct cctacatttc
     4981 aggcgggcgg aggcggcggc atgatcggcg aagcagcgtt actcaagatt ctcggcgcag
     5041 gtgtcgcgtt cggcgcgaaa gcctacaagg atattgccgg tctcaaggcc gaggatttca
     5101 gtgaagtcgc aaaatcgctt ggagaggcct tcatcggcgg caagcctgtc tccggcgaca
     5161 tgactgcctt catctccggc gtccagcgga gtatcgcgga cacgctcgac cagcgcacca
     5221 tggcggaggc cgggcgcaat ccggaggatg tgcgccgcga ccgggaaaac gcgctcgtgc
     5281 agttcaatga gacactgccg aagatggcgc tggatcgcgc cctgttcgcg cgcgcggagt
     5341 ttgacgctta cgccgtcgcg accgcgatga cggaccagct cgcgcgcctc aacccgctgt
     5401 tccgcccgcc cgcctctggt gcgccgcgct ccatcgccta tgcgctggcc tgcgagattt
     5461 tcttccgcac cttcgagctt gccgaacaga agaaggaatt ctgcgaaacc ctgctggcca
     5521 ccatccaccg cgaggtgttg aagcggctcg gccgcatcga ggacaatacg tcccgaacct
     5581 tggacgaact ggctgacatc aaggcacagc tcaaggccct cgccctcgcc aagggcgtcc
     5641 ccgaagaacc tctgcgcaag gtgctcgccc gcttcggcga ggagcatgtg ccgctgggcg
     5701 acattcccga cagactggcg cgcaaggcgg atgaactggt attcctgcgt caggagctgg
     5761 cacggctgcg caatgaccgg ccggaatttg ccgcccttcg cgcccgcgcg ctgaagctga
     5821 ttgacgatgg cgaattcgac gaggcccgcg ccgaactgcg caaggggcgc gaactggcaa
     5881 aagccatccg cgaggaacac gcccgcgagg aagccagctt tctgcgccac gaagcccgga
     5941 tcgaccgcct gaatctcgat tatgccgccg cgatcgccgc ctatcgtcag gccaccgcgc
     6001 ttgacccgga taatctgcct gcctgggggg aactcggcga cctctggatc atcctcggca
     6061 atctgacgga tgcgctcaaa gcctttgagg gggcggaagc tgcctgcatc cgatcgggcg
     6121 atgaacgcaa cctctcggtg tcgcacgaca ggatcggcga tgtccgcgtt gccaagggcg
     6181 atcttgccgg cgcgctcgag agctacaccg ccagcctcgc catccgccag acactcgccg
     6241 atgccgacag gaccaacagc ggatggcagc gcgatctctc ggtgtcgcac aagaagatcg
     6301 gcgatgtccg cgttgccaag ggcgatcttg ccggcgcgct cgagagctac atcgccagcc
     6361 tcgccatccg ccagacactc gccgatgccg acaggaccaa cagcggatgg cagcgcgatc
     6421 tctcggtgtc gcacaagaag atcggcgatg tccgcgttgc caagggcgat cttgccggcg
     6481 cgctcgagag ct
//
LOCUS       DAOMXN010000148         6436 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_6344_length_6436_cov_12.047485, whole genome shotgun sequence.
ACCESSION   DAOMXN010000148 DAOMXN010000000
VERSION     DAOMXN010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 6436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6436
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6344_length_6436_cov_12.047485"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..961
                     /locus_tag="PK812_10180"
     CDS             2..961
                     /locus_tag="PK812_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HRJ69966.1"
                     /translation="MIASIAAPGQPTRPAPFGHALTALAASRPDIVGLTADLSKYTDL
                     FVFAKAFPDRFYQVGMAEASLMSAAAGLAREGFTPFATTYAVFASRRAYDFICMAIAE
                     ESLNVKIVCALPGLTTGYGPSHQATEDIAIFRGLPNMTIIDPCDALDIEQIVPAIADH
                     RGPVYMRLLRGNVPVVLDEYGYTFELGKAKLVRDGRDVLVISTGLMTMRALEAAKVLR
                     ADRVDVGVLHVPTIKPLDIDTIVREAGKGGRLVVVAENHSIVGGLGEAVAGALLRAGV
                     TPVFRQIALPDEFLDAGALPTLHERYGISTEAVARRIRDWLGR"
     gene            1108..1389
                     /locus_tag="PK812_10185"
     CDS             1108..1389
                     /locus_tag="PK812_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRJ69967.1"
                     /translation="MSSTFDKVADIIAETADIDRAKVTPQAHVVDDLGIDSLAFLDIA
                     FAIDKAFGIKMPIEQWQQEVNEGKAAADNYFVMSNLCARIDELVAAKKA"
     gene            1409..1876
                     /locus_tag="PK812_10190"
     CDS             1409..1876
                     /locus_tag="PK812_10190"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010683688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HRJ69968.1"
                     /translation="MRLEYFQMIDRVTAFDREPPRLEATSVIPDQSPIFEGHFPGHPL
                     MPGVLLIETMAQTSGYLLLGLNGFARMPFLAGVTEAKLRRFVEPNTPIAMEASLVHEG
                     SGYAVTKARIRADGKAVCDAELMFKILPFPPGLGEQMRAHATGLGMPAVEAVP"
     gene            1873..3066
                     /locus_tag="PK812_10195"
     CDS             1873..3066
                     /locus_tag="PK812_10195"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HRJ69969.1"
                     /translation="MNARDVVITGVGMVSSLGDGLEAHAAALADPARVNIDAESFAPY
                     PVHPTPTLEYDRQIVKKLDQKQMEPWQRLGTYAAGLALEMAGLKGDKERLSNMLMVVA
                     AGGGERDYAVDGQILTQMPKEASPGVYLNERLMGDLRPTLFLAQLSNLLAGNISIVHG
                     VTGASRTFMGEEQAGVDALRIAVERIRARQGDSFLVGGSFNAERPDSLLIWAMGRMLR
                     KGAHVPVWSRKDDPGFMLGSGGAFLVLEAREVAAARGAKPLAVIADIEARRSRRAPGA
                     VAASLMDCLAAAKPALAGKKVAVLSGATGVAPITDEELSAIRAVLPDAPVRATGTRLG
                     HMIEAQFPVGIALAVQALQSGALFPAAANSPDEAPSTAAPEAVAVTCVGHQRGEGLAV
                     IVKAD"
     gene            3078..4352
                     /locus_tag="PK812_10200"
     CDS             3078..4352
                     /locus_tag="PK812_10200"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HRJ69970.1"
                     /translation="MKDTQGRPVIVVTGMGLVTSLGQGKADNWAALTAGKSGIKRIRR
                     FPVEGLKTTIAGTVDFVPAEPFCAAQLSENFARIAAQEAVSQAAIGRKGDFPGPLFVA
                     VPPVEIEWPQRLKVALAAAAPGDVTYADLLRVAENAEFRPWHDLFLFGTVADRLADSF
                     GTKGSPISLSTACSSGATAIQLGVEALRRGEADAALCIGTDGSVQAEALIRFSLLSAL
                     STQNDPPEGASKPFSKNRDGFVMGEGAAALVLETYASARARGATILGVIAGCGEKGDG
                     FHRTRSSPDGAPIIAALRAAIADAGVTPDDIDYVNAHGTSTPENDKMESLGCMTVLGE
                     RMKSVPISSNKSMIGHTLTAAGAVEAVISLMTLAHQKIPPTINYAVPDPAIPLDVVPN
                     VAREAKLRRVLSNSFGFGGQNTVLVFATEPTA"
     gene            4349..5098
                     /locus_tag="PK812_10205"
     CDS             4349..5098
                     /locus_tag="PK812_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRJ69971.1"
                     /translation="MSTNRRVLVTGGGKGVGAAIVRALSAAHYDVDFTYRSSAQQALE
                     LADQLTGGGVVVRALPVDLADKEAVESFCDLAEESGYYAFVHNAGQPYDVLAAMMAQD
                     KAEAAMQVNFWSMTRIVKAVVRPMLRARAGRIVGIGSVAALEGNQGNAAYAATKGAML
                     SYLRTLAVESARRGVTVNYLAPGFIDTDMMAPYAAYREQMEKQIPAGRFARPEDIAAL
                     VKFLLSADASYITGAVLPVDGGLTATIAFQR"
     gene            5133..6155
                     /locus_tag="PK812_10210"
     CDS             5133..6155
                     /locus_tag="PK812_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRJ69972.1"
                     /translation="MRALQLFGDRDLRLTEVDHPPHPGRAEVQVRVHAVGLNHIDVWG
                     FRGMAFAKRKMPLIVGAEAAGVVSAVGEDVTHVDVGDRVVMYGARTCGHCKACMQGRD
                     NLCENVGGIMGFHIDGFACDFVTMPQRLCVKIPDGVSFEEAACAPIAFGTVQHMLFDN
                     ARLEPGETILVQAGGSGIGTAAIKMAKAIGCTVITTVGDDDKGRKARDLGADHVINYR
                     EERFEGVVRKITGRKGVDVVFEHTGAETWNGSLLSMKKGGRLVTCGSTSGVSTTMNLM
                     QLFQQQYRIIGSFGCSIRNIEQSLKKMAEGITPVIDTVFPLADFAKGLDRLESRKVFG
                     KIIVRF"
     gene            6213..>6436
                     /locus_tag="PK812_10215"
     CDS             6213..>6436
                     /locus_tag="PK812_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69973.1"
                     /translation="MKQPNFFLRTLLKAGEMAMLALVSAVFALARALGPERASAVGGA
                     ITRTLGPLLPVHRVALANIAAAFPEKLEAER"
BASE COUNT         1065 a         2120 c         2140 g         1111 t
ORIGIN      
        1 gatgatcgcc tccatcgcgg cgcccggtca gccgaccagg ccggcgccgt tcggccatgc
       61 cttgaccgcg ctcgccgcca gccgccccga catcgtcggg ctgacggcgg acctgagcaa
      121 gtacacggac cttttcgtct tcgcgaaggc ctttcccgac cggttctacc aggtcgggat
      181 ggccgaggct tcgctgatga gcgccgcagc gggcctcgcg cgcgagggct ttactccctt
      241 cgccacgacg tacgccgtct tcgcctcgcg ccgcgcctat gatttcatct gcatggcgat
      301 cgcggaggaa agcctcaacg tcaagatcgt ctgcgcgctg ccgggattga ccacgggcta
      361 tggaccgagc caccaggcga cggaagatat cgcgatcttc cgcggcctgc cgaacatgac
      421 gatcatcgac ccctgcgacg cgctcgacat cgagcagatc gtgccggcca tcgcggacca
      481 taggggaccg gtctacatgc gcctgttgcg gggcaatgtg ccggtggtgc tggacgaata
      541 cggctacacg ttcgaactcg gcaaggcgaa gttggtgcgg gatggtcgcg acgtccttgt
      601 catctccacc gggttgatga ccatgcgcgc gctggaggcg gcaaaggtgc tgcgggccga
      661 ccgggtagac gtcggcgtgc tgcatgtgcc gaccatcaag ccgctcgaca tcgacaccat
      721 cgtccgggaa gcaggcaagg gcggacgcct ggtggtggtc gccgagaacc attccatcgt
      781 cggcggcctt ggcgaggcgg tggccggcgc gctgctccgg gctggcgtca cgcccgtctt
      841 ccgccagatc gcgctgccgg acgaattcct cgacgccggc gccctgccga cgcttcacga
      901 gcgctacggc atctcgaccg aggccgtggc gcggcggatc agggactggc tcggacgctg
      961 agggcggcat tgcgccgcag cagagattgg acaagcgccc cacgtttagt atagggcagg
     1021 gtccgaacag ggaccttgcc ctttgcattt cttgcaggcg gtgacggttg ctggctgaaa
     1081 tcccatgcaa atccgaagga gcgactgatg tcgtccacgt tcgacaaggt tgccgatatt
     1141 atcgccgaga cggccgacat cgatcgcgcc aaggtcaccc cgcaggccca tgtggtggac
     1201 gatctcggca tcgattcgct tgccttcctc gacatcgcct tcgccatcga caaggccttc
     1261 ggcatcaaga tgccgatcga gcaatggcag caggaagtga acgagggcaa ggcggcagcc
     1321 gacaactact tcgtcatgag caacctctgc gcccgcatcg acgaactcgt cgccgcgaaa
     1381 aaggcgtgac gagccgccgg ccctgcgcat gcggctcgaa tatttccaga tgatcgatcg
     1441 ggtaacggcg ttcgaccgcg aaccgccgcg ccttgaggcg acttcggtca tcccggacca
     1501 gagcccgatc ttcgaaggcc atttcccggg ccatccgctg atgccgggtg tcctgctgat
     1561 cgagacgatg gcgcagacct cgggctatct gctgctcggg ctcaacggat tcgcgcgcat
     1621 gccgttcctt gccggcgtca ccgaggccaa gctgcgtcgc ttcgtcgagc cgaacacgcc
     1681 gatcgcgatg gaggcgtcgc tggtgcatga aggctcgggc tacgccgtga cgaaggccag
     1741 gatccgggct gacggcaagg ccgtgtgcga tgccgaactc atgttcaaga tcctgccgtt
     1801 cccgcccggc ctcggcgagc agatgcgcgc ccatgcgacg ggcctcggca tgcccgcagt
     1861 ggaggcagtg ccatgaatgc ccgcgatgtc gtcataaccg gcgtcggcat ggtctccagc
     1921 ctcggcgacg gcctggaggc gcatgccgcc gccttggcgg accccgcgcg ggtcaacatc
     1981 gacgcagaaa gcttcgcgcc ctatccggtg cacccgacgc cgacgcttga gtacgaccgg
     2041 cagatcgtca agaagctcga ccagaaacag atggagccct ggcagcggct gggcacatat
     2101 gccgcgggcc tcgcgctcga gatggccggg ctgaagggcg acaaggaacg cctgtccaac
     2161 atgctgatgg tggtcgcggc cggtggcggc gagcgcgact atgcggtgga cgggcagatt
     2221 ctgacgcaga tgccgaagga ggcgagcccg ggcgtctacc tcaacgaacg cctgatgggc
     2281 gacctcaggc cgacgctgtt tctcgcccag ctctccaacc tgctcgccgg caacatttcc
     2341 atcgtccacg gcgtcaccgg cgcttcgcgc accttcatgg gcgaggaaca ggctggcgtt
     2401 gacgcgttgc gcatcgcggt cgagcgcatc cgggcgcgac agggcgacag tttcctcgtc
     2461 ggcgggtcat tcaacgcgga gcgtccggac tcgctgctga tctgggccat gggccggatg
     2521 ctgcgcaagg gcgcgcatgt tccggtgtgg agccgcaagg acgatccggg cttcatgctc
     2581 ggcagcggcg gcgcgttcct ggtgcttgag gcgcgcgaag tcgcggcggc gcgcggagca
     2641 aagccgctcg ccgtgatcgc cgacatcgag gcccggcgct caagacgcgc accgggcgcg
     2701 gttgccgcgt ccctcatgga ttgtctcgcc gcggcgaagc ctgcccttgc cggcaagaaa
     2761 gtcgcggtcc tttccggggc gactggcgtc gcgccgatca ccgatgaaga gctttccgcc
     2821 atccgcgccg tcctgccgga tgcgccggtc cgtgccacgg gcacgcgcct gggccacatg
     2881 atcgaggcgc agtttccggt cggcatcgcg ctcgccgtgc aggcgctcca gagcggagcc
     2941 ctgttcccgg cagccgcgaa ttcgccggat gaggcgccat ccaccgccgc tccggaggcc
     3001 gtcgccgtca cctgcgtcgg tcatcagcgc ggcgagggtc tggccgtgat cgtgaaggcg
     3061 gattgaggga ggcggcgatg aaagacactc agggccgtcc tgtcatcgtc gtcaccggca
     3121 tggggctggt gacgtcgctc ggccagggca aggccgacaa ctgggccgcg ctgaccgctg
     3181 gcaagtccgg catcaagcgc atccgtcgct tcccggtcga ggggctgaag acgaccatcg
     3241 ccggcaccgt ggattttgtg ccggccgagc cgttctgcgc cgcgcaactg tcggagaatt
     3301 tcgccaggat cgccgcgcag gaagccgtgt cgcaggcggc cattggccgc aagggcgact
     3361 ttcccggtcc gctgttcgtc gctgttccgc cggtcgagat tgagtggcca cagcgcctta
     3421 aagtggccct ggcagccgcc gcgcccggtg atgtcaccta tgccgacctg ctgcgcgtcg
     3481 ccgagaacgc cgagttccgc ccctggcacg atcttttcct gttcggcacg gtcgcggacc
     3541 ggctggccga cagttttggg accaaaggct cgccgatttc gctctcgacc gcctgttctt
     3601 ccggcgcgac ggcgatccag ctcggcgtcg aggcgctgcg caggggcgaa gcggacgcgg
     3661 ccctgtgcat cggcactgac ggttccgtgc aggccgaggc gctgatcagg ttttctttgc
     3721 tgtcggccct ctccacgcag aacgatcctc cggaaggcgc gtccaagccg ttctcgaaaa
     3781 accgcgacgg cttcgtcatg ggcgagggcg cagcggcgct ggttctggag acctacgcct
     3841 cggccagggc gcgcggtgcg accatcctgg gcgtcatcgc cggctgcggt gaaaaggggg
     3901 atgggttcca ccgcacgcgg tccagtccgg acggcgcccc catcattgcc gccctgcgcg
     3961 cggccatcgc cgatgccgga gtgacgcctg acgatatcga ctacgttaac gcgcatggca
     4021 cctcgacgcc ggaaaacgac aagatggaat cgctcggctg catgaccgtg ctcggcgaac
     4081 gcatgaaatc cgtgccgatc tcctccaaca agtcgatgat cggccacacg ctgaccgctg
     4141 ccggcgccgt ggaagcggtc atctcgctga tgacgctggc gcatcagaag atcccgccca
     4201 ccatcaatta cgccgttccc gatccggcaa tcccgcttga cgtggtgccg aacgttgcgc
     4261 gggaagccaa gcttcggcgg gtcctgtcca attccttcgg gttcggcggc cagaacaccg
     4321 tgctcgtctt tgccacggag ccgacggcat gagcaccaac aggcgcgttc tggtcacggg
     4381 gggcggtaag ggcgtcggcg cggcgatcgt ccgcgcgctg tccgctgccc attatgatgt
     4441 cgatttcacc tatcgctcgt ccgcccagca ggcactcgaa ctcgccgacc agctcaccgg
     4501 cggcggcgtc gtggtccggg cgctgccggt cgatctcgcc gacaaggaag ccgtcgagtc
     4561 tttctgcgac ctcgccgagg aaagtggcta ttacgcattc gttcacaatg ccggccagcc
     4621 ctatgatgtt cttgccgcga tgatggcgca ggacaaggcc gaggcggcga tgcaggtcaa
     4681 tttctggtcc atgacgcgca tcgtcaaggc cgtggttcgc ccgatgcttc gcgcgcgcgc
     4741 cggccggatc gtcggcatcg gatcggttgc cgcgctggaa ggcaaccagg gcaacgccgc
     4801 ctatgccgcc accaagggcg ccatgctttc ctatttgcgc acgctcgcgg tggaatcggc
     4861 caggcgcggc gttaccgtca actatctggc gcccggcttc atcgacacgg acatgatggc
     4921 gccctatgct gcctaccgcg agcagatgga gaagcagatt ccggctggtc gcttcgcccg
     4981 gccggaggac atcgcggcgc tcgtgaaatt cctgttgtct gcggatgcca gctacatcac
     5041 cggcgcggtg ctgcccgtgg atggcgggct gaccgcgacc atcgcctttc agcgctgaga
     5101 catcccagcg cctttttttg acggatagtg ccatgcgcgc ccttcaactc ttcggcgatc
     5161 gcgacctgcg cctgaccgag gtcgaccatc ccccgcatcc cggccgggcg gaagtccagg
     5221 tccgcgtgca tgccgtcggt ctcaaccaca tcgatgtctg gggctttcgc ggcatggcct
     5281 tcgccaaacg caagatgccg ctgatcgtcg gagcggaagc agccggcgtc gtctcggctg
     5341 tcggcgagga cgtaacccac gtggatgtcg gcgaccgcgt cgtcatgtat ggcgcgcgca
     5401 cctgcggtca ctgcaaggcc tgcatgcagg gccgcgacaa cctgtgcgag aatgtcggcg
     5461 gcatcatggg cttccacatc gacggcttcg catgcgactt cgtcaccatg ccgcagcggc
     5521 tgtgcgtgaa gattcccgac ggcgtttcat tcgaggaggc agcctgcgcg ccgatcgcct
     5581 tcggcacggt gcagcacatg ctgttcgaca acgctcggct ggagccgggc gagacgatcc
     5641 tggtgcaggc cggcgggtcc ggcatcggca cggcagccat caagatggcc aaggccatcg
     5701 gctgcaccgt gatcacaacg gtgggagacg acgacaaggg ccgcaaggcc agggaccttg
     5761 gtgccgacca cgtcatcaat taccgcgagg agcggttcga gggggtggtg cgcaagatca
     5821 ccgggcgcaa gggtgtcgat gtggtgttcg agcatactgg cgccgagacc tggaacggat
     5881 cgctcttgtc catgaagaag ggcggtcggc tggtgacctg cggctcgacg tccggcgtct
     5941 cgaccacgat gaacctgatg cagctgtttc agcagcagta tcgcatcatc ggctccttcg
     6001 gctgctcgat ccggaacatc gagcaaagcc tgaagaagat ggcggaaggc atcacgccgg
     6061 tgatcgacac ggtttttccg ctcgccgatt tcgccaaggg cctggatcgg cttgaaagcc
     6121 gtaaggtctt cggcaagatc atcgtgcggt tctgatgtcg gcttgacggc gcctccggcc
     6181 gccgccatag cgccggaaac gggcgggatc gcgtgaaaca gccaaacttt ttcctgcgaa
     6241 cgctgctgaa ggccggcgaa atggcgatgc tcgctctggt ttcggccgtg ttcgccctgg
     6301 cacgtgcgct cgggccggaa cgtgccagcg ccgtcggcgg cgcgatcacc cgcacgctcg
     6361 gaccgctgct tccggtgcac agggtcgcgc tcgcgaacat cgccgccgcg ttccctgaaa
     6421 agcttgaagc cgaacg
//
LOCUS       DAOMXN010000149         6363 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_6438_length_6363_cov_11.521560, whole genome shotgun sequence.
ACCESSION   DAOMXN010000149 DAOMXN010000000
VERSION     DAOMXN010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 6363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6363
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6438_length_6363_cov_11.521560"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="PK812_10220"
     CDS             <1..358
                     /locus_tag="PK812_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69974.1"
                     /translation="AAGSRGRAATTGRFVSATCASACVYVLAGGVKRVVPPESRIAVH
                     RMAGSVKSFDWSKWDYETRRVYAGDAEIASLTDYIRQMGVSTELVAMAESIPHEHARV
                     LSTREMARLKLAGSKF"
     gene            476..552
                     /locus_tag="PK812_10225"
     tRNA            476..552
                     /locus_tag="PK812_10225"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:510..512,aa:Asp,seq:gtc)
     gene            complement(612..1361)
                     /locus_tag="PK812_10230"
     CDS             complement(612..1361)
                     /locus_tag="PK812_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRJ69975.1"
                     /translation="MANTYDLKGKRAVVTGGAMGIGYAISERFAQSGARVALWDIDEA
                     AAKAAAAKLGAGCIAVPTDVTDYASVEAAARSTESTLGGIDILVCNAGIAGSNEPLDV
                     YPLDMWRRIMAINLDGVFHCLRAVTPGMKARKYGRIVNIASIAGKEGNPNACAYSASK
                     AGVIALTKSLGKELAGLDIAVNCITPAAARTAIFDQMKPEFIQFMLSKIPRGRFVQVE
                     EIAALASWLASAENSFTTGGVFDISGGRATY"
     gene            1532..1897
                     /locus_tag="PK812_10235"
     CDS             1532..1897
                     /locus_tag="PK812_10235"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011975081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HRJ69976.1"
                     /translation="MQLLLGDYLPLVVFIGVSCVIGLALLIAPFVVAYKAPDAEKLSA
                     YECGFNAFDDARMKFDVRFYLVAILFIIFDLEVAFLFPWAVAAREIGWFGFWSMMTFL
                     AVLTIGFIYEWKKGALEWD"
     gene            1930..2514
                     /locus_tag="PK812_10240"
     CDS             1930..2514
                     /locus_tag="PK812_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HRJ69977.1"
                     /translation="MSVTALDRGNPLVAPAPKGLVGPDGRPVGSADPYFVDINAELAD
                     KGFIVTATDDLIAWARTGSLMWMTFGLACCAVEMMQLSMPRYDVERFGFAPRASPRQS
                     DVMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVP
                     VDIYVPGCPPTAEALLYGVLLLQKKIRRTATIER"
     gene            2640..3245
                     /locus_tag="PK812_10245"
     CDS             2640..3245
                     /locus_tag="PK812_10245"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HRJ69978.1"
                     /translation="MTEALSSLGEHIQAALPGAVTGWSVAFGELSLSANAADIVNVAS
                     FLKTDARCQFLSFIDIAGADYPGRDKRFDVVYHFLSPKLVQRIRVKVQADEETPVPSL
                     TGLFPGADWFEREAYDFYGILFTGHHDMRRILTDYGFEGHPLRKDFPTTGYVEVRYDD
                     QEKRVVYDKVRLNQEMRQFDFLSPWEGVEYQLPGDEKAKAQ"
     gene            3254..4447
                     /locus_tag="PK812_10250"
     CDS             3254..4447
                     /locus_tag="PK812_10250"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HRJ69979.1"
                     /translation="MTEQPHLRNFTINFGPQHPAAHGVLRLVLELDGEIVERVDPHIG
                     LLHRGTEKLIEQKTYLQAVPYFDRLDYCAPMNQEHAFCLAVEKLLGIGVPRRGQLIRV
                     LYSEIGRLLSHLLNVTTQAMDVGALTPPLWGFEEREKLMIFYERASGSRMHAAYFRPG
                     GVHQDIPAELINDIEAFCDPFLKVCDDLEGLLTDNRIFKQRNVDIGVVSLEDCWKWGF
                     SGVMVRGSGAAWDLRKAEPYECYSELDFDIPIGKNGDCYDRYCIRMEEMRQSIRIMKQ
                     CCALLRKPDGQGPVSTTDGKVAPPKRGEMKRSMEALIHHFKLYTEGFKVPAGEVYAAV
                     EAPKGEFGVYLVSDGTNKPYRCKIRAPGFAHLQSMDFMCRGHMLADVSAILGSLDIVF
                     GEVDR"
     gene            4458..5375
                     /locus_tag="PK812_10255"
     CDS             4458..5375
                     /locus_tag="PK812_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69980.1"
                     /translation="MTADHSRLKAELRAIPKAELKRFWADLGYILLPAAPEWRDRPET
                     DLQRERLLSAVTEALAEDIDACPDLAPHPGPLPASGQREQTIAAIVRDFYDLIPTSPV
                     NAKKGGNGFNGCLQVYAVARLLNPKVIIESGVFRGQTTWILRQACPKATIFCFDVDFS
                     NLVYKDREAIYTQSDWSGFDLAGLPTDETLAFFDDHIDQGRRVLEAQVRGLSHLLFDD
                     NASASRIHTVGGPPIPSLDMLMDPALDGQTLAWTYRGKRYGCAIDAAQAQQVRKAVAT
                     MRNFADMHRVTGYTPARITYVSLCREAKA"
     gene            5397..5657
                     /locus_tag="PK812_10260"
     CDS             5397..5657
                     /locus_tag="PK812_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69981.1"
                     /translation="MTALIASGLLTGEPQAHNAQATPPNANTVTLEMLARDGYEVKAI
                     QSATTRGFGFVVMMQRGAELRTCLMRINRGPDGRPARESVCF"
     gene            5671..>6363
                     /gene="nuoE"
                     /locus_tag="PK812_10265"
     CDS             5671..>6363
                     /gene="nuoE"
                     /locus_tag="PK812_10265"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HRJ69982.1"
                     /translation="MTVRRLAPAEVQPASFAFSNDNKAFVAQQVAKYPPGRQASAVIP
                     LLWQAQKQAGGWLPQKAIEHVADTLGMPYIRVLEVATFYTMFNLQPVGTHFIQLCGTT
                     PCALRGAEKLKDVLRQRVGDQNHVTADGKFSWLEVECLGACCNAPMVQINDDFYEDLT
                     PDSLNTLLDDLAAGRKVRIGPQNGRRSSEPEGAVKTLTDATLYDGSAVGSWRTAFDER
                     FARAQAAKTATAS"
BASE COUNT         1117 a         2042 c         2028 g         1176 t
ORIGIN      
        1 ggccgccggg agcagggggc gggcagcgac gaccggtcgg ttcgtgtcgg caacctgcgc
       61 ctcggcctgc gtctatgtcc tggccggcgg cgtgaaacgc gtggtgccgc cggaaagtcg
      121 cattgccgtg catcgcatgg cggggagcgt caagagcttc gactggtcca aatgggacta
      181 tgagacccga cgcgtctacg cgggcgatgc cgaaatcgca tccctcaccg actatatccg
      241 ccagatgggc gtcagcaccg aactggtggc gatggccgag tcgattcccc atgaacatgc
      301 gcgcgtcctg tccaccaggg aaatggcgcg cctgaagctg gccggcagca agttctgacg
      361 gctccaaacc tgactgggat cgctgcatca atcgctgcga tctctgcttg acttgccggc
      421 gggcgcaccg tatcccgccg tcctctgccg gagcggttcg cttcggacga aatgcgcggg
      481 cgtagctcag tcggttagag tgccggcctg tcacgccgga ggtcgcgggt tcgagccccg
      541 tcgcccgcgc cacttctccc ctgacgccga tgaacgcatg gccccttttg gggcagggtc
      601 cttttgtgga ttcaataggt cgcccgtccg ccggaaatgt caaagacacc gccggttgtg
      661 aacgaattct ccgcgctcgc cagccagctc gccagggcgg cgatctcctc gacctgcacg
      721 aaccggccgc gcgggatttt cgacagcatg aactggatga attcgggctt catctgatcg
      781 aagatggcgg tgcgcgccgc cgccggcgtg atgcaattca cggcgatgtc gagcccggcc
      841 agttccttgc ccagcgactt ggtcagcgcg atcacgccgg ccttggaggc agaataggcg
      901 caggcattgg ggttgccttc cttgccggcg atggacgcga tgttgacgat gcggccgtac
      961 ttgcgcgcct tcatgcccgg cgtgaccgcc cgcaagcagt gaaacacgcc atccagattg
     1021 atggccatga tcctgcgcca catgtcgagc gggtagacat ccagcggctc gttcgatccg
     1081 gcgatcccgg cgttgcagac caggatatcg atgccgccca gcgttgattc ggtggacctg
     1141 gccgcagctt ccaccgaggc ataatccgtg acgtcggtcg ggaccgcgat gcagccggca
     1201 ccgagtttgg ctgccgcggc ttttgccgcc gcttcgtcga tatcccacaa ggcaacccgt
     1261 gcgccggact gcgcaaagcg ctccgatatc gcgtaaccga tgcccattgc gccgcccgtg
     1321 acgactgcgc gcttgccctt gagatcatag gtgttggcca tgtccgctcc tgttgtcaga
     1381 caagttgaaa cccaggaact gcgacttgtc gagggggctg cggcgtcctg caacttttgc
     1441 ttcacttgcc acacccggcg gtgtgggcta atccctctcg cgccggcgca gcgcggcccg
     1501 aattcgcgcg cccggtccgg aaaggcatcc tatgcagctc ctcctcggcg actatctccc
     1561 ccttgtcgtg ttcatcggcg tttcctgcgt gatcggcctg gcgctgctga ttgccccctt
     1621 cgtggtggcc tacaaggctc cggatgcgga aaagctgtcg gcttacgagt gtggattcaa
     1681 cgcgttcgac gatgcgcgca tgaagttcga cgtgcgtttt tatctcgtgg cgattctctt
     1741 catcatcttt gatctcgaag tggccttcct gttcccctgg gcggtcgcgg cgcgcgagat
     1801 cggctggttc ggattctggt cgatgatgac gtttctcgcc gtgctgacca tcggcttcat
     1861 ctacgaatgg aaaaaaggcg cactggagtg ggattgatcc cgctgcgcgc gcatttttga
     1921 ggactgcaca tgtctgtgac agcactggat cgcggaaatc cgctggtcgc cccggcgccc
     1981 aagggcctcg tgggtcccga cggcaggccg gttggatcgg cggatcccta tttcgtcgac
     2041 atcaacgccg aactcgccga caaggggttc atagtaaccg cgacggacga cctgatcgcc
     2101 tgggcgcgca ccggctcgct gatgtggatg acattcggcc tcgcctgctg cgcggtcgag
     2161 atgatgcagc tctcgatgcc gcgctacgat gtggagcggt tcggcttcgc cccgcgcgcc
     2221 tcaccccgcc agtccgacgt gatgatcgtc gccggtacgc tgaccaacaa gatggctccg
     2281 gcgctgcgca aggtctacga ccagatgccg gagccgcgct acgtcatctc catgggctcc
     2341 tgcgccaacg gcggcggtta ctatcactac agctattccg tggtgcgtgg ctgtgaccgc
     2401 atcgtgccgg tcgacatcta cgtgccgggc tgcccgccga cggcggaagc gttgctctac
     2461 ggcgttctgc tcctgcagaa gaagatccgg cggacggcga cgatcgagag ataggcgcca
     2521 agaggcgcgg atacgcaggc tcggcccgtg tatctacgac ttggacggac taggtaagtc
     2581 gtcgatggcc ggaacacgct tggccatgac gacgcggaga acgggaaacg acgaagacca
     2641 tgaccgaagc cctttcctcc cttggcgaac atatccaggc ggctttgccg ggcgcggtga
     2701 ccggctggtc cgtcgccttc ggggagttga gcctttccgc caacgcggct gacatcgtca
     2761 atgtcgcaag cttcctgaag acggacgcgc gctgccagtt cctgagcttc atcgacatcg
     2821 cgggcgcgga ctaccccggt cgcgacaagc ggttcgacgt ggtctaccac ttcctgtcgc
     2881 cgaagctggt gcagcgcatc cgcgtcaagg tgcaggcgga tgaggaaacg ccggttccct
     2941 cgctgaccgg gctgtttccg ggtgcggact ggttcgagcg cgaggcctac gatttctacg
     3001 gcatcctgtt caccggccac cacgacatgc gccgcatcct cacggactat ggcttcgagg
     3061 ggcatccgct gcgcaaggac ttcccgacca cgggctatgt cgaggtgcgc tacgacgacc
     3121 aggaaaagcg cgtcgtctac gacaaggtgc gcctgaacca ggaaatgcgg cagttcgact
     3181 tcctctcgcc gtgggaaggg gtcgagtatc aattgccggg cgatgagaaa gccaaggcgc
     3241 agtgagctga tccatgaccg aacaaccgca tctgcgcaat ttcaccatca acttcggccc
     3301 gcagcatccg gccgcgcacg gcgtgctgcg cctggtgctg gagcttgacg gcgagatcgt
     3361 cgagcgcgtc gatccgcata tcggcctcct gcatcgcggc acggaaaagc tgatcgagca
     3421 gaagacctat ctgcaggccg tgccctactt cgatcggctg gactattgcg cgccgatgaa
     3481 tcaggagcac gccttctgcc tcgcggtgga gaagctgctc ggcataggcg tgccgcgccg
     3541 tggccagctc attcgcgtac tctattccga gatcggacgc ctgctgtctc atctgctcaa
     3601 cgtcaccacg caggcgatgg acgtcggcgc gctaaccccg ccgctgtggg gcttcgagga
     3661 acgcgagaag ctgatgatct tctacgagcg ggcctccggt tcgcgcatgc atgcggccta
     3721 tttccggccg ggcggcgtgc atcaggacat cccggccgaa ctcatcaacg acatcgaggc
     3781 gttctgcgat ccgttcctca aggtctgcga tgacctggaa ggcctgctca ccgacaaccg
     3841 catcttcaag cagcgcaacg tcgacatcgg cgtcgtctcg ctggaggatt gctggaagtg
     3901 gggcttctcc ggcgtgatgg tgcgcggctc cggcgcggcc tgggacctgc gcaaggccga
     3961 accctacgag tgctattcgg aactcgattt cgacattccg atcggcaaga acggcgactg
     4021 ctacgaccgc tactgcatcc gcatggaaga gatgcgccag tccatccgca tcatgaagca
     4081 gtgctgcgcg ctgctcagga agccggacgg gcaggggccg gtctccacca cggacggcaa
     4141 ggtcgcgccc ccgaagcgcg gcgaaatgaa acgctcgatg gaagcgctga tccaccactt
     4201 caagctctac accgagggct tcaaggtgcc ggccggcgag gtttacgccg ccgtcgaggc
     4261 gccgaagggc gaattcggcg tctatctcgt ctcggacggc accaacaagc cgtatcgctg
     4321 caagatccgc gcaccgggct tcgcgcatct ccaatccatg gatttcatgt gccgcggcca
     4381 catgctggcg gacgtgagcg cgatcctggg ctcgctggat atcgtgttcg gggaagtgga
     4441 caggtaaggc ggtcgcgatg accgccgatc attcccgcct caaggccgaa ctgcgcgcga
     4501 tccccaaggc cgaactcaag cggttctggg ccgatctagg ctatatcctg ttgccggcgg
     4561 cgccggaatg gcgagaccgt ccggagacgg acctccagcg tgaacggctg ctgtctgcgg
     4621 tgaccgaggc gctggcggag gatatcgatg cgtgcccgga ccttgcccct caccccggcc
     4681 ctctccccgc gagcgggcag agggagcaga cgatcgccgc catcgtgcgc gatttctacg
     4741 acctgatccc gacatcgccc gtcaacgcca agaagggcgg caatggcttc aacggctgcc
     4801 tgcaagtcta tgccgtcgca cggcttctga acccgaaggt catcatcgaa tcgggtgtct
     4861 ttcgcggcca aaccacctgg attttgcggc aggcttgtcc gaaagcgacg atcttctgct
     4921 tcgacgtgga tttttccaat ctcgtctaca aggatcgcga ggcgatctac acacaatccg
     4981 attggtccgg gttcgacctt gccggcctgc cgacggacga gacgctggct ttcttcgacg
     5041 atcacatcga ccaggggcgc cgtgtgctgg aggcgcaggt gcgcggattg tcgcacctgc
     5101 tgttcgacga caacgcgtcg gccagccgga ttcacacggt cggtgggcca ccgattcctt
     5161 cgctcgacat gctgatggac ccggcgctgg acgggcagac actggcctgg acctatcgcg
     5221 gcaagcgcta cggctgcgcc atcgacgcgg cgcaggcgca acaggtgcga aaagctgtcg
     5281 cgaccatgcg gaatttcgca gacatgcatc gcgtcacggg ctatacgcct gcgcggatca
     5341 cctacgtctc gctttgtcgg gaagcgaagg catgagggcg tgccatgatg actgcgatga
     5401 cggcgctcat cgcgtccgga ctgttgacag gggagccgca ggcgcacaat gcgcaggcca
     5461 ccccaccaaa tgccaacacc gtgacgctgg aaatgctcgc gcgcgacggt tacgaggtga
     5521 aggcgatcca gagcgcgacg acgcgcggct tcgggtttgt ggtcatgatg cagcgagggg
     5581 cagagctgcg cacctgcctc atgcgcatca accgtggtcc ggacggccgt cccgcccgcg
     5641 agtccgtttg tttctgatga ggctgatgcc atgactgtcc gccgtctcgc gcccgctgaa
     5701 gtccagcccg cgagttttgc gttctcgaac gacaacaagg ccttcgtcgc gcagcaggtc
     5761 gcgaagtatc cgccgggccg ccaggcctcg gccgtgatcc cgctgctctg gcaggcgcag
     5821 aagcaggccg gcggctggct cccgcagaag gcgatcgagc acgtcgcgga tacgctgggc
     5881 atgccctaca tccgcgtgct ggaagtcgcg accttctaca cgatgttcaa tctccagccg
     5941 gtcgggacgc atttcatcca gctctgcggc accacgccct gcgcgctgcg cggcgcggaa
     6001 aagctgaagg acgtgctgcg tcagcgcgtc ggcgaccaga atcacgtcac cgccgacggc
     6061 aagttctcct ggctggaagt ggagtgccta ggtgcatgct gcaacgcgcc gatggtgcag
     6121 atcaacgacg acttctatga ggacctgacg ccggacagcc tcaatacgct gctcgatgat
     6181 ctcgccgccg gccgcaaggt caggatcggg ccgcagaacg gccgccgctc ctcggagccg
     6241 gaaggcgccg tcaagacgct gaccgatgcg accctgtacg acggctcggc cgtcggttcc
     6301 tggcgcacgg cgttcgacga acggtttgcc agggcgcagg ccgccaagac cgctacagct
     6361 tcg
//
LOCUS       DAOMXN010000150         6359 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_6445_length_6359_cov_12.717957, whole genome shotgun sequence.
ACCESSION   DAOMXN010000150 DAOMXN010000000
VERSION     DAOMXN010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 6359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6359
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6445_length_6359_cov_12.717957"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..386
                     /locus_tag="PK812_10270"
     CDS             54..386
                     /locus_tag="PK812_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69983.1"
                     /translation="MERVQALAGTDWRIALARTPHFAEYILYGVFADKILGLEKAGLF
                     ATDETLCHARWSDGFSGTDEVDSFIHDLGPGHVAFALQSTIDIPIAARRAICERMARL
                     AAERDAQA"
     gene            complement(408..1784)
                     /locus_tag="PK812_10275"
     CDS             complement(408..1784)
                     /locus_tag="PK812_10275"
                     /inference="COORDINATES: protein motif:HMM:NF021589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRJ69984.1"
                     /translation="MRTLEDSALRRRAAASRGGLRGRLARVLFGALISLGAMAGTTPL
                     RAQYLDPPPTVGDDVFDARARKPRSPPDPYKKPGQDYRGILLGDVIFFPKVFIGATYD
                     DNLTWAARNQTDGFGVKINPGFVALRDAGIHKTLVYADVDARLFPGLTKGNAVNLRAG
                     LEQMWEVTRDFVIKAKLQYDHKESYADSSALDPLTGAITSLTTPLKSNQFLAGLTAKY
                     SFGNAFLGASLETVKTTYQALHSASGRVSQSYRDSLVNTVTLRAGYWLTPAFYAFAET
                     TGNIRDYSDDDYRSRGYRVTAGIGTDRIGLVRGEIYSGVQKQFYDQPAIKDTFSPVFG
                     GRLFWYPTRDLTFRASLDQSFTDSSTPSRANPNGNPARKTSAELNMRYQIDKSWAFSW
                     RAGYDYHAFIGVPRRDHSWRTGVTLSYDIFRNAALTLDYDFSRTISNTTGIGNVRNAI
                     MFGMNYRF"
     gene            complement(1834..2550)
                     /locus_tag="PK812_10280"
     CDS             complement(1834..2550)
                     /locus_tag="PK812_10280"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HRJ69985.1"
                     /translation="MTRTLPRHAALLAVLPLGLAVSACESTLTLGSSEPAHTNSTAAK
                     PIGPVAAAPQALPEAAGKAMPLNSALLPKEAKTTLPEDVVKPAQRLQGGDKVRIVVYG
                     EDKLTGDYEIDQSGAIAMPLAGVVKAEGLTKDELEKAIARRLSQARILKNPIVTVSVA
                     SFRPFYVLGEVERPGGYAYQNGLNAMSAVALAGGYTYRASKSKVMIQRAGEKGFTQYP
                     LSPEVQIFPGDLISVPERYF"
     gene            complement(2580..3380)
                     /locus_tag="PK812_10285"
     CDS             complement(2580..3380)
                     /locus_tag="PK812_10285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HRJ69986.1"
                     /translation="MSSVAFVDTGQPAAGPEVAVNLSNMHSTLEAIGERARSGEGFTL
                     FTINLDHVVKLNENDAFREAYGRANFVTADGWPVVWLIDQEGSGLERTTGADLLEPMC
                     RQASEMGYSVYFVGPGPESQRKGLGILTTRYPGLKVAGAEAPQLPSTLDDALVDAMAG
                     RITASGARICVLSLGAPKQELLADALHQRCPNVGFLCIGAALDFISGHASRAPDWVKR
                     AKLEWCWRLVHDPMRLTLRYANCAFALVKLALPGIFRRGPRLKLADPK"
     gene            complement(3505..4485)
                     /locus_tag="PK812_10290"
     CDS             complement(3505..4485)
                     /locus_tag="PK812_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRJ69987.1"
                     /translation="MSAEHAPVSLTIILCTFHREALLRQALQSLLGQQVPRGCQVEIV
                     VVDNSDAGTARSVVESLKSASPFPLRWIEAHPANISVARNAGVAAAATPFIGFLDDDQ
                     RVDPGWLEAVARAARNPDFDAHFGRNEARFETPERTTPAILQLFTRVLDAPAGTELFA
                     MGPRKTPGITLATANSLFRRAAMLVGPGPFDPAFGAGGGEDYDLICRMQRRGSRFAWM
                     PDAVASELVPASRCAPAYMRRRIYAGGQAYAAAVSNASDHPARMRWLMRLKAIVQAAL
                     MLPLFAVRAAQGRAALLDHGFAFAGVLGKIAFGAIHPLYRRVDAEERAGR"
     gene            complement(4482..5867)
                     /locus_tag="PK812_10295"
     CDS             complement(4482..5867)
                     /locus_tag="PK812_10295"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRJ69988.1"
                     /translation="MTPTAWSTSGHISYAPEDGVENREISGPRLWLEHFLLAGFLFIT
                     FVGTQPFLGASIESRSEGSPLDRLIVLALFAVALVVLFGRWRDALRCVGANLPLFLLV
                     GFCVASILWSNFPGITLRRGMLLFFLTTIALAVAVSTDDLRRLHTVLFATLSVLVLIC
                     LLLALGVPSVGMSEIGAKGFYTQKNVAGIVAMITVVLAVTWMMGSREGWQKALALAAL
                     IPVLVFLVLTKSKTSINLTALAVLVICAFALAERYGPAMIIAGGLFGALLLIGSALWL
                     AILDFDIDAAMRTIIGDTSFSGRDELWDFVRRDIARSYWLGHGYGAYWDVGLANDPLV
                     RAEVGSWLEAVPIGVVNQAHDGYLDLWVQMGLPAVVIATLVTLKATFASAHYALASHE
                     PVEDRALVGAMAAVLVIYLLHNFTEATLFVRGSIFHSSATLAIFLASHAWQVDRDLRL
                     SDDDPEPDEDA"
     gene            5995..>6359
                     /locus_tag="PK812_10300"
     CDS             5995..>6359
                     /locus_tag="PK812_10300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HRJ69989.1"
                     /translation="MDTVSIVIPTYNRNRSLERALASALAQQQTCGSVSLEIIVVDNS
                     PDGNAAAIVAAHAGGPVAVRSLHEPKTGVANARNAGVAAAQGRWVAFLDDDEEASPDW
                     IARLTETARATGADAVFGPV"
BASE COUNT         1143 a         1963 c         2241 g         1012 t
ORIGIN      
        1 gtcgagaaag cgttcgcctt cgcgacagac gctgagttgg tacgagcggc gcggtggagc
       61 gggtgcaggc gctggcgggc acggactggc gcatcgcgct ggcgcgaacg ccgcatttcg
      121 ccgaatacat cctctacggt gtcttcgccg acaagatcct cgggctggag aaggccggcc
      181 tgttcgcgac cgacgagacc ctgtgccacg cccgctggag cgacgggttt tccggcacgg
      241 acgaagtcga ttccttcatc cacgacctcg gccccgggca tgtcgccttc gccctgcaat
      301 cgacgatcga cattccgatc gcggcgcggc gggcgatctg cgagcggatg gcgaggctgg
      361 cggcggaacg ggacgcgcag gcctgaggcc cttcggaaag tccgcgttca gaagcggtag
      421 ttcatgccga acatgatggc attgcgcaca ttgccgatgc cggttgtgtt cgagatggtg
      481 cgcgagaagt catagtccag ggtcagcgcg gcgttgcgga agatgtcgta ggacagagtg
      541 acgccggtgc gccagctgtg gtcgcggcgc ggcacgccga tgaaggcgtg gtagtcgtat
      601 ccggcgcgcc aggagaaggc ccaggacttg tcgatctggt agcgcatgtt caattcggcc
      661 gaggtcttgc gggccgggtt gccgttgggg ttcgcgcgcg aaggcgtcga ggaatcggtg
      721 aagctctggt cgagcgaggc gcggaaggtc agatcgcgcg tcgggtacca gaacaggcgc
      781 ccgccgaaga ccgggctgaa cgtgtccttg atggccggct ggtcgtagaa ctgcttctgc
      841 acgccggagt agatttcgcc gcgcaccagg ccgatgcggt ccgtgccgat gccggcggtc
      901 acccggtatc cccgcgagcg gtagtcgtcg tcggagtagt cccggatgtt gcccgtggtc
      961 tctgcgaagg catagaaggc gggcgtcagc cagtagccgg cgcgcaaggt caccgtgttg
     1021 accaggctgt cccggtagct ttgcgaaacc cgccccgaag ccgagtgcag ggcctgatag
     1081 gtggtcttca ccgtttcgag cgaggccccg aggaaggcgt tgccgaagga gtatttcgcg
     1141 gtgaggccgg ccaggaactg gttggatttc agcggcgttg tcagcgatgt gatggcgccg
     1201 gtcaggggat cgagcgcgct gctgtccgca tagctttcct tgtggtcgta ctgcagcttg
     1261 gccttgatga cgaagtcgcg tgtgacctcc cacatctgtt ccaggcctgc acgcaggttg
     1321 acggcattgc ccttggtcag cccgggaaac aggcgcgcgt ccacatcggc atagaccagc
     1381 gtcttgtgga tgccggcatc ccgcagcgcc acgaagccgg gattgatctt cacgccgaaa
     1441 ccgtcggtct ggttacgggc ggcccaggtc aggttgtcgt cgtaggtcgc gcctatgaac
     1501 accttgggaa agaaaatcac gtcaccgagc aggatgccgc gatagtcctg gccgggcttc
     1561 ttgtagggat cgggcggcga gcgcggcttg cgggcgcggg catcgaagac atcgtcgccg
     1621 accgtcggcg ggggatcgag atactgggcg cgcagcgggg tcgtgccggc catcgcgccc
     1681 agagagatga gtgcgccgaa aaggactcgc gccagccggc cgcgcagacc gccgcgggat
     1741 gcggcggcgc ggcggcgaag ggcggaatcc tcaagggttc tcacgttctt gcgtccgtaa
     1801 gcgatcaggc ttcagcggcc ttatcgaagg ggtctagaag tagcgttcgg gcacggagat
     1861 caggtcgccg gggaagatct ggacttccgg cgagagcggg tattgcgtga agcccttctc
     1921 gcccgcgcgc tggatcatca ccttcgactt ggaggcgcga taggtgtagc cgccggccag
     1981 ggcgaccgcg ctcatggcgt tgaggccgtt ctggtaggca tagccgcccg gtcgctcgac
     2041 ctcgccaagc acgtagaacg ggcggaagga cgccacgctg accgtgacga tgggattttt
     2101 caggatgcgc gcctggctga gacggcgggc aatcgccttc tccagttcgt ccttggtcag
     2161 gccttctgcc ttgacgacgc ccgccagggg catggcgatg gcgccggact gatcgatttc
     2221 gtagtcgccg gtcagcttgt cctcgccgta gacgacgatg cgcaccttgt cgccgccctg
     2281 caggcgttgc gccggcttga caacgtcctc gggaagcgtg gtcttcgcct ccttcggcaa
     2341 cagggcggaa ttgagcggca ttgccttgcc ggccgcttcg ggaagtgcct gcggcgccgc
     2401 agccaccgga ccaatgggct tggccgcggt gctgttggta tgggcgggtt cgctcgaccc
     2461 gagcgtcagt gtgctctcgc aggccgaaac ggccaggccg aggggcagca cggcgagcaa
     2521 ggcggcgtgg cggggaaggg tgcgtgtcat cgaacggaac tctcggacca gaggtatcgt
     2581 catttcggat cggccagttt gaggcgtggt cccctccgga agatgccggg cagcgccagc
     2641 ttcaccagcg cgaaggcgca gttggcgtag cgcagcgtca ggcgcatcgg atcgtgcaca
     2701 aggcgccagc accattcgag cttggcacgc ttcacccagt ccggggcgcg ggaggcgtga
     2761 cccgagatga aatcgagcgc tgcgccgatg cagagaaaac cgacgttcgg gcagcgctga
     2821 tgcagggcgt cggcaagcag ttcctgcttc ggcgcgccaa gcgacagcac gcagatgcgg
     2881 gcacccgagg ccgtgatcct cccggccatg gcgtcgacca aggcgtcatc gagcgtgctt
     2941 gggagttgcg gggcctcggc gccggcgacc ttgaggccgg ggtagcgcgt ggtgaggatg
     3001 cccagtccct tgcgctggga ttccgggccg ggaccgacga aatagacgga ataccccatc
     3061 tccgaagcct gccggcacat gggttccaga aggtcggcgc cggtggtgcg ctccagcccg
     3121 ctgccttcct ggtcgatcag ccagacaacg ggccagccgt cggcggtgac gaagttcgcg
     3181 cggccatagg cctcgcggaa cgcatcgttc tcgttgagct tgaccacgtg gtcgaggttg
     3241 atggtgaaga gcgtgaagcc ttcgcctgac cttgcccgct cgccgatcgc ttccagcgtg
     3301 ctgtgcatgt tcgacagatt gacggcgact tcaggaccgg cagcaggctg gccggtgtcg
     3361 acgaacgcga ccgaactcat acgcgtactc cagaccaaac cgcgcaatga tactggcgtg
     3421 acgctaggtc atttacgtga aagcaaggtt aaccggagcg cctgaaaccg cgcgaagtga
     3481 ttcaaaagcg cacagactcc ggtgtcagcg ccctgcgcgc tcctcggcat ccacgcgtct
     3541 gtagagggga tggatcgcgc cgaaagcgat cttgccaagc acgccggcga aggcaaagcc
     3601 gtgatcgagc agggcggcgc gaccctgggc ggcgcggacg gcgaacagcg gcagcatcag
     3661 cgcggcctgg acaatcgcct tcaggcgcat gagccagcgc atgcgtgccg gatggtcgga
     3721 ggcattgctt accgcagccg cataggcctg ccctcccgcg tagatgcggc gtcgcatgta
     3781 ggccggggcg caccgcgagg ccggcaccag ttcgctggct acggcgtcgg gcatccaggc
     3841 gaagcggctg ccgcgccgct gcatgcggca gatcaggtcg tagtcctcgc cgcctccggc
     3901 gccgaaggcc ggatcgaacg gaccgggacc gaccagcatg gcggcgcggc ggaacagtga
     3961 attggcggtc gcgagcgtga tccccggcgt cttgcgaggg cccatggcga aaagttccgt
     4021 gcccgccggc gcatcgagca ctcgggtgaa gagctgcagg attgccggcg tggtgcgctc
     4081 cggcgtctcg aaacgggctt cgtttcggcc gaaatgggcg tcgaagtccg gattccgcgc
     4141 ggctcgcgcg acagcctcaa gccaaccggg atcgaccctc tgatcgtcgt cgagaaagcc
     4201 gatgaagggc gtcgcagccg ctgcgacgcc ggcattgcgc gcgaccgaga tgttggccgg
     4261 atgggcttcg atccagcgca aaggaaacgg gctggcggac ttgaggctct cgacgacgga
     4321 ccgcgccgtc cccgcgtcgg aattgtcgac gacgacgatt tcaacctgac agccgcgcgg
     4381 aacctgttga ccgagcaggc tctggagcgc ctggcggagc agcgcctcgc ggtgaaacgt
     4441 gcagaggatg atggtcaggc tgactggggc atgctccgcg ctcatgcatc ctcgtcaggt
     4501 tctgggtcgt catcgctcag ccgaaggtcg cgatccacct gccaagcatg ggaggcgaga
     4561 aagatggcga gcgttgccga gctgtggaag atcgatccgc gcacgaacag cgttgcctcg
     4621 gtgaaattgt gcagcaggta gatcaccagg acggctgcca tcgccccgac cagggcgcga
     4681 tcctcgaccg gttcatggct ggccagcgca taatgggcgc tggcgaaggt ggccttgagc
     4741 gtgaccagcg tggcaatcac cacggcgggc aggcccattt gcacccacag gtcgagatag
     4801 ccgtcatgcg cctggttgac cacgccgatg ggcacggcct ccagccagct cccgacctcg
     4861 gcgcgcacca gcggatcgtt cgccagtccg acgtcccagt aggcgccata gccgtgaccg
     4921 agccagtagc tccgggcgat gtcgcgccgc acgaagtccc agagttcgtc gcgtcccgag
     4981 aacgaggtgt cgccgatgat ggtgcgcatg gccgcgtcga tgtcgaagtc gagtatcgca
     5041 agccagaggg cgctgccgat cagcagcagg gcgccgaaca gaccgcccgc gatgatcatc
     5101 gccgggccgt agcgctccgc cagcgcaaag gcgcagatca ccagcacggc gagcgccgtc
     5161 agattgatgg aggtcttgct cttcgtcagc accaggaaga cgagcacggg gatgagggcc
     5221 gccagcgcca gcgccttctg ccagccctcc cgcgatccca tcatccaggt gacggcgagc
     5281 acgaccgtaa tcatggcgac gatgccggcc acgttcttct gcgtgtagaa gcccttggcg
     5341 ccgatctccg acatgccgac ggatggcacg ccaagggcca ggagcaggca gatcagcacg
     5401 agcacggaaa gggtggcgaa caggacggta tggaggcgcc tgaggtcgtc cgtgctgacc
     5461 gccaccgcca gggcgatcgt cgtcaggaaa aacagcaaca tgccgcggcg cagggtgatc
     5521 ccgggaaagt tcgaccagag gatgctggcc acgcagaagc cgaccagcag gaaaagcggc
     5581 aggttggcgc cgacgcagcg cagcgcgtcg cgccagcgac cgaacagcac gaccaaggcc
     5641 accgcgaaca gcgccaggac gatcaagcga tccagcggac tgccttccga gcgcgattcg
     5701 atgctggcgc ccaggaacgg ttgcgtgccg acgaaagtga tgaacaggaa gccggcgagc
     5761 agaaaatgct ccagccacag acgcggaccg gagatctcac ggttctcgac gccgtcctcg
     5821 ggtgcgtagg agatgtggcc gctggtcgac catgcggtgg gtgtcacgaa aaccaccatg
     5881 aaaagcctgg accaagccgg ccagccttag cgaaatcgtt acctgtcgct gcgcaaggtg
     5941 gccctgaaag gtcaagaaag tctttcgacg gtcgccccag gaagcctgcc ggacatggat
     6001 accgtttcga tcgtcatccc gacctacaac cggaaccgca gcctggagcg ggcgctggcg
     6061 agcgcgctcg cgcagcagca gacctgcggc agcgtctcgc tggagatcat cgtcgtcgac
     6121 aattcaccgg acggcaacgc ggcggcgatt gtcgcagccc atgccggcgg accggtcgcg
     6181 gtgcgctccc tgcacgagcc gaagaccggc gtcgccaatg cccggaacgc cggggtagcg
     6241 gcggcgcagg gccggtgggt ggcctttctc gacgacgatg aggaagcgtc gcccgattgg
     6301 atcgcccgcc tgacggagac cgcgcgcgcc accggcgcgg acgcggtgtt cgggcctgt
//
LOCUS       DAOMXN010000151         6346 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_6466_length_6346_cov_10.046257, whole genome shotgun sequence.
ACCESSION   DAOMXN010000151 DAOMXN010000000
VERSION     DAOMXN010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 6346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6346
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6466_length_6346_cov_10.046257"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="PK812_10305"
     CDS             <1..597
                     /locus_tag="PK812_10305"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRJ69990.1"
                     /translation="VEVIILAAGRSTRMGGPNKLLAQHRGKALVRHVAESAVAAGIGP
                     VSVVVGHQADQVRAALAGLDLRFIENPAFADGLSTSLRAGIGALAPDCGAAVILLGDM
                     PLVGPAVIRRLAAAYAQTPDAKAVVPTFLGQKGNPVLIARGLFASVADVSGDIGARRL
                     IEDAGGQVVEVAVDDPGALLDIDTPQALRDLAETGTRS"
     gene            739..1059
                     /locus_tag="PK812_10310"
     CDS             739..1059
                     /locus_tag="PK812_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ69991.1"
                     /translation="MRLLFRVLSFLATLLAFVMIVVDGTTIISAKEFDYTSLGDLIGR
                     LLAPGALERGMGKLAANVHPLVARLVEVVVLAPPATINLAILALVSWMIGRQRSEPEY
                     LGER"
     gene            1197..1964
                     /locus_tag="PK812_10315"
     CDS             1197..1964
                     /locus_tag="PK812_10315"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HRJ69992.1"
                     /translation="MTPLRLAKIAGSVLILAVAIWTLARIVRIDALFEHYGAITDYVR
                     AHFGPAIALYIAIYIVVVVFSIPVALALSLIGGALFGGLAAGLAIVFAATLGAVLVFL
                     AARGLFHAYFVRKTEAWLGGIRQEFHADAASYLLILRLTPIFPFAAVNLAAALLGARL
                     GTYFWTTLVGIVPGTLAYSFVGAGLNGVLESEARRLAECRARGASPCVASFDPSAFVS
                     RDIALALGGLAVLVLLSMLARRHRSRSVAPSADSRQP"
     gene            2064..3503
                     /locus_tag="PK812_10320"
     CDS             2064..3503
                     /locus_tag="PK812_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009208217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRJ69993.1"
                     /translation="MPQPEPSNLVTTDICIIGAGAAGLSVAATTGLLGVPTVLIERGR
                     MGGDCLNSGCVPSKALIAAARAAHDIRTAGRFGIKAQPPIIERKRLMAHVQEVIDSIA
                     PTDSVARYRALGVEVIEADARFVDARTVVAGERSVRARRFVIATGAGPAIPPIPGLAD
                     VPFLTNETVFALDETPRRLVIIGGGPIGVELAQAHRRLGSEVTIIEADTLLGREDPEL
                     ADVIRMALRRDGVRLLEGARVTRVQAVSSGVTLDLEGQGAPEMLNASHLLVAIGRRPR
                     LDGLGLEAAGIAFDPRGITVDAGLKTSNRRVYAIGDCAGGPQFTHVASYQAGLVVRNA
                     LFRLPVKADYAAVPRVTYTDPELAAVGLSEAQARAEQTGVMVYRWPFAEVDRARAERE
                     TAGLIKAVVSRKGVVLGAAIAGPKAGELLAPWTLAVRTGMKIGAMADAIMPYPSLSEI
                     SKRVAVQSYAAHLRNPWVGRMIRFLRRFG"
     gene            3500..4921
                     /locus_tag="PK812_10325"
     CDS             3500..4921
                     /locus_tag="PK812_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRJ69994.1"
                     /translation="MSGAGPEPSGGARARRYFGLSGKLMLLTLAFVMLAEVLIFVPSM
                     ANFRNNWLRDRLAAARIAAFVLEAAPAEAIPETIVTQLLENVGARMIALKIEGSRRLL
                     ASSDMPLAVAREYDLRDSGPYSSTLASFDTMLHRDERLVRVIGPAPMGGDFVEVVLSE
                     RPLREAMWRYSTNILFLSLVISSITAGLVYASLHWLIVRPVKRLADSALAFSENAEDS
                     TRFITPSGRRDELGDAETALAEMQAGLSQQLKQQQHLAAMGLAVAKINHDLRNLLASA
                     HLLSDRIGTVQDPTVQRIAPKLIGALDRAVGFCQATLAFGRAQERAPAPRSFDLVALV
                     AEVSDLLGLGDEADVRVVAEMPAPMRITADPDHMMRVLLNLSRNAVDAMRQVGVSPQE
                     QGVLTISARRLKDRVEIDISDTGPGIPAEIRARLFQAFSGAARGGTGLGLAIALELMR
                     AQGGTVTLVEADRGARFRVTLPA"
     gene            complement(4922..5677)
                     /gene="soxA"
                     /locus_tag="PK812_10330"
     CDS             complement(4922..5677)
                     /gene="soxA"
                     /locus_tag="PK812_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016669 - oxidoreductase activity, acting
                     on a sulfur group of donors, cytochrome as acceptor
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019417 - sulfur oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxA"
                     /protein_id="HRJ69995.1"
                     /translation="MMRRAAVLAVLALAAPAGAEPKSGIHYQAPETRALQADDFANPG
                     MLWVERGEGMWRAADLPGGRSCASCHGNAADSMRAVAARYPAFDGQAGRVVDLEGRIN
                     LCRTVRAGGLELERESDALLALTAFVAHQSRGLAVSVRVDGPAQASFEAGRTLFETRF
                     GQLNLACRHCHVDNAGRMLRGDRISEAMPNGYPLYRLDWQKLASLQRRLRTCFAGVRA
                     EPFAYGAQELVDLSLYLAARANGLAIETPGVRR"
     gene            complement(5674..5994)
                     /gene="soxZ"
                     /locus_tag="PK812_10335"
     CDS             complement(5674..5994)
                     /gene="soxZ"
                     /locus_tag="PK812_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HRJ69996.1"
                     /translation="MATIRARLPAQARPGETVQVRALIQHPMETGFRRDEAGRAFPRN
                     ILKDFTCSYDGAVVFRAEFFPAVAADPFLAFHFVATRSGDVVLSWTEDTGKVFSQTLK
                     IDVT"
     gene            complement(6002..>6346)
                     /locus_tag="PK812_10340"
     CDS             complement(6002..>6346)
                     /locus_tag="PK812_10340"
                     /inference="COORDINATES: protein motif:HMM:NF024892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HRJ69997.1"
                     /translation="EVLRGREARPGRVRLEIPRLAESGLNVSLDFSVESPMTEADHVK
                     TVHLFAEHNPLPLIARYHFTPRSGRASVSTRIRLADSQIVTALCEMSDGSVFVDRAEV
                     VITIMACGLEPT"
BASE COUNT          929 a         2162 c         2229 g         1026 t
ORIGIN      
        1 gtggaggtga tcatcctggc ggcgggccgc tccacccgca tgggcgggcc gaacaagctg
       61 ctggcacagc accggggcaa ggcgctggtc cgccatgtcg cggagagcgc cgtggcggcc
      121 gggatcggcc cggtttcagt ggtggtggga caccaggcgg accaggtgcg ggcggcgctg
      181 gccgggctcg acctgcgctt catcgagaac ccggccttcg ccgacggtct gtcgacctcg
      241 ctcagggccg gcatcggcgc gctggcgccg gactgcgggg cggccgtcat cctgctcggc
      301 gacatgcccc tggtcggccc ggccgtcatc cgtcgcctgg cggccgccta tgcgcagacg
      361 cccgatgcaa aggccgtcgt gccgacattc ctcggccaga agggcaaccc cgtgctgatc
      421 gcgcgcggat tgtttgcgtc cgtggcggac gtttccggag acatcggtgc gcgtcgcctg
      481 atcgaggacg ccggcggcca ggtggtggag gtcgcggtgg acgatcccgg cgcgctgctc
      541 gacatcgaca cgccgcaagc cctgcgggac ctggcggaga ccggcacccg gtcctgatgg
      601 cgcgacgggc ggcggcccgt atcgcgccgt tgattgtcgc gcggggatag gatgagataa
      661 cacagagagg cccgtttcat ccgtcgcccg ccgccgaggc cgagcccgga gcggacacct
      721 cgccagattg gggtaggcat gcggctgctg ttccgggtct tgagctttct ggccacgctg
      781 ttggccttcg tgatgatcgt cgtcgacggg acgaccatca tcagcgcgaa ggaattcgac
      841 tatacctccc tcggcgacct gatcggccgg ctcctcgcgc caggcgcgct ggaacgcggg
      901 atgggcaagc tggccgcgaa cgtccatccc ctggtggcgc gccttgtgga agtcgtggtt
      961 ctggccccgc cagcgacgat caacctcgcc atcctggcgc tggtttcatg gatgatcgga
     1021 aggcagcgca gcgagccgga gtacctgggc gagcgttaga gcgtgccggc gcgccgttca
     1081 cgccactgtc acgcaggatt gcggcgcggc gcttgcgctt gcagggccgc agtgtcacca
     1141 atggccggat aacggcacgg cagtgaccgg gctgcaacgg ggagggacgg ggagccttga
     1201 cgccgctacg gctggccaag atcgctggaa gcgtcctcat cctcgcggtg gccatctgga
     1261 cgctggcgcg gatcgtgcgg atcgatgccc tgttcgagca ctacggcgcg atcaccgact
     1321 atgtccgcgc gcatttcggc ccggcgatcg cgctctacat cgcgatctac atcgtcgtcg
     1381 tcgtgttctc gattccggtg gccctggccc tgtcgctgat cggcggcgcg ctgttcggcg
     1441 gccttgcggc cggcctggcc atcgtgttcg cggcgacctt gggcgcggtg ctggtgtttc
     1501 tggcggcgcg gggcctgttt cacgcgtatt tcgtgcgcaa gaccgaagcc tggctcggcg
     1561 gcatacgcca ggaatttcac gccgacgccg cctcatacct gctgatcctc aggctgacgc
     1621 caatctttcc gttcgcggcg gtcaacctgg cggcggcgct tctcggcgcg cggcttggca
     1681 cctatttctg gacgacgctg gtcggcatcg tgcccggcac actggcctac agcttcgtcg
     1741 gcgccggact gaacggcgtg ctggaatcgg aagcgcgccg gctggcggag tgccgcgccc
     1801 gcggcgcatc tccctgcgtg gcctccttcg atccatcggc cttcgtttcc cgcgacatcg
     1861 cgctcgcgct gggcggactg gccgttctgg tcctgctctc gatgctggcc cggcgccacc
     1921 ggtcccgctc agttgccccg tcggcggatt cgaggcagcc ttagggccag gaatcgtcgt
     1981 ccgacattcc acggaccctg ccgatgccgg tgcgcccgtc ccgtgcaggg cacggcgagc
     2041 accgcagcaa ggagtggcat cacgtgcctc aacctgagcc ttcaaacctg gtgacgaccg
     2101 acatctgcat catcggcgca ggcgcggccg gactgtccgt ggcggcgacg accggattgc
     2161 tcggcgtgcc gaccgtgctg atcgagcgtg gacgcatggg cggcgactgc ctcaattccg
     2221 gctgcgtgcc atcgaaggcg ctgatcgccg ccgcacgcgc cgcccacgac atccggacgg
     2281 ccggtcggtt cggcatcaag gcgcagccgc cgatcatcga gcgcaaacgt ctgatggccc
     2341 atgtacagga agtcatcgac tccatcgcgc cgaccgattc cgtcgcccgg taccgcgcgc
     2401 tgggcgtcga ggtgatcgag gccgatgccc gcttcgtcga tgcccggacc gtggtggcgg
     2461 gcgagaggtc ggtgcgggcg cgccgcttcg tcatcgccac cggcgccgga ccggccattc
     2521 cgcccatccc cgggcttgcc gacgtgccgt tcctgacgaa cgagacagtg ttcgcgctgg
     2581 acgagacgcc ccgcaggctg gtcatcatcg gcggcggccc gatcggcgtg gaactggcgc
     2641 aggcgcatcg tcgtctcggc agcgaagtca ccatcatcga ggccgacacg ctgctcggcc
     2701 gcgaggatcc ggagctggcc gatgtcatcc gcatggcgct gcggcgcgac ggggtgcgac
     2761 tcctcgaagg cgcccgggtc acccgcgtgc aggcggtttc ctccggtgtc acgctcgacc
     2821 tggagggaca gggcgcgccg gagatgctga acgccagcca cctgctggtg gcgatcggcc
     2881 gcaggccgcg cctcgatggc ctcgggcttg aggcggcggg aatcgccttc gatccacgcg
     2941 gcatcaccgt ggatgccggc ctgaagacct ccaaccggcg cgtctatgcc atcggcgatt
     3001 gcgccggagg cccgcaattc acccatgtcg ccagctatca ggccgggctc gtggtgcgca
     3061 acgcgctgtt ccggctgccc gtgaaggccg attatgccgc cgtgccgcgc gttacctata
     3121 cggatcccga actggcggcg gtcggtctgt ctgaagcgca ggcgcgggcg gaacagacgg
     3181 gcgtcatggt ctatcgctgg cccttcgccg aggtcgatcg cgcccgtgcc gagcgcgaga
     3241 ccgccgggct gatcaaggcg gtagtcagcc gcaagggcgt ggtgctcggc gctgccatcg
     3301 ccgggccaaa ggccggcgaa ctgctggcgc cctggacgct tgcggtcagg accggcatga
     3361 agatcggcgc gatggccgac gcgatcatgc cctatccgag cctttcggaa atatcgaaac
     3421 gcgtcgccgt ccagtcctac gcggcgcatc tgcgcaaccc gtgggtcggg cggatgatcc
     3481 gctttctgag gcggttcggg tgagcggcgc cggaccggaa ccgtccggag gcgcgcgcgc
     3541 gcgccggtat ttcggcctgt ccggcaagct gatgctgctg acgctcgcct tcgtcatgct
     3601 ggcggaagtg ttgatcttcg tgccgtcgat ggcgaacttc cgcaacaact ggctgcgcga
     3661 ccggcttgcg gcggcgcgca tcgcggcctt cgtcctggag gcggcgccgg ccgaggcgat
     3721 tcccgagacg atcgtcaccc agctgcttga aaacgtcggg gcgcgcatga tcgcgctgaa
     3781 gatcgaggga tcgcggcggc tgctggcgtc ttcggacatg ccgctggcgg tggcgcgcga
     3841 atatgacctg cgcgacagtg gcccctacag ctcgacgctg gcgagcttcg atacgatgct
     3901 gcacagggac gaacggctgg tgcgcgtcat cgggccggcg ccgatggggg gcgatttcgt
     3961 cgaggtcgtc ctcagcgaac gcccgctgcg cgaggcgatg tggcgctatt ccaccaacat
     4021 cctgtttctc tccctggtga tttcctcgat cacggctggg ctggtctatg cctcgctgca
     4081 ttggctgatc gtgcgacccg tcaagcggct cgccgacagc gcgctcgcat tcagcgaaaa
     4141 cgccgaggat tccacacggt tcatcacgcc gtcgggccgg cgcgacgaac tgggggatgc
     4201 cgagacggcg ctcgccgaga tgcaggccgg gctgagccaa cagctcaagc agcagcagca
     4261 tctggccgcg atggggctgg cggtcgccaa gatcaaccac gacctgcgca acctgctggc
     4321 gtccgcgcat ctcctgtcgg accgcatcgg cacggtgcag gacccgacgg tgcaacgcat
     4381 cgcgcccaag ctgatcggcg cgctcgatcg tgcggtggga ttctgccagg ccacgctggc
     4441 cttcgggcgg gcgcaggaac gcgcgccggc gccacgcagc ttcgacctgg tcgccctggt
     4501 ggcggaagtc agcgatctgc tcggtctcgg cgacgaagcc gatgtccgtg tcgtcgcgga
     4561 gatgccggcg ccgatgcgca ttacggccga cccggatcac atgatgcgcg tcctgctgaa
     4621 cctgtcgcgc aacgccgtcg acgccatgcg acaggtcggc gtttcgcctc aggaacaggg
     4681 cgtcctcacg atctcggcgc ggcggttgaa ggaccgggtc gagatcgaca tttccgatac
     4741 cgggccgggc atcccggccg agatccgcgc ccgcctgttc caggccttct cgggagcggc
     4801 ccgcggcggc accggtctgg gtctcgccat cgcgctggaa ctgatgcggg cgcagggcgg
     4861 gaccgtcacc ctggtcgagg ccgatcgggg cgcgcgcttc cgtgtcacct tgccggcctg
     4921 atcagcggcg gacgccgggc gtttcgatcg caaggccatt ggcgcgcgcc gccagataga
     4981 gcgagagatc caccagctcc tgcgcgccgt aggcgaaggg ctcggccctg acgccggcga
     5041 agcaggtgcg caggcgccgc tgcagggacg ccagcttctg ccagtccagc cggtagagcg
     5101 gatagccgtt cggcatggcc tcgctgatgc gatcgccgcg cagcatccgg cccgcattgt
     5161 cgacatggca gtgccggcag gccaggttca actggccgaa gcgggtctcg aacagcgtgc
     5221 gccccgcctc gaacgaagct tgcgccggcc cgtcaacccg aaccgacacc gcaagccccc
     5281 gcgactggtg ggcgacgaac gccgtcagcg ccagcagcgc atccgactcc cgctccagtt
     5341 cgaggccgcc ggcccgaacg gtgcggcaca ggttgatccg gccttcgaga tcgacgacgc
     5401 ggccggcctg cccgtcgaaa gcgggatagc gcgccgcgac cgcgcgcatg ctgtccgcgg
     5461 cattgccatg acaggaggcg caggagcgcc cgcccggaag atccgccgcg cgccacatcc
     5521 cctccccgcg ctccacccag agcatgccgg gattggcgaa atcgtcggcc tggagggcgc
     5581 gtgtctccgg cgcctgataa tggatgccgg atttcggctc ggctccggcc ggcgctgcca
     5641 gcgccaggac ggcgagcacc gccgcccggc gcatcatgtg acgtcgatct tcagcgtctg
     5701 cgaaaacacc ttgcccgtgt cttcggtcca gctaagcacc acgtcgccgc tgcgcgtggc
     5761 cacgaagtgg aaggcgagga atggatcggc ggcgacagcg ggaaagaatt ccgcccggaa
     5821 gacgaccgca ccgtcatagc tgcaggtgaa gtccttcagg atgttgcggg gaaaggcccg
     5881 gcccgcctcg tcgcggcgga accccgtttc catcggatgc tggatgagcg cgcgcacctg
     5941 cacggtctcg cccggccggg cctgcgcggg aaggcgcgcg cgtattgtcg ccatggtgcc
     6001 ctcaggtggg ttccagcccg caggccatga tggtgatcac cacctccgcc cggtcgacga
     6061 agacggagcc gtcgctcatc tcgcacagcg cggtgacgat ctggctgtcg gcgaggcgga
     6121 tgcgggtgga cacgctggcg cgtccgctgc gcggcgtgaa gtggtagcgc gcgatcagcg
     6181 gcagcggatt gtgctcggcg aagagatgga ccgtcttcac gtggtcagcc tccgtcatcg
     6241 gcgactcgac cgaaaaatcg agggagacgt tgaggccgct ctcggccaat ctcggaattt
     6301 caagtcggac ccgcccgggc cgcgcctcgc gcccgcgcag gacctc
//
LOCUS       DAOMXN010000152         6333 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_6482_length_6333_cov_10.628544, whole genome shotgun sequence.
ACCESSION   DAOMXN010000152 DAOMXN010000000
VERSION     DAOMXN010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 6333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6333
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6482_length_6333_cov_10.628544"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..164
                     /locus_tag="PK812_10345"
                     /pseudo
     CDS             48..164
                     /locus_tag="PK812_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012321961.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(249..674)
                     /locus_tag="PK812_10350"
     CDS             complement(249..674)
                     /locus_tag="PK812_10350"
                     /inference="COORDINATES: protein motif:HMM:NF027482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4864 domain-containing protein"
                     /protein_id="HRJ69998.1"
                     /translation="MRIATVARLSLGLLAATVSFSAHAWEPADKAGIRKVIREQMQAI
                     SRTDSVVFASTVAPGLRSQFPNEDALYGVLVGKIPALEGAREFGFGPLKTTSVGLVQT
                     LRFTDASGNPWVSMFVMEQQDGRWFVKGVSAKQVTAQKL"
     gene            854..2959
                     /gene="flhA"
                     /locus_tag="PK812_10355"
     CDS             854..2959
                     /gene="flhA"
                     /locus_tag="PK812_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010683839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HRJ69999.1"
                     /translation="MSSGTSPRLPQPSLPSLAELRALLRRGDLALALGVLTILVVLIA
                     PLPPLLLDLLLAVSIIFSVLILMTALFIQGPLEFSAFPTILLVATMLRLALNLASTRL
                     ILANGHTGGAAAGHVIESFGAFVIGGNFAIGLIVFVILVIVNFVVITKGSTRIAEVAA
                     RFTLDAMPGKQMAIDADLSAGIISQDQARLRRKELENESAFFGAMDGASKFVRGDAVA
                     GILITIVNVLGGIAIGVLQQGLGFGEAARTYTLLTVGDGLVSQVPALIVSTAAGLLVS
                     KAGVSGSADKAFLSQLTFYPKALGMSGAVMLVIAALPGMPMLPFLLLAGGAFYVARRA
                     LAIQKAAAPQAGEAPATDADAAEEPVATALRIDELRIEIGYALLPLAQAKSGDDRLTE
                     QIKAMRRSLAADFGFVLPAVRLIDNVQLEPNRYVVRIKEIEAGAGTVFPGQFMAMDPG
                     GAPVALPGTQMLEPAFGMPVTWIDAGLREEAQFRGYTVVDAGSVIATHLTELVKAAMP
                     DLLSYVEVQKLLRDLPKEHGDLVKDIVPAAISATGLQRVLQYLLAERVSIRDLGAIIE
                     AVAEVASAMRDPRAMGEHVRARLARQICFQHADAGGRLTIIPLSAAWEDAFAGSLVGE
                     GEIRQLAMAPSKLHEFVLALKKRFEEAAQAGALPVLVTSALARPYVRMITERFRAQTV
                     VLSQAEIHASVKIETLAML"
     gene            complement(2954..4006)
                     /locus_tag="PK812_10360"
     CDS             complement(2954..4006)
                     /locus_tag="PK812_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HRJ70000.1"
                     /translation="MKKIKDFIWPLFGLVAVVWSVQLLYVKLKAEAATDARVEQMLEN
                     SSLWQSIKIIAGVIGHKLAEIPATDYLLAGLSTLAAYAALAWYDRIALLHLRKTEGIS
                     WMFISLCSFTTYALSHNIGASVFSGGAVRYRAYTSKGLTGAEVAVLVALCSFTFAFGT
                     LLLTGIVLVGEPHIVQPLLAKSPLNGMKNSVIQFVGLGILTACALYVFGSWKQLKPLV
                     IKGFRLEYPRLEVVKQQLIAAPLELIGAAGIIYFALPDVGNPGFFVVLGAFLLSFSAG
                     LLAQVPGGVGVMEAVFIIIMPQVPATAVFAALLVWRLFYLLIPLALSIPIILAFEEGR
                     LKETISHRHEDDKPQS"
     gene            4163..4780
                     /locus_tag="PK812_10365"
     CDS             4163..4780
                     /locus_tag="PK812_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRJ70001.1"
                     /translation="MTATLAASLDGITDIPAGHVATIKTYLAMPERPTDTGSTPLDRP
                     LLERLRGAQHARYRSIFATLGRRWLWWSRLQLSAGDLAAVLDDPSVEAYAVVADGADA
                     GLMEFDFREPGRADLAFLGLFEGHTGQGTGTALMAIALSALWRAGVSTVTVNTCTFDH
                     PAALAFYRRSGFSVVKQAVEIVPDPRLAGLLPRDAAPHVPLLPRT"
     gene            complement(4853..5272)
                     /gene="fliJ"
                     /locus_tag="PK812_10370"
     CDS             complement(4853..5272)
                     /gene="fliJ"
                     /locus_tag="PK812_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020180713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HRJ70002.1"
                     /translation="MKSREALLKFKKFQVEEKRRRVAQIEAMVAEFGRMAGDLDREIE
                     SEEKRAGISDPAHFAYPTYAKAARTRRDNLVNSAAELKDQLEEAKAALEVAFEDLKKV
                     EVLDDREKAAERAEAALRQQMEADRAAARMRFVVGRA"
     gene            complement(5305..>6333)
                     /locus_tag="PK812_10375"
     CDS             complement(5305..>6333)
                     /locus_tag="PK812_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0046034 - ATP metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HRJ70003.1"
                     /translation="VLDAAGCALDGRGQLPQGALAFPLRAGAPSAHSRRRLGPTLDLG
                     VRALNTFLPVRRGQRLGLFAGSGVGKSVLMSMIARNSDADVIVIGLIGERGREVQEFI
                     EDDLGPEGMKRAVVVVATSDESPLTRREAAYATMAVAEYFRDLGRHVVLMMDSVTRFA
                     MAQRDIALSIGEPPTTKGYTPTVFAELPRLLERAGTCAGEGTITAFFTVLVDGDDHNE
                     PVADAARGILDGHVVLERAIAERGRFPAVNVLRSVSRTMPGSCDPAYYPVVVEARRLM
                     ATYADMEELIRLGAYRSGTNPQVDCAIDAHEPLEKFLAQGKEESTSLSEGYSRLANIV
                     NALGSFRG"
BASE COUNT         1085 a         2191 c         1981 g         1076 t
ORIGIN      
        1 gcgccctgaa actgtgaaac atgtttccgc caaccagcag agccaggcac gccgcgcgca
       61 ttctcggcat ttccatccgc acgctgcgca acaaactcaa cgactacgag gccgccggca
      121 tcgccgttcc gcacccgggg tccgcacgcg tccaggtcgc ttgaagaagc aggccggagc
      181 gatctgcccc ggcctccctc tgccatccga acggcccgaa gaccgaggat ggcgccactt
      241 gccaagtgtc agagcttctg ggcggtcacc tgcttggcgc tcacgccctt cacgaaccag
      301 cggccatcct gctgctccat gacgaacatg ctcacccacg gattgccgga ggcgtccgtg
      361 aaccgcaggg tctgcaccag gccgaccgag gttgtcttca gcgggccgaa gccgaattcc
      421 ctggcgcctt ccagcgccgg aatcttgccc acgagcacgc cgtagagcgc gtcttcgttg
      481 gggaactggg agcgcagtcc gggcgccacc gtgctggcga agaccaccga gtcggtgcgg
      541 ctgatcgcct gcatctgctc gcggatcacc ttgcggatac ccgccttgtc agccggctcc
      601 caggcatggg cggagaacga aacggtcgcg gcgagaagtc cgagcgacag acgggcaaca
      661 gtggcgatac gcatggcact tctccctgga tcacagctcc ggcggaccgc cgccggttcg
      721 ttgagaggac attgccccgt tcgcgtgccc tgtgccgtgc gaaaacacac acgacgatgc
      781 gccgttaacc cttcgctaac cataccggcg gcaaaacttg ccgagtcaca acttctgatt
      841 gcatcggaca ccgatgagct ccggcacctc accccgcctt ccgcaaccgt ccctgccgag
      901 cctcgcggag ctgcgcgccc tgctgcggcg cggcgacctg gcgcttgccc tcggcgtgct
      961 gaccatcctc gtcgtcctga tcgcccctct gccaccgctg ctgctcgacc tgctgctcgc
     1021 cgtctcgatc atcttttcgg tgctgatcct gatgacggcg ctgttcattc agggtccgct
     1081 ggaattctcg gctttcccga cgatcctgct cgtcgccacc atgctgcggc tggcgctcaa
     1141 cctggcctcc acgcgtctca tcctggccaa cggccatacc ggcggggccg cagcgggcca
     1201 tgtcatcgag agtttcggcg cctttgtcat cggcggaaac ttcgccatcg gcctcatagt
     1261 cttcgtgatc ctggtcatcg tgaacttcgt ggtgatcacc aagggttcga cccgcatcgc
     1321 ggaagtcgcg gcacgcttca ccctcgacgc catgccgggc aagcagatgg cgatcgacgc
     1381 cgatctgtcg gccggcatca tctcgcagga tcaggcgcgg ctgcgtcgca aggagctgga
     1441 gaacgaatcg gccttcttcg gcgccatgga cggcgcttcg aagttcgtgc ggggcgacgc
     1501 cgtggccggc atcctcatca ccatcgtcaa cgtgctgggc ggcatcgcca tcggcgtgct
     1561 ccagcagggt cttggatttg gcgaagccgc gcgcacctac accctgctca ccgtcggcga
     1621 cggactggtc agccaggtgc cggcactgat cgtctcgacc gccgccggcc ttctggtttc
     1681 gaaggctggc gtctccggct cggccgacaa ggccttcctc agccagctca cgttctaccc
     1741 gaaagccctg ggcatgtccg gcgcggtcat gctggtgatc gccgcgcttc ccggcatgcc
     1801 gatgctgccg ttcctgctgc tggctggcgg cgcgttctat gtcgcgcgac gtgccctggc
     1861 gatccagaag gcagcagcgc cgcaggcagg cgaagcgccg gcgaccgacg ccgacgccgc
     1921 cgaggaaccg gtcgccacgg cactccggat cgacgaactg cgcatcgaga tcggctatgc
     1981 cctgctcccg ctggcgcagg ccaagtccgg cgacgaccgg ctgaccgaac agatcaaggc
     2041 gatgcgccgc tcgcttgcgg ccgacttcgg ctttgtcctg cccgccgttc gcctgatcga
     2101 caatgtgcag ctcgaaccca accgctatgt cgtgcgcatc aaggagatcg aggccggcgc
     2161 cggcacggtg ttcccgggtc agttcatggc catggacccc ggtggcgcgc ccgttgccct
     2221 gccaggaacg cagatgctcg agccggcctt cggcatgccg gtgacctgga tcgacgccgg
     2281 attgcgcgaa gaggcgcagt tcaggggcta taccgtcgtg gacgccggtt cggtcatcgc
     2341 cacccacctc accgaactgg tcaaggccgc gatgccggac ctcttgagct acgtcgaggt
     2401 gcagaagctg ctgcgcgacc tgcccaagga gcatggcgac ctcgtcaagg atatcgttcc
     2461 cgccgccatc tcggccaccg ggctgcaacg cgtgctgcaa tatctgctgg cggaacgggt
     2521 ctcgatccgc gatctcggcg ccatcatcga ggcggtggcg gaagtggcga gcgcgatgcg
     2581 cgatccgcgc gccatgggcg agcatgtgcg cgcccggctg gcccgccaga tctgcttcca
     2641 gcacgccgac gccggcgggc ggctcaccat catcccgttg tcggccgcct gggaggatgc
     2701 cttcgccggt tcgctggtcg gcgagggtga aattcgccag ctcgccatgg ctccatcgaa
     2761 actgcacgag ttcgtgctcg cgctgaagaa gcgtttcgag gaggccgccc aggccggcgc
     2821 cttgccggtg ctggtgacct cggcgctggc gcgcccctat gtccgtatga tcaccgagcg
     2881 gttccgcgcc cagaccgttg tgctgtcgca ggccgaaatc cacgcctcgg tgaagatcga
     2941 gaccctcgcg atgctatgac tgcggcttgt cgtcttcatg ccgatggctg atcgtttcct
     3001 tcagccgccc ctcttcgaag gccaggataa tcggaatgga gagcgccagc ggtatcagca
     3061 ggtagaacag ccgccagacg agaagcgcgg cgaacacggc ggtcgccggc acctgcggca
     3121 tgatgatgat gaagaccgct tccatcacgc cgacgccgcc cggcacctgc gccagaagtc
     3181 ccgccgagaa cgagagcagg aacgcgccca gcacgacgaa gaaccccgga ttgcccacgt
     3241 cgggcagtgc gaagtagatg atgccggcgg cgccgatcag ctccagcggc gcggcgatca
     3301 attgctgttt gaccacctcc agccggggat attcgagccg gaagcccttg atgaccagcg
     3361 gtttcaactg cttccaggag ccgaagacat acagcgcgca ggccgtcaaa atccccaggc
     3421 cgacgaactg gatgaccgaa ttcttcatgc cattcagggg ggacttcgcc agcagcggct
     3481 gcacgatatg cggttcgccg accagaacga tgcccgtcag cagcagggtg ccaaacgcga
     3541 aggtgaagga gcacagcgcc accaggaccg cgacttccgc ccccgtcagg cccttcgacg
     3601 tgtaggcgcg gtagcgcacg gcgccgcccg agaagaccga tgcgccgata ttgtgggaca
     3661 gcgcataggt cgtgaacgag cagagcgaaa tgaacatcca cgaaatgccc tcggtcttgc
     3721 gcagatgcag cagcgcgatg cgatcgtacc aggcgagcgc ggcataggcg gcgagcgtcg
     3781 aaagcccggc caggagataa tccgtcgccg gaatttcggc gagcttgtgg ccgatcacgc
     3841 cggcgatgat cttgatgctc tgccagagcg acgaattttc cagcatctgc tcgacgcggg
     3901 catccgtcgc cgcttcggct ttcagcttga catacagcag ctgcaccgac cacacgaccg
     3961 cgacgagacc gaagagcggc cagatgaagt ccttgatttt tttcattgcc cgacccgttg
     4021 cgcggctggc gccgtctgtt gccgtctgcc cgctgcttcc tgcacaaggt gtgcggcttc
     4081 cgcaaggccg aaacagcatg ccgtaccgtc cgtgcccgaa ttccgctggg atgcgacgct
     4141 gcctgcagca ggagacgctt tgatgacggc aacgctcgcc gccagcctcg atggcatcac
     4201 cgacattccg gcgggccatg tcgcgaccat caagacctac ctggcgatgc ctgaaaggcc
     4261 aacggacacc ggatcgaccc cgctcgaccg cccccttctg gagcgcctgc gcggcgcgca
     4321 gcacgcccgc taccggagca tcttcgcgac cctcgggcgg cgttggctct ggtggtcgcg
     4381 tctgcaactg agcgccgggg acctggccgc ggtcctggac gacccatcgg tcgaggccta
     4441 tgccgtcgtc gccgatggcg cggatgcagg cctcatggaa ttcgactttc gcgagcccgg
     4501 cagggccgat ctcgccttcc tcggcctgtt cgaaggtcac accggccagg gcaccggcac
     4561 tgccctgatg gcgatcgccc tgtcggcgct gtggcgcgcc ggcgtcagca cggtgaccgt
     4621 caacacctgc accttcgatc accctgcggc gctggccttc tatcggcgct ccggcttcag
     4681 cgtcgtcaaa caggcggtcg agatcgtgcc ggacccgcgg ctcgcgggcc tgctgccgcg
     4741 ggatgccgcc ccgcacgtgc cgctgcttcc ccggacatga aaacgccggg cgaagagccc
     4801 ggcgcagaca atctcatggg gcgcggcgaa gcttgcgccg cgccagcccg gttcaggcgc
     4861 gaccgacgac gaaccgcatg cgggctgcgg cgcgatctgc ttccatctgc tggcgcaacg
     4921 ccgcctcggc gcgctccgcg gccttctcgc gatcgtccag gacttcgacc ttcttcaggt
     4981 cctcgaaggc cacttcgagc gccgccttgg cctcctcaag ctggtccttc aattcggccg
     5041 ccgaattgac cagattgtcg cggcgggtcc gcgcggcctt ggcgtaggtc ggataagcaa
     5101 aatgcgccgg atcagagatg ccggcgcgtt tttcttccga ttcgatctca cggtcgaggt
     5161 caccggccat gcggccgaac tcagccacca tcgcctcgat ctgagcgacc cgacggcgct
     5221 tttcctcaac ctggaacttc ttgaatttca gcaaagcttc gcgcgacttc attgccctta
     5281 ctcaactcct gaccatttac gcggtcatcc ccggaaagag cccagggcgt ttacgatgtt
     5341 cgcgagacgg gaataaccct cgctcaagga ggttgattct tccttacctt gcgcgagaaa
     5401 tttctcgagc ggctcatggg cgtcgatggc gcagtcgacc tggggattcg tgccggaccg
     5461 ataggcccca aggcggatca attcctccat gtcggcatag gtcgccatca ggcgccgggc
     5521 ctcgaccacg accgggtagt acgcgggatc acaggagccg ggcatggtgc gcgacaccga
     5581 acgcaggacg ttcacggctg gaaaacgtcc gcgctcggcg atggcgcgct ccagcaccac
     5641 gtgtccgtcg agaatgccgc gcgcggcatc ggcgacgggc tcgttgtggt cgtccccatc
     5701 caccagcacc gtgaagaagg cggtgatggt gccctcgccg gcacaggtcc cggcccgctc
     5761 cagcaagcgc ggcaattcgg cgaaaacagt tggcgtatag cccttggtgg tcggcggttc
     5821 gccgatcgac agcgcgatgt cgcgctgggc catggcgaag cgcgtgacgc tgtccatcat
     5881 cagcaccaca tgccggccaa gatcgcggaa atactccgcc accgccatgg tggcataggc
     5941 cgcctcgcgg cgcgtcagcg gcgattcatc cgaagtcgcc accacgacga cggcgcgctt
     6001 catgccttcc ggtccgagat catcctcgat gaactcctgc acctcgcggc cgcgttcgcc
     6061 gatcaagccg atgacgatga cgtccgcgtc gctgttccgc gcgatcatcg acatgagtac
     6121 ggacttgccc acgcccgatc cggcaaacag gcccaggcgc tggccgcgac gtaccggtag
     6181 gaaggtattg agcgccctta cgccaagatc gagcgtggga ccaaggcggc gacggctgtg
     6241 cgccgacgga gcaccggccc gcaggggaaa tgccagcgcg ccttgcggca gctgaccgcg
     6301 cccgtccagc gcgcacccag ccgcatcgag cac
//
LOCUS       DAOMXN010000153         6291 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_6546_length_6291_cov_11.952694, whole genome shotgun sequence.
ACCESSION   DAOMXN010000153 DAOMXN010000000
VERSION     DAOMXN010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 6291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6291
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6546_length_6291_cov_11.952694"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(238..1218)
                     /locus_tag="PK812_10380"
     CDS             complement(238..1218)
                     /locus_tag="PK812_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HRJ70004.1"
                     /translation="MTSITITAEKLRTDCPADKFRLITRSDMDGLVCGTLLKELGLVG
                     RIDFVHPKDMQDGKIAVTSADISTNLPYVPGIYAAFDHHESEGKRVAGKPGNHVIDVK
                     APSAARVVYDFFGGKATFPRIPDAMMAAVDKADAARFNRDDVLDARGWELLSFLMDAR
                     TGLGRFREFRISNYQLMMTLIDACRNHASIEEILDLPDVRERVDLYKAHQALAREQIK
                     RCTRVEKPLAVLDLRGEETIYASNRFMIYALFPEASISCHVMWGREKQNTVLAFGKSI
                     FDRSNRTNIGELMLEYGGGGHAAAGTCQIANDRADAALSALSQRIAREAA"
     gene            complement(1501..1576)
                     /locus_tag="PK812_10385"
     tRNA            complement(1501..1576)
                     /locus_tag="PK812_10385"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1541..1543),aa:Ile,seq:cat)
     gene            complement(1629..2141)
                     /locus_tag="PK812_10390"
     CDS             complement(1629..2141)
                     /locus_tag="PK812_10390"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRJ70005.1"
                     /translation="MADPATFTIQPLTPGTRGPAKALLRRVFTDEEWFDELNIALLGT
                     RFQGFSRLLGVEGQRVWVAVAPHGAVLGVTGLYIVSGDADAVWLGWFCVAPEARGQGV
                     GGALLDFSIEEARRSGRPFLRLVTSTDASEAAAQGLYESRGLTVVARRQPFSWIGSGV
                     EELTRQRSLS"
     gene            complement(2134..2547)
                     /locus_tag="PK812_10395"
     CDS             complement(2134..2547)
                     /locus_tag="PK812_10395"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018043079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HRJ70006.1"
                     /translation="MPKTDQSEPRGELAIRTIAMPADTNANGDIFGGWVLARMDQAGG
                     VMGVSFSKGRVVTASLQAMSFLKPVQVGDVLSVYTEVTRVGRTSFDLRVEAWARRFLT
                     REHVKVTEGTFTFVAIDDAGKPRVLPQAAMPGGHG"
     gene            complement(2602..3831)
                     /locus_tag="PK812_10400"
     CDS             complement(2602..3831)
                     /locus_tag="PK812_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006939849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRJ70007.1"
                     /translation="MSAADYSHHLPDSRYAWMRLVASLVVGTMACAGIWTVVVVIPTV
                     QEEFGTGRAGASLPYTLMMVGFGAGTILMGRVADRTGMVVPIIASALMLGVGYVLAGL
                     AGSLTSFALVHGLLIGTGAAAGFGPLMADVSHWFVKRRGLAVVLAACGSYFAGTLWPL
                     VMNWAIPQWGWRATHVGIGIAIVVVMLPVALMFRAQPAKATLADAETASDAARADLGL
                     SPGALQGLLVIAGLSCCVAMSMPQVHIVAYCNDLGYGVARGAEMLALMLGLGVVSRVL
                     SGFLADRIGGPGTLAVGSFMQAVALLLYLFFNGLSSLYVISAIFGLFQGGIVPMYAVI
                     AREFLPPREAGLRIGLIISATIAGMAVGGYLSGVIFDYTASYRMAFLHGFLWNLLNLA
                     LAGWLWWKRLGPRLATA"
     gene            complement(3910..4194)
                     /locus_tag="PK812_10405"
     CDS             complement(3910..4194)
                     /locus_tag="PK812_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyU family transcriptional regulator"
                     /protein_id="HRJ70008.1"
                     /translation="MSFLKKLFGLGGDGGGAAAQAPAQEHNGFTVRAMPYQEGGQWQM
                     CGVISKEINGEVKEHRFIRADRFPGREDAVEFTFAKARQIIDMNGEKIFK"
     gene            4807..6270
                     /locus_tag="PK812_10410"
     CDS             4807..6270
                     /locus_tag="PK812_10410"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRJ70009.1"
                     /translation="MPTTDDLVASLQKLRRTETLGALPQALDRLARLAVELFDADLAA
                     LAFHRGGVSRVVASSGLEARFRSLTWDFAAVRYAPDERVVLPDATKNQDASAVRRFLG
                     LDAVGFLLREPIDTHPDHSLALIILGREARSRPSRRQLKLLAEIVDAIRTTAAPHLRQ
                     LAAAHPSATTALSLNEAYAAVETAPWPAALFDSRQHVLALNSEMARLIGRDAERAIGK
                     APDELRLPMGGAIMQLQEQALRTGLSPPDVEVISPADSGRRAFQLHLSPFSPTDDAQT
                     YLYAAAREVTDGIVQERQIAERIRLRRPDSPSAPVSFLLETLVARRAIRNRKAMQYLT
                     LRSWRSNLKSWQIKTLRALKADIPPELPDRVADEILADVTALFGLGAFHAIVPVPCGH
                     SGEGPCFSLEIARALALRSGIPVARALESRPQRGSSHPKENVKRPPMVLAQRLSGPAL
                     IVDDVATSGSHLEEAVTLLRPGCGSTLAVAWIGAGVG"
BASE COUNT         1110 a         2187 c         1993 g         1001 t
ORIGIN      
        1 ggttgctgcc gagcagcgag gcctttttga cgggcatggg caggcgcggg atgacaggcg
       61 tcgagtgcgg cacagcgcgc cagcgacggt ccccgcgcca aggccagaga cgaaaaggcc
      121 gcgtgcgaca cgtaccgtcg cacgcggccg aacatgtttt ccggggaccc ggcgcgggaa
      181 tgtcggggga agatccggcc tcgccgaagc cggtctctcc cgcaaccgct ggatggatca
      241 ggccgcttcc ctggcgatgc gctggctcag ggcgctcagc gccgcgtcgg cccggtcatt
      301 ggcgatctgg caggtgcccg ccgccgcatg gccgccgcca ccgtattcca gcatcagttc
      361 gccgatgttg gtccggttgg agcgatcgaa gatcgacttg ccgaaggcga ggaccgtgtt
      421 ctgcttttcc ctgccccaca tcacgtggca ggagatgctc gcttccggga acagcgcata
      481 gatcatgaag cggttggagg catagatcgt ctcctcgccg cgcagatcga gcactgccag
      541 cggcttttcg acacgcgtgc agcgcttgat ctgctcgcgc gccaacgcct gatgcgcctt
      601 gtagaggtcg acgcgctcgc gcacatccgg cagatcaaga atctcttcga tcgaggcgtg
      661 atttcggcag gcgtcgatca gcgtcatcat cagctggtag ttcgaaattc ggaactcgcg
      721 gaagcggccg aggccggtgc gggcgtccat caggaaggac agcagttccc agccgcgcgc
      781 atccagcacg tcgtcccggt tgaaccttgc ggcatccgcc ttgtcgaccg ccgccatcat
      841 ggcgtcggga atgcgcggga acgtcgcctt gccgccaaag aaatcataga cgacgcgggc
      901 cgccgagggc gccttgacgt cgatcacgtg gttgccgggt ttgccggcga cgcgcttgcc
      961 ctcgctttcg tgatgatcga aggccgcgta gatgccgggg acatacggca ggttcgtcga
     1021 tatgtcggcg ctggtgacgg cgatcttgcc gtcctgcatg tccttgggat gcacgaaatc
     1081 gatcctgccg acgagaccga gttccttcag cagcgtgccg cagaccaagc cgtccatgtc
     1141 gctgcgggtg atcaggcgga acttgtccgc cgggcagtcc gtgcgcagct tctcagccgt
     1201 gatcgtaatc gatgtcatga tgcgatgctt ccccgttggt tgttgcctct cgcgggcggg
     1261 aagactggcg tttcgaggta aagaactcgg ttacgacccg gacgccgaca gccggaaaag
     1321 ccggactggt taagcaaacg tcgcgttgat cgatcaagtc gcacgcgact tccggttcac
     1381 ggagctgtct gcgagctcgc gccggcgagc cgccgcgcgg taccggacgc catgacgacc
     1441 acgccgctca tcaacgcgag atgtcaagaa ttccaatgac ttcaaggtgg gcagacatac
     1501 tggtgggccc tgcaggactc gaacctgcaa ccagaccgtt atgagcggtc ggctctaacc
     1561 attgagctaa gggccctcgc gccgaaataa ggccttgccg gccacgctgg caaggggctt
     1621 gcgcatcctc acgacagcga gcgttggcgc gtcaattctt ccacaccgct gccgatccag
     1681 ctgaagggct ggcgacgggc caccaccgtg agtccgcggc tttcgtagag accctgcgcc
     1741 gccgcttcgc tcgcatcggt cgaggtcacc aggcgcagga acggtcgacc tgaccgcctt
     1801 gcctcctcaa tcgagaaatc gagcaacgcg ccgccaacgc cctgtccgcg cgcttccggc
     1861 gcaacgcaga accagcccag ccagaccgcg tccgcatcgc cgctgacgat gtacagcccg
     1921 gtgacgccga gaaccgcgcc atgcggggca acggcgaccc acacccgctg cccctcgaca
     1981 ccgagcagcc gggaaaaacc ctgaaagcgc gtgccgagca gcgcgatgtt cagctcgtcg
     2041 aaccattcct cgtcggtgaa cacccggcgc aacagcgcct tcgccggacc ccgcgtcccg
     2101 ggcgtcagcg gctggatggt gaaggttgcc ggatcagcca tgcccgccgg gcatcgccgc
     2161 ctgcggcagc acgcgcggct tgccggcgtc gtcgatggcc acgaaggtga aggttccctc
     2221 ggtgactttc acatgttcgc gcgtcaggaa ccgccgcgcc caggcctcga ccctcaggtc
     2281 gaaggacgtg cggccgacgc gcgtcacttc ggtgtagacc gagaggacat cgcccacctg
     2341 caccggcttg agaaagctca tggcctgcag cgaggcggtg acgacccggc ctttcgagaa
     2401 cgagacgccc atcacaccgc cggcctggtc catcctcgcc agcacccagc cgccgaaaat
     2461 gtcgccgttg gcgtttgtgt ccgccggcat ggcgatggtg cggatggcaa gctcgccgcg
     2521 cggttcgctc tggtcggtct tcggcatgcg ggtacggccc ctccctgatc ggccgcaacc
     2581 tgccagccaa ccgccccgga ttcaagcggt ggcgagacgc ggccccagcc gcttccacca
     2641 cagccagccg gcgagcgcga ggttgagcag gttccacagg aacccatgca ggaaggccat
     2701 gcgataggag gcggtgtagt cgaagatcac gcccgagaga tagccgccca ccgccatgcc
     2761 ggcgatggtg gcggaaatga tcaggccgat ccgcagaccg gcctcgcgcg gcggcaggaa
     2821 ttcgcgcgcg atgaccgcat acatcggcac gatgccgccc tggaacaggc caaagatcgc
     2881 ggaaatgacg tagagcgacg acaggccgtt gaagaacagg tagagcaaca gcgcgaccgc
     2941 ctgcatgaac gagccgaccg ccagcgttcc cggcccgcct atccggtcag ccaggaagcc
     3001 ggacaggacg cggctcacca cgccaaggcc gagcatcagg gccagcattt ccgccccgcg
     3061 cgccacgccg tagccaaggt cgttgcaata ggcgacgata tgcacctgcg gcatcgacat
     3121 ggcgacgcag caggatagcc cggcgatcac cagaaggccc tgcagcgcac cgggcgacaa
     3181 accgaggtcc gcccgcgccg cgtcggaggc ggtctcggca tcggccagcg ttgccttggc
     3241 cggctgcgcc cggaacatca gcgccaccgg cagcatcacc accacgatgg cgatgccgat
     3301 gccgacatgc gtcgcccgcc agccccattg cgggatcgcc cagttcatca ccagcggcca
     3361 cagcgtgccg gcgaaatagg agccgcaggc ggccagcacc acggccagcc cccggcgctt
     3421 cacgaaccag tgcgacacat ccgccatcag cggaccgaag ccggccgcgg cccccgtgcc
     3481 gatgagcaag ccgtgcacca gcgcgaagct cgtgagcgag ccggcgagac cggccaggac
     3541 gtaccccaca cccagcatca gcgcggaggc gatgatcggc acgaccatgc cggtgcgatc
     3601 ggcgacccgg cccatcagga tggttccggc gccgaacccg accatcatca gcgtgtaggg
     3661 cagcgaggcc ccggcccgtc cggtgccgaa ctcctcctgc accgtcggaa tcaccaccac
     3721 caccgtccag atgccggcac aggccatggt gccaacgacg agcgaggcga caaggcgcat
     3781 ccaggcatag cgggaatcgg gcaggtgatg ggaatagtcg gcagcgctca tgccatcagc
     3841 atcgcggcta gggcgcggcg ttgtcaaatg attagaacgc gaaggatcga tccgtcccga
     3901 tgatgcgttc tacttgaaga ttttctcgcc gttcatgtcg atgatctggc gcgccttggc
     3961 gaaggtgaat tccaccgcat cctcgcggcc gggaaaccga tcggcgcgga tgaagcgatg
     4021 ctccttcacc tcgccgttga tctccttgga gatgacgccg cacatctgcc actgcccacc
     4081 ctcctggtag ggcatcgcgc gaacggtgaa gccattgtgc tcctgcgccg gggcttgcgc
     4141 cgccgcgccg ccaccatcac cgccgaggcc gaaaagcttc ttcaagaagg acattcaccg
     4201 ctcctgctca tcggtcagcg ctgatcgccg cgccccaccc tgcgcgcatc atcgccattc
     4261 cggtcccgga tgcaagacgc gcgctcgcgc ccgccgccct cgggcggcag gatcggggaa
     4321 accggcgcgg gcacgaggcc gcggacgttc ccgtcccgag gcctctcaaa gcgatcgttc
     4381 attgcacgtg cggggacaac cttgatcccg cacccgcgcc gcggcgtttg tcagcctgct
     4441 cgcacgtcct cgccgggact gccctcccgg ccattgcggc ccgcggcgat gcagggccaa
     4501 ggacgccgtt tcggtctgcc cctacgcacc ccggacgcat ctccgggctg cgacggacgg
     4561 tgtcatcgcg gcgtgccgcg acagggaccg tgcgcatggg tcgccggccc ccgtccgcat
     4621 cgatgcgctg cagacgcttc cccatggacg ggagcgatgg caaaaattgt aggaattaat
     4681 tccggattgt caagtccttg gccaccgccg attcgccagc attgcaacaa tcggctcgct
     4741 ggggcagttt gcgccagtga acggtcccca tgcggtcggg ccaccgcaca gatccagcga
     4801 agcgccgtgc caacaaccga tgacctggtc gcctcgctgc agaaattgcg gcgaaccgag
     4861 actcttggtg cgctgccgca ggcgctggat cggcttgcgc gcctcgccgt cgaattgttc
     4921 gatgccgatc tcgccgccct ggcattccat cgcggcggcg tgagtcgcgt cgtcgcgagc
     4981 agcggcctcg aagcgcgatt tcgcagcctg acctgggact ttgccgccgt gcgctatgcg
     5041 ccagacgagc gtgtggtgct tcccgatgcc acgaagaacc aggacgcttc ggctgttcgt
     5101 cgatttctcg gtctcgacgc ggtcggtttt ctgcttcgtg agccgatcga cacgcatccc
     5161 gatcattcgc tggcgctcat catcctgggg cgcgaagccc ggtcgcggcc atcgcgcagg
     5221 caattgaaac ttctggccga gatcgtcgat gcgatccgca cgacggcggc gccgcatctg
     5281 cgccaactcg cggcggctca cccctccgca acgacagccc tgagcctgaa cgaagcttat
     5341 gctgcggtcg aaacggcccc gtggcccgcc gccctgttcg acagtcgtca gcacgtcctc
     5401 gctctgaatt ccgaaatggc ccgcctgatc ggccgagacg cggagcgcgc catcggcaaa
     5461 gcgccggacg agctgcgctt gcccatgggc ggggccatca tgcagttgca ggaacaagcg
     5521 ctgcgcaccg ggctgtcgcc gccggacgtc gaagtcatct ctcccgccga cagcgggcgc
     5581 cgggcattcc agctccacct gtcgcccttt tcgcccacgg acgatgcgca gacctacctc
     5641 tacgccgcag cgcgcgaagt caccgacggc atcgtacagg agcgccagat cgcagaacgg
     5701 atcaggctgc gccgaccgga cagcccatcc gcaccggtgt ccttccttct cgagacgctc
     5761 gtggcccgcc gtgccatccg caaccgcaag gccatgcagt acctcacctt gcgatcctgg
     5821 cgaagcaatc tgaaatcctg gcagatcaag acgttgcgcg ccctcaaggc cgatattcct
     5881 ccggagcttc ccgatcgcgt cgctgatgaa attctggccg atgtcacggc cctgttcggc
     5941 ctgggcgcgt ttcacgccat cgttcctgtc ccctgcggcc attccggcga gggcccctgt
     6001 ttctcgctgg agattgcccg cgcgctcgcc ttgcgctccg gcatcccggt agcgcgtgcc
     6061 ctggaaagcc gcccccagcg cggctcctcg catccgaaag agaacgtcaa acgcccgccg
     6121 atggtgctgg cacagcggct ttccggcccg gcgctgatcg tcgacgacgt ggcgacatcc
     6181 ggctcccacc tcgaggaagc ggtgacgcta ctgcgcccgg gctgtggctc gaccctggcg
     6241 gtggcgtgga tcggcgcggg ggttggctga cccggctcat accaagggtc a
//
LOCUS       DAOMXN010000154         6252 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_6599_length_6252_cov_9.720026, whole genome shotgun sequence.
ACCESSION   DAOMXN010000154 DAOMXN010000000
VERSION     DAOMXN010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 6252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6252
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6599_length_6252_cov_9.720026"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..454)
                     /gene="fliN"
                     /locus_tag="PK812_10415"
     CDS             complement(140..454)
                     /gene="fliN"
                     /locus_tag="PK812_10415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HRJ70010.1"
                     /translation="MSQTTLSPPPALPSLASPPDRQSDDTDLRRLNDLPVKVSAVVGR
                     ARMAIGDLMKAGPGTIVELDRKAGAPVDILVNDRLVARGEIVLIDGQLGVTLTELLQE
                     GA"
     gene            complement(451..1065)
                     /locus_tag="PK812_10420"
     CDS             complement(451..1065)
                     /locus_tag="PK812_10420"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HRJ70011.1"
                     /translation="MTMPTFEPVAFGMLRASRSDIGACDLAQPSPEAAAAYAQGFEDG
                     QQTEAAVRAARTAAALDSLAQSLESFRSALSELQDEMRADAARLALSFARKLAGRLIE
                     AEPVAPVLEALASLAFDLAQREDVTIHVGPDALPALQAALEASQPAPRIAPILVADPD
                     LTTGDFRIDWPDGGLIRDRNAIEARLEAILDRSLSPQSAPEVPS"
     gene            complement(1062..2057)
                     /locus_tag="PK812_10425"
     CDS             complement(1062..2057)
                     /locus_tag="PK812_10425"
                     /inference="COORDINATES: protein motif:HMM:NF013838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HRJ70012.1"
                     /translation="MRPATGAERAALVLIALGEKHGAQLWQHLDDGDIEAVSTAMVRL
                     GRVDETYVNAAIDLFAGSLQAGAVTGNVGSAEKLLRGVLPEDRVAPILERIRRPDGPN
                     VWERLSALNAQVLADYLSGEHPQTVAVVLARLPAERSATVIERLEPAFALQCLERMAR
                     PSTVDGQTLVAVEQALGAGLAEVTAGSAQSDPAASIADIFNALDRRAGDDLLGRWTAA
                     DADTAARIRSKMFTFDDFAKLDAAAAQALLRVVDRELLAVALKGAGEPLRAFFMNQMS
                     SRAARMFEDQMSARGPMRRSEVEQARIRIVGLAKELIASGEIRLPTDDAAEDVIE"
     gene            complement(2054..3634)
                     /gene="fliF"
                     /locus_tag="PK812_10430"
     CDS             complement(2054..3634)
                     /gene="fliF"
                     /locus_tag="PK812_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012255237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HRJ70013.1"
                     /translation="MQGLIDFVQKLGPQRLVAMGAVTLALAAFFGFIAFRVAQQPAGI
                     LYADLAATESAAIARELDTRGIRYELRNDGATIAVPRDLIPRLRMDLAAKGIPAGGSV
                     GYEIFDKGDAFSATGFMQNLNQVRALEGELARSIRALERIQAARVHLVLPERRLFQKD
                     RSEARASVVLRVRGELEAGQVRAVRHLVASAVDGLKPARVSLIDEAGRLLADGAGDET
                     PGGVAAEERQQGFERRLRGQIEDIVASVVGRSRARVQVSAEFEHNRVQQTSETYDPES
                     RVIRSTQSRTESNATTEGKDGQVSIGNELPGAQKPDGAAGAREQGQKQEETVNYEISR
                     TTRTETLEAGRLKRLSVAVLVDGVHAKGANGEATYTPRPAEELQRIAALVRIGIGFDK
                     ARGDQVEVVNLRFAETPPPLEVREPTLLERLQMLARDEMLRIAEIGVFAVLSLVALIF
                     IVRPLVRQMLAPAEPVAPPGVPTVRPADGTTVSGAEAPGMATGLPPPNAIERLGERIA
                     SQPQEAAAILKTWIRSAA"
     gene            3747..4739
                     /gene="pdxA"
                     /locus_tag="PK812_10435"
     CDS             3747..4739
                     /gene="pdxA"
                     /locus_tag="PK812_10435"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006334128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HRJ70014.1"
                     /translation="MNLDKQSLIAITMGDAAGVGPEIIAKFFAQRPGVPAFVIGDVGV
                     MTRAATLAPGCPPVRPVASPDDARGAILPVLPVSDLPADLAPGRVDAGAGRAAYDAIL
                     AAIDLALDGRIRAMVTAPIHKEALAAAGIRHPGHTEILAERTGTEHFGMMLTSAHLRV
                     VLVSIHVPLAEAIRLVTRDRVVAAIRLADEGVRAFGVARPRIAVAGLNPHAGEGGLFG
                     REEIDIIAPAVAEAQAEGFDVTGPHAPDTVFMRARAGAFDAVVAQYHDQGLIPVKYLG
                     IEGGVNITVGLPIVRTSVDHGTAFDIAWQGIADAGSLGAAYDAAVVLAERRQTT"
     gene            complement(4786..5061)
                     /locus_tag="PK812_10440"
     CDS             complement(4786..5061)
                     /locus_tag="PK812_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1153 domain-containing protein"
                     /protein_id="HRJ70015.1"
                     /translation="MTEPYRPRAKYVIGPDGSPLTIADLPPSNTRRWVIRRKAEVVAA
                     VRGGLLSLDEACSRYTLTVEEFLSWQQSIDDHGLAGLRTTRIQQYRA"
     gene            complement(5160..5825)
                     /locus_tag="PK812_10445"
     CDS             complement(5160..5825)
                     /locus_tag="PK812_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HRJ70016.1"
                     /translation="MSTALTNTASATSGTAAGGTRASIAQNFDAFLGLLTTQLKNQSP
                     MDPLDTNQFTQQLVQFAGVEQQLKTNESLSALVTSSRASMVTSALGFVGNTITADGAT
                     SRLSGGKAEWRLQAPRSAAGAVLSVTDKNGSIVFTETRGLAAGEQSFVWRGRTSSGAV
                     AADGDYKLTVTARDASGQTIAVKTEIAGVVTGVDVSGAEPVLNIGSLSVPVSMVKTIQ
                     TAK"
     gene            complement(5844..>6252)
                     /locus_tag="PK812_10450"
     CDS             complement(5844..>6252)
                     /locus_tag="PK812_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HRJ70017.1"
                     /translation="QVPVLIAGMARDGARRFEIRLEPEDLGRVDVRLDIDRDGQVRAH
                     LVVERPEALAALRREAHALQQAFAETGLRSDGTGIQVSLRQGGDGQHHAAYRHAHTEL
                     ADDPMNRSAEDQSQPGAGTARRLALRSAIDIML"
BASE COUNT          956 a         2052 c         2185 g         1059 t
ORIGIN      
        1 gccgcgcccg gaacgcatgt gcgccagcgc cgcgtccagg gtgtcggcct gtgcgacctg
       61 cgcgccgcgc tcgcgggcga tcttggcggc ggcgatgagt tcgccccgga aggggcccac
      121 gatcagcagg cgcatggggt cacgctcctt cctgcagcag ttcggtgaga gtgacgccga
      181 gctggccatc gatcaggacg atctcgcctc gggccaccag gcggtcgttg acgaggatat
      241 ccaccggcgc gccggccttg cggtcgagtt cgacgatcgt tcccgggccg gccttcatca
      301 gatcgccgat tgccatgcgc gcgcggccca ccacggcgga aaccttgacc gggaggtcgt
      361 tcaggcggcg caggtcggtg tcatcgctct ggcggtcagg gggtgaggcc aggctcggca
      421 aggccggcgg cggtgacagg gtggtttggc tcatgacggg acctccgggg cggactgggg
      481 tgaaaggctg cggtcgagga tcgcttccag acgcgcttcg atggcgtttc ggtcgcggat
      541 gagtccgccg tccggccagt cgatccggaa gtctccggtc gtcagatccg ggtcggcgac
      601 gaggattggc gcgattcttg gggctggctg cgacgcttcc agcgccgcct ggagcgcggg
      661 cagggcatcc ggcccgacgt ggatggtgac gtcttcgcgt tgcgccaggt cgaaagcgag
      721 cgatgccaag gcctccagca ccggcgcgac gggttccgcc tcaatcagtc gaccggcgag
      781 cttgcgcgcg aaggacagtg cgagacgggc agcgtcggcg cgcatctcgt cctgcagttc
      841 ggacagggcg gaacggaacg attcgagcga ctgggcaagg gaatcgagag cggccgcggt
      901 cctggcggcc cgcaccgccg cctcggtctg ctgtccgtcc tcaaaccctt gcgcataggc
      961 tgcggcggct tccgggctgg gctgagcgag gtcgcaagcc ccgatgtcgg agcggctggc
     1021 gcggagcatt ccgaaggcga caggctcgaa ggtgggcatg gtcattcgat gacatcctcg
     1081 gcggcatcat ccgtcggcag gcggatttcc ccggacgcaa tcagctcctt ggccaggccg
     1141 acgatgcgga tgcgcgcctg ctcgacctcg ctccggcgca tggggccgcg cgccgacatc
     1201 tggtcctcga acatccgcgc cgcccgcgag gacatctggt tcatgaagaa ggcgcgcagg
     1261 ggttcgcctg ctcccttgag cgcgacggcc agcagttcgc ggtcgaccac gcgcagcagt
     1321 gcctgtgccg ccgccgcgtc gagttttgcg aagtcatcga aggtgaacat tttggaacgg
     1381 atgcgcgcgg cggtgtcggc gtcggcggcg gtccagcggc cgagcaggtc atcgcccgcc
     1441 cgacggtcga gcgcgttgaa gatgtccgca atgcttgcgg cgggatcgga ctgggcgctg
     1501 ccggcggtca cctccgcgag ccctgcgccg agagcctgtt cgactgcgac cagagtctgc
     1561 ccgtcgaccg tgcttggacg cgccatccgt tcgaggcatt gcagcgcgaa agccggttcc
     1621 agccgttcga tgactgtggc ggatcgctcg gccggcaggc gggcaagcac gactgcgacg
     1681 gtctgcgggt gctcaccgga caggtagtcg gccagaacct gcgcgttcag ggcggatagc
     1741 cgctcccaga cattgggccc gtccgggcgc cgtatccgct ccaggatcgg ggccacccgg
     1801 tcttccggga gcacgccacg caggagtttc tccgcactgc caacattgcc ggtcaccgct
     1861 ccggcctgga gtgacccggc gaacaggtcg atcgccgcat tgacataggt ctcgtcgacg
     1921 cggccgagcc ggaccatggc ggtcgaaacg gcttcgatat cgccatcgtc cagatgctgc
     1981 cagagctggg caccgtgctt ttcacccaga gcgatgagca cgagcgccgc gcgttcggcg
     2041 ccggtcgcgg gtctcatgcg gcgctcctga tccatgtctt caggatcgcc gcggcttcct
     2101 gcggctggct ggcgatgcgc tctccgagcc gttcgatggc attgggcggt ggcaggccgg
     2161 tcgccatgcc gggggcctcc gcgccagaaa cggtcgttcc gtcagccggg cgcactgtcg
     2221 gaacgcctgg cggcgccacc ggctcggccg gcgccagcat ctggcggacc aggggccgca
     2281 cgatgaagat cagcgctacc aacgagagca cggcgaaaac gccgatctcg gcgatccgca
     2341 gcatctcgtc gcgggcgagc atctgcaggc gttcgagcag cgtcggctca cgcacttcga
     2401 gcggaggagg ggtctccgcg aagcgcaggt tgacgacctc gacttgatcg ccgcgcgcct
     2461 tgtcgaagcc gatgccgatg cgcaccagcg cagcgatacg ttgcagttct tcggccggac
     2521 gcggcgtgta ggttgcctcg ccattcgcgc ctttggcatg gacgccgtcg accaggacgg
     2581 cgacggacag gcgcttcagc cggccggcct caagcgtctc ggtgcgcgtg gtcctggaaa
     2641 tctcgtagtt gacggtttct tcctgcttct gaccctgctc gcgggcgccg gctgcgccat
     2701 ccggcttctg cgcgcccgga agttcgttgc cgatgctgac ctgcccgtcc ttgccttcgg
     2761 tcgtggcatt ggattcggtg cggctctggg tggaacggat cacccggctc tcggggtcgt
     2821 aggtctccga agtctgctgg acccggttgt gctcaaattc ggcggatacc tgcacccgtg
     2881 cccggcttcg cccgaccacg ctggcgacga tgtcctcgat ctggccacgc aggcgccgct
     2941 cgaatccctg ctggcgctct tccgccgcga cgccgcccgg ggtttcgtcg ccggcgccat
     3001 cggccagcag gcggccggcc tcgtcgatga gcgagacgcg ggcgggcttc aggccgtcca
     3061 cggcggaggc gacgagatgg cggacggcgc ggacctgtcc agcctccagt tcgccgcgca
     3121 ccctgagcac gaccgaggcg cgtgcttcgc tcctgtcctt ctggaacagc cgccgttccg
     3181 gcagcacgag gtgaacccgc gcagcctgaa tgcgttccag cgcgcggatc gaacgcgcca
     3241 gttcgccttc gagcgcacgc acctggttga gattctgcat gaagccggtg gcggagaacg
     3301 catcgccctt gtcgaaaatc tcatatccga cgctgccgcc ggccgggatg cccttggcgg
     3361 cgagatccat gcgcaggcgc gggatcaggt cgcgcggaac ggcgatcgtc gcgccgtcgt
     3421 tgcgcaattc atagcggatg ccgcgcgtat caagttcgcg cgcgatggcg gccgattcgg
     3481 tcgcggcgag atcggcatac aggatgcctg cgggctgctg ggccaccctg aaggcgatga
     3541 agccgaagaa tgcggccagc gccagcgtca ccgcgcccat cgcgacgagg cgttgcgggc
     3601 caagcttctg gacgaaatcg atcaatccct gcacagtctt caaaccccgg aaacggacgc
     3661 tcgcaagagc gctctcggca atttctgccg cccggctggt taccaaattc ttaatgaacg
     3721 caatccaggg ttgaaaaaat caaactatga atctcgacaa gcaatccctg atagcgatca
     3781 ccatgggcga tgcagccggt gtgggtccgg agatcatcgc gaagttcttt gcccaaaggc
     3841 ccggcgttcc ggccttcgtg atcggcgatg tcggggtcat gacacgggcg gcgaccctcg
     3901 cgccggggtg tccgccggtc cggccagtcg cctcgccaga cgacgcgcgg ggtgccatcc
     3961 tgccggtgct tcctgtcagc gatctgccgg ccgatctcgc gccgggccgg gtcgacgccg
     4021 gcgccggcag ggcggcctat gacgcgattc tcgcggcgat cgatctggcg ctggacgggc
     4081 ggatcagggc gatggtgacg gctccgatcc acaaggaggc gctggcggcc gccggcatcc
     4141 gtcatcccgg ccataccgaa atcctcgccg agcgcaccgg taccgagcac ttcggcatga
     4201 tgctgaccag cgcgcatctg cgcgtcgtcc tggtgtcgat ccatgtgccg cttgccgagg
     4261 cgatccggct ggtgacacgc gatcgcgtcg tcgcggcgat ccggctcgcg gacgaaggcg
     4321 tgcgcgcctt cggggtcgcg cggccccgca tcgcggtggc ggggctgaat ccgcatgccg
     4381 gcgaaggcgg gctgttcgga cgcgaggaga tcgacatcat tgccccggcg gtggccgagg
     4441 cgcaggccga ggggttcgat gtgaccgggc cgcatgcgcc cgacaccgtt ttcatgcgcg
     4501 cgcgcgccgg cgccttcgac gcggtggtgg cgcaatacca cgatcagggt ctgatcccgg
     4561 tgaaatatct cggcatcgaa ggcggagtga acatcacggt cggcctgccg atcgtgcgca
     4621 ccagcgtcga tcacggcacg gccttcgaca ttgcctggca gggcatcgct gacgccggca
     4681 gtctgggcgc cgcctatgac gcggccgtgg tcctggccga gaggcggcag acaacgtaaa
     4741 acgccgcggc ggagccacgg cgttgaaatg cttcggtttt cgcgatcagg cgcggtactg
     4801 ctggatccgc gtcgtcctga ggccggccag gccgtgatcg tcgattgact gctgccagga
     4861 caggaattcc tcgaccgtca gggtgtaccg cgaacaggcc tcgtcgaggg agagaagtcc
     4921 accgcgtacc gcggccacca cctccgcttt tcggcggatc acccagcgcc tggtattgga
     4981 aggcggcagg tccgcgatcg tcaaagggct gccatcgggg ccgatgacat acttcgcgcg
     5041 cgggcgatat ggttcggtca tgggtacgct cacaggacac aacgcaacaa actgcaagca
     5101 ccatagatcg gcgcggctta aaaaatggct aagctgcggc aaacattgct gaataatagt
     5161 catttcgctg tttggattgt cttaaccatg ctgaccggca cgctcaggct tccgatgttc
     5221 aggacgggct ccgctcccga gacgtcgacg cccgtaacga cgcccgcgat ttcggtcttc
     5281 acggcgatgg tctggcccga agcatcgcgc gccgtgacgg tcagcttgta gtcgccgtcc
     5341 gccgccacgg cgccggacga ggtgcggccg cgccagacga aggactgctc gcccgccgca
     5401 agtccgcgcg tctcggtgaa gacgatgctg ccgttcttgt cggtcacgga caggacggcg
     5461 cctgccgccg aacgcggcgc ctgcaggcgc cattcggcct tgccgccgga caggcgcgat
     5521 gttgccccgt ccgccgtgat cgtgttgccg acgaagccaa gcgccgatgt caccatactg
     5581 gcgcggctcg atgtgacaag cgctgaaagg ctctcgttcg tcttgagttg ctgctcgaca
     5641 cccgcgaact ggaccagttg ctgggtaaac tggttggtgt cgagcgggtc catcgggctc
     5701 tggttcttga gctgcgtcgt cagcaggccg aggaatgcat cgaaattctg agcgatcgat
     5761 gcccgcgtgc cgcctgccgc ggtgccggac gtggcggagg cggtgttggt cagggcggtt
     5821 gaaatggtgc tcatgaggcg tccttacagc atgatgtcga tggccgaacg cagcgccagc
     5881 ctgcgggccg taccagcacc gggctgggac tggtcctcgg cgctgcggtt catgggatcg
     5941 tccgccagct ccgtgtgggc gtggcggtag gcggcatggt gctgaccgtc gccgccctgc
     6001 ctgaggctga cctggatgcc ggtgccgtcg ctgcgcaggc ctgtttcggc gaaggcctgc
     6061 tgcagggcgt gggcctcccg gcgcagtgcc gcaagcgctt ccggacgctc gaccaccagg
     6121 tgcgcgcgca cctggccgtc gcggtcgatg tcgagacgga cgtcgacccg gccgaggtct
     6181 tccggctcca gcctgatctc gaaacgccgg gcgccgtcac gcgccatgcc ggcgatcagc
     6241 accggtacct gc
//
LOCUS       DAOMXN010000155         6229 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_6639_length_6229_cov_12.023486, whole genome shotgun sequence.
ACCESSION   DAOMXN010000155 DAOMXN010000000
VERSION     DAOMXN010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 6229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6229
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6639_length_6229_cov_12.023486"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="PK812_10455"
     CDS             complement(<1..264)
                     /locus_tag="PK812_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRJ70018.1"
                     /translation="MPAACRVVRLKLTDFRTYAAADLTIGAQLVALSGENGAGKTNVL
                     EALSLLAQGRGLRRAELADMARRGGAGGFAVSAEIEGACGGAVL"
     gene            complement(302..1420)
                     /gene="dnaN"
                     /locus_tag="PK812_10460"
     CDS             complement(302..1420)
                     /gene="dnaN"
                     /locus_tag="PK812_10460"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRJ70019.1"
                     /translation="MKVTVEQAALLKSLGHVHRVVERRNTIPILSNVLLRAEAGKLWL
                     KATDLDIEVTESLAADAAQGGSTTVPAHVLYDIVRKLPDGAQVSLEQAGDASQLTVRS
                     GRSRFTLQALPDSDFPDLAAGTLSHRFEVAATELRRLLEKTQFAISTEETRYYLNGIY
                     LHAVEGEAGAILRAVATDGHRLAQSDTPAPKGASGMPGVIVPRKTVAEIQKLLAEAEG
                     DITVELSTAKIRFSVGSVVFTSKLIDGTFPDYARVIPTRNDKRLTVELDEFARSVDRV
                     STISSERGRAVKLALADGKLVLSVNNPDAGQAVEELSVDYESGPLDIGFNARYLLDIT
                     AQLDGDTALFKLADPGSPTLIQDRDGAPNLYVLMPMRV"
     gene            complement(1542..3044)
                     /gene="dnaA"
                     /locus_tag="PK812_10465"
     CDS             complement(1542..3044)
                     /gene="dnaA"
                     /locus_tag="PK812_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HRJ70020.1"
                     /translation="MFNASSHQEKETGAAERDGAQDRLAPREEARVWERVRRRLRAEL
                     GDDIFTSWFARLELVSVSDGLAQLSVPTRFLRSWLVGHYADRIEVLYREENPAVQRIA
                     IALREAGARTAPRKAAGAPHQAGAPSGPAAATATPASLSLTPAVATRSDDSFESSPLD
                     RRLTFASFHVGRSNMMAHAAAMQVVQAQPGQGPVYNPLFVHSAVGMGKTHLLQATAQA
                     VSGTDRRVIYLTAEKFMYGFVASLRAQTALSFKENLRAIDVLVIDDAQFLNGKQTQQE
                     FCHTLNALIDAGKQVIVAADRNPADLDMLDERIQSRLAGGLVVEIGPLDEALRLRVLE
                     SRIQTAKAHLPAFHIGSDVLAHVARCVTANGRDLEGAVNRLLAHATLGGGPLDLETAE
                     VAIKDLIRTQEPKRVKIEDIQRLVANHYSVSRADILSSRRTATVVRPRQIAMYLSKTL
                     TLRSLPEIGRRFGGRDHTTVLHAVRKIDGLIGTDISLAGEIDHLRRMLQE"
     gene            complement(3819..4085)
                     /gene="rpsT"
                     /locus_tag="PK812_10470"
     CDS             complement(3819..4085)
                     /gene="rpsT"
                     /locus_tag="PK812_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HRJ70021.1"
                     /translation="MANTKSAKKAARQAVARTEVNKSRRSRMRTAVRKVEEAIAAGTA
                     DVAATELRNAEPELMRAARTGVIHKNTASRKVSRLTKAIKALQA"
     gene            complement(4249..5055)
                     /locus_tag="PK812_10475"
     CDS             complement(4249..5055)
                     /locus_tag="PK812_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HRJ70022.1"
                     /translation="MPDLMSLIGVSFSTLMALMAVSFCAAIVDAIAGGGGLLNVPSLM
                     IAGLDPVSAVATNKVQGSFGVASSTLAYARAGLIRFREMAFLFLCSFVGAGAGAVAAQ
                     TLPIGALRTWIPLVLIGLALFVATAPKLTDADSHARLRPAVFAFLFAAPIGFYDGMFG
                     PAGGTFFFIALVTMLGQGVLKAVAHTKLLNLASNLGALCLFLFSGKIYWAVGLCLGVA
                     AFAGAQIGARAALSRGARLVKPMVVAVAVLMAIRLLLDPAHPVGQWLRGG"
     gene            complement(5128..5913)
                     /locus_tag="PK812_10480"
     CDS             complement(5128..5913)
                     /locus_tag="PK812_10480"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HRJ70023.1"
                     /translation="MGLKASYDNIIAEVHGKVGVIQLNRPQALNALNSFLIEDLNHAL
                     EVFETDPNIGCTVLTGSDKAFAAGADIKQMVSKTYMEAYLDDFIGRWDYLSRTRKPVI
                     AAVAGFALGGGCEIAMMCDFIIAADTARFGQPEIKLGVMPGSGGTQRLTRFIGKAKAM
                     DLCLTGRMMDAAEAERCGLVSRIVPADQLVAEALKAAETIASLSLPAVMMTKETVNRA
                     YETTLAEGIRFERRVFHSMFSLEDQKEGMTAFAEKRKPNFKNR"
     gene            complement(5974..>6229)
                     /locus_tag="PK812_10485"
     CDS             complement(5974..>6229)
                     /locus_tag="PK812_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010913344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HRJ70024.1"
                     /translation="GARRQAARERLATAIREVLAEAIAAGGSTLKDYAHTDGSLGYFQ
                     HRFRVYDREGEPCPTPGCRGVILRTVQSGRSTYHCKVCQR"
BASE COUNT         1131 a         2038 c         2009 g         1051 t
ORIGIN      
        1 aagcacggcg ccgccgcacg ccccttcgat ctcggccgaa acggcgaatc cgccagctcc
       61 cccgcgccgc gccatgtccg caagctcggc acgacgcagg ccccgtccct gggcgagcag
      121 cgagagcgcc tccagcacgt tcgtcttgcc cgcgccgttt tctccggaga gcgcaaccaa
      181 ctgcgcgccg atcgtcaaat cggctgcggc ataggtgcgg aaatcggtca gtttcagccg
      241 gacaacgcgg caggcagcag gcatgggctg gcctagcgca tgggcaaagg cgtgtcgagc
      301 gtcacacccg catcggcatc aggacgtaga gattcggcgc gccgtcccgg tcctggatca
      361 gcgtcggcga ccccggatcg gccagcttga acagcgcggt atcgccatca agctgcgccg
      421 tgatgtcaag caggtagcgg gcgttgaagc cgatatcgag gggacctgac tcgtaatcga
      481 ccgacagttc ctcgaccgcc tggcccgcat ccgggttgtt gacggacagc acgagcttgc
      541 catccgccag cgccagcttc accgcgcggc cgcgctcgga cgagatggtg gagacgcgat
      601 cgacggaacg ggcgaattca tcgagttcaa cggtcagacg cttgtcgttg cgcgtcggga
      661 tgacgcgggc atagtccggg aaggtgccgt caatcagctt ggaggtgaag acgaccgacc
      721 ccaccgaaaa gcggatcttt gccgtcgaca gttcgaccgt gatgtcgccc tcggcttccg
      781 ccagcagctt ctggatctcg gcgaccgtct tgcgcggcac gatcaccccc ggcatgccgg
      841 aagcgccctt cggcgccggc gtatcggact gcgccaggcg gtgtccatcg gtcgccacgg
      901 cgcgcaggat ggcaccggct tcgccctcga cggcatgcag gtagatgccg ttgagatagt
      961 agcgcgtctc ctcggtcgag attgcgaatt gggtcttctc caaaaggcgg cgcagctcgg
     1021 tggccgccac ctcgaagcgg tgcgacagcg ttcccgcagc gaggtccggg aaatcgctgt
     1081 ccggcagcgc ctgcagggtg aagcgggatc ggcccgaacg caccgtcagc tggctggcgt
     1141 cccccgcctg ttccagcgac acctgcgcgc cgtccggcag cttgcgcacg atgtcataga
     1201 gcacatgggc gggaaccgtg gtcgagccgc cctgggccgc atcggccgcc agcgattccg
     1261 tcacttcgat atcgaggtcg gtcgccttga gccagagttt cccggcctcg gcgcgcagca
     1321 gcacgttcga caggatcggg atcgtattgc gcctctccac cacacggtgc acgtggccca
     1381 gcgacttcag aagcgcggcc tgctcgacag taaccttcat accccaccca cccggcgcaa
     1441 aatggaccgc acgatggcgc gagtttccgg ttcgcgcaag ccgcgcaacg gtccgggcgg
     1501 agaaatccac ccctttttgc cggaccgatc gccggaccgc cctattcctg cagcatgcgc
     1561 ctgaggtggt cgatttcacc ggcaagcgag atatccgtgc cgatcagccc gtcaatcttg
     1621 cgcacggcgt gcagcacggt cgtgtgatct cggccgccga agcgccgccc gatctccggc
     1681 agggagcgca aggtcagagt cttcgacaga tacattgcga tctggcgcgg tcgcaccacg
     1741 gtcgcggtgc gccgcgacga gaggatgtcg gcgcggctca cgctgtagtg gttggcaaca
     1801 aggcgctgga tgtcctcgat cttcacgcgc ttgggctcct gcgtgcggat cagatccttg
     1861 atcgcgacct cggctgtctc gaggtccagc ggtccgccgc ccagcgtcgc gtgggcgaga
     1921 aggcggttga ccgcgccttc aagatcgcgc ccgttcgcgg tcacgcaccg cgcgacatgg
     1981 gccagcacat ccgagccgat atgaaaggcc ggcaggtgcg ccttggcagt ctgaatgcgg
     2041 ctctccagca cgcgcagccg caaggcttcg tccagcgggc cgatttccac gaccaacccg
     2101 cccgccagcc tcgactggat gcgttcgtcc agcatgtcga ggtccgccgg attgcggtcc
     2161 gcagcgacga tcacctgctt tccggcgtcg atcagcgcgt tcagcgtatg gcagaactcc
     2221 tgttgcgtct gcttgccgtt gagaaattgc gcgtcgtcga tcaccagaac gtcaatggcg
     2281 cgcaggttct ccttgaagga cagggccgtc tgcgcacgca gcgaagcgac gaagccgtac
     2341 atgaacttct cggcggtgag atagattacc cgcctgtcgg tcccggaaac ggcctgcgcg
     2401 gtcgcctgca gcagatgggt cttgcccatc cccaccgcag aatgcacgaa cagcggattg
     2461 tagaccggtc cctgccccgg ctgcgcctga accacctgca tggccgcggc atgcgccatc
     2521 atgttggagc ggccgacatg aaagctggcg aaagtcaggc gacggtcgag cggcgagctt
     2581 tcgaaactat cgtccgagcg tgtggcgaca gcaggcgtga gggataggct ggccggcgta
     2641 gccgtggcgg cggcaggtcc tgacggggcg ccggcctgat gcggagcacc agccgccttg
     2701 cgcggcgcgg tccttgcacc ggcttcgcgc aaggcaatgg cgatgcgttg aaccgccggg
     2761 ttctcctccc ggtagagcac ctcgatgcgg tcggcgtagt gaccgaccag ccaactgcgc
     2821 aggaagcgtg ttggaaccga aagctgggcc agaccgtcgc tcacggacac cagctcgagg
     2881 cgcgcgaacc agctggtgaa gatgtcgtca cccagttcag cacgcaggcg ccgacgcaca
     2941 cgttcccaga ctcttgcctc ttccctcggg gcgaggcgat cctgcgcccc gtcccgttcc
     3001 gctgcgcccg tttctttttc ttggtggcta gatgcgttga acatggtcag gctcgagcaa
     3061 tgctttcggg ttccgggaaa gcgtgctttc caggtcgacg tttcggatgt tcggtgtttg
     3121 cagttccctc agggagaacc acgtcgggtc cggcgggctt gctacgcact caggatcgcc
     3181 ttccagaccc aaggcactga tgcgacggcc cgccagccct cgtcgcgtca gacgacacaa
     3241 aagtccgccc gctccgccgc cgcggtccgc acgcctggat gagcggtcgg atgcaacggc
     3301 atcgccaggc aaaagaacat cctctcccga agcgggagtt cgtctcctac cgatttcaga
     3361 aagcccggcg gcgccggtgt tgagggagtg ttagccagcg atccgagggc ctgcaacatc
     3421 cgtttttccg cgaccgggca gctcggtcgc gtcattgccg acctgtcgcc ttcggcaaga
     3481 tggggggagc gccggcaagc tgttgaagct cttgcgattc accttgggtg ccgtttacgg
     3541 atttttaatg tttcgatatc gaaacgattt gcctgaccgg ccggaccgac ggaaccggcc
     3601 acgcccacga cctcggacgg aaatcgctct tggcgcttat cctattggaa atacagcaca
     3661 aattattatc gatgggcgcg acaggggcgg ctgttcacac aatgcctctg tcaagtgaaa
     3721 aggcgttgcg gggcgtgaaa aaatgcggcg acacactgtc gcaaatgcaa aaagggcccg
     3781 gcagtgagcc aggccctcaa tccaaaccgg aagccgtttc aggcctggag ggccttgatc
     3841 gccttggtca gacgggaaac cttgcgcgag gcggtgttct tgtgaatgac gccggtgcgc
     3901 gcagcgcgca tcaactcagg ctcggcgttg cgcagctccg tcgccgcgac atcggccgtg
     3961 ccggccgcga ttgcttcctc gaccttgcgc accgccgtgc gcatgcggct gcgacgggac
     4021 ttgttgacct cagtgcgcgc aacggcctga cgcgcagcct tcttggccga tttcgtattc
     4081 gccatcgttc tcaatctccg gaagcgatgc tccggcctac gggccgctcc gcatcgatcc
     4141 aatgggcgtg aaaattagtg cgcgccgacg aggccgccgg cgcttgcgcg gctcattggc
     4201 acgatcaccg cgatccgtca acgggtagcg gggcgccacc tgaaggaatc agccgccgcg
     4261 cagccactgg ccaaccggat gtgccggatc gagcagcaac cggatcgcca tcaggaccgc
     4321 caccgccaca accatcggct tgaccagtcg cgcacccctg ctcagggcgg cgcgcgcacc
     4381 gatctgagcg cccgcgaaag cggcaacccc gaggcacagc ccgacggccc agtatatctt
     4441 gccggaaaac aggaacaggc agagcgcgcc cagattgctc gccagattta gcaatttggt
     4501 atgcgccacg gccttcagca cgccctgtcc cagcatcgtc accagagcga tgaaaaagaa
     4561 ggtaccgccg gctggaccga acatgccgtc ataaaagccg atcggcgcgg cgaacagaaa
     4621 agcgaatacc gcggggcgca ggcgtgcgtg ggaatcggca tcggtcagct tcggagcggt
     4681 cgccacgaac agcgcgaggc caatcagcac cagcggtatc caggtccgga gcgccccgat
     4741 cggcagggtc tgcgccgcga ccgcgccggc gcccgcgccc acgaacgagc acaggaacag
     4801 gaatgccatc tcccggaacc ggatcagccc ggcgcgcgca taggccaaag tcgaggaggc
     4861 aacgccaaag gaaccctgaa ccttgttggt ggcgacagcc gagactggat ccagtccggc
     4921 gatcatcagc gagggcacgt tgagaagacc gccgccgccg gcgatggcat caacgatggc
     4981 ggcgcagaag ctgaccgcca tcaaggccat gagcgtcgag aatgaaacgc cgatcagcga
     5041 catcaggtcg ggcatggcgt ttcttaccgt tcgcttcctg gcggtcctgg acccggtctg
     5101 ccgaaacggc gggccggagg gcgccggtca gcggttcttg aagttgggtt tgcgcttctc
     5161 tgcgaaggcc gtcatgcctt ccttctggtc ttccagcgag aacatggagt gaaacacgcg
     5221 ccgctcgaag cgaatgccct cggccagcgt ggtctcgtag gctcgattga ccgtttcctt
     5281 ggtcatcatc accgccggaa gcgacaggga ggctatcgtc tcggccgcct tgagcgcctc
     5341 ggcgacgagc tgatcggcag gcacgatgcg cgagaccagg ccgcaacgct cggcttcggc
     5401 agcatccatc atgcgtccgg tgaggcagag gtccatggcc ttggccttgc cgatgaagcg
     5461 ggtgaggcgc tgcgtgccgc cagaccccgg catgacgcca agcttgatct cgggctggcc
     5521 gaacctggcc gtgtcggcgg cgatgatgaa atcgcacatc atggcgatct cgcagcctcc
     5581 gccgagcgcg aacccggcca ctgcggcgat caccggcttg cgggtgcgcg acagataatc
     5641 ccagcggccg atgaaatcgt cgagataggc ctccatgtag gtctttgaca ccatctgctt
     5701 gatgtcggcg ccggcggcga aggccttgtc gctgcccgtg agcacggtgc agccgatatt
     5761 cggatcggtc tcgaagactt ccagcgcatg gttgaggtcc tcgatcagga aggaattgag
     5821 cgcattcaac gcctgcgggc ggttcagctg gatcacgccg accttgccgt gcacttccgc
     5881 gatgatgttg tcgtagctgg ctttcaggcc catggcgcgc tcctcgatcc gaatgcgctg
     5941 agggctacag gaggcgggag cgccccgcaa gggtcagcgt tggcaaacct tgcaatggta
     6001 cgtcgagcgc ccggactgca cggtacgcag gatgacgccc cgacagcctg gcgtcgggca
     6061 gggctcgccc tcccggtcgt aaacacgaaa acggtgctgg aaatagccaa gggaaccgtc
     6121 ggtatgcgca tagtccttca atgtcgatcc gccagcggcg atggcctcgg ccaggacctc
     6181 gcggatggcc gtcgccagcc tctcgcgcgc cgcctgccgc cgggcaccg
//
LOCUS       DAOMXN010000156         6168 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_6738_length_6168_cov_12.208572, whole genome shotgun sequence.
ACCESSION   DAOMXN010000156 DAOMXN010000000
VERSION     DAOMXN010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 6168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6168
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6738_length_6168_cov_12.208572"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..2151
                     /locus_tag="PK812_10490"
     CDS             169..2151
                     /locus_tag="PK812_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70025.1"
                     /translation="MLDQLYVKKIRTQKIRVAVVLLSFVGVMVWQKHFIITGFSHNIP
                     LNGAIFLIFFFAAGLAIKDLYGLRNEQLAFEALREKYQDIQQNAEKSAEDPFWKHYRC
                     LEKGTVYQKPDLLGHVYELVFEELLRTRHMRISVSTLQNLTAALSAAAAHRRSLLIYI
                     AGLEVFLGLIGTFIGLMEMVESVGGIIGGVAGADASSSDAMKKLLTDLEAPLKGMSTG
                     FSASLFGLGTSMCIGLIGRFVASATDAMIEAFEGWLAGIAQIENEKKDAAGMVAEDAT
                     FMAAPGVAAIISALRSTNHTFEKTADAVLKLADRRKEENEILMATAGQIERLTSEQDG
                     MRRAITSLGGIRDEINGLRQLDYAQANRVVEGFAKIESAIEENRAVTQKGFEEVSAQE
                     SESMKQSRMSAEQVSRKIDEIVAQGARNHLDAADRLESLAQLQASLSRLLQTSQTQAG
                     SKMDDLMSSLERLHAVTTDQQQQLSSTRGELSSGIRELQLRPDVSPQVQQMSVTMDST
                     MVKGLGEIANVLNTALGVLTQSMREIAVKQQDANTVITAMSETKELANEIRMLGKSID
                     GGLAHGFAEMSKSFEAVFMSYSELINRTGARDDVARSVVPANATARVEVQLSEAAKGA
                     PPGPAKPANAVHENLAQVDKFYASAQSRLSDLLKSA"
     gene            2207..2620
                     /locus_tag="PK812_10495"
     CDS             2207..2620
                     /locus_tag="PK812_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70026.1"
                     /translation="MALSLPSLGGIAGSIGARIPFIKRNRTGSHMRAHQRIQCCLMGE
                     VSVVERGFKIEGLVIEVSRGGMRFREASHFVMNRTGVDVTILVSGGEYPGRIMNSSTT
                     GYGIKLDSLITEEEMERLIASGAPKGFDATGEAET"
     gene            2713..3498
                     /locus_tag="PK812_10500"
     CDS             2713..3498
                     /locus_tag="PK812_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014285152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRJ70027.1"
                     /translation="MKLDTSIAAIVTGGASGLGEASARRLASHGVKVALFDLNEERGK
                     KVAAEIGGIFCKTDVTSEESVVASLAAARQAHGQERIAVNCAGIAIAKRTLGRNRETG
                     DLVLHDLASFSKVIQINLVGTFNVLSKSSAGMAKAAPLTDDGLRGVIVMTASVAAEDG
                     QIGQVAYAASKGGVASLTLPAARDLAQYGVRVVTILPGLFHTPMFDTLPEEARKALGA
                     SVPFPSRLGRPDEYAQLVQQICENDMLNGGTIRLDGSLRMAPK"
     gene            complement(3502..4032)
                     /gene="thpR"
                     /locus_tag="PK812_10505"
     CDS             complement(3502..4032)
                     /gene="thpR"
                     /locus_tag="PK812_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HRJ70028.1"
                     /translation="MPRLFTGIEVPPDIAGTLAALRGGLPGARWSEPSDYHITLRFIG
                     DVDRHTASEIAESLDELQVDPPEIRLTGLDTFGGARPHVVFAAIDNSRPLNELQGDHE
                     RLIRRAGLAPERRKYTPHVTLARLRSSSVLDVADFLSTRGGFRAMSFRPRRFVLYSAR
                     EKVGGGPYVVEAAYPF"
     gene            complement(4118..4678)
                     /locus_tag="PK812_10510"
     CDS             complement(4118..4678)
                     /locus_tag="PK812_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70029.1"
                     /translation="MLDLWKDPWPLDVKQCPCDIHFTDWLASAGVRNATIFHFGTGDH
                     HHVGKTEAMREAGNSVLGITATKPEYDSYIDLVIRHPLVGKRYKVLFTDIYQLAPELM
                     PEFDVVTLFHLCEFWSETNAPYARMNDSGVLEAMAGKVKRGGKLLFYTGSFAASTASK
                     LVDANIARLGFGPAERHETLLVHTKR"
     gene            complement(4701..5297)
                     /locus_tag="PK812_10515"
     CDS             complement(4701..5297)
                     /locus_tag="PK812_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012318016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HRJ70030.1"
                     /translation="MTANRSATAATPLKLVALGDSLTAGYLLPADAAFPVVLEKALRA
                     RGHVIEITNAGVSGDTTAGGLARLDWSVPDGTRGVILELGANDALRGLDPKQAEQNLE
                     AMIAGLKGRGIRVLLAGMLAPRNNGPDYAAAFDAIYPRLAARHGLRLYPFFLDGVMGE
                     AGMTLPDQMHPSRKGVETIVARILPTVESFIADISAGR"
     gene            5445..6149
                     /locus_tag="PK812_10520"
     CDS             5445..6149
                     /locus_tag="PK812_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ70031.1"
                     /translation="MSAGTDPAILLKDVHLSLGSGASRVHILRGVSLSIGRGEAVGLV
                     GPSGSGKSTLLMVAAGLERQDSGQVAIAGQDLAALDEDGLARFRGRSIGIVFQAFHLI
                     PTMTALENVAIPLELAGRADAHRRARDELEAVGLGARIHHYPPQLSGGEQQRVALARA
                     LAPSPAILVADEPTGNLDEGTGRQIIDLMFDLKRERGATLVLVTHDGALAARCDRRIR
                     LRSGEIIEDRERVAAE"
BASE COUNT         1082 a         2016 c         2076 g          994 t
ORIGIN      
        1 cccccgccac tccagcctga ctacggcggc cgggcggctc cggccagcgc ctcccgcgaa
       61 agtgcaggcg acctgcgaaa ttcgcgacgt cattaaccgt ttgttgcccg acttggcata
      121 atttgagcag cacccgggct gttgtggcgt tgccggggga gatgcgtgat gctcgatcag
      181 ttgtatgtga agaagatccg gacgcagaag atccgcgtcg ccgtggtcct gctgtcgttc
      241 gtcggcgtga tggtctggca gaagcacttc atcataacgg gcttcagcca caatattccg
      301 ctcaacggcg cgatcttcct catcttcttc ttcgccgccg gcctcgccat caaggacctc
      361 tacggcctca ggaacgaaca gctcgccttc gaagcgctgc gggaaaaata ccaggacatc
      421 cagcagaacg cggagaagtc ggccgaggac ccgttctgga agcactaccg ctgcctggaa
      481 aagggaacgg tgtatcagaa gccggatctg ctcgggcatg tctacgagct ggtgttcgag
      541 gaactgctgc gcacccggca catgcgcatc tccgtctcca cgctgcagaa cctgacggcg
      601 gcgctgagcg cggcggcggc acatcggcgt tcgctgctga tctacatcgc cggactggaa
      661 gtctttcttg gtctgatcgg caccttcatc ggactgatgg aaatggtgga atcggtcggt
      721 ggcatcatcg gcggcgtcgc cggcgccgac gcctcgtcgt ccgatgccat gaaaaagctg
      781 ctgaccgacc tcgaagcgcc gctgaagggc atgtccaccg gcttctcggc ctcgctgttc
      841 ggcctcggca cctccatgtg catcggtctg atcggacggt tcgttgcctc cgcgaccgac
      901 gcgatgatcg aggcgttcga gggctggctg gcgggcatcg cgcagatcga gaacgagaag
      961 aaggacgccg ccggcatggt ggcggaggac gccaccttca tggccgcgcc cggcgtggcc
     1021 gccatcattt cggcgctgcg ctcgaccaac cacacgttcg agaagacggc ggatgccgtg
     1081 ctcaagctgg cggaccggcg caaggaggag aacgagatcc tcatggccac cgccggccag
     1141 atcgagcgcc tgacctccga gcaggacggc atgcgccgcg ccatcacctc gctgggcggc
     1201 atccgcgacg agatcaacgg cctgcgccag cttgactatg cccaggcgaa ccgcgtggtc
     1261 gagggatttg ccaagatcga atcggccatc gaggagaacc gcgccgtcac gcagaagggc
     1321 ttcgaggagg tctccgccca ggaatccgaa tcgatgaagc agtcgcgcat gtcggcggag
     1381 caggtgtcgc gcaagatcga cgagatcgtg gcgcagggcg cccgcaacca cctcgacgcc
     1441 gccgaccgcc tcgaaagcct tgcccagctc caggccagcc tgtcgcgcct gctgcagacg
     1501 tcgcagacgc aggccggctc caagatggac gatctgatga gttcgctgga gcgcctgcac
     1561 gcggtcacca ccgaccagca gcagcagctt tccagcacgc gcggcgaact gtcctccggt
     1621 atccgggaac tgcagctgcg cccggacgtc tcgccgcagg tgcagcagat gtcggtcacc
     1681 atggattcca ccatggtcaa gggtctcggc gagatcgcca acgtgctcaa cacggcgctt
     1741 ggcgtcctga cccagagcat gcgcgagatc gcggtgaaac agcaggatgc aaacaccgtc
     1801 atcaccgcga tgagcgagac gaaggaactg gccaacgaaa tccgcatgct gggcaagtcc
     1861 atcgacggcg gactcgcgca cggcttcgcg gaaatgtcca agtctttcga ggcggtgttc
     1921 atgtcctact cggaactcat caaccgcacc ggagcgcgcg atgacgtggc gcgttcggtc
     1981 gttccggcca atgccacggc ccgcgtcgag gtgcagttga gcgaggccgc gaagggcgcc
     2041 cccccgggcc cggccaagcc tgccaacgcc gtgcacgaaa acctggcgca ggtggacaag
     2101 ttctacgcct cggcgcagag ccgcctgtcc gaccttctga agagtgcctg atcatggcgc
     2161 cgtcgggggc atcccccggc gcccgcgtcc gaaccggaga acagcgatgg ccctgtccct
     2221 tccctctctc ggcggcatcg ccggcagcat cggtgcccgc attccgttca tcaagcggaa
     2281 ccggacgggc agccacatgc gcgcgcacca gcgcatccag tgctgcctga tgggcgaagt
     2341 ctccgtggtc gagcgcggct tcaagatcga ggggctggtg atcgaggtgt cgcgcggcgg
     2401 catgcgcttc cgagaggcgt cgcatttcgt gatgaaccgg accggtgtcg atgtcacgat
     2461 cctggtctcg ggcggcgaat acccgggcag gatcatgaat tcgagcacga ccggctacgg
     2521 catcaagctc gacagcctga tcaccgagga agagatggag cggctgatcg cctcgggagc
     2581 gccgaagggc ttcgacgcca ccggcgaagc cgagacctga tccggccggg ccggcgggcg
     2641 cgccaacagc gcccttgcgt ccgctgacgt ctaagtgcat tccctgtgcc caccagagcc
     2701 aaggggaacg gcatgaaact cgacacatcc attgcggcaa tcgtcacggg cggggcttcc
     2761 ggcctcggtg aagcctcggc gcgccggttg gccagccacg gcgtgaaagt ggcgctgttc
     2821 gacctcaacg aggagcgcgg caagaaggtc gctgcggaga tcggcggcat cttctgcaag
     2881 accgacgtga ccagcgagga gtcggtcgtg gcatcgctgg ccgcggcgcg tcaggcgcat
     2941 ggccaggagc gcattgcggt caactgcgcc ggcatcgcca tcgccaagcg cacgctcgga
     3001 cgcaatcgcg agaccggcga tctggtgctg cacgatctcg ccagcttctc gaaggtcatc
     3061 cagatcaacc tggtcggcac gttcaacgtg ctgtccaagt cgagcgccgg catggcgaag
     3121 gccgcgccgc tgacggacga cgggttgcgc ggtgtcatcg tcatgaccgc gtccgtcgcc
     3181 gcggaggatg ggcagatcgg ccaggtcgcc tacgcggcct ccaagggcgg cgtcgcctcg
     3241 ctgactttgc cggccgcgcg tgacctcgcc cagtacggcg tgcgcgtggt caccatcctg
     3301 ccggggctgt tccatacccc gatgttcgac acgctgccgg aagaggcgcg aaaggcgctc
     3361 ggcgcctcgg tgcccttccc ctcgcgcctt ggccgtccgg acgaatacgc ccagctcgtg
     3421 cagcagatct gcgagaacga catgctcaac ggcggcacca tccgcctcga cggttcgctc
     3481 cgcatggcgc cgaaataggc cctagaacgg gtaggcggcc tccaccacat aggggccgcc
     3541 gccgactttc tcgcgcgccg aatacaacac gaagcgccgc ggccggaagc tcatggcgcg
     3601 gaagcccccg cgcgtggaca gaaagtccgc cacatcgagc acggaggacg agcgcagccg
     3661 ggcgagcgtg acatgcggcg tgtacttgcg tcgctccggc gcgagcccgg cgcggcggat
     3721 cagccgctcg tggtcgccct gcaactcgtt cagcggccgg ctgttgtcga tggcggcgaa
     3781 gacgacatgc gggcgcgcgc cgccgaaagt gtcgagaccg gtcaggcgga tctccggcgg
     3841 atcaacctgc aattcgtcaa gggattcggc gatctcgctg gcggtgtgcc ggtcgacgtc
     3901 tccgatgaag cgcagcgtga tgtggtagtc agacggttcg gaccagcggg cgcccggaag
     3961 gccgccgcgc agtgcggcca gcgttccggc aatatccggc ggcacctcga tacccgtgaa
     4021 cagacgcggc atcgcaaccc ccacgcgaat cacagtctgg gcaaaaccta gcaccgatgc
     4081 gcgacagcca ggcaacgggt ctgtggaagg cccatcctca gcgcttcgtg tggaccagca
     4141 gcgtctcgtg ccgttcggcc gggccgaatc ccagccgcgc gatgttggcg tctacaagtt
     4201 tcgaggccgt cgaggccgcg aaggaaccgg tgtagaacag caacttgccg ccgcgcttga
     4261 ccttgccggc cattgcctcc agcacaccgg aatcgttcat gcgggcgtaa ggagcattgg
     4321 tctcgctcca gaattcgcag aggtggaaca gcgtgaccac gtcgaactcc ggcatgagct
     4381 cgggcgcgag ctggtagatg tcggtgaaca gcaccttgta gcgcttgccg acgagggggt
     4441 ggcggatcac cagatcgata tagctgtcgt actcgggctt ggtggccgtg atgccgagca
     4501 cggaattgcc ggcttcccgc atcgcctcgg tcttgccgac gtggtggtgg tcgccggtgc
     4561 cgaaatggaa gatcgtggcg ttccgcactc cggcagacgc gagccagtca gtgaaatgga
     4621 tgtcgcaggg acattgcttg acgtccagcg gccaggggtc cttccagaga tcaagcatgg
     4681 cattctcctg atggtgccgg tcagcggccg gccgatatgt cggcgatgaa ggattcgacg
     4741 gtgggcagga tgcgggcgac gatcgtctcg acgcccttgc ggctggggtg catctgatcc
     4801 ggcagggtca tgccggcctc gcccatgacc ccatccagaa agaacggata caggcgcaga
     4861 ccgtgccttg ccgcgagccg cgggtagatc gcgtcgaagg cggcggcgta gtccgggcca
     4921 ttgttgcgcg gcgccagcat gccggcgagc aggacgcgga tgccccgccc tttcagcccc
     4981 gcgatcatgg cctcgaggtt ctgctcggcc tgtttcggat ccagaccgcg cagcgcatcg
     5041 ttggcgccga gttcgagaat gacgccccgc gtcccgtcag gaacgctcca gtcgagacgc
     5101 gccagtccgc cggccgtggt gtcgccggaa accccggcat tggtgatctc gatgacgtgc
     5161 cccctggcgc gcagcgcctt ttccagcacg acggggaagg ccgcgtccgc aggcagcaga
     5221 tagccggcgg tcaagctgtc gccgagggcg accagcttca gcggcgtggc ggctgtcgcg
     5281 gaacggttcg ccgtcatggc gccgaatccg gccagtagga ccggtgcggc gagcatggaa
     5341 cggcgcgaga acggacgttt gtttgcatgc atggcttggc cttaagccaa ttcgtaccta
     5401 tctgccagtg agccgtcgat gaacagccct gtcagggaga gacaatgtcc gctggaaccg
     5461 atcctgcgat cctgctgaag gatgtccatc tcagtctcgg gtccggtgcc tcgcgcgtgc
     5521 acatcctgcg cggcgtgtcg ctgtcgatcg gccggggtga agcggtgggc ctggtcgggc
     5581 catcgggctc cggcaagtcg acgctgctga tggtcgccgc cggtctggaa cggcaggaca
     5641 gcggtcaggt cgccatcgcc gggcaggatc tggccgccct ggacgaggac gggctcgccc
     5701 ggttccgcgg tcggtccatc ggcatcgtct tccaggcctt ccacctgatc cccaccatga
     5761 cggcgctcga gaacgtcgcc attccgctcg aactcgcggg gcgggcggat gcgcatcgcc
     5821 gcgcgcgcga cgaactcgag gcggtcggtc tcggcgcccg catccatcac tatccgcctc
     5881 agctgtcagg cggcgagcag cagcgcgtgg cgctggcgcg ggcgctggcg ccatcgcctg
     5941 cgatcctcgt cgcggacgag cccaccggca atctcgatga gggaaccgga cggcagatca
     6001 tcgatctgat gttcgatctc aagcgggaac gcggcgccac cctcgtgctg gtgacgcatg
     6061 acggggcgct cgccgcgcgt tgcgaccggc gaatccggct caggtcgggc gagatcatcg
     6121 aggaccgcga gcgggtggcg gcggaatgag cgcgccggcc cccgcctt
//
LOCUS       DAOMXN010000157         6087 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_6852_length_6087_cov_10.306366, whole genome shotgun sequence.
ACCESSION   DAOMXN010000157 DAOMXN010000000
VERSION     DAOMXN010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 6087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6087
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6852_length_6087_cov_10.306366"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1334)
                     /locus_tag="PK812_10525"
     CDS             complement(<1..1334)
                     /locus_tag="PK812_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break repair protein AddB"
                     /protein_id="HRJ70032.1"
                     /translation="MSGSAAPRSPRVISIPAGAPFLETLVELLLRGTLFGRSDPLRPD
                     ELADALLFLPTRRAAEVFASIIAARAPQGALMLPRIVLLGDPEPDSAAGSGLGGPAGL
                     PGLPPAIDPLVRQLKLFELVDAWRRAIETGTRPHGDGEPFFVARSRADAFALAGDLAG
                     LIDETIIEAVPLERLGQGMPPTFDPSRHDAYWSLTAKFLEIAAQQWPVILAELGQSDA
                     EHRTRSLLLEQAQRLAQSGTGRPVIVAGSTGSVSATAELMRCVAGLTMGAVVLPGLDT
                     TLDAAAWDAIGAEDAALATRFAHPQASMKRALARIGIGRADVVLHGHSRIATRPQLAS
                     EVFRPAEASDAWSALRQDKVPEGMALVEAADGGEEALAIALILREALETPGRTIALAT
                     PDRMLARAVQAELERWNVRVADSAGLRMIESPAGILARLALLAARPDAGGTAL"
     gene            complement(1331..2053)
                     /locus_tag="PK812_10530"
     CDS             complement(1331..2053)
                     /locus_tag="PK812_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012321462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRJ70033.1"
                     /translation="MASPQINTAMILAAGLGKRMRPLTDLRPKPMIEVAGKALIDYNF
                     DRLEEAGVTRAVVNVHYLADMLEAHLRARHSPDIAISDERAAILDSGGGVKKALALLG
                     EGPFFSLNADTIWMDGPRSNLVRMAETFDPATMDILLLLAPTVSTVGWGNRGDLAMEQ
                     DGRLRWPRPGEVAPFAYCGAAILDSRLFSDTPDVFSLLLLFNRAMAAGRLFGLRLDGR
                     FMHVGTPEAIVEAEAAIIGSSR"
     gene            complement(2061..>2621)
                     /locus_tag="PK812_10535"
     CDS             complement(2061..>2621)
                     /locus_tag="PK812_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007567580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HRJ70034.1"
                     /translation="LMPDWYLPHKGHHALSATSRIAFIDLWSALLQPLLAGPGTWLLR
                     DYHSPNLHWLADREGIRRIGLIDFQDALYGPAAYDVVSLLQDARVDVPDAMELRLLSH
                     YARLRRQEDPAFDMAGFAQAYAVLGAQRATKILGIFARLNRRDHKPGYLRHLPRIERN
                     VVRCLAHPALAELRQWYAANLPDLFA"
     assembly_gap    2622..2721
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2722..3464)
                     /gene="tsaE"
                     /locus_tag="PK812_10540"
     CDS             complement(<2722..3464)
                     /gene="tsaE"
                     /locus_tag="PK812_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016921777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HRJ70035.1"
                     /translation="MTAGPAQENPVAATETGAWPTHWIIEAKDQAATERVAAEIAGML
                     RPGDLVTLGGGLGVGKTTFARALIRALCGDDGLEVPSPTFTLIQTYDAPRHPIVHADL
                     YRIENPRELDSLGWNETADGAVLVVEWADRAGALLAPDRLDVSFAMEPDAPDVRILTL
                     TGHGTFSARLGLAKSIHLLLRGCGMAQAQRHHLQGDASTRVYERLHLPSARPAPTAIL
                     MIAPKRPDGPAIRNGRPYSQIAKLAEDVKP"
     gene            complement(3470..5992)
                     /locus_tag="PK812_10545"
     CDS             complement(3470..5992)
                     /locus_tag="PK812_10545"
                     /inference="COORDINATES: protein motif:HMM:NF024267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HRJ70036.1"
                     /translation="MAEGPIGTVGSSGVAARLRRAVRQLWRPLVALPALLLPTIGLAD
                     DLGLRLSTGSLPGRLEALKLDLSGPLGMLFVGLAIFSATIAVLHVRARRLWNDQFESQ
                     RAVIAELQIRSERAGLFLGSEPQVFVSWDGPGGQPEYEGDPRIVGETITTGRLLDFGN
                     WVAAGQAQALEAAVERLRTAGESFSLKLKSLGGSVIEADGQPVMGRAVMRLRNVTGER
                     QEIARIQEKLAASAGLITSLRALLDTIPQPVWLRRSDGALAFVNSAYAHAVEAKGADD
                     AMARGLELLERKTRDKVAHKHRQGENFRGAAKAVVAGDRRVLDVVDVIGTDGASGGIA
                     TDITELESARRDIEQQSEAHVRVLDQLPTAVALFDRQQRLSFYNRAFDRLWQLESGFL
                     GSRPTDMELLDRLRADGKLPEQSDFKEWKLGLQQSFQANETLEDVWHLPSGRALRVVT
                     SPSTKGGVTYLFDDVTEHMHLATSYNRLASMQGETLDSLAEGVAVFGSNGRLQLHNPA
                     FASLWGLSNAHLVDAPHIDLVAGRCATETHAVWSEIRAAVCGLPDERVNRTFDVRTRA
                     GKVLSLGLAPLPDGASLLTVEDVTSTANAARMLSERNQALEAAARFRSEFIHNVSFEL
                     RLPLTSVVGIAQLLATGTAGPLNERQRGYANDLMRATDAILALINDILDLASIDQGNL
                     DLKISKVDLRQSIEEASAGLKDRLGGAQVRLELRVAPEVDSILGDERRLRQILFNLLA
                     NAVACSDPGDSVVLEAEQGEEGIAIRVLDNGALPATGDAGADSQRRTDRGQSLRLSIV
                     RSLVELHHGRLMVEGRTDGKRQVVCILPAAHDPAQPPRRIAG"
BASE COUNT         1002 a         2026 c         2011 g          948 t
ORIGIN      
        1 agcgcggttc cgccggcatc gggccgggcc gccagcaacg ccagccgcgc caggatgccg
       61 gccggcgatt cgatcatccg caatccggcc gaatcggcga cgcgaacgtt ccagcgctcg
      121 agttctgcct gcacggcgcg cgcgagcatg cggtccggcg tcgccagtgc gatggtccgt
      181 cccggcgttt ccagcgcctc ccgcaggatc agggcgatgg caagcgcttc ctcgccgccg
      241 tcggccgcct ccaccagcgc catgccctcc ggaaccttgt cctgccgcag ggcgctccag
      301 gcgtcgctcg cttcggccgg acgaaagacc tccgaggcca gttggggccg ggtcgcgatg
      361 cgcgagtgcc cgtgcagcac gacgtcggca cgccctatgc cgatccgcgc cagggcgcgc
      421 ttcatgcttg cctggggatg ggcgaaacgc gtggcgagcg ccgcatcctc cgcgccgatg
      481 gcatcccagg ccgcggcatc gagggtagtg tccagcccgg gcaggacgac ggcgcccatc
      541 gtcagtccgg cgacgcaacg catcaattcg gccgttgcgg ataccgaccc ggtcgatccg
      601 gcaacgatga cgggtcggcc cgttcccgat tgcgccagcc gttgcgcctg ctccagcagc
      661 aggctgcgcg tgcgatgctc ggcatcgctc tggccgagct ccgcaagaat gaccggccac
      721 tgctgggccg cgatctcgag gaacttcgcg gtcagcgacc agtaggcatc gtggcgcgaa
      781 gggtcgaacg tcggcggcat gccctgtccg aggcgttcga gcgggacggc ctcgatgatg
      841 gtttcgtcga tcaggccggc aaggtcacca gccagcgcga aggcgtcggc acgcgagcgc
      901 gccacgaaga agggctcgcc atccccgtga ggtcgcgtcc cggtctcgat cgcccgccgc
      961 caggcgtcca ccagttcgaa gagtttcaac tggcgcacca gcggatcaat ggcgggggga
     1021 agaccaggca ggccggcagg tccgcccagc ccgctgccgg cggcgctgtc cggttccggg
     1081 tctcccagaa ggacgatgcg cggaagcatc agcgcgccct gcggcgcgcg cgccgcgatg
     1141 atcgaggcga agacctcggc cgcccggcgc gtcggcagaa acagcaaggc gtcggcgagt
     1201 tcgtccgggc gaagcgggtc cgaacgacca aacaaggtcc cgcgcaaaag cagttctacg
     1261 agcgtttcga ggaacggcgc gccggcgggg atggagatca cacgggggct tcggggcgcg
     1321 gctgacccgc tcaccgtgaa ctcccgatga tcgccgcttc ggcctcgaca atggcctcgg
     1381 gcgtgccgac atgcatgaaa cggccgtcca ggcgcaggcc aaaaagccgg cccgcggcca
     1441 tggcgcgatt gaacagcagc agcagggaaa aaacgtccgg cgtatcggaa aacaggcgcg
     1501 aatcgaggat cgcagcgccg caataggcga aaggcgcgac ctctcccggt cgcggccagc
     1561 gcagcctccc gtcctgttcc atcgccagat cgccgcgatt tccccagccg accgtcgata
     1621 ccgtcggcgc cagaagcagc aggatatcca tggtcgcggg gtcgaatgtc tcggccatcc
     1681 gcaccaggtt ggagcgcggc ccgtccatcc agatcgtgtc ggcgttgagg ctgaagaacg
     1741 ggccctcgcc cagcagcgcc agcgccttct tcacgccgcc cccggaatcg aggatcgccg
     1801 cgcgttcatc ggagatcgcg atgtcaggcg aatggcgcgc ccgcaaatgg gcttccagca
     1861 tgtcggcgag gtagtgcacg ttgaccaccg cacgggtgac cccggcttct tcgaggcggt
     1921 cgaaattgta gtcgatcagt gcctttccgg ccacttcgat catgggtttc ggacgcaagt
     1981 cggtcagcgg gcgcatccgc ttgcccaggc cagcggccag gatcatcgcg gtattgatct
     2041 gggggctggc catcgcggcg tcaggcaaaa aggtcgggca ggttcgccgc ataccactgg
     2101 cgcagttccg cgagcgcggg atgggcgagg cagcgcacga cgttgcgctc gatgcgcggc
     2161 agatgccgga ggtagcccgg cttgtggtct cgccggttca ggcgcgcgaa aatgccgagg
     2221 atctttgtcg cccgctgcgc ccccagaacc gcataggctt gcgcgaagcc tgccatgtcg
     2281 aaggccgggt cttcctgtcg tcgcaacctg gcatagtggc tcagcagccg aagctccatc
     2341 gcgtcgggca catcgacgcg cgcatcctga agcagcgaaa ccacgtcata ggcagcagga
     2401 ccgtagaggg cgtcctggaa gtcgatcagg ccgatgcgcc ggatgccctc gcgatctgcc
     2461 agccaatgca ggttcggaga atggtagtcc cgcagcagcc atgtgcccgg tccggccaga
     2521 agcggctgca acagcgccga ccacagatcg atgaaggcga tccgcgacgt cgccgagagg
     2581 gcgtggtgtc ccttgtgcgg cagataccaa tccggcatca gnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nggtttcacg tcttccgcca gcttggcgat ctggctgtac
     2761 ggtctgccgt tgcgaatggc gggcccgtcc ggccgcttgg gtgcgatcat caggattgcc
     2821 gtcggcgcgg gccgcgcgga gggaagatgc aaccgttcgt agacccgggt cgaagcgtcg
     2881 ccctgcaggt ggtgccgttg cgcctgggcc atgccgcagc cgcgcagcag caggtgaatc
     2941 gattttgcca ggccaagccg ggccgaaaac gtcccgtgcc ctgtcagcgt caggatgcgg
     3001 acatcaggag catcgggctc catggcaaag gaaacatcga gccggtctgg ggcaagcaac
     3061 gctcccgcgc ggtccgccca ctcgacgacc agcacggcac cgtcagcggt ttcgttccag
     3121 ccaaggctgt cgagctcgcg cgggttctcg atgcgataga gatcggcgtg gacgatgggg
     3181 tgacgcggcg catcgtaggt ctggatcagc gtgaatgtag gactgggaac ttccagccca
     3241 tcatcgccac acagggcgcg gataagcgcc cgggcgaagg tcgtcttccc gacgccaagc
     3301 ccgccgccga gcgtcacgag atcgcccggg cgaagcatcc cggcaatctc ggctgcgacc
     3361 cgttccgtcg cggcctggtc cttggcctcg atgatccaat gcgttggcca tgctccggtt
     3421 tcggtggcag cgaccgggtt ttcctgcgcg gggcctgcag tcatgaccgt taccccgcga
     3481 tgcgacgcgg cggctgagcg ggatcgtgcg ccgccggcaa aatacagacg acctgacgct
     3541 tcccatccgt tcggccctca accatcaggc gtccatgatg gagttccaca agcgagcgga
     3601 cgatcgacag ccgaaggctc tgtccgcggt cggttcgccg ctggctgtcg gcgccggcat
     3661 cacccgtcgc cggcagcgcg ccattgtcca gcacccggat ggctatgcct tcctcgccct
     3721 gctcggcctc gagcaccacg ctgtctccgg gatcggagca ggcgaccgca ttggccagca
     3781 ggttgaacag gatctggcgc aagcgccgtt cgtcgccaag tatcgagtcg acttcgggcg
     3841 ccacacgcag ttccagccgc acctgggcgc cgccgagccg gtccttgaga ccagcgctgg
     3901 cctcctcgat ggactgacgc agatcaactt tggaaatctt caggtcaagg ttgccctggt
     3961 cgatgctggc gaggtcgagg atgtcgttga tcagggcgag aatggcgtcg gtcgcccgca
     4021 tcaggtcgtt tgcatagccg cgttggcgct cgttcagcgg tccggcggtg ccggtggcga
     4081 gcagttgcgc gataccgacg acgctggtca gcggcaatct cagttcgaac gacacgttgt
     4141 ggatgaactc cgagcggaac cgtgcggcgg cttccagagc ctggttgcgc tccgagagca
     4201 tgcgcgcggc attggcggtc gaggtcacat cctccaccgt cagcagcgaa gcgccgtcgg
     4261 gcagcggcgc aagaccaagc gaaagcactt tgcctgcacg ggtgcgtacg tcgaaggtcc
     4321 ggttcacgcg ttcgtccggc agcccgcaga cggcggcgcg gatctcggac cacacggcat
     4381 gcgtctcggt ggcgcagcgg ccggcgacga gatcgatgtg tggcgcatca acgagatggg
     4441 cgttgctcag cccccagagg gatgcgaagg ccggattgtg caactgcagg cggccattcg
     4501 agccgaagac cgccacgccc tcggccagcg agtccagcgt ctcgccctgc atgctggcaa
     4561 gccggttgta gctggtcgcc agatgcatgt gttcggtgac gtcgtcgaac aggtaggtga
     4621 cgccgccctt ggtgctcggg ctggtgacca cgcgcagggc gcgaccgctc ggcaggtgcc
     4681 agacgtcttc aagggtctcg ttggcctgga agctctgctg taggccgagc ttccattcct
     4741 tgaagtccga ctgttcaggc aattttccat ccgcgcgcaa gcggtcaagc agctccatgt
     4801 cggtgggacg gctgcccagg aaccccgact ccagctgcca cagccggtcg aacgcccggt
     4861 tgtagaagga cagccgctgc tgccggtcaa acagcgcgac tgccgtcggc aactggtcga
     4921 gcacccggac gtgcgcctca ctctgctgct cgatgtcgcg ccgggcggat tcgagttcgg
     4981 ttatgtccgt ggcgattccg ccgctcgcgc cgtcggtgcc aatcacgtcg acgacgtcga
     5041 gaacgcgccg gtcgccagcc acgacggcct tggccgcgcc gcggaaattc tccccctggc
     5101 ggtgtttgtg cgccactttg tcccgggtct tgcgctcgag cagttccaac ccgcgcgcca
     5161 tcgcgtcatc ggcgcctttg gcctcgaccg catgggcgta ggcggaattc acgaacgcca
     5221 gcgcgccgtc gctcctgcgc agccagaccg gctgcggaat ggtgtccagc aaggcccgaa
     5281 gcgacgtgat gagcccggcc gaggcggcga gcttttcctg tatccgcgcg atctcctggc
     5341 gctccccggt gacgttgcgc agtcgcatca cggcgcgccc catcacgggc tggccatccg
     5401 cctcgatgac cgatccgcca aggctcttca gtttcaggct gaaggactcg ccggccgttc
     5461 taaggcgttc caccgcggct tcaagcgcct gcgcctgacc ggctgccacc cagttgccga
     5521 agtcgagaag gcgcccggtc gtgattgtct cgccgacgat cctcgggtcc ccttcgtatt
     5581 ccggctggcc gcccggaccg tcccagctca cgaacacctg cggttccgac ccgagaaaca
     5641 gtccagcgcg ctccgagcgg atctgcaatt ccgcaatcac ggcacgctgg ctctcgaact
     5701 gatcgttcca gaggcggcgt gcacggacat gcagaacggc gatggtggcg gaaaagatgg
     5761 ccagaccaac gaaaagcatc cccagcggtc cgctgaggtc gagcttcagc gcctccagcc
     5821 gtcccggcag ggagccggtg gacaaacgca atccaagatc gtcggccagg ccaatggtgg
     5881 ggagcagaag cgcgggcaac gccacgagag gacgccacag ctggcgcacg gcgcgccgaa
     5941 gccgtgcggc gacaccgctt gatcccactg ttcctatggg cccctcggcc atgcccggct
     6001 cccgtcctcg cccctggcgc cagacccgtc gttgcgccgc cgccccaagc ggcgtccgta
     6061 gcggccggca atccggagcg cgccccg
//
LOCUS       DAOMXN010000158         6082 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_6860_length_6082_cov_9.638792, whole genome shotgun sequence.
ACCESSION   DAOMXN010000158 DAOMXN010000000
VERSION     DAOMXN010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 6082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6082
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6860_length_6082_cov_9.638792"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PK812_10550"
     CDS             <1..234
                     /locus_tag="PK812_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HRJ70037.1"
                     /translation="LAAALERSAGPVVLVSNETGLGIVPATPLGRDFRDAQGRLNQAM
                     AGVCDGVQVMVAGLPIVLKPAPQGARLRLRLED"
     gene            complement(299..1261)
                     /locus_tag="PK812_10555"
     CDS             complement(299..1261)
                     /locus_tag="PK812_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002966346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRJ70038.1"
                     /translation="MKAKIFSGVIPALMTPCTAERQPDFAALVRKARQLIAEGMSAVV
                     YCGSMGDWPLLTDAQRMEGVERLVKAGVPVIVGTGAVNTASAVAHAAHAQKVGAQGLM
                     VIPRVLSRGSVIQAQKAHFKAILAAAPDLPAVIYNSPYYGFATRADLFFALRAEHPNL
                     VGFKEFGGAADLRYAAEHITSRDDGVTLMVGVDTAVFHGYVNCGATGAITGIGNVLPK
                     EVLHLSNLAEAAAKGDADARQRAQELEQALAVLSSFDEGPDLVLYFKHLMVLKGDREY
                     TLHFNETDALTDSQRGYAEAQFKLFNAWYADWSRLPGAVKKYAA"
     gene            complement(1435..2499)
                     /gene="mutY"
                     /locus_tag="PK812_10560"
     CDS             complement(1435..2499)
                     /gene="mutY"
                     /locus_tag="PK812_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013912928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HRJ70039.1"
                     /translation="MLPSDATDATPEAAALLAWYDRHRRDLPWRAAPGSPADPYRVWL
                     SEIMLQQTTVAAVRPYFAAFLARWPRIEALASAPLEEVLSAWAGLGYYSRARNLHACA
                     QRVMDRHGGRFPAEEAILRTLPGIGAYTAAAISAIAFARRATVVDGNVERVIARYFAV
                     EAPIPAAKARIRRLADDLTPGTRPGDYAQAMMDLGATVCTSRSPDCGRCPLAQGCMAH
                     RRGIAAELPRKAPKEPKPVRFGAAYLAFRADGTVLIETRPAQGLLGGMPGLPTTDWRA
                     EAPGMTEAAPAAASWREAGAITHVFTHFRLELTVFVAECGPRPTVSPAMRWERPEAIR
                     PSLPTVMRKALDRGMAAKGS"
     gene            2613..3131
                     /locus_tag="PK812_10565"
     CDS             2613..3131
                     /locus_tag="PK812_10565"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HRJ70040.1"
                     /translation="MSALVPGQIAPVYARRGFGGADLVVHWPEIAGSGVARHSRPLSL
                     DWPKGAENNGRGATLVVACSGGFALDLQQMAPILIERINRRLGWRCVARLVIRQMPVR
                     PPGSPAKRPKPAPEDVKAAGRIAAGIAAEGLRDAITRLGAATIARARSRSERLPSGPA
                     TLERLPDPIKMP"
     gene            3199..3897
                     /locus_tag="PK812_10570"
     CDS             3199..3897
                     /locus_tag="PK812_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006330664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HRJ70041.1"
                     /translation="MTRVALSRSGALVAVLGALLAVSACDSGQQSKAPAPPTADAARK
                     GAEPTVDVAELMKAGPLPEQVLGNSAAPVTIVEYASMTCVHCATFHNESFGHLKQQYI
                     DTGKVKYIFREFPLDPLAYAGALLARCAGEGKYFPMLELLFKQQRNWAYAEQPEAALL
                     QTVRQAGFTQESYTACLQDQATYDALKQIRQRAVEKFAVESTPTFFINGKIQRGAVSP
                     QQLDKLIEPHLPAK"
     gene            3920..4579
                     /locus_tag="PK812_10575"
     CDS             3920..4579
                     /locus_tag="PK812_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019169797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HRJ70042.1"
                     /translation="MPQSRRSLLLMASAMLMASWAPSHGQVGKSDGQVDPVELLKAAA
                     LEDHVLGRSDAPVTVIEYASMSCPHCAMFHVQTLPELKRDFIDTGKVRFIFREFPFDG
                     VALAGSMLARCTGPQQFFDAVEVLFREQQHWAFSSDPESALKAIAFSRYGFTQESFDR
                     CLQNQKMYDAVLAGRKRAYEQFGVASTPTIFVNGVRYRGALSPADMAAAIANAPATGK
                     Q"
     gene            4619..>6082
                     /locus_tag="PK812_10580"
     CDS             4619..>6082
                     /locus_tag="PK812_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRJ70043.1"
                     /translation="MKFDRLRIIGFKTFIDPTELVIETGLTGVVGPNGCGKSNLVEAL
                     RWVMGETSHKSMRASGMDDVIFSGSGQRPARNHAEVMLRVDNGAREAPAAFNDADFLE
                     ISRRIEREAGSTYRINGREVRARDVQLLFADAASGARSPSMVRQGQISEIISAKPQAR
                     RRILEDAAGIAGLHARRHDAELKLKAAEENLARVVDVLTHIDGQIDGLKRQARQAERY
                     RTVAADIRRAEAVVRQREWHGASALVEQAGRQLDLDTRAVASAMTEQGEAERQRAVAQ
                     HAIAPLRQEEAKAGELLQRLVIAREALDGEERRAKSRQEELDRRIAELARDLERARRM
                     CDDATAALTRLTSEQTLLSAESGGSDAEPQARERLSSAEAEVQQAEATLQEIQTALAE
                     ATARRSVAESGLAEANARLARLKPQLAETERQAAQLAASASGAPDLEVLRASVSAAAA
                     ALESSEAGAQQAEVAHLAVDLLARHARFHRAAQVFGID"
BASE COUNT         1006 a         2022 c         2044 g         1010 t
ORIGIN      
        1 ctcgccgcgg cgctggaacg atccgccggc ccggtcgtgc tggtctccaa cgagaccggt
       61 ctggggattg tcccggcgac gccgctcggt cgcgacttcc gggatgcgca aggccggctc
      121 aaccaggcga tggccggcgt ctgcgacggg gttcaggtca tggtcgccgg gctgccgatc
      181 gtgctcaagc cggcgcctca gggcgcgcgt ctgcgcctcc gattggagga ctgatcccgg
      241 cgcggccatc gggtatcgcc gatgatatcg gccccctctg ctgtccgggc gcctgcgatc
      301 aggccgcgta tttcttcacc gcgcccggca ggcgactcca gtccgcatac caggcgttga
      361 acagtttgaa ctgggcctcg gcatagccgc gctggctgtc cgtcagggcg tcggtctcgt
      421 tgaaatgcag ggtgtattcc cggtcgccct tcagcaccat cagatgcttg aaatacagca
      481 cgaggtccgg tccctcgtcg aaggacgaga gcaccgccag cgcctgctcc agttcctgcg
      541 cccgctggcg cgcgtcggca tcgcccttgg ccgcggcctc ggccaggttg gacaggtgca
      601 gcacttcctt cggcagcacg ttgccgatgc cggtgatcgc gccggtcgcg ccgcagttca
      661 catagccgtg gaacaccgcc gtatcgacgc cgaccatcag cgtgacgccg tcgtcccggc
      721 tggtgatgtg ctcggcggca tagcgcaggt cggccgcccc gccgaattcc ttgaagccga
      781 caaggttcgg atgctcggcc cgcagggcga agaacaggtc cgcgcgcgtc gcgaaaccgt
      841 agtaggggct gttgtagatc accgccggca gatcgggcgc cgctgcgagg atggccttga
      901 aatgcgcctt ctgcgcctgg atgacggagc cgcgcgacag cacgcgcggg atgaccatca
      961 ggccctgcgc gccgaccttc tgcgcgtgcg cggcatgagc gaccgccgag gccgtgttga
     1021 cggcgccggt gccgacgatg accggcacgc cggccttcac cagccgctcc acgccttcca
     1081 tgcgctgcgc gtcggtgagc aggggccagt cacccatcga tccgcagtag acgacggcgg
     1141 acatgccctc ggcgatcagc tgtctggcct ttcgcaccag tgccgcgaag tcgggctgcc
     1201 gctcggccgt gcagggcgtc atcagtgccg ggatcacgcc ggaaaaaatc ttggccttca
     1261 tggccacctc ctcatcgctg cgccgcgtca cggtgcgggc atggcgcgct tctcttgttg
     1321 accaaaaaac aggcgcgcgc gcaacggcgt gatcgatccg gttggtttcg atggagcgcg
     1381 catcgggtgt tcgtgtccga aaggcctgcg ctcggccctc gaggactgcg tgttctacga
     1441 tcctttcgcc gccatgccgc gatccagcgc cttgcgcatc accgtcggca ggctgggccg
     1501 gatcgcctcg ggacgttccc agcgcatcgc tggcgaaacc gtcggccttg ggccgcattc
     1561 tgccacgaag accgtcaatt cgagacggaa atgtgtgaaa acatgcgtga tcgcgccggc
     1621 ctcccgccag gacgcggcgg ccggggccgc ttctgtcatc cccggtgcct cggcgcgcca
     1681 gtccgtcgtc ggcaatccgg gcatgccgcc gagcaggccc tgggccggcc gcgtctcgat
     1741 cagcacggtg ccgtccgctc gaaacgctag gtaggcagca ccgaatcgga ccggtttcgg
     1801 ttctttcggc gcctttcgag gcagttcggc ggcaatgccg cgccggtgcg ccatgcagcc
     1861 ctgtgccagc gggcaccgcc cgcaatccgg gctgcggctg gtgcagaccg tcgcgccgag
     1921 gtccatcatg gcctgcgcgt aatctcccgg acgggttccc ggcgtcaaat cgtccgcgag
     1981 cctgcgaatc cgggctttcg cggccggaat cggcgcttct acggcaaaat atcgcgcgat
     2041 cacgcgttcg acattgccgt cgaccacggt cgccctgcgc gcgaaggcga tcgccgaaat
     2101 cgccgcggcg gtataggcgc caatgccggg caatgtgcgc aagatggcct cttccgccgg
     2161 aaatcgcccg ccgtggcggt ccatcacccg ttgcgcgcag gcgtgcagat tccgcgcccg
     2221 ggaatagtat cccagcccgg cccatgccga gagcacctct tccagcgggg ctgatgccaa
     2281 agcctcgatt cggggccagc gcgccagaaa agccgcgaaa tagggccgga cagccgcgac
     2341 cgtggtctgt tgcagcatga tctcggacag ccacacccga taaggatcgg cagggcttcc
     2401 cggtgcggca cgccagggca ggtcgcggcg atggcgatca taccaggcaa gcagcgcggc
     2461 agcttcaggc gttgcatcgg tggcgtcaga cggtagcatg gcgccaccat ggtcgcatgc
     2521 agccgtcgtg gcgagtccct cagagtgaaa ttgcaaagtc agtggccgac gatccatcac
     2581 ctccgctgcg ttccaaacga gggccggcgc ggttgtccgc gctggtgccg ggccagatcg
     2641 ccccggtcta cgccagacgc ggcttcggcg gtgctgatct tgtcgtgcat tggccggaga
     2701 tcgccgggtc cggcgtggcc cgccacagcc ggccactgtc gctggattgg ccaaagggag
     2761 ccgagaacaa cggccggggc gcgaccctcg tcgttgcctg ctcggggggc tttgcgctgg
     2821 atctccagca gatggcgccg atcctgatcg aacgcatcaa tcgcaggctc ggctggcgct
     2881 gcgtggcgcg gctcgtgatc cggcaaatgc ccgttcgccc gcccggttcg cccgccaaga
     2941 gaccaaagcc tgctcccgaa gatgtgaaag ccgctggccg cattgccgcc ggaattgccg
     3001 ccgagggctt gcgggacgcg atcacgaggc ttggagccgc gacaattgcc cgtgcgcggt
     3061 cgagatcaga gcggctaccg agcgggcctg cgaccctgga gcggttgcca gaccctatca
     3121 aaatgcccta ggcagacatc gcttcgctgg ttctgctcgc ggcgcgtgtc ccggacaaga
     3181 aacggactgg actttacgat gactcgagtt gctctttccc gttccggcgc cctggttgcg
     3241 gtcctcgggg ccctgctggc ggtttccgcc tgcgattccg gccagcagtc caaggcgccg
     3301 gcgcctccga cggcggatgc agcgaggaag ggcgcggaac cgactgtcga tgtggcggag
     3361 ctcatgaagg cgggcccctt gccggagcag gtgcttggca attccgccgc gccggtcacc
     3421 atcgtcgaat atgcgtcgat gacgtgtgtg cactgcgcga cgttccataa cgagtctttc
     3481 ggtcatctca agcagcagta catcgacacc ggcaaggtga agtacatctt ccgggagttc
     3541 cctctcgacc cgctggccta tgccggggcg ctgctggcgc ggtgtgcggg cgagggcaag
     3601 tacttcccca tgctcgaact gctgttcaaa cagcagcgca actgggccta tgccgagcag
     3661 cccgaggcag ccctgctgca gacggttcgg caggccggct tcacgcagga gagctatacc
     3721 gcctgcctgc aagatcaggc gacctatgac gcgctcaagc aaattcgcca gcgtgccgta
     3781 gagaaattcg cggtcgaatc gacgcccacg ttcttcatca acggcaagat tcaacgcggg
     3841 gcggtttcgc cgcagcagct ggacaagctg atcgagccgc acctgccggc gaaatagtgg
     3901 gctggaaagg aagcgcgcca tgccgcagtc tcgcagatcc cttctcctga tggcgtcggc
     3961 catgctgatg gcgtcctggg cgccatcgca cggtcaggtc ggcaagtcgg acggacaggt
     4021 ggacccggtc gagctgctga aagccgcggc actcgaagat cacgtgctcg gccgttcgga
     4081 cgcgccggtg accgtcattg aatacgcctc gatgagctgc ccgcactgcg ccatgttcca
     4141 tgtccagacg ctgccagagc tgaaacggga tttcatcgac accgggaagg tgcgcttcat
     4201 attccgcgag tttccgtttg atggcgtcgc gctcgccgga tccatgctgg cgcgatgcac
     4261 cggcccgcag cagttcttcg atgcggtcga ggtgctcttt cgcgagcagc agcactgggc
     4321 gttttcgagt gatccggaaa gcgccctgaa agccattgcg ttctcccgct acggtttcac
     4381 acaggaaagc ttcgaccgct gcttgcaaaa ccagaaaatg tatgacgccg ttctggctgg
     4441 aaggaagcgt gcctacgagc agttcggcgt cgcgtccacg ccgacgatct tcgtgaacgg
     4501 cgtccgatac cgtggcgcgc tttctcccgc cgacatggct gcggcgatcg ccaacgctcc
     4561 ggcgaccgga aaacaatagc ccgccgctgt gcgggtgttt cagagcgatt gaccccgagt
     4621 gaagttcgac cggctgagaa tcatcggctt caagaccttc atcgatccca cagaactggt
     4681 gatcgagacg ggactgaccg gcgtcgtcgg tcccaacggt tgcggcaagt cgaacctggt
     4741 cgaggccctg cgctgggtca tgggcgagac ttcgcacaag agcatgcgcg cttcgggcat
     4801 ggacgacgtg atcttttcgg gatcagggca gcgtccggcg cgcaaccatg cggaagtcat
     4861 gctgcgcgtc gacaacggcg cgcgtgaggc gccggcagcc ttcaacgacg cggactttct
     4921 cgagatttcc cgccggatcg agcgcgaggc cggctcgacc tatcgcatca acggacgtga
     4981 ggtgcgtgcg cgtgacgtgc agctgctctt cgcggatgcc gcgagcggcg cgcgctcgcc
     5041 ctcgatggtg cgtcaggggc agatcagcga aattatctcc gccaagcccc aggcgcggcg
     5101 ccgtatcctt gaggacgctg ccggaatcgc tggcttgcac gcccggcgac acgatgcgga
     5161 actcaagctg aaggccgcgg aagaaaacct cgcgcgggtc gtcgacgtcc tgacccatat
     5221 cgacggtcag atcgacggcc tgaagcgtca ggccaggcag gccgagcgct atcgcaccgt
     5281 cgccgcggat atccgccggg cggaagcggt ggtgcgccag cgcgagtggc acggcgcgag
     5341 cgcgcttgtc gagcaggcgg gccggcagct tgatcttgac acccgcgccg tcgcttcggc
     5401 catgaccgaa cagggcgagg ccgagcgcca gcgcgcggtc gcccagcatg ccatcgcgcc
     5461 gttgcgacag gaggaggcca aggcgggcga gctcctccag cgcctggtga tcgcgcgcga
     5521 ggcactggac ggcgaggaac ggcgcgccaa gtcccgtcag gaggagcttg accgacggat
     5581 cgccgagctt gcgcgcgatc ttgagcgcgc caggcgcatg tgcgacgacg cgacggcggc
     5641 gctgacgcgc ctgacatccg agcagacgct gctctcggcg gaatccggcg gttcggacgc
     5701 cgaacctcag gcgcgcgagc gcctgtcctc agcagaagcc gaggtgcagc aggcggaagc
     5761 gacgctgcag gagatccaga ccgcccttgc cgaggcgaca gcccggcgat ccgtcgccga
     5821 aagcggactt gcggaggcga acgcccgtct tgcccgtctg aagccccagc ttgctgaaac
     5881 cgaacggcag gcggctcaac tcgcggcatc cgcttccggc gcgcctgacc tggaggtgct
     5941 gcgcgcctcg gtgtcggcag cggcggccgc gctggagtcg agtgaagcgg gggcgcaaca
     6001 agccgaggtc gcgcacctcg cggttgacct gctcgcgcgt cacgcccggt tccaccgagc
     6061 agctcaggtc ttcggcatcg at
//
LOCUS       DAOMXN010000159         6081 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_6862_length_6081_cov_9.405410, whole genome shotgun sequence.
ACCESSION   DAOMXN010000159 DAOMXN010000000
VERSION     DAOMXN010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 6081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6081
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6862_length_6081_cov_9.405410"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1018)
                     /locus_tag="PK812_10585"
     CDS             complement(<1..1018)
                     /locus_tag="PK812_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HRJ70044.1"
                     /translation="MSFEPAFDAFAARFDHGESQCLSARLVADLTTPVSARLKLAAAF
                     SGPNILLESVEGGAVRGRYSIIGLAPDLTWRCRDGVAALSVDGETFTPDALPPLQSLR
                     ALLKVSALTLAEDLPPMACGVFGYLGYDMVRQMERLPGEKPDPLGVPDALLIRPRLVV
                     VFDAVKDELIVVTPVRPKAGGSARAAYESACDLLEQALAALEAPLRHEVSAEEAERAA
                     RALAQANPLSNTPPADYLAMVARAKEYILAGDIFQVVLSQRFSQGFDLPAFSLYRALR
                     RVNPSPYLCYLDFMSFQIVCSSPEILVRVRDGKVTIRPIAGTRRRGTTAAEDRDLEVE
                     LLADP"
     gene            complement(1099..1833)
                     /locus_tag="PK812_10590"
     CDS             complement(1099..1833)
                     /locus_tag="PK812_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70045.1"
                     /translation="MIGLAGGDDVGDSVLWVALTVGLFVVPVAIGSAVIARLQQTFYR
                     RLEFIRSYRWPPALLDQLKKHHPDFTRKETALIAQGLKQFFIAHLRSGRAYVAMPSQA
                     ADELWHEFILYTRAYQEFCDQAFGRFLHHTPAVALAPARQRTNEGLRRVWWWSCKEES
                     INPTNPTRLPLLFALDKKLNIPNGYHYTADCEQLRKNGVPGTQCGGDFSSSSFDGSTT
                     GMGDGGTGVNDGADGGGSDGGGCGGD"
     gene            complement(1863..2504)
                     /locus_tag="PK812_10595"
     CDS             complement(1863..2504)
                     /locus_tag="PK812_10595"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015821301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HRJ70046.1"
                     /translation="MRIPSWFPPWLRRRLNPNNLTRLHLERLSEKHGFDIGDFSYGAP
                     KVRFAGMGAGLCIGKYCSIADRVDIFLGGNHRTDFVTTYPFTALPGLWPEGEGLTDFT
                     ATRGDVRIGHDVWIGSGASILSGVTVGNGAVIAARAVVTRDVPPFAIVGGNPAKVIRF
                     RFAPEIIVQLQETAWWDLPVSSVRKLVRVLQSDNMDAAIEAVRMERERLQLAR"
     gene            complement(2561..4546)
                     /locus_tag="PK812_10600"
     CDS             complement(2561..4546)
                     /locus_tag="PK812_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70047.1"
                     /translation="MQNILKRTSMAASVLALGAGIYAGASHMAAESLRARLMAATALS
                     GAAASVDKIAVNPLTGSFRIDGMKVRQPGGATVEIGRLTHEGSSFALVTQAFAQQAHS
                     FTVDEMKIVGKIGSLRISKIAVEGASLDQSALKSLFESGASASLIDRLKGLSFRSARA
                     PEIAWVFQAGGDASAQTFRDVTFEGMRNGRLERASAASGTLESRSGKGPVVTGGHGLY
                     DIRGLDTVLVARFMFDKAAPNEALATVQDSILVENFKVSADSLVEYVIGRMEVGAIRL
                     RPLSIPLADLLLKMPSDGQPKLPPEEVKVLVPALFGLYDAFSDDGFAVSDIRINLPKM
                     PGAGGVIARMSGSFGGGKAPAGYKIEGVAGNGPGFMFKLASMGWEGFSLKPTLNGALK
                     AIAAGDTDLNKVDPRDLMPKLGAQYLRGLEFEFPDPKPVRGQPPGRVAGGIGAFEFSA
                     KNEIKGIPTEMTMVYDKVALKVPMTTNDDGFKNLLALGYTAIDLSARANLKWNEARKE
                     INLTELVLSGVNMGSARLTATVGNIGRDVFEAELPMMQVAALGATAKSVALKVENTGL
                     AERIFEMQAKQQRRKADEIRAEMGSMAALGIPALLGPSDDAKALANALGRFLARPRNL
                     TIEASAKNAGGIGIPDMATLSNPQQALTLVNLKASAD"
     gene            complement(4712..5050)
                     /gene="erpA"
                     /locus_tag="PK812_10605"
     CDS             complement(4712..5050)
                     /gene="erpA"
                     /locus_tag="PK812_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HRJ70048.1"
                     /translation="MTMETTPVQVTESAARRIARILESEPSGSMLRVSVKGGGCSGFQ
                     YTFDIDSSRTPDDLAVSRDGVTVLIDSVSLPFLSGSQIDFVDDLMGQSFRVHNPNATA
                     SCGCGTSFSI"
     gene            5151..>6081
                     /locus_tag="PK812_10610"
     CDS             5151..>6081
                     /locus_tag="PK812_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HRJ70049.1"
                     /translation="MRFYRERQLSAFAAVASRSRGRLVREPASPTRTEFQRDRDRIVH
                     STAFRRLKHKTQVFVEHEGDHYRTRLTHTIEVAQIARAVARALAVDEDLAEALALAHD
                     LGHPPFGHAGEDVLDECLNDYGGFDHNAQALRIVTRLERRYAAFNGLNLTWETLEGLV
                     KHNGPLLDSAGKPVKRYEENGVPGAILEFDAVFPLNLSTFAGVEAQIAAIADDIAYNA
                     HDIDDGLRAGLFDLESLVETDFLGALIAEIRGEWPMLDTVRTGHELVRRVITRFVEDV
                     IAEAERRLVALAPESVEAVRQAAAPVVAFSPAMA"
BASE COUNT         1091 a         1961 c         1993 g         1036 t
ORIGIN      
        1 tggggtcggc cagcaactcg acctccaggt cgcggtcctc cgccgccgtg gttccccggc
       61 ggcgcgttcc ggcgatcggc cggatcgtga ccttgccgtc gcgcacgcgg accaggattt
      121 ccgggctgga gcacacgatc tggaaagaca tgaaatcaag gtagcaaagg tagggcgagg
      181 ggttcacccg gcgcaaagcg cggtacagcg agaaagccgg caggtcgaac ccctgagaaa
      241 agcgctgcga cagcacgacc tggaagatat cgccggccag gatgtattcc ttggcccgcg
      301 ccaccatggc gaggtagtcg gcgggcggcg tgttcgagag cggattggcc tgggccagcg
      361 cccgcgcggc ccgttccgcc tcttcggcgc tgacttcgtg gcgcagcggg gcttcgagtg
      421 ccgccagtgc ctgttccaga aggtcgcacg cgctctcgta agcggcccgc gcgctgcccc
      481 ccgctttggg ccggaccggc gtgacgacga tcagttcgtc cttcaccgca tcgaacacca
      541 cgacaaggcg cggacggatc agcagggcgt ccggcacgcc gagcggatcc ggcttttcgc
      601 ccggcaggcg ttccatctgg cgcaccatgt catagccgag atagccgaag acaccgcagg
      661 ccatcggcgg caggtcttcg gccagcgtca gggcggaaac cttcagcagc gcacgcaggc
      721 tctgcaaggg cggcagtgcg tccggcgtga aggtctcgcc gtcgacggac agcgcggcca
      781 cgccatcgcg gcagcgccag gtgaggtcag gcgccagtcc gatgatcgaa tagcggccgc
      841 gcacggcgcc gccctcgacc gattccagca ggatgttggg acctgaaaag gcggccgcca
      901 gtttcagccg cgccgaaacc ggcgtcgtca gatcggcgac cagccgggcc gacaggcact
      961 ggctctcgcc atggtcgaag cgcgccgcga aggcgtcaaa ggcgggttca aagctcatgg
     1021 gcgctgatta caccgcgagc gacgcgcggc caacgcttct ttgggttggc tccggcctgc
     1081 gcgatgcgct gttccgcctc agtctccgcc gcagccgccg ccgtccgatc cgccgccgtc
     1141 ggcgccgtcg ttgaccccgg tgccgccgtc gcccatgcct gttgtggagc cgtcgaagct
     1201 cgaagaggag aagtcgccgc cgcactgcgt gcccggcacg ccgttcttgc gcagctgctc
     1261 gcaatcggcc gtgtagtgat agccgttcgg gatgttcagc ttcttgtcga gggcgaagag
     1321 caacggcaac cgggtcggat tggtcggatt gatgctctct tccttgcaac tccaccacca
     1381 gacccggcgc agcccttcat tggtccgctg tcgcgccggc gcaagcgcca ctgccggcgt
     1441 atgatggagg aagcgaccga aagcctggtc gcaaaactcc tgataggcgc gggtatagag
     1501 gatgaattca tgccagagtt cgtcggcagc ctgcgatggc atcgcgacgt acgcgcgacc
     1561 gctgcgcagg tgggcaatga aaaattgctt cagcccctgc gcaatgaggg cggtctcctt
     1621 ccgcgtgaag tcggggtggt gtttcttgag ctggtcgagc agggccggtg gccatcggta
     1681 gctccggatg aactcaagcc gccggtagaa cgtctgctgc agtctcgcga tgacggcgga
     1741 gccgatggcc acaggcacca cgaagagccc gacggtcagg gcaacccaca gcacactatc
     1801 tcccacgtcg tcccctcccg ccagcccgat cattatcaaa aaagccggtt ccggcgagcc
     1861 cgtcaccgcg cgagttgaag ccgctcacgc tccatgcgga ccgcctcaat cgcggcgtcc
     1921 atgttgtcgc tctgaagcac gcgcacgagc ttgcgcacgc tggacacagg aagatcccac
     1981 caggcggttt cctgaagctg gacaatgatt tccggcgcga acctgaagcg gatcaccttg
     2041 gccggattgc cgcccacgat ggcgaagggc ggcacatcgc gggtcaccac ggcgcgggcg
     2101 gcgatcaccg cgccgttgcc gacagtcacg ccggacagga tgcttgcgcc cgatccgatc
     2161 cagacatcgt gtccgatgcg cacatccccg cgcgtggcgg tgaagtcggt cagaccctcg
     2221 ccctcgggcc agaggccggg cagggcagtg aacgggtagg tcgtgacgaa gtcggtgcga
     2281 tgattgccgc cgaggaaaat atcgacacgg tccgcgatcg agcagtattt gccgatgcac
     2341 aatccggcgc ccatccctgc gaagcgcacc ttcggcgcgc cgtaggaaaa gtcgccgatg
     2401 tcaaagccgt gcttttccga caggcgctcc aagtgcagcc gggtcagatt gttaggattg
     2461 aggcggcggc gaagccaggg cgggaaccag gacggaatgc gcatgcggcg gaatatcgca
     2521 aagggtgaca gcgccttatt gcgccgtcac ccgcgcgaaa tcaatccgcc gaggccttca
     2581 ggttgaccag agtcagcgcc tgctgcgggt tgctcagcgt cgccatgtcc ggaatgccga
     2641 tacccccggc gttcttggcg ctcgcttcga tcgtcagatt gcgcggccgg gccaggaagc
     2701 ggcccagagc gttcgccaga gccttggcgt cgtcggatgg accgagcagt gccgggatgc
     2761 cgagcgcggc catggacccc atctcggcgc ggatctcatc agccttgcgg cgctgctgct
     2821 tggcctgcat ctcgaagatg cgctcggcca ggcccgtgtt ctccaccttg agcgcgacgg
     2881 acttggctgt cgcccccagc gcagccacct gcatcatggg cagttcggcc tcgaacacgt
     2941 cgcgtccgat gttgccgacg gtggccgtga gccgggccga ccccatgttg acgccgctga
     3001 ggaccagttc ggtcaaattg atttccttgc gcgcctcgtt ccacttgagg tttgcgcgcg
     3061 ccgagaggtc gatggcggta tagccaagag ccagcaggtt cttgaagcca tcgtcgttgg
     3121 tggtcatcgg aaccttgaga gccaccttgt cgtagaccat ggtcatctcg gtggggatgc
     3181 ccttgatctc gttcttggcg gagaactcga acgccccaat gccgccggcc acgcggccgg
     3241 ggggctgtcc gcgaaccggc ttgggatcgg ggaactcgaa ttccagtccg cgcaggtact
     3301 gcgctccaag ttttggcatg aggtcgcgcg ggtcgacctt gttcaggtcg gtgtccccgg
     3361 cggcgatggc cttcaaggcg ccgttgaggg tcggcttcag cgagaaacct tcccagccca
     3421 tcgaggccag cttgaacatg aaaccgggac catttccggc cacgccttca atcttgtatc
     3481 cggcaggcgc cttgcctccg ccgaacgaac cggacatgcg ggcgatcacg ccgcccgcgc
     3541 ctggcatttt cggcaggttg atccggatgt cgctcaccgc gaatccgtca tcggaaaagg
     3601 catcgtacag cccgaacagg gccggaacga gcaccttgac ttcctcgggg ggcaatttgg
     3661 gctgaccgtc cgagggcatc ttcaagagca aatcggcgag cggaatggac aacgggcgca
     3721 ggcgtatggc gccgacttcc atccggccaa tcacgtactc aacaagactg tcggccgaga
     3781 ccttgaaatt ctcgacaagg atcgagtcct ggaccgtcgc gagcgcctcg ttcggcgcgg
     3841 ccttgtcgaa catgaagcgg gcgaccagca ccgtatcgag accgcggatg tcgtagaggc
     3901 catgcccgcc ggtcaccacg ggccccttgc ccgagcggct ctcaagcgtt ccagaggctg
     3961 cggaagcccg ttccagccgt ccgttgcgca tgccttcgaa cgtcacgtcg cggaacgtct
     4021 gggccgaggc atcgccaccg gcctggaaga cccaggcgat ttcaggcgcg cgcgcggagc
     4081 ggaatgaaag ccccttgagc cgatcgatca acgaggcgga tgcgccggat tcgaacagtg
     4141 acttgagcgc gctctgatcg agacttgccc cctcgacggc gatcttgctg atgcgcagtg
     4201 atccgatctt gccgacgatc ttcatctcgt cgacggtgaa ggaatgggcc tgctgggcga
     4261 aggcctgcgt caccagcgcg aagctgctgc cctcatgggt caatcggcca atctcgaccg
     4321 tggctccgcc gggctggcgg accttcatgc cgtcgatgcg gaaggaaccg gtgagcgggt
     4381 tgaccgcgat cttgtccacc gatgccgcgg cgccggacag cgcagtcgcc gccatcagcc
     4441 tcgcccgcag cgattcagcg gccatgtgcg aggcgccggc gtaaatgccg gcgccaagcg
     4501 ccagaacgct ggcggccatg cttgtccgct tcaggatatt ctgcatcagt tcgtctccgt
     4561 tgcgcgacgc cggccccacg gcgagtgagt aaaacgataa ggtcacgata aggttgagaa
     4621 atgactattt tctcgggagt gcacagccgc aacggccccg gcaagcggcc gggtcagtag
     4681 gagcctcaat cgcggcgaag ctgtggcagg atcagatgga aaacgacgtc ccgcacccac
     4741 aggaggcggt ggcgttgggg ttgtgcaccc ggaacgactg ccccatcaga tcatcgacga
     4801 aatcgatctg cgaaccggac aggaagggca gggagacgct gtcgatgagt acggtgaccc
     4861 cgtcccggct gaccgccagg tcatcggggg tacggctgct gtcgatgtcg aacgtgtact
     4921 ggaatccgga gcaaccgccg cccttgacgc tcacgcgcag catcgatccc gacggctcgc
     4981 tctccaggat gcgtgcgatc cgccgcgcgg cgctttccgt aacctgaacg ggggtggttt
     5041 ccatggtcat gccgcattcc gcctgagggt gctttccctc ttcgcctgca ataggtaagg
     5101 aaccggaccg gttgcttcaa cccgccgcat gaaattccgg gaacggatcg ttgcgttttt
     5161 accgcgaaag acagctttcc gcctttgctg cggtcgcgag ccgctcgcgc gggcgccttg
     5221 ttcgggaacc ggcatccccc acgcgcaccg aattccagcg tgatcgggac cgaatcgtcc
     5281 attccaccgc ctttcggcgc ctgaagcaca agacgcaggt gttcgtcgaa cacgagggcg
     5341 atcactaccg cacgcgtctg acccatacga tcgaggtggc gcagatcgcc cgcgccgtag
     5401 cccgagcgct ggcggtcgac gaggatctgg ccgaggcact ggcgctggcg cacgacctcg
     5461 gacaccctcc cttcggccac gccggcgaag acgtcctgga cgaatgtctc aatgattacg
     5521 gtgggttcga ccacaatgcc caggcgctgc ggatcgtcac gaggctggag cggcggtatg
     5581 ccgccttcaa cggactgaac ctgacctggg agacgctgga ggggttggtc aagcacaacg
     5641 ggccgttgct ggattcagcc ggaaagccgg tgaaacgtta tgaagaaaac ggcgttccgg
     5701 gggcgattct cgaattcgac gcggtttttc cgcttaacct gtccaccttc gccggggtgg
     5761 aagcacagat cgctgcgatc gccgacgata tcgcctacaa tgcccacgac atcgacgatg
     5821 gcctgcgggc cggactgttc gatctcgaat cgctcgtaga aacggatttc ctcggcgcac
     5881 tgatcgccga aatacgcggc gaatggccga tgctcgatac cgtgcgcacc gggcacgaac
     5941 tggtgcgccg ggtgatcacg cggttcgtcg aagatgtcat cgccgaagcc gagcggcggc
     6001 tcgtcgcgct cgcgccggag tcggtggagg cggtgcggca ggcggcggcg cccgtcgtcg
     6061 ccttctcgcc ggcaatggcc g
//
LOCUS       DAOMXN010000160         6060 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_6894_length_6060_cov_9.769026, whole genome shotgun sequence.
ACCESSION   DAOMXN010000160 DAOMXN010000000
VERSION     DAOMXN010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 6060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6060
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6894_length_6060_cov_9.769026"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="PK812_10615"
     CDS             complement(<1..392)
                     /locus_tag="PK812_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HRJ70050.1"
                     /translation="MQQLWTVLGPDGSKIVIWLVVGLGIAIAIAILIWLLMKLFGRNL
                     NMSGGGRGQPQRLGITGAFNVDRKGRKLVIVRRDNVEHLILIGGPNDVLVESNIVRAQ
                     AARSQAARSESAEMIEPAPPPPVVAPPAP"
     gene            587..967
                     /gene="flgB"
                     /locus_tag="PK812_10620"
     CDS             587..967
                     /gene="flgB"
                     /locus_tag="PK812_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015822945.1"
                     /note="with FlgF and C makes up the proximal portion of
                     the flagellar basal body rod; Bradyrhizobium has one thick
                     flagellum and several thin flagella; the protein in this
                     cluster is associated with the thick flagellum; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HRJ70051.1"
                     /translation="MSGRIPALSALKGRMQWLQARQAVLAGNVAHADTPGFRSRDLAP
                     ATAETGRLALTGTSPGHLGSAGRRAAEQSRAANAFETRPSGNGVSLEEEMLKLADTQV
                     EYQLAAGLYQRSIAYLRTSLGRRG"
     gene            970..1383
                     /gene="flgC"
                     /locus_tag="PK812_10625"
     CDS             970..1383
                     /gene="flgC"
                     /locus_tag="PK812_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HRJ70052.1"
                     /translation="MGDLARAIVSAASGLRAQAGRMRLVAENLANSDSTPAKPGEMPY
                     RRKVATFQNQVDRDSGAQTVTLGRVTRDGSAFRSRLDPGHPAADADGFVKSANVNSLI
                     EQADMREAQRSYEANLNVIGAARRMIARTLEILKA"
     gene            1395..1703
                     /locus_tag="PK812_10630"
     CDS             1395..1703
                     /locus_tag="PK812_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018261069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HRJ70053.1"
                     /translation="MTIDRFGLLRAASAYQAQGQATSIAPPARREAFADLLAQAVGGA
                     ADSGRKADAVRASVAGGQKPDYLEVVTAVAESEAALETLVAVRDRVVQAYEEIMRMPI
                     "
     gene            1703..1963
                     /locus_tag="PK812_10635"
     CDS             1703..1963
                     /locus_tag="PK812_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012319200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HRJ70054.1"
                     /translation="MAGLIDLARDGVLTFMKAGMPVMLAALLAGLIVSLGQALTQIQE
                     QTLVYVPKIVVALAALVFLLPYMGDALAGYMERVAFRIVTGG"
     gene            1932..2708
                     /gene="fliR"
                     /locus_tag="PK812_10640"
     CDS             1932..2708
                     /gene="fliR"
                     /locus_tag="PK812_10640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HRJ70055.1"
                     /translation="MSRSASSPGDRVTLALLPGFAEAFMLAFARIGTMIMLLPGLGER
                     AIPARMRLAVAVLLTLLLQPGLGRPEVAPLAGLLSEMAIGAVLGLSARLVLAAAETAG
                     SFAAQGVGLSFAQVVDPTQGQQGDALAAFFRMTGVLIVFATDLHHLAIAGIAGSYQAL
                     PPGAPVHIADGLTAFLSTLARAFAAAVMLAAPFLVFGLVFNLGTGLLGRLAPQIQVFF
                     LAVPLSVALGVLLLGRHLPSLWESLHAFVSATLTQILPLP"
     gene            2716..3771
                     /locus_tag="PK812_10645"
     CDS             2716..3771
                     /locus_tag="PK812_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HRJ70056.1"
                     /translation="MAEGQEQEDKTEEPSERRLEKAFERGEVPRSADLSAFVSVLSAG
                     ATAFATAAFVAGPVRDDLAAILANAAGASVVSAGAAGLRSAAMATAGPLLAGMAGAVC
                     AGLAMHRIVLTSEPLMPKLERISPAAGWKRLVGAENVVQFGKTALKVLVIIAVLAALW
                     RADGWRLGATGLLTDVNGLAEAMRALSGRVFWSVLAVFAVFAVLDAAYQRFAWRKRLR
                     MSHQEVKEEHKESEGSPEIKQRVRNLQRQRARQRMMAAVPKASVIIANPTHFAVALRY
                     EKGMAAPVCLAKGVDDLALRIRALAAEHRIPVVENPPLARALHATVKIDEEIPEQHYK
                     AVAEVIGHVMRLARQRR"
     gene            3907..>6060
                     /locus_tag="PK812_10650"
     CDS             3907..>6060
                     /locus_tag="PK812_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRJ70057.1"
                     /translation="MRDMPPTSIDRSDRQGSVVLVLILALFLVGAAVASYWFDDDKRS
                     LFILWFLAVLAALGLLALLLYAVGILQFSARAARNDLTKLIVDSTREGHLALDRDGKI
                     LYANGAYLQLCGGDASDPRPVERLFSGAPEVTEAVYRLAKAAREARPAVEEIRMAPAL
                     GGGEVGWYRVRVRQIDRDGSQAVLWTVADRTLERQRQENVFQELQHAIDYLDHAPAGF
                     LSAHPDGRIAYLNATLAGWLDLDIAQVGTNGLKLTDIVTGAGEALLSGPPGPPGTVST
                     ETIDLDLKRANGRTLPVRLLHRVAFAADGTRGPSRTLVLNRSEGEDAAEGLRAAQVRF
                     ARFFNATPLAIATIDKQGQVKQANAPFAKAFPTQAAKGGDALARSIVSAVAEADRPAL
                     QQALDAAREGRGDIPPVDVALAGEAPRSARIYVNAIEERDSQDEAAIVYALETTEQQK
                     LQEQFFQAQKMNAIGQLAGGVAHDFNNHLQSILGFTDLLLTNQKSTDPSFADLVQIKN
                     SANRAKSLVRQLLAFSRQQTLMPEEIHIGDRLSDLSNMLKRSIGPKVELDVRYSRDLW
                     PVHADPASFDNMIINLAVNARDAMPNGGKLTIRTANISTGEAAKIDNEQLKPADYALI
                     EVEDNGTGMAPEVLEKIFEPFFTTKEVGRGTGLGLSSAYGFVQQSGGVILCDSTQGQG
                     TTFRIFLPRFLPKAAAFDAAVAEAQRPAGETKAPAA"
BASE COUNT          993 a         1981 c         2046 g         1040 t
ORIGIN      
        1 ggcgccggtg gcgcgacgac cggaggcggg ggcgccggct cgatcatttc ggctgattcg
       61 gagcgcgcgg cctggctgcg cgcggcctgg gcgcgcacga tgttcgattc gaccagaaca
      121 tcgttgggtc cgccgatgag gataaggtgc tcgacattgt cgcggcgcac gatgaccagc
      181 tttcggcctt tgcggtccac gttgaacgcg cctgtgatgc cgagacgctg cggttgcccg
      241 cgtccgccgc cggacatgtt caggttgcgg ccgaacagct tcatcaacag ccagatcagg
      301 atcgcgatgg cgatggcgat acccagcccg accaccagcc agatcacgat cttcgacccg
      361 tccggcccca gaaccgtcca caattgctgc atgttccgtt actcttctac tcttcgatac
      421 gcgcgcagaa tgcccgggct atcgatacca cactcacggt gctgaccgcc atcaaggtta
      481 agacttagtt aacggcgacg ccaagcggcc ttaaccgttt cttaaccata aggtgggcaa
      541 atcttgccca acatgcaacc gtagattgtt taattaagga gtcgcaatgt cagggcgcat
      601 tcccgcgcta agcgctctca aagggcggat gcagtggctg caggcgcgtc aggccgtgct
      661 ggcaggaaac gtcgcccatg cggacacccc agggtttcgc tcgcgcgacc tggcgccggc
      721 caccgcggag acgggcaggc tggcgctgac cggcacatcg cccggccacc tgggttcggc
      781 cggtcgtcgc gccgccgaac agtccagggc cgccaatgcc ttcgagacac ggccgagcgg
      841 caatggcgtc agcctggagg aagagatgct caagcttgcg gacacgcaag tcgagtacca
      901 gctggcggcg gggctctatc agcggtccat cgcctatctg cgcacatcgc ttggacggag
      961 agggtgacca tgggtgatct ggcacgcgcg atcgtctcgg cggcgagcgg attgcgggcg
     1021 caggccggtc gcatgcggct ggttgccgag aatctcgcga attccgattc gacgccggcc
     1081 aagcccggtg agatgcccta ccgtcgaaag gtcgcgacct ttcagaacca ggttgacagg
     1141 gacagcggag cgcagaccgt cacgctcggt cgcgtcaccc gggacgggag tgcgtttcgc
     1201 agccgtctcg atcccgggca cccggccgcc gacgcggacg gcttcgtcaa gtcggccaat
     1261 gtgaattcgc tgatcgaaca ggcggacatg cgtgaggcgc agcgctccta cgaagccaac
     1321 ctgaatgtga tcggcgcggc gcggcgaatg atcgcgcgca cgctggaaat cctgaaagcc
     1381 tgagggggca agagatgacc atcgaccggt ttgggctcct gcgcgccgca agcgcctatc
     1441 aggcgcaagg acaagcgacg tctatcgcac cgccggcgag gcgggaggcc tttgcggacc
     1501 tgctcgcgca ggccgtgggc ggggcggccg atagcggccg caaggccgac gccgtgcgcg
     1561 cctcggtcgc aggggggcaa aagcccgact acctcgaggt ggtgacggct gtcgccgaga
     1621 gcgaggcggc tcttgagacg ctagtggcgg tgcgcgacag ggtcgtgcag gcctacgagg
     1681 aaatcatgcg gatgccgatc tgatggccgg gctgatcgac ctggcgcgcg acggcgtgct
     1741 caccttcatg aaggccggca tgccggtcat gctggcggcg cttctggccg ggctcatcgt
     1801 atccctcggg caggctctga cgcagattca ggaacagacg ctggtctatg ttccgaagat
     1861 cgtggtggcg ctggcggctc tggtgttcct cttgccctac atgggcgatg cgctggccgg
     1921 ctacatggag cgtgtcgcgt tccgcatcgt caccggggga tagggtgacg ctggcgctgc
     1981 tgcccggttt cgccgaagct ttcatgctgg ccttcgcccg tatcggcacc atgatcatgc
     2041 tgctgcccgg gctcggtgaa cgcgccattc cggcgcgcat gcgcctggcg gtggcggtgt
     2101 tgctgacgct gctgctgcaa ccggggctcg gccgaccgga agtcgcgccc ctggctggcc
     2161 tattgtccga aatggcgatc ggggcggttc tgggcctgtc ggcccgactg gtgcttgccg
     2221 cggcggagac ggcgggaagc ttcgccgccc agggcgttgg cctatccttc gcacaggtcg
     2281 tcgatccgac gcagggacag cagggcgatg ctctcgcagc cttcttccgg atgacaggcg
     2341 tgctgatcgt ctttgccacc gacctgcacc atctggcgat tgcgggaatc gcaggcagct
     2401 accaggcgtt gccgcccggt gcgcctgtcc acatcgcgga cgggctcacc gcgttcctgt
     2461 cgacccttgc ccgggccttt gcggcggcgg tgatgctggc tgcgccgttc cttgtcttcg
     2521 gactggtctt caacctcggt acgggcctgc tcgggcggct ggcgcctcag atccaggtct
     2581 tctttctggc ggtgccgctt tccgtcgcgc tgggtgtcct gctccttggc cgccatctgc
     2641 cctcgctctg ggaaagcctt cacgccttcg tgtcggcgac cctgacgcag attctgccac
     2701 tgccctgagg ctgccatggc ggagggacag gagcaggaag acaagaccga agaaccgtcc
     2761 gaacgacggc tggaaaaggc tttcgagcgc ggcgaagtgc cgcgttcggc tgacctttcg
     2821 gccttcgttt cggtgctttc cgcgggcgcg actgcctttg cgaccgcggc gttcgtggcc
     2881 ggcccggtgc gcgacgacct ggctgccata ctggcaaacg ctgcgggcgc ctcggtcgtc
     2941 agcgctggcg ctgccggtct gcggagtgct gcgatggcga ccgcaggtcc gctgctcgcg
     3001 ggcatggcag gagcggtctg cgcaggtctt gccatgcatc gcatcgttct gacgagcgaa
     3061 ccgctgatgc ccaagcttga acgcatcagt ccggccgctg gctggaaacg cctggtcggc
     3121 gccgagaatg tcgttcagtt cggcaagacc gccctcaagg tccttgtgat catcgcggtt
     3181 cttgccgcgc tgtggcgcgc ggacggctgg aggctcggtg cgacaggcct gctgacggac
     3241 gtcaatggcc ttgccgaggc gatgcgcgcc ttgtccgggc gggttttctg gagcgttctg
     3301 gcggtcttcg cggtgtttgc cgtgctcgac gcggcctatc agcgcttcgc ctggcgcaag
     3361 cgcctgcgca tgtcgcatca ggaggtcaag gaggagcaca aggaatcgga aggatcgccc
     3421 gaaatcaagc agcgggtgcg caacctgcaa cgccagcgcg cgcgccagcg catgatggcg
     3481 gccgtgccca aggcaagcgt gatcatcgcc aatcccacgc actttgccgt tgccctgcgc
     3541 tatgaaaagg gcatggccgc gcctgtctgt ctcgccaagg gcgttgacga cctcgcgctc
     3601 cggattcggg cgctggccgc cgagcatcgc attccggtcg tcgaaaaccc gccgctggcc
     3661 cgggcgttgc atgcgacggt caagatcgat gaagaaattc cggagcagca ctacaaggcc
     3721 gtcgccgaag tgatcggcca tgtcatgcgg ctggcaaggc agcgccgcta gacgcattcg
     3781 ccgcattcaa cggcgagcga atcgcagaaa agaccgctag agcttgtatc cagcgccccg
     3841 gggcttgcag ccgtcgcatt cacgcggcac agcggaatgg cccatcgcgg gtgcagggga
     3901 tgaaccatgc gcgacatgcc gccgacctcc atcgaccgta gtgaccgcca gggcagcgtg
     3961 gtgctggtgc tgatcctggc gttgttcctg gtcggtgcgg ctgtggcgag ctactggttc
     4021 gatgacgaca agcgctcgct gttcatcctg tggttcctgg cggttctggc ggcgctcggc
     4081 ctgctggcct tgctgctcta tgccgtcggc atcctgcagt tttccgcgcg ggcggcacgc
     4141 aacgatctca ccaagctgat cgtggactcg acccgggagg ggcatctggc gctcgaccgg
     4201 gacggcaaga ttctctatgc gaacggagcc tatctgcaat tgtgcggcgg cgacgccagc
     4261 gatccgcgcc cggtcgaaag actgttctcc ggcgcgccgg aagtgaccga ggcggtctac
     4321 cggctggcca aggcggcacg cgaggcgcgc ccggcggttg aggaaatccg catggctccc
     4381 gccctcggtg gcggcgaggt ggggtggtat cgggtccggg ttcgccagat cgaccgggac
     4441 ggctctcagg ccgtgctctg gaccgtcgcg gaccgcacgc tcgaacgcca gcggcaggaa
     4501 aacgtcttcc aggaattgca gcatgccatc gactacctcg atcacgcgcc ggccggcttc
     4561 ctttccgccc atccggatgg gcgcatcgcc tatctgaacg ccacgctcgc gggctggctc
     4621 gatctcgaca tcgcgcaggt cggaacgaac ggcctgaagc tgacggacat cgtcaccggg
     4681 gccggtgagg cgcttctgtc cggcccgccc ggcccgcccg gcacagtgtc gaccgaaacg
     4741 atcgatctcg atctcaagcg ggcgaacggg cgcaccctgc cggtgcgcct gctgcaccgc
     4801 gtcgccttcg cggcggacgg cacgcgcggc ccttcgcgca cgctcgtgct caaccgttcc
     4861 gagggcgagg acgcagccga aggcttgcgg gcggcgcagg tgcggtttgc ccgcttcttc
     4921 aacgcgacgc cgcttgccat cgccaccatc gacaagcagg ggcaggtgaa acaggcgaac
     4981 gcgcccttcg ccaaggcctt tccgacgcag gccgccaagg gcggggatgc gctggctcgc
     5041 tccatcgttt cggcggtggc ggaagccgac cggccggcgt tgcaacaggc gctggacgcg
     5101 gcccgcgagg gacggggcga cattccgccc gttgacgtgg cgcttgccgg cgaggcgccg
     5161 cgctcggcgc gcatctacgt caacgccatc gaggagcggg attcacagga cgaggcagcc
     5221 atcgtctacg cgctggaaac gacggaacag cagaagctgc aggagcagtt cttccaggcg
     5281 cagaagatga acgcgatcgg ccaactcgcc ggcggcgtgg cgcatgattt caacaaccac
     5341 ctgcagtcga tcctcgggtt cacggatctg ctgctgacca accagaagtc gaccgatccc
     5401 tcgttcgccg atcttgtgca gatcaagaac tcggccaatc gggcaaagtc tctggtgcgc
     5461 cagctgctcg ccttctcccg ccagcaaacg ctgatgccgg aggaaatcca catcggcgac
     5521 cggctctccg acctgtcgaa catgctcaag cgctccatcg gccccaaggt ggagcttgac
     5581 gtgcgctaca gccgcgacct gtggccggtg catgccgatc ccgcctcgtt cgacaacatg
     5641 atcatcaacc tggcggtgaa cgcgcgcgac gccatgccga acggcggcaa gctgacgatc
     5701 cgcaccgcca acatctccac cggcgaggcg gcgaagatcg acaacgagca gctcaagccg
     5761 gcggactatg cgctgatcga ggtcgaagac aacggcacgg gcatggcacc ggaagtgctg
     5821 gagaagattt tcgaaccctt cttcaccacg aaggaagtcg ggcgcggcac gggcctcggc
     5881 ctgtcctcgg cctatggctt cgtgcagcag tcgggcggcg tcatcctgtg cgactcgaca
     5941 caggggcagg gcacgacctt ccgcatcttc ctgccgcgct tcctgcccaa ggccgccgcc
     6001 ttcgacgcgg ccgtggcgga agcccagcgc ccggccggcg agaccaaggc ccccgccgcc
//
LOCUS       DAOMXN010000161         6017 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_6966_length_6017_cov_9.770044, whole genome shotgun sequence.
ACCESSION   DAOMXN010000161 DAOMXN010000000
VERSION     DAOMXN010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 6017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6017
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6966_length_6017_cov_9.770044"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="PK812_10655"
     CDS             <1..195
                     /locus_tag="PK812_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HRJ70058.1"
                     /translation="QGLGTCAQGYWARYPQTLKRILDHDDDHMVCSGMALGWPDRAAP
                     INTLRTRRDPLEAWGEMKGF"
     gene            complement(213..608)
                     /locus_tag="PK812_10660"
     CDS             complement(213..608)
                     /locus_tag="PK812_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70059.1"
                     /translation="MLAALTSDDPTRTARLRRVLAVDALTAVGFGLAASLAAPGLATI
                     LAIPESLVRIAGLISLAATVLLAAAALSPAPSRTLVKVIVAGNALWVAASIVLLATGF
                     GNASGMAVIAAQGLFVLVLTTMEHRLAPS"
     gene            722..1501
                     /locus_tag="PK812_10665"
     CDS             722..1501
                     /locus_tag="PK812_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011652354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRJ70060.1"
                     /translation="MERIRHAGAMLREWRGIRRLSQMELALRSGISSRHLSYVETGKS
                     TPGRAVVLELAEQLDMPLRVRNQTLLMAGHAPCFPDQTLDDPSRKPFREMIERVLKGH
                     EPYPALAVDRHWNLVAANAALRPLLELCAPFLLQPPVNVLRLALHPEGLARAIVNLGE
                     WHALITTRLRHQLRQAPDPTLMALLRELSAFPAPPAPTPPQTDTVDLAVPITLRTPEG
                     LLNLIGISMVFGSPWDVTVSEIAIEAFLPADESTAQLLVRS"
     gene            complement(1508..1942)
                     /locus_tag="PK812_10670"
     CDS             complement(1508..1942)
                     /locus_tag="PK812_10670"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HRJ70061.1"
                     /translation="MMFRTLGLAALLPLAACNASQMDFSGALSRSFSFANASLERSNY
                     ASANLAGANFAGADIDRANFSRANLANANLSSVSASETLLVGTDMQGADLTNARFTNA
                     SLQGANLKGATIDYAVFDGANLTGATWVDGKVCGQNSIGECR"
     gene            complement(1939..2883)
                     /locus_tag="PK812_10675"
     CDS             complement(1939..2883)
                     /locus_tag="PK812_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012252003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRJ70062.1"
                     /translation="MKNHADDPVAGDRFSCEAIEQVIVPRLAALGPAMPVRRALPAAG
                     RMMVGPFIFFDQFGPAEFLLGQGMDVNPHPHIGLSTVTWLFEGEITHRDSLGVTQPIR
                     PGEMNLMTAGRGIVHSERSPYSERVGGKRLFGIQLWAALPASHEEQAPQFVHYGADDL
                     PRIAGDGKRVRLIMGSLYGEDSPVAFPHDAFYAEAVLAPGAVLPLDADYDERAIYIVS
                     GSVDIAGDRFDAGRLIVFKPGDRMSVLALSNARLMLLGGAPMDGPRHIWWNFVSSRRE
                     RIEQAKADWAAGRFDAVPGEELGGTARVAHGAAGKAEQ"
     gene            complement(2966..3217)
                     /locus_tag="PK812_10680"
     CDS             complement(2966..3217)
                     /locus_tag="PK812_10680"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004110005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HRJ70063.1"
                     /translation="MTTNAKSDIAALPFEKALAELEQIVRKLEQGEVALEESIALYER
                     GELLKKRCEALLAAAEARIEKITLGADGSAKGAEPFPQG"
     gene            complement(3499..4122)
                     /locus_tag="PK812_10685"
     CDS             complement(3499..4122)
                     /locus_tag="PK812_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HRJ70064.1"
                     /translation="MLDFKLKKPDFKLKKPDIKLDDEAKFLKSWLNNPIKTGAVSPSG
                     KALAAVMAEAVDPTLAGPVIELGPGTGPVTEALIDNGIAENRLVLVEYNAEFCALLRK
                     RFPEARVVQGDAYALKDTLGNVLNEPAAAIVSSLPLVTKPDAECLKLVFQGFDLMLPG
                     APFVQFTYGPVSPVPVKGRGFKATVSKRVWLNLPPARVWTYRRFKVG"
     gene            complement(4356..5483)
                     /gene="dnaJ"
                     /locus_tag="PK812_10690"
     CDS             complement(4356..5483)
                     /gene="dnaJ"
                     /locus_tag="PK812_10690"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010684984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HRJ70065.1"
                     /translation="MSKRDYYEVLGVPKNADEAALKSAFRKLAMQHHPDKNPGDKAAE
                     AKFKELNEAYQVLSDGQKRAAYDRFGHQAFEQGGGGPGGFGTDFGASMSDIFENIFGE
                     VFGGGRRSAGGRERGADLRYNLEITLEEAFVGKAATLKVPTSVTCEACTGTGAKPGTK
                     PKTCTTCAGHGRVRATQGFFAIERTCPTCHGRGQIIDDPCRSCAGAGRVMKERTLQVN
                     VPPGVEDGTRIRLSGEGEAGARGGPAGDLYVFLSVKPHSLYQRDGADLFARVPISMVA
                     AALGTEISVPTLDGTDAKVRIPEGTQSGKQFRVKAKGMPVLRTKDRGDLFIQCVVETP
                     QNLTRRQKELLREFQEGSSKETQPEAEGFFARMKKDFFGGA"
     gene            complement(5487..>6017)
                     /locus_tag="PK812_10695"
     CDS             complement(5487..>6017)
                     /locus_tag="PK812_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70066.1"
                     /translation="EDVIDADFKEVKKDDKKSGRDPPARSERPGGSRLRLRLAVKAES
                     VRATATAPRPVEERMQAALAVDSFERHRLNYGCFLTGVFRNTGDVVIPAGAVRLASMN
                     GDLPGHFASIVNFWPIVPKESLTLSLVWPFPEGHPDRVLLVDYVALDGQVIAPLDTEV
                     DFSLILGTSTLRPAER"
BASE COUNT         1080 a         2018 c         1850 g         1069 t
ORIGIN      
        1 caggggctcg gtacctgcgc gcaaggctat tgggcgcgct atccgcagac gctcaagcgc
       61 atccttgacc acgacgacga ccacatggtg tgttccggca tggcgctggg ctggccggac
      121 cgggccgcgc cgatcaacac cctgcgcacc cggcgcgatc ccctcgaagc ctggggcgag
      181 atgaaggggt tctgatgcgg ccggacactg cgctagctgg gcgcaagccg gtgttccatt
      241 gtggtcagta cgagaacgaa cagaccctgc gccgcgatga cggccattcc ggatgcgttg
      301 ccgaagccgg tggcgagcag gacgatgctg gccgcgaccc aaagggcatt gccggccacg
      361 atcaccttca ccagtgtccg cgacggtgcc gggcttagcg ccgcggccgc caggagaacc
      421 gttgccgcca gcgaaatcag gcctgcgatc cgcaccaggg attcgggaat ggccagaatg
      481 gtcgcaaggc ccggcgcggc caggctcgcc gccagaccga agccaacggc cgtgagtgcg
      541 tcaacggcca gaacacgccg caatcttgcc gttcgggtcg ggtcatcgga agtgagtgcg
      601 gcaagcatgg acggtgctcc tgtgtctgga tgtgcggccg ctacgtttcc agcttcaggg
      661 ggcaaggtcc atgacctcag aggtaatgga atcgcggccg ggatcgggta gctttccatt
      721 catggaacgg attcgtcatg ctggagcgat gctgcgggag tggcgcggca ttcgccgcct
      781 gagccagatg gaactggcgc tcaggtcagg catctcatcg cgccacctca gctatgtcga
      841 aaccggaaag tcgacgcccg ggcgggccgt ggttctggaa ctcgccgagc aactcgacat
      901 gccgctgcgt gtgcgcaacc aaacgctcct gatggccggt catgcgccct gctttcccga
      961 tcagaccctt gacgatccca gccgcaagcc attcagggag atgatcgagc gcgtgctgaa
     1021 agggcatgag ccctatccgg cgcttgccgt cgaccggcac tggaaccttg tggccgcaaa
     1081 tgccgctctc aggcccctgc tggagctttg tgcgccattt ctgctgcaac cgccggtcaa
     1141 tgtcctccgg ctggccttgc atccggaagg cttggcgcgg gccatcgtca acctgggcga
     1201 atggcatgcc ctgatcacga cgcgcctgcg tcaccagctc cgacaggcgc cggacccaac
     1261 gctgatggca ctgcttcggg aactctctgc gttcccggcg ccgccagcgc cgacacctcc
     1321 acaaaccgac acagtcgacc tcgcagtgcc gataaccttg cggacgccgg agggactcct
     1381 caatctcatc ggcatttcca tggtgttcgg ttcaccctgg gatgtcaccg tttccgaaat
     1441 tgccatcgag gcctttctgc ccgccgacga gtcgaccgcg cagctgctcg tccgatcctg
     1501 atggcgttca ccggcactcg ccaatggaat tctggccgca gaccttgcca tccacccagg
     1561 tcgcaccggt gaggttcgcc ccatcgaaaa cggcgtagtc gatcgtcgcg cccttgagat
     1621 tggcgccctg aagactggca ttcgtgaatc gtgcgtttgt cagatccgca ccctgcatgt
     1681 cggtgccgac cagcagggtt tcgcttgccg agacactcga caggttggcg ttggcgagat
     1741 tggcccggga gaaattggcg cgatcaatgt ccgcgcccgc aaaattcgcg ccggcgagat
     1801 tggccgaggc atagttcgag cgttccagac tggcattggc gaagctgaac gagcgcgaca
     1861 aggcgccgct gaaatccatc tgcgaggcgt tgcaggccgc cagcggcaat agagcagcga
     1921 ggccaagagt tcgaaacatc attgctctgc ctttccggcg gcgccatgcg ccacacgcgc
     1981 cgtcccgccc agttcctcgc ccggaacggc gtcaaaccgg ccggcagccc agtcggcctt
     2041 ggcctgctcg atccgctcgc gccgggacga cacgaagttc caccagatgt gccggggacc
     2101 atccatcggt gcgccgccga gcagcatcaa ccgagcattg gacagggcga gcacggacat
     2161 ccggtctccc ggcttgaaca cgatcagccg gcccgcgtca aaccgatccc cggcaatgtc
     2221 gacggacccc gaaacaatgt agatcgcgcg ctcgtcgtaa tccgcgtcga gaggcagcac
     2281 ggcgccgggc gccagcaccg cctcggcata gaaggcatca tgcgggaagg cgacgggact
     2341 atcctcgcca taaaggctgc ccatgatcaa ccgcacgcgc tttccatccc cggcgatgcg
     2401 cggcaagtcg tctgcgccat agtggacgaa ctgcggcgcc tgttcctcgt ggctcgccgg
     2461 caaggcagcc catagctgga tgccgaaaag ccgtttgccg ccgacgcgct ccgaatacgg
     2521 cgatcgctcg gaatgcacga ttccgcggcc ggccgtcata aggttcatct cgcctggtcg
     2581 gataggctgg gtcaccccga gcgaatcgcg atgggttatc tcgccctcga acagccaggt
     2641 gaccgtcgac aggccgatat gcggatgcgg attcacgtcc atgccctggc ccagcaggaa
     2701 ttccgccggc ccgaactgat cgaagaaaat gaacggcccc accatcatgc ggccggccgc
     2761 cggaagggca cggcgcaccg gcatcgcagg cccgagggcc gcgagacgag gcacgatcac
     2821 ctgttcgatc gcctcgcagg aaaaacgatc tccggcaacg ggatcatcgg cgtggttttt
     2881 cataatccaa tccggatggg cacgccgcgc cgcgtgcagc cctgaatgtc tgcctgccga
     2941 ttgcggagct ttcgtgaccg tggcgtcaac cctgggggaa tggttcggcc cctttggccg
     3001 agccgtccgc gccgagcgtg atcttttcga tccgcgcttc cgccgcggcg agcagagcct
     3061 cgcagcgctt cttcagcagt tcaccgcgct catagagcgc aatcgattcc tccagcgcga
     3121 cctcgccctg ctccagtttg cggacgatct gctcgagttc ggcaagcgct ttctcaaagg
     3181 gcagagcagc gatgtcggat tttgcgttgg tggtcatggc aagagcttgt agcggaacag
     3241 aattggactc gccatatcca tcgacgttgc caacaggatt gccgatcccg ccgatgcctg
     3301 accgccacac ccccaatccc cgcgcctcga tcggcgccgc catcgtggtc gccctgctgt
     3361 tggcggccgg ggtctatgtc ttctggcgcc tcgaccagag ccagcgggcg caggcctgcc
     3421 tggagtccgg ggggcgtaaa tgtgccatca tcgacagtcc gccggcgcga tagcggccct
     3481 ggcgggttcg gatccgggct atccgacctt gaaccgccgg taggtccaga cccgggccgg
     3541 cggcagattc agccagaccc gcttggatac cgttgccttg aaaccgcgcc ccttcaccgg
     3601 caccggcgag acagggccgt aggtgaactg cacgaacgga gcgccgggca gcatcaggtc
     3661 gaagccctgg aaaacaagct tcaggcactc ggcgtcgggc ttggtgacga gcggcaacga
     3721 cgagacgatg gcagcggccg gctcattcag cacgttgccg agcgtgtcct tgagggcgta
     3781 ggcatcgccc tgaacgaccc tcgcctcggg aaagcgcttg cgcaggagcg cgcagaactc
     3841 agcattgtac tcgaccagaa ccagccggtt ttcggcgatg ccgttgtcga tcagcgcctc
     3901 ggtgaccggt ccggtgcccg ggccgagttc gatcaccggg cccgccaggg taggatcaac
     3961 cgcctccgcc atcaccgccg ccagcgcctt gcccgaaggc gagacagcgc cggttttgat
     4021 cgggttgttc agccaggact ttaagaactt cgcctcgtcg tcgagcttga tgtccggctt
     4081 cttcagcttg aagtccggtt tcttcagctt gaaatcgagc atcggtccgc gcagcgagac
     4141 cttgagttcg ccctcttcaa ccttgacgtt gaagtccggc ttgatgaagc ggaacggctt
     4201 gttcgaatgt gcgttcacgt ttccgtccgt ggctccgtgc gtccaggcac ccgtgcctgt
     4261 cgcacctgcg tcatcaatca tcgataactt tggctatttt acagcgcgtt gcggaaagcg
     4321 caaacgcgag cgcctgacgg cacgcccagg cctcgtcagg cgccgccgaa gaagtccttc
     4381 ttcatgcgcg cgaaaaaccc ctccgcctcc ggctgggttt ccttcgagga gccttcctga
     4441 aactcgcgca gcagttcctt ctgccggcgc gtcaggttct gcggggtttc gaccacgcac
     4501 tggatgaaca ggtcgccgcg atccttggtc ctgagaaccg gcatgccctt ggccttgacg
     4561 cgaaactgct tgcccgattg cgtgccttcc ggaatcctga ccttcgcatc ggtgccgtcg
     4621 agcgtcggta cggatatttc cgtccccagc gccgccgcga ccatcgagat cggcacccgc
     4681 gcgaagagat cggcaccgtc gcgctgatac aggctgtgcg gcttgaccga gaggaagaca
     4741 tagagatcgc ctgccggccc gccgcgcgca cccgcctcac cctcgcccga aaggcggatg
     4801 cgtgtgccat cctcaacccc gggtggcacg ttcacctgca aggtccgctc cttcatcacg
     4861 cgaccggcgc cggcacagga ccggcaggga tcatcgatga tctgaccccg cccatggcag
     4921 gtcggacacg tccgctcgat cgcgaagaag ccctgggtcg cccgcacccg gccatgaccg
     4981 gcgcaggtgg tgcaggtctt cggcttggtg ccgggcttgg cgccggttcc cgtgcaggcc
     5041 tcgcaggtga cggaggtcgg caccttgagc gtcgcggcct tgccgacaaa cgcttcctcg
     5101 agcgtgattt cgagattgta gcgcaggtcc gctccgcgct cgcgcccgcc cgcggaacgc
     5161 cggccgccgc cgaacacctc gccgaagata ttctcgaaga tgtcggacat cgaggcgccg
     5221 aaatcggtgc cgaagccgcc cggcccgccg cctccctgct cgaaggcctg atggccgaac
     5281 cggtcatagg ccgcgcgctt ctggccgtcg gaaagcacct gataggcctc gttcaattcc
     5341 ttgaacttgg cttcggctgc cttgtcgccc ggattcttgt ccggatgatg ctgcatcgcg
     5401 agcttgcgga aggcgctttt cagcgccgcc tcgtcggcat ttttcggaac gcctagaacc
     5461 tcgtaatagt cgcgcttgga cattgctcag cgctccgccg gccgcagggt agacgttccc
     5521 agaatcaggc tgaaatccac ctcggtatcc agaggagcaa tcacctgtcc atcaagcgcc
     5581 acgtagtcaa caagcaggac gcgatccggg tgcccttccg gaaaaggcca gacgagcgac
     5641 agcgtcaggg actccttcgg cacgatcggc caaaaattca cgatggatgc aaaatgcccg
     5701 ggcagatccc cattcatcga cgccagccgg acagcgccgg ccggaatgac gacatcgccc
     5761 gtgttccgga aaacgccggt gaggaaacag ccgtagttca gccgatgcct ttcgaacgaa
     5821 tcaaccgcca aggcagcctg cattctttcc tccacaggcc gcggcgcagt cgccgtcgcc
     5881 cgaacgctct ccgccttcac ggccagtcgc agtcgtaacc ggctcccgcc cggcctttcg
     5941 gaccgggcgg gaggatcacg tcccgatttc ttgtcgtcct tcttcacttc cttgaagtcg
     6001 gcatcgatca cgtcctc
//
LOCUS       DAOMXN010000162         5905 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_7133_length_5905_cov_11.278974, whole genome shotgun sequence.
ACCESSION   DAOMXN010000162 DAOMXN010000000
VERSION     DAOMXN010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 5905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5905
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7133_length_5905_cov_11.278974"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1798)
                     /gene="sulP"
                     /locus_tag="PK812_10700"
     CDS             complement(<1..1798)
                     /gene="sulP"
                     /locus_tag="PK812_10700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HRJ70067.1"
                     /translation="MSLSRPAPAAGTNRLTRALPFLTWMRRTNGKTVRADALAGLTGA
                     IVVLPQGVAFATIAGMPPEYGLYTGIVPAIIAALFGSSWHLVSGPTTAASVVLFSALS
                     GFAEPGSAHYVQLAITLALMVGLIQLALGLAKLGTIVNFISHSVVIGFTAGAGVLIIL
                     NQVRSFFGLNIPRGADPGEIILYVLTHPAEIAWPSVVVGAVTLGTGLIARRRWPKFPY
                     MITAILVGSLFAFALAQSGAPKIATVGALPASLPPLSMPSLNPVIWVQLTPTALAVTL
                     FALTEAISISRALAVKSEQHINSNQEFIGQGLSNVAGSFFSSYVATGSFNRSGVNYEA
                     GAQTPLASIISALLLATLVLLVAPVLAYMPHAAMAGVLFIIGWGLIDFHHAEQIWRTS
                     KPDAAVMAATFLATLLLDLEMAIFVGVTVSLMLYLHRTSNPSLRPRVPDPFSSGRKFT
                     DVRSDLPECPQARVARLDGSLYFGSVNSFIETVLEYEATDPDRKFLLIVMTGVNFIDT
                     AGAETLARVARRYRDRGGQLYLIRLKEGPRATLEQGGYLDVIGKDNIFWSKTDALRAL
                     YRRLDYDRCRACTLNVFIECARKGMQEPTDDKP"
     gene            complement(2199..2969)
                     /locus_tag="PK812_10705"
     CDS             complement(2199..2969)
                     /locus_tag="PK812_10705"
                     /inference="COORDINATES: protein motif:HMM:NF019032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HRJ70068.1"
                     /translation="MRAYPEKTSKLARWSQRLAVFGAATCLVGVALGRFAVFPLLQSL
                     AVFGAGQALCALALVLALAAYAEIWRNGARGLPKANLGLLVALSALAFPGYLVVQAYS
                     LPRINDISTDLRDPPVFSRARSAVEARGGHVPPDEPPQTREAQRLAYRDVAPVILDVS
                     PEEAYKLALEAAQQLKWQIVDRSPPSVRVRTGRLDAIDRSLILRFPDDITIRIRGLGN
                     DTRVDVRSVSRVGRHDFGVNAARIRRYTDLLLQLSKGI"
     gene            3095..3562
                     /locus_tag="PK812_10710"
     CDS             3095..3562
                     /locus_tag="PK812_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HRJ70069.1"
                     /translation="MSPASPADVARVWFRFIRLSQALSGVASSRLRQAGLSVPQFDVL
                     STLTEREGCTQRELAERLYVTKGNVSGLIDRLVEAGYVERRAIPGDRRSHALYLTAAG
                     RTKAEHGMALHQAIMRETLGALSAQDVLQLDQTLVTWRDVVRKVMDGEDRART"
     gene            3668..3895
                     /locus_tag="PK812_10715"
     CDS             3668..3895
                     /locus_tag="PK812_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70070.1"
                     /translation="MTPRFEQIGETLRPQRPGVGRDARAQLAHLYREIGISAVAAALH
                     AQGLASVEEREAVKRASSEIPPMLRKENLAA"
     gene            3930..4835
                     /locus_tag="PK812_10720"
     CDS             3930..4835
                     /locus_tag="PK812_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRJ70071.1"
                     /translation="MNRRTILKALAGAFLGAFGLGGYAFAVEPGWRFVTTRYRVRPKG
                     WPTGARLKIAAIADVHASEPQMGLKRIADVVRAANAAEPDLIVLLGDYGVGQRIYARP
                     VAHGELAQVLSGLKAPLGVFGILGNHDYWGDSLRNWPYWRDKVNDLAEPYRRLLRESG
                     VALLENDVRRIAFGAHAFWLIGTGSLIALPLGHARFESFADIDGQLPKLTDDAPAILL
                     AHEPDLFPRVPERIGLTLSGHTHGGQFRIFGYSPITPSKFGNRYAYGHVVEGSRNLIV
                     SAGLGTSIMPMRFGVPPELVIVEVG"
     gene            complement(4893..5489)
                     /locus_tag="PK812_10725"
     CDS             complement(4893..5489)
                     /locus_tag="PK812_10725"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRJ70072.1"
                     /translation="MTALKIAAAAMVALVLAGCKYETFSDPQLSGRDLELMKLAPPMP
                     PVDPMRARYRITNPTGEKQPGTIVVESNAKFLYLIEEGGTALRYPIAVGQDHYQWIGE
                     AKVGRKAEWPSWTPGSEARSLMPGLPATVPGGAQNPLGARALYLYDGGKDTLYRIHGT
                     NDPESIGHNVSLGCIRMHNIDAIDLYNRVKEGARVIVR"
     gene            5702..>5905
                     /locus_tag="PK812_10730"
     CDS             5702..>5905
                     /locus_tag="PK812_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HRJ70073.1"
                     /translation="MATLLIHDTLFQKHVMPQGHPERPARLEAIDKALSLPLFDALKR
                     EGARAGDPVLAELVHPAAHVRAIG"
BASE COUNT         1004 a         1886 c         2025 g          990 t
ORIGIN      
        1 ggggcttgtc gtccgtcggt tcctgcatgc ccttgcgggc gcattcgatg aagacgttca
       61 gtgtgcaggc gcggcacctg tcgtagtcga ggcggcgata gagcgccctc aacgcatcgg
      121 ttttcgacca gaagatgttg tccttgccga tgacatcgag atagccgccc tgttccagcg
      181 tggccctcgg gccttccttg agccggatta ggtagagctg accgccgcga tcgcggtagc
      241 ggcgggcgac gcgcgccagc gtttccgcgc ccgcggtatc gatgaagttc acgccggtca
      301 tgacgatcag caggaacttg cggtccgggt cggtcgcctc gtactcgagc acggtctcga
      361 tgaaggagtt caccgagccg aagtagagcg agccatcgag gcgcgcgacc cgcgcctggg
      421 ggcactccgg caggtcggac cggacgtcgg tgaactttcg gccgctgctg aacgggtcgg
      481 gcacgcgcgg acgcagggac gggttggacg tcctgtgcag atagagcatc agcgacaccg
      541 tgacgccgac gaagatcgcc atttcgaggt cgagcaacag ggtcgcgagg aaggttgcgg
      601 ccatcacggc ggcatccggc ttgctcgtgc gccagatctg ctcggcatgg tggaagtcga
      661 tcagtcccca gccgatgatg aacagcacgc cggccatggc ggcgtgcggc atgtaggcga
      721 ggaccggcgc gaccagcaac acgagcgtgg caagcagcag cgcggagatg atggacgcca
      781 ggggcgtttg cgcgccagcc tcgtagttga cgccgctgcg gttgaacgag ccggtcgcga
      841 cgtagctgga aaagaagctg ccggcgacgt tcgacaggcc ctggccgatg aactcctggt
      901 tgctgttgat gtgctgttcg cttttcaccg cgagtgcacg cgagatcgag atcgcctcgg
      961 tcagcgcgaa cagcgtgacg gcgagcgcag tcggcgtgag ctgaacccag atgaccggat
     1021 tgaggctcgg catcgacagc ggcggaaggc tcgcgggcag ggcgccgacc gtcgcgatct
     1081 tcggcgcgcc ggactgggcg agggcgaagg cgaacagact gccgacgagg atggccgtga
     1141 tcatgtaggg gaatttcggc cagcgccgcc gcgcgatgag tccggtgccc agcgtcacgg
     1201 cgccgacgac gactgacggc caggcgatct cggccggatg cgtcaggaca tagaggatga
     1261 tctcgcccgg atcggcgccg cgcgggatgt tgagtccgaa gaacgagcgc acctggttga
     1321 ggatgatcag cacgcccgcg ccggcggtga agccgatcac cacggaatgg gagatgaagt
     1381 tgacgatggt gccgagcttg gccaggccca gcgccagctg gatcaggccg accatcagcg
     1441 ccagcgtgat ggcaagctgc acataatgcg cgctgcccgg ttccgcgaac ccgctcagcg
     1501 ccgaaaacag gaccaccgag gcggcggtcg tcgggccgga gaccaggtgc caggatgatc
     1561 cgaacagcgc ggcgatgatg gccggcacga tgccggtgta cagcccgtat tccggcggca
     1621 tgcctgcgat ggtggcgaag gcgacgccct gcggcaggac cacgatcgcg ccggtcagtc
     1681 ctgccagggc gtcagcccgc acggtcttgc cgtttgtccg ccgcatccag gtcaggaagg
     1741 gcagggcacg cgtgagccgg ttcgtgcctg cggcgggtgc gggtctggaa aggctcatcg
     1801 tgggtccgtt gcgaggtggg ccgctgcgaa gcgcccgcga gccatcaggt gccgactctg
     1861 gcggggtgct gcggattcga gaatccgaca ataccctcca ggattggccc aaagaagggt
     1921 ttgttctttc cccccgcgct gcgctgcgga aaaatttccg cgtacgcgat ccggccgcct
     1981 gccgcgttcg gggaattggc gctgcattcc ggccgggcgg accggtgctc gcgaaagcac
     2041 agacgatgcc ggaggctggc gtgctcaacc acgttgcgtt tcctcgctgt atcccgccgg
     2101 ctggtcgaga cgttgtcata taagagacta tgaatatacg aatatatgtc ccgggcaagc
     2161 gcaacttgct cgccatggcg tgaggcgcgg tgcacggctc agatgccctt gctcagttgc
     2221 agcagcaggt ccgtatagcg ccggatgcgg gccgcgttga cgccgaagtc gtgtcgtccg
     2281 acgcgcgaga cggaacgcac gtcgacccgc gtgtcgtttc ccagcccgcg tatgcggatg
     2341 gtgatgtcgt cgggaaaccg caggatgagg ctgcggtcga tggcatcaag acgcccggtg
     2401 cggacccgaa ccgaaggcgg tgagcggtcg acgatctgcc acttcagctg ctgcgccgct
     2461 tccagcgcca gcttgtaggc ttcttccggc gagacgtcga ggatgaccgg cgcgacgtcg
     2521 cggtaggcca gccgctgcgc ttcccgggtc tgcggtggct cgtccggtgg cacatggccg
     2581 ccgcgtgcct cgaccgctga gcgcgcgcgg gagaacaccg gcgggtcacg cagatcggtc
     2641 gagatatcgt tgatccgggg caggctgtag gcctgcacga ccaggtatcc cggaaaggcc
     2701 agcgcgctta acgccacgag cagcccgaga ttggccttgg gcaggccccg ggcgccgttg
     2761 cgccagatct cggcgtaggc tgccagcgcc aacaccagcg ccagcgcgca gagcgcctgc
     2821 cccgcgccga acacggcgag cgactgcaac agcggaaaga cggcgaagcg gccgagcgcg
     2881 acgccgacga ggcatgtggc cgcaccgaac acggccaggc gctggctcca ccgggccagt
     2941 ttcgaggttt tctcaggata ggcgcgcatg acgggccgaa aaatgcagca tgaccatgca
     3001 tggctgctcg caccggtccg cgtcaactcg aacctgcagg ttgcggaaat cacgcggaac
     3061 tggttagagg aaaacttggc tttcagttgg tccgatgtcc ccagcttctc ccgccgacgt
     3121 cgcgcgcgtc tggttccgct tcatccggct tagccaggcg ctgagcggcg tcgcctcgtc
     3181 gcggctgcgt caggccggcc tgtccgtgcc gcagttcgac gtgctctcga cgctcaccga
     3241 gcgcgagggc tgcacgcagc gcgaactcgc cgagcgcctt tatgtcacca agggaaacgt
     3301 ctccggcttg atcgatcgtc tggtggaggc gggatatgtc gagcggcgtg cgattccagg
     3361 ggatcgccgc tcgcatgccc tctatctgac cgccgccggc cggaccaagg cggagcacgg
     3421 catggcgttg caccaggcca tcatgcgcga gacgctgggt gccctttcgg cgcaggacgt
     3481 gctgcagctt gatcagacgc ttgtcacctg gcgcgacgtg gtgcgcaagg tgatggatgg
     3541 cgaggaccgc gcgcgcacct gaggcggagc ggtgccggca tttaccgaac agtaaccgcg
     3601 ccggtcgaag gatgagccat gctcgccggc cgcgatcccg cgcccgcctg gaaattcgga
     3661 aggtcccatg actccgcgct ttgaacagat tggcgaaaca ctcagaccgc agcgcccggg
     3721 tgttgggcgg gatgcgcgcg cccagctggc gcacctgtat cgcgagatcg gcatctcggc
     3781 cgtcgcggcc gccctccacg cgcagggact ggcctcggtc gaggagcgcg aggccgtgaa
     3841 gcgcgccagc agcgagatcc cgccgatgtt gcgcaaggag aacctagctg cctgagcaac
     3901 ggcgcattcc tggggcaggg ccgccaccga tgaaccgtcg cacgattctc aaggcgctcg
     3961 ccggcgcttt cctgggcgcc ttcggccttg gtggctatgc cttcgcggtc gaaccgggct
     4021 ggcgtttcgt caccacgcgc tatcgcgtgc ggccgaaggg ctggcccacc ggggcgcggc
     4081 tgaaaatcgc ggcgatcgcc gacgtccacg cctccgaacc gcagatgggg ctgaagcgca
     4141 tcgccgacgt cgtgcgcgcc gccaacgccg ccgagccgga cctgatcgtc ctgctgggcg
     4201 actacggtgt cggtcagcgg atctacgcgc gccctgttgc ccatggcgag ctcgcgcagg
     4261 tcctgtcggg gttgaaggcc cctctcggcg tattcggcat cctcggcaac cacgactact
     4321 ggggggacag cctgcgcaac tggccctact ggcgcgacaa ggtgaacgat ctcgccgaac
     4381 cgtatcgacg gctgctgcgc gagtcaggcg tcgccctttt ggagaatgac gtccgccgca
     4441 ttgcgttcgg cgcgcatgcg ttctggctca tcggcaccgg ttcgctgatt gccctgccgc
     4501 tgggccacgc ccgcttcgag agcttcgccg atatcgacgg ccagttgccg aaactcaccg
     4561 acgacgcgcc ggcgatcctg ttggcgcatg agccggatct tttcccgcgc gtaccggagc
     4621 ggatcggcct gaccctgtcc gggcacaccc atggcggtca gttccggatc ttcggctact
     4681 cgccgataac cccctctaag ttcggcaaca ggtacgccta cggccatgtg gtcgaaggct
     4741 cacgcaatct gattgtctcg gccgggctgg gcacctcgat catgccgatg cgcttcggcg
     4801 ttccgccgga actggtcatc gtcgaggtcg gctgaatcga aaagggccgg cgtggaccgg
     4861 ccctttcttg tctttgcctc aggtcaggcg gatcagcgca cgatgacgcg cgcgccctct
     4921 ttcacacgat tgtagaggtc gatcgcgtcg atgttgtgca tgcggatgca gccgagcgac
     4981 acgttgtggc cgatcgactc cggatcgttg gtgccatgga tgcggtacag cgtgtccttg
     5041 ccgccgtcgt agagatacag ggcgcgggcg ccgagcgggt tctgggcgcc gcccggaacc
     5101 gtggccggca ggcccggcat cagcgaacgc gcttcgctgc cgggcgtcca gctcggccat
     5161 tccgccttgc ggccgacctt ggcctcgcca atccactgat agtggtcctg gccgacggcg
     5221 atcggatagc gaagggccgt gccgccttcc tcgatgaggt agaggaactt ggcgttggat
     5281 tcgaccacga tcgtgcccgg ctgcttttct ccggtcggat tggtgatccg atagcgcgcg
     5341 cgcatcgggt cgaccggagg catcggcgga gcgagcttca tcagttcgag gtcccgaccc
     5401 gacagctgcg gatcggaaaa cgtctcgtac ttgcatcccg cgagaaccag agccaccatg
     5461 gcggcggcag cgatcttaag cgcagtcatt cgtccaaccc attcccgaaa cgagagattc
     5521 acgattagtg aaccataatc cttcatcgct ggttaacgac ttccgttccg tcagaccagt
     5581 gcacgaatgt cacagctgaa caacatctta acaagagtcg ggcgcaaaca ccagatcgtg
     5641 agcgcttgct tcgcctgcgc gcgcggtgca ttcaggggca gcgcatcccg gaggcggccc
     5701 agtggcgacc ctgctgattc acgacacgct gttccagaaa cacgtcatgc cccagggaca
     5761 tccggaacgg ccggcgcggc tcgaagccat cgacaaggcg ctgtccctgc cgctgtttga
     5821 tgccctgaaa cgggaaggcg cccgggcggg cgatccggtg ctggccgagc tggtgcaccc
     5881 cgccgcccat gtccgcgcca tcggc
//
LOCUS       DAOMXN010000163         5865 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_7209_length_5865_cov_9.922892, whole genome shotgun sequence.
ACCESSION   DAOMXN010000163 DAOMXN010000000
VERSION     DAOMXN010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 5865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5865
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7209_length_5865_cov_9.922892"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="PK812_10735"
     CDS             <1..546
                     /locus_tag="PK812_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014285158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRJ70074.1"
                     /translation="WVIGAAAGVAVGFAIGLSSLARSTALPLVSALFPIPKIAMLPLF
                     IIWFGIGEASKYATIAFGVFSPMAIAAFGGVDAVDRTLIRMAQSFGLPPRDIVRKIVL
                     PGAMPALLSGARISASIGIILLTAAEMIGAQHGVGALVLAAGNLMQTDKLVAGVLILS
                     ALGLTVAQIIAIAERRLLKWR"
     gene            complement(556..1032)
                     /locus_tag="PK812_10740"
                     /pseudo
     CDS             complement(556..1032)
                     /locus_tag="PK812_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006332163.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(1058..2815)
                     /locus_tag="PK812_10745"
     CDS             complement(1058..2815)
                     /locus_tag="PK812_10745"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HRJ70075.1"
                     /translation="MKQPLSTRIDGELALALAQLNPTVGDVAGNAAKVRAARKSAAEQ
                     GADIVMLPELFLAGYPPEDLVLKPAFVDVCRAALEDLARETADGGPAVLVGLPWAENG
                     KLFNAFAALDGGRIVGVRFKVDLPNYGVFDEKRVFDVGPMPGPLALRGIRLGLPICED
                     IWGADVVECLTETGAEILLVPNGSPYWRGKTDERFNVAAARVAESGLPLVYLNQLCGQ
                     DELVFDGASFGLNADSSLAFQMGAFAEDIALTRWRRDNGTWRCVLGPKALIPEGEEAD
                     YAACMLGLRDYVNKNGFKGVVLGLSGGIDSALCAAMAVDALGAERVHCIMLPYRYTSA
                     DSFEDAEACAKALGVRYDTLPIAPAVEGFEAILKPLFAGTNRDITEENLQSRTRGTVL
                     MAVSNKFGPMVVTTGNKSEMSVGYATLYGDMNGGFNPIKDLYKMEVYRLSALRNRWKP
                     DGALGPDGEVVPVNILTKAPTAELRENQKDQDSLPPYEVLDAILEMLVEQEMRVAEVV
                     AKGYEIETVRKIERLLYLAEYKRRQSAPGVKVTRKNFGRDRRYPIVNRFRDPGLMAHR
                     PDAAITPQATAASAEGLDF"
     gene            2997..3854
                     /locus_tag="PK812_10750"
     CDS             2997..3854
                     /locus_tag="PK812_10750"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRJ70076.1"
                     /translation="MRLAPALLFTALTATSVQAADYLRGSISAEAPKSASTTIDWSGA
                     YFGVQAAANNAYINQATLANRVGAVSFPNTGVTTTASNLIAFGRTHDIGIGAGGFIGY
                     NTQWDDVIIGMELDYTRGGVRARSTSPTVSRPISDTGSTTRAWDTTLGAQATTVVNDM
                     TTLKLRVGAAFDRFLPYVSGGVAIGRISSRATSTGSTLYYEVQPILDPVTSAIIGYNT
                     INLWPRVNAAGSIKDDGWRWGYNFGAGVDVALTDNFFLRGGWEYSQFGAGQVRTAINA
                     FKGGAAIKF"
     gene            complement(3985..4779)
                     /locus_tag="PK812_10755"
     CDS             complement(3985..4779)
                     /locus_tag="PK812_10755"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009209890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HRJ70077.1"
                     /translation="MAPMPALFVSHGAPNLVLHDSPCHRFLRDIGRSLPRPRAILVVS
                     AHFTTRSPAVTVDAKPATIHDFGGFEPELHAMTYDAPGAPEQARACADLLAAQGISAA
                     LVQGRGFDHGTWVPLKLMVPEADIPVFQLSVQPNAGPAHHLAVGRALQALRGQGVLII
                     GSGAMTHNLHEFFQGGYGLHDPAPDWVRDFGEWMHEQIEAGDTAAMVDYRRASTTGAR
                     NHPTEEHLLPLFTVLGAAGAAPGRRLHSSAQYGVLMMDVYGFGMSA"
     gene            complement(4884..5354)
                     /locus_tag="PK812_10760"
     CDS             complement(4884..5354)
                     /locus_tag="PK812_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020093513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70078.1"
                     /translation="MHLFSSFARKVIALAVIGAGLAVSVAPAEAQWRRKGVRPGVVAA
                     AAVGGLAAGALIASASRPAYGAPVYGYPQYNYAPHAGVTYYSQPTYYAPAYSAPVYSA
                     PVYAPSYATPVVRYHRPRPVYRRYAAYEPVCTITRKKVWLGPNTYTFRRATVCR"
BASE COUNT         1035 a         1955 c         1896 g          979 t
ORIGIN      
        1 tgggtgatcg gcgcggcggc cggcgtggcg gtcggcttcg ccatcgggct gtcctcgctg
       61 gcgcgctcca cggcgctgcc gctggtctcg gccctgttcc cgatcccgaa gatcgcgatg
      121 ctgccgctgt tcatcatctg gttcggcatt ggcgaggcgt cgaaatacgc aaccatcgcc
      181 ttcggggtgt tctcgcccat ggcgatcgcg gcctttggcg gcgtcgacgc ggtcgaccgc
      241 acgctgatcc gcatggcgca gagtttcggc ctgccgcccc gcgacatcgt gcgcaagatc
      301 gtgctgccgg gcgcgatgcc cgcgctcttg tccggcgcgc ggatttccgc ctccatcggc
      361 atcatcctcc tgacggcggc ggagatgatc ggcgcccagc acggcgtcgg cgcgctggtg
      421 ctcgccgccg gcaatctgat gcagacggac aagctggtcg ccggcgtgct gatcctgtca
      481 gccctgggac tgacggtggc gcagatcatc gccatcgccg agcggcggct gctcaagtgg
      541 cggtgaaagg cgtcgctaga gctccagctc ccagaactgg ccgacaaggt ccttgccgaa
      601 ggaatggtgg cgttcctcgc gcaccagcct gaacccccgc tcttcgtaga tgcggcgcgc
      661 cgcgtgcagg atgtcgttgg tccacagtgt cagcaccgcg tagcccttct cgcgcgcgaa
      721 gccgatcacc gcgtccacca gcgcccggcc gagtccgagg ccgcgcgccg aagggtctac
      781 atagagaatg cgcagtttcg cctcgcggtc gctgacgcgc atgacgaaca cggagccgac
      841 gacttcgccg tggcgctccg ccacgaagaa gcgctcgcgc tcggggtcca tcttctgcac
      901 gaagctgccg gcgatctcgg cgaccagcgc ctcgaaggtc aggtcccagc cgtgttcctg
      961 tgcgtagagt tcgccctgcc ggcggatgat ctgtccgaga tcgccgaccc tcggatcgcg
     1021 caacaggaag ggtggggcgg cggcctccgg catcagatca gaagtccagc ccctcggcag
     1081 aggcggcggt ggcctgcggc gtgatggcgg catccggacg gtgcgccatc aggcccggat
     1141 cgcggaagcg gttgacgatc gggtagcgcc ggtcgcggcc gaaattcttg cgcgtcacct
     1201 tgacgccggg cgcggactgg cggcgcttgt actccgcgag atagagcagg cgctcgatct
     1261 ttcgtaccgt ctcgatttcg tagcctttcg ccaccacttc ggcgacgcgc atttcctgtt
     1321 ctacgagcat ttcgagaatg gcgtcgagca cctcgtaggg tggcagcgaa tcctgatcct
     1381 tctggttctc gcgcaactcg gcggtgggcg ccttggtcag gatgttgacc ggcacgacct
     1441 cgccgtccgg tcccagcgcg ccgtccggct tccagcggtt gcgcagcgcg gaaaggcggt
     1501 agacctccat cttgtagagg tccttgatcg ggttgaagcc gccgttcatg tcaccgtaga
     1561 gagtggcgta acccactgac atctctgact tgtttccggt tgtgacaacc atcgggccga
     1621 acttgttcga caccgccatc agcacggtgc cgcgcgtgcg cgattgcagg ttctcctcgg
     1681 tgatgtcgcg gttcgtcccg gcgaacagcg gcttcaggat cgcctcgaac ccttccacgg
     1741 ccggggcgat cggtagggta tcgtagcgca cacccagcgc cttcgcgcag gcttccgcat
     1801 cctcgaagct gtcggcggag gtgtagcggt agggcagcat gatgcagtgc acccgctccg
     1861 cccccagcgc gtccaccgcc atcgcggcgc agagcgccga atcgattccg cccgacagtc
     1921 cgagcaccac gcccttgaag ccgttcttgt tcacgtagtc gcgcaggccc agcatgcagg
     1981 cggcgtaatc ggcctcctcg ccctcgggaa tcagcgcttt tggcccaaga acgcaacgcc
     2041 atgtgccatt gtcccgccgc cagcgcgtca gcgcgatgtc ctcggcgaag gcgcccatct
     2101 ggaaggcgag ggacgaatcg gcgttgagac cgaaggacgc accgtcgaac accagctcat
     2161 cctgcccgca gagctggttg aggtagacca gtggcaggcc actttcggcg acacgcgccg
     2221 ccgcgacatt gaagcgctcg tcggtcttgc cgcgccagta gggcgagccg ttgggcacca
     2281 gcaggatttc cgcgccggtc tctgtgagac actcgacaac atcggcgccc cagatgtctt
     2341 cgcagatcgg caggccgagc cggatgccgc gcagcgcgag cggtcccggc atcggcccca
     2401 cgtcaaagac gcgcttctcg tcgaagacgc cgtagttcgg cagatcgacc ttgaagcgca
     2461 cgcccacaat gcgaccgccg tcgagcgcgg cgaatgcatt gaaaagcttt ccgttttccg
     2521 cccatggcag gcccaccagc acggccgggc caccgtccgc ggtctcgcgg gccagatcct
     2581 ccagcgccgc gcgacagaca tcgacgaagg ccggcttcag caccagatcc tccggcggat
     2641 agcccgccag gaacagttcg ggcagcatga cgatgtcggc gccctgttca gcggcgctct
     2701 tgcgcgcggc gcgcaccttc gccgcgttgc cggccacatc accgaccgtc ggattcagct
     2761 gcgccagggc gagggcgagt tcgccgtcga tgcgcgtgga aagaggttgc ttcatggctc
     2821 cgatctaacg cggcgcaagg ccgcgaacaa ctgtccgccc cgaattgaga cacaccttaa
     2881 ccgggaaaaa cgtcatttcg gcagatgggc ttaaccgggt ggtaaggtta cttttttacc
     2941 gtcgcttatg agttcagcca cctgatttgc gggcggcgtt cataggagac ggcctgatgc
     3001 gcctcgcgcc agcccttttg ttcaccgccc tcaccgccac cagcgtgcag gccgctgact
     3061 atctgcgagg ctcgatttcg gcggaagcgc cgaagtcggc gtcgacgacg attgactgga
     3121 gcggcgccta cttcggcgta caggccgcag ccaacaatgc ctatatcaac caggcgaccc
     3181 tggcgaaccg tgtcggcgcc gtctcgttcc ccaacacggg cgtcacgacg acagcgtcca
     3241 atctgatcgc cttcggcagg acccatgaca tcggcatcgg cgcgggtggc ttcatcggct
     3301 acaatacgca gtgggatgac gtcatcatcg gcatggagct ggactatacc cgcggcggcg
     3361 tgcgggcgcg ctccacgtct cccacggtca gcagaccaat ctcggatacc ggcagcacga
     3421 cgcgtgcctg ggacaccacg cttggcgccc aggccaccac ggtcgtgaac gatatgacga
     3481 cgctgaaact gcgtgtgggc gcggcattcg accgcttcct gccctatgtc tctggcggtg
     3541 tcgcgatcgg gcgcatatcg tcgcgtgcga cgtcgaccgg cagcacactc tactatgaag
     3601 tccagcccat cctggatccc gtgacgtcgg cgatcatcgg ctacaacacg atcaacctgt
     3661 ggccacgcgt gaacgccgcc ggaagcatca aggacgacgg ctggcgctgg ggctacaatt
     3721 tcggcgcggg tgtggacgtc gccctgaccg acaacttctt cctgcgcggc ggctgggaat
     3781 acagccagtt cggcgcgggc caggtccgta ccgccatcaa cgcgttcaag ggcggcgcgg
     3841 ccatcaagtt ctgaccgatc gaagacaacg gacggcacgg gcacgccaac gcctgccccg
     3901 ccgcctgacc atttccattt ccggtctcgc gtatggaaat cggcccgcca catggcgggc
     3961 cttttccgtt gtgccggccg tgccctacgc cgacatcccg aacccgtaca cgtccatcat
     4021 aagaacgccg tactgcgcgc tggagtgcag gcggcggccc ggagcagcgc cggccgcgcc
     4081 gagcaccgtg aacaagggaa gcaggtgctc ttccgtcgga tggttccgcg cgcctgtcgt
     4141 cgaagcgcgg cgataatcca ccatcgcagc ggtgtcgccc gcttcaatct gctcgtgcat
     4201 ccattcgccg aagtcacgga cccaatccgg cgcgggatcg tggagaccgt atccgccctg
     4261 gaagaactcg tgcaggttgt gggtcatcgc gccggacccg atgatcagga cgccctgccc
     4321 gcgcaacgcc tgcaaggcgc ggccgacagc aagatggtgc gccggaccgg cattcggctg
     4381 caccgaaagc tgaaacaccg gaatgtcggc ttccggaacc atcagcttga gcggcaccca
     4441 ggttccatga tcgaatccgc ggccctgcac cagagcggcc gaaatgccct gagcagccag
     4501 caggtccgca caggcccgcg cctgctccgg cgctcccggc gcatcatagg tcatggcatg
     4561 cagttcgggc tcgaagccgc caaagtcatg gatcgtcgca ggttttgcat cgaccgtcac
     4621 cgccggtgac cgcgtggtga aatgcgcgga aacgaccagg atggccctcg gccgcggcaa
     4681 cgaccgcccg atgtcgcgca ggaagcggtg acagggcgaa tcatgcagca ccaagttcgg
     4741 cgcgccatgg gagacgaaca gagcgggcat cggggccatc aggaaactcc tttggcccta
     4801 gctaggtttt cgcggatgca aatacgggcg cgccgtcgca agggtttcct tgcgacggcg
     4861 caagtctcaa ccgcgctgat gcgtcagcgg cagaccgtgg cgcggcggaa cgtgtaggtg
     4921 ttgggtccca gccagacctt cttgcgggtg atggtgcaca ccggctcata ggccgcatag
     4981 cggcgataga ccgggcgcgg gcgatgatag cgcacgaccg gggtcgcgta ggacggagcg
     5041 tagaccggcg cggaatagac cggagccgaa taggccggcg cgtagtaggt cggctgcgag
     5101 tagtacgtca cgccggcgtg gggcgcgtag ttgtactgcg ggtagccgta gaccggggcg
     5161 ccataggcag gccgggacgc cgaagcgatc agggcgccgg cggcaagccc gccaacggcg
     5221 gcagcggcca cgacgccggg gcgcacgccc ttgcggcgcc actgcgcttc agcgggggcg
     5281 acggacaccg caagaccggc tccgatcacc gcaagggcga tcaccttgcg cgcgaatgac
     5341 gaaaacagat gcatggtaca ctcctgcgaa acggccacgc cggggcgcgg actgcctgtt
     5401 atggttgtct ggagactacc cttatccggg cgagtctgtc ccattcctga acaggttctt
     5461 cagaaacatg gtaaatttga gaaggaacag ggaacagacg ggaaaagccc ggcaggggtt
     5521 acctgccggg ccttccgccc caagtgcgcg gcgatcgagc cgccgcgctc cccataagag
     5581 accccggaac cctgggtcgt ccgcgccgga tcagctctcc cccgaccaga cgttacccat
     5641 ttccgaaaga cagggagaaa actagcgcct tcgatttaac gaaccggcaa caaatccggt
     5701 taacggaggg taagccgggt ggagacacca tggcattcct tccgggccgc gaagcggagc
     5761 gcgcaagcac tctttctact gaagatcaaa tgcctggatg gattccggga ccgctccttc
     5821 aagagcgccc cggaatgccc cccatgaggt ggtgccgcct gcccc
//
LOCUS       DAOMXN010000164         5858 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_7227_length_5858_cov_13.615027, whole genome shotgun sequence.
ACCESSION   DAOMXN010000164 DAOMXN010000000
VERSION     DAOMXN010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 5858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5858
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7227_length_5858_cov_13.615027"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(186..1757)
                     /locus_tag="PK812_10765"
     CDS             complement(186..1757)
                     /locus_tag="PK812_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRJ70079.1"
                     /translation="MRSFLYAVALSSALVATASAQTLTIGVRGGPDSIDPHFTATGTH
                     AESLKHIFDTLTWSGDGLEVQPRLAESWKPINDTTWEFKLRKGVKFHDGSDFTAADVK
                     FSIERIPVVAGPNPTTIYVRRVKETKIVDDHTVHIVTDGPAPILPNDFIRLFIVSHKA
                     AAGLTKETANEAFNSGKAAVGTGPYKYVSWQPKGDLVMDRNDAYWGPKEPWARHVRKE
                     IANDAARVAQLKAGQLDLITRVPATDIAALRTDARLAVQTIPTVYVFNVELDMREPAK
                     NLSAKDGSPLPKNPLLDVRVREAIDLAIDRKALAEVAMEGLGVPVNQMVTPSIFGFNK
                     TLGERKHDPAAARKLLADAGYPNGFKIQFSFTNDRLPGDRQVGTSIAQMLAAVGIDAQ
                     ANAQPAAVFFPARTRGEYSMSMSGWGTLTGEAHYTLSSLAHSNDPSVKMGAFNVLGYK
                     NPAMDKLLQDAAIEMDEGKRRKFLEDANALLEKDRQRLPIVAVSSAWAMQKAKVKITP
                     RVDEDTLAMNIKPAN"
     gene            1893..2459
                     /locus_tag="PK812_10770"
     CDS             1893..2459
                     /locus_tag="PK812_10770"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRJ70080.1"
                     /translation="MDRIHSARSRPGRGQPAAAQASAAMLSRERLDAFTHARLWQNPC
                     GFAARFNYLALRYNRPLYGWVESRFGLSRAEFAVIYSLSLLDGLTATEIASSSAFPKN
                     TLSRAVNRLMRLGHLTRRPSADDRRQQVLTLTRSGRLVFREAVPRFVALEEEMLSPLS
                     DGERQVLSELMAKVVLAMFQNHPDDLVE"
     gene            2475..3179
                     /locus_tag="PK812_10775"
     CDS             2475..3179
                     /locus_tag="PK812_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007771279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HRJ70081.1"
                     /translation="MRISDMNWMQVRDHVAKDDRAVLPIGSTEQHAYLSLSVDSILAE
                     RVASESAQPLGVPVFPTLNYGLTPAFVDYPGTVSLRLSTLCALLVDVLDGIVRSGFRR
                     IVIVNGHGGNSPAHGAVLEWLDHHRGVQVKWHNWWNAPRTFAEVQRIDPIASHASWME
                     NFPWTRLAGVAMPDERKPMLDMGSFQQVDPGRKKDLLGDGNYGGLYQRPDEEMLAIWR
                     VAIEETRALIDGGWHR"
     gene            3199..4170
                     /locus_tag="PK812_10780"
     CDS             3199..4170
                     /locus_tag="PK812_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004107451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ70082.1"
                     /translation="MSGFILQRLMQAAVVMLIISALVFTGVYAIGNPIDVLISPDATQ
                     QIREQTIRAYGLDQPLWKQYADFLGRLARGDFGRSFIFNMPVLELILSRLPATLELTL
                     VAVIGATAIGVPLGMYAGYRPDSAVAKLIMAISVLGFSVPTFWIGLVLIYAFAVTLGW
                     LPAGGRGPTVVLFGIEWSVLTLEGWKHLFLPALNLSLFKFALMVRLARAGTREVMLTD
                     TVKFARAVGESEGVILRRHVLRLIAIPIVTVFGLEFGSTLAFAVVTETIFSWPGVGKL
                     IIDSISVLDRPVMVAYLMLVAFLFIIINFLVDIAYAALDPRLRVGRT"
     gene            4167..5048
                     /locus_tag="PK812_10785"
     CDS             4167..5048
                     /locus_tag="PK812_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007752209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ70083.1"
                     /translation="MMDAVTPLGRFWQEFRESHVALAALAVVAAIVLLALAAPLIAPQ
                     NPYDLTQLVLMDARRPPGHVNARGFTYWLGSDAQGRDVLSAILYGLRISMQVGLIAGA
                     IAFSLGATLGAFAAYWGGRVEMVIMRFVDLQLSFPAILLALVLAAVLGQGKWQLIAAL
                     VAAQYAYFARTTHGAASSERAKDYVAAALSTPLPARTVVLRHILPNCLPPLIVVATVQ
                     VANSIALEATLSFLGLGLPVTEPSLGMLIANGFQYLLSGRYWISIYPGVALIVLIVAI
                     NLVGDQVRDQLNPRLKR"
     gene            5045..>5858
                     /locus_tag="PK812_10790"
     CDS             5045..>5858
                     /locus_tag="PK812_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ70084.1"
                     /translation="MSTLLEVRDLSTHFETKAGVVKAVDGVSFSLSAGEIIGIVGESG
                     SGKTVTGFSILGLVDPPGRIVAGSVRLDGQDLVGMAPGKLRALRGRRMAMVFQDPSAT
                     LNPVLTIGAQMRLAIAAHEKIGEAAMRARCIAVLGRVGIPDAARRLEAHPHQFSGGMR
                     QRVAIAIALLNRPDIIICDEPTTALDVSIQAQILNEMKTLARDLGTALVWISHDLATV
                     SSMASRILVMYAGRIIEEGPTARVLRGPRHPYTRGLLDSLPAVTTPGRDLAQI"
BASE COUNT          868 a         1856 c         1977 g         1157 t
ORIGIN      
        1 gcacgctcca ccccgcgcgc agggaccgcg ccagatgctg gcgcgcctcg gcccattccg
       61 cctcgctcat cagcgtgcgg tccagcgtgc cgcagccgcc gtggatgccg atggcgatgt
      121 cagtcatcac gtcccccggg aaaacctggc cccggacaag taaccggggc caagcgggat
      181 gccggtcagt tcgccggctt gatgttcatc gccagcgtgt cctcgtcgac gcgtggggtg
      241 attttcacct tggccttctg catggcccag gcagaactca cggcgacgat cggcaggcgc
      301 tggcggtcct tctcgagcag cgcgttcgca tcttcgagga acttgcgtcg cttgccctca
      361 tccatttcta tcgccgcgtc ctgaagcagc ttgtccatgg cggggttctt gtagcccagc
      421 acgttgaacg cgcccatctt cacagacgga tcgttcgaat gggccaacga cgagagcgtg
      481 tagtgcgcct cgcctgtcag cgtgccccag ccggacatcg acatggaata ttcgccgcgc
      541 gtgcgggccg ggaagaacac cgctgccggc tgtgcgttgg cctgggcatc gatgccgaca
      601 gccgcgagca tctgggcgat ggaggttccg acctgccgat cgccaggcag acggtcattg
      661 gtgaaggaga actggatctt gaagccgttg ggatagccgg cgtcggccag cagcttcctg
      721 gcggcggcgg ggtcgtgctt gcgctcaccc agtgtcttgt tgaagccgaa gatcgaaggt
      781 gtcaccatct ggttgaccgg caccccgagc ccttccatcg ccacttcggc aagagccttg
      841 cggtcgatgg ccaggtcgat ggcttcgcgc acgcgcacgt caagcagcgg attcttcggc
      901 agcggcgagc cgtccttggc gctgaggttc ttcgccggtt cgcgcatgtc gagctcgacg
      961 ttgaagacat agacggtggg gatcgtctgg accgcaagcc tggcatcggt ccgcagcgcg
     1021 gcgatgtcgg tcgccggcac gcgcgtgatc aggtcgagtt gccccgcctt gagctgcgcg
     1081 acgcgcgcgg catcgttggc gatctccttg cggacgtggc gcgcccaggg ctccttcgga
     1141 ccccagtagg cgtcgttgcg gtccatgacg aggtcgccct tgggctgcca ggagacgtac
     1201 ttgtaaggcc cggtgccaac tgccgccttg ccggagttga aggcctcgtt cgccgtttcc
     1261 ttcgtcaggc cggcggctgc cttgtgcgag acgatgaaca ggcggatgaa atcgttcgga
     1321 aggatcggcg ctggcccatc ggtgacgata tggacggtgt ggtcgtccac gatcttcgtc
     1381 tccttgacgc ggcgcacgta gatcgtcgtc gggttcgggc ctgccacgac cggaatacgc
     1441 tcgatggaga acttgacgtc cgcagcggtg aagtccgacc catcgtggaa cttgacgccc
     1501 ttgcgcagct tgaattccca ggtggtgtcg ttgatcggct tccagctttc ggcgaggcgc
     1561 ggctgcactt cgagcccgtc gcccgaccag gtgagcgtgt cgaagatgtg cttgagagat
     1621 tccgcgtggg ttccggtggc cgtgaagtgc ggatcgatgg aatccggccc cccgcgcacg
     1681 ccgatggtca atgtctgggc ggaagcggtt gcgacgagcg ctgacgacaa ggcgactgcg
     1741 taaagaaacg aacgcattct gacccctccc aagggaatgc ccggaccgcc atggcccggc
     1801 tcgtgaacgg tcattgaagg acggcccgcc cggacagtca attccttgtg gaactaattt
     1861 tgcgcgctat gctccggcaa ggggggcgag cgatggaccg cattcattcc gcaaggtcgc
     1921 gtccgggtcg gggccagccg gccgcggcgc aggccagcgc cgcgatgctg tcgcgcgagc
     1981 gcctcgacgc gttcacgcat gcgcggttgt ggcaaaaccc ttgtggtttc gccgcccggt
     2041 tcaactatct ggcgctgcgc tacaaccgac cgctctacgg ctgggtggag agccgcttcg
     2101 gcctttcgcg ggccgagttt gcggtaatct actcgctctc gctgctggat gggctgacgg
     2161 cgaccgagat cgcctcctcc tccgcatttc cgaaaaacac gttgagtcgc gccgtgaacc
     2221 ggctgatgcg gctcggccac ctgacgcgcc ggccgagcgc cgacgaccgg cgtcagcagg
     2281 tgctgaccct gacgcggtcg gggcggctgg tgtttcgcga ggcagtgccg cgtttcgtgg
     2341 cgctggaaga agagatgctg tcgccgctgt ccgatggcga gcggcaggtg ctgtccgaac
     2401 tcatggcgaa ggtggtgttg gcgatgttcc agaaccaccc cgacgatctg gtggaatgaa
     2461 ggctggggga agtgatgcgc atatctgaca tgaactggat gcaggtgcgt gaccatgtcg
     2521 cgaaggatga tcgcgccgtg ctgccgatcg gttccaccga gcagcacgcc tatctcagcc
     2581 tcagcgtcga ctcgatcctc gccgagcgcg tggcgagcga gtcggcgcag ccgctcggcg
     2641 tgccggtgtt tccgacactg aactacgggt tgacgccggc gttcgtcgac tatcccggca
     2701 cggtgagcct gcgcctctcc accctctgcg ccctgttggt cgacgtgctg gatggcatcg
     2761 tccgttctgg ctttcgcagg atcgtgatcg tcaatggtca cggcggcaac tcgccggcgc
     2821 acggcgccgt gctggaatgg ctcgatcacc atcgcggcgt gcaggtgaag tggcacaact
     2881 ggtggaacgc gccccggacc ttcgccgagg tgcagcgtat cgacccgatc gcgtcgcatg
     2941 cctcgtggat ggagaatttc ccctggaccc ggctggcggg cgtcgccatg ccggacgaac
     3001 gcaagccgat gctggacatg ggctccttcc agcaggtcga tcccgggcgg aagaaagatc
     3061 ttctgggcga tggcaactat ggcggtctct atcagcgtcc ggacgaggag atgctggcga
     3121 tctggcgggt ggcgatcgag gaaacccgcg cgctgatcga tgggggatgg catcggtgat
     3181 cggacaaggg gccttctgat gtccgggttc atcctccagc ggctgatgca ggctgcggtg
     3241 gtgatgctga tcatctcggc cctggtgttc acgggcgtct atgcaatcgg caatccgatc
     3301 gacgtgctga tctcgcccga tgcgacgcag cagatccgcg agcagaccat tcgcgcctat
     3361 ggcctcgatc agccgctgtg gaagcagtac gcggattttc tcggccggct ggcgcgcggc
     3421 gattttggac gctccttcat cttcaacatg ccggtgcttg agctgatcct gtcccgcctg
     3481 cctgccacac tggaactgac gctggttgcg gtgattggcg cgacggccat cggcgtgccc
     3541 ctgggcatgt atgccggcta tcggccggac agtgccgtcg ccaagttgat catggcgatt
     3601 tccgttctag gcttctcggt gccgacgttc tggatcgggc tggtgctgat ctatgccttc
     3661 gcggtcacgc tgggctggct gccggcaggc gggcgcggtc cgacagtcgt gctgttcggc
     3721 atcgaatgga gtgtcctgac gctggaggga tggaagcacc tgttcctgcc ggcgctcaac
     3781 ctgtcgctgt tcaagttcgc gctgatggtg cgcctcgctc gcgccggcac gcgggaagtg
     3841 atgctgacgg atacggtcaa gttcgcccgt gccgtgggcg agagcgaggg cgtgatcctg
     3901 cgccgccatg tcctgcgtct catcgctatc ccgatcgtca cggtgttcgg cctcgagttc
     3961 ggatcgacgc tggccttcgc cgtggtgacc gaaacgatct tctcctggcc gggcgtcggc
     4021 aagctgatca tcgattccat ttcggtgctc gaccggccgg tgatggttgc ctacctgatg
     4081 ttggtcgctt tcctgttcat catcatcaac ttccttgtgg acatcgccta tgcggcgctc
     4141 gatccgcgcc tgagggtcgg gaggacatga tggatgccgt gacgccgctt ggccgcttct
     4201 ggcaggagtt tcgcgagagc catgtggcgc tggcggcgct cgccgtggtc gcggcgatcg
     4261 tcctgctggc gcttgccgcc ccgctgatcg cgccccagaa cccctatgac ctgacgcagt
     4321 tggtgctgat ggatgcccgc cgcccgccgg gtcatgtgaa tgcccgcggg ttcacctact
     4381 ggctggggtc cgacgcgcag gggcgcgacg tgctgtcggc cattctttac gggctgcgca
     4441 tctcgatgca ggtcggcctg atcgcgggtg ccatcgcctt cagcctcggg gcgacgctcg
     4501 gcgccttcgc cgcctattgg ggcggccgcg tcgagatggt catcatgcgg ttcgttgacc
     4561 tgcagctgtc gtttccggcc atcctgctgg cgttggttct tgcggccgtg ctggggcagg
     4621 gcaagtggca gctgatcgcc gcgctggtgg cggcgcagta tgcctatttc gcgcgcacga
     4681 cccatggcgc ggcgtcgtcg gaacgcgcca aggattacgt cgctgccgcc ctgtcgacgc
     4741 cgctgccggc ccgcacggtc gtgctgcggc atatcctgcc gaactgcctg ccgccgctga
     4801 tcgtggtggc gacggtgcag gtcgccaatt ccatcgcgct ggaagcgacg ctgtcgttcc
     4861 tcggtctcgg cctgccggtc accgagccct ccctcggcat gctgatcgcc aacgggtttc
     4921 agtacctgct gtccgggcgc tactggatct cgatctaccc cggggtcgcg ctgatcgttc
     4981 tgatcgtggc gatcaacctc gtcggcgatc aggtgcgcga ccagttgaac ccgaggctga
     5041 agcgatgagc acgctgctgg aggttcgcga cctgtcgacg catttcgaaa ccaaggccgg
     5101 cgtggtgaag gcggtcgacg gggtttcctt ctcgctctcg gccggagaga tcatcggcat
     5161 cgtcggggag tcgggctcgg gcaagaccgt gacgggattc tcgatcctcg gtcttgtcga
     5221 tccgcctggg cggatcgtcg ccgggtcggt ccgactcgac ggtcaggacc tggtcggcat
     5281 ggcgccggga aagttgcgcg cattgcgtgg caggcgcatg gcgatggtgt ttcaggaccc
     5341 gtccgccacg ctcaatccgg tgcttaccat cggcgcgcag atgcgccttg ccatcgccgc
     5401 gcatgagaag atcggcgaag cggcgatgcg ggcgcgctgc atcgccgtgc tcgggcgggt
     5461 cggcattccg gacgcggccc gccggcttga ggcgcatccg caccagtttt ccggcggcat
     5521 gcgccagcgc gtggccatcg ccatcgccct gctgaaccgc ccggacatca tcatctgcga
     5581 cgaaccaacc accgccctgg acgtttcgat ccaggcgcag atcctgaacg agatgaagac
     5641 gctggcgcgc gacctcggca cggctctggt atggatcagc cacgatcttg caaccgtctc
     5701 ctccatggcg agccgcatcc tggtgatgta cgccgggcgc atcatcgagg aagggcctac
     5761 cgcgcgcgtg ctgcgcgggc cgcgtcatcc ctatacgcgc ggacttctcg attccctgcc
     5821 tgccgtgacc acgcccggcc gcgatctcgc ccagatcc
//
LOCUS       DAOMXN010000165         5840 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_7262_length_5840_cov_9.757995, whole genome shotgun sequence.
ACCESSION   DAOMXN010000165 DAOMXN010000000
VERSION     DAOMXN010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 5840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5840
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7262_length_5840_cov_9.757995"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="PK812_10795"
     CDS             <1..830
                     /locus_tag="PK812_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009489351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRJ70085.1"
                     /translation="GHLEEAKRMPCRAVTALPGGRIGIAPHSLRAVDPASLRVVLDAC
                     PSGPVHIHAAEQVKEVEDSVAWSGKRPVEWLLDATEIGPRWCFIHATHMTEAETRALA
                     ATGAVAGLCPITEASLGDGIFNAVDFTRSGGRFGVGTDSNIEITASGELKQLEYSQRL
                     RHRARNVLPQRQGESTGLTLYRAAVAGAAQALDRGKGGIAVDESADFVVLDRNHPDLA
                     FVAGDRWLDAHVFSAGRAVVDQVYVRGERMVEAGRHRARGAIAARYASTMRRIAEMA"
     gene            860..1591
                     /gene="hutC"
                     /locus_tag="PK812_10800"
     CDS             860..1591
                     /gene="hutC"
                     /locus_tag="PK812_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine utilization repressor"
                     /protein_id="HRJ70086.1"
                     /translation="MDHADPIPRYLEIQRDLQDRIASGEWPPGHRVPPEHELQARYGC
                     SRMTVNKALSTLAGVGLIERRRRSGSFVAAPKSQQTILKIHDIKAEIAASGRPYRFSI
                     LSRTRRRMSADDATRLGIAQDGEVEALDVIHFAGETPFVMEERLINLKVAPGARGESF
                     VDSPPGTWLLNLVPWTDAEHIIRAEPVSGPMALRLAMSSRGACLVIERRTWQAGEAIT
                     WVKLTYPGERHRLTSRFSPGDRIGG"
     gene            complement(1595..2377)
                     /locus_tag="PK812_10805"
     CDS             complement(1595..2377)
                     /locus_tag="PK812_10805"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ70087.1"
                     /translation="MSKLVVENVSRRFEGVRGGKPTLALQPTDVVIGDNDFVTILGPS
                     GCGKSTLLRMVAGLDRPTTGTIRLDGREVVGPGPDRGMVFQSYTLFPWLTVAENIAFG
                     LREKGMPEARQKEIVSAYVDRVGLKGFENHWPKQLSGGMQQRTAIARALANDPAILLL
                     DEPFGALDNQTRALMQELLLGIWEREKKTVLFVTHDIEEAIFIASRCLVMSARPGRIK
                     ADLRIDLPYPRHYTIKTSPEFSALKARLTEEIRAEAVLAAEH"
     gene            complement(2374..3147)
                     /locus_tag="PK812_10810"
     CDS             complement(2374..3147)
                     /locus_tag="PK812_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ70088.1"
                     /translation="MRPLEPVSPAAKAMLGISFFALFFAGWAVATFGGFVPKIFLADP
                     LAMLRDGWTLLAKHGFLYDITITIWRVVGGFILAAVVAVPLGVLMGAYKPVEAFFEPF
                     VSFARYLPASAFIPLLILWAGIGETQKLLVIFIGSVFQIVLMVAVAVGNTRRDLVEAA
                     YTLGSGDAGVVKRVLIPMNAPEVAEILRLVLGWAWTYVIVAELIGSSAGIGHMIIDSQ
                     ALMATGQIIFGIIVIGVIGLISDYLFKLMNRRLFPWRLA"
     gene            complement(3379..4320)
                     /locus_tag="PK812_10815"
     CDS             complement(3379..4320)
                     /locus_tag="PK812_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRJ70089.1"
                     /translation="MNKIVLAAALAVTTAAPALAQETKVSIGISGWTGFAPLTLAKEA
                     GIFKKNGLDVTIKKIPQASRHLAILSGDIQCAATTVETWVVWNANGVPTKQIFQLDKS
                     YGADGMVVRSSIGSVKDLKGKTVAASAPGTAPYFTLAWFLKKNGLTIKDVNVVNMEPG
                     PAAQAFIAGQNDAAMTYEPFLSAVRAAPQSGKIIATTLDYPMIMDTFGCTPKFLAENE
                     KAAKALADSYFEAVKMIASDPKKSYEIMGADVKQSGEDFAKSASYLRWQDKEANKKFF
                     AGEHAAFSNEAADLLLELGIIKQKPDMSQLADTRFIQ"
     gene            4652..5458
                     /locus_tag="PK812_10820"
     CDS             4652..5458
                     /locus_tag="PK812_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70090.1"
                     /translation="MLPRTGSPRWDAVAAAVDGPAAEGVRSHEAAADALLLRPAACHE
                     FYAAQEADGIAGLGAILALRKTNHARSVLLWVRPATLSGEVGQVSPAGLAEYGQDPAA
                     MVLVRVKDVRQALKAGLEGARCTALGSVLIELRGDAKSYDLTASRRLALAAKASGASV
                     LLLRFSARPCPSAAETRWLVQAAPSRALAANAPGHPAFDLTLLRARSGRQGLCYRVEW
                     NRNVCSFLASPRAAGDAGRSGPDTSLPGAVVPVSFSRPDRALGERRRDAG"
     gene            5391..>5840
                     /locus_tag="PK812_10825"
     CDS             5391..>5840
                     /locus_tag="PK812_10825"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HRJ70091.1"
                     /translation="MWFPYLSADRTERWGSADATQAEVPLVLVEKAQGALRIAAMNPI
                     AAQAALRPGMALAEARALLTRLRVEEMDRAADHALLLELATACERFTPLVALRDGDGL
                     LLDITGCTHLFGGEGALMGQARRVLRRLGVSSRAALAGTPDAAWAFAR"
BASE COUNT          978 a         1893 c         1939 g         1030 t
ORIGIN      
        1 ccggtcatct cgaggaggcc aagcgaatgc cctgccgcgc cgtcaccgcc ctgccgggcg
       61 ggcgcatcgg catcgcgccg cactcgctgc gcgcggtgga ccccgccagc ctgcgggtgg
      121 ttctggatgc ctgcccttcc ggccccgtgc atatccatgc ggcagagcag gtgaaggagg
      181 tcgaggacag tgtcgcatgg tccggcaagc ggccggtcga atggctgctc gacgcaaccg
      241 agattggccc gcgctggtgc ttcatccacg ccacccacat gacggaagcc gagacgcgcg
      301 cgcttgctgc gaccggagcg gtggccggcc tgtgcccgat caccgaggcg agcctcggcg
      361 atggcatctt caacgccgtg gactttaccc gctccggcgg acgtttcggc gttggcacgg
      421 attccaacat cgagatcacc gcatcgggcg aattgaagca gctcgagtac agccagcggc
      481 tcagacaccg cgcgcgcaac gtgctgccgc agcgacaggg cgaatcgacg ggcctgacgc
      541 tgtaccgcgc cgccgtcgcc ggcgcggcgc aggcgctgga tcggggcaag ggcggaattg
      601 cggtggacga atcggcagat ttcgtcgtgc tcgaccgcaa ccatcccgac cttgccttcg
      661 tcgccggcga ccggtggctg gacgcccacg tcttctcggc cgggcgggcc gtggtcgacc
      721 aggtctatgt gcgcggtgaa cggatggtcg aggccggccg tcaccgcgcc cgtggcgcga
      781 tcgcggcgcg ctacgcctcg acgatgcgac gcattgccga aatggcatga tccgggctag
      841 gcggaggcag gaggccggtt tggaccacgc cgatcccatt ccccggtacc tcgagatcca
      901 gagggacctg caggaccgca tcgccagcgg cgagtggccg cccggtcacc gtgtgccgcc
      961 cgaacatgaa ttgcaggcgc gctacggctg ctcgcgcatg accgtcaaca aggcgctctc
     1021 gacgctcgcc ggcgtgggcc tgatcgagcg tcgccgccgt tccgggtcct tcgtcgccgc
     1081 accgaagtcg cagcagacga tcctgaagat tcatgacatc aaggccgaga tcgccgccag
     1141 cggccggccg tatcgcttct cgatcctgag ccgcacgcgc cggcgcatga gtgccgacga
     1201 tgcaacgcgt ctcggcatcg cgcaggatgg cgaagtcgag gcgctggacg tcatccactt
     1261 cgcgggcgag acgcctttcg tgatggagga gcggctgatc aatctgaagg ttgctccggg
     1321 cgcgaggggt gaatcgtttg tagattcccc gcccggcacc tggttattga atctcgtgcc
     1381 ctggacggat gccgagcaca tcatccgcgc cgaaccggtt tccgggccga tggcgctgcg
     1441 tcttgccatg tcgagccgcg gcgcctgtct ggtgattgaa cggcggacct ggcaggcagg
     1501 cgaagccatc acctgggtca agctcaccta ccccggtgaa cgccaccggc tcaccagccg
     1561 cttcagtccc ggggaccgga tcggcggata ggtctcagtg ctcggccgcc agcacggcct
     1621 cggcccggat ttcctcggtc aggcgcgctt tcagcgccga gaactcaggc gaggtcttga
     1681 tcgtgtagtg gcgcggataa ggtagatcta tgcgcagatc cgccttgatc cgccccgggc
     1741 gcgcgctcat caccaggcag cgcgaggcaa tgaagatcgc ctcctcgatg tcgtgggtga
     1801 cgaacagcac cgtctttttc tcgcgctccc agatgccaag cagcagctcc tgcatcagcg
     1861 cacgggtctg gttgtcgagc gcgccgaaag gctcgtcgag cagcaggatc gccggatcat
     1921 tggcgagcgc ccgggcaatg gccgtgcgct gctgcatgcc gccggacaat tgcttgggcc
     1981 agtggttctc gaaccccttg aggccgaccc tatcgacata agccgagacg atctccttct
     2041 gccgtgcctc gggcatcccc ttttcgcgca ggccgaaggc gatgttctcc gccacggtca
     2101 gccagggaaa gagcgtgtag ctctggaaca ccatcccgcg atccggcccc ggaccgacga
     2161 cctcccgccc atcgagccgg atcgtgcctg ttgtcggacg gtcgaggccg gcgaccatgc
     2221 gcagcagcgt cgactttcca caccccgagg ggccgagaat ggtgacgaag tcgttgtcgc
     2281 cgatgacgac atcggttggt tgcagggcca gggtcggctt gccgccgcgg acgccttcga
     2341 accggcgtga gacattctcg acgacgagct tgctcatgcc agcctccagg ggaacagcct
     2401 gcggttcatg agcttgaaca ggtagtccga gatcaggccg atgacgccga tgacgatgat
     2461 gccgaagatg atctgtccgg tcgccatcag ggcctgcgaa tcgatgatca tgtgaccgat
     2521 ccctgcggag gagccgatga gctcggcgac aatcacgtag gtccaggccc agcccagcac
     2581 gaggcgcagg atttcggcca cttccggcgc attcatcggg atcaggaccc gtttcaccac
     2641 accggcatcg ccgctgccga gcgtgtaggc tgcctcgacc agatcgcgtc gcgtgttgcc
     2701 gaccgcaacc gccaccatca gcacgatctg gaataccgag ccgatgaaga tcacgagcag
     2761 cttctgggtt tcgccgatgc cggcccacag gatgagcagg gggatgaagg ccgatgccgg
     2821 caggtagcgc gcaaaggaga cgaacggctc gaagaacgcc tcgaccggtt tgtacgcgcc
     2881 catcagcacg cccagcggca cggcgacgac cgctgccagg atgaaccccc cgacgacgcg
     2941 ccagatcgtg atggtgatgt catagaggaa gccgtgcttg gccagcagcg tccagccgtc
     3001 gcgaagcatc gccagcggat cggccaggaa gatctttggc acgaagccgc cgaacgtcgc
     3061 caccgcccag ccggcaaaga aaagcgcgaa gaaactgatt cccagcatgg ctttggccgc
     3121 cggagagacc ggttcgaggg ggcgcaaggg atccgctcct gtcctgtgga gaaacgcgat
     3181 gcgtcacgac ttgacccgag gttgcggggc aatccggcct gatgcaaccg gattgccccg
     3241 cgcacctgtc gatgcgaggc gcctgatgcc aaggctcatg acacttgagc cttggttcct
     3301 gttttgaatt ttcatctccc atcggaaagc aatgaaaatt caaaagtcat catcggtcac
     3361 ttcacgtgac ggacggcatt actggatgaa gcgcgtatcg gcgagctggc tcatgtccgg
     3421 cttctgcttg atgatgccga gttcgagcag caagtcggcc gcctcgttgg agaaggccgc
     3481 gtgctcgccg gcgaagaact tcttgttcgc ctccttgtcc tgccagcgca ggtaggaagc
     3541 ggacttggcg aaatcctcgc ctgactgctt cacgtccgcg cccatgatct cgtacgactt
     3601 cttgggatcg gaggcgatca tcttgaccgc ctcgaagtag ctgtcggcga gggctttggc
     3661 cgccttctcg ttttcggcaa ggaacttcgg cgtgcagcca aacgtgtcca tgatcatcgg
     3721 atagtcgagc gtggtggcga tgatcttgcc ggactggggt gcggcgcgta cggcagagag
     3781 aaatggctca taggtcatgg ccgcatcgtt ctgaccggcg atgaaggcct gcgcggcagg
     3841 gcctggctcc atgttgacga cattcacgtc cttgatggtc aggccgttct tcttcaggaa
     3901 ccaggcgagg gtgaaatagg gcgcggtgcc gggcgccgag gcggcgacgg tcttgccctt
     3961 caggtccttg accgaaccga tcgaagagcg cacgaccatg ccgtcggcgc catagctctt
     4021 gtcgagctgg aagatctgct tggtcggcac gccgttggcg ttccatacca cccaggtctc
     4081 caccgtcgtc gcggcgcact ggatgtcgcc ggagaggatg gcaaggtgac gggaggcctg
     4141 cggaatcttc ttgatggtga cgtcgagccc gttcttcttg aagatgccgg cctccttggc
     4201 cagcgtcagc ggcgcaaaac cggtccatcc cgaaataccg atcgaaacct tggtctcctg
     4261 cgccaacgcc ggagcggcgg tggtgacggc cagtgccgcc gcgagtacga tcttgttcat
     4321 ccctgtctcc cgttgttcgg cctacgagcc tggataggaa gaaagcactg gcgcgtttaa
     4381 ttgtctagac aattgtaggt gccgagatgc gccgacctgc tcctcccgcg ggctttctgg
     4441 caaagcgacg cccgccacgc aaccatgggc tggcccccgg ccaacaggaa atctcctgcg
     4501 gcatcgtcga tttattgcga ctatccagcg acttacggga ccttgacggc tgacggatgg
     4561 tttctgctgc gctcttgcgt ccctcgtgct gtcgagcctg ccttgactct cggcgggcct
     4621 tgcgggattt tagaacaaaa agggaacata tatgctgcct cgtaccggat caccccgctg
     4681 ggatgccgtt gccgccgccg ttgacgggcc ggcggcggag ggcgtgcgtt cccatgaggc
     4741 cgcagccgac gctctgctgc tgcggcctgc cgcctgtcac gaattttatg ccgcccagga
     4801 agcggacggg atcgccgggc tcggcgcgat cctggccctg cgcaagacaa accatgcccg
     4861 ttccgtcctg ctctgggtgc gcccggccac cctctcgggc gaggtcggac aggtttcacc
     4921 cgccggtctg gctgaatacg ggcaggatcc cgccgccatg gtgctggtgc gggtcaagga
     4981 tgtccgccag gcgctgaagg ccggacttga aggggcgcgc tgcacggcgc tcggcagcgt
     5041 gctgatcgag ttgcggggag acgcgaagtc ctacgacctg acagccagcc gccgtctggc
     5101 actggcggcc aaggcttcgg gcgccagcgt tttgctgctg cggttttccg cccggccgtg
     5161 cccaagcgcg gcggagaccc gctggctggt gcaggcggct ccgtcgcgcg cgctggcggc
     5221 caatgcgccc ggtcatccgg cgttcgacct gacactcctg cgtgcccgca gcggccgcca
     5281 ggggctttgc taccgagtgg agtggaatcg caatgtctgc agcttccttg ccagtccccg
     5341 ggccgcaggc gacgctggca gatccgggcc cgacacgtcg ctacctggcg ctgtggttcc
     5401 cgtatctttc agccgaccgg acagagcgtt gggggagcgc cgacgcgacg caggctgagg
     5461 tccccctcgt cctggtggag aaggcgcagg gcgccctgcg gatcgccgcc atgaatccta
     5521 tcgccgcgca ggccgccctg aggccgggca tggcgctggc cgaagcgcgg gccttgctga
     5581 cgcggctgcg ggtcgaagag atggaccggg ctgccgatca cgctcttttg ctggagcttg
     5641 caaccgcctg cgaacggttc acgccgctgg tcgccctgcg ggatggcgac ggcctgctgc
     5701 tcgacattac cggctgcact catctgttcg gcggcgaagg agcgctcatg ggtcaggccc
     5761 gtcgggttct gcggcgcctc ggcgtgtcga gccgggccgc gcttgccgga acgccggatg
     5821 ccgcctgggc cttcgcgcgg
//
LOCUS       DAOMXN010000166         5835 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_7274_length_5835_cov_10.676644, whole genome shotgun sequence.
ACCESSION   DAOMXN010000166 DAOMXN010000000
VERSION     DAOMXN010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 5835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5835
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7274_length_5835_cov_10.676644"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1262
                     /locus_tag="PK812_10830"
     CDS             <1..1262
                     /locus_tag="PK812_10830"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012778796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HRJ70092.1"
                     /translation="AKTGAERAMILGRLAELIRANTEELAQILTAEQGKPLAESRGEV
                     GMSAAYVQWFAEEARRIYGDTIPSPWGDRRIVVTKEPIGVVAAITPWNFPSSMIARKL
                     GAALAAGCTIVFKPAELTPYSGLAWGVLCEKAGVPAGVANFITGTPAEIGAEFTANPV
                     VRKITFTGSTAVGKLLMKQSADTMKRISLELGGHAPFIVFDDADVDLAVEQAIASKYR
                     NTGQTCVCTNRFIVQDGVYAAFADRFAAAVRTLKVGAGTEQGVAQGPLISEAALAKVE
                     SHVEDAARKGGKLLSGGKRHALGRTFYEPTVIAEATRDMLIASEETFGPVAPLFRFKD
                     EAEAVALANDTPYGLAGYFFTRDLARAFRVAEALQYGQVGINAGVITSEVAPFGGVKE
                     SGMGREGSKYGLDEYLSVKYLCFGGLR"
     gene            1467..3191
                     /gene="araD"
                     /locus_tag="PK812_10835"
     CDS             1467..3191
                     /gene="araD"
                     /locus_tag="PK812_10835"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinonate dehydratase"
                     /protein_id="HRJ70093.1"
                     /translation="MTDRDPKKLRSHRWFGTDDIRAFGHRSRMVQAGLAHEEFVGRPC
                     IGIINTWSDINTCHTHLRERAQEVRRGVLQAGGFPVELPALSLSEQYVKPTTMLYRNL
                     LAMETEELIRCHPLDGVVLLGGCDKTTPALLMGAASANVPAIYVPAGAMLRGNYRGQI
                     LGSGSDVWKYWHEKEAGNISKAQWDSMELGIARSFGTCMTAGTAATMMSIADALGMTL
                     PGASSIPAADAAHPRMAAACGRRIVDMVWEGLTPRKVLTRAAFENALVTHMALAGSTN
                     AIIHLVAMAGRAGVKVDLADFETYSRRVPVIANLRPSGAYLMEDFFYAGGLPGFLNVL
                     KPFLNLDAMTVTGKTLGDNIDGAEVFNQDVIRPLDKPIAASGGTAVLYGNLAPKGCVM
                     KPPAAEARLLKHTGPAIVFDTYEAMKKAVDDEGLDVTKDHVMVFRNAGPVGGPGMPEW
                     GMLPLPKKLLKQGVRDMIRISDARMSGTSYGACILHVSPEAYVGGPLAAVRNGDLISI
                     DVEARTIHLHVDDAEIARRVAAYERPDRDFPRGYGKMYAAHIKQAHDGCDFDFLEGTQ
                     PIPEPAIF"
     gene            3215..3511
                     /locus_tag="PK812_10840"
     CDS             3215..3511
                     /locus_tag="PK812_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70094.1"
                     /translation="MKSRRVCVEDLGRPLLAALPLARAGKPRAAVLTAEHLRQQRSPQ
                     QRRHVAMRLAGVADTGSRYPIITDIEHATILAAINAGALTLRDENGRMLARSTE"
     gene            3508..4317
                     /locus_tag="PK812_10845"
     CDS             3508..4317
                     /locus_tag="PK812_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRJ70095.1"
                     /translation="MTRKPQRLLITGAAGMLGKQLRRRLAGEYALLRLSDVASMEPAG
                     AGEETVVCDLADREGVGRLCEGIDAIVHLGGRATEADWDTVHSANILGAIHLYEGARK
                     AKVERVLFASSNHAVGLVRRTPKTDHTASARPDSRYGLSKAFGEDIAALYAYKHGVRA
                     YCMRIGSCFPEPTDERMLSSWLSYDDFERLVRTGLAADYTYEIVYGISRNTRAWWDNS
                     NAYRLGYNPQDNAEAFAPRLTGVGVGNPLHDPFIGGGYVSPDFEADPWKIP"
     gene            4422..5156
                     /locus_tag="PK812_10850"
     CDS             4422..5156
                     /locus_tag="PK812_10850"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HRJ70096.1"
                     /translation="MNETAKSLGPVSGTQSRTGDLVRMLSEDIRSGRIRPGEKLPTEQ
                     EIVRMTGVSRTVVREAVAALRAEGLVVTRQGVGAFVTAAPGQGMFKISAEEAGSLERV
                     LQILELRIGMEVEAAGLAAERADREAVARIAGHQAEFNAGAQNGDIVIGADYGFHRAV
                     SEATRNPYFPRILNSLGQILIPRGNVPSSLPTKEERRSYLMRIAGEHEAILAAIRKRD
                     VEGARSAMRHHLVASRERYQLLAEKS"
     gene            complement(5193..>5835)
                     /locus_tag="PK812_10855"
     CDS             complement(5193..>5835)
                     /locus_tag="PK812_10855"
                     /inference="COORDINATES: protein motif:HMM:NF014640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HRJ70097.1"
                     /translation="LPGPEALLAGPRQRLDVTSLKLSGAVRSGVERRNGLIGVLARRL
                     AAQAPQARLARFAERISGLGALLERSLARRTELRRQRLDAVGKSLAVAFAARLAGERR
                     DMLRRRERLVALAERLPRALGMRQADRAARLHRLDSLLGSLGYRQVLARGFALVRDGD
                     GKAVRHAAAVSEGMALRIEFADGEVATTAGEGARPKSRAKQPAAPSGGQGSLF"
BASE COUNT         1034 a         1948 c         1901 g          952 t
ORIGIN      
        1 gggcaaagac cggcgccgaa cgcgccatga ttctgggcag gttggccgag ctgatccggg
       61 ccaacacgga agagctggcg cagatcctga cggcggaaca gggcaagccg ctggccgagt
      121 cgcgcggcga ggtcggcatg agcgcggcct atgtgcagtg gttcgccgag gaggcccggc
      181 gcatctacgg cgacaccatc ccttcgccct ggggcgaccg gcgcattgtc gtcaccaagg
      241 agccgatcgg cgtggtggcg gcgatcacgc cctggaactt cccctcctcc atgatcgcgc
      301 gcaagctcgg cgccgcactg gcggcgggct gcaccattgt tttcaagccg gcggaattga
      361 cgccgtactc aggacttgcc tggggcgtcc tgtgcgagaa ggccggggtt ccggccggcg
      421 tcgccaactt catcaccggc acgccggcgg aaatcggggc ggaattcacc gccaatccgg
      481 tggtgcgcaa gatcaccttt accggctcga ccgccgtcgg caagctcctg atgaagcaaa
      541 gcgccgacac gatgaagcgg atttcgctgg aactcggcgg ccatgcgccc ttcatcgtct
      601 tcgatgatgc ggatgttgat ctggcggtcg agcaggccat cgcctcgaag taccgcaaca
      661 ccgggcagac ctgcgtctgc accaaccgct tcattgtgca ggacggcgtg tacgcggcgt
      721 tcgccgacag gttcgcggct gcggtcagga ctctcaaggt cggcgcaggc accgagcagg
      781 gcgtggcgca gggaccgctg atcagcgagg ccgctctggc gaaggtcgaa agtcatgtcg
      841 aggatgcggc tcgcaagggc ggcaagctgc tttccggcgg caagcgccat gcgctcggcc
      901 gcaccttcta tgaaccaacc gtcatcgccg aggcgacgcg ggacatgctg atcgccagcg
      961 aggagacctt cgggccggtg gcaccgctgt tccgcttcaa ggacgaggcg gaggctgtcg
     1021 cgctggcgaa cgacacgcct tacgggcttg ccggctattt cttcacccgc gatcttgccc
     1081 gcgcgttccg cgtcgccgaa gcgctgcagt atggacaggt cggcatcaac gctggcgtca
     1141 tcacctccga agtggcgccc ttcggcggcg tgaaggaatc cggcatgggc cgcgaaggct
     1201 ccaagtacgg cctcgacgag tatttgagcg tcaagtacct gtgcttcggc ggcctgcgct
     1261 gagggactgg cgccgcccta ttcctgcacc tcagcgtatt cgcggcgctg cgccgcgagt
     1321 gccctgagct gggcttggag catcgtctga tccgattgga caaggctcta cggaatccgg
     1381 gctgcccatt tcatccagag cccacgccac aattgccctt cgctgcatga tgtcatacaa
     1441 ctagcctgac aagcacgggc caacgcatga ctgaccgaga ccccaagaaa ctccgcagcc
     1501 accgatggtt cggcacggac gatatccgcg ccttcgggca ccgctcccgc atggtccagg
     1561 ccggacttgc ccacgaggaa ttcgttggca ggccctgcat cggtatcatc aatacctggt
     1621 cggacatcaa cacctgccac acgcatctgc gcgagcgggc gcaggaggtc cggcgcggcg
     1681 tgctgcaagc aggcggattt cccgtggaac tgccggcgct ttccttgtcc gagcaatatg
     1741 tcaagccgac aacgatgctt taccgcaacc tcctggcgat ggagacggaa gagctgatcc
     1801 gctgccaccc gctcgacggc gtggtgctgc tcggtggctg cgacaagacc acgccggcgc
     1861 tgctgatggg cgcggcgagc gccaacgtcc cggcgattta cgttccggcg ggagcgatgc
     1921 tgcggggcaa ttatcgaggc cagatcctag gttccggctc cgacgtatgg aagtactggc
     1981 atgagaagga agcaggaaac atatccaagg cgcagtggga ttcgatggaa ctgggcatcg
     2041 cccgttcctt cggcacctgc atgacggccg gcacggcggc caccatgatg tcgattgctg
     2101 acgcgctggg catgaccctg cctggagcgt cgtctattcc ggctgcggat gcagcccatc
     2161 cccgcatggc ggcggcctgc ggacggcgca tcgtcgacat ggtctgggaa ggcctgacac
     2221 cgcgcaaggt gctcacgcgc gccgcgttcg aaaatgccct ggtcacgcac atggcgcttg
     2281 ccggctcgac gaacgccatc atccacctcg tggccatggc ggggcgtgcc ggcgtgaagg
     2341 tcgaccttgc cgatttcgag acctattccc gccgcgtccc ggttatcgca aatctgcgac
     2401 cgtccggcgc ctacctgatg gaggatttct tctatgccgg cgggcttccg ggcttcctga
     2461 acgtgctgaa gccgttcctc aacctcgacg ccatgaccgt taccggcaag acgctgggcg
     2521 acaacatcga cggcgcggaa gtgttcaatc aggatgttat tcgaccgctc gacaagccga
     2581 tcgccgcttc gggcggcacg gccgtgctct acggcaacct cgcgcccaag ggttgcgtga
     2641 tgaagccgcc ggcagccgag gcccgcctgt tgaaacacac cggtccggcc atcgtctttg
     2701 acacctacga ggcgatgaag aaggccgttg acgacgaggg cctggacgtg acgaaggacc
     2761 acgtgatggt gttccgcaac gcggggccgg tcggcgggcc cggcatgccg gagtggggca
     2821 tgctgcctct gccgaagaag ctcctgaagc agggcgtgcg cgacatgatc cgcatctccg
     2881 atgcacgcat gtccggcacc tcctatggcg cctgcattct gcacgtttcg ccggaagcct
     2941 atgtcggcgg cccgctcgcg gcggtgcgca acggcgatct gatctcgatc gatgtcgagg
     3001 cgcgaaccat ccacctccat gtggacgatg ccgaaatcgc ccgccgtgtc gccgcatacg
     3061 agcgtccgga ccgcgatttc ccgcgcggat acggcaagat gtatgccgcc catatcaagc
     3121 aggcccatga tggctgcgac ttcgatttcc tcgaaggcac gcagccgatc cccgaacccg
     3181 ccatcttctg aaccgtgcat cggaaaggcg atcgatgaaa tcccgcaggg tctgtgtcga
     3241 ggatctcgga cgcccgttgc tggcggcgct gccgctggcc cgcgccggaa agccgcgggc
     3301 cgcagtgctg acggcggagc accttcgcca acaacgctcg ccgcagcaaa ggcggcatgt
     3361 ggcgatgcgg ctcgccggcg tggccgacac cggatcgagg tatccgatca tcaccgatat
     3421 cgagcacgcg acaatactgg ctgccatcaa tgccggagcg ttgacgctgc gcgacgagaa
     3481 cggccgcatg ctggccagga gcaccgaatg actcgcaaac cacaacgcct gctgatcacc
     3541 ggagccgccg gtatgctcgg caaacagctt cggcggcgcc tggccggcga gtacgcgctg
     3601 ctgcgtctgt ccgacgtcgc ctcgatggaa cctgccggag ccggcgagga aacggtcgtt
     3661 tgcgatctcg ccgatcggga gggcgtcggc cgcctgtgcg agggcatcga cgccatcgtg
     3721 catctgggtg gacgggcaac cgaggccgat tgggacacag tccattccgc caacatcctg
     3781 ggcgccatcc atctctacga gggtgcccgc aaggcgaagg tggagcgggt gctgttcgct
     3841 tcctccaacc acgccgtcgg gctcgtccgg cgtactccga agacagacca tactgcttcg
     3901 gcgcgtcccg acagccgtta tgggctttcc aaggccttcg gtgaggacat cgcggcgctt
     3961 tatgcctaca agcatggcgt ccgtgcctat tgcatgcgca tcggctcgtg ctttcccgag
     4021 ccgaccgacg agcgcatgct gtcgagctgg ctgtcctacg acgatttcga gcgcctggtg
     4081 cgcaccggac tcgccgccga ctatacctac gaaatcgtct atggcatctc gcgcaacacc
     4141 cgcgcctggt gggacaattc caatgcctac cggctgggct acaacccgca ggacaatgcg
     4201 gaagccttcg cccccagatt gaccggggtc ggggtcggca atccgctgca tgatcctttc
     4261 atcggcggtg gctacgttag cccggatttc gaggctgatc cttggaagat tccgtagcgt
     4321 cgcgtcaccg acggcttgat gccgtgcgcg gtatccaaca ttcggcggcg gagccggata
     4381 ccacgacgca gacagggcgg aaaccgaaac gggaggttgg gatgaacgag acggcgaagt
     4441 ccctggggcc ggtcagcgga acccagagcc gcaccggcga tctggtccgc atgctttccg
     4501 aggatatccg ctcaggccgc atcaggccgg gagagaaact gccgaccgaa caggaaatcg
     4561 tcagaatgac cggcgtcagc cgaaccgttg tgcgcgaagc ggtcgcggca ctgcgggcgg
     4621 aagggctggt ggtcacacgc cagggcgtcg gcgccttcgt cacggcggcg ccgggccagg
     4681 gcatgttcaa gatcagcgcg gaggaagccg gatcgctcga gcgcgtcctg cagatcctgg
     4741 aactgcgcat cggcatggaa gtcgaggcgg ccggactcgc tgccgaacgc gccgacaggg
     4801 aggctgtcgc ccgcatcgcc ggccatcagg ccgagttcaa cgccggcgca cagaacggcg
     4861 acatcgtcat cggcgccgac tatggctttc atcgcgcggt cagcgaggcc acgcgaaacc
     4921 cctactttcc gcgcatcctg aactcgctcg gccagatcct cattccgcgt ggcaacgtgc
     4981 cttcgagcct gccaacgaag gaggagcggc gcagctacct gatgcgtatc gccggggagc
     5041 acgaggcaat tctcgccgcg attcgcaagc gcgatgtcga aggcgcgcgc tcagccatgc
     5101 gtcaccatct cgtcgcttcc cgagaacgct accagctgct cgccgaaaag tcctgaccgg
     5161 agccgggggc acttgaacga gcgctgcgag cctcaaaaga ggctgccctg gccgcccgac
     5221 ggcgccgccg gctgtttggc gcgggacttt ggccgcgcgc cctcgccagc ggtcgtcgcc
     5281 acctccccgt ccgcgaattc gatgcgcaga gccatgccct cgctgaccgc cgccgcgtgc
     5341 cgcactgcct tgccatcgcc gtcgcgcacc agggcaaagc cgcgcgccag cacctgacga
     5401 tagccgagcg agcccagcag tgagtccagc cggtgcagcc tggcggcgcg atcggcctgt
     5461 cgcatgccca atgctctcgg aagccgctcc gccagcgcca caagccgctc gcgccggcgc
     5521 agcatgtcac gccgttcgcc ggccaaacgt gcggcaaagg cgacggccag gctcttacca
     5581 accgcatcaa gccgctgccg gcgcagttcc gtccttcgcg ccagtgatcg ttcgagcagc
     5641 gcgccaaggc cgctgattcg ctcggcgaac cgggcgagtc tggcctgcgg cgcctgggcc
     5701 gccagccggc gcgccagaac gccgatcagg ccattgcgcc gctctactcc ggaacgcacc
     5761 gcaccggaaa gcttgagcga ggtcacgtcc agccgctggc gcggtccggc cagcagggct
     5821 tcgggacccg gcagc
//
LOCUS       DAOMXN010000167         5820 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_7296_length_5820_cov_11.425846, whole genome shotgun sequence.
ACCESSION   DAOMXN010000167 DAOMXN010000000
VERSION     DAOMXN010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 5820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5820
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7296_length_5820_cov_11.425846"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="PK812_10860"
     CDS             complement(<1..184)
                     /locus_tag="PK812_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70098.1"
                     /translation="MSPSLRHTLYLASALAIVFPLMIAPSQAQNANLGGGFIEFLVTG
                     RTGAAPRHAPPTQPAHS"
     gene            complement(438..1844)
                     /locus_tag="PK812_10865"
     CDS             complement(438..1844)
                     /locus_tag="PK812_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HRJ70099.1"
                     /translation="MLAIITFSVNAIANFALGLGVAWFLGAEQFGVYALIVAGGAVLQ
                     TLLFEWLRLSANRFFGKRQAEADPNLYTTLSLGTGVLSIALSVLAIVLYITDGAFGVA
                     ATVAALAPLTGMAGGLFDFRCAIARAQFDNRRYAMMVIAKNLLALLLMIGGAFWLKRA
                     DVVVAGLCVSSVAGALVTARAFREGHAPGRFDRLLVRQFAIYAGPMIVSSLIYQLNMF
                     MTRSGVAFHYGLAESGRYSLALDIGLKLTTTVGSALDILLFQLAVRAESEESREAATA
                     QLSRNLLIVTGILAPACVGYWLILPSFEALFVAESYRAAFDAYTLYLLPGLFAVSLVF
                     YAINPQFQIARKTWPMLLAALANIAVAAAVLYLWPGTDGRHGALATSSGFVVGGLLMV
                     VIAWRTAPIDVPWLDLGKVVAATALMSAAALPARALEPGAFTLTLTALAGGAAYGLLA
                     LALNIGGLRTLLIARLRR"
     gene            1970..2809
                     /locus_tag="PK812_10870"
     CDS             1970..2809
                     /locus_tag="PK812_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRJ70100.1"
                     /translation="MRAKPILLALAAIGLFAVMNVLIKHLSFYYDTLQVAFVRYLSAG
                     LVALLWVWRMRPGWPAIDVVEGNVLRGVLSLATGLSFFYALALLPLADAIALSFLAPV
                     FMMIFSSLFLKERPGLLGLLAIAFGFAGMLVMLWGKLESDGRSLWGVSASVFSAVTYA
                     LSMILLRSRAQRDPVALIVFFQSWIPALLIAPMAGTAWEPLAYQDIAPFAALGVLGVT
                     AHLLMATAYSRASAATLAPIEYSALIWAALLGFFIFAEMPTWRTLAGAALIVAGSLLT
                     SRR"
     gene            3005..3292
                     /gene="groES"
                     /locus_tag="PK812_10875"
     CDS             3005..3292
                     /gene="groES"
                     /locus_tag="PK812_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HRJ70101.1"
                     /translation="MKFRPLHDRVVVRRLNSEEKTKGGIIIPDTAKEKPQEGEIIAVG
                     TGARDENGKLVPLDVKKGDKVLFGKWSGTEVKIDGEELLIMKESDIMGVIG"
     gene            3361..4998
                     /gene="groL"
                     /locus_tag="PK812_10880"
     CDS             3361..4998
                     /gene="groL"
                     /locus_tag="PK812_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010682003.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HRJ70102.1"
                     /translation="MAAKDVRFSSDARERMLRGVDILANAVKVTLGPKGRNVVIDKSF
                     GAPRITKDGVTVAKEIELADKFENMGAQMVREVASKTNDVAGDGTTTATVLAQAIVRE
                     GAKYVAAGMNPMDLKRGIDLATAAAVDDIKKRSKKVKDSSEVAQVGTISANGDKQIGE
                     MIANAMQKVGNEGVITVEEAKTADTDVDIVEGMQFDRGYLSPYFVTNAEKMVAELEDA
                     YLLIFEKKLSSLQPMLPILESVVQTGKPLVIIAEDVEGEALATLVVNKLRGGLKVAAV
                     KAPGFGDRRKAMLEDIAILTGGSMIAEDLGIKLENVSLNMLGRAKRVRIEKENTTIID
                     GAGKKKDITDRVAQIKAQIEETTSDYDREKLQERLAKLAGGVAVIRVGGATEVEVKEK
                     KDRVDDALNATRAAVEEGIVPGGGCALLRAKAAVEKVKSENADIAAGIQIVLKALEAP
                     IRQIVENAGCEGSIVVGKIGENKSATFGFNAQTEEYVDMIQAGIVDPAKVVRTALQDA
                     ASVAGLLITTEAMVAEAPKKDAPAPAMPGGGMGGMDF"
     gene            complement(5189..>5820)
                     /locus_tag="PK812_10885"
     CDS             complement(5189..>5820)
                     /locus_tag="PK812_10885"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HRJ70103.1"
                     /translation="GDIAVRNVADIYVFPNTLKIVKITGAELKDWLERSAGIFNQLKA
                     SDDEQLLVNLAFPPYNFDVIDGVTYEIDVSQPSKFDGDGKLIAEGANRIVNLKLNGQP
                     VKPDDTVAVVSNNYRASGGGTFAGAVSKNIIYDSPDYSRDIVASYLMQAGTVSPKADG
                     NWKLAPFPAAKHVVLESSPLARDLLSTVPGVTHVGDGQNGFSRFKVPMG"
BASE COUNT         1083 a         1845 c         1922 g          970 t
ORIGIN      
        1 cggagtgcgc cggctgggtc ggcggcgcat ggcgcggcgc ggcgccggtc cgtccggtga
       61 ccaggaattc gatgaagccg ccgccaagat tggcgttctg cgcctggctc ggcgcaatca
      121 tcaggggaaa gacaatcgcc agggcactgg cgaggtaaag ggtgtgacgc aggctagggg
      181 acatgacgca actctcgttc cggctggcat ccggggcgcg gatgcgggtg aaccggagaa
      241 cgagctaaga gcgcaaacag atcagaaagg ttaatcgtgc agcttgcttt aatgaattga
      301 tattatattt accatgaaaa tcgagttatt gtaattgata gcctaaagtg catagtaaag
      361 gatgcatttc ttgttaagga taacgatttg ttacagggtc ccgcagagcg gttcggccct
      421 gcggtccaga cctcgctcta gcgccggagc cgagcgatga ggagggtgcg cagcccgccg
      481 atgttcagcg ccagcgccag cagtccatag gccgcgccgc cggccagggc cgtgagcgtc
      541 agtgtgaagg ccccgggctc cagcgcgcgc gctggcagcg ccgcggcgct catcagcgct
      601 gttgcggcca ccaccttgcc aaggtcgagc cagggaacgt cgatcggtgc ggtccgccag
      661 gcgatcacca ccatcagcag gccgccgaca acgaagcccg agcttgtcgc cagcgcgccg
      721 tgacggccat ccgttccggg ccagaggtag agcacggcgg ccgccacggc gatgttggcg
      781 agcgccgcca gcagcatcgg ccaggtcttc cgggcgatct ggaactgcgg gttgatggcg
      841 tagaagacca ggctgaccgc gaacaggccg ggcagcagat acagggtgta ggcatcgaag
      901 gcggcccgat agctttccgc cacgaacagc gcctcgaagc tcggcaggat cagccagtag
      961 ccgacgcagg ccggcgccag gatgcccgtg acgatcagca ggttgcgact gagttgcgcc
     1021 gtggccgcct cgcggctttc ctcgctctcg gcccgcacgg cgagctggaa cagcaggatg
     1081 tcgagcgccg atccgacggt ggtggtcagc ttcaggccga tgtcgagcgc gagcgagtaa
     1141 cgtccgcttt ccgccagccc gtagtgaaat gccacgcctg atcgcgtcat gaacatgttg
     1201 agctggtaga tcagcgagga cacgatcatc ggaccggcat agatcgcgaa ctgccggacc
     1261 agcaggcggt cgaagcggcc gggggcgtgc ccttcgcgaa aggcgcgcgc cgtcaccagg
     1321 gcgccggcga cggaactgac gcagagtccg gcgacgacca catcggcccg cttcagccag
     1381 aaggcgccgc cgatcatcag cagcagcgcc agcaggttct tggcgatcac catcatcgca
     1441 tagcggcgat tgtcgaactg cgcgcgcgcg atggcgcagc gaaagtcgaa caggccgccg
     1501 gccatgccgg tcaggggcgc gagcgccgcg accgtcgccg cgacgccgaa cgcgccatcg
     1561 gtgatataca ggacgatcgc cagcacgctc agggcgatgg agagcacgcc ggtgccgagg
     1621 ctgagcgttg tatagaggtt cgggtcggcc tcggcctgcc tcttgccgaa aaagcggttc
     1681 gccgacaggc gcagccactc gaacagcagg gtctggagca ccgcgccgcc ggcgacgatc
     1741 agggcataga cgccgaactg ctcggcgccg agaaaccacg cgacgccgag gccaagagcg
     1801 aaattggcga tggcgttgac gctgaaggtg atgatcgcaa gcatggccgg tcccgcgcct
     1861 gccatagcct atccgcgata aagaacgctt aagcggcgga aattcagacc gccttgcgtg
     1921 ccggcgcgcc tgtgtctaag cgggtactct gacctgatcc ggaacccccg tgcgcgccaa
     1981 accgatcctg ctggccctcg ccgccatcgg cctgtttgcc gtgatgaacg tgctgatcaa
     2041 gcatctgtcg ttctactacg acacgctgca ggtggccttc gtgcgctatc tcagcgccgg
     2101 gcttgtcgcg ctgctctggg tctggcgtat gcgtcccggc tggcccgcca tcgacgtagt
     2161 cgagggcaac gtgctgcgcg gcgtgctcag cctggcgacg ggcctgtcgt ttttctacgc
     2221 gttggccctg ctgcccctgg ccgacgccat cgcgctcagc tttctcgcgc cggtcttcat
     2281 gatgatcttc tcctcgctct tcctgaagga gcgtccgggc ctgctcggcc tcctggccat
     2341 cgcgttcggc ttcgccggca tgctggtcat gctgtgggga aagctggagt ccgacgggcg
     2401 gtcgctgtgg ggcgtctccg cctcggtctt ctcggccgtc acctatgccc tgtcgatgat
     2461 cctgctgcgc agccgcgccc agcgcgatcc ggtcgcgctg atcgttttct tccagagctg
     2521 gattccggcc ctgctgatcg cgccgatggc cgggacggcc tgggagccgc tggcctacca
     2581 ggacatcgcg cccttcgccg cgctgggcgt gctcggcgtc acggcccatc tgctgatggc
     2641 cacggcctat tcccgggctt cggccgcgac cctggcgccg atcgagtact ccgcgctgat
     2701 ctgggcggcg ctgctcggct tcttcatctt tgccgagatg ccgacctggc gcaccctcgc
     2761 cggcgcggcc ctgatcgtcg cggggtcgct gctgacatcg cggcgctgac cgtcaaccgg
     2821 gggttaacca tgccggacat ggtgaatctt ttcctgccgt cggttcttga ctccgaaaac
     2881 gcgcgcacct acatccgcga cgttagtagc actccctgaa ggagagtgct aacagtcgcg
     2941 cctccgagag ggcgactgtc acaaggacaa actgaacccg tttcagtcaa gaggaagaga
     3001 acccatgaag tttcgtccgt tgcacgaccg cgtcgtcgtc cgtcgcctga acagcgagga
     3061 aaagacgaag ggcggcatca tcattccgga taccgccaag gagaagccgc aggagggcga
     3121 gatcatcgcc gtcggcaccg gcgcccgcga tgagaatggc aagctcgtgc cgctcgatgt
     3181 caagaagggc gacaaggtgc tgttcggcaa gtggtccggc accgaagtga agatcgatgg
     3241 cgaggaactc ctgatcatga aggagtccga catcatgggc gtgatcggct gatcgcgcga
     3301 caggcgaatg gccgccggcc gttcgcatcc ttgtaaacct cacatcagga atttcagaca
     3361 atggccgcta aagacgtccg cttttcgtcc gatgctcgcg agcgcatgct gcgcggcgtg
     3421 gacattctcg ccaatgccgt gaaggtcacg ctgggtccca agggccgcaa tgtggtgatc
     3481 gacaagtcgt tcggcgcccc gcgcatcacc aaggacggcg tcaccgtcgc caaggagatc
     3541 gagctcgccg acaagttcga gaacatgggc gcgcagatgg tccgcgaggt cgcctcgaag
     3601 accaatgacg tggctggcga cggcaccacc accgccaccg ttctcgccca ggcgatcgtg
     3661 cgtgaaggcg ccaagtacgt ggccgccggc atgaacccga tggacctgaa gcgcggcatc
     3721 gacctcgcca ccgccgccgc ggtcgacgac atcaagaagc gctcgaagaa ggtgaaggac
     3781 tcgtccgaag tggcgcaggt cggcaccatc tccgccaacg gggacaagca gatcggcgag
     3841 atgatcgcca atgccatgca gaaggttggc aacgagggcg tcatcacggt cgaggaagcc
     3901 aagaccgccg acaccgacgt cgacatcgtc gagggcatgc agttcgaccg cggctatctc
     3961 tcgccgtact tcgtcactaa cgccgagaag atggtggccg agctggaaga cgcctatctc
     4021 ctcattttcg agaagaagct ctcctcgctg cagccgatgc tgccgatcct ggaaagcgtg
     4081 gtgcagaccg gcaagccgct ggtcatcatc gccgaggacg tcgagggcga ggctctggcc
     4141 acgctcgtgg tcaacaagct gcgcggcggc ctgaaggtcg cggcggtgaa ggcccccggc
     4201 ttcggtgatc gccgcaaggc catgctcgag gacatcgcaa tcctcaccgg cggctcgatg
     4261 atcgccgaag acctcggcat caagctcgag aacgtgtccc tcaacatgct cggccgcgcc
     4321 aagcgggtcc gcatcgagaa ggagaacacc acgatcatcg acggcgccgg caagaagaag
     4381 gacatcaccg accgtgttgc ccagatcaag gcgcagatcg aggagaccac ctcggactac
     4441 gaccgcgaga agctccagga gcgtctggcg aagctggcgg gcggcgtcgc ggtgatccgc
     4501 gtcggcggcg ccaccgaggt cgaggtgaag gagaagaagg accgcgtgga cgacgcgctg
     4561 aacgccaccc gcgccgcggt ggaagagggc atcgttccgg gcggcggctg cgccctgctg
     4621 cgcgccaagg ccgctgtcga gaaggtgaag tcggagaacg ccgacatcgc cgccggcatc
     4681 cagatcgtcc tcaaggcgct tgaggctccg atccgccaga tcgtcgagaa cgcgggctgc
     4741 gaaggctcga tcgtcgtcgg caagatcggc gagaacaagt cggccacctt cggcttcaac
     4801 gcccagaccg aagagtatgt ggacatgatc caggccggca tcgtcgatcc ggccaaggtc
     4861 gtccgcaccg ctctgcagga cgcggcttcg gtcgccggcc tgctcatcac caccgaggcg
     4921 atggttgcgg aagccccgaa gaaggacgcc cccgctccgg ccatgccggg cggcggcatg
     4981 ggcggcatgg acttctaaga gaagtccagg atcgccctct tcacccaagt gttccgaaac
     5041 cgggcaggcc cggtttcggt ggtggcatgg acttctgatt gaagtccgca catccttccg
     5101 agacaagaaa gggcgccgcg aggcgccctt tttttgttct gccttcatca gccggagcgg
     5161 atccggtgcc gttgagcgcg gcgcgcgctc accccatcgg caccttgaac cgggaaaagc
     5221 cattctgccc gtcgccgaca tgcgtgacgc ccggtacggt cgaaagcagg tcccgggcca
     5281 gcgggctcga ttccagcacc acatgcttcg ccgctgggaa gggcgcgagc ttccagttgc
     5341 cgtccgcctt gggcgatacg gttcccgcct gcatcaggta gcttgccacg atgtcgcggg
     5401 aatagtcggg ggaatcgtag atgatgttct tcgacaccgc ccccgcgaag gttcccccgc
     5461 cggaagcgcg atagttgttg gagacgacgg cgacggtgtc gtccggcttc accggctggc
     5521 cgttcagctt caggttgacg atgcggttcg cgccctcggc gatcagcttg ccgtcgccat
     5581 cgaacttcga gggctgtgag acgtcgatct cgtaggtcac gccgtcgatc acatcgaaat
     5641 tgtagggcgg gaaggcgagg ttcacgagca gctgctcgtc gtcactggcc ttgagctggt
     5701 tgaagatgcc ggccgagcgc tccagccagt ccttcaattc ggcgccggtg atcttcacga
     5761 tcttcagcgt gttgggaaag acgtagatgt ccgcgacatt gcgcacggcg atgtcgcccg
//
LOCUS       DAOMXN010000168         5797 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_7329_length_5797_cov_8.833159, whole genome shotgun sequence.
ACCESSION   DAOMXN010000168 DAOMXN010000000
VERSION     DAOMXN010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 5797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5797
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7329_length_5797_cov_8.833159"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="PK812_10890"
     CDS             complement(<1..559)
                     /locus_tag="PK812_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007765933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRJ70104.1"
                     /translation="MSPRTSRRSVVLGLVAMPSLVRAQSAWPAQIIKFVVPFAPGGST
                     DILARVVGQKLEAPLKASILIENRPGAGGSVAANAVAKAVPDGHTFMMGHIGTLAFTP
                     SLYPNLPYDPVKDFAPVALVATVPNILAVHPSVPARSFAEFVAHAKANPGKLSYGSGG
                     IGSAAHVAGAYLASAAGFDALHVPYR"
     gene            complement(556..1386)
                     /locus_tag="PK812_10895"
     CDS             complement(556..1386)
                     /locus_tag="PK812_10895"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HRJ70105.1"
                     /translation="MSAPNTRHIAFIGFGEVGQTFAKALSTHDGTLLTAHDILVGTGS
                     DRGMAAKAQAIGVRLFDTPEAAVRAAEVVISAVTASEAESVAANAARYLTPGQLFLDV
                     NSAAPSTKQRAAAHVAGTGAHYVECAVMAPVLKPGLAVPILAGGPHAAEAAERMNRLG
                     MNLTPVAVDYGLASAMKLCRSIMIKGIEALMVDCTAACDAWGVKEPVFASLAETFPSI
                     DWHALALNMRERVATHGVRRAAEMREAGEMLEALGMNPDLANAVAAAQARGAQPGRTS
                     "
     gene            complement(1477..2406)
                     /locus_tag="PK812_10900"
     CDS             complement(1477..2406)
                     /locus_tag="PK812_10900"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRJ70106.1"
                     /translation="MRIDMLGLEAFIAIAERGSFHAAASHLNLSQTALSHRIRKLEES
                     LGAELLTRTTRQVSLTEAGATLLPRARRLFDDLDAALDDVRSQASDMQERVVIGCLPT
                     IAMQCMPEVIADFALRRPNVAVKLLDSSASDIAEKVQSGEAEFGITIVAANRWNLDLK
                     AIAKEPFVLVCSRKLPIAANASLTWAEIGAVPLVRISAETGNRILIDDALGTRRETLN
                     WRYEVQRVTTAISLVRSGIGGAIVPQLGVSIAVAGAQDLVAIPIRGPLVTRTLGLVTR
                     KGAPLTPAARELRKLVIAALKASFARGGFSDIS"
     gene            complement(2626..2700)
                     /locus_tag="PK812_10905"
     tRNA            complement(2626..2700)
                     /locus_tag="PK812_10905"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2666..2668),aa:Lys,seq:ttt)
     gene            complement(2795..3238)
                     /gene="lysM"
                     /locus_tag="PK812_10910"
     CDS             complement(2795..3238)
                     /gene="lysM"
                     /locus_tag="PK812_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HRJ70107.1"
                     /translation="MGLFSFAKSVGAKLFGASEAKAATADELKKEVEKHGLKADGLSI
                     SVDGDKVKVSGNAMSQADAEKIILALGNTIGVAQVDSALIAKQSAPESRMYTVVKGDN
                     LWKIAEAMYGKGKGAEHERIFEANKPMLTHPDKIYPGQVLRIPPL"
     gene            complement(3378..5537)
                     /gene="mdoH"
                     /locus_tag="PK812_10915"
     CDS             complement(3378..5537)
                     /gene="mdoH"
                     /locus_tag="PK812_10915"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HRJ70108.1"
                     /translation="MQEGRRHDQRTHPAMPNEVPLAMPVQDFRRWTEAERRAPVARDA
                     FAPPWVVRLFVFGGGLVLTGYGAWQMRRVVDAGSGVTFLQWVLLALFVINFVWIALAF
                     TGAITGFLSLLLRRPGRLTVPRTLSTRTAVVMPIYNEAPSRVFAAMQTMAEGVAATGL
                     GQHFDFFFLSDTTDPDAWIGEERAYFALREKFPAEIALYYRHRIKNTGRKAGNIADFV
                     SRWGGAYEHMLVLDADSLVDGDTIVALAGLMEADPDSGIIQTLPLIINRNTLFARLQQ
                     FAARVYGPVIAEGLRLWMGSDGNYWGHNAIIRTRAFADHCAMPELKGKPPFGGHILSH
                     DFVEAALIRRAGWKVTMVPGLVGSYEESPPSLIDLAARDRRWCQGNLQHSRILFARGF
                     VPATRHHFLTGIVSYLASPFWLMQLAVGIVLVLQAFFIRPEYFTSEFSLFPAWPRFDA
                     ERALNLFVVTMGILLAPKLFGVILALTRPEDRRAHGGGILLILSALLEIIMSALIAPI
                     MMIIQSGAVFQILFGGDTGWKPQRRDDGSIPLRDIVRRHRMHVTFGVLAGIITWLISP
                     SLFLWMSPTLVGLVLAILISWATGLVGLGLALRRLGILWTPEERSPPAIATRANALQA
                     HYGDFDEVNGVLALYRDERLRDRHMAMLPAHAPRRRGEIDRDHVVAEAKIADAETVAD
                     AAAWLSNREKAITFQDRALVAMLARLAPGKGERTATS"
     gene            complement(5543..>5797)
                     /locus_tag="PK812_10920"
     CDS             complement(5543..>5797)
                     /locus_tag="PK812_10920"
                     /inference="COORDINATES: protein motif:HMM:NF023169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HRJ70109.1"
                     /translation="RRRRFVVDFAGDKLAAAKPPEIVPNLWASNNGISNVRLIPGAGG
                     KVTRVVFDLDTGNESLIELRLNLKNGDTPLTETWLYRWTS"
BASE COUNT         1007 a         1901 c         1952 g          937 t
ORIGIN      
        1 cccggtaggg cacgtgaagc gcgtcgaaac ctgcggccga ggcgagatag gctcccgcca
       61 catgcgccgc gctgccgatc ccgccggagc cataggagag cttgcccggg ttcgccttcg
      121 catgggccac gaattcggcg aaactcctgg caggcaccga agggtgcacc gccaggatgt
      181 tcggcaccgt ggccaccagt gccacgggcg cgaagtcctt caccgggtcg tagggcaggt
      241 tcggatagag cgagggggtg aaggccagtg tgccgatatg ccccatcatg aacgtatggc
      301 cgtcggggac ggccttggcg accgcattgg ccgcgacaga gccgcccgcg cccggccggt
      361 tctcgataag gatgctggcc ttgagcggcg cttccagctt ctgtcccacg acccgggcga
      421 gaatgtccgt gctgccgccc ggcgcgaaag gcacgacgaa tttgatgatc tgcgcgggcc
      481 aggcggactg ggcgcggacc agcgagggca ttgccacaag gcccaggaca acgctccgtc
      541 tgcttgtgcg tgggctcatg acgtcctccc gggttgggcg ccgcgcgcct gcgcggcggc
      601 caccgcattg gcgaggtccg gattcatgcc gagcgcttcg agcatttcgc ccgcctcgcg
      661 catttccgcc gcgcggcgga cgccgtgcgt tgcgacccgc tcgcgcatgt tgagggcgag
      721 agcatgccag tcaattgacg gaaacgtctc ggcgagactg gcgaacaccg gctccttcac
      781 gccccacgcg tcgcaggctg cggtgcagtc caccatcagg gcctcgatgc ccttgatcat
      841 gatgctgcgg cagagcttca tcgccgatgc cagaccgtag tcgacagcca ccggggtgag
      901 gttcatgccc agccgattca tccgctcggc cgcctcggcg gcatgcggac cgccggcgag
      961 gattggcacc gcgagccccg gcttcagcac cggcgccatg acggcgcatt ccacgtagtg
     1021 cgcccctgtg ccggcgacat gtgccgccgc ccgttgcttc gttgacgggg cggccgaatt
     1081 gacgtcgaga aacaactgcc ccggcgtgag gtagcgtgcc gcgttggcgg cgacgctttc
     1141 cgcctccgag gcggtgacgg ctgaaatgac gacttcggcg gcgcgaaccg ccgcctcggg
     1201 cgtatcgaac agcctgacgc caatggcctg ggccttggcc gccatgcccc ggtccgatcc
     1261 ggtgcctaca aggatgtcat gggccgtcag gagcgtgccg tcatgggtcg agagggcctt
     1321 ggcgaaggtc tggccgacct cgccgaagcc gatgaaagcg atgtggcgcg tgttgggtgc
     1381 cgacatgacg ggcgcaagcc tctctcaggg atgaaagcta tcgcgcgtcg cattatacga
     1441 tcaaatgatc gctttgcagg aattcatgaa caagtttcac gaaatgtcgg aaaagccccc
     1501 ccgcgcgaaa ctcgccttga gcgccgcgat gacgagtttt cgcagttctc gcgcagcggg
     1561 cgtcaacggc gcccctttcc gcgtcacgag gccgagcgta cgcgtgacca gaggaccacg
     1621 gatcggaatc gccacaaggt cctgcgctcc ggcaacagca atcgagacgc cgagctgcgg
     1681 cacaatggcg ccgccgatcc cggaccgaac gaggctgatc gcggtcgtga cgcgctgcac
     1741 ctcatagcgc cagttgagtg tttcgcggcg ggtgccgagg gcgtcgtcga tcaggatgcg
     1801 gttgccggtc tcggcggaga tgcggaccag cggaacggcg ccgatctccg cccaggtgag
     1861 cgaggcattg gccgcgatcg gcagcttgcg gctgcacacc aggacgaaag gctccttggc
     1921 gatcgccttc aggtcgagat tccagcgatt ggccgcgacg atcgtgatcc cgaattccgc
     1981 ctcgcccgac tgcacctttt cggcgatgtc gctggccgaa ctgtcaagca gcttcacggc
     2041 gacattgggt cggcgcaagg cgaagtcggc gatgacttcg ggcatgcact gcatggcgat
     2101 ggtcggcagg catccgatca ccacgcgctc ctgcatgtca gaagcctggc tccgcacgtc
     2161 gtccagcgca gcgtcgagat cgtcgaacaa ccggcgcgcc ctcggcagca gcgtcgctcc
     2221 cgcctcggtc agcgagacct gccgcgtcgt ccgcgtcaga agttcagcgc cgaggctctc
     2281 ctccagcttg cgaatccgat ggctcaacgc tgtctgcgac aggttgagat gcgaggcggc
     2341 cgcgtggaag ctgccgcgtt cggcaatcgc gatgaaggct tccagtccca gcatgtcgat
     2401 gcgcatgaca gctcccgcga gagccagcgc ggctcagcag cgcaagccta tcaagcgccg
     2461 gcgggccccg aaagccggca tccgcggtgc aaccggcatc tacgtgtccg actctggtct
     2521 ggaaccgaag cgccccgtgt gcccgcagcg gtcaacgcgc cgaaatccac tggaccgcgc
     2581 caaagcatgt ccgtgagtca ggatcgggct acacaggaac gatgatggtg agcggggagg
     2641 ggatcgaacc ctcgaccatc tgattaaaag tcagatgctc taccactgag ctacccgccc
     2701 gcgccggtcg gcgggagatt ccccaagcca aacgaaacgc gcccgctgtc tagaagaacg
     2761 gcgggcgcgg gtcaattcgt gacggcggcg tggtttacag cggcggaatg cgcagcacct
     2821 ggcccgggta gatcttgtcc ggatgcgtca gcatcggctt gttggcctcg aagatgcgct
     2881 catgctcggc gcccttgccc ttgccataca tggcttcggc gatcttccag aggttgtcgc
     2941 ccttgaccac ggtgtacatc cgcgattccg gcgcggactg cttggcgatc agggccgaat
     3001 cgacctgcgc gacgccgatg gtgttgccca gcgccaggat gatcttctcg gcgtcggcct
     3061 gcgacatggc gttgcccgag accttcacct tgtcgccgtc gacggagatc gaaagcccgt
     3121 cggccttcag gccgtgcttt tccacttcct tcttgagctc gtcagcggtg gccgccttgg
     3181 cctcgctcgc gccgaacagt ttcgcgccaa cgctcttggc gaatgaaaac agacccatga
     3241 gttcctccag ttcggggctt gccccggtgc cctctcgcgt actccgcgat gcgacgccac
     3301 gcaagtgcgc gcgcggatgg cgtcataggc aggcgcggtg aaccggggtt gaatgcgtgg
     3361 cggcgccggg caggcgatca ggaggtcgcc gtgcgttcgc ccttgcccgg cgcgagccgt
     3421 gccagcatcg ccaccagcgc gcggtcctgg aaggtgatgg ccttctcgcg attggagagc
     3481 caggccgccg cgtccgccac ggtttccgcg tcggcgatct tggcctccgc caccacatgg
     3541 tcgcggtcga tctcgccgcg tcggcgtggc gcgtgggcgg gcagcatcgc catatggcga
     3601 tcgcgcagcc gttcatccct gtatagcgca aggacgccgt tgacctcgtc gaagtcacca
     3661 taatgcgcct gaagcgcgtt ggcacgggtc gcgatggcag gggggctgcg ttcttccggc
     3721 gtccacagga tgccgaggcg acgcaacgcc aggccgaggc cgacaaggcc ggtcgcccag
     3781 gagatcagga tcgccagcac caggccgacc agcgtcggcg acatccacag gaacagcgag
     3841 ggggagatca gccaggtgat gatgccggcc aggacgccga aggtcacatg catcctgtgt
     3901 cgccgcacga tgtcgcggag cgggatggac ccgtcgtcgc gccgctgcgg cttccagccg
     3961 gtatcgccgc cgaacaggat ctggaaaacc gctcctgact ggatgatcat catgatcggc
     4021 gcgatcaatg ccgacatgat gatttccagc agcgcggaga ggatcagcag gattcccccg
     4081 ccgtgggcgc gccggtcttc cgggcgcgtg agtgccagga tcacgccgaa cagcttgggc
     4141 gccagcagga tgcccatggt gaccacgaac aggttgagcg cgcgctccgc gtcgaaccga
     4201 ggccaggccg ggaacagcga gaattcggag gtgaagtatt ccggacggat gaagaacgcc
     4261 tgcaacacca gcacgatgcc gacggcaagc tgcatcagcc agaacggcga ggcgagatag
     4321 gacacgatgc cggtcagaaa atggtgccgg gtcgcgggta cgaacccacg cgcgaacagg
     4381 atgcgcgagt gttgcaggtt gccctggcac cagcgccggt ctcgcgctgc caggtcgatc
     4441 agcgagggcg ggctttcctc gtacgagccg acaagccccg gcaccatggt gaccttccag
     4501 ccggcgcggc ggatcagcgc cgcctcgacg aagtcgtggc tgaggatatg gccgccgaac
     4561 ggcggcttgc ccttcaactc gggcattgcg caatgatcgg cgaaggcccg cgtgcggatg
     4621 atcgcgttgt gcccccaata gttgccgtca ctgcccatcc acaggcgtag cccttccgcg
     4681 atcaccgggc cgtagacccg ggccgcgaac tgttgcagcc gggcgaacag cgtgttgcgg
     4741 ttgatgatca gcggcagcgt ctggatgatg ccggaatcgg gatcggcctc catcaggccg
     4801 gccagcgcga cgatggtgtc gccgtcgacg aggctgtcgg cgtccagcac cagcatgtgt
     4861 tcgtaggcgc caccccagcg gctgacgaaa tcggcgatgt tgccggcctt ccgcccggta
     4921 ttcttgatcc ggtgccggta gtagagcgcg atctccgccg ggaacttctc gcgcagcgcg
     4981 aaataggccc gctcttcgcc gatccaggcg tccggatcgg ttgtgtcgga caggaagaaa
     5041 aagtcgaaat gctggcccag cccggtcgcg gccacgcctt ccgccatcgt ctgcatggcg
     5101 gcgaagacgc ggctcggggc ctcgttgtag atcggcatca ccacggcggt gcgggtcgag
     5161 agcgtccggg gaaccgtcag gcgtcccggc cggcgcagca gcaacgacag gaagcccgtg
     5221 atcgcgccgg tgaaggccag cgcgatccac acgaagttga ttacgaacag cgccagcagc
     5281 acccactgca ggaaggtcac gccgctgccc gcgtcgacga cccgtcgcat ctgccacgcg
     5341 ccgtagccgg tgagcacgag accgccgccg aacacgaaca ggcgaaccac ccacggcggc
     5401 gcgaaggcgt cccgcgcgac gggggcgcga cgttcggcct cggtccagcg ccggaaatcc
     5461 tggaccggca tggccagggg cacttcgttc ggcatggccg ggtgcgtacg ctggtcgtgc
     5521 cgacgcccct cctgcatggg cgtcacgagg tccatcgata gagccacgtt tcagtcagcg
     5581 gcgtgtcgcc gttcttcagg ttcagacgca actcgatcag gctctcgttt ccggtatcga
     5641 ggtcgaagac gacgcgcgtc accttcccgc ccgcgccggg gatgaggcgg acattgctga
     5701 tgccgttgtt cgaggcccag agattgggca cgatctccgg cggcttcgct gcggccagct
     5761 tgtcgccggc gaaatccacg acgaagcgac gacggcg
//
LOCUS       DAOMXN010000169         5785 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_7354_length_5785_cov_9.022862, whole genome shotgun sequence.
ACCESSION   DAOMXN010000169 DAOMXN010000000
VERSION     DAOMXN010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 5785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5785
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7354_length_5785_cov_9.022862"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="PK812_10925"
     CDS             complement(<1..579)
                     /locus_tag="PK812_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009489261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HRJ70110.1"
                     /translation="MSKDRVYLFDTTLRDGAQTTGVDFSLADKRQVAAMLDRLGVDYI
                     EGGYPGANPLDTKFFADPPKTRARFTAFGMTKRAGVSASNDPGLAGLLAAKADAICYV
                     AKSWDYHVHVALGCTLEENLESIADSVKAAAERGFEPMVDCEHFFDGYKANPDYALAC
                     ARTAFEAGARWVVLCDTNGGTLPHEVAEIVAAA"
     gene            complement(594..1094)
                     /locus_tag="PK812_10930"
     CDS             complement(594..1094)
                     /locus_tag="PK812_10930"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRJ70111.1"
                     /translation="MTRPVLRPFLPDDAEACDDLRVAAIFELAEDDYSEDQRAAWAAM
                     SDAAALAARLAAGLTLVVASEDEGIVGFGTLKDNSHIEFLFTRPDFARRGVATLVMDA
                     LEKLAAARGAKKLTTDASDTGQPFFAARGYVAVRRNTVSVDGEWLANTTMEKTLVGGL
                     APQGRA"
     gene            complement(1091..1294)
                     /locus_tag="PK812_10935"
     CDS             complement(1091..1294)
                     /locus_tag="PK812_10935"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HRJ70112.1"
                     /translation="MRIDGIQHGFAPNAASDRKRDAAFAMDGFLTLRFTKDDVHRNME
                     DVIETVFLRGQERLPACSPGDVP"
     gene            complement(1291..2706)
                     /gene="cysS"
                     /locus_tag="PK812_10940"
     CDS             complement(1291..2706)
                     /gene="cysS"
                     /locus_tag="PK812_10940"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRJ70113.1"
                     /translation="MTGQARLKLYNTLARAKQDFVPLDPANVRMYACGPTVYDYAHIG
                     NARPAIVFDVLFRLLRHVYGDGHVTYVRNITDVDDKINQRAFERWTRKRSLNEEIREL
                     TRVTADQYFADVAALGTLSPTHQPRATDYVCDGAKPLDMVRLIEGLVARGHAYAAEGH
                     VLFHVPSMPAYGRLARRSQDDMVAGARVEVAPYKRDPMDFVLWKPSNPETEPGWDSPW
                     GFGRPGWHIECSAMSGALLGAVFDIHAGGLDLIFPHHENEIAQSCCFSGKDVMANYWL
                     HNGFLQVEGQKMSKSLGNFITIHDLLSTDTFGGRSWPGEVLRFAMLKTHYRQPIDWTL
                     KGLEEAEGTLRRLYEKVSGIQPGSPSDDVIAALADDLNTPSAIAELHRIADTTRLAAS
                     LRLMGFSGDPARIGVVAATVDNALRAQVEALIAARNAARAAKNFAESDRLRDELATLG
                     VALKDGKDASGNPVTTWEVKR"
     gene            3025..3330
                     /locus_tag="PK812_10945"
     CDS             3025..3330
                     /locus_tag="PK812_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70114.1"
                     /translation="MLRFAALSIAVAISAYGVVTAQSASSQPKQKEFVIRNDDGYGTS
                     DCLSKASACGKIVADAWCEAKGFGKAVAFRPVEPTEITNSTGRSSPVADAFLISCSE"
     gene            complement(3355..3852)
                     /locus_tag="PK812_10950"
     CDS             complement(3355..3852)
                     /locus_tag="PK812_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009489255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HRJ70115.1"
                     /translation="MRRAALLAASLVVATAAAAQEPDLIFKKSTVFKLLTPDDKLATY
                     AIDDPEVEGVACHFTVPETGGLTGALGLAEHLSDISLACRQIGPVKFKSKMAQGDVVY
                     RQSRSLFFKRMQVVRGCDAKRNVLVYLVYSDKLIDGSPKNSTSTVPIMPWGSGEPPKC
                     AEFVK"
     gene            complement(3942..4334)
                     /locus_tag="PK812_10955"
     CDS             complement(3942..4334)
                     /locus_tag="PK812_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012706955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRJ70116.1"
                     /translation="MFSHVTVGTNDGAKAKAFYGKVLGALGITLAHDLEEYGWYGFGR
                     PGDAQAGFWVGKPANGGPATFANGGTIAFNAAKRSEVDAFHKAAMEQGGTDEGGPGLR
                     PHYHPNYYGAYVRDPDGNKLCCVCHRPE"
     gene            4469..5554
                     /gene="dctP"
                     /locus_tag="PK812_10960"
     CDS             4469..5554
                     /gene="dctP"
                     /locus_tag="PK812_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HRJ70117.1"
                     /translation="MKRRQFLAAGAGGLAATAIAKPAIAQAMPEVRWRLTSSFPKNLD
                     TIYGTAETFARFMREATDGKFQIQTFSGGEIVPPLQALDAVTNGSVELAHTPTYFYFG
                     KDPTLAFGTGVPFGLNARLQHAWWHFAGGGDIVNEALRKFNAIGLPCGNSGTQMGGWF
                     RKEINSIDDLKGLKFRIGGTGGIIMQKLGVVPQQIAAGDVYPALERGTLDAAEFVGPY
                     DDEKLGFAKVAPYYYYPGWWEGGAMLHLCINLEKWNSLTPTYQAILRQAAEAANNWML
                     AKYDAVNAPALLRLIRAGVQVKPFPVATIDAAYKATQEIFADIGSKNPLFKKANDSMV
                     AFRGEQYPWWAVGEYSYDGYMIRAVRS"
BASE COUNT          974 a         1910 c         1885 g         1016 t
ORIGIN      
        1 cgccgccgcg acgatttccg ccacctcgtg cggcagcgtg ccgccgttgg tgtcgcacag
       61 caccacccag cgcgcgccgg cctcgaacgc cgtgcgggcg caggcgagcg catagtccgg
      121 attggccttg tagccgtcga agaaatgctc gcaatcgacc atcggctcga agccgcgctc
      181 ggcagcggcc ttcacgctgt cggcgatcga ttcgaggttt tcctccagcg tgcagccaag
      241 cgcgacgtgc acgtgatagt cccacgattt cgcgacgtag cagatcgcgt ccgccttcgc
      301 ggccagcagc ccggccagac cggggtcgtt ggacgccgag acgccggcgc gcttggtcat
      361 gccgaaggcg gtgaagcgcg ccctggtctt cggcggatcg gcgaagaact tcgtgtccag
      421 cgggttggcg ccgggatagc cgccctcgat gtagtcgacg ccaagccggt ccagcatggc
      481 ggcgacctgc cgcttgtcgg cgagcgagaa atcgacgccg gtggtctgcg cgccgtcacg
      541 cagcgtggtg tcgaagagat agacgcggtc cttgctcata gcagccggct tcctcatgcc
      601 ctgccctgcg gcgcgaggcc gccgacaagc gttttttcca tcgtggtgtt ggcgagccat
      661 tcgccatcga ccgatacggt attgcgccgc accgccacat agccacgcgc cgcgaagaag
      721 ggctgccctg tgtcgctggc gtcggtcgtc agttttttcg cgccgcgcgc ggcggcgagc
      781 ttttccagag cgtccatcac cagcgtcgcg acgccgcgcc tcgcgaagtc gggccgcgtg
      841 aacaggaatt ctatgtggga attgtccttc agcgtgccga acccgacgat cccctcgtcc
      901 tcgctggcga cgacaagcgt caaccccgcc gcaaggcgcg cggccagcgc cgcggcgtcg
      961 ctcatggcgg cccaggcggc gcgctggtcc tcggagtaat cgtcttcggc cagttcgaaa
     1021 atggcggcga cgcgcagatc gtcgcacgcc tcggcgtcat ccggcaggaa ggggcgaagc
     1081 acggggcggg tcatgggacg tccccggggg agcatgccgg caagcgctcc tgcccgcgca
     1141 ggaagaccgt ttcgatcacg tcttccatat tccggtggac gtcgtctttg gtgaaccgca
     1201 gggtgagaaa gccatccatg gcgaaagccg cgtcccgctt ccggtcgctc gccgcattcg
     1261 gcgcaaagcc atgctgaatg ccgtcgatcc tcaccgcttc acctcccagg tggtgacagg
     1321 attgccgctc gcgtccttgc cgtccttcag cgcgacgccg agggtggcaa gttcgtcgcg
     1381 caggcggtcg gattcggcga aattcttcgc tgcgcgggcc gcattgcggg cggcaatgag
     1441 ggcttcgacc tgcgccctga gggcattgtc gactgtcgcc gcgaccacgc cgatccgtgc
     1501 cgggtcaccg gaaaagccca tcaggcgcag cgaagccgcg agccgggtgg tgtccgcgat
     1561 ccggtgcagt tcggcgatgg cggacggcgt attgagatcg tctgccagcg cggcgatgac
     1621 gtcatccgac ggtgagccgg gctggatgcc cgaaaccttc tcgtagagcc gtcgcagcgt
     1681 gccttccgcc tcctccaggc ccttcagcgt ccaatcgatg ggctggcggt agtgcgtctt
     1741 cagcatggcg aagcgcagca cctcgcccgg ccaggagcgg ccgccgaacg tgtcggtcga
     1801 aagcaggtcg tggatggtga tgaagttgcc gagcgatttc gacatcttct ggccttccac
     1861 ctgcaggaag ccgttgtgga gccagtaatt ggccatcaca tccttgccgg agaagcagca
     1921 ggactgggcg atctcgttct cgtggtgcgg gaagatcagg tcgagcccgc cggcgtggat
     1981 gtcgaacacc gcgccgagca gggcgccgga catggccgag cactcgatat gccagcccgg
     2041 ccgtccaaac ccccaggggc tgtcccaacc cggctctgtc tcggggttgg agggcttcca
     2101 cagcacgaaa tccatcggat cgcgcttgta gggcgcgacc tcgacgcggg cgccggccac
     2161 catgtcgtcc tgcgagcggc gggccagacg accataggcg ggcatcgacg gcacatggaa
     2221 cagcacgtgg ccttccgccg cataggcatg gccgcgcgcc accagtccct cgatcagccg
     2281 caccatgtcg agcggcttgg ccccgtcgca gacgtagtcc gtggcgcgcg gctgatgggt
     2341 gggggaaagc gtgccgagcg ccgcgacatc ggcgaaatac tggtccgccg tgacacgcgt
     2401 cagctcgcgg atctcctcgt tgaggctgcg cttcctggtc caccgctcga aggcgcgctg
     2461 attgatcttg tcgtcgacgt ccgtgatgtt gcggacatag gtgacatggc cgtcgccgta
     2521 gacatggcgc agcagccgga acagcacgtc gaagacgatg gccgggcgcg cgttgccgat
     2581 atgggcgtag tcgtagaccg tcgggccgca ggcatacatg cgcacattgg ccggatcgag
     2641 cggaacgaag tcctgcttcg cgcgcgccag cgtgttgtac agtttcagtc tcgcctgccc
     2701 tgtcatggcg tcctcacgtc gatgtgaagc tggcctgccg gccggcgggt ctgttttcct
     2761 ctggtgtttt cggagaacga gacagcgctg gccagcagga agctagccgc aaataatccc
     2821 ggaaatgatc gcaaccgcgc gcgtcatgag cgtttcatgc tgtttttgca ggctgtcgtc
     2881 aagcgggggg cgccacagac ttatgttgca acgcagcaac gctcggtccg gtttcgacat
     2941 gtgcgttctt gcacatcgcg gcgttagcct tttcttcact gttccccggc agcttcccac
     3001 tgaatcgcct tctggagaat cccgatgctt cgcttcgccg ccctttccat cgccgtcgcc
     3061 atctcggcct atggcgtggt gaccgcgcag tcggcgtcga gccagccgaa acagaaggaa
     3121 ttcgtcatcc gcaacgatga cggctacggc acgtcggact gcctctccaa ggccagcgcc
     3181 tgcggcaaga tcgtcgccga tgcctggtgc gaggcgaaag gcttcggcaa ggcggtggcg
     3241 ttccgtccgg tcgagccgac cgagatcacc aactcgaccg gccgctcaag ccccgtcgcg
     3301 gacgcgttcc tgatctcctg ctcggaatga ccggcgcgcg gcgccagccc gccgctactt
     3361 cacgaattcg gcgcatttcg gcggctcgcc gctgccccag ggcatgatcg gcaccgtcga
     3421 ggtggaattc ttcgggctgc cgtcgatcag cttgtcggaa tagacgagat agaccagcac
     3481 gttgcgcttg gcgtcgcagc cgcgcaccac ctgcatgcgc ttgaagaaaa gcgaacgaga
     3541 ctggcgatac accacatcgc cctgcgccat cttcgacttg aacttcaccg ggccgatctg
     3601 gcgacaggcc agcgagatgt cggagaggtg ttcggccagg cccagcgccc cggtcaggcc
     3661 accggtttcc ggcacggtga aatggcaggc gacgccctcc acttccggat cgtcgatggc
     3721 gtaggtcgcc agcttgtcgt ccggcgtcag cagcttgaac accgtcgatt tcttgaagat
     3781 caggtccggc tcctgcgctg cggccgccgt ggcgacgacg agcgaggcgg cgagaagggc
     3841 ggcgcggcgc atgggacact cctttgcgaa acctgagcca gatatggccc gcgccgcgcc
     3901 ggtttgaaag aggctgcctc ccggtccgaa ggcccgacgc cctattccgg cctgtgacag
     3961 acgcagcaga gcttgttgcc gtccgggtcg cgcacatagg cgccgtagta gttcggatga
     4021 tagtgcgggc gcagtcccgg accaccctcg tcggtgccgc cctgctccat cgcggccttg
     4081 tggaaagcat ccacctcgga ccgcttcgcc gcattgaagg caatggtgcc gccgttggcg
     4141 aaggtggccg gcccgccatt cgccggcttg ccgacccaga agccggcctg cgcgtcgccg
     4201 ggacgaccga aaccgtacca gccgtattcc tcaagatcgt gggcgagcgt gatgccgagg
     4261 gcgcccagca cctttccata aaacgccttc gccttggcgc cgtcgttggt gccgaccgtg
     4321 acatggctga acattctgta tcctcctgta tagaacataa caggaacatg ttgcccgccg
     4381 tcctgtcaat ccgtgtcagg agcgattccg gttgatcacc aacggcggat gttgaaaagt
     4441 ccgcagcttc ttgatcaggg aggcattgat gaagcgcaga cagttcctcg ccgccggagc
     4501 aggcggacta gcggccactg ccatcgcgaa gccggcgatc gcgcaggcga tgccggaggt
     4561 ccgctggcgg ctgacatcga gctttccgaa gaacctcgac acgatctacg gcacggccga
     4621 aaccttcgcc cgcttcatgc gcgaggcgac cgacggcaag ttccagatcc agaccttctc
     4681 cggcggcgag atcgtcccgc cgctgcaggc gctcgatgcg gtgaccaacg gctcggtcga
     4741 gctggcgcac accccgacct atttctactt cggcaaggac ccgacgctgg ccttcggcac
     4801 cggcgtgccg ttcggcctca atgcccgcct ccagcacgcc tggtggcatt tcgccggcgg
     4861 cggcgacatc gtcaacgagg ccttgaggaa attcaacgcc atcggcctgc cctgcggcaa
     4921 ctccggcacg cagatgggcg gctggttccg caaggagatc aacagcatcg acgatctcaa
     4981 gggcctgaag ttccgcatcg gcggcacggg cggcatcatc atgcagaagc tcggcgtcgt
     5041 gccgcagcag atcgccgccg gcgacgtcta cccggcgctg gaacgcggca cgctcgacgc
     5101 ggcagagttc gtcggcccct atgacgacga gaagctcggc ttcgccaagg tcgcgcccta
     5161 ctactactat cccggctggt gggagggcgg cgctatgctg cacctgtgca tcaacctgga
     5221 aaagtggaat tccctcacac cgacctatca ggcgatcctg cggcaggcgg ccgaggccgc
     5281 caacaactgg atgctcgcca agtacgacgc ggtgaatgcc ccggcgctgc tgcggctcat
     5341 ccgcgccggc gtacaggtca agcctttccc ggtcgcgacc atcgatgccg cctacaaggc
     5401 gacgcaggag atcttcgcgg acatcggctc gaagaacccg ctgttcaaga aggcaaacga
     5461 ttcgatggtt gctttccgtg gcgagcaata cccctggtgg gcggtcggcg agtattcgta
     5521 cgacggctac atgatccgcg cggtccggag ctgaatggcc ggcggcttcc ggccgcctgc
     5581 cccttccaat tgcggcatta atggtgcagt aaccatcccg gaacacgatg gggcgatgtg
     5641 aaggcctcag gtggaggacc ggttgctgtt ggccccgaca acccagatgc tgcgccgcgc
     5701 actgtcctgc gcggcgctgg cgtttgcggc gctggcgggc atgaatgtcg cggccggcgc
     5761 gcagtccgcc gtctgtcgcc agctt
//
LOCUS       DAOMXN010000170         5766 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_7385_length_5766_cov_11.948871, whole genome shotgun sequence.
ACCESSION   DAOMXN010000170 DAOMXN010000000
VERSION     DAOMXN010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 5766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5766
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7385_length_5766_cov_11.948871"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="PK812_10965"
     CDS             complement(<1..467)
                     /locus_tag="PK812_10965"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HRJ70118.1"
                     /translation="MLGARFAGDGRIELQAQSLPEPAAGEVRVRVAACCLCGSELRQW
                     RQGWPVTPGHEISGVVDQPGHARHGQRVAVYIPVFCDTCEECGAGQTHLCRNATDLIG
                     WQRAGGYAEMLNVPERCLLPLPGDVPLDLAPLLLDTIGTAAHGTHNTKVKRQQ"
     gene            complement(471..1757)
                     /locus_tag="PK812_10970"
     CDS             complement(471..1757)
                     /locus_tag="PK812_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009755761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRJ70119.1"
                     /translation="MAITLSILVAVVLVVLGMPFWVSLGLATAALLWSTGVMPLQLIG
                     EALFEGVDSFALIAIPLFVLTGDVMVRSGLAYRLLNFAEASFGSLRSGFGTATVMGCG
                     LFACISGSDAADAAAIGRITMNKLVEKGYPKPYACALVAAGACTGILIPPSIAYIIIG
                     LVLGISSATLFQAAFVPGVMILAAVMITNAIVNRARGYEISTGKFSFPVWLAALNDAK
                     WALLIPFIILGGIYSGIFTPTESAAVAAFVALLIGMVQKRITLADFPAMLGASARVCG
                     VVLPIIAVALLFAQALTVVDVPQKFVATMMSFADAIPFVDQKTGVILTMLVIWIIAGC
                     FMETGPNIVVLGPLMLPLAKAIGMDPIHFCVFMITTLGLGFITPPFGLNLFVMSGLTR
                     TPVTAISKQAVPFVVAMIIVSAIIGFVPAISLFTLR"
     gene            complement(1760..2278)
                     /locus_tag="PK812_10975"
     CDS             complement(1760..2278)
                     /locus_tag="PK812_10975"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRJ70120.1"
                     /translation="MKRLIDDLDENVERYLMLVCYVFCCAVIIHDVGRRFLMNYSAAW
                     SQETAQYAFIYLGWIGAAFAVKERAHIRFDIVQGMLPERLHVWIYVAGEVCTILFAVI
                     ALRYSLHTIEQLWQFGGATPVLRVSKIWAEAAVPIGFSLIILRSLQRLKHDFAALRAN
                     EPVFKGKALFED"
     gene            complement(2275..2490)
                     /locus_tag="PK812_10980"
     CDS             complement(2275..2490)
                     /locus_tag="PK812_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70121.1"
                     /translation="MLRAKPWLGLSAGLVVLYLVYLLWSKFARALGAPPVRLSETGEF
                     LFFFLAIVAFTVQVIVDERRTKGSAGR"
     gene            complement(2548..3687)
                     /locus_tag="PK812_10985"
     CDS             complement(2548..3687)
                     /locus_tag="PK812_10985"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRJ70122.1"
                     /translation="MKHYVTDPMERRQFLKVAARYGFGVAVASALGGHLFDSQAVAQT
                     ASDEAAKEAAAKVKMNFATEYKIEDWVKYPVMQAKFKENVEQASKNEIFVKLFPAGAL
                     GIGAALAQKIQSGTVHGGAVSLSNFSPFTPIVDMINIPYWCGENQKFANLVTSAAWND
                     EITPKVMEKGYKPMFYYTVDPRTIASRKGVKPIKTPADMSGIKMRVPPSAMLQQIYKL
                     AGANPTVVAWGETAAAIKQGVADALDPAITALATFGFHDILGNVTFVKTVPDAQMFAA
                     NAGWYRSLNDAQRKAFDDATAKTQIETFAQIESARKESMRIMEANGCAFHRLSDAEYK
                     QWVDACGEQKKEWDEFKVKLAGSLDNFEKYKKAANTKGPITVTDM"
     gene            3874..4896
                     /locus_tag="PK812_10990"
     CDS             3874..4896
                     /locus_tag="PK812_10990"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRJ70123.1"
                     /translation="MVPSSRPTLDDVARAASVSAATVSRAISRPDMVKSGTLARIGEA
                     IRSLGYIPTGPARALASGRSGTIAAVVPTLDNAIFSRALQAMQGVLSAEGYQLLVAPH
                     NYSMAGEAESVRLLMSRGIDGLMLVGAERAPELVSLLATTGIPVLCTWIARAGEAGVT
                     VDNERAGWIAAEHLFQLGHRRIGMVTGLQAFNDRQKARLRGVRRFLAQAGQALPDWLV
                     SQQPTTLAGGRVGCSTLLALSQPPTALIGGIDLIALGCVIESQARGLTVPGDISVVGI
                     DDLDISAHMAPALTTVHIPTAVIGEEAGRMLIAMIRDRDSGGLIDLPVELVVRQTSGP
                     PRNALR"
     gene            5119..5577
                     /locus_tag="PK812_10995"
     CDS             5119..5577
                     /locus_tag="PK812_10995"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRJ70124.1"
                     /translation="MLGAGQAGARVAEPLPALYPGTILVSTGRRRLYFALPNGQALSY
                     PVAVGKPGKQWFGQRMIDGKHVRPAWSPPDDVKRDNPHLPAVIPGGAPNNPMGERALT
                     IAPGQYAIHGTNRPESIGAWASYGCIRMHNRDIIDLFDRVTVGAPIIVVR"
     gene            complement(5561..>5766)
                     /locus_tag="PK812_11000"
     CDS             complement(5561..>5766)
                     /locus_tag="PK812_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70125.1"
                     /translation="NALRALLAAGGISLVEVGRDKYGGRVLAGLLTREGRDVAGLMLA
                     SGQARPYAGGRRAPWCASLSARR"
BASE COUNT         1044 a         1901 c         1868 g          953 t
ORIGIN      
        1 tactgctggc gctttacttt ggtattgtgc gtcccatgcg ccgccgtgcc gatggtgtcg
       61 agcagcagcg gcgcaagatc gagaggcacg tcgcccggca gcggcagcag gcatcgttcc
      121 ggcacattca gcatctcggc atagccgccg gcgcgctgcc agccgatgag atcggttgcg
      181 ttgcggcaca gatgcgtctg acccgcgcca cactcctcgc aggtatcgca aaagacagga
      241 atgtacaccg cgacgcgctg cccgtgccgg gcatgcccgg gctgatcgac gacgccggag
      301 atctcgtgcc caggcgtcac cggccagccc tggcgccact gcctcagttc ggacccacac
      361 aggcagcagg ccgccacgcg gacgcgcacc tcgccggccg ccggttcggg cagcgactgt
      421 gcctgaagct cgatacggcc atcgccggcg aaacgagcgc ccagcatggc tcagcgcagc
      481 gtgaacagcg agatcgccgg cacgaagccg atgatggccg agacgatgat catggcgaca
      541 acgaagggca cggcctgctt cgatatggcg gtgaccggcg tgcgcgtcag tccgctcatg
      601 acgaacaggt tgaggccgaa cggcggcgtg atgaagccga ggccgagcgt ggtgatcatg
      661 aagacgcaga aatggatcgg gtccatgccg atcgccttgg ccagcggcag catcagcggc
      721 ccgagcacca cgatgttcgg cccggtctcc atgaaacatc ccgcaatgat ccagatcacc
      781 agcatggtca ggatcacccc ggtcttctgg tcgacgaagg ggatggcgtc ggcgaaggac
      841 atcatggtcg ccacgaattt ctgcggcacg tcgacgaccg tcagcgcctg cgcgaacagc
      901 agggccactg cgatgatcgg cagcacgacg ccgcaaacgc gggccgacgc gccgagcatg
      961 gcgggaaagt cggcaagcgt gatccgcttt tgaaccatgc cgatgagcag cgccacgaag
     1021 gcggccaccg ccgcggattc cgtcggcgtg aagatgccgg aataaatgcc gccaagaatg
     1081 atgaagggaa tgagcagcgc ccatttcgcg tcgttcaggg ccgccagcca gaccgggaaa
     1141 gagaacttgc ctgtcgagat ctcgtagccg cgcgcgcggt tgacgatggc attggtgatc
     1201 atcaccgcag cgaggatcat gacgcctggc acaaaggcgg cctggaacag cgtcgccgag
     1261 gaaatgccga gcaccaggcc gatgatgatg taggcgatcg agggggggat caggatgccg
     1321 gtgcaggcgc cggcggcgac caaagcgcag gcgtagggct tgggatagcc cttttccacc
     1381 agcttgttca tggtgatgcg gccgatggcg gccgcatccg ccgcatccga cccggagatg
     1441 caggcgaaca ggccgcagcc catgaccgtg gccgtgccaa atccggagcg cagcgaaccg
     1501 aagctggctt ccgcgaaatt gagcaggcga taggcaaggc cggagcgcac catcacgtcg
     1561 ccggtcagca cgaacagggg gatggcgatc agcgcaaaac tgtcgacgcc ttcgaacagc
     1621 gcctcgccga tcagttgcag cggcatgacg ccggtcgacc agagcagcgc ggccgtggcc
     1681 agtcccaggg acacccagaa cggcatcccc agcacgacga gcacgacggc aacgagaatg
     1741 gaaagggtga tcgccatggt cagtcctcga acagcgcctt gcccttgaag accggttcat
     1801 tggcgcgcaa cgccgcgaaa tcgtgcttca ggcgctgcag cgaacgcaga atgatgagcg
     1861 agaagccaat cggcacagcc gcttccgccc agatcttgga cacgcgcaac accggggtcg
     1921 cgccgccgaa ctgccagagc tgctcgatcg tatgcagcga ataccgcagc gcgatcacgg
     1981 caaagaggat cgtgcagact tcgcccgcga catagatcca gacatgcagg cgctccggca
     2041 gcatgccctg cacgatgtcg aagcggatat gggcccgttc cttcacggcg aaggcagcgc
     2101 cgatccagcc gaggtagatg aaggcgtatt gcgccgtctc ctgactccag gcggcggaat
     2161 agttcatcag gaaccgccgc ccgacatcat ggatgatgac ggcgcagcag aagacgtagc
     2221 agacgagcat cagatagcgc tcgacgttct cgtcaaggtc gtcgatgaga cgcttcatcg
     2281 gccggcgctc cccttggtgc gccgctcgtc aacgatcacc tgcacagtga aggcgacgat
     2341 cgccaagaag aagaacagga actcgcccgt ctcgctaaga cggacaggcg gcgcgccgag
     2401 cgcgcgcgcg aacttcgacc agagcagata gacgaggtaa agcacgacca ggcctgcgga
     2461 caggccaagc cacggtttgg cgcgaagcat ggtcggcctc cgatcggtgg cgggagcggg
     2521 gcggcggacg ggccgccccg aagtcgacta catgtcggtg acggtgatcg gacccttggt
     2581 gttggcggcc ttcttgtact tctcgaaatt gtcgagcgag ccggcgagct tgaccttaaa
     2641 ctcgtcccat tccttcttct gttcgccgca ggcgtcgacc cattgcttgt actcggcatc
     2701 cgacaggcga tggaaggcgc agccgttcgc ctccatgatg cgcatgctct ccttccgcgc
     2761 gctttctatc tgcgcgaagg tctcgatctg ggtcttggcc gtcgcgtcat cgaaggcctt
     2821 gcgttgcgcg tcattcaggc tgcgatacca gccggcgttg gccgcgaaca tctgtgcgtc
     2881 cggcaccgtc ttgacgaagg tgacgttgcc caggatgtca tggaagccaa aggtcgccag
     2941 cgcggtgatc gccgggtcca acgcgtcggc gacgccctgc ttgatcgctg cggcggtctc
     3001 gccccaggcg accacggtcg ggttggcgcc agcgagcttg tagatctgct gaagcatggc
     3061 ggacggcggc acgcgcatct tgatgccgga catgtcggcc ggcgtcttga tcggcttcac
     3121 gcccttgcgg ctggcgatcg tgcgtggatc gacggtgtag tagaacatgg gcttgtagcc
     3181 cttttccatc accttcggcg tgatctcgtc gttccaggcg gcgctggtca cgagattcgc
     3241 gaacttctgg ttctcgccgc accagtacgg gatgttgatc atgtccacga tcggcgtgaa
     3301 cggcgagaag ttcgacagcg aaacggcacc gccatgcacc gtgccggact ggatcttctg
     3361 cgccaaggcc gcgccgatgc cgagcgctcc ggccgggaac agcttgacga aaatctcgtt
     3421 cttggaagcc tgttcgacgt tctccttgaa cttggcctgc atcaccgggt acttcaccca
     3481 gtcctcgatc ttgtactcgg tggcgaagtt catcttcacc ttggcggccg cctccttcgc
     3541 cgcctcgtcg ctggcggtct gggccacggc ctggctgtcg aacagatgcc cgccgagcgc
     3601 gctggcaacg gccacgccga aaccgtagcg cgccgcaact ttcaggaact gccgccgctc
     3661 catgggatcg gtcacatagt gcttcatcgt tttctccctc gccgcctgtc gtaccgtcgg
     3721 gaaatcccgc caggtcgcag tcattatgaa agcgcttgca gtttgtttca gaaacgcggc
     3781 aacgcgtcaa acgcaagatt cgctgcatcg caacacgacc gtcgaggcca aaagatttct
     3841 tgcaaaatct tgcataccgg gcatcgtggt ggaatggttc cgtcctcccg tcccacgctc
     3901 gacgatgtgg cgcgcgccgc gtcggtttcc gccgcgacgg tttcccgcgc gatttcgagg
     3961 ccggacatgg tgaagtccgg gacgcttgcg cgcatcggcg aagcgatccg ctcccttggc
     4021 tatatcccca ccggtccggc ccgtgccctg gcgtccgggc gcagcggcac catcgccgcc
     4081 gtcgttccga ccctcgacaa cgccatcttc tcgcgggccc tgcaggccat gcagggcgtg
     4141 ctgtcggcgg aaggctacca actgctggtg gcaccgcata actacagcat ggcgggcgag
     4201 gcggagtcgg tgcgcctgct gatgtctcgc ggcatcgacg ggctgatgct ggtcggcgcc
     4261 gagcgggctc cggaactggt atcgttgctc gcgacgacag gcataccggt cctctgcacc
     4321 tggattgccc gcgccggaga ggccggcgtg acggtcgaca acgagcgcgc cggctggatc
     4381 gcggccgagc atctgttcca actcgggcat cgccgcatcg gcatggtcac cggcttgcag
     4441 gctttcaacg atcgccagaa ggcccgcttg cgcggggtgc ggcgcttcct tgcgcaagcg
     4501 ggtcaggccc tgccggattg gctggtgtcg cagcagccga ccacgttggc cggcgggcgc
     4561 gtcggctgtt cgacgctgct ggccctgtcg caaccgccca cggcgctgat cggcggcatc
     4621 gacctcatcg cgctcggctg cgttatcgag agccaggcgc ggggcctgac ggtacccggc
     4681 gacatctcgg tcgtcggcat cgatgatctc gacatctcgg cgcacatggc gcccgcgctc
     4741 acgacggttc acatcccgac agcagtgatc ggcgaggagg cgggccgcat gctgatcgcg
     4801 atgatccgtg atcgtgactc cggtggtttg atcgatctcc cggtcgaact cgtggtgcgc
     4861 cagaccagcg gaccgccgcg caacgccctg cgctaacgcg ttgccggaag gccttgggcc
     4921 cgggccaggc cctggactgg ccatcgatcg cgagaccgca gcggcattca cccctgcgta
     4981 aggttaattc caggcgtttg cgtcccgtat accgctgctc aacgccctac ccgccaaatc
     5041 cgcatggacg aaccacgggg aaggtgcgtg cagattctga ccagacgcct gttcggctgt
     5101 ggcctgctgg cattacccgt gcttggcgcc gggcaggccg gggcgcgcgt cgctgagccc
     5161 ttgccggccc tctaccccgg caccatcctg gtctcgacag ggcgccgccg gctctacttc
     5221 gcccttccca acgggcaggc cctgagctac cccgtcgcgg tcggcaagcc cggcaagcaa
     5281 tggttcggcc agcgcatgat cgacggcaag cacgtgcgcc cggcctggag ccctccggac
     5341 gacgtcaagc gcgacaatcc gcatcttccc gccgtcattc ccggcggcgc gccgaacaac
     5401 cccatggggg aacgggcatt gaccatcgcg ccgggtcaat acgcgattca cggcacgaac
     5461 cggcccgaat cgatcggtgc ctgggcctcc tacggctgca tcaggatgca caaccgcgac
     5521 atcatcgatc tgttcgatcg cgtcaccgtc ggcgcgccga tcatcgtcgt gcgctgagcg
     5581 aggcacacca cggcgcccgg cggccgccgg catagggccg ggcctgtccg gaggcgagca
     5641 tcagcccggc cacgtcgcgc ccctcgcgcg tgagtaagcc ggcgagcacg cgcccgccgt
     5701 acttgtcgcg gccaacctca acgaggctga tgccgcccgc cgcaaggagc gcccgcagcg
     5761 cattgc
//
LOCUS       DAOMXN010000171         5728 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_7441_length_5728_cov_11.672131, whole genome shotgun sequence.
ACCESSION   DAOMXN010000171 DAOMXN010000000
VERSION     DAOMXN010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 5728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5728
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7441_length_5728_cov_11.672131"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1981)
                     /gene="topA"
                     /locus_tag="PK812_11005"
     CDS             complement(<1..1981)
                     /gene="topA"
                     /locus_tag="PK812_11005"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HRJ70126.1"
                     /translation="MNVVVVESPAKAKTINKYLGKDFEVLASFGHVRDLPAKDGSVEP
                     DNDFAMSWEVDGKASKRLSDIAAAVKGADRLILATDPDREGEAISWHILEVLKNKRVL
                     KGKTIQRVTFNAITKQAVQDAMAAPREIDQALVDAYLARRALDYLVGFTLSPVLWRKL
                     PGARSAGRVQSVALRLVCDRELEIEKFVRQDYWSIVATLLTPAGEAFDARLVGADGKK
                     IARLDIGNPHDAKKFKEDLETAKFSVVSVESKPTKRHPFAPFTTSTLQQEASRKLGMS
                     PQITMRVAQKLYEGADIGGETVGLITYMRTDGVDMAPEAVAQVRAYIGKNWQSKFLPA
                     APRKYTTKAKNAQEAHEAIRPTDVFRTPQQMKAHLSPDEFKLYDLIWKRTVASQMESA
                     ELERTVADIVAAVGTRRLDLRATGQVVRFDGFLGLYTEDKDDDEDEDNRRLPAMKSGD
                     PLAKKSVAATQHTTEPPPRFTEASLVKRMEELGIGRPSTYAAIIAVLKDRDYVRLEKK
                     RLVPEDTGRLLTAFLESFFSRYVEYSFTAGLEEQLDRISNSEIDWKAVLRAFWEDFNG
                     AIGGTRELRTGQILTALDDMLGPHFFPQKADGSDSRACPRCGNGRLSLKLGKTGPFIG
                     CGNYPECRYTRTLAVQGDGADSEAGTEGDGVRRLGT"
     gene            1935..3266
                     /locus_tag="PK812_11010"
     CDS             1935..3266
                     /locus_tag="PK812_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HRJ70127.1"
                     /translation="MIVFALAGDSTTTTFICAPLPMDRANYGPGREMSNGAPETEILR
                     LVASLHAMMNSPQRLPVSERPNFRFFIRRAGDPAPTFRARSASNPSYAFDSAAGRNIV
                     LCFFGSTSHETGRSALEAVARNRAIFDDDRLAFFGVSVDPADEAQGRIRDAYPGIRFF
                     HDHDGAICKLYGALPAQPEGVRNLPFRCVWVVIDPTLRILNVLNFDQTGENHEDLFAY
                     LRTLPPPGRHAGIEVPTPILVLPNVFDADFCKHLIGLYDTHGGEETGFMIERDGKTVG
                     VLDHSHKRRRDYMIEDDSIMQETQKWVQRRIIPEILKVHQFRVTRMERYMVGCYTAED
                     RGHFRPHRDNTTRGTAHRRFACSIALNDDYDGGEVGFPEYGSRTFKAPAGGAVVFSCS
                     LLHMVTPVTRGRRYVFLPFLYDDAAAEIRERNNTYLSEEIQAYDPNKKFAA"
     gene            complement(3290..4366)
                     /locus_tag="PK812_11015"
     CDS             complement(3290..4366)
                     /locus_tag="PK812_11015"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012456353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HRJ70128.1"
                     /translation="MKTYRIAAIPGDGIGTEVVAAGVEVLEAVAAREGGFAFRFDHFD
                     WGGDYYKKHGRMMPEDGRALIRNHDAILFGSAGHPDIADHITLWGLRLAICQPFDQYA
                     NVRPTRILPGITSPLRAVSGPELDWVIVRENSEGEYSGVGGRVHQGLPEEAATDVSIM
                     TRAGVTRIMRFAFKLAQSRPRKLLTVVTKSNAQRYAMVMWDEIAAEVAQEFPDVTWDK
                     AIVDAMTMRMTLKPWSIDTVVATNLHADILSDLAAALAGSLGIAPTANINPERKFPSM
                     FEPIHGSAFDIMGKGIANPVGTFWSASIMLEHLGEPAAAGRLMRAIERVTANPALHTP
                     DLGGKATTRSVTDAVIDAIQADNE"
     gene            complement(4410..5387)
                     /locus_tag="PK812_11020"
     CDS             complement(4410..5387)
                     /locus_tag="PK812_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020179198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HRJ70129.1"
                     /translation="MPFRRNILKAGALVAALASMPGLAAAQAFPTRPITLIVPAAAGG
                     PTDTVARLIAEAMGKTLGQNVVVENAGGAGGTIGMGRVAKAANDGYTVAVWHIAHATA
                     PALYKSLAYNVVDDFDHVGRITDVPMALVSRATLAANTPAELLAWMKADADKVNYGHA
                     GIGSASHLCMTMLTHELGLKLRGVAYSGTGPAMTDLLGGQFDIMCDQTTNTTNQIKDK
                     KIKGFAVTTKTPVGSLPELPTLDSSGVKGFEVSAWHAMWAPKNLPQDVKAKLSAALLA
                     ALKDPKVVERFASLGTAPVSAETAQPDALKAHLTAEVKRWGDALKGLAN"
     gene            5515..>5728
                     /locus_tag="PK812_11025"
     CDS             5515..>5728
                     /locus_tag="PK812_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011650734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRJ70130.1"
                     /translation="MDITELRCLVALGDELHFGRAAQKLDMLPASLGRYIRLLEEDLG
                     VRLVARTTRSVALTAAGVELLEEARRL"
BASE COUNT          980 a         1819 c         1800 g         1129 t
ORIGIN      
        1 cggtgcccag gcgccggacg ccatcgccct cggtcccggc ctcgctgtcc gcgccatcgc
       61 cctgcacggc cagagtccgc gtgtagcggc actcgggata gttgccgcag ccgatgaacg
      121 gtccggtctt gccgagtttc agggacagac ggccattgcc gcagcgcggg caggcgcggc
      181 tgtcgcttcc gtcggccttc tgcgggaaga aatgcgggcc gagcatgtcg tcgagcgccg
      241 tcaggatctg gccggtgcgc agttccctgg ttccgccgat ggcgccgttg aaatcctccc
      301 agaaggcgcg cagcacggcc ttccagtcga tttcactgtt cgaaatgcga tcgagctgct
      361 cttccagccc ggccgtgaag ctgtactcga cgtagcgcga aaagaagctt tcgaggaagg
      421 ccgtcagcag ccggccggta tcctccggca cgaggcgctt cttctccagc cggacatagt
      481 cgcgatcctt gagcacggcg atgatcgcgg cataggtcga gggccggccg ataccgagtt
      541 cttccatgcg cttgaccagg ctcgcctcgg tgaaacgcgg cggtggctcg gtcgtgtgct
      601 gggtcgctgc gacggacttc ttcgccagcg gatcgccgga tttcatcgcc gggaggcggc
      661 ggttgtcctc gtcctcgtcg tcgtccttgt cctccgtgta gaggccgagg aagccgtcga
      721 agcgaacgac ttgccccgtc gcccgcaggt cgagccgccg cgtgccgacc gccgcgacga
      781 tatcagccac cgtgcgctcc agttccgccg attccatctg gctcgccacc gtgcgcttcc
      841 agatcagatc gtagagcttg aactcgtccg gcgagaggtg tgccttcatc tgctgcggcg
      901 tgcggaacac gtcggtcgga cggatggctt cgtgcgcttc ctgagcgttc ttagccttgg
      961 tcgtgtattt gcgcggcgcg gccggcagga atttcgactg ccagttcttg ccgatatagg
     1021 cgcggacctg cgccaccgcc tccggggcca tgtccacgcc gtcggtgcgc atgtaggtga
     1081 tcaggccgac ggtctcgccg ccgatatcgg cgccctcgta gagcttctgg gccacgcgca
     1141 tggtgatctg cgggctcatg ccgagcttgc ggctggcctc ctgctgcagc gtcgaggtcg
     1201 tgaacggtgc gaaggggtgg cgtttcgtcg gcttcgattc gactgaaacg acggaaaact
     1261 tcgccgtttc aaggtcttcc ttgaatttct tcgcgtcgtg cggattgccg atgtcgagcc
     1321 gcgcgatctt cttgccgtcc gcgccgacca gccgcgcatc gaaggcctcg ccggccggcg
     1381 tcagcagtgt cgcgacgatg gaccagtagt cctgccggac gaatttctcg atttccagct
     1441 cgcgatcgca gaccaggcgc agcgccacgg actgcacacg gcctgccgag cgcgcgccgg
     1501 gcagcttgcg ccagagcacg ggggagagcg tgaaaccgac gagataatcc agtgcgcggc
     1561 gggcgagata ggcgtccacc agcgcctgat cgatctcgcg cggcgcggcc atcgcgtcct
     1621 gcacggcctg tttggtgatg gcgttaaagg tgacgcgctg gatcgtcttg cccttcagca
     1681 cgcgcttgtt cttgaggact tccagaatat gccaggaaat cgcctcgccc tcgcgatccg
     1741 ggtcggtggc gaggatcagc cggtccgcac ccttcacggc ggccgcaatg tcggacagcc
     1801 gcttggaggc cttcccgtcc acttcccagg acatggcgaa gtcgttgtcg ggctcgacgg
     1861 aaccgtcctt ggccggcagg tcgcgcacat ggccgaacga ggccagaacc tcaaaatcct
     1921 tgccgagata tttgttgatc gtcttcgcct tggccggcga ttctacgacg acgacgttca
     1981 tctgcgcccc tttaccgatg gaccgcgcca actacgggcc ggggcgtgag atgtcaaatg
     2041 gcgcgccgga aaccgaaatt ttacgcttag tggcaagtct gcacgccatg atgaattcgc
     2101 cccagaggct tcctgtgtcc gagcgaccca actttcggtt cttcatccgc cgcgctggtg
     2161 accctgcacc aacatttcgt gcccgctcgg caagcaatcc gagctacgct tttgattccg
     2221 ccgcaggacg caacatcgtc ctgtgttttt ttggctccac gtcccatgag actgggcgca
     2281 gtgcactaga agcggtggcc cgaaatcgcg cgatcttcga cgacgaccgt ctcgcatttt
     2341 tcggcgtatc cgtagatccg gctgacgagg cccaggggcg catccgagac gcctatcccg
     2401 gcatcagatt tttccacgac catgatggag cgatttgcaa gctctatggc gcgctgcctg
     2461 cccagccgga gggcgtaagg aatttgccct tccgctgcgt gtgggtagtg atagatccaa
     2521 ctctgcgcat cctgaacgta ttgaattttg atcagacggg cgagaaccac gaagacctgt
     2581 ttgcctatct gcgaacgctg ccgcctccgg ggcggcacgc cggaattgag gtgccaacgc
     2641 ccatcctggt ccttccaaac gttttcgatg cagacttctg caagcacctg atcggactgt
     2701 acgacacgca tggcggtgaa gagaccggct tcatgatcga acgcgatgga aagaccgtcg
     2761 gcgtgctcga tcattcgcac aagcgccgcc gtgactacat gattgaagat gacagcatca
     2821 tgcaggaaac tcagaaatgg gttcaacggc ggataatccc ggaaattctc aaagtgcacc
     2881 agttccgggt gacgcgcatg gaacgctata tggtcggctg ttatacggcc gaggatcggg
     2941 gtcatttccg cccccatcgt gacaatacga cgcgtggaac ggcacatcgc cgtttcgcat
     3001 gctcgatcgc gttgaatgac gactacgatg gtggagaagt cggctttcca gaatatggat
     3061 cgcgcacctt caaggcgccg gcaggcgggg cagtcgtgtt ctcctgttcg ttgttgcaca
     3121 tggttacacc ggtaacgcgc ggtcgtcgct atgtattctt gcctttcctc tacgacgacg
     3181 ctgctgctga aattcgcgaa cgcaacaaca cctatctgtc ggaagagatt caggcctacg
     3241 atccgaacaa gaagttcgca gcctaaaagg ctgtcggcac accgcctgct tattcgttgt
     3301 cggcctggat ggcgtcgatc acggcgtccg tgacgctgcg ggtcgtcgcc ttgccgccaa
     3361 gatcgggggt gtggagcgcg ggattggcgg tgacgcgctc gatggcccgc atcagccggc
     3421 cggcggcggc aggttcgcct aggtgctcca gcataatcga ggcggaccag aaggtgccga
     3481 ccggattggc tatgcccttg cccatgatgt cgaaggctga gccatggatc ggctcgaaca
     3541 tcgacgggaa cttgcgttcc ggattgatgt tcgccgtcgg cgcgatgccc agcgatccgg
     3601 caagtgccgc cgccagatcg gacaggatgt cagcatgcag gttggtcgcg acgaccgtgt
     3661 cgatgctcca aggtttcagc gtcatgcgca tggtcatggc gtcgacgatc gccttgtccc
     3721 aggtcacgtc cgggaattcc tgcgccacct cggcggcgat ctcgtcccac atcaccatgg
     3781 cgtagcgctg ggcgttcgac ttggtgacga ccgtcaggag cttgcgcggg cggctctgcg
     3841 ccagtttgaa ggcgaagcgc atgatgcgcg tcacacccgc gcgggtcatg atggacacat
     3901 ccgtggccgc ttcttccggc agcccctggt ggacgcgtcc gccgacgccg gaatactccc
     3961 cctcggagtt ctcacgcacg atcacccagt cgagttcggg gcccgagact gcccgcagag
     4021 gcgaggtgat ccccggaagg atgcgggtcg gccgcacgtt cgcgtactgg tcgaaaggct
     4081 ggcagatggc gagccgcagc ccccacagcg tgatgtgatc ggcaatatca ggatggccgg
     4141 ccgaaccgaa caggatggcg tcatggttcc ggatcagcgc gcggccatcc tccggcatca
     4201 tgcggccatg ctttttgtaa tagtcgccgc cccagtcgaa atgatcgaag cggaaggcaa
     4261 agccaccctc gcgcgccgcc acggcctcaa gcacctcgac accggccgca accacttccg
     4321 tgccgattcc gtcgccggga atggcggcaa tgcgataggt cttcacgtca cgctcctgtc
     4381 ggaaagtcgg ccagccggtc cgtacggtgt cagttggcaa ggcctttcag cgcatcgccc
     4441 cagcgtttca cttccgccgt cagatgcgcc ttgagcgcat cgggctgcgc ggtttccgcc
     4501 gagaccggcg cggtgccaag gctggcgaag cgctcgacca ccttcgggtc cttcaacgca
     4561 gcaagcagcg cggcggacag tttcgccttc acgtcctgcg gcaggttctt cggcgcccac
     4621 atggcgtgcc aggcagaaac ctcaaagcct ttcacgccgc tcgaatccag cgtcggcagt
     4681 tccggcagcg atccgaccgg ggtcttggtc gtcacggcga agcccttgat cttcttgtcc
     4741 ttgatctggt tcgtcgtgtt ggtggtctgg tcgcacatga tgtcgaactg gccgccgagc
     4801 agatcggtca tcgccgggcc ggtgccgctg tatgcgacgc cgcgaagctt gagaccgagt
     4861 tcatgggtca gcatggtcat gcacaaatgc gatgccgagc cgatgccggc gtggccgtag
     4921 ttgaccttgt ccgcatccgc tttcatccag gccagcaact cggccggtgt gttcgcggcg
     4981 agcgtcgcgc gcgagaccag tgccatcggc acatcggtga tgcggccgac atggtcgaaa
     5041 tcatcgacca cattgtaggc gagtgacttg taaagcgccg gggcggtggc gtgggcgatg
     5101 tgccagaccg cgaccgtgta tccgtcgttt gctgccttgg cgacacggcc catgccgatg
     5161 gtgccgccag cccctccggc attctcgacg accacgttct ggccgagcgt cttgcccatc
     5221 gcctcggcga tgaggcgcgc cactgtgtcg gtcggccccc ctgccgccgc aggaacgatc
     5281 aaggtgatcg gccgcgtcgg gaacgcctgt gctgcggcca gtcccggcat agacgcgagg
     5341 gctgcaacga gcgcgccggc cttcagaata ttgcgacgaa acggcatgat gtcctccccg
     5401 atgcggcgcg acttttctgc aatgcccgct atccggacga tcctagcgct ttgcctcttg
     5461 tcacctcaat cgcaacagaa ttatataaaa tgtgcatata atgtctgacg cgcgatggac
     5521 atcacggaac ttcgctgcct cgtcgccctt ggtgacgaat tgcatttcgg ccgtgccgcc
     5581 cagaagctgg acatgctgcc ggcctcgctg gggcgctaca tccgccttct ggaggaggat
     5641 ctcggcgtgc gcctcgtcgc ccgcaccacc cgcagcgtcg cgctgacggc ggccggcgtg
     5701 gaactgctgg aagaggcgcg ccgcctta
//
LOCUS       DAOMXN010000172         5724 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_7451_length_5724_cov_10.989416, whole genome shotgun sequence.
ACCESSION   DAOMXN010000172 DAOMXN010000000
VERSION     DAOMXN010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 5724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5724
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7451_length_5724_cov_10.989416"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="PK812_11030"
     CDS             <1..233
                     /locus_tag="PK812_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HRJ70131.1"
                     /translation="VASFPVENADQIMLVTNGGQLIRCPVDGIRVAGRGTQGVIVFNT
                     ADKDKVVSVERVSDAGEDDSGEDVGESDASAK"
     gene            complement(250..1023)
                     /locus_tag="PK812_11035"
     CDS             complement(250..1023)
                     /locus_tag="PK812_11035"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HRJ70132.1"
                     /translation="MAAFNRETVLSVHHYTDSLFRFETTRDPHFRFENGQFAMIGLEV
                     DGRPLVRAYSMASANYEETLTFFSIKVPNGPLTSRLQHVQPGDTVLIGRKPTGTLVQT
                     SLLPGKRLYLLSTGTGIAPFAGVIKDPDIYDRYESVVLVHGCRLAAETVFGHEVVASV
                     LESEFLGEVARGKLLHYPTTTREHSFHEGRVTDLIETGKLFADLGLPPLDPAQDRVMI
                     CGGPQMLIDLKAMLERRGFSEGSGGAPASYVIEKAFAER"
     gene            complement(1319..3373)
                     /gene="parE"
                     /locus_tag="PK812_11040"
     CDS             complement(1319..3373)
                     /gene="parE"
                     /locus_tag="PK812_11040"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012495963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HRJ70133.1"
                     /translation="MAKAKRAAAEADDLLNKAKSTSKSGSARPPASPAAPTVSAAGSY
                     SAADIQVLEGLEPVRRRPGMYIGGTDEKALHHLFAEVIDNSMDEAVAGHANFIEVELL
                     GDGSLAVTDNGRGIPVDPHEKFPDKSALELIMCTLHAGGKFDSKAYETSGGLHGVGIS
                     VVNALSEITEVEVWRDRQAYRMCFARGVPQGALQHLGPVHNRRGTRVRFKPDDQIFGK
                     DAKFDPRRVFKMARSKAYLFGGVEIRWKCDPTLIKEGANVPESAVFRFPAGLKDYLTR
                     EIEGKDLAADGIFAGKVDKSGHGACEWAVTWFTYDDGFVHSYCNTIPTPDGGTHEAGL
                     RVALLRGLKDHAQRIGASKRASVLTTDDIMVSCAAMLSVFIREPEFVGQTKDKLATLE
                     ASRIVEAAVRDAFDHWLAAAPAAALKLLDFVVERAEDRLRRRAEKDVQRKTAVRKLRL
                     PGKLADCSSTAADGSELFIVEGDSAGGSAKQARDRATQAVLPLRGKILNVANATRDKL
                     TANQQLADLMLALGCGSGSHYKEGDLRYDKIVIMTDADVDGAHIASLLITFFYRQMPK
                     LIDDGHLYLAVPPLYRLTHGGKTVYARDDAHKDQLLATVFKGKKPEIGRFKGLGEMMP
                     AQLKETTMDKKKRTMLQVRIAPDAKPDTADCVERLMGNKPEARFVFIQERAAFAGDLV
                     DL"
     gene            complement(3709..>5724)
                     /locus_tag="PK812_11045"
     CDS             complement(3709..>5724)
                     /locus_tag="PK812_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70134.1"
                     /translation="PGVGNPPGWHPPMAPGVTPAGTTGGMPPFSIGPSTGQERLTTEV
                     TSWTNAAQKTISDNLPSWLPHDVGSLFNSDRGQSLALKVFSPNYDILKGTSLPAPDGA
                     VAPPRSFYTLPKLPGGQDWKGKLHSWIAVRGEVAVSPSWLSASGTEMRTGTFTLTGSA
                     QMEVVSDLPLVKGDIRSLTTGNVGMTQSFSITTNEARLEAIMTGKEPMPNPYTLANMK
                     PGDTVTLFTGPTESAEQEIRVLANKTGRRAGADFKLDATWAQGSKLTVKMVDNPQNAA
                     QPHVIIMDGNTNGSGIAAGIGGIWNPALSSMNKDGTYNRRLDPYANGAFVRVLGQYKE
                     SDTVDTYTFVELNPYDTANGGQALFNQIMNQGYTMDPNAAPAAPIPGATAYGTMAVDT
                     KTAAAALQLTGYWEPNHKLGNNNPQFLGQYSMDLVTMIDSSTSTQVTNADARATVGAS
                     GDLNVSLRYTDGGFTNYFHSYTVNAQKQTSNERYVITAENAFKDQVINGLETGMAQTV
                     RLHLKDGTVVTGPDAVRWLKDHDQGERAFFGLFDKRDKIGVVMDLGTTTEANRFSQQF
                     IADQKKTHDDAKDTPLEWFAYTLGQAKQTEAAFSTAYFSAPDLMMTPDLAFNLAQHQD
                     AQSAHENFRVMAGEGMGNLIDSMGDYYTHNQRTTPYPVSIDIYVDFD"
BASE COUNT          950 a         1756 c         1920 g         1098 t
ORIGIN      
        1 tggtggcttc cttcccggtg gagaacgccg accagatcat gctggtgacc aacggcggcc
       61 agctcatccg ctgcccggtg gacggcatcc gcgtcgccgg gcgcgggaca cagggcgtca
      121 tcgtcttcaa caccgccgac aaggataagg tcgtctcggt cgagcgcgtg agcgacgcgg
      181 gcgaggatga ctccggcgag gacgtgggcg agagcgacgc gtcggcgaaa tagcgccggc
      241 gccgggaact cagcgctcgg cgaaggcctt ttcgatgacg tagctcgcgg gcgcgccgcc
      301 gctgccttcg gaaaagccgc gtctttcaag catcgccttg aggtcgatca gcatctgcgg
      361 tccaccgcag atcatcaccc ggtcctgggc cggatcgagc ggcggcaggc caagatcggc
      421 aaacagcttg ccggtctcga tcaggtcggt gacgcggccc tcgtggaagg agtgctcgcg
      481 cgtggtggtc ggatagtgca gcagcttgcc gcgcgccacc tcgccaagga actcgctctc
      541 cagcaccgag gccaccacct cgtggccgaa gacggtttcc gccgccagcc ggcagccgtg
      601 cacaagcacc accgattcat agcggtcgta gatgtccggg tccttgatga cgccggcgaa
      661 gggggcaatt ccggtgccgg tggagagcag gtagagccgc ttgcccggca gcaggctggt
      721 ctgcaccagc gtgcccgtgg gcttgcggcc gatcagcacg gtgtcgcccg gctggacatg
      781 ctggagccgc gatgtcagcg gcccgttcgg aaccttgata gagaagaacg tcagggtctc
      841 ctcgtagttg gccgaggcca tcgagtaggc gcgcaccagc ggcctgccgt cgacctccag
      901 cccgatcatg gcgaattgac cgttctcgaa gcggaagtgc ggatcgcgtg tcgtctcgaa
      961 acgaaagagg ctgtcggtgt agtggtggac cgacagcacc gtctcgcgat tgaaggcggc
     1021 cagggctttc gatggcgcgg cggccagcat tggggttggg gcggtgtcga gcatggtatc
     1081 ccctcggtcg gtcaaactgc ttgaaacttc aatcattgaa acttcaagca atctgtcaag
     1141 cgccacgctc tatatgagcg atcgctaatt caacggagcg aataggggat gcgggagggg
     1201 ccgcgctttg atttttgtca aaggaaggac ggggtgaaca ctcctgcggc gttagacgcg
     1261 aagctgcctt tcatggccac gcgccagacc atgcgagccc acgcagcgcc gacggccctc
     1321 acaaatccac caggtcccct gcgaaggccg cgcgttcctg aatgaacaca aaccgcgctt
     1381 ccggcttgtt gcccatcagg cgttcgacgc aatctgcggt gtcaggcttg gcgtccggcg
     1441 cgatgcggac ctgcagcatc gtgcgcttct tcttgtccat ggtcgtttcc ttcaactgcg
     1501 ccggcatcat ttcgccgagg cccttgaagc ggccgatctc cggtttcttg cccttgaaca
     1561 cggttgcgag cagctggtcc ttgtgggcgt cgtcgcgggc gtagaccgtc ttgccgccgt
     1621 gggtgaggcg gtagagcggc ggcacggcga ggtagaggtg cccgtcgtcg atcagcttgg
     1681 gcatctgccg gtagaagaag gtgatgagga gcgaggcgat atgcgccccg tccacatccg
     1741 cgtcggtcat gatgacgatc ttgtcgtagc gaaggtcgcc ttccttgtag tgcgagccag
     1801 agccgcagcc gagcgcaagc ataaggtctg cgagttgctg gttggcggtc agcttgtcgc
     1861 gcgtggcgtt ggcgacgttg aggatcttgc cgcgcaaggg cagcacggcc tgcgtcgccc
     1921 ggtcgcgcgc ctgcttggcc gagccgccgg ccgaatcgcc ctcgacgata aagagttccg
     1981 agccgtcggc ggcggtggag gagcaatccg cgagtttgcc cggcaggcgc agcttgcgca
     2041 cggcggtctt gcgctgcacg tccttctcgg cgcggcggcg cagacggtct tcggcccgtt
     2101 cgacgacgaa atccagaagc ttgagcgcgg cggctggcgc ggcggccagc cagtggtcga
     2161 aggcgtcacg cacggcggcc tcgacgatcc tggaggcttc cagcgtcgcc agcttatcct
     2221 tggtctggcc gacgaactcc ggctcgcgga tgaagacgga gagcatcgcg gcgcaggaca
     2281 ccatgatgtc atcggtggtc agcacagagg cgcgcttgct cgccccgatg cgctgggcat
     2341 ggtccttcag gccgcgcaac agcgcgacac gaagccccgc ctcgtgcgtg ccgccatcag
     2401 gagtgggaat ggtgttgcag taggagtgga cgaagccgtc gtcgtaggtg aaccaggtga
     2461 ccgcccattc gcaggcgccg tgaccggact tgtccacctt gccggcaaag atgccatcgg
     2521 cggccaggtc cttgccttcg atctcgcgcg tcagatagtc cttaagcccg gccggaaagc
     2581 ggaagaccgc cgactccggc acgttggcgc cttccttgat cagggtcgga tcgcatttcc
     2641 agcggatctc aaccccgccg aacagatagg ccttcgagcg ggccatcttg aatacgcggc
     2701 gcgggtcgaa cttcgcatcc ttgccgaaaa tctggtcgtc cggcttgaag cgcacccgcg
     2761 tgccccgccg gttgtggacg gggcccagat gctggagcgc gccctgcggc acaccccgtg
     2821 cgaagcacat gcgataggct tgccggtcgc gccagacctc cacctcggtg atctccgaca
     2881 gagcgttcac caccgatatg ccgacgccgt gcaggccgcc tgatgtctcg taggccttgg
     2941 agtcgaactt gccgccggcg tgcagcgtgc acatgatcag ttcgagcgcg gacttgtccg
     3001 gaaacttctc gtgcggatcg acgggaatgc cgcgcccgtt gtcggtcaca gccagcgagc
     3061 catcgccaag aagctcgacc tcgatgaagt tggcgtggcc agccaccgcc tcgtccatgg
     3121 aattgtcgat cacctcggcg aagagatggt gcagcgcctt ctcgtccgtg cccccgatgt
     3181 acatgcccgg ccggcgacgc accggctcaa gcccttccag aacctggatg tcggcggcgg
     3241 aatagctgcc ggcggcggac actgtcggcg cggcgggcga ggcggggggc cgggcgctgc
     3301 ctgacttgct ggtcgatttc gccttgttca gaagatcgtc cgcctcggcc gccgcgcgct
     3361 tggctttggc cagcgccaat tccgcccgcg cgcgatcatc ggccgtcgcc ttgccaacaa
     3421 gcgacgaggc ggccttctcc agttccccga acagatcgcg tgccggcccg gatttcgacg
     3481 acatgcccgc tcttcccaca aagtgattcg caagcgccaa gtatgccatg attgaccgac
     3541 gcacggtgtc ttcgtcaccg gggctggacc acgcagccta ggacgccgct gacgggcttg
     3601 aacctactgg gcaaggactt ccgctggcac gccgaagcgc tcggacggga cacgggaaac
     3661 agtccgggtt ccgcatcgga cgagatcggc ccaggacttc ggcgtacttc agtcgaaatc
     3721 gacgtagatg tcgatgctga ccgggtaggg cgtggtgcgc tgattatggg tgtagtagtc
     3781 ccccatcgag tcgatgagat tgcccatgcc ttcgccggcc atgacccgga aattctcatg
     3841 ggcggactgc gcatcctgat gctgcgcgag attgaaggcg agatcagggg tcatcatcag
     3901 gtcgggcgcc gagaaatagg cggtgctgaa ggcagcttcg gtctgtttgg cctgaccgag
     3961 ggtataggcg aaccactcca gcggggtgtc cttggcgtcg tcgtgggtct tcttctggtc
     4021 ggcgatgaac tgctgggaga agcggttggc ttccgtcgtg gtgccgaggt ccatgacgac
     4081 gccgatcttg tcgcgcttgt cgaagagacc gaagaatgcg cgctcgccct ggtcgtgatc
     4141 tttaagccaa cgcacggcat ccggtccggt gacgacggtg ccatccttga gatgcagacg
     4201 cacggtctgg gccatgcctg tttcgaggcc gttgatcacc tgatccttga aggcgttctc
     4261 ggccgtgatg acatagcgct cgttgctcgt ctgcttctgc gcgttgacgg tgtagctatg
     4321 aaagtagttg gtgaatccgc cgtccgtgta gcgcagggac acgttgagat cgccgctcgc
     4381 gccgaccgtc gcgcgggcat ccgcgttggt gacctgggtg ctggtggatg aatcgatcat
     4441 cgtcaccaga tccatggaat actgtcccag gaactgtgga ttgttgttgc cgagcttgtg
     4501 gttcggttcc cagtagccgg tcagttgcag ggcggcggcg gcggtcttgg tgtcaaccgc
     4561 catcgtgccg taagccgtcg caccggggat cggcgcggcc ggggccgcgt tggggtccat
     4621 cgtgtagccc tggttcatga tctggttgaa cagggcctgc ccgccattcg cggtatcgta
     4681 gggattaagc tccacaaaag tgtaggtgtc gacggtatcg ctctccttgt actgaccgag
     4741 cacgcgcacg aaggcgccgt tggcataagg atcgaggcga cgattgtagg tgccatcctt
     4801 gttcatggaa ctgagcgccg ggttccagat gcccccgatg cctgcggcga tccccgagcc
     4861 gttggtattc ccgtccatga tgatgacatg cggctgcgcc gcgttctgcg gattgtcgac
     4921 catcttgacc gtgagcttgc tgccctgcgc ccaggtcgcg tccagcttga agtctgcgcc
     4981 ggcgcgccgt ccggtcttgt tggcgaggac gcggatttcc tgttccgccg attccgtcgg
     5041 ccccgtgaac agggttaccg tatcgcccgg tttcatgttc gccagcgtat aggggttggg
     5101 catgggctcc ttgccggtca tgatcgcctc gaggcgcgcc tcgttcgtgg tgatcgagaa
     5161 gctctgcgtc atgccgacgt tgcccgtggt gagcgagcgg atgtctccct tcaccaaggg
     5221 gagatcgcta acgacctcca tttgcgcgct gccagtgagt gtaaatgtgc cggtgcgcat
     5281 ctccgtgccg cttgccgaaa gccaggacgg gctgaccgcg acctcgccgc gtaccgcgat
     5341 ccaggaatgc agcttgcctt tccagtcctg cccaccgggg agcttcggca atgtgtagaa
     5401 tgatcgtggc ggcgcgaccg ccccgtcggg agccggaagc gacgtgcctt tcaggatgtc
     5461 gtagttcggg gagaatacct tgagcgccag cgactggccg cgatccgaat tgaacagtga
     5521 gccgacgtca tgcggcagcc aggatggcag gttgtcgctg atcgtcttct gggctgcgtt
     5581 cgtccagctt gtgacctcgg tcgtcaaccg ctcctggccg gttgagggac cgatggagaa
     5641 gggaggcatt ccgcctgtcg taccggctgg ggtcacgccc ggcgccatcg gcggatgcca
     5701 gcccggtgga tttccaacgc ctgg
//
LOCUS       DAOMXN010000173         5689 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_7497_length_5689_cov_10.824459, whole genome shotgun sequence.
ACCESSION   DAOMXN010000173 DAOMXN010000000
VERSION     DAOMXN010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7497_length_5689_cov_10.824459"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            406..1326
                     /locus_tag="PK812_11050"
     CDS             406..1326
                     /locus_tag="PK812_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HRJ70135.1"
                     /translation="MKRRFYTLDVFTERKLAGNPLAVVLDCEGLDDASMQAIAREFNL
                     SETVFVSPPADPVNTAAVRIFTPGGELPFAGHPTVGTGVLLAQLEAPEMVAASGGIVV
                     ALEEKIGVIKVEVSAQNGRVARGIFRVPRLSETVPWTPDLPLAAAGLGLDPLDIGFGA
                     HALSCWTAGVPFVMCPVKGLEAIRRARVADMDAWRRAFGFNGKGAVFVYTSETENPQH
                     HVHARMFSPMMGIPEDPATGSAAAAFAGPAVIFERPEDGWHQLVIEQGYEMGRPSEIV
                     LDVDVTGFRLSDARIGGGAVIVSQGVLDIA"
     gene            1323..2048
                     /locus_tag="PK812_11055"
     CDS             1323..2048
                     /locus_tag="PK812_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRJ70136.1"
                     /translation="MSADRKPIRQIVDLDRVDASFEAFDWEAPRLHAEAIDAYWEQAV
                     AAAPAIFNGTVLVQHKGLVEGRVFRAAYSPTSYKDFLGYMRLPIPESNVRNGFAMAAL
                     RSRDDAFLLGQMSANTANGGKIYFAAGTPDMDDVRNGRVDLDGSVLREMSEETGLRLE
                     EVTIGAGWTCLIAEKRAAFMRDVFLDLPAKEARALMLGRMKTLHEQELSDIVIVRAGD
                     PLLDDPRLPVFVSEYIRRWYARR"
     gene            complement(2071..2418)
                     /locus_tag="PK812_11060"
     CDS             complement(2071..2418)
                     /locus_tag="PK812_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70137.1"
                     /translation="MSIATRLLPMTAAALLLAACNQSTQQAAAPQAPAQPSFVTPSTF
                     RMPDGAGCTGAVNRYRAIMNNDLETGHVNKSVYEKITKEIDAAAQMCASGNDAGARAA
                     ISASRARHGYPAG"
     gene            complement(2487..3362)
                     /locus_tag="PK812_11065"
     CDS             complement(2487..3362)
                     /locus_tag="PK812_11065"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRJ70138.1"
                     /translation="MTELKHNTVRLNGIDIHYVEAGQGPLILFVHGFPDFWRVWIDQL
                     KHFSKSHRVVALDMRGFNLTSKPKEPEAYRAKILVEDVRQLILHLGERRCILVAHDWG
                     GAAIWNFAAQHPDMVEKLIIMNSPHTMTFVRELVNNPDQQKASAYMNFFRRPEAEQAM
                     TEDNFRRLFSMFGRGNLRTLDDEEKQAYVDAWSTPDGVAGGLNYYRATPMYPPLPNDP
                     GPAALTLDPAQFRVPAPTLVLWGMADTALLPGLLTGLGEHVPNLKVVEMPGVSHWPMR
                     EKPDEVNRLIGAFIG"
     gene            complement(3437..4192)
                     /locus_tag="PK812_11070"
     CDS             complement(3437..4192)
                     /locus_tag="PK812_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRJ70139.1"
                     /translation="MAFALSDVSLAQSALVASTAFVASIIGGVAGYGTGLLLPLALVP
                     IIGAEATVPVIAISALFTNASRALALRRSIDWSRFRLMLPAALPMTMLAATLFTRLDN
                     RGAAIVIGCVLIALVPLRHWLKRAGFRLQGRALAAGGGIYGFVTGGSTGAGVILVSFL
                     MASGLTGAAVVATDAAISIVIGAAKAATFGALGALPGPLLVFALLVGLSTVPGAFIAR
                     HILERLSLKIQTALLDAVVVIGGAVMVVRALLA"
     gene            complement(4211..5260)
                     /locus_tag="PK812_11075"
     CDS             complement(4211..5260)
                     /locus_tag="PK812_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014283666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HRJ70140.1"
                     /translation="MPDSKVRYIDVVDMHTAGEPVRIVTGGYPVLNGASILDKRRDAL
                     ANHDAFRRALMLEPRGHAGMYGVIPVQPSLPGADFACLFMHNEGYSTMCGHATIALGK
                     WLVESGHVPRSEPETRFGLELPCGLVEVTAKVVDGKVKESAFVSVPAFLAARDVTVDV
                     PGFGPVTGDLCYGGAYYLVLPARAAGLDFHAAPLAALVAAGTQITEAGRAQLSVDHPE
                     DRDLGFLYGTILTDDAPAREASYNLCIFADGQIDRSPTGSGVTARMARDHARGLIAPG
                     VKREFYGPTPVPFSAEIASTVNGGRDGAVRVRVSGTSAYCGSARFELHADDPLAWGFL
                     LPETYGDLRAATPSS"
     gene            complement(5314..>5689)
                     /locus_tag="PK812_11080"
     CDS             complement(5314..>5689)
                     /locus_tag="PK812_11080"
                     /inference="COORDINATES: protein motif:HMM:NF017273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HRJ70141.1"
                     /translation="ACCRAAGARSSPASPAASSPGYAMTQPSDFWLLLAGATLVSFAC
                     RAGGFWLMRFVSITPRLEAALRATPLAVMVGIATPAATSGRPAEILAILVTIVLMRAT
                     RNDLAAALGGVAVLAVLRQVLA"
BASE COUNT          969 a         1795 c         1999 g          926 t
ORIGIN      
        1 gggcggccgg agcggccggg cgctgcgggg ccggctgctg ctgcgtcgcc tgagcgaggg
       61 ctgcggtgcc ggtggcgaac aggatggcca gagcggcggc ggttgtcttg aatgtcatgt
      121 cacactctcc ttgagtgcat ggcccggttg atggcggcgt ctccgggcgg gagatgcgcc
      181 gcatgcgaat ctggagaata cccccaaaaa aggcccggga ttgccggtcg gccagaatcc
      241 cgaaaggcct catcgctaat ctgacaacta accttgcatc tgtgaaccta tttcgaatgc
      301 gctattgggc ggttgttcac actcgcggcc tgcgctcagg gccttgcgag gcccgtctgg
      361 ccacgagggc tggttccggc ctacaaacgc cggcaggaga aacccatgaa gcggcgattc
      421 tacacgctgg acgtgttcac cgagcgcaag ctggccggca atccgctggc cgtggtgctc
      481 gattgcgagg ggcttgacga tgcgtcgatg caggccatcg cgcgggagtt caacctgtcc
      541 gagaccgtgt tcgtctcgcc ccctgctgat ccggtgaata cggccgccgt ccgcattttc
      601 actccgggcg gtgaacttcc tttcgccggg cacccgacgg tgggcacggg cgtgctgctg
      661 gcgcaactcg aagcgccgga gatggtcgcg gcgtcaggcg gcatcgtggt ggcgctggag
      721 gagaagatcg gcgtcatcaa ggtcgaggtt tcggcgcaga acggcagggt ggcgcgtggc
      781 atcttccggg tgccaaggct gtccgagacg gtgccctgga cgcccgacct gccgcttgcc
      841 gctgctggtc ttggcctcga cccgctggac atcgggtttg gggcccatgc gctcagttgc
      901 tggacggccg gcgtgccgtt cgtcatgtgt ccagtgaaag gacttgaggc gatccgacgt
      961 gcgcgcgtcg cggacatgga tgcctggcgc cgggctttcg gattcaacgg caagggtgcg
     1021 gtgttcgtct acacctcgga gaccgagaac ccgcagcacc acgtccatgc ccgcatgttc
     1081 tcgccgatga tgggcatccc ggaagacccg gcgaccggtt cggccgcagc ggccttcgca
     1141 gggccggcgg tgatcttcga gaggcccgaa gacggctggc accagctggt catcgaacag
     1201 ggctatgaaa tgggacgtcc gtccgaaatc gtgctcgacg ttgacgtgac gggattccgg
     1261 ctgtcggatg cccggatcgg cgggggcgcc gtcatcgtct cgcagggcgt gctggatatc
     1321 gcatgagcgc ggatcgcaaa cccatccgcc agatcgtcga tctcgatagg gtcgacgcca
     1381 gcttcgaagc cttcgactgg gaagcgccgc gtctccatgc ggaggcgatt gatgcctatt
     1441 gggagcaggc cgtcgccgcg gcgcccgcga tcttcaacgg cacggtgctg gttcagcaca
     1501 agggccttgt cgagggccgc gtgttcaggg ccgcctacag cccgaccagt tacaaggact
     1561 ttctcggcta catgcggctg ccgatccccg aatcgaacgt gcgcaacggt ttcgccatgg
     1621 cggcgctgcg ctcgcgtgac gacgcctttc tcctgggcca gatgtccgcg aacaccgcca
     1681 acggcggcaa gatctacttc gccgccggca cgcccgacat ggacgacgtg aggaatggtc
     1741 gggtcgacct cgacggcagc gtgctgcgcg aaatgtcgga ggaaaccggc ctgcgcctcg
     1801 aagaggtgac aatcggggcg ggctggacct gcctgatcgc cgaaaagcgc gcggcgttca
     1861 tgcgcgacgt attcctcgac ctgccggcga aggaagcgcg cgccctgatg ctcgggcgca
     1921 tgaagacgct gcacgagcag gagctgtccg acatcgtgat cgtgcgggcg ggcgatccgc
     1981 tgcttgacga tccgcggttg ccggtcttcg tctcggagta catccggcgc tggtacgcgc
     2041 gacgctaggc gacgttcgcc ggaccgctcc tcaccccgcc gggtaaccgt ggcgggcgcg
     2101 gctggcactg atcgcggccc tggcgccggc gtcgttgccg gaggcgcaca tctgcgcggc
     2161 agcgtcgatt tccttggtga tcttctcgta gaccgacttg ttgacgtggc cggtttcgag
     2221 gtcgttgttc atgatcgcgc gatagcggtt gaccgctccc gtgcatcccg cgccgtccgg
     2281 catgcggaag gtcgagggcg tcacgaagga tggctgcgcc ggggcctgcg gggcggctgc
     2341 ctgctgggtc gactggttgc atgcggccag aagcagcgcg gcggcggtca tcggcaacag
     2401 gcgggtagcg atgctcatga gggcctcgtc aaggtgatgt ctgcagaaca gattgaagat
     2461 tccggatcgc aggtcaacgg ccctgcctag ccgatgaacg cgccgatcag gcggttcacc
     2521 tcatccggct tttcccgcat cggccagtgg ctgacgccgg gcatctcgac gactttcagg
     2581 ttcgggacgt gctcgccgag gccggtcagc agccccggca gcagcgcggt gtccgccatg
     2641 ccccacagca ccagcgtggg ggccgggaca cggaattgcg ccggatcgag cgtgagggcg
     2701 gccggtccag gatcattggg tagcggggga tacatcggcg tcgcgcggta gtagttcagg
     2761 ccgccggcga ccccgtccgg cgtcgaccat gcatccacgt aggcctgctt ttcctcgtca
     2821 tcgagggtgc gcaggttgcc gcggccgaac atcgagaaca gacgccggaa gttgtcttcc
     2881 gtcatcgcct gctccgcctc cggacggcgg aagaaattca tgtaggccga ggccttctgc
     2941 tggtccggat tgttcaccag ttcgcgcacg aaggtcatgg tgtgcggcga gttcatgatg
     3001 atgagctttt ccaccatgtc cgggtgctgg gcggcgaaat tccagatggc cgcgccgccc
     3061 cagtcatgcg cgaccaggat gcagcgccgc tcgccgagat gcaggatgag ctgacggacg
     3121 tcttcgacca ggattttcgc gcggtaggct tccggctcct tcggcttcga ggtgagattg
     3181 aagccgcgca tgtccagcgc gacaacgcga tgcgacttgg aaaagtgctt gagctggtcg
     3241 atccacacgc gccagaaatc cgggaagccg tgcacgaaca ggatcaacgg gccctggccg
     3301 gcttcaacgt agtggatgtc gatgccgttc agccgaacgg tgttgtgctt gagttcggtc
     3361 acgctgtccc ctccgatgcc tgatcgcgcg gcatcatcgg cggaaccgtc agccctgaca
     3421 atggccttgt gagggatcag gccagtaagg cgcgcacaac catcaccgct ccaccgatca
     3481 ccacaacggc gtcaagcagc gccgtctgga tcttcagcga gagccgttcg aggatgtgcc
     3541 tggcgatgaa ggcgccggga acggtcgaca gtccgacaag cagggcgaag accagtagcg
     3601 gacccggcag agcgccgaga gcgccgaacg ttgcagcctt cgcagcgccg atgacgatgg
     3661 aaatggccgc atccgttgcc acgacggcag cgccagtcaa tccggaggcc atcaggaagg
     3721 agaccaggat gacgccggcg cctgtggacc caccggtgac aaagccatag atgccgcctc
     3781 ccgccgccag cgcgcgcccc tgcaagcgga agccggctcg cttcaaccaa tggcgcaggg
     3841 gcaccagcgc gatcagcacg caaccgatga caatggctgc gccccggttg tcgaggcggg
     3901 taaacagtgt cgccgccagc atggtcatag gcagggcggc gggcagcatc aggcgaaagc
     3961 ggctccagtc gatggaacgt cgcagggcca gtgcccggct ggcattcgtg aacagggccg
     4021 atatggcgat gaccggcacg gttgcctcgg caccgatgat cggcaccagc gccagcggca
     4081 ggagcaggcc ggtgccatat ccggcgacgc cgccgatgat tgaggcgaca aaggcggtgg
     4141 atgctaccag cgcggactgc gccagcgaga cgtcagacag tgcgaaggcc atgcctcagg
     4201 ccccggagcg tcatgaagac ggcgtggcgg cgcgcaggtc gccgtaggtt tcgggcagca
     4261 ggaagcccca ggcgagagga tcgtccgcat gcagctcgaa ccgggcggaa ccgcagtagg
     4321 ccgacgtgcc ggagacgcgc acgcgcacgg caccgtcgcg ccctccgttc accgtggacg
     4381 cgatctccgc gctgaatggc acgggcgtcg gaccgtagaa ctcgcgcttc acgccgggcg
     4441 cgatgaggcc gcgcgcgtgg tcgcgcgcca tgcgcgcggt aacgccgctg ccggtggggc
     4501 tgcggtcgat ctggccatcg gcgaagatgc agaggttata gcttgcctcg cgcgccgggg
     4561 cgtcgtcggt gaggatggtg ccgtagagaa agccgaggtc gcgatcctcc gggtggtcga
     4621 cactcagctg ggcgcgtccc gcttccgtga tctgcgtgcc ggcggcgaca agggcggcga
     4681 gcggggcggc atggaaatcc agtccggctg cgcgggccgg caagacgaga tagtaggcgc
     4741 cgccgtaaca caggtctcct gtcaccggac cgaagcccgg cacatccacc gtcacgtcgc
     4801 gggcggcgag aaaggcgggc acgctgacga aagcggattc cttcaccttg ccgtcgacga
     4861 ccttcgcggt gacctccacg agcccgcagg gcagttcgag gccaaagcgc gtttcaggct
     4921 cggagcgtgg cacgtgaccg ctctcgacga gccacttgcc gagtgcgatc gtggcgtggc
     4981 cgcacatggt gctgtagccc tcgttgtgca tgaacaggca ggcgaaatcc gcgcccggca
     5041 ggctcggctg cacggggatg acgccataca ttccggcatg gccgcgcggc tcaagcatca
     5101 gggcgcggcg aaaggcatcg tggttggcga gcgcatcgcg ccgcttgtcc aggatgctgg
     5161 cgccgttcag cacgggatag ccgcccgtga cgatgcgcac cggctcgccc gcagtgtgca
     5221 tgtcgacgac atcgatatag cgcactttcg aatccggcat ggtccgctcc tttggcctgc
     5281 aagggtcgca tccggcgcgc gggcgatcaa ggttcaggcg agcacttgcc tgagcaccgc
     5341 cagcaccgcg acgccgccca gcgccgcggc cagatcgttg cgtgtcgcac gcatcaggac
     5401 gatggtcacc aggatcgcca gtatctctgc ggggcgcccg ctggtggcgg ctggcgtggc
     5461 gatgccgacc attacggcca gcggtgtggc gcgcagcgcg gcttccagcc tcggggtgat
     5521 gctcacgaag cgcatcagcc agaagccgcc ggcgcgacag gcgaacgaca cgagcgtggc
     5581 accggccaga agcagccaga agtcactcgg ctgggtcatg gcgtatccag gcgatgaggc
     5641 cgccggcgat gccggcgagg accgtgctcc agccgcccgg cagcaggcg
//
LOCUS       DAOMXN010000174         5683 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_7511_length_5683_cov_11.980100, whole genome shotgun sequence.
ACCESSION   DAOMXN010000174 DAOMXN010000000
VERSION     DAOMXN010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 5683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5683
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7511_length_5683_cov_11.980100"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..1936)
                     /gene="dnaK"
                     /locus_tag="PK812_11085"
     CDS             complement(17..1936)
                     /gene="dnaK"
                     /locus_tag="PK812_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HRJ70142.1"
                     /translation="MGKVIGIDLGTTNSCVAVMEGTTPKVIENSEGARTTPSIVAFTD
                     EGERLVGQPAKRQAVTNPSRTFFAIKRLIGRTYADPMTQKDKGLVPYAITKAGNGDAW
                     VEADGKTYSPSQISAFTLMKMKETAEAHLGQPVTQAVITVPAYFNDAQRQATKDAGKI
                     AGLEVLRIINEPTAAALAYGLDKKKAGTIAVYDLGGGTFDVSILEIGDGVFEVKSTNG
                     DTFLGGEDFDMRLVEYLADEFKKEQGIDLKKDKLALQRLKEAAEKAKIELSSAAQTEI
                     NLPYITADASGPKHLTLKLSRSKFEQLVDDLIQRTVEPCKKALKDAGLSAGQIDEVVL
                     VGGMTRMPKVQEVVKQFFGKEPHKGVNPDEVVAIGAAVQAGVLQGDVKDVLLLDVTPL
                     SLGIETLGGVFTRLIDRNTTIPTKKSQVFSTAEDNQQAVTIRVFQGEREMAADNKALG
                     QFDLMGIPPAPRGMPQIEVTFDIDANGIVSVTAKDKATNKEQQIRIQASGGLSDADIQ
                     KMVKEAEANAAEDKKRRELVEAKNQAEAMIHSTEKSVKEYGDKVSTSDKEAIENAITA
                     LRDTLGGEDIDLLQARTNDLMQASMKLGEAMYKAQADAETKTAEADAARDAKKAGEDV
                     IDADFKEVKKDDKKS"
     gene            complement(2185..3849)
                     /locus_tag="PK812_11090"
     CDS             complement(2185..3849)
                     /locus_tag="PK812_11090"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HRJ70143.1"
                     /translation="MTRIDNARVIRAPRGPDISAKSWLTEAPLRMLMNNLDPDVAEKP
                     GELVVYGGIGRAARDWESFDRIAAALRRLEADETLLVQSGKPVGVFRTHADAPRVLIA
                     NSNLVPRWATWEHFNELDRRGLMMYGQMTAGSWIYIGSQGIVQGTYETFVEMGRRHYN
                     GDLSGRWILTAGLGGMGGAQPLAAVMAGACCLAVECRPSSIEFRLRTGYVDVLAKDLD
                     DALAIIAKATKEKKALSVALLGNAADVFPELVRRGVRPDCVTDQTSAHDPVNGYLPKG
                     WTLADWEERRTSDPKGVTAAAKKSMAEHVRAMLDFHHRGVPTVDYGNNIRQMALEEGI
                     ADAFDFPGFVPAYIRPLFCRGVGPFRWAALSGDPEDIYRTDAKVKELLPDNKHLHNWL
                     DMAQKRIKFQGLPARICWVGLGDRHRLGLAFNEMVAKGELKAPIVIGRDHLDSGSVAS
                     PNRETEAMRDGSDAVSDWPLLNALLNCASGATWVSLHHGGGVGMGFSQHSGMVIVCDG
                     TPEASKRIERVLWNDPATGVMRHADAGYDIAVDCARTHGLNLPSLA"
     gene            complement(3854..4684)
                     /gene="hutG"
                     /locus_tag="PK812_11095"
     CDS             complement(3854..4684)
                     /gene="hutG"
                     /locus_tag="PK812_11095"
                     /EC_number="3.5.1.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009489345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050129 - N-formylglutamate deformylase
                     activity [Evidence IEA];
                     GO_process: GO:0019557 - histidine catabolic process to
                     glutamate and formate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate deformylase"
                     /protein_id="HRJ70144.1"
                     /translation="MSAIPDWLTVRRGQAPLIVSLPHTGTSIPPDIEARLVSPWLARK
                     DADWWIDRLYDFAADLDATVVHTAVSRTVIDVNRDPSGVSLYPGQATTELCPSTTFDG
                     ESLYRDGHAPDGAEIAARRARWFDPYHAVLAGEIARLRTRHERLVLYDCHSIRSRIPR
                     LFEGDLPQFNIGTNSGAACAETLQTVIARICAETGLDHVVNGRFKGGYITRCYGAPER
                     GVHAVQMELACRGYMHETPGPVGAADWPAPYDPAFAAPMRGHLARILSACLLFAETKD
                     "
     gene            complement(4681..>5683)
                     /locus_tag="PK812_11100"
     CDS             complement(4681..>5683)
                     /locus_tag="PK812_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009489347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HRJ70145.1"
                     /translation="RPLELGPKEGLALLNGTQFSTANALAGLFAAEDLFRTALVAGAL
                     STDAARGSDAPFDPRIHLLRRHRGQMETAATLRALMAGSAIRASHLVGDERVQDPYCL
                     RCQPQVMGAVLDLLRQAAETLATEANGVSDNPLILSDTGEALSGGNFHAEPVAFAADM
                     IAMAVCEIGSLSERRIAMLVDPALSGLPAFLTPKPGLNSGFMIPQVTAAALVAENRQM
                     AFPASVDSIPTSANQEDHVSMAAHGARRLARMVENARAIVAIELLAAAQACDFHAPLR
                     SSHRLEAVRMVLRAHVDHLDDDRHFHPDIEAALALVRRGDLVRTAGEDIFPKLGADA"
BASE COUNT          907 a         1837 c         1923 g         1016 t
ORIGIN      
        1 gggcgggagg atcacgtcac gatttcttgt cgtccttctt cacttccttg aagtcggcat
       61 cgatcacgtc ctcgccggcc ttcttggcgt cgcgggcggc atcggcctcg gcggtcttcg
      121 tctccgcgtc cgcctgagcc ttgtacatgg cctcgccgag cttcatcgag gcctgcatca
      181 ggtcgttggt gcgggcctgc aacagatcga tgtcctcgcc gccgagcgtg tcgcgcaagg
      241 cggtgatggc gttctcgatc gcctccttgt cggaggtcga gaccttgtcg ccgtactcct
      301 tcacggattt ctccgtggag tggatcatcg cctcggcctg gttcttggcc tcgacgagtt
      361 cgcgacgctt cttgtcctcg gccgcgttgg cctcggcctc cttcaccatt ttctggatgt
      421 cggcgtcgga caggccgccc gaagcctgaa tgcggatctg ctgttccttg ttggtggcct
      481 tgtccttcgc cgtcaccgaa acgatgccgt tggcgtcgat gtcgaaggtc acctcgatct
      541 gcggcatgcc gcgcggtgcc ggcggtatgc ccatcaggtc gaactggccg agcgccttgt
      601 tatcggcggc catctcacgt tcgccctgga agacgcggat ggtcaccgcc tgctggttat
      661 cctcggcggt cgagaacacc tggctcttct tggtcgggat cgtggtgttg cggtcgatca
      721 ggcgggtgaa gacgccaccc agcgtttcga tgcccagcga aagcggggtc acgtcgagca
      781 agagcacatc cttgacgtcg ccctgaagca cgccggcctg caccgccgcg ccgatggcga
      841 cgacctcgtc cgggttgacg cccttgtgcg gttccttgcc gaagaactgc ttcacgacct
      901 cctgcacctt cggcatgcgg gtcatgccgc cgacgagcac gacctcgtcg atctggcctg
      961 ccgacaggcc ggcgtccttg agcgccttct tgcagggctc gacggtgcgc tggatcaggt
     1021 catccacgag ctgctcgaac ttggagcgcg acaacttgag ggtcaggtgc ttcgggccgg
     1081 aggcatccgc cgtgatgtac ggcaggttga tctcggtctg cgctgcggac gacagttcga
     1141 tcttggcctt ctcggccgct tccttcagac gctggagggc gagcttgtcc ttcttcaggt
     1201 cgatgccctg ctccttcttg aactcgtcgg cgagatactc gacgaggcgc atgtcgaagt
     1261 cctcgccacc gaggaacgtg tcgccgttgg tcgacttcac ttcgaacacg ccgtcgccga
     1321 tctcgaggat cgagacgtcg aaggtaccgc cgcccaggtc atacaccgcg atggtgccgg
     1381 ccttcttctt gtcgaggccg taggcgagcg cggccgccgt cggctcgttg atgatgcgca
     1441 gcacctcgag gccggcgatc ttgccagcgt ccttggtggc ctggcgctgg gcgtcgttga
     1501 aatacgccgg gacggtgatg acggcctgcg tcacgggctg gccgagatgc gcctcggccg
     1561 tctccttcat cttcatcagc gtgaaggcgg agatctgcga gggggaatag gtcttgccat
     1621 ccgcctccac ccaggcatcg ccgttgccgg ccttggtgat ggcgtagggg acgagaccct
     1681 tgtccttctg ggtcatcggg tcggcgtagg ttcggccgat cagacgcttg atggcaaaga
     1741 acgtgcgcga aggattggta accgcctggc gcttggccgg ctggccgacc aggcgctcgc
     1801 cctcgtcggt gaaggcgacg atcgacggcg tggtgcgcgc gccttccgaa ttctcgatga
     1861 ccttgggcgt cgtgccttcc atgaccgcga cgcaggagtt ggtggtgccg aggtcgatgc
     1921 cgatgacctt acccatggga tgatcccttc ttcaaagcgg aaacccgaac cggacccatg
     1981 tggcgtccgg cgtgtggaga ggcgacccgg catgggcgtc ggcggtcttt tcggaaagag
     2041 gaccgccgga agcccgatcc ggcgcgttgg ccgcgatata gggacgcgga ttttcaggcg
     2101 caaggggcga agcgaccgcg gaacaatgat cgcgtcgcgc tgaggggctc cgcgctggtc
     2161 tgcggcgcgg ctccgccgct tgcgtcaggc caggctgggc aggttcaacc catgcgtacg
     2221 ggcgcaatcg acggcaatgt cgtagcccgc atcggcatgg cgcatgacgc cggtcgcggg
     2281 atcgttccaa agcacgcgct cgatgcgctt cgaagcttcc ggcgtgccgt cgcagacgat
     2341 gaccatgccg gaatgctggg aaaagcccat gccgacgccg ccgccgtggt gcagcgacac
     2401 ccaggtcgcg ccggacgcgc agttcagcag cgcgttcagc agcggccagt ccgacacggc
     2461 gtcggagccg tcgcgcatcg cctccgtctc gcgatttggt gaagccaccg aaccggaatc
     2521 gaggtgatcg cggccgatga cgatcggggc cttgagttcg cccttcgcca ccatctcgtt
     2581 gaaggcgaga ccgagccggt gccggtcgcc gaggccgacc cagcatatgc gcgccggcaa
     2641 gccctggaac ttgatgcgct tctgcgccat gtcaagccag ttgtgcaggt gcttgttgtc
     2701 gggcagcagc tccttcacct tggcgtcggt gcggtagatg tcctccggat cgccggacag
     2761 cgccgcccag cggaacggcc ccacaccccg gcagaacagc ggccggatat aggcgggcac
     2821 gaaacctggg aaatcgaagg cgtcggcgat gccctcttcc agcgccattt ggcggatgtt
     2881 gttgccatag tcgaccgtcg gaacgcctcg gtggtggaaa tcgagcatgg cgcgcacgtg
     2941 ttcggccatg gacttcttcg ccgctgccgt aacgcccttg ggatcgctgg tccgccgctc
     3001 ttcccagtcc gccagcgtcc agcccttcgg taggtaacca ttgaccgggt catgtgccga
     3061 ggtctggtcg gtgacgcagt ccggcctgac gccgcgccgc accagctccg ggaaaacatc
     3121 ggcggcgttg ccaagcagcg cgacggagag cgccttcttt tccttcgttg ccttggcgat
     3181 gatggcgagc gcgtcgtcga ggtcctttgc cagcacgtcg acatagcccg tgcgcaggcg
     3241 gaactcgatt gacgacggtc ggcattcgac cgcaaggcag caggccccgg ccatcacggc
     3301 ggcgagcggc tgcgcgccgc ccatgccgcc aaggccggcg gtgaggatcc agcggccgga
     3361 aaggtcgccg ttgtagtggc ggcggcccat ttccacaaag gtttcgtagg taccctgaac
     3421 gatgccctgc gagccgatat agatccagga gccggccgtc atctggccgt acatcatcaa
     3481 ccccctgcga tccaattcgt tgaagtgctc ccaggtggcc cagcgcggca cgagattgga
     3541 attggcgatg agcacgcgcg gcgcgtccgc atgggtgcgg aacacgccga ccggcttgcc
     3601 ggactggaca agcaaggtct cgtccgcctc cagcctgcgc agggctgctg ctatgcgatc
     3661 gaagctctcc cagtcgcgtg cggcgcggcc gatgccaccg tagaccacga gctcgcccgg
     3721 cttttccgcg acatccggat cgaggttgtt cattagcatg cgcagcggtg cttccgtcag
     3781 ccagctcttg gcggagatgt cgggtccgcg cggagcacgg atgacgcggg cgttgtcgat
     3841 gcgggtcatg gggtcagtcc ttcgtctcgg cgaacaggag gcaggcggaa aggatgcggg
     3901 cgaggtggcc gcgcatcggc gcagcgaagg cgggatcgta gggcgcgggc caatcggctg
     3961 ccccgaccgg gccgggcgtc tcatgcatgt agccgcggca ggcgagttcc atctgcacgg
     4021 catgcacgcc ccgctccggc gcgccatagc agcgggtgat gtagccgccc ttgaagcggc
     4081 cgttcacgac atggtcaagg ccggtttcgg cacatatccg ggcgatgacc gtttgcagcg
     4141 tttcggcgca ggccgccccg ctgttggtgc cgatgttgaa ctgcggcagg tcgccctcga
     4201 acaggcgcgg aatgcgcgag cggatggaat ggcaatcgta gagcacgagc cgctcatgcc
     4261 gggtccgaag ccgcgcgatc tcgcccgcca gcaccgcatg gtagggatcg aaccaccgcg
     4321 cgcgccgcgc ggcgatctcc gccccatccg gggcgtggcc gtcgcgatac agcgactcac
     4381 catcgaaggt cgtgctgggg caaagttctg tcgttgcctg gcccgggtag agcgaaacac
     4441 cggagggatc gcggttgacg tcgatgaccg tgcgcgacac cgccgtgtga acaaccgtcg
     4501 cgtctagatc ggcagcgaag tcatagagcc ggtcgatcca ccagtcggcg tccttgcgcg
     4561 cgagccacgg cgacaccagc cgcgcctcga tatccggcgg gatgctggtt ccggtgtgtg
     4621 gaaggctgac aatcagcggc gcctgcccgc gccgcacggt gagccagtcg gggatcgcgc
     4681 tcatgcatcc gctcccagtt tcgggaagat atcctcgccg gccgtgcgga ccagatcgcc
     4741 gcgacgcacc agcgccagcg cggcttcgat atccgggtgg aaatgacggt catcatcgag
     4801 gtgatcgaca tgggcgcgca gcaccatgcg caccgcttcc agccggtggc tggagcgcag
     4861 gggcgcgtga aagtcgcagg cctgcgccgc cgccagcagc tcgatcgcca cgatggccct
     4921 ggcattttcc accattcgtg ccaaccggcg cgcgccatgc gccgccatgg agacatggtc
     4981 ctcctgattg gcggaggtcg gaatggaatc gacgctcgcc gggaaggcca tctgccgatt
     5041 ctcggcgacc agcgccgccg ccgtgacctg cggaatcatg aagccggaat tcagacccgg
     5101 cttcggcgtc aggaaggccg gaagcccaga gagcgccgga tcgaccagca tggcgatgcg
     5161 ccgttccgac agtgaaccga tctcgcagac cgccatggcg atcatgtcgg cggcaaaggc
     5221 caccggctcg gcgtggaaat tacccccgga cagggcttcg ccggtgtctg acaggatcag
     5281 cggattgtcg gaaacgccat tcgcctcggt ggcgagtgtt tcggcggcct gccgcagcag
     5341 gtcgagcacg gcgcccatta cctgcggctg gcagcgcagg cagtacggat cctgcacacg
     5401 ctcgtcaccg acaagatgcg aggcgcggat ggcggagccg gccatgaggg cgcgcagcgt
     5461 cgcggccgtc tccatctggc cgcgatggcg gcgcaacaga tggatacgcg gatcaaacgg
     5521 cgcatcggac ccccgcgccg cgtcggtgga cagggcgccg gccaccagcg cagtgcggaa
     5581 caggtcctcg gcggcgaaaa ggcccgcgag cgcgttggcg gtcgaaaact gcgtgccgtt
     5641 gagcagcgca aggccttcct tcgggccaag ctcaaggggc ctc
//
LOCUS       DAOMXN010000175         5651 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_7572_length_5651_cov_8.830593, whole genome shotgun sequence.
ACCESSION   DAOMXN010000175 DAOMXN010000000
VERSION     DAOMXN010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 5651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5651
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7572_length_5651_cov_8.830593"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(333..713)
                     /locus_tag="PK812_11105"
     CDS             complement(333..713)
                     /locus_tag="PK812_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017483717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HRJ70146.1"
                     /translation="MEAFLQRIADAAGIDTETARKAVGIIFAFLKKEAPAESEALLNA
                     TPGAAELAAEHGGGGGGMMGGLMGMMPGGGIMALGGQLMGAGLSMGQIQTVSKELFAI
                     GRETAGEDAMGAIVGAVPGLSQFV"
     gene            869..2395
                     /locus_tag="PK812_11110"
     CDS             869..2395
                     /locus_tag="PK812_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015952612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HRJ70147.1"
                     /translation="MANTIQIPDGTILIGKSSKPEVLTLKLANRHGLITGATGTGKTV
                     TLQTLAEAFAEEGVPVFAADIKGDLSGIAAEGRGEPHFVKRCQEMGLDYHAQKFSVVF
                     WDLFGEQGHPIRTTVSEMGPLLLSRLLQLNEVQEGVLNILFKYADEHKLLLLDLKDLR
                     AMLAEFGSNKELQQELQATYGNISPQSVGAIQRQLLVLENQGGAKFFGEPALDIRDFL
                     KTDRDRRGVINILAADKLMNSPQLYATFLLWLLSELFQELPEVGDLEKPKLVFFFDEA
                     HLLFTDAPKALLTSIEQVVRLIRSKGVGVYFVTQNPLDVPETVLAQLGNRVQHALRAF
                     TPRDQKAVKAAAETFRQNPKFRTDEAITQLAVGEALVSMLEGKGTPSMVERTYIKPPS
                     ARVGPVTPEERQAVINKSPMKGAYDETIDNESAFELLKKRAEAAAGPTQGGVIGTIND
                     MLGGVLGGGTTAKGKKAPPSIIEKAATSAMRSAASSVGRQVANAIVRGVLGSLLKGGR
                     "
     gene            2463..2705
                     /locus_tag="PK812_11115"
     CDS             2463..2705
                     /locus_tag="PK812_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70148.1"
                     /translation="MLILLDRIPVWMVLFAIVSLGLAPFTPEPHIVEKLKMLAAGTLT
                     RPIDVFDLAFHALPWVVGCAKVMRFVVSRQRQGGNS"
     gene            2854..4236
                     /locus_tag="PK812_11120"
     CDS             2854..4236
                     /locus_tag="PK812_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018260822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRJ70149.1"
                     /translation="MPSPDKVLAAVDSNLQPALDRLFALLRIPSISTDPAYAKHCAEA
                     AEWHAKDLKSLGFKAEVRKTAGHPVVLGHRPKKGAPHVLFYGHYDVQPVDPLNLWHNP
                     PFEPKVVEIEKGRKAIVARGACDDKGQVMTFIEACRAWITATGDLPIGVTVLLEGEEE
                     CGSPSLPAFVKANAKELKADFGLVCDTGMWDAKTPAVTSTLRGMVYEEFVVRAANRDL
                     HSGVYGGAALNPNHVVAEICGSFHGKNGRIRIPGFYKGVKAPSEKQLQQWKSLKLTEK
                     AFLGPVGLKSTIGEDDRMLIEHIQSRPTCDVNGIWGGYAGAGSKTVIPAEAGAKVSFR
                     LVAGQDPDAIRKAFRAYVKDRVPKQASVEFRSYVGSSAVAFDTTSAPFDAARAALREE
                     WGKAAVTIGLGGSIPIMHEFRHVLGLDCVLVGFALEDDRVHSPNEKYDLAAFHKGMRS
                     WARIIGKLGG"
     gene            complement(4315..4659)
                     /locus_tag="PK812_11125"
     CDS             complement(4315..4659)
                     /locus_tag="PK812_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70150.1"
                     /translation="MRAKSIALVLAVCAAAGTAEARPMPGPTVTFGPNCHAISRATGQ
                     SSLFVGTVLGGRTTGHRWGDGTMMDYRTFQGCFVTEAACTAWAARHASRYPGAPAYSR
                     CTPVYVGLRPRG"
     gene            4829..5038
                     /locus_tag="PK812_11130"
     CDS             4829..5038
                     /locus_tag="PK812_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HRJ70151.1"
                     /translation="MTKNVGSVDKILRIVIGLVLIAYAIPLGFAKTGWNWIGWIGVIP
                     LATAFLGNCPLYSILGVSTCPVEKK"
     gene            complement(5168..>5651)
                     /locus_tag="PK812_11135"
     CDS             complement(5168..>5651)
                     /locus_tag="PK812_11135"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HRJ70152.1"
                     /translation="SNALLLAAGGIADGRGLAAALMLGADGVLIGTRLWATREALVAP
                     RHHQAILDTGGDGTLRTGIADIARQLNWPKGFTARIRQNAFTQRWHGREEALASAIAT
                     EGPRYRQAFAEGDPDNTGVWFGEAAGLIHDIPDAATAIERIVAHAAGLLAGGPARVRS
                     "
BASE COUNT          981 a         1819 c         1865 g          986 t
ORIGIN      
        1 gtcttcgtcg tcatggaggg aactcccttg ggacgatggg aaggagtgcc ccggcgccag
       61 gtcaagtccg acgcgccggg cgccgcgccg cccatcacgg cgcttgccgg gggcttatgc
      121 tcattttgag ttgaattgga catgaacgcc ggatgcagga aggcggcagt cgtgcgtagg
      181 gagcagggaa agggcgcatc ggtgtcattc cggggcgctt cgcggaagcg cgcccggaat
      241 ccatctcttt gcgaccgacc ggaacgatgg attccggact tggcttcgcc aatccgaaaa
      301 tgacagcttc ggctgccgac cgccgatcag cctcagacga actggctgag gcccggaacg
      361 gcgccgacga tggcgcccat cgcatcctcg cccgccgttt cgcggccgat ggcgaacagc
      421 tccttggaaa cggtctggat ctggcccatg gagaggccgg cgcccatcag ctggccgccc
      481 agcgccatga tcccgccgcc gggcatcatg cccatgaggc ctcccatcat gccgccgccc
      541 ccgccaccat gctcggcggc gagctccgcc gcgccgggcg tggcgttgag cagggcctcg
      601 gactcggccg gcgcttcctt cttcaggaag gcgaagatga tgccgaccgc cttcctggcg
      661 gtctctgtgt cgatcccggc ggcgtcggcg atgcgctgga ggaatgcttc catggggaca
      721 gtccttttct ggcggaggat tccgatggag cagtgctagc acaccttggc cggccccctg
      781 caatgctatt ggcgctggcg cgacgctttg cctatatagg cggcgcgccg gctttcgcgc
      841 gcctttcttc aggacccgag catcgacgat ggcgaatacg atccagatcc cagacggcac
      901 cattctcatc ggcaagagct cgaagccgga ggtcctgacg ctcaagctcg ccaatcgcca
      961 cgggctgatc acgggtgcca ccggcacggg caagaccgtg acgctgcaga cgctggccga
     1021 ggctttcgcg gaggaaggcg ttcccgtctt cgccgccgac atcaagggcg atctctccgg
     1081 catcgcggcc gagggcaggg gcgagccgca tttcgtcaag cgttgccagg aaatgggcct
     1141 ggactaccac gcgcagaagt tctccgtggt gttctgggac ctcttcggcg agcagggcca
     1201 tccgatccgc accacggttt cggaaatggg gccgctgctg ctctcgcgcc tgctccagct
     1261 caacgaggtg caggaaggcg tcctgaacat cctgttcaaa tacgccgacg agcacaagct
     1321 gctgctgctg gacctgaaag acctgcgcgc catgctggcc gagttcggct ccaacaagga
     1381 attgcagcag gaacttcagg cgacctacgg caacatctcg ccccagtcgg tcggcgcaat
     1441 ccagcgccag ctgctggtgc tggagaacca gggcggcgcg aaattcttcg gcgagccggc
     1501 cctcgacatc cgcgatttcc tgaagaccga ccgcgaccgg cgcggtgtca tcaacatcct
     1561 cgccgccgac aagctgatga actccccgca gctttacgcc accttcctgc tctggctgct
     1621 gtcggaactg ttccaggaac tgccggaggt cggcgatctc gagaaaccga agctcgtctt
     1681 ctttttcgat gaagcgcatc tgctgttcac cgatgcaccc aaggccctgc tcacctcgat
     1741 cgagcaggtg gtgcgcctga tccgctccaa gggcgtcggc gtctatttcg tcacgcagaa
     1801 cccgctcgat gtgccggaga cggtgctggc gcagcttggc aaccgcgtgc agcacgcgct
     1861 ccgcgccttc accccgcgtg accagaaggc ggtgaaggcc gccgccgaga ccttccgcca
     1921 gaacccgaag ttcaggaccg acgaggcgat cacgcagctc gcggttggcg aggcgctggt
     1981 ctcgatgctg gaaggcaagg gcacgccctc gatggtggag cgcacctaca tcaagccgcc
     2041 gtccgcgcgc gtcggaccgg tgacgccgga agagcggcag gccgtcatca acaagagccc
     2101 gatgaagggc gcctatgacg agaccatcga taacgaaagc gccttcgaac tgctgaagaa
     2161 gcgggccgag gccgccgccg gccccaccca gggcggtgtc atcggcacga tcaacgacat
     2221 gctgggcggc gtgctcggcg gcggaaccac ggccaagggc aagaaagccc cgccttcgat
     2281 catcgagaag gccgcgactt ccgccatgcg ctccgccgcg tcctcggtgg gccggcaggt
     2341 ggccaatgcc atcgtgcgcg gcgtgctggg cagcttgctg aagggcggga ggtaggcgcg
     2401 aacatgatct tgggcgccgc catgcaggct gtcgttccag ctcgcgtgag gggattgctc
     2461 tggtgttgat cctgctcgat cgaattccgg tctggatggt gctgttcgcc atcgtatccc
     2521 tcggtcttgc tcctttcacg cccgagccgc acattgtcga aaagctgaag atgctcgctg
     2581 ccggaactct cacgcggcca atcgatgtat tcgacctggc gtttcacgcc ctgccctggg
     2641 tcgtcggctg cgccaaggtc atgcggttcg tcgtttcccg gcagcgtcag ggcggcaact
     2701 cgtaaggatc agcggccttt gcgtactcct gcccggggca gaggcgctgg acgtgagcct
     2761 cgaccgggta cagtcaggat gtgatcgcga gtgggttgcg cctgttgccc aatggcttag
     2821 gctccacgga atattgccct cccggagctt tccatgccgt cgccagacaa agttctcgcc
     2881 gccgtcgact ccaacctcca gcccgcgctc gaccggctgt tcgcgctctt gcgcattccc
     2941 tcgatctcga ccgatccggc ctatgcgaaa cactgtgccg aggcggcgga gtggcatgcg
     3001 aaggatttga aatctctggg cttcaaggcc gaggtgcgca agacggcagg acatcccgtc
     3061 gtgctggggc atcgcccgaa gaaaggcgcg ccgcatgtgc tgttctacgg ccactatgac
     3121 gtgcagccgg tcgatcccct gaacctctgg cacaacccgc ccttcgaacc gaaggtcgtc
     3181 gagatcgaga agggccggaa agccatcgtc gcgcgcggcg cctgcgacga caagggccag
     3241 gtcatgacct tcatcgaggc ctgccgcgcc tggataacgg cgaccggaga ccttcccatc
     3301 ggcgtcacgg tgctgctgga gggcgaggag gagtgcggtt cgccctcgct gccggccttc
     3361 gtcaaggcga acgcgaagga actgaaggcg gatttcggcc tcgtctgcga caccggcatg
     3421 tgggacgcga agaccccggc ggtcacgtcc accctgcgtg gcatggtcta tgaggaattc
     3481 gtggtgcgcg cggcgaaccg cgacctgcat tccggcgtct atggcggcgc ggcgctgaac
     3541 ccgaaccatg tggtggcgga aatctgcggc tccttccacg gcaagaacgg gcgcatccgc
     3601 attcccgggt tctacaaggg ggtgaaggcg ccctccgaga aacagttgca gcaatggaag
     3661 agcctcaagc tgacagagaa ggcgtttctc ggccccgtcg gcctgaaaag caccatcggc
     3721 gaagacgacc gcatgctgat agagcacatc cagtcgcgcc cgacctgcga cgtcaacggt
     3781 atctggggcg gttatgccgg cgcgggatcg aagacggtga tcccggccga agccggggcc
     3841 aaagtctcgt tccgcctcgt cgccgggcag gacccggatg cgatccgcaa ggcgttccgc
     3901 gcctatgtga aggatcgcgt gccaaagcag gcgagcgtcg agttcaggag ctatgtcggc
     3961 tcgtcggcag tggcattcga tacgacatca gcgcccttcg atgcggcgcg ggccgccttg
     4021 agggaggaat ggggcaaggc cgcggtcacc atcggccttg gcggctcgat cccgatcatg
     4081 catgagttca ggcatgtgct gggactcgac tgcgtgctgg tcggcttcgc gctggaagat
     4141 gaccgcgtgc attcgccgaa cgagaaatat gaccttgcgg cgtttcacaa gggcatgcgc
     4201 agctgggcgc ggatcatcgg gaagctcggc ggctgactcg ccgcgttcgt tcgcgaacgg
     4261 cgcaacgtcc gccatgatcg ttcatggagg cccagtcgcc gactggcggg cgtctcagcc
     4321 cctcggccgc aacccgacat agacaggcgt gcaccgcgag taggccgggg cgccggggta
     4381 gcggctggcg tgcctggcgg cccaggcggt acaggccgct tccgtgacga agcacccctg
     4441 gaaggtgcgg tagtccatca tggtgccgtc accccagcga tgtccggtcg tgcgcccgcc
     4501 cagcaccgtc cccacgaaca ggctggactg gcccgtcgct cggctgatgg cgtggcagtt
     4561 cggtccgaag gtcacggtcg gccccggcat tgggcgcgcc tcggcggtgc cagcggcggc
     4621 gcagacggcg agcaccagag caatcgattt cgcgcgcatg ttcgaatcat ttcggtggcg
     4681 aggaactcgt tttctactag gctttcgtgt gcaaagttga aagcggtcgc ttgcgcctct
     4741 ggtgccgcgg gtatgatcga tatcaatagc gtcggcgctc cgttccttta tataaggata
     4801 tgcgaatatt ctgaaacagg agggtgcaat gaccaagaat gtgggttcgg tggacaagat
     4861 tctgcgcatc gtcatcggtc tggtgctgat cgcctatgcc atcccgttgg gctttgccaa
     4921 gaccggctgg aactggatcg gctggatcgg cgtgatcccg ctggcgacag ccttcctggg
     4981 caattgcccg ctctattcca tcctcggcgt ttccacctgt ccggtggaaa agaagtaggc
     5041 gcttgtcctg cggccgacga tggcactccg agtgcgcgag gcgcgatccc ggagttgatc
     5101 taagcctatg atatgaaata aaaaagatgg catcaggcgc acgccccgcg cgctccgcat
     5161 tgacccatca ggatcgcacc ctcgccggtc cgccggccaa caggccggcg gcgtgcgcca
     5221 cgatgcgctc gatcgccgtg gcggcgtcgg gaatgtcgtg gatgaggccg gccgcctcgc
     5281 cgaaccagac gccggtgttg tccggatcgc cctcggcgaa ggcctgccgg tagcgcgggc
     5341 cttcggtggc aatcgccgaa gccagagcct cctcgcgccc atgccagcgc tgcgtgaagg
     5401 cgttctgccg gatgcgtgcg gtaaaccctt tcggccagtt cagctgccgg gcgatgtcgg
     5461 cgatgccggt ccgcagcgta ccatcgccgc cggtatcgag gatcgcctgg tgatgccggg
     5521 gggcgaccag cgcctcgcgc gttgcccaaa gccgcgtgcc gatgagaacg ccatcggcgc
     5581 cgagcatgag ggctgcggcg agtccgcgcc cgtcagcgat gccgccagcc gccagcagca
     5641 gcgcgttcga g
//
LOCUS       DAOMXN010000176         5606 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_7671_length_5606_cov_12.416502, whole genome shotgun sequence.
ACCESSION   DAOMXN010000176 DAOMXN010000000
VERSION     DAOMXN010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 5606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5606
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7671_length_5606_cov_12.416502"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1545)
                     /locus_tag="PK812_11140"
     CDS             complement(<1..1545)
                     /locus_tag="PK812_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRJ70153.1"
                     /translation="MGEKKNVLFIMCDQLRYDYLSCYGHPHLETPHIDSLARRGVRFT
                     RAYVQSPVCGPSRMSFYTGRYMRSHGSNWNRFPLRVGEPTLGDALKDVGVRNVLVGKT
                     HMAADVEGMKRLGIDPKSSLGVQVSECGFEPYERDDGLHPDPAPRPLYDRYLKDKGYD
                     RPNPWERHANSGTDEDGAFRNGWLLANSGVAADVAEEDSETPYMTRRAMAFIREAQND
                     PRPWCLHLSYIKPHWPYVAPAPYNAMYGRQHVLRAVRSEAEREDPHPVYAAFMEQRVG
                     RNFSRDDVREAVIPAYMGLIKQIDDQLGRLFAFLEDQGLSDTTMIVFTSDHGDYLGDH
                     WLGEKDLFHDCSARVPLLIFDPSADADATRGTVCDRLVEAIDLAPTFLEYFGGKPRSN
                     VMEGRSLLPLLHGRPPEDWRTYAFSEYDYSMLDARRIAGRGVNECRLFMVFDGRFKLI
                     HSPGLRPMLYDLQADPEELTDLGADPALETERARLMQALTDWALKDHNRITMSDEAIA
                     AYSAARQ"
     gene            complement(1554..3386)
                     /locus_tag="PK812_11145"
     CDS             complement(1554..3386)
                     /locus_tag="PK812_11145"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HRJ70154.1"
                     /translation="MSFFAGPTLEERLVAAGNRPAGFDYLRLVLSTLVIVTHSINVSY
                     GLVYTLNFWTAPIRPFAAAILLMFFALSGFLVAASLERCRTVGGFLALRVLRIVPALA
                     VETTLAALLIGVAFTTLPLAQYFSDPQFARYFLNIVGNVNFFLPGVFADTPWPRYVNS
                     QLWTLPYELYCYVALAALALFGLVRRDARFVIVLVIVNIGIFVVYWWIKKSKMGVTVS
                     GPVLVLGFLYGLALYVFRDRVRWNLPLFALAALATYACLRFEIGDYLAPLPAAYVTVY
                     LGLLQPRRVWIVSSGDYSYGLFLYGFPIQQAVVATMGPAGQHWWINTVIAWPITFVLA
                     FLSWRFVESRLLHFKPAITRAESAWLEGNGAGVTSGQGVSTLWARYRALPYLARIGLP
                     AGLMFLIGSGILALVKRPQEDFPLLARLGVINAHIAEATPGYILVAGDSNAEMINPAI
                     RMCGRELVNAGISGSKIKDFVVRWRGLNFGNPPAAVLISIGTNDLHRKRNPLQAKYLA
                     EFEKQAEILARQAMAASKAVFVMAVPPAERSMTAYEPGAVVAFSERLKGVCERLGCSF
                     IDPFADARDGASGLAKAGGTRDGIHATSYRKAVTVMEPAVCGKL"
     gene            complement(3458..4108)
                     /locus_tag="PK812_11150"
     CDS             complement(3458..4108)
                     /locus_tag="PK812_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70155.1"
                     /translation="MWQAIIGPKPVWYWIVTALVGLAALGMYLVNDRERKDIRWSIAG
                     LPSGAGASGTGYGEPELWVPPRYDAAQLNAFKKKAGDAPLRSYTLGRAGQKSALQHYV
                     RPVLIYNDISYAVLLGGFAFLVNLGLAGLWPRPMAGIAALWALCALVYAIADIREDQA
                     LASMFAQPSAITEEQAATARNWSSIKYWAALASVSGGLSHLAFGVVGSVVNGPDPK"
     gene            complement(4220..4873)
                     /locus_tag="PK812_11155"
     CDS             complement(4220..4873)
                     /locus_tag="PK812_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRJ70156.1"
                     /translation="MLTIYGVYRSRAARNIWLANELGVPFKHVPVIQVNRLPNPNAAD
                     APLHTKSPAFLKVNPNGHVPCIDDNGLVLCESMAINLYLARKHGGPLAPANIAEEGLM
                     TQWSFWAACEMDPLVIQSLYNRVMKPEAERDLKAAEMAEAALKVPLKVLNRYVGASDY
                     LVGGRFTVADLNVSEVVRTMTVSAPQLLDDVPNVKAWLATCHARPHFKAMMEIRNAE"
     gene            4988..>5606
                     /locus_tag="PK812_11160"
     CDS             4988..>5606
                     /locus_tag="PK812_11160"
                     /inference="COORDINATES: protein motif:HMM:NF015549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HRJ70157.1"
                     /translation="MSSPVLLTFAGMRRGMIRSVPLLPGIFVYGAAFGMLAREANLEI
                     WQAAAMSLVVFSGSAQLATLLLLTTGAGLLMIMATIMIVNARYLLYGAALRPWLGGLP
                     ARKAYPTLFFMGDANWVMAMQANADGENDAGYLFGSGLIMAAPWLLGTIAGGALGVLI
                     TNPAVLGLDFFLIAFCTALGAGMAKGRGDLVIMAAAGVTALAADRL"
BASE COUNT         1043 a         1837 c         1789 g          937 t
ORIGIN      
        1 ttgcctggcg gcggaatagg ccgcgatggc ctcgtcgctc atggtgatcc ggttgtggtc
       61 cttcagcgcc cagtcggtca gcgcctgcat caggcgcgcc cgctcggtct ccagcgccgg
      121 gtcggcgccg aggtcggtca gttcttccgg atcggcctgg aggtcgtaga gcatcggccg
      181 caggcccggc gaatggatga gcttgaaacg cccatcgaag accatgaaca gccggcactc
      241 gttgacgccc cgtccggcga tgcggcgggc atcgagcatt gagtaatcgt attcggagaa
      301 ggcgtaggtg cgccagtctt cgggggggcg gccgtgcagg agcggcagca gcgagcgccc
      361 ttccatgacg ttcgacctgg gcttgccgcc gaaatactcc aggaatgtcg gagcgaggtc
      421 gatcgcctcg accagccggt cgcaaaccgt gccgcgcgtc gcatcggcat cggcggaagg
      481 gtcgaagatc agcagcggca ccctggccga gcaatcgtgg aaaaggtcct tctcgcccag
      541 ccagtggtcg ccgaggtaat cgccatgatc cgacgtgaag acgatcatgg tggtgtcgga
      601 cagcccctgg tcctcaagga aggcaaacag tcggccgagc tgatcgtcga tctgcttgat
      661 cagccccatg taggccggaa tcaccgcctc gcgcacgtca tcccgcgaga aattgcgccc
      721 gacccgctgc tccatgaagg cggcgtagac cggatgcggg tcctcccgct ccgcctcgct
      781 gcgcacggcg cgcagcacgt gctggcgacc atacatcgcg ttgtagggcg caggcgcgac
      841 atagggccag tgcggcttga tgtaggacag atgcaggcac caggggcgcg gatcgttctg
      901 ggcctcgcgg atgaacgcca ttgcgcggcg cgtcatgtac ggcgtttcgg aatcctcttc
      961 cgcgacgtcg gcggccactc cggaattggc gagcagccag ccgttgcgga acgcaccatc
     1021 ctcgtccgtg ccggaattgg cgtgacgctc ccaggggttc ggccggtcgt agcccttgtc
     1081 cttcagatac cggtcgtaga gcgggcgcgg cgccggatcg ggatgcaggc cgtcatcccg
     1141 ttcatacggt tcgaaaccgc actccgaaac ctgcacgcca aggctcgatt tcggatcgat
     1201 cccaagcctc ttcatgccct cgacatcggc cgccatgtgc gtcttgccga ccagcacgtt
     1261 gcgaacgccg acgtccttga gcgcatcgcc gagggtcggt tcgccgacgc gcagcggaaa
     1321 ccggttccag ttggaaccgt gcgagcgcat gtagcggccg gtgtagaagc tcatgcgcga
     1381 cggcccgcag accggcgact gcacataggc gcgcgtgaaa cggacgccgc gccgcgccag
     1441 cgaatcgatg tgcggcgtct caaggtgcgg atggccatag cagctcaggt agtcgtaccg
     1501 gagctggtcg cacatgataa acaggacgtt cttcttctct cccacgactg tcctcacagc
     1561 ttgccgcaga ccgccggttc catgacggtg accgccttgc gatacgacgt agcatgaatg
     1621 ccgtcacgcg tgcccccggc cttggccagg ccgctggcgc catcgcgggc atcggcgaag
     1681 gggtcgatga agctgcatcc gagcctctcg cacacgccct tgagccgttc ggaaaaggcg
     1741 accaccgcgc ccggctcgta ggccgtcatc gaccgttcgg ccggcggcac cgccatgacg
     1801 aacaccgcct tgctggcggc catcgcctgc cgcgccagga tttccgcctg cttttcaaac
     1861 tcggcgagat atttggcctg caggggattg cgcttgcgat gcagatcgtt ggtgccgatg
     1921 gaaatgagga cggcggctgg cggattgccg aagttcaagc cacgccagcg tacgacgaaa
     1981 tccttgatct tcgacccgga aatgccggcg ttgaccagtt cacgcccaca catgcgtatc
     2041 gccgggttga tcatctccgc gttggaatcg cccgcaacga ggatgtagcc gggcgttgcc
     2101 tcggcgatgt gggcgttgat gacgccgagg cgcgccaaca gcgggaagtc ctcctgaggg
     2161 cgcttgacca gagccaggat gcccgagccg atcaaaaaca tcagccccgc cggcaagccg
     2221 atgcgcgcca gatacggcaa cgcgcgatac cgcgcccaga gcgtcgacac tccctgccct
     2281 gacgtcaccc cggcaccatt cccttcgagc cacgcgcttt cggcccgagt gatggccggt
     2341 ttgaaatgca agagccggct ttccacaaag cgccacgaaa ggaaagccag gacgaaggta
     2401 atcggccagg caatcaccgt gttgatccac cagtgctggc cggctggccc catggtggcg
     2461 acaaccgcct gctggatcgg gaatccgtag aggaacaggc cgtaggaata atccccggac
     2521 gagacgatcc acacacgccg gggctggagg agaccgaggt agaccgtcac ataggcggcg
     2581 gggagcggcg cgagatagtc gccgatctcg aacctgaggc aagcataggt cgccagcgcc
     2641 gccagcgcga acagcggcag gttccagcgc acacgatcgc ggaacacgta gagtgccagc
     2701 ccatagagga acccgagcac cagaacaggg ccggaaaccg tgacgcccat cttcgacttc
     2761 ttgatccacc agtagacgac gaagatgccg atgttgacga tcaccagcac gatcacgaac
     2821 cgtgcatcgc gccgaaccag gccaaacagc gccagcgccg caagggctac atagcaatag
     2881 agctcgtagg gaagcgtcca gagctggctg ttgacgtagc gcggccaggg cgtgtcggcg
     2941 aagacgccgg gcaggaagaa gttcacgttg ccgacgatgt tcaggaagta gcgcgcgaac
     3001 tgcggatcgg aaaaatactg cgccaacggt agagtggtga aggccacgcc gatcagcagc
     3061 gccgccagcg tcgtttcaac cgccagcgcc ggcacgatgc gcagcacgcg cagcgccagg
     3121 aagcccccca cggtgcggca ccgttccagg ctcgccgcca cgagaaagcc ggagagcgcg
     3181 aagaacatca gcaggatcgc tgcggcgaac ggccggatcg gcgccgtcca gaaattcagc
     3241 gtgtagacga ggccgtagct gacgttgatg ctgtgcgtga cgatgacgag ggtggacagc
     3301 accagacgca gataatcaaa gcccgccggc cggtttcccg ctgcgacaag ccgctcttcc
     3361 agcgtcggtc cggcgaaaaa cgacaccctg tccagtcccc cgatcgatcc caggcgcgcc
     3421 gcttatgcgt caggggagca ggtgaggcaa gccggcgcta cttcggatcg gggccattca
     3481 cgacggaccc gaccaccccg aaggccagat ggctcagccc cccggacacg gaagcgaggg
     3541 ccgcccagta cttgatcgaa ctccagtttc gcgccgttgc ggcctgctct tcggttatgg
     3601 cggagggctg cgcgaacatc gaggccagcg cctgatcttc ccggatgtcg gcgatcgcgt
     3661 agaccagcgc gcacagggcc cacagcgcgg cgatcccggc catgggcctt ggccaaaggc
     3721 cggcaaggcc aagattgacc aggaaagcga agccgcccag cagcacggca taagagatgt
     3781 cgttgtagat gaggaccggc cggacataat gctgaagcgc cgatttctgt ccggcccgcc
     3841 ccagcgtgta ggaccgcagc ggcgcgtccc ccgccttctt cttgaacgcg ttcaactgcg
     3901 ccgcatcgta ccgcggcggc acccaaagct ccggctcgcc ataccccgtg ccactggccc
     3961 cggcacctga aggcagaccg gcgattgacc agcggatatc cttccgctcg cgatcattga
     4021 cgagatacat gccaagggcc gccagaccga ccagcgccgt gacgatccag taccaaaccg
     4081 gtttgggtcc gatgatcgcc tgccacatcg gttgcctccc gagcgaacga accggcggac
     4141 aagccgccgt cccgactgtc tcagatgcgg ctgacgaagc ctaggggccc caccgtgaat
     4201 tcgacgcaag cagcgtgacc tactccgcgt ttctgatctc catcatcgcc ttgaaatgcg
     4261 ggcgcgcgtg gcaggtcgcg agccatgcct tcacgttcgg cacgtcatcg agcaactgcg
     4321 gcgcgctgac ggtcatggtg cggaccactt ccgagacgtt gaggtcggcc accgtgaagc
     4381 gcccgccgac cagatagtcc gacgcgccga catagcggtt cagcaccttg agcggcacct
     4441 tcagcgcggc ttccgccatt tccgccgcct tgagatcgcg ttcggcctcc ggcttcatca
     4501 cgcggttgta gaggctctgg atgaccagcg gatccatctc gcaggcggcc cagaaggacc
     4561 actgggtcat caacccttcc tcggcgatat tcgctggcgc cagcgggcca ccatgctttc
     4621 gtgccagata caggttgatc gccatggatt cgcacagcac caacccgttg tcgtcgatgc
     4681 agggcacgtg accgttcggg ttcaccttga ggaacgccgg cgacttcgta tgcagcggcg
     4741 catccgcggc gttcggattc ggcagccggt tcacctggat gaccggcaca tgcttgaacg
     4801 gaacgccgag ttcgttggcg agccagatgt tgcgcgccgc gcgcgaccgg tagacgccgt
     4861 agatggtcaa catggtgctt taattcccct catcagaagc gcgggccgtc caacctgcct
     4921 ccgcgctgga ttgactcgga ccgcataatc ggattgccct tcaatcttag caatcaaaga
     4981 tttcttcgtg tcctcgcctg tcctcctcac cttcgccggc atgcgccgcg gcatgatccg
     5041 ctcggtgccg ctcttgcccg gcatcttcgt ctatggcgcc gccttcggca tgctggcgcg
     5101 cgaggccaat cttgagatct ggcaagctgc cgccatgagc ctcgttgtgt tttccggctc
     5161 cgcccagttg gcaacgctgc tcctgctgac gaccggcgcg gggctgctga tgatcatggc
     5221 gacgatcatg atcgtcaacg cgcgttacct gctgtacggc gcggccctgc gcccgtggct
     5281 tggcggattg ccggcgcgga aggcctaccc cacgctgttc ttcatgggcg atgccaactg
     5341 ggtcatggcc atgcaggcca atgcggatgg cgagaacgat gccgggtatc tgttcggatc
     5401 cggactgatc atggcagcgc cctggttgct cggcaccatc gctggcggcg cgctgggtgt
     5461 gctgatcacc aatcccgccg tgcttggcct ggacttcttc ctgatcgcct tctgcacggc
     5521 gctgggggcg ggcatggcca agggccgcgg tgacctcgtc atcatggcgg cggcgggcgt
     5581 gaccgcgctg gcggccgatc gcctgc
//
LOCUS       DAOMXN010000177         5407 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_8036_length_5407_cov_9.760277, whole genome shotgun sequence.
ACCESSION   DAOMXN010000177 DAOMXN010000000
VERSION     DAOMXN010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 5407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5407
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8036_length_5407_cov_9.760277"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /locus_tag="PK812_11165"
     CDS             <1..1176
                     /locus_tag="PK812_11165"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HRJ70158.1"
                     /translation="AAGLAAASRLSDALEGAKITLIDARKPHYYQPGFTLIASGIVPP
                     SYAVSATRDYVARGVELIEDTVAEFDPDGNAVVTGAGKRISYDFLIVATGLRLDYGAI
                     AGMDAALIGKDGIGSIYAGPEAAAATWREMSAFADRGGVGLFLRPNTEMKCAGAPLKY
                     TFITDDTLRRRGNRGKAELIYAANNKALFSVPIVAEKVRMLYQERGVKTVYDHVLSAI
                     DPGRKVATFKTPAGDQQVKYDFINVVPPMRAPDAVRKSPLAWREGPWAADGWMEVDMK
                     TLRHKRYPNVFGIGDIAGVPKGKTAASVKWQTPVAVSHIVAAIGKTTSTETYNGYTSC
                     PLITRIGQAMLIEFDYENNLVPSFPGVIAPLEELWISWVMKKVALKPTYIAMLRGQA"
     gene            1187..1540
                     /locus_tag="PK812_11170"
     CDS             1187..1540
                     /locus_tag="PK812_11170"
                     /inference="COORDINATES: protein motif:HMM:NF036440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5368 domain-containing protein"
                     /protein_id="HRJ70159.1"
                     /translation="MKDLDPAVFLAVFQEILGPLFWPLVAVIVLGALGLLFVLVRERG
                     IVARRFVWSQVVGLAGGAFGIWFMLWLTNSRMSDGGGPVDWLLSVIIWVVGAAGAAIG
                     AYVVLGLITPRRTVD"
     gene            1562..2812
                     /locus_tag="PK812_11175"
     CDS             1562..2812
                     /locus_tag="PK812_11175"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRJ70160.1"
                     /translation="MTAIFPDMVPAIAPERLPQLIDIVRWIVAAGNVGRSERGLTEGL
                     ANLLVEAGIPVHRISMGSNVLDPVLQSRSYVWTAETGTEPMRFERGLRQSENWLKSTL
                     YWMMSNEQSRLRVPLDSARANDFPLLVSFRERGYTDYYARLEAYGERAQASGARGLMA
                     TFTSRDPAGFSDEHLAFLDAVLPAFALAFLARLNTRILARALSTYLGKTPAERVLKGE
                     ITRGQGQTVDAIVWMSDLSGFTKTADALPREDILEFLNAHAEIVSDAIEAHGGEVLKF
                     IGDGILAVFPRANAQSWAASQALDAAIRVQVAAKIMANQRREQDKPSAEIIISLHRGD
                     VLFGNFGSQDRLDFTALGSAVNEASRICSLAKTLDQKLLVSDDFKEVLPPGDDRLVSV
                     GRYALRGVADPKHLYTLDRAVVRV"
     gene            complement(2820..3602)
                     /locus_tag="PK812_11180"
     CDS             complement(2820..3602)
                     /locus_tag="PK812_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043461 - proton-transporting ATP synthase
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP12 family protein"
                     /protein_id="HRJ70161.1"
                     /translation="MRDLLYPEDHAWRGGDEAVDRARASQKRPLPKRFYKDVAVEAGE
                     GGHLVTLDGRKARTPGKSLLKLPTRAAANVVAAEWAAQGEVIDPAEMHATRIANVGID
                     RVEAVRAEVIAEIVRYAGSDLVCYRADGPEGLIERESTAWDPVLRHARDVHGARFVLS
                     QGIGHAEQPEAALAAVEAAFAAVADPVALAALHTLTTLSGSALIVLMLRDGALAAEAA
                     FLASSVEEDWNVHLWGEDGEATLRRARRRQEFLAAAALLKAL"
     gene            complement(3609..4685)
                     /locus_tag="PK812_11185"
                     /pseudo
     CDS             complement(3609..4685)
                     /locus_tag="PK812_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
     gene            complement(4691..5062)
                     /gene="crcB"
                     /locus_tag="PK812_11190"
     CDS             complement(4691..5062)
                     /gene="crcB"
                     /locus_tag="PK812_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020012485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HRJ70162.1"
                     /translation="MKDAALIFLGGGAGSVLRWLTGLLMLRSFGPYFPLGTLAVNVVG
                     CFVMGVLFRVLPVGSGDARLLLMTGLLGGYTTFSAFALDTAALWMRNEPGLAALYVAA
                     SVIASLGGVALGLVLGKWMVG"
     gene            complement(5064..>5407)
                     /locus_tag="PK812_11195"
     CDS             complement(5064..>5407)
                     /locus_tag="PK812_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70163.1"
                     /translation="FAFYFGQLRYRRHLVARPDLPKDRDAALFSSLMETVGRPINQYA
                     FGDVPKLIRSIDAVLAHDLKSPDRVTPKAQFPQAHDSVRAGLQTMKREIAARRAYIRE
                     ERAKNGLPNRS"
BASE COUNT          906 a         1899 c         1704 g          898 t
ORIGIN      
        1 gcggcaggcc tcgccgccgc ctcccgactc tcggacgcgc tggagggcgc gaagatcacg
       61 ctcatcgacg cgcgcaagcc gcactactat cagccgggct tcaccctgat cgcttccggc
      121 atcgtcccgc cgagctatgc ggtatccgcc acgcgggact atgtggcgcg cggcgtggag
      181 ctgatcgagg acaccgtcgc cgagttcgat cctgacggca atgccgtggt cacgggcgct
      241 ggcaagcgca tctcctatga ctttctcatc gtggccactg gcctcaggct cgactacggc
      301 gcgatcgccg gcatggacgc ggcactgatc ggcaaggacg gcatcggctc gatctatgcc
      361 ggcccggaag ccgccgccgc gacctggcgg gagatgtcag cctttgccga caggggaggc
      421 gtcggcctgt ttctgcgccc caacaccgag atgaaatgcg cgggcgcgcc gctgaaatac
      481 accttcatca ccgatgatac cctgcgtcgc cggggcaatc gcggcaaagc ggagctgatc
      541 tacgccgcca acaacaaggc gctgttctcc gtgccgatcg tcgcggagaa ggtgcgcatg
      601 ctctaccagg agcgtggcgt gaagacagtc tacgaccacg tcctgtccgc catcgaccct
      661 ggccgtaagg tggcgacctt caagacgcct gccggtgatc agcaggtgaa gtacgacttc
      721 atcaacgtcg tgccgccgat gcgcgcgccc gacgccgtgc gcaagagccc gctggcctgg
      781 cgcgaaggcc cctgggccgc cgacggctgg atggaggtcg acatgaagac gctgcgccac
      841 aagcgctacc ccaacgtctt cggcatcggc gacattgccg gcgtgccaaa gggcaagacg
      901 gcggccagcg tgaagtggca gacgcctgtc gcggtcagcc acatcgtcgc cgcgatcggc
      961 aagacgacct cgaccgagac ctacaacggc tacacctcct gcccgctgat cacgcgcatc
     1021 ggccaggcca tgctgatcga attcgactac gagaacaatc tcgtgccctc tttccccgga
     1081 gtgatcgcgc cgctggaaga gctttggatt tcctgggtga tgaagaaggt ggcgctcaag
     1141 cccacctaca tcgccatgct gcgcggccag gcctgaggag gacaccatga aagacctcga
     1201 tcccgccgtt ttcctcgctg ttttccagga aatccttggt ccattgttct ggccgctggt
     1261 ggcggtcatc gtgctgggcg cgctgggcct gctgttcgtg ctggtccgcg agcgcggcat
     1321 cgtcgcccgt cgcttcgtct ggtcacaggt cgtcggactt gccggcggcg ccttcggcat
     1381 ctggttcatg ctctggctga cgaattcgcg catgagtgat ggcggcggtc cggtggactg
     1441 gctgctgagc gtgatcatct gggtggtcgg cgcggccgga gcggccatcg gcgcctatgt
     1501 cgtccttggg ctgatcacgc cacgccgcac ggttgactga cgccccttgc aacgccggga
     1561 gttgaccgcc atcttcccgg acatggtccc cgcaatcgcc cccgagcggc ttccgcagct
     1621 tatcgacatc gtccgctgga tcgtcgccgc cggaaacgtc ggccgttccg aacgcggact
     1681 caccgaaggg ctggcgaacc tgctggtcga ggccggcatt ccggtgcatc gcatctcgat
     1741 gggctcgaac gtgctcgatc cggtgctcca gtcgcgctcc tacgtctgga cagctgagac
     1801 cggcacggag ccgatgcgct tcgagcgtgg gctccggcag tcggaaaact ggctgaagag
     1861 cacgctctac tggatgatgt ccaatgagca gagccgcctg cgcgtgccgc tcgattcggc
     1921 gcgcgcgaat gactttcccc ttctcgtcag cttccgcgag cgtggctaca ccgactacta
     1981 cgcgcgactc gaagcctatg gcgagcgcgc gcaggcctcg ggcgcacgcg gcctgatggc
     2041 gacattcact tcccgtgatc cggccggttt ctccgatgag catctggcct ttctggatgc
     2101 cgtgctgccg gccttcgccc tggccttcct tgcccggctc aacacccgca tcctggcgcg
     2161 ggcgctgtcg acctatctcg gcaagacgcc ggccgaacgc gtactgaagg gcgaaataac
     2221 gcgcggccaa ggccagacgg tggacgccat cgtctggatg tcggacctct ccggattcac
     2281 caagaccgcc gacgccctgc cccgcgagga catcctcgaa ttcctcaacg cccatgccga
     2341 gatcgtctcc gatgccatcg aggcccatgg cggtgaagtg ctgaaattca tcggcgacgg
     2401 catcctggcg gtcttcccgc gcgccaacgc gcaaagctgg gcggcctcac aggcccttga
     2461 tgccgccatc cgcgtgcagg tcgccgcgaa gatcatggcc aaccagcggc gtgagcagga
     2521 caagccctcg gcggaaatca tcatctcgct gcatcgcggc gatgtgctgt tcggcaattt
     2581 cggctcgcag gaccggctgg atttcacggc tctgggttcg gccgtgaacg aagccagccg
     2641 catctgctcg ctggccaaga cgctggacca gaagctgctg gtatccgacg atttcaagga
     2701 agtcctgccg cccggcgacg accggctggt ctcggtcggc cgctacgccc tgcgcggcgt
     2761 cgccgacccg aaacacctct acacgctcga tcgcgccgtc gttcgggtgt gaataaaggc
     2821 tacagcgctt tcagcagcgc cgctgccgcg agaaactcct gccgccgccg cgcgcggcgc
     2881 agcgttgcct cgccgtcctc gccccagaga tgcacgttcc agtcctcctc caccgaggag
     2941 gccaagaacg cggcttcggc tgcaagcgcg ccatccctga gcatcagtac gatcagcgcc
     3001 gaaccggaga gcgtggtcag cgtatgcagg gccgccagcg cgacaggatc ggcgacagcg
     3061 gcgaacgccg cctccaccgc cgccaacgcc gcttcgggct gttcggcgtg gccgatgccc
     3121 tgcgacagta cgaaacgtgc gccatgaacg tcccgggcgt ggcgcagcac cggatcccag
     3181 gctgtactct cccgctcgat caacccttcc gggccatccg cgcgatagca aaccagatcg
     3241 gacccggcat agcgcacgat ctcggcgatc acctcagcgc gcaccgcctc cacgcggtcg
     3301 atgccgacat tcgcgatgcg tgtcgcatgc atctcggccg gatcgatcac ctcgccctgc
     3361 gccgcccact ccgccgccac cacgttggct gccgcccgcg tcggcagctt gagcagggac
     3421 ttgcccggcg ttcgtgcctt gcgcccgtcg agcgtgacca gatgcccgcc ctcaccggcc
     3481 tcgacggcga catccttgta gaaccgcttg ggcagcggcc gcttctgcga ggcgcgcgcg
     3541 cggtccacgg cctcatcgcc gccgcgccag gcatggtctt cgggatacag cagatcacgc
     3601 atcgtcgctc acttctccgg cgcgttctcg atgggatcga accgcgtgga gtcgaagccg
     3661 agcaggttga aggtctgctg catatgcggc ggcagcgggg cggtcacgtc gaccggctcg
     3721 ccggtcctgg gatggggaat gacaatgcga cgggcgagca gatgcagccg gttctgcatc
     3781 ccgccgggca attcccagtt ctggatgtcg aaatacttcg gatcgccgac gatcgggtgt
     3841 ccgatatggg cggcatgcgc gcgcagctgg tgcgtgcggc cggtcaccgg cttcatcgac
     3901 agccaagcca gcttctgcgc cgccgtctcc accacggcgt aataggtcag cgcgtgatca
     3961 gccccctcgt caccatgttg cgccaccttc atcctggcat cgccgtgttc gtcctcaccc
     4021 ttggcgagat aggtcgagac ccgcccctgc ttgacgcgcg gcacgcccac caccaccgac
     4081 cagtagatct tgcgcgccga acgcgagcga aacgtcttcg ccagcgtgga cgccgccagg
     4141 cgcgtcctgg cgatgatcag gcagccggcg gtatccttgt cgagccggtg aacgaggcgg
     4201 ggcttctgcc catccttgtc ggtcagcgcc gccagcatgc cgtccacatg gcgcaccgtg
     4261 ccggagccgc cctgcacagc cagccccgcc ggcttgttca gcaccatcat ctcgcggtct
     4321 tcgtacagcg tgatggactt caggaacttc gcgtcgtcgt cgccgcgctt ggccaccttc
     4381 agggattgcg gtcgcacatt gtccggcgca ccggcccgac ctgtgcgccc caccgcctcg
     4441 ccacgcgttg taaccggacc ggcagcggat ggagcctcgc ctgcgccgcc gccgagaggc
     4501 ggaatgcgga tcgcctgccc gggctccagc cgggtgtcgg acttggcccg cttgccatcg
     4561 acgcgcacct gcccggtgcg cagaagcttc tgcagatggc cgaacggcag cgcgacgaaa
     4621 tgcgtcttga accagcgatc gagccgcatc cccgcctcgt cagccttcac cgtgcgggtc
     4681 gtgaccgcgc tcatcccacc atccacttgc caagaaccag gcccagggcg accccgccga
     4741 gcgacgcgat caccgacgcg gccacataca gagccgccag tccgggttcg ttgcgcatcc
     4801 acagcgccgc cgtgtcaagc gcaaaggcgg agaacgtggt atagccgcca agcagtccgg
     4861 tcatcagcag cagccgcgca tctccgctgc cgaccggcag aacacggaac aacaccccca
     4921 tcacgaagca gccgacgaca ttcacggcca gggttccgag cggaaagtac ggcccgaagc
     4981 tgcggagcat caacaggccg gtcagccagc gcaacaccga gccggctccg ccgccgagga
     5041 agatgagcgc cgcgtccttc atgtcagctc cggttcggca ggccgttctt ggcgcgctcc
     5101 tcgcggatgt aggcgcggcg cgcggcgatc tcgcgcttca tggtctgaag gccggcacgg
     5161 accgaatcgt gcgcctgcgg gaactgcgcc ttcggcgtga ccctgtcagg cgacttcaga
     5221 tcatgggcca gcaccgcatc gatgctgcgg atcagcttcg gcacgtcgcc gaaggcatac
     5281 tggttgatgg gacgcccgac cgtctccatc agcgaggaaa agagcgcggc gtcccgatcc
     5341 ttcggcaggt cgggccgcgc caccagatgc cggcggtagc gcaactggcc gaaatagaag
     5401 gcgaaga
//
LOCUS       DAOMXN010000178         5335 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_8180_length_5335_cov_12.795076, whole genome shotgun sequence.
ACCESSION   DAOMXN010000178 DAOMXN010000000
VERSION     DAOMXN010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 5335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5335
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8180_length_5335_cov_12.795076"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1092
                     /locus_tag="PK812_11200"
     CDS             <1..1092
                     /locus_tag="PK812_11200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRJ70164.1"
                     /translation="GQRPGGPGAGRGPGGGAPIPVAVAAVQKKTMPFRVEAVGTAQAI
                     ASVTIRSRVDSQIEQILFQDGAHVTAGDVLARLDSRAIEAQLRQAEANLARNRVNLDL
                     AQRTLKRGLDLANQNFATQQRLDENRANANALEALVRADEAQVEHLKTQFSYYTIRAP
                     ISGKAGLANLKPGNMARQSDGAVALTTINQMSPIYVSFALSQRYFDEVRKSLERGDSS
                     VEAVIQGAGKRATGKLALVENAMDSATGTIGVRAVFENKDEALWPGAITNLKITLRED
                     KDVTVVPREAVQMGQRGTFVFTVVDGMARMRPVQVQRSMDRETIIASGLNGTETVVID
                     GQLQLIEGARVTPRQGASSALPGRPAGNL"
     gene            1115..4384
                     /locus_tag="PK812_11205"
     CDS             1115..4384
                     /locus_tag="PK812_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRJ70165.1"
                     /translation="MTLSELSIRRPVMATLITLSFVIFGVFAYMKLPVAALPRVDFPT
                     IDVSAQLPGASPETMAASVAAPLERQFATISGITSMTSVSRLGATNITIQFDLDRSID
                     GAALDVQSAISAVLRRLPPDLPAPPSFRKVNPADQPILFMVLRSPTLPLSQVHEYAET
                     ILQQQIAQITGVAQVSIFGAQKYAVRIFFDPDALAARGLSPQDIRNAVANANSNLPVG
                     QLRSGPQRITLEATGQMEKARDYANVIVSWRGGGTQAVRLSDVARIEDSVENDQTAAW
                     SNGERSLPLAVFRQSDANTVETVQRIRDKLPALRAQLPASVELIVANDSSLPIKEAVH
                     DVQLTLAFSVVLVILVIFMFLKRLSATVIPTLALPVSLIGTFAFMYLFGYSLDNISLL
                     ALTLAVGFVVDDAIVMLENIVRHVEKGMKPFEAALKGAREIGFTILSITFSLVAVFIP
                     VFFMSGVVGRVFREFAVVISVSILVSGFVSLTLTPMLCARFLKEEDHSKKPGLISRGL
                     DAGFNGLLWLYRVTLDIALKYRLATLALTFLSAWYALHLYRTIPQGFFPLEDTGLIRA
                     STEGPADTSFEAMAERQLKLIEIVKNDPAVSFFNAGVGGFSSVNQGFMFISLKPRAER
                     DSVQQVIGRLRQATSQIPGITFIAAPVQNLNLNGGRSSRSMYQYTLTGPNFQELVDLS
                     PGHVTKLRALPQLRDVSLDLQLRNPQIRMDIDREQAQRMGVSMDQIRQTLYNSFGSRQ
                     ISTIYSQANDFPVIMEADRAFQSSPDALSRLYVRSQNAQVAPAQGTATQGGTSAATQN
                     FAVANIPLDALVKTSSNVGPLAVTRSQQLPAVTISFNTAPGVSLGEAVDAIKRVEREA
                     NLPATVSTRFAGSAQLFEEAKAGQAMLILAAVLVIYILLGILYESFIHPITILSGLPS
                     AGLGALIFLNYYSMDLSVIALIGILMLIGIVKKNAIMMVDFAIERRRDGTDALTAIRE
                     ACLIRFRPIIMTTLAAALGALPIAMGIGAGAELRQPLGIAVVGGLAVSQIMTLYITPV
                     VYFYLDKIDSFFAGRETEGAGAEFAPALTVIETPVVDQDAAQPGAGVAPKTSAAAPAT
                     R"
     gene            4520..>5335
                     /locus_tag="PK812_11210"
     CDS             4520..>5335
                     /locus_tag="PK812_11210"
                     /inference="COORDINATES: protein motif:HMM:NF022515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2865 domain-containing protein"
                     /protein_id="HRJ70166.1"
                     /translation="MSGSDIVRAYRDRSTGAGRRAPGWRKPVIAGAAAASILAFGAII
                     VQANDSAGVYEVNKTYNSARAARPAVLPQIFRSEPRPVVQTALSYAPIFGALAPHQAR
                     GDQKRAVPPGSPRGVSVSPKAKSKDIARGTIDDGTSYCVRTCDGYFFPLGNPDAGDLA
                     AHDESCARACPASDTAVYVAPAGSRGIDDAVNRRGERYETLRTAFSHRMQSDSACRCT
                     GPGKQRNYSLMDDFTLRSGDYVMSTDGMRIYRGEAGRVRGPRDFARVDASKLPA"
BASE COUNT          936 a         1765 c         1665 g          969 t
ORIGIN      
        1 ggtcaacgcc caggcggacc gggagcgggg cgcgggccgg gcggcggcgc tcccattccg
       61 gtggcggtgg cagcggttca gaaaaagacg atgccgttcc gggtcgaggc tgtaggcacg
      121 gcccaagcca tagcttcggt cacgatacgc tcacgggtcg acagccagat tgaacagatc
      181 ctgtttcagg acggcgcgca tgtgacggcg ggcgatgtgc tcgcgcggct cgattcccgc
      241 gcgatagagg cgcagttgcg gcaggccgaa gccaatctag cgcgaaaccg cgtcaatctt
      301 gatctggcgc agcgcacgct gaaacgcggg ctcgaccttg ccaaccagaa tttcgccaca
      361 cagcagcgtc tcgacgagaa ccgcgccaac gccaacgcgc ttgaagcgct ggtgcgcgct
      421 gacgaagcgc aggtggaaca cctcaagacg caattcagct actacacgat acgcgccccg
      481 atcagcggca aggccgggct cgccaatcta aagccaggca acatggcgcg acagagcgac
      541 ggtgccgtcg ccctgacaac cataaaccag atgtcaccga tctatgtcag tttcgcgctg
      601 tcacagcgat atttcgacga agtgcgcaag tcactggagc gaggcgacag ttctgtcgag
      661 gcggtgattc agggcgcggg caagcgagcg accggcaagc tggcgctggt ggagaacgcc
      721 atggacagcg ccaccggcac gatcggtgta cgggctgtct tcgagaacaa ggacgaagcg
      781 ctctggcctg gcgcgatcac gaacctcaag atcacgttgc gcgaagacaa ggacgtgacc
      841 gttgtgccgc gtgaagccgt gcagatgggc cagcgcggca ctttcgtgtt caccgtcgtc
      901 gacggaatgg cgcgcatgcg accggtccag gtgcaacgat ccatggatcg tgaaacgatc
      961 atcgcctcgg ggctgaacgg caccgagact gtcgtgatcg acggacagct gcagctgatc
     1021 gagggggcgc gcgtcacgcc tcgtcagggt gcgtcgtcgg cgttgccggg ccggccggcc
     1081 ggcaacttgt gagaagagct gaagggggga agggatgaca ctttccgagc tttcgatccg
     1141 ccggccggtt atggccactt tgatcacgct ctcgtttgtg atcttcggcg tcttcgccta
     1201 catgaagctg ccggtggccg cgctgcccag ggtcgacttc cccaccatcg acgtttccgc
     1261 gcaattgccc ggcgcgagcc ccgaaaccat ggcggcgtcc gtcgcggcgc cgctggaacg
     1321 ccagttcgcg accatctccg gcatcacctc gatgacctcg gtgtcgcgcc tcggcgccac
     1381 caacatcacc atccagttcg acctggaccg gtccatcgac ggtgccgcgc tcgacgtgca
     1441 gtccgccatc agcgcagtgc tgcgccggtt gccgccggac ctgccggccc cgcccagctt
     1501 ccgcaaggtg aacccggcgg atcagcccat cctgttcatg gtcctgcgct cgccgacgct
     1561 cccgttgtcg caggtgcacg agtatgccga gacgatcctt cagcagcaga tcgcgcagat
     1621 caccggcgtg gcgcaggtca gcattttcgg cgcgcagaaa tatgccgtgc gcatcttctt
     1681 cgatccggac gcgctggctg cccgcggcct gagccctcag gacatccgca acgcggtcgc
     1741 caatgccaac tccaacctgc cggtcggcca actccggtcc ggcccgcaga ggatcacgct
     1801 cgaagcgacg ggccagatgg agaaggcccg ggactacgcc aatgtcatcg tctcctggcg
     1861 cggcggcggc acgcaggcgg tccgcctgtc ggatgtggcg cgcatcgagg attcggtcga
     1921 gaacgaccag acagcggcgt ggagcaatgg cgagcggtcc cttccgctgg cggtgttccg
     1981 gcagtccgat gcgaacacgg tcgagacggt gcagcgcatt cgcgacaaac tgccggccct
     2041 gcgcgcgcag cttccggcct cggtcgaact gatcgtcgcc aacgattctt cgctgccgat
     2101 caaggaagcg gtgcatgacg tccagctcac cctcgccttc tcggtggtgc tggtcatcct
     2161 cgtcatcttc atgttcctga agcgcctgtc cgcgacggtg atcccgaccc tggcgctgcc
     2221 ggtgtcgctg atcggcacct tcgccttcat gtacctgttc gggtattcgc tcgacaacat
     2281 ctccctcttg gcgctgacgc tggcggtcgg cttcgtcgtc gacgacgcca tcgtcatgct
     2341 ggagaacatc gtccgacacg tagaaaaggg catgaagcca ttcgaggctg ccctgaaagg
     2401 cgccagggag atcggcttca ccatcctttc catcaccttc tcgctggtcg ccgtgttcat
     2461 accggtgttc ttcatgagcg gcgtggtggg gcgcgtgttc cgcgagttcg cggttgtaat
     2521 ctcggtctcg atcctcgttt ccggcttcgt gtcgctgaca ctgacgccca tgctctgcgc
     2581 ccgcttcctg aaggaagagg accattcgaa gaaacccggc ctgatctcgc gcggcctcga
     2641 tgccgggttc aatgggctgc tgtggctcta ccgggtgacg ctcgacatcg ccctaaagta
     2701 tcgcctggcc actttggcgc tcaccttcct ttccgcctgg tatgcgttgc acctctaccg
     2761 aaccattccg cagggcttct tcccgctgga agacaccggc ctgatccggg ccagcaccga
     2821 ggggccggca gacacatcct tcgaggcgat ggcggaacgg caactcaagt tgatcgaaat
     2881 cgtcaagaat gacccggcgg tctcgttctt caatgccggt gtcggcggtt tcagcagcgt
     2941 caaccagggc ttcatgttca tttcgctgaa gccaagagcg gagcgtgaca gcgtccagca
     3001 ggtcatcgga cgcctgcggc aggcaacgtc tcagatcccc ggcatcacct tcatcgcggc
     3061 tccggtccag aacctcaatc tgaacggcgg gcgctcctcg cgctcaatgt accagtatac
     3121 gctgacaggc ccgaacttcc aggaactggt ggatctgtca ccaggccatg tgacgaagct
     3181 gcgcgccttg ccccaactgc gcgacgtctc gctcgacctg caactgcgca acccgcagat
     3241 ccgtatggac atcgaccgcg agcaggccca gcgcatgggc gtctcgatgg atcagatccg
     3301 gcagacgctc tacaattcct tcggatcgcg ccagatctcg accatctatt cgcaggcgaa
     3361 cgacttcccg gtgatcatgg aggcggatcg cgccttccag tccagcccgg atgcgctgtc
     3421 gcgcctctac gtgcgcagcc agaatgcgca agtcgcgccg gcacagggca cagcgacgca
     3481 aggcggcaca agcgcggcga cgcagaattt tgctgtcgcc aacattccgc tcgacgccct
     3541 ggtgaagaca tcgtcgaacg tcggcccgct cgccgtgacg cgttcgcagc agctgccggc
     3601 ggtgacgatc tcgttcaaca ccgcgccggg cgtatccctg ggcgaggcgg tggacgccat
     3661 caagcgcgtg gaacgcgaag cgaacctgcc ggcgacggtt tcaacacgct ttgccgggtc
     3721 ggcgcagctg ttcgaggagg ccaaggcagg tcaggcgatg ctgatcctgg cagccgtgct
     3781 ggtgatctat atcctgctcg gtattctcta cgagagcttc atccacccga tcaccatcct
     3841 ctcgggtctg ccgtcggccg gtctcggcgc gctgatcttc ctcaactact acagcatgga
     3901 cctcagtgtg atcgccctga tcggcatcct catgctgatc ggcatcgtca agaagaacgc
     3961 catcatgatg gtggacttcg ccatcgagcg ccgacgggat gggaccgacg ccctgaccgc
     4021 catccgcgag gcttgcctga tccgtttccg tccgatcatc atgacgacgc tggcggcggc
     4081 ccttggcgcc ctgcccatcg ccatgggtat cggcgccggc gcggaactgc ggcaaccgct
     4141 gggcatcgcg gtggtcggcg gtctggccgt ttcacagatc atgacgctct acatcacgcc
     4201 ggtcgtctat ttctatctgg acaagatcga cagcttcttt gccggcaggg aaaccgaggg
     4261 cgccggagct gaattcgccc cggccctaac cgtgattgaa acgccggttg tcgaccagga
     4321 tgctgcacag cccggcgcgg gcgtcgcgcc gaagacctct gccgcggcgc cggcgacgcg
     4381 ttaatttcgg cgcagtaacc tttccagcca accgggacct caggacaatt gaggtcccgg
     4441 tgtcattgtg atacatccct cctcgcgcag cgtttacttt tgattaactc tgcgccgtgt
     4501 ggttggggga gtatgcgtga tgagtggctc ggatatcgtg cgtgcctacc gagaccgttc
     4561 cactggcgca gggcgccgcg caccgggatg gcgcaaaccg gtgatcgccg gcgccgctgc
     4621 agcgagcatc ctggctttcg gcgcgattat cgttcaggcg aacgattccg ccggtgtcta
     4681 tgaagtcaac aagacttaca attccgcccg cgcggcgcgg ccggccgtgt tgccacagat
     4741 cttcagaagc gagccgcggc cggtcgtgca gaccgcgctg agttacgctc cgatattcgg
     4801 tgccctcgcg ccgcatcagg cgcgcggtga ccagaagcgc gccgtgccgc caggatcgcc
     4861 ccgtggcgtt tcggtgtccc ccaaggcgaa gtccaaggat atcgcgcgcg gcacgattga
     4921 tgacggcacg tcctactgcg tgcgcacctg cgacggatac tttttcccgc tgggcaatcc
     4981 ggacgcgggt gacctcgcag cccatgacga gtcctgcgca cgcgcctgcc ccgcttccga
     5041 caccgccgtc tacgtcgcgc cggccggcag ccggggcatc gacgatgcgg tcaaccgccg
     5101 tggcgagcgc tacgagaccc tgcgcaccgc tttcagccat cgcatgcagt ctgacagcgc
     5161 ctgccgctgt acgggaccgg gcaagcagcg caactattcc ctgatggacg atttcacgct
     5221 gcgcagcggc gattatgtga tgtccaccga cggcatgcgc atctatcgcg gtgaggccgg
     5281 ccgggtgcgc ggcccgcgcg acttcgcgcg cgtggacgcc tccaagcttc cggcc
//
LOCUS       DAOMXN010000179         5311 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_8220_length_5311_cov_9.632420, whole genome shotgun sequence.
ACCESSION   DAOMXN010000179 DAOMXN010000000
VERSION     DAOMXN010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 5311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5311
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8220_length_5311_cov_9.632420"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..1285)
                     /locus_tag="PK812_11215"
     CDS             complement(149..1285)
                     /locus_tag="PK812_11215"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRJ70167.1"
                     /translation="MAKDLSSWKGAAAPPGVPLAGRYARLERLDASRHGGDLWQAIAG
                     HDDLWAYMAYGPWRAEAEFVAWLKERQVLADPLYYTVIDGTSGKAIGAITLMEIRPAA
                     GVIEVGNIFFAPALQRTRAATEVIHLAARHVFDDLGYRRFEWKCNNGNERSKRAALRF
                     GFVPEGLFRQHMVVKGRNRDTAWFSIIDSEWPLVRRAFEHWLAPANFDRHGAQKLSLS
                     AINAREMDVGSMVLSRASVLQQAEIEAFQEEAYARNRPILGVEPLPLKWDYATIFSET
                     EVWEARQKGRLVGVLILRPMDDHLVLESVATLPEVQGAGYGNALLNATEFRARDWGFS
                     TIRLLTGEALTQNVDWYRRKGFHVEDVEAIADRNIVHMVKRLVP"
     gene            complement(1293..3113)
                     /locus_tag="PK812_11220"
     CDS             complement(1293..3113)
                     /locus_tag="PK812_11220"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007774996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HRJ70168.1"
                     /translation="MAERKPGSRPTAAKIRKLRSRAWFDNPQNIDMTALYIERMLNFG
                     MTVEELQSGKPIIGIAQSGSDITPCNRHHIQLAQRTKEGIREAGGIAFEFPVHPIQET
                     GKRPTASLDRNLQYLGLVETLYGYPLDAVVLTTGCDKTTPAQIMAAATVDIPAIVLSG
                     GPMLNGWHNGQRTGSGTVLWKAREMLAKGEIGHREFIELVGSSAPSPGHCNTMGTAST
                     MNSIAEALGMSLPGCAAIPAPHRDRQQMAYWTGKRIVEMVWEDLRPSKILTRAAFENA
                     IVVNSAIGGSTNAPIHVNAIARHIGVELTNDDWEAIGYDVPLLVNLQPAGEYLGEEYY
                     RAGGVPAVVAELIEMGRIRREAITVNGRSLHENCAGRRSADREVIKAHDKPMKKAAGF
                     LNMKGNLFDSAIMKTSVISQDFRARYLSNKADPDAFEGVVAVFDGPEDYHKRIDDPKL
                     KLDEHSVLIMRGTGPIGYPGAAEVVNMRPPAYLLKKGIQALPCIGDGRQSGTSGSPSI
                     LNASPEAATGGGLALARSGDRIRVDLKKRRVDLLIPDAEMAARRAELKEKPYRFPASQ
                     TPWQDIQRRFVDELSHGMVLKPAVKYQKIDKRKGIPRDNH"
     gene            3259..4446
                     /locus_tag="PK812_11225"
     CDS             3259..4446
                     /locus_tag="PK812_11225"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRJ70169.1"
                     /translation="MLDIVPAFGRIGEENAFAVLARATELMRQGKDIINLGIGQPDFP
                     TPQNIVEAAIRALRDGHHGYTPATGILPLREAVAADLHRRFSVAVDPDRVMIVPGGKV
                     TMFMAILMFGQPGVEILYPDPGFPIYRSMIEFTGATPVPVPIREANGFAFSAEETLSL
                     ITPRTRLLIVNSPANPTGGVTPKAEVDRLVAGLARYPRVAIMSDEIYDQMTYDGESHV
                     CLLSYPEIADRLILLNGWSKTYAMTGWRMGYSVWPAAYHEAARKLAVNSYSCVNAAAQ
                     WAGLEALTGPQDAVGAMVAEFDRRRKAVVAGLNRLPGVSCIVPKGAFYAFPNIAATGW
                     KAKPLASALLEQAGVATIGGPDFGVHGEGYIRLSYANSLDNIEKALARIGGFLADNRK
                     SKD"
     gene            complement(4609..>5311)
                     /locus_tag="PK812_11230"
     CDS             complement(4609..>5311)
                     /locus_tag="PK812_11230"
                     /inference="COORDINATES: protein motif:HMM:NF016291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF533 domain-containing protein"
                     /protein_id="HRJ70170.1"
                     /translation="QVDQATGAREKLDGFLRQATGGKGTADVFSQLQQMVGQNQGAAG
                     AILGGIGGLLLGTEKGRSIAVDAAKLGGLVLIGGLAYKAYKNYMEGKPLVAFGEPVAP
                     APANSLFNAEAGDGQQNAILMLRAMIASAAADGLIDETERAAILGNVQALGLDQETLD
                     FLEAETANPATAETLASEVQGPEQAAQLYTAARLAVDLDNEYEQQFMADLAAHLGFDA
                     QLVAQLEAAAASVKV"
BASE COUNT          834 a         1828 c         1715 g          934 t
ORIGIN      
        1 ggacgcggcc gccaatgcgg ccaacgcgcg ccgcatctcc ggcgcgcgca gcaaggtgac
       61 ggtgctcgtg ctgccgaccg acgaggagcg ggtcatcgcc gaccagacgc tgggcctcat
      121 cgccggggcc taggccagcc cgcgccgctc acggcaccag ccgcttcacc atgtgcacga
      181 tgttgcggtc ggcgatcgcc tcgacgtctt ccacatggaa gcccttgcgc cggtaccagt
      241 cgacgttctg ggtcagcgct tcgccggtca gcaggcggat ggttgaaaag ccccagtcgc
      301 gcgccctgaa ctcggtggcg ttcagcaagg cgttgccata gcccgcgccc tgcacctcgg
      361 gaagcgtggc gacgctctcc agcacgagat ggtcgtccat cggccgcagg atcagcacgc
      421 cgacgagcct tcccttctgg cgtgcttccc agacctcggt ctcggagaag atcgtggcat
      481 agtcccactt cagaggcagc ggctcgaccc cgaggatcgg ccggttgcgg gcataggcct
      541 cctcctggaa ggcttcgatc tcggcctgct gcagcacgct ggcgcggctg aggaccatgg
      601 acccgacatc catctcgcgc gcgttgatgg cggacagcga cagtttctgg gcgccgtggc
      661 gatcgaaatt cgccggcgcg agccaatgtt cgaacgcccg ccgcaccagc ggccattcgc
      721 tgtcgatgat ggagaaccac gccgtgtcgc ggttccggcc cttgaccacc atgtgctggc
      781 ggaacaggcc ttccggcacg aacccgaagc gcagcgccgc ccgtttcgac cgctcgttgc
      841 cgttgttgca cttccactcg aagcgccggt agcccagatc gtcgaagaca tggcgcgccg
      901 caaggtggat cacctcggtg gccgcccggg tgcgctgaag ggccggcgcg aagaagatgt
      961 tcccgacctc gatcacgccc gccgccgggc ggatctccat gagcgtgatc gcgccaatcg
     1021 ccttgccgga ggtgccgtcg atcaccgtgt aatacagggg atcggcgagg acctgacgct
     1081 ccttcagcca ggccacgaac tccgcttcgg cccgccacgg cccataggcc atgtacgccc
     1141 acaggtcgtc atgcccggcg atggcctgcc acagatcgcc gccatgcctg gatgcatcga
     1201 gacgctccag ccgcgcgtag cgaccggcga gcggcactcc cggcggcgcg gcggcaccct
     1261 tccagctgct caggtccttg gccatcaaac cctcaatggt tgtcgcgcgg aatgcccttg
     1321 cgcttgtcga tcttctggta cttcaccgcc ggcttcagca ccatgccgtg ggacaattcg
     1381 tcgacgaagc gtcgctggat gtcctgccac ggcgtctgcg aggccgggaa ccggtacggc
     1441 ttctccttca gctcggcgcg ccgcgccgcc atctcggcgt ccggaatcag caggtcgacg
     1501 cggcgcttct tcagatccac gcgaatcctg tcgccgctgc gcgccagcgc caggccaccg
     1561 ccggtggcgg cctcgggcga ggcgttgagg atcgagggcg atcccgatgt gccggactgc
     1621 cgcccgtcgc cgatgcaggg cagcgcctgg atgcccttct tcaggaggta ggcaggcggg
     1681 cgcatgttca cgacctcggc cgcgccggga tagccgatcg gcccggtgcc gcgcatgatc
     1741 aggacgctgt gctcatcgag cttcagcttc ggatcgtcga tgcgcttgtg gtaatcctcc
     1801 ggcccgtcga acaccgcgac aacgccctcg aaagcgtcgg gatcggcctt gttggacagg
     1861 tagcgcgcgc ggaaatcctg cgagatcacg ctcgtcttca tgatggcgga atcgaacagg
     1921 tttcccttca tgttgaggaa tccggccgcc ttcttcatcg gcttgtcgtg cgccttgatg
     1981 acctcccggt cggccgaccg cctgccggcg cagttctcgt gcaacgagcg cccgttcacc
     2041 gtgatggcct ccctgcggat cctgcccatc tcgatgagtt cggccaccac cgccggcacg
     2101 ccgccggcgc gataatattc ctcgcccaga tattccccgg ccggttgcag gttcaccagc
     2161 agcggcacgt catagccgat ggcctcccag tcatcgttcg tcagctcgac gccgatgtgc
     2221 cgcgcgatgg cgttgacgtg gatcggcgca ttggtcgaac cgccgatggc ggaattgacg
     2281 acgatggcgt tctcgaaggc ggcgcgcgtc aggatcttgg acgggcgcag gtcctcccac
     2341 accatctcga cgatccgctt gccggtccag taggccatct gctggcggtc gcggtgcggc
     2401 gccggaatcg cggcgcagcc cggcagcgac atgccgagcg cttcggcgat cgagttcatg
     2461 gtcgaggccg tgcccatggt gttgcaatgg ccgggcgagg gcgcggacga acccaccagc
     2521 tcgatgaact cgcgatggcc gatctcgccc ttggccagca tctcgcgggc cttccacagc
     2581 acggtaccgg aaccggtgcg ctggccattg tgccagccgt tcagcatggg accgccggaa
     2641 aggacgatgg ccggaatgtc gacggtggcc gccgccatga tctgggccgg cgtcgtcttg
     2701 tcgcagcccg tggtcagcac cacggcatcg agcggatatc cgtagagcgt ctcgaccagg
     2761 ccgagatact gcaggttgcg gtcgaggctg gcggtggggc gcttgccggt ttcctggatc
     2821 ggatgcaccg ggaactcgaa ggcgatgccg ccggcctccc ggatgccctc tttcgtgcgc
     2881 tgcgccagct ggatgtgatg ccggttgcag ggcgtgatgt ccgagccgga ctgggcgatg
     2941 ccgatgatcg gcttgcccga ctggagctcc tcgaccgtca tgccgaaatt cagcatccgc
     3001 tcgatgtaaa gcgccgtcat gtcgatgttc tggggattgt cgaaccaggc gcgcgagcgc
     3061 agcttcctga tcttcgccgc cgtcggccgc gaacccggtt tcctctcagc catctcatcc
     3121 ccccgcgcgc caggcgcttc atcctgattt gtctggatga taggttcacc ccaccgcgcg
     3181 cgggattgca actggtcaga cggattttgt ctgatatcag acaacctgcc tccctttgct
     3241 ccctgccgga gatctttcat gctcgacatc gttcccgcct ttggccgaat cggcgaggaa
     3301 aacgcctttg ccgtgctggc ccgcgccacc gaactcatgc gccagggcaa ggacatcatc
     3361 aatctgggca tcggccagcc cgacttcccg accccgcaga acatcgtcga ggcggcgatc
     3421 agggccttgc gcgacggaca ccacggctac acgccggcga ccggcatcct gccgctgcgc
     3481 gaggcggtcg ccgctgacct gcaccggcgc ttctccgtcg ccgtcgatcc cgaccgggtg
     3541 atgatcgtgc ccggcggcaa ggtcacgatg ttcatggcca tcctgatgtt cgggcagccc
     3601 ggcgtggaga tcctctatcc agacccgggt tttcccatct atcgctcgat gatcgaattc
     3661 accggcgcga cgcccgtgcc ggtgccgatc cgcgaggcga acggcttcgc cttctccgcc
     3721 gaggagacat tgtcgctgat cacgccgcgc acccggttgc tgatcgtcaa ttcgccggcc
     3781 aatccgaccg gcggcgtcac gccgaaggcg gaggtggaca ggctggtcgc cgggcttgcc
     3841 cgctacccgc gcgtggcgat catgtcggac gagatctacg accagatgac ctacgatggc
     3901 gaaagccatg tctgcctgct gtcttatccc gagatcgccg accggctgat cctgctcaac
     3961 ggctggtcca agacctacgc catgaccggc tggcgcatgg gctattccgt ctggccggcg
     4021 gcctaccacg aggcggcgcg caagctcgcg gtcaattcct attcctgcgt caacgccgcc
     4081 gcgcaatggg ccgggctgga ggcgttgacc ggcccgcagg acgccgtcgg cgccatggtc
     4141 gccgagttcg accggcgccg caaggcggtg gtcgccgggc tcaaccggct cccgggcgtg
     4201 tcctgcatcg tgccgaaagg tgcgttctac gcctttccca acatcgccgc gaccggctgg
     4261 aaggccaagc cgctggcgtc cgcgctgctg gaacaggccg gggtcgccac catcggcggc
     4321 cccgacttcg gggtgcatgg ggagggctac atccgcctgt cctatgccaa ttccctcgac
     4381 aacatcgaga aggcgctggc acggatcggc ggcttcctgg cggacaaccg caagtccaag
     4441 gactgaccgc aagcgacgcg cgggcggggc gaccggcgcc cgcccgcgac gacgccacac
     4501 catcggtcac gacgagtgac cgatgaaggc ctttgaattt tcattacttt tcaaagggaa
     4561 atgaaaattc aaacaggaac cgcgggtcaa tttccgtgac ccttggtctc acaccttgac
     4621 gctggcggcg gccgcctcga gctgggcgac gagctgcgcg tcgaagccga ggtgcgcggc
     4681 aagatcggcc atgaactgct gctcatactc gttgtcgaga tcgaccgcga gtcgcgccgc
     4741 cgtgtagagc tgcgccgcct gctccggacc ctggacctcg gacgcgaggg tttcggcggt
     4801 ggccggattg gcggtttccg cctcaagaaa gtcgagcgtt tcctggtcaa ggccgagcgc
     4861 ctgaacgttg cccaggatcg cggcgcgctc cgtctcgtcg atcagaccgt cggctgcggc
     4921 cgaggcgatc atcgcccgca gcatgaggat ggcgttctgc tggccatcgc cggcctcggc
     4981 attgaacagc gagttggcag gggccggcgc caccggctcg ccgaaagcga ccagcggctt
     5041 gccttccatg taattcttgt aggccttgta ggcgaggccg ccgatcagca cgagcccgcc
     5101 cagcttggcc gcatccaccg cgatcgagcg gcccttctcg gtgccgagca gcaggccgcc
     5161 gattccgccg agaatggcgc cggccgcgcc ctgattctgt ccgaccatct gttgcagttg
     5221 cgagaataca tcggccgtgc ccttgccgcc ggtggcctgg cgcaggaacc cgtcgagctt
     5281 ctcccgcgcg ccggtggcct ggtcgacctg g
//
LOCUS       DAOMXN010000180         5220 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_8437_length_5220_cov_12.184318, whole genome shotgun sequence.
ACCESSION   DAOMXN010000180 DAOMXN010000000
VERSION     DAOMXN010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 5220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5220
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8437_length_5220_cov_12.184318"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="PK812_11235"
     CDS             complement(<1..556)
                     /locus_tag="PK812_11235"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRJ70171.1"
                     /translation="MRFPSVLCIGAAAIDRIATTEARPARLSTSNPGRIRAYPGGAAR
                     NMAEALARLGVPASLAAMIGADSDGDAILASLRGTGVDVTSVQRSSVHATPGYLAIHD
                     GGGDIVLGVADMVAAETAPAALVLDALARLPDALVLADANLGTPALETLARSGRRLAA
                     CAISVEKSQRLRPLLGHIETLFLNR"
     gene            701..1627
                     /locus_tag="PK812_11240"
     CDS             701..1627
                     /locus_tag="PK812_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012329910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRJ70172.1"
                     /translation="MSGMLENIRVFMRAVESGSFSEAGRVLRISSAVASYRIRVLEEH
                     LGCRLLTRTTRRMNLTEAGRIFYERCLDVVEAMERAEASVADEGGAPKGAIKLTAPLG
                     LGRKVIAPIVGQFRDRHPQSEVRLRLSDHLLDLVQEAIDVALRMAVLDDSTFTQRKIA
                     EVRRVICAAPAYLDRRGHPRAPDDLLQHDCLLLRFPGSRQFRWTLTAGSRSLTIPVSG
                     PVDADDGDVLTDWAVAGQGLVLKPRFEVAAHLEAGRLVPVLPDCPPEPVTLAVLYPTR
                     RMMPRQVRDLVDIIHDSSRAHVARELARIGEQ"
     gene            complement(1658..3034)
                     /locus_tag="PK812_11245"
     CDS             complement(1658..3034)
                     /locus_tag="PK812_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HRJ70173.1"
                     /translation="MANAVHPVDEILPAPKLTALGLQHVLVMYAGAVAVPLIVGRALG
                     LTPEQIAFLINADLFACGVVTLIQSFGFPGVGIRLPVMMGVTFASVGPMLAMIASGKA
                     ANAPNEQILNTIYGSVIAAGVFGVVISPFVSRMIRFFPPVVTGTIILIIGISLMRIGV
                     NWAAGAPVDTIPSYGDPIHLGVATFVLLTILALTKYGKGFVSNVAVLLGIIAGSVLAF
                     AIGKMSFAKVATAPWFGLVLPFQFGMPVFDIVSIITMCLVMIVVMIESTGMFLALGSM
                     TGRKLSQDDLTRGLRADGVGTIVGGVFNTFPYTSFSQNVGLVGVTGVRSRWVCVAGGV
                     ILLLLGLTPKLAQLVESVPTFVLGGAGIVMFGMVAATGIRILAAVDYSTNRNNLYIVA
                     ISVGFGMIPLVAPKFFQHMPKALGPLLHSGILLAAIIAVVLNLYFNGVKSQADAEADA
                     AASSHGTE"
     gene            3218..3571
                     /gene="uraH"
                     /locus_tag="PK812_11250"
     CDS             3218..3571
                     /gene="uraH"
                     /locus_tag="PK812_11250"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002966708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HRJ70174.1"
                     /translation="MGRLTTHVLDTANGVPARGVRIDLARIEGDAPIAVGSMVTNADG
                     RTDAPMLAGDTFRPGTYMLTFHVREYFERRAGQTGACPFLDVVPIRFVIADATAHYHV
                     PLLVSPWSFSTYRGS"
     gene            3614..4102
                     /locus_tag="PK812_11255"
     CDS             3614..4102
                     /locus_tag="PK812_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HRJ70175.1"
                     /translation="MKPLACVPLTPEAFAPFGQVIETGGEPITINAGFARRYNDRCTV
                     DVAAGGGETNVSVFVARARILPLSITMMERHPLGSQAFMPLQERPWLAVVCTDPDDRS
                     SYRAFACTGTQGVNYAVNTWHFPLITFVDGDRFLVVDRKGPGNNLEERFLPEDRHLTV
                     VL"
     gene            4118..4948
                     /locus_tag="PK812_11260"
     CDS             4118..4948
                     /locus_tag="PK812_11260"
                     /EC_number="3.5.3.26"
                     /EC_number="3.5.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004037 - allantoicase activity [Evidence
                     IEA];
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional allantoicase/(S)-ureidoglycine
                     aminohydrolase"
                     /protein_id="HRJ70176.1"
                     /translation="MPSYFAPKGGLPPQTQLLTDRAMFTPWYGVIPRGTFSDIVTSYL
                     PFWDKTRLWVIARPMSGFAETFSHYIVEVSAGGGSQRPEADAGAQGVIFVVEGALRLT
                     IAGKDHDLATGGYAYLPAGTRWTLANPHAAPVRFHWIRKRYQAVEGLAAPEAFVTSDQ
                     ATKPIAMPDTNGAWSTTRFVDPTDLRHDFHVNIVNFEPGGLIPFAETHVMEHGLYVLE
                     GKAVYRLNQDWVEVEAGDYMWLRAFCPQACYAGGPGRFRYLLYKDVNRHMPLGPFQGA
                     "
     gene            complement(4955..>5220)
                     /locus_tag="PK812_11265"
     CDS             complement(4955..>5220)
                     /locus_tag="PK812_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRJ70177.1"
                     /translation="ARQMQGGFGGMLSIRLRGGEAAAIGAAARVQLWKRATSLGGVES
                     LIEHRASVEGPGTPCPPDLLRLSTGAEDVDDLFADLDAAIRAT"
BASE COUNT          910 a         1688 c         1732 g          890 t
ORIGIN      
        1 ggcggttgag gaacagggtc tcgatgtggc cgagcaatgg ccggagccgc tgcgactttt
       61 cgaccgatat ggcgcaggcc gccagccgcc gtccggaccg cgccagcgtt tccagtgccg
      121 gcgtgccgag attggcgtcg gccagcacca gggcatcggg caggcgcgcc agggcatcca
      181 agaccagggc cgccggggcg gtttcagcgg caaccatgtc ggcaacgccg agcacgatgt
      241 cgccgccgcc gtcatggatc gccagatagc ccggcgtggc atgaacggag ctgcgctgga
      301 cgcttgtgac atcgacgccg gtgccgcgca gcgaggcaag gatggcatct ccgtcgctgt
      361 cggcgccgat catggcggcg agacttgccg gcacgccgag gcgcgccagc gcttccgcca
      421 tgttgcgggc ggcgcctccc ggataggcgc ggattcgacc cggattcgat gtggaaagcc
      481 gcgcaggccg ggcctcggtc gtggcgatgc ggtcgatggc ggcggcgccg atgcagagca
      541 cggaaggaaa gcgcatggat acagcttgcc gcgcaccgcg ccgattgcaa ccgcattcac
      601 ccggccaggg ccttctccat caggagcgga ctgtgctgtg gattgcagct tccgcttttc
      661 tgcgcttatc ttcaaacagg ttttcgaaaa ggagggcgcg atgagcggca tgctggagaa
      721 tatccgcgtc ttcatgcgag cggtcgaaag cggcagcttt tccgaagccg gccgtgtgct
      781 gcgcatctct tccgccgtcg ccagctatcg catccgggtg ctggaagaac atctgggctg
      841 ccggctgctg acgcgcacca cacggcgcat gaacctcacg gaggccggac gcatcttcta
      901 cgagcgatgc cttgatgtcg tcgaagcgat ggagcgcgcc gaggcgagcg tggcggacga
      961 gggcggcgcg ccgaaggggg cgatcaagct gacggccccg cttgggctcg ggcgcaaggt
     1021 cattgcgccg atcgtcggac agttccgcga caggcatccg cagagcgaag tgcgcctgcg
     1081 tctgtcggac cacctgctcg atctggttca ggaggcgatc gacgtcgcgc tgcgcatggc
     1141 ggtgcttgat gattcgacct tcacgcagcg caagatcgcc gaggtgcgcc gcgtgatatg
     1201 cgccgctccg gcctatctcg accgccgtgg ccatccgcgt gcgcccgatg acctgctgca
     1261 gcacgattgc ctcctgctgc ggtttcccgg atcacggcag ttccgctgga cgctcaccgc
     1321 cggtagccgg tcactgacga taccggtctc tggcccggtc gacgcggacg acggcgacgt
     1381 gctgacggac tgggcggttg ccggccaagg cctcgtgctg aagccgcgct tcgaggtggc
     1441 ggcgcatctg gaggccggtc ggctcgtgcc ggtcctgccg gattgcccgc ccgaaccggt
     1501 cacgcttgcg gtgctctatc cgacccggcg gatgatgccc cgccaggtcc gcgatctcgt
     1561 cgacatcatt cacgacagtt cccgcgccca tgtggcacgg gaactggcgc gtatcggaga
     1621 acagtaggcg gaatttggac gctcccgcct gccggtttca ctccgttccg tgcgagctgg
     1681 cggccgcgtc ggcctcggca tccgcctgag acttcacccc attgaagtag aggttgagca
     1741 cgaccgcgat aatggccgcc agcaggattc cggagtgcaa cagcgggcca agcgccttgg
     1801 gcatgtgctg gaagaacttc ggcgcgacga gcgggatcat gccgaacccg accgagatcg
     1861 cgacgatgta gaggttgttg cggttggtgc tgtagtcgac cgccgcgaga atgcggatgc
     1921 cggtggcggc caccatgccg aacatcacga taccggcgcc gcccagcacg aaggtcggga
     1981 cggactcgac aagctgcgcc agcttcgggg tcagtccgag cagcagcagg atgacgccgc
     2041 cggcgacgca gacccagcgc gaacgcacgc cggtcacgcc gacgaggccg acgttctgcg
     2101 agaacgaggt gtaggggaag gtattgaaca cgccgccgac gatggtgccc acgccatcgg
     2161 ccctcaggcc gcgtgtcagg tcatcctgtg acagtttgcg accggtcatc gacccgagcg
     2221 ccaggaacat gccggtggat tcgatcatca ccacgatcat gacgaggcac atcgtgatga
     2281 tcgagacgat gtcgaacacc ggcatgccga actggaaggg cagcacgagg ccgaaccaag
     2341 gcgcggtcgc caccttggcg aagctcatct tgccaatggc aaaggcgagg accgatccgg
     2401 cgatgatgcc gagcaggacg gcaacgttgg acacgaaacc cttgccgtat ttcgtcaggg
     2461 caaggatggt cagcaggaca aaggtcgcga caccgagatg gatcggatcg ccatagctcg
     2521 ggattgtatc gaccggcgcg cctgccgccc agttgacgcc gatgcgcatc agcgagatgc
     2581 cgatgatcag gatgatcgtg ccggtgacga ctggcgggaa gaaccggatc atccgcgaca
     2641 cgaagggcga gatcaccacg ccgaaaacgc ctgcggcaat gaccgagccg tagatggtgt
     2701 tgaggatttg ctcgttcggt gcgttcgccg ccttgccgga cgcgatcatc gccagcatcg
     2761 gaccgacgga cgcgaaggtc acgcccatca tcaccggcag gcggatgccg acgccgggga
     2821 atccgaagga ctggatcagg gtcacgacgc cgcaggcgaa caggtccgcg ttgatcagga
     2881 aggcgatctg ctcgggagtc agccccaggg cccggccgac gatgagcggc accgccaccg
     2941 cgccggcata catcaccaga acatgctgaa ggcccagcgc cgtcaatttc ggcgcaggca
     3001 gaatttcgtc gacaggatgc accgcattgg ccattgtcgc ctcccccgga aatgccggca
     3061 acgctgcgcg ctccggtctt ggttatgcct ttcttaagtg tctgccgcgc gcgagccctt
     3121 gataagttat gaaattcgcg atcattccaa aaagtatttt gaaatatggg cggcggatcg
     3181 atgctagcgt gcccccgcac gacagggatg ggaaccgatg ggacgactga cgacacatgt
     3241 tcttgatacg gccaacggcg tgccggcgcg cggcgttcgt attgatctgg cgcgcatcga
     3301 aggcgacgcg ccgatcgcgg tcggcagcat ggtgacgaat gcggatggcc ggaccgacgc
     3361 tccgatgctg gccggtgaca cgttccgtcc cggaacctac atgctgacct ttcatgtgcg
     3421 tgagtatttt gaacggcgtg ccggccagac aggcgcctgc ccgttcctcg atgtcgtgcc
     3481 gatccgtttc gtcatcgccg atgccacggc gcattatcac gtgccgcttc tggtatcgcc
     3541 ctggagcttc tcgacctatc gggggtcgtg acccccggct cggagcctcg cgtcgacctt
     3601 caaggagcag cggatgaaac ccctcgcgtg cgttcctctg accccggagg cttttgcacc
     3661 cttcggccag gtcatcgaga cgggcggcga gccgatcacc atcaacgccg gcttcgcgcg
     3721 ccgttacaac gaccggtgca cggtcgatgt cgccgccggc ggcggtgaga ccaacgtcag
     3781 cgttttcgtc gcccgcgcgc gcatcctgcc cttgagcatc accatgatgg agcgccaccc
     3841 attggggtcg caggccttca tgccgctgca ggagcggcca tggcttgccg tggtctgcac
     3901 ggatccggac gatcgttcca gctaccgcgc ctttgcctgc actggcacgc agggcgtcaa
     3961 ctacgccgtg aacacctggc atttcccgct catcaccttc gtggatggcg accgtttcct
     4021 ggttgtcgac cgcaaggggc cgggcaacaa tctcgaagag cggttcctgc ccgaggatcg
     4081 ccatctgacc gtcgtgcttt gaggaatcga caccagcatg cccagctatt tcgccccgaa
     4141 gggtggcctg ccgccgcaga cccagcttct cacggatcgc gccatgttca cgccctggta
     4201 tggcgtcatt ccgcgcggca cgttcagcga tatcgtcacc tcgtatttgc cgttctggga
     4261 caagacgcgc ctatgggtca tcgccaggcc gatgtcgggg ttcgccgaga ccttctcgca
     4321 ctacatcgtg gaggtgtctg cgggcggcgg ctcgcagcgg ccggaagccg atgccggcgc
     4381 gcaaggcgtc attttcgtgg tcgagggcgc cctgcgcctg accattgcgg gcaaggatca
     4441 cgacctggcg accggcggtt acgcctacct gccggccggc acccgctgga ccctggcgaa
     4501 cccgcatgcc gctccggtac ggttccactg gattcgcaag cgctatcagg ccgtcgaggg
     4561 actggccgcg ccggaagctt tcgtcacctc ggatcaggcg acaaagccga tcgccatgcc
     4621 ggacacgaat ggcgcctggt cgacgacgcg cttcgtcgac ccgaccgacc ttcgacatga
     4681 tttccacgta aacatcgtca atttcgagcc gggcgggctg atcccgttcg ccgagaccca
     4741 tgtaatggag cacgggcttt acgtgctgga aggcaaggct gtctaccggc tcaatcagga
     4801 ctgggtcgag gtcgaggccg gtgactacat gtggctgcgc gccttctgcc cgcaggcctg
     4861 ctatgccggc gggccgggcc gttttcgcta cctgctctac aaggacgtca accggcacat
     4921 gccgcttggc ccgttccagg gcgcctgagg tcggttaggt cgcccggatc gccgcatcga
     4981 ggtcggcgaa cagatcatcg acatcctcgg cgccggttga caggcgcagc aaatccggcg
     5041 gacagggcgt gcccggtccc tcgaccgagg cgcgatgctc gatcaggctt tcgacgccgc
     5101 cgagcgaggt tgcgcgtttc cacaactgca cgcgggcggc ggcgccgatg gcggccgcct
     5161 cgccgccgcg caggcggatg gacagcatcc cgccgaagcc gccctgcatc tggcgcgctg
//
LOCUS       DAOMXN010000181         5192 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_8510_length_5192_cov_8.501655, whole genome shotgun sequence.
ACCESSION   DAOMXN010000181 DAOMXN010000000
VERSION     DAOMXN010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5192
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8510_length_5192_cov_8.501655"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..130)
                     /locus_tag="PK812_11270"
     CDS             complement(<1..130)
                     /locus_tag="PK812_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2497 domain-containing protein"
                     /protein_id="HRJ70178.1"
                     /translation="MSTSSAAAQPANRSAEPSMEEILASIRKIIADDQKALDSRAAA"
     gene            complement(275..1708)
                     /locus_tag="PK812_11275"
     CDS             complement(275..1708)
                     /locus_tag="PK812_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HRJ70179.1"
                     /translation="MRLRLRSTAAALAVFAAIGMAPSLRAETIHGALSRAYHANPDLN
                     AQRATTRATDEAVPQALSGYRPRVTGTVDTGVVDQTTRTRRSTTVTNQFTGQRAFSVN
                     HASVTDDIYGPRGVSVALDQVIYNGGRTGNQVRGAESRVLAARETLRNKEQAVLLNAA
                     TAYMNVLRDTAILNLRRNSIEVLSTQLRQTQDRFKVGELTRTDVALSEASLAKARGDA
                     YLAESNLKTSIATYRQHVGDEPKKLSPAQPVEKVLPKSKEAAVAMSQKEHPAIIAALH
                     GVDQQQLQVKVIEGELYPTLSVRALASRRLDSTGNQQESIVASVVGTLSVPIYEGGAV
                     YSRVREAKETLTTQRMTVDLQREAVRQAVVSSWGALETSRAQIEAAQAQVRAAEIALA
                     GVREEAKVGQRTTLDVLTSQQSLLDARVALVSVQRDRVVASYNVLSAIGRLSLRTLGI
                     QVAAYDPKVHYEQVRDKWIGVRTPDGQ"
     gene            complement(1891..2535)
                     /locus_tag="PK812_11280"
     CDS             complement(1891..2535)
                     /locus_tag="PK812_11280"
                     /inference="COORDINATES: protein motif:HMM:NF013315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HRJ70180.1"
                     /translation="MIDYAAARRTMVENQLRTYDVTDQRVLAAFESVPREAFVSDRQR
                     AIAYLDRELTARDDRSILLTPMVLARMLQVLDVQPGETVLDVGGAGYGAALLRDLGAE
                     VTTVVADESAARAGLAESGVAGVTVIQGSLAAGAPNAPHFDAILVHGAADAEPDALIR
                     QLKDGGRLMIVLGRGRSGRATLFRRIGEHVSRHASFDAAAPVAADFRAKPAFQF"
     gene            complement(2645..2718)
                     /locus_tag="PK812_11285"
     tRNA            complement(2645..2718)
                     /locus_tag="PK812_11285"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2684..2686),aa:Cys,seq:gca)
     gene            2916..2990
                     /locus_tag="PK812_11290"
     tRNA            2916..2990
                     /locus_tag="PK812_11290"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2948..2950,aa:Asn,seq:gtt)
     gene            3106..3297
                     /locus_tag="PK812_11295"
     CDS             3106..3297
                     /locus_tag="PK812_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70181.1"
                     /translation="MTAFIASPTTLMFPVRMFNAMRDCLDPPISPVQASLFPFTAMLT
                     RVTDCAPWLRRPYAGESAA"
     gene            3327..4451
                     /locus_tag="PK812_11300"
     CDS             3327..4451
                     /locus_tag="PK812_11300"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRJ70182.1"
                     /translation="MSHGPDADVAVIGGGVVGMSIAYGLGRMGQRVIVLDEGDVAHRA
                     SRGNFALIWVQGKGLNLPDYAVWTLKSADLWAGFAGELERRTGESLAYSRPGGFMTAL
                     SDQELDTRVSDLSRLHNRSPEAARFEQVDRAGMKAMLPEIGPEVVGGTYCPADGHVNS
                     LRLFSALHQATISGGARYRPNAPVETVEARGGGFRIVTAQSEVHAGKVVLAAGLGNAR
                     LAPMVGLDAPVRPQRGQVMVTEKLAPFLRYPCLTVRQTDEGGVMIGDSKEEVGFDSAS
                     HHAVLAAIAARAIRTFPLLGRARVVRSWGALRVMSPDGMPIYDQSETCPGAFLVTCHS
                     GITLAAAHADVIAPAIAEGRLPAGLAVFSARRFSGVSAVA"
     gene            4465..4737
                     /locus_tag="PK812_11305"
     CDS             4465..4737
                     /locus_tag="PK812_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018236693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRJ70183.1"
                     /translation="MTVSIDGRAVEAQAGDSVAAALLAAGVSTFRRTAKSGAARGAYC
                     GMGVCFECLVTVDGIPNQQACLIPVAAGMAIITGQAVPDPVTRRAP"
     gene            4734..>5192
                     /locus_tag="PK812_11310"
     CDS             4734..>5192
                     /locus_tag="PK812_11310"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRJ70184.1"
                     /translation="MNVPSLPGKARGKVCDLLIVGAGPAGMAAALVAVEAGLSVIVAD
                     EGAGPGGQIYRNCLGQSEAINGVLGGDYQKGRALAAPFSSAPVEYLRRTTVFMLEPRP
                     GGGALAGLSCATTGTAQLCAARCVLIATGALERPFPVPGWTLPGVMTAGAG"
BASE COUNT          798 a         1721 c         1772 g          901 t
ORIGIN      
        1 gcgccgccgc ccgggagtcg agcgctttct ggtcatccgc gatgatcttg cggatggagg
       61 cgagaatctc ctccatcgac ggttcggccg aacggttggc gggctgcgcg gctgcggaag
      121 acgtgctcat cgaaaactcc agaacccgag cgggcggcag ggaccgcacg acacaagcat
      181 cagtattcaa atgcgaatcg atgacgcaag attagcgctg aatttcgcag tggatgaaag
      241 cgggtgacga tgcgtcggag caggacctgt tgcgctattg cccgtcgggc gtgcgcacgc
      301 cgatccattt gtcgcgcacc tgttcgtagt gcaccttggg atcataggcc gcgacctgga
      361 tgcccagcgt gcgcaacgac agccggccaa tggccgacag cacgttgtag gaggcgacaa
      421 cccggtcgcg ctgcacggaa accagcgcca cgcgcgcatc gagcagcgac tgctgcgagg
      481 tgagcacatc aagcgtcgtg cgctggccga ccttggcctc ctcgcgcacg ccggccaagg
      541 cgatctcggc ggcccgaacc tgcgcctgcg ccgcctcgat ctgcgcgcgg gatgtctcca
      601 gcgcgcccca ggaactgacc acggcctgac gcaccgcttc gcgctgcagg tccacggtca
      661 tgcgctgcgt cgtgagcgtc tccttggcct cgcgcacgcg ggaatagacc gcgccgccct
      721 cgtagatcgg aaccgacagg gtgccgacca ccgaggccac gatcgattcc tgctggttgc
      781 cggtggagtc caggcgacgg ctggcaagcg cgcgcaccga cagcgtcgga tagagttcac
      841 cctcgatcac cttcacctgc aactgctgct ggtccacgcc atgcagcgcc gcgatgatgg
      901 cggggtgctc cttctgcgac atcgcaacgg ccgcttcctt cgacttcggc agcacctttt
      961 ccaccggctg ggccggcgac agcttcttcg gctcgtcgcc gacatgctgg cgataggtgg
     1021 cgatcgaagt cttcaggttc gactcggcga gataggcatc gccccgcgcc ttggccagac
     1081 tggcctccga caaagcgacg tcggtgcgcg tcaactcgcc gaccttgaag cggtcctgcg
     1141 tctggcgcag ctgcgtcgaa agaacctcga tgctgttgcg ccgcagattg aggatggcgg
     1201 tgtcgcgcag cacgttcatg taggcggtgg cggcgttcag gagcaccgcc tgctccttgt
     1261 tgcgcagcgt ctcgcgcgcc gcgagaacgc gcgattcggc gccgcgcacc tggttgccgg
     1321 tgcgcccgcc gttgtagatg acctgatcga gcgcgacgct gacgccgcgc gggccgtaga
     1381 tgtcgtcggt taccgaggca tggttgacgc tgaacgcgcg ctggcccgtg aactggttcg
     1441 tcaccgtggt cgagcggcgt gtgcgggtcg tctggtccac cacgccggtg tcaaccgtgc
     1501 ccgtcaccct aggccggtag cccgagagcg cctgtggcac ggcctcgtcc gtcgcgcgtg
     1561 tggtggcgcg ttgagcgttg aggtccgggt tggcgtgata ggcacgggac agcgcgccat
     1621 gaatggtctc agcgcgcagc gacggcgcca tgccaatcgc cgcgaacacg gcgagcgccg
     1681 ctgccgtgct ccgcaaccgg agtctcaagc cccacgccac aacgacacgc tccagaaaat
     1741 taacggatcg tttaccttct tacccgggag gcgcgtggtg taaatggcct gagcgcagat
     1801 gtgagtgcgg cacgcggtca gtggcgcaaa aaagcgacag ggattaccga tccgtaatgt
     1861 tcgttgcggc gtcgcgcaaa agcgccagtt ctagaactgg aaggccggtt tggcccgaaa
     1921 atccgccgct acaggcgcgg cggcgtcaaa agacgcgtgg cggctcacat gctcgccgat
     1981 gcggcggaac agagtcgccc gccccgagcg tccgcgtccg agcacgatca tcaggcggcc
     2041 gccgtccttg agttggcgaa tcagcgcatc gggttcggca tcggccgcgc cgtggacgag
     2101 aatcgcgtcg aagtgcggcg cgttcggcgc accggccgcc agcgatccct ggatcacggt
     2161 gacgccggcc acgccggatt cggccagacc ggcccgcgcg gcggactcgt cggccaccac
     2221 cgttgtcacc tcggcgccga gatcgcgcag cagcgccgcg ccataccccg ccccgccgac
     2281 atccagcact gtttcgccgg gctggacatc aagcacctgc agcatccgcg ccagcaccat
     2341 gggcgtcaga aggattgacc tgtcgtcgcg cgcggtcagc tcgcggtcga gataggcgat
     2401 ggcgcgctgc cggtcggaga cgaaggcctc gcgcggaacc gactcgaagg cggcaagcac
     2461 gcgctggtcg gtcacgtcgt aggtgcgcaa ctggttttcg accatggtgc gacgggcggc
     2521 ggcgtaatca atcatggcag ggaccggact ggcttcttga tgaaccgctt ctgcggcagc
     2581 ggcgcgcgaa aagcaagttg gacccttgtc gcgggtcaca ccggcatcac gtcaagcggg
     2641 aaagtggagg cctcgcccag aatcgaactg ggatacaagg atttgcagtc ctctgcgtaa
     2701 ccattccgcc acgaggccaa tcgcggttgg ccccctatag caacgccgcc gcggattcgg
     2761 caaccgcgaa tggcgtttct cgtaaattat tctccttcgc cgccaggcgc ccatgcctgc
     2821 cggcgtgccg attccgccac ggccggcgct ggctaatttt ctcgccaccc catttgcatt
     2881 ccccggcgag cttgctatag gggccgcctc tgctttcccc gatagctcag cggtagagca
     2941 ttcgactgtt aatcgaatgg tcgtaggttc gaatcctact cggggagcca ttttcccaag
     3001 acgcgctcgg aaacgccgcc gggcgcgttt tcttgactga tcatcctgcc gggcctggtg
     3061 cgtggacttg ccttgtcctt cgtccggagc tttggtgaag tgacgatgac ggctttcatc
     3121 gcctcaccca cgaccctgat gtttccggtg cggatgttca acgccatgcg ggattgcctc
     3181 gatccgccga tctccccggt gcaggcatcg ctgttcccgt tcacggccat gctgacgcgg
     3241 gtgaccgatt gcgccccatg gcttcggcgg ccctatgccg gcgagagcgc cgcgtgacct
     3301 cccgagatcc ttccggcgct ggcgccgtgt cccatggtcc cgacgccgat gtcgccgtca
     3361 tcggcggcgg cgtggtcggc atgtcgatcg cctacggtct tggccggatg ggacagcgcg
     3421 tcatcgtcct cgacgagggt gacgtggcgc atcgcgcctc gcgcggcaat ttcgcgctga
     3481 tatgggtgca gggcaagggt ctgaacctgc ccgactacgc ggtctggacc ctgaagtccg
     3541 ccgatctctg ggccggcttt gccggggaac tggagcggag aaccggcgag tcgctcgcct
     3601 attcgcgtcc gggcggcttc atgacggcgc tgtcggacca ggaactggac acgcgcgttt
     3661 cggatctgtc gaggttgcac aaccggtcac ccgaggcggc gcggttcgag caggtggatc
     3721 gcgccggcat gaaggccatg ctgcccgaaa tcggcccgga ggttgtcggc ggcacctatt
     3781 gcccggccga tggccacgtc aattcgctgc ggctgttctc tgcgctgcat caggcgacga
     3841 tttccggcgg ggcgcggtat cggcccaacg ctccggtgga gacggtggaa gcgcgcggcg
     3901 gcggctttcg gatcgtcacc gcgcagagcg aggtgcacgc aggcaaggtc gtgctcgcgg
     3961 cggggctcgg caacgcgcgc cttgcgccca tggtcggtct cgacgcgccg gtgcgcccgc
     4021 agcgcgggca ggtgatggtg acggaaaagc tcgcaccctt cctgcgctat ccctgcctca
     4081 ccgtgcgcca gaccgacgag ggcggcgtca tgatcggcga ctccaaggag gaagtcggct
     4141 tcgacagcgc cagccatcat gcggtgctgg ccgccatcgc agcgcgcgcc atccggacgt
     4201 ttccgctgct ggggcgggcc cgtgtggtgc gcagctgggg cgcgctccgg gtcatgagtc
     4261 cggatggcat gccgatctac gatcagtcgg aaacctgtcc cggcgccttc ctggtgacct
     4321 gtcacagcgg catcaccctg gcggcggccc atgccgacgt gattgcgccg gcaatcgccg
     4381 aagggcggtt gccggccggc ctcgccgtgt tctcagcccg gaggttcagc ggtgtttctg
     4441 cggttgcata ggggcggaac gcccgtgacg gtctcgatcg atggccgagc ggtcgaggca
     4501 caggcgggcg acagcgtcgc cgcggccctc cttgcggcag gcgtttcgac cttccggcgc
     4561 acggcgaaat ccggcgcggc acggggcgcc tattgcggca tgggcgtatg cttcgagtgt
     4621 ctggtgacgg tcgacggcat ccccaaccag caggcctgcc ttattccggt tgccgcaggc
     4681 atggcgatca tcacgggtca ggccgtgccc gatcccgtca cccggcgcgc gccatgaacg
     4741 tcccttctct ccccggcaag gcccggggca aggtctgtga tctgctcatc gtcggcgcgg
     4801 ggccggccgg catggccgcc gcccttgtgg cggtcgaggc cggtctctcc gtcatcgtgg
     4861 cggacgaggg ggcgggcccg ggcggtcaga tctatcgcaa ctgccttggc cagagcgagg
     4921 cgatcaacgg cgtcctgggc ggggattacc agaagggccg cgcgctggcc gcgccgttct
     4981 cgtcggctcc ggtcgagtat ctgaggcgca ccacggtatt catgctggag ccgcggcccg
     5041 gcggcggtgc tctcgccgga ctctcctgcg cgacgacagg cacggcgcag ctctgcgccg
     5101 cgcgttgcgt gctgatcgcg accggtgctc tcgaacggcc tttccccgtg ccgggctgga
     5161 ccctgccggg cgtgatgacg gccggggcgg gg
//
LOCUS       DAOMXN010000182         5191 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_8514_length_5191_cov_11.705413, whole genome shotgun sequence.
ACCESSION   DAOMXN010000182 DAOMXN010000000
VERSION     DAOMXN010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 5191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5191
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8514_length_5191_cov_11.705413"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="PK812_11315"
     CDS             <1..897
                     /locus_tag="PK812_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70185.1"
                     /translation="ASTSCKETEKTALPADAFGFSTGSDVSDLGAWAMGLEYNGAFTG
                     NGFKPGTFASHLGKVQLSTSFVPCWEVGPYFLATTAVGTNRGVIGDLETTTYGFGVEN
                     KYKLLGRATHGVGLTLVFDPNYQSFRSTNTQVTPNLRWSGDMLTSTYKVLLDKELIAG
                     KLFGAVNVQWDQIWTERPGLVNPVAGTSDYARNSVLTLSAALAWQALDGFFVGAETRY
                     VRTHNGLFFNTTTGDALFVGPTAYWQVTKNISASGTWGVQVAGKAKFAVPATTALNLL
                     SSDLNLVTQPQHIAKFKIAVSF"
     gene            951..1493
                     /locus_tag="PK812_11320"
     CDS             951..1493
                     /locus_tag="PK812_11320"
                     /inference="COORDINATES: protein motif:HMM:NF017816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF922 domain-containing protein"
                     /protein_id="HRJ70186.1"
                     /translation="MRAAVLLVALFAAQATAQAAEIRTYVVRGDSAADLIQQMRQLGP
                     RGFWGWTQADYAWTYRYDQSGGRCRIASATVTERIQVTLPSWSNRDAGKACLREAWER
                     MLTRLSDHEQGHVSRWRGTRERIHVALLAIAPQASCDAVAKAADDTAKAEIRRTQDLQ
                     NAYDRQTNHGMALGVALPDC"
     gene            complement(1516..2496)
                     /locus_tag="PK812_11325"
     CDS             complement(1516..2496)
                     /locus_tag="PK812_11325"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRJ70187.1"
                     /translation="MIDRRRMMALGAGALSAAVLTQPASAQTAYPAKTIKIVVPFAAG
                     GGVDVFARLIAEKLRASHNHQVIVENRPGASGTIGGNAVRSSDPDGYTLLFSAGTHVM
                     AQLVLKSAPYDPIRDFTSIARVGEAPMILIMANQRPQKTVPEVVAAFKADPKSWTFAT
                     AALGAPGHVATIQFNTLAGTDIPIVTYPGTGPALNDVAGGHVQLLIDPLLALLPQVRG
                     GKVKGVAVTTAQRTKLAPDIPTMEEVGLKGMDQSSWYGLWGPKGMPAALVTHLNRTVN
                     DAVKDLDKEGKLEKLGIVSTSETPEAFVAFAEAYRDKSAQLLKLGKFEPQ"
     gene            complement(2566..3513)
                     /locus_tag="PK812_11330"
     CDS             complement(2566..3513)
                     /locus_tag="PK812_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRJ70188.1"
                     /translation="MTPMDMRGLSPAPVTAFTRDGEVDHAANARIAKWLVSVKGVKSL
                     VILGHAGEGTFLTEDERLALIRTYVEAVDGAVPIIAGITGEGTRVAAAEARKCKAAGA
                     TGALVYPNHGWLRFGYQKGAPQSRYKAIWEESGLQCILFQYPDVTKACYDLDTQLAIA
                     TQDGVVATKNGVRNMKRWYVEIPELKKANPKLQILSCHDEWLLPTMFDVDGLLVGYGN
                     IAPELLVQLIEAGKAQNYPEARKVFERLLPVTRAVYHRGSHMEGTVALKLGLVHRGIL
                     DHATIREPLKNLGEKAEKEIFAAFDAAGIGQVERALLAA"
     gene            3661..4689
                     /locus_tag="PK812_11335"
     CDS             3661..4689
                     /locus_tag="PK812_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007761994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRJ70189.1"
                     /translation="MPNSPLPTGRVTILDVARAAGVSKSSVSRFLDERLPPSDSEVAR
                     HVRKVAAALGYVRDVSAASLRRGRTGTIGVIVPRLTDTVMAMLYESVARACARSGQFA
                     IVATTDDEPEADRAAARTLMQRGVDGLILSTARADDRLAAELAERDVPFVLALRTDGK
                     SLSSIGDDRLGGYLATRHLVDLGHRRIAIIAGPDYASSSRERVNGYRQAMDEAGLGID
                     PAWIRPSSFSIESGVEAAARLMDLKPAPTAIFAVNDNTAIGAMSTLTRRGLSVPDDVS
                     LVGYNDIPLVSLLPTPLTSVRVPFDQIALAALDLLGAGAGHEKDRVRVATPSLIPRRS
                     TAPPPRHA"
     gene            complement(4714..>5191)
                     /locus_tag="PK812_11340"
     CDS             complement(4714..>5191)
                     /locus_tag="PK812_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ70190.1"
                     /translation="LIAAQDGPRVAALAFDGWDTHANEGGAKGRLATLLGGLDGAFAA
                     FESVLQPIWKDTAVLVITEFGRTARINGTEGTDHGTATVAFLAGGAIRGGRVIADWPG
                     LKTAQLHEGRDLKATTDLRAVMKGLLADHLGLGANILAASVFPESGAVKPMSGLVV"
BASE COUNT          883 a         1751 c         1637 g          920 t
ORIGIN      
        1 gcctccactt cctgcaagga gacggagaag accgccctgc ccgccgacgc cttcggcttt
       61 tccaccggct ccgatgtgtc ggacctcggc gcctgggcga tgggtctcga atacaacggc
      121 gccttcaccg gcaatggctt caagccagga accttcgcca gccatctcgg caaggtgcag
      181 ctctcgactt ccttcgtgcc ctgctgggaa gttgggccat atttcctcgc gacgaccgcc
      241 gtgggcacga accgcggcgt catcggcgat ctcgagacca cgacctacgg tttcggcgtc
      301 gagaacaagt acaagctgct tggccgcgcg acgcacggcg tcggcctgac cctggtgttc
      361 gatccgaact accagtcctt ccgctcgacc aacacccagg tcacgccgaa cctgcgctgg
      421 tccggcgaca tgctgacctc cacctacaag gtgctgctcg acaaggaact gatcgccggc
      481 aagctgttcg gtgcggtcaa tgtccagtgg gaccagatct ggacggaacg ccccggcctg
      541 gtgaacccgg tcgccggcac cagcgactat gctcgcaatt ccgtgcttac gctgtcggcg
      601 gcgctggcct ggcaggcgct ggacggcttc ttcgtcggcg ctgagacgcg ctacgtacgc
      661 acccacaacg gcctgttctt caacaccacc accggcgacg cgctgttcgt cggcccgaca
      721 gcctactggc aggtgacgaa gaacatctcg gcctcgggca cctggggcgt gcaggtcgcc
      781 ggcaaggcga aattcgccgt tcccgccacc accgccctca acctgctttc gagcgacctg
      841 aacctggtga cgcagcccca gcacatcgcc aagttcaaga tcgccgtcag cttctgaaaa
      901 acctgttcgg ggtcttgaca cccgtcctgc ggcgcggaac caacggcggc atgagagccg
      961 ccgttcttct tgtggcgctg tttgctgcgc aggcgaccgc acaggctgcc gaaattcgca
     1021 cctatgtggt gcgcggtgac agcgccgctg acctcatcca gcaaatgaga cagctcggtc
     1081 ccagaggatt ctggggctgg acgcaggcgg actatgcctg gacgtaccgc tacgaccaat
     1141 ccggcgggcg ctgccgcatt gcttcggcca cggttaccga acgcatccag gtgacgcttc
     1201 cgtcgtggtc gaaccgcgac gcgggcaagg catgcctgcg ggaggcctgg gaacggatgc
     1261 tcacccggct ctcggaccac gaacaggggc acgtcagccg ctggcgcggc accagggagc
     1321 gcatccatgt cgccttgctg gcaatcgcgc cgcaggcgtc ctgcgatgcg gtggccaagg
     1381 cggctgacga caccgccaag gccgaaatcc gccgcacgca ggacctgcag aatgcctatg
     1441 accggcagac gaaccacggc atggcccttg gtgtcgccct gccggactgt tgacgcccgc
     1501 acatcgctcc gcgcgttatt gcggctcgaa cttgccgagc ttgagcaact gcgcgctctt
     1561 gtcccggtac gcttcagcga acgcaacaaa ggcttccggc gtttcggatg tcgagacaat
     1621 gccaagcttt tcgagcttgc cctccttgtc gagatccttc accgcgtcgt tcactgtccg
     1681 gttgaggtgc gtgaccagtg cggccggcat gccttttggg ccccagagcc cgtaccagga
     1741 ggattgatcc atgcccttga ggccgacttc ctccatggtc gggatgtccg gcgctagctt
     1801 ggtgcgctgg gcggtcgtga cggcgacgcc cttcaccttg ccgccgcgga cctgcggcaa
     1861 cagggccagc agcggatcga tcagcaactg cacatgaccg cccgcgacgt cgttgagcgc
     1921 cggcccggta ccgggatagg tgacgatcgg aatatccgtc cccgccagcg tgttgaactg
     1981 gatggttgcc acgtgacccg gcgcgccgag agccgccgtg gcgaaagtcc aggacttcgg
     2041 atcggccttg aacgccgcca cgacctcggg gaccgttttc tgcggccgct ggttcgccat
     2101 gatgaggatc atgggcgcct cgccgactcg ggcgatcgac gtgaaatccc ggatcggatc
     2161 gtagggcgct gacttcagca caagctgggc catcacatgc gtgccggccg aaaacagcag
     2221 cgtgtagccg tccggatcgg acgaacgcac tgcgttgccg ccgattgtac cggaagcccc
     2281 gggcctgttc tcgacgataa cctggtggtt atgcgacgcg cgcaactttt cggcgatcag
     2341 ccgcgcgaac acgtcgacgc caccaccggc agcgaacggc acaacaatct tgatggtctt
     2401 ggcggggtaa gcggtctgcg cagaggctgg ctgcgtcagg actgcggcac tcagtgcacc
     2461 ggcacccagc gccatcatgc gacgacggtc aatcatcggt tcctcccatg aagacagggc
     2521 ctgcggcgcg ggcgtgtgcg gcgtcgcagg cgagtgagac gtcgatcagg ccgcgagaag
     2581 ggcgcgctcg acctgcccga tcccggccgc atcaaaggcg gcgaaaatct ctttctcggc
     2641 tttctcgccg aggttcttca gcggctcgcg gatcgtggca tggtcaagaa tcccgcgatg
     2701 gacaaggccg agcttgagcg ccacggtgcc ctccatgtgc gagccacgat gatagaccgc
     2761 acgcgtgacc ggcaggaggc gctcgaacac cttgcgcgct tccggatagt tctgggcttt
     2821 cccggcctcg atcagttgaa ccagcagttc cggagcgatg ttgccgtaac cgacgagcaa
     2881 gccatcgaca tcgaacattg tcggcagcag ccactcgtca tggcaggaaa ggatctgcaa
     2941 cttcggattg gccttcttca gctccggaat ttcaacgtac cagcgcttca tgttgcgcac
     3001 gccgttcttg gtggcgacca ccccatcctg ggtggcgatc gcaagctgcg tgtcgagatc
     3061 gtagcaggcc ttggtgacgt ccggatactg gaacaggatg cactgcagac cggactcttc
     3121 ccagattgcc ttgtaccggg actgcggcgc gcccttctga tagccaaagc gcagccagcc
     3181 gtggttggga tagacaagcg cgccagttgc gccggcggcc ttgcacttgc gggcttctgc
     3241 ggcggcgacg cgtgtaccct cgcccgtgat gcccgcgatg atcggcaccg cgccgtctac
     3301 ggcttccaca taggtgcgga tcaacgccag ccgctcgtcc tcggtcagga acgtgccttc
     3361 gccggcatgg ccgaggatga caaggctctt cacgcccttc accgaaacca gccacttggc
     3421 aatgcgggca ttcgcggcgt ggtcgacttc gccgtcgcgg gtaaaggctg tcaccggggc
     3481 ggggctgagg ccacgcatgt ccatcggcgt catgacatat ctcctgtggg tcgatgctcc
     3541 cgcgcaatcc gtcggggaac gttattggga acgttccctt tcttgcattg caaacgttcc
     3601 ctgtcaaggc tagagtcact gtgtcgtgat gcttcccaaa atttggatct gtgaccacag
     3661 atgcccaact ccccacttcc aaccggtcgg gtgaccattc tcgacgtggc gcgcgccgca
     3721 ggcgtgtcga aatcgagcgt ctcgcgcttc ctggacgagc ggctgccgcc ctccgacagc
     3781 gaggtggctc gccacgtccg aaaggttgcg gccgccctgg gctatgtccg cgatgtctct
     3841 gcggccagtc tgcggcgcgg aagaaccggc accatcggcg tcatcgtgcc gcgcctgacc
     3901 gatacggtca tggccatgct gtacgaatcg gtggcccgtg cctgcgcccg ctctggacag
     3961 tttgcgatcg tcgccaccac cgacgatgag ccggaggcgg accgcgccgc tgcccgcacc
     4021 ctgatgcagc gcggtgtcga cgggctgatc ctgtcgacag cgcgtgcgga tgacaggctt
     4081 gccgccgaac tggccgaacg cgacgtgcct ttcgtgcttg ccctgcgcac tgacgggaag
     4141 agcctctcat cgatcgggga cgacaggctg ggcggctacc tcgcgacccg gcatctggtc
     4201 gatctgggcc accgcaggat cgcaatcatc gccgggcccg actatgcctc cagcagccgc
     4261 gagcgcgtga acggctacag gcaggcgatg gacgaagccg ggctggggat cgatccggcc
     4321 tggatccggc cctcgagctt cagcatcgaa tccggcgtcg aggctgccgc gcgccttatg
     4381 gacctgaagc ctgcgccaac ggccattttt gcggtcaacg acaatacggc gatcggcgca
     4441 atgtcgacac tgacaaggcg ggggctgtct gtgcctgatg acgtctcgct ggtcggctat
     4501 aacgacatcc cgcttgtcag ccttctgccg acgccgctga cctccgtacg cgttcccttt
     4561 gatcagatag cccttgccgc cctcgatctc ctcggcgcag gagcgggcca cgagaaggat
     4621 cgcgtgcgcg tcgccacgcc gtcccttatc ccgcgacgct ccactgcgcc gcctccgagg
     4681 cacgcctgag ggcaaacgct tgggcgcgag acgctaaacc accagcccgg acatcggttt
     4741 caccgcgccg gattccggga agacgctggc cgccaggatg ttggcgccga ggccgagatg
     4801 atcggcgagc agccccttca tcacggcgcg caggtccgtg gtcgccttga ggtcgcggcc
     4861 ttcgtgcagt tgcgctgtct tcaggcccgg ccagtccgcg atgacgcgac cgcccctgat
     4921 cgcgccgccc gcgaggaacg caacggtcgc ggtgccgtga tcggtgcctt ccgtgccgtt
     4981 gatgcgcgcg gtgcggccga actcggtgat caccagaacc gccgtatcct tccagatcgg
     5041 ctgaagcacg gattcgaacg cggcgaacgc gccatcgaga ccgccgagca gcgtcgcgag
     5101 ccggcctttc gcgcccccct cgttggcgtg cgtatcccaa ccgtcgaagg cgagcgcggc
     5161 gacgcgcggc ccgtcctgcg ccgcgatcag c
//
LOCUS       DAOMXN010000183         5153 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_8596_length_5153_cov_12.212044, whole genome shotgun sequence.
ACCESSION   DAOMXN010000183 DAOMXN010000000
VERSION     DAOMXN010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8596_length_5153_cov_12.212044"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="PK812_11345"
     CDS             complement(<1..213)
                     /locus_tag="PK812_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70191.1"
                     /translation="MRFGSALPIDEVLDLLDRTLSSQVAAVLVAPPGAGKTTRVPLAL
                     LEAPWLGDRRIVMLEPRRLAARGAASR"
     gene            complement(220..1533)
                     /locus_tag="PK812_11350"
     CDS             complement(220..1533)
                     /locus_tag="PK812_11350"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012321525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HRJ70192.1"
                     /translation="MNIAMIGSGYVGLVSGACLADFGHVVTCVDKDSAKIDRIKAGDM
                     PIYEPGLAELVQSNVEESRLSFTTDLAAAVSDAEAVFIAVGTPSRRGDGQADLTFVYA
                     AAREIAAAIKGFTVVVTKSTVPVGTGDEVERIIREARPDAEFAVVSNPEFLREGAAIS
                     DFKRPDRIVVGTLDERARSVMSEIYRPLYLNQAPIVFTSRRTSELIKYAGNAFLAMKI
                     TFINEMADLCEKVGANVQEVARGIGLDNRIGSKFLHAGPGYGGSCFPKDTLALVQTAQ
                     AYGSPVRIVETVVEVNDARKAALAERVVAACGGSVRGKRIAVLGLTFKPNTDDMREAP
                     SLTLVPALIAAGASVSAYDPEGMELAAPMLPGVTFARNAYDCCEGADALVIVTEWDAF
                     RALNLTRVKAALKSPTVIDLRNIYRPEDMVARGFAYTSIGRPQAS"
     gene            complement(1561..3846)
                     /locus_tag="PK812_11355"
     CDS             complement(1561..3846)
                     /locus_tag="PK812_11355"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HRJ70193.1"
                     /translation="MSEMEPLKRRSRPNITDQEALDFHSRGKPGKLEIVPTKPMATQR
                     DLSLAYSPGVAVPVKAIAQDPATAFDYTARGNIVAVISNGTAILGLGNLGALASKPVM
                     EGKSVLFKRFADIDSIDLEVDTEDPDAFINAVRYLGPSFGGINLEDIKAPECFIIEER
                     LRELMDIPVFHDDQHGTAIIAAAGLLNALQITGRDVKSTKLVVNGAGAAGIASVELLK
                     AMGFAPNNVILCDTKGVVYRGRKEGMNQWKSAHAAETKARTLSDAMTGADCFFGLSAK
                     GALTPDMVRSMAPNPIIFAMANPDPEILPEEVADIRDDAIMATGRSDYPNQVNNVLGF
                     PYIFRGALDVRATTINMEMKIAASYALADLAREDVPDEVAAAYGGVRPRFGRDYIIPV
                     PFDPRLMSKIPAAVARAAMDTGVARKPLTSLAKYEAALSARRDPVAGVLSRVFEQVRR
                     NPKRVVFAEGEEEAVIRAAVSFVNQGLGHAILVGREDRVKAMASQAGIEIKDGLEIHN
                     ARLSTRNAVYAQFLYARMQRKGLLFRDCQRLINQDRNYFAASMVAMGDADAMVTGLTR
                     NFSVTLADVRRVVDVKPGHSVIGVSMILSRGRPVIVADTAVHDMPTAEELCAIAVEAA
                     GFARRLGYVPRVALLAFSTFGYPESERSQRVREAVQLLDRRKVDFEYDGEMGADVALN
                     PDMMAAYPFCRLSGPANVLIMPAFHSAAIATKMLQELGGSTVIGPLLVGLDKPVQIVS
                     INSKDSDIVNAAALAASGVGG"
     gene            complement(3979..4605)
                     /locus_tag="PK812_11360"
     CDS             complement(3979..4605)
                     /locus_tag="PK812_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70194.1"
                     /translation="MLAMLIQPVSAQSLPEGMTACSISGYSLDRSRAGTPVRAEPNAK
                     AKIVGRIAPPQKATARDLEDISVPTDEVWRAGFQILGIKDGWVLIEKALHPYNDPYYF
                     GTLGRRSTGGIKTYSGRGWLPLSQIGGKVTYYRKMPLGALYAEPREDAPLLPAKNAME
                     GPLSGGNQPKRILACSGEWVQVESPDGVIGWWRGLCGAPVSDCEPLSG"
     gene            complement(4647..>5153)
                     /gene="ugpC"
                     /locus_tag="PK812_11365"
     CDS             complement(4647..>5153)
                     /gene="ugpC"
                     /locus_tag="PK812_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007799524.1"
                     /note="part of the UgpABCE glycerol-3-phosphate uptake
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HRJ70195.1"
                     /translation="LKVTSIYVTHDQVEAMTLADMIVVMNGGQVEQIGTPSEVYTRPE
                     STFVATFIGSPAMNMLKGSIAGKGMVDVGGVKLACDTGAAAAGTSVLVGIRPDETSPS
                     PGGAIGVDVDFVEELGPSRLYHGTFGGQDFTLQLSPHAADAQAKSVSVDVPARAVHLF
                     DAASGRRI"
BASE COUNT          897 a         1680 c         1673 g          903 t
ORIGIN      
        1 gcgggaggcg gcgccccgcg ccgccagacg ccgtggctcc agcatgacga tgcggcgatc
       61 gccgagccag ggcgcctcaa gcagggccag cggtactcgg gtcgtcttcc ccgctcccgg
      121 aggcgcgaca agaaccgccg caacctggct cgacagcgtc cggtccagaa gatcgaggac
      181 ctcgtcgatg ggcagcgccg aaccgaagcg catgccgtgc tagctggcct gcgggcggcc
      241 gatgctggtg taggcgaaac cgcgcgccac catgtcttcc ggacgataga tgttgcgcag
      301 gtcaatgacg gtgggcgact tcagcgccgc cttgacgcgc gtcagattca gcgcgcggaa
      361 cgcgtcccat tccgtcacga tgaccagcgc gtccgcgcct tcgcagcaat cgtaggcgtt
      421 cctggcgaag gtcacgcccg gcagcatcgg cgctgccagt tccatgccct cgggatcata
      481 ggcgctcacg ctggcgcccg ccgcgatcag cgcgggaacg agcgtcagcg acggcgcctc
      541 gcgcatatcg tcggtgttcg gcttgaaggt cagaccaagc acggcgatgc gcttgccgcg
      601 aacgcttccg ccgcaggccg cgaccacgcg ttcggccaat gcggccttgc gggcatcgtt
      661 gacttcaacc acggtctcca cgatgcgcac cgggctgcca taggcctgcg ccgtctgcac
      721 aagggcaagt gtgtccttgg gaaagcagga gccgccatag cccgggccgg catgcaggaa
      781 cttcgaaccg atgcgattgt cgaggccgat gccgcgggcg acttcctgaa cgttggcgcc
      841 caccttctcg cacaggtccg ccatctcgtt gatgaaggtg atcttcatgg cgaggaaggc
      901 atttccggca tacttgatca gctcggatgt gcggcgggag gtgaagacga tcggcgcctg
      961 gttcagatag agcggacgat agatttcgct catgaccgaa cgggcgcgtt cgtccagcgt
     1021 gccgacgacg atgcggtcag gccgcttgaa gtccgagatc gcggcgcctt cgcgcaggaa
     1081 ttcgggattg gacaccacgg cgaactcggc gtccggccgc gcctcgcgga tgatccgttc
     1141 gacttcatcg cccgttccga ccgggaccgt ggacttcgtg acgaccacgg tgaagccctt
     1201 gatggccgcg gcgatctccc gggcggcagc gtagacgaag gtgagatccg cctggccgtc
     1261 gccgcggcgc gacggcgtgc cgaccgcgat gaacacagct tccgcatccg aaaccgcagc
     1321 cgccaggtcc gtggtgaacg acagccggct ttcctcgaca ttcgactgca cgagctcggc
     1381 cagacccggc tcatagatcg gcatgtcgcc ggccttgatc cgatcgatct tggccgaatc
     1441 cttgtcgacg caggtcacca catggccgaa gtcggccagg caggcgccgg acaccagccc
     1501 aacgtatccc gagccgatca ttgcgatatt catgacaggg ttcctttctg gaagcccgct
     1561 ctagccgcct acacccgacg ccgcaagcgc cgccgcgttg acgatatcgc tgtccttcga
     1621 attgatggag acgatctgca cgggcttgtc gagacccacg agcagcggtc cgatcacggt
     1681 ggaaccaccc agttcctgca gcattttcgt ggcgatggcg gcggaatgga aggccggcat
     1741 gatcagcacg ttcgccgggc cggacaggcg acagaacgga taggcggcca tcatgtcggg
     1801 gttgagagcg acgtccgccc ccatctcgcc gtcgtattcg aaatcaacct tgcgtctgtc
     1861 gagaagctgg accgcctcac gcacgcgctg agaacgctcg ctctcgggat agccgaaggt
     1921 cgagaaggcc aagagcgcca cgcgtggaac atacccgagc cggcgggcga atccggcggc
     1981 ctcgacggcg atggcgcaca attcctccgc cgtcggcatg tcatgcaccg ccgtatccgc
     2041 gacgatcacc ggacgtccgc gcgacaggat catggacacg ccgatcacgc tgtgaccggg
     2101 cttgacgtca acgaccctgc gcacatcggc gagcgtgacc gagaagttgc gcgtcaggcc
     2161 agtcaccatc gcatccgcgt cgcccatggc caccatggag gcggcgaagt agttgcggtc
     2221 ctgattgatc agccgctggc agtcacggaa caaaaggccc ttgcgctgca tgcgcgcgta
     2281 gaggaactgg gcgtaaacgg cattgcgcgt cgacaggcgc gcgttgtgga tttcgagccc
     2341 gtccttgatc tcgatgccgg cctggctggc catcgccttc acccggtcct cacggccgac
     2401 caggatggca tggcccaaac cctgattgac gaaggacacg gcggcgcgga tcaccgcttc
     2461 ttcctcgcct tccgcgaaga cgacgcgctt gggattgcgc ctgacctgct cgaagacgcg
     2521 cgacagcacg ccggcgaccg gatcgcggcg cgccgacagc gccgcttcgt acttggcaag
     2581 actggtgagc ggcttgcgag caacaccggt gtccatcgcg gcgcgcgcca cggcagcagg
     2641 aattttcgac atcaggcgcg gatcgaaggg caccgggatg atgtagtcgc gcccgaagcg
     2701 cgggcgaacg ccgccatagg ccgcggcgac ctcgtccggc acgtcctcgc gcgccagatc
     2761 ggcgagcgcg taggaggcgg cgatcttcat ttccatgttg atggtcgtcg cccgaacgtc
     2821 gagcgcgccg cggaagatgt agggaaagcc cagcacgttg ttgacctggt tcgggtaatc
     2881 cgaacggccc gtcgccatga tggcgtcatc gcgaatgtcc gcgacctctt ccggaaggat
     2941 ttccggatca ggattggcca tggcgaagat gatgggattg ggcgccatgg agcgcaccat
     3001 gtcgggcgtc agggcgccct tggcggaaag gccgaagaag cagtcggcgc cggtcatggc
     3061 gtccgagagc gtgcgcgcct tggtttccgc cgcgtgcgcg ctcttccact ggttcatgcc
     3121 ttccttgcgc ccgcgataga cgacgccttt ggtgtcgcac aggatgacgt tgttgggcgc
     3181 aaagcccatg gccttgagca gttccaccga ggcgatgccc gccgcgcccg cgccgttgac
     3241 cacgagtttc gtcgacttga cgtcgcgccc tgtgatctgc agggcattga gcaggccggc
     3301 tgcggcgatg atggccgtgc cgtgctggtc gtcatggaac accgggatgt ccatgagttc
     3361 gcgcagccgc tcctcgatga tgaagcattc cggggccttg atgtcctcca ggttgatgcc
     3421 gccgaaggag gggccgaggt agcgcaccgc gttgatgaag gcgtccgggt cttcggtgtc
     3481 gacttccaga tcgatggaat cgatgtcggc gaaacgcttg aacagcaccg acttgccttc
     3541 catcaccggc ttggaggcga gcgcgccgag attgccgaga ccgaggatgg ccgtgccgtt
     3601 ggaaatcacg gcgacgatgt tgccgcgcgc tgtgtagtcg aaggccgtgg ccggatcctg
     3661 cgcgattgcc ttcacgggga ctgcgacacc cggcgaatag gccagcgaca gatcgcgctg
     3721 cgtcgccatc ggcttggtcg gcacgatctc gagcttgcca ggcttgccgc gcgagtggaa
     3781 atcaagcgcc tcctgatccg tgatgttcgg gcggctgcgc cgcttcagcg gctccatctc
     3841 ggacatgcgt gtttcccttg tcagtgttct tggcaagatc ggcccagctc gatcaagaag
     3901 cttgccataa aggtgcaggc tggcaacctc aagcgaccgc gtctgcgctt atggtaggtc
     3961 tcccctgcgc cggtcgcgtc agccgctcaa gggttcgcag tcgctgaccg gggcaccaca
     4021 caggccccgc caccagccaa tgacgccgtc ggggctttcc acctgcaccc attcgccaga
     4081 gcaggcgagg atccgtttcg gctggtttcc gccgctcagc gggccctcca tggcgttttt
     4141 tgccggcaag agcggagcgt cctcccttgg ctccgcgtac aaggcgccaa gcggcatctt
     4201 gcgatagtag gtcaccttgc cgccgatctg actcagcggc agccagccgc gccctgaata
     4261 ggtcttgatg ccgccggttg agcgccggcc cagtgtgccg aagtagtagg gatcgttgta
     4321 ggggtgtagc gccttttcta tcagcaccca gccatccttg atgccgagaa tctggaaccc
     4381 ggcccgccag acctcgtctg taggcacgga gatgtcttcc agatcccttg cggtcgcctt
     4441 ttgcggcggc gcaatgcggc cgacgatctt ggccttggca ttgggctcgg cacgcactgg
     4501 cgtgcctgca cggcttctgt cgagcgaata gccggaaatc gagcaggccg tcatgccttc
     4561 cggcagggac tgcgcagata ccggctgaat aagcatagcc agcatcagtg caccaagggt
     4621 tggtactgcg gcaccaatgc ttgccatcag atcctccgcc cgctcgcagc atcaaacaaa
     4681 tgcaccgccc tcgccggcac gtcgacgctg acgcttttgg cctgggcatc cgccgcgtgt
     4741 ggcgacagct gcagggtgaa gtcctggccg ccgaaggtgc cgtgatagag gcgcgacggt
     4801 cccagctcct cgacgaaatc cacgtccacg ccaatggctc cgcccggcga ggggctggtt
     4861 tcgtcaggcc tgatgccgac gagcaccgaa gtcccggcgg cagccgcgcc ggtgtcgcag
     4921 gccagcttca cgcccccgac gtcgaccatg cccttaccgg cgatgctgcc cttcagcatg
     4981 ttcatcgccg gagaaccgat gaacgtggcg acgaacgtgc tttccggccg ggtatagacc
     5041 tcgctcggcg tgccgatctg ctcgacctgc ccgccgttca tcaccacgat catgtcggcg
     5101 agggtcatcg cctcgacctg atcgtgcgtg acgtagatcg aggtgacctt gag
//
LOCUS       DAOMXN010000184         5105 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_8707_length_5105_cov_11.570693, whole genome shotgun sequence.
ACCESSION   DAOMXN010000184 DAOMXN010000000
VERSION     DAOMXN010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 5105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5105
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8707_length_5105_cov_11.570693"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2556)
                     /gene="alaS"
                     /locus_tag="PK812_11370"
     CDS             complement(<1..2556)
                     /gene="alaS"
                     /locus_tag="PK812_11370"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HRJ70196.1"
                     /translation="MSGVNEIRSTFLDFFAKNGHEVVASSPLVPRNDPTLMFTNAGMV
                     QFKNVFTGQEHRPYSRAVTSQKCVRAGGKHNDLDNVGYTARHHTFFEMLGNFSFGDYF
                     KDRAIELAWTLVTREYGLPKDKLMVTVYHEDDEAFGLWKKIAGLPDERIIRIPTSDNF
                     WAMGDTGPCGPCSEIFYDHGPHIPGGPPGSADADGDRFIEVWNLVFMQFEQLPGGERV
                     RLPKPSIDTGMGLERISAVLQGTHDNYETDMMRSIIRTVADLVSVDAEGPQKASHRVI
                     ADHLRASAFLVADGVLPSNEGRGYVLRRIMRRAMRHAQLLGAREPLMHRLVHTLVREM
                     GQAYPELHRAEALIAETLKLEEGRFRRTLERGLSLLEDEAAGLTSGATLKGETAFRLY
                     DTYGFPLDLTQDALRARGIGVDVDGFNAAMARQKAEARAAWAGSGEAATEAVWFAIKE
                     RAGATEFLGYDTEAAEGVVTALIRDGAEVSALKAGETGFVVLNQTPFYGESGGQVGDA
                     GAMTGEGVRLSVNDTQKRLGEVFAHAVRVEEGVLKVGAALNLAVDTRRRSAIRANHSA
                     THLLHEALRHVLGDHVAQKGSLVSPERLRFDISHPKPVNDDEIGQVEDLANALVLQNA
                     PVVTRLMAVDEAIASGARALFGEKYGDEVRVVSMGRYEEGEPAGKAYSVELCGGTHVQ
                     RTGDIGLIAIVAEGAVSAGVRRIEALTGSAARRHLNGESRRLQAVSAALRAPAVEAAE
                     RVIQLMDERRKLERELADARKKLAMGGGAAAAGDDVREVGGVKLLARVLDGIDARDLK
                     SLADEGKKRLGSGVVALAGVSADGKAGLVVAVTDDLTARFSAVDLVRKAVGPLG"
     gene            complement(2719..3804)
                     /gene="recA"
                     /locus_tag="PK812_11375"
     CDS             complement(2719..3804)
                     /gene="recA"
                     /locus_tag="PK812_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HRJ70197.1"
                     /translation="MTQPALRLVEGSSMDKTKALDAALSQIERAFGKGSIMRLGKNQK
                     AIEIETVSTGSLGLDIALGVGGLPRGRVIEIYGPESSGKTTLALHTIAEAQKKGGICG
                     FIDAEHALDPVYARKLGVDLENLLISQPDTGEQALEITDTLVRSGAVDVLVVDSVAAL
                     TPKAEIEGEMGESQPGLQARLMSQALRKLTASISRSNTMVIFINQIRMKIGVMYGSPE
                     TTTGGNALKFYASVRLDIRRIGAIKEREEVVGNTTRVKVVKNKVAPPFKQVEFDIMYG
                     EGVSKMGELVDLGVKAGIVEKSGAWFSFDSQRLGQGRENAKAFLRQNAEIAGRIEQMI
                     RQNAGLIAERILENGGAEPEDDGPPEH"
     gene            4201..4641
                     /locus_tag="PK812_11380"
     CDS             4201..4641
                     /locus_tag="PK812_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70198.1"
                     /translation="MGFAVSSGVASAGERVLSTVGSWRVILNLNPDNSFNRCLIETTQ
                     GGQMLRIAGGAKSTDLSLSVPAMGIPNGGQGTIGSDGMQPEYFTFAANKLRAWTAIPW
                     EMGLHLEKARSIEVGVNRRQMKWRVSGTKQAMIRMTDCVNQNAP"
     gene            complement(4693..>5105)
                     /locus_tag="PK812_11385"
     CDS             complement(4693..>5105)
                     /locus_tag="PK812_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012319197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRJ70199.1"
                     /translation="RPAGETKAPAADLTGKGTIMLVEDEEAVRNFAARALSSRGYTVL
                     QAPSGLDGLELAKDRLKDIDLVVSDVVMPEMDGPTMLGELRKLAPDLKVIFVSGYAEE
                     AFRKNLPEGQQFDFLPKPFSLKQLIEKVKTVMEG"
BASE COUNT          843 a         1796 c         1541 g          925 t
ORIGIN      
        1 gccaagcggc ccgacggcct tgcgcaccag gtccaccgcg ctgaaccgcg ccgtcagatc
       61 atccgtgacg gccaccacca gccccgcctt gccatcggcg gagacgccgg ccagcgccac
      121 cacgcccgat ccgagccgct tctttccctc atcggcgagg ctcttcaggt cgcgcgcgtc
      181 gatgccgtcc agcacgcgcg ccagcagctt caccccgccg acttcgcgca catcgtcgcc
      241 cgctgcggcg gcgccccctc ccatggcgag cttcttgcgc gcgtcggcaa gctcacgttc
      301 cagcttgcgc cgctcatcca tgagctggat cacgcgttcg gccgcctcga cagccggagc
      361 gcgcagcgcc gccgacaccg cctgcaaccg ccggctttcg ccattcaggt ggcgccgcgc
      421 ggccgaaccc gtgagcgcct cgatgcggcg cacgccggcg ctcaccgcgc cttccgccac
      481 gatggcgatc agcccgatgt caccggtacg ctgcacgtgg gtgccgccgc acagctcgac
      541 cgaataggcc ttgcccgcag gctcgccctc ttcatatcgg cccatcgaca ccacgcgcac
      601 ctcatcgccg tacttttcgc cgaacagggc gcgcgcgccg ctggcaatcg cctcgtccac
      661 ggccatcaac cgcgtcacca ccggcgcatt ctgcaacacc agcgcgtttg ccagatcttc
      721 cacctgtccg atttcgtcat cgttgaccgg cttgggatgg ctgatgtcga agcgcagccg
      781 ttccgggctg accagagacc ccttctgcgc cacgtgatcg ccaagcacat gccgcaacgc
      841 ctcgtgcagc aagtgcgtcg ccgagtggtt ggcgcggatg gcggaccggc gccgcgtgtc
      901 gaccgcaagg tttagcgccg cgccaacctt gagcacccct tcctcgaccc ggacggcatg
      961 cgcgaaaacc tctcccagcc gcttctgcgt gtcgttcacc gacaaccgca cgccctcgcc
     1021 cgtcatggcg ccggcatcgc caacctggcc gccggactcg ccataaaatg gcgtctggtt
     1081 cagcacgaca aagccggtct cgccggcctt caaagcgcta acttcggcac catcccggat
     1141 cagcgccgtg acgacgccct cggccgcctc cgtgtcgtag cccaggaact ccgtcgcgcc
     1201 ggcccgctcc ttgatcgcga accagaccgc ttccgtcgcc gcctcgcccg atcccgccca
     1261 ggccgcgcgc gcctcggcct tctgccgcgc catggccgcg ttgaacccat cgacatcaac
     1321 gccgatgccg cgcgcccgca gcgcatcctg cgtcagatcg agcggaaatc cataagtgtc
     1381 ataaaggcgg aacgccgtct cgcccttcag cgtcgcgccg ctggtgaggc cggccgcctc
     1441 atcctcgagc agcgacaggc cgcgctccag cgtccgccgg aaccgcccct cctcaagctt
     1501 cagcgtctcg gcgatcagcg cctccgcccg gtgcaactcg ggatacgcct gtcccatctc
     1561 gcgcaccagc gtgtgcacca gacggtgcat cagcggctca cgcgcgccca ggagctgcgc
     1621 atgccgcatg gcgcggcgca tgattcggcg cagcacatag ccacgcccct cgttggacgg
     1681 cagcacgccg tccgcgacca gaaaggcaga ggcccgcaga tggtccgcga tcacgcggtg
     1741 gctcgccttc tgcggcccct ccgcgtccac cgacaccaga tcggcgacgg tgcggatgat
     1801 cgaccgcatc atgtcggtct cgtaattgtc atgcgtgccc tgcaagactg cggatatgcg
     1861 ctccagtccc atgccggtgt cgatcgacgg cttgggcagg cgcacacgct ctccgccggg
     1921 cagctgctcg aactgcatga acacgaggtt ccagacctcg atgaaccggt cgccgtcggc
     1981 atcggccgaa cccggaggcc cgccgggaat atgcggtccg tggtcgtaga aaatttccga
     2041 gcatggccca caaggcccgg tgtcacccat ggcccagaaa ttgtccgacg tcgggatgcg
     2101 gatgatgcgc tcgtccggca ggcctgcgat cttcttccac agaccgaagg cctcgtcgtc
     2161 ctcatggtag accgtgacca tgagcttgtc cttcggcagg ccatactctc gcgtcaccag
     2221 cgtccaagcg agttcgattg cccggtcctt gaaatagtca ccgaacgaga agttccccag
     2281 catctcgaag aacgtatggt gccgcgcggt gtagccgacg ttgtccaggt cgttgtgctt
     2341 gccgccggcc cgcacgcatt tctgcgatgt cacggcccgg ctgtagggtc ggtgctcctg
     2401 accggtgaag acattcttga actgcaccat ccccgcgttg gtgaacatca gcgtgggatc
     2461 gttgcgcgga accagcggcg acgaggcaac gacctcgtgt ccgttcttcg cgaagaaatc
     2521 gagaaaggtg gagcggattt cgttgacgcc actcatgatg aacgaccgtg cgaccctgaa
     2581 agcagggggc cgccggcctg atgcatcctg ctccgcccgc cgccccgaat ggaaccgcag
     2641 cccttgtaga gcctgagcgc cgaaaggcaa acccggaatt cagcatccgg ccggcgcgac
     2701 gcccgatctt ccgccgcctc agtgctcggg cggaccatcg tcctccggct ccgcaccgcc
     2761 attctccagg atgcgctcgg cgatcagccc cgcgttctgc ctgatcatct gctcgatgcg
     2821 cccggcgatc tcggcgttct gccgcaggaa cgccttggcg ttctcccgcc cctgccccaa
     2881 acgctggctg tcgaaagaga accacgcccc ggacttctcg acgatgccgg ccttgacccc
     2941 gagatcgacc agctcaccca tcttggagac gccctcgcca tacatgatgt cgaactcgac
     3001 ctgcttgaac ggcggcgcca ccttgttctt caccaccttg acgcgcgtgg tgttgccgac
     3061 gacctcctcg cgctccttga tcgcgccgat gcggcggatg tcgaggcgaa ccgaggcata
     3121 gaatttcagc gcgttgccgc ccgtcgtcgt ctccgggctg ccatacatca cgccgatctt
     3181 catgcggatc tggttgatga agatcaccat cgtgttggag cgcgagatcg aggcggtcag
     3241 cttgcgcagg gcctggctca tcagccgcgc ctgcaggccc ggctggctct cgcccatctc
     3301 gccctcgatc tcggccttcg gcgtcagcgc cgcgaccgaa tcgaccacga gcacgtcgac
     3361 cgcgccggag cgcaccagcg tatcggtgat ctccagtgcc tgctcgccgg tgtccggctg
     3421 cgaaatcagc aggttttcaa ggtccacgcc gagcttgcgg gcatagaccg gatcgagcgc
     3481 atgctccgcg tcgatgaagc cgcagatgcc gcccttcttc tgggcttccg cgatggtgtg
     3541 cagcgccagc gtggtcttgc ccgacgattc cggcccgtag atctcgatca cgcggccgcg
     3601 tggcaacccg ccgacgccga gcgcgatgtc gaggccgagc gaccccgtcg acaccgtctc
     3661 gatctcgatg gccttctggt tcttgcccag ccgcatgatc gagcccttgc cgaaggcgcg
     3721 ttcgatctgg ctgagggcgg cgtcaagcgc cttggtcttg tccatcgagg atccttcaac
     3781 gagtcgcagc gcgggctgag tcatggtgtc gtccccttat gccgggcgca ggcgcgccgt
     3841 tcaatgggga atatgtgcat gatttgttct taaagaaaag ttcttttttt gttcttgttg
     3901 acataatttg ttctggccat cgatgccggc ctgatcctcg cccggcgtct tgacgagacc
     3961 ggccgcggtc gcatcggaca gctggctgac gagcagggtt tcgaactcgg gtcacgagcc
     4021 ggttcaggac ggctgcgcct gcgtcaaggc ggcgaaatcc ctgctcggcc ggaattgtcg
     4081 aatgtactcc caaagcggga attgattcct gcgtttcttc ctcctagacg tcagaggttg
     4141 taatcaacat tggagagggg catgcgtttt ggtcgttttg caatggcagg atgtgtcgcc
     4201 atggggttcg cagtgtcgag cggggttgct tccgcaggcg aacgcgtgct gtcgacggtc
     4261 ggcagctggc gcgtcatact caacctcaat ccggacaaca gcttcaaccg ctgcctcatc
     4321 gaaaccaccc agggtggcca gatgctacgg attgccggcg gcgcgaagag cacggatctg
     4381 tccctgtcgg tgccggccat gggcattccg aacggcggtc agggcaccat tggttccgac
     4441 ggcatgcaac cggagtattt cacgttcgca gccaacaagc tgcgcgcctg gacagcgatc
     4501 ccgtgggaaa tgggtttgca cctcgagaag gcgcgctcga tcgaggtcgg ggtcaaccgc
     4561 cggcaaatga agtggcgcgt gagcgggacg aaacaagcga tgattcgcat gacggactgc
     4621 gtcaatcaga acgcgccgta agcgctgtgg tcattgcttc ggactcccgg gagttccggg
     4681 tgtccggggc gatcacccct ccatcaccgt cttcaccttc tcgatcagct gcttgagcga
     4741 gaacggcttg ggcaggaaat cgaactgctg gccttccggc aggttcttgc ggaaggcttc
     4801 ctcggcatag cccgagacga agatcacttt caggtccggc gcgagcttgc gcaattcgcc
     4861 cagcatggtc ggcccgtcca tttccggcat caccacgtcg gaaaccacga gatcgatgtc
     4921 cttcagccgg tccttggcga gttccagccc gtcgaggccg gagggcgcct gcaacacggt
     4981 gtagccgcgc gaggagagcg cgcgggcggc gaagttgcgc accgcctcct cgtcctccac
     5041 cagcatgatc gtgcccttgc cggtgaggtc ggcggcgggt gccttggtct cgccggccgg
     5101 gcgct
//
LOCUS       DAOMXN010000185         5079 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_8773_length_5079_cov_11.637142, whole genome shotgun sequence.
ACCESSION   DAOMXN010000185 DAOMXN010000000
VERSION     DAOMXN010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 5079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5079
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8773_length_5079_cov_11.637142"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /gene="ruvB"
                     /locus_tag="PK812_11390"
     CDS             complement(<1..698)
                     /gene="ruvB"
                     /locus_tag="PK812_11390"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HRJ70200.1"
                     /translation="MSRAASKPGLVSGDKRDDDIDASIRPKLLADFTGQAGARQNLKV
                     FIEAAKTRGEALDHVLFVGPPGLGKTTLAQIVARELGVNFRSTSGPVIAKAGDLAGQL
                     TALEERDVLFIDEIHRLNPAVEEILYPAMEDYQLDLMIGEGPAARSVKIDLPKFTLVG
                     ATTRQGLLTTPLRDRFGIPIRLNFYTVEELESIVRRAARVMGIGIVDEGANEIARRAR
                     GTPRIAGRLLRRVR"
     gene            complement(695..1189)
                     /locus_tag="PK812_11395"
     CDS             complement(695..1189)
                     /locus_tag="PK812_11395"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRJ70201.1"
                     /translation="MSEATDRMDLAFRAIVDKAPLVDFVMRRWGTSGMLIGMHRYDVK
                     DLEATGLYLGDELVAFANWTQRDRMILICAVHSLRSGEGFARQLLEELKRLARARGAT
                     VLRAMASNDNLPALAFFQLNGFRLTTLYVGAIDAFRGAMPGLISKGYRGIDVRDAIEL
                     EFLL"
     gene            complement(1186..1803)
                     /gene="ruvA"
                     /locus_tag="PK812_11400"
     CDS             complement(1186..1803)
                     /gene="ruvA"
                     /locus_tag="PK812_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003613123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HRJ70202.1"
                     /translation="MIGKLKGFIDSYGEDCVILDVHGVGYVVFCSTRTLQNLPQPGDA
                     ATLAIETQVREDAIRLFGFRSDAERDWFRLLQSVQGVGSKVALSILSIMAPGELATAI
                     ATQDKAMIGRASGVGPKLAARIIAELKDKAPAFGSVDPALVKLTGDVDDKKAPGPARD
                     AVSALTNLGYGQPQAAAAVAAALKALGEGADASALIRRGLKELAS"
     gene            complement(1800..2183)
                     /locus_tag="PK812_11405"
     CDS             complement(1800..2183)
                     /locus_tag="PK812_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70203.1"
                     /translation="MRKPAAILALLASAASVSAVASPVLSPAGEYALNASDCRARRIF
                     VTIADDSVTFPVFSCKGVSFDFASQDSAGATYKGLAKACIAEGQTQASPRTFRVVRSG
                     STAQFFWADGTRSGRLQHCAERKRR"
     gene            complement(2196..2702)
                     /gene="ruvC"
                     /locus_tag="PK812_11410"
     CDS             complement(2196..2702)
                     /gene="ruvC"
                     /locus_tag="PK812_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HRJ70204.1"
                     /translation="MNAPVRILGIDPGLRRTGWGVIDCEGARVSFVACGSVDPDPKLD
                     LGPRLALLHEGLTRLIADFSPDEAAVEETFVNADSRSALKLGAARGVAMLVPAQAGLV
                     IGEYAPNHVKKTVVGAGHAEKQQIRLMVKVLLPKAVISSDDAGDALAIALCHAQNRGM
                     RALKQRLA"
     gene            2763..3545
                     /locus_tag="PK812_11415"
     CDS             2763..3545
                     /locus_tag="PK812_11415"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRJ70205.1"
                     /translation="MLADFFLWMAGPAPPATVALLSLFALAAGISRGLTGFGAGMIFV
                     PLAGGLVGPQQAVALIWTMDAPNMVPVAFGSARRADWRESLWLFAGWLVSTPVGLWLL
                     TRLDPLTVRWAVCILILCAMTLLIGGWSYRGRPSLALAIATGLVAGITGGLAGLSGPP
                     IVMLWLAAAMATATNVRDNLNVFFLLTTVVNAVLLPAYGILTWPALRLGLLLALPYGL
                     GVYAGTLAFRAVSDRNYRRAAYFVIGGAALLALPLMDRFLGR"
     gene            3584..4012
                     /gene="ybgC"
                     /locus_tag="PK812_11420"
     CDS             3584..4012
                     /gene="ybgC"
                     /locus_tag="PK812_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003613984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HRJ70206.1"
                     /translation="MSEPHAPHELTLRVYYEDTDFSGFVYHARYLHFFERARTEMLRA
                     LGHDQRAMFANPDGPESFVVRRMAIEFLRPALMDDIVCVRSATTRVAGATMQIDQRLW
                     RGGEELCRADVQVAYLVGGKPRRMPDNLRALFEGAAAAAD"
     gene            4268..4975
                     /gene="tolQ"
                     /locus_tag="PK812_11425"
     CDS             4268..4975
                     /gene="tolQ"
                     /locus_tag="PK812_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HRJ70207.1"
                     /translation="MDGGPATELTLFGMFWSAHFVVKAVMLGLLLASVWTWAIIVDKM
                     LLFRRVAKGMDRFEEVFWSGQSLEELFRNLSQRPTSGMATLFIAAMREWKRAFEGSGR
                     GFASLQSRIDKVMDVSIQREVEKLESKLFILATVGAAGPFIGLFGTVWGIMTSFRSIA
                     ASKNTSLAVVAPGIAEALFATAIGLIAAIPAVIAYNKFSKDLARTQNRLEGFADEFSA
                     ILSRQIDERAEPVRNAA"
BASE COUNT          808 a         1717 c         1665 g          889 t
ORIGIN      
        1 tcgcgcacgc gccgcagcag ccggccggct atgcgcggcg taccgcgcgc gcgccgcgca
       61 atctcgttgg cgccttcgtc tacgatgccg atgcccatca cgcgcgcagc gcgcctgacg
      121 atgctttcaa gctcctcgac cgtgtagaaa ttcagccgga tcgggatgcc aaagcggtca
      181 cgcaacgggg tcgtcaacag cccctgccgc gtggtggcgc cgaccagcgt gaacttcggc
      241 aggtcgatct tcaccgagcg cgctgccggc ccctcgccga tcatcaggtc gagctggtag
      301 tcctccatcg cgggatagag gatttcctcc accgccggat tgagccggtg gatctcgtca
      361 atgaacagaa cgtcccgctc ttccagtgcc gtgagctggc ccgcaaggtc gccggccttg
      421 gcgatcacag ggcctgaggt ggaacggaaa ttgacaccga gttcacgcgc cacgatctgc
      481 gccagcgtcg tcttgccgag gcccggcgga ccgacgaaca gcacatggtc cagcgcttcg
      541 ccgcgcgtct tcgccgcctc tatgaacacc ttgagattct ggcgcgcacc ggcctgtccg
      601 gtgaaatcgg ccagcagctt gggacggatc gacgcatcga tgtcgtcgtc ccgcttgtcc
      661 cccgaaacca gaccgggctt ggaagcggct cggctcacaa caggaactcc agttcgattg
      721 cgtcgcgaac gtcgatgccc cggtagcctt tggagatcag cccgggcatg gccccgcgaa
      781 aggcatcgat ggcaccgacg tagagcgtgg tgagacggaa accattcagt tggaagaacg
      841 ccagagccgg cagattgtcg ttggatgcca tggcgcgcag caccgtcgcg ccgcgggcgc
      901 gggcaagccg tttcagttcc tccagaagct gccgggcgaa cccctcacct gaccgcaggg
      961 agtgcacggc gcagatcagg atcatccggt cccgctgggt ccagtttgcg aaagccacga
     1021 gttcgtcacc cagatagagc ccggttgcct cgagatcctt gacgtcatag cgatgcatgc
     1081 cgatgagcat ccccgaagtg ccccagcgcc gcatgacgaa atcgaccagg ggcgccttgt
     1141 ccacgatggc gcgaaaggcc aggtccatgc gatccgtcgc ctcgctcaac tcgccaactc
     1201 cttcaatccc cgccggatca gggcggaggc gtccgctccc tcgcccagtg ccttgagcgc
     1261 ggcggcgacg gcggctgctg cctgcggctg gccatagccc aggttggtca gcgccgagac
     1321 cgcatcgcgc gcagggccgg gcgccttctt atcgtccaca tcgccggtaa gcttgaccag
     1381 cgccggatcg accgagccga aggcgggggc cttgtccttg agttcggcga tgatgcgggc
     1441 ggcgagtttt ggcccgacgc cggaggcccg cccgatcatg gccttgtcct gcgtggcgat
     1501 tgcggtggcg agttcgccgg gcgccatgat cgagaggatc gagagcgcga ccttggagcc
     1561 aacgccctgc acgctctgga gcaggcggaa ccagtcacgc tcggcatccg acctgaagcc
     1621 gaacagacgg atcgcgtcct cgcgcacctg cgtctcgatg gcaagcgtcg cggcgtcgcc
     1681 gggctgcggc agattctgca acgtgcgggt ggagcagaac accacatagc cgacgccatg
     1741 cacgtcgagg atgacgcaat cctccccata gctgtcgatg aagcccttca gcttgccgat
     1801 catcgccgtt tcctttccgc gcaatgctgg agcctgccgc tgcgcgtgcc gtccgcccag
     1861 aagaactggg cggtcgatcc gctcctgacc acccggaacg tgcggggcga ggcctgcgtc
     1921 tgcccttccg cgatacaggc cttcgccagc cccttgtacg ttgcaccggc gctgtcctgc
     1981 gaggcgaaat cgaagctcac gcccttgcag gaaaacaccg ggaaggtgac gctgtcgtcg
     2041 gcgatggtca cgaagatgcg gcgggccctg cagtcgctgg cattgagcgc atactccccg
     2101 gctggcgaca gcaccggtga cgcgacggcg gagacggacg cggccgatgc cagaagggcg
     2161 agaatggctg cgggcttgcg catcaggggt cccgttcagg caaggcgctg cttcagggcg
     2221 cgcatgccgc gattttgcgc gtggcacagg gcgatcgcaa gggcatcgcc cgcatcgtcg
     2281 gaggagatga cggccttggg cagcagcact ttgaccatca gccggatctg ctgcttctcg
     2341 gcgtgacctg cgccaacgac cgtcttcttc acgtggttcg gcgcgtattc gccgatcacg
     2401 aggccggcct gcgccggcac gagcatcgcc acgccgcgcg ccgcgccaag cttgagggcg
     2461 gagcgggagt cggcgttgac gaatgtctcc tccaccgccg cctcgtccgg cgagaaatcc
     2521 gcaatcagcc gcgtcaggcc ctcatgcagc agagccaggc gcgggccgag gtcaagcttc
     2581 ggatcgggat cgacggaccc gcaggcgacg aacgacaccc gtgcgccctc gcagtcgatc
     2641 acgccccacc cggtcctgcg caggccggga tcgatgccga ggatgcgaac gggtgcattc
     2701 atttgcggtg gcgacctgcg aatcgcgcgg atagggtggc gccatcctat cgattcccgg
     2761 acatgcttgc cgattttttc ctctggatgg ccggcccggc accgccggcg accgtagccc
     2821 tgctcagcct gttcgcgctg gctgccggca tcagccgcgg actgacgggt tttggcgcgg
     2881 gcatgatctt cgtgccgctc gccggcggcc tcgtggggcc gcagcaggcg gtcgcgctga
     2941 tctggaccat ggacgccccc aacatggtgc cggtcgcctt cggctcggcg cggcgggccg
     3001 actggcgcga atcgctctgg ctgtttgccg gctggctggt ctccacgccc gtcggactct
     3061 ggctgctgac gcgtctcgat ccgcttaccg tgcgctgggc agtctgcatc ctcatccttt
     3121 gcgccatgac gctgctgatc ggcggctgga gctacagagg caggccaagc cttgccctgg
     3181 cgatcgcgac ggggctcgtc gccggcatca ccggcggact ggccggcctg tccggtccgc
     3241 ccatcgtcat gctctggctc gccgcggcca tggcgacggc caccaacgtg cgcgacaacc
     3301 tgaacgtgtt cttcctgctc acgacggtgg tcaacgcggt gctgctgccg gcctacggca
     3361 tcctgacctg gccggcgctg cgcctcgggc tgctgctggc cctgccctac ggtcttggag
     3421 tctacgccgg cacgctcgcc tttcgcgccg tcagcgatcg caactaccgg cgcgccgcct
     3481 atttcgtcat cggcggggcg gcgttgctcg ccctgccgct gatggaccgt ttcctcggtc
     3541 gctgagtgga cacggccgcc gccttgcggc aagtcttcgc cccatgagcg aaccgcatgc
     3601 gccccacgaa ctgaccctgc gcgtctatta cgaggacacg gatttctccg gtttcgtcta
     3661 tcacgcgcgc tatctccact tcttcgagcg cgcccgcacc gagatgctgc gcgcgctcgg
     3721 tcatgaccag cgggcgatgt tcgccaatcc ggacgggccg gaaagcttcg tggtgcggcg
     3781 catggccatc gaattcctgc gcccggcgct gatggacgac atcgtctgcg tgcgttcggc
     3841 aacgaccagg gtcgccggcg ccaccatgca gatcgaccag cggctctggc gcggcggcga
     3901 ggaactttgc cgggccgacg tgcaggtcgc ctatctcgtc ggcggcaagc cgaggcggat
     3961 gccggacaat ctgcgcgcgc tgttcgaagg agcggccgcg gcggctgact gatttccgcc
     4021 aagctggtct gcgtcctgcg catttgcgtt tggcgcggca atttgtaaac cgcccgttaa
     4081 ccatcgtggc ggattgatcg aaccgggtcc gcgcacgtgg gcccggcgct gcttttcgcc
     4141 cgcccgtgcc caagtttgga cgaaaacagc ggtcaagagc aaaccggcgg gcgcgaatgc
     4201 cgccacgccc ctcagatggc tgtgcggaag ctggccgcgc agcgtcagga gaaccgatat
     4261 gcatcccatg gacggcggac ccgccaccga actcaccctg ttcggcatgt tctggtccgc
     4321 ccatttcgtc gtcaaggccg tgatgctggg gctgcttctg gcctcggtct ggacgtgggc
     4381 gatcatcgtc gacaagatgc tgctgttccg ccgtgtggcg aaaggcatgg accggttcga
     4441 ggaagtgttc tggtccggtc agtcactgga ggagttgttc cgcaatctgt cgcagcgccc
     4501 cacatccggc atggccacgc tgttcatcgc cgcgatgcgc gagtggaagc gcgccttcga
     4561 aggctccggc cgcggctttg cctcgcttca aagccgcatc gacaaggtga tggacgtgtc
     4621 catccagcgc gaggtggaaa agctggaatc gaagctgttc atcctggcga cggtgggcgc
     4681 ggctggaccg ttcatcggcc tgttcggcac ggtctggggc atcatgacct cgttccggtc
     4741 gattgcagcc tccaagaaca cctcgctcgc cgtggtcgcg ccgggcatcg cggaggccct
     4801 gttcgccacc gccatcggcc ttatcgcggc gattcccgcg gtgatcgcct acaacaaatt
     4861 ctccaaggat ctggcgcgga cgcagaaccg gctcgaaggc ttcgccgacg agttctcggc
     4921 catcctgtcg cgacagatcg acgagcgggc cgaacccgtt cgcaacgcgg cgtgatgggt
     4981 gggcgcatga tctgtttcgc caaaccggtc cccacttttg cggatcatgc gcagcgcgcg
     5041 caggtccaag ggagcagcca gccatgggca tgagcacgg
//
LOCUS       DAOMXN010000186         5060 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_8815_length_5060_cov_12.007992, whole genome shotgun sequence.
ACCESSION   DAOMXN010000186 DAOMXN010000000
VERSION     DAOMXN010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 5060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5060
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8815_length_5060_cov_12.007992"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="PK812_11430"
     CDS             <1..439
                     /locus_tag="PK812_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MmcB family DNA repair protein"
                     /protein_id="HRJ70208.1"
                     /translation="QSPTAIRIARGVRRHLFALGFSSVSELPLRSGRRADLVAVNARS
                     EIWIVEIKSSIDDLRADAKWPDYRLHCDRLFFATAPEVPDHIFPPDAGLLIADGYGAS
                     LLREAPHHPLPGATRKEVVARFARHAADRLHLLFDPGLSGRAT"
     gene            570..2156
                     /locus_tag="PK812_11435"
     CDS             570..2156
                     /locus_tag="PK812_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HRJ70209.1"
                     /translation="MQWRVKLAGATLAGSLLFSAAALAQATYHRGNPGDPESLDPHKT
                     STVVESDILLDLYEGLVTYDAKANIVPGVAESWSTSPDGLTYTFKLRANAKWSNGEAV
                     KADDFVFSFRRLLDPATGAKYANILYTLKNAEKVNKGQAKLEELGAKAVDERTLELTL
                     ENAAPYFVAQLAHQTGLPVHPASVGKHGKDFVKAENKVSNGAFMLKSNTPNDKIVVVK
                     NPHYRDAASVKLDQVIFYPIEDRSAGLRRFQAGEVHTYNDVPTDQIKFIRETLKDQFK
                     VAPYLGTYYFAVNTKKAPFSDARVRMALSMVIDREFLAEQIWGGTMVPAYSFVPPGIG
                     NYAEPAFANYKDMSPIEREDKAKALLKEAGYGPGGKKLEIEIRYNTSENHKNTSIAMA
                     DMWKPLNVEVKLVNADIKTHYALLRDKGDYDVARAGWIADYSDPQNFLFLAEGNNQGL
                     NYASWINPEYDGLMKDAAREQDLKKRAEILKKAEAIFMREQPYLSLLYYGSKNLVSPK
                     IQGWEPNVLDRHLSRFLSLK"
     gene            2205..3125
                     /locus_tag="PK812_11440"
     CDS             2205..3125
                     /locus_tag="PK812_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008599179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRJ70210.1"
                     /translation="MGLYVLRRLLAAIPTLFVIVTIAFFLIRVVPGGPFDLERPLDPK
                     VLENLKRVYGLDKPLFEQYLTYLWSLARGDLGPSFVIRDFSVAEVFARGLPVSIRLGL
                     LALLVAVIVGGTLGVIAAFRQNRPADYAVVGLATLGITVPTFVTAPIFQIVFGLMLAW
                     LPVGGWNDGAIRNTILPVLTLSLPQIAVIARMTRASMIETLRANHIRTLRAQGLSTAT
                     IVRHALRSAALPVVSYLGPAAAQLLTGSLVIESIFGLPGVGRYFVEGALNRDYTLVMG
                     TVVMIAVFVLIFNLIVDIAYAVLDPRVRHG"
     gene            3118..4227
                     /locus_tag="PK812_11445"
     CDS             3118..4227
                     /locus_tag="PK812_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRJ70211.1"
                     /translation="MADAVAIAGRGYWASAWLRLKGNRAAVASLVALILLALACLAGP
                     FFTGHAYDRVYQDYVREPASLTVHPTADRIRPALERVASRMRMSLKEAEIGPVSVRAV
                     FASPRAIDERNLAFIERSDLFGPAKVTERRAEGREVAVEVPIERNRFLFGTDANGRDL
                     LTRILHAGRISLAIGLLASGVALIIGVTYGAAAGYAGGRVDMVMMRIVDIIYSLPFIF
                     FVILLVVFFGRNFLLMFLAVGAVEWLDMARIVRGQTLSLKRQEFVQAAEALGVGAGGI
                     LKRHIIPNLIGPVVVYVTLLVPKVILLESFLSFLGLGVQEPMTSLGVLISDGAKAIQG
                     GTYLLIFPAAVLIVMLFALNFIGDGLRDAFDPKDR"
     gene            4227..>5060
                     /locus_tag="PK812_11450"
     CDS             4227..>5060
                     /locus_tag="PK812_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRJ70212.1"
                     /translation="MEGALLDIRGLNVDFRTHDGTVHAVRGVDLAIAPGETLAIVGES
                     GSGKSQIMNAVMGLLAANGAASGSVRFEGAEILGLPPKALNRIRGAKIGMIFQEPMTS
                     LDPLYRISRQMVEPLMRHRGLSGSAARKEAIRLLGLVRIPDPANRIESYPHELSGGQR
                     QRVMIAMAIANDPTLLIADEPTTALDVTVQAQILDLLSDLKSRLGMAIAFISHDLNLV
                     ERFADRVCVMHRGRIVESGPVREVFASPREEYTRELLAAEPSGRKTPPPADAPVLLEG
                     QN"
BASE COUNT          830 a         1664 c         1677 g          889 t
ORIGIN      
        1 gcaatcgccg accgcgatca ggatcgcgcg cggcgtgcgc cggcacttgt tcgcgctggg
       61 gttttcgagc gtgtcggaac tgccgctgcg cagcgggcgg cgcgccgatc tggtggcggt
      121 caatgcccgg tccgaaatct ggatcgtcga aatcaagtcc tccatcgacg atctgcgcgc
      181 cgacgccaag tggcccgatt accggctgca ttgcgaccgg ctgttcttcg ccacggcgcc
      241 ggaggtgccg gaccacatct tcccgccgga tgccgggttg ctgatcgccg acggctatgg
      301 cgcctcgctg ctgcgggagg cgccgcacca tcccctgccc ggagcgacgc gcaaggaagt
      361 ggtggcgcgc tttgcgcggc acgccgcgga ccggctgcac ctgctgttcg accctggcct
      421 cagcggtcgg gcgacctgag cgtgatcacg gtttcctgat agtcccggca tctgcggtgt
      481 tgccggcagc cgcggattgg tcctagtgtt ggactttcga cggcccgtcg catggccgtg
      541 caacgtcata ggaggaaccg atgtccggca tgcagtggcg agtgaaactg gcgggagcga
      601 cattggccgg aagtctgctg ttctccgcag cggctctggc gcaggcgacg tatcaccggg
      661 gcaatccggg ggacccggag tcgctggacc cgcacaagac ctcgacggtt gtcgagagcg
      721 acatcctgct cgacctctac gaaggcctcg tcacctatga cgccaaggcc aatatcgtgc
      781 caggcgtggc ggaaagctgg tccaccagcc cggatggcct gacctacacc ttcaagctgc
      841 gcgccaatgc caagtggtcc aacggcgagg cggtgaaggc cgacgatttc gtgttttcct
      901 tccgccgcct gctcgatccg gcgaccggcg cgaaatacgc caacatcctc tacacgctga
      961 agaacgccga aaaggtcaac aagggccagg cgaagctcga ggagctcggc gccaaggcgg
     1021 tggatgagcg cacgctggaa ctgacgcttg agaacgcggc cccgtatttc gtggcgcagc
     1081 tcgcgcacca gaccggcctg ccggtgcacc cggccagcgt cggcaagcac ggcaaggatt
     1141 tcgtcaaggc ggagaacaag gtctccaacg gcgccttcat gctgaagtcc aacacgccga
     1201 acgacaagat cgtggtggtg aagaacccgc actaccgcga tgccgcctcg gtcaagctgg
     1261 atcaggtgat cttctatccg atcgaggacc ggtcggcggg cctcaggcgc ttccaggccg
     1321 gagaagtcca cacctacaac gatgtgccga cggaccagat caagttcatc cgcgagacgc
     1381 tcaaggacca gttcaaggtc gcgccttacc tcggcaccta ttatttcgcc gtgaacacca
     1441 agaaggcgcc gttctccgac gcgcgcgtgc ggatggcgct gtcgatggtg atcgatcgcg
     1501 agttcctggc cgagcagatc tggggcggca cgatggtgcc ggcctattcc ttcgtgccgc
     1561 cgggcatcgg caactacgcc gaaccggctt tcgccaacta caaggacatg tcgccgattg
     1621 agcgcgagga caaggccaag gcgctgctga aggaggcggg ctacgggccg gggggcaaga
     1681 agctggagat cgaaatccgc tacaacacct cggaaaacca caagaacacg tccatcgcca
     1741 tggcggacat gtggaagccg ctgaacgtcg aggtgaagct ggtcaacgcc gacatcaaga
     1801 cgcattacgc gctgctgcgc gacaagggcg actacgacgt cgcccgtgcc ggctggatcg
     1861 cggactattc cgacccgcag aacttcctgt tcctggcgga aggcaacaac cagggcctca
     1921 actacgccag ctggatcaac ccggaatacg acgggttgat gaaggatgcg gcgagggaac
     1981 aggacctgaa gaagcgcgcc gagatcctga agaaggccga ggcgatcttc atgcgcgagc
     2041 agccctatct ctcgctgctc tattacggct cgaagaacct ggtctcgccg aagatccagg
     2101 gctgggaacc gaacgtgctg gaccgccacc tctcgcgctt cctctcgctg aagtgacagg
     2161 acgcggcgcg gcggcggcgt ccagccgttg ccgcgcggac agccatgggg ctctacgttc
     2221 tccgaaggct gctggcggcg atcccgaccc tgttcgtgat cgtcaccatc gccttcttcc
     2281 tgatcagggt cgtgccaggc gggccgttcg atctcgaacg cccgcttgat cccaaggtcc
     2341 tggagaacct caagcgggtc tacggcctcg acaagccgct gttcgagcaa tacctgacgt
     2401 atttgtggtc gctggcgcgc ggcgacctcg ggccatcctt cgtcatccgc gacttcagcg
     2461 tggccgaggt gtttgcacgc ggcctgccgg tctcgatccg ccttggcctg ctggcgctgc
     2521 tggtggcggt catcgtcggc gggacgctcg gcgtcatcgc cgccttccgc cagaaccggc
     2581 cggcggatta tgccgtcgtc gggcttgcga cgctcggcat cacggtgccg accttcgtca
     2641 ccgcgccgat cttccagatc gtgttcggcc tgatgctggc ctggctgccg gtgggcggat
     2701 ggaacgacgg ggcgatccgc aacaccatcc tgccggtgct gacgctgtcg ctgccgcaga
     2761 tcgccgtgat cgcgcgcatg acccgggcct cgatgatcga gacgctgcgc gccaaccaca
     2821 tccggacgct gcgcgcgcag ggactttcga cggcgaccat cgtccgccac gccctgcgca
     2881 gcgcggcgct gccggtggtg tcctatctcg ggccggcggc ggcgcaactg ctcaccggct
     2941 cgttggtgat cgaatcgatc ttcggcctgc ccggcgtcgg gcgctacttc gtcgagggcg
     3001 ccctcaaccg cgactacacg ctggtgatgg gcacggtggt gatgatcgcg gtcttcgtgc
     3061 tgatcttcaa cctgatcgtc gacatcgcct atgccgtcct cgacccgagg gtgcgtcatg
     3121 gctgacgcgg tcgcgatcgc cggacgtggc tactgggctt ccgcctggct gcgcctcaag
     3181 ggcaaccggg cggcggtggc cagcctggtg gcgctgatcc tgctggcgct cgcctgcctc
     3241 gccggtccgt ttttcaccgg acatgcctac gaccgggtgt atcaggacta tgtgcgcgaa
     3301 cctgccagcc tgaccgtgca tcccacggcg gaccggatca ggccggcgct ggagcgtgtc
     3361 gcctcgcgca tgcgcatgag cctgaaggag gcggagatcg gccccgtgag cgtgagggcg
     3421 gtgtttgcct cgccccgcgc catcgacgag cgcaacctcg ccttcatcga gcgctcggac
     3481 ctgttcgggc cggcgaaagt gacggagcgg cgggcggaag gccgcgaggt tgccgtcgag
     3541 gtgcccatcg agcgcaaccg cttcctgttc ggcacggacg ccaacggccg cgaccttctg
     3601 acccgcatcc tgcatgccgg gcgcatctcg ctcgccatcg gcctgctggc gtccggcgtg
     3661 gcgctgatca tcggcgtcac ctacggagcg gcggcgggct atgccggggg gcgcgtcgac
     3721 atggtgatga tgcgcatcgt cgacatcatc tattcgctgc cgttcatatt cttcgtcatt
     3781 ctgctcgtgg tgttcttcgg gcggaatttc ctgctgatgt tcctggcggt cggcgcggtg
     3841 gaatggctcg acatggcccg catcgtgcgc ggccagacgc tgtcgctgaa gcggcaggag
     3901 ttcgtgcagg cggccgaggc gctgggcgtc ggcgccggcg gcatcctcaa gcggcacatc
     3961 atccccaacc tgatcggccc ggtggtggtc tacgtgaccc tgctggtgcc gaaggtgatc
     4021 ctcttggaaa gcttcctgtc cttcctcggg ctcggcgtgc aggagcccat gaccagtctc
     4081 ggcgtgctga tctcggacgg ggccaaggcg atccagggcg gcacctatct cctgatcttt
     4141 ccggccgccg tgctgatcgt catgctgttc gcgctcaatt tcatcggcga tggcctgcgc
     4201 gacgccttcg atccgaagga tcgctgatgg aaggcgccct gctcgacatc cgcggactga
     4261 acgtcgattt ccgcacccat gacgggacgg tgcacgccgt gcgcggcgtc gatcttgcca
     4321 tcgcgccggg cgagacgctg gcgatcgtcg gcgagtccgg ttccggcaag agccagatca
     4381 tgaatgctgt gatgggcctg ctcgccgcca acggtgcggc ctccggttcg gtgcggttcg
     4441 agggcgcgga aatcctcggc ctgccgccga aggcgctcaa ccgcatccgc ggcgcgaaga
     4501 tcggcatgat cttccaggag ccgatgacct cgctcgatcc gctctaccgc atcagccggc
     4561 agatggtgga gccgctcatg cgccatcgcg ggctttccgg cagcgcggcg cgcaaggagg
     4621 cgatccgcct gctgggcctc gtgcgcatcc ccgatccggc gaaccgcatc gagtcctacc
     4681 cgcacgaact gtccggcgga cagcgccagc gcgtgatgat cgccatggcg atcgccaacg
     4741 atccgacgct gctgatcgcg gacgagccga ccacggcgct ggacgtcacc gtccaggcgc
     4801 agatcctcga tctgctgagc gacctgaagt ccaggctggg catggccatc gccttcatca
     4861 gccacgatct caatctggtc gagcggttcg ctgatcgggt ctgcgtcatg catcgtggga
     4921 gaattgtcga aagtggaccg gtccgggagg ttttcgccag tccgcgcgag gagtacacgc
     4981 gcgaattgct ggcggccgaa ccgtccgggc gcaagacgcc gccgccggcc gacgcgccgg
     5041 tgctgctcga aggccagaac
//
LOCUS       DAOMXN010000187         4947 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_9108_length_4947_cov_11.413532, whole genome shotgun sequence.
ACCESSION   DAOMXN010000187 DAOMXN010000000
VERSION     DAOMXN010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4947
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9108_length_4947_cov_11.413532"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2208)
                     /locus_tag="PK812_11455"
     CDS             complement(<1..2208)
                     /locus_tag="PK812_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012255104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRJ70213.1"
                     /translation="MAMNISAWSIRKPLPGILLFVVLMILGAYSFQSLPVTRFPNIDV
                     PFVNVRVTQSGAAPSELETQVTKRIEDAVANLAGVKHITSAIGDGSSSTTIEFRLEVN
                     TDRAVNDVKDAVARVRADLPRTIDEPIIQRIDIEGLPILSFAVYAPAKTLEEISWFVD
                     DVVIRNLKSVRGVGGVDRVGGVEREIRVLLDPNRLAALGVTAGEVNRQLRATNVDLAG
                     GRGEIAGQEQSIRTLAGATRVEDLAATQITISGNRKVRLSDIGEVVDGAAEQRTFARL
                     DGKPVVAFSVTRGKGASDAEVADLVKVGIARIAADNPDFDLRLIDSSVSYTIGNYRSA
                     MKTLIEGALLAVLVVLIFLRDFRATLIAACAIPLSVFPTFWAMSQLGFSLNLVSLLAM
                     TLATGVLVDDAIVEIENIVRHMRMGKSAYRASLEAADEIGLAVIAISFTIVAVFAPVS
                     FMGGIAGQYFKQFGLSVAIAVFFSLLVARLITPMMAAYFLKDHKHMEEGEGRIMRAYS
                     SLVETSVRHRWITIVIGAALFAASIWSSKLLPSGFLPGEDVARALLALELPPGSRIED
                     TMRVSDDVMRRLKERPEVRSVFVDGGKVISAGAASREVRKAQVVINFVHKNERKKSQK
                     QLEREISDDLKTVPDIRYWFMKDNGMRGVQMVVQGADGDQVSRSAAALASEMKRVPVL
                     NNVTTTASLDRPEIRIRPRADVAADLGVSTDAISEAIRVGTIGDVGPNLARFNA"
     gene            complement(2208..3413)
                     /locus_tag="PK812_11460"
     CDS             complement(2208..3413)
                     /locus_tag="PK812_11460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRJ70214.1"
                     /translation="MIRLSRTFGVASLALSLTVAAHALAEAQAQNAPTPAGPRVTVTK
                     AVRGELVETVAVTGTLVARDEVLIGSEIDGFRLVEILAEEGDRIEKGQVLARLSRETL
                     DAQLAQNDAAIAKTEAAIAQARNQIATAEASLRQSRQALERTSELKKSGFASQAVLDQ
                     QTNAERADAGRLAAAQDWLKVSQADRASMEAHRRELLVRLGRTEVKAPAAGLISRRAA
                     KLGAVASMSAEPMFRLIANGQIELEGEVPELRIGKVQAGQAAVVTSGATSVPGTVRLV
                     SPEVDRVTRQGKVRIAIPDPAGQRIGSFARGLVETRRVHAVSVPQSAVLYGDQGPFVQ
                     VVEKDRIVSTPVKLGIASEQRVEVLSGLKEDAVYVIKAGAFLRDGDAVSPVEPGKPQA
                     DAGQRMQVR"
     gene            complement(3410..4066)
                     /locus_tag="PK812_11465"
     CDS             complement(3410..4066)
                     /locus_tag="PK812_11465"
                     /inference="COORDINATES: protein motif:HMM:NF025343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRJ70215.1"
                     /translation="MPKLSPEIQVQRQEHILDAAELCFARTGFHRTTMQDICRAARVS
                     AGAVYVYFASKEALIEGIVQRDRDSIMQKLNAVADSPDFLEGLRCVMKDCVLEQPAHK
                     SALFLEIGAEATRNPLVSRLLTEFDRALRQKLESLLARARGEGRIAADVDPRSATLVM
                     AMLGDAMMLRRATDPSFDPATIGPVILNVVARLLGLSAIAQPVLAGDVPAQTFAGVAE
                     "
     gene            4342..>4947
                     /locus_tag="PK812_11470"
     CDS             4342..>4947
                     /locus_tag="PK812_11470"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRJ70216.1"
                     /translation="MQISIIVAIASAICLGLLAGFLLDTSLVLPAAITGLVVPCAVLP
                     WMTFHAIDSRRQLNETLTALEGLAKMDTTTHLPNRAAFRESGKAMFDRLKAGSEPLSA
                     VLIDVDHLDQINQTHGYDLGDAVLAHAAELLNLNIQTGVDLAARYGGAEFILLLPQAD
                     EGWASAFAERLRGAFANRALEHKGAYINVSASFGVAALQPGD"
BASE COUNT          871 a         1705 c         1575 g          796 t
ORIGIN      
        1 ggcgttgaag cgcgccaggt tcggcccgac gtcgccgatg gtgccgacgc ggatcgcctc
       61 cgagatggca tcggtcgaga cgccgaggtc ggcggcgaca tccgcgcgcg gacggatgcg
      121 gatttccggc cggtccagcg acgcggtggt cgtgacgttg ttgagcaccg gaacccgctt
      181 catctccgac gccagcgccg ccgccgagcg cgacacctga tcgccgtccg cgccctggac
      241 aaccatctgc acgccgcgca tgccgttgtc cttcatgaac cagtagcgga tgtccggcac
      301 ggtcttcaga tcgtcgctga tctcgcgctc gagctgcttc tgcgacttct tgcgctcgtt
      361 cttgtgcacg aagttgatca ccacctgcgc cttgcggacc tcgcgcgagg cggctccagc
      421 gctgatcacc ttgccgccgt cgacgaagac gctgcggacc tcggggcgct ccttcagccg
      481 gcgcatcacg tcgtcgctga cccgcatcgt atcctcgatg cgcgatccgg gcggcagttc
      541 gagcgccagc agcgcgcgcg ccacgtcctc gcccggcagg aagccggagg gcagcagctt
      601 gctcgaccag atcgaggcgg cgaacagcgc ggcgccgatc acaatggtga tccagcgatg
      661 gcggaccgag gtttcgacga gggaggaata ggcgcgcatg atgcgtccct cgccctcctc
      721 catatgcttg tgatccttca ggaaataggc cgccatcatc ggcgtgatca ggcgcgcgac
      781 caacagcgag aagaacaccg cgatggccac cgacagtccg aactgcttga aatactgccc
      841 ggcgatgccg cccatgaacg acaccggcgc gaacaccgcg acgatggtga aggaaatggc
      901 gatcacggca aggccaatct catccgccgc ttccagcgag gcgcggtagg cagacttgcc
      961 catgcgcatg tggcgcacga tgttctcgat ctcgacgatc gcgtcgtcca ccagcacgcc
     1021 cgtcgccagc gtcatggcca gcaggctgac cagattgagc gagaagccga gctggctcat
     1081 cgcccagaac gtcggaaaca ccgacagggg aatggcgcag gccgcgatca gcgtggcgcg
     1141 gaaatccctg aggaagatca gcaccaccag cacggccagc agcgccccct cgatcagcgt
     1201 cttcatcgcc gagcggtagt tgccgatggt gtaggacacg gaggaatcga tcagcctgag
     1261 atcgaaatcc ggattgtcgg cggcaatcct ggcgatgccg accttcacca gatcggccac
     1321 ctcggcatcg ctcgcgccct tgccgcgcgt gacggagaac gccaccaccg gcttgccgtc
     1381 aagccgcgcg aacgtgcgct gctccgccgc cccgtccacc acctcgccga tatcggacag
     1441 gcgcaccttg cggttccccg agatggtgat ctgcgtcgcc gccagatcct cgaccctcgt
     1501 ggcgccggcc agcgtgcgga tggactgctc ctgccccgcg atctcgccgc gaccgccggc
     1561 cagatcgaca ttggtggccc tcaactggcg attgacctcg cccgccgtga cgccgagcgc
     1621 cgccagccgg ttcggatcga gcagcacgcg gatctcgcgc tccacgccgc cgacacggtc
     1681 tacgccgccc acgccgcgca cggacttcag gttgcggatg acgacgtcgt ccacgaacca
     1741 ggaaatctcc tccagcgtct tggccggcgc atagacggcg aaggacagga tcggcagccc
     1801 ttcgatgtcg atacgctgga tgatcggctc gtcgatggtg cgcggcaggt cggcgcgcac
     1861 gcgggccacc gcatccttga cgtcgttgac ggcacggtcg gtattcacct caagccggaa
     1921 ctcgatggtg gtcgaggacg agccgtcgcc gatcgccgag gtgatatgct tgacgccggc
     1981 gaggttggcg acggcgtcct cgatgcgctt ggtgacctgc gtctccagtt cggaaggcgc
     2041 cgcgccggac tgggtgacgc gcacgttcac gaacggcacg tcgatattgg gaaaacgcgt
     2101 gaccggcagg ctctggaagc tgtaggcccc caggatcatc agcacgacga acagcaggat
     2161 gcccggcagg ggctttcgta tcgaccaggc ggaaatgttc atggccatca gcgcacctgc
     2221 atccgctggc cggcatccgc ctgcggcttg cccggctcaa ccggcgagac cgcgtcgccg
     2281 tcgcgcagga acgccccggc cttgatgacg tagaccgcgt cctccttgag gccggacagc
     2341 acctccaccc gctgttcgga cgctatgccg agcttcaccg gcgtcgagac aatcctatcc
     2401 ttctcgacga cctgaacgaa cgggccctgg tcgccataga gcacggcgga ttgcggcacg
     2461 ctgacggcat gaacccgccg cgtctcgacc agtccgcggg caaacgagcc gatgcgctgg
     2521 ccggcaggat cgggaatggc gatgcgaacc ttgccctgac gcgtcacgcg gtcgacttcc
     2581 ggcgacacca gacgcaccgt tcccggcacg ctggtcgcgc cgctggtgac gaccgccgcc
     2641 tgcccggcct ggaccttgcc gatccgcagt tccggaacct cgccctcaag ctcgatctgg
     2701 ccgttggcga tcagccggaa catcggctcg gcggacatgg acgcgaccgc gcccagcttg
     2761 gccgcccggc gcgagatgag accggcggca ggagctttca cctcggtgcg ccccagacgc
     2821 accagcaatt cccggcgatg cgcctccatc gaggcgcggt cggcctgcga gaccttgagc
     2881 cagtcctgcg ccgcggccag ccgtccggca tcggcccgct cggcgttggt ctgctgatcc
     2941 agcacagcct gcgaggcgaa gccggacttc ttcagttccg atgtccgttc cagcgcctga
     3001 cgggactggc gcaacgaggc ctcggcggtc gcgatctgat tgcgggcctg cgcgatggcg
     3061 gcctccgtct tggcgatggc cgcatcgttc tgcgcaagct gcgcatccag ggtctcgcgc
     3121 gacagccgcg ccagcacctg ccccttttcg atgcggtcgc cttcctccgc gagaatctcg
     3181 acgaggcgaa acccgtcgat ctccgagccg atgagcactt cgtcgcgcgc cacgagcgtg
     3241 ccggtgacgg ccacggtctc gaccagttcg ccgcgcacgg ccttggtcac ggtgacgcgc
     3301 gggccggcgg gagtcggggc gttctgcgcc tgtgcctcgg caagggcatg tgccgcgacg
     3361 gtcaaagaca gcgcgaggct tgcgacacca aaggttcggg acaggcggat cattcggcga
     3421 ctccggcaaa agtctgggcc ggcacatcgc cggcaaggac gggttgggcg atcgcgctca
     3481 agcccaggag cctggcgacc acattgagaa tgaccgggcc gatggtggcc ggatcgaacg
     3541 acggatcggt ggcgcggcgc agcatcatgg catcgcccag catggccatc accagcgtcg
     3601 cggagcgcgg gtccacgtcg gcagcgatgc ggccctcgcc gcgcgcacgc gccagaaggc
     3661 tttcgagctt ctggcgcagg gcgcggtcaa attccgtcag cagccgcgac accagcgggt
     3721 tgcgcgtcgc ttccgcgcca atctcgagaa acagggcgga cttgtgcgcg ggctgttcca
     3781 gcacgcaatc cttcatcacg cagcgcagcc cctccaggaa atccggggaa tcggcaaccg
     3841 cgttgagctt ctgcatgatg gagtcgcggt cgcgctgcac gatgccttcg atcagcgcct
     3901 ccttcgaggc gaaatagaca tagaccgccc cggcgctgac ccgtgccgcg cggcagatgt
     3961 cctgcatggt cgtgcggtgg aagcccgtgc gggcaaagca caattcggcg gcgtcgagaa
     4021 tatgctcctg gcgctgcacc tggatttcgg gggacagttt cggcatggca ccacgaaaca
     4081 aaacgaacga acgttcgttt ttaatgggtc actcattaag aaagtcaatc catcgccgca
     4141 ttaaatcccg ttcacgaaac ctgaagcgga tgttcagcca gcgctcatgc gctttgacta
     4201 aaactcaacg cagttacgca acggaagctg agacactctt gcctatggtc acgcccaatc
     4261 acaagaaacc gatctcctca catgacgggg cgttgcgtgt tttccggaat tcagacctca
     4321 acgcaggcgt ggcaccgcgc cttgcaaatc agcatcatcg tcgcgattgc ctcggccatc
     4381 tgcctgggcc ttctggccgg attcctgctg gacacctccc tggtgctgcc ggcggccatc
     4441 accggcctcg tcgtaccttg cgccgtgctc ccctggatga cgtttcatgc catcgactcc
     4501 cgccggcaac tcaatgagac gctgacggcg ctggaaggcc tagcaaagat ggacacgacc
     4561 acgcacctgc ccaaccgcgc cgccttcagg gaaagcggca aggccatgtt cgacaggctg
     4621 aaggcgggca gcgagccgct ctccgccgtg ctgatcgacg tggaccacct cgaccagatc
     4681 aaccagaccc acggctatga cctcggcgat gccgtgctgg cccatgcggc cgaactgctg
     4741 aacctgaaca tccagaccgg tgtcgacctg gcggcccgct acggcggcgc cgagttcatc
     4801 ctgctgctgc cgcaggcgga cgagggctgg gcctcggcct tcgccgaacg tctgcgcggc
     4861 gccttcgcca accgcgcgct ggagcacaag ggcgcctaca tcaatgtgtc ggcgagcttc
     4921 ggcgtggcgg cgctgcaacc cggcgac
//
LOCUS       DAOMXN010000188         4883 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_9275_length_4883_cov_9.689934, whole genome shotgun sequence.
ACCESSION   DAOMXN010000188 DAOMXN010000000
VERSION     DAOMXN010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4883
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9275_length_4883_cov_9.689934"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1441
                     /locus_tag="PK812_11475"
     CDS             <1..1441
                     /locus_tag="PK812_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRJ70217.1"
                     /translation="AALARVLAPEPDVLLLDEPTNHLDLPAIEWLEGELKRIRSALVL
                     ISHDRRFLETLLRTTIWLDRGRTRRLDDGFARFESWRDALLEQEELEHHKLERKIAME
                     MDWLRYGVTARRKRNVRRLGLLQDMRKAHRDRRGPAGDVRMTVQAGEASGKLVIEASG
                     ISKAFEGPPLVKDFSIRIARGDRVGIVGPNGAGKTTLLNLLTGQLAPDSGTVSIGATV
                     AMMTLDQRRESLDPKATVAETLTGGRSDLVTIDGQARHVVSYMKDFLFTADQARSPLD
                     ALSGGERGRLMLARALARPSNLLVLDEPTNDLDLETLDLLQERLGDYAGTLLLISHDR
                     DFLDRVVTSVVMAEGNGRWVEYAGGYSDMVAQRGAGVQSRPAVKAAAPRGNTSVGATF
                     MPSEPRSAKRRLTMAEMQALKTLPDDMERLNREITMLQDAIANPALYARDASLFTRAT
                     TRLGAAQAELAAAEERWLELELLREETGG"
     gene            complement(1892..2884)
                     /locus_tag="PK812_11480"
     CDS             complement(1892..2884)
                     /locus_tag="PK812_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRJ70218.1"
                     /translation="MNILVIGAAGMVGRKLLERIVKDGSLNHRPVTAMTLADVVEPAV
                     PAGAGWPVTTRAVDIADPAVGPLLVADRPDVIFHLAAIVSGEAEQNFDKGYAINLDGT
                     RHLLDAIRAAHAASGGTYRPRLVFTSSIAVYGAPFPEPIGDEFFHTPLTSYGTQKAIA
                     ELLLSDYTRKGFLDGVGIRLPTICVRPGKPNLAASGFFSNIIREPLAGKPAVLPVSED
                     VLHWHASPRAAVGFLIHAAQLSGELLGARRNLTMPGLAATVGGQIAALERVAGMSAVR
                     LIERRPDPAIARIVAGWPRSFDPTRAISLGFVAESTFDEIIQVHIEDELGGTVG"
     gene            complement(2939..3808)
                     /locus_tag="PK812_11485"
     CDS             complement(2939..3808)
                     /locus_tag="PK812_11485"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HRJ70219.1"
                     /translation="MPTELVLTNGGSSAVLSSRGAELMSWRVAGREMIWNGDARWWPR
                     RAPILFPFNGWLNGHRFRAKGTTYETPVHGFGPGAEFRLDRVDDAAAILRLTDTEQTH
                     AVFPFAFEVTVNARLDPDGMDFQFEVRNPASEVLPFSIGFHPGFVSPFDGGDPDAYEI
                     VFEKAEAFDIPRIAPGGLFTDQPLDGVPMRGARLNVAAGLARQESLCFRHTASRRLEF
                     VAPSGRRLAIESEGFAHWVIWRRPGGAYLCIEGWSGHGDPVGFVSDITEKPGMTLLPP
                     GDVRRYRFRFRFV"
     gene            3980..4567
                     /locus_tag="PK812_11490"
     CDS             3980..4567
                     /locus_tag="PK812_11490"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRJ70220.1"
                     /translation="MSEAPLGPEAAAQTAAILKHWREDVPDDRLGQLVKDATRSFQRA
                     LQIRLVDHAVSFGHWAFLRVLWQGDGITQRALSERAGVMEPTTFAALKAMEQLGYVER
                     RQTEDNRKNIYVHLTATGRALKDKLVPLAVEINEIAVRGVPADDVATTRRTLLSIIEN
                     LAADELACADRERRLPSTRELSSIVAGRPKRPSAG"
     gene            4657..>4883
                     /locus_tag="PK812_11495"
     CDS             4657..>4883
                     /locus_tag="PK812_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70221.1"
                     /translation="MEEIFFGILAIVGFIAFTLLGPIAFFKGRTLARRVDEADLRLKE
                     AETRIALQEARLRDLATGTPRAAVTPADPAPP"
BASE COUNT          929 a         1600 c         1585 g          769 t
ORIGIN      
        1 ggcggcactg gcgcgggtgc ttgcgccgga gccggacgtg ctgctgctcg acgagccgac
       61 caaccacctc gacctgccgg cgatcgagtg gctggaaggc gaactgaagc gcatccggtc
      121 cgccctggtg ctgatcagcc atgaccggcg cttcctcgaa accctcttgc gcaccacgat
      181 ctggctggat cgcggccgca cccgccggct cgacgacgga ttcgcccggt tcgaatcctg
      241 gcgcgacgcg ctgctcgagc aggaggaact ggagcatcac aagctcgagc gcaagatcgc
      301 catggagatg gactggctgc gctatggcgt caccgcgcga cgcaagcgca acgtccggcg
      361 cctcggcctg cttcaggaca tgcgcaaggc gcacagggac cggcgcgggc cggccggcga
      421 cgtcaggatg acggtccagg cgggggaagc ctccggcaag ctggtgatcg aggcctccgg
      481 catttccaag gccttcgagg gcccaccgct ggtcaaggac ttttccatcc gcatcgcgcg
      541 cggcgaccgg gtggggattg tcgggccgaa cggagcgggc aagacgacgc tgctcaacct
      601 tctcaccggg cagctcgcgc cggattccgg gacagtcagc atcggggcca ccgtggcgat
      661 gatgaccctg gatcagcgcc gcgaaagcct cgatccgaag gcgacggtcg ccgagaccct
      721 caccggcggg cgaagcgacc tcgtcaccat cgacgggcag gcccgacatg tcgtcagcta
      781 catgaaggat ttcctgttca cggcggatca ggcgcggtct ccgctcgacg cgctgtccgg
      841 cggcgagcgc ggcaggctga tgctcgctcg cgcgctcgcc cgcccgtcca acctgctcgt
      901 gctcgacgaa ccgaccaacg atcttgacct tgaaacgctc gatctcctgc aggaacggct
      961 cggcgactat gccggcacac tgctgctgat cagccatgat cgcgatttcc tcgatcgggt
     1021 cgtcacctcc gttgtcatgg cggagggcaa tggccgctgg gtcgagtatg ccggcggcta
     1081 ctccgacatg gtggcgcagc gcggcgctgg cgtgcagtcg cgaccagcgg tgaaggcggc
     1141 ggcgccgcgt ggcaacacca gtgtcggcgc cacgttcatg cccagtgagc cgcgcagcgc
     1201 gaagcggcgg ctgacgatgg ccgagatgca ggccctgaag accctgccgg acgacatgga
     1261 gcggctgaat cgtgaaatca cgatgcttca ggacgccatc gccaacccgg cgctttatgc
     1321 cagggatgca tccctcttca cgcgggcgac gacccgcctc ggtgccgcgc aggcagaact
     1381 ggcggcggcc gaggaacgct ggctggaact ggagctgctg cgcgaagaaa ccggcggctg
     1441 acgccagtca gggcgccggc cgaatgagcg tcagctggcg caccgggatg gtccggtcga
     1501 agagacttgc gctgaccgtg tcgccgtcct ggcatatcag gacgccttcc ccgccttgcc
     1561 accagagcag cgtggtctgg cgatccggag aaacctgacg ctgcgaaaca ttgagtgccc
     1621 gtccgtcaac ctcaagactg aggccaccag acactgcaac cgatacgcgc acgcgaacgg
     1681 cggattccgg gggagaaatc caactaccgc cacgcatatg caaacagtca tctgcaaatg
     1741 cttcgcttcc taatgaaaag aggaaacaaa cggtactcaa cagagatcgc atgtcagtca
     1801 gtatctcgct gctgcgttgt ttggcgcagc ttgtctaata taacttacat ttgcgtcaac
     1861 tcccgggatg tgcgctagcg cacagacacg atcagccgac cgtgcctcca agttcgtcct
     1921 cgatatgcac ctgaatgatc tcgtcaaatg tgctttccgc tacgaaaccc agggaaattg
     1981 cgcgggttgg atcaaaactt cgtggccaac ccgcaactat cctggctatt gccgggtccg
     2041 ggcggcgttc gatcaggcgc acggcactca ttccggccac ccgttcgagc gccgcaatct
     2101 ggccaccaac ggttgcagcc agccccggca tggtcaggtt gcgccgcgct ccaagcagct
     2161 cgccggagag ctgcgccgca tgaatcagga acccgaccgc ggcgcgcggg ctggcatgcc
     2221 agtgcaacac gtcctcgctc accgggagca ccgccggctt gccggcgagc ggctcccgga
     2281 tgatgttgga aaagaagccg gacgctgcga ggttcggctt gcccgggcgc acacagatgg
     2341 tcggcagacg gatgccgacg ccgtccagaa agcccttgcg ggtgtaatcg gacagcagca
     2401 gttcggcgat ggccttctgc gtcccgtagc tggtcaacgg cgtatgaaag aactcgtcgc
     2461 cgatcggctc cgggaaaggt gcgccataca cggcgatcga cgaggtgaag acgaggcgag
     2521 gccgataggt tccgccagaa gccgcatggg cagcgcgaat ggcatcgagc aggtggcgcg
     2581 tgccatccag gttgatggcg tagcccttgt caaaattctg ctcagcctca ccggagacga
     2641 tcgccgcgag gtggaagatc acatccggcc gatccgcgac gagcagtgga ccgacagccg
     2701 gatcggcaat atcgacggcg cgcgtcgtca cgggccagcc ggcgccggcg gggacggctg
     2761 gctcgaccac atcggccagc gtcatggccg tcacaggccg atggttcaat gatccgtcct
     2821 tgacgatgcg ctcaaggagt ttgcgaccga ccatgccggc ggcgccgatg acaaggatgt
     2881 tcacgtgtga cgctccttca caatggcccg gcctggcgac cggtccgggc cgggaaattc
     2941 agacaaaacg aaagcggaag cgatagcgcc tgacatcgcc gggcggtagc agggtcatgc
     3001 cgggcttctc ggtgatatcc gagacaaatc cgacgggatc tccgtgcccg ctccacccct
     3061 cgatgcagag ataggcaccg ccaggcctgc gccagatcac ccaatgcgcg aagccctcgc
     3121 tctcgatagc cagcctgcgc ccgctcggag ccacgaattc gaggcggcgg ctggcggtat
     3181 ggcgaaagca gagcgattcc tgccgagcca ggccggcagc cacgttcagg cgcgcgccac
     3241 gcatcggcac gccatcgagt ggctgatccg tgaacagccc gccgggggca atccgtggga
     3301 tgtcgaacgc ctccgccttt tcgaagacga tctcataggc gtcgggatca cccccgtcga
     3361 agggcgagac gaatccggga tggaagccga tggaaaaggg tagcacttcg ctcgccggat
     3421 tgcggacctc gaactggaaa tccatgccgt ccggatcaag cctagcgttc acggtcactt
     3481 cgaaggcgaa tgggaaaacc gcatgggtct gctccgtatc ggtcagccgc agaatggcgg
     3541 ccgcgtcatc cacgcggtcc agccggaatt ccgcacccgg accgaacccg tgaacaggcg
     3601 tctcgtaggt ggttcccttc gcccggaacc gatggccgtt cagccagccg ttgaagggga
     3661 acaagatcgg cgcccggcgt ggccaccagc gcgcatcgcc gttccagatc atctcgcgac
     3721 cggccacgcg ccaggacatc agttcggcgc cgcgactcga gagtacggcg ctgctgccgc
     3781 cgttggtcag gacaagttcg gtcggcatgg cgcttccgtt tcacatggcg ggattctcga
     3841 ccgcactgtg gcaaaacgct tgtgaccgat gcaagcgaaa ccacgcaacc cgtcgccaca
     3901 cggttgaaac cgacgtgaat ttcgttatgg ctccgttgtc tgccgggagc gcgtgttgaa
     3961 gaaggctgga aaaccacctg tgtccgaagc accgctcggc cccgaggccg ccgcgcagac
     4021 tgcggccata ctgaagcact ggcgcgagga tgtgcccgac gacaggcttg ggcaactggt
     4081 caaggacgcc acgcggagct ttcagcgggc gctccagatc cggctggtgg accatgccgt
     4141 ctcgttcggg cactgggctt tcctgcgcgt gctctggcag ggcgacggca tcacccagcg
     4201 cgcgctcagc gaacgcgccg gagtcatgga gccgaccacg ttcgccgcgc tcaaggccat
     4261 ggaacagctc ggctatgtcg agcggcgcca gaccgaggac aaccgcaaga acatctacgt
     4321 ccatctcacc gcgaccggcc gggcgctgaa ggacaagctg gtgccgctcg ccgtggagat
     4381 caacgagatc gcggtgcgtg gcgtgccggc ggacgatgtg gccacgacac gccgtaccct
     4441 gctgtcgatc atcgagaacc tggccgcaga cgaactggcc tgtgccgaca gggaaaggcg
     4501 cctgccctcg acgcgcgaac tgtccagcat cgtcgcgggc cgaccgaaac gtccttccgc
     4561 cggatagttc caaccctctg cggctggaac aaaacgcact tcatcggata gcgtgacggc
     4621 gcatgctcga gtctcgcgcc cctgcggaga gtgacgatgg aagagatttt cttcggcatc
     4681 ctggcgattg tcggcttcat cgccttcacg ctgcttggcc caatcgcgtt cttcaaggga
     4741 cggacgcttg ctcgccgcgt cgacgaggcc gacctgcgcc tcaaggaagc ggagacgcgc
     4801 atcgccctcc aggaagcgcg gttgcgcgac ctggcgaccg gcactccgag ggcagccgtg
     4861 acgcctgccg atccggcgcc ccc
//
LOCUS       DAOMXN010000189         4858 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_9331_length_4858_cov_11.528420, whole genome shotgun sequence.
ACCESSION   DAOMXN010000189 DAOMXN010000000
VERSION     DAOMXN010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9331_length_4858_cov_11.528420"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..649)
                     /locus_tag="PK812_11500"
     CDS             complement(197..649)
                     /locus_tag="PK812_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HRJ70222.1"
                     /translation="MAMTMNGSFDLPASREVVWQKLNDPDVLKACIPGCQSLERTSDN
                     GFAAVAKLKIGPVSATFRGKVQLSDIDPPNGYRIAGEGEGGIAGFAKGGAGVKLDAIQ
                     DGTRLTYDVEANVGGKLAQLGSRLINSVAKKLADQFFAKFATAVKDQV"
     gene            747..2084
                     /gene="pcaB"
                     /locus_tag="PK812_11505"
     CDS             747..2084
                     /gene="pcaB"
                     /locus_tag="PK812_11505"
                     /EC_number="5.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047472 - 3-carboxy-cis,cis-muconate
                     cycloisomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxy-cis,cis-muconate cycloisomerase"
                     /protein_id="HRJ70223.1"
                     /translation="MTGSGSLYGELFEDHEMLTLFSDHATLQGLLDFEAALSSAQAAE
                     VIIPHNALAPILAACRAELYDTAGIGREATLAGNPAIPMVKRLTEEVARRDLEAARWV
                     HWGATSQDAIDSGRAAQVRSALELLVGRALRLTRALAARTQADRDTVMAGRTLLQHAL
                     PMTFGLKTAYWLDALIGHVEALRAVVPTSPVPLGGAAGTLASLAGKGAALQQRLTGAA
                     APVPFHTGRQSFVRIGCELGMLTGTLGKIARDIMLLMQTEVGEAFEPAASGKGGSSTL
                     PHKRNPVQSLAISAIALRTPGLVSTLLSAMVQEHERAAGGWHAEWEVLPELCILAGAA
                     LSHAIPLIEGLEIDAARMRSNLDLTQGLVLSERVALALGERIGRAEAKAVLAEASRSA
                     LARGLHLRAVLAEDPRVAGQIDPASLDAMFDPSTYLGATHAIIDRVLARCREL"
     gene            2180..2956
                     /gene="pcaD"
                     /locus_tag="PK812_11510"
     CDS             2180..2956
                     /gene="pcaD"
                     /locus_tag="PK812_11510"
                     /EC_number="3.1.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047570 - 3-oxoadipate enol-lactonase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipate enol-lactonase"
                     /protein_id="HRJ70224.1"
                     /translation="MPILNIAGEPFHYRLDGPENAPVLMLSNSLGTDLGMWDQQVEAW
                     SKTYRVLRYDSRGHGRSVVSDGPYSIARLGQDALAIMMELGVKKTHWCGLSKGGMVGQ
                     WLLTHAPERIGRAVLANTACVMGPPGLWNDRIRTVMSRGMGPVADALADRWFTRKFQA
                     AHPKTVAKFVETMRAQNPKGYAGCCAAIRDMDQSETIRSVRNPVLVIAGSADPATPPA
                     RAQAIAERIKGAKLVTLDAAHLSNVEQPQAFARAVQDFLA"
     gene            2953..3339
                     /gene="pcaC"
                     /locus_tag="PK812_11515"
     CDS             2953..3339
                     /gene="pcaC"
                     /locus_tag="PK812_11515"
                     /EC_number="4.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047575 - 4-carboxymuconolactone
                     decarboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxymuconolactone decarboxylase"
                     /protein_id="HRJ70225.1"
                     /translation="MSDDKTRYDAGMKVRRSVLGDAWVDRANAKLNDFNGEFQNFITR
                     YAWGEIWTRPGMERKTRSCCVLSMMIALGNWDEFRLHLRAAFNNGLTRDDIKEVILMA
                     AVYAGVPKANHAVKEAEAVFREMETP"
     gene            3336..3767
                     /locus_tag="PK812_11520"
     CDS             3336..3767
                     /locus_tag="PK812_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRJ70226.1"
                     /translation="MTDSAGAPSGAPVAASQPGVEALIDAYCLAWHHPDPAERRSLIE
                     AALEPDCLYEDPTARVEGSEALLTHIGALLAKRPGFALERLGRADAHHDMLRFGWRMR
                     LADGRTGPDCVDFCRLSMNGKLALVVGFFGPLPSDQDRSAG"
     gene            complement(3775..4242)
                     /locus_tag="PK812_11525"
     CDS             complement(3775..4242)
                     /locus_tag="PK812_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014131200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRJ70227.1"
                     /translation="MDAIDRSILSRVQSDSSRSLSDLAEAVGLSPSACHRRLKALEDA
                     GTIAGYSARLDRTAIGLKIELFVSVSLISQSNESLEAFEKAVQGVPEILACHLLAGRA
                     DYLMHLAVADMADFERIHRNRLSRLPGVSTMTTSFSLRTVKEFRGYPVAGGRG"
     gene            4375..>4858
                     /locus_tag="PK812_11530"
     CDS             4375..>4858
                     /locus_tag="PK812_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HRJ70228.1"
                     /translation="MLVGVPKEVKNHEYRVGLTPESVAEMAHHGHTVLVETRAGAGIG
                     ATDKAYRAAGAIVIDTAAEIFARADMIVKVKEPQDFERKMLRKGQILYTYLHLAPDPE
                     QTADLVKSGAVCIAYETVTDDAGGLPLLKPMSQVAGRMAVHAGASALEKSRGGRGVLI
                     G"
BASE COUNT          840 a         1662 c         1557 g          799 t
ORIGIN      
        1 gccccaccgc cggaacctga aaagaacccc gccgctcgcc ccagcggcgg ggtttctgca
       61 tctgctttgg gtatcggcgt cgggccgatg atcgaggagg aagcagccag ctatcgccga
      121 cgcatcggcc acacccggga ctgacgcccc gtgttccgct ccgtctgcga acgaccgccc
      181 gaaggctttg gcccgctcac acctggtcct tcaccgccgt agcgaacttg gcgaagaact
      241 ggtccgccag cttcttcgcc acactgttga tcaggcgtga gccaagctgc gccagtttcc
      301 cgccgacatt ggcttcgaca tcataggtca ggcgggtacc gtcctgaatg gcgtcgagct
      361 tcacccccgc gccgcccttg gcgaagccgg cgattccccc ttcgccttcg ccagcaattc
      421 gatagccgtt gggcggatca atgtcgctga gctgaacctt gccccggaag gtagcactca
      481 ccgggccgat cttaagcttt gccacggcgg cgaagccgtt gtccgacgtc ctttcgaggc
      541 tctggcagcc gggaatgcag gctttcagca cgtccggatc gttcagcttc tgccagacga
      601 cctcccggga cgccggaagg tcgaaggagc cgttcattgt catagccatg gggcgcttcc
      661 actggtctgc cggcccattg cggccgatca tatcgccgct actgtgccga cagatcggcg
      721 aaaggcaagc cgtaggggaa tcgataatga ccggcagtgg cagtctctat ggcgagttgt
      781 tcgaagacca tgagatgctg accctgttca gcgaccatgc cacgctgcag ggcttgctcg
      841 acttcgaagc cgcgctgtcg agcgcacagg cggccgaggt catcatcccg cacaatgccc
      901 tcgcgccgat actcgcggca tgccgtgccg aactgtacga taccgctggg atcggccgcg
      961 aggcgacgct tgccggcaac cctgcgatcc cgatggtgaa gcggctgacc gaggaggtgg
     1021 cgcggcgcga tctggaggcc gcgcgctggg tccactgggg cgccaccagt caggacgcca
     1081 tcgattccgg ccgcgctgcg caggtccgct ccgcgctcga actgctcgtg ggtcgtgcgc
     1141 tcagattgac ccgggcgctc gccgccagaa cccaggccga tcgcgacacg gtgatggccg
     1201 ggcgcacgct cttgcagcac gccctgccga tgaccttcgg cctgaagaca gcgtactggc
     1261 tcgatgcgct gatcgggcat gtcgaagcgc tgcgcgccgt cgtcccgaca tccccggttc
     1321 cgctcggcgg cgcggccggt acgctggctt ccctcgcagg caagggcgca gccctccagc
     1381 agcgcctgac tggcgccgcc gcgccagtgc ctttccatac cggccgccag agtttcgtac
     1441 gcatcggctg cgaacttggc atgctcacag gaacgctcgg caagatcgcg cgcgacatca
     1501 tgctgttgat gcaaaccgag gtcggggaag cgttcgagcc tgccgcatcc ggcaaggggg
     1561 gctcgtccac cctgccccac aagcgcaacc cggtgcagtc gctggcaatc tcggccatag
     1621 ccctgcgcac gcccggactg gtctcgaccc tgctatccgc gatggtgcag gagcacgagc
     1681 gcgccgccgg cggctggcat gcggaatggg aagtgctgcc cgagctctgc attctcgccg
     1741 gcgccgccct gagccatgcc atcccgctca tcgaagggct ggagatcgat gccgcgcgga
     1801 tgcgatcaaa tctcgacctg acgcagggcc tcgtgctctc tgagcgcgtg gcgctggcgc
     1861 ttggcgaaag gatcggccgg gcggaagcga aggccgtgct tgccgaagcc agtcgctcgg
     1921 cgctcgcgcg cggtctgcat ctcagggccg ttctcgccga agacccgcgc gtcgccggcc
     1981 agatcgaccc ggcaagtctc gacgccatgt tcgacccgtc gacctacctc ggcgcgacgc
     2041 acgccatcat tgaccgcgtc ctcgcgcgct gccgggagct gtgaggccgt cggcatgcgg
     2101 cggaacggcc gctggcgaat cctgccgttc cggtcctatg atgacgcgct gatggcaatg
     2161 acctgagaga gtgtgccgca tgcccatcct gaacatcgcc ggcgaaccct tccactatcg
     2221 actcgacggc cctgagaacg cccccgtcct gatgctgtcc aactcgctgg gcacggacct
     2281 cggcatgtgg gaccagcagg tcgaggcgtg gtcgaagacc taccgcgtgc tgcgctacga
     2341 ttcgcgcggc cacggtcgga gcgttgtcag cgacggcccc tattccatcg cccggctcgg
     2401 ccaggacgcg ctggccatca tgatggaact tggcgtcaag aaaacgcatt ggtgcgggct
     2461 gtccaagggc ggcatggtcg ggcagtggct cctgacgcat gcgccggagc ggatcggccg
     2521 cgcggtgctt gccaacacgg cctgcgtcat ggggccgccc ggcctttgga acgatcgcat
     2581 ccgcaccgtg atgtccaggg gcatggggcc ggtcgccgat gcgctggccg accgctggtt
     2641 cacccggaag ttccaggccg cgcatcccaa gaccgtggcg aagttcgtcg agaccatgcg
     2701 cgcccagaat ccgaagggct atgccggctg ctgcgccgcc atccgcgaca tggaccagag
     2761 cgagacgatc cgctcggtcc gcaacccggt tctggtgatt gccggctcgg ccgatccggc
     2821 gacgccgccc gccagggcgc aggccatcgc cgagcgcatc aagggggcga aactcgtcac
     2881 gctcgacgcg gcccaccttt ccaacgtcga gcaaccgcag gccttcgcca gggccgtgca
     2941 ggacttcctc gcatgagcga cgacaagacc cgctacgacg ccggcatgaa ggtacgccgc
     3001 tccgtgctcg gcgacgcctg ggtggaccgc gccaacgcga agctcaacga tttcaatggc
     3061 gagttccaga atttcatcac gcgctatgcc tggggagaaa tctggacgcg cccggggatg
     3121 gagcgcaaga cgcgctcctg ctgcgtgttg tccatgatga tcgccctcgg caactgggac
     3181 gagttccggc tgcacttgcg cgccgctttc aacaacggcc tgacccggga cgacatcaag
     3241 gaagtcatcc tgatggcggc ggtctatgca ggtgtgccca aggcgaacca cgccgtgaag
     3301 gaggcggagg cagtgttccg cgagatggag accccatgac cgacagcgcc ggtgctccat
     3361 cgggcgcgcc ggtggcagcc tcgcaaccgg gtgtcgaggc gctgatcgac gcctactgcc
     3421 tcgcctggca ccaccccgat ccggcggagc gccgcagcct gatcgaggcc gcgctcgaac
     3481 cggactgcct ctatgaggac ccgaccgcgc gcgtcgaagg cagcgaggcc ttgctgacgc
     3541 atatcggcgc gcttctggcc aaacggcccg gctttgcgct cgaacgcctt ggccgggctg
     3601 acgcacacca cgatatgctc cgctttggct ggcgcatgag actggccgac ggcagaacgg
     3661 ggcctgactg cgtggacttc tgccgtcttt ccatgaacgg caaactggcg ctagtcgtcg
     3721 ggttcttcgg cccactgccg tcagaccagg accggtcagc tggataacgc gcgttcagcc
     3781 ccgccccccc gccaccggat acccccggaa ctccttcacc gtgcgcagcg agaagctcgt
     3841 cgtcatggtc gagacgccgg gcagccgcga cagtctgttg cggtggatgc gttcgaaatc
     3901 cgccatgtca gccaccgcca gatgcatcag atagtcggcg cgcccggcca gcagatgaca
     3961 ggcgaggatt tccggcacgc cctgcaccgc cttttcgaag gcttccagcg actcgttcga
     4021 ctgcgagatc agcgagaccg atacgaacag ctcgatcttc aggccgatgg cggtccggtc
     4081 cagccgcgcc gaatagcctg cgattgtgcc ggcatcctcc agcgccttca gccgccggtg
     4141 acaggctgac ggcgacagtc ccacggcttc cgcgagatcg ctgaggctcc tgctggaatc
     4201 gctttgaaca cgcgacagaa ttgaacgatc aatcgcatcc atgcaaaaat ctcccgacaa
     4261 tcgaattact atcagaattt tctcctgatc cgacagaaaa tttccatgcc gtgcgggagc
     4321 ctttcccgca cgctcctcat taggctgcgc gaaacagcac tgggggaggt agccatgctc
     4381 gtcggcgttc cgaaggaagt gaagaaccac gaataccgcg tcggcctgac acccgagagc
     4441 gtcgccgaaa tggcgcacca cggtcacacc gttctcgtcg agaccagggc cggcgccggc
     4501 attggcgcca cggacaaggc ctaccgcgcg gcgggcgcga tcgtcatcga cacggccgcc
     4561 gagattttcg cccgcgccga catgatcgtg aaggtcaagg agccgcagga cttcgagcgc
     4621 aagatgctgc gcaagggcca gattctctac acctacctgc atctggcccc cgatccggag
     4681 caaaccgccg atctcgtcaa gtcgggcgcg gtctgcatcg cctacgagac cgtcacggat
     4741 gacgcgggcg ggctgcccct cctgaagccg atgagccagg tggccggtcg catggccgtg
     4801 catgccggag cgtcggcgct ggagaagtcg cgcggagggc gcggcgtgct gatcggcg
//
LOCUS       DAOMXN010000190         4847 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_9361_length_4847_cov_9.805718, whole genome shotgun sequence.
ACCESSION   DAOMXN010000190 DAOMXN010000000
VERSION     DAOMXN010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4847
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9361_length_4847_cov_9.805718"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="PK812_11535"
     CDS             <1..181
                     /locus_tag="PK812_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70229.1"
                     /translation="GAPLAQAAAKRVLRQVGALGYEEAILLEAREQEALTRTADCREA
                     VMAFFEKRKAVFHGR"
     gene            complement(182..934)
                     /locus_tag="PK812_11540"
     CDS             complement(182..934)
                     /locus_tag="PK812_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009492493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRJ70230.1"
                     /translation="MHLRFDNRVALVTGGAQGIGRAIAAALRDSGATVHLADIDPAVR
                     DIASGMHMQAHVADIADRSACGATVDAVLARSGRLDVLVNAAGGVRGQVGGPIEAVTE
                     DGWRAIFAANVDGAMWLSQAAAAPMQAARYGRIVNIASGAGLRPSLTGIQAYTAAKHA
                     LVGLTKQLSQDLGPHGITCNAVAPGFVRSNPTTERQWQAYGPEGQARLVAGIHTRRLG
                     KAEDIASAVLFLASDQAEWISGQILSVDGGRS"
     gene            complement(907..1950)
                     /locus_tag="PK812_11545"
     CDS             complement(907..1950)
                     /locus_tag="PK812_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018067650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HRJ70231.1"
                     /translation="MSGAKPILIWGAGAIGGTLGAYWARAGQPVLMTDIVEAHVEACR
                     TQGLHISGPVEDFTQVVPSARIEDVKGVFNVIVLAVKAPATEAACRQLLPHLAPDGFV
                     LSAQNGLNELAIARIVGPERTMGCFVNFGADWLGPGEILFGNRGAVVVGEIDSTIRDR
                     TRRMHELLRLFEPSAVLTDNIWGYLWGKLAYGAMLFATALTTDSMTANFEDPRRFVVF
                     DRLAREVMATAAARGIRPVGFNGFDPACFAPGAPEAGARASIAALAEFNRHTAKTHTG
                     IYRDLAVRRRKTEVDPQIGVIADLAREVGIETPAIRRLVALIHDIEDGRRPMAFETFQ
                     SLIDTCTCASTTA"
     gene            complement(1947..2483)
                     /locus_tag="PK812_11550"
                     /pseudo
     CDS             complement(1947..2483)
                     /locus_tag="PK812_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003524595.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
     gene            2508..3029
                     /locus_tag="PK812_11555"
     CDS             2508..3029
                     /locus_tag="PK812_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009822970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2840 domain-containing protein"
                     /protein_id="HRJ70232.1"
                     /translation="MTGNAAPRVRGGPVPIAQHSDNLTHVELTWVEKKIENWIRFGRE
                     ARETVLDRRRRVLVFRPGSIFAFVRWASNDFGTIISRIDIVRAVKPGEAYQTLPFVRP
                     GGDILLKIEGWPKVQQVLCHIDAVESAGIDVCEVSADHWRHVHHRLIAGHEPRAYTQE
                     RHQAWLKRREIEG"
     gene            3026..3571
                     /gene="traF"
                     /locus_tag="PK812_11560"
     CDS             3026..3571
                     /gene="traF"
                     /locus_tag="PK812_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer signal peptidase TraF"
                     /protein_id="HRJ70233.1"
                     /translation="MTRFGYVMATYFSIMGVAIASFVPVPLRLIWNASASVPIGLYDL
                     DRPGPLHEGDLVAVTPDEPLADFMVARGYIGRGVPLMKHVAALPGQEVCRIGNSVSVD
                     GVPFGEALDRDRRGRPLPVWRGCLRIRDGQVFLMNPSVPDSLDGRYFGPIAARSVIGR
                     ATPLYTDEAGDGRFVWRAATR"
     gene            3640..3987
                     /locus_tag="PK812_11565"
     CDS             3640..3987
                     /locus_tag="PK812_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013368398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF736 domain-containing protein"
                     /protein_id="HRJ70234.1"
                     /translation="MPQIGTFIRDESGFVGHLTTLNLSRDVIIIGAEPSDAENAPDYR
                     VHVFDAMNNETAAEIGAGWKRTGEKAGEYIAILFDDPSFAQPIRANLFRDDDTGRGWS
                     LHWTRAAKRDGKD"
     gene            3992..4720
                     /locus_tag="PK812_11570"
     CDS             3992..4720
                     /locus_tag="PK812_11570"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRJ70235.1"
                     /translation="MRGTVLFLLSGLSFAALLPVGVLAQPASITRPSQGTPYADHIVE
                     AAQRFALPAAWIRAVLRAESAGDSRAISPKGAMGLMQIMPGTWANLRMRLSLGSDPYD
                     PHDNIIAGAAYIRELFDRYGSPGWIAAYNAGPGRYEASLSGRPLPAETRAYVASVSPA
                     LGGGSDSGTTVIAPADPFAWTRAPLFIAQRASTPTADPAPEKRASDDAPGASSSPSLV
                     RDVSAIVPQSNGLFIARADTGTMR"
BASE COUNT          767 a         1670 c         1601 g          809 t
ORIGIN      
        1 gggcgcgccg ctggcgcagg ccgccgccaa gcgcgtgctg cggcaggtcg gtgcacttgg
       61 ctacgaggag gccatcctgc tcgaagccag ggagcaggag gcgctgacgc gcaccgccga
      121 ctgccgggag gcggtcatgg ccttttttga gaagcgcaag gcagttttcc acggccgctg
      181 atcaggagcg tccaccgtcc accgagagaa tctgtccgct gatccattcc gcctggtcgc
      241 ttgccaggaa cagcacggcg ctcgcgatgt cctcggcctt gccgaggcgc cgggtgtgaa
      301 tgcccgcgac gagccgcgcc tggccttcag ggccgtaggc ctgccattgc cgctcggttg
      361 tcgggttcga acgcacgaag ccgggcgcca ccgcgttgca ggtgatgccg tgcgggccga
      421 gatcctgact gagctgtttg gtgaggccaa ccagcgcgtg tttcgccgcg gtgtaggcct
      481 ggatgccagt caggctcggg cgcagacccg cccctgaagc gatgttgacg atacggccgt
      541 agcgcgcggc ctgcatcggc gcggccgccg cctgcgacag ccacatggcc ccgtcgacgt
      601 tggcggcgaa gatcgcgcgc cagccgtcct ctgtgacggc ctcgatcggt ccgccgacct
      661 gcccgcgcac gccgccggcc gcattgacca gcacgtcaag ccgaccgctc cgggcaagca
      721 cggcatcgac agtcgccccg caggcggacc tgtccgctat gtcggcgaca tgcgcctgca
      781 tgtgcatccc gcttgcgatg tcgcgcaccg ccgggtcgat gtcggcgaga tgcaccgtgg
      841 cgcccgaatc ccggagtgct gcggcgatgg cccggcctat gccctgcgcg ccgccggtga
      901 ccagggctac gcggttgtcg aagcgcaggt gcatgtgtcg atcaggctct ggaacgtttc
      961 gaaggccatg ggtcggcgac cgtcctcgat gtcgtggatc agcgccacca gccggcggat
     1021 tgcgggggtc tcgatgccga cttcgcgcgc gagatcggcg atgacgccga tctgcggatc
     1081 gacctcggtc ttgcgccggc gcacggcgag gtcacggtag atgccggtat gcgtcttggc
     1141 ggtgtggcgg ttgaactcgg ccagcgcggc aatggaggcg cgggcgccgg cttccggcgc
     1201 gccgggcgcg aaacatgccg gatcaaatcc gttgaacccc accgggcgga tgccccgcgc
     1261 cgccgccgtg gccatgactt cgcgggccaa acggtcgaac acgacgaagc ggcgtgggtc
     1321 ctcgaagttg gcggtcatgg aatcggtggt cagcgccgtg gcgaatagca tggcgccgta
     1381 ggccagcttg ccccagaggt agccccagat attgtccgtc agcacggcgg acggctcgaa
     1441 caggcgcagc agttcatgca tgcgccgggt ccggtcacgt atcgtgctat cgatctcgcc
     1501 gacaacgacc gcgccgcgat tgccgaacag gatttcgccg ggaccgagcc agtcggcgcc
     1561 gaaattgaca aagcagccca tggtgcgttc gggaccgaca atccgcgcga tcgccagttc
     1621 gttgagtccg ttctgggccg aaagcacgaa gccgtccggc gcgagatggg gcaggagctg
     1681 gcggcaggcg gcttcggtcg ccggcgcctt gaccgccagc acgatgacat tgaagacgcc
     1741 cttcacgtcc tcgatgcgag ccgagggaac gacctgtgtg aaatcctcaa ccgggccgct
     1801 gatgtgcagg ccttgcgtgc ggcaagcttc cacatgcgcc tcgacgatgt cggtcatcag
     1861 aaccggctgt ccggcgcgcg cccaatacgc accgagcgtg ccgccgatgg cgccggcgcc
     1921 ccagatcagg atgggcttgg cgccgctcat gcctctgccc ttccgaggtc atgcacctcc
     1981 ttgtgtgtcg ccacgaagag agcgccgccg gtggtgatcc agccgcgata catgccgatg
     2041 gtgttgaacg gcgtcacggg atctcccgtc gcacccagcg ccaccaggcc cgcgccgatg
     2101 ccgtggctcg gcagcgtctt gtgcaccacc tcgtccgtgg cctgttccag ggtctgcccc
     2161 gcataggtca tgcgcgccgc gatgtcatag gccgcgacgc gccggatgaa gatctcgccc
     2221 tgcccggtgc aggacacggc gcagacgcca tcgcgggcat aggtgcctgc tccgatgatc
     2281 ggactgtcac cgacccgacc gacgggcttg ttgttgaagc ctccggtgga ggtggcggcc
     2341 gctagatggc cggcgcgatc acgcgccacg gcgccaacgg tgccgtgctt ctcggcttcg
     2401 ctcgcggctt tcagcgtgcc ggcggcggcg cgcgccttca ggctggccag ggcgtcgatg
     2461 cggcgctgcg tcgtgaaata ggcggggtcc accgaaggag acccctcatg accggcaacg
     2521 cggctccccg cgtgcgcggc ggtcccgtgc cgatcgcgca gcattccgac aacctcaccc
     2581 atgtcgaact gacctgggtg gagaagaaga tcgaaaactg gatcaggttc ggccgcgagg
     2641 cgcgagaaac cgtcctcgac cgccgccgcc gtgtcctggt cttccggccc ggcagcatct
     2701 tcgccttcgt tcgctgggcc tccaacgact tcggcacgat catttcgcgc atcgacatcg
     2761 tgcgcgcggt caagcccggt gaagcctatc agacactgcc cttcgtgcgg cccggcggcg
     2821 acatcctcct caagatcgaa ggctggccga aggtgcagca ggtgctctgc cacatcgatg
     2881 cggtggagag cgccgggatc gacgtctgcg aggtgtccgc cgatcactgg cggcacgtcc
     2941 atcatcgcct catcgcaggc catgagccgc gcgcctacac gcaagaacgc catcaagcgt
     3001 ggctcaagcg gcgggagatc gagggatgac ccgcttcggc tatgtcatgg cgacgtattt
     3061 ctcgatcatg ggcgtcgcca tcgcgtcctt cgttccagtg ccgctgcgcc tcatctggaa
     3121 cgcctcggcc agcgtgccga tcggcctcta cgatctcgac cgcccgggtc ctctccacga
     3181 aggcgatctg gtagccgtga cgccggacga accgctcgcc gatttcatgg tcgcacgcgg
     3241 ctatatcggc cgcggcgtgc cgctgatgaa gcatgtcgcg gcccttccag gacaggaagt
     3301 ttgccggatc ggcaattccg tctccgtcga tggcgtgccg ttcggcgaag cgctcgaccg
     3361 cgaccggcgc ggccgtccgc tgcccgtctg gcggggctgc ctgcgcatcc gcgacggcca
     3421 ggtctttctc atgaatccgt ccgtgccgga cagcttggat ggccgctatt ttggcccgat
     3481 cgcggcgcga tccgtcatcg gcagggccac cccgctctac accgacgagg ccggcgacgg
     3541 tcgcttcgtc tggcgcgccg ccacgcgctg accgcatccc catcggcggg agcggtgctc
     3601 gccgatgggg cttccaacgc caccgccaag gagaccacca tgccacagat cggcacattc
     3661 atccgcgacg aaagcggctt cgtcgggcac ctgacgacct tgaacctgag ccgggatgtc
     3721 atcatcattg gcgccgaacc gtcggacgcc gagaacgccc cggactatcg cgtccacgtc
     3781 ttcgacgcga tgaacaacga gaccgccgcc gaaatcggcg cgggatggaa acgcaccggc
     3841 gaaaaggctg gcgaatacat cgccatcctc tttgacgacc cgagcttcgc acagccgatc
     3901 cgcgccaacc tgttccgcga cgacgatacc gggcggggct ggtcgctgca ctggacccgc
     3961 gccgccaagc gcgacgggaa ggactgatcc catgcgtgga accgtcctct tcctcctttc
     4021 cggcctgtcc ttcgccgctc tcctgccggt cggtgtcctc gcacagccgg cgtcgatcac
     4081 aagaccgtcg cagggaactc cctacgccga tcatatcgtc gaggcggcgc agcgcttcgc
     4141 gttgccggca gcatggatca gggcggttct gcgcgccgaa agcgccggag attcacgcgc
     4201 gatctccccc aaaggtgcga tggggctgat gcagatcatg cccggcacat gggcgaacct
     4261 gcgcatgcgt ttgtcgctag gcagcgaccc ttacgatccg cacgacaaca tcatcgcggg
     4321 cgccgcctat atccgcgagc tgttcgatcg ctacggctcg cccggctgga tcgctgccta
     4381 caatgcgggt cccggacgct atgaggcgtc gctttccggt cggccattgc ctgctgagac
     4441 acgcgcatac gtcgcctccg tctcaccggc gctcggcggc ggcagcgatt ccggcacgac
     4501 tgtcatcgct cccgccgatc ccttcgcatg gacccgcgcg ccgctgttca tcgcgcaacg
     4561 ggcaagcacg cccactgccg atcccgcgcc ggaaaaacgc gcgtcggatg atgctccggg
     4621 cgcttcatca tccccttctc tcgtgcgcga tgtttcagca atcgtgccgc agtcgaacgg
     4681 tctgttcatc gcccgcgccg acaccgggac gatgcgatga gcatggcggc tcccgatcgc
     4741 acgatagcgg gcgctggcgt ggtctggagg gcgcgcaggt ggagtgcagg caacacgacc
     4801 gtatgcgtcc ggcgcccgca cgtgatgtca gtccttgaca ggatttt
//
LOCUS       DAOMXN010000191         4844 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_9373_length_4844_cov_9.137607, whole genome shotgun sequence.
ACCESSION   DAOMXN010000191 DAOMXN010000000
VERSION     DAOMXN010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4844
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9373_length_4844_cov_9.137607"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1197)
                     /locus_tag="PK812_11575"
     CDS             complement(<1..1197)
                     /locus_tag="PK812_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015824282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRJ70236.1"
                     /translation="MHLAVRMPPVSERRKVSMDRLIELASRSHARALAALFVLSLALF
                     LPGQFSVQPMDRDEPRFAQASKQMLETGDFVDIRFQDEARHKKPVGIYWLQTASVAVA
                     ERLGVPDARGTIGYYRIPSLIGALATVLLTYWAALAFLSRPGALLAAALMAGSILLGV
                     EARLAKTDAVLTATVVAAMGALARVWFAAVLPETHYRPGLGLALVFWIAIGVGVLIKG
                     PITPLIPAIVVLILSIRERSLAWLKPLRPLMGLALVIAIALPWLAAIAWKTGGAFFAE
                     SVGKDMLGKVGGVAEKHWGPPGAYIGAFWGTFWPGAPLAALALPFAWKERRDDSLVFL
                     IAWIVPMWLILEAMPTKLPHYVLPLFPAVAILILIAVERGALAMEGWRARLAAGLMLL
                     IPLGLLV"
     gene            complement(1334..1717)
                     /locus_tag="PK812_11580"
     CDS             complement(1334..1717)
                     /locus_tag="PK812_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70237.1"
                     /translation="MKLIALVAVIGILLLQWSEAIPLSSVGGPMVIALAFFCAAMAVA
                     IHEAWTEKRGILGWIVSIVVAFLGAFVAAQLGGMVMVFLLAPFADGTSLAASGGAVMS
                     AALVGAMIVTLIGAWGALRFVKRWR"
     gene            complement(1859..1935)
                     /locus_tag="PK812_11585"
     tRNA            complement(1859..1935)
                     /locus_tag="PK812_11585"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1899..1901),aa:Arg,seq:tct)
     gene            complement(1967..2272)
                     /locus_tag="PK812_11590"
     CDS             complement(1967..2272)
                     /locus_tag="PK812_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ETC complex I subunit"
                     /protein_id="HRJ70238.1"
                     /translation="MTARIYKPARNAMQSGQGKTVRWLLEFEPAAPRQVEPLMGWTSA
                     TDMDAQVRLWFDSKEAAVAYAEKHGLAYRVEEPKETTRRSMAYADNFKFNRTGQWTH"
     gene            complement(2312..2388)
                     /locus_tag="PK812_11595"
     tRNA            complement(2312..2388)
                     /locus_tag="PK812_11595"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2352..2354),aa:Pro,seq:tgg)
     gene            complement(2440..2976)
                     /locus_tag="PK812_11600"
     CDS             complement(2440..2976)
                     /locus_tag="PK812_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019904598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HRJ70239.1"
                     /translation="MGSDTAGARPFWQRAFLVLFVVVTLGVSGYSIWSNLARQDAQAQ
                     SRSAAIAQGMEELSITTARDSHRFLVEVMRTDAERAKGLMFRQSMPHDHGMLFDFKMD
                     APVAMWMKNTYIPLDMLFITADGTIARIERRTEPHSERTIASGGPVRAVLELNGGTAE
                     RLNLKPGDRVKHAMFPAK"
     gene            complement(3049..3672)
                     /locus_tag="PK812_11605"
     CDS             complement(3049..3672)
                     /locus_tag="PK812_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HRJ70240.1"
                     /translation="MIDDGQGEKKFGDLLSGQARPSERPAKSDFAPEPELDLIQISGV
                     IKWFDVAKGYGFIIPDNGMPDILLHVTCLRRDGFQTAQEGARIVCEVVQRARGYQTFR
                     VLSMDASTAVHPAMLPAPRTNVIVTPEGNYEKAMVKWFNRLRGFGFLTRGEGTPDIFV
                     HMETLRRFGIAELRPGQWVYVRFGKGPKGEMAADIRLADSVSGPLSH"
     gene            complement(3810..4580)
                     /locus_tag="PK812_11610"
     CDS             complement(3810..4580)
                     /locus_tag="PK812_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HRJ70241.1"
                     /translation="MSADPAAVEDLADRLRGARRCVGFTGAGISTESGIPDFRSPGSP
                     WLSNKPVPFQAFVASEEARREAWRRKFRMDDHYAGAAPSMGHRFLADLVVTGRMPAII
                     TQNIDGLHQAAGVPPEQVIELHGNGTYATCLDCGRRHELVDVRARFETTGAAPRCDAC
                     DGLVKSATISFGQSMPEAQMRRARHEAQRCDLFLAMGSSLVVFPAAAFLEVAKRAGAT
                     LVIVNREATEFDPLADLVVRGEIGSVVAGVIDRLGSGR"
     gene            complement(4577..>4844)
                     /locus_tag="PK812_11615"
     CDS             complement(4577..>4844)
                     /locus_tag="PK812_11615"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387652.1"
                     /note="catalyzes the formation of pyruvate and glyoxylate
                     from 4-hydroxy-2-oxoglutarate; or pyruvate and
                     D-glyceraldehyde 3-phosphate from
                     2-dehydro-3-deoxy-D-glyconate 6-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="keto-deoxy-phosphogluconate aldolase"
                     /protein_id="HRJ70242.1"
                     /translation="LTLREAGFRALKFFPAVAAGGLEYLKSVAGPCPDLKFCPTGGIG
                     PGNMRAFLAEPNICAVGGSWLTPKAAMQNGDFAAITRLAAEATG"
BASE COUNT          853 a         1637 c         1624 g          730 t
ORIGIN      
        1 caccagcagc cccagcggga tcagcagcat gaggccggcg gcaaggcgcg cgcgccagcc
       61 ttccatggcc agggcgccgc gctcgaccgc gatcaggatc aggattgcca cggccgggaa
      121 cagcggcagc acatagtgcg gcagcttcgt cggcatggcc tcgaggatca gccacatcgg
      181 cacgatccag gcgatcagga aaacgagcga atcgtcgcgc cgttccttcc aggcgaaggg
      241 tagcgccagc gccgccagcg gcgcgccggg ccagaacgtg ccccagaacg ccccgatata
      301 ggcgccgggc ggtccccaat gcttttccgc gacgccgccg accttgccga gcatgtcctt
      361 gccgacggat tcggcgaaga acgcgccgcc ggtcttccag gcgatggcgg cgagccaggg
      421 cagcgcgatg gcgatgacga gggcgaggcc catcagcggg cgcagcggct tcagccaggc
      481 gagcgaacgc tcgcggatcg acaggatcag cacgacgatg gccgggatca gcggcgtgat
      541 cggccccttg atcagcacgc cgacgccaat ggcgatccag aacaccagcg caaggccgag
      601 accgggcctg tagtgcgtct cgggcaggac ggcggcgaac cagacgcgcg ccagggcgcc
      661 catcgcggcg accaccgtcg cggtcagcac cgcatcggtc ttggccagcc gcgcctcgac
      721 gccgagcagg atggagccgg ccatcagcgc cgcggcgagc agcgcgccgg gacgcgagag
      781 gaaggcaagc gccgcccaat aggtcagcag caccgtggca agcgcgccga tcagcgaggg
      841 aatgcggtaa tagccgatgg ttccgcgcgc gtccggcacg ccgagccgtt cggcaacggc
      901 cacgctggcg gtctgaagcc agtagatgcc gaccggcttc ttgtggcgcg cctcatcctg
      961 gaagcggatg tcgacgaaat cgccggtctc cagcatctgc ttggaggcct gcgcgaagcg
     1021 cggctcgtcg cggtccatcg gctgcaccga aaactgcccg ggcaggaaca gggccagcga
     1081 cagcacgaac agcgcggcga gagcccgtgc gtgcgaccgt gaggcaagct cgatcaggcg
     1141 gtccatgctc accttacgcc tttcagaaac tgggggcatg cggacggcga ggtgcatcgc
     1201 cggccggcgt cctgccggca cctgcgcgct ggacgggcaa gaccactggg ttgatttcca
     1261 tggacagcgc ttagcgaagc gcgccaccca ccgtcaactg cgcgccttgc ctcgtgcgat
     1321 gcagtcgagg gctctaccgc cagcgtttga cgaaccgcaa cgcgccccac gcgccgatca
     1381 gtgtcacgat catcgcaccg acaagcgccg ctgacatgac cgcgccgccg ctggccgcca
     1441 gagaggtgcc atcagcgaag ggcgcaagca ggaacaccat gaccatcccg cccagttgcg
     1501 ccgcgacgaa cgcgccgaga aaggcgacca cgatactgac gatccagccc agaatgcccc
     1561 gcttttccgt ccatgcctcg tggatcgcca cggccatcgc ggcacagaaa aaggccagag
     1621 cgatcaccat gggcccaccg acgctcgaaa gcggaatggc ctcggaccac tggagaagca
     1681 ggatgccgat cacggcaacc agggcaatca gtttcatggc taagtcccgc caggacatca
     1741 ggattggcac gctcacggac gctagcgggg aacgggcggg gccgcaaatc acgggctgtt
     1801 gcatgccaca gtgccggggc atcgcgcgcc tcgccggtcc cggctcaaag atcctgaatg
     1861 gcgaccccgg cagggttcga acctgcgacc tcatgcttag aaggcacgtg ctctatccag
     1921 ctgagctacg gggccgaaaa ccggcggcgg cctgggttcg cgccggtcag tgcgtccact
     1981 ggccggtgcg gttgaacttg aaattgtccg cgtaggccat cgaccggcgg gtcgtctcct
     2041 tcggctcctc gacgcggtag gcgaggccgt gcttctcggc ataggcgaca gcggcctcct
     2101 tgctgtcgaa ccacaggcgc acctgcgcgt ccatgtcggt cgcggacgtc cagcccatca
     2161 gcggttcgac ctggcgcggc gcggccggct cgaattcgag cagccagcgg acggtctttc
     2221 cctgcccgga ctgcatggcg ttgcgggctg gcttgtagat gcgggcagtc atgcctctca
     2281 cctcatggtc gtgttcgaac cccggtccgg ctggtcggag tggcaggatt tgaacccgcg
     2341 accctctgct cccaaagcag atgcgctacc agactgcgct acactccgag gcgccgaacc
     2401 ttcgccgggc tatcacgtcg cgggccggcg gggcaaggct cactttgccg gaaacatcgc
     2461 gtgcttcacg cgatcgcccg gtttcaggtt gaggcgctcg gccgtgcccc cgttcagttc
     2521 cagcaccgcg cgcaccggac cgccggaggc gatcgtgcgc tccgaatgcg gctcggtgcg
     2581 ccgttcgatg cgggcgatcg tgccgtccgc cgtgatgaac agcatgtcga gcgggatgta
     2641 ggtattcttc atccacatcg ccaccggcgc atccatcttg aaatcgaaca gcatgccatg
     2701 gtcgtgcggc atggactggc ggaacatcag gcccttggcg cgttccgcgt cggtgcgcat
     2761 cacctcgaca aggaagcgat ggctgtcgcg cgccgtcgtg atgctgagtt cttccatgcc
     2821 ctgggcgatg gcagcggacc gcgactgggc ctgggcatcc tgacgcgcga ggttcgacca
     2881 gatcgaatag ccgctcacgc cgagcgtcac gacgacgaat aagacaagaa aggcccgctg
     2941 ccagaacggc cgcgcgcctg cggtgtcaga acccatgatg ctcgtccgca gttggcacgg
     3001 acgcttccat acgcccgcgc ggcccccggc tcaagggcgc ggcgacgctc agtgcgagag
     3061 cgggccggat acgctgtccg ccagccggat gtcagcggcc atctcgccct tcggaccctt
     3121 gccgaagcgg acgtagaccc actggcccgg acgcaactct gcgatgccga agcggcgcag
     3181 cgtctccatg tgcacgaaaa tgtcgggcgt tccctcgccc cgcgtcagga agccgaagcc
     3241 gcgcaggcgg ttgaaccact tcaccatcgc cttctcgtag ttgccttccg gcgtgacgat
     3301 gacgttggtg cgcggggccg gcagcatcgc gggatgcacg gccgtcgagg cgtccatgga
     3361 gagaacgcgg aaggtctggt agccgcgggc gcgctgcacc acctcgcaga cgatgcgggc
     3421 gccttcctgg gcggtctgga acccgtcgcg acgcaggcag gtgacgtgca gcaggatatc
     3481 cggcatgccg ttgtcgggaa tgatgaagcc gtatcccttg gcgacatcaa accacttgat
     3541 gacaccggaa atctggatca gatccagttc cggttccgga gcgaagtcgc tcttggctgg
     3601 tctttcggat ggcctcgcct gcccggaaag cagatcgccg aacttctttt cgccctgtcc
     3661 gtcatcgatc attgattcga cccgcctact ccgattcccc cgcgaatctc tcgacacaat
     3721 agcattcgtg cagggtctgc aaactgttcg tgcaggttct cacagaaaaa tatggaactc
     3781 ttgatcggct ggattggccc ggctccgctt caccggccgg agccgagcct gtcgatgacg
     3841 ccggcgacca cggaaccgat ttcgccgcgc accaccaggt cggccagcgg atcgaattcg
     3901 gtggcctcgc ggttgacgat gaccagcgtc gcgccggcgc gcttggccac ttccaggaag
     3961 gcggcggcgg gaaagaccac gagcgaggac cccatggcca ggaacaggtc gcagcgctgc
     4021 gcctcgtgcc gggcgcggcg catttgcgct tccggcatcg actggccgaa ggagatggtc
     4081 gccgatttga cgaggccgtc gcacgcgtcg cagcgcggcg ccgcgcccgt ggtctcgaag
     4141 cgcgccctga catcgacgag ttcatggcgc cggccgcaat cgaggcaggt ggcgtaggtg
     4201 ccgttgccgt gcagttcgat cacctgctcg ggcggcacgc cagcggcctg atgcagcccg
     4261 tcgatgttct gcgtgatgat ggccggcatg cggcccgtca ccaccagatc ggcaaggaag
     4321 cgatgcccca tcgagggcgc ggcgccggca tagtggtcgt ccatgcggaa cttgcggcgc
     4381 caggcctcgc ggcgcgcctc ctcgctggcg acgaaggcct ggaacggcac gggcttgttg
     4441 ctcagccagg ggctgccggg cgagcggaag tcgggaatgc cgctttcggt cgagatgcct
     4501 gcgccggtga agccgacgca gcgccgcgcg ccgcgcagcc ggtccgccag gtcctcgact
     4561 gccgccggat cggcgctcat cccgttgcct ccgcggcgag gcgtgtgatc gcggcgaaat
     4621 ccccgttctg catcgcggcc ttcggggtca gccaggagcc gccgaccgcg cagatgttcg
     4681 gctcagccag aaacgcgcgc atgttgcccg ggccgatgcc gccggtcggg cagaacttca
     4741 ggtctggaca ggggccggcc acggatttca gatactcaag cccgccggcg gccacggccg
     4801 ggaagaactt caacgcgcga aatccggcct cgcgcagcgt cagc
//
LOCUS       DAOMXN010000192         4811 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_9444_length_4811_cov_11.736333, whole genome shotgun sequence.
ACCESSION   DAOMXN010000192 DAOMXN010000000
VERSION     DAOMXN010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4811
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9444_length_4811_cov_11.736333"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="PK812_11620"
     CDS             <1..790
                     /locus_tag="PK812_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006332255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-methylproline demethylase"
                     /protein_id="HRJ70243.1"
                     /translation="GKAGGQLLLATRNPRRRELIGIVDWRLSELARLGVVPRYNHYVH
                     PADVDALQPEAVIVATGGLPQAPALEAGQELAISSWDILSGSAKIAETVLLYDDNGAH
                     PGMSAAELIARAGARLEIVTPERMLAPDMGPFNHVPYMQAFRRQGAQLTIATRLKSVR
                     RDGNKLVATLSSDYAPDVIEERRVDQVVVEHGTLPAEDLYTALLPRSRNGGEIDYAAL
                     VAHRMPFPMRRPEGRFVLLRIGDAVQSRNVHAAIYDGLRYGLLL"
     gene            841..1932
                     /locus_tag="PK812_11625"
     CDS             841..1932
                     /locus_tag="PK812_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016556080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRJ70244.1"
                     /translation="MATKRQDRITIADVARRAGVSTATAGRVLGGYGYASEEIRSRVR
                     KSAEALGYRPNLLARGLITGRTKTIGVVAGDVQSPFYASVLRGISDVARAEGFGVIVT
                     NSDERTDREVEAVQLLLEKQVDGLIVAPSDLLRPAHLHAAVEGGWPIVQIDRVVAGLA
                     ADSVTVDNRGAAREAVAHLIAVGHRRIGIVAELERSEFPGVEAFVRAVAGGLVPTASL
                     FPSWQRLYGYLDAHRDAGLSVDPGLIARVGEYSVGAGREETLKLLRHPHRPSALFTAD
                     GLMSAAAMDAITTQGIAIPGDLSLVCFDDLDWMRFSKPGVTAIVQPLIALGEAAATIV
                     LARIKGECGADRHVVLATSLAPRGSVATP"
     gene            complement(2017..3129)
                     /locus_tag="PK812_11630"
     CDS             complement(2017..3129)
                     /locus_tag="PK812_11630"
                     /inference="COORDINATES: protein motif:HMM:NF040876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHE_PE00001 family protein"
                     /protein_id="HRJ70245.1"
                     /translation="MNSINQISALEKLLDGVAKLPWATIAGPLVAAEDALARLDERLR
                     SSPVREGWIARSHVRDVVACIWVSGELVTIEDLVLRDAGMDVRTPSAVLSKAQEILRV
                     RRRLAGGAGASVLSREGLDALLGQTSTEYPPVETGNPSSSALFAGDDGLRDALSRADV
                     AVAEVRAVLGRIEQSAQISRNPLVYDLDWNEGERLQSWFGTIGEFKSFPPVLSSVLAL
                     CAWDAIEPLQHQSWLGRFLAAAMLRERDKARAHLPCLYSGLRVSGRGPRRPETFLAAI
                     QGEIKAVEAAARDGLKEHDRLMLARLQFDQRLKGRRSTSRLPELVEFVLETPLISTAL
                     VARKLRVSQRAAQDLILGLGLRELTGRERYRAWGIV"
     gene            complement(3162..4481)
                     /gene="repC"
                     /locus_tag="PK812_11635"
     CDS             complement(3162..4481)
                     /gene="repC"
                     /locus_tag="PK812_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018409469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication protein RepC"
                     /protein_id="HRJ70246.1"
                     /translation="MAHGLATTPFGRRTMSLGLMAAQVNARNATREAPDKTVHKWKVV
                     QALTEAKDRLGIPDRAIAVLNALLSFHQETTMTAGDGLVVFPSNAKLAARAHGVAPTT
                     LRRHLAILVEAGIVIRRDSPNGKRYARRGEGGEISEAFGFDLAPLVARAEEFDRLAAE
                     VQAERKALRLARERVTLARRDITKLIALGLEEGIPGDWASLHAAYLGLVGQLKRSATT
                     ETLLACAADLEDMASEIAKHLELFVNSQNAGGNESQNGHHKQNSNTDPYDLEPAFKKD
                     RAETAELDRQGLGSVEPGPEIEAGTPREPGRDTGTPLGMVLQACPDIVEYAGTGAGEA
                     PNGKPAIANWAAFLKTAALVRAMLGISPDAWRDATETMGVVDASIVVAAILQKGDEIR
                     FPGGYLRSLTEKKRVGEFSVGPILMALINGQLKARKAKGQGANRMPE"
BASE COUNT          813 a         1579 c         1515 g          904 t
ORIGIN      
        1 cggcaaggcg ggggggcagt tgctgctggc gacgcgcaac ccgcgccggc gcgagctgat
       61 cggcatcgtg gactggcggc tctcggaact ggcgcggctc ggcgtggtcc cgcgctataa
      121 ccactatgtg catcccgccg acgtggacgc tttgcagccg gaggctgtca ttgtcgcaac
      181 cggcggcctg ccgcaggcgc cggcgctgga ggccgggcag gagctcgcga tctccagctg
      241 ggacattctg tccggctcgg ccaagatcgc ggagaccgtg ctcctctacg acgacaacgg
      301 cgcccacccg ggcatgtcgg cggcggagct gatcgcccgc gccggcgcgc ggctcgagat
      361 cgtcacgccg gaacggatgt tggcgcccga catggggccg ttcaatcacg tgccttacat
      421 gcaggccttc cgccgccaag gcgcgcagct gactatcgca acccgcttga agtcggtgcg
      481 ccgggacggc aacaagctgg tcgcgacgct ctcctcggac tatgcaccgg atgtgatcga
      541 ggagcggcgc gtcgaccagg tggtggtcga gcacggcacg cttccggcgg aggatctcta
      601 tacggcgctt cttccacgct ccagaaacgg cggcgagatc gactacgccg ccctggtcgc
      661 ccaccgcatg ccgtttccga tgcggcgacc ggaagggcgc ttcgtcctgc tcaggattgg
      721 ggacgccgtt cagtctcgaa acgtccatgc cgcgatctat gacgggttgc gctatggatt
      781 gctgttataa ggccgctctc cagcattgac ctgcgccttc cttgcgactg cattgggaga
      841 atggccacga aacgccagga ccgcatcacc atcgccgacg ttgcgcgccg cgccggcgtc
      901 agcacggcta cggctggccg tgtgctcggc ggctatggct acgccagcga agagattcgc
      961 agccgggtgc gcaagagcgc cgaggctctg ggctatcgcc ccaatctcct cgcgcgtggc
     1021 ctcatcaccg gacgcaccaa gaccatcggt gtcgtcgccg gcgacgtgca gagtccgttc
     1081 tacgcaagcg tgctgcgcgg catatccgac gtggcgcgcg ccgaagggtt cggcgtgatc
     1141 gtcaccaaca gcgacgagcg caccgaccgc gaagtcgagg cggtgcagct cctcctcgag
     1201 aagcaggtgg atggcctgat cgtcgccccg tcggacctgc tgcgaccggc tcatctgcac
     1261 gctgccgtcg agggcggctg gcccatcgta cagatcgatc gcgtcgtcgc tggacttgcc
     1321 gccgattcgg tgaccgtcga caatcgcggc gcggcgcgcg aggccgtcgc ccacctcatt
     1381 gcagtggggc atcgccggat tggcatcgtg gctgagctgg agcgttccga atttcctggg
     1441 gttgaagcct tcgtgcgggc cgtggctggc ggcctagtgc cgaccgcatc attgtttccg
     1501 agctggcagc ggctctatgg ttatcttgac gcccatcgcg acgcagggct ttccgtcgac
     1561 cccggtctga ttgcgcgggt gggtgaatac tccgtcggcg ccggcaggga ggagacgctg
     1621 aagctccttc gacaccccca tcgcccgagc gccttgttca cggcggacgg gctgatgtcg
     1681 gccgcggcga tggacgcgat cacgacgcag ggcatcgcca ttcccggcga tctgtctctg
     1741 gtatgtttcg acgatctgga ctggatgcgg ttttcgaagc ctggcgtcac ggccatcgtc
     1801 cagccgctta tagcgttggg tgaggctgcg gcgaccatcg tcctcgctcg catcaagggg
     1861 gagtgtgggg cagatcgcca tgtcgtcctg gccacgtcgc ttgcgccgcg cggctccgtg
     1921 gcgacgccat gaaccgcact cgcggcttcc atgtcgcggg gctttaggcc tgaaattgga
     1981 tttgaacagc gtatctcatt ctttccggcg acggagctaa acaatgcccc aggccctgta
     2041 gcgctctcgt cccgtgagtt cgcgcaggcc gagaccgagg attaggtcct gagcggcgcg
     2101 ttgcgagacg cgcagcttcc gggcaaccag ggcggtggag atcagcggcg tttcaaggac
     2161 aaattcgacg agttcgggaa gtcgcgatgt cgagcggcgg cccttaagcc gctggtcgaa
     2221 ctgaagccgg gcaagcatca gccgatcatg ctccttgagc ccgtcccggg cggctgcctc
     2281 tacggccttg atctcgccct gaatggcggc aaggaacgtt tcgggacgcc gggggcccct
     2341 gcccgagacg cgcagccctg aatacaggca cggtagatgc gcccgagcct tgtcgcgctc
     2401 gcgcaacatc gccgcggcga gaaagcgccc aagccaggac tggtgctgaa gcggttcgat
     2461 cgcgtcccag gcacacaggg ccaggacgct cgacaacacc ggcggaaagc tcttgaactc
     2521 tccgatcgtc ccgaaccagc tctggaggcg ctccccctcg ttccaatcca gatcgtagac
     2581 caggggattg cgcgagattt gcgcggactg ctctattctg ccaagcacgg ctcgcacctc
     2641 agcaacggcg acatcagccc tgctcagcgc gtcgcgaagc ccgtcatcac ccgcaaacaa
     2701 tgctgatgag gagggattcc cggtctcgac cgggggatac tcagtgctcg tttgtccgag
     2761 cagggcgtcc agcccttctc gcgacaagac cgaggcccct gccccaccag ccaggcggcg
     2821 gcgaacgcgt aaaatttcct gagctttgga gaggaccgcc gacggtgtcc ggacatccat
     2881 gccggcatcg cgcagaacca ggtcctcaat ggtcaccagc tcacccgaga cccagatgca
     2941 ggccacgacg tcccggacat gcgaccgggc gatccatccc tcccgcaccg gactggatcg
     3001 aagccgctcg tcgagccgtg ccagcgcatc ctccgccgca accaacggac ctgcgatcgt
     3061 ggcccaggga agctttgcca caccgtcgag gagcttttcc agggcgctga tttggttaat
     3121 cgaattcatc ggaacggaag ctatcacaag cttcgcctct gtcactcagg cattctgttt
     3181 gccccctgcc ctttcgcctt cctcgctttc agttggccat tgatgagcgc catcaggatc
     3241 ggtccgacgg agaactctcc gacccgcttc ttctcggtga gggagcgcag atagcccccg
     3301 gggaaacgga tttcatcgcc cttctgcagg atcgcggcga cgacgatgga ggcgtcgact
     3361 acgcccatgg tctcggtcgc gtctcgccag gcgtcaggcg aaatcccgag catggctcgg
     3421 acgagggcgg cggttttcag gaacgccgcc caattggcga tggccggctt tccattcggc
     3481 gcctcccctg caccagtgcc ggcgtattcg acgatgtccg ggcaggcctg caacaccatg
     3541 ccgaggggcg tgccagtgtc cctgcccggt tctcgaggcg tgccggcttc gatttccggc
     3601 ccgggttcga cacttcccag cccttgccgg tctagctcgg cggtttcggc cctgtctttc
     3661 ttgaaagcag gttcaagatc ataggggtcg gtatttgaat tctgtttgtg atgtccgttt
     3721 tgggactcat tgccgccggc attttgagaa ttgacgaaaa gttccagatg cttggcgatt
     3781 tccgaggcca tgtcctcgag atcagcagcg catgcgagca gagtttcggt cgttgccgag
     3841 cgcttcagct gtcccacgag accgagataa gcggcatgga ggctcgccca gtcgcccgga
     3901 atgccctcct ccagccccag cgcgatcagc ttggtgatgt cgcgccgcgc cagcgtcacg
     3961 cgctcgcgtg ccaaccgcag cgcttttcgc tcggcctgga cctccgccgc cagacgatcg
     4021 aactcctccg cccgcgccac cagcggcgca aggtcaaacc cgaacgcttc cgatatctcg
     4081 cccccctccc ctcgccgcgc gtagcgcttg ccgttcgggc tatcccggcg gatcacgatc
     4141 cctgcctcca ccaggatcgc gagatgccgc cgcagcgtcg tgggcgccac gccatgcgcg
     4201 cgggccgcga gtttggcgtt cgaaggaaac acgacaagcc cgtcacccgc cgtcattgtc
     4261 gtctcctgat ggaacgacag aagcgcgttc aggaccgcga tcgcccgatc cggaatcccg
     4321 agcctgtcct tggcctcggt cagagcctgg accaccttcc acttgtggac ggttttgtcc
     4381 ggagcctcgc gcgtggcgtt tcgcgcattc acctgtgcgg ccatcaggcc aagtgacatc
     4441 gttcgccgcc caaagggcgt cgttgccaat ccgtgtgcca tcgtctttgc ccttcaatca
     4501 ggcaaaagaa atccggccgc caaaacgacg gttgtcccaa cgggactctt gacagcgatt
     4561 cgcggaagtg ggattctcaa ggtgctgaaa tcaagagaag gcttccggcg gaaacgttgg
     4621 gggccttttt cttttgcgct ttgctcctgt tttcggtcag aactgtgaag ccgatctggc
     4681 ttcacttaat ctcgtttttg cgcttggaac tcacggaaca aatcggccat tcgggttaaa
     4741 aggaacgaac cgaatcgagg tgcgatgcgc tcgtcgacaa ccaatgtggt gtgggtggga
     4801 tcgcgcacta t
//
LOCUS       DAOMXN010000193         4804 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_9458_length_4804_cov_9.819120, whole genome shotgun sequence.
ACCESSION   DAOMXN010000193 DAOMXN010000000
VERSION     DAOMXN010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9458_length_4804_cov_9.819120"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="PK812_11640"
     CDS             complement(<1..651)
                     /locus_tag="PK812_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012317509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HRJ70247.1"
                     /translation="MVTALERWLGPTIAEAQARGADSFGVLRLLLALAVVFSHMGSVT
                     TGDIAAEPLVRSTGFSLGEHAVNGFFAVSGFLVAMSYERRGLKAYALARALRIAPGLV
                     AAALVCALGIGALMTTKALGAYLTDGGTWRFVGQTIFTFKSNAELPGVFQSNPFRFVI
                     GTVWTLKYEIYCYIGLGLAGALGLLRRRDLVLAAGLALFVAIMALDVARPDAGKAVQ"
     gene            complement(651..1538)
                     /locus_tag="PK812_11645"
     CDS             complement(651..1538)
                     /locus_tag="PK812_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017484156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRJ70248.1"
                     /translation="MASPLSAIIVTHDSAGVLPSCLAALAGQGVPALVVDNASEDETV
                     AIAERAGARVVRNAKNEGFGRANTIGVKAAETEFCLLVNPDLVLQPGAVAMLLAAAER
                     YPDAGMLAPRIVEPDGRVFYQNRSLLSESVLRRPVEGDARAVVPEGDACAPFLSGACL
                     MIRRNLFLELGGFDPEIFLFYEDDDLCRRVLEKGLSLVHVDNAVAHHARGQSSTPRPG
                     RRYRARWHMGWSRVYIARKYGLKSDAMRTILLNAPKALLSALTVQRTEYERYAGIVAG
                     TVAALRGHSALAREGLERP"
     gene            complement(1539..3314)
                     /gene="aspS"
                     /locus_tag="PK812_11650"
     CDS             complement(1539..3314)
                     /gene="aspS"
                     /locus_tag="PK812_11650"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HRJ70249.1"
                     /translation="MHRYRSHTCGGLSAADIGQIARLSGWCHRVRDHGGVLFIDLRDH
                     YGMTQIVVDPDSPAFNAAEKVRSEWVIRIDGKVRRRPAGTENANLATGEVEVFVTELE
                     VLSTASDLPLPVFGDVDYPEDTRLRYRFLDLRREGLHKNIMTRVAVIDSLRRRMKTAG
                     FNEFSTPILTASSPEGARDFLVPSRIHPGKFYALPQAPQQYKQLLMMAGFDRYFQIAP
                     CFRDEDPRADRLPGEFYQLDIEMSFVEQEDVFAAMEPVIRGCFEEFAGGKRVTPSPWP
                     RIPYAESMQKYGSDKPDLRNPIVMQDVSEHFKGSNFKVFARMLEDPKNRVWAIPAKTG
                     GSRAFCDRMNSWAQGEGQPGLGYVMWREGGEGAGPIANNIGPERTAAIRDQLGMGEGD
                     AAFFVAGNPEKFVKFAGSARNKVGEELKLTDQNSYEFAWIVDFPMFEYNEDDKKVDFS
                     HNPFSMPQGGLDALKTQDPLTIKAFQYDIACNGFELASGGIRNHKPEAMVKAFEIAGY
                     SEQTVIEQFGGMYRAFHYGAPPHGGMAAGVDRIIMLLCGAENLREVALFPMNQQALDL
                     LMGAPSDASNKQLRELHIRLNLPEK"
     gene            complement(3398..>4804)
                     /locus_tag="PK812_11655"
     CDS             complement(3398..>4804)
                     /locus_tag="PK812_11655"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRJ70250.1"
                     /translation="GTRPHRHRHLRSGGGAMRRVALAAVVTVLASLPVKAGPDILRER
                     TVAGIAYKRIPLGEAPMQTIQFLWLDGSPLALAGKAALSALGPAMMHEGPRGEKRGEI
                     QELMSDLQASFRFAASGETVTLTLTAPPESLPAAANVLKRFLEAPGFPERRLETHRRA
                     RILGTRQSLASGDTIAGQLRLRFLLEPGAHRDFLRIDPEHYATVSVADVADWKARVMA
                     RDTAIIATSGPVGREAIAAEIDKLVGVLPERARRSDPPPLAFRSFGKRIVYEKPDAAQ
                     TIIVAYGPTTIAPGRERFKADLALARLGGGAQGRLFRVLREKLGATYGVSAGTDQMAL
                     GRFAFAIRTSVSHDKAAAVIEEIGREYRRWREDGVTDAELQAAKSQARSSWSESMKQP
                     GTVASMAVLAEVIGLGDVTFADFAAAREAVTREEINAYIRAHFPQWPLGLVIVAPKAD
                     GLGADCVIKALEEAARCE"
BASE COUNT          816 a         1653 c         1569 g          766 t
ORIGIN      
        1 ctgcaccgcc tttccggcgt cgggccgggc gacatccagc gccatgatgg cgacgaacag
       61 cgccaagccc gccgccagca cgaggtcccg gcgccgcagc aaccccagcg cacccgccag
      121 ccccagaccg atgtagcaat agatctcgta tttcagcgtc cagaccgtgc cgatgacgaa
      181 gcggaacgga ttggactgga acacgccggg caattcggca ttgctcttga aggtgaagat
      241 ggtctgcccg acgaaccgcc acgtgccgcc atccgtcagg taggcaccga gcgccttggt
      301 cgtcatcaat gcgccgatcc ccagcgcgca gaccagcgcc gccgcgacca gtccgggggc
      361 gatgcgcaac gcccgcgcca gcgcataggc cttcagcccg cgtcgctcgt agctcatggc
      421 gacgagaaac ccggacacgg cgaaaaaccc gttcaccgcg tgctcgccca gcgaaaagcc
      481 agtggaccgg accagcggtt cggccgcgat gtcgcccgtg gtgacgctgc ccatgtggga
      541 aaacaccacg gccagcgcca ggaggagacg cagcacgccg aaactgtcgg ctccgcgtgc
      601 ctgcgcttcc gcgatcgtcg ggccgagcca acgctccagg gcggtcacca tcagggccgc
      661 tccagccctt cgcgcgccag cgcgctatgg ccccgcagcg ccgcgaccgt tcccgcgacg
      721 atgccggcat agcgctcgta ctccgtccgc tggaccgtca gcgccgacag gagcgccttg
      781 ggggcattga ggaggatcgt gcgcatggcg tcggacttca gcccgtattt ccgggcgatg
      841 taaacccggg accagcccat gtgccagcgc gcccggtaac gccgccccgg cctcggtgtc
      901 gaggattgcc cacgcgcatg gtgggcgacg gcgttgtcca catgcacgag tgacaggccc
      961 ttctccagca cgcggcggca gagatcgtca tcctcgtaga acaggaaaat ctcgggatcg
     1021 aacccgccaa gctccaaaaa caggtttcgg cggatcatga ggcatgcgcc ggacaggaac
     1081 ggcgcgcagg catcgccctc cggcaccacg gcgcgggcgt ccccctccac cgggcggcgc
     1141 agcacggatt cgctcagcag cgagcggttc tggtagaaga cgcgtccgtc cggctcgaca
     1201 atgcgagggg ccagcatgcc ggcgtccggg taacgctccg ccgccgccag caacatcgcc
     1261 acggcgccgg gctgaagcac aaggtccggg ttcaccagca gacagaattc ggtttcggcg
     1321 gccttcacgc cgatggtgtt ggcgcgccca aaaccctcgt tcttcgcatt gcggacgacg
     1381 cgcgccccgg cacgttcggc aatcgcgacc gtctcgtcct cgctggcatt gtcgaccacc
     1441 agcgccggca caccctgccc cgccagcgcg gcgaggcaag aaggcagcac acccgcgctg
     1501 tcatgggtga cgatgatggc ggagagcggc gaagccactc acttctccgg caggttcagc
     1561 cggatgtgca attcgcgcag ctgcttgttc gaggcgtcgc tcggcgcgcc catcagcagg
     1621 tcgagcgcct gctggttcat cgggaacagc gccacttcgc gcaggttttc cgcgccgcag
     1681 agcagcatga tgatgcggtc gacacccgcc gccatgccgc cgtggggcgg cgcaccgtag
     1741 tggaaggcgc gatacatgcc gccgaactgc tcgatcaccg tctgctcgga gtagcccgcg
     1801 atctcgaagg ccttcaccat ggcttccggc ttgtggttgc ggatgccgcc ggacgcgagt
     1861 tcaaagccgt tgcaggcgat gtcgtactgg aacgccttga tggtcagcgg gtcctgcgtc
     1921 ttcagcgcgt ccagcccgcc ctgcggcatc gagaacgggt tgtgcgagaa atcgaccttc
     1981 ttgtcgtcct cgttgtactc gaacatcggg aagtcgacga tccaggcgaa ttcataggaa
     2041 ttctggtcgg tgagcttcag ttcctcaccc accttgttgc gggccgagcc agcgaatttc
     2101 acgaactttt ccgggttgcc ggcgacgaag aaggcggcat cgccctcacc catgccaagc
     2161 tgatcgcgga tcgccgcggt gcgctccggc ccgatgttgt tggcgatcgg ccctgcccct
     2221 tcgccgccct cgcgccacat aacgtagccg aggcccggct ggccctcgcc ttgcgcccag
     2281 gagttcatgc ggtcgcagaa ggcgcgcgag ccgccggtct tcgccggaat ggcccagacc
     2341 cggtttttcg ggtcttccag catgcgcgcg aagaccttga agttcgagcc cttaaaatgc
     2401 tcggacacgt cctgcatgac gatggggttg cgcaggtccg gcttgtcgga accgtatttc
     2461 tgcatcgact cggcatacgg gatgcgcggc caggggctcg gcgtcacgcg cttgccgccc
     2521 gcgaattcct cgaaacagcc acggatcacc ggctccatcg ccgcgaacac gtcctcctgc
     2581 tcgacgaagc tcatctcgat gtcgagctga tagaattcgc ctggcaggcg atcggcgcgc
     2641 gggtcctcgt cgcggaaaca cggcgcaatc tggaaatagc ggtcgaaccc cgccatcatc
     2701 agaagctgct tgtactgctg cggggcctgc ggcagcgcat agaacttgcc cggatggatg
     2761 cggctcggca caaggaagtc acgcgcgcct tccggggacg aggccgtcag gatcggcgtc
     2821 gagaattcgt tgaagcccgc tgtcttcatg cgtcggcgca gcgaatcgat caccgcgacg
     2881 cgggtcatga tgttcttgtg caggccctcg cgcctgagat cgaggaagcg atagcgcagc
     2941 cgcgtgtcct cgggatagtc gacatcgccg aacaccggca gcggcaggtc cgaggcggtg
     3001 gagagcactt caagctcggt gacgaagact tccacctcgc cggtcgcgag attggcgttt
     3061 tccgtgccgg cagggcggcg gcgcaccttg ccgtcgatgc ggatcaccca ttccgaacgc
     3121 accttttccg ccgcgttgaa ggcgggggaa tccgggtcca ccacgatctg cgtcatgccg
     3181 taatgatcgc gcaggtcgat gaacagcacg ccgccgtggt cgcggacgcg gtggcaccag
     3241 ccggaaagcc gggcgatctg gccaatgtcg gcggcggaaa gcccgccgca ggtgtgggaa
     3301 cgatagcgat gcatggaaaa tccgtcgaaa acgtgcggtg gcggtcatgc gcgccggtgc
     3361 gccgggtgtc aagggaaagg gcgcatggcg aggttcgtca ctcgcagcgc gccgcttcct
     3421 ccagcgcctt gatgacgcaa tcggcgccga ggccgtcggc tttcggcgcg acgatgacga
     3481 ggcccagcgg ccactgcggg aaatgcgcgc ggatataggc gttgatctcc tcgcgcgtca
     3541 cggcttcgcg cgcggcggca aaatctgcga aggtcacgtc accaaggccg atgacctcgg
     3601 cgagcaccgc catcgacgcc accgttcccg gctgcttcat gctttcggac cagctgctgc
     3661 gcgcctgcga cttcgctgcc tgaagctccg cgtcggtcac gccgtcctcg cgccagcgcc
     3721 ggtattcgcg gccgatctcc tcgatcactg ccgccgcctt gtcatgcgag accgaggtgc
     3781 ggatcgcgaa ggcgaagcga ccgagcgcca tctggtcggt gccggccgat accccatagg
     3841 tcgcgccaag tttctcgcgc agcacgcgga aaagccgccc ctgcgcgccg ccgcccagcc
     3901 gcgccagcgc cagatcggcc ttgaaccgct cccggcctgg ggcgatcgtg gtgggaccgt
     3961 aggcaacgat gatggtctgc gccgcatccg gcttttcata gacgatgcgc ttgccgaagc
     4021 tccggaaggc cagcggcggc ggatcggacc tgcgggcgcg ctccggcagg acgccaacca
     4081 gcttgtcgat ttcagccgcg atcgcctccc ggccaaccgg accggaagtg gcgatgatcg
     4141 cggtatcgcg cgccatcacg cgcgccttcc agtccgcgac atccgccacc gacacggtgg
     4201 cgtagtgctc cggatctatc ctgaggaagt cccggtgcgc gccgggctcc agcaggaacc
     4261 gcaggcgcaa ctgcccggcg atggtgtcgc cgctcgccag tgactggcgg gtgcccagga
     4321 ttcgtgcgcg cctgtgggtt tccagccgcc tttccggaaa gcccggcgct tcgaggaagc
     4381 gcttcagcac gttcgccgcc gccggcaggc tctcgggcgg agccgtgagc gtcagcgtca
     4441 ccgtctcccc cgatgcggca aaccggaagc tggcctgaag atcgctcatc aactcctgga
     4501 tttcaccgcg cttttcgcct ctggggcctt catgcatcat ggccggaccg agagcgctca
     4561 gcgcggcctt gccggccagc gccaggggcg agccatccag ccacaggaac tggatcgtct
     4621 gcatcggcgc ttcgccaagc gggatgcgct tgtaggcgat gcccgcgacc gtccgctccc
     4681 gcaggatgtc gggacctgcc ttgaccggca gggacgccag cacggtcacg acggcggcga
     4741 gcgcgacgcg cctcatggcg ccaccgccgg accgtagatg ccggtgacga tgcggccggg
     4801 tccc
//
LOCUS       DAOMXN010000194         4751 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_9600_length_4751_cov_8.388416, whole genome shotgun sequence.
ACCESSION   DAOMXN010000194 DAOMXN010000000
VERSION     DAOMXN010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4751
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9600_length_4751_cov_8.388416"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="PK812_11660"
     CDS             complement(<1..291)
                     /locus_tag="PK812_11660"
                     /inference="COORDINATES: protein motif:HMM:NF018704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 domain-containing protein"
                     /protein_id="HRJ70251.1"
                     /translation="MAYATKADIEALHGAAFLETLVAADLDLTTAVATACDAASAEMD
                     SYLGQRFPLPLAVAPPILKTWCVDVACWRLAGTAAQMSEEIRKRAERVLAILK"
     gene            complement(336..410)
                     /locus_tag="PK812_11665"
     tRNA            complement(336..410)
                     /locus_tag="PK812_11665"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(376..378),aa:Met,seq:cat)
     gene            complement(505..738)
                     /locus_tag="PK812_11670"
     CDS             complement(505..738)
                     /locus_tag="PK812_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70252.1"
                     /translation="MATKSQSPKAEKPQAFSDAAISVVCTQPGGRRRAGYQFGTEPVV
                     IAVADLDGAAIEALASDPYLSIRGVKPAEAKAE"
     gene            complement(821..1717)
                     /locus_tag="PK812_11675"
     CDS             complement(821..1717)
                     /locus_tag="PK812_11675"
                     /inference="COORDINATES: protein motif:HMM:NF021628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HRJ70253.1"
                     /translation="MIINRGNLTGLTTGFRTNFQNAFDGVTPTWPRVATLVSSNTAAE
                     EYPWLGAFPRMRKWVGDRVVKNLAANGYRIVNEKFEDTVGVKRDSIEDDQFGVYKPMF
                     EGLGRAVAEFPDELVYAKLAKGFTENCYDGQFFFDTDHPVINPATGQAESVSNMQAGV
                     GAPWFLLDTTKAMRPLILQERQKARFTSLDNPNDDHVFKKDEFIYGADCRYGVGFGFW
                     QMAFGSKAALSTANFDAARLAMENVKSDEGVKLGIRPTLLVIGSSNEAAAEAILLKQN
                     LAGGESNVNYKRVEILKVSWLD"
     gene            complement(1758..2156)
                     /locus_tag="PK812_11680"
     CDS             complement(1758..2156)
                     /locus_tag="PK812_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003121593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70254.1"
                     /translation="MALSKERNTYQRDAQQLHLPVAANVKCWQGGLAVIDAGNVKPGV
                     TGLNLIAAGRFEETVDNIGGAAGAAMAPVRRGCFLFGNSGADPVVQADVGKDCFIVDD
                     ETVAKTSGGNTRSRAGVVRGVETTGVWVEI"
     gene            complement(2184..3167)
                     /locus_tag="PK812_11685"
     CDS             complement(2184..3167)
                     /locus_tag="PK812_11685"
                     /inference="COORDINATES: protein motif:HMM:NF021627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HRJ70255.1"
                     /translation="MTTRTPPACLSALPAGLAAGVATVELLRADAGSYPEWIKITPRG
                     EVLTRDRRAYRIDPERLAAAFKADGTKPAVDIGHNTERGLFDSVPPPAIGWVEDMEAR
                     ADGLYARVDWLAAGKAALDSRGYRYVSPCFWTDEDGVNARLVKSVALVTVPALGGLPA
                     LNSATGASPMNPELLAALGLSATATPAEALAVISALKVPDPTKYVPAEQLAAANAELT
                     TARAELKAAKDAEAAARCAALVDDAVRAGKVTPAAKDHYLAFAKADFNACSAALAALP
                     VLVKPGEDDETEAAPAAGNTAATLSADQIKIADSLGIKHADFAAALAAEAA"
     gene            complement(3494..4705)
                     /locus_tag="PK812_11690"
     CDS             complement(3494..4705)
                     /locus_tag="PK812_11690"
                     /inference="COORDINATES: protein motif:HMM:NF016148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HRJ70256.1"
                     /translation="MAGLFATAPKEVIEYFDRRGTRVSWRWTDFAPHEHALGFTVART
                     EGYDVIDTLREAVRKAVVDRVPYETFRDELIPKLKEKSWWGTIRRGGEKVELGSLRRL
                     QTIYWANIRSAHAAGEWARIQQTKDFLPFLQYRRTLSERARPEHLGWVGITLPVDDPW
                     WRTHFPPNGWNCKCRVEQIGEVAAGRAPPDRRIRPRLETDLWRDKTSGRTQRVPKGID
                     PGWQRNPGVTREVVAAALEREKLGFLSHATRSTAIADHRAGAVFRYVAGNGASLPWPM
                     PPLRERSPELMARARLMTPVASAPDSGPAAPPAGVVQISGEDAAKLQGKHRLSVTDFG
                     VVQVLLDHADARLRDADGKWKLAAEINGRFWLAVIKQTSNNEVLLNSLHRITRGQYAA
                     LVARAKRDISG"
BASE COUNT          755 a         1573 c         1601 g          822 t
ORIGIN      
        1 cttaaggatc gccagcacgc gttcggcgcg cttcctgatc tcttccgaca tctgcgccgc
       61 cgtgccggcg agccgccagc aggcgacatc gacacaccag gtcttcagga tcggcggcgc
      121 gaccgcgagc ggcaggggaa aacgctggcc gagatagctg tccatctcgg cggaggcggc
      181 atcgcaggcc gtggcgacgg ccgtcgtcag atcgagatcg gccgcgacca gcgtctccag
      241 gaaggccgcg ccatgcaggg cctcgatatc ggctttggtc gcgtaagcca tggctgcctc
      301 gatacgtctg tccctgatgc cggcattgcc ggaagtggtt gcggcggagg gatttgaacc
      361 cccgacctcc agggtatgaa cctgacgggc taccgctgcc ctacgccgca gaaacgttcg
      421 gggaggcggg ctgagcccct acggtcaagc cgtccctcaa aggtgctggg ggccttgcga
      481 gccgtaccag acctccccct ctggctattc cgctttggct tcggccggtt tgacgcccct
      541 gatcgacaga taggggtcgc tggcgagcgc ctcgatcgcc gccccgtcga gatcggcgac
      601 ggcaatcacg accggctccg ttccgaactg gtaaccggcg cgacgacggc cgcccggctg
      661 cgtgcagacg acgctgatcg ctgcgtccga aaaggcctgc ggcttttccg ctttcggcga
      721 ctgggatttg gtagccatga tgtgctcctt cagaaccgtc ggcgcggtgc gcccttgctg
      781 gaccggcggt gcaatgccgc cggtcggaca aaggcgaccg tcagtcgagc cagctgacct
      841 tgaggatttc gacgcgcttg tagttcacgt tgctctcgcc gccagcgagg ttctgcttca
      901 gcaggatggc ctcggccgca gcctcgttgg acgagccgat caccagcagt gtcgggcgga
      961 taccgagctt cacgccctcg tccgatttga cgttctccat agccagtcgc gcggcatcaa
     1021 aattggccgt ggacagggca gccttcgaac cgaaggccat ctgccagaag ccgaagccga
     1081 cgccgtaccg gcaatccgcg ccatagatga actcgtcctt cttgaagaca tggtcatcat
     1141 tcggattgtc gagggaggtg aagcgcgctt tctggcgctc ctgcaggatc agcggccgca
     1201 tggccttggt cgtgtccagc aggaaccacg gcgcgcccac gccggcctgc atgttggaca
     1261 ccgactccgc ctggccggtg gccgggttga tcacgggatg atccgtgtcg aagaagaact
     1321 ggccatcgta gcagttttcg gtgaacccct ttgcgagctt ggcgtagacc agctcgtccg
     1381 ggaattcggc gacggcgcgg ccgaggcctt cgaacatagg cttgtagacg ccgaactgat
     1441 cgtcctcgat cgagtcgcgc ttgacgccga ccgtatcctc gaacttctcg ttcacgatgc
     1501 ggtagccgtt ggccgccagg ttcttgacga cgcggtcgcc gacccatttg cgcatgcgcg
     1561 ggaacgcgcc gagccaggga tattcctcgg ccgccgtgtt cgagctcacc agcgtcgcga
     1621 cgcgcggcca ggtcggggta acgccatcga aggcgttctg gaaattggtg cggaagcccg
     1681 tggtgagccc cgtgagattg cctcgattga tgatcatggc cggttcctgg ctagcgggag
     1741 agaagcgggc gcgtgggtca gatttcgacc cagacgccgg tggtttcgac gccgcgcacc
     1801 acgccggcgc gcgagcgggt gttgccgccg ctggtcttgg cgaccgtctc gtcgtcgacg
     1861 atgaagcagt ccttgccgac atcggcctgg acgacgggat cggcgccgga attcccaaac
     1921 aggaagcagc cgcgccgcac cggagccatg gccgcaccgg ccgcaccgcc aatattgtcg
     1981 accgtttcct cgaaacgccc ggcggcgatc aggttgagac ccgtcacgcc cggtttgacg
     2041 ttgccggcgt cgatgacggc gagaccgccc tgccagcatt tgacgttggc ggcgaccggc
     2101 aggtgcagct gctgcgcatc ccgctggtag gtgttgcgtt ccttggacag cgccatgggg
     2161 tcgatccttg tgttgagctt cggtcaggct gcttcggccg cgagcgcggc agcaaagtcc
     2221 gcgtgcttga tgccgaggct gtcagcgatc ttgatctggt cggccgacag cgtggcagcc
     2281 gtgttgccgg ccgcaggggc ggcctcggtc tcgtcgtcct cgccgggctt caccagcacc
     2341 ggcagggcgg ccagcgccgc cgaacaggca ttgaaatcgg ccttcgcgaa agcgaggtag
     2401 tgatccttgg ccgccggggt gaccttgccg gcgcgcacgg cgtcgtcgac cagggccgca
     2461 caccgggcgg cggcctcagc gtccttcgcc gccttcaact cggcgcgcgc agtcgtcagc
     2521 tcggcgttgg cggcggccaa ctgctcggcc ggcacgtatt tggtcgggtc cggcactttg
     2581 agggcgctga tgacggcgag cgcttcggcc ggcgtcgcgg tcgcggagag gccgagagcg
     2641 gctaggagtt cgggattcat cggggatgct ccggttgcgg aattgagagc aggcaggccg
     2701 ccgagagccg gcacggtgac cagcgccacg gatttcacga ggcgggcgtt gacgccgtcc
     2761 tcgtcggtcc agaagcaggg cgagacgtag cgatagccgc gtgaatcgag cgccgccttg
     2821 ccggcggcga gccagtcgac gcgggcatag aggccgtcgg cgcgcgcctc catgtcctcg
     2881 acccagccga tggcgggcgg cggcacgctg tcgaacaggc cacgctcggt gttgtggcca
     2941 atgtcgaccg ccggcttggt tccgtccgcc ttgaaggctg cggccaggcg ctcgggatcg
     3001 atgcgatagg cgcgccggtc gcgggtcagg acctcgccgc gcggagtgat cttgatccac
     3061 tcgggatagg acccggcatc ggctcgcaac agctcaaccg tcgcgacacc ggcggcaagg
     3121 ccggcgggca gcgcagaaag acatgcaggg ggcgttcgcg tcgtcatgcc gcgaccttgg
     3181 cggtgcggcg ctggccctgg catggtgacg ggcgtcaccg gctccggtct ggcgggacaa
     3241 cacggtcagc ttgcccggct tcgccctcga ccgcaagccc cgtgcgcggg atgccggttg
     3301 ccgggctttc gaaggccttc gaagcccttc gaagattttt tacggcccga caccgccaaa
     3361 cgcgatcttc gcgctgtggg gcgatttgtg gggcttgcaa atgtcgaggg tgaagaacgg
     3421 gcggaaggac gcaatccctt cacggcacat gaccggctgt gctagctcgc ccgcgaagga
     3481 cgagatagca tgatcacccg gaaatgtcac gcttcgccct cgcgacaagc gctgcatatt
     3541 gccctcgcgt gatccggtgc aggctgttga gcaggacctc gttgtttgac gtctgcttta
     3601 tgaccgccag ccagaatcgg ccattgatct ccgcagcgag tttccacttt ccgtccgcgt
     3661 cgcgaaggcg cgcatcagca tgatcgagca gaacctggac gactccaaaa tccgtcacgg
     3721 acagccggtg tttgccctgc agcttcgccg catcctcgcc gctgatctgg accacgccgg
     3781 ccggcggcgc ggccggaccg gaatccgggg ccgatgccac cggcgtcatc agcctagccc
     3841 ttgccatcaa ctccggcgac cgctccctga gcggcggcat cggccaaggc aaactcgcgc
     3901 cattgccggc gacatagcga aagaccgcac cggcccgatg atcggcgatc gccgtcgagc
     3961 gggttgcatg cgacagaaat ccaagcttct cccgctccag cgccgccgcc acgacctccc
     4021 gcgtcacgcc ggggttgcgc tgccagcccg gatcgatgcc cttgggcacg cgctgcgtcc
     4081 ggccgctcgt cttgtcacgc cagagatcgg tctccagcct tggccggatg cgcctgtccg
     4141 gaggcgcgcg gccagcggcg acctcgccga tctgctcgac ccggcatttg cagttccagc
     4201 cgttgggcgg gaaatgcgtg cgccaccacg gatcgtcaac gggcagcgtg atgccgaccc
     4261 agcccagatg ctccggacgc gcccgctccg acagggtgcg cctgtattgc aggaagggca
     4321 ggaaatcctt cgtctgctgg atgcgcgccc attcgccggc ggcatgggcc gagcggatgt
     4381 tggcccagta gatcgtctgc aaccggcgca gcgacccgag ctcgaccttt tcgccgccgc
     4441 gcctgatcgt gccccaccag gacttctcct tcagcttggg gatcagctcg tcgcggaagg
     4501 tctcgtaggg cacgcggtcg accaccgcct tgcgcacggc ctcgcgcagc gtgtcgatca
     4561 cgtcgtagcc ctcggtgcgg gcgacggtga agccgagcgc atgctcatgc ggggcaaaat
     4621 cggtccagcg ccaggacacg cgcgtgccgc gccggtcgaa atattcgatc acctcctttg
     4681 gcgcggtggc gaacagcccg gccatgtcag gagccggagc gcccgagccc gtagcctttc
     4741 agcgccgtga t
//
LOCUS       DAOMXN010000195         4719 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_9700_length_4719_cov_11.909520, whole genome shotgun sequence.
ACCESSION   DAOMXN010000195 DAOMXN010000000
VERSION     DAOMXN010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4719
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9700_length_4719_cov_11.909520"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..268)
                     /locus_tag="PK812_11695"
                     /pseudo
     CDS             complement(1..268)
                     /locus_tag="PK812_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008936288.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
     gene            633..818
                     /gene="rpmF"
                     /locus_tag="PK812_11700"
     CDS             633..818
                     /gene="rpmF"
                     /locus_tag="PK812_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003601373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HRJ70257.1"
                     /translation="MAVPKRKTSRMKQGHRRSHDALVAPTYVEDKDSGELRRPHHIDL
                     KTGMYRGRQILKVKTEA"
     gene            1169..2908
                     /locus_tag="PK812_11705"
     CDS             1169..2908
                     /locus_tag="PK812_11705"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HRJ70258.1"
                     /translation="MQFQPESGRDLDPKDRTGSSSAALDARSILSSVGEAVYSWDIES
                     DRITWSQNAVDVLGIAEMAQISSGAGYAVVTDSASPTSLQSTIFECQRKDKGTGVPYT
                     ARYLINPAPGRRVWVEDKGRWFANAQGRPWLAHGVVRVVALPSETEARITDTRQVDPA
                     TGALTRPDFCNAVGEALERMRKDKRNFVLLICAIEDLAHHNKTYGYNVGDELIAAIAR
                     RIRSHIRKLDGIGRYAGNKLGVLLCPSLPDEMERTATRIAHVIRDAPIETSAGTIFAQ
                     VRIGGVAAPRDAGETSEVLAAAEEALAEAKASTTQIFAAYTKDSERVRTRRANVAASD
                     EVLAALNDRRIILAYQPIVRARTRDVAMYEALVRMRGLSGELLGAGRIVPMAEKLGIV
                     HLLDHRVLELAVGALRQHQDLRLTINAGVSTAMHPEWLPALRSQLAACPDVAPRLVIE
                     ITETSLIDDLAGASRMVESIKSLGARVAIDDFGAGHTSFRNMRMLPIDILKIDGAFIK
                     NLTQSSDDRFFVRTLLQLAGHLKIETVAEWVQDEESAKLLADWGVAYLQGDHIGEPIL
                     DIPASRMPGRAVA"
     gene            complement(2915..3472)
                     /gene="phaR"
                     /locus_tag="PK812_11710"
     CDS             complement(2915..3472)
                     /gene="phaR"
                     /locus_tag="PK812_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HRJ70259.1"
                     /translation="MAQEKPAHVIKKYANRRLYHTGTSAYVTLEDLAAMVKKGEDFVV
                     NDAKTGEDITHSVLTQIIFEQESKGQNLLPVNFLRQMIRFYGDSMQSLVPRYLEFSMD
                     RLMQEQQSFRDQMAKAFGGFPLGPLEDQVRVNMTMFNEALRMFTPFAPAKEAPTAGKA
                     GERDDEVSALKRELNAMKARLDKLG"
     gene            3728..>4719
                     /locus_tag="PK812_11715"
     CDS             3728..>4719
                     /locus_tag="PK812_11715"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012652869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HRJ70260.1"
                     /translation="MSRSDIVITGAARTAVGSFGGAFGDVPAHTLGAEAIKAAMARSK
                     VEGGEVGEVILGQILTAGQGQNPARQAAMAAGIPQEVTAFGINQLCGSGLRAVALGMQ
                     QIANGDADIVVAGGQESMSQAQHAAYLRGGVKMGELKMQDTMLRDGLWDAFQGYHMGT
                     TAENVAARWQLTREEQDRFAVASQNKAEAAQKAGKFKDEIVPVTIKTRKGDVVVDADE
                     YIRHGATLDNMAKLRPAFSKDGTVTAGNASGINDGAAAVVLMTAKNAEKRGLTPMARI
                     VSWATCGVDPAIMGTGPIPASRKALERAGWKIGDLDLVEANEAFAAQALSVNKDL"
BASE COUNT          852 a         1564 c         1535 g          768 t
ORIGIN      
        1 gggtcagcag gccatctccg gccaggatgg cgcttgcctc gtcgaaggcc cggtgcacgg
       61 tcggcttgcc gcgacggatg tcgtcgtcgt ccatcgccgg caggtcgtca tggatcagcg
      121 aatagcaatg cacgaattca agcgccgccg cgacctgacg cggcccctcg ccttcgatcc
      181 cgcaaagccg ggcgctttcg aggaccagaa agggccgcag ccgcttgcca cccgccagaa
      241 cgccatgccg catcgcctcg acaagccggg acgactggcc ccggccgtga tgccggatca
      301 gcacgtccag catgtccgag aaccgcgcgg ccccggctcc tgcccaacga tccaaagctc
      361 caggcgattt cgccatgtcc agcgctcccc gtttccgtgt atccgggcgc ccggccggga
      421 gcaacgcgga aagggcgaga gaccggtcag agaaatcggc gccggagggc tagattttcc
      481 gggccgttgt cgctatacgg cccgcttcgc tggggcgtta ccgacgcctg cagggcctta
      541 aggcttcgcc ccggaaacag aattccaccg tttttcagtg gcatggaaag gcgcaggacg
      601 cctggtcagg ccacggttcg ccggagagtg acatggccgt tccgaaaaga aagacgtccc
      661 gcatgaagca gggacatcgc cgctcgcatg atgcgctcgt ggcaccgacc tacgtcgagg
      721 acaaggattc cggcgaactg cgccgtccgc accacattga cctgaagacc ggcatgtatc
      781 gcggccggca gattctcaag gtcaagaccg aggcctgatt acccaggcat cgcgcggtca
      841 cacacgccga tcgaagcggg cggtgcctcg ggcagcgccc gttttttttg tgcccggcca
      901 cggcaggatg atcggctcgg ccggggctgc gatccggtcg cgcagaagtt gcgccacaaa
      961 ggccggcgct ggcgacgcgc accgccgcgg cgtccagtcc cccgccgtct tgtgccgaac
     1021 tggtacagat ctcgccgcca tgacgcgacg ggagcaggat cgggaccatt agcgacgggt
     1081 ttacctgttg caggcaaaat tggcagcgcg atcggtagcg acacactgcg tgcgcgatgc
     1141 gttcgacaag acggcacagg tggcacgcat gcagtttcag ccagaatccg gtagagacct
     1201 cgatcccaag gatcggacgg ggtcctcgtc ggctgcactc gacgcgcggt cgatcctgtc
     1261 gtcggtcgga gaagcggtct attcctggga catcgagagc gaccgcatca cctggtccca
     1321 gaacgcggtc gacgtgctcg gcatcgcgga aatggcgcag atctcgtccg gagcgggcta
     1381 tgcggtggtg accgactcgg ccagcccgac atcgctgcag tcgaccatct tcgagtgcca
     1441 gcgcaaggac aagggcaccg gcgtgcccta cacggcccgc tacctgatca accccgcgcc
     1501 cggacgtcgc gtctgggtcg aggacaaggg acgctggttc gccaacgcgc aggggcgtcc
     1561 ctggctggcg catggcgtcg tgcgcgtcgt ggcgctgccg agcgaaacgg aagcgcgcat
     1621 cacggatacc cgccaggtcg atccggcgac cggagcgctg acccgtcccg atttctgcaa
     1681 cgccgtgggc gaggccctgg agcggatgcg caaggacaag cgcaacttcg tgctgctgat
     1741 ctgcgccatc gaggatctgg cgcatcataa caagacctac ggctacaacg tcggcgacga
     1801 actcatcgcc gccattgcgc gccgtatccg ttcccatatc cgcaaactcg atgggatcgg
     1861 ccgctacgcc ggcaacaagc tgggcgtgct gctgtgcccg agcctgccgg acgagatgga
     1921 acgcaccgcg acgcgcatcg cccatgtcat cagggatgcg ccgatcgaaa ccagcgccgg
     1981 gacgatcttc gcgcaggtcc gcatcggcgg cgtggccgcg ccccgggatg cgggcgagac
     2041 ctcggaggtg ctcgccgccg ccgaggaggc gctggccgag gccaaggcca gcacgacgca
     2101 gattttcgcc gcctacacca aggacagtga gcgcgtcagg acgcgtcgcg ccaacgtcgc
     2161 cgcgtcggac gaggtgctcg ccgccctgaa cgaccgacgg atcatcctgg cctatcagcc
     2221 gatcgtccgc gcgcggacac gcgatgtcgc catgtatgag gcactggtgc gcatgcgtgg
     2281 actttcggga gaactcctgg gggccggacg catcgttccc atggccgaaa agctgggcat
     2341 cgtgcatctg ctggatcacc gcgttctgga actggcggtc ggcgccctgc gccagcacca
     2401 ggacctgcgc ctgaccatca acgccggcgt gtcgacggcg atgcatccgg agtggctgcc
     2461 ggccctccgc agccagctcg ctgcctgccc ggatgtcgcg ccacgccttg tgatcgagat
     2521 caccgagacg tcgctgatcg acgatctggc cggcgcgagc cgcatggtgg aaagcatcaa
     2581 gtcactcggc gccagggtcg ccattgacga tttcggcgcc gggcacacct ccttccgcaa
     2641 catgcggatg ctgccgatcg acattctcaa gatcgatggc gccttcatca agaacctgac
     2701 ccagtcgagc gacgatcgct tcttcgtgcg cacgctgctc cagctcgccg gccacctcaa
     2761 gatcgagacc gtcgcggagt gggttcagga tgaggaatcc gccaagctcc tggcggactg
     2821 gggcgttgcc tatctgcagg gcgatcacat cggcgagccc atcctcgaca tcccggcctc
     2881 caggatgccg ggccgcgcgg ttgcctgatc gaccttaacc gagcttgtcc agccgcgcct
     2941 tcatggcgtt cagctcgcgc ttgagcgccg agacctcgtc gtcgcgctcg ccggctttcc
     3001 cggcggtcgg agcctccttg gcgggggcga acggggtgaa catccgcagc gcctcgttga
     3061 acatcgtcat gttgacgcgg acctgatctt ccagcgggcc gagaggaaac ccgccgaagg
     3121 ccttggccat ctggtcgcgg aacgattgct gctcctgcat cagccggtcc atggagaatt
     3181 ccagatagcg cggcaccagc gactgcatgc tgtcgccgta gaaccggatc atctggcgca
     3241 ggaagttcac gggaagcagg ttctgtccct tgctttcctg ctcgaagatg atctgcgtga
     3301 gcaccgaatg cgtgatgtcc tcgcccgtct tggcgtcgtt gacaacgaaa tcctcaccct
     3361 tcttgaccat ggcggcgagg tcttccagcg tcacataggc gctcgtcccg gtatgataga
     3421 ggcgccggtt ggcgtatttc ttgatgacgt gggcgggctt ctcttgagcc atggcgatat
     3481 ccgctgccgt tccaatggcg ggcgaatgat gccgcaccgc atcataagca tatcagcaat
     3541 gcgtgcgctt ggggaactcc cctgacgatt caaggctaat agacgctcct gaaaggcgct
     3601 cgtgccgcag ggaagaccaa atgcttaatc gtgaaggctt gacggaaggt atcttccccc
     3661 gttttgtcat cgcgcgattt ttgcactgca caccggtgcg gccgccagga aacccaagga
     3721 gagaagcatg tcccgcagcg atattgtgat caccggagcg gcccgtaccg ccgtcggcag
     3781 ctttggcggc gccttcggcg acgttcccgc ccacaccctc ggcgccgagg cgatcaaggc
     3841 cgcgatggcc cgctcgaagg tcgagggcgg cgaggtcggc gaggtgatcc tgggccagat
     3901 cctgaccgcc ggccagggcc agaacccggc tcgccaggcc gccatggccg cgggcatccc
     3961 gcaggaagtg accgctttcg gcatcaacca gctctgcggc tcgggcctgc gtgcggttgc
     4021 gctcggcatg cagcagatcg ccaacggcga cgccgacatc gtggtggccg gtggccagga
     4081 atccatgtcg caggctcagc atgccgccta tctgcgcggc ggcgtcaaga tgggcgaact
     4141 gaagatgcag gacacgatgc tgcgcgacgg cctgtgggat gccttccagg gctaccatat
     4201 gggcacgacc gcggagaatg tcgccgcgcg ctggcagctg acacgcgagg aacaggaccg
     4261 tttcgccgtc gcctcgcaga acaaggccga agccgcccag aaggccggca agttcaagga
     4321 cgagatcgtc ccggtcacca tcaagacccg caagggcgac gtggtcgtcg acgccgacga
     4381 gtatatccgc cacggcgcga cgctggacaa catggccaag ctgcgcccgg ccttctccaa
     4441 ggacggcacc gtgacggccg gcaacgcctc cggcatcaac gacggcgccg ccgccgtggt
     4501 cctgatgacc gccaagaacg ccgagaagcg tggtctcacc ccgatggcgc gcatcgtttc
     4561 ctgggcgacc tgcggcgtcg atccggcgat catgggcaca ggcccgatcc cggcttcgcg
     4621 caaggcgctg gagcgggccg gatggaagat cggcgatctc gatctcgtcg aggccaatga
     4681 ggctttcgcg gcgcaggccc tgtccgtgaa caaggacct
//
LOCUS       DAOMXN010000196         4682 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_9802_length_4682_cov_10.857143, whole genome shotgun sequence.
ACCESSION   DAOMXN010000196 DAOMXN010000000
VERSION     DAOMXN010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4682
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9802_length_4682_cov_10.857143"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="PK812_11720"
     CDS             complement(<1..478)
                     /locus_tag="PK812_11720"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRJ70261.1"
                     /translation="MPIIMEEFAPSQTLPEAVDVAIIGGGIIGITAALELAERGVSVA
                     VCEKGQVGHEQSGRNWGWVRVMGRDPGEIPMALDSQRLWETMAARVGADVGFTRSGIV
                     YVADDEAALARHEEWLSHAEPFQLGTRLLRKSELSDVLPGMTRSFAGALFAALDARA"
     gene            639..1928
                     /locus_tag="PK812_11725"
     CDS             639..1928
                     /locus_tag="PK812_11725"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018095976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRJ70262.1"
                     /translation="MPASPLPIHREPCGWNRLLPSRTPRPAAQGRMTARFAVIGAGYT
                     GLAAARRLAELAPEADVLVLEGTEVGEGSSGRNSGFANPRDSKIGLSVTEMERAERIN
                     AFTAEGFSALLDQMALHGFDCDLEKVGRITGAATALGMQKVQSMIAGATAHGFEHIAL
                     DQAGMRAVTGSDYYQCGIRTAEGYLLQPAKLVRGLADSLPANVRLCENTPVLGLERGK
                     GWTLRTPDALIAVETVVLAANPAIKHFGYWKDGLVTIYTFAGLSEPMSDADCAKLGEP
                     SWGLLPAHRLGSTLRRVGRDRFLVRSLYSYEKPAASRTVRDTLTSAFHRRFPDLKHVR
                     LEHVWGGTTALTMNGSPRWGRIDEQLYGSAGCNGAGIVKGTILGQRLAEMIVTGDPQT
                     ELVTCYGSASSIAPEPFRTIGFHIVSALERRKCGLEA"
     gene            complement(2053..2367)
                     /locus_tag="PK812_11730"
     CDS             complement(2053..2367)
                     /locus_tag="PK812_11730"
                     /inference="COORDINATES: protein motif:HMM:NF019590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HRJ70263.1"
                     /translation="MDLRIYTCKPGRVDDFVAIYKEKAWPLQQKYLGRCLGWYTTREG
                     RLNTVVHLWGYDSQADREARRAAMAADPAFKAYLDAVAAADVLVEMENRLVTPTSFSP
                     VQ"
     gene            complement(2436..3692)
                     /locus_tag="PK812_11735"
     CDS             complement(2436..3692)
                     /locus_tag="PK812_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020180083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HRJ70264.1"
                     /translation="MTTSLPNLVINPQRLWDSLMETARFGATDKGGIKRLTLSDEDRQ
                     VRDWFRRACEAVGCSVAVDECGNMFARRPGRRNDLAPICMGSHLDTQPTGGKFDGVLG
                     VLGALEVLRTMHDLGYETNAPIEVVNWTNEEGSRFAPAMLASGVFAGAFTRDYAYARQ
                     DRDGKRFDAELERIGYRGTEPVGERRIGAMFELHIEQGPILEAENTMIGVVTGVQGVR
                     WYEVTVRGQAAHTGATPMHLRKNALLGAAQMVEEINRIALRHAPDAVGTVGLMEVRPN
                     SRNVVPGEVFFSVDFRHKDDGVLQQMENAFRAALPGVMDPLGLSFEETMIWHSPAVAF
                     HPDLIACVRAGARRAGYGTRDMVSGAGHDAAYVARIAPTTMIFVPCAGGVSHNEAEST
                     SLAECGAGAQVLLEAVLAYDQRFAPG"
     gene            complement(3833..>4682)
                     /locus_tag="PK812_11740"
     CDS             complement(3833..>4682)
                     /locus_tag="PK812_11740"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HRJ70265.1"
                     /translation="RFDVWPLSQIAIAARNPLSLATDGTTPSLWAAIPNLTFVGSDVF
                     RVLMVDLQSMTLVPWTRGLVEGATHRGAYQYLVAPHIWSLGVEIIFYAIAPFAVRLST
                     GRMILLLMALCALQFSIHWMANGRLGWFHLLAPYCLCYFVTGMVAHRVWPRVEGHVGT
                     FARQALMVLPFLLWAFWQVIPMSGATTWVLWLLFAAALPCLFQATKNSRLDARISAYS
                     YPVYLCHTLFTWPMLRLGEWAGIAALALSVGLSWLLIRFIDEPVEAWRQRRAARTGPA
                     ASGSRS"
BASE COUNT          862 a         1593 c         1460 g          767 t
ORIGIN      
        1 cggcgcgcgc gtccagcgcc gcgaacagcg cgccggcgaa ggaacgggtc atgccgggaa
       61 gcacatccga cagttcggac ttgcgcagca ggcgcgtgcc gagctggaag ggttcggcat
      121 ggctgagcca ctcttcatgg cgtgccagcg ccgcctcgtc gtccgcgaca tagacaattc
      181 cgctgcgggt aaagccgaca tccgcgccaa cgcgggccgc catggtctcc cacagcctct
      241 gggagtcgag cgccatgggg atttcgcccg gatcgcgacc catgacgcgc acccagcccc
      301 agttgcgacc cgactgttcg tgcccgacct gccccttctc gcagaccgca acggaaacgc
      361 cgcgttcggc cagttcgagg gccgccgtga tgccgatgat gccgccgccg atgatcgcga
      421 cgtccacggc ctcgggcagc gtctgcgaag gagcaaattc ctccatgatg atcggcatgg
      481 cgttcctttc gtgccggtca gccttatgcc atgacagtag cggactgacc ggacgcgtca
      541 cgcaaaaaca tccggaagat catcgcgggg tgcaccggcc gtttgcatgc ctgcgggaat
      601 gtgatcgagt aggctttcat cgacaggacc gcacgcccat gcccgcatcg ccccttccca
      661 ttcatcgcga accctgcggc tggaaccggc tcctgcctag ccgcacgccg cgacctgcgg
      721 cgcagggacg catgacggcg cgctttgccg tcatcggcgc cggctacacg ggactggcag
      781 ccgcccgcag gctcgccgaa cttgcacccg aggcggatgt tctcgtgctc gaaggcaccg
      841 aggtcggaga aggctcgtcc gggcgcaact cgggcttcgc caatccccgc gattcgaaga
      901 tcggcctgtc cgtcaccgag atggagcgcg ccgagcgcat caatgccttc acggcggaag
      961 gcttttccgc gctgctcgat cagatggcgc tgcatggctt cgactgcgac ctcgaaaagg
     1021 tcggacggat caccggcgcg gcaacagcgc tgggcatgca gaaggtgcag tccatgatcg
     1081 ccggcgcgac ggcgcacggc tttgaacaca tcgccctgga tcaggccgga atgcgcgcgg
     1141 tgacgggcag cgactactac caatgcggca tccgcacggc cgaaggctat ctgctccagc
     1201 cggccaagct cgttcgtggg ctcgccgaca gcctgccggc gaatgtgcgg ctgtgcgaga
     1261 atacccccgt cctcggtctt gaacgcggca aaggctggac cctgcgcacc ccggacgcgc
     1321 tgattgcagt ggaaaccgtc gtgctcgccg ccaaccccgc gatcaagcac ttcggctatt
     1381 ggaaagacgg tctggtcacg atctacacct tcgccggcct cagcgaaccg atgtcggatg
     1441 cggattgcgc gaagctcggc gaaccgtcct ggggcctgct gcccgcgcac cggctggggt
     1501 ccaccctgcg ccgcgtcggc agggaccgtt ttctcgtccg ctcgctctat tcctacgaga
     1561 agccggctgc cagccgcacg gtccgggaca cactgaccag cgccttccat cgccgtttcc
     1621 cggacctgaa gcatgtccgc ctcgaacatg tctggggcgg caccacggcg ctgacgatga
     1681 acggctcgcc gcgctggggg cgcatcgatg aacagctcta tggatcggcc ggctgcaacg
     1741 gtgcgggcat cgtgaaaggc accatcctcg ggcaaaggct ggccgagatg atcgtcacgg
     1801 gcgacccgca gacggaactg gtcacctgct atggcagcgc cagcagcatc gcacccgaac
     1861 ccttccgcac catcggattc cacattgtct ccgccctcga gcggcgcaaa tgcgggctgg
     1921 aagcctagag cgatatccga tcagattgaa ccatttcggc ggagcgaccc cggcggagcg
     1981 atagatccgg gcgcatgctc gcgccctatc cggaaccgcc gtgcactctt gatgccgcgc
     2041 cgtttccgcg catcactgga cgggcgaaaa gcttgtcggc gtcaccaggc ggttttccat
     2101 ctccaccagc acgtcagccg ctgccaccgc gtccagatag gccttgaagg cgggatcggc
     2161 ggccatcgcc gcacgccttg cctcgcggtc ggcctggctg tcatagcccc acaggtgcac
     2221 caccgtattg agccgccctt cccgcgtcgt gtaccaaccc aggcagcggc caaggtactt
     2281 ctgttgcaaa ggccaggcct tctccttgta aatggcgacg aaatcgtcga cgcggccggg
     2341 tttgcaggtg taaattctca agtccacgat cattcccgtc tcctatcctg tgaagtgcgg
     2401 cgcctccggc gtccgcagag ctgcccggag cgagatcacc ccggagcgaa ccgctgatcg
     2461 taggccagca cggcttcgag cagcacctgc gcgcccgccc cgcactcggc caacgacgtt
     2521 gattccgcct cattatggct gacgccgccg gcgcagggca cgaaaatcat cgtcgtcggc
     2581 gcgatgcgcg cgacataggc cgcgtcatga ccggcgccgg agaccatgtc gcgcgtcccg
     2641 tagccggccc tgcgcgcccc ggctcggacg caggcgatca ggtcgggatg aaacgccacg
     2701 gccggggaat gccagatcat cgtttcttcg aaggaaaggc cgagcgggtc catcactccg
     2761 ggcagcgcgg cgcggaacgc gttttccatc tgttgcagaa cgccatcgtc cttgtggcgg
     2821 aagtccacgc tgaagaagac ctcccccgga acgacgttgc gggagttcgg ccgcacttcc
     2881 atcaggccga ctgtgccaac ggcgtctggc gcgtgcctca gggcgatccg gttgatctcc
     2941 tcgaccatct gcgccgcgcc caggagcgcg ttcttgcgaa gatgcatcgg cgtcgcgccg
     3001 gtatgggccg cctgcccccg caccgtgact tcgtaccagc gcacgccctg gacgccggtc
     3061 acgacgccga tcatcgtgtt ctcggcctca aggatcggac cctgctcgat atgcaactcg
     3121 aacatcgcgc cgatcctgcg ctcgccgacg ggctcggttc cccggtagcc gatgcgttcg
     3181 agttcggcgt cgaagcgctt gccgtcccgg tcctgccgcg cataggcgta gtcccgcgtg
     3241 aaggccccgg cgaagacgcc ggacgccagc atcgccggcg cgaagcgcga gccttcctcg
     3301 ttcgtccagt tcacgacctc gatcggcgcg ttggtctcgt agccaaggtc gtgcatggtg
     3361 cgcagcacct cgagcgcgcc caggacgccg agaacgccat cgaacttgcc acccgtcggt
     3421 tgtgtgtcga ggtgcgatcc catgcagatc ggcgcgaggt cgttcctgcg gcctggccgg
     3481 cgggcgaaca tgttgccgca ctcgtcgacg gcgacggagc accccacggc ttcgcaggcc
     3541 ctgcgaaacc agtcacggac ctgcctatcc tcgtccgaaa gcgtcaggcg cttgatgccg
     3601 cccttgtccg tcgcgccgaa ccgcgccgtc tccatcaggc tgtcccagag ccgctgcggg
     3661 tttatgacga gattgggcag gctggttgtc atggcacgaa atccagtttt gctcgtccgg
     3721 gcgcggcgct gtcctcgagc cgctagcaat aaggtgcgcg ttcagctccg catcaagggg
     3781 cgtacccgca gtgtccacct tcaagggcgc actccatctt ccgcgccgtg ggtcatgatc
     3841 gtgaaccgga agccgctggc ccggtgcgtg cggcgcgtct ctgcctccag gcttcgacag
     3901 gttcgtcgat gaaccggatc agcagccagg acaggccaac cgaaagggcg agcgcggcaa
     3961 tgccggccca ctcgccgagc ctgagcatgg gccaagtgaa cagggtatga cacagataga
     4021 cgggatagga gtaggcgctg atccttgcat ccagccggga gtttttcgtc gcctgaaaca
     4081 ggcaaggcaa cgcggcggcg aaaaggagcc agaggaccca tgtcgttgca cctgacatcg
     4141 ggatcacttg ccaaaatgcc caaaggagaa atggaagcac catgagtgct tgccgcgcaa
     4201 aggtccccac atggccttcg acacgcggcc agacccgatg agcaaccatg cctgtcacaa
     4261 aatagcaaag gcaatagggc gccagcaggt gaaaccagcc cagacggcca ttcgccatcc
     4321 agtgaattga aaactgcagt gcgcatagcg ccatcagcaa caggatcata cgtccggtgg
     4381 ataaccgaac cgcgaagggc gcgatcgcat aaaagatgat ctccaccccc agcgaccaga
     4441 tgtgcggcgc cacgagatac tgatacgcgc cacggtgggt cgcgccctca accagtcccc
     4501 tcgtccaggg caccagggtc atcgactgaa gatcgaccat cagcacccgg aagacgtcag
     4561 agccaacgaa ggtcagattg ggaatcgcag cccacagact tggcgtcgtc ccgtcggtcg
     4621 cgaggctgag cgggtttctt gccgcgatgg cgatctgtga caagggccag acatcaaacc
     4681 gc
//
LOCUS       DAOMXN010000197         4652 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_9881_length_4652_cov_12.544268, whole genome shotgun sequence.
ACCESSION   DAOMXN010000197 DAOMXN010000000
VERSION     DAOMXN010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4652
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9881_length_4652_cov_12.544268"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1664)
                     /gene="recN"
                     /locus_tag="PK812_11745"
     CDS             complement(<1..1664)
                     /gene="recN"
                     /locus_tag="PK812_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020094135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HRJ70266.1"
                     /translation="MLVQLAIRDIVLIEKLDLAFDSGLSVLTGETGAGKSILLDSLSL
                     ALGARGDGSLVRHGLAQGQVTAVFELPQGHAALRCAMEAGADVDGVLLLRRVQLADGR
                     SRAYVNDQPVGVQALRTIGAALVEIHGQHDERALVSADRHRDLLDAFGGLEPLAAEVR
                     SLSDVWRKAESAYEAKAEQIAAARREQEYLTHVADELTRLAPLPGEEDELATRRQVMM
                     ASEKVAAELAEAQEVIAGAHSPIPLISAMLRRLERRASQAPSLIDPAVKALDAALTAL
                     DEAQGALESSLRAAEYDPRELEQIEERLFALRAAARKHSTHVDDLPALADRFRADLAA
                     IATGEADLAKLDRHRREAEKSYRVRAKALSDARRKAAAKLDAAVTAELPPLKLERARF
                     MTEIASEDTATSANGFDRVEFHVQTNPGTRPGPLMKVASGGELARFMLALKVVLADRG
                     SAPTLVFDEIDSGAGGAVADAIGQRLARLSARTQVLAVTHAPQVASKANRHLLIAKES
                     VERGKRVATRVTMLDAVERREEIARMLSGEAISAEARAAADRLLAGG"
     gene            complement(1677..2561)
                     /locus_tag="PK812_11750"
     CDS             complement(1677..2561)
                     /locus_tag="PK812_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HRJ70267.1"
                     /translation="MLRVSSFAGVSRLAAALVLGLTVSACETLESINPLNAFADKGPK
                     SDIGPDQPAEVLFNDGLARMEKKQYEQAAKKFAELDKQYPYSNWSRKALVLSTFANYE
                     AGEYDDAIASGRRFVQLYPADPDAAYAQYLVGMSYFNQIPDITRDQERAEKALIALDE
                     VVRKWPRSEYAGDAKRRVQVARDQLAGKEMEVGRYYLTRRNYSGAVNRFREVLVRYQT
                     TRHVEEALMRLSEAYMALGIVPEAQTAAAVLGHNFPDSPWYKDAFKLLESGGVGPREN
                     TDSWISKLFKGFQRSVGL"
     gene            complement(2806..3750)
                     /gene="lpxC"
                     /locus_tag="PK812_11755"
     CDS             complement(2806..3750)
                     /gene="lpxC"
                     /locus_tag="PK812_11755"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HRJ70268.1"
                     /translation="MKQTTLGDRIVVDGIGVHSGRPARVVLHPSDANTGYSFLRTGLA
                     NGRERLIDGRYTSVRQTELCTVIGSDCGATVSTIEHLLAALAGLGVDNALIEIDGPEL
                     PILDGSSRIFVDAIDQVGVVPLSANRKVIKVLKPVRIENGRSFAELLPGGNALRLDVE
                     IAFDTPVIGRQSRSFEVSPGRFRAEIASARTFGFMRDVEKLWKAGFALGASLENTVAI
                     GDDSVVNPEGLRFADEFVRHKILDAVGDLALAGAAIRGTYRAFCPGHKLNFMMLQALF
                     ADRTAYAIVDASASRREPTHVGHGELPVSAAAYGPDRG"
     gene            complement(4035..>4652)
                     /locus_tag="PK812_11760"
     CDS             complement(4035..>4652)
                     /locus_tag="PK812_11760"
                     /inference="COORDINATES: protein motif:HMM:TIGR03483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRJ70269.1"
                     /translation="HMAARAQSQVAAPAPTQPVAAEVRPEPARMHGDVKVQPAQPRAA
                     LFQDVLAHDNEPAADPQVDASEAFIPPAPERGVIRPPRLPSAEDFGQPAQNLLRQQAP
                     VEAPQPVETRRMTLMQRLAAVGLGRREESRSPEAQAPVAPPQTVPIQPAPSATHAEYG
                     RRPVAPQGYRPAAGQLDSHGRVANQAGASADEQLEIPAFLRRHSG"
BASE COUNT          844 a         1583 c         1436 g          789 t
ORIGIN      
        1 ccccctgcca gcagccgatc agccgccgca cgcgcctcgg cgctgatcgc ctcgcccgaa
       61 agcatgcggg caatttcctc gcgccgctcc acggcatcga gcatcgtgac ccgcgtggcc
      121 acccgcttgc cgcgctcgac cgactccttc gcgatcagca aatgccggtt ggccttggac
      181 gccacctgcg gcgcatgcgt cacggccaga acctgggtgc gcgccgaaag ccgcgccaac
      241 ctctggccga tggcatccgc gacggcgccg cccgcgccgg aatcgatttc gtcgaacacc
      301 agcgtcggcg ccgagccgcg atccgccagc accaccttca gcgccagcat gaagcgggcg
      361 agttcaccgc ctgacgcgac cttcatcagg gggcccggcc tggttcctgg gttggtctgc
      421 acatgaaact cgacgcgatc aaaaccgttc gccgacgtcg ccgtgtcctc gctcgcgatc
      481 tcggtcataa agcgggcgcg ctcgagtttc agcggcggca gttcggcggt caccgccgcg
      541 tcaagtttcg ccgccgcctt gcgccttgca tcggacagcg ccttcgcccg aactctatag
      601 gacttctccg cctcgcgccg gtggcggtcc aatttcgcaa gatcagcctc gcccgtggcg
      661 atggccgcca gatcggcgcg gaacctgtca gccaacgcgg gcagatcgtc cacgtgtgtg
      721 gaatgcttgc gcgcagccgc tcgcagcgca aaaaggcgct cctcgatctg ctccagctcg
      781 cgtggatcgt actcggccgc ccggagcgac gactccagcg cgccctgggc ctcgtcaagc
      841 gctgtcagcg ccgcgtccag cgccttcact gccggatcga tcagcgacgg agcctgcgag
      901 gcgcgccgct ccagccgtcg cagcatggcg gaaatcagcg ggatcggcga atgtgccccg
      961 gcgatgacct cctgcgcttc cgcgagttcc gccgcgacct tctccgaagc catcatcacc
     1021 tgccggcgcg tggcaagctc gtcttcctca ccgggcagcg gggcgagcct tgtcagttcg
     1081 tccgcgacat gggtcaggta ctcctgctcg cgccttgcgg cagcgatctg ctccgccttt
     1141 gcctcgtagg ctgattccgc cttgcgccag acgtcgctca ggctgcgcac ctcagccgcc
     1201 agcggttcca acccgccgaa cgcgtcgagc aggtcgcggt gccgatcagc gctgaccagc
     1261 gcgcgttcgt catgctgccc atggatctca accagcgccg cgccgatggt gcgcagtgcc
     1321 tgcacgccaa ccggctgatc gttgacatag gcacggctgc gtccgtccgc caactgcacg
     1381 cgccgcagga ggagaacacc gtccacgtca gcgccggcct ccatcgcgca ccggagcgca
     1441 gcatggccct gcggcaattc gaaaacggcc gtgacctgtc cttgagccag gccatggcgg
     1501 accaggctcc cgtcgccgcg cgcgccgagg gcaagcgaca gcgaatcgag caggatggat
     1561 ttacccgcgc ctgtctcccc ggtgagcacg ctcaggccgg aatcaaaggc aaggtcgagt
     1621 ttctcgatga gaacgatgtc gcggatggca agctggacca gcatgccttc cgaatatcag
     1681 agtccgacgg agcgctggaa gcccttgaac agcttgctga tccaggaatc ggtattttcc
     1741 cttggaccga caccgccgga ttcgaggagc ttgaaggcgt ccttgtacca cgggctatca
     1801 gggaagttgt gtcccagaac agcggctgcg gtctgcgctt ccggcacgat gccgagcgcc
     1861 atgtaggctt cggaaaggcg catcagagcc tcttccacat ggcgcgtggt ctggtagcgc
     1921 accagaacct cgcggaagcg attgaccgct cccgaatagt tgcgacgggt gaggtagtag
     1981 cgccccactt ccatttcctt gccggcgagc tgatcgcggg ccacctgcac gcggcgcttg
     2041 gcatcaccgg cgtattcgga gcgcggccat ttgcgcacaa cctcgtcgag cgcgatcagc
     2101 gccttttccg cccgctcctg atcacgcgtg atgtcgggta tctgattgaa atacgacatg
     2161 cctacgagat actgggcgta ggccgcatcc ggatcggcgg gatacaactg cacgaagcgg
     2221 cgacctgagg cgatcgcatc atcatattcg ccggcctcgt agttcgcgaa ggtggagagc
     2281 accaacgcct tccgcgacca gttggaatag ggatactgct tgtccagttc ggcaaacttc
     2341 ttcgccgcct gctcgtactg cttcttctcc atgcgggcga ggccgtcgtt gaacagcact
     2401 tccgccggct gatccggacc aatgtcgctc ttggggccct tatcggcgaa ggcattgaga
     2461 ggattgatgc tttccagcgt ctcacacgca gacaccgtca ggccgagcac aagcgcggcc
     2521 gcgaggcgtg aaacaccggc aaaagatgaa acccgcagca tacctgaaca cctatccaag
     2581 atcggcgtga cccttgcacg agaaatttgg caagcgcaac tcgttcaccg ggctgtgcgc
     2641 caaccccctg gtaacgatta attaacatcg ttaacgcccg catcgccgga aaatgtggcc
     2701 atccgtaggc ggatcatccc ggaagccgcg acgaaagccc gtgtgttgcc agaatgcaaa
     2761 aaggccccgg catcgaccgg ggcctcggct cggcgcaacc cgggatcacc cccggtcagg
     2821 cccataggcc gcggcggaaa ccggcaattc gccatgcccg acatgcgtcg gctcccgacg
     2881 cgatgccgag gcgtcgacga tcgcataggc cgtgcgatca gcaaaaagcg cctgcaacat
     2941 catgaaattg agcttgtggc ccggacagaa cgcacgatac gtgccgcgga tggcggcacc
     3001 ggcgagagcc agatcaccga ccgcgtcgag aatcttgtgg cgcacaaact cgtcggcgaa
     3061 gcgcagccct tcgggattga ccacgctgtc atcaccgatc gcgaccgtat tctccagcga
     3121 agcgccgaga gcgaagcccg ctttccagag cttctcgacg tcgcgcatga aaccgaaggt
     3181 gcgcgccgac gcgatctcag ccctgaaccg gcccggtgaa acctcaaagc tacgggactg
     3241 gcggccaatg accggggtat cgaacgcgat ctcgacatcc agtctcaggg cattgccacc
     3301 aggcagcagt tcggcgaagg agcgcccgtt ctcgatgcga accggcttga gaaccttgat
     3361 gaccttgcgg ttggcggaaa gcggaaccac gccgacctga tcgatcgcat cgacaaaaat
     3421 ccgggaactt ccgtcaagga tggggagttc cggtccatca atctcaatca gcgcgttatc
     3481 gacgcccaac cccgccagag cggcaagcag atgctcgatg gtggacaccg tcgcaccgca
     3541 atcggagccg atgaccgtgc acaactcggt ctgccgcacc gatgtatacc ggccgtcgat
     3601 cagccgctcc cggccattgg ccagacccgt gcgcaagaag ctgtacccgg tattcgcgtc
     3661 cgacggatgg agaaccacgc gggcaggccg cccggaatgg acgccgatac cgtccaccac
     3721 aattcgatcg ccaagcgttg tctgcttcac ttgccccgtc cttcgcccag gtgccggatg
     3781 acctccggct tccccgacac ttcttggcgc cacgggggcc ttaccgcaag gcaaagcggt
     3841 ccccgaccac gcgactacgc tatggtgaat acccgcgtaa cggccgggag caaaatcacg
     3901 ctttctaacg ctctgtaaca caaatccgcc gcgcattcgc gcgacggatt tctgccattt
     3961 ccctgtatat ttacaatagg ttggagccag tcgcatgggc gttgtgccac actttggtcc
     4021 aaatcttcga aacgtcagcc ggagtgccgg cgcaggaaag cggggatttc cagttgctcg
     4081 tccgcgctgg cgcccgcctg gttcgccacc cggccgtgcg aatcgagttg tccggctgcg
     4141 ggacgatagc cctgcggggc caccgggcgc cggccgtatt cggcatgggt cgcggaaggc
     4201 gccggctgga tcgggaccgt ctgcggcggc gcaaccggag cctgcgcttc aggagagcgg
     4261 gactcttccc tgcgcccgag gcctacggcc gcaaggcgtt gcatcagcgt catgcgacgc
     4321 gtttcgactg gctggggcgc ttctacaggc gcctgctgac gcaggagatt ctgtgccggt
     4381 tgcccgaagt cctcggccga aggcaagcga ggcggacgga tgacgccacg ttccggcgcc
     4441 ggcggaatga aggcctcgct cgcgtcgacc tgcggatcgg cggcaggttc gttgtcgtgg
     4501 gccagcacgt cctggaacag tgcggcacgc ggctgggccg gctgtacctt gacgtcgcca
     4561 tgcatgcgcg ccggctcggg gcgcacttcc gctgcgaccg gctgcgtagg cgcgggcgcc
     4621 gcgacctggc tctgggcgcg tgccgccatg tg
//
LOCUS       DAOMXN010000198         4603 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_10030_length_4603_cov_10.037599, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000198 DAOMXN010000000
VERSION     DAOMXN010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10030_length_4603_cov_10.037599"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2540
                     /gene="mfd"
                     /locus_tag="PK812_11765"
     CDS             <1..2540
                     /gene="mfd"
                     /locus_tag="PK812_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015824276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HRJ70270.1"
                     /translation="YRPLPVDALYVSTAEWQALREAKTTVETTPYALPEGGARWVVPL
                     GAKTGRSFAAERADEKANVFDAVVAHVRDVQKAGKRAIVASFTEGARQRTAGVLADHG
                     LKAAQNVGSLRAALELKPGEAGLAVFGLEQGFVTADFAVISEQDILGERMTRPQRSAK
                     RAKDFIAEVTSLSAGDVVVHVDHGIGRFVGLKTIDVGGAPHDCLEIHYHGGDKLFLPV
                     ENIELLSRYGSEETEVQLDRLGGTAWQARKARLKKRIRDMAKALIKIAAARMLREAPA
                     FGKPEHAFDEFVARFPYTETEDQQDAIDATLEDLARGRPMDRLICGDVGFGKTEVALR
                     AAFVAAMAGKQVAIVVPTTLLARQHFKTVSERFKGLPVRVAQASRLVSAADLKAVRSE
                     LESGTLDIVVGTHALLGKSIRFKDLGLLIVDEEQHFGVAHKERLKELRAEVHVLTLSA
                     TPIPRTLQLALTGVRELSIIATPPVDRLAVRTFVTPFDPLVVREALLRERFRGGKSFY
                     VVPRIDDLAEVKAFLDSNVPEVKVGIGHGQMSPSALEEVMSGFLDGTFDVLLSTTIVE
                     SGLDISTANTLIVHRADMFGLAALYQLRGRVGRSKTRAYSIFTLPVNKPVTPNAEKRL
                     KVLQSLDSLGAGFQLASHDLDIRGAGNLLGEEQSGHIKEVGFELYQQMLEEAVAALKA
                     GIEEPEEEQWSPTIAIGAPVLIPESYIEDLDLRMGLYRRLATLETDQQIEGFAAELID
                     RFGPLPREVELLMKIVAIKALCRRAHIDKVEAGPRGVIIAFRDNAFANPSGLVRFVQE
                     RSPTVKVRPDMRVVFLGDFEDQGRRLTETRRLLSELVAIAEGKRKAG"
     gene            complement(2558..3454)
                     /locus_tag="PK812_11770"
     CDS             complement(2558..3454)
                     /locus_tag="PK812_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015886715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0018576 - catechol 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0009712 - catechol-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HRJ70271.1"
                     /translation="MTTGFAPDALTRSVIETMQRTPDPRLREIMTALATHLHAFVRET
                     RLTEDEFRAATALLNEMGKLASDTHNETVLMAGSLGVSALVCLLNHGENGASETSQNL
                     LGPFWRLNSPRIENGGSLVRSPVAGPSMEATLRFVDHRGEPVAGADVDVWHCSGDGLY
                     ENQDARQVDFNLRGTLTTDLAGEIRFTSIKPIGYPIPTDGVVGRLLKAQDRHAFRPAH
                     VHALAVKPGFKVLISQVYADDDPNLASDPQFGVTPATTGRFERHEAPHPGRPEFSTPW
                     YSLRHTLTMEPGEPRLPKPPIR"
     gene            complement(3451..4194)
                     /locus_tag="PK812_11775"
     CDS             complement(3451..4194)
                     /locus_tag="PK812_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70272.1"
                     /translation="MRGKSIRLSPARRIVSDLSYFARRTPLGVMTIRMNIAPVLAARA
                     AAPDRPPLSALLMKGFALVAARHAPLRQAYVKFPWPHLYEYPASVGGVVVEREVEGEA
                     AIFIARVKNPESRPLSEVAALLANAKSAPLASIKDFRLTLALGRFPLLIRRFVWWLGL
                     NLGRQRANYFPTFLISLLGQGGIGINYPVMPFTLFLSIGPISPEGEVTLTCAFDHRGM
                     DGAVVARGMAELEDALNGPIADELRSAGS"
     gene            complement(4196..>4603)
                     /locus_tag="PK812_11780"
     CDS             complement(4196..>4603)
                     /locus_tag="PK812_11780"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRJ70273.1"
                     /translation="GSAMAARGRGSIVFVSSILGHQPGPVHAYSAAKAGLEALTRSLA
                     TEWGTAGVRVNAVAPGFAETPLLEKATHFGALDADQLTATTALGRLATPDEIANAVAF
                     LSSALASGITGASLAVDAGFLAAAGGRAFPARS"
BASE COUNT          769 a         1488 c         1609 g          737 t
ORIGIN      
        1 cctatcgtcc gctgccggtc gatgcgctgt atgtgtcgac ggcggaatgg caggcgttgc
       61 gcgaagccaa gaccacggtc gagacaacgc cctacgccct gccggagggc ggcgcgcgct
      121 gggtggtgcc gctcggcgcg aagaccgggc gctcctttgc cgccgagagg gcggacgaaa
      181 aggccaatgt cttcgacgcc gtcgtggcgc atgtccggga cgtgcagaag gcgggcaagc
      241 gcgccatcgt cgccagcttc accgagggcg cgcgccaacg cacggcgggc gtgctggccg
      301 atcacgggct gaaggctgcg cagaacgtcg ggtcgctgcg cgccgcgctc gaattgaagc
      361 cgggcgaagc gggcctggcc gttttcgggc tggagcaggg gttcgtgacg gccgacttcg
      421 ccgtcatcag cgagcaggac atcctgggcg aacgcatgac gcgaccgcag cggtccgcca
      481 agcgcgccaa ggatttcatc gctgaagtca ccagcctttc ggcgggcgac gtggtcgtgc
      541 acgttgatca cggcatcggc cgcttcgtgg ggctgaagac catcgatgtc ggcggcgcgc
      601 cgcacgattg cctggaaatc cactaccacg gcggcgacaa gctgttcctt ccggtcgaga
      661 acattgaact gctctcgcgc tacggatcgg aggagaccga ggtccagctc gatcgactcg
      721 gcggcacggc ctggcaggcg cgcaaggcca ggttgaagaa gcgcatccgc gacatggcga
      781 aggcgctgat caagatcgcc gccgcccgca tgctgcgcga ggcgccggcc ttcggcaagc
      841 cggagcacgc cttcgacgag ttcgtcgcgc gcttccccta caccgagacg gaggaccagc
      901 aggacgcgat cgacgcgacg ctggaggacc ttgccagggg ccggccgatg gaccggctga
      961 tctgcggcga tgtcggtttc ggcaagacgg aagtggccct gcgcgcggcc tttgtcgcgg
     1021 cgatggcggg caagcaggtt gcgattgtcg tgccgacaac gctgctggcg cgccagcatt
     1081 tcaagaccgt gtccgagcgc ttcaaggggc tgccggtgcg tgtcgcgcag gcctcgcgcc
     1141 tggtttccgc agcggacctg aaggccgtcc gcagcgagct ggagagcggc actctcgaca
     1201 ttgtcgtcgg cacccatgcg ctgctcggca aatcgatccg gttcaaggac cttggcttgc
     1261 tcattgtcga tgaggagcag catttcggcg tcgcgcacaa ggagcggctc aaggaactgc
     1321 gcgccgaggt gcatgtgctc acgctttcgg cgacgccgat cccgcgcacg ctgcaactgg
     1381 cgctgaccgg tgtgcgcgaa ttgtcgatca tcgccacgcc gccggtggac cggctggccg
     1441 tgcgcacctt cgtcacgccg ttcgatcccc tggtggtgcg cgaagcgctg ctgcgcgagc
     1501 ggttccgggg cggcaagagt ttctacgtcg tgccgcgcat cgatgatctg gccgaggtca
     1561 aggccttcct cgattccaac gtgccggagg tgaaggtggg catcggccat ggccagatgt
     1621 cgccttccgc gcttgaggag gtgatgagcg gcttcctcga cggcacgttc gacgtgctgc
     1681 tctccaccac catcgtcgaa tccggcctcg acatttcgac cgccaacacg ctgatcgtgc
     1741 atcgggcgga catgttcggc ctcgctgcgc tctatcagct gcgcggccgc gtcggccgtt
     1801 ccaagacacg cgcctattcc atcttcaccc tgccggtgaa caagccggtg acgccgaacg
     1861 cggaaaagcg gctgaaagtg ctgcaatcgc tcgacagtct cggcgccggc ttccagttgg
     1921 cgagccacga tctcgacatt cgcggcgccg gcaaccttct gggcgaggag caatccggac
     1981 acatcaagga ggtgggcttc gaactctacc agcagatgct ggaggaggcg gtcgcggcgc
     2041 tgaaggccgg catcgaggag ccggaagagg agcagtggtc gccgaccatc gccatcggcg
     2101 cgccggtgct catcccggaa agctacattg aggacctcga tctgcgcatg ggcctgtacc
     2161 gccgactggc gacgctggaa accgaccagc agatcgaggg ctttgcggcg gagctgatcg
     2221 accgcttcgg cccgttgccg cgcgaggtcg aactcctgat gaagatcgtc gccatcaagg
     2281 cgttgtgtcg ccgggcgcat attgacaagg tcgaggccgg cccgcgcggc gtcatcatcg
     2341 cgttccgcga caatgccttc gccaatcctt cgggcctcgt gcgcttcgtg caggagcgct
     2401 cgccgacggt gaaggtcagg cccgacatgc gcgtcgtgtt cctgggcgac ttcgaggatc
     2461 aggggcgacg gctcaccgag acgcggcgcc tgctctccga actcgtcgcc atcgccgaag
     2521 gcaagcgcaa ggcgggatga ccgccgcgcg tcggggccta tcggatcggc ggcttcggca
     2581 gacgtggttc gcccggctcc atggtcagcg tgtggcgcaa ggaataccac ggcgtcgaaa
     2641 actccgggcg ccctggatgc ggcgcttcgt gccgctcaaa ccggccggtc gtcgccggcg
     2701 tcacaccgaa ttgcggatca gacgcgagat tggggtcgtc gtcggcgtag acttgcgaaa
     2761 tcagcacctt gaatccgggc ttcaccgcca gggcatggac atgcgccggg cggaaggcat
     2821 ggcggtcttg cgccttcaag agcctgccga cgacgccgtc ggtcgggatc ggatagccga
     2881 tgggcttgat cgaggtgaag cggatttcgc cggcgagatc ggttgtcagc gtgccgcgca
     2941 gattgaaatc cacctgccgg gcatcctggt tctcgtagag cccgtcgccg gaacagtgcc
     3001 acacgtccac atccgcgccg gcgaccggtt cgccacggtg atcgacgaat cgcagcgttg
     3061 cttccatcga tggcccggcg acgggagagc ggacgagcga gccgccgttt tcgatgcgcg
     3121 gcgaattcag cctccagaac ggtccgagca ggttctgtga cgtttcgctc gcgccattct
     3181 ccccgtggtt cagcaggcag acgagcgcgg aaaccccgag ggatccggcc atcagcaccg
     3241 tttcattgtg cgtgtcgctc gccagcttgc ccatctcgtt gaggagggcg gtcgccgcgc
     3301 ggaattcgtc ttccgtcagc cgcgtttcgc gcacgaaggc gtgcagatgc gtcgccagcg
     3361 ccgtcatgat ctcgcgcagg cgcggatcag gcgtgcgctg catggtctcg atcacggaac
     3421 gcgtcagcgc atccggggcg aaacctgtcg tcatgaccct gcgctccgca gctcgtccgc
     3481 gatcggaccg ttcagcgcgt cctcaagttc ggccatgccc cgggcgacca ccgcgccgtc
     3541 catcccgcga tggtcgaagg cgcaggtcag cgtgacctcg ccctccggcg agattggtcc
     3601 gatgctgagg aacagcgtga acggcatcac ggggtagttg atgccaatgc cgccctggcc
     3661 gagcagcgag atgaggaagg tcgggaaata attcgcgcgc tggcgaccga ggttcaggcc
     3721 gagccaccag acgaaccggc ggatcagcag cgggaagcga ccgagcgcca gcgtcagccg
     3781 gaagtccttg atcgatgcga gcggcgcgga tttggcgttg gcgagcagcg cggcaacctc
     3841 ggacagcggg cgcgactccg ggttcttgac gcgggcgatg aagatcgccg cctcgccctc
     3901 gacctcgcgc tccaccacga cgccgccgac gctggccgga tactcgtaga ggtgcggcca
     3961 gggaaacttc acataggcct gccgcaacgg cgcatggcgc gccgcgacca gcgcgaagcc
     4021 tttcatcaac aaggcgctca gcggcggccg gtccggtgcc gccgcccgcg ccgcaagcac
     4081 cggagcgatg ttcatgcgga tggtcatgac gccaaggggc gtccggcggg caaaatagct
     4141 caagtcgctg acgatgcggc gcgcgggcga caggcggatc gatttgccgc gcatgtcagg
     4201 atcgcgcggg gaaggcgcgt ccgccggctg cggccaggaa cccggcgtcg acggcaagcg
     4261 aggcgccggt aatgccggag gcgagcgcgg aagacaggaa cgcgacggcg ttggcgatct
     4321 cgtccggtgt cgcgaggcgg ccaagcgcgg tcgttgccgt gagttgatcc gcgtcgagcg
     4381 caccgaaatg cgtggccttt tcgagcagcg gcgtctcggc gaagccgggc gcgacggcat
     4441 tcacacgcac accggctgtt ccccattcgg tggcgaggct gcgggtcagt gcctccagcc
     4501 cggccttggc ggcactgtag gcatgcaccg ggccgggctg atgcccgagg atcgaggaga
     4561 cgaagacgat gctgccccgg ccgcgcgcgg ccatggcgct gcc
//
LOCUS       DAOMXN010000199         4584 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_10084_length_4584_cov_11.978141, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000199 DAOMXN010000000
VERSION     DAOMXN010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4584
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10084_length_4584_cov_11.978141"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="PK812_11785"
     CDS             <1..742
                     /locus_tag="PK812_11785"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HRJ70274.1"
                     /translation="AAHRRGGGAVPRTAAVLVVQAVAGEPVRMGRVDRDRVAPDKGRR
                     DDVSCRLLRSFQDMTRACALFATPIGACALIWGGNGICGVQLPEVDDAAAVARLRRRW
                     PDARADEPSAEAQAAIDEIGALLQGRPHDFMRFRLDRGGITPFNDRVYDQTLAIPPGE
                     TRSYGDIARALGDIGLSRAVGTALGENPLPILIPCHRVMAAKGGTGGFSAPGGLDTKF
                     RLLNIEQAFARPEPNLFGDTPLPLARRQ"
     gene            complement(749..1990)
                     /locus_tag="PK812_11790"
     CDS             complement(749..1990)
                     /locus_tag="PK812_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRJ70275.1"
                     /translation="MDFSYSPKVVELQKRLNAFMDEHIYPNEAAFHHEVEANRAKGNP
                     WKPTRIMEQLKTEARKAGLWNLWLPQSSHGAGLTNLEYAPLCEIMGRSHIAPEAFNCS
                     APDTGNMEVLVRYATPENQEKWLKPLLAGEIRSAFAMTEPAVASSDATNIQSSIKRDG
                     DHYVINGRKWWTSGAPDPRCKILIFMGQTDPANPNVHKRQSMILVPMDTPGVTMVRPL
                     SVFGYDHAPHGHGEMTFENVRVPASNMLLGEGRGFEIAQGRLGPGRIHHCMRTIGVAE
                     RALEDLCKRALARVAFGRSVADQGVTRERIAEARIMIDQARFLTLHAAWKMDTVGNKM
                     AKQEIAMIKVAAPVMAQQVIDWAMQVHGAAGISGDFNLAANFAGIRTLRYADGPDEVH
                     RNQIAKYELERYVRRNSTGKE"
     gene            complement(2029..3093)
                     /locus_tag="PK812_11795"
     CDS             complement(2029..3093)
                     /locus_tag="PK812_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007606077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HRJ70276.1"
                     /translation="MSDQFTGTKPVDPRHAFAAGPLADWMRAHVAGFSGDLVVEQFKG
                     GQSNPTFLVRAGGKAYVLRRKPPGKLLPSAHAVDREFKVISALAGTDVPVAKAHALCE
                     DDTVIGSAFYIMDFVEGRVLWDPSLPGMTRDERSAIFDEMNRVIAALHKIDYKAVGLS
                     DFGREGAYLARQIDRWTKQYRAAETETIEAMDNLIAWLPQNIPAADETAIVHGDYRLD
                     NVIFHKSEPRILAVLDWELSTLGHPLTDFAYHCMAWRMTPGQFRGIVGYDLPGLGIPD
                     ERAYVAAYCGRTGRSGISARDWEVYMVFNMFRLVGILQGITARALQGNASSAQALEMG
                     KRARPLAELAWKNVERLQNA"
     gene            complement(3106..3885)
                     /locus_tag="PK812_11800"
     CDS             complement(3106..3885)
                     /locus_tag="PK812_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRJ70277.1"
                     /translation="MANETRSLFDLAGKVALVTGGSRGLGLQMAQALGEMGAKVAISA
                     RKADELAEAKKHLEAHGIDVLTVTNDLSKLDQIPALVSTVLERFGAIDILVNNAGATW
                     GAPAEDYPDEAWRKVMTLNVDAMFFLSREVGKRSMIPRKAGKIINISSVAGLSGNPPA
                     MTTIAYNTSKGADINFTRALAAEWGKYNINVNAICPGFFPSKMSAGLLEKIGRDVLSA
                     TPLRRFGGEEDLKGVIVFLASEASRHVTGQYIAVDGGACIA"
     gene            complement(3932..>4584)
                     /locus_tag="PK812_11805"
     CDS             complement(3932..>4584)
                     /locus_tag="PK812_11805"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRJ70278.1"
                     /translation="VGKEAEVAALADAAFARFGNVGLLCNNAGVGVSRVTWEHTLADW
                     RWIMDVDLWSVIYGVHFFLPRMMAQGNGGHIVNTASAAGLVSTGGMNAYNVAKHGVVT
                     LSETLFREAQQAKSGVGVSVLCPAWVPTNIHRSGAYRQAEFGKPRPAGELSAQYERRM
                     AEAVTSGKLTAADMARHVFEAVAENRFYVIPHRKLNQVIQLRADDILNQRNPTPWE"
BASE COUNT          734 a         1436 c         1594 g          820 t
ORIGIN      
        1 ggcggcgcat cgccgggggg gcggggcggt accgcgcacg gcggcggttc tggtggtcca
       61 ggcggtggcc ggggagccgg tcaggatggg cagggtggac cgggatcggg tggccccgga
      121 caagggccgg cgcgatgatg tgtcgtgcag acttctccgc agtttccagg acatgacgcg
      181 cgcctgcgcc ctattcgcga cgccgattgg cgcctgcgcc ctgatctggg gtggcaacgg
      241 catctgcggc gtgcaactgc cggaagtgga tgacgcggcg gcggtcgcac gtctacggcg
      301 gcgctggccg gatgcgcggg ccgatgaacc aagcgccgag gcccaggccg cgattgatga
      361 gatcggcgcg ttgctccagg gcaggccgca cgacttcatg cggtttcgtc tggaccgcgg
      421 cggcatcacg cctttcaacg accgcgtcta tgaccagact ctcgccattc cgcccggcga
      481 gacgcgcagc tacggcgaca ttgcccgcgc gctcggtgac atcggcctgt cgcgcgccgt
      541 gggcacggcc ctcggggaaa acccgctgcc gatcctcatc ccctgtcacc gcgtgatggc
      601 ggcgaagggc ggaaccggcg gcttctcggc accgggcggt ctggacacca agttccggct
      661 cctgaacatc gagcaggcgt tcgcccggcc cgagccaaac ctattcggcg atacgccgct
      721 accgttggcg cggcgacagt agtgctgact attccttccc cgtcgagttg cggcggacgt
      781 agcgctccag ttcgtacttg gcgatctggt tgcgatgcac ctcgtccggc ccgtcggcgt
      841 agcgcaatgt gcggataccg gcgaagttcg ccgcgagatt gaagtcgccg gagatccccg
      901 ctgcgccatg cacctgcatt gcccagtcga tcacctgctg cgccatgacc ggcgcggcga
      961 ccttgatcat ggcgatttcc tgcttcgcca ttttgttgcc cactgtgtcc atcttccagg
     1021 ccgcgtgcag ggtgaggaag cgcgcctgat cgatcatgat gcgggcttcg gcgatgcgct
     1081 cgcgcgtcac gccctgatcc gcgacggaac ggccgaaggc gacacgcgcg agggcgcgct
     1141 tgcacagatc ctccagcgcg cgttcggcga cgccgatggt gcgcatgcaa tggtgaatgc
     1201 gtcccgggcc aaggcggccc tgcgcgatct cgaaaccgcg gccctcgccc agcagcatgt
     1261 tggaggccgg cacgcgcaca ttctcgaaag tcatctcgcc atggccgtgc ggggcgtggt
     1321 cgtagccgaa cacggacaat gggcgcacca tggtgacgcc gggcgtgtcc atcggcacca
     1381 ggatcatgga ctggcgcttg tggacgttgg gattggccgg atcggtctgg cccatgaaga
     1441 tcaggatctt gcagcgcggg tccggcgcac cggacgtcca ccacttgcgg ccattgatga
     1501 cgtagtggtc gccgtcgcgc ttgatcgagg actggatgtt ggtggcgtcg gaggaggcga
     1561 cggccggctc ggtcatggcg aaggcggagc ggatttcgcc ggcaagcagc ggcttcagcc
     1621 atttctcctg gttctccggg gtggcgtagc ggaccagcac ctccatgttg ccggtatcgg
     1681 gcgcggagca gttgaaggct tcaggcgcga tatgcgagcg gcccatgatc tcacagagcg
     1741 gggcgtattc gaggttggtt agacccgcgc cgtggctgga ttgcggcagc cagaggttcc
     1801 atagccctgc cttgcgcgcc tccgtcttga gctgctccat gatcctggtg ggtttccagg
     1861 gattgccctt ggcgcggttc gcctcgacct cgtgatggaa cgccgcctcg ttaggataga
     1921 tgtgctcgtc catgaaggca ttgagccgct tctgcagttc gacgaccttg ggggaatagg
     1981 agaagtccat ggcgtgcctc gtggagtctg tgcggatggt gttgcggatc aggcgttctg
     2041 gagccgttcg acgttcttcc aggcgagttc ggccagcggc cgggcgcgct tgcccatctc
     2101 cagcgcctgt gccgaggagg cattgccctg aagcgcgcgc gcggtgatac cctgaagaat
     2161 gccgacgagg cggaacatgt tgaagaccat atagacctcc cagtcgcgtg ctgagatccc
     2221 gctgcgtccg gtgcgcccgc aataggccgc gacataggcc cgctcgtcgg gaatgccgag
     2281 acccggcaga tcatagccca cgatgccgcg gaactggccc ggcgtcatgc gccaggccat
     2341 gcaatggtag gcaaagtcgg tcagcggatg gccgagcgtc gagagttccc agtcgagcac
     2401 ggcgaggatg cgcggctcgg acttgtggaa gatcacgttg tccagccggt agtcgccatg
     2461 gacaatcgcg gtttcgtcgg cggccgggat gttctgcggc agccaggcga tgaggttgtc
     2521 catcgcctcg atcgtctcgg tctcggcggc gcggtactgc ttggtccagc ggtcaatctg
     2581 gcgggcgaga taggcgccct cgcggccgaa gtccgacagg ccgacagcct tgtagtcgat
     2641 cttgtgcagc gcggcgatga cgcggttcat ctcgtcaaag atggcgctgc gctcgtcccg
     2701 cgtcatgccc ggcaggctgg ggtcccacag cacgcggccc tcgacgaaat ccatgatgta
     2761 gaaggccgag ccgatgaccg tatcgtcctc gcagagcgca tgcgccttcg ccacgggaac
     2821 atcggtgccg gccagcgcgc tgatgacctt gaattcgcgg tccaccgcat gggcggaggg
     2881 cagaagcttg cccggcggct tgcggcgcag cacgtaggcc ttgccaccgg cgcgaaccag
     2941 aaacgtcggg ttcgactggc cgcccttgaa ctgctcgacc acgagatccc cggaaaatcc
     3001 ggccacatgc gcgcgcatcc agtccgccag cggacccgca gcgaaagcgt gcctcggatc
     3061 gaccggcttg gttccggtga actggtcgct catggcgctc cccgctcagg cgatgcaggc
     3121 gccgccgtcg acggcgatgt actggccggt gacgtggcgc gaggcttcgg aggccaggaa
     3181 gacgatcacg cccttgaggt cctcctcgcc gccgaagcgg cggagcggtg tcgcagacag
     3241 cacgtcccgg ccgatctttt cgagcaggcc ggccgacatc ttcgaaggaa agaagccggg
     3301 gcagatggcg ttgacgttaa tgttgtactt gccccactcg gcggcgagcg cgcgggtgaa
     3361 gttgatgtcc gcgcccttgg aggtgttgta ggcaatggtg gtcatcgcgg gagggttgcc
     3421 ggacaggccg gcgacggacg agatgttgat gatcttgccg gcttttcgtg ggatcatcga
     3481 gcgcttgccc acttcgcggc tgaggaagaa catggcgtcg acgttcaggg tcatgacctt
     3541 gcgccaggct tcgtccgggt agtcttcggc gggcgccccc caggtggcgc cggcgttgtt
     3601 gaccaggatg tcgatggcgc cgaagcgctc cagcaccgtg gaaaccagcg cgggaatctg
     3661 atcgagcttc gacaggtcgt tggtgaccgt cagcacgtcg atgccgtggg cttcgaggtg
     3721 cttcttcgct tccgccagtt catcggcctt gcgcgcggag atcgccacct tcgcgcccat
     3781 ctcgcccagg gcctgcgcca tctgaaggcc gaggccgcgc gagccgccgg tgacgagcgc
     3841 gaccttgccc gcaagatcaa aaagggagcg tgtttcgttg gccatgattg ccttccttac
     3901 aatgcgccgc gccgcagcga cggcctgacc gtcattccca cggcgtcggg ttgcgctgat
     3961 tgaggatatc gtccgcacga agctgaatca cctggttcag cttgcggtgc gggatgacat
     4021 agaagcgatt ctcggcgacg gcctcgaaga cgtggcgcgc catgtcggca gcggtaagct
     4081 ttccggaggt cacggcctcg gccatgcgcc gctcgtactg ggcggacagt tcgccggccg
     4141 ggcgcggctt tccgaattcc gcctgccggt aggcgccgga gcggtggatg ttggtcggca
     4201 cccaggccgg gcaaaggacc gacacgccga caccggactt tgcctgctgc gcttcgcgaa
     4261 agagggtttc ggacagggtc acgacaccgt gcttggcgac attgtaggcg ttcatgccac
     4321 cggtggacac cagaccggct gccgacgccg tgttgacgat atggccgccg tttccttgcg
     4381 ccatcatgcg cggaagaaag aagtggacgc cgtagatgac gctccacagg tcgacgtcca
     4441 tgatccagcg ccagtcggca agcgtgtgtt cccaggtgac gcgcgagacg ccgacaccgg
     4501 cgttgttgca cagaaggccg acgttgccga agcgggcgaa ggccgcgtcc gcgagcgccg
     4561 cgacctcggc ttccttgcca acgt
//
LOCUS       DAOMXN010000200         4574 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_10108_length_4574_cov_12.556096, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000200 DAOMXN010000000
VERSION     DAOMXN010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4574
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10108_length_4574_cov_12.556096"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..158)
                     /locus_tag="PK812_11810"
     CDS             complement(<1..158)
                     /locus_tag="PK812_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HRJ70279.1"
                     /translation="MPINVLMPALSPTMEKGNLAKWLKKEGDRIKAGDVIAEIETDKA
                     TMEVEAVD"
     gene            complement(162..440)
                     /locus_tag="PK812_11815"
     CDS             complement(162..440)
                     /locus_tag="PK812_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70280.1"
                     /translation="MGPVVFTAIMLLALAAFVTALVFFLRGARCAWNDPGFAPHRARL
                     IWNYMGAIQHAPEEAKVHFRRFYAALAVFIACCIAGVLAGFTLTTTSR"
     gene            complement(453..1496)
                     /gene="pdhA"
                     /locus_tag="PK812_11820"
     CDS             complement(453..1496)
                     /gene="pdhA"
                     /locus_tag="PK812_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HRJ70281.1"
                     /translation="MAVAAKAPKASAHKKAASNGGSAAPAVPEFTRSQDLQAYRDMLL
                     IRRFEERSGQLYGMGLIGGFCHLYIGQEAVVTGMQMAAIPGDQVITGYRDHGHMLACG
                     MDAKGVMAELTGRRGGLSKGKGGSMHMFSKEKHFYGGHGIVGAQVSLGTGLAFANKYR
                     KNNNVAMIYFGDGAANQGQVYESFNMAELWKLPAIYVIENNRYAMGTAVTRASAQTDF
                     SKRGVSFNIPGEQVDGMDVRSVKAAADRACAFARAGNGPYILEMQTYRYRGHSMSDPA
                     KYRTKEEVDQMRAEHDPIDMVRERLLKKKWATEDELKKIDAEVRAIVAESAEFASQDP
                     EPDASELWTDVLL"
     gene            1765..2871
                     /locus_tag="PK812_11825"
     CDS             1765..2871
                     /locus_tag="PK812_11825"
                     /inference="COORDINATES: protein motif:HMM:NF025464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparin lyase I family protein"
                     /protein_id="HRJ70282.1"
                     /translation="MRHILFFGLGLAAAASAASANSLRDNFDKRALDLKNWSAAQINP
                     SQIGFEQPGRCGGAAIRITVKAKDGGSSCDSCQRAEVRVNQQFQPKYGDEMWYAFSFR
                     VSGDVPRDGASRNVIAQFKAPGDDSPFLAQRIDNGVFQITMEDSGVRRVIAKSEGDPE
                     SLGKAQNLLSGYDPSDARGRMVMRNLQAVHRIARTTPDIAGLLFTHDAQSGLRMPAGK
                     DFTSANGAKMSSLFGMEGEDAAVAMREMVAVNDIDRYLGRADITVAPGPGAVLPNPFD
                     GWVDMVYRIKGGRTDNTAGPNHQGEVDIWANGRLVANVRGNFGNKLTATPADTAMYFK
                     FGIYRDARPFEQKVLFDEYAQGTSRSDVTLACAK"
     gene            complement(2930..3244)
                     /locus_tag="PK812_11830"
     CDS             complement(2930..3244)
                     /locus_tag="PK812_11830"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HRJ70283.1"
                     /translation="MVVRTRFRRAMMLLAFHTIVLGASGFFAWHAYTGERGIIARREH
                     KVRIAQLNRQLEELKIERMAHERRVSELAYDELDRDLLDERARVILNLAHRNDLVILT
                     GK"
     gene            3359..3718
                     /locus_tag="PK812_11835"
     CDS             3359..3718
                     /locus_tag="PK812_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70284.1"
                     /translation="MRRSAYSLLLAAILASPAHAQQAGSLPRGLFGENARECQAATNG
                     DENGARAISRDGKVVESFASRCSVRATTRAGATFSLSTTCQGEGQSWAEPIEIVVVNR
                     DTLRWSERGSTRTLHRC"
     gene            complement(3725..>4574)
                     /gene="eno"
                     /locus_tag="PK812_11840"
     CDS             complement(3725..>4574)
                     /gene="eno"
                     /locus_tag="PK812_11840"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRJ70285.1"
                     /translation="MMNIVNGGAHADNPIDFQEFMIMPAGADSFSEGLRWGAEVFHTL
                     KKALKDAGHNTNVGDEGGFAPNLPSAEGALDFVMKAIEKAGFKPGKDMYIALDCASTE
                     FFKKGVYHYEGEGKKRSIDEQVKYLAKLVKGYPIVSIEDGMAEDDWEGWKGLTDAIGS
                     KCQLVGDDLFVTNVKRLSDGIKKGVGNSILVKVNQIGSLTETLAAVDMAHRAGYTAVM
                     SHRSGETEDSTIADLAVATNCGQIKTGSLARSDRTAKYNQLLRIEQELGDTAIYAGRG
                     ALKALR"
BASE COUNT          810 a         1460 c         1438 g          866 t
ORIGIN      
        1 tcatcgaccg cctcgacctc catggtcgcc ttgtcggtct cgatctcggc gatgacgtcg
       61 ccggccttga tccggtcgcc ttcctttttc agccacttgg ccagattgcc cttctccatg
      121 gtcggagaca gcgcaggcat gagaacattg atgggcatcg actacctcga tgtcgttgtc
      181 agggtgaagc cggcgaggac accggcgatg caacaggcga tgaagactgc gagcgcggca
      241 tagaacctgc ggaagtgaac cttcgcttcc tcaggcgcat gctggatggc gcccatgtag
      301 ttccagatca ggcgcgcgcg gtgaggcgcg aagccggggt cgttccaggc gcaccgggcg
      361 ccccgcagga agaagaccaa cgctgtcacg aaggccgcca gggccagaag catgatcgcg
      421 gtgaagacga ccggccccat ggcgcgctgt ccttacagca gcacgtcggt ccagagctcg
      481 gaggcatccg gctccgggtc ctgcgaggcg aattcggcgg actccgccac gatggccctg
      541 acctccgcgt cgatcttctt cagttcgtcc tcggtggccc attttttctt gagcaggcgc
      601 tcgcgcacca tgtcgatggg gtcatgttcc gcccgcatct ggtccacctc ctccttcgtc
      661 cggtatttcg ccggatcgga catggaatgg ccgcgatagc ggtaggtctg catttcaagg
      721 atgtaggggc catttccggc gcgggcgaag gcgcaggcac ggtccgccgc cgccttgacc
      781 gagcgcacgt ccatgccgtc aacctgctcg cccgggatgt tgaaggagac gccccgtttg
      841 gagaaatccg tctgcgccga ggcgcgcgtc accgccgtgc ccatggcgta gcgattgttc
      901 tcgatcacgt agatggccgg cagtttccac aactccgcca tgttgaagct ctcgtaaacc
      961 tgcccctgat tggcggcgcc gtcgccgaaa tagatcatcg cgacgttgtt gttcttgcga
     1021 tacttgttcg cgaaggcgag gcctgttcca agcgagactt gcgcgccgac gatgccatgc
     1081 ccgccgtaga agtgtttctc cttggagaac atgtgcatgg agccgccctt gcccttcgac
     1141 aggccgccgc gacgcccggt gagttcggcc atgacgccct tggcgtccat gccgcaggcc
     1201 agcatgtggc cgtgatcgcg gtatcctgtg atgacctggt cgcctgggat cgccgccatc
     1261 tgcatgcccg tcacgaccgc ttcctggccg atgtagaggt ggcagaaacc gccgatcagc
     1321 cccatgccgt acaactggcc ggagcgctcc tcgaaacggc ggatgagcaa catgtcccga
     1381 taggcctgca gatcctgcga ccgggtgaac tcgggaactg cgggagcggc ggaaccgccg
     1441 ttagaggcgg cttttttgtg cgccgaagct ttcggcgcct tggcggcgac ggccatgcat
     1501 tctccccttt ggtcgtttcc tgtcagcgaa ggtattcgct tcccccgcaa tgacaagacg
     1561 ctgatagaat gactttctct ctccaggcag tggcttgcca acaggcaatc catgcggaaa
     1621 ttgcataacg tcgggccgcc ggcggcctac cacgcatgcg cggcgcgtga tgtggatcgt
     1681 tcaattttta aacaccggtc aaacggaagt ctgaaacttc tgcgctactg ttcgcgtgta
     1741 tgaatggcgg accggagagg gacgatgcgc catatcttgt tttttggact cggacttgcc
     1801 gctgcggcaa gcgcggcaag cgcgaactcg cttcgcgaca acttcgacaa acgcgcgctg
     1861 gacctgaaga actggtcggc ggcgcagatc aacccgtctc agatcggctt cgaacagcct
     1921 ggacgctgcg ggggcgcggc catccgcatt accgtgaagg cgaaggacgg cggctcctcg
     1981 tgcgattcct gccaacgtgc tgaagtccgg gtaaaccagc agttccagcc gaaatacggc
     2041 gacgagatgt ggtatgcgtt ctcgttccgg gtcagcggtg atgtgccgcg cgacggcgcc
     2101 tcgcgcaatg tcattgccca gttcaaggcg ccaggggacg acagcccctt cctggcacag
     2161 cggatcgaca atggcgtctt ccagatcacc atggaagaca gcggcgtgcg ccgcgtcatc
     2221 gccaagtcgg aaggcgatcc ggaatcgctt ggcaaggccc agaacctgct ctccggctac
     2281 gatccctccg atgcccgcgg ccgtatggtc atgcgcaatc ttcaggccgt ccatcgcatc
     2341 gcgcggacaa cgccggacat cgccggcttg ctgttcaccc acgacgcgca gtccggcctc
     2401 aggatgccgg ccggaaagga ctttacgagc gccaacggcg ccaagatgtc gagcctgttc
     2461 ggcatggaag gcgaagacgc cgccgtggcg atgcgcgaaa tggtcgccgt caacgacatc
     2521 gaccgctacc tcgggcgcgc cgacatcacg gtggctccag gtcctggcgc cgtcctgccc
     2581 aatccgttcg atggctgggt cgacatggtc tatcgcatca agggagggcg cacggacaac
     2641 acggccggcc ccaatcatca gggcgaggtc gacatctggg cgaacggcag gctcgtcgcc
     2701 aacgtgcgcg gcaatttcgg caacaagctg accgccacac ctgccgacac cgcgatgtac
     2761 ttcaagttcg gaatctaccg ggatgcccgg cccttcgagc agaaagttct gttcgacgaa
     2821 tacgcccagg gcacgagccg ttctgatgtc acgttggcct gcgccaaata gctgacaagg
     2881 aaagcgggac ggcaggatat caacgcggtc cgcagtgcgg gccgcgtttt catttgccgg
     2941 tcaggatcac caggtcgttg cggtgggcga gattcaggat cacgcgcgcg cgctcgtcca
     3001 gcaggtcccg gtccagttcg tcataggcga gttcgctcac ccggcgctca tgcgccatgc
     3061 gctcgatctt cagttcttcg agctggcggt tgagctgggc aatccgcacc ttgtgctcgc
     3121 gacgcgcgat gatgccgcgc tcgcccgtgt aggcgtgcca ggcaaaaaag ccgctcgcgc
     3181 ccagcacaat ggtgtggaac gccagcaaca tcatcgcgcg acggaatcgg gtgcgaacga
     3241 ccatgcccgc agaattgcgc gcatgggtta aaggatcgta atcaacgccg cccgctgctt
     3301 ggcttgacgg ttgggcatga agcggccttc atgagcgccg caaccttgag gatcatccat
     3361 gcggcgcagt gcctacagct tacttctggc ggcgatcctg gcatccccgg cccatgccca
     3421 gcaggcaggc tccctgccgc gtggcctttt cggcgagaac gcgcgggagt gccaggccgc
     3481 cacgaacggc gacgagaacg gcgcgcgcgc catttcacgg gatggcaagg tggtcgaatc
     3541 cttcgccagc cggtgcagcg tccgcgccac gacgcgcgcc ggtgcgacat tcagtctctc
     3601 cacgacctgc cagggcgaag gtcagtcctg ggcggagccg atcgagatcg tcgtcgtcaa
     3661 cagggacacg ctgcgctgga gcgagcgcgg gtcgacgcgc acgctgcacc gttgctgagc
     3721 gtgatcagcg cagcgccttg agcgcgccgc ggccggcata gatcgcggta tcgcccagtt
     3781 cctgctcgat gcgcagcaac tggttgtact tcgcggtgcg atccgaacgg gccagcgagc
     3841 cggtcttgat ctgcccgcag ttggtggcca ccgccagatc ggcgatggtg gaatcctcgg
     3901 tctcgcccga gcggtgcgac atgacggcgg tatagcccgc gcgatgcgcc atatccacgg
     3961 cggcgagcgt ctcggtgagc gagccgatct ggttgacctt gaccaggatc gagttgccga
     4021 cgcccttctt gatgccgtcc gacaggcgct tgacgttggt gacgaacaga tcgtcgccga
     4081 caagctggca cttggagccg atggcgtcgg tcaggccctt ccagccctcc cagtcatcct
     4141 cggccatgcc gtcctcgatg gaaacgatgg gatagccctt caccagcttg gcgaggtatt
     4201 tcacctgctc gtcgatggag cgcttttttc cttcgccctc atagtgatac acgcctttct
     4261 tgaagaactc tgtggaggcg cagtcgaggg cgatgtacat gtccttgccc ggcttgaacc
     4321 ccgccttctc gatggctttc atgacgaagt cgagcgcacc ttccgccgac ggcaggttcg
     4381 gggcgaaccc gccctcgtcg ccgacgttgg tgttgtggcc cgcgtccttc agcgccttct
     4441 tcagcgtgtg gaacacctcc gcgccccagc gcaggccttc ggaaaagctg tcggcgccgg
     4501 ccggcatgat catgaattcc tgaaagtcga tcggattgtc ggcgtgggcg ccgccgttga
     4561 cgatgttcat catc
//
LOCUS       DAOMXN010000201         4571 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_10116_length_4571_cov_8.900354, whole genome shotgun sequence.
ACCESSION   DAOMXN010000201 DAOMXN010000000
VERSION     DAOMXN010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4571
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10116_length_4571_cov_8.900354"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="PK812_11845"
     CDS             <1..453
                     /locus_tag="PK812_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRJ70286.1"
                     /translation="ARPDAGKAVQTSLRLPFIFLCGTALYLWRDRVRLGWPAALGAAL
                     LAAVLSGTFAYKALMFAAEAYAVLVFALGPLAGKLPEPKADVSYGTYLYGWPVQQALH
                     ATFPEAGERWLLVPALAMTLLIAAASWYLVEKPALRLKPGSGGPSRSE"
     gene            complement(466..1620)
                     /locus_tag="PK812_11850"
     CDS             complement(466..1620)
                     /locus_tag="PK812_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70287.1"
                     /translation="MLRSTPHAFALALATAGAVQAQTAPIGPPVITIDGPDELVYDTK
                     RDGCDGYDVPDVPPRAFRDAAGKVRMVALHFDNRLLKGDSLDRLKLDCRVVFRTSGSG
                     VPEAYDDRSWITATWTRDGKSVHGLVHHEYQGNTHPGRCTKKDYLSCWFNTILAVRSD
                     DSGESFMKATTPVVASAPFPQSVGQGRHRGFFNPSNIVTDGVWHYMIAGTTGWDGQNS
                     GACLFRTDDIANPTRWRAFDGIGFETAFVDPYRAKPNPGLACQTVGPFPAPVGSITRH
                     RPSGAWVAVFQASAGGRFPAAGIYAAASKDLKTWSEARLILPTKTLYDDACGAEALNS
                     YPTLLDRTAETRNFEDTGDTPDLYLSRMRIDGCKHTGDRKLIRMRVKIAF"
     gene            1766..2425
                     /locus_tag="PK812_11855"
     CDS             1766..2425
                     /locus_tag="PK812_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ70288.1"
                     /translation="MSDIFREVDEEFKRDQFAQFLKKHGSLIIGVLLLVVLGVGGWRG
                     YGYYQERRAAEAGGRYDEAIRLASDGKAAEAKAMFEGVLKDAPTGYQQLTKFRLASEL
                     ARSDAAGAIKAFEALAADAGLDPVLRDLARVRAAVLLIDTAPLAEIEQRLQPFAAPGQ
                     AWRLTAREGLGLAAFKAGDAEKASKYLEQILADPEATQTVRQRAEILISMARAGSAPT
                     K"
     gene            2427..3800
                     /gene="der"
                     /locus_tag="PK812_11860"
     CDS             2427..3800
                     /gene="der"
                     /locus_tag="PK812_11860"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HRJ70289.1"
                     /translation="MQPTVVIAGRPNVGKSTLFNRLVGQKLALVDDQPGVTRDRREGD
                     ARLYDLDFTIVDTAGFEDAPAATLAGRMRRQTETAISEADAVLFLIDARAGVTPDDER
                     FAALVRKSGKPVILLANKAEARMAQGGVLDAYSLGLGEPIAFSAEHGEGLADLHSALE
                     AVFERAPVDDAGDEGGDEPDAAEGAGKPLRIAIVGRPNAGKSTLINTLLGEDRLLTGP
                     EAGITRDSIGLQFEWRGRAVKMFDTAGLRRKSRVEEKVEKLAVGDALRAVRFAEVVVI
                     LLDAAIPFEKQDLSIVDLVEKEGRAVVIALNKWDLIADRQKTLADLREKCDRLLPQVK
                     GCALVTVSGLAGEGVDRLMEACFRAADVWSRRISTAQINRWLEGALSAHAPPAVSGRR
                     IKIRYMTQPKARPPHFALFGNQLSALPDAYRRYLVNGLRETFDLPGTPIRLSMRTSDN
                     PYGKKGR"
     gene            complement(3891..>4571)
                     /locus_tag="PK812_11865"
     CDS             complement(3891..>4571)
                     /locus_tag="PK812_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015952194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRJ70290.1"
                     /translation="DLDFLTVHGDPHVVAAARTTGALEELDDAIRRRGGSATLVPIDL
                     RDLDAVDRFAPALQQRWGKLDILIGNAGALGPISPVPHIDPKTFDEVFKVNVAANYRL
                     LRSLDPLLRASDAGRAIFISSGAARRVRAYWGLYAASKAALEALVRAYAAEVMNISNV
                     RAMLVNPGPIRTAMRAAAMPGEDPDTLPPPEAIVPDILRLASPDWSETGKLFDFPTKS
                     VIGFDEPK"
BASE COUNT          729 a         1454 c         1634 g          754 t
ORIGIN      
        1 gcccggcccg acgccggaaa ggcggtgcag acgtcgttgc gcctgccgtt catcttcctg
       61 tgcggaacgg cactctacct gtggcgtgac agggtgcgcc tcggctggcc ggctgcgctc
      121 ggcgcggcgc tgcttgccgc ggtcctgagc ggtaccttcg cctacaaggc cctgatgttc
      181 gcggccgagg cctatgcggt tctggtcttc gccctcggtc cgctggccgg gaaactgcct
      241 gagccgaagg cggatgtctc ctacggcacc tatctctacg gctggccggt gcagcaggcg
      301 ttgcatgcga cgttcccgga ggccggcgaa cggtggctgc ttgttccggc gctcgcgatg
      361 acgctgctga tcgccgccgc ctcgtggtac ctcgtcgaga agcctgcgct gcgattgaag
      421 cctggcagcg gcggtccatc gcggtcagag tgacgctctc gccaatcaga acgcgatctt
      481 gacccgcatg cggatcagct tcctgtcgcc ggtgtgcttg cagccgtcga tccgcatgcg
      541 gctgaggtag agatcaggcg tgtcgccggt gtcctcgaag ttgcgggttt ccgccgtgcg
      601 atccagcaaa gtcgggtagg aattcagcgc ttcggcgccg caggcgtcat cgtagagggt
      661 tttcgtcggc aggatcagcc gggcctcgct ccaggttttc aggtccttcg aggccgcggc
      721 gtagatgccg gccgcaggaa aacggccacc cgccgaggcc tggaacacgg cgacccaggc
      781 gccgctgggg cgatgcctgg tgatcgatcc caccggagcg ggaaaggggc cgacggtctg
      841 gcaggcaagg cccggattgg gcttggcccg gtagggatcc acgaaggccg tctcgaagcc
      901 gatcccgtcg aaagcgcgcc agcgggtggg attggcgata tcgtcggttc tgaacaggca
      961 ggcgccgctg ttctgtccgt cccagccggt ggtgccggcg atcatgtagt gccagacgcc
     1021 gtcggtgacg atgttcgacg ggttgaagaa gccgcgatgc cggccttgtc cgacgctctg
     1081 cggaaagggc gcgctggcga cgacaggcgt ggtggccttc atgaagctct cgccggaatc
     1141 atcggagcgg acggccagaa tggtgttgaa ccagcaggac agataatcct tcttcgtgca
     1201 gcggccggga tgggtgttgc cctgatactc gtgatggacc aggccgtgga cgctcttgcc
     1261 gtcgcgcgtc caggtcgcgg tgatccagct gcggtcgtca taggcctccg gcacgccgga
     1321 ccccgaagtg cggaacacca cgcgacaatc cagtttcaga cggtcgaggc tgtcgccctt
     1381 gaggaggcgg ttgtcgaagt gcagcgcgac catgcggacc ttgccggcgg catcgcggaa
     1441 ggcgcggggc ggcacatccg gcacgtcgta gccgtcacag ccatcccgct tggtatcgta
     1501 gaccagttcg tccgggccat cgatggtgat gaccggcggt ccgatcggcg ccgtctgggc
     1561 ctgaacagcg ccggcggtcg ctagcgccag ggcgaaggca tggggggtcg atctcagcat
     1621 ggtcgcttcc agccgctcca aagggccgcc agctaagcac agctgcgtta atggctcgca
     1681 atcggccccg ggctgtgata gcgggggcgc ctgttttcag cttgcggaca tggcttccgg
     1741 gcgccgcccg gaagaggacg ggcagatgtc ggatatcttt cgcgaagtcg acgaggaatt
     1801 caaacgcgac cagttcgcgc agttcctcaa gaagcacggc agcctcatca tcggcgtgct
     1861 gcttctggtt gtgctcgggg tcggcggctg gcgcggctac ggctattatc aggagcgccg
     1921 tgccgcggag gccggcgggc gttacgacga ggcgatccgg ctggcttcgg acgggaaggc
     1981 ggccgaagcg aaggccatgt tcgagggcgt gctgaaggac gcgccaacgg gctatcagca
     2041 gctgacgaag ttccgtctcg cttcagaact ggcgcgttcc gacgcagccg gagcgatcaa
     2101 ggctttcgag gcgctggcgg cggatgccgg cctcgatcct gtgctgcgcg acctcgcccg
     2161 ggtgcgcgcc gcggtgctgt tgatcgacac ggccccgctg gccgagatcg agcagcggct
     2221 gcaacccttc gcggcgcccg gtcaggcctg gcgactcacg gcgcgcgagg gcctcggcct
     2281 ggcggccttc aaggcgggcg acgcggaaaa agcctcgaag tatctcgaac agatcctggc
     2341 cgatcccgag gcgacacaaa ccgttcgcca gcgcgccgaa atcctgatct cgatggcgcg
     2401 ggcgggctct gccccgacga agtgacatgc aaccgactgt cgtcattgcc ggccggccga
     2461 atgtcggcaa gtcgacgctg ttcaaccgtc tggtcgggca gaagctggcg cttgtcgacg
     2521 atcagccggg tgtaacccgc gaccgccgcg agggcgacgc gcggctctat gacctcgact
     2581 tcaccatcgt cgacactgcg gggttcgaag atgctccggc ggccacgctg gccgggcgca
     2641 tgcggcggca aaccgaaacg gcgatctctg aagcggatgc cgtgctgttc ctgatcgatg
     2701 cgcgggccgg cgtgacgccg gacgacgagc gctttgccgc cctggtgcgc aagagcggca
     2761 agccggtcat cctgctcgcc aacaaggccg aggcgcgcat ggcgcagggc ggcgtgcttg
     2821 atgcctactc gcttggcctc ggcgagccga tcgccttctc tgccgagcat ggcgaggggc
     2881 tggctgacct gcactccgca ctggaagcgg tgttcgagcg cgcgcccgtg gacgacgccg
     2941 gagacgaggg gggcgacgag ccggacgccg ccgagggggc aggcaagccc ctgcgcatcg
     3001 ccatcgtcgg gcggcccaat gccggcaagt ccacgctgat caacacgctg ctcggcgagg
     3061 accggctgct gaccggaccc gaggcgggga tcacgcgcga ttccatcggc ttgcagttcg
     3121 agtggcgcgg cagggcggtg aagatgttcg acaccgccgg cctgcggcgc aagtcgcgtg
     3181 tcgaggagaa ggtcgagaag ctggcggtgg gcgatgcgct gcgcgccgtc cgtttcgcgg
     3241 aagtcgtggt gatcctgctg gatgccgcca ttcctttcga gaagcaggac ctgtccatcg
     3301 ttgacctcgt ggagaaggaa gggcgcgcgg tggtgatcgc cctcaacaag tgggacctga
     3361 tcgcggaccg ccagaagacg ctggcggacc tgcgcgagaa atgcgaccgc ctgctgccgc
     3421 aggtgaaggg ctgcgcgctt gtcaccgttt ccggcctggc aggcgagggc gtggaccggc
     3481 tgatggaggc ctgcttccgc gcggcggacg tgtggagccg ccgcatttca acggcgcaga
     3541 tcaatcgctg gctggagggg gcgctgtcag cccacgcgcc gccggcggtt tcgggacggc
     3601 gcatcaagat ccgctacatg acgcagccga aggcgcgccc gccgcatttc gcgctgttcg
     3661 gcaaccagct ctcggccctg ccggacgcct atcgtcgcta cctcgtcaat ggcctgcgcg
     3721 aaacgttcga tcttcccggc acgccgatcc gcctgagcat gcggaccagc gacaacccct
     3781 acggaaagaa ggggcggtag acccgtcggg cgtcaattcg tggcgctccc ttgggagcga
     3841 acccggaaag acagtcggaa gcgtcgtttc cgccctctgc gattcctgcc ctatttcggc
     3901 tcgtcgaagc cgatcaccga cttcgtcggg aagtcgaaga gcttgccggt ctcgctccag
     3961 tccggcgatg ccagccgcag gatgtccggg acgatggcct cgggcggggg cagggtgtcc
     4021 gggtcctcgc ccggcatggc ggcggcgcgc atggcggtgc ggattgggcc gggattgacc
     4081 agcatggcgc gcacgttcga gatgttcata acctctgcgg cataggcccg caccagcgct
     4141 tccagcgcgg ccttggaggc ggcgtaaagg ccccagtagg cccgcacgcg ccgcgccgcg
     4201 cctgaggaga tgaagatggc ccgccccgcg tccgaggcgc gcagcagcgg atcaagcgag
     4261 cgcagcaggc ggtagtttgc ggcgacgttg accttgaaca cctcgtcgaa ggtcttcggg
     4321 tcgatgtgcg gcaccggcga aatggggccg agcgctccgg cattgccgat cagaatgtcc
     4381 agcttgcccc aacgctgctg caacgccggc gcaaatcggt ccaccgcatc gagatcgcgc
     4441 aggtcgatcg gcaccagcgt ggcagagccg ccgcgcctgc ggatcgcgtc gtcgagttcc
     4501 tcaagcgcgc cagtcgtgcg ggcggcggcg acgacatggg ggtcgccatg gacggtcagg
     4561 aaatcaaggt c
//
LOCUS       DAOMXN010000202         4571 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_10117_length_4571_cov_8.179584, whole genome shotgun sequence.
ACCESSION   DAOMXN010000202 DAOMXN010000000
VERSION     DAOMXN010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4571
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10117_length_4571_cov_8.179584"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..1196
                     /locus_tag="PK812_11870"
     CDS             162..1196
                     /locus_tag="PK812_11870"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HRJ70291.1"
                     /translation="MTIWAVRAGKYGEGEKFALDNSCVVVGWDQMPDLKPLDTRQKMR
                     DAVEQAHADISDKLASNWTGQLWAFRSEIKAGDLIVLPLKGTGTVAVGEALGRYEFRS
                     DASLHGRHTQALRWLDVSLSRARIDSDILYSLGSTLTVFRVQRAHAEERLRALLGAKG
                     GAPARPAGYADVSLASDEAEIDLRTSALDQIRLRIAQRFKGHRLANLVDAVLRAKGFE
                     TKLSLPGADGGVDILAAQGFFSEGAVSLVVQVKSEDRSTDAPTVRQLIGTMDKFQAAR
                     GLFVSWGGFKSSVEREFERDFFRLQFWTSDKLIEEILSTYDRFDESLKALLPLERVWM
                     LVQDAEDEEP"
     gene            1193..2434
                     /gene="coaBC"
                     /locus_tag="PK812_11875"
     CDS             1193..2434
                     /gene="coaBC"
                     /locus_tag="PK812_11875"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HRJ70292.1"
                     /translation="MTLASKRILLIIGGGIAAYKSLDLIRRLRERGALVRCVLTRAGT
                     EFVTPLSVSSLSGDKVYQDLFSLTDEAEMGHIELSRDADLLVVAPATADLMAKMAQGL
                     ASDLASTLLLATDKRVLLAPAMNVRMWLHPATRRNVEMLRRDGALFVGPNEGAMACGE
                     FGPGRMAEPLEIVAAIESALAMPTLLPLPGAPQQIAGPKPLAGKHVLITSGPTHEPID
                     PVRYIANRSSGKQGHAIAAAAVAAGARVTLVSGPVTVADPSGASVVRVESARDMLAAV
                     EKALPADIAIFAAAVADWRVDHVAGEKIKKGGSGPPRLALVENPDILATVCRSTRRPA
                     LVVGFAAETEKVIEHARAKRARKGCDLIVANDVSPETGIMGGDRNTVHLVGPDSVETW
                     PDLPKDEVARRLVAALAERAP"
     gene            2431..2889
                     /gene="dut"
                     /locus_tag="PK812_11880"
     CDS             2431..2889
                     /gene="dut"
                     /locus_tag="PK812_11880"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003614550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HRJ70293.1"
                     /translation="MSLVLPLKILPHGAGLPLPAHATPGAAGMDVCAAVDTPVTLEPG
                     ARALIPCGIAIAVPEGHEAQLRPRSGLALKHGVTVLNAPGTIDSDYRGEVKALLINHG
                     HEPFVIERGMRIAQIVIAAVARVRVTVVPALDDTARGAGGYGSTGGIGQS"
     gene            3015..3989
                     /gene="cysK"
                     /locus_tag="PK812_11885"
     CDS             3015..3989
                     /gene="cysK"
                     /locus_tag="PK812_11885"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006205370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HRJ70294.1"
                     /translation="MSTMTNAERRPGRGRVYSSITETIGDTPLVRLDRLTKAKGIEAT
                     LVAKLEFFNPISSVKDRIGVAMIEAMERDGRIAPGRTTLIEPTSGNTGIALAFVAAAR
                     GYRLILTMPESMSIERRKMLTYLGAELVLSEAGKGMKGAIAKAEELAATIPGAIIPQQ
                     FQNPANPEIHRRTTAEEIWNDTNGAVDVFIAGVGTGGTITGVGQVLKARKPALRVVAV
                     EPAESPVLSGGAPGSHRIQGIGAGFVPEILDVSVIDEVVTIDSQAAIDTARLLARTEG
                     VPGGISAGAATAAALQVAARPDMKGKTIVFIVPSFAERYISSLLFEGL"
     gene            4016..4432
                     /locus_tag="PK812_11890"
     CDS             4016..4432
                     /locus_tag="PK812_11890"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRJ70295.1"
                     /translation="MPTGFDDLELESIVAGHTMLSKLKRNWPAAFPPHASLLVAVEDG
                     PMLLGIGGIAPDPYDVEPGRGRIRHLYVAHAARRLGVGRRILNSCLDFARSRFHTVSL
                     RTPASEQANRFYEAHGFRPLHGHANASHVLYLPRQP"
     gene            4495..4571
                     /locus_tag="PK812_11895"
     tRNA            4495..4571
                     /locus_tag="PK812_11895"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4529..4531,aa:Arg,seq:ccg)
BASE COUNT          785 a         1454 c         1525 g          807 t
ORIGIN      
        1 ctgctgacgc agctggaagt cgctgcggcg cagggcttcc ggctgtcgga cgcctcggcg
       61 gaagccatcg gccgcgcgga agcccgtcgc gcgcgctggg gcaacgcggc gctgtgggtg
      121 atggctgggt tgcttgccta ctgggtcttg ggaggcggtg gatgacaatc tgggctgtca
      181 gggcgggcaa atatggcgaa ggcgagaaat tcgcgctcga caacagttgc gtggtcgtcg
      241 gctgggacca gatgcccgat ctcaaaccgc tggatacaag acagaagatg cgagatgccg
      301 tggagcaggc tcacgcggac atctctgaca aattggcttc aaactggacc ggccagctct
      361 gggcctttcg ctccgaaatt aaggccggcg acctgatcgt gctgcccctc aagggcacgg
      421 gcacggtcgc tgtcggagaa gcactcgggc ggtatgagtt ccggtccgat gcgtcgctgc
      481 acgggcgtca tacgcaggca ttgcgctggc tggatgtgtc gctgtcccga gcccgcattg
      541 acagcgacat tctctattcg ctcggatcaa ctctgacagt tttccgcgtt caacgcgctc
      601 atgcggaaga acgcttgcgc gctcttcttg gagctaaggg cggcgcgcct gccagaccgg
      661 ctggttatgc cgatgtttct ttggcttccg atgaggccga aatcgacctc cggacatcgg
      721 cgcttgacca aatccggttg cgcatcgccc agcgcttcaa ggggcaccgg ctcgcaaatc
      781 tggtcgatgc cgtgcttcgc gccaagggct tcgaaaccaa gctcagtctt cccggggcgg
      841 atggcggcgt cgacattctg gcggcacagg gtttcttcag cgaaggcgcg gtgagtctcg
      901 tggtgcaggt gaagtccgaa gaccggtcaa cagacgcccc gaccgttcgt caactcattg
      961 gaacgatgga caagtttcaa gcggcgcgtg gattgttcgt gtcctggggc gggttcaaat
     1021 catcggttga gcgggagttc gagcgggatt tctttcgatt gcagttctgg accagtgaca
     1081 agctgatcga agaaatcctt tcgacctacg atcgtttcga cgaaagtttg aaagcgcttc
     1141 tcccgctcga acgagtctgg atgctggttc aagatgcgga ggacgaagaa ccatgaccct
     1201 cgcttccaaa cgcatcctgc tcatcatcgg cggcggcatc gccgcctaca agagccttga
     1261 cctgatccgc cgcttgcgcg agcgcggcgc cctggtgcgc tgcgttctca cgcgggcggg
     1321 aacggagttc gtcacgcccc tgtcggtgtc gagcctgtcc ggtgacaagg tctatcagga
     1381 cctgttctcg ctcaccgacg aagcggagat gggccatatc gaattgtcgc gcgacgccga
     1441 cctgctggtc gtcgccccgg cgacggccga tctgatggcg aagatggcgc agggccttgc
     1501 cagcgatctg gcctcaaccc tgttgctcgc caccgacaag cgcgtgctgc tggctccggc
     1561 catgaacgtc cggatgtggc tgcatcccgc gacgcggcgc aatgtcgaga tgctgcggcg
     1621 cgacggcgcg ctgttcgttg gcccgaacga gggggcgatg gcctgcggcg agttcggccc
     1681 cggccgcatg gccgagccgc tggaaatcgt cgccgcgatt gaatcggcgc tcgccatgcc
     1741 gacgctcttg ccgctgcccg gcgcgccgca gcagatcgcc gggccaaagc cgctcgcggg
     1801 caagcatgtg ctgatcactt ccggaccgac gcacgagccg atcgatccgg tgcgctacat
     1861 cgccaatcgt tcgtcgggaa aacagggcca tgccatcgcc gcggcggctg tcgcggccgg
     1921 cgcgcgcgtg acgctggtgt cgggaccggt caccgtggcc gatccgagcg gggcgagcgt
     1981 ggtcagggtc gaaagtgccc gcgacatgct ggcggccgtc gagaaggcgc tgcccgccga
     2041 catcgccatc ttcgccgccg ccgtggcgga ctggcgcgtc gatcacgtgg cgggcgagaa
     2101 gatcaagaag ggcggctccg gcccgccgcg gctcgcgctg gtcgagaatc ccgacattct
     2161 ggcgacggtg tgccgcagca cgaggcggcc ggcactggtg gtcggcttcg ccgcggagac
     2221 ggaaaaggtg atcgagcacg ccagggccaa acgcgcccgc aagggctgcg atctcatcgt
     2281 cgccaacgac gtaagcccgg aaaccggcat catgggcggt gatcgcaaca cggtgcatct
     2341 cgtcggtcca gacagcgtcg aaacctggcc ggacctgccg aaggatgaag tcgcgcgccg
     2401 gcttgtcgca gcccttgccg agcgggcacc atgagccttg tgctgccgct gaagatcctg
     2461 ccgcacggcg cgggcctacc gctgccggcc catgccacgc cgggcgcggc ggggatggat
     2521 gtctgcgccg ctgtcgatac gccggtaacg ctggagccgg gggcacgggc gctgatcccc
     2581 tgcggcatcg ccatcgccgt gccggaaggc catgaggcgc agttgcgccc gcgttcgggc
     2641 cttgccctca agcatggcgt caccgtgctc aatgcgccag gcaccatcga ttccgactat
     2701 cgcggcgagg tgaaagcgct gctgatcaat cacgggcacg agccgttcgt gatcgagcgc
     2761 ggcatgcgga tagcccagat cgtcatcgcg gcggttgcgc gggtccgggt cacggtcgtc
     2821 ccggcgctgg acgacaccgc gcgcggcgcc ggcggctatg gttccaccgg gggcatcggc
     2881 cagagctgag gcgatcctgt cgccaaggct cgttgtgata gagatttctg ctttcttggc
     2941 ggcaaaaata acaagccgga agcaattgtt ttctcgattg caactactta ggacttgagc
     3001 cagcactctg aaggatgtcc accatgacca atgccgagcg ccgccccgga cgcggccgcg
     3061 tctattcttc catcaccgag accatcggcg acacgccgct ggtgcggctc gaccgtctga
     3121 cgaaggcgaa gggcattgag gcgaccctcg tcgccaagct ggagttcttc aatccgatct
     3181 cgagcgtaaa ggatcgtatc ggcgtcgcga tgatcgaggc gatggagcgc gacggccgca
     3241 ttgcgccggg ccggaccacg ctgatcgagc cgacgtccgg caatacgggc atcgcacttg
     3301 cctttgtcgc tgcggcgcgc ggctaccggc tgatcctgac catgccggaa tcgatgtcga
     3361 tcgagcggcg caagatgctg acctacctcg gcgcggagct tgtgctttca gaagccggca
     3421 agggcatgaa gggtgccatc gccaaggcgg aagagttggc ggcgacaatc cccggcgcga
     3481 tcattcccca gcagttccag aacccggcaa atcccgaaat ccaccggcgc acgacggccg
     3541 aggaaatctg gaacgatacg aatggcgcgg tcgatgtctt catcgccggc gtcggcacgg
     3601 gtggaaccat cacgggcgtc gggcaggtgc tgaaggcgcg caagcccgct ctgcgcgtgg
     3661 tggcagtcga gccggcggaa agccccgtgc tatccggcgg cgcgccggga tcgcacagga
     3721 ttcagggcat cggcgccggc ttcgtgccgg aaatcctcga cgtttccgta atcgatgagg
     3781 tggtgacgat cgacagccag gccgccatcg acacggcacg gctgctggcg cgcaccgagg
     3841 gcgtgccggg cggcatttcc gcaggcgcgg cgacggcagc ggcgctccag gttgcggcgc
     3901 ggccggacat gaagggcaag acgatcgtat tcatcgttcc ctcgttcgcc gagcgctata
     3961 tttccagcct cctgttcgag ggcctgtgag gcgcctgtcg gtcgtttctg atcaaatgcc
     4021 gaccggtttc gatgacctcg aactggagag catcgtcgct ggtcatacca tgctgtccaa
     4081 gctgaagcgc aactggcccg cggcctttcc gccgcatgct tcgcttctgg tcgcggtcga
     4141 agacggcccc atgctcctgg gcatcggagg tatcgcgccc gatccctatg acgtcgaacc
     4201 cggccgaggc cgcatccgcc atctctatgt tgcgcatgcc gcccggaggc tcggcgtcgg
     4261 gaggcgcatc ctgaattcct gcctcgactt tgcccggagc cggtttcaca ctgtctccct
     4321 gcgaacgccc gccagcgaac aggcgaaccg cttctacgag gcgcatggct ttcggccctt
     4381 gcacggccac gcaaacgcca gtcacgttct ttacctgccc cggcagccat agatccgggc
     4441 aggccaatcg gccattgccg cgccagggcg gatcggctat caccctgccc tgaagcgccc
     4501 gtagctcagc tggatagagc gttgccctcc gaaggcaaag gtcacacgtt cgaatcgtgt
     4561 cgggcgcgcc a
//
LOCUS       DAOMXN010000203         4566 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_10129_length_4566_cov_13.507426, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000203 DAOMXN010000000
VERSION     DAOMXN010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10129_length_4566_cov_13.507426"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..1102)
                     /locus_tag="PK812_11900"
     CDS             complement(56..1102)
                     /locus_tag="PK812_11900"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HRJ70296.1"
                     /translation="MAGAHDLKDRIEERVNTGLLGQWYPVAKSSEVQPGKPFGVKALG
                     QHIVLWRDKAGKIQCVEDYCPHRGARMSRGEVHNGHIACRYHGVVVDGSGTIVSVPAM
                     PDCPLEGRKAVQAYAVTELYDAIFAYFPSVEAPTPPPLTMPEEFNDPEWTGFLCASPW
                     ACNYRYALDNLADPMHGCFLHADSFTLAYGSKQDLMKLDKAEDGFIVSRVNQQGKNFD
                     YTHMVFGSATYCRLDIPYPKAAGPGSFMRILGYCLPVDENNCRVFFWRMRQVTGFERD
                     SWRFLYRAKLEPRHWKVLEQDREMLSAMPDDARKREMLYQHDIGVSRIRQILTRMAKS
                     QIAAEDAAAARSAG"
     gene            complement(1146..1907)
                     /locus_tag="PK812_11905"
     CDS             complement(1146..1907)
                     /locus_tag="PK812_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008597323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ70297.1"
                     /translation="MSLKDLAAILAVALVGLVAWEFLPPAFGVPRYIIPTFSQTWTEL
                     LRMAKQDNLARHFISTATMTALGFALGSALGATMGYLLGLSRFWERILSPYILGLQIA
                     PKVAFAPLFIMWFGYTIWPKLLVALLIVFFPILVNVLQAMKTVDRDMVNLARAYSMNR
                     WQIFWKIEFPASLPALMAGLRIGATLAVIGVTVGELVGGNTGLGFLITYGEGQANAAM
                     VFNAIMLLTVIGIVLYMLVAAAEERLLHYLPKVQK"
     gene            complement(1976..2953)
                     /locus_tag="PK812_11910"
     CDS             complement(1976..2953)
                     /locus_tag="PK812_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008597326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRJ70298.1"
                     /translation="MFRSWKTPLMAAALCTVAFAAQAQQKLVYLFPAADFLPAFAPFH
                     IAKAKGYYKQAGLDVEFQVGKGGADVAKQVAVGNADMGGGIGDTPIIVRANGLTVKGV
                     ALLGGRTLTAITARKDANVKSFADLKGKKIGVLAFQDTTYYNLLGVLKSANLGKDDAN
                     IQAVGPAGVVQLMISGDLQAISGVPEWTAAIEAAGIAVDVFDIESVFPAMAQAILASD
                     KIIAERPAAVKGFVNATLKAIREIMADPEAASKAYVAAVPQHAGKEKAMEAIMRRYVT
                     AVYAHKTPADLGKFDAERMAKVQKFYVDNAIVQTAVPIADTFTNAMIGN"
     gene            complement(2986..3771)
                     /locus_tag="PK812_11915"
     CDS             complement(2986..3771)
                     /locus_tag="PK812_11915"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ70299.1"
                     /translation="MQPAVAALEFAGVTRAFAQKSGTPLVAVRDVSFVVKPGEFVSVV
                     GPSGCGKSTILNMTAGLDEPTEGVVRVSGEIVKGPNSHVGFMLQKDLLLPWRSIVRNV
                     EFGLETRGLTRREMRERALLELAHCKLAEFAEHYPHQLSGGMRQRAALARTLAIRPDI
                     ILLDEPFSALDAQTKMLLQRNFGATIAETGVTALLITHDLAEAVAMSDRVLVMSERPG
                     HIIEEIVIDLPNRENPMARRAHPRTGGFIARMLELLHLNDRAA"
     gene            complement(3746..4078)
                     /locus_tag="PK812_11920"
     CDS             complement(3746..4078)
                     /locus_tag="PK812_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70300.1"
                     /translation="MSEMHASLGTHRGDAARKAASSGSDVHAALTRQSLGRPLSELEE
                     ALADAMEDIYASGFHDMSALAEHLTQRGVRAPRSRETVWTIDLLKTELAAINASLDEA
                     YAAGGRGA"
     gene            4307..>4566
                     /locus_tag="PK812_11925"
     CDS             4307..>4566
                     /locus_tag="PK812_11925"
                     /inference="COORDINATES: protein motif:HMM:NF013482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HRJ70301.1"
                     /translation="MSRIEDARLVTGQGCFVGDLLPEGCLVLEFVRSQAASGRLKSVE
                     AGEARALRGVAAVLTADDLPSREPLAINPLVPGIAAPPAALLA"
BASE COUNT          778 a         1431 c         1543 g          814 t
ORIGIN      
        1 tgccgcgcgc cgcgccggtg atgacgatgg tacgtccctc cagcatgcgc ggtcttcagc
       61 cggccgagcg ggcggcggct gcatcctcgg ccgcgatctg gcttttcgcc atgcgggtga
      121 gaatctgccg gatgcgcgac acgccgatgt catgctggta gagcatctcg cgcttgcggg
      181 catcatccgg catggcggac agcatctcgc ggtcctgctc cagcaccttc cagtggcgcg
      241 gctcaagctt ggcgcggtag agaaaccgcc agctgtcgcg ctcgaagccc gtcacctgcc
      301 gcatccgcca gaagaagacg cggcagttgt tctcgtccac cggcaggcaa tagccgagaa
      361 tgcgcatgaa gctgccgggg ccggccgcct tcggataggg aatgtccagc cggcaatagg
      421 tggccgatcc gaacaccatg tgggtgtagt cgaagttctt gccctgctgg ttgacgcggg
      481 agacgatgaa gccgtcttcg gccttgtcca gcttcatcag atcctgcttg gagccgtagg
      541 ccagggtgaa gctgtcagca tgcaggaagc agccgtgcat cgggtcagcc agattgtcga
      601 gcgcatagcg gtagttgcag gcccaggggg aggcgcagag aaagccggtc cattccggat
      661 cgttgaattc ctccggcatg gtgagcgggg gcggggtcgg cgcctccacc gaagggaaat
      721 aggcgaaaat cgcgtcgtaa agctcggtca ctgcgtaggc ctgcaccgcc ttgcggccct
      781 ccagcgggca atccggcatg gccggcacgc tgacgatcgt gccggaccca tcgacgacca
      841 cgccgtgata gcggcaggcg atgtggccgt tgtgcacctc gccgcgcgac atgcgggcgc
      901 cgcgatgcgg gcaataatcc tccacgcact ggatcttgcc cgccttgtcg cgccacagca
      961 cgatgtgctg gccgagcgcc ttgacgccga aaggcttgcc cggctgcacc tcggaggatt
     1021 tggccacggg gtaccactga ccgagcaggc cggtgttgac ccgctcctcg atgcgatcct
     1081 tcagatcgtg tgcgccggcc atgacgtcct cccggaattg ccgtgggttg cgtgtgtgcc
     1141 gccggtcatt tctggacctt gggcagatag tgaagaagcc gctcctcggc tgccgccacc
     1201 agcatgtaga gaacgatgcc gatcaccgtc aggagcatga tggcgttgaa caccatggcg
     1261 gcgttggcct ggccctcgcc gtaggtaatg aggaacccca gtccggtatt gccgccgacg
     1321 agttcgccga ccgtgacgcc gatcacggcg agggtcgcgc cgatgcgcag gcccgccatc
     1381 agcgcgggca gggaggctgg aaactcgatc ttccagaaaa tctgccagcg gttcatggaa
     1441 taggcgcgcg ccagattgac catgtcgcgg tccacggtct tcatcgcctg caacacgttg
     1501 acgaggatcg ggaagaacac gatcagcagc gcgaccagca acttcggcca gatcgtgtag
     1561 ccgaaccaca tgatgaacag cggcgcgaaa gccactttcg gggcaatctg caagcccagg
     1621 atgtaggggg agaggatgcg ttcccagaag cgcgacaggc cgagcaggta gcccatggtc
     1681 gcgccaaggg cgctgcccag cgcgaagccg agggcggtca tcgtcgcggt cgatatgaag
     1741 tggcgcgcga gattgtcctg tttcgccatg cgcagcaatt ccgtccacgt ctgcgagaac
     1801 gtcggaatga tgtagcgtgg cacgccgaac gccggcggca ggaactccca ggcgacaagg
     1861 cctacgagcg ccacggcgag aatggcggcg aggtctttca gggacatgag ggcttgcctg
     1921 acgggatggc ggggcgtcgc ccggctgcgg tcagccgggc ggcgcggtta gcgtctcagt
     1981 tgccgatcat ggcgttggtg aaggtgtcgg cgatcggcac ggcggtctgc acgatggcgt
     2041 tgtcgacgta gaacttctgc accttcgcca tgcgctcggc gtcgaacttg ccgaggtcgg
     2101 ccggggtctt gtgtgcatag acggccgtca catagcgacg catgatcgct tccatcgcct
     2161 tttccttgcc cgcgtgctgg ggcacagcgg cgacataggc cttcgacgcg gcttccggat
     2221 cggccatgat ttcgcggatc gccttcagcg tggcgttgac gaaaccctta accgcagccg
     2281 ggcgctcggc gatgatcttg tccgaggcga gaatggcctg ggccatggcc ggaaagacgc
     2341 tctcgatgtc gaagacgtcc acggcaatgc cggccgcctc gatggcggcg gtccactccg
     2401 gcacgccgga aatcgcctgg aggtctcccg agatcatgag ctggacgacg ccagctggtc
     2461 caaccgcctg gatgttggcg tcgtccttgc caaggttcgc gctcttcagc acgccgagga
     2521 ggttgtagta ggtggtgtcc tggaaggcga ggacaccgat cttcttgccc ttgaggtcgg
     2581 cgaagctctt cacattcgcg tccttgcggg cggtgatcgc ggtgagcgtg cggccgccga
     2641 ggagcgcgac gcccttcacc gtgaggccgt tggcgcgcac gatgatcggg gtgtccccga
     2701 tgccgccgcc catgtcggcg ttgccgacgg cgacctgctt ggccacgtcc gcaccgccct
     2761 tgccgacctg gaactccacg tcgaggcccg cctgcttgta atagcccttg gccttggcga
     2821 tgtgaaacgg cgcgaaagcc ggcaggaagt cggcggccgg aaacagatag accagttttt
     2881 gctgggcctg cgcggcaaag gccacggtgc acagcgccgc cgccatcagc ggcgtcttcc
     2941 aggacctgaa catgaaatcc tccctctctt ggactggacg cgccgtcagg ccgcccgatc
     3001 gttcagatgc aaaagttcga gcatgcgggc gatgaaaccg ccggtgcgcg ggtgcgcccg
     3061 gcgggccatc ggattctcgc ggttgggcag atcgatgacg atttcctcga tgatgtggcc
     3121 gggacgttcg ctcatcacca gcacgcgatc ggacatcgcc accgcctccg ccagatcatg
     3181 ggtgatcagc agtgcggtga cgccggtctc ggcaatcgtc gcgccgaaat tccgctggag
     3241 cagcatcttg gtctgggcgt cgagcgcgga gaacggctcg tccagcagga tgatgtcggg
     3301 ccggatcgcc agcgtgcggg cgagcgcggc gcgctggcgc atgccgccgg acaactgatg
     3361 gggatagtgc tcggcgaatt cggcaagctt gcagtgcgcc agctcaagca gcgcgcgttc
     3421 acgcatttcg cgccttgtca gaccccgcgt ttccaggccg aactcgacat tgcgcacgat
     3481 cgagcgccac ggcagcagga ggtccttttg cagcatgaag ccgacatggc tgttcggccc
     3541 cttcacgatc tcaccgctga cgcgcacgac gccttcggtc ggctcgtcca ggccggcggt
     3601 catgttgagg atcgtgctct tgccgcatcc cgacggacca acgaccgaga cgaactcacc
     3661 cggcttcacc acgaaactca catcgcgcac cgcgaccagc ggcgtgccgg atttctgggc
     3721 gaaggcgcgg gtgacgcccg cgaattcaag cgccgcgacc gccggctgca taggcttcat
     3781 ccagcgaggc gttgatggcg gcgagttcgg tcttcagcag gtcgatggtc cagacggtct
     3841 cacgcgacct tggcgcacgc acgccacgct gcgtcaggtg ctccgcgagc gccgacatgt
     3901 cgtgaaaacc gctggcgtag atgtcctcca tcgcatcggc gagcgcttct tccagttcgc
     3961 tcagaggacg ccccagggac tgacgggtga gcgccgcatg gacatcggag ccggagcttg
     4021 cggcctttct cgcggcatcg ccccgatgcg tgcccagcga agcatgcatt tcgctcatcc
     4081 cggcctccca tcatcgtcga ttttgttcaa atatgaacat actgttcatt atttaatagc
     4141 aaggggtgcg cctcgggtca agccgattgt ccggctcgtc cggcttgcca gagccctggc
     4201 gaggggtcag aaagcgggaa gctgaatccc ccgtgcgggt cgtatcatga tgacaggaag
     4261 cgtttccgat ccagcccggt ctggtggcga tggaatcggt gcggcggtgt cgcgcattga
     4321 ggacgcgcgc ctggtgaccg gtcagggatg cttcgtgggc gacctgctgc ccgaaggctg
     4381 cctggtcctg gaattcgtgc gcagccaggc tgcgtccggg cggctgaagt cggtggaagc
     4441 gggtgaggcg cgggcgctgc ggggtgtcgc cgccgtgctg acggccgacg atctgccctc
     4501 gcgggagccg ctggcgatca atcctcttgt cccggggatc gccgcgccgc ctgcggcgtt
     4561 gctggc
//
LOCUS       DAOMXN010000204         4565 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_10130_length_4565_cov_10.315299, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000204 DAOMXN010000000
VERSION     DAOMXN010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4565
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10130_length_4565_cov_10.315299"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="PK812_11930"
     CDS             complement(<1..421)
                     /locus_tag="PK812_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HRJ70302.1"
                     /translation="MSDRHEPMTPAEHAARRAAVTAALRAETGIDEAMIERLVRAFYH
                     RVQADPLLGPVFAARIADWEPHLERMFAFWSSVTLLTGVYHGRPMERHMALDVDAAHF
                     DRWLALFEETAAACCPAKAAAHFVARARAIAASIEQGL"
     gene            718..1335
                     /gene="rpsD"
                     /locus_tag="PK812_11935"
     CDS             718..1335
                     /gene="rpsD"
                     /locus_tag="PK812_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018044769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HRJ70303.1"
                     /translation="MTKRVDAKHKIDRRMGENIWGRPKSPLNRREYGPGQHGQRRKGK
                     LSDFGTQLKAKQKLKGYYASISERQFRSYYAEAIRMKGDAGENLIGLLERRLDAVVYR
                     AKFVPTVFAARQFVNHGHVKVNGRRVNIGSYLVKPGDVVEVKEASREMLMVLESKQLG
                     ERDVPDYVEVDHNKMTAKLSRVPTLGEVPYPVKMEPNLVVEFYSR"
     gene            1460..1663
                     /locus_tag="PK812_11940"
     CDS             1460..1663
                     /locus_tag="PK812_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70304.1"
                     /translation="MWKVATLVHIMAMTVLMGVLVLVILSVPSLTEQGMRLIPVAALI
                     GFVVGIPFCVVAAKAILARTAGR"
     gene            1750..3111
                     /locus_tag="PK812_11945"
     CDS             1750..3111
                     /locus_tag="PK812_11945"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tad domain-containing protein"
                     /protein_id="HRJ70305.1"
                     /translation="MGVLNRFLDCGFDLRRSEQGAVAPIFAVSALAVFVILGASLDYN
                     RIEKARAQLQSSTDATAISLAIQRANGLKDEQLSDAATKRLRASVPPDLQEGIRSVGA
                     EGLGSKVSVRSLAAVPLGFAGILGKSEVVVEAQSKADFGSAGATNKVEVALVVGLTGG
                     VNEKGRLEALKRQTQAFTDKAREAAEGKDTIKLALVPYAEHVKLKPVYRPAPWLSFAG
                     NEEKPTLTGEQQSKASSERMAAFKLALKSHQLKIERMAWTGCVTDTKDPTIIAFDAAN
                     QHPGVICASNEWHSAQGNVIEPLTARHDQIRQQAGQMVAEGCENTTLGLVWGMGALLP
                     QGPFGTAAPANDAETRKVLILVSSAPNQVNRLNGICIAPGQREPKLNHATATVCDRIK
                     RQGVTIYAVNLIDGDPEVLKACASDLTKYHHANSAGDLQRVMDEVAKEVLVQAPKMAR
                     LVN"
     gene            complement(3121..3678)
                     /locus_tag="PK812_11950"
     CDS             complement(3121..3678)
                     /locus_tag="PK812_11950"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRJ70306.1"
                     /translation="MGKRAIDVDILMVPGWKNSGPEHWQSRWEQRLSTARRVEQPDWM
                     RPNRDIWVGNILKAIDAARQPVVLVAHSLGVIACLHALPKAKKGDVIGAFLVAPIGET
                     VIETEEAIDHDFLPIPRKKLEIPSVVVASHDDPYAPFLTSEELAYGWGAELLDAGHAG
                     HINTASGYGPWPEGLMKFAGFLAKL"
     gene            complement(3780..>4565)
                     /locus_tag="PK812_11955"
     CDS             complement(3780..>4565)
                     /locus_tag="PK812_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HRJ70307.1"
                     /translation="RAEVATCAISGAVGTFANIDPRVEEHVATMLGLAVEPVSTQVIP
                     RDRHAMFFATLGVIASSIERLAIEIRHLQRSEVYEAEEFFSEGQKGSSAMPHKRNPVL
                     TENLTGLARMVRAYALPAMENVALWHERDISHSSVERMIGPDATVTLDFALHRLTGVV
                     DKLVVYPENMQKNLDRLGGLVHSQRVLLALTQKGVSREDSYRLVQRNAMPVWRGEGNF
                     LDLLKKDAEVKARLSDSELEALFDLAYHYKHVDTIFARVFGKV"
BASE COUNT          812 a         1433 c         1563 g          757 t
ORIGIN      
        1 ccagcccctg ttcgatgctt gcggcgatgg cccgtgcccg cgccacgaaa tgcgcggcag
       61 ccttcgcggg gcaacatgcg gccgccgtct cctcgaacag ggcaagccag cgatcgaaat
      121 gcgcggcgtc gacgtcgagc gccatgtgcc tttccatcgg ccggccgtga tagaccccgg
      181 tcagcagcgt caccgacgac cagaaggcga acatccgctc cagatgcggc tcccagtcgg
      241 cgatccgcgc ggcgaagacg gggccgagca gcggatcggc ctgcacccga tgataaaacg
      301 cgcgcaccag gcgctcgatc atggcctcgt cgatgccggt ttcggcgcgc agggcagcgg
      361 tgaccgccgc gcggcgggcg gcgtgctcgg cgggcgtcat gggctcgtgg cggtcggaca
      421 tgcgggttga gatagcgtct cgggccacag agggaaatcc ccgcgttgtc gcaggtttga
      481 caagccgcat cgcttccact agagcaccgg caccttcagc gcgggccctg atcggcccgc
      541 gttgcagttt gtttgcgcgt ggtccgttcg aaaatccgca aggcactttt cgggatcgtg
      601 cgcgagacgc acccgtggcc gtcccgttcc ggtgattgcc tgtcggccgt gccctgcaaa
      661 ggaccggcag aggagggcgc gttcctcata acctgttgaa tagaaagagg aaccgcgatg
      721 acaaagcgcg ttgatgccaa gcacaaaatc gatcgccgca tgggcgagaa catctggggc
      781 cgtccgaaaa gcccgctgaa ccggcgcgag tacggcccgg gccagcacgg ccagcgccgc
      841 aagggcaagc tctcggactt cggcacccag ctcaaggcca agcagaagct gaagggctac
      901 tacgcctcga tctccgagcg ccagttccgc agctactatg cggaagccat ccgcatgaag
      961 ggcgacgccg gcgagaacct gatcggcctg ctggagcgcc gcctcgatgc ggtcgtctat
     1021 cgcgccaagt tcgtgccgac ggtgttcgcc gcccgccagt tcgtcaacca cggtcacgtg
     1081 aaggtcaacg gacgccgtgt caacatcggc tcctacctgg tgaagccggg cgatgtggtt
     1141 gaagtgaagg aagcctcgcg cgagatgctg atggtgcttg agtccaagca gctcggcgag
     1201 cgcgatgtgc cggactacgt cgaagtcgac cacaacaaga tgacggcaaa gctcagccgc
     1261 gtcccgacgc tcggcgaggt gccctatccg gtgaagatgg agccgaacct ggtggtcgag
     1321 ttctactcgc gctgacgccg gctccgcatc ctgcgacatt tgggccgcct tcgggcggcc
     1381 tttttgttgc gctgtgtcaa cgaccggcat ggacgccggc gcaggatgac gcgtgtcata
     1441 tccgcgaaaa ggtgacttca tgtggaaggt tgcgaccctg gttcacatca tggccatgac
     1501 ggtgctcatg ggcgtgctcg ttctggtgat cctcagcgtg ccgtcgctga ccgagcaggg
     1561 catgcggctg attccggtgg cggcgctcat cggtttcgtc gtgggcatcc ccttctgcgt
     1621 cgtggcggcc aaggcgattc tggcgcggac agccggccga taggctgaaa gccgggcctt
     1681 tccggcgcaa ggccgttaaa ctttcctggg tagacttgcg gctctccgat gttgcgaggg
     1741 tcgcagggca tgggtgtcct caaccgattt ctcgattgcg gctttgacct ccgccgatcc
     1801 gagcagggtg ccgtcgcacc gatcttcgcc gtttccgcgt tggcggtctt cgtcatcctg
     1861 ggcgcctcgc tcgattacaa ccgcatcgag aaggcgcggg cacagcttca gtcctcgacg
     1921 gacgccacgg cgatctcgct ggcgatccag cgcgccaacg ggctgaagga cgagcagttg
     1981 tccgatgccg cgaccaagcg cctgcgcgcc tcggtgccac cggacctgca ggaggggatc
     2041 cgctcggtgg gcgcggaggg actgggctcc aaagtctcgg tccgttcgct ggcggcggtg
     2101 ccactcggct ttgccggaat cctgggaaaa agcgaggtcg tcgtcgaggc ccagtcgaag
     2161 gcggatttcg gttcggccgg tgccacgaac aaggtcgagg tggcgctggt ggtcgggctg
     2221 accggcgggg tcaacgaaaa gggccggttg gaagcgctga agcggcagac gcaggccttc
     2281 acggacaagg cgcgcgaggc cgccgaaggc aaggacacga tcaagctggc gctggttccc
     2341 tatgccgaac acgtgaaact gaagccggtc tatcgcccgg cgccgtggct gagcttcgcc
     2401 ggcaacgagg agaagccgac gctgacaggt gagcagcagt ccaaggcttc gagcgagcgc
     2461 atggcagcct tcaagctggc gctcaagtca catcagctca agatcgagcg catggcctgg
     2521 accggctgcg tcaccgacac caaggacccg acgatcatcg ccttcgacgc ggccaaccag
     2581 catcccggcg tgatctgcgc cagcaacgag tggcattccg cgcagggcaa cgtcatcgaa
     2641 ccactgacgg cgcgccatga ccagatcagg caacaggccg gccagatggt ggcggagggc
     2701 tgcgagaaca ccacgctcgg gctggtctgg ggcatgggcg cgctgctgcc gcaggggccg
     2761 ttcggcacgg cggcgccggc gaacgacgcg gaaacacgca aggtgctgat cctggtctca
     2821 tccgcaccca atcaggtgaa caggctgaac ggcatctgca tcgcgcccgg ccagcgggag
     2881 cccaagctga accatgcgac ggcgacggtc tgcgaccgga tcaaacggca aggcgtcacg
     2941 atctatgcgg tgaacctgat cgatggcgat ccggaggtgc tgaaggcctg cgcgagcgac
     3001 ctgacgaagt accatcacgc caattccgcc ggcgacctgc agcgggtgat ggacgaggtg
     3061 gcgaaggaag tgctggtcca ggcgccgaaa atggcgcggc tggtgaactg aacccgcgcg
     3121 tcagagcttc gctagaaagc cggcgaattt catcagccct tccggccagg ggccgtagcc
     3181 cgaggcggtg ttgatgtgcc cggcatggcc ggcgtccagg agttcggctc cccagccgta
     3241 ggccagctct tccgaggtga ggaaaggcgc gtaagggtcg tcgtgactgg cgacgacgac
     3301 ggagggaatc tccagcttct tgcgcggaat cggcaggaag tcgtggtcga tcgcctcctc
     3361 cgtctcgatc acggtctcgc cgatcggggc gaccaggaac gcgccgatga catcgccctt
     3421 cttcgccttc ggcagggcat gcaggcaggc gatcacgcca aggctgtggg cgaccagcac
     3481 gaccggctgg cgggcggcat caatggcctt caggatgttg ccgacccaga tgtcgcggtt
     3541 cggccgcatc cagtccggct gctcgacgcg ccgcgccgtg gacaggcgct gctcccagcg
     3601 tgactgccag tgctccgggc cggaattctt ccagcccggc accatgagaa tgtcgacatc
     3661 aatggcgcgc ttgcccaacg gcttgctcct ctagcggcgg cagattgccc cgatccatgg
     3721 catcagggca agagaaagcg ccgcgcgcct tgatccatgt ccatgcgcga gggtggctct
     3781 cagaccttgc cgaagacccg tgcgaagatc gtgtcgacgt gcttgtagtg ataggcgagg
     3841 tcgaacagcg cctcgagttc gctgtcggac agtcttgcct taacttcggc gtccttcttc
     3901 agaaggtcga ggaagttgcc ctcgccgcgc cagaccggca tcgcgttacg ctggacgagg
     3961 cggtagctgt cctcgcggct gacgcccttc tgggtgaggg cgagcagcac ccgctgcgaa
     4021 tgcacaaggc cgccgagccg gtccaggttc ttctgcatgt tctccggata gaccaccagc
     4081 ttgtcgacga caccggtgag gcggtgcagc gcgaaatcga gggtcaccgt cgcgtcgggg
     4141 ccgatcatgc gctccaccga ggaatgcgag atgtcgcgct cgtgccagag cgccacgttt
     4201 tccatcgcgg gcagggcata ggcgcgcacc atgcgggcaa ggccggtgag gttttccgtc
     4261 agcaccgggt tgcgcttgtg cggcatggcg gaggagccct tctggccctc cgagaagaac
     4321 tcttccgcct catagacttc cgagcgctgg aggtgccgga tttcgatggc gagccgctcg
     4381 atggacgagg cgatgacgcc gagcgtcgcg aagaacatgg cgtggcggtc gcgcggtatg
     4441 acctgcgtcg agaccggctc gacggcgagg ccgagcatgg tcgccacgtg ctcctcgacg
     4501 cgcggatcga tgttggcgaa ggtgccgacg gcgccggaga tggcgcaggt ggccacttcg
     4561 gcgcg
//
LOCUS       DAOMXN010000205         4532 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_10245_length_4532_cov_9.420818, whole genome shotgun sequence.
ACCESSION   DAOMXN010000205 DAOMXN010000000
VERSION     DAOMXN010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4532
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10245_length_4532_cov_9.420818"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1222)
                     /gene="mltG"
                     /locus_tag="PK812_11960"
     CDS             complement(<1..1222)
                     /gene="mltG"
                     /locus_tag="PK812_11960"
                     /inference="COORDINATES: protein motif:HMM:NF014656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRJ70308.1"
                     /translation="MADRNAPGGPAGERKRSLFSPRDKLKPVAAPPPPAKPKKRGGGI
                     VGKLSAFLTVFVLLLVGATAGVSYFKSVVSAPGPLQTDRAVVIERGAGTDEIAELLER
                     EGVINRRVTFELLAYSHIYGKLKAGEFLFRREASLLEVIDTIANGRAIEHSITIPEGL
                     TSEQIVARIRDNELLVGTVADIPPEGAILPDTYKIARGTSRDALLARMIRDQRRILNE
                     VWAKRAKELPIRTPQDLVTLASIIEKETGKHDERSRVAGVFVNRLNRGMKLQSDPTII
                     YGLVGGKGSLGRPITTSDIQSNTAYNTYVIPGLPPGPIANPGRASLEAAANPSRTRDL
                     FFVADGSGGHAFAETLEQHNRNVARWRQIEAQQKEAAASPPVAAPGATGAPAFAPVAP
                     INPAVPPGTRGDAAP"
     gene            complement(1301..2563)
                     /gene="fabF"
                     /locus_tag="PK812_11965"
     CDS             complement(1301..2563)
                     /gene="fabF"
                     /locus_tag="PK812_11965"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HRJ70309.1"
                     /translation="MRRVVVTGMGMVTPHGCGVEPTWKRILAAESAARKIERFDVSDI
                     AAKIACMIPRGSGPGEFNPDDWMEPKEQRKVDDFIVFAMAASKQALDDANWHPKDYED
                     QIATGVLIGSGIGGINGISQATDILREKGPRRLSPFFIPGRLINLAGGYVSIEHGLKG
                     PNHAVVTACSTGAHAIGDAARIIALGDADVMVAGGTESPVERLPLAGFASCRALSTNF
                     NDEPHRASRPYDKDRDGFVMGEGAGVVVLEELEHARARGARIYAEVIGYGMSGDAYHI
                     TSPTPDGDGAYRCMRAALKRAGITPDEIDYVNAHGTSTPLGDEIELGAVARLVGNSAA
                     TLSMSSTKSAVGHLLGAAGAVEAIFTILAIRDQIAPPTLNLDNPSVETPIDLVPHKAK
                     PRKIDVALSNSFGFGGTNASLVFRRYAA"
     gene            complement(2598..2834)
                     /locus_tag="PK812_11970"
     CDS             complement(2598..2834)
                     /locus_tag="PK812_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018044468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRJ70310.1"
                     /translation="MSDIAERVKKIVIEHLSVEADKVVDKANFIDDLGADSLDTVELV
                     MAFEEEFGVEIPDDAAETIVTVGDAIRFLEKNAA"
     gene            complement(3068..3805)
                     /gene="fabG"
                     /locus_tag="PK812_11975"
     CDS             complement(3068..3805)
                     /gene="fabG"
                     /locus_tag="PK812_11975"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HRJ70311.1"
                     /translation="MFSLAGKNALVTGASGGIGAAIAEALHAQGAVVTLSGTRREALD
                     KLAERLGDRVHVATANLSDRASVEALLPAAETAMGQVDILVNNAGITRDNLAMRMKDE
                     EWDQVLAVNLSAGFVLARAALRGMMRRRFGRIIGITSVVGVTGNAGQANYAAAKAGMI
                     GMSKALAAEVASRGVTVNCVAPGFIETAMTDALNDKQKEAILGNVPAGRLGSVAEIAA
                     AVVFLASNEAAYVTGQTLHVNGGMARI"
     gene            complement(4058..>4532)
                     /locus_tag="PK812_11980"
     CDS             complement(4058..>4532)
                     /locus_tag="PK812_11980"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HRJ70312.1"
                     /translation="QGEVCAVANDNGGGQVVISGTKAAIDRAIALAPSKGIRRAMPLP
                     VSAPFHCALMQPAAEAMAEALAKATINVPNVPVVANVLASAITDPAEIRARLVEQVTG
                     SVRWAESMAWLGTKGVTRFVEIGAGKVLSGLVKRIVEGATGANVGTPEDVAGYQG"
BASE COUNT          744 a         1540 c         1451 g          797 t
ORIGIN      
        1 gaggcgcagc gtcgccccgt gttcccggtg ggaccgccgg attgatgggc gcgaccggcg
       61 cgaaggcagg cgccccggtt gccccgggag cagcgacagg cggagaggca gccgcctcct
      121 tctgctgcgc ctcgatctgc cgccagcgcg cgacattgcg gttatgctgc tccagcgtct
      181 cggcaaaggc gtggccaccc gacccatcgg ccacgaagaa aagatcgcgg gtccgcgagg
      241 ggttggctgc cgcctcaagc gaggcgcgcc ccggattggc gatcgggccg ggcggcagcc
      301 ccggaatgac ataggtgttg taggcggtat tggactggat gtctgacgtc gtgatcggtc
      361 ggccaagcga gccctttccg cctaccagac cgtagatgat cgtgggatcg gattgcagct
      421 tcatgccccg gttgagacga ttgacgaaca cgccggccac gcgcgagcgc tcatcgtgct
      481 tgcccgtttc cttctcgatg atcgaggcga gcgtgaccag gtcctgcggc gtgcggatcg
      541 gcaactcttt cgcgcgcttg gcccagacct cgttcaaaat ccgcctctga tctcggatca
      601 tcctggccag cagcgcatcg cgggacgtgc cgcgcgcgat cttgtaggta tccggcagaa
      661 tggcgccttc cggcgggata tcggccacgg ttccgaccag cagttcgttg tcgcgaatgc
      721 gcgccacgat ctgttcgctg gtcaggcctt cgggaatggt gatcgaatgc tcgatcgccc
      781 ggccattcgc gatggtatcg atgacttcga gcaagctcgc ttcgcgccgg aacaggaact
      841 ctccggcctt cagcttgccg tagatgtgcg aataggccaa caactcaaac gtcacgcgac
      901 ggttgatcac gccctcgcgt tccagcagtt cggcgatctc gtcggtgccg gcgccgcgct
      961 cgatgaccac ggcgcgatcc gtctgcaagg gcccaggagc agacaccacg gacttgaaat
     1021 agctgacgcc ggcggtagcg ccgaccagga gcaggacgaa gacggtcagg aacgccgaca
     1081 gcttgcccac gataccgccg ccccgcttct tcggcttggc cggcggcgga ggagccgcga
     1141 cgggcttcag cttgtcgcgc gggctgaaca gagagcgctt gcgctcgccc gcggggccgc
     1201 ctggggcgtt cctgtcagcc attcacaggt ctccggatct tcatgccact atcatgccga
     1261 aaaatgtggc gaaagggaga ggcccgatcg ccgcgcatcc tcaggctgcg taccgccgaa
     1321 acaccagcga cgcattggtg ccgccaaatc cgaaggagtt cgacagggcc acatcgatct
     1381 ttcgcggctt ggccttgtgc gggacaagat cgatcggcgt ctcaaccgac ggattgtcca
     1441 ggttgagcgt tggcggcgcg atctggtcgc ggatcgccag gatggtgaag atcgcctcca
     1501 ccgcgccggc tgccccgagc aggtgaccga cggccgactt ggtcgacgac atggacaggg
     1561 tggctgccga attgccgacc agacgggcaa ccgcgccgag ttcgatctcg tcgccgagcg
     1621 gcgtcgaagt cccgtgcgcg ttcacgtagt cgatctcatc cggtgtgatg cccgcccgct
     1681 tcagcgcggc ccgcatgcag cgataggcgc catcgccatc cggcgtcggc gaggtgatgt
     1741 gataggcatc gcccgacatg ccgtatccga tgacctcggc atagatcctg gcgccgcgag
     1801 cccgggcatg ctcaagctct tccagcacga ccacgccggc gccttccccc atcacgaacc
     1861 cgtcacggtc cttgtcgtaa gggcgcgagg cccggtgcgg ctcgtcgttg aagttggtcg
     1921 acagcgcgcg gcaggacgcg aacccggcca gcggcaggcg ctcgaccggc gattccgtgc
     1981 cgcccgccac catcacgtcg gcatcgccaa gcgcgatgat gcgggctgcg tcgccgatgg
     2041 cgtgcgcgcc cgtggagcag gccgtcacca ccgcatggtt cgggcctttc agcccgtgct
     2101 cgatggaaac ataaccgccg gccaggttga tcagccgtcc cgggatgaag aacggcgaga
     2161 gccggcgcgg gcctttttcc cgaaggatgt cggtggcctg gctgatgccg ttgatgccgc
     2221 cgatccccga gccgatcagc acgcccgtcg cgatctggtc ctcatagtcc ttgggatgcc
     2281 agttcgcgtc gtccagcgcc tgtttggatg ccgccatcgc gaagacgatg aaatcatcga
     2341 ccttgcgctg ttccttcggc tccatccagt cgtccgggtt gaactctccc ggaccggaac
     2401 cgcgcgggat catgcaggcg atcttggccg cgatgtcgga cacatcgaaa cgctcgatct
     2461 tccgcgcggc gctttcagcc gcgagaatgc gcttccaggt gggctcgacg ccgcagccgt
     2521 gcggcgtcac catgcccatc ccggtcacga cgacccggcg caatcccgac atgccctgct
     2581 cccggccctg gcgcggatca ggccgcgttc ttctcaagga aacggatggc gtcgccgacc
     2641 gtcacgatgg tctcggcggc atcgtccggg atttccacgc cgaactcttc ttcgaaagcc
     2701 atcaccagct cgaccgtgtc gagcgagtcc gcgcccagat catcgatgaa gttggccttg
     2761 tccacgacct tgtcggcttc gacggacagg tgctcgatga cgatcttctt cacgcgttcg
     2821 gcgatgtcgc tcatgatgcg tatccttgaa tagtttgtac tgccccgttc accacggctt
     2881 taggccgtga gaacgaccgc cggctgaaac tcgtaaaacg acgagcgccg cagagcaata
     2941 acccgcgccg tccgaaccgc ttcgcctctc cattgttgga accgcagcga aagtcaaccg
     3001 cccggcccgc tgatgccaca tcgggcgtgg cagttttttg acggagcgcc gcccaggaac
     3061 cggttgctca gatgcgcgcc atgccgccgt tgacgtgcag cgtctggccg gtgacatagg
     3121 ccgcctcgtt ggaggccaga aagaccaccg ccgccgcgat ttccgccacc gatccaagcc
     3181 gcccggctgg cacgttgccg agaatggctt ccttctgctt gtcgttcagc gcatcggtca
     3241 tcgccgtctc gatgaagccc ggcgcgacgc agttcaccgt gacgccgcgg ctggcgactt
     3301 ccgccgcgag cgccttcgac atgccgatca tgccggcctt cgccgcagcg taattcgcct
     3361 gccccgcgtt gccggtgacg ccgacaaccg atgtgatgcc gatgattcgc ccgaagcgcc
     3421 ggcgcatcat gccgcgcagc gccgcccgcg ccagcacgaa accggccgaa aggttgaccg
     3481 cgagcacctg atcccactcc tcgtccttca tgcgcatggc gaggttatcc cgggtgatgc
     3541 cagcgttgtt gacgagaatg tccacctgcc ccatcgccgt ttccgccgcc ggcagcagcg
     3601 cctcgacgga cgcgcggtca gaaaggttgg cggtcgcgac atgaacgcgg tcgccaagcc
     3661 tttccgccag cttgtccagc gcctcgcgcc gcgtgccgga gagggtcacc accgcgccct
     3721 gcgcatgcag cgcctcggca atcgccgcgc cgatgccgcc cgacgcgccg gtgacgagcg
     3781 cattcttccc cgcaagactg aacatggaat gggtcctcta agccgtttgc ccgcctccat
     3841 acgtgaggcg caggtaaaag ggcaaggtgt gcttgaggcg gcgggctaca accgccattg
     3901 cgaggagcgt agcgatgaag caatccagcc tgccactttc ttttctggat tgcgtcgtca
     3961 ggcggacgcc ctcctcgcaa tgacggtgga ggccaagtcg tgaatggccg gtccaagccc
     4021 ggccatgacg gacgcggtgt tgcggatcgg cgttgtctca gccctgatac cccgccacat
     4081 cctccggcgt gccgacgttt gcgccggtcg cgccttcgac gatgcgcttg acgaggccgg
     4141 agagcacctt gcccgcgccg atctcgacga agcgggtgac gcccttggtc cccagccagg
     4201 ccatgctctc ggcccagcgc accgatccgg tgacctgttc gacgaggcgc gcgcggattt
     4261 ccgccgggtc cgtgatggcg ctggccagaa cattggcgac caccggcacg ttcgggacgt
     4321 tgatggtcgc cttcgccagc gcttcggcca tcgcctcggc ggcgggctgc atcagcgcgc
     4381 agtggaacgg cgcggagacg ggcagcggca tcgcgcggcg tattcctttt gagggagcca
     4441 gcgcgatggc gcggtcgatg gccgctttcg taccggatat caccacctgc ccgccgccgt
     4501 tgtcgttggc gacggcgcac acctcgcctt gc
//
LOCUS       DAOMXN010000206         4514 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_10313_length_4514_cov_10.018838, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000206 DAOMXN010000000
VERSION     DAOMXN010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4514
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10313_length_4514_cov_10.018838"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            283..939
                     /locus_tag="PK812_11985"
     CDS             283..939
                     /locus_tag="PK812_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017484188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRJ70313.1"
                     /translation="MPEVIFNGPAGRLEGRYQPAKQRGAPLAIVLHPHPQFGGTMNNQ
                     IVYNLYYTFADRGFSVLRFNFRGVGRSQGVFDHGQGELSDAASALDWVQALNPDAKTC
                     WIAGISFGAWIGMQLLMRRPEIEGFISIAAMANRYDFSFLAPCPSSGLFVHGDQDRVA
                     PVAEVINVIDKVKTQKGVKIEHAVIKGANHFFENHVDELIGTVGAYLDRRLTSKGRAA
                     "
     gene            complement(986..1273)
                     /locus_tag="PK812_11990"
     CDS             complement(986..1273)
                     /locus_tag="PK812_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70314.1"
                     /translation="MRHISKISGVFFAVLLLPTVVAAQSAQQTFDQNMKDAQKAYERQ
                     KNESDKQKMKEYQKDAGNFVPGTLDKKIGKDQYISPSYKNGAGGGTYTKTF"
     gene            complement(1389..2351)
                     /gene="lipA"
                     /locus_tag="PK812_11995"
     CDS             complement(1389..2351)
                     /gene="lipA"
                     /locus_tag="PK812_11995"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HRJ70315.1"
                     /translation="MPATIDLLTNDPRKARDEALRHPEKAHRPDNPIQRKPDWIRVKA
                     PGSPKWAETRNIVKENKLVTVCEEASCPNVGECWEHKHATFMIMGDTCTRACAFCNVK
                     TGLPEALDPNESAHIGDATAKLGLKHVVITSVDRDDLADGGAQHFADVIREIRARSPG
                     TTIEILTPDFLRKEGALEVVVKARPDVFNHNLETVPSKYLKVRPGARYFHSIRLLQRV
                     KELDPTIFTKSGIMVGLGEERNEVLQLMDDLRSADVDFLTIGQYLQPTKKHHAVIRFV
                     PPDEFKGYETIAYAKGFLMVSATPLTRSSHHAGSDFERLRAAKR"
     gene            complement(2418..3857)
                     /gene="lpdA"
                     /locus_tag="PK812_12000"
     CDS             complement(2418..3857)
                     /gene="lpdA"
                     /locus_tag="PK812_12000"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015915931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRJ70316.1"
                     /translation="MSAYDVIIIGSGPGGYVTAIRSAQLGFKTAIVEREHLGGICLNW
                     GCIPTKALLRSAEIYHFATHPEAYGLKVEGTVTPDVAAIVKRSRGVSQRLNGGVGFLM
                     KKNKIDVIWGEAKLSKPGEIVVGKSSKAPHQPQHPAPKGVAGEGTYTAKHIIVATGAR
                     PRALPGIEPDGKLIWTYFEAMVPPAMPKSLIVMGSGAIGIEFASFYRTLGCDVTVVEV
                     MPTIMPVEDVEISAIARKAMEKQGMKIHTSTKVIKVAKGADSVTATIEDDKGNKKDVT
                     AERMISAVGVVGNIENLGLEALGVKTDRGCIVIDGYGRTNVPGVYAIGDVAGPPMLAH
                     KAEHEGVICVEHIKGLHVHPMDKAKIPGCTYCHPQVASVGLTEAKAKEAGYQVKVGRF
                     SFGANGKAIALGEDNGLIKTVFDARTGQLLGAHMVGAEVTELIQGFVVAMNLETTEEE
                     LMHTIFPHPTLSEMMKESVLDAYGKVLNA"
     gene            complement(3897..4247)
                     /locus_tag="PK812_12005"
     CDS             complement(3897..4247)
                     /locus_tag="PK812_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HRJ70317.1"
                     /translation="MKARAKHMRVAMTDHERRLWLALKQRLPMAGTHFRRQFAIGPYI
                     VDFCCLAHRLIVEVDGEQHGTDDARRYDERRTSYLTSQGFRVLRFWNHEVTREMESVL
                     DTILASIHGGHPHP"
BASE COUNT          828 a         1401 c         1340 g          945 t
ORIGIN      
        1 gccgccacgt ccgggcggat cggcgccgtc gcattccagt cgcaatatgc gcgtggcgcg
       61 ggcatccggg gctccctcaa actgtacgca aaatcacaac ttttgcgtgc agtttagaac
      121 ttgtctaacg gctacatagg agttccgatt gacagacgtc aagtcacccc ggcggcaaat
      181 ctgccgctgc acgagcctgc ctcggccggg acgctgattt tccttggctt tgcgctggct
      241 ggtcatgcta gaaggcgccc ggtttctcag tgggataacg tgatgcccga agtcattttc
      301 aacggccccg ccggccgcct cgaaggccgt taccagcccg ccaagcagcg gggcgcaccg
      361 ctcgccatcg tgttgcatcc gcatccgcaa ttcggcggca cgatgaacaa ccagatcgtc
      421 tataacctct actacacgtt tgccgaccgt ggcttttcgg tgttacggtt caatttccgc
      481 ggggtcggac ggtcgcaggg cgtgttcgat catggccagg gcgaactttc tgatgccgcc
      541 tcggcgctcg actgggtgca ggcgctcaac cccgatgcca agacctgctg gattgccggc
      601 atttccttcg gcgcctggat cggcatgcag ctgctgatgc ggcgcccgga aatcgagggg
      661 ttcatctcca ttgcggcgat ggccaatcgc tacgatttct cgttcctcgc cccctgcccg
      721 tcttccggcc tgttcgtcca tggcgatcag gatcgcgtcg caccggttgc cgaggtcatc
      781 aacgtgatcg acaaggtcaa gacgcagaag ggcgtcaaga tcgagcatgc ggtgatcaag
      841 ggcgccaacc acttcttcga gaatcatgtc gacgagctga tcggcacggt cggcgcctat
      901 ctcgaccgcc gcctgacctc gaagggccgc gcggcgtaat actttacgcc tcggctgtca
      961 gttgcagcca aggtcataaa agttttcaga acgtcttggt ataggtccca ccaccggcgc
     1021 cattcttata tgacggcgat atatactggt cctttccaat tttcttatca agagtcccgg
     1081 gaacaaaatt cccggcatcc ttttgatatt ctttcatttt ttgcttatcc gactcgtttt
     1141 tctgcctctc gtatgctttc tgtgcatcct tcatattttg gtcaaatgtt tgctgagcag
     1201 actgcgccgc cacaacagtt ggcaacaaca gaacagcaaa aaaaactccc gaaatcttag
     1261 atatgtgtct cataatacgt gccctcctcg acaaaaagca aacgtctatt tcttactgta
     1321 aacagaaata tgaaaattgc cgtcacccaa gtgaatgacc gtaatattca aatttttaaa
     1381 cttgataatt accgcttcgc ggcgcgcagc cgctcgaaat cgctgcccgc gtgatgcgac
     1441 gagcgggtca acggcgtggc cgagaccatc aggaagccct tggcataggc aatcgtctca
     1501 tagcccttga actcgtccgg agggacgaag cggatcacgg cgtgatgctt cttcgtcggc
     1561 tggagatact ggccgatggt gaggaaatcg acatcggccg acctgagatc gtccatcagc
     1621 tgcaggactt cgttccgctc ctcgccgagg ccgaccatga tgccggattt ggtgaagatg
     1681 gtggggtcga gttccttcac ccgctgcaac aggcggatgg aatggaaata gcgggcgccg
     1741 ggccgcactt tcagatattt cgacggcacg gtttcgaggt tgtggttgaa cacatcgggc
     1801 cgggctttca ccaccacctc cagcgcgccc tccttgcgca ggaagtcggg tgtcaggatc
     1861 tcgatggtgg tgccgggcga gcgggcacgg atttcgcgga tgacatcggc aaagtgctgt
     1921 gcgccgccat cggcgagatc gtcgcggtcg accgaggtga tgaccacatg cttcagcccc
     1981 agcttggcgg tggcgtcgcc gatatgggcc gactcgttgg ggtcgagcgc ctcgggcagg
     2041 ccggtcttga cgttgcagaa ggcacaggcc cgggtgcagg tgtcccccat gatcatgaag
     2101 gtggcgtgct tgtgctccca gcattcgccg acattcgggc agctcgcctc ctcgcacacc
     2161 gtgacgagct tgttctcctt cacgatattg cgcgtctcgg cccatttggg cgaacccggc
     2221 gccttgacgc gaatccagtc cggcttgcgc tggatcggat tgtcgggccg atgcgccttt
     2281 tcagggtgac gcagagcctc gtcgcgcgcc ttgcgcggat cgttggtcag aaggtcgatg
     2341 gtggcgggca tggctgtctc gctttaagga aaccgcaata gcggttgaac cggcgggtgg
     2401 aaagccccgc cgcggactta cgcgttcagc actttgccat aggcatccag cacgctttcc
     2461 ttcatcatct ccgacagggt cggatgcggg aagatggtgt gcatcagctc ctcttccgta
     2521 gtttcgaggt tcatcgccac gacgaagccc tggatgagtt ctgtcacttc cgcacccacc
     2581 atatgggcgc ccagaagctg gccggtcctg gcgtcgaaca cggtcttgat caggccgttg
     2641 tcctcgccga gcgcgatcgc cttgccattc gcaccgaacg agaagcggcc gaccttgacc
     2701 tgatagcccg cctccttggc tttcgcctcg gtcaggccga cgctcgccac ctgcgggtgg
     2761 caataggtgc agcccggaat cttggccttg tccatcgggt ggacgtgcag gcccttgatg
     2821 tgctcgacgc agatcacgcc ctcatgctcg gccttgtggg cgagcatcgg cgggccggca
     2881 acgtcgccga tggcatagac gcccggcaca ttggtgcggc catagccgtc gatgacgatg
     2941 cagccgcggt cggtcttgac gcccagcgcc tccagtccga ggttctcgat gttgccgacg
     3001 acgccgacgg ccgaaatcat ccgctcggcg gtgacgtcct tcttgttgcc cttgtcgtcc
     3061 tcgatggtgg cggtgacgct gtcggcgccc tttgccacct tgatcacctt ggtggaggtg
     3121 tggatcttca tcccctgctt ttccatcgcc ttgcgggcga tggcggaaat ctcgacatcc
     3181 tcgaccggca tgatcgtcgg catcacctcg actacggtca catcacagcc gagcgtcctg
     3241 tagaacgagg cgaactcgat gccgatggcg cccgagccca tcacgatcag cgacttgggc
     3301 atcgccggcg gcaccattgc ctcgaaatag gtccagatca gcttgccgtc cggctcgatg
     3361 ccgggcagcg cgcgcggccg cgcaccggtg gcgacgatga tgtgtttggc ggtgtaggtg
     3421 ccctcgccgg ccacgccctt cggcgccgga tgctggggct gatgcggcgc tttcgatgac
     3481 ttgccgacga cgatctcgcc gggtttggag agcttcgcct cgccccagat gacatcgatc
     3541 ttgttcttct tcatcaaaaa gccgacgccg ccattgagcc gctgcgacac tccgcgcgag
     3601 cgcttgacga tggccgcgac atccggcgtc accgtgccct cgaccttgag gccataagct
     3661 tccggatggg tggcgaaatg atagatctcg gccgagcgca gcagcgcctt ggtcgggatg
     3721 cagccccagt tgaggcagat gccgcccaga tgctcgcgct cgacgatcgc cgtcttgaag
     3781 ccgagctgcg ccgagcggat ggcggtgaca taaccgccgg ggcctgagcc gatgatgatg
     3841 acgtcgtaag cagacatgcg cctctcctgc attcccctcc cccttgctgg gagtggctag
     3901 gggtgggggt ggcccccgtg aattgaagcg agaatggtgt cgagcaccga ttccatctcg
     3961 cgggtgactt cgtggttcca gaaacgcaaa acgcgaaagc cttgcgatgt cagatagctt
     4021 gtgcgccgct catcgtagcg ccgtgcgtcg tctgttccgt gctgctcgcc atcaacctcg
     4081 acaatcagac gatgcgccag gcagcagaaa tcaacgatgt aggggccgat ggcgaattgc
     4141 cgtcggaagt gcgtgccggc catcggcaag cgctgtttca gagcaagcca gagccggcgc
     4201 tcgtgatcgg tcatggcgac gcgcatatgc ttcgcccgag ctttcacgac cgcagaaatt
     4261 cctgtttccc gttcgctccg ccaagccaaa accagccacc cccatccccg gcccttcccc
     4321 gcagggggga agggagaaac tcaaacctcg ccgctttcct ccctccccct tgcggggagg
     4381 gtgtggcgaa gcgacgggtg ggggtggttc aacagacgcg gaagtcaaag caataactca
     4441 acctcccgca tccagaaccg ccacccccat ccccggccct tccccgcagg ggggaaggga
     4501 gaaactcaaa cctc
//
LOCUS       DAOMXN010000207         4484 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_10415_length_4484_cov_9.683450, whole genome shotgun sequence.
ACCESSION   DAOMXN010000207 DAOMXN010000000
VERSION     DAOMXN010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10415_length_4484_cov_9.683450"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="PK812_12010"
     CDS             complement(<1..685)
                     /locus_tag="PK812_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ70318.1"
                     /translation="MSETSVIPFPDRFDATSLQDKGLAALLKLANSARESLQLDQARR
                     AYEHILSFDPDNLDARIGLMRCHFYAENWPEAFKALEVRFQVMGDPPQVFVTDRKGNR
                     RAKPRWTGGPAPGSLLVLDEQGMGDTLQFMRFLPRLISGGTRVTLVTHRRLFALIRAA
                     GIACELRPMDQAGSVTGIEAWSPLLSLPMAMGLGAADIPMRRPYLAVDPARVARWRAW
                     LGPAGLKVGV"
     gene            773..2047
                     /locus_tag="PK812_12015"
     CDS             773..2047
                     /locus_tag="PK812_12015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019997728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRJ70319.1"
                     /translation="MAGQRAPALLRAISMKILFVHQNFPGQWIHLARHYKQEGHEVVA
                     IRDAANERQDFLPTAAYTFTLPKMPSPHPVPFAAHFEERVARAEAVGVAGLELKKRGF
                     EPDLIAGHGGWGETLVLREVWPKARQILYAEFCYRTSGADTDFDPEFGRPSMRAHFNT
                     AAKNAVMFTALAYADRGLAPTFWQRDGFPAHMRDHIEVIHDGIDTDLVRPAPDSHVTL
                     NDGAVVLKPGDEVITFVSRNLEPYRGYHIFMRALPKILAERPKARVIIVGGDGVSYGR
                     AAPEGQSWKDIFLNEVKDRLDLSRVHFVGRIPYSTYLNVLHVSAVHVYLTYPFVLSWS
                     MLESMAAGCIVVGSRTPPVQEVIEHGRNGLLVDFFDPDGLANMVTSVLKRPAKYRHLR
                     HAARETAVKRYDLKTVCLPQLCQFFEKTAAGG"
     gene            2194..2571
                     /locus_tag="PK812_12020"
     CDS             2194..2571
                     /locus_tag="PK812_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HRJ70320.1"
                     /translation="MAIDELNQPFTKAEMEAMRRSARDENRLLAEAFALLKRVARRIP
                     FAEDALALYYCARDPMTETRVKLIVLAALAYLVLPLDTIPDMLPLMGFADDAAVIAAA
                     IAAVRGAISDLHRRKARDTLAGF"
     gene            2645..3193
                     /locus_tag="PK812_12025"
     CDS             2645..3193
                     /locus_tag="PK812_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HRJ70321.1"
                     /translation="MKRILPVLALAMLVASPALAQQKKKEEPKAAAGPTGGGTFQIAT
                     YGDWGVYTSGKDKQKVCYALSQPKDRMPKGLNRDPAYVFISNRPGDGARNEFSIIMGY
                     PLKLNGAATAAVGNASFSMLAKDKSAWLKNAAEESQLLDAMRKGKDLIVKGTSAKGNE
                     TTDRYSLSGLGQAIERAQKECP"
     gene            complement(3306..3971)
                     /locus_tag="PK812_12030"
     CDS             complement(3306..3971)
                     /locus_tag="PK812_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008838627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HRJ70322.1"
                     /translation="MSSLLTADALSALFQVIMIDLVLAGDNAVVIGLAVAGLPAEMRS
                     KAILLGIAAATVLRIIFAALTTQLLSVIGLLLAGGVLLLWVCWKMWRELRTPHEQEVE
                     AEEALEGADLDADGKVAGRAPRKTFKQAAWQIIIADVSMSLDNVLAVAGAARDHPTIL
                     VIGLVFSIALMGLAANYIARLLLHHRWIAYVGLAIILYVAGEMIYRGALEVWPYLNGA
                     ART"
BASE COUNT          789 a         1534 c         1470 g          691 t
ORIGIN      
        1 cgaccccgac cttcaggccc gccggaccga gccaggcacg ccagcgcgcg acgcgcgccg
       61 gatcgaccgc aagataggga cgccgcatcg gaatgtcggc cgcgccaaga cccatcgcca
      121 tcggcagact gagcaggggc gaccatgcct cgatcccggt gacgctgccg gcctggtcca
      181 tcggccggag ctcgcaggcg atgcccgcgg cccggatgag ggcgaacagc cggcggtgcg
      241 tgaccagcgt gaccctggtg ccgccggaga tcagacgcgg caggaaacgc atgaactgga
      301 gggtgtcgcc catgccctgt tcgtcgagca ccagcaggga accgggcgcc ggcccgccgg
      361 tccagcgcgg cttggcgcgc cggttgccct tgcggtcggt gacgaagacc tgcggcggat
      421 cgcccatcac ctggaaccgc acctccaacg ccttgaaggc ttccggccag ttctcggcgt
      481 agaaatggca gcgcatcagg ccgatccgcg cgtcgaggtt gtcgggatcg aagctgagga
      541 tgtgctcgta ggcgcgcctc gcctggtcga gctgcagcga ttcccgggcg gaattggcca
      601 gtttcagcag ggccgccaga cccttgtcct gcaacgacgt cgcatcgaag cgatcgggaa
      661 agggaatgac ggaagtctca gacatggcag gttcgtttcg cgtccgggct ttcggaaggt
      721 ggccgcttcc ggcggattaa cgaaatagaa cccatgatcc ggttaagatt ccatggccgg
      781 ccagcgcgcg ccggcgctgc tgagagcgat ttccatgaag atcctgtttg tccaccagaa
      841 ctttcccggt caatggattc atctggcgcg ccactacaag caggaaggcc acgaggtcgt
      901 cgccatccgc gacgcggcga acgagcgcca ggacttcctg ccgactgcgg cctacacgtt
      961 caccctgccg aagatgccgt cgccccatcc cgtgcccttc gccgcgcatt tcgaggaacg
     1021 cgtggcgcgg gccgaagccg tgggcgtcgc cgggctggag ttgaagaagc gcggcttcga
     1081 gcccgatctg atcgccgggc acggcggctg gggcgagacg ctggtgctgc gcgaggtctg
     1141 gccgaaggcg cggcagattc tctacgccga gttctgctat cgcacgagcg gcgccgacac
     1201 cgacttcgac cccgagttcg gccggccgtc gatgcgggcg catttcaaca ccgccgccaa
     1261 gaacgccgtg atgttcaccg cgctcgccta tgccgatcgc ggcctggctc ccaccttctg
     1321 gcagcgggac ggctttccgg cgcacatgcg cgaccatatc gaggtgatcc acgacggcat
     1381 cgacaccgat ctggtgcgcc ccgctcccga cagccacgtc acgctcaacg acggcgccgt
     1441 ggtgctgaag cccggcgacg aggtgatcac cttcgtctcg cgcaatctcg aaccctatcg
     1501 cggctaccac atcttcatgc gcgccctgcc caagatcctg gcggagcgac cgaaggcccg
     1561 cgtcatcatt gtcggcggcg acggcgtctc ctacggccgc gcggcccccg agggccagag
     1621 ctggaaggat atattcctga acgaagtgaa ggaccggctc gacctctcac gcgtgcactt
     1681 cgtcgggcgc atcccctact cgacctacct caacgtgctg cacgtgtccg cggtgcacgt
     1741 ctacctgacc tatccattcg tcctgtcctg gtcgatgctg gaatcgatgg cggcgggctg
     1801 catcgtcgtg ggatcgcgca cgccgccggt gcaggaggtg atcgaacatg gcaggaatgg
     1861 cctgctggtg gatttcttcg atcccgacgg gctcgccaac atggtgacca gcgtgctcaa
     1921 gcgcccggcg aagtaccggc acctgcgcca tgccgcccgc gagaccgccg tcaagcgcta
     1981 cgacctcaag acggtctgcc tgccgcagct ctgtcagttc ttcgagaaga ccgcggcggg
     2041 aggctgaccg agcgctgcgc cgcccgggca ggcctggttc caccgtggcg ccggcgggtt
     2101 gcggatgccg cccaccagca tcctggacag gacgccactt gcatgccgca atttcgatgt
     2161 tcatctagtg gcggtcctgt tccggagagc ggcatggcga ttgacgagtt gaaccaaccc
     2221 ttcaccaagg ccgagatgga ggcgatgcgc cgctccgcgc gtgacgagaa tcgcctgctg
     2281 gccgaagctt tcgcgctgct gaagcgcgtc gcccggcgca ttcccttcgc cgaggacgcg
     2341 ctggcgctct actattgcgc ccgggacccc atgaccgaga cgcgggtcaa gctgatcgtg
     2401 ctcgccgccc tcgcctatct ggtgctcccg ctcgacacga tcccggacat gctgccgctg
     2461 atgggcttcg ccgatgatgc ggcggtcatc gccgccgcga ttgccgccgt gcgcggcgcc
     2521 atctcggatc tgcaccgccg caaggctcgc gacacgctgg ccgggttctg atccggagca
     2581 tttgccggct gcgccgtcct cagctaggag aagcagtccc ttgcgaagcc cttttgagtc
     2641 cgcgatgaag cgcatcctcc ccgtactcgc ccttgccatg cttgttgcca gtcctgccct
     2701 ggcgcagcag aagaagaagg aagagccgaa agccgccgcc ggcccgacgg gcggcggcac
     2761 gttccagatc gccacctacg gtgactgggg cgtctacacc agcggaaagg acaagcagaa
     2821 ggtctgctat gcgctgtcgc agccgaagga ccgcatgccc aagggcctca accgcgaccc
     2881 ggcctatgtc ttcatttcca accggccggg cgacggggcg cgcaacgaat tctcgatcat
     2941 catgggctat ccgctcaagc tcaacggcgc ggcgacggcg gccgtgggca atgcctcgtt
     3001 ctcgatgctg gccaaggaca agagcgcctg gctgaagaac gcggccgagg aaagccagct
     3061 gctggacgcc atgcgcaagg gcaaggacct gatcgtcaag ggcacctcgg ccaagggcaa
     3121 cgagacgacc gaccgctatt ccctcagcgg gctcggccag gcgatcgagc gcgcccagaa
     3181 ggaatgtccc tagaccagcg gcgtgacccg tcgcccttcc ggatcacgat ggccgaccca
     3241 cgatcaggcg accttgggcg cacggcgatt tccgatgggc tcgatgccgg ttgcatcgag
     3301 catcgtcagg tgcgcgccgc gccgttcagg taaggccaca cttcgagcgc gccgcgatag
     3361 atcatctcac cggccacata gagaatgatc gcgaggccga cataggcgat ccagcgatga
     3421 tgcagcagca gtcgggcgat gtagttcgcc gcgaggccca tcagcgcgat cgagaaaacg
     3481 aggccgatga ccaggatggt tggatggtcg cgcgccgcgc cggcgacggc cagcacgttg
     3541 tcgagcgaca tcgagacgtc ggcgatgatg atctgccagg cggcctgctt gaaggtcttg
     3601 cgcggcgcgc gcccggccac cttgccgtcg gcgtcgaggt cggcgccttc cagcgcctct
     3661 tccgcctcga cctcctgttc atgcggggta cgcagttcgc gccacatctt ccagcacacc
     3721 cacagcagca gcacgccgcc ggccagcagc aggccgatga ccgagagaag ctgggtggtg
     3781 agcgcggcga agatgatgcg caaaaccgtg gccgcggcga tgccgagcag gatcgccttg
     3841 gagcgcattt ccgccggcag gccggcaacg gcaaggccga tgaccaccgc gttgtcgccg
     3901 gccagcacca ggtcgatcat gatgacctgg aacagggcgc tgagcgcatc ggcggtcagg
     3961 aggctggaaa tgaagtccat gtgaaaacct gtcgtatcga tgcacccgga gaacgatccg
     4021 gtgctggctc ggacgagcgg cgagcgggcg acgggccgtg aatggcaggt cctataccgc
     4081 gcggcagcgg agccgaaaag gggagaaacg cccaaacggc acaattgccg cctgcatgcg
     4141 gtgcataccc tcctccccgc agccgcaccg cccggaagcc aaggcaaccg aagccgatgt
     4201 cccgccgtat caggccggag acatcccgca atccgatcag gcgccatcgg gaccgggcgc
     4261 acccgggctg cgacgcagac ccctcccgct gaccgtccaa ccgtgctaag cggccgccca
     4321 tgaccgcatc ctccccgctt gcagccccga tcgagaagac cgccgccgcg tccgcccctg
     4381 ccgcgctggt gtcggccgac gcccggccct cgctggtcgg actgacccgc gccgcgctgg
     4441 cggagcggct tatggcggtg ggggttgagg ccaagcctgc gcgt
//
LOCUS       DAOMXN010000208         4437 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_10557_length_4437_cov_7.828845, whole genome shotgun sequence.
ACCESSION   DAOMXN010000208 DAOMXN010000000
VERSION     DAOMXN010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4437
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10557_length_4437_cov_7.828845"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="PK812_12035"
     CDS             complement(<1..445)
                     /locus_tag="PK812_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRJ70323.1"
                     /translation="MSNRERRRALIADDHGLYRMGLAFLLKDRLGLDEVIEVGGFDDA
                     LDRLAANPDIAMALFDLSMPGMGGPESLDVVKETYPDVKVAVVSGSEEREHVLKAVSI
                     GLSGYVPKGLSEDEIAKALQLILDGHVYIPVFMTAAAKPRSARPEA"
     gene            complement(554..2881)
                     /locus_tag="PK812_12040"
     CDS             complement(554..2881)
                     /locus_tag="PK812_12040"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRJ70324.1"
                     /translation="MTKPFNLRSLLTVAISALALFLVALATWTIGQAGARRVEAEIGA
                     SMQLQADQMQDKLDRALFRRYREIGIAARMLPFIHGADQAKGVQIWLDELRKSNSNYS
                     WIGVTDAGGTIIASTDEGSVGRSAAAGSWFLAAASGQTFGELDHDELRLSGPAGVEGG
                     IGRPVFVSAPILDDRGSSIGVVAAILDWGWVDEVKDSLFGSFASAREEELILLNSARE
                     VLIGPSDLVGKPLSLPSVEAALRGKNHFASEPWPDGGTYVTGYARSDGYADFAGLGWI
                     VLVRQDAELALAPVRDLQRQTLTIGLVLAGLAAVVAWMLAHRASAPLLRLANAADAVR
                     RGADIPIPAIGDYAEAATLSRSLDAMVTELKHRQQALASLNETLESQVKERTAELARQ
                     NSALIVAHQEAEDATAAKSRFLAAASHDLRQPLHAMNLFARALSRRVLGAEATRLVSQ
                     LESSLASLKEMFDALLNVSRLDAGLIEVNASTVKVRDFMERIAEGFRAEASQRELKFV
                     TRPVDGEIVTDPVLLETILRNLLSNALKFTTRGGIVFSARRDGDRLAFDVFDTGKGIA
                     PDRQAGIFQEFERSKEHATGANDGLGLGLSIVRRYAQLLGIEVRLVSRPGRGTRFTLM
                     MPQAATAGVRPSAPDLANVAASTQLTGARIAIIDDDPQIVAGMERELQDRGCSALTFA
                     SIDDAREQLGQGLSLDAAIVDFDLGSGMTGPALLAAVEKMRGHPVAALILTGGTDAAT
                     LKTVIQTGRPWLTKPADPDAVAKAVVSLICSESPR"
     gene            complement(3004..4119)
                     /locus_tag="PK812_12045"
     CDS             complement(3004..4119)
                     /locus_tag="PK812_12045"
                     /inference="COORDINATES: protein motif:HMM:NF015500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HRJ70325.1"
                     /translation="MTVFSENISVDPNLRSKKPSRIWAWLSAPAGLALIGAALGFGVS
                     DAAKAQESRSARTLLADAKGWTYQLQGLDTGRAAVEAEDLLVIDYSRDGSAEGALRPA
                     EVEALKLKADGERRVVVSYLSIGEAESYRYYWQKGWSMVQAGAAGREAADAQKSVTEC
                     AADDDEGESALLPAPSGAKVPGPNAPGWLHYENVQWKGNYYVRFWEPEWQRIIFGTPT
                     SYLDRIIDAGFDGVYLDRADVYGFWKGAHATSEDDMVDFIAELSAYAKARNPDFLVIL
                     QNAEDLVRHEAVRDAIDSIAKEDLLFGIDHTETENCSQEVAASTDYLKFAQAQGKPIF
                     VVEYINDPGKQEAARARISELGFQAAFASRELDGFNQ"
BASE COUNT          781 a         1439 c         1437 g          780 t
ORIGIN      
        1 tcgcttccgg tcgcgccgag cgtggcttgg cagccgccgt catgaacacc ggaatgtaga
       61 cgtgaccgtc gaggatgagc tgcagcgcct tggcaatctc gtcctctgaa aggcctttcg
      121 gaacatagcc gctgagaccg atcgagacgg ccttcaggac gtgctcccgc tcctccgaac
      181 ccgacacgac ggcgaccttc acatcgggat acgtctcctt gaccacgtcc aggctttccg
      241 gcccgcccat cccgggcatc gacaggtcga acagcgccat ggcgatgtct ggattggcgg
      301 ccagccggtc gagcgcgtcg tcaaagccgc cgacctcgat cacttcgtcc agtccgaggc
      361 gatccttcaa caggaacgca agccccatgc gatagagacc atggtcatcg gcaatcaggg
      421 ctcgccgccg ctcgcgattg gacatctggg tgcactactt cctgcagttg cagcgggcag
      481 atgggatcgc gcggctccgg tcgcgctgca ggtttgcgca atcgccgcgc tcgctggtca
      541 acttgacgac cccctacctt gggctctcgc tacaaatcag cgagaccacg gcctttgcaa
      601 ctgcgtccgg atcggccggc tttgtcaacc atggtcgtcc agtctgtatc accgtcttga
      661 gtgtggccgc gtcggtgcca ccggtcaata tcagtgcggc gacagggtgg ccccgcatct
      721 tctcgaccgc cgcaagaagg gcaggccccg tcattccgga cccaaggtcg aaatcgacga
      781 tggcggcgtc gaggcttaat ccctgaccca gttgctcgcg agcatcatcg atcgacgcga
      841 acgtgagtgc ggaacagccg cggtcctgca gctcccgctc catgccggcg acgatctgcg
      901 gatcatcgtc gatgattgcg atccgggcgc cggtcaattg tgtcgacgcc gccacgttcg
      961 caagatctgg cgcggacggc ctcaccccgg ccgtcgccgc ttggggcatc atcaaggtga
     1021 accgagtgcc gcgtccaggc cgggatacca gccggacctc gatgcccagc aattgagcgt
     1081 agcggcggac gatcgaaagg ccgaggccca aaccgtcgtt ggcgcccgtg gcgtgctcct
     1141 tggaacgctc gaactcctga aagatgccgg cttggcggtc gggcgcgatg cccttgccgg
     1201 tatcgaacac gtcgaatgcc agccgatcgc catcgcgccg cgccgagaac acgatgccgc
     1261 ctcgcgtcgt gaacttcagg gcgttggaca acaggtttct caggatggtc tcgaggagga
     1321 cgggatcggt gacgatttcg ccgtcgaccg ggcgcgttac gaacttgagc tcgcgctgac
     1381 tggcctcggc ccggaatcct tccgcgatac gctccatgaa atctcggacc ttgacggtgc
     1441 ttgcgttcac ctcgatcaac ccggcgtcaa ggcgggacac attgagaagt gcgtcgaaca
     1501 tctccttcaa cgaagccagc gaggactcca gctggctgac aaggcgcgtc gcctcggcgc
     1561 ccaacacgcg ccgcgacagc gcacgcgcaa acaggttcat ggcatgcagc ggttgccgca
     1621 gatcgtggct tgcggcggcg aggaagcgcg atttcgcggc ggtggcgtcc tccgcttcct
     1681 ggtgagcgac gatcagcgcg ctattctgcc gtgcgagctc agcggttcgc tccttgacct
     1741 gtgactcgag tgtttcgttc agcgacgcca aggcctgttg gcgatgcttc aattctgtca
     1801 ccatggcgtc gagggacctc gacagggtcg ctgcttcggc atagtcgccg atggcgggga
     1861 tgggaatgtc ggcgccgcgg cggaccgcgt cggcggcatt cgcgaggcgc agcagcgggg
     1921 ctgacgctct gtgcgccagc atccaggcga caacggccgc aagcccggcc agtacaagac
     1981 ctatcgtcag cgtctggcgc tgcaggtccc gcacgggggc gagcgccagc tccgcgtcct
     2041 ggcgcacgag cacgatccag ccgagaccgg cgaagtcggc atagccatcg ctgcgcgcgt
     2101 agccggtgac gtaggtgccg ccgtcgggcc acggctcgct tgcgaagtgg ttcttgccgc
     2161 gcaaggcggc ttcgacgctc ggcaggctca gaggttttcc gacgagatcc gagggaccga
     2221 tcagaacctc gcgagcggag ttcaaaagga tcagctcctc ctcgcgagcg cttgcgaagc
     2281 ttccgaacag agagtctttc acctcgtcca cccaccccca gtcgagaatg gcggcgacga
     2341 cgcctatcga cgagccgcga tcgtcgagga tgggtgcgga gacgaaaacc ggtcggccta
     2401 ttcctccctc cacgccagcc gggccggaca atctcagctc gtcgtggtcg agttccccga
     2461 aggtctgccc gcttgcggcg gccaggaacc agctgccggc ggcggcgctg cgaccgacag
     2521 agccctcatc ggtcgaggcg atgatcgttc caccggcgtc ggtcacgccg atccatgaat
     2581 agtttgaatt gctcttgcgc aattcatcga gccagatctg cacgcctttc gcctggtcgg
     2641 cgccgtggat gaacggcagc atccgcgcgg cgatgccgat ttcgcggtaa cggcggaaca
     2701 gggcccggtc gagcttgtcc tgcatctgat cggcctgcaa ttgcatcgac gcgccgattt
     2761 ccgcctcgac acgtcgggcg ccggcctggc cgatcgtcca ggtggcgaga gcaaccagga
     2821 acaacgccaa agcagagatg gcgacggtga gcagggaacg gagattgaag ggttttgtca
     2881 catccaggtt ccagagcgat gcgagaatct acagcctcgg tcggttggcg caagatgtgc
     2941 ggaactcaga ctcggagccc ggctcaggtg agacgggctc cggtccgggg aggagaggca
     3001 gggctattgg ttgaagccgt cgagttcgcg ggatgcgaaa gcggcctgga agccgagttc
     3061 ggagatgcgg gctctcgccg cctcctgctt gcccgggtcg ttgatgtact cgaccacgaa
     3121 gatcggcttg ccctgggcct gcgcgaactt caggtagtcg gtgctggcgg cgacctcctg
     3181 ggagcagttc tcggtctcgg tgtggtcgat gccgaacagc aggtcctcct tggcgatcga
     3241 gtcgatggcg tcgcgaacgg cctcatgccg gacgaggtcc tcggcattct gcaggatcac
     3301 gaggaagtcg gggttccgcg cctttgcata agctgacagc tctgcgatga aatcgaccat
     3361 gtcgtcctcg gacgtggcgt gcgcgccttt ccagaatccg tagacgtccg cacgatcgag
     3421 atacacgccg tcgaagcccg catcgatgat gcggtcgagg tagctcgtcg gcgtaccgaa
     3481 gatgatcctc tgccactccg gctcccagaa ccgaacatag tagttgccct tccactgcac
     3541 gttctcgtag tgcagccagc ccggagcgtt gggcccaggc accttggcgc cggacggcgc
     3601 cggaagcagc gccgactcgc cctcgtcatc gtcggcagcg cactcggtca ccgacttctg
     3661 ggcatcggcg gcttcacggc ctgccgcacc ggcctggacc atgctccagc ccttctgcca
     3721 gtagtagcgg tagctctccg cctcgccgat gctgaggtag gagaccacaa cccggcgctc
     3781 gccatcggcc ttgagcttca gcgcctcgac ctcggccggg cgcaaagctc cctccgcgga
     3841 accgtcacgg gaatagtcga tcaccagaag gtcctcggcc tcgacggcgg ctcgccccgt
     3901 atcgagaccc tgcagctgat aagtccagcc cttcgcgtcg gcgagcagcg tcctggccga
     3961 acggctttcc tgcgctttgg cggcgtcgga cactccgaag ccgagtgctg cgccgatgag
     4021 ggccaagccg gccggcgcgg aaagccaggc ccagatgcgt gacggctttt tggaccgcag
     4081 attgggatcg acggaaatat tctcgctgaa aacggtcatc gctcggtctc cttcatgtga
     4141 tgagcatgga gacagaggta tctgcagccg gcgccctgca atactcggca cgatgcacta
     4201 tgaccgacta ggccgataaa cctaggccat catgtgcccg ggctccggcc acgaaccgcg
     4261 tcgggacacg agcggtgcgt cggatcgtca gcattcgcgg ttgtccggat gcctgcccct
     4321 tgagggtggc gacggctacg ccgcagagaa accggacgcc ggcgccgcgc atcaggtctt
     4381 cgatcaggca cccgggatgc cgatggggcg gcgcggggca aggtgacggc ggcgggc
//
LOCUS       DAOMXN010000209         4419 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_10620_length_4419_cov_10.743584, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000209 DAOMXN010000000
VERSION     DAOMXN010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4419
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10620_length_4419_cov_10.743584"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="PK812_12050"
     CDS             complement(<1..184)
                     /locus_tag="PK812_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70326.1"
                     /translation="MLRLLVPTLCLLALPAQAQKRPHLCASGETPAFSCATGRKVASL
                     CATSDADGATSALTYRF"
     gene            306..1787
                     /locus_tag="PK812_12055"
     CDS             306..1787
                     /locus_tag="PK812_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009492252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRJ70327.1"
                     /translation="MTKKSLPFLAFAALIAAAPGALAQNRDAAVLCMTLEPPILDPTA
                     GAAQAIKEVTYNNVFETLVSIDSASKLQPRLAENWVLAPDNVTYTFKLKSGVKFHDGA
                     EFTSADVKFSFERAVAPNSKNAQKASFTPIETITTPDPLTVVIKLKQPTARFLEGIAI
                     GDASIVSEKSAATNETRPVGTGGYRYVDWRRGDRLIYTRNEAYWGRKPAIKDVSYRFI
                     ADAQAQFAAIQAGDCDAITNFGAYESVDALRKNPALKVMVGSTEGETMLAINNAKAPF
                     NDLRVRRALAHAIDRKAVIEGAMNGVGVPIGSHFSPAHPAYVDLTGAVPYDPAKAKAL
                     LREAGFPNGFSTTLRLPPPPYARRSGEVIAAMLGEVGIKVSIEPVEFPQWLERVFRNK
                     EYDLSIIAHTEPLDVGNYARTDYYWGYNNADFQAMNTRANAEMDEAKRFQIYRDMQKK
                     ILDDQVNVFLFMLPKTTVAKKDLEGMWQNWPIPVSPASELSWK"
     gene            1873..2817
                     /locus_tag="PK812_12060"
     CDS             1873..2817
                     /locus_tag="PK812_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ70328.1"
                     /translation="MLSFILRRVAALALTLCVTAAVIFIVLEILPGDPAAVMMGVNAT
                     PENVAAIRAEYGLDRPAVERFLTWIGGLLTGDLGLSYTYRVPISSLIAERAQVTLPLA
                     AMAVMLSASIGIPLGTLAAAKANTRTDSLVMGISQLGLAVPNFWFGILLVLLFAVTLG
                     WFPAGGFPGWGAGVLAGIKTLILPAVALALPQSAILARLTRSAVLEALQEDFVRTARA
                     KGLSWPATLRKHVLRNALVPVVTILGLQFSVLVAGAIIIENVFSLPGLGRLMFQSIAQ
                     HDIIVVKDLVVLFAALVVFMNFIVDVAYGIIDPRLRRT"
     gene            2818..3687
                     /locus_tag="PK812_12065"
     CDS             2818..3687
                     /locus_tag="PK812_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009757560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ70329.1"
                     /translation="MSGPETIAAAPSFRRSPWRHPSLIIGGIIVALIVTAALVSLVWT
                     PHPPTKLNIPSRLQGPSATFWLGTDHFGRDVFSMLMVGARNSLAVGIVAVSIGAVFGV
                     LLGTASAARSGSWLDEAIMRTADFTFAFPAVLSAVMITALAGPGVVNAIIAIGIYNIP
                     IFARLTRSGALQVWSRDYVMAARAAGKTAAQITMRHVIPNIAGLIIVQATIQFATAIL
                     AEAGLSYLGLGTQPPNPSWGRMLFEAQTTMQRQPLLAIFPGLTIVVAVLGCNLLGDGL
                     RDVLDPRVNRSRS"
     gene            3710..>4419
                     /locus_tag="PK812_12070"
     CDS             3710..>4419
                     /locus_tag="PK812_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ70330.1"
                     /translation="MLLSVDTLTIGFGRKDAPIRVVRDLSFALEKGQTLGIVGESGSG
                     KTITALSLMGLQASAAHVSGSIRFEGEELLPHAEERMTDIRGKSIAMIFQEPMTALNP
                     VHRIGDQIAEGLILHEGFSQAAGLAEAVRLLERVGIAQAQERIAAYPHELSGGQRQRV
                     MIAMALACRPELLIADEPTSALDVTVQAQLLDLIRDVVAERGMALIVISHDLGIVTEL
                     AQRTIVLYGGAVMEEGETV"
BASE COUNT          725 a         1424 c         1512 g          758 t
ORIGIN      
        1 cgaagcgata ggtcagggcg ctcgtcgcgc catcggcgtc ggaggtcgcg cagagggagg
       61 cgaccttccg gccggtcgcg caggagaagg ctggcgtctc gccggaagcg cagagatgcg
      121 gacgcttctg cgcctgcgcg ggcagggcga gcaggcagag ggtcgggaca aggagacgga
      181 gcatggccca ttatgcccaa ccgcgacaac ggcggccagc ggcagaagtc actcttcccc
      241 ttttcgttgt cacaaagcgc cgctaagctc cgcaaagtcc ttggcttgtc catcggggga
      301 gcaccatgac caagaagtcg ctgccgtttc tggcgttcgc cgccttgatt gccgccgctc
      361 ccggggcgct ggcgcagaat cgcgatgcgg cggtgctgtg catgacgctg gagccgccaa
      421 tccttgaccc gaccgccgga gcggcgcagg cgatcaagga agtgacctac aacaacgtgt
      481 tcgagaccct ggtttcgatc gattccgcct cgaagctgca accgcgcctc gccgaaaatt
      541 gggtgctggc tccggacaac gtgacctata cgttcaagct caagtccggc gtcaagttcc
      601 atgacggggc ggagttcacc tcggcggatg tgaagttttc cttcgagcgg gcggtggcgc
      661 cgaattcaaa gaacgcacag aaggcgagct tcacgccgat cgagaccatc accacgcctg
      721 acccgctgac cgtggtgatc aagctgaagc agccgacggc ccgcttcctc gaaggcatcg
      781 ccatcggcga cgcctccatc gtcagcgaga aatcggccgc gaccaacgag accaggccgg
      841 tcggcacggg cggctaccgg tatgtcgact ggcggcgcgg cgaccggttg atctataccc
      901 gcaacgaggc ctattggggc cgcaagccgg cgatcaagga cgtgtcctac cgcttcatcg
      961 cggatgcaca ggcccagttc gcggcgatcc aggccggcga ttgcgacgcg atcaccaatt
     1021 tcggcgccta tgaaagcgtt gatgcgctga ggaagaatcc ggcgctcaag gtcatggtcg
     1081 gttcgaccga aggcgagacg atgctggcca tcaacaacgc caaggcgccc ttcaacgacc
     1141 tgcgcgtgcg ccgggcgctc gcccatgcca tcgaccgcaa ggcggtgatc gagggcgcga
     1201 tgaacggcgt tggggtgcca atcgggtcgc atttctcgcc ggcgcatccg gcctatgtcg
     1261 atctgaccgg cgcggtgccc tacgatccgg ccaaggccaa ggccctgctg cgggaagccg
     1321 ggttcccgaa cgggttttca acgacgctcc gtctgccgcc accgccctac gcccgccgct
     1381 ccggcgaggt gatcgccgcc atgctcggcg aggtgggcat caaggtctcg atagagccgg
     1441 tggaattccc gcaatggctg gagcgcgtgt tccgcaacaa ggaatacgac ctgtccatca
     1501 tcgcgcatac cgagccgctg gatgtgggga actacgcccg aaccgactac tactggggtt
     1561 acaacaacgc cgacttccag gcgatgaaca ccagggccaa cgccgagatg gacgaggcca
     1621 agcgcttcca gatctatcgc gacatgcaga agaagatcct cgacgatcag gtcaacgtgt
     1681 tcctgttcat gctgccgaag accacggtcg ccaagaagga cctagaaggc atgtggcaga
     1741 actggccgat cccggtctcg ccggcatccg aactgagctg gaaataggca cgggaatggg
     1801 gggaagacgt agcttttttg ctgcgttccg cgccatgagg ccagcgccat tgcctcgcat
     1861 cgcgtcccgt cgatgctgag cttcattctg cgccgtgtcg ccgccctggc gctgacgctc
     1921 tgcgtcacgg cggcggtgat cttcatcgtg ctggaaatcc tgccgggcga tccggccgcg
     1981 gtgatgatgg gcgtcaacgc gacgccggag aatgtcgcgg cgatccgggc agagtacggc
     2041 ctcgacaggc cggcggtgga gcggttcctc acctggatcg gcgggctgct gaccggcgat
     2101 ctcggcctca gctacaccta ccgcgtgccg atttcatcgc tgatcgccga gcgggcgcag
     2161 gtgacgctgc cgctggcggc gatggcggtg atgctctccg ccagcatcgg cattccgctg
     2221 ggaacgctgg ccgccgccaa ggccaacacg cgcacggatt cgctggtgat gggcatcagc
     2281 cagctgggcc tcgccgtgcc gaacttctgg ttcggcatcc tgctggtgct gctgttcgcg
     2341 gtgacactgg gctggtttcc ggccgggggc tttcccggct ggggggcggg ggtcctggcc
     2401 ggcatcaaga cgctgattct gcccgccgtg gcgctggcct tgccgcaatc cgcgatcctg
     2461 gcgcgtctga cgcgctcggc cgtgctggag gcgttgcagg aggatttcgt gcgcaccgcg
     2521 cgcgccaagg ggctgagctg gccggcgacc ctgcgaaagc atgtgctgcg caacgcgctg
     2581 gtgccggtgg tgacgatcct cggcctgcag ttttccgtgc tggtggcggg cgccatcatc
     2641 atcgagaacg tgttttcgct gcccggcctg gggcggctga tgttccagtc catcgcgcag
     2701 catgacatca tcgtggtcaa ggacctggtc gtgctgttcg cggcgctggt ggtgttcatg
     2761 aacttcatcg tcgatgtcgc ctacggcatc atcgatccaa ggctgaggcg gacgtgaatg
     2821 tcggggcccg agaccatcgc cgccgcgccg tcattccgcc gctcgccgtg gcggcatccc
     2881 tcgctgatca tcgggggaat catcgtcgcg ctgatcgtga cggcggcgct ggtgtcgctg
     2941 gtgtggacgc cgcatccgcc gacgaagctg aatatcccgt cgcggttgca ggggccaagc
     3001 gcgacgttct ggctcggcac cgaccacttc ggccgcgacg tgttctccat gctgatggtg
     3061 ggcgcacgca attcgctggc ggtgggcatc gtcgcggtat cgatcggcgc ggtctttggc
     3121 gtgctgctgg gcacggcctc ggcggcgcgc tcgggatcgt ggctggacga ggcgatcatg
     3181 cgcacggcgg acttcacctt cgccttcccg gcggtgctgt cggcggtgat gatcacggcg
     3241 ctggcgggac cgggggtcgt caacgccatc atcgccatcg gcatctacaa catcccgatc
     3301 ttcgcccggc tgacgcgctc cggggcgttg caggtctggt cgcgcgacta tgtgatggcg
     3361 gcgcgggcgg cgggcaagac cgcggcgcag atcacgatgc ggcacgtcat tcccaacatc
     3421 gccgggctga tcatcgtgca ggcgacgatc cagttcgcga ccgcgatcct ggcggaagcc
     3481 ggcttgtcct acctgggcct cggcacgcaa ccgccgaacc caagctgggg gcgcatgctg
     3541 ttcgaggcgc agacgaccat gcagcgccag ccgctgctgg cgatcttccc ggggctgacc
     3601 atcgttgtcg ccgtgctggg ctgcaacctc ttgggcgacg gcctgcgcga tgtgctcgac
     3661 ccgcgcgtca accggagccg gtcgtgaccc ccgcacatcg ggccgcacca tgcttctgag
     3721 cgtggacacc ctcacgatcg gcttcggccg caaggacgcg ccgatccgcg tggtgcggga
     3781 cctgtcgttc gcgctcgaaa aaggccagac gctcggcatc gtcggggagt ccggctccgg
     3841 caagaccatc acggcgctgt ccctgatggg cttgcaggcg agcgcagcgc atgtctccgg
     3901 ctcgatccgc ttcgagggcg aggaactgct gccgcatgcg gaagagcgga tgacggacat
     3961 ccgcggcaag tccatcgcca tgatcttcca ggagccaatg acggcgctca acccggtgca
     4021 tcggatcggc gaccagatcg ccgaggggct gatcctgcac gaagggttct cgcaggcggc
     4081 ggggctggcg gaggccgtgc gcctgctcga acgcgtcggc atcgcgcagg cgcaggagcg
     4141 catcgcggcc tatccgcatg aactctcggg cggccagcgc cagcgcgtga tgatcgccat
     4201 ggcattggcc tgccggcccg aactgctgat cgcggacgaa cccacctcgg cgctggacgt
     4261 gacggtgcag gcgcagctgc tcgacctcat ccgcgatgtg gtcgcggagc ggggcatggc
     4321 gctgatcgtg atctcgcacg accttgggat cgtcaccgag ctcgcccagc gcaccatcgt
     4381 gctctacggc ggcgcggtga tggaggaggg ggagacggt
//
LOCUS       DAOMXN010000210         4387 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_10747_length_4387_cov_10.830332, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000210 DAOMXN010000000
VERSION     DAOMXN010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4387
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10747_length_4387_cov_10.830332"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(253..924)
                     /locus_tag="PK812_12075"
     CDS             complement(253..924)
                     /locus_tag="PK812_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007196214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HRJ70331.1"
                     /translation="MTARADILANIRRSLGVAASERMRVQAVEDRIERSPRGVIPARG
                     QVDPVARRDLFKAQAEGVQATVEVVANAKDVPEAVARYLRERNLPATARIGADPVLVA
                     MPWEDTAIEVAHGKSEGRDLNAVSRAVAGVAETGTLVLHSGGDNPTTLNFLPDNHIVV
                     LKAGEIVGDYESVWSKLRMTFGKGELPRTVNFITGPSRSADIEQKLQLGAHGPRNLHI
                     VIVEK"
     gene            complement(921..2348)
                     /locus_tag="PK812_12080"
     CDS             complement(921..2348)
                     /locus_tag="PK812_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020180003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HRJ70332.1"
                     /translation="MSVHPTSNQFKGNVHQALGNAKLQQAMGFVRTGFIDKRKAAADR
                     LPEFEALRDNAREIKDHVLAHLDLYLERYEKKATEAGGQVHYARDAAEARNLILDICR
                     RVEAKTVTKGKSMIAEEIGLNAHLEAAGIEPIETDLGEYIIQLRGEIPSHIIAPAVHV
                     TQDQVESDFRRVHTHLDPARDLSEPSSLLAEARGVLRERFLKADVGITGANFLIAETG
                     TSIIVTNEGNGDLTQILPRVHIVLASIEKIVPTLEDVSQILRVLARSATGQEMSVYTT
                     FSTGPRRKGDPDGPEEYHVVLLDNGRSAMLGGEFEEMLRCIRCGACMNHCPVYHAVGG
                     HAYGWVYPGPMGAVLTPSLIGVDKAGHLPNASTFCGRCESVCPMRIPLPKMMRSWRER
                     EFERNLAPATVRSGLGLWAFFARRPRLYGLATNLAMKALTWAGRKKGRFTWLPLAGGW
                     TQYRDFAAPQGETFQAKWKRERGSK"
     gene            complement(2407..2910)
                     /locus_tag="PK812_12085"
     CDS             complement(2407..2910)
                     /locus_tag="PK812_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014131622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRJ70333.1"
                     /translation="MITPDYVRTMARYNRWQNGSLYSAAGTVSEAARREGRGAFFGSI
                     HGTLSHLFWGDSIWFSRFADWPKPSGGIKDSPAFRPDWAQLSAERETLDAAIIAWADA
                     LDPDWLTHDLVWFSGALGRELRRPRALLVVHFFNHQTHHRGQVHAMLTAAGAKPDDTD
                     LMIMPGQ"
     gene            complement(2920..4104)
                     /locus_tag="PK812_12090"
     CDS             complement(2920..4104)
                     /locus_tag="PK812_12090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRJ70334.1"
                     /translation="MTAIYDVVIAGGAVMGSSIAYHLAADPAFSGRILVLEKDRTYAR
                     AASALSASSIRQQFSQALNIRISLHGIRFLRDIAAHLSVEGEAPAIGLKEGGYLYVAG
                     DNGYRVLQDNHEVQRAEGADIALLDAVGLSARFPWLATADLAGGSYGVSGEGWFDGYG
                     LMQAFARKARSLGVEYRQAEVVEVERAGDRIAAVRLADGARIGCGVFVNACGASGARR
                     IALACGVPIPVYAKKRSVFSFTARERLERFPLLIDTSGVWCRPEGEGYICGVSPDDLD
                     ENDHGADFDVDWSQWDEIVWPALAARVPAFEAVRSGRAWAGHYDMNLFDHNAVLGRLG
                     DLTNAYIAAGFSGHGIQQSPAVGRGLAELIAHARYRTLDMSELAFGRIAAGRPLLEKN
                     VI"
     gene            complement(4174..>4387)
                     /locus_tag="PK812_12095"
     CDS             complement(4174..>4387)
                     /locus_tag="PK812_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70335.1"
                     /translation="AVLACIPMSRLMAALVWPALVSRTWHNVSPVMGERAGRPDAAKA
                     ESGTPSLARSVRVSAERLEDMGTIAA"
BASE COUNT          723 a         1479 c         1484 g          701 t
ORIGIN      
        1 cggtcgcgcc cggagccggc gcggcggccg gcggctgcgc cctcaacggc ttttcatcgc
       61 cgaacccctg catgggaacg gtctgggcca cggccggcga cagcccgaga gccagcgaag
      121 acacggcgaa cgccaacgca acgatacgca tcaacccctc gaagacgcta gggggcggcg
      181 atgcgccccg gattgcgcca agcatagggc gttagggtta ccgaaccctg acgaagcggg
      241 cgtcgcgccg gactacttct ccacgatcac gatgtggagg ttgcgcggcc cgtgcgcgcc
      301 gagctgcaac ttctgctcga tgtcggcgga gcgcgagggt ccggtgatga aattcaccgt
      361 gcgcggcaac tcgcccttgc cgaaggtcat gcgcagcttc gaccagacgc tctcataatc
      421 gccgacgatc tcgcccgcct tcagcacgac gatgtggttg tcggggagga aattcagcgt
      481 cgtcgggttg tcgccgccgg aatgcagcac cagcgtgccg gtctcggcga cgccagcgac
      541 ggcgcggctc accgcattga ggtcgcgtcc ctcgctcttg ccatgcgcga cttcgatggc
      601 ggtatcctcc cacggcatgg cgacgaggac agggtccgca ccgatgcgcg cggtcgccgg
      661 caggttgcgt tcacgcaggt agcgggcgac ggcctcgggc acgtccttgg cattcgcgac
      721 cacctcgacc gtggcctgca cgccttccgc ctgcgccttg aacaggtcgc ggcgggctac
      781 gggatcgact tgcccgcgcg ccggtatgac accgcgcggc gagcgctcga tccggtcctc
      841 gaccgcctgc acgcgcatgc gctcggaggc ggcaacgccg agcgaacggc ggatgttggc
      901 gagaatgtca gcgcgggcgg tcatttcgat cccctctccc gcttccattt cgcctggaac
      961 gtctcgccct gcggcgcggc gaaatcgcgg tattgcgtcc agccgccggc gagcggcagc
     1021 cacgtgaaac ggcccttctt gcggccggcc caggtcagcg ccttcatggc gaggttggtc
     1081 gccagcccgt agagcctcgg ccgacgggca aaaaaggccc agagaccgag accggagcgg
     1141 acggtggccg gcgccaggtt gcgctcgaac tcgcgctcgc gccaggagcg catcatcttc
     1201 ggcagcggga tgcgcatggg gcagacgctt tcgcagcgtc cgcagaaggt ggaggcgttg
     1261 ggcaggtgac ccgccttgtc gacgccgatc agcgagggcg tgagcaccgc acccatcggg
     1321 ccgggataga cccagccata agcgtggccg ccgaccgcgt gatagaccgg gcagtggttc
     1381 atgcaggcgc cgcagcggat gcagcgcagc atctcctcga actcgccgcc gagcatggcc
     1441 gagcggccgt tgtccaggag cacgacgtgg tattcctccg gcccgtccgg atcgcccttg
     1501 cggcgcgggc cggtggagaa ggtggtgtag acactcatct cttgtccggt ggcggagcgc
     1561 gccagcacgc gcaggatctg gctgacgtcc tccagcgtcg gcacgatctt ctcgatcgag
     1621 gcgagaacga tatgcacgcg cggcaggatc tgcgtcaggt cgccgttgcc ctcgttggtg
     1681 acgatgatcg aggtgccggt ctcggcgatg aggaagttcg cgccggtgat gccgacatcg
     1741 gccttgagga agcgctcgcg caacacgcca cgcgcctcgg cgagcaggct tgagggctcg
     1801 gacaggtcgc gggcggggtc gagatgggtg tggacgcggc gaaagtcgct ctccacctgg
     1861 tcctgcgtca catgcaccgc cggggcgatg atgtgagagg gaatctcccc cctgagctgg
     1921 atgatgtatt cgccgaggtc ggtctcgatc ggctcgatgc cggcggcctc caggtgggcg
     1981 ttgaggccga tctcctcggc gatcatcgac ttgcccttgg tgacggtctt cgcctccacc
     2041 ctgcggcaga tgtcgaggat gaggttgcgc gcctcggcgg cgtcgcgggc gtagtgcact
     2101 tgtccgccgg cctcggtcgc cttcttctcg tagcgttcga gatagaggtc gagatgggcc
     2161 agcacatggt ccttgatctc gcgggcgttg tcgcgcagcg cctcgaactc cggcaggcgg
     2221 tcggccgccg ccttgcgctt gtcgatgaag ccggtgcgca cgaagcccat ggcctgctgc
     2281 aacttggcgt tgccgagcgc ctggtggacg ttgcccttga actggttcga tgtgggatgg
     2341 acgctcatct gcccccgcct gtcattgccc cctgttctag cgactcgcag acggactcgc
     2401 cagcgcctac tggcctggca tgatcatcag gtcggtgtcg tccggcttcg ccccggccgc
     2461 cgtgagcatg gcgtggacct gcccgcgatg atgcgtctgg tgattgaaga aatgcaccac
     2521 cagcagcgcg cgggggcgac gcagttcccg gccgagcgcg ccggagaacc agacgagatc
     2581 gtgcgtgagc cagtccggat cgagcgcatc ggcccaggcg atgatggcgg catcgagcgt
     2641 ttcgcgctcg gcggaaagct gtgcccagtc cggccggaag gcgggcgagt ccttgatgcc
     2701 gcccgacggc ttcggccaat cggcaaaccg gctgaaccag atggaatcac cccagaacag
     2761 atggctgagc gtgccgtgga tggagccgaa gaaggctccc cggccctcgc gtcgggcggc
     2821 ttcggaaacg gtcccggccg cactgtagag gctgccattc tgccagcggt tgtagcgggc
     2881 catggtgcgg acatagtctg gcgtgatcat cggaccctct caaatgacgt tcttttccag
     2941 cagcggccgg ccggcggcga tgcgcccgaa ggcgagttcg gacatgtcga gcgtgcgata
     3001 gcgcgcgtgc gcgatcaatt cggccagtcc gcgcccaacc gcaggcgact gctggattcc
     3061 gtggccggag aagcccgcgg cgatataggc gttcgtcagg tccccgagcc ggccgagcac
     3121 cgcgttgtgg tcgaacagat tcatgtcgta gtggccggcc caggcgcggc cggatcgcac
     3181 cgcctcgaag gccgggaccc gcgccgcgag cgccggccag acgatctcgt cccactgcga
     3241 ccagtcgacg tcgaaatccg cgccgtggtc gttttcatcg agatcgtccg gcgatacgcc
     3301 gcagatgtag ccctcaccct ccggacggca ccagacgccg gacgtgtcga tcagcagggg
     3361 gaaacgctcc agccgctccc gtgccgtgaa ggagaacacg ctgcgcttct tggcatagac
     3421 cgggatcggc accccgcagg ccagcgcgat ccgccgcgcg cccgaggcac cgcaggcgtt
     3481 gacgaagacg ccgcagccga tgcgggcgcc atccgccaaa cgcaccgcgg caatgcgatc
     3541 gcccgcgcgc tcgacctcca cgacttccgc ctggcggtat tcgacaccca gagagcgcgc
     3601 tttccgcgcg aaggcctgca tcagcccata gccgtcgaac cagccctcgc cgctgacgcc
     3661 gtaactgccg ccggcgagat cggcggtcgc cagccagggg aagcgcgccg acaggccgac
     3721 ggcgtccagg agggcaatgt ccgccccctc cgcccgctgc acctcatggt tgtcctgaag
     3781 cacgcggtag ccattgtcgc ccgccacata gagatagccg ccctccttca gaccgatggc
     3841 cggcgcctcg ccctcgacgg acaggtgcgc cgcgatgtcg cgcaggaagc ggatgccatg
     3901 cagcgagatg cgaatgttca gggcttgcga aaattgctgg cggatggagc ttgcggacag
     3961 ggcagaggcg gcgcgggcat aggtccggtc cttttccaga accaggattc ggccggaaaa
     4021 cgccgggtcc gcagccagat gataggcaat ggacgacccc atcaccgcac cgccagcgat
     4081 caccacatca tagattgccg tcatgccgcc tgctttcgag ccggagcgct tggagcgcac
     4141 acgagttcac gctatcgatt ctgcatccag aacttaagca gcgatcgtcc ccatgtcttc
     4201 gagcctttcc gcagaaaccc gcacgcttct cgccaggctt ggcgtgccgg attccgcctt
     4261 cgccgcatcc ggtctcccgg cacgctcgcc catcaccggt gaaacgttgt gccacgtgcg
     4321 cgagacgagc gccggccaga ccagcgccgc catcaaacgg ctcatcggga tgcaggccag
     4381 gaccgca
//
LOCUS       DAOMXN010000211         4359 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_10839_length_4359_cov_11.481413, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000211 DAOMXN010000000
VERSION     DAOMXN010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4359
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10839_length_4359_cov_11.481413"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2479
                     /gene="dnaE"
                     /locus_tag="PK812_12100"
     CDS             <1..2479
                     /gene="dnaE"
                     /locus_tag="PK812_12100"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRJ70336.1"
                     /translation="PAPGLDEAAYRARLDFELNVIERMKYPGYFLIVADFIQWAKGQA
                     IPVGPGRGSGAGSLVAYALTITDLDPLRFGLLFERFLNPDRVSMPDFDIDFCQDRRDE
                     VITYVRQRYGNDRVAQIITFGTLLARGVMRDVGRVLGLPYTQVDRLTKLVPQNPAKPV
                     TLAEAVRDEAQLRAAAAENEGVARMLEIAQKLEGLYRHASTHAAGIVIADRPLEELVP
                     LYRDPKSDMPVTQFNMKWVEPAGLVKFDFLGLKTLTVLQGAVDLLAQRGVAVDLSNIP
                     LDDAATYALLSRGDTVGVFQVESAGMRRALLDMKPDRLEDIIALVALYRPGPMANIPV
                     YCEVKHGLREPDYYHPRLEPILKETFGVIVYQEQVMQIAQVLSGYSLGEADLLRRAMG
                     KKIKAEMDAQRDRFVRGSVERGEKQADADMIFDLLAKFADYGFNKSHAAAYALVSYQT
                     AYLKANYPVEFLAASMSLELTNTDKLAEFRREAARLEITVEPPDVNTSGVRFDVADGR
                     IRYALAAVKGVGEEAGRALVEARGARAFASVADFSARVSPKAVNKRTFEQLVAAGAFD
                     RLEKSRARLIAGIDRIFAHAAATEHEAQSGQTRLFGGGGQDEALQLPEADAWPLTERL
                     KREHAAVGFYLSGHPLDDYAEALRRLAVPDFAVFARSVKAGASHGRLAVSVLDRQERR
                     TKTGNKMGIVTLSDRSAQFEAILFAEGLAQLRPLLEPGAVLLLSVAASMEGEEVRLRI
                     QDAEPLDRAVTRVQKAVRVFVREDAPVAAIGQRLSRRGEGAVSLVILLDGAEGEVEVR
                     LPGQYDVSPAAVGAMKSLAGVIDVRAI"
     gene            2661..3662
                     /locus_tag="PK812_12105"
     CDS             2661..3662
                     /locus_tag="PK812_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRJ70337.1"
                     /translation="MALPDVSMRQLLEAGAHFGHQSHRWNPKMAPFIFGVRNKIHIID
                     LSQSVPMLHRALQAVSDTVARGGRVLFVGTKRQAQDAIAENAKRCAQYYVNSRWLGGM
                     LTNWKTISASIQRLRKVEETLAGGAHGLTKKERLMLSRERDKLDRALGGIKDMGGTPD
                     LIFIIDTNKEQLAIQEARRLNIPVVAVVDTNCDPDGITYAVPGNDDAGRAIALYCELI
                     ARAAIDGIGRGQGDAGIDLGSAAEPVVEPLPAAAGAEGEAAFERLSAPRGAPDDLTKL
                     GGLGPELEKKLNEAGIFHFWQVAAMGADDEKKLDKDLKLHGRIGREGWVAKARELVV"
     gene            3774..>4359
                     /gene="tsf"
                     /locus_tag="PK812_12110"
     CDS             3774..>4359
                     /gene="tsf"
                     /locus_tag="PK812_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HRJ70338.1"
                     /translation="MAEITAALVKQLREMTGAGMMDCKAALNETKGDIEAAVDWLRKK
                     GLSKAAKKSGRVAAEGLVAMSVAGGKGVVVELNSETDFVARNEQFQALARTIAGIAAK
                     GAGDVEALKVAAYPGGGTVTEAISNAVATIGENMTLRRTASLAVGQGVVASYMHGAVA
                     DGLGKIAVLVALESSAPADALAGIGRQVAMHVAAT"
BASE COUNT          721 a         1320 c         1553 g          765 t
ORIGIN      
        1 accggccccg ggactcgacg aggcggccta tcgcgcgcgg ctcgatttcg aactgaacgt
       61 catcgagcgc atgaaatacc caggatactt cctgatcgtg gcggatttca tccagtgggc
      121 caaggggcag gccattccgg ttggtcctgg gcgcgggtcg ggcgccgggt cgctggttgc
      181 ctatgcgctg accatcactg accttgatcc gctgcgcttc ggcctcctgt tcgagcggtt
      241 tctcaatccg gaccgcgtct cgatgccgga tttcgatatc gacttctgcc aggatcggcg
      301 cgacgaggtc atcacctatg tgcgccagcg ctacggcaat gaccgcgtgg cgcagatcat
      361 caccttcggc acgctgctgg cccgcggcgt gatgcgggat gtcgggcgcg tgctcggcct
      421 gccctacacg caggtcgacc ggctgaccaa gctggtgccg cagaaccctg ccaagcccgt
      481 gacgctggcc gaggcggtgc gtgacgaggc gcagttgcgc gcggcggcgg ccgagaacga
      541 gggcgtcgcg cgcatgctgg agatcgccca gaagctggag gggctctatc ggcacgcctc
      601 gacccatgcc gccggcatcg tcatcgccga ccggccgctg gaagagctgg tgccgcttta
      661 ccgcgacccg aagtccgaca tgccggtgac ccagttcaac atgaagtggg tggagccggc
      721 ggggctggtg aagttcgact tcctcgggct gaagacgctg accgtgctgc agggcgcggt
      781 cgaccttttg gcgcagcgcg gcgtggcggt cgatctgtcg aacatcccgc tggatgatgc
      841 ggcgacctat gccctgctgt cgcgcggcga cacggtcggg gtgttccagg tggaaagcgc
      901 tggcatgcgc cgggcgcttc tcgacatgaa gcctgatcgt ctggaggaca tcatcgcgct
      961 ggtggcgctc taccggccag gaccgatggc taatattccg gtctactgcg aggtgaaaca
     1021 tgggctccgg gagccggatt attaccaccc caggcttgag ccgatcctga aagagacctt
     1081 cggcgtcatc gtctatcagg aacaggtgat gcagatcgcg caggtcctgt ccggctattc
     1141 gctgggcgag gcagatcttt tgcgccgggc gatgggcaag aagatcaagg cggaaatgga
     1201 tgcgcagcgc gaccggttcg tgcgcggctc cgtggagcgt ggcgagaagc aggccgacgc
     1261 ggacatgatc ttcgatctgc tggccaagtt cgccgactat ggcttcaaca agagccatgc
     1321 cgcagcctac gcgctcgtgt cgtatcagac ggcgtatctg aaggcgaatt acccggttga
     1381 gttcctggcc gcctcgatga gcctcgaatt gacgaatacg gacaagctcg cggagttccg
     1441 gcgcgaggcg gcgcgtctgg agatcacggt ggagccgccg gacgtcaaca cctcgggcgt
     1501 gcgtttcgat gtggccgacg ggcgcattcg ctacgccctg gcggcggtga agggcgtcgg
     1561 cgaggaggca gggcgcgcgt tggtggaggc gcgcggcgcc agggctttcg cgtctgtcgc
     1621 ggatttttcc gcacgggtca gccccaaggc cgtcaacaag cggaccttcg aacaattggt
     1681 cgcggccggc gccttcgacc ggctggagaa gagcagggcg cggctgattg cgggcattga
     1741 caggatcttt gcccatgccg ccgcgacgga gcacgaggcg cagagcgggc agacccgatt
     1801 gtttggcggc ggcggccagg acgaagctct gcagttgccc gaggccgatg cctggccgct
     1861 gacggagcgc ctgaagcgag aacatgccgc cgtcggtttt tacctttccg gtcacccgct
     1921 ggacgattac gccgaggccc tgcggcgtct tgcggtgcct gacttcgcgg ttttcgcgcg
     1981 gtccgtgaag gccggcgcct cgcatggccg gctggccgtg agcgttctgg atcggcagga
     2041 acggcgcacg aagaccggca acaagatggg gattgtcacg ctttctgatc gttcggcaca
     2101 gttcgaggcg atcctttttg ccgaagggct ggcgcagttg cgtcccctgc ttgagccggg
     2161 cgccgtgctg ctcctgtcgg tcgccgcctc gatggagggc gaagaggtgc gtctgcgcat
     2221 ccaggatgcg gagccgctcg accgggcggt gacccgcgtg cagaaggcgg tccgggtctt
     2281 tgtccgggag gatgcgccgg tggccgccat cggtcagcgg ctgagccggc gcggcgaggg
     2341 ggcggtatcg cttgtcattc tgctcgacgg tgccgagggc gaggtcgagg tccgtctgcc
     2401 ggggcagtat gacgtgagcc cggcggcggt cggcgccatg aagtcgctgg ccggagtcat
     2461 tgacgtgcgg gccatctagg gattgaggcc gccgcttttc cggtatttgg tcctgtgcag
     2521 cctcttgtcc tgcgctgcct cttgcctttt cgggcaaaac cggccatagg cgcgccattc
     2581 cacatgcagg gctggttttc gaaccgtccg gtgccggatc cccggctccg ctctgcagag
     2641 gcattaaccg gagaagactg atggcgttgc ccgacgtttc gatgcggcag ctcttggaag
     2701 ctggcgcgca ttttggacac caatcccacc gctggaaccc gaaaatggct ccgttcattt
     2761 tcggcgtgcg gaacaagatt cacatcatcg acctgtcgca gtccgtgccg atgctgcacc
     2821 gtgcgctgca ggcggtgagc gacaccgtgg cgcggggcgg tcgcgtgttg ttcgtcggca
     2881 ccaagcggca ggcgcaggac gcgattgccg agaacgccaa gcgttgcgcg cagtactacg
     2941 tgaattcccg ctggctcggc ggcatgctga ccaactggaa gacgatttcg gcgtcgatcc
     3001 agcgcctgcg caaggtcgag gagaccttgg ctgggggcgc gcatggcctc accaagaagg
     3061 agcgcctgat gctctcgcgc gagcgcgaca agctcgatcg cgcgcttggc ggcatcaagg
     3121 acatgggcgg cacgccggac ctcatcttca tcatcgacac caacaaggaa cagctggcga
     3181 ttcaggaagc gcgccggctc aacattcctg tggtggcggt ggtcgacaca aactgcgatc
     3241 cggacggcat cacctatgcc gtgcccggca acgatgacgc gggacgggcg atcgcgctct
     3301 actgtgagtt gattgcccgc gccgccattg atggcatcgg tcgtggccag ggcgatgcgg
     3361 ggatcgatct gggatcggcg gcggaacccg tcgtcgagcc gctcccggcg gctgcgggtg
     3421 ctgaagggga agcggctttc gagcgtctgt ccgctccgcg cggcgctccc gacgacctga
     3481 cgaagcttgg cggtctcggc ccggaactgg agaagaagct gaacgaagcc ggcatcttcc
     3541 acttctggca ggtcgcggcc atgggcgcgg atgacgagaa gaagctcgac aaggacctca
     3601 agctgcatgg ccgcatcggc cgcgagggct gggtcgccaa ggcgcgcgaa ctggtggtgt
     3661 gacgccgcca cgatgcccgg cctgtcgccg ggatcccgag tgatcgcggc ccggaacgca
     3721 gcaacgttcc gggtttgcgt ttgagattgc gattgtctga aaggaacgga gctatggcgg
     3781 agattaccgc ggcgcttgtg aaacaactgc gcgaaatgac cggcgccggc atgatggact
     3841 gcaaggccgc tctgaacgag acgaagggcg acatcgaggc tgcagtcgac tggctgcgca
     3901 agaaagggct gtcgaaggcc gccaagaagt cgggccgcgt cgcggccgag ggcctggtgg
     3961 cgatgtcggt cgcgggcggc aagggcgtgg tcgtcgagtt gaattccgaa accgacttcg
     4021 tggcccgcaa cgagcagttc caggcgctgg cgaggaccat cgccgggatt gccgccaagg
     4081 gcgccggcga tgtcgaggcg ctgaaggtcg cggcctatcc gggtggcgga acagtgaccg
     4141 aggcgatttc caatgccgtt gccacaatcg gcgagaacat gaccctgcgg cgcacggcca
     4201 gcctcgccgt cgggcagggg gtcgtcgcca gctacatgca cggcgccgtc gccgacggcc
     4261 tcggcaagat cgcggtgctg gtggctctcg agtcgagcgc gccggcggat gcgcttgccg
     4321 gcatcggccg tcaggtcgcg atgcatgtag ccgcaacca
//
LOCUS       DAOMXN010000212         4329 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_10935_length_4329_cov_10.620964, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000212 DAOMXN010000000
VERSION     DAOMXN010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10935_length_4329_cov_10.620964"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="PK812_12115"
     CDS             <1..652
                     /locus_tag="PK812_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRJ70339.1"
                     /translation="ARLGSLAFCAAEAPGEAVARAHPDASLIAFRHGERVCVFAVEQA
                     LRSDAGETVRALKTAGYAVEILSGDRMEAVRRAARTLGIDEVHGELKPSEKIARLHAL
                     AGMGRKVLMVGDGLNDAPSLAAAHVSLSPITAVHLAQAAADGVFLGDALRPVSESVRV
                     AKRARKVMAENLWLAVVYNFIAVPLAIAGLVTPLIAALAMSGSSVIVTLNALRAKG"
     gene            662..811
                     /gene="ccoS"
                     /locus_tag="PK812_12120"
     CDS             662..811
                     /gene="ccoS"
                     /locus_tag="PK812_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HRJ70340.1"
                     /translation="MNILVILVPLALLLGLSGLAAFMWSLNRKQFDDLEGAGWRAISD
                     DDIGR"
     gene            906..1634
                     /gene="pxpB"
                     /locus_tag="PK812_12125"
     CDS             906..1634
                     /gene="pxpB"
                     /locus_tag="PK812_12125"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HRJ70341.1"
                     /translation="MDRTDYPRVRPCGDCALTIELGNEIDATRNARVLSLDAAVAAAR
                     KAGVIETIPTYRSLFVAYDPGLIGYAPLAAWLLDLAGGALPETGSGRSWRFPVTYGGA
                     FGEDMPFVAQKSGLSEAEVARRHAGGTYRIYMMGFMPGFTYLGGLDPALALSRRDNPR
                     LTSPAGTISIGGVQTGIQCLVSPTGWHLIGRTPARTYDPQRSRVFFLEPGDSVSFFPV
                     GPEDFAALERRAEAGELVAEPVAP"
     gene            1631..2650
                     /locus_tag="PK812_12130"
     CDS             1631..2650
                     /locus_tag="PK812_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HRJ70342.1"
                     /translation="MSVIEVVEAGPYTSVQDTGRYGAQRYGLGTAGAMDTLSLAIANV
                     LVSQPAGAAAIEIGPLPARFRITDGAVRLALAGGVRGATVQGRALPMGETVLLAEGDV
                     LALRACSQGVFSYLAFEGGIDGEPVFGSFSVHARSGIGSPYSRALLPGDRLNVGGAAV
                     RGAERSLAAPTRGSGALRVVLGPQDDHFEPETIAQFLDTEWKISATSDRMGYRLEGPA
                     LRHKGSPNILSDGIANGSVQIPGNGLPLLLLADRGTTGGYPKLATIISADLGRLAQVP
                     VGSVIRFQAIAVEEAQAEARAHAALISSLPRAVRDVGGGDLSSEFLLASNLAGAATSA
                     DDWSI"
     gene            2662..3429
                     /locus_tag="PK812_12135"
     CDS             2662..3429
                     /locus_tag="PK812_12135"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HRJ70343.1"
                     /translation="MRVDLNSDLGESFGPWPMGDDPAMLDLVSSANIACGFHGGDPQV
                     MLRACTLAKARDVSIGAHPGFLDLQGFGRRQIVGLSAEEVETFVAYQIGAIRGVAALA
                     GARVTHVKIHGAMSNMACEDEGMAAAIARAIRGVDPDLIFVVLPFSALQRAGEKAGLR
                     LAMEVFADRAYEDNGLLMSRKKPGSVIHDGAAAAANVLRMVRQQAVTTGSGKVMPTRV
                     DTICIHSDTPGAIAMARQVRQVLVDASVAIRPFASWL"
     gene            complement(3483..4115)
                     /locus_tag="PK812_12140"
     CDS             complement(3483..4115)
                     /locus_tag="PK812_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HRJ70344.1"
                     /translation="MIRATSVQAAGAWHGAPADTVVLDFDARTRGQTRMSGIGGQDFQ
                     LDLAQTVTLRTGDALVLEDGRLIEVVAKPESLIEIRAANETELARIAWRLGNGHTAVQ
                     VSGKKLRIRNDHAIVEMLRDLGAKLTEIEAPFDPEGGAYLTAPAACDHPDHAHGHACG
                     HHHHGHAHHDHDHHHGHHKHHDHDHKHGHEHGDECGCGHDHHHGKGHKHG"
BASE COUNT          649 a         1338 c         1527 g          815 t
ORIGIN      
        1 ggcgcggctg ggcagcctgg ccttctgcgc ggccgaagcg ccgggcgaag cggtcgcgcg
       61 ggcgcatccg gatgcgtcgt tgatcgcgtt ccggcatggt gaacgggtgt gcgtcttcgc
      121 cgtcgaacag gcgctgcgca gcgacgcggg cgaaacggtg agggcgctga agaccgctgg
      181 ctacgccgtc gaaatcctgt ccggagaccg gatggaagcc gtgcgccgcg ccgcgaggac
      241 ccttggcatc gacgaggtgc acggcgaact gaagccaagc gagaagattg cgcgcctgca
      301 tgccctcgcg ggcatggggc ggaaggtgct gatggttggc gacgggctga acgatgcgcc
      361 ttcgctggcg gcggcgcatg tttcgctgtc gccgatcacg gcggtacatc tggcgcaagc
      421 cgcggcggac ggtgtgttcc tgggcgatgc cttgaggcct gtgtccgaat cggtccgggt
      481 ggccaagcgt gcccgcaagg tcatggccga gaatctgtgg ctcgcggtgg tttacaactt
      541 cattgccgtg ccgctggcca tcgccgggct ggtcacgccg ctcatcgcgg cgctggcaat
      601 gtccggatct tccgtcatcg tcaccttgaa tgcgctgcgg gcgaagggat agggtgaagg
      661 catgaacatt ctggtcattc ttgttccgct tgccctgctt ctgggcctca gcgggcttgc
      721 cgctttcatg tggtccttga accgcaagca attcgacgat ctcgaagggg ccggctggcg
      781 tgccatcagc gacgacgata tcggccgctg aaggcactgt tccgtcgatt tgccggttgc
      841 cttggccgcg gcgttcatga tcctattctg ttcggcccgg gggagcgggt cgccggaagg
      901 caggaatgga caggaccgat tatccgcgtg tgcgcccctg cggtgattgc gcgctcacca
      961 tcgaactggg caatgaaatc gatgcgacgc ggaacgcacg tgtcctgtcg cttgatgccg
     1021 ctgtcgctgc cgcccgtaag gccggtgtca tcgagacgat cccgacctat cgctcgttgt
     1081 tcgtcgccta tgatcccggc ctgatcggct atgcaccgct ggcggcctgg cttctggatc
     1141 tggcgggagg agctttgccg gaaaccggga gcggacgaag ctggcggttt cccgtcacct
     1201 atggcggcgc cttcggcgag gacatgccgt tcgtggcgca gaaatccggc ctcagcgaag
     1261 cggaggtggc acggcgccat gccggaggga cgtaccgcat ctacatgatg gggttcatgc
     1321 ccggcttcac ctatctcggc gggctcgatc cggcgctggc cttgtcgcgc cgggacaatc
     1381 cgcgcctgac cagtccagcc ggcacgatct ccatcggggg cgttcagacc ggcattcagt
     1441 gtctggtctc gccgaccggc tggcatctga tcggccgcac gccggcgcgg acctacgatc
     1501 cgcagcgctc gcgggtgttc tttctcgaac ccggtgacag cgtgagcttt tttccggtcg
     1561 gcccagagga tttcgcggcg ctggagcggc gcgcggaagc gggtgaactc gtggccgagc
     1621 cggtggcgcc atgagcgtga tcgaggtcgt cgaggccggc ccatacacca gcgtgcagga
     1681 caccggccgc tatggcgccc aacgctacgg gctggggacg gcgggtgcga tggatacgct
     1741 gtcgctggcg attgcgaacg tgctggtgtc ccagcctgcc ggcgcggccg cgattgagat
     1801 cgggccgcta ccggcgcggt ttcgcattac ggatggcgcc gtccggctgg cgcttgccgg
     1861 cggcgtgcgc ggagcgacgg tgcaggggcg cgccttgccc atgggagaaa cggtgttgct
     1921 ggcggaaggc gacgtgctgg cgttgcgcgc ctgctcgcaa ggtgtgttca gctatctcgc
     1981 cttcgaaggc ggcattgacg gtgaaccggt cttcggcagt ttctcggtgc acgcccgatc
     2041 gggcatcggt agcccctatt cgcgagcgct gctgcccggt gacaggctga atgtcggcgg
     2101 cgcggcggtg cggggcgcgg aacgcagtct cgccgcgccg acccgcggca gcggcgcgct
     2161 gcgcgtcgta ctcgggccgc aggacgatca tttcgagccg gaaaccatcg cgcagttcct
     2221 ggatacggag tggaagatat ccgccacatc ggaccgcatg gggtatcggc tggaaggccc
     2281 cgctctccgg cacaagggca gcccgaacat cctgtccgac ggcattgcca atgggtcggt
     2341 gcagatcccg ggcaacggac tgcccctgct tctgctcgct gaccggggaa cgaccggggg
     2401 ataccccaaa ctcgcgacca tcatctccgc cgatctcggg cggctggcgc aggttccggt
     2461 cggttccgtg atccgttttc aggcgattgc cgtcgaggag gcgcaggccg aggcccgtgc
     2521 acatgccgct ctgatctcca gtctgccccg cgccgtccgc gacgtcggcg gcggcgacct
     2581 gtccagcgag ttcctgcttg cttcgaacct tgccggcgcc gccaccagcg cggatgactg
     2641 gagcatctga tgggagacgc catgcgtgtc gatctgaaca gtgacctcgg ggagagcttc
     2701 ggaccctggc cgatgggcga cgatccggcc atgctcgacc tggtatcctc ggcaaacatc
     2761 gcctgcggct tccatggtgg cgatccgcag gtgatgttgc gggcctgcac gctggcgaag
     2821 gcgcgggacg tgtccatcgg ggcgcatccg ggcttcctcg acctgcaggg gttcggccgg
     2881 cggcagatcg tcggactcag cgccgaggaa gtcgagacgt tcgtcgccta ccagatcggc
     2941 gccatccgcg gtgtcgccgc gctggcgggt gcccgggtta cgcatgtgaa gatccacggg
     3001 gccatgtcga acatggcctg cgaggacgag gggatggccg ccgccattgc ccgggccatc
     3061 cggggcgtcg atcccgatct gatcttcgtt gtcctgccgt tctccgcctt gcagcgtgcc
     3121 ggcgagaagg cggggctgcg gctggcgatg gaagtgttcg ccgaccgcgc ctatgaggac
     3181 aacggcctgc tgatgtcgcg caagaagccg ggctcggtca tccatgatgg cgcggcggcg
     3241 gctgcgaatg tgctgcggat ggtgcggcag caggccgtga ccaccggttc gggaaaggtc
     3301 atgccgacgc gcgtggatac gatctgcatc cactcggata cacccggagc catcgccatg
     3361 gcccggcagg tgcgtcaggt gcttgtcgac gcgagcgtgg cgatcaggcc gttcgcaagc
     3421 tggctatagc acgcggttcg gtccgattgg acctttcgcc aagcgccgca atcgcggacg
     3481 cgctagccgt gcttgtggcc tttcccatga tggtggtcat ggccgcagcc gcactcatcc
     3541 ccgtgttcat gcccatgctt atggtcatgg tcatggtgct tgtggtggcc atggtggtga
     3601 tcatgatcat gatgcgcgtg gccatgatga tgatgcccac aggcatggcc atgcgcgtgg
     3661 tcaggatggt cgcaggcggc cggagccgtc agataggcac cgccttcggg atcgaaaggt
     3721 gcctcgatct cggtgagctt ggcgccgagg tcgcgcagca tttctacgat ggcgtgatcg
     3781 tttcgaatcc gcagcttctt gcctgacacc tgcacggccg tatggccgtt gccgagacgc
     3841 caggcgatgc gtgccagttc ggtctcgttc gcggcgcgga tttcaatcag actttccggc
     3901 ttcgccacga cttcgatgag gcggccgtct tcgagaacaa gggcatcgcc cgtgcgcagg
     3961 gtgaccgtct gcgcaagatc gagctggaag tcctgcccgc cgatgccact catcctggtc
     4021 tgaccgcggg tgcgcgcgtc aaaatcgagg acgacggtgt cggcaggtgc gccgtgccag
     4081 gcgccggcgg cctgcacgga agtggcgcgg atcatgtgag cctcttggag ttccctgaac
     4141 ttcatcctga tcgctagcag atcgcgccgg gaacgccagc cccttgccgg cccgactaga
     4201 gcattgtccg atcagattga atcgttgcga tgcgtcgatt tggttcaagg ctggcgatgc
     4261 tgccgaagcg cgtgtgcgtg atacggtcga ggcggtgtcg cttggaattg ggccaaatcc
     4321 acgcatccc
//
LOCUS       DAOMXN010000213         4322 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_10955_length_4322_cov_9.725334, whole genome shotgun sequence.
ACCESSION   DAOMXN010000213 DAOMXN010000000
VERSION     DAOMXN010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10955_length_4322_cov_9.725334"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..998)
                     /locus_tag="PK812_12145"
     CDS             complement(12..998)
                     /locus_tag="PK812_12145"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRJ70345.1"
                     /translation="MTMPFSRRSVLAAGVGVLGAPALVRAQTTGARQTRIVVPFPPGN
                     ASDVAARLISTRLSEKLGQSFYVENKPGASGVIGIKEVIAAAPDGHTLLLTSLSPLVI
                     LPHLRKDTPYDPLKDLDPVALVGWTGMALVATPDFPASTLKDAVEHIRANPGKYNYGH
                     VGPGSLSHVSMVLLMRAAKLDVTAVSYRGSGEAMTEMMAGRLHLMFDGMTSSLPQVAG
                     GKVKGLAVNSPTRSTLAPDLPSLREVGIPVEHDVTGWTGLLAPRGTPAAIIDRLNAEV
                     NAIIQEKEFQEKSRILFLEAWKPESPAAFSAYMARELAKWGQALEAAGVKSQ"
     gene            1102..2307
                     /locus_tag="PK812_12150"
     CDS             1102..2307
                     /locus_tag="PK812_12150"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRJ70346.1"
                     /translation="MPLNLLEGVRILAVEQYGAGPFGTQMLADLGAEVIKIENPADGG
                     DYGRTVGPHFSPDVPKDRASYFFQGLNRNKKSLALDISTPAGRDVFLKLVAVSDAVTG
                     NLRGDVPDKLGIAYAQLKEANPRIVCAHLTGYGRHGERARWPGYDYLMQAEAGYFHLT
                     GDPEGAPSRMGLSLIDLMTGAVTGLALVSAVMKARATGQGQDVDVSLFDLALFNLNYI
                     GHWYLNAGASTTRLPRSAHPSLTPCQTYRTKDGWIYLMCNKEKFWPLLCARIGRPELA
                     ADARFATFPDRLRERETITALLDEALSVHTTAEWMAVFAGDIPAAPILDVAQALDNPY
                     VRENGGIDVIPLPGGGRLELLASPVRAEGQSPNSPAPGLGEHTESVLRAAGVDPADLA
                     ALRAQGIAR"
     gene            2304..3086
                     /locus_tag="PK812_12155"
     CDS             2304..3086
                     /locus_tag="PK812_12155"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRJ70347.1"
                     /translation="MTATQRDTAPLYARISQELGREISDGRYPQGALMPPELELCAKY
                     GVSRITMRAAMEELRRNGLISRRAGVGTRVEARTPRDVFVHESDSVESILHFTDQLEF
                     RLIETHRITAQRSAAEWLGCATGARLVLLRGLRVGQSGPPVALSAHHVPETDAAFARR
                     AHGQKGSIATRIEAETGRRIATIRQTIEPVALGAREASLLQAGEGDPALMTWRWHLDD
                     RDVLLLASNSLFPRGRYVWSQTVRRRIAPPELAPVLSAKEPR"
     gene            3083..4123
                     /locus_tag="PK812_12160"
     CDS             3083..4123
                     /locus_tag="PK812_12160"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HRJ70348.1"
                     /translation="MTVPSAYFRQSDGAFRERFGLSFEDFSVGQRFLHRPGITLSQQE
                     NVEESLDTLNAAMLHYDAHYAAQTAWKRPLLVSTITLKRAIGMGSKTLGRRRAILRFD
                     EIAMTAPLFGGDTIYSESEVLDIEPGDRSDCGTVRLRTSAVKADGIVAGRFVWLGAFW
                     RRGHGPDAEPGTPCETARFSAFRKLENGALMEEQGLFYEDFEEGDCFIHYPRRSFSEV
                     ELIEQTRRALDLSPFWQDPAQRSETGALRVPEPVLIGAATALTTRTFGRVVANLGWTD
                     TELPAPVFAGDTIEARSRIVARRDSRSRPGEGVLTVDTSVRNQRDVTVLSFRRNLLVY
                     RRSSAPYAQAGY"
     gene            complement(4126..>4322)
                     /locus_tag="PK812_12165"
     CDS             complement(4126..>4322)
                     /locus_tag="PK812_12165"
                     /inference="COORDINATES: protein motif:HMM:NF039218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SGNH hydrolase domain-containing protein"
                     /protein_id="HRJ70349.1"
                     /translation="DAFLRSLEGEGVHVIALRRLLCPQDRCSATLDGAFLYRDKAHLA
                     AEGSTRMGIRFPDFRLRERN"
BASE COUNT          735 a         1443 c         1432 g          712 t
ORIGIN      
        1 gatcgatccc gctactgcga cttgacgccg gcggcctcca gcgcctggcc ccatttcgcc
       61 agttccctgg ccatgtaggc agagaaggcc gcagggcttt ccggtttcca ggcctcaagg
      121 aaaaggatcc gcgatttttc ctggaattcc ttttcctgga tgatcgcgtt cacctcggca
      181 ttcagcctgt cgatgatggc cgcaggcgtg ccgcgcgggg ccagcaggcc ggtccacccc
      241 gtcacgtcgt gctccaccgg gatgccgacc tcgcgcaacg agggaagatc aggcgcaagc
      301 gtcgagcgcg tcggcgagtt cacggcaagg cccttgacct tgccgccggc cacctgcggc
      361 agcgacgagg tcatgccatc gaacatcaga tgcaggcggc cggccatcat ctcggtcatc
      421 gcctcgcccg agccgcgata ggaaacggcc gtgacgtcca gcttcgcggc gcgcatcagc
      481 agcaccatgg agacatggct cagcgacccg ggaccgacat gcccgtagtt gtacttgccg
      541 gggttggcgc ggatgtgctc gaccgcgtcc ttcaaggtgc tggcgggaaa atcgggtgtc
      601 gccaccagag ccatgccggt ccagcccacg agggcgactg gatcgagatc tttcaaagga
      661 tcgtaagggg tgtccttgcg caaatgcggc aggatcacca gcggcgacag cgaggtcagc
      721 agcagcgtgt gcccgtccgg cgcggccgcg atcacttcct tgatgccgat cacgccggaa
      781 gcgcccggct tgttctcgac atagaaggac tgcccaagct tttccgacag tcgcgtgctg
      841 atcaaccggg ccgccacgtc cgaggcgttg cccggcggaa agggcacaac gatgcgggtc
      901 tggcgcgcgc ccgtggtctg cgcccggaca agggccggcg cgcccagcac gccaacacct
      961 gcggccagca cggaccggcg actgaacggc attgtcatga ctccctcccg gacagtgaga
     1021 ttttcgcttt tcagcactga gccataatgt cataacaaag tccagcagga ttcctgaaga
     1081 tctaccgacc ggccgagcac catgcccctc aacctccttg aaggcgtccg catcctcgcg
     1141 gtcgagcagt acggcgccgg gcccttcggc acgcagatgc tggccgatct gggcgcggag
     1201 gtgatcaaga tcgagaatcc ggcggatggc ggcgactatg gccgcacggt cggaccgcac
     1261 ttcagcccgg acgtaccgaa ggacagggcg agctacttct ttcagggcct gaaccgcaac
     1321 aagaagagcc tcgcgctgga catttccacg ccggccggac gcgacgtgtt cctgaagctc
     1381 gtggcggtct cggatgccgt caccggaaac ctgcgcggcg acgtgccgga caagctgggc
     1441 attgcctatg cccagttgaa ggaggccaat ccgcgcatcg tctgcgcgca tctgaccggc
     1501 tacggcaggc atggcgagcg ggcgcgctgg ccgggatacg actacctgat gcaggccgag
     1561 gccggctact tccacctgac gggcgacccc gagggcgccc cctcgcggat gggcctgtcc
     1621 ctgatcgacc tgatgaccgg ggccgtgacc ggtctggcgc tggtctcggc ggtcatgaag
     1681 gcccgcgcga caggccaggg tcaggatgtg gacgtgtcgc tgttcgacct cgccctgttc
     1741 aacctgaact acattggcca ctggtatctc aacgccggtg cgtcgacgac ccggctgccc
     1801 cggtccgccc atccctcgct cacgccgtgc cagacctacc ggaccaagga cggttggatc
     1861 tacctgatgt gcaacaagga gaagttctgg ccgctgctgt gcgcgcgcat cggccggccg
     1921 gagctggcgg cggatgcgcg gttcgcgacg tttcctgacc gactgcggga acgggagacg
     1981 atcaccgcac tgctcgacga agccctgtcg gtgcacacga cggcggagtg gatggctgtc
     2041 tttgccggag acattccggc tgctccgatc ctcgatgtgg cacaggcgct ggacaacccc
     2101 tatgtgcgcg agaacggcgg gatcgacgtc attcccctgc ccggcggcgg gcggctggaa
     2161 cttctcgcca gcccggtgcg cgcggaaggc cagtcgccga acagtccggc tcccgggctg
     2221 ggcgagcaca ccgagagcgt gttgcgcgcg gcgggtgtgg acccggcaga cctggccgca
     2281 ttgcgcgccc aggggatcgc gcgatgacgg cgacacagcg cgataccgcc cccctctatg
     2341 cccgaatctc gcaggaactg ggccgggaga tcagcgacgg gcgctatccg cagggtgcgc
     2401 tgatgccgcc ggagctggag ctgtgcgcga agtacggcgt cagccgcatc accatgcgcg
     2461 ccgccatgga agaactgcgg cgcaacggcc tgatttcgcg ccgggcgggt gtcggcacgc
     2521 gggtggaggc acggacgccg cgcgatgtct tcgtccatga atccgacagc gtcgagagca
     2581 tcctgcactt caccgaccag cttgaattcc gcctgatcga gacgcatcgg ataaccgcac
     2641 agcgcagcgc ggcggaatgg ctgggctgtg ccacgggcgc gcgactggtg ctgctgcggg
     2701 gcctgcgtgt gggtcagtca ggcccgcctg tcgcgctgtc cgcccaccat gtgccggaga
     2761 ccgatgccgc attcgcgcgc cgggcgcatg gccagaaggg ctcgatcgcc acccggatcg
     2821 aagccgagac cggccgccgg attgcaacga tccgccagac gatagaaccg gtagcgctcg
     2881 gtgcccgcga ggcgagcctg ctgcaggccg gggaaggaga cccggcgctc atgacctggc
     2941 gctggcatct tgatgaccgc gacgtcctgc tgctcgccag caacagcctg ttcccccgcg
     3001 gtcgctatgt ctggagccag accgtgcgcc gccggatcgc gccgcccgaa ctggcgcctg
     3061 tcctgtctgc gaaagagccc cgatgaccgt tccgagcgcc tattttcgac agtccgacgg
     3121 cgcctttcgc gaacgtttcg gactgtcttt cgaggacttt tcggtgggcc agcggttctt
     3181 gcaccgtccc ggcatcacgc tgtcgcagca ggagaacgtc gaggaatccc tcgacacgct
     3241 gaatgcggcc atgctgcact acgatgccca ttatgcggcg cagaccgcct ggaagcgccc
     3301 gctgctggtc tcgacgatca cgttgaaacg cgccatcggc atgggctcga agacgctcgg
     3361 gcgccgccgg gcgatcctgc gcttcgacga gatcgccatg acggcgccgc tgttcggcgg
     3421 ggatacgatc tattccgaat ccgaggtgct ggatatcgag cccggcgacc ggtccgactg
     3481 cggcaccgtc aggctgcgca ccagcgccgt caaggcggat gggatcgtgg ccggccgctt
     3541 cgtctggctg ggtgcgttct ggcggcgcgg ccatgggccg gacgcggaac ctggaactcc
     3601 ctgcgaaacc gcgcgttttt cggcctttcg taagctagag aacggcgcgc tcatggaaga
     3661 gcaaggtctt ttctacgagg atttcgagga aggcgactgc tttatccact atccgcggcg
     3721 gagtttttcc gaagtcgaac tgatcgagca gacgcgccgc gcgctcgacc tctcgccatt
     3781 ctggcaggac cctgcccagc gcagcgagac tggcgcgcta cgggtgccag aacccgtgct
     3841 gatcggcgcc gcgaccgcac tcaccacgcg cacctttggc cgtgtcgtgg ccaatctggg
     3901 ctggaccgac acggaactgc ccgctcccgt gttcgctggc gacacgatcg aagcccgctc
     3961 ccgcattgtc gcccggcgcg attccaggtc ccggcccggc gaaggcgtgc tgaccgtcga
     4021 taccagcgtg cgcaaccaac gggatgtcac cgtcctgtcg tttcggcgca atctcctggt
     4081 ctaccggcgc agcagcgcgc cctatgcaca agcgggatac tgacgtcagt ttcgctcccg
     4141 aaggcggaag tcgggaaagc gtatccccat gcgcgtgctg ccttcggcgg cgagatgtgc
     4201 cttgtcgcga tagagaaacg cgccgtccag cgtggcgctg cagcggtcct gcgggcagag
     4261 cagacgtcgc agcgcaatga cgtggacgcc ctcgccttcg agcgaacgca ggaaggcatc
     4321 ga
//
LOCUS       DAOMXN010000214         4306 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_11007_length_4306_cov_9.467419, whole genome shotgun sequence.
ACCESSION   DAOMXN010000214 DAOMXN010000000
VERSION     DAOMXN010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4306
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11007_length_4306_cov_9.467419"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /gene="rsmH"
                     /locus_tag="PK812_12170"
     CDS             complement(<1..884)
                     /gene="rsmH"
                     /locus_tag="PK812_12170"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRJ70350.1"
                     /translation="MMTGSGAGTPGAAGGPARHIPVLRREALDALGLKPGGRYVDGTF
                     GAGGYSRAILDAFPDTRVLAIDRDPNAVRDGQELVASYGGRLTLVQGRFGDLDAIVEE
                     SGFGAPDGVVLDIGVSSMQLDEAIRGFSFRNDGPLDMRMEQEGTSAADIVNAADEVEL
                     ANILYHFGEERLSRPIARALVGVRKTHPFETTRQLAEAVARVVRSKPGEIHPATRTFQ
                     ALRIAVNDELGELVRALHAAERALPPGGRLAVVSFHSLEDRIVKVFLAARSGRGGGSR
                     HAPIAVTGAPTFRIDGKWP"
     gene            complement(881..1351)
                     /locus_tag="PK812_12175"
     CDS             complement(881..1351)
                     /locus_tag="PK812_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HRJ70351.1"
                     /translation="MDRFLAQFSAKLDSKGRVSIPASFRAVLAKDGHEGLFVYRALDQ
                     PALDCGGHGLLKQIDGLLARFDPYSEAYDSFATALLAESEILKLDPEGRVAVTERLKA
                     HAGIADAVVFVGKQSKFQIWEPERFRAHSEEAKTRLRAYRQSLEPGSNSSGAQG"
     gene            1657..2625
                     /gene="speB"
                     /locus_tag="PK812_12180"
     CDS             1657..2625
                     /gene="speB"
                     /locus_tag="PK812_12180"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015915137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HRJ70352.1"
                     /translation="MSAKSPFNQPVSGLVTPRFAGIATFMRLPHVPLTRARGIDIGLV
                     GVPFDGGTTNRPGPRHGPRQVREMSQMVRRMHPDSHIVPYELANCADLGDCPVNPADL
                     KDAVKRIEKFYRTLVAKGIRPLSAGGDHLISWPILRALGAEHPVGMVHFDAHTDLFAG
                     YFGGFTVTHGTPFRRAIEDGVLDPKRTVQIGIRGSMYDNEDRDFAASVGVRIIDIEEF
                     HDLGIDRTMEIARKIVGGGPTYVSFDIDALDPAYAPGTGTPEIGGFTNREAQFMLRKL
                     RGVNIVGADMVEVSPPFDPSGMTAYNGATMMWEILCLMSEDVAKRR"
     gene            2726..3535
                     /locus_tag="PK812_12185"
     CDS             2726..3535
                     /locus_tag="PK812_12185"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HRJ70353.1"
                     /translation="MAKALSGRTALVTGSTSGIGLAIAKALAGEGARIAINGFGTPEQ
                     IADAEAALKAAGAPEVKYFNADLTDQPAIDRMMDAVLAWAGKLDILVNNAGMQVTASI
                     ATMPVETWQKIIALNLSACFYTLRRALPGMMERGFGRVINIASVHGLVASVEKAPYVS
                     SKFGLVGLTKVAALEAAPAGTRDSGGVTVNAICPGWVETALIEPQIQARAAKFSGDRE
                     AGVRDLVKEKEPSQRMATPDDIGALALFLCSNAAHNITGAAIPVDGAWTAQ"
     gene            3824..4096
                     /locus_tag="PK812_12190"
     CDS             3824..4096
                     /locus_tag="PK812_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein usg"
                     /protein_id="HRJ70354.1"
                     /translation="MSVTPAFRQQMKGYGLTTAQILYRMPDYRNLLQEYIWQDYDLAP
                     HFPELKKFLEFWQDNLEGPLVSVTICQAEKIGPRKFGKVDGLFLLH"
BASE COUNT          747 a         1531 c         1320 g          708 t
ORIGIN      
        1 ggccacttcc cgtcgatccg gaatgtcggc gcgccggtca ccgcgatcgg cgcgtggcgc
       61 gaaccgccgc cgcgccccga gcgggccgcg aggaacacct tgacgatacg gtcttccagc
      121 gaatggaagc tgaccacggc caggcgccct ccgggcggca gggcgcgttc tgccgcgtgc
      181 agggcacgca ccagttcacc cagttcgtcg ttcaccgcga tgcgcagggc ctggaacgtc
      241 cgcgtggccg ggtggatttc gcccggcttc gagcgcacga cgcgggccac ggcctcggcc
      301 agctggcgcg tggtttcgaa cggatgcgtc ttgcgaaccc cgacgagcgc gcgggcgatc
      361 gggcgcgaca ggcgctcctc gccgaaatgg tagaggatgt tggcaagctc gacctcatcc
      421 gccgcgttga cgatgtccgc ggcgctggtc ccctcctgct ccatccgcat gtcgagcggc
      481 ccgtcgttgc ggaaggaaaa gccgcgtatg gcctcatcca gctgcattga ggaaacgccg
      541 atgtcgagca cgacaccgtc cggcgcgccg aacccgctct cctcgacgat cgcatcgaga
      601 tcgccgaacc ggccttgcac cagcgtcagt cggccgccgt acgacgcgac cagttcctgg
      661 ccatcgcgca ccgcattggg gtcgcggtcg atggcgagca cgcgcgtgtc gggaaaggcg
      721 tcaagaatgg cgcggctgta gccgccggcc ccgaaggtcc cgtcgacgta gcgtccgccg
      781 ggcttcagcc ccaacgcatc gagagcctcc cggcgcagca ccggaatgtg acgggcgggt
      841 ccgccggcgg cgccgggtgt tccagcgccg ctgcccgtca tcatccctgt gctccactcg
      901 agttcgatcc gggttccagc gattggcgat aggcgcgcag ccgggtcttg gcctcttccg
      961 aatgcgcgcg gaaccgctcc ggctcccaga tctggaactt cgactgcttg cccacgaaca
     1021 ccaccgcatc ggcgatgccg gcgtgtgcct tcagccgttc cgtgaccgcg acccggccct
     1081 ccggatcgag cttcaggatc tcgctctcgg ccagaagcgc agtcgcaaag ctgtcgtagg
     1141 cctccgagta cggatcgaac cgcgcgagca gcccatcgat ctgtttcagg agcccatgac
     1201 cgccgcagtc cagcgccggc tgatcgagcg cccggtagac gaacagcccc tcgtgcccgt
     1261 ccttcgccag aacggcccgg aacgatgccg gaatcgacac cctgcccttg ctgtccagct
     1321 ttgcggagaa ctgcgcgaga aaccggtcca tcaccacgcc acggatacgc aacatccccg
     1381 ccctgaacct ggacagcccc cgccacggac gcagcgcgct gcgctgcgcc agtcggttca
     1441 gaccgccaaa ctccgaatgg gatgatttgg gatagcatgg gataatatgg gcgtcaacgc
     1501 gttgcgccac gccgtcaggc gcagctcggg atggaagcgc catcacagac gattaacctt
     1561 aaggaattgt tgaaagtggc cggcgcaggc acgaaagcgc cattgtggaa tcgacggctc
     1621 cccttacgat ggtccgctca gacacctgga gccagcatgt ccgccaaatc cccgttcaac
     1681 cagcccgtct cgggacttgt gacgccgcgt tttgccggca tcgccacctt catgcgcctg
     1741 ccccacgtgc cgctgacgcg ggcaagaggc atcgacatcg ggctcgtcgg cgtgcccttc
     1801 gatggcggca ccaccaaccg ccccggccct cgacacggcc cccgccaggt gcgcgagatg
     1861 tcgcagatgg tgcgccgcat gcaccccgac agccacatcg tgccctacga actcgccaac
     1921 tgcgccgatc ttggcgactg cccggtgaat ccggcggacc tgaaggacgc ggtgaagcgc
     1981 atcgagaagt tctaccgcac gctggtcgcc aaaggcatcc ggccgctctc ggccggcggc
     2041 gatcatctca tttcctggcc gatcctgcgc gcactgggcg ccgaacaccc cgtcggcatg
     2101 gtgcatttcg acgcccacac cgacctgttc gcgggctatt tcggtggctt caccgtgacg
     2161 cacggcacac cgttccgccg cgccatcgag gatggcgtgc tcgatccgaa acgaaccgtg
     2221 cagatcggca tccgcggctc gatgtacgac aacgaggacc gggatttcgc cgcttcggtc
     2281 ggcgtgcgca tcatcgacat cgaggagttc catgacctcg gcatcgaccg gacgatggag
     2341 atcgcccgca agatcgttgg tggcggcccg acctatgtct cgttcgacat cgacgcgctc
     2401 gatccggcct acgcgcccgg caccggcacg cccgagatcg gcggttttac caaccgcgag
     2461 gcgcagttca tgctgcgcaa gctgcgcggc gtgaacatcg tgggagcgga catggtggaa
     2521 gtgtccccgc ccttcgatcc ctccggcatg actgcttaca acggcgccac catgatgtgg
     2581 gagattctct gcctcatgtc cgaggatgtg gccaagcgtc gctgatccga tgcaccggga
     2641 ccaagtgtcg gcttgacggc tcccgggcgc tcggcctacc gcagcgcagc atttctggca
     2701 tcatctccac tgcaaggaac agatcatggc gaaagctctt tccggacgca ccgcgctcgt
     2761 caccggctcc accagcggca tcggccttgc catcgcgaag gcgctcgccg gcgaaggtgc
     2821 ccgcatcgcc atcaacgggt tcggcacacc ggagcagatc gccgacgccg aagccgcgct
     2881 gaaagccgcc ggcgcgccgg aggtgaagta cttcaacgcc gacctcaccg accagcccgc
     2941 aatcgaccgc atgatggacg ccgtgctcgc ctgggccggc aaactcgaca tcctcgtcaa
     3001 caacgcgggc atgcaggtca ccgcctcgat cgcgaccatg ccggttgaga cctggcagaa
     3061 gatcatcgcg ctcaacctct cggcctgttt ctacaccctg cgccgcgccc tgcccggcat
     3121 gatggagcgc ggcttcgggc gtgtcatcaa catcgcctcg gtgcacggcc tcgtcgcctc
     3181 ggtagagaag gcgccatatg tgtccagcaa attcggcctc gtcggcctga ccaaggtcgc
     3241 ggcgctggag gcggccccgg ccggcacgcg ggattccggc ggcgtcacgg tcaacgccat
     3301 ctgcccgggc tgggtggaaa cggcactgat cgagccgcag atccaggccc gtgccgcgaa
     3361 gttttccggc gaccgcgagg cgggcgtgcg cgaccttgtc aaagagaagg agccttccca
     3421 gcgcatggcg acgccggacg acatcggcgc tctcgccctg ttcctctgct cgaacgccgc
     3481 ccacaacatc accggcgcgg cgatcccggt cgatggcgcc tggacggcgc agtgagggcg
     3541 ggaagggcga agtaatcgcc aatcgtcggt gtcattccgg attggcgaag cccggtccgg
     3601 actccatctt tttcaaagct tcgatcgaaa acgacgggtt ccgggctccg cttcgcgacc
     3661 ccggaatgac acctttattg cctattgccg gctgccctct tccccattcg cgattggcca
     3721 ctcaccattc gccccatcaa acccttcctt aacaaaccgc gcgcagcctc atcccatgat
     3781 tgcttgagga acagcgccag cgccggcgca aggaggggtt tcgatgtccg tgacgcccgc
     3841 attccgccag cagatgaaag gctacggcct caccaccgcg cagatcctct atcgcatgcc
     3901 ggattaccgg aacctgctgc aggaatacat ctggcaggac tacgatctcg ccccgcattt
     3961 tccggagctg aagaagttcc tcgaattctg gcaggacaat ctcgaaggcc cgctcgtctc
     4021 cgtcacgatc tgccaggccg agaagatcgg cccgcgcaaa ttcggcaagg tggacggatt
     4081 gttcctgctg cattgagcgg gcgtttcccc ggctggacgc ggcacgcgac cccgcccgcc
     4141 atcgattctc cacaaagccg cgcgcatcct gacgcaccgg gtgtggggcg cccggaacct
     4201 gctcgcaacc ttttggagaa ttccatgctt cgcagcgctt cacgccgcgc aaccgccgcc
     4261 gcccttctgc tggccgccgt tggcgcggcc cccgccctcg cccaga
//
LOCUS       DAOMXN010000215         4276 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_11117_length_4276_cov_11.533523, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000215 DAOMXN010000000
VERSION     DAOMXN010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4276
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11117_length_4276_cov_11.533523"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="PK812_12195"
     CDS             complement(<1..216)
                     /locus_tag="PK812_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70355.1"
                     /translation="MTFTTVPYGPCSADCPRFIQASGTITSATPEAFLAFARRETDRP
                     GLMNVIFIHSTGGSVAASLRLGRMFRSL"
     gene            complement(405..481)
                     /locus_tag="PK812_12200"
     tRNA            complement(405..481)
                     /locus_tag="PK812_12200"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Val,seq:tac)
     gene            complement(529..1146)
                     /locus_tag="PK812_12205"
     CDS             complement(529..1146)
                     /locus_tag="PK812_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70356.1"
                     /translation="MIVPAAALSYSTVEIDDGKCRPNCASAIVATGSINGREVEDLTW
                     FMRHSGKNKRLAKLFIIHSGGGNAYGGLSLGMYLRQAGVTVMVAQTNGEPITRTGGLG
                     RGLCGSACVFVLAGGAKRVVPQGSVVAVHGARPVQTEVHDRMAGTVTSIKVDRDQIAT
                     AFASYYQKMGINPGLAKLAESIPHSEFRVLSPQELSKFRLATTRM"
     gene            complement(1315..3738)
                     /gene="lon"
                     /locus_tag="PK812_12210"
     CDS             complement(1315..3738)
                     /gene="lon"
                     /locus_tag="PK812_12210"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003600393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HRJ70357.1"
                     /translation="MTATKPRPPLVPGSVDTVPVLPLRDIVVFPHMIVPLFVGREKSI
                     RALEEVMKSDRYILLATQKNKDEDDPKADGIYEVGTLATVLQLLKLPDSTVKVLVEGA
                     GRARVRRYVKDDGHLEAEVDVLADDAGDKVEVEALARSVVNEFEGYVKLNKKLSPDVV
                     SAVTQIDDYAKLADTIASHLSVKIADKQAILEMPRVSDRLEKVLGLMESEVSVLQVEK
                     RIRTRVKRQMEKTQREYYLNEQIKAIQKELGDEDGKDEMAELEDRIKRTKLSKEAHDK
                     AMHELKKLRQMSPMSAEATVVRNYLDWMLSLPWGKRSKVKKDLNLAQEVLDADHFGLD
                     KVKERIVEYLAVQQRANKLTGPILCLVGPPGVGKTSLGKSIAKATGREFVRMSLGGVR
                     DEAEIRGHRRTYIGSMPGKIIQSLRKAKTQNPLFLLDEIDKMGMDFRGDPSSALLEVL
                     DPEQNATFADHYLEVDYDLSNIMFVTTANSLNIPAPLMDRMEIIRIAGYTEDEKAEIA
                     RKHLIPNAISKHGLKPEEWSIDAEALLTLVRRYTRESGVRNLEREISNLVRKAVKEIT
                     LKKLVKVDVTAANLADFLGVPKYRYGEAETEDQVGVVTGLAWTEVGGELLTIEGVMMP
                     GKGKMTVTGNLRDVMKESISAAASYVRSRAIDFGVEPPLFDKRDIHVHVPEGATPKDG
                     PSAGIAMATTIVSILTGIPIRRTVAMTGEVTLRGRVLPIGGLKEKLLAALRGGLKTVL
                     IPEDNVKDLAEIPANVKNGLEIIPVSRMDEVLKHALVRQPTPIIWDEELADKAAAKDK
                     SEDSATVAH"
     gene            4011..>4276
                     /locus_tag="PK812_12215"
     CDS             4011..>4276
                     /locus_tag="PK812_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HRJ70358.1"
                     /translation="MKDGAASTNSMSLRILRSPLTRLVLLGGPVFFMMGLSSGFMEKF
                     RGDPVKSIAAVLGMAMAALAFYVAYARLVERRPANELSLHGAGR"
BASE COUNT          727 a         1381 c         1336 g          832 t
ORIGIN      
        1 aaggctgcga aacatccgac cgagcctgag agacgcagcg acgctgccgc ccgtcgaatg
       61 gatgaagatc acgttcatga ggcccggccg gtcggtttcc cgacgggcga aggcgagaaa
      121 cgcctcgggg gtcgccgagg tgatggttcc gctggcctgg atgaaacggg ggcaatcggc
      181 cgaacaggga ccgtagggca ccgttgtaaa ggtcatttcc gctgagcggc ttggcgttgc
      241 gccaagcgca agccaaaccg tgcccaggaa cgcggacaaa cgcaaccgac gcatggaacg
      301 caactctaca gaacgcaacg caactgacgg ctgtgactat gtcggctcac ggttaaaaaa
      361 ccgcagcccc cacaggaaaa cattcgttct gttttggatc gaactggtgg gcggtgagag
      421 attcgaactc ccgacctact gggtgtaaac cagccgctct aaccagctga gctaaccgcc
      481 ctcggcgcgt ctttcactgc atttcgcatc gaaaggcaag cgggccgatc acatccgcgt
      541 ggtcgccagc cggaacttcg acaattcctg cgggctcagc acgcggaact ccgaatgcgg
      601 gatgctttcg gccagtttcg ccagtcccgg attgatgccc atcttctggt aatagctggc
      661 gaaggcagtc gcgatctggt cacgatccac cttgatgctg gtcaccgtgc cggccatgcg
      721 atcgtgcacc tcggtctgca ccggacgggc gccgtgcacc gcgacgacac tgccttgcgg
      781 caccacgcgc ttggcgccgc ccgccagcac gaacacgcaa gccgagccgc agaggccccg
      841 tccgaggcct cccgtcctgg tgatcggctc gccattcgtc tgggcgacca tgacggtcac
      901 gccggcctgg cgcaagtaca tgccgagcga cagaccgcca taggcgttgc cgcctcccga
      961 atgaatgatg aagagcttgg cgaggcgctt gttctttcca ctgtggcgca tgaaccaggt
     1021 gaggtcctcg acctcgcggc cgttgatgct cccggtggcg acaatggcgg atgcgcagtt
     1081 cggacggcac ttgccgtcat cgatctcgac cgtgctgtaa ctgagcgccg cggccggtac
     1141 gatcaagcca agccacgcag cggcggcaat tccaagacaa cgcaactgtc ccatggacgc
     1201 acccggtcgg ttaaccaatg ttaacagtca agacgagcgc tgccgttgcg tcaacaaaaa
     1261 ccccgcgccg tgcgcggggt tcctgcattc gatgacggat atgcttaaca gccgtcagtg
     1321 cgccactgtt gcactgtcct ctgacttgtc cttggccgcc gccttgtcgg ccagttcctc
     1381 gtcccagatg atcggcgtcg gctgacgcac aagcgcgtgc ttgagcacct cgtccatgcg
     1441 cgagaccggg atgatctcca gcccgttctt cacgttggcc gggatctcgg cgagatcctt
     1501 gacgttgtcc tcggggatca gcacggtctt gagcccgccc cgcagcgccg ccagcagctt
     1561 ctccttgagc ccgccgatgg gcagcaccct gccccgcagc gtgacctcgc cggtcatggc
     1621 gaccgtgcgc cggatcggaa tgccggtgag gatcgacacg atggtcgtcg ccatggcgat
     1681 gcccgccgaa gggccatcct tgggcgtcgc gccttccggc acgtgcacgt ggatgtcgcg
     1741 cttgtcgaac agcggcggct cgacgccgaa atcgatggcg cgcgagcgga cataggaggc
     1801 cgccgccgag atcgattcct tcatcacgtc gcgcaggttg cccgtgacgg tcatcttgcc
     1861 cttgccgggc atcatgacgc cttcgatggt gagcagttcg ccgccgacct cggtccaggc
     1921 cagcccggtg acgacgccga cctgatcctc ggtctccgcc tcgccgtagc ggtacttcgg
     1981 cacgcccagg aaatcggcga ggttggcagc agtaacgtcc accttgacca gcttcttgag
     2041 cgtgatttcc ttcaccgcct tgcgcacgag gttggaaatc tcgcgttcga ggttgcgcac
     2101 gcccgattcc cgcgtgtagc ggcgcaccag ggtgagcagc gcctccgcgt cgatggacca
     2161 ttcctcaggc ttcaggccgt gcttggaaat cgcgttcggg atcaggtgct tgcgcgcgat
     2221 ctcggccttc tcgtcctcgg tgtagccggc gatgcggatg atttccatgc ggtccatcag
     2281 cggcgcgggg atgttcagcg agttcgccgt ggtcacgaac atgatgttcg acaggtcgta
     2341 gtccacttcc agatagtggt ccgcgaaggt cgcgttctgt tcgggatcga gcacctccag
     2401 caaggcggac gacggatcac cgcggaagtc catgcccatc ttgtcgatct cgtcgagcag
     2461 gaagagcgga ttctgcgtct tggccttgcg cagggactgg atgatcttgc caggcatcga
     2521 gccgatgtag gtgcgccggt ggccgcggat ttccgcctcg tcgcgcacgc cgccgagcga
     2581 catgcgcacg aactcgcggc cggtcgcctt ggcgatcgac ttgccgagcg aggtcttgcc
     2641 gacgccgggc gggccgacaa ggcacaggat cgggccggtg agcttgttgg cgcgctgctg
     2701 cacggcgaga tactcgacga tgcgctcctt gaccttgtcg agaccgaagt gatccgcatc
     2761 caggacttcc tgggcgaggt tcaggtcctt cttcaccttg gagcgcttgc cccagggcag
     2821 cgagagcatc cagtcaaggt agttgcgcac cacggtggct tccgccgaca tcggcgacat
     2881 ctgcctgagc ttcttgagct cgtgcatcgc cttgtcatgc gcttccttgg agagcttggt
     2941 gcgcttgatg cggtcttcga gttcggccat ctcgtccttg ccgtcctcgt cgcccagctc
     3001 cttctggatc gccttgatct gctcgttgag gtagtattcg cgctgtgtct tctccatctg
     3061 gcgcttgacg cgcgtgcgga tgcgcttctc gacctggaga acggaaacct cgctctccat
     3121 caggccgagc actttctcca accggtccga caccctgggc atctccagga tcgcctgctt
     3181 gtcggcgatc ttcaccgaca ggtgggaggc gatggtgtcg gccagcttgg cgtagtcgtc
     3241 gatctgcgtg accgcgctga cgacatcggg cgaaagcttc ttgttgagct tcacatagcc
     3301 ttcgaactcg ttgacgaccg agcgcgccag cgcctcgacc tcgaccttgt cgccggcgtc
     3361 atcggccagc acgtccacct cggcctccag atggccatcg tcctttacat accgacgcac
     3421 gcgcgcgcgc ccggcgccct cgaccagcac cttcacggtg ctgtccggca gcttcaggag
     3481 ctggaggacc gtcgccagcg tgccgacctc gtagatgccg tccgccttcg ggtcgtcctc
     3541 gtccttgttc ttctgcgtgg cgagaaggat gtagcgatcc gacttcatca cctcttcgag
     3601 ggcgcggatc gacttctcgc ggccgacgaa cagcggcacg atcatgtgcg ggaagacgac
     3661 gatgtccctg agcggcagca ccggcaccgt gtcgacggaa ccgggaacga gcggaggacg
     3721 cggcttggta gcggtcattt cttgatccct ctggtgccgc gggcggacaa gcccgccccg
     3781 gctctcgacg acgccgacac cgatcccgct gcaagcaaca cgcgaaaccg gcagaacccg
     3841 cgactcagtg gcgttcccgc cgccgtctcc ggctaaggtg ggggggaacg gttaaggatt
     3901 caaggtgggt ggtctgttca tgactgcaat cgggattccg ctaccctgcc aagctggtca
     3961 ggtcctggta tgccgggtat cctgtgatgt tggcccagaa ggaggcagcg atgaaagacg
     4021 gcgcggcgtc gaccaattca atgtcattgc gcatcctgcg ttctccgctc acacgcctcg
     4081 tcctcctggg cgggcccgtg tttttcatga tgggcttgag cagtggtttc atggagaaat
     4141 tcaggggcga tcccgtgaag tccatcgccg ccgtgttggg catggccatg gccgccctcg
     4201 ccttctacgt ggcatatgcg cggctggtcg agcggcgtcc agccaacgaa ctctcgctcc
     4261 atggcgccgg tcggga
//
LOCUS       DAOMXN010000216         4276 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_11118_length_4276_cov_10.217247, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000216 DAOMXN010000000
VERSION     DAOMXN010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4276
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11118_length_4276_cov_10.217247"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="PK812_12220"
     CDS             complement(<1..647)
                     /locus_tag="PK812_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70359.1"
                     /translation="MSLPRIRLDFGLSKLIPGAQKAAPEPIPSNAIAFPLAGAAPFDG
                     YPNHISETAPANLPGWTISGLHRRIPADLAALLSPDPASALRTVTALVDRLDYYRARM
                     AEYQERLSTGAKGSASIENYIRLVYIQLFNELATAFRLDLTQDGIADVRAQAFVRVIR
                     QVKLLTTADLIKVADLIQDEVLNAIIARSDWIRRGEDGPVAASQPTAPMAPVPARP"
     gene            complement(782..1246)
                     /locus_tag="PK812_12225"
     CDS             complement(782..1246)
                     /locus_tag="PK812_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008875010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HRJ70360.1"
                     /translation="MSATPTVRVQAGDFDPAEETRLLTADRADIGAVVTFAGLCRDEG
                     GTLAALELEHYPGMAEDEMMRVAREAAMRWPLQGLTVIHRYGRIAPGENIVLVVAASA
                     HRKAAFEAADFLMDYLKTRAPFWKKEHRRDGAAGDWVAAKVADDEAAERWGA"
     gene            complement(1248..1499)
                     /gene="moaD"
                     /locus_tag="PK812_12230"
     CDS             complement(1248..1499)
                     /gene="moaD"
                     /locus_tag="PK812_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009339908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HRJ70361.1"
                     /translation="MKLVYFAWVRERIGKPDERVDLPAGLTTVADLLAWMQGRGEEYA
                     HAFEKASLIRVAIDKRHVKHDAPLAGAREIAFFPPMTGG"
     gene            complement(1496..2095)
                     /gene="pgsA"
                     /locus_tag="PK812_12235"
     CDS             complement(1496..2095)
                     /gene="pgsA"
                     /locus_tag="PK812_12235"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018044663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HRJ70362.1"
                     /translation="MPSARKSPFNLPNILTYGRILAIPALVALLFWPEEFWLRWTALA
                     IYVTAGVTDYLDGYLARAWSQQSALGRMLDPIADKLLVAASLLMLVSDGTIRSWALWA
                     AIIILCREVLVSGLREFLAEVRVSIPVSQLAKWKTTGQLVAIGFLIAGPAGDALLPKG
                     WTVRIGLTLLWIAAVITLYTGYDYLKAGIRHILEQDAEE"
     gene            complement(2196..4184)
                     /gene="uvrC"
                     /locus_tag="PK812_12240"
     CDS             complement(2196..4184)
                     /gene="uvrC"
                     /locus_tag="PK812_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HRJ70363.1"
                     /translation="MARQPHDMPPEDPEADEPEAPLTAEDTLDLSDDATPAALSMGVE
                     VIRAIWKTLPNGPGVYRMAGEGGEVLYVGKAKSLKKRVASYTRGVGHTNRIARMIAAT
                     RAMEFVTTETETEALLLETNYIKQLKPRFNVLMRDDKSFPYILVTGDHAAPQIAKHRG
                     ARGRKGDYFGPFASAQAVGRTINALQRAFLLRSCSDSYYENRSRPCLLYQIKRCAGPC
                     TGETSIADYATLVKEAKDFLTGKSVAVKQRIAAEMQAASDDLEFERAARYRDRLAALS
                     AIQSTQDINPRETEEADVFAVHEEAGQFIIEAFFFRAYQNWGNRAFYPRADRSLSPAE
                     VLDAFLGQFYDERPAARLILISHDIENRALLAEALATRAGHRVEIAVPKRGEKKDLVD
                     HAATNAREALSRRLADTASQQKLLAALGQAFGLEKPPRRIDVFDNSHIMGANAVGAMI
                     VAGEQGFMKTHYRTFNIRAQDLTPGDDYGMMREVLSRRFGRLQKEAARDDGEPPRDTE
                     AFPAWPDLVLIDGGKGQLDAARSVLADLGVAGVPLVGIAKGPDRDAGRETFFIDGRAP
                     FKLPPRDSALYFVQRLRDEAHRFAIGTHRAKRKRELVKNPLDEVPGIGPRRKRALLLH
                     FGTAKAASRASLDDLAKCPGVNAATAKAVYDFFHERQG"
BASE COUNT          676 a         1348 c         1527 g          725 t
ORIGIN      
        1 ggccgcgcgg gaaccggcgc catcggcgcg gttggctgcg aggcggcgac tgggccgtcc
       61 tcgccgcgac ggatccagtc ggagcgggcg atgatggcgt tgagcacctc gtcctggatc
      121 agatcggcca ccttgatcag atccgccgtg gtcaggagct tcacctggcg gatgacgcgc
      181 acgaaggcct gtgcgcggac gtcggcgatc ccatcctgcg tcaggtcaag gcggaacgcc
      241 gtcgccagtt cgttgaacaa ctggatgtag acgaggcgga tgtagttctc gatggaggcg
      301 ctgcccttgg cgccggtcga caggcgttcc tgatattcgg ccatgcgcgc gcggtagtag
      361 tcgagccggt ccacgagcgc ggtcacggtg cgcaacgcgg aagcggggtc cggcgagagc
      421 aacgcggcga ggtccgccgg gatgcgtcgg tgcagccccg agatggtcca gcccggcagg
      481 ttggccggcg cggtttcgga aatgtggttg ggatagccgt cgaagggcgc ggcgccggcc
      541 agcgggaagg cgatggcgtt ggaagggatt ggctccggcg cggccttctg cgcgccagga
      601 atcagtttcg aaaggccgaa atccagcctg atgcgcggca aactcatgaa tgcaacccgc
      661 ccggtccgat aagcttcagc gcgagaccct agagcaaagg cgcaaaggaa cgctgaagcg
      721 agagggttga cgctgggtaa gcgtcatttg cgggattgaa cggaatggcc gtggcggagc
      781 gctatgcgcc ccagcgttcc gcagcctcgt catcggcgac tttcgccgcc acccagtcgc
      841 cggcggcgcc gtcgcggcga tgctccttct tccagaacgg agcgcgggtc ttgaggtagt
      901 ccatcaggaa gtcggcggcc tcgaaggcgg ccttccgatg ggcggatgcc gcgaccacca
      961 gcacgatgtt ttcgcccggc gcgatccggc cataacgatg aatgacagtc agtccctgca
     1021 gcggccagcg catcgccgcc tcgcgcgcga cgcgcatcat ctcatcctcg gccatgccgg
     1081 gataatgttc gagttctagc gcggcgaggg tgccgccttc gtcgcggcac aggccggcga
     1141 aggtcacgac cgcgccgatg tccgcgcggt cggccgtcag caggcgggtt tcttctgccg
     1201 gatcgaagtc gccggcctgc actctgaccg tcggagtggc cgacatgtca gcctcccgtc
     1261 atcggcggga agaaggcgat ctcccgcgct cccgccagcg gggcgtcgtg cttcacgtgg
     1321 cgcttgtcga tggcgacgcg gatcagcgag gccttttcga aggcatgggc gtattcctct
     1381 ccgcgcccct gcatccaggc gagaagatcg gccacggtcg tcaggccggc cggcaggtcg
     1441 acgcgttcgt ccggcttgcc gatgcgttcg cgcacccagg cgaaatagac gagcttcatt
     1501 cctcggcatc ctgctccagg atatggcgga tgcccgcctt caggtagtca tagccagtat
     1561 acagggtgat gacggcggcg atccagagca gcgtcaggcc aatccggacg gtccagccct
     1621 tgggcagcag cgcgtcgccg gccggtccgg caataagaaa gccgatggcg acgagctgtc
     1681 ccgtggtctt ccatttcgcc agctgcgaga cgggtatcga cacccgcacc tcggccagaa
     1741 actcgcgcag gcccgagacc agaacctcgc gacagaggat gatgatcgcc gcccagagtg
     1801 cccaggagcg tatggtgccg tccgagacca gcatcagcag gctggcggcg accaggagct
     1861 tgtcggcgat cggatcgagc atgcgcccca gcgccgactg ttgcgaccag gcccgcgcta
     1921 gatagccgtc gaggtagtcc gtgacgccgg cggtgacata gatcgccagg gccgtccagc
     1981 gcagccagaa ttcctccggc cagaacagca gcgcgaccag cgccggtatc gcgaggatgc
     2041 ggccgtaggt caggatgttg ggcaggttga aagggctctt tcgggcggag ggcatcggcg
     2101 tccggtgtca ggttcgctcg caccgaacac aggcgttgcg cctcgtcaag gcaaggctgt
     2161 gcgaaccggc gcgaaatccg ccggcttgtc gcgcctcacc cctggcgttc gtggaaaaag
     2221 tcgtagaccg ccttcgccgt ggcggcgttg acgccggggc atttggccag atcgtccagc
     2281 gaggcgcggc ttgccgcctt ggccgtgccg aaatgcaaga gcagggcgcg cttgcggcgc
     2341 gggccgatgc cgggcacctc gtccagcggg ttctttacca gctcgcgctt gcgcttggcg
     2401 cgatgcgtgc cgatggcgaa gcgatgcgct tcgtcgcgca gacgctgcac gaagtagagc
     2461 gcggagtcgc gcggcggcag cttgaacggc gcccgaccgt cgatgaagaa ggtttcgcgg
     2521 ccggcgtcgc ggtccggacc cttggcgatg ccgaccagcg gcacgccagc cacgccgaga
     2581 tcggccagca cgctccgcgc cgcgtcgagc tggcccttgc cgccgtcgat cagcacgagg
     2641 tcaggccagg ccggaaaggc ttcggtgtcc cgaggcggtt cgccatcgtc gcgggcggct
     2701 tccttttgca atcggccgaa gcgccgcgac agcacctcgc gcatcatgcc gtagtcgtcg
     2761 ccgggcgtca gatcctgcgc ccggatattg aaggtccggt agtgcgtttt catgaagccc
     2821 tgctcgccgg cgacgatcat cgcgccgacg gcattggcgc ccatgatgtg cgaattgtcg
     2881 aagacgtcga tgcggcgcgg cggcttctcc agtccgaacg cctgcccgag cgccgccagc
     2941 agcttctgct ggctggcggt gtcggcgagg cggcgcgaca gcgcctcgcg ggcgttggtg
     3001 gcggcgtgat ctaccagatc cttcttctcg ccccgcttcg gcacggcgat ctcgacgcgg
     3061 tggccggcgc gcgtcgccag ggcttcggcg agcagggcgc ggttctcgat gtcgtgactg
     3121 atgaggatga gccgcgccgc gggccgttca tcgtagaact ggccgagaaa ggcatcgagc
     3181 acttcggcgg gcgacaggga ccggtcggcg cgcgggtaga aggcgcggtt gccccagttc
     3241 tggtaggcgc ggaagaagaa ggcctcgatg atgaactgcc cggcctcctc gtgcacggcg
     3301 aagacatcgg cctcctcggt ctcgcgcggg ttgatgtcct gcgtcgactg gatcgccgag
     3361 agcgcggcca gccggtcgcg gtagcgcgcc gcgcgttcga attcgagatc gtccgaggcg
     3421 gcctgcattt ccgcggcgat ccgctgcttc acggccaccg acttgccggt gaggaagtcc
     3481 ttcgcctcct tcaccagcgt ggcgtaatcc gcgatcgagg tctcgccggt acaggggccg
     3541 gcgcagcgtt tgatctggta gagcaggcaa gggcgtgagc ggttctcgta gtagctgtcg
     3601 ctgcaggagc gcaacaggaa ggcgcgctgc aacgcgttta tggtgcgccc cactgcctgc
     3661 gcgctggcga acgggccgaa gtagtcaccc ttgcggccgc gtgcgccgcg atgcttggcg
     3721 atctggggcg cggcatgatc gccggtgacg aggatgtagg ggaacgactt gtcgtcccgc
     3781 atcagcacgt tgaagcgggg cttcagctgc ttgatgtagt tggtctccag cagcagcgcc
     3841 tcggtttccg tctcggtggt gacgaattcc atggcacggg tcgcggcgat catgcgcgcg
     3901 atgcggttgg tgtggccgac gccgcgggta tagctggcga cgcgcttctt caggctcttg
     3961 gccttgccga catagagcac ctcgccgcct tcgccggcca tgcggtagac gccgggtccg
     4021 ttgggcaatg tcttccagat ggcgcggatc acctcgacgc ccatgctcag ggcggccggg
     4081 gtcgcatcgt cggaaaggtc aagcgtgtcc tcggccgtaa gcggggcttc cggctcgtcg
     4141 gcctccgggt cctcgggggg catgtcgtgg ggttggcgcg ccatcattgt gagataggcg
     4201 tttcgcgcgc cggggaaaag ccgccctgtg cgcgaacgca catcgaaggg ttgggagtgg
     4261 ggttaggttc caatca
//
LOCUS       DAOMXN010000217         4272 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_11130_length_4272_cov_10.120465, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000217 DAOMXN010000000
VERSION     DAOMXN010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11130_length_4272_cov_10.120465"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1601
                     /gene="xdhB"
                     /locus_tag="PK812_12245"
     CDS             <1..1601
                     /gene="xdhB"
                     /locus_tag="PK812_12245"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007068674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdopterin binding
                     subunit"
                     /protein_id="HRJ70364.1"
                     /translation="GGKESQASQWGALAALAARVTGRPCKLRLDRDDDFVLTGKRHDF
                     GVRWTTGHDGDGRIVGHDIVYDARCGYSAELSQGVIDRTMFHADSAYFLPSTTILTRR
                     LKTNTVSNTAFRGFGGPQGVLAMETVIDRIAQSLGRDPLDVRKANFYGAGRDVTPYGQ
                     TVTDCDTLLPLVETLESTSQYRARRSDIARFNAASPILKKGIALTPVKFGISFTLMHL
                     NQAGALVHVFADGSISINHGGTEMGQGLYVKVAQVVAEEFGVPLAQVKITATTTDKVP
                     NAGPTAASAGSDLNGMAAQAAAVQIRARMAEVAAQMLEAPVEAVVFRDGAVASGDRRM
                     TFAELAKACWLRRVSLSQTGFYATPEIHWDRAKASGRPFLYFAYGACCAEVILDTMTG
                     ENRVTRVDILHDCGRSLNPAIDIGQIEGGFVQGMGWLTTEELVFDAKGRLRTHAPATY
                     KIPVASDVPEDFRVSLWDAENRAPTIYRSKAVGEPPFILGVAVFSALADAVHSLRPGT
                     PVALDAPATPEALMRAVRVSRGAA"
     gene            1598..2527
                     /gene="xdhC"
                     /locus_tag="PK812_12250"
     CDS             1598..2527
                     /gene="xdhC"
                     /locus_tag="PK812_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory protein XdhC"
                     /protein_id="HRJ70365.1"
                     /translation="MKLWSEIAAALERDGFAALVTMKEVRGSSPREAGARMLVRRDGG
                     FRGSIGGGALEHETLTDALRLLEQGRPTALDRQQALGPDLGQCCGGHVRVAIEVFVRD
                     DLVWVRPLAVAEGADGVLETLGRPDGAGRLLRQVAIPSAEGVREIFGIRRTPVFLFGA
                     GHVGRAVALALGPLPFRVDWIDSRADAFPERVPGNATPRHASDPAALVANVLPGTLAL
                     VMTHSHPLDLAIVSAALASGAFPYVGLIGSATKRARFTSQLRQIGLSEAQLGPLACPI
                     GDGDVRDKSPAAIAAVAAVELLRWRERTAPHRL"
     gene            2597..3562
                     /locus_tag="PK812_12255"
     CDS             2597..3562
                     /locus_tag="PK812_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018238534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HRJ70366.1"
                     /translation="MKVRVLLAAAFAVLAATASAQDYPNRPVTLVVPFAAGGPSDIIG
                     RLLAQSMTQTLKQQVIVENTAGAGGTLAINRVAKAAPDGYTILISHVNHAATAILYRK
                     LQYDPVEDFAPIGLATDGAMAIIGKKGIAPNTIQELMAHIKEQKDKVSYAHAGIGSGA
                     HLCGLQLMSALQTQMTVVTYRGTGPALNDLVGGQIDLLCDQVTSALPQIQAGTVKAYA
                     VTTKEPLKSLPLPSMEQVGIRGFEVNVWHGLYAPKGTPAPIVERLASALRAALKDPLV
                     LKRLDDLSTTPVSDERATPDALKKHLVSEIAKWRPIIQGAGQYAD"
     gene            complement(3599..>4272)
                     /locus_tag="PK812_12260"
     CDS             complement(3599..>4272)
                     /locus_tag="PK812_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HRJ70367.1"
                     /translation="MAQLPAEDIARKLSDCRLTQALFNVYPGDFARGERGFAALPERA
                     SDLSASLPLALRYAQATGARRLHLMAGIADRNDPRCVARFGDSLKEAAQVFGPHGIDV
                     LIEPLNSRDNPGYFLTDFALAATTIDALKLPNLKLQFDIYHRQILHGDVTKALAAMMP
                     IIGHVQTASVPLRHEPGTGELDDGFVFATLDRLGYDGYVGCEYRPRAGTLAGLGWITP
                     YRARK"
BASE COUNT          672 a         1380 c         1500 g          720 t
ORIGIN      
        1 tcggcggcaa ggagagccag gccagccaat ggggcgcgct ggcggcgctg gccgcgcgcg
       61 taacgggccg gccgtgcaaa ctcaggctcg accgtgacga tgatttcgtg ctcaccggca
      121 agcgtcacga tttcggcgtg cgctggacca ccggccacga cggcgacggc aggattgtcg
      181 ggcacgacat cgtctatgac gcgcgctgcg gctattcggc cgaactgtcg cagggcgtga
      241 tcgaccggac gatgttccac gccgacagcg cctatttcct gcccagtacc acgatcctga
      301 cccggcggct gaagaccaac acggtgtcca acaccgcgtt tcgcggcttc ggcggtccgc
      361 agggcgtgct ggcgatggaa acggtcattg atcgcatcgc ccagtcgctc gggcgcgatc
      421 cgctcgatgt gcgcaaggcg aatttttatg gcgcgggacg ggatgtgacg ccctacggcc
      481 agaccgtcac ggattgcgac accttgctgc cgctggtgga aaccctggag tcgaccagcc
      541 agtaccgggc ccggcgctcc gacatcgcgc gcttcaacgc cgcctcgccg atcctgaaga
      601 agggcatcgc cctgacgccg gtcaaattcg gcatctcttt caccctgatg cacctcaacc
      661 aggccggcgc gctcgtgcac gtgttcgccg acggctcgat ttccataaac cacggcggca
      721 ccgagatggg gcaggggctc tacgtcaagg tggcgcaggt ggtggccgag gaattcggcg
      781 taccgctggc gcaggtgaag atcaccgcca cgaccacgga caaggtgccg aacgccggac
      841 cgacggcggc ctcggcggga tcggacctca atggcatggc ggcgcaggcc gccgccgtgc
      901 agatccgtgc ccgcatggcc gaggtggcgg cgcagatgct cgaggcgccg gtcgaggcgg
      961 tcgtgttccg cgatggtgcc gttgcgtccg gcgatcggcg catgactttc gcggaactgg
     1021 ccaaggcctg ctggctccgg cgcgtatctc tgtcgcagac cggtttttac gcgacgccgg
     1081 aaatccactg ggatcgggcg aaggcgagcg gccggccctt cctctatttc gcctatggcg
     1141 cctgctgtgc cgaggtcatc ctcgatacga tgaccggcga gaacagggtg acgcgcgtcg
     1201 acatcctgca cgattgcggg cgctcgctga atccggccat cgacatcggc cagatcgagg
     1261 gcggattcgt gcagggcatg ggctggttga ccaccgagga actggtgttc gacgccaagg
     1321 gccggctgcg cacccatgcg ccggccacct acaagattcc ggtcgcctcc gatgtgccgg
     1381 aggactttcg cgtctcgctg tgggacgcgg agaaccgcgc gccgacgatc tatcggtcaa
     1441 aggcggtggg tgagccgccg ttcatactcg gcgtcgccgt gttctcggcg ctggcggacg
     1501 cggtccattc gctgcggccg gggacccctg tggcgctcga tgcgccggcc acgcccgagg
     1561 cgctgatgcg cgccgtgcgg gtgtcgcggg gcgcggcttg aagctctggt ccgagattgc
     1621 cgccgcgctg gagcgcgatg gcttcgccgc tctcgtcacc atgaaggaag tgcgcgggtc
     1681 cagtccgcgc gaagccggcg cacgcatgct ggtccgccgc gatggaggat ttcgcggctc
     1741 gatcggtggc ggggctctgg aacacgagac gctgacggat gcgttgcgcc tgctggagca
     1801 gggaaggcct accgcgctcg accggcagca agcactcgga cccgatctcg gccagtgctg
     1861 cggcgggcat gtgcgtgttg cgatcgaggt cttcgtgcgc gatgacctcg tctgggtccg
     1921 gccactggcg gtggccgagg gcgcggatgg cgtgctcgaa acgctcggca ggccggatgg
     1981 cgcggggcgg ctgttgcggc aggtcgcgat cccttccgcc gaaggcgtgc gggaaatctt
     2041 cggcattcgg cgaacgcccg tgttcctgtt cggcgcgggc catgtggggc gggcggtggc
     2101 gctggcgctc ggtccgctgc cgttccgcgt cgactggatc gattcgcgcg ccgatgcttt
     2161 tcccgaacgg gtaccgggca acgccacgcc ccgccatgcc tccgatccgg ccgcgctcgt
     2221 cgccaatgtc cttcccggaa cgctggcgct ggtgatgacc cacagccatc cgctggatct
     2281 tgccatcgtt tcggccgcgc ttgcgagcgg cgcctttccc tatgtcggtc tgatcggcag
     2341 cgcgacgaag cgggcgcgct ttaccagcca gttgcgccag atcggcctgt ccgaggcgca
     2401 actgggcccg cttgcttgtc ccatcggtga cggtgatgtg cgcgacaaga gccccgccgc
     2461 catcgccgcg gtggcggcgg tcgaattgct gcgctggcgg gagcgaacgg cgccgcaccg
     2521 gctttgacct ttgcgcgtcg acgcggttag atgatctcag agcgtcggaa caggcgcgtc
     2581 ttggagggag gatttcatga aggttcgcgt gctacttgca gcggcgttcg cggtgcttgc
     2641 ggccacggct tcagcccagg attatccgaa ccggccggta acgctggtcg tgccgttcgc
     2701 ggctggcggg ccgagcgaca tcatcggccg gctcctggcg cagtcgatga cgcagacgct
     2761 gaagcagcag gtgatcgtcg agaacaccgc cggcgcgggc ggcactctgg ccatcaaccg
     2821 cgtcgccaag gcggcgcccg atggctacac catcctgatc tcgcatgtga atcatgccgc
     2881 gaccgcgatc ctgtatcgca agctccagta cgatccggtt gaggatttcg cgccgatcgg
     2941 cctcgccacc gacggggcga tggcgatcat cggcaagaag ggcatcgcgc ccaacacgat
     3001 tcaggagctg atggcgcata tcaaggagca gaaggacaag gtctcctacg cgcatgccgg
     3061 catcggatcg ggcgcgcatc tgtgcggcct gcagctgatg agcgcgctgc agacacagat
     3121 gacggtggtc acctatcgcg gcacgggccc tgcgctgaac gatcttgttg gcggacagat
     3181 cgacctgttg tgcgatcagg tcaccagtgc gctgccgcag attcaggccg gcacggtgaa
     3241 ggcctacgcg gtgaccacca aggagccgct gaagtccctg ccgctgccga gcatggagca
     3301 ggtgggcatc agggggttcg aggtcaatgt ctggcacggg ctttacgcgc cgaagggcac
     3361 ccccgctccg atcgtcgagc gcctggcgtc cgccctgcgc gccgcgctga aggatccgct
     3421 ggtcctgaag cgtctggacg atctctccac cacgccggtc tccgacgagc gtgcaacgcc
     3481 ggacgccctg aaaaagcacc ttgtttccga gatcgcgaag tggcggccga tcatccaggg
     3541 cgcggggcag tacgcggact gacggatatc caggggatgt ccgccggcat gccggcggct
     3601 atttccgtgc ccgatagggc gtgatccagc cgagaccggc cagcgtgccg gcgcgggggc
     3661 gatattcgca cccgacatag ccgtcatagc ccaaccggtc gagcgtcgcg aagacaaagc
     3721 cgtcgtcgag ttctccggtt ccgggctcgt gccgcagggg cacgctcgcg gtctggacat
     3781 gcccgatgat cggcatcatg gccgccagcg ccttggtcac gtcgccgtgc aggatctggc
     3841 ggtggtagat gtcgaactgc aacttcaggt tcggcagctt cagggcatca atcgttgtcg
     3901 ctgcaagggc gaaatccgtg aggaaatagc cgggattgtc gcggctgttg agcggttcga
     3961 tcagcacgtc gatgccgtgc ggaccgaaaa cctgcgccgc ttccttcagc gagtcgccga
     4021 accgagcgac acagcgcgga tcgttgcggt cggcgatgcc ggccatcagg tgcagccggc
     4081 gcgcgccggt cgcctgcgcg tagcgcagtg cgagcggcag gcttgccgac aggtccgagg
     4141 cccgctccgg caatgccgcg aaaccgcgct cgcccctggc gaagtcgccg gggtagacgt
     4201 tgaacagggc ctgggtcagc cggcagtcgg acagctttcg ggcgatgtcc tcggccggga
     4261 gctgcgccat gg
//
LOCUS       DAOMXN010000218         4236 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_11274_length_4236_cov_10.941880, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000218 DAOMXN010000000
VERSION     DAOMXN010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11274_length_4236_cov_10.941880"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="PK812_12265"
     CDS             complement(<1..938)
                     /locus_tag="PK812_12265"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRJ70368.1"
                     /translation="MVLPFAEASVADVLAFCAWLSIALVHPAVMVLALLHPWTVRRLA
                     RQREDQPPVSVLLPVSAADHDVAGCLDRVVRLSYPRFEVLVCSDSVDAPAFRLIKDHA
                     QRIEGADLRFFVARERIGRNPKINNLVPALAASNYETQLIKDANVAFEVGDLARLVRY
                     LGSGTGLVCSIPVGVNPRSLAADVEQSMMNGRDAPYTLAASVLGMNLGYGKVMLFRKA
                     DLVQAGGLGILADHFGDDHALSVAMGRLGLATVYTDGFVAQPLENRSWRTVLDRQLRW
                     LVIRRNQAFPAFFLEPFMTWPAAVASAWLAAPLLGWT"
     gene            1209..2177
                     /locus_tag="PK812_12270"
     CDS             1209..2177
                     /locus_tag="PK812_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRJ70369.1"
                     /translation="MTAKTILVTGGSGFLGSHLCDRLIREGHDVVCVDNYFTGSKRNV
                     QHLIAHPRFEVMRHDVTMPLYVEVDAIYNLACPASPIHYQYDPVQTTKASVHGAINML
                     GLAKRLKCRIFQASTSEVYGDPTIHPQTEDYWGNVNPIGPRSCYDEGKRCAETLFFDY
                     HRQHRLEIKVARIFNTYGPRMHPNDGRVVSNFIVQALRGDPITLYGDGSQTRSFCYVD
                     DLIEGMVRLMNTPAEVTGPVNLGNPAEFTIRELAEQVIDLTGSASRLDFRPLPQDDPR
                     QRRPDIALARRLLDDWQPGVGLREGLTKTIAYFDRLLADRPGDASP"
     gene            2174..3157
                     /gene="galE"
                     /locus_tag="PK812_12275"
     CDS             2174..3157
                     /gene="galE"
                     /locus_tag="PK812_12275"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HRJ70370.1"
                     /translation="MTAPAILVTGGAGYIGAHTCKALARAGYRPVTYDDLSAGHREAV
                     RWGPLEVGDIADRARLGAVLARWSPKAVLHLAAHAYVGESVEHPGKYYRNNVAGSLSL
                     LEALRDHAMTRIVFSSSCATYGMPDMLPITEEMEQRPISPYGASKLMTERMLADFGAA
                     HGLHAIALRYFNAAGADPDGEIGEDHTPETHLIPLVITAAAGRRPAVQVFGDDYPTPD
                     GTCIRDYVHVSDLADAHVRALQALERGQGPVACNLGTGRGWSVRDIIGAVERVSGRPV
                     PVVMGARRPGDPAVLIASAARAASVLGWKPRWPNLDDIVRTAWAWHTRHGK"
     gene            complement(3212..>4236)
                     /locus_tag="PK812_12280"
     CDS             complement(3212..>4236)
                     /locus_tag="PK812_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015343352.1"
                     /note="has catalase and peroxidase activities; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="catalase-peroxidase"
                     /protein_id="HRJ70371.1"
                     /translation="NPDQFADAFARAWFKLTHRDMGPRVRYLGPEVPSEELIWQDPVP
                     AADHELVGASDIAVLKQNILATGLSVSDLVSVAWASASTFRGSDKRGGANGARIRLAP
                     QKDWAANEPARLSKVLSALEGVQNAFNAKSGKKKISLADLIVLAGGAAIEAAAKAGGH
                     DVSVPFTPGRTDATQAQTDVESFSVLEPQADGFRNYQKKAFSVSPEALLVDRAQLLTL
                     SAPEMTVLVGGLRVLGANHGGSQHGVLTKRVGVLSNDFFVNLVDMATEWKPVGASGEM
                     FEGSDRTNGQKKWTATRVDLVFGSNSQLRALAEVYAQDDAKERFVNDFVAAWTKVMNA
                     DRFDLA"
BASE COUNT          784 a         1435 c         1321 g          696 t
ORIGIN      
        1 gtccagccga gcagcggcgc cgcgagccat gcgctcgcca ccgccgccgg ccaggtcatg
       61 aagggttcga gaaagaaggc tggaaaggcc tggttccggc ggatgaccag ccaccgcaac
      121 tgtcgatcca gaaccgtccg ccaggagcgg ttttcgagcg gctgggccac gaacccgtcg
      181 gtgtaaacgg ttgccaggcc caggcgcccc atcgccacgg agagggcatg atcgtcaccg
      241 aagtgatcgg ccagaatgcc gagtcccccg gcctggacca gatccgcctt tctgaacagc
      301 atcaccttgc cgtagcccag gttcatgccc agtacggaag ccgccagcgt gtagggtgcg
      361 tcgcgaccgt tcatcatcga ctgctcgacg tccgccgcaa gactgcgcgg gttcacgcca
      421 acggggatgg agcagacgag gccagtgccg gagccaaggt agcgcaccag acgcgcgaga
      481 tcgccgactt cgaacgcaac attcgcatcc ttgatcagct gcgtttcata gttcgaggcc
      541 gcgagcgccg gaacgaggtt gttgatcttg gggttacgcc cgatgcgctc ccgggcgacg
      601 aagaagcgca gatcggctcc ctcgatccgc tgcgcatgat ccttgatcag gcgaaaggcc
      661 ggcgcgtcga cgctgtcact gcacacgagg acctcgaagc gcgggtatga aagcctgacc
      721 acacggtcaa gacacccggc aacatcatga tcggcggcgc tgaccggcag cagcaccgaa
      781 accggcggct gatcctcccg ttgccgcgca agacggcgca ctgtccaggg atgcagcagg
      841 gccaggacca tcacggcggg gtgtacaagc gcaatcgaca gccatgcgca gaacgccagc
      901 acgtcggcga ctgacgcttc ggcaaatggc aaaaccatga agagcgcgcc ctatgccggt
      961 ccttcggcaa ggcccggacc ctgatccggc cgcagacctc acgcgaagtc tattcaacgg
     1021 gcgctgtcgc ggcaaggcag gccgtgtcac cctgcccgcg acttcgagcg ccctccgtcg
     1081 gatcgcggtt tgatctgtct gatagcgcgc gccgcgcttt gctgcatacg aggtccggtg
     1141 atgttgggac agcgggacac ggccggtcag agcgggcagc gttccagcgc tggaaatgaa
     1201 ggcaaaaaat gacggccaag acaattctcg taacgggcgg cagcggtttc ctgggctcgc
     1261 acctgtgtga tcgactgatc cgcgaggggc atgacgtcgt ctgcgtcgac aattacttca
     1321 ccggctcgaa gcgcaacgtt cagcacctga tcgcgcatcc gcgcttcgag gtgatgcgac
     1381 acgatgtcac gatgccgctg tacgtcgaag ttgatgcgat ctacaacctc gcctgcccgg
     1441 cttccccgat ccactaccag tacgatccgg tgcagacgac caaggccagc gtacatggcg
     1501 cgatcaacat gctcggtctt gccaagcgcc tgaagtgccg catcttccag gcctcgacct
     1561 cagaggtcta cggcgatccc accatccatc cccagaccga ggactactgg ggcaacgtca
     1621 atcccatcgg cccgcgatcc tgctacgatg aaggcaagcg ctgcgcggaa accctgttct
     1681 tcgactatca tcgccagcac cggctggaga tcaaggtcgc gcgcatcttc aacacctatg
     1741 ggccgaggat gcacccgaat gacggacgcg tggtatccaa tttcatcgtg caggcattgc
     1801 gcggtgatcc tatcacgctc tatggcgacg gcagccagac gcgctcgttt tgctatgtcg
     1861 acgatctcat cgagggcatg gtccggctga tgaacacccc cgccgaggtg accggccccg
     1921 tcaatctcgg caatccggcg gaattcacaa tccgcgaact ggccgaacag gttatcgatc
     1981 tgaccgggtc cgcttcccgc ctcgacttcc gtcccctgcc gcaggacgat ccccgccaac
     2041 gccggcccga catcgcgctt gcgcggcggc tgctggacga ctggcagccc ggcgtcggcc
     2101 tgcgcgaggg cctgacgaag accatcgcct atttcgatcg gctgctcgca gaccggcccg
     2161 gtgacgcttc gccatgaccg ctcctgccat actggtcacg ggcggcgcgg gttacatcgg
     2221 tgcccacacc tgcaaggcgt tggcgcgggc cggttatcgt ccggtgacct atgacgatct
     2281 cagcgccggg catcgcgagg ccgtccgatg ggggccgctc gaagtcggcg atattgcgga
     2341 tcgcgcccga cttggggcgg tgctggcgcg ctggtcgccg aaggcggtgc tgcacttggc
     2401 cgcccacgcc tatgtgggcg agtccgtcga acatccgggc aagtactacc gcaacaacgt
     2461 cgcgggttcc ctgtcgctgc ttgaagcgct tcgcgatcac gccatgaccc ggatcgtctt
     2521 ttccagttcc tgcgctacct atggcatgcc ggacatgctg ccgatcaccg aagagatgga
     2581 gcagcggccg atcagccctt acggggcgag caagctgatg accgagcgga tgcttgccga
     2641 cttcggcgcg gcgcacggcc tgcacgccat cgcactgcgc tacttcaatg cagcgggtgc
     2701 cgatccggac ggcgagattg gcgaagacca cactccggag acccacctta tccccctggt
     2761 gatcacggcg gcggccggcc gacgaccggc cgtgcaggtg ttcggcgacg actacccgac
     2821 gccggacggc acctgcatcc gcgactacgt ccatgtcagc gatcttgccg atgcccatgt
     2881 ccgcgcactg caggccctgg aacggggaca gggcccagtc gcctgcaatc tgggcaccgg
     2941 tcgcggctgg tccgtgcgcg acatcatcgg tgcggtcgaa cgggtaagcg gcagaccggt
     3001 tccggtcgtc atgggcgcgc ggcgtcccgg cgatcccgcc gtgctgattg cctcggccgc
     3061 aagggcggct tccgttctcg gctggaagcc acgatggccc aacctcgatg acatcgtgcg
     3121 cacggcctgg gcctggcaca cccggcacgg caaatgaaga aagggcgcgg ccccggaaag
     3181 agccgcgccc tctgttacga acgtgggcct gtcacgccag atcgaagcga tccgcgttca
     3241 tcaccttggt ccaggccgcc acgaagtcgt tgacgaaccg ctccttcgca tcgtcctggg
     3301 catagacctc ggccagagcg cgcagctggg agttcgaacc gaacaccaga tcaacccggg
     3361 tcgccgtcca cttcttctgc ccgttggtcc ggtcgctgcc ctcaaacatc tctcccgaag
     3421 cgccgaccgg cttccactcc gtggccatgt ccaccaggtt gacgaaaaag tcgttcgaca
     3481 gcacgccgac gcgcttggtg agcacgccgt gctgcgagcc gccgtggttc gcgcccagca
     3541 cgcgaaggcc gccgaccagc accgtcatct caggcgccga aagcgtcaac agctgcgcgc
     3601 gatccaccag caaggcctcc ggagaaacgc tgaaagcctt cttctggtaa ttgcggaagc
     3661 cgtcggcttg cggctccagc acagagaagg attcgacatc ggtctgcgcc tgcgtggcat
     3721 ccgtgcggcc cggcgtgaac ggaaccgaca cgtcgtgtcc gcccgccttc gccgccgcct
     3781 cgatagcggc gccgccggcc agaacgatga gatccgcgag agagatcttc ttcttgccgg
     3841 acttggcgtt gaaggcgttc tgcacaccct ccagcgcaga cagcaccttg ctcaggcgcg
     3901 ccggttcgtt ggccgcccag tccttctgcg gggcgaggcg aatgcgcgcg ccattggcgc
     3961 cgccgcgctt gtcggagccg cggaatgtcg aggcagacgc ccaggcgacc gacaccagat
     4021 ccgacaccga aagaccggta gccaggatgt tctgcttcag gaccgcgata tcactcgctc
     4081 cgaccagttc atgatctgcc gccgggacag gatcctgcca gatcaactcc tcggacggca
     4141 cttccggacc gagataccgc acgcgcgggc ccatgtcgcg atgggtcagc ttgaaccagg
     4201 cgcgggcgaa agcatccgcg aactggtccg ggttct
//
LOCUS       DAOMXN010000219         4230 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_11302_length_4230_cov_8.699880, whole genome shotgun sequence.
ACCESSION   DAOMXN010000219 DAOMXN010000000
VERSION     DAOMXN010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11302_length_4230_cov_8.699880"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..993)
                     /gene="fabI"
                     /locus_tag="PK812_12285"
     CDS             complement(172..993)
                     /gene="fabI"
                     /locus_tag="PK812_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HRJ70372.1"
                     /translation="MTGASGILAGKRGLVMGVANNRSIAWGIARACRAHGAELAFTYQ
                     GDALKKRVEPLAAELDAIVVGHCDVTDAASIDAVFAEVEKHWGKLDFVVHCIAFSDKD
                     ELSGRYIETSEGNFTKSLLISCYSFTAIAQRAEKLMPEGGSLLTLTYYGAEKWMPHYN
                     VMGVAKAALEASVRYLAADLGPKNIRVNAISAGPIKTLAASGIGDFRYILKWNEYNSP
                     MRRTVTIEEVGDVGAYFCSDMARGVTGEIHHVDAGYHIVGMKNPDAPDLSLDRDT"
     gene            complement(1078..1989)
                     /locus_tag="PK812_12290"
     CDS             complement(1078..1989)
                     /locus_tag="PK812_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010683787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HRJ70373.1"
                     /translation="MRDPYTVLGVARGASEAEVKSAFRKLAKQYHPDRNSGDTRAKER
                     FAEISNAYEILGDKDKRAKFDRGEIDAEGKQKFHGFEGFSGGPFGGGAENFHFNFSTG
                     GGPGQRGGPSEDVLGDILRGFSGMGSGRGSRGGHGIPPGRDVTGEVAVSLEELATGTT
                     KRVLLPGGRQLDVKVPAWIGEGKTIRLSGQGENAVPGGPRGDVLLSVRYQPHPRFTVD
                     GADLRVRVAVPLADAVLGGSIRIPTLGGEVEMTLPAGASGGRSFRLRGKGFHGAGGAR
                     GDLIATLDIVLPEGDEELAALMRRRRG"
     gene            complement(2081..2515)
                     /locus_tag="PK812_12295"
     CDS             complement(2081..2515)
                     /locus_tag="PK812_12295"
                     /inference="COORDINATES: protein motif:HMM:NF028308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RT0821/Lpp0805 family surface protein"
                     /protein_id="HRJ70374.1"
                     /translation="MRPSTFLSAVAVLVLPLLAGCSLTMPIGSLVNEDEVTGSLPAQP
                     APMLSPKLDGEDQRRAMAALALAVDPQGNGAPVQWDNPVSRRKGGFVASGALFVKDDR
                     VCRPFSATLVLDGTDSAVHGIACRLGPNDWMLREVKPGAGPG"
     gene            2632..3234
                     /gene="pdxH"
                     /locus_tag="PK812_12300"
     CDS             2632..3234
                     /gene="pdxH"
                     /locus_tag="PK812_12300"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HRJ70375.1"
                     /translation="MSDDFTLAEDPFSLFSKWLGEASRSEPNDPNAMALASVDENGLP
                     NVRMVLMKEVDARGFVFYTNFESAKGRELLGQQKAAAVFHWKSLRRQVRVRGAVEQVT
                     AAEADIYFASRPRDARIGAWASQQSRPLESRFALEKQVVYYGARFVVGEVPRPPHWSG
                     FRIVPVEIEFWHDRPFRLHDRSVFRRADASAAWTRTRLYP"
     gene            3247..3990
                     /locus_tag="PK812_12305"
     CDS             3247..3990
                     /locus_tag="PK812_12305"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRJ70376.1"
                     /translation="MSSNAERRTLLLTGASRGIGHATVKRFSSAGWRVIACSRQPFPE
                     DCPWEAGRDDHVQVDLADINDTMRAIDEIHRRLGDGKLHALVNNAGVSPKGPGGSRLG
                     AIDTAVRDWNHVFQVNFFAPILLARGLINELKATRGSVVNVTSIAGSRVHPFAGAAYA
                     TSKAALQALTREMAADFGPFGIRVNAISPGEIDTSILSPGTEKIVETLPMRRLGTPEE
                     VAKAIYFLCTEQSSYVTGAELHINGGQHV"
     gene            3987..>4230
                     /locus_tag="PK812_12310"
     CDS             3987..>4230
                     /locus_tag="PK812_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRJ70377.1"
                     /translation="MSRLYPERPFLAASVAVFREGRVLIAERASGAGIGSWSLPGGMV
                     ETGERLGEAALRELFEETGVRAELAGFVDTVEIIGHD"
BASE COUNT          718 a         1380 c         1379 g          753 t
ORIGIN      
        1 accctgcatt ccggctgcgg gcggggccgg gagcggcgcc ggagcctgtg tctgggcggc
       61 ggcgccgagg ctggttccaa taagactggt tccaatcagc atcaggatcg cggcaacacg
      121 catgggtcaa ccctcggctt gttctcggcg cctttcttgc cgtgattcgc gtcaggtgtc
      181 gcgatccagg ctcagatccg gtgcatcggg attcttcatg ccgacgatat gatagccggc
      241 atcgacatga tgaatttcgc cggtcactcc gcgcgccata tcggagcaga aataggcacc
      301 gacgtcgccc acctcctcga tcgtcaccgt gcggcgcatc ggcgaattgt attcgttcca
      361 cttcagaatg tagcggaaat caccgatgcc ggaggcggcg agcgtcttga tcggaccggc
      421 cgagatggca ttgacgcgga tgttcttcgg gccgagatcg gcagcgagat agcgcacgct
      481 ggcttccagc gccgccttgg ccacgcccat gacgttataa tgcggcatcc atttctcggc
      541 gccgtaatag gtcagcgtca gcagcgagcc accctcgggc atcagctttt cggcgcgctg
      601 ggcgatggcg gtaaaggaat agcaggaaat cagcagcgac ttggtgaaat tgccttcgct
      661 ggtttcgatg tagcggcccg aaagctcgtc cttgtcggag aaggcgatgc aatggacgac
      721 aaaatcgagc ttgccccagt gcttctccac ctcggcgaag acagcatcga tgctggcggc
      781 atcggtcacg tcgcaatggc cgacgacaat ggcgtcgagt tctgcggcga gcggctcgac
      841 tcgcttcttg agtgcatcgc cctgataggt gaaggccagt tcggcgccgt gcgcgcggca
      901 ggcgcgcgcg attccccagg cgatcgaccg gttgttggcc acgcccataa ccagaccgcg
      961 cttgccggcc agaatgccgc tcgcccctgt catgctctgc tcccgctcgg atccgcttgt
     1021 gtttgggcgc atacctaacg attgcaacga aaggcgcaag cgggaaaagc cggaaaatca
     1081 gccgcgccgg cgtcgcatca gcgctgccag ttcctcgtcg ccctcgggca acacgatgtc
     1141 gagcgtggcg atgagatcgc cccgcgcccc gcccgcgccg tgaaagccct tgccgcgaag
     1201 gcggaagctg cggccgccgc tggcgccggc tggcagggtc atctcgacct cgcctcccaa
     1261 cgtcggaatc cggatgctgc cgcccagcac cgcatcggcg agcggaaccg cgacccgcac
     1321 cctgaggtcg gcgccatcga ccgtgaatcg cgggtgcggc tggtagcgca cgctcagcag
     1381 gacatcgcca cgcggcccgc cgggaacggc attttcgccc tggccggaaa gccggatggt
     1441 cttgccctcg ccgatccatg ccgggacctt gacgtcgagc tggcgcccgc cgggcagcaa
     1501 gacacgcttg gtggtgcccg tcgccagctc ttcgaggctg accgccacct cgccggtcac
     1561 atcgcgaccg gggggtatgc catgccctcc gcgggagcca cgaccgctcc ccatgccgga
     1621 aaagccgcgc agaatgtcgc cgagaacatc ctcggacgga ccgccccgct ggccggggcc
     1681 gccgccggtg ctgaaattga aatgaaagtt ctccgcgccg ccgccgaatg gtccgccgga
     1741 aaagccctcg aacccgtgga atttctgctt gccctcggca tcgatctcgc cgcgatcgaa
     1801 tttggcccgc ttgtccttgt cgccgaggat ctcataggca ttgctgatct cggcgaagcg
     1861 ctccttggcg cgtgtatcgc cgctgttgcg atcgggatga tactgcttgg cgagcttgcg
     1921 gaaggccgat ttgacctcgg cctcgctggc gccccgggct acgcccagca cggtataggg
     1981 atcacgcatc atcgccctcc ttgagaatgg cgaggatcag ggtggaagtt gtcccggatg
     2041 tggggagccg accctgctat tgcaactgcg aaggcttgtt tcagcccggt ccggcgccgg
     2101 gtttgacctc gcgcagcatc cagtcgttcg gaccgagccg acaagcgatg ccgtggaccg
     2161 cgctgtcggt gccatcgagc accagcgttg ccgaaaacgg tcggcagaca cgatcatcct
     2221 tgacgaacag ggcgccggag gcgacgaacc cgcccttgcg ccgggagacc ggattgtccc
     2281 attgcaccgg ggcgccgttg ccctgcggat cgaccgccag cgccagtgcg gccatggcgc
     2341 ggcgctgatc ctcgccgtcg agcttgggcg agagcatggg agcgggctgg gccggaagcg
     2401 agccggtcac ctcgtcctcg ttgaccagcg agccgatcgg catggtcagg ctgcacccgg
     2461 caagcagcgg cagcaccagc acggcgacag ccgacaggaa ggtggatggg cgcattccgt
     2521 tatcgcgcaa acgcacgggg aggctataag acagacacga gtattcgccg caacgcatga
     2581 tcaacacccc agccagggtc ccccaccggt catttcacgg gaaatcagtt aatgtctgat
     2641 gacttcacgc tcgccgaaga cccattttcc ctgttttcga agtggcttgg cgaggccagc
     2701 cgcagcgagc cgaacgatcc caatgcgatg gcgctggcca gcgtcgacga aaacggcctg
     2761 cccaacgtgc gcatggtgct gatgaaggag gtcgatgcgc ggggatttgt tttctacacc
     2821 aatttcgaaa gcgccaaggg ccgcgaattg ctcggccagc agaaggcagc ggcggtgttt
     2881 cactggaaga gcctgcggcg tcaggtgcgc gtccggggtg cggtcgagca ggtcacggcg
     2941 gccgaggccg atatctattt cgcctcccgc ccgcgcgatg cccgcatcgg cgcctgggct
     3001 tcgcagcagt cccgcccgct ggaaagtcgc tttgcgcttg aaaagcaggt tgtctactat
     3061 ggcgcgcgat ttgttgtcgg cgaggtgccg cgtccgcccc attggagcgg tttccgcatc
     3121 gtgccggtgg agatcgagtt ctggcacgac aggcccttcc gtctgcatga ccgctcggtg
     3181 tttcgccgag ccgatgcgtc ggctgcctgg acgcgcacgc gcctgtatcc ttgaggagtc
     3241 gccctgatgt cttccaacgc cgaacgccgc accctgttgc tgaccggcgc ctcgcgcggc
     3301 atcggccatg caaccgtcaa gcgcttctcc tctgccggct ggcgggtgat cgcctgctcg
     3361 cgccagccgt ttccggagga ttgcccctgg gaggccgggc gcgacgatca tgttcaggtc
     3421 gatctggccg acatcaacga caccatgcgc gctatcgacg agatccatcg ccggctcggc
     3481 gacggcaagc tgcatgcgct ggtcaacaac gccggggtct cgcccaaggg gccgggcggc
     3541 agccggctcg gggccatcga cacggcggtt cgcgactgga accatgtgtt ccaggtcaat
     3601 ttcttcgcgc cgatcctgct ggcgcgcgga ttgatcaacg agctcaaggc aacgcgcggc
     3661 tcggtggtca atgtcacctc gattgccggt tcgcgcgtgc atcctttcgc cggcgccgct
     3721 tatgccacat cgaaggcggc gttgcaggca ctgacccgcg agatggccgc cgatttcggc
     3781 ccctttggca tccgcgtcaa cgccatttcc cccggcgaga tagacacatc gattctctct
     3841 cccggcaccg agaagattgt cgagaccctg ccgatgcgcc ggcttggcac gcccgaggag
     3901 gtcgccaagg cgatctactt cctgtgcacc gagcagtcgt cctacgtcac cggcgcggaa
     3961 ctgcacatca acggtggcca gcacgtatga gccggctcta tcccgaacgg ccgtttctgg
     4021 cggcgtcggt cgcggtgttc cgcgaagggc gcgtgctgat cgccgagcgg gcgagcgggg
     4081 cagggatcgg cagctggtcg ctgccgggcg gcatggtcga gaccggcgaa cgcctcggcg
     4141 aggcggcctt gcgcgaattg tttgaggaaa ccggcgtgcg ggctgaactt gccggcttcg
     4201 tcgatacggt cgagatcatc ggccacgatg
//
LOCUS       DAOMXN010000220         4192 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_11455_length_4192_cov_10.821126, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000220 DAOMXN010000000
VERSION     DAOMXN010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11455_length_4192_cov_10.821126"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="PK812_12315"
     CDS             <1..574
                     /locus_tag="PK812_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70378.1"
                     /translation="ESRRRKLSPEWELPRAPRRTSRPALDQKNASAPSLPTTRIAYVV
                     RNQNLDQLLREVGQLANVRVVPGPGVRGAVRERRLEGPFNTVLDRMARDFNLFWFADG
                     GTIYVDPADEQKSKIFRVKGSSPEHIDRALEAAGLGRHRDRIQAVGSDGLVRASGPEN
                     FLRSVESILTGVAGTDTGTVQVIRFGQRSQ"
     gene            882..2435
                     /locus_tag="PK812_12320"
     CDS             882..2435
                     /locus_tag="PK812_12320"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019906148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HRJ70379.1"
                     /translation="MSTTKDRVLIFDTTLRDGEQCPGATMTFEEKLEVADMLDELGVD
                     IIEAGFPIASEGDFEAVSAIAKRVKNATVAGLARAGANDIDRAGEAVKHARRGRIHTF
                     ISTSPVHMKWKLQKTPDEVLGLVISSVTRARNLVEDVEWSAEDGTRTEMDFLCRCVEA
                     AIKAGAGTINIPDTVGYTTPEEYFDLFRTVRERVPNSDKAIFSVHCHNDLGMAVANSL
                     AGVKAGARQIECTINGIGERAGNAALEEIVMAIKTREDVLPFETGIQSTGLNHASRLV
                     AAATSFPVQYNKAIVGRNAFAHESGIHQDGMLKHTQTYEIMTPESVGVSKTSLVMGKH
                     SGRNAFRARLKDLGYQLGDNQLEDAFVRFKALADRKKHVYDEDIEALVDEGIATAQDR
                     VRVLSLLVVAGTNGPQSAVLTLDIDGQHHTRQATGNGPVDATFNAIHALIPHEAKLAL
                     YQVHAVTAGTDAQAEVSVRLEDGKTGRLVTGKAADPDTLVASAKAYIAALNKLTAREA
                     GLHAQHATV"
     gene            2537..2956
                     /locus_tag="PK812_12325"
     CDS             2537..2956
                     /locus_tag="PK812_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor 2"
                     /protein_id="HRJ70380.1"
                     /translation="MTLKGLALASVVLVAGCATVTRGTTSQVQITSEPMGAQVRTSIG
                     HVCTSPCTVTVDRKSEFFVTFSMDGYQEVTVPVRTQVATAGAVGVAGNVIVGGIVGGA
                     VDVATGAGLEHVPNPVHAILQPALSRPAGPRPGVPRR"
     gene            complement(2987..3394)
                     /locus_tag="PK812_12330"
     CDS             complement(2987..3394)
                     /locus_tag="PK812_12330"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRJ70381.1"
                     /translation="MSLVLDCSATLAWLHDDERAPQVQRIFDHVVESGACVPGLWHLE
                     VANALTMSVRRKRITPEQRDEFLAQLSNLDIQTDVHTAQEAWSATLRLADRHGLTVYD
                     AAYLELAERRALPLATLDDDLRKAAEAAGVVVV"
     gene            complement(3391..3645)
                     /locus_tag="PK812_12335"
     CDS             complement(3391..3645)
                     /locus_tag="PK812_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HRJ70382.1"
                     /translation="MRQIGAFEAKNTLGTLLDLVMQGEEIVITRHGKPVARLIREPGR
                     DEKRERARQAAEALEALAKGAKLGRFDWQDWRSLRDEGRR"
     gene            3888..3963
                     /locus_tag="PK812_12340"
     tRNA            3888..3963
                     /locus_tag="PK812_12340"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3921..3923,aa:Lys,seq:ctt)
BASE COUNT          724 a         1393 c         1367 g          708 t
ORIGIN      
        1 cgagagccgg cgccgcaaac tctcgcccga gtgggagctg ccgcgcgcgc cgcgacgcac
       61 cagccgtccc gcgctggatc agaagaacgc gagcgcaccg tcgctgccga ccacgcgcat
      121 cgcctatgtc gtgcgcaatc agaatctcga ccagctcctg cgcgaagtcg gccagttggc
      181 gaatgtccgg gtcgtgcccg gtccaggcgt gcgcggagcc gtgcgcgaga ggcgcctcga
      241 agggccgttc aacacggtgc tcgacaggat ggcgcgcgac ttcaacctgt tctggttcgc
      301 cgatggcggc accatctatg tcgatccggc ggacgagcag aagtccaaga tcttcagggt
      361 caaaggctcc tcgcccgagc acatcgacag ggcgctggag gccgctggcc tcggccgcca
      421 tcgcgaccgc atccaggcgg ttggcagcga cggtctcgtc agggcgagcg gtccggagaa
      481 tttcctgcgc tcggtcgaga gcatcctgac cggggtcgcc ggcacggata ccggcaccgt
      541 gcaggtcatc cggttcgggc agcgctcgca gtaaggccgc gtgaccgaac ggcggcgact
      601 tgcgctccgt cgcgaatttc actatccgtc ttggcattga gcaggagctt cgcgccgtga
      661 cggcagcgtt ttgtcagaca ccgacgggaa ccggcagcca ggcgcgctgc gggatgccgg
      721 cgtctgcgct tcttctcagc ctgcttctta tgggcccctg agggccgtcc gggcgcgctt
      781 taagcggttg gcccgggtct tcaggggagc gcagcaagtc cgatcccgac attcagcctg
      841 cgacggcaca ccggcaggca caaagccaag gaaccgcttt catgagcacg accaaagacc
      901 gcgtcctgat cttcgacacc accctgcgcg acggcgagca atgccccggc gccaccatga
      961 ccttcgagga gaagctcgaa gtcgccgaca tgttggatga actcggcgtc gacatcatcg
     1021 aggccggctt tcccatcgct tcggaaggcg acttcgaagc cgtcagcgcc atcgcgaaac
     1081 gggtgaagaa cgccaccgtc gccggcctcg cccgtgccgg cgcgaacgac atagaccggg
     1141 ccggcgaggc ggtgaagcac gcccggcgcg gccgcatcca cactttcatc tccacctcgc
     1201 ccgtccacat gaagtggaag ctgcagaaga cgccggacga ggtgctgggc ctcgtcatct
     1261 cctccgtcac gcgcgcgcgt aacctggtgg aggatgtgga atggtcggcc gaggacggca
     1321 cccgcaccga gatggacttc ctgtgtcgct gcgtcgaggc ggcgatcaag gctggcgccg
     1381 gcaccatcaa catcccggac accgtgggct acaccacgcc ggaggagtat ttcgacctgt
     1441 tccgtaccgt gcgcgagcgc gtgcccaact cggacaaggc gatcttctcg gtccattgcc
     1501 ataacgacct cggcatggcg gtcgccaatt cgctggccgg cgtgaaggcc ggggcgcgcc
     1561 agatcgagtg caccatcaac ggcatcggcg agcgggcagg caatgccgcg ctggaagaga
     1621 tcgtgatggc gatcaagacc cgcgaggacg tgctgccgtt cgagaccggg atccagtcga
     1681 ccggactcaa tcacgcctcc cggctggtgg ccgccgccac ctcctttccg gtgcagtaca
     1741 acaaggcgat cgtcggccgg aacgccttcg cccacgagag cggcatccat caggacggca
     1801 tgctgaagca tacccagacc tacgagatca tgacgccgga atcggtcggc gtgtccaaga
     1861 cctcgctggt gatgggcaag cactcgggtc gcaatgcctt ccgcgccagg ctgaaggacc
     1921 tgggctacca gctcggcgac aaccagctgg aggatgcgtt cgtgcggttc aaggcgctcg
     1981 ctgaccgcaa gaagcacgtc tatgacgagg acattgaggc gctggtcgac gagggcatcg
     2041 ccaccgcgca ggaccgggtc cgcgtgctgt cgctgctggt cgtggccggc accaacggcc
     2101 cgcagtcggc ggttctgacg ctcgacatcg acggccagca ccacacccgt caggccaccg
     2161 gcaacggtcc ggtggatgcg accttcaatg ccatccatgc gttgattccg catgaggcga
     2221 agctggcgct gtatcaggtc catgccgtga ccgcgggcac ggacgcgcag gcggaggtgt
     2281 cggtgcggct ggaggacggc aagaccggcc gcctcgtcac cggcaaggcg gcggacccgg
     2341 atacgctcgt cgcctcggcc aaggcctata tcgcggcgct gaacaagctg acggcgcggg
     2401 aagccggcct gcacgcgcag cacgcgacgg tgtgaggttt tttcctcaca tccccgtcaa
     2461 tcgggagccc ggtccttgcg accgggcttt gcctttgcac gataggtgcg gggcgctgaa
     2521 caacgggatc gattcgatga cactgaaggg gctcgcattg gcgagcgtgg tgctggtggc
     2581 cggctgcgcg acggtgacgc gcggaacgac aagccaggtg cagatcacct ccgagccgat
     2641 gggcgcgcaa gtgcgcacct cgattggtca cgtctgcacc tcgccttgca cggtgacggt
     2701 cgaccgcaag agcgagttct tcgtcacctt ctcgatggac ggttatcagg aagtgacggt
     2761 gccggtccgc acacaggtcg ccacggctgg cgcggtcggc gtcgctggca acgtcatcgt
     2821 tggcggcatc gtcggcggcg cagtggatgt ggccactggc gcaggccttg aacatgtgcc
     2881 caatcccgtg cacgccatcc ttcagcccgc gctctcccgg ccggccgggc cacgtccggg
     2941 cgttccccgg agataggcga aagcccctcg cgcgccgtct tcgccttcac acgacgacaa
     3001 cccccgccgc ttcggctgcc ttcctcagat cgtcgtccag cgtcgccagc ggcagggcgc
     3061 ggcgttccgc gagttccaga taggccgcat cgtagacggt gaggccgtgg cggtccgcaa
     3121 ggcgcagcgt cgcggaccat gcttcctgtg ccgtgtggac atcggtctgg atgtcgaggt
     3181 tgctcagttg cgcgagaaat tcatcgcgct gctccggcgt aatgcgtttt cgtcgcaccg
     3241 acatcgtcaa tgcattggcg acttccagat gccagaggcc ggggacgcag gcgccacttt
     3301 ccacaacatg gtcgaaaatg cgttgcacct gcggcgcccg ctcatcgtcg tgaagccagg
     3361 ccagcgttgc ggagcagtcg agaacaagac tcaccgccgc ccctcgtcac ggagggatct
     3421 ccagtcctgc caatcgaacc ggcccagctt tgcccctttc gccagcgcct ccagcgcttc
     3481 cgccgcctgc cgggcgcgtt cgcgcttttc gtcgcggccc ggctcgcgga taaggcgcgc
     3541 gaccggcttg ccgtggcggg tgatgacgat ttcctcgccc tgcatcacaa gatccagcag
     3601 cgtgccgagg gtattcttgg cttcgaaggc cccgatctgg cgcatggcac tctcctgcgg
     3661 ctgcggatca tccagcttaa ttggctgatc cagcttaatt agcttaacca gcttaaatta
     3721 agacgcctgt gtcggcaagg cacggcttgg ccgcaccgaa atcgcccgcg aatttccccc
     3781 atagggcggg tagacaggcc gggcgggctt tgctagagag cgcgcctgtc ggggcgcttc
     3841 gcgtgcggtg cgccccgttt gatttgacgg cggcacgccg gcgtcatggg tgtgtagctc
     3901 agttggtaga gcagctgact cttaatcagc gggtccaagg ttcgaatcct tgcacaccca
     3961 ccaaatccct ctctgacctc acgtgatgat cccgaaagtc cgggaccggt ccgcgctttg
     4021 gatcgctgca tcgccccatc gccgaaaatg gtgttggaac aggcggcatc tgctgagata
     4081 agctggcgct cccttggcag gcagcccgca tgcatcagca ccgcagcgcg cccgatttca
     4141 ccgaccgtcc ggcgcgccgt gtaagcacgg tgctgacgct gctggctgcg gc
//
LOCUS       DAOMXN010000221         4125 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_11710_length_4125_cov_10.059214, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000221 DAOMXN010000000
VERSION     DAOMXN010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11710_length_4125_cov_10.059214"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            506..1717
                     /gene="mnmA"
                     /locus_tag="PK812_12345"
     CDS             506..1717
                     /gene="mnmA"
                     /locus_tag="PK812_12345"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HRJ70383.1"
                     /translation="MNSLDLPKPPAETRVVVAMSGGVDSSVVAVQLASEGYDVVGVTL
                     QLYDHAEATHRKGACCAGQDIQDARRVAEHLGIPHYVLDYESRFRDAVIDAFAASYAA
                     GETPVPCIECNRTVKFRDLLAVARDLGADALATGHYIETRQLADGRRAMFRPADQSRD
                     QSYFLYATTQDQLDFLRFPLGRLTKAETREIARGAGIAVADKPDSQDICFVPQGRYDA
                     IVEKLRPEAARPGDIVHLDGRVLGRHQGVHLYTIGQRRGLGLATGEPLHVIRLDAAAN
                     RVMVGPRSALSASRLALRDFNWLGDGVPDAWGQSGFEAMVRVRSTRKPCPALLHWRGG
                     RVEVEISGGEEGIAPGQACVVYGHEASGARVFGGGTIDRVLSAEGETCVPAPAGPAGR
                     PAETSTTEHMS"
     gene            1714..2355
                     /locus_tag="PK812_12350"
     CDS             1714..2355
                     /locus_tag="PK812_12350"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRJ70384.1"
                     /translation="MSDYDLATNVRAYERWAPFYDKVYTRLLSQARSASVRAAVACGP
                     EILEVGLGTGLSLPEFPADVRVSGVDLSSHMLAKAQEKIERQGLTCVRGLAVMDACNL
                     GFAPGRFDAVSAQFVITLVPDPEQALDEFARVLKPDGEIIIANHIGAESGPMAVFESL
                     AEPLAKKLGWRPNFPLQRIRDWAGSRGFEVLSAEKVRPLGYFMVIRLKRAAKN"
     gene            2448..2524
                     /locus_tag="PK812_12355"
     tRNA            2448..2524
                     /locus_tag="PK812_12355"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2482..2484,aa:Met,seq:cat)
     gene            complement(2572..3570)
                     /locus_tag="PK812_12360"
     CDS             complement(2572..3570)
                     /locus_tag="PK812_12360"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018493417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HRJ70385.1"
                     /translation="MKAILSETVGGPETLVLREIDAPSPGPKQALIAVKAVGVNFPDA
                     LIIRDLYQFKPQRPFAPGGEIAGVVEAVGAEVKRLKPGDRVIGFCGWGGMAEKIALDE
                     ARCIPYPADIPFDVAAGLLMTYATSHHALKDRAALKPGETLLVLGAAGGVGIAAVELG
                     KAAGATVVAAVSSEEKAAVAKKHGADRTLIYPAGPFDADGRKALAAVFKDAVGPNGAN
                     VIYDPVGGDYSEAALRAIAYEGRFLVVGFPAGIPKLPLNLTLLKACQVIGVFWGDFTV
                     RRPRDNAANIMELLGMLRDGTIRPEISSRYPLERAGEAIAELAERRARGKVIVTVG"
     gene            complement(3628..>4125)
                     /locus_tag="PK812_12365"
     CDS             complement(3628..>4125)
                     /locus_tag="PK812_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRJ70386.1"
                     /translation="VAHCPCSNMRLASGIAPVKRMICEGVPVGLGVDGSASNDGGHML
                     NEARQAMLLQRVGHGPDAMSARQALELATLGGARVLGRDDIGHLSAGMSADVIAFDMR
                     KLPFAGGLHDPVAALLFCASADVAWSIINGRVVVRKGHLETLDLPVHVERHNRLARQL
                     VNRER"
BASE COUNT          652 a         1367 c         1426 g          680 t
ORIGIN      
        1 cggacgcggc ctctttctgt gccgctgccc cggcgccggc gcgctcgcgg ctaatcggtg
       61 tcgcccgtgc tgtggggcgg ccgtccggct catgggtctc acgtcctccg gccgtctgct
      121 caagcgcggc ctcgaagctt tcggggggct tgcggggttc cggccggtcc gcgcgcggcg
      181 acggggcgcg ggacaacgcc ggctcgccgg catgcgggcg aagcagggat ggtttcacga
      241 gatgtgcggc ttgcggcatg ggggcgagcg gggaacgagg gactgaccgg cgtcctgcaa
      301 gctgcttgcc ggccgtgaaa cggcgcgcga agcgctgttg aagcgacaaa ttcggcggaa
      361 atcgccggac tgctcgcgga tcccggccgg cctccggcaa aatatgccgg gtcaaagctg
      421 cctggctgga aactgccgcg aaaaaaccct atggaagcgg caagaggctc taccaccggt
      481 ttctttcgcc atcatgtccc tgcgcctgaa ctccctcgac ctgccgaagc ctccggccga
      541 gacccgtgtg gtggtggcca tgtccggagg cgtggattcc tcggttgtcg cggttcagct
      601 cgcttcggaa ggctatgatg tcgtcggggt tacgctccag ctgtacgacc atgccgaggc
      661 tacccatcgc aagggcgcct gctgcgccgg ccaggacatt caggatgcgc gccgcgtggc
      721 cgagcacctc ggcattccgc actacgttct cgactacgag agccggttcc gcgatgcggt
      781 gatcgatgcc ttcgcggcaa gctatgctgc gggcgagacg ccagttccgt gcatcgagtg
      841 caaccggacc gtgaagttca gggacctgct ggcggtggcg cgcgatctcg gcgccgatgc
      901 gctggcgacc ggccactaca tcgaaacgcg gcagctggcg gacggccggc gcgccatgtt
      961 ccgccctgcc gatcagtcgc gcgaccagag ctacttcctc tatgcgacga cgcaggacca
     1021 gctggacttc ctgcggtttc cgctcggcag gctgaccaag gccgaaaccc gggagatcgc
     1081 gcgcggggcc ggcatcgccg tggccgacaa gccggacagc caggatatct gtttcgtgcc
     1141 gcagggccgc tacgacgcca tcgtggaaaa actccggccg gaggcagcgc gccccggcga
     1201 catcgtgcac ctcgacgggc gcgttctggg acgccaccag ggggtgcatc tttacaccat
     1261 tggccagcgg cgcggccttg gcctggcgac gggcgagccc ctgcatgtga tccgcctcga
     1321 tgcggcggcc aaccgcgtga tggtggggcc aaggtccgcg ctctccgcgt cgagactggc
     1381 gctgcgcgat ttcaactggc tcggcgacgg cgtgcccgat gcctgggggc agtccggttt
     1441 cgaggccatg gtgcgcgtgc gttcgactcg aaagccctgt ccggcgctgc tgcattggcg
     1501 cgggggaagg gtcgaggtcg agatatcggg cggcgaggag ggcatcgcgc ccgggcaggc
     1561 ctgcgtggtc tacggccatg aagccagcgg cgcccgcgtc ttcggcggcg gcacgatcga
     1621 tcgtgtcctt tcagcggagg gcgagacatg cgtgcccgcg ccagccggac ctgccggacg
     1681 accggctgag acctcaacaa cggaacacat gtcttgagcg actacgatct tgccacgaat
     1741 gtgcgcgcct acgagcgctg ggctcccttc tacgacaagg tctacacccg gctgctgtcg
     1801 caggcgcgca gcgcctcggt gcgcgcggcc gtggcctgcg gtccggaaat cctggaggtc
     1861 ggtctcggca cggggctttc gctgccggaa tttcccgctg acgtgcgggt gtccggcgtc
     1921 gatctctcgt cgcacatgct ggccaaggcg caggagaaga tcgagcggca gggactgacc
     1981 tgcgtgcgcg gccttgcggt gatggatgcc tgcaatctcg gcttcgcgcc gggccggttc
     2041 gatgcggtct ccgcgcagtt cgtgatcacc ctggtgcccg atccggaaca ggcgctggat
     2101 gaattcgcgc gcgtgctcaa gcctgacggc gagatcatca tcgccaacca catcggcgcc
     2161 gaatccggtc cgatggcggt gttcgagagc ctggccgagc cactggcgaa aaagctcggc
     2221 tggcggccca atttcccgct ccagcgtatc cgggactggg ccggatcgcg gggtttcgag
     2281 gttctgtccg ccgagaaggt caggccgctt ggctatttca tggtgatccg cctcaaacgc
     2341 gcggcgaaga actgaaaaac gcgtgccgcc acccttgact tggaccgggc ggatgcgttt
     2401 atagcgcccc tgcgtccgac cggcggaaac cggatcgggt tgcgtgtggc ggggtagctc
     2461 agctggttag agcagcggaa tcataatccg cgtgtcgggg gttcaagtcc ctctcccgct
     2521 accattcatc ccccggatgg gggaatgcga cgatcgcctt ccccgccccg ctcatcccac
     2581 cgtgacgatg accttgcccc tggcgcggcg ctcggcaagt tcggcgatgg cctcgccggc
     2641 gcgttccagc ggatagcggc tggaaatttc cggccttatc gtgccgtcgc gcagcatgcc
     2701 cagcagttcc atgatgttgg cggcgttgtc gcgcgggcgg cggacggtga aatcgcccca
     2761 gaacacgccg atcacctggc atgccttcag gagggtcagg ttaagcggca gcttgggaat
     2821 tccggcagga aaaccgacga cgaggaaccg gccctcgtag gcgatggcgc gcagggcggc
     2881 ttccgaatag tcgccgccga ccggatcata aatcacgttg gcgccgttcg gtccgaccgc
     2941 atccttgaac acggcagcga gcgccttgcg tccgtcggcg tcgaaggggc cggcgggata
     3001 gatcagcgtg cgatcggcgc cgtgcttctt cgcgacggct gccttttcct cggaagacac
     3061 cgcggcgacc accgtggcac ctgccgcctt gcccagttcc acggccgcga tgccaacgcc
     3121 cccggccgcg ccgagcacga gcagagtttc acccggcttc agcgcggcgc ggtcctttag
     3181 ggcgtgatgc gacgtggcgt aggtcatcag cagcccagcc gcgacatcga agggaatgtc
     3241 ggccgggtag ggaatgcatc gggcctcgtc gagcgcgatc ttttccgcca tgccgcccca
     3301 gccgcagaac ccgatgacgc gatcgcccgg cttcaggcgc ttcacctccg cgcccacggc
     3361 ctcgaccacg ccggcaatct cgccgcccgg cgcgaagggc cgctgcggct tgaactggta
     3421 gagatcacgg atgatcagcg cgtccggaaa attgacgccg acggccttca cggcgatgag
     3481 cgcctgtttc ggccccggcg aaggcgcgtc gatctcgcga agcaccaggg tttcagggcc
     3541 gccgacggtc tcgctcagaa tcgctttcat gcactttccc ctctttgcgg ccaagacaag
     3601 cacgacgcgg cgcgccgggg aagggcatca gcgctcgcgg ttgacgagct ggcgggccag
     3661 ccggttgtgg cgctcgacat ggaccggcag atccagcgtt tccagatggc ctttgcgcac
     3721 cacgacgcgg ccgttgatga tgctccaggc cacatcggcc gaggcacaga acaggagcgc
     3781 ggcgaccgga tcgtgcaacc cgccagcgaa gggcagcttg cgcatgtcga aggcgatcac
     3841 atccgccgac atgccggcgc tgaggtggcc aatgtcgtcg cggccgagca cccgggcacc
     3901 gccgagcgtc gccagttcca gcgcctggcg ggcggacatg gcgtccggcc cgtggccgac
     3961 acgctggagc agcatggcct ggcgcgcctc attgagcatg tggcctccgt cgttggaggc
     4021 cgaaccgtcg acgccgagac cgaccggcac gccctcgcag atcatgcgct tcaccggcgc
     4081 gatgcccgag gcgagccgca tgttggagca gggacaatgg gcgac
//
LOCUS       DAOMXN010000222         4031 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_12104_length_4031_cov_9.989940, whole genome shotgun sequence.
ACCESSION   DAOMXN010000222 DAOMXN010000000
VERSION     DAOMXN010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12104_length_4031_cov_9.989940"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1157)
                     /locus_tag="PK812_12370"
     CDS             complement(<1..1157)
                     /locus_tag="PK812_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRJ70387.1"
                     /translation="MSAKSGHVDGFANANLPPREQWPVLLLDRPEFTYPERLNAAVEF
                     IDRMVEKGFGAQPCLIGRDSAWTYAETLAQVNRIANVLVNDLGFVPGNRVLLRSGNNP
                     MLAACYLAVLKAGGVVVATMPLMRAGELAAVIRKAEISHAFCHAPLIDDLVAAQNDCP
                     MLKRIVAWGDGAPDSLESRMAKASPAFTACDTAQDDTCLIAFTSGTTGVPKGTMHFHR
                     DLLIICDGFGASFLRARPEDRFIGSPPLAFTFGAGGLLLFPLRVGASTVLLEQVGPKD
                     LLAAIARHRATTLFTAPTAYRAMLADLKNHDVSSLRRCVSAGEVLPAPTWEAWFAATG
                     LKITEGLGSTELLHVFIGGEPDTACAGRTGFPVPGYEARVVDEAGVPVPDGT"
     gene            1389..1871
                     /locus_tag="PK812_12375"
     CDS             1389..1871
                     /locus_tag="PK812_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004117125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRJ70388.1"
                     /translation="MDDAEAIVRHEPEGRKAELRLWLRLLTTTNLVSNEIRRRLRAEF
                     NVTLPQFDLLSQLERESGGLRLSDLSKRMMVTPGNLTGLVDRLVAEGQIAREQMPDDR
                     RVVMVRMTDQGRREFSRMARAHQDWIAAMFSGLPPQEQMLLTQELAVLKKSVSDCRAP
                     "
     gene            1980..2300
                     /locus_tag="PK812_12380"
     CDS             1980..2300
                     /locus_tag="PK812_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70389.1"
                     /translation="MIRRSLAALAVLAVFATPALADAIDGDWCAPEGGKSLSIDGPRI
                     RTPGGATIQGQYSRHHFNYTAPAGDPVPGAEVQMILMGEYRVRVVVKGGTDTIWRRCP
                     PGTS"
     gene            complement(2449..2913)
                     /locus_tag="PK812_12385"
     CDS             complement(2449..2913)
                     /locus_tag="PK812_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HRJ70390.1"
                     /translation="MTFWKGRRLTDFTASEWESLCDGCGRCCLVKLEDEDTGRIHATD
                     IACRLFDEGSCRCRDYDNRSSKVPDCVRLTPDVVPTLNWLPPTCAYRLVANGRDLPWW
                     HPLVSGRPETVVEAGVSVLGRTSGLEDDFTLEETMAHVVAWPQRWPRKRNRE"
     gene            2992..>4031
                     /locus_tag="PK812_12390"
     CDS             2992..>4031
                     /locus_tag="PK812_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRJ70391.1"
                     /translation="MREFRTITWTTRLRRRWLDANAMLDSSVFGSARNAASAYASYSA
                     LMDRFKVAGARAWVTFLLSEGLTWGTLGLVVLLMLGQPAFREVDDDNWLKRQELAVTF
                     FDRHGTEIGKRGIKNDDSVTLEDLPDQLIKAVLATEDRRFYEHFGIDIPGLVRALLAN
                     ARANSVVQGGSTLTQQLAKNLFLTNERSLERKIKEAFLALWLEARLTKREILKLYLDR
                     AYMGNGTFGVQAAATQYFGKNIKDISLAEAAMLAGLFKAPSKFAPHVNLPAARARADA
                     SFCSTSRSARSVSSRRPSAVSSAISASASAACRVSSSASNRAICSRASPIRCSSRAMS
                     RDSCSTRWRNSRS"
BASE COUNT          676 a         1341 c         1359 g          655 t
ORIGIN      
        1 gtgccgtcgg gcacgggaac gccggcctcg tccaccacgc gggcctcata gcccggcacg
       61 gggaaaccgg tgcgtccggc gcaggccgtg tccggctcgc cgccgatgaa cacatgcagg
      121 agttcggtgg agccgagccc ctcggtgatc ttcaggccgg tcgcggcgaa ccaggcctcc
      181 caggtcggcg cgggcagcac ttcgccggcg gaaacgcagc ggcgcaggct ggacacgtcg
      241 tggttcttca ggtccgccag catggcgcgg taggccgtgg gggcggtgaa cagcgtcgtc
      301 gcgcggtgcc tggcgatggc ggcgagcagg tccttcgggc cgacctgttc cagcagcacg
      361 gtggaggcgc cgacccgcag cggaaacagc agcagcccgc cggcaccgaa ggtgaaggcc
      421 agcggcggcg agccgatgaa ccggtcctcg ggccgggcgc gcaggaacga tgcgccaaag
      481 ccatcgcaga tgatcaggag gtcgcggtgg aaatgcatcg tgcctttcgg cacgccggtg
      541 gtgccggagg tgaaggcgat gaggcaggtg tcgtcctgtg cggtatcgca ggcggtgaac
      601 gccgggctgg ccttggccat gcgcgattcg aggctgtccg gcgcgccatc gccccaggcg
      661 acgatgcgct tcagcatcgg gcagtcgttc tgcgccgcca ccagatcatc gatcagcggg
      721 gcatggcaga aggcatgcga aatctcggcc ttgcggatca ccgccgccag ttcgccggca
      781 cgcatcagcg gcattgtggc aaccacgacg ccgccggcct tcagcaccgc gagatagcag
      841 gcggcgagca tcggattgtt gccggagcgc agcagcacgc ggttgccggg cacgaagccg
      901 agatcgttga ccagaacgtt ggcaatgcgg ttcacctgcg ccagcgtctc ggcataggtc
      961 caggcgctgt cgcggccgat caggcagggc tgagcgccaa agcccttctc gaccatgcgg
     1021 tcgatgaact cgaccgccgc gttcagccgt tcgggatagg tgaattccgg acggtccagc
     1081 agcagcaccg gccactgctc gcgcggcggc aggttggcat tggcgaagcc atcgacatga
     1141 cccgatttcg cagacatcga ccctccccaa gacccgcgcc cgcaggcgcg cagaataccg
     1201 gcgcccggcc tcagggcaag caccgccttt tcttttatga ttataaatta taagcttaaa
     1261 gctttctgtc aatcgcggca gtgcttgaaa tttcaacatt tcaggttcaa agtttcacgc
     1321 agagggccgg cacggcgcgc tcaagcgcga gacggcgaac gggcgcaatc gcggggagcg
     1381 aagcgggaat ggacgacgcc gaagccatcg tgcgtcacga accggagggc cggaaggccg
     1441 agcttcggct ctggctgcgg ctgctgacga cgacgaatct cgtctccaac gagatccgcc
     1501 ggcggctgcg cgccgagttc aacgtcacgc tgccgcagtt cgaccttctg tcccagcttg
     1561 agcgggaatc gggcgggctg cgcctcagcg acctgtccaa gcgcatgatg gtgacgcccg
     1621 gcaatctcac cggactggtg gaccggctgg tcgcggaagg gcagatcgcg cgcgagcaga
     1681 tgccggacga ccggcgggtg gtcatggtgc gcatgaccga tcagggcagg cgcgagttct
     1741 cgcgcatggc ccgggcgcat caggattgga tcgccgcaat gttctccggc ctgccgccgc
     1801 aggaacagat gctgctgacg caggagctgg cggtactcaa gaaatccgtc agcgactgcc
     1861 gcgcgccctg acctcgggac cgcgcctttg gcgcgatcac gcctttgtgg ccggcccgga
     1921 cttgcgcatg cgggggctcc gggtccaggt tctggcatcg atgctccgag aggccctcaa
     1981 tgatccgccg cagcctcgcc gcccttgccg tcctcgccgt tttcgcgacg cccgctctcg
     2041 ccgacgccat cgacggcgac tggtgcgcgc cggaaggggg caaaagcctg tccatcgacg
     2101 ggccgcgcat ccgcacgccc ggcggcgcga ccatccaggg ccagtattcc cgccaccact
     2161 tcaactacac cgcgccggcc ggcgatccgg tgcccggcgc ggaagtgcag atgatcctga
     2221 tgggcgagta ccgcgtgcgc gtcgtcgtga agggcggaac cgatacgatc tggcgccgct
     2281 gcccgcccgg aacgagttga ggcaatcggc gctcggtggc gtcagtccgg ggcgcttcgc
     2341 ggaagcgaac ccggactcga tgtcttgcgg tcgcccagaa agagggattc cggccctggc
     2401 ttcgccgacc cggattgacg ccggcgcatc aactcccgac tgccgcccct actcccggtt
     2461 ccgcttcctc ggccagcgct gcggccaggc gacgacatgc gccatggttt cctccagcgt
     2521 gaagtcgtct tccaggcccg aggtgcgccc gaggacggag acgccggcct cgaccacggt
     2581 ctcgggccgg cccgagacca ggggatgcca ccacggcagg tcgcgcccgt tggcgacgag
     2641 gcgataggcg caggtcggcg gcagccagtt cagcgtcggc acgacatccg gcgtcaggcg
     2701 cacgcaatcc ggcaccttgc ttgagcgatt gtcgtagtcg cggcagcggc aggacccctc
     2761 gtcgaacagg cggcaggcaa tgtcggtggc gtgaatccgg ccggtgtcct cgtcttccag
     2821 cttgacgagg cagcagcgtc cgcagccgtc gcagaggctt tcccattcgc tggcggtgaa
     2881 atcagtaagg cgacgcccct tccagaatgt catgcgtccc cggcatcgcg cttgcagttg
     2941 gtcacggaaa aggccatctc tctttgttaa gcgttcctta acggcacagg catgcgcgaa
     3001 ttcaggacga tcacctggac gacacggctc cggcggcgct ggctcgacgc caacgccatg
     3061 ctggattcga gcgtcttcgg ctcggcgcgc aacgccgcgt ccgcctacgc aagctattcc
     3121 gccttgatgg accggttcaa ggtggccggt gcccgcgcct gggtgacgtt cctgctcagc
     3181 gagggtctga cctggggcac gctcggcctg gtggtgctgc tgatgctcgg ccagccggcc
     3241 ttccgcgagg tggacgacga caactggctg aagcgacagg aactggccgt caccttcttt
     3301 gaccggcacg ggaccgagat cggcaagcgg ggaatcaaga atgatgattc cgttacgctc
     3361 gaggatctgc ctgatcagct gatcaaggcg gttctggcga ccgaggatcg gcgtttctac
     3421 gagcatttcg gcatcgatat ccccggtctg gtgcgcgccc tgctggccaa tgcccgcgcc
     3481 aattccgtcg tgcagggcgg ctcgaccctg acgcagcagc tcgccaagaa cctgttcctg
     3541 accaacgagc gttcgctcga acgcaagatc aaggaagcct tcctcgcgct ctggctggag
     3601 gcgcggctga ccaagcggga gatcctgaaa ctctatctgg accgggccta catgggcaac
     3661 ggaaccttcg gcgtccaggc tgccgcgacg cagtatttcg gtaaaaatat caaagatata
     3721 tccttggccg aggcagcgat gctggcaggg ttgttcaagg cgccgtcgaa gttcgcgccg
     3781 catgtcaacc tgccggcggc gcgcgcccgg gccgatgcca gcttctgctc gacctcccgc
     3841 agcgcccgct cggtctcctc gcggcgtccc tcggcggtct cctccgcgat atcggcctcc
     3901 gcctcggcgg catgcagggt ctcgagctcg gcctcgaacc gcgcgatctg ctcgcgcgcc
     3961 tcgccgatca gatgctcctc gcgggcgatg tcgcgcgaca gttgctcgac acggtggcgc
     4021 aactcgcgct c
//
LOCUS       DAOMXN010000223         4024 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_12135_length_4024_cov_9.853867, whole genome shotgun sequence.
ACCESSION   DAOMXN010000223 DAOMXN010000000
VERSION     DAOMXN010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12135_length_4024_cov_9.853867"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="PK812_12395"
     CDS             <1..583
                     /locus_tag="PK812_12395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRJ70392.1"
                     /translation="NAAYAMGDVRFGPLRLLAVGSIIPRKAYDVMISGLTLLTDLRWR
                     LDIVGATQHAPEAVAALRRQITGAGLEDRVRLCGPLGAEALADAYTGADLFVMTSLYE
                     GYGMVIAEAMARGLPIVAAAGGAIADTLPDEAGIKVPPGDAAAFASGLRRLIVDSDLR
                     QRYAERSWQAGQSLPGWADTAGIIAQVIRRVST"
     gene            580..1476
                     /locus_tag="PK812_12400"
     CDS             580..1476
                     /locus_tag="PK812_12400"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRJ70393.1"
                     /translation="MSFSADWLALREPADHRSRNGDLAAAVQAHFAGRGEVRVTDFGC
                     GTGSNLRATCALLPAVQHWTLVDYDPALLAEARRVLARWAESANEPDGRLILTHRGKR
                     IEVAFQQVDLNSDLDAALEPTPDLVTASALFDLISETWIRSFARKLAARRCAFYTVLT
                     YDGRDEFAPPHAADHAVIAAFAAHQGGDKGFGPAAGPRGADVLASAFAEAGLAVREGD
                     SPWRLGSGDAALARELLDGVANAVRETGRLSPAEIADWLAFRQAHLGDRDSLMLTGHR
                     DTFAVPRSSIVPGASASARRRG"
     gene            complement(1433..1969)
                     /locus_tag="PK812_12405"
     CDS             complement(1433..1969)
                     /locus_tag="PK812_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HRJ70394.1"
                     /translation="MNEPIEPYSPLARRLHWLTALAIVVAFPLGVALDKASPAMMDTL
                     YRAHWSFGLLALFLAVPRVANQLRGDTPGRYPGLTRFEAVASSIVHKLLYLLILAVPL
                     AGWLGKSAYGGAITVFGLFDMPALLSQNQTLGERILTVHTSLAKLLALCIVLHVAGAL
                     KHAFINRDGVLKRMLPGR"
     gene            2215..3333
                     /gene="ribA"
                     /locus_tag="PK812_12410"
     CDS             2215..3333
                     /gene="ribA"
                     /locus_tag="PK812_12410"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II RibA"
                     /protein_id="HRJ70395.1"
                     /translation="MGKVANILGLFGDVGQTNVERAISEFRSGRPVVLRKNDDALIAI
                     GVEAFDAGVAAAVEAFAKGRGRLVLPAARLRRLGREAQEPGSLAFPVVDLERIETLSL
                     KLDARVDAPVKRVTDLETMALELAALSLTLPGVFVVDVEGAAPAGLVSVSADDVRGYR
                     SAATANLQLVSRSPVPLEYASKAEFVVFRGGEGLRDQVAIIVGEPDLKEPVAVRLHSA
                     CLTGDLFGSLKCDCGDQLRHTVKNMAEGEGGILLYLDQEGRGNGIANKMRAYKLQSQG
                     WDTYDADEALGFDLDQRHFDFAADMLKQLGVTSVKVMTNNPIKIGALKGAGLDVASDA
                     RVLGRATAENVRYLASKRDRAGHFIDFETMTALARQQD"
     gene            3386..>4024
                     /locus_tag="PK812_12415"
     CDS             3386..>4024
                     /locus_tag="PK812_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70396.1"
                     /translation="MTQPPSSRWPLWPLLGALCAAWLVFCWPWLTGRVTVPWDAKAHF
                     YPQLVFLAQSLAAGERPFWTPFVFSGAPQIADPQSLMFSPPHFLLARFNAAPGFAAAD
                     AVPFAALLFGAFAIVLYFRDRNWHPAGAMVAALAFMFGGSAAWRIQHVGQVMSLAYFP
                     MALWLLDRGLARSSLFYGAAAGIVAGFMALGRDQVAGLATLVLGAYVLTHLLD"
BASE COUNT          606 a         1292 c         1381 g          745 t
ORIGIN      
        1 caacgccgcc tacgccatgg gcgacgtgcg cttcgggccg ctgcggctgc tggcggtcgg
       61 atcgatcatc ccgcgcaagg cctatgacgt gatgatctcc ggtctgaccc tgctcacgga
      121 ccttcggtgg cggctcgaca tcgtcggagc gacgcagcat gcacccgagg cggtggccgc
      181 gctgcgccgg caaatcacgg gagccggtct cgaagatcgc gtgcgcctgt gcggcccgct
      241 cggcgccgaa gcgctcgcgg atgcctatac cggcgcggac cttttcgtga tgacctcgct
      301 ctacgaaggc tacggcatgg tgatcgccga ggccatggcg cgcggcctgc ccatcgtcgc
      361 ggcggcaggc ggcgcgattg ccgacacgct tcccgacgag gccggcatca aggttccgcc
      421 gggcgacgcg gcggcgttcg catcgggcct gcgccggctg atcgtggaca gcgacctcag
      481 gcaacgctac gccgaacgct cctggcaggc cggtcagtcc ttgccgggtt gggcggatac
      541 ggccgggatc atcgcacaag tcatcaggcg ggtttcgaca tgagtttctc ggcggactgg
      601 ctggcgttgc gcgagcctgc ggaccatcgc agccgcaatg gtgatctggc agcggcggtg
      661 caggcacatt tcgccggccg gggtgaggta cgcgtcaccg atttcggctg cggcaccgga
      721 tcgaacctgc gggcgacctg cgctctgctg cccgccgtgc agcactggac gctggtcgac
      781 tacgatcccg ctctgctggc tgaggcgcgc agggtgctgg cgcgctgggc cgaaagcgcg
      841 aacgaaccgg acggccggct gatcctgacg catcggggaa agcgcatcga ggttgcgttc
      901 cagcaggtgg acctgaactc ggacctcgat gccgcactcg agccgacgcc cgaccttgtg
      961 accgcttccg cccttttcga tctgatttcc gagacctgga tcaggagttt cgctcggaaa
     1021 ctcgcggcaa ggcgctgcgc gttctacacc gtgctgacct atgacgggcg cgacgaattt
     1081 gccccgcccc atgccgccga tcatgccgtg atcgccgcgt tcgctgccca tcagggcgga
     1141 gacaagggct tcgggcctgc ggccgggccg cgcggcgcgg atgtcctggc cagcgccttc
     1201 gcggaggccg gtctcgctgt gcgggaggga gacagcccct ggcgcctcgg gtcgggcgat
     1261 gccgctctgg cgcgggaact cctggacggc gtcgccaacg ctgtgcgcga aaccggtcgc
     1321 ctgtcaccgg ccgagatcgc ggactggctc gccttccgac aggcacatct tggggatcgc
     1381 gacagcctca tgctgacggg tcaccgcgat acgttcgcgg tgccgcgatc gtctatcgtc
     1441 ccgggagcat ccgcttcagc acgccgtcgc ggttgatgaa tgcgtgcttg agtgcgccgg
     1501 cgacatgcag aacgatgcac agggccagca gcttcgccag cgacgtgtgg acggtgagga
     1561 tgcgttcacc gagcgtctga ttttgggata gcagcgccgg catgtcgaac aatccgaaga
     1621 cggtgatggc tccaccatag gcagacttgc caagccagcc ggcgagcggc acggcgagga
     1681 tcagcaggta gagcagcttg tgcacgatcg acgacgccac cgcttcgaac cgcgtcaggc
     1741 ccggatagcg tcccggcgtg tcgccccgca gttggttggc gacacgggga accgccagga
     1801 acaaggccag aaggccgaac gaccagtgtg cgcgatacag cgtgtccatc atggcgggcg
     1861 aggccttgtc caacgccacg ccgagcggaa aggcgacaac gatcgccagc gcggtcagcc
     1921 aatgcaggcg ccgcgccaga ggtgaataag gctcaatcgg ctcgttcatg cccgcgtcct
     1981 gtgatgcctg ttcattattg acgcttggag aaagcggtgc aagtcgctcc ccttatgcgc
     2041 attgtagcaa atccattttg cagaattaca cttatccgac ccccgcggat gccgccgtta
     2101 agtgaatcga gcattgccag acatctgccg ttcattttcg ttcaggttta tggcatggca
     2161 tgcgcccggt agtgcacgct tgggacagcg gcaagtggcg tagttgaggg agcgatgggt
     2221 aaggttgcga atattctggg tctgttcggc gatgtcgggc aaaccaatgt ggagcgggcc
     2281 atctccgagt tccggtcggg tcggcccgtg gtgctgcgca agaatgacga tgccctgatc
     2341 gcgattggcg tcgaggcatt cgatgccggc gtcgccgcgg ccgttgaggc gttcgccaag
     2401 gggcgtgggc ggctcgtgct gccggcggcg cggttgcgtc gccttggccg cgaggcgcag
     2461 gagcccggct ctctggcatt cccggtggtt gatctcgaac ggatcgagac cttgtccctc
     2521 aagctcgacg cgcgggtgga tgcgccggtg aaacgcgtca ccgatctcga gaccatggcg
     2581 ctcgagctgg cggccctgtc gctcaccctt cctggcgtct tcgtggtgga tgtcgagggg
     2641 gcagcgccgg cgggtcttgt gtcggtttcg gccgatgacg tccgcggcta ccgttccgcc
     2701 gccacggcga acctacaact ggtcagccgc tcgcccgtgc cgctagagta cgcctccaag
     2761 gccgagttcg tggtgttccg cggcggcgag ggcctgcgcg atcaggtagc gatcatcgtt
     2821 ggcgagccgg acctgaagga acctgtggcg gttcggctgc attcggcctg cttgacgggc
     2881 gacctgttcg gcagcctgaa gtgcgattgc ggcgaccagc tgcgccacac cgtcaagaac
     2941 atggcggaag gcgagggcgg tatcctgctc tacctggacc aggaagggcg cggcaacggc
     3001 atcgcgaaca agatgcgggc ctacaagctg cagtcgcaag gctgggacac gtatgacgcc
     3061 gacgaggcgc tcggcttcga tctcgatcag cggcacttcg atttcgccgc cgacatgctg
     3121 aagcagctcg gcgtgacttc ggtgaaggtc atgaccaaca acccgatcaa gatcggcgcg
     3181 ctgaagggtg ccggccttga tgtggcttcg gatgcacgcg tgctcggccg cgccacggcg
     3241 gagaacgtgc gctaccttgc ctccaagcgg gatcgcgccg gtcatttcat cgatttcgag
     3301 accatgaccg cgctggcgcg ccagcaggat tgacgcgata aggtccggct gtcgattgtt
     3361 cccgcgacag ccggatttcg attccttgac ccagcctccc tcatcccgct ggccgctctg
     3421 gcccctgctt ggcgcgcttt gcgccgcctg gctcgtgttc tgctggccct ggcttaccgg
     3481 ccgcgtcacc gtaccctggg acgccaaggc gcatttctac ccgcagttgg tgtttctggc
     3541 gcagtcgctt gctgccggtg aacggccgtt ctggacgccg ttcgtcttct ccggcgcgcc
     3601 gcagatcgcc gatccgcagt cgctgatgtt ttccccgccg catttcctgc tggcgcggtt
     3661 caatgccgca ccgggtttcg cggcggcgga cgcggtgccg ttcgccgcgc tgctgttcgg
     3721 cgccttcgcc attgtcctct acttccgtga ccggaactgg cacccggcgg gcgcgatggt
     3781 ggcggcgctc gccttcatgt tcggcggttc ggcggcctgg cgcatccagc atgtcgggca
     3841 ggtcatgagc ctggcctatt tcccgatggc cctgtggctg cttgatcggg ggctggcgcg
     3901 gtcttccttg ttctacggcg ccgccgccgg aatcgtggcg ggattcatgg cgctgggccg
     3961 cgatcaggtc gcggggctcg cgacgctggt gctcggcgcc tacgtgctga cgcatctgct
     4021 cgac
//
LOCUS       DAOMXN010000224         4024 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_12136_length_4024_cov_9.333837, whole genome shotgun sequence.
ACCESSION   DAOMXN010000224 DAOMXN010000000
VERSION     DAOMXN010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12136_length_4024_cov_9.333837"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="PK812_12420"
     CDS             <1..613
                     /locus_tag="PK812_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70397.1"
                     /translation="PPQDLPLAADGGDGGALPPASRQRALPVVILAMASSGFVSWGLE
                     LHAMEILLAFGVTNAVALMIVGLRGPISLISRSADIVLGGRFAALDIAIFGCFAMFLS
                     LLAAAVGSGVAGAITFMILFASGTGIMTIARATMQLALFGPQGYGTLAGRIGRPAHFV
                     YAIAPPIFGFVSEYVGLGASLALAMVAMTIAIGALMALKRMTV"
     gene            complement(635..883)
                     /locus_tag="PK812_12425"
     CDS             complement(635..883)
                     /locus_tag="PK812_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70398.1"
                     /translation="MDVNIVYECIGYGTGRAVTSQKFAMSPSRIPARFILETAKDPVA
                     YCIEAYAERQPDRAGRIDRMGRALILRHLEREAGLRSH"
     gene            complement(1102..1497)
                     /locus_tag="PK812_12430"
     CDS             complement(1102..1497)
                     /locus_tag="PK812_12430"
                     /inference="COORDINATES: protein motif:HMM:NF040427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6455 family protein"
                     /protein_id="HRJ70399.1"
                     /translation="MTTIHTASRSPLEGLMHWFREHLRGNELSHLDRSELELMARDLN
                     ISSDDLTRLSTEEPKDISLMKQMMALHGLDYDALRKEYSGVVRDMEVTCSRCADRKLC
                     EHDLATGADAHTCDTYCPNADTMKAFAGG"
     gene            1655..2353
                     /locus_tag="PK812_12435"
     CDS             1655..2353
                     /locus_tag="PK812_12435"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HRJ70400.1"
                     /translation="MSVVNSVRKMLVPVHPEGYVFIAAAALAAIVGGWIWSPLGWLFW
                     IAAGFIAYFFRDPARVTPVRDSLVIAPADGRVDMVGFFTPPAELGLGDKPLPRITIFL
                     SVFDVHIQRAPIGGKVTKIAYTPGLFLSADLDKASEDNERNGVVIENAHGRFGVVQIA
                     GLIARRIVCFVKEGEELAPGERYGLIRFGSRVDLYLPDNARPMVGVGQRAIGGETVLA
                     DLKAQEPQRTFRVG"
     gene            2385..3185
                     /gene="pssA"
                     /locus_tag="PK812_12440"
     CDS             2385..3185
                     /gene="pssA"
                     /locus_tag="PK812_12440"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HRJ70401.1"
                     /translation="MSQDTPLPPADKPRLRNLRGVPVRVLIPNMVTLLVLAAGLTAIR
                     FAVEGRLEHSVLAIFAAAILDGLDGRIARLLKGASRFGAELDSLADFISFGCAPALVL
                     YFWTMHDLSTFGWLAALALAFAAALRLARFNVMSEDLTRPEWTKQFFTGVPAPAGAML
                     ALLPINLYLAGLPKFSGHALVVSIYTVVVAALMVSRLPTLSGKGKAGPVPSEKVIPIV
                     AAVILVVVLLVSHPFHTMAGLVLVYFALIPVGLARHARLAAAEKSVTP"
     gene            3182..>4024
                     /locus_tag="PK812_12445"
     CDS             3182..>4024
                     /locus_tag="PK812_12445"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HRJ70402.1"
                     /translation="MSDAARIAVLGSVNVDLVVRAGRLPRPGETLHGESFTLALGGKG
                     ANQACAAARLGADVCFIARTGTDGFGDFACDRLAAFGLGLDHLARESAVGAGIAVIAV
                     DSRGENAITIVSGANARLSPADAEAARAELSGSAVLLVQCETPFDASLAAARIVASAG
                     GRVILDPAPAPQAGIPVDLMALADLVTPNETEAALLTGIPVRDRDSALAAARALRESG
                     ARAAVVKLGGAGLVYADADSEGFLPPFAVRVVDTVAAGDCFNAGLAVALAGGANLLAA
                     LSFAT"
BASE COUNT          523 a         1352 c         1363 g          786 t
ORIGIN      
        1 cccgccacag gatctcccgc tcgccgccga cggtggagac ggcggggccc tgccaccggc
       61 ttcgcgccag cgcgcgctgc ccgtcgtgat cctggcgatg gcctccagcg gctttgtcag
      121 ctggggcctt gaactgcatg ccatggaaat cctgctggct ttcggcgtga ccaatgcggt
      181 ggcgctgatg atcgtgggcc tgcgcgggcc tatctccttg atctcccgtt cggccgacat
      241 cgtgctgggg gggcggttcg ccgccctcga catcgcgatc tttggctgtt tcgcgatgtt
      301 cctgtcgctg ctcgccgccg ccgtcgggtc cggtgtggcg ggcgcgatca ccttcatgat
      361 tctgttcgca tccgggaccg gcatcatgac catcgcacgc gccaccatgc agctcgcttt
      421 gttcggtccg cagggctacg gcacgctcgc cggacgcatc ggacgaccgg cacatttcgt
      481 ctatgccatc gcgccgccga tcttcggatt tgtttcggaa tatgtcgggc ttggcgcctc
      541 gctggcgctt gccatggtgg cgatgaccat cgccatcggc gcgttgatgg cgctcaagcg
      601 gatgaccgtg tgatcgcccc ggccggctgc cccgtcagtg gctgcgcagc ccggcttcgc
      661 gctccaggtg cctgagaatc agcgcccggc ccatgcggtc gatccgtccg gcgcggtccg
      721 gctgccgctc ggcataggcc tcgatgcagt aggcgacggg gtcctttgcc gtttcgagaa
      781 tgaagcgggc aggaatgcgg gacggagaca tcgcgaactt ctggctggtg acagcgcgcc
      841 ccgtgccata gccgatgcat tcgtagacga tgttgacgtc catgacggcc tcccgactgt
      901 cgcgttcgat tcgatgcacc tcctgatggc gtcgaattgt gtcaacaata ctacgacgaa
      961 agtctaggaa caagttcgtg accacgcgtc acgacacccc ctgcgctcaa gactggcggg
     1021 gaagaggcac gccatgcggc tggcgcgtgc gatgtgccgg caagcgccgg caacgcctcc
     1081 ctgccggcgc cgccgtatgc ctcagcctcc ggcgaaggcc ttcatcgtgt cggcgttggg
     1141 gcagtaggtg tcgcaggtgt gcgcatcggc gccggtggcg aggtcgtgct cgcacagctt
     1201 gcggtcggcg cagcgcgagc aggtaacctc catgtcgcgc acgacgccgg aatactcctt
     1261 gcgcagagcg tcatagtcga ggccgtgcag cgccatcatc tgcttcatca gcgagatgtc
     1321 cttcggctct tcggtggaaa gccgggtcag gtcatcgctg gaaatgttca ggtcgcgcgc
     1381 catcaactcc agttcgctgc ggtcgagatg gctgagttcg ttgccgcgca ggtgctcccg
     1441 gaaccagtgc atcaggcctt cgagcggcga acggctggcg gtgtgtatgg tcgtcatggg
     1501 catagttccc ccttcactct cgccacagaa tgccgcggac gccggcgctc cgcactgatc
     1561 cggatcaaat tcgttgccgg ccggtccctg aggccgcgtc gcgcttggct tttgcgctgt
     1621 gtttcgatag ggggtgcggc acaggaggat cgcgatgtcc gtcgtcaatt ccgtgcgcaa
     1681 gatgctcgtg cccgtgcatc ccgaaggcta tgtcttcatt gcggcggcag ccctggccgc
     1741 catcgtcggc ggctggatct ggtcacccct gggctggctg ttctggattg ccgcggggtt
     1801 catcgcctat ttcttccgcg atcctgcgcg cgtgacgcct gtgcgcgaca gcctggtcat
     1861 cgcgcccgcc gatggtcgtg tcgacatggt cggcttcttt accccgccgg cggaactggg
     1921 ccttggcgac aagcctttgc cgcgcatcac catcttcctc tcggtgttcg acgtgcacat
     1981 ccagcgtgcg ccgatcggcg gcaaggtgac gaagatcgcc tacacgcccg gcctgttcct
     2041 ctcggccgat ctcgacaagg ccagcgagga caacgagcgc aacggcgtcg tcatcgagaa
     2101 tgcccatggc cggttcggcg tggtgcagat cgccggcctc atcgcccggc ggatcgtctg
     2161 tttcgtgaag gaaggcgagg aactggcccc cggcgagcgc tacggactga tccggttcgg
     2221 ctcccgcgtc gacctttacc ttccggacaa cgcccggccg atggtcggcg tcggccagcg
     2281 cgccatcggt ggcgagaccg tgcttgccga cctcaaggcg caggagccgc agcgcacctt
     2341 ccgggtgggc tgacggcggg cggcgctcgt cgtatggtga cggcatgagc caggacaccc
     2401 ccctcccgcc agccgacaag ccccgccttc gcaatctgcg cggcgtgccc gtgcgcgtgc
     2461 tgattcccaa catggtgacg ctgttggttt tggctgcggg actgacggcg atccggttcg
     2521 cggtggaggg ccggctcgaa cactcggtgc tggcgatctt cgcggccgcc atcctcgacg
     2581 ggctggacgg gcgcatcgcg cgtctgctca agggcgcctc gcgtttcggc gcggaactcg
     2641 attcgctggc ggacttcatc agcttcggct gcgcgccggc cctggtcctg tatttctgga
     2701 ccatgcatga tctgtcgacc ttcggctggc tcgcggcgct ggcgctggcc ttcgctgcgg
     2761 cgctcaggct ggcgcgcttc aacgtgatgt cggaggatct gacgcggccg gaatggacca
     2821 agcagttctt caccggcgtt cccgcgccgg ccggcgcgat gctggcgctg ctgccgatca
     2881 acctctatct cgccggcctg ccgaagttct ccggccatgc cctggtcgtt tccatatata
     2941 cggtcgtcgt ggcggcgctg atggtgtcgc gcctgccgac cctgtcgggc aagggcaagg
     3001 ccggtccggt cccgagcgag aaggtcattc ccatcgtcgc cgccgtgatt ctggtggtgg
     3061 tgctgctggt cagccatccc ttccacacca tggccgggct ggtgctggtc tatttcgcgc
     3121 tgattccggt cgggctcgcc cgccatgccc ggctcgccgc cgccgagaag tccgtcacac
     3181 catgagcgac gcggcgcgga tcgccgttct ggggtccgtg aacgtcgatc tggtggtgcg
     3241 cgccggacgt ctgccacgcc cgggcgagac cctgcacggc gagagtttca ccctcgcgct
     3301 cggcggcaag ggggcgaatc aggcctgcgc cgcggcccgg ctgggggcgg atgtctgctt
     3361 catcgcccgc acgggaaccg acgggttcgg cgatttcgcc tgtgatcggc tggcggcgtt
     3421 cggcctcggc cttgatcatc tcgcgcgcga gtcggcggtc ggggcgggta tcgcagtcat
     3481 cgctgtcgat tcccggggcg agaacgccat caccatcgtt tccggcgcga acgcccgcct
     3541 gtcgcccgcc gatgccgagg cggcgcgggc ggagctgagc gggtccgcgg tgctgcttgt
     3601 gcaatgcgaa accccgttcg acgcctccct ggcggcggcg cggatcgtcg cgtctgccgg
     3661 tggccgcgtg attctcgatc cggcaccggc gccccaggcc ggcattccgg tcgatctcat
     3721 ggcgctggcc gacctggtta ccccgaacga gacggaagcg gcgcttctca ccggcattcc
     3781 cgttcgcgat cgcgattcgg ctctcgccgc cgcccgcgcg ctgcgggaat cgggggctag
     3841 ggcggctgtg gtgaagctcg gcggcgcggg tctggtctat gccgatgccg attcggaagg
     3901 gtttctgccg ccgttcgcgg tccgtgtggt ggataccgtc gccgccggcg actgtttcaa
     3961 cgcgggcctt gccgtcgcgc tggccggcgg cgcgaatctg ctcgccgccc tcagcttcgc
     4021 cacc
//
LOCUS       DAOMXN010000225         3922 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_12572_length_3922_cov_12.595035, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000225 DAOMXN010000000
VERSION     DAOMXN010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3922
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12572_length_3922_cov_12.595035"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="PK812_12450"
     CDS             complement(<1..408)
                     /locus_tag="PK812_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRJ70403.1"
                     /translation="MRRHSRSGPLGLFSSRAEKDGYHHGRLKEALLDAAEALIAARGP
                     AGLTLSEMARMSGVTSAAIYRHYADLQSLIGAVARRGFEEFGARLKGAMAAGGPGPDG
                     LRGMGRAYLAFARERPGAYAAMFSSTIAYSDPDL"
     gene            530..1912
                     /locus_tag="PK812_12455"
     CDS             530..1912
                     /locus_tag="PK812_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRJ70404.1"
                     /translation="MHASAPGVSQSESDTNLTSRRAAWQARALDGATRALLAEDAQYF
                     LRQSVSTPCLNAIAKAEGVFIEDLAGRRYMDFHGNNVHHIGYGHPRLKKAIAEEMDRL
                     PFAPRRYASATSVALARKLAEIAPGRLSRMLFTTGGSDAIEVAVKIARTATGRHKTLS
                     FWDAFHGAGFGASALSGEALFRSGRAAPLIAGDQHVAPFACYRCAYGHDCDASGRPRL
                     EVCKLSCARMVDYVLSRETDVACVVAEPARAVPYVPPPGFWQAVREACDRHGTLLVFD
                     EIPTGLGKTGRMFACDHDGVEPDILVMGKALGGGILPIAAVITRPDLDVGGDYAFGHY
                     THEKNPVTARAALTTIEIIEDEGLVENARRVGDAALERLDAMKRRLPAIGDVRGRGLL
                     LGIDLVADRDSKAPAGDLAEAVLYRALDAGLSFKTTMGNVLTLTPPLTVSTDQMMAAL
                     DIIETAITAG"
     gene            complement(2287..3543)
                     /locus_tag="PK812_12460"
     CDS             complement(2287..3543)
                     /locus_tag="PK812_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HRJ70405.1"
                     /translation="MLRALDLAEALDAGDLAVSGVVELIAEAIAAREADIGAFAHLDL
                     DALRAAALRPQSGPLHGMPVAFKDIIDTAGLPTEYGSPVWRGWQPKADATIVSMTQAA
                     GGLVLGKAVTTELAFLHPSPTRNPHDPARTPGGSSSGSAAGVAAGMMPLAFGTQTGGS
                     VIRPASYCGVAAIKPSFRLLPMVGIKAGSWTMDTLGLFGARVRDVAFGLGAVSGRDLR
                     VDGRDFGTPTFGVLDMTFAGEPEPESQAALRFAIRAAENAGANVVSIAAPPTFAAAQD
                     AHATIYQFEGALAARWELDHHADALSEEFRSFMNPVRPASVAEYDAARGAAKAARRQS
                     KALFEDVDVLLTCAAPGVAPDRSTTGVATFNKLFTLLGTPALNVPGYETADGLPVGVQ
                     VVAGFGEDLMALAAADFLEQALAQAE"
     gene            3856..>3922
                     /locus_tag="PK812_12465"
     CDS             3856..>3922
                     /locus_tag="PK812_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013499886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HRJ70406.1"
                     /translation="MPKLKTKSGAKKRFKITATGKV"
BASE COUNT          672 a         1297 c         1323 g          630 t
ORIGIN      
        1 caggtccgga tcggaatagg cgatggtgga ggagaacatg gcagcgtagg ctcctggccg
       61 ctcgcgcgca aaggcgaggt aagcccgccc catgccccgc aagccgtcgg ggcccggtcc
      121 accggcagcc atcgcgccct tgagccgtgc gccgaactct tcaaatcccc gccgtgccac
      181 agcgccgatc agggactgaa ggtctgcata gtggcgatag atcgcggccg aagtcacccc
      241 ggacatgcgg gccatctctg aaagcgtcag cccggcgggt ccccgtgcgg cgatcagggc
      301 ctccgcagcg tcgagcagcg cttccttcag gcggccgtgg tgatagccgt ccttttcggc
      361 gcgggaactg aagagcccga ggggaccgga acgggaatgg cggcgcatgg catcggttta
      421 gcggcaaatc gcttcggagc aaaggacggc gagagaagcc gcgttgcgga ggactggctc
      481 cgctgtcata cggatgaggg ttacagccac cgccatcgca gagcccgcca tgcacgcatc
      541 cgcccccggc gtcagccaat cagagagcga caccaacctg accagccgca gggcggcctg
      601 gcaggcgagg gccctggacg gcgcaacccg cgcgcttctc gccgaggacg cgcaatactt
      661 cctgcgacag tccgtctcga cgccctgcct gaatgccatc gccaaggcgg aaggcgtctt
      721 catcgaggac ctggcaggcc gtcgctacat ggatttccac ggcaacaacg tgcatcacat
      781 cggttacggt catccgcgtc tgaaaaaggc gattgcggaa gaaatggacc ggttgccgtt
      841 cgcgccgcgg cgctacgctt ctgccacatc ggttgcgctg gcacgcaaac tggcggagat
      901 cgcgccgggg cgcttgtcca ggatgctgtt caccaccggc gggtcggacg ccatcgaagt
      961 ggcggtgaag atcgctcgca cggcgaccgg ccgccacaag accctatcgt tctgggatgc
     1021 cttccacggc gccggtttcg gcgcctccgc cctgtcgggg gaggccttgt tccgctccgg
     1081 acgcgccgcg ccgttgatcg ccggggacca gcacgttgcc ccctttgcct gctatcgctg
     1141 tgcctacggg cacgattgcg atgccagtgg ccggccgcgg ctcgaagtgt gcaagctgtc
     1201 ctgcgcgcgc atggtggact acgtgctttc acgcgaaacc gacgtggcct gtgtcgtggc
     1261 ggaacctgcg cgcgccgttc cgtatgtgcc gccgccgggg ttctggcagg cagttcgcga
     1321 ggcctgcgat cgccacggaa cgctgctggt cttcgatgaa atcccgacgg gtctcggcaa
     1381 gacgggccgc atgtttgctt gcgatcacga cggcgtcgaa ccggacatac tggtcatggg
     1441 caaggcgctc ggcggcggca tcctgcccat cgcggcggtg atcacgagac ccgacctcga
     1501 tgtcggcggt gactacgcct tcgggcacta tacccacgag aaaaacccgg tgacggcccg
     1561 tgcggcgctg accaccatcg agatcatcga ggatgaaggc ctggtggaga atgcccgccg
     1621 tgtcggcgat gccgcgctgg aacggctcga cgccatgaaa cgccgcctgc ccgccatcgg
     1681 cgacgtgcgc ggacgtggcc tgctgctggg aatagacctg gtcgccgaca gggacagcaa
     1741 ggcgcccgcg ggcgacctgg cggaagcggt gctgtatcgg gcgctcgatg ccggcctttc
     1801 gttcaagacc acgatgggca acgtactgac tctgaccccg ccgctgaccg taagcacgga
     1861 ccagatgatg gccgcgctgg atatcattga gacagcgatc acggcgggat aggcccttgc
     1921 gcggcaggga gcgttgtcct gcctgttact tgacgcagag cggcggaggc gtagctttcg
     1981 cccatgggcg caggagttcg tcccatcctg atgcagatgc ggtgctggtc cgcctgcctg
     2041 ctgttgtgcc tttcgtcacc cgctctttct ctggaacggc cgcaagcagg cgcaggcacc
     2101 ggcctgccct gcacatgccg cgcccttggg cgttcctgga acctgggcga agaaatctgc
     2161 atgccgacgg cgcagggtcc gcgcttggca gtatgcgaca tggttctcaa caacacgtcc
     2221 tggcgcacca cgaaccaacc ttgcccgcag gcaagtctga ggccgacctt gtcacccgga
     2281 tagggattac tccgcctgcg ccagagcctg ttcgaggaag tccgcagcgg ccagagccat
     2341 caggtcctcg ccgaagccgg ccaccacctg cacgccgacc ggcagcccgt cggcggtttc
     2401 gtagcccggc acgttcaggg ccggggtgcc caggagggtg aaaagcttgt tgaaggtcgc
     2461 gacacccgta gtcgaccggt cgggtgcaac gcccggggcg gcacaggtga ggagaacatc
     2521 gacgtcctcg aacagcgcct tggactgccg ccttgcggcc tttgccgcgc ctcgtgccgc
     2581 gtcgtattcc gcgacggagg caggccgtac cgggttcatg aaggagcgga actcttccga
     2641 cagtgcgtcg gcatgatggt cgagctccca gcgcgcggcg agcgcgccct cgaactggta
     2701 gatcgtggcg tgggcatcct gggccgcagc gaatgtgggc ggggcagcaa tcgacacgac
     2761 gttcgcgccg gcattctcgg cggcgcggat ggcgaatcgc aaggccgcct gcgactccgg
     2821 ttccggctcg cccgcgaacg tcatgtccag cacaccgaac gtcggcgtgc cgaagtcgcg
     2881 gccgtcaacg cgcaggtcgc gtccggaaac ggcgcccagc ccgaaagcga cgtcgcgcac
     2941 acgcgccccg aacagcccga gcgtgtccat ggtccaggaa ccggccttga tcccgaccat
     3001 gggcaacagc cggaacgacg gcttgatcgc cgcgacgccg caatagctcg ccgggcggat
     3061 gacggatcct ccggtctgcg tgccaaaggc gagcggcatc atgcccgcag cgacgccggc
     3121 cgcggagccg gaagaggaac cgcccggtgt ccgcgccgga tcatgcggat tgcgcgtcgg
     3181 cgagggatgc agaaatgcga gttcggtcgt cacggccttg ccaagaacga gaccgccggc
     3241 ggcctgggtc atggagacga tggtcgcgtc ggccttcggc tgccatccgc gccagaccgg
     3301 cgaaccgtat tcggtcggca gtccggcggt gtcgatgatg tccttgaagg caaccggcat
     3361 cccgtgcagc gggccggatt gcgggcgcag cgcggccgcg cggagagcat caaggtcgag
     3421 gtgcgcgaag gcgccgatgt cggcctcgcg ggcggcaatc gcctccgcga tcagttctac
     3481 gaccccggac accgcgagat cacccgcatc caaggcttcg gccagatcca gcgccctgag
     3541 cataccccac tcctcacgtc ggcgtcccgg acgcgcgatc ctgtcacagg tagtgccgtt
     3601 cttgggccgg ggcaatcggt gtcgtcacgt cgaaggggca gcggaacttc gctttgcgag
     3661 gtcggcgggc tggccgcccg gagccactcg accgccaaca agcccgacgc ctttccagac
     3721 ttgccttcgc gggccttttg cgcctataag ccggcacttc gctgaaccgc ccggctggta
     3781 agggctgccg tggcggttct ttgtttgctc gattttcgag tgattttgcg gccttcgggc
     3841 tgtctggaga gcaaaatgcc caagttgaag accaaatcgg gcgccaagaa gcgcttcaag
     3901 attaccgcca ccggcaaggt ga
//
LOCUS       DAOMXN010000226         3907 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_12647_length_3907_cov_12.142004, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000226 DAOMXN010000000
VERSION     DAOMXN010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12647_length_3907_cov_12.142004"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="PK812_12470"
     CDS             <1..895
                     /locus_tag="PK812_12470"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRJ70407.1"
                     /translation="GRIMRLGMTAEPIRPDLSTLTAALDECEALGIDFVELSTYDMDL
                     VVGGAIRREQLGRVKAHLRGRRLRWSVHGPLAINLFDQERIARHRAVLNASLEVAAEL
                     GARNYVMHAGILYEVSTAAEVLAARRRQIDELGRIGERARALGVVVCVENLFLGETRK
                     HTPTPSEIAADLSAVNHASVRATLDFSHAHQQATQLGLDVVRECAALAPFAEHLHIHD
                     SFGLKDDIWMYSRGEALAFGHGDLHMPPGWGDVPWGRLLDACRFPAECWGNIELDHRY
                     WHEREACVAATREAFAAMLAQ"
     gene            complement(900..2675)
                     /gene="xsc"
                     /locus_tag="PK812_12475"
     CDS             complement(900..2675)
                     /gene="xsc"
                     /locus_tag="PK812_12475"
                     /EC_number="2.3.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050487 - sulfoacetaldehyde
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0019529 - taurine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoacetaldehyde acetyltransferase"
                     /protein_id="HRJ70408.1"
                     /translation="MRMTTEEAFVKVLQMHGIEHAFGIIGSAFMPISDLFPKAGITFW
                     DVAHETNGGLMCDGYTRSTGKMAMAIAQNGPGITGFVTAVKTAYWNHTPMLLVTPQAA
                     NKTIGQGGFQEVEQMALFRDMVCYQEEVRDPTRIAEVLNRVISKARRLSAPAQINIPR
                     DYWCQIVDIALPSIIDFERPAGGANAIRQAASLLSEAKFPVILSGAGVVIGGAIPECV
                     ALAERLDAPVCSNYQHNDSFPGSHRLACGPLGYNGSKAAMEIIAKADVVLALGTRLNP
                     FSTLPGYGIDYWPKTAKIIQVDINPDRIGLTKPVSVGICGDAKQVAAQILAQLSKTAG
                     DAGRDERKALVHKTKSAWLQQLSSMDHEDDDPGTSWNDRARKRDPERMSPRQAWRAIQ
                     AGLPREAIISSDIGNNCAIGNAYPTFEQGRKYLAPGLFGPCGYGFPSIIGAKIGNPDT
                     PVVGFAGDGAFGISMSEMSSIGRKEWPGITMVIFRNYQWGAEKRNTTLWYDDNFVGTE
                     LDPHLSYAKVAEGCGLKGVSVRTQADLTRELAQACEAQKQGVTTFIEVILNQELGEPF
                     RRDAMKKPVVVAGIDPKDMRPQKGS"
     gene            2870..3736
                     /gene="purU"
                     /locus_tag="PK812_12480"
     CDS             2870..3736
                     /gene="purU"
                     /locus_tag="PK812_12480"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007606018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HRJ70409.1"
                     /translation="MSPSSPLVLKLSCVDRPGIVADVSRFLAENGGNILEAQQFDDAE
                     TSRFFMRVVFALTDARATPGELRAGFETVAQRWTMTWTLRERDEQRRVMLLVSKMDHC
                     LVDLLYRRRIGELPMQVTAIVSNHPAEHYARNDFGDIPFHHLPITRETKMEQEAALWQ
                     LIRDTRSEVVVLARYMQVLSDGLTAKLAGRCINIHHSFLPGFKGARPYHQAHARGVKL
                     IGATAHYVTSDLDEGPIIEQDVERISHADTPDDLVRKGRDIERRVLARALRYHLEDRV
                     MINDGKTVVFMS"
BASE COUNT          619 a         1322 c         1289 g          677 t
ORIGIN      
        1 cggtcggatc atgcggcttg gaatgacggc ggaaccgatc cgcccggacc tgtcgaccct
       61 gacggcggcc ctcgacgagt gcgaggcgct cggcatcgat ttcgtcgaac tgtcgaccta
      121 cgacatggac ctggtcgtcg gcggcgcgat ccggcgcgag cagcttggcc gggtgaaggc
      181 gcacttgcgg ggccggcgcc tgcgctggtc ggtgcacgga ccgctcgcca tcaacctgtt
      241 cgaccaggaa cgcatcgccc gccaccgggc ggttctgaac gcaagtctgg aggtcgcggc
      301 cgagcttggc gcacgcaact acgtgatgca cgccggcatc ctctacgagg tctcgacggc
      361 ggccgaggtc ctcgccgccc gccgccgcca gatcgacgaa ctcggccgga ttggcgaacg
      421 cgcgcgggcg ctgggcgtcg tggtctgcgt cgagaacctg ttcctcggcg aaacccgcaa
      481 gcacacgccg acgccctcgg agatcgcggc ggacctgtcc gccgtgaacc acgcatccgt
      541 ccgcgcgacc ctcgacttca gccacgccca ccagcaggcg acccagctcg gcctcgacgt
      601 ggtgagggaa tgcgccgcgc tcgcgccctt cgccgagcac ctgcacatcc acgattcctt
      661 cggcctgaag gacgacatct ggatgtattc gcgcggcgag gcgctggcct tcggccacgg
      721 cgacctgcac atgccgccgg gctggggcga cgtgccctgg ggacggctgc tcgacgcctg
      781 ccgcttcccg gcggaatgct ggggcaacat cgagctggac caccgctatt ggcacgagcg
      841 ggaggcctgc gtcgccgcga cgcgcgaggc gttcgcggcg atgctcgcgc aatagggcgt
      901 cagctgccct tctgcgggcg catgtccttc ggatcgatcc cggcgacgac gaccggcttc
      961 ttcatggcgt cgcggcggaa cggctcgccc agttcctggt tgaggatgac ctcgatgaac
     1021 gtggtgacgc cctgcttctg ggcctcgcag gcctgcgcca gttcgcgcgt caggtccgcc
     1081 tgcgtgcgca cgctgacgcc tttcagcccg cagccttcgg ccaccttggc gtaggacagg
     1141 tgcggatcga gttccgtgcc gacgaagttg tcgtcgtacc agagcgtggt gttccgcttt
     1201 tccgcgcccc actggtagtt gcggaagatc accatggtga tgcccggcca ttccttgcgg
     1261 ccgatcgagc tcatctcgct catggaaatg ccgaaggcgc catcgccggc aaagcccacg
     1321 accggcgtgt ccggattgcc gatcttcgcg ccgatgatcg aggggaaccc gtagccgcag
     1381 ggcccgaaca ggcccggggc gagatatttc cggccctgct cgaaggtcgg ataggcattg
     1441 ccgatggcgc agttgttgcc gatgtcggag gaaatgatcg cctcgcgcgg cagtccggcc
     1501 tgaatggcgc gccacgcctg gcgcggcgac atgcgctcgg gatcgcgttt gcgggctcgg
     1561 tcgttccagc tggtgccggg gtcatcgtcc tcgtgatcca tggacgagag ctgctggagc
     1621 caggcggatt tcgtcttgtg caccagggcc ttgcgctcgt cgcgcccggc atcgcccgcc
     1681 gtcttggaca actgggcgag gatctgcgcc gccacctgtt tcgcatcgcc gcagatgccg
     1741 acgctcaccg gcttggtcag gccgatgcgg tccgggttga tgtcgacctg gatgatcttc
     1801 gcggtcttcg gccagtagtc gatgccgtag cccggcaggg tcgagaacgg gttgagccgg
     1861 gtgccgagcg ccagcaccac atcggccttg gcgatgatct ccatcgccgc cttcgaaccg
     1921 ttgtaaccga gcgggccgca ggcaagccgg tggctgccgg ggaaggaatc attgtgctgg
     1981 tagttggagc agaccggcgc gtccagccgc tcggccagcg ccacgcattc cgggatcgcc
     2041 ccgccgatga ccacgcccgc tcccgacagg atgaccggga acttcgcctc cgacagcagg
     2101 ctcgccgcct gccggatggc gttcgcgccg ccggccggcc gctcgaaatc gatgatcgag
     2161 ggcagggcga tgtcgacgat ctggcaccag tagtcgcgcg ggatgttgat ctgcgccggc
     2221 gccgacaggc gcctggcctt ggaaatgacc cggttcagca cctcggcgat gcgcgtggga
     2281 tcgcgcacct cttcctggta gcagaccatg tcgcggaaca gggccatctg ctccacctcc
     2341 tggaatccgc cctgcccgat ggtcttgttg gccgcctgcg gcgtgaccag cagcatgggc
     2401 gtatggttcc agtaggctgt cttcaccgcc gtgacgaagc cggtgattcc cggaccgttc
     2461 tgggcgatcg ccatggccat cttgccggtc gagcgggtgt agccgtcgca catcagcccg
     2521 ccattggtct cgtgcgccac atcccagaag gtgatcccgg ccttcggaaa caggtcggaa
     2581 atcggcatga aggcggaacc aatgataccg aatgcgtgct cgatgccgtg catctgcagc
     2641 accttcacga aggcttcctc agtcgtcatg cgcatcggcg cgtctccctc tttttggctt
     2701 tttgcgtcgc gcatgcgtat gcaaacggtc cgtggcccaa gactagaaag cctgcaccac
     2761 gaaaggcaag ggctgaaaac cctgtcctga agaaaatatc aacctatgct gccgaggctg
     2821 cgggcggccg catcgccgtt tgttgacgat gcccaggagg tgacccccga tgtccccaag
     2881 ctcgcctctc gtgctcaagc tttcctgcgt cgacaggccc ggcatcgtcg ccgacgtgtc
     2941 gcgctttctg gccgagaacg gcggcaacat cctggaggcg cagcagttcg acgatgccga
     3001 gaccagtcgc ttcttcatgc gggtcgtctt tgccctgacg gacgcccgcg cgacccctgg
     3061 cgaattgcgc gccggattcg agacggtggc gcagcgctgg accatgacct ggaccctgcg
     3121 cgagcgcgac gagcagcgcc gcgtcatgct tctggtgtcg aagatggacc attgccttgt
     3181 cgatctgctc taccgccggc gcatcggcga actgccgatg caggtgaccg ccatcgtctc
     3241 caatcacccg gccgagcact atgcccgcaa cgacttcggc gacatcccgt tccatcatct
     3301 gccgatcacg cgcgaaacca agatggagca ggaagcggcg ctgtggcagc tgatccggga
     3361 cacgcggagc gaggtggtcg tgctcgcccg ctacatgcag gtgctttcgg acggcctgac
     3421 cgccaagctc gcgggccgct gcatcaacat ccaccattcc ttcctgcccg gcttcaaggg
     3481 cgcgcggccc tatcaccagg cgcacgcccg cggcgtgaag ctgatcggcg ccacggccca
     3541 ttacgtcacc tcggacctcg atgaagggcc gatcatcgag caggatgtcg aacgcatcag
     3601 ccatgccgac acgccggacg atctcgtgcg caagggccgc gacatcgagc gccgggtgct
     3661 ggccagggcg ctgcgctacc atctggaaga ccgcgtgatg atcaacgacg gcaagaccgt
     3721 cgtgttcatg agctgagccg ccgtgcgccg gtcgctgtct cccgatctcg ttctcgtcgt
     3781 aaacgccggc tcgtcctcgc tgaagctggc gctgatgccg gcggaacgcg gcagcgcggg
     3841 ctggcgggcg caggtcgagc gtatcggcgc aagcgcctcg gccgagatcc gggacggcca
     3901 cgggtcc
//
LOCUS       DAOMXN010000227         3879 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_12781_length_3879_cov_9.420241, whole genome shotgun sequence.
ACCESSION   DAOMXN010000227 DAOMXN010000000
VERSION     DAOMXN010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12781_length_3879_cov_9.420241"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="PK812_12485"
     CDS             complement(<1..829)
                     /locus_tag="PK812_12485"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HRJ70410.1"
                     /translation="MILVCGEALVDLFLEPAQGAAIPARVIAGGSPFNVAVGLGRLGA
                     RAAFLGGLSSDHFGQHLAALLDTEGVSLDFARHLPQLTTISVVALGPGGTPRYAFHGE
                     GAADRMFGVADLPGELPPGIAAITFGSYSLAVEPCGSAYLALAQREKDRRVISLDPNV
                     RPTIIGDMARWHNRFATFAACASVIKASEEDIVLAYGETAAIGEIARMWLGFGPRLVV
                     ITRGADGAVAFAGSDSLALPGHAVDVVDTVGAGDTFHAALLARLDQRGWLDRDAIGAL
                     "
     gene            955..3135
                     /gene="glgB"
                     /locus_tag="PK812_12490"
     CDS             955..3135
                     /gene="glgB"
                     /locus_tag="PK812_12490"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007770210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HRJ70411.1"
                     /translation="MSVGKLSDLDILALVTGTHADPFSVLGAHRRGRETILRAFVPGA
                     RSLSATDLKGGAIASLRRIHDAGLFEARVAARGGAPIRYEAVGDAGPWAFVDAYSFGP
                     VLGPMDDYFAAEGSHLRLFDKLGAHVLTHQGVSGVHFGVWAPNARRVSVVGDFNQWDG
                     RRHIMRLRQGSGIWEIFVPDIGEGALYKFELIGADGRLALKSDPFAFAGELRPKNASV
                     VARIDGFAWTDGAYLEARAHRDQRRTPISIYEVHAGSWRRPHGGFPTWEQLAGTLIPY
                     VRDSGFTHIEFLPVSEHPLDASWGYQPTGLFAPTARFGTPEDFARFVDAAHAAGIGVI
                     LDWVPAHFPTDAHGLAHFDGTALYEHADPRQGFHPDWNTAIYNFGRREVANFLINNAL
                     FWLEKYHIDGLRVDAVASMLYLDYSRKEGEWVANRQGGRENLEAIDFLKALNRTVYAA
                     HPSVLMIAEESTSFPGVSQPVHAGGLGFGFKWNMGFMHDTLRYLMRDPVHRRHHHADL
                     TFGLLYAFSENFVLALSHDEVVHGKASLLHKMAGDDWQKFATLRAYYAFMWGYPGKKL
                     LFMGQEFGQRREWSEDRELDWEALDHAPHAGTARSIRDLNRLYRSLPALHARDCEGEG
                     FRWLVVDDAARSVVAFARFGGAGTQPAVIVSNFTPVPRKGYVLPMPAAGRWREMFNSD
                     ASDYGGSGIGNLGMVQAEPEGIADQPARARINLPPLATLILAKD"
     gene            3138..3398
                     /locus_tag="PK812_12495"
     CDS             3138..3398
                     /locus_tag="PK812_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70412.1"
                     /translation="MPLLFAPIPVILGLIGMLIYATIYSLAESVFVLGLCALIGSACY
                     VVSKALLNVATRIDRMEVKFLAGALAVLTTMTSIIYFVSDNT"
     gene            3636..>3879
                     /locus_tag="PK812_12500"
     CDS             3636..>3879
                     /locus_tag="PK812_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70413.1"
                     /translation="MFDAKQLLNALISGAGQQNQQQQGQPNQPAGGLGGLLGAVLSQV
                     QNTAQQAGQASQQAGGVGGILGSILKQATSGVGQAAG"
BASE COUNT          616 a         1281 c         1317 g          665 t
ORIGIN      
        1 ggagcgcgcc gatggcatcg cggtccagcc atccccgctg gtccagccgg gccaaaaggg
       61 cggcatggaa tgtgtcgccg gcgccgaccg tgtcgaccac gtcgacggcg tggccgggca
      121 gtgccaggct gtcgctgccg gcgaaggcga cggcgccgtc ggctccccgc gtgatcacca
      181 cgagccgcgg cccgaagccg agccacatcc gcgcgatctc gccgatggcg gccgtttcgc
      241 cataggccag gacgatgtcc tcctcgctgg ccttgatcac gctcgcgcag gcggcgaacg
      301 tggcgaagcg gttgtgccac cgggccatgt cgccgatgat cgtcgggcgc acattcggat
      361 cgaggctgat gacgcgacgg tccttctcgc gctgcgccag ggccagatag gcggagccgc
      421 agggttcgac agccagagag tacgatccga acgtgatggc ggcgatcccg ggcggcagct
      481 cgcccggcag gtcggcgacg ccgaacatgc gatccgccgc gccctcgcca tgaaaggcgt
      541 agcggggggt gccgccgggg ccgagagcga cgacggaaat cgtcgtcagc tgcggcagat
      601 ggcgggcaaa atcgagcgaa acgccctccg tgtcgagcag ggcggccaga tgctggccga
      661 aatggtcgct ggacagtccc ccgaggaatg ccgccctggc gcccaggcgg ccaaggccga
      721 ccgcgacatt gaacggcgat ccgccggcga tcacgcgggc cgggatggcc gcgccctgcg
      781 ccggctccag gaacaggtcg accagcgcct cgccgcaaac cagaatcatc gtcttgcctc
      841 cgccaggcag ggtgagcctg cacgtagcca tacaaaaggt gttcgcgtag cggaagcaac
      901 cgctatgctg cgcggggtgc ttcgtggcgg cggagcgagg cttggagtgg cggcgtgtct
      961 gtcggaaagc tatcggatct cgatatcctt gccctggtga cgggcacgca tgccgatccg
     1021 ttctccgtgc tcggcgccca caggcgcggc cgagagacga tcttgcgcgc cttcgttcct
     1081 ggcgcgcggt cgctctcggc caccgacctg aaggggggcg cgatcgcgtc gctccggcgc
     1141 atccacgatg ccggactgtt cgaggcgcgg gtcgcggcgc gcggcggcgc tccgatccgt
     1201 tacgaggccg tcggggatgc cggcccctgg gccttcgtgg atgcctacag ctttggcccg
     1261 gttctcggcc cgatggacga ttacttcgcc gcggaaggct cgcatctgcg gctgttcgac
     1321 aagctcggcg cccatgtcct gacgcatcag ggggtcagcg gcgtgcattt cggcgtctgg
     1381 gcgccgaacg cgcggcgggt ctcggtcgtc ggcgatttca accagtggga cggacgccgc
     1441 cacatcatgc ggttgcggca gggctccggc atctgggaga tcttcgtgcc cgacatcggg
     1501 gagggcgctc tttacaagtt cgaactgatc ggcgccgacg ggcggctggc gctcaagagc
     1561 gatccctttg ccttcgccgg tgagctgcga ccgaagaacg cctcggtggt ggcgcggatc
     1621 gacggcttcg cctggaccga cggcgcctat ctggaagccc gcgcgcaccg cgatcagcgc
     1681 cgcacgccga tctcgatcta cgaggtccat gcggggtcct ggcgccggcc tcacggcgga
     1741 ttcccgacct gggagcagct ggccgggacg ctgatcccgt atgtccgcga ctccggcttc
     1801 acccacattg agttcctgcc ggtgtccgag catccgctcg atgcctcctg gggctaccag
     1861 ccgaccggcc tgttcgcgcc gaccgcgcgg ttcggcacgc cggaggactt cgcccgtttc
     1921 gttgatgcgg cacatgccgc cggcatcggc gtgatcctcg actgggtgcc ggcgcatttt
     1981 cccaccgacg cgcacggcct ggcgcatttc gacgggaccg cgctctatga gcatgccgat
     2041 ccgcgccagg gcttccatcc ggactggaac accgcgatct acaatttcgg acgccgggag
     2101 gtcgcgaact tcctgatcaa caacgcgctg ttctggctgg agaaatacca catcgacggg
     2161 ttgcgggtgg atgccgtggc ctcgatgctc tacctcgatt attcgcgcaa ggagggcgag
     2221 tgggtcgcga accggcaggg tggacgcgag aacctcgaag ccatcgactt tctcaaggcg
     2281 ctgaaccgca cggtctacgc cgcccatccc tcggtgctga tgatcgcgga ggaatccacc
     2341 tcgttcccgg gcgtgtcgca gccggtgcat gccggtggcc tcggcttcgg cttcaagtgg
     2401 aacatgggct tcatgcacga tacgctgcgc tacctgatgc gcgatcccgt gcatcgcagg
     2461 caccaccacg ccgacctgac cttcggcctg ctctacgcct tcagcgagaa tttcgtgctg
     2521 gcgctgtcgc atgacgaggt ggtgcacgga aaggcgtcgc tgctgcacaa gatggcgggg
     2581 gacgactggc agaagttcgc gacgcttcgg gcctattacg ccttcatgtg gggctatccc
     2641 ggcaagaagc tgctgttcat ggggcaggaa ttcggccagc gccgcgagtg gagcgaggac
     2701 cgcgagctgg attgggaggc gctggaccat gcgccgcatg ccgggaccgc ccggagcata
     2761 cgcgatctga accggctgta tcgcagcctg ccggcgctgc atgcgcgcga ctgcgagggg
     2821 gagggcttcc gctggctggt ggtcgacgac gcggcgcgct cggtcgtggc cttcgcccgc
     2881 ttcggcggcg cgggcacgca gccggcggta atcgtcagca acttcacgcc ggtgccgcgc
     2941 aagggctacg ttctgcccat gccggccgcc gggcgctggc gcgaaatgtt caattccgac
     3001 gcgtccgact atggcggctc agggattggt aatcttggca tggtacaggc agagccagag
     3061 gggattgccg accaaccggc ccgcgcgcgg atcaatctgc cgccgctggc cacgctgata
     3121 ctggccaagg actgaccatg cccctccttt tcgcgcccat tccggtcatt ctcggcctga
     3181 tcggcatgct gatctacgcc accatctatt cgctggccga atccgtgttc gtactgggtc
     3241 tctgcgccct gatcggttcg gcctgctatg tcgtctcgaa ggctctgctg aacgtcgcga
     3301 cccggatcga ccgcatggag gtcaagtttc tggcgggcgc gctggcggta ctgaccacga
     3361 tgacatcgat catctatttc gtgtccgaca acacctgata ccaggcttca tggcccgtga
     3421 ccttcgggtc ctgatttcct gcactttcat ttcccattga aagacaagtg aaatttcaag
     3481 agctgtcatc gccccgtctc gtgagcgatg gtatggagca gcccttgcgg ccccggtcat
     3541 tgaaaccttg gcgccgcgct ccaatattcg gcgggcgctc atccggcgtt cagcggccgc
     3601 aggcaatacg cgcgttgcca aatcaggggt tcaccatgtt cgacgcaaaa cagcttctca
     3661 atgcgctcat ctccggcgcc gggcagcaga atcaacagca gcagggccag ccgaaccagc
     3721 cggcaggagg cctcggaggc ctgctcggcg ctgttctcag ccaggtccag aacacggcgc
     3781 agcaggccgg tcaggccagc cagcaggccg ggggcgtcgg cggcatcctg gggtcgatcc
     3841 tcaagcaggc gacctccggc gtggggcagg cggccggcc
//
LOCUS       DAOMXN010000228         3840 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_12998_length_3840_cov_12.104095, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000228 DAOMXN010000000
VERSION     DAOMXN010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12998_length_3840_cov_12.104095"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="PK812_12505"
     CDS             <1..770
                     /locus_tag="PK812_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70414.1"
                     /translation="ANAAAARAQGRAPGWYALIPLAGPYMSTAGAPWRTREAEARVAL
                     EGQLAGLLFAGALYLLGVAFAAPMLHKAALIGFLFNLVQLVPLRPFDGGRLFPGLDWP
                     ATRDEDVSARLWLSLAFERLVRRIGCRIGLDDASRPAALRSLIGPALFTIMLAGFAAV
                     AVVAVLLPPKAIALAILLWVAGTLALILFVLCLDPRVLRSGDERLLAPRRPLEPLPEV
                     HRGPVPWSSRTKARCALLAYLLLLAAHALGALASWTA"
     gene            complement(795..1430)
                     /locus_tag="PK812_12510"
     CDS             complement(795..1430)
                     /locus_tag="PK812_12510"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007010659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HRJ70415.1"
                     /translation="MTATGPHPLPQRLIEGYSTFRHNRLPRERERFEEMARDGQKPEI
                     MVIACCDSRVSPEVIFDARPGELFVVRNIANLVPPFAPDNGLHGVSAALEFAVQALKV
                     KHIVVMGHGRCGGVHAFVRRLRHPTASDALSPGDFIGRWMTLLSPAAEALPGQNGEDD
                     FDYAERLARHSIRNSIENLLTFPCINILHSKGRLQLHGAVFDVTTGVLEAL"
     gene            1584..2465
                     /locus_tag="PK812_12515"
     CDS             1584..2465
                     /locus_tag="PK812_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007010729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HRJ70416.1"
                     /translation="MLTFDPHRPRRSVLYVPASNERAMAKASGLAADTIILDLEDAVA
                     PDQKARARQSVLAMLRSGQFIGTEVVVRVNGLATQEIAADVAVAVHADAVLVPKIDSA
                     LDIARCEAALDASAPDRRVGLWAMIETPRAVLSIAEICAEAERPAARLSCLVVGTNDL
                     AKDARVAIVPGRAPLLSWMSQCVLAARAYGLVVLDGVFNDVRDADGFAAECRQGRELG
                     FDGKTLIHPAQIGPANALYGPSEAEIAAASAVVEAFARPENAGKGVIVVDGRMTERLH
                     LDMAQRVLAGASAYGAR"
     gene            complement(2462..>3840)
                     /locus_tag="PK812_12520"
     CDS             complement(2462..>3840)
                     /locus_tag="PK812_12520"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HRJ70417.1"
                     /translation="AGAAQGAVAQPGSAISATVEPGDSLSAIAERHGLALDQLVALNP
                     QLQGRDLNLIHPGETFTVGRVPDLNPDPEGLLPLPKAPVAANPDPEGLLPLPKAPAAA
                     SPDPEGLLPLGALPDPTRVDIGPIGPEGLTQAQKDQLKDQAIQLIGAMRGYGTLGAGA
                     LDGSSGWRELPVTFQGLGTAIYDQTVLDDALKSLESGDWGKFVAHDLPALNKAWTTTM
                     NSLQTNGLKNAGAYADTFASANKLQALGNIGYILAVHDGGWGQVSHDSATATVDALKK
                     LSTGAQYDTIVNKVLEKEALQGTKLGAMRMTPETSAKLQNWMLGLTATLDVMDKIAKM
                     NAISEGDPATQVAKTGYANALTANDYGIGQYLEDIKEGAPEFAKFIVDNPEVGAQIAT
                     ELGINAATSYALNQVIVGLAQRAGGTVTAGSVLNSIISRLSTPFMFFYVDPEMMSRLT
                     GPSESDLA"
BASE COUNT          606 a         1281 c         1255 g          698 t
ORIGIN      
        1 gggcgaatgc cgcggccgcc cgcgcgcagg gtcgcgcgcc cggctggtat gcgctgatcc
       61 cgctcgccgg accctacatg tcgacggccg gcgcgccctg gcgcacccgc gaggccgagg
      121 cgcgggtggc gctggagggc caactcgcgg gactgctgtt cgccggcgcc ctgtacctgc
      181 tcggcgtggc attcgccgca ccgatgctgc acaaggccgc cctgatcggc ttcctgttca
      241 atctggttca actcgtgccc ctgcgtccgt ttgacggcgg gcgactgttt cccggactgg
      301 actggcccgc gacccgggac gaggacgtca gtgcccggct gtggctgtcg ctggcgttcg
      361 aacgcctggt gcgccgcatc ggttgccgga tcgggctgga tgacgcaagc cgaccggcgg
      421 cgctgcgcag cctgatcgga ccggccctgt tcacgatcat gctcgccggc tttgccgctg
      481 tcgccgtcgt tgccgtgctg cttccgccca aggcgattgc cctggccatc ctgctatggg
      541 ttgcgggcac gctcgccctg atcctgttcg tgctctgcct cgatccgcgc gtgctgcgca
      601 gcggcgacga acgcctgctg gcaccgcgcc ggccgctgga gccgctgcct gaggtacatc
      661 gcggaccggt gccctggtcc tcgcgtacca aggcgcgctg cgccctactg gcctatctcc
      721 tgctcctggc cgcccacgcg ctcggggccc ttgccagctg gaccgcctga acgctgccgg
      781 acgccccgta aactctaaag cgcctcgagc acacctgtcg tcacgtcgaa gacggcgccg
      841 tgcagttgca gcctgccctt gctgtgcagg atgttgatgc agggaaaggt cagcagattt
      901 tcgatggaat tgcggatgga atggcgggcc aaccgttcgg cgtagtcgaa gtcatcctca
      961 ccattctggc ctggaagcgc ctcggcggcc ggtgacagca gcgtcatcca acggccgatg
     1021 aaatcgcccg gcgacagcgc atccgaagcg gtggggtggc gcaagcggcg aacgaaggca
     1081 tgcacaccgc cacagcggcc atgacccatg accacgatgt gcttgacctt gagcgcctgc
     1141 accgcgaatt ccagcgccgc cgaaacgcca tgcagcccat tgtccggcgc gaagggcgga
     1201 acgaggttcg cgatgttgcg caccacgaac aactctcccg gacgggcatc aaatatgact
     1261 tccggcgaga cgcgcgagtc acagcaggca atgaccatga tctcgggctt ctggccatcg
     1321 cgcgccattt cttcgaaccg ttcgcgctcg cgcggcagtc gattgtggcg aaaggtgctg
     1381 taaccttcga tcagccgttg cggcaacgga tgtggaccgg tggcagtcat cactacttca
     1441 ggcccatgcc tgactccccg cttgttgcac tgcaacgtag ggctcggcca ccagacgaca
     1501 agaccaccat tcgggtaagc gcacccggac gcgcgacagc gctccatctt gcagcaggcc
     1561 gccgcctcgc gctatttcgt gccatgctga cattcgaccc gcaccgaccc cggcgcagtg
     1621 ttctctacgt tcccgcctcg aacgagcgcg ccatggcgaa ggcgtctggc ctcgccgcag
     1681 acaccatcat cctcgatctg gaggacgcgg tggcgcccga tcagaaggcg agggcgcgcc
     1741 aatctgtcct cgccatgctg cgctccgggc agttcatcgg cacggaagtt gtcgtgcgcg
     1801 tcaacggcct ggcgacgcag gagatagcgg cagacgtggc ggttgcggtg catgccgacg
     1861 ccgtgctcgt gccgaagatc gactcggcgc tcgacattgc ccgctgcgag gcggcgctgg
     1921 acgccagcgc tccggatcgg cgcgttggcc tctgggccat gatcgaaacg ccgcgcgcgg
     1981 tgctctccat cgccgaaatc tgcgctgaag cggaacgtcc cgcggcgcgg ctgtcctgtc
     2041 tggtggtcgg cacgaacgac cttgccaagg atgcgcgagt cgcgatcgtt ccagggcgcg
     2101 cgccgctgct gtcctggatg agccagtgcg ttctggcggc ccgcgcctac ggactcgtgg
     2161 tgctggatgg cgtcttcaac gacgtgcgcg acgcggacgg tttcgccgct gagtgccgcc
     2221 agggccggga gctcggcttt gacggcaaga cattgatcca cccggcccag atcgggcccg
     2281 ccaacgccct ttacggcccg tccgaggccg agatcgccgc tgccagcgcc gttgtggaag
     2341 ccttcgcgcg gccagagaac gccggcaaag gcgtgatcgt tgtcgacggc cgcatgaccg
     2401 agcggctgca tctggacatg gcgcaacggg tgctggcggg cgcctccgca tacggcgcgc
     2461 gctaggcaag gtccgattcg ctcgggcctg tcagacggct catcatctcg gggtcgacgt
     2521 agaagaacat gaagggtgtc gacagccggg aaatgatcga attcagcacg gagccggccg
     2581 ttacggtccc gcccgccctc tgggccagac cgacaatgac ctgattgagt gcgtaggacg
     2641 tcgcagcatt gatacccagt tccgtcgcga tctgcgcgcc gacctcgggg ttgtcgacaa
     2701 tgaacttggc gaactccggc gcgccttcct tgatgtcttc caggtactgg ccgatgccat
     2761 agtcattggc tgtgagcgcg ttggcgtagc ccgtcttggc cacctgggtc gcgggatcac
     2821 cctccgagat cgcgttcatc ttggcgatct tgtccatcac gtcgagcgta gcggtcaagc
     2881 cgagcatcca gttctggagc ttggccgacg tttccggcgt catgcgcatg gcgccgagct
     2941 tcgtgccttg caacgcctcc ttttccagga ccttgttgac gatcgtgtcg tactgggcgc
     3001 cggtcgacag cttcttgagt gcatccaccg tggcggtggc gctgtcgtga ctgacctggc
     3061 cccagccgcc atcgtgaacg gcgaggatgt agccaatgtt gccaagcgcc tgcaacttgt
     3121 tcgccgacgc gaacgtatcg gcataggcgc cggcattttt cagcccattc gtttgcaggg
     3181 agttcatcgt agttgtccag gccttgttca gggctggcag gtcatgggcg acgaactttc
     3241 cccaatcgcc ggattccaga gatttcaacg catcatcgag aaccgtctga tcgtatatgg
     3301 ccgttcccag tccctgaaac gtgaccggca actcgcgcca ccccgaagaa ccatccaaag
     3361 cgccggcacc gagcgtgccg tagccgcgca tggcgccgat cagctggatc gcctggtcct
     3421 tcaactggtc cttctgggcc tgtgtgaggc cttccggacc gatgggtccg atgtcgacac
     3481 gcgtcggatc cggcaaggct ccgagcggga gaagaccctc ggggtcagga cttgcggcgg
     3541 cgggcgcctt cggcagcggc agaagccctt cggggtccgg gtttgcggcg accggcgctt
     3601 tcggcagagg caagagacct tccgggtccg ggttcaggtc gggaaccctg cccacggtga
     3661 atgtctcgcc gggatgaatg agattaaggt cccgtccttg aagctgcggg ttaagtgcaa
     3721 ccagttggtc gagcgcaaga ccatggcgct ctgcgatcgc ggagagactg tcacctggct
     3781 cgaccgtcgc ggaaatcgcg ctccccggct gcgccaccgc gccctgcgcc gcgcccgctg
//
LOCUS       DAOMXN010000229         3831 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_13046_length_3831_cov_12.344015, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000229 DAOMXN010000000
VERSION     DAOMXN010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13046_length_3831_cov_12.344015"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="PK812_12525"
     CDS             <1..732
                     /locus_tag="PK812_12525"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ70418.1"
                     /translation="PATAPARPADVAELMKASPYKGPERLRQAAVNGNLAAIYEIGAR
                     YADGRGTARDLKLASRWLEFAAEQGFAPAQYRFGSFNREGLGMQQDAKVAFQWFMKAA
                     EQGHILAMHNLAVLYAEGVNGSPDYASAAAWFRNAAEHGVKDSQFNIAILHVRGLGVV
                     QDMVEAYKWFAIAAQKGDADAIKRRDEVAARLSPNQLEDAKNRVEEFRPRRPDMRAND
                     VGAPEGGWDQAPRAPGAPQQKKSRV"
     gene            813..2006
                     /locus_tag="PK812_12530"
     CDS             813..2006
                     /locus_tag="PK812_12530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRJ70419.1"
                     /translation="MQVRPLKGREVGSVTSGDAVAPLRVLCVTPAGPEGMGGIDRLYH
                     YMREHMGTGGGAPDISIRYFASRGPRDGYRSFLGFPFRLLAFVWVLLRSRADIVHLNH
                     STHGSAVRKWALALVARLFGCRVTTHFHGMITPEDYAANPFWLGLFRSLCGMSDRIVV
                     LGDVFTRWFRDTLGVAPERLVVIPNGIPDFAPDLALPKPAGPDRLILFAGEVGPRKGA
                     GLLLEALAMVRARLGDWRCVMAGNGDIHEYRAMTQMLGLKDRVRFTGWVGPDAVHALM
                     REAVVVVLPSRVEALPLSLIEGACAGCALIASNAGASAEIARDGVNGRIVPLEAEAIA
                     DVLTEVLSSPDLLARMQESSRALYVERFRIEAFEAALLAMFRSLAPPSREAARLLTPS
                     PDPAP"
     gene            complement(1978..3237)
                     /locus_tag="PK812_12535"
     CDS             complement(1978..3237)
                     /locus_tag="PK812_12535"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HRJ70420.1"
                     /translation="MSITLSRRTLLAGMTAALTAPVSASEPRAPFRRGASIHNMMNWA
                     SVQEGDKTRYRSPPFATPAHQLLPGVLANLKRAGLDFIRLTLDPGPFLQFTGNERDDL
                     DRQLLAAIARLSEAGFGVVVDFHPNWQNPTYAPEKITALSDGPLTKAYGQMLARTARL
                     LKPLSGGRIAFELMNEPQWGWDAPSTTRWQVIQKTWHDQVRAEAPDLMLVLTGARGGD
                     REGLLAMDGLRYAGSNVLWSFHYYKPYLLTHQGVKGDNEMSRAWRHFSDLPYPAGPEH
                     HDDAWRIIRRNIETSAEVKPQDRAAVLAQARKALKDYLDLKPGRALVAREFAEVAEWA
                     RRNGVPAQRIFLGEFGITRTYGPYRASPPQTLENYLSDVRGEAEAQGFGWAIWAVSGY
                     GGMSLIREDDKPDLDEPTVRALGLATA"
     gene            complement(3237..>3831)
                     /locus_tag="PK812_12540"
     CDS             complement(3237..>3831)
                     /locus_tag="PK812_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70421.1"
                     /translation="ETYICACLIGVLMLTFTPMGAASLEGRLRLKALIVLGLLAAGPI
                     LLLIADSAGFLERFYGGYFDENAKARVDVYKVFDWVTTDEILFGTDLIRIREMVFDRL
                     GLLIESSVVVFVFQFGLFGAILLAGTIIWTLLRAAWGPEPRAFLAVGAFLATASSNDA
                     MSGKHSYFALLIVMLLAFRNEARDAWGRAKTLRWETR"
BASE COUNT          612 a         1272 c         1294 g          653 t
ORIGIN      
        1 ccggcgaccg ccccggccag gccggccgat gtggccgaac tgatgaaggc atcgccctac
       61 aagggtccgg aacggctgag gcaggccgcc gtcaacggca atctcgcggc gatctacgag
      121 atcggcgcgc gctatgccga cggccgtggc accgcccgcg acctcaagct cgcgtcgagg
      181 tggttggaat ttgcggccga gcaggggttt gctcccgctc aataccggtt cggcagtttc
      241 aatcgcgaag gccttggtat gcagcaggac gccaaggttg ccttccagtg gttcatgaag
      301 gcagccgagc agggtcatat cctggcgatg cataaccttg cggtgctcta tgcggagggc
      361 gtcaacggca gcccggatta tgcctcggct gccgcctggt tccgcaacgc cgccgagcac
      421 ggggtcaagg acagccagtt caacatcgcc atcctgcatg ttcgtggcct gggcgtcgtg
      481 caggacatgg tcgaagccta caagtggttc gccatcgccg cccagaaggg cgacgccgac
      541 gccatcaagc gtcgtgacga ggtggcggca cggcttagcc cgaaccagct ggaggacgcc
      601 aagaaccgcg tcgaggagtt ccgtccccgg cggccggaca tgcgggccaa cgacgtcggc
      661 gcgccggaag gcggctggga ccaggcgccc cgcgccccgg gtgcgcccca gcagaagaag
      721 tcccgggtgt aaggcagacc tccttaccgc gcgcgaaagc cgacgggtcg ggacgtgtcc
      781 gcataacccg gccttaagcc ggatatgctg caatgcaggt tcggcccctc aagggccggg
      841 aggttggatc ggtgacatcc ggcgatgctg tcgcgcccct gcgcgttctg tgcgtgaccc
      901 cggcggggcc ggaaggcatg ggtggcatcg atcgcctgta ccattacatg cgcgagcaca
      961 tggggaccgg cggcggtgcg ccggacattt cgatccgcta cttcgcctcg cgcgggccgc
     1021 gcgacgggta tcgctccttc ctcggctttc cattccgtct gctggctttc gtctgggttc
     1081 tgctgcgcag ccgtgccgac atcgtgcacc tcaatcactc cacccatggc agcgccgtgc
     1141 gcaaatgggc tcttgcgctg gtggcgcggc tgttcggttg ccgcgtgacg acgcacttcc
     1201 atggcatgat cacgcccgag gactatgccg cgaacccgtt ctggcttggt cttttccgtt
     1261 cgctctgcgg catgtcagac cgcatcgtcg tcctgggcga tgtgttcacg cgctggttcc
     1321 gcgataccct gggcgtggcg ccggaacgcc tggtggtcat ccccaatgga atccccgact
     1381 ttgcgccgga tcttgctctc cccaagccgg cggggccgga tcgcctgatc cttttcgccg
     1441 gcgaggtcgg tccgcgcaag ggcgcggggc tcctgctgga agcgctggcc atggtgcgtg
     1501 cccgcctagg ggactggcgc tgcgttatgg ccggcaacgg cgacattcac gaataccgcg
     1561 ccatgacgca gatgctcggt ctgaaggatc gggttcgctt caccggctgg gtcggtcccg
     1621 acgcggtgca cgcgctcatg cgcgaggctg tggttgtggt gctgccctcg cgcgtcgagg
     1681 ccctgccgct gtcgctgatc gaaggggctt gcgccggctg cgcgctgatt gcctcgaatg
     1741 ccggcgccag tgccgagatc gcgcgtgacg gggtcaacgg gcgcatcgtc ccgctggaag
     1801 ccgaggcgat cgccgatgtc ctgaccgagg tgctgtccag ccccgacttg ctcgcacgga
     1861 tgcaggagtc gagccgcgcg ctctatgtcg aacgcttccg aatcgaggcg ttcgaggcgg
     1921 cgctgctcgc catgttccgt tcgcttgcgc cgccttcgcg tgaggcggct cgcctgctca
     1981 cgccgtcgcc agacccagcg ccctgacggt cggctcgtcg agatccggtt tgtcgtcctc
     2041 gcggatcagc gacatcccgc cgtagcccga gacagcccag atcgcccagc cgaagccctg
     2101 cgcctcggct tcgccgcgca cgtcggagag ataattctcc agcgtctgcg gcggagaagc
     2161 ccgatagggt ccgtaagtgc gggtaatccc gaactcgcca aggaagatgc gctgcgccgg
     2221 cacgccgttg cgccgcgccc actccgccac ctcggcgaat tccctggcga caagcgcgcg
     2281 gcccggtttc aggtccaggt agtccttcag tgccttgcgc gcctgagcaa gcacggcggc
     2341 ccggtcctgc ggtttgacct cggcgctcgt ctcgatgttc cggcggatga tgcgccaggc
     2401 gtcgtcgtgg tgctccggcc ccgcgggata gggcaggtcc gagaaatggc gccaggcgcg
     2461 cgacatctcg ttgtcgccct tcacgccctg atgcgtcagc agatacggct tgtagtagtg
     2521 gaagctccac agcacgttgg agccggcata gcgcagaccg tccatcgcca gcagcccttc
     2581 ccggtcgccg ccgcgtgctc ccgtgagcac cagcatcaga tccggcgcct cggcgcgcac
     2641 ctgatcatgc caggtcttct ggatcacctg ccagcgcgtt gtggagggtg cgtcccagcc
     2701 ccattgcggt tcgttcatca gctcgaaggc gatgcgcccg ccgctcaacg gcttcagcag
     2761 gcgcgcggtc cgcgccagca tctggccata ggccttcgtc agcggaccat cggagagggc
     2821 ggtgatcttc tctggcgcat aggtcggatt ctgccagttc gggtggaaat ccacgacgac
     2881 cccgaaaccc gcctcggaca gtcgggcgat ggcagccagc agctggcggt caagatcgtc
     2941 ccgttcgttc cccgtgaact gcaggaacgg tcccgggtcc agcgtcaggc ggatgaagtc
     3001 cagaccggcc cgcttcaggt tcgccaggac gccgggcagg agctgatgcg ctggtgtcgc
     3061 gaagggcggc gagcgatagc gggtcttgtc gccctcctgc accgaggccc agttcatcat
     3121 gttgtggatg ctcgcgcccc ggcggaaagg cgcgcgcggc tcgctggccg aaaccggcgc
     3181 cgtcagcgcg gccgtcattc cggcaagaag cgtgcgtcgg gacagggtaa tggacatcag
     3241 cgggtctccc agcgcagggt tttggcccgg ccccaggcgt cgcgcgcctc gttgcggaag
     3301 gccagcagca tgacgatcaa cagggcgaag taggaatgct ttccggacat ggcgtcattg
     3361 ctggacgctg tcgcgagaaa ggccccgacg gcgaggaagg cgcgcggctc cggcccccag
     3421 gcggcgcgca gcagcgtcca gatgatggtt cccgccagca ggatcgcccc gaacaggccg
     3481 aactggaaga cgaagaccac gaccgagctc tcgatcagca ggccaagccg gtcgaacacc
     3541 atttcgcgga tgcggatcag gtccgttccg aacaggatct catcggtggt gacccagtcg
     3601 aacaccttgt agacatcgac gcgcgccttg gcgttttcgt cgaaataccc gccgtagaat
     3661 cgctccagga acccggcgga atcggcgatg agcagaagga tcggaccggc tgcgagcagg
     3721 ccgagcacga tcagcgcctt gaggcgcagg cgtccctcca gtgaggccgc gcccatcggc
     3781 gtgaaggtga gcatcaagac gccgatcagg caggcgcaga tgtaggtctc c
//
LOCUS       DAOMXN010000230         3789 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_13244_length_3789_cov_8.402250, whole genome shotgun sequence.
ACCESSION   DAOMXN010000230 DAOMXN010000000
VERSION     DAOMXN010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13244_length_3789_cov_8.402250"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..102
                     /locus_tag="PK812_12545"
     CDS             <1..102
                     /locus_tag="PK812_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008124821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRJ70422.1"
                     /translation="MLDWDAPTGGYLLTACFATGIAAAEGVKSFLDR"
     gene            complement(105..3050)
                     /locus_tag="PK812_12550"
     CDS             complement(105..3050)
                     /locus_tag="PK812_12550"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HRJ70423.1"
                     /translation="MMEKTYDPASIESRVAKAWTDADAFAAGRPGRENAPAYCIVIPP
                     PNVTGSLHMGHALNNTLQDILCRYERMRGKDVLWQPGTDHAGIATQMVVERKLAQEKR
                     PGRRELGREKFLEEVWAWKAQSGGTIVNQLKRLGASCDWKRERFTMDEGLSKAVIEFF
                     VALHEKGLIYKDKRLVNWDPKLLTAISDLEVEQVEIKGSFAWARGQVDKEGNPVALDH
                     AALGKLLDKQPGGHLYYFEYPVVDDAGRETGEVITVATTRPETMLGDTGVAVHPEDER
                     YKRLHGKRVRLPLVGRLIPIVADEYSDPEKGTGAVKITPAHDFNDFEVGKRQGLAKVN
                     ILTGTAAIYLARNNEPNPDFADVLNGSDHKLLEVLLTLDGLDRFDARKRVVALMDAAG
                     RLAKIEPTTQQVPHGDRSGVPVEPFLTDQWYVNVKPLADRSMEAVRSGKTQMLPPKSE
                     ADFFRWMENIEPWCISRQLWWGHQIPAWYGPQADGSGPFDAERFVAKNATEALALAQQ
                     FYGRGKTVCISDRDSDSNGAMEFHCRIDSVDRGEIHIWRDRDVLDTWFSSALWPFSTL
                     GWPEDTRELQKFYPNSVLVTGFDILFFWVARMMMAGLFTQDEVPFRAVYLHGLVRDEK
                     GRKMSKTIGNVIDPLVVIDQYGADALRFTLASLAAQGRDPKLGVRTAEGYRNFCTKLW
                     NASRFAEMNGCARVPGFDPKSVKGTLNRWVLSELAKALAAVEEGIGAYRFNEAASAAY
                     RFVWNTFCDWYLELAKPALQGADGAEKDEARATTAFCLDQIVKILHPFMPFITEELWT
                     AYSERAGEKPELLCLAQWPVLSGLEAPEAEAEIGWIVELITDIRSARAEMNVPAASQI
                     PLVLVGADAAVRTRVGDWAEVLKRLARLSEIAFADTAPGQSIQLVVRGTSAALPLAGI
                     IDIAAEKLRLAKELDKTAQDIARIDAKLGNADFVARAPEEVVEEQRERREEGEARKAK
                     IAEALARLG"
     gene            complement(3170..3553)
                     /locus_tag="PK812_12555"
     CDS             complement(3170..3553)
                     /locus_tag="PK812_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009494578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4260 domain-containing protein"
                     /protein_id="HRJ70424.1"
                     /translation="MTETRSAMRLGLRIEALAVFCAATALFWRLDGSWTFYLIFFFTP
                     DVALAGYLVGNRAGAWAYNAAHSTVGPLALGIAAMVFDSSMAVALALIWLAHIGFDRA
                     LGYGLKYESGFKDTHLGRIGGNSPA"
     gene            complement(3558..>3789)
                     /locus_tag="PK812_12560"
     CDS             complement(3558..>3789)
                     /locus_tag="PK812_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2497 domain-containing protein"
                     /protein_id="HRJ70425.1"
                     /translation="AEPDPARLISDQTAGTTAKAFQSLANAVFTQNARTMDDVVADML
                     RPMLKSWLDENLPTLVERLVKAEIERVARGGR"
BASE COUNT          659 a         1234 c         1202 g          694 t
ORIGIN      
        1 atgctggact gggacgcgcc gaccggcggc tacctgctca ccgcctgttt cgcgaccggc
       61 atcgcggcgg cggaaggggt gaagagcttc ctcgatcgct gagcctaccc cagccttgcc
      121 agcgcctcgg cgatcttggc cttgcgggcc tcgccttcct cgcggcgctc gcgctgctcc
      181 tccaccacct cttccggcgc gcgggcaacg aagtcggcat tgccgagctt ggcgtcgatg
      241 cgggcgatat cctgcgccgt cttgtccagt tccttggcca gccgcagctt ttccgccgcg
      301 atgtcgatga tcccggccag cggcagcgcc gccgaggtgc cgcgcaccac gagctggatc
      361 gactggcccg gagcggtgtc agcgaaggca atctccgaca gccgcgccag gcgcttcagc
      421 acctcggccc agtcgccgac gcgggtgcgg acagccgcat ccgccccgac caggaccagc
      481 ggaatctggc ttgccgccgg cacgttcatt tcggcgcggg ccgagcggat gtcggtgatc
      541 agctcgacga tccagccgat ctcggcctcc gcctcgggcg cttcgaggcc ggaaagcacg
      601 ggccattgcg ccaaacacag caactccggc ttctcccccg cccgctccga gtaggccgtc
      661 cacaactcct ccgtgataaa cggcatgaac ggatgcagga tcttgacgat ctggtcgaga
      721 cagaaggccg tggtcgcccg ggcctcgtcc ttctccgcgc cgtccgcacc ctggagcgcc
      781 ggcttggcga gttcgagata ccagtcgcag aaggtgttcc agacgaagcg gtaggccgcg
      841 ctggcggcct cgttgaagcg ataggcgcca atgccctcct ccaccgccgc cagcgccttg
      901 gcgagttcgc tcaggaccca gcggttgagc gtgcccttca cggacttcgg gtcaaagccg
      961 ggaacgcgcg cgcagccgtt catttcggcg aagcgcgagg cgttccaaag cttggtgcag
     1021 aagttgcgat agccttccgc tgtgcgcacg ccgagtttcg gatcgcgccc ctgcgcggcg
     1081 agcgaggcca gcgtgaagcg cagcgcgtcg gccccgtact ggtcgatcac caccagcgga
     1141 tcgatgacgt tgccgatggt cttcgacatc ttccggccct tctcgtcgcg gacgaggccg
     1201 tgcagataga cggcgcggaa cggcacctcg tcctgcgtga acaggccggc catcatcatg
     1261 cgcgccaccc agaagaacag gatgtcgaag ccggtgacga gcacggaatt gggatagaat
     1321 ttctggagtt ccctggtgtc ttccggccag ccgagggtcg agaacggcca cagggcggag
     1381 gaaaaccagg tgtcgaggac gtctcgatct cgccagatat gaatttcgcc gcggtcgaca
     1441 ctatcgatcc tgcaatgaaa ttccatcgcg ccgttggagt ctgaatccct atctgagata
     1501 caaacggttt tgcctcggcc atagaattgc tgggccaatg caagtgcctc ggtagcattc
     1561 tttgctacaa accgttccgc atcaaatggc ccggatccat ctgcttgtgg cccgtaccat
     1621 gccgggatct ggtggcccca ccagagctgg cgcgaaatgc accacggctc gatgttctcc
     1681 atccagcgga agaaatcggc ctcgcttttg ggcggcagca tctgggtctt gccggagcga
     1741 accgcttcca tcgagcggtc ggccaacggc ttgacgttga cataccactg gtcggtcagg
     1801 aacggctcga ccggtacgcc ggagcgatca ccatgcggca cctgctgcgt ggtcggctcg
     1861 attttggcga gacgcccggc cgcgtccatc aaagcgacga cacgctttcg ggcatcaaag
     1921 cgatcaagac catcaagcgt cagtagaact tctaaaagct tgtggtcact accatttagg
     1981 acatctgcaa agtcaggatt gggctcattg ttgcgggcca gatagatggc tgccgtgccg
     2041 gtcagaatat tgaccttcgc cagcccctgc cgcttgccca cctcgaaatc gttgaaatca
     2101 tgcgccggag tgatcttcac cgcgccggtg cccttttccg ggtccgaata ttcatctgcg
     2161 acgatgggga tcagccggcc gaccagcggc agccgcacac gcttgccgtg caggcgcttg
     2221 tagcgttcgt cctcggggtg cacggcgacg ccggtgtcgc ccagcatggt ctcgggccgc
     2281 gtggtggcca cagtgatgac ctcgccggtt tcccggccgg catcatccac cacgggatat
     2341 tcgaaatagt agagatggcc gccgggctgc ttgtccagca gcttgccgag agcggcatgg
     2401 tcgagggcga ccggattgcc ctctttgtcc acctgcccgc gcgcccaggc gaagctgccc
     2461 ttgatctcga cctgctcgac ctcaaggtcc gagatggcgg tgagcagttt cgggtcccag
     2521 ttcacaaggc gcttgtcctt gtagatcagg cccttttcgt gcagcgccac gaagaattcg
     2581 atgacggcct tggacaggcc ctcgtccatg gtgaagcgct cgcgcttcca gtcgcaggag
     2641 gcgccgaggc gcttcaactg gttgacgatg gtcccgccgg actgcgcctt ccaggcccag
     2701 acctcttcga ggaatttttc gcggcccagt tcgcgccttc ccggcctttt ctcctgcgcc
     2761 agcttgcgct ccaccaccat ctgcgtggcg atgccggcgt gatcggtgcc gggctgccag
     2821 agcacgtcct tgccgcgcat gcgctcgtag cggcagagga tatcctgcaa cgtgttgttg
     2881 agcgcatggc ccatgtgcag cgagccggtg acgttcggcg gcgggatgac gatgcaatag
     2941 gccggcgcgt tctcgcggcc gggacgaccg gcggcgaaag cgtcggcgtc ggtccaggcc
     3001 ttggcgacgc gcgattcaat cgaggccgga tcgtaggtct tttccatcat ggcggcaggt
     3061 ccgtaggcgc gaggcgcgcg aattcttcaa agcgcttcaa tcccttcgcc gcgccgcgat
     3121 gtcaacgggt tcagcgatca ggaggtcccg gactccgcat ctgacgggtt caggccggcg
     3181 aatttccgcc gatccgccca agatgcgtat ccttgaagcc gctctcgtac ttcagtccgt
     3241 acccgagcgc gcgatcgaag ccgatatgcg ccagccagat caacgccagt gcaacagcca
     3301 ttgatgagtc gaagaccatg gcagcgatgc ccaaggcaag cggaccgacg gtcgagtggg
     3361 ccgcgttgta ggcccaggcc ccggcacgat tgccaacgag gtagcccgcc agggcgacat
     3421 cgggagtaaa aaagaagatc aggtagaagg tccagcttcc gtcgaggcgc cagaacagcg
     3481 ccgtcgcggc acagaacacc gccagagctt caatgcgcag gcccaagcgc atcgcgctcc
     3541 gggtttcggt catgacgcta gcgtccgccg cgcgcgacgc gctcgatctc ggccttgacc
     3601 agccgttcga cgagggtggg caggttctcg tccagccagg acttgagcat cggacgcagc
     3661 atgtcggcga cgacgtcatc catggttcgg gcgttctggg tgaacaccgc gttggccaga
     3721 gactggaacg ccttggcggt tgtgcccgcg gtctgatcgg agatcaggcg ggccgggtcg
     3781 ggctctgcg
//
LOCUS       DAOMXN010000231         3747 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_13436_length_3747_cov_10.062026, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000231 DAOMXN010000000
VERSION     DAOMXN010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13436_length_3747_cov_10.062026"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..835)
                     /locus_tag="PK812_12565"
     CDS             complement(131..835)
                     /locus_tag="PK812_12565"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HRJ70426.1"
                     /translation="MGVSTPADRHAGVDKATGAADSADLDTALARFVLDLRSRGLRDL
                     GLLAAFEKTPRAAFLPKHLAPLTYQPFALPIDCGGEATAPQMVATLLGALHLRPGMKV
                     LDVGTGSGFQAAVLARYGCKVVTIERFRTLHLAAGRAMLRQQVVNVRLEHGDGTAGFE
                     AEAPYDRILVNAAMKDVPDALVEQLAPGGSLAIPIQQGRDQKLLVFDDMGETYRQRDL
                     GHCTFQAMVAGIPLVL"
     gene            complement(838..1605)
                     /gene="surE"
                     /locus_tag="PK812_12570"
     CDS             complement(838..1605)
                     /gene="surE"
                     /locus_tag="PK812_12570"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HRJ70427.1"
                     /translation="MRILITNDDGIHADGLAALEQIAAQLTDDVWVIAPESDQSGVAH
                     SLSLNDPLRLRKISDKRFAVKGTPTDCVIMAVRHVMAEHRPDLVLSGINRGHNVAEDV
                     TYSGTIAGAMEGTILGIPSIALSQGYPHRDRDAIRYDYAVKEAPALIRRVLEIGIDPG
                     ILVNINFPGVEPSEVKGVKVAVQGQRDQTALLNIVKRGDGRGLPYYWLAFNPVQFEPK
                     EGTDLHAVREGYISITPLRLDLTDMPSVTRFAEAFEG"
     gene            complement(1605..2888)
                     /gene="serS"
                     /locus_tag="PK812_12575"
     CDS             complement(1605..2888)
                     /gene="serS"
                     /locus_tag="PK812_12575"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRJ70428.1"
                     /translation="MHDIRFIRENAAAFDQALKNRGLAPLSEALLSLDEKRRVAIAAH
                     ETALARRNAASKEIGQAMAQKDAVRAEALKAEVAALKDAIAGKQAEADVADAELRKQL
                     EIIPNLPLKSVPVGADETGNVEVRRHLEPPTFNFAPKQHFELGEALGLMDFEAAAKIS
                     GARFVVLKGALARLERALGQFMLDLHTGQHGYEEIIAPLMVKDEAVYGVGQLPKFSED
                     MFHTENGFWLIPTSEVSMTNLVREEIVDEATLPRRYTALTPCFRSEAGSAGRDTRGML
                     RQHQFMKVELVSITTPETSLAEHERMTGCAETVLKKLGIPFRTVVLCTGDMGFASQKT
                     YDIEAWLPGQGTYREISSCSVCGDFQARRMNARCKTKDSKGTRFVHTLNGSGIAVGRA
                     LIAVMENYQNADGSITVPEALRPYMGGLEKITKAN"
     gene            complement(2976..>3747)
                     /locus_tag="PK812_12580"
     CDS             complement(2976..>3747)
                     /locus_tag="PK812_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HRJ70429.1"
                     /translation="GGAGGTLGAGIAAKAAPDGYTFFMGAAHHAIAPGLYPKLEYDLE
                     KDFIPIGVLAAPPQVLVAHPEKIKARTLADFLAHVRANPGQVTYASAGNGTTHHLAGE
                     LFKLTTKTDLRHVPYRGAGPAMQDLLAGVVDVEFDGLGSSAAQIAGKRLVALAVASPK
                     RNPAIPDVPTFAEAGIAGYEVATWYALWAPKGTPQPVIDRVTDELKKALASEPIKAAW
                     AKNGSEIPNLWGADFGKFVTAEIARWGKVVREANVKLD"
BASE COUNT          642 a         1247 c         1193 g          665 t
ORIGIN      
        1 aacgacgatc tgataccatc ggtcatcagg gatgaccgat gaccgctctt gaattatcct
       61 tgtcttacaa cgcgcaaggt aaattcaaga aaggaaccaa gggtccattg tcatgaccct
      121 tggtatgagg tcagagcacc agggggatgc ccgccaccat cgcctgaaag gtgcagtgcc
      181 cgagatcgcg ctggcgatag gtctcgccca tatcgtcgaa caccagcagc ttctggtcgc
      241 ggccctgctg gatagggatt gccaggctgc caccgggcgc cagttgctcc accagcgcgt
      301 ccggcacgtc cttcatcgcg gcgttgacca ggatgcggtc gtagggggct tcggcctcga
      361 accccgctgt tccgtcgcca tgctcgagcc tgacattgac gacctgctgg cgcagcatcg
      421 cgcggccggc ggccagatgc agcgtccgga accgctcgat cgtcaccacc ttgcagccat
      481 agcgcgccag cacggcggcc tggaacccgg agccggtgcc aacgtcgagc accttcatgc
      541 cgggccgcag gtggagcgca cccaggagcg tcgccaccat ctgcggcgcg gtcgcctcgc
      601 cgccacagtc gatcggcagc gcgaagggct gataggtcag cggcgccaga tgcttgggca
      661 gaaaggcggc gcgcggcgtc ttctcgaagg ccgcgaggag gccgagatcg cgcaatcccc
      721 gcgaacgcag gtccagcacg aaccgcgcca gcgcggtatc gagatcggcg gaatcggcgg
      781 cgccggtcgc cttgtcgaca ccggcatgtc ggtcagcggg cgtcgaaacg cccacgatca
      841 tccttcaaac gcctcggcga accgggtgac gctcggcatg tccgtcagat cgaggcgcaa
      901 cggcgtgatc gagatatagc cctcgcgcac cgcatgcagg tccgtgccct ccttgggctc
      961 gaactgcacc ggattgaagg cgagccagta gtatggcagg ccgcgcccgt cgccgcgctt
     1021 gacgatgttc agcagcgcgg tctggtcgcg ctggccctgc acggccacct tgacgccctt
     1081 gacttccgaa ggctccacac cggggaagtt gatgttgacc aggatgcccg ggtcaatgcc
     1141 gatctccaga acgcggcgga tcagtgccgg cgcttccttc acggcgtagt cgtagcggat
     1201 ggcatcgcgg tcgcggtgcg gatagccctg gctcagcgcg atcgacggaa tgcccaggat
     1261 ggtgccttcc atcgccccgg cgatcgtgcc ggaataggtg acgtcctccg cgacattgtg
     1321 accccggttt atgccggaga gcacgagatc cggccgatgc tcggccatga catggcgcac
     1381 ggccatgatg acgcaatcgg tcggcgttcc cttcacggcg aagcgcttgt ccgaaatctt
     1441 gcgcaggcgc agcggatcgt tgagcgacag ggaatgcgcc acgccgctct ggtcggattc
     1501 aggcgcgatg acccatacgt cgtccgtgag ctgggcggcg atctgctcca gcgccgcaag
     1561 cccgtcggcg tgaataccgt cgtcgttggt aatgaggatg cgcatcagtt cgctttcgta
     1621 attttttcaa gaccgcccat atacgggcgc agcgcctccg gcacggtgat cgagccatcg
     1681 gcgttctggt agttttccat caccgcgatc agcgcccggc caaccgcaat tcccgagccg
     1741 ttgagcgtat gcacgaatcg cgtgcctttc gagtctttcg tcttgcagcg cgcgttcatg
     1801 cgccgggcct ggaaatcgcc gcagaccgaa caggaggaaa tctcgcgata ggtgccctgc
     1861 cccggcagcc aggcctcgat gtcgtaggtc ttctgcgagg caaaccccat gtcgccggtg
     1921 cacagcacca cggtgcggaa cggaatgccg agctttttca gcacggtttc ggcgcaaccc
     1981 gtcatgcgct cgtgctcggc gagcgaggtc tccggcgtgg tgatggagac aagctccacc
     2041 ttcatgaact ggtgctggcg cagcatgccg cgcgtgtcac ggcccgccga tccggcttcc
     2101 gagcggaagc agggggtgag cgcggtatag cggcgcggga gcgtcgcctc atcgacgatc
     2161 tcctcccgca cgaggttggt catcgagact tcggacgtcg ggatgagcca gaaaccattc
     2221 tcggtatgga acatatcctc tgaaaatttc ggcaattgac cgacgccgta gactgcctcg
     2281 tccttcacca tcagcggcgc gatgatctcc tcatacccat gctgccccgt gtgcaggtcc
     2341 agcatgaact ggccgagcgc ccgctcaagc cgcgccagcg cgcccttcaa aaccacgaag
     2401 cgcgcgccgg aaatcttcgc cgccgcctcg aaatccatca ggccgagcgc ctcacccagt
     2461 tcaaaatgct gtttgggcgc gaaattgaac gtcggcggct ccagatggcg acgcacttcc
     2521 acattgccgg tttcatccgc gccgaccggc acgcttttga gcggcaggtt ggggatgatc
     2581 tcaagctgct tgcgcagttc tgcgtcggcg acatcggcct cggcctgttt gccggcgatg
     2641 gcatccttca gcgccgcgac ctcggccttg agggcctcgg cccggaccgc atccttctgc
     2701 gccatggcct gcccgatctc tttcgaggcg gcgttgcggc gggcgagcgc ggtctcgtgc
     2761 gcggcgatcg cgacgcggcg cttttcatcc agcgacagca aggcctccga caggggcgca
     2821 aggccccggt tcttcagggc ctgatcgaag gcggcggcgt tctcgcggat gaaacgtatg
     2881 tcgtgcatgg ctcaggctgc gacgcgggta tttcgggatg cgcccttaga aacccgcgcc
     2941 cgcccggatg caagcggcat tttgaccctc aggcctcaat ccagcttgac gttcgcctcc
     3001 ctgaccacct tgccccagcg agcgatttcg gccgtgacga actttccgaa gtcggcaccc
     3061 cagagattgg gaatctcgga accattcttg gcccaggccg ccttgatcgg ctccgacgcg
     3121 agcgccttct tcagctcgtc cgtgacccgg tcgatcaccg gctgcggcgt gcccttgggc
     3181 gcccacaacg cgtaccaggt ggccacctcg tatccggcga taccagcctc cgcgaaggtc
     3241 ggaacgtcgg gaatcgccgg attgcgcttg ggcgaggcca cggccagcgc caccaggcgc
     3301 ttgccggcga tctgcgccgc cgaggagccg agcccgtcga actccacgtc gacgacaccc
     3361 gcgagcagat cctgcatggc cggtccggcg ccgcgatacg gcacgtggcg caggtcggtc
     3421 ttggtggtca gcttgaacag ttcgcccgcc aggtgatgcg tcgtgccgtt gccggcggag
     3481 gcataggtca cctgccccgg attggccctc acatgcgcga ggaagtccgc gagcgtgcgc
     3541 gccttgatct tttcagggtg ggccaccagc acttgcggcg gcgccgcaag cacgccgatc
     3601 ggaatgaaat ccttctccag gtcgtattcg agtttcggat acagccccgg cgcgatcgcg
     3661 tgatgcgccg cgcccatgaa gaaggtgtat ccgtccggag cggccttcgc ggcaatgccg
     3721 gcaccgagcg tgccgccggc cccgcca
//
LOCUS       DAOMXN010000232         3695 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_13697_length_3695_cov_10.344231, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000232 DAOMXN010000000
VERSION     DAOMXN010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13697_length_3695_cov_10.344231"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(178..672)
                     /locus_tag="PK812_12585"
     CDS             complement(178..672)
                     /locus_tag="PK812_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HRJ70430.1"
                     /translation="MAKKPVETTAQTAAETVKQAFAPITEAVQNIQNSIQVPEAARDF
                     VKKAATAGEQRAAALNANVEKATDSVESAAVAVVNEIATVSRNVQKAMHEDVAAYFAN
                     LGKLATAKCPSEAMQIQADYLRGRGEVAVSRVKAASAYVGEKVKSGVKSAQDNLAQFG
                     KTAA"
     gene            complement(828..1424)
                     /locus_tag="PK812_12590"
     CDS             complement(828..1424)
                     /locus_tag="PK812_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRJ70431.1"
                     /translation="MSLGDIKTYPRKAQIEGGEIELRLMGAKDEKAVAAFAKALPVHD
                     LLFLPRDITQAKVLKAWIAESERGGMTSLIAVKGKEVVGCAAIVTDPLSWSPHVGEIR
                     VVTSSSVRGEGVGRVLTHAAFSLALSSGLEKLVVQMTVDQKGGIALFEGLGFKAEALL
                     RDHVRDKSGKKHDIVVLGHDVAKVWSQMQAFGLPSAVG"
     gene            complement(1421..2503)
                     /locus_tag="PK812_12595"
     CDS             complement(1421..2503)
                     /locus_tag="PK812_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRJ70432.1"
                     /translation="MSSAASGPAGDITSRVQEEVQRAIQRSIKGVEYFASSAPPVGVT
                     PKDVIYARGPMNLYHYRPLTDEIYRIPLLIVMATTNRGYVLDMAPGQSFVEYLLKRGY
                     DVFMLDWNAPKPDEKRLAFEDYVLDFIPTAVKCVQEDTGEKDVNICGYCFGGVLSLLY
                     AATHAGGPLKNLVCFTTPVDFREMKLFQNFSSQKHFDVDHLVDSAGNVPPELIFTSFE
                     MLRPASRAVSQVHLWENIWNDEYVKSYRMFDRWATDTLPLAGEYFRQTTKRLMWENAL
                     YNGTMTVGGRKADLARIKAPILHVVAEHDHIIPYGASRPLIEKIGSTDKEEVMLKGGH
                     ISVVAGPNAVKRLWPKLDNWLGGRSV"
     gene            complement(2500..2880)
                     /locus_tag="PK812_12600"
     CDS             complement(2500..2880)
                     /locus_tag="PK812_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(R)-hydroxyalkanoic acid synthase subunit
                     PhaE"
                     /protein_id="HRJ70433.1"
                     /translation="MSEKPIDPMAMFNQMLGQFEQGFNEMATKAMGSEDFSRIMNTMG
                     GASMGAKKTMGDMMERYLATMNLPSRQELTNIGERLQAVEARLNEIIAILHRAHPDVG
                     GLVKPTAAPRPPRTRKPPPAGSKA"
     gene            3010..>3695
                     /locus_tag="PK812_12605"
     CDS             3010..>3695
                     /locus_tag="PK812_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRJ70434.1"
                     /translation="MATVARTDETFATANGVSLCWQAFGEESAEPLVLIMGLGAQMVM
                     WEDPFCEQLAARGFRVIRFDNRDIGRSQKMTGGERVTFADLIGLSLAGKPIPAVYTLK
                     DMAADTVGLLDALGIARAHIVGLSMGGAIAQEIALGWPDRVKTLTSIMASPGGAGLPP
                     PTPEAAAVLMTPPPATKEEYVATFQRTWKVLRAGSFPQDEATDADRAERNWSRGLNPA
                     GVGRQLVAIFA"
BASE COUNT          631 a         1230 c         1140 g          694 t
ORIGIN      
        1 ttgatgcgtc gcagctcccg cgctttctcg agatcgccgg cgccggacgc cggacctggc
       61 aaggaacgct gggtcatgcc gctccccctc acttcggcag atatgctacc gcagcggcca
      121 aaaaagaagg cccgcacgcg gcgggccttc cgggctatca ggcgatcagg atgctgatca
      181 ggccgccgtc ttgccgaact gggcgaggtt gtcctgcgcc gacttcacgc cggacttcac
      241 cttctcgccg acataggccg acgcggcctt gacgcgggaa acggcaacct cgccgcgacc
      301 acgcaggtag tcggcctgga tctgcatcgc ttccgacggg cacttcgcgg tcgcgagctt
      361 gccgaggttg gcgaagtagg cagccacgtc ctcgtgcatc gccttctgga cgttgcgcga
      421 cacggtggcg atctcgttga cgacggcaac ggcggcggac tcgaccgagt cggtggcctt
      481 ttcgacattg gcgttcaggg cggcagcgcg ctgctcaccg gcggtcgcgg ccttcttcac
      541 gaagtcgcgg gcggcttccg gaacctgaat ggaattctgg atgttctgaa ccgcttcggt
      601 gatcggcgcg aacgcctgct tcacggtttc ggcagcggtc tgggcggtgg tctcgacggg
      661 cttcttggcc atggtgctct ccatcctctg gtgcatgagc gataggtggt cccgtccgat
      721 ctcggcccgg gatgggatgg tttatacatg aacacttcgg cgatgcaaca tttattttgc
      781 actgcaccat cacgggatcg tgaacaccca ggcgtttggc gccgccccta ccccacagcg
      841 ctaggcagcc cgaaggcctg catctgggac cagaccttcg cgacatcatg gccgagcaca
      901 acgatgtcgt gcttcttgcc ggacttgtcg cgcacgtgat cacgcagcag cgcctcggcc
      961 ttgaagccga gcccctcgaa cagggcgatg ccgcccttct ggtccacggt catctgcacc
     1021 accagctttt cgagcccgga cgacagcgcc agcgagaatg cggcatgggt gagcacgcgc
     1081 cccaccccct cgccgcgcac gctgctcgac gtgacgaccc ggatttcgcc cacatggggc
     1141 gaccacgaca gcggatcggt gacgattgcc gcacagccca ccacctcctt gcccttcacg
     1201 gcgatgaggc tggtcatgcc gccgcgctcg ctctcggcga tccaggcctt cagcaccttc
     1261 gcctgggtaa tgtcgcgcgg caggaacagc agatcgtgca ccggcagcgc cttggcgaaa
     1321 gcggccacgg ccttttcgtc cttcgcgccc atcaggcgca actcgatctc gccgccctcg
     1381 atctgcgcct tgcgcggata ggtcttgatg tctccaaggc tcatacggaa cgtcccccca
     1441 gccagttgtc cagtttgggc cacaaacgct tgaccgcgtt ggggccggcg accacggaaa
     1501 tgtggccgcc cttgagcatc acttcttcct tgtcggtcga gccgattttc tcgatcagcg
     1561 gccgcgatgc gccatacggt atgatgtgat cgtgctcggc caccacatgc agaatcggcg
     1621 ccttgatgcg cgcgaggtcg gccttgcggc caccgaccgt catggtgccg ttgtacagcg
     1681 cattctccca catcagccgc ttggtggtct ggcggaaata ctcgccagca agcggcagcg
     1741 tatccgtcgc ccaccggtca aacatgcgat acgacttcac gtactcatcg ttccagatgt
     1801 tttcccaaag gtgcacctgg ctgacggcgc gcgaggccgg ccgcaacatc tcgaaggagg
     1861 tgaagatcag ttcgggcggc acgttgccgg cgctgtccac gagatggtcg acatcgaagt
     1921 gcttctgcga cgagaagttc tggaacagct tcatttcgcg gaagtcgact ggcgtcgtga
     1981 agcaaaccag gttcttcagc ggcccgcccg catgggttgc ggcgtagagc agagacagca
     2041 cgccgccgaa gcagtagccg cagatgttga cgtccttctc gcctgtatcc tcctgcacgc
     2101 atttcaccgc agtcggaatg aaatcgagca cgtaatcctc aaaggcgagg cgcttttcgt
     2161 ctggcttcgg cgcgttccag tccagcatga acacgtcgta cccccgcttc agcaggtatt
     2221 cgacgaagct ctgtcccggc gccatgtcca gaacgtagcc acggttggtc gtcgccatga
     2281 cgatcagcag cgggatgcgg taaatctcat cggtcagcgg acggtagtga tagaggttca
     2341 tcggaccgcg cgcatagatc acatccttcg gcgtgacgcc gaccggcggc gcggaggagg
     2401 cgaaatactc gacgcccttg attgagcgct ggatggcgcg ctgcacctcc tcctgcacgc
     2461 gggaggtgat gtctccagcc ggaccggacg cggccgaact cacgccttgc tcccagcggg
     2521 cggtggcttg cgcgtgcgcg gcggacgcgg cgcggccgtc ggcttcacca gcccaccaac
     2581 atcgggatgc gcccggtgca ggatggcgat gatctcgttg agccgtgcct cgaccgcctg
     2641 caaccgctcc ccgatattgg tcaactcctg ccggctcggc aggttcatgg tggcgagata
     2701 gcgctccatc atgtcgccca tggtcttctt cgcgcccatc gaggcgccgc ccatggtgtt
     2761 catgatcctc gagaaatcct ccgagcccat ggccttggtc gccatctcgt tgaatccctg
     2821 ctcgaactga ccgagcatct ggttgaacat cgccatggga tcgatgggtt tttccgacat
     2881 cggcgcgcct ccctgcgctg gctcccgccc attccaacgt gaaggtagcc cgaggtcaat
     2941 cgcgcaagca cttgcttccc ctgacgcacc ggcctaaaaa agttcatatc taaaccgata
     3001 gggatggcaa tggccacagt tgcacgaacg gacgagactt tcgccacggc caacggggtc
     3061 agcctgtgct ggcaggcttt cggcgaggaa agcgccgagc cgcttgtgct gatcatgggc
     3121 ctcggcgcgc agatggtgat gtgggaagat ccattctgtg aacagctcgc cgcgcgcggc
     3181 tttcgcgtca tccgcttcga caaccgcgac atcggccgct cgcagaagat gaccggcggc
     3241 gagcgcgtca ccttcgccga cctgattggc ctgtcgctgg ccggaaagcc gatccctgcg
     3301 gtttatacgc tgaaggacat ggcggctgat accgtcggcc tgcttgatgc gctcgggatt
     3361 gcccgtgccc acatcgtcgg cctctccatg ggcggcgcca tcgcccagga gatcgcgctc
     3421 ggctggccgg accgggtgaa gacactgacc tcgatcatgg cctcgccggg cggcgcaggc
     3481 ctgcccccgc cgacgccgga agccgccgcg gtgctcatga cgcccccgcc ggccacgaag
     3541 gaagaatatg tcgccacctt ccagcgcacc tggaaggtgc tgcgcgcggg ctccttcccg
     3601 caggacgagg cgactgatgc ggatcgcgcc gaacgcaact ggtcgcgcgg cctcaatcca
     3661 gccggcgtcg gtcggcagct cgtggcgatc ttcgc
//
LOCUS       DAOMXN010000233         3673 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_13802_length_3673_cov_8.747927, whole genome shotgun sequence.
ACCESSION   DAOMXN010000233 DAOMXN010000000
VERSION     DAOMXN010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13802_length_3673_cov_8.747927"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..609)
                     /locus_tag="PK812_12610"
     CDS             complement(127..609)
                     /locus_tag="PK812_12610"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRJ70435.1"
                     /translation="MGLIDGVTFGVFDRQQVADMTGREMLEAMMAGKLPAPTIGRALN
                     FILTEVGDGTAVFEGETNSGIMNPIGTVHGGWVLTMIDSATACAAQSTLPAGVSQTTV
                     ETKANMTRPIFVDTGRVRCEGRVISRGNQIITTEATVTDSAGRILAHGTSTVMVLRPR
                     "
     gene            complement(688..924)
                     /locus_tag="PK812_12615"
     CDS             complement(688..924)
                     /locus_tag="PK812_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70436.1"
                     /translation="MIVVRRLGFCMSLALLVTSAPVSMAHAAPAQGVVAADVSDYRRC
                     RRLVRRDMPGRKAGRHLQSLRIAAVQRCVMTGAF"
     gene            1166..2482
                     /gene="rimO"
                     /locus_tag="PK812_12620"
     CDS             1166..2482
                     /gene="rimO"
                     /locus_tag="PK812_12620"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HRJ70437.1"
                     /translation="MSEAPRISFVSLGCPKALVDSERIITRLRAEGYELTKTHAGADL
                     VVVNTCGFLDSAKAESLEAIGAALKENGKVIVTGCMGAEPEQIRDQFPNVLAITGPQA
                     YESVMGAVHAAAPPRHDPYLDLVPEQGVKLTPRHYAYLKISEGCNNRCSFCIIPKLRG
                     DLVSRPAGDVLREAEKLVKAGVKELLVISQDTSAYGVDLKYAESPWQDRVVRTRFFDL
                     AKELGSLGVWVRLHYVYPYPHVDEAIQLMADGVVLPYLDIPFQHAAPNVLKAMKRPAH
                     QEKTLERIRCWREICPDLAIRSTFIVGFPGETEEDFQLLLDWLSEAKLERVGAFKYEP
                     VRGAAANDLGLPMVPPEVQQQRYDRFMRHQQAISMRLMQRRVGKRLQVIVDEAGPSVA
                     RGRTKFDAPEIDGSVFIASRRPLRPGDVVTVKIERADAYDLHGTAV"
     gene            complement(2553..3266)
                     /locus_tag="PK812_12625"
     CDS             complement(2553..3266)
                     /locus_tag="PK812_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70438.1"
                     /translation="MLFRSSNAALFRGGTGDDPGAMMLEVELLELTHPLWATAFAALK
                     PSRWLPQSLMASINSLRHDRAILAQPDRRHLEQEIVPYLGRTGARDVLFVGTRSYTTH
                     YPALFEQAGMTLWTTDIDPFAARYGVAGRHVTIDATHLTPETFGRRFDAVVFSGVIGF
                     GLDSDVQISRAASALSQLITNDGVLVLGWNSDRSADPLDNPVWRRLFRRSTRAGAVGR
                     TSFRTCTHVFDVLEADPEA"
     gene            complement(3283..>3673)
                     /locus_tag="PK812_12630"
     CDS             complement(3283..>3673)
                     /locus_tag="PK812_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70439.1"
                     /translation="AQPRPAGGARQQAGIPIAEAVKMIGEKHDLTNLIWLRQRGGNQL
                     VRLWEGGEARVYALTKDGLRPTNRNFARLFHEGNFAGIWSGLMNVIISFALLLLTVTG
                     AWQFVTKQIRKYRNRRLKAEWGKAQAA"
BASE COUNT          641 a         1201 c         1207 g          624 t
ORIGIN      
        1 ggtccagtcc agcagcgagc gtcggatcgt cgcgcgcgca cccggcgcta tggcggcaga
       61 cggcgtgccg acggcgaaat ccgccagcgc gcccgtgatg gaaggagggc ccgccgccgg
      121 catggctcag cgtggcctca gcaccatgac ggtcgaggtg ccgtgcgcga gaatcctgcc
      181 ggcgctgtcc gtcacggtcg cttccgtcgt gatgatctgg ttgccgcggc tgatgacgcg
      241 cccctcgcag cgcacccggc ctgtgtcgac gaagatcggt cgcgtcatgt tcgccttggt
      301 ctcgaccgtg gtctggctga caccggccgg caacgtcgac tgcgcggcgc aggccgtggc
      361 gctgtcgatc atcgtcagca cccagccgcc atgaacggtg ccgatcgggt tcatgatgcc
      421 ggaattcgtc tcgccctcga acaccgccgt gccgtcgcca acctcggtca ggatgaagtt
      481 gagcgcccgt ccgatggtcg gcgccggcag ctttcccgcc atcatggctt cgagcatctc
      541 gcggcctgtc atgtccgcga cctgctggcg gtcaaacacg ccaaacgtca cgccatcgat
      601 aagtcccatg ccatcccccg gttggttcat gcatgaactt atccggcggg accgaattgg
      661 gaagcggaaa tgcgtgatcc cgcgcgctca gaacgcgccc gtcatgacgc agcgttgcac
      721 ggcggcgatg cgcaggctct ggagatgcct tcccgccttc cggcccggca tgtcgcgccg
      781 caccagccgt cggcagcggc gataatcgct gacgtccgca gcgacgaccc cctgggccgg
      841 cgcggcatgc gccatgctga ccggcgcgct ggtcacaagc aacgcgaggg acatgcaaaa
      901 gccaaggcgg cgcacgacga tcatcgccct ctcccatcat cgcgccgccc gcgacttatg
      961 ttgggcaggc tggatgaacc ggcgctgaat gcgtgaaggt tgacagaacc gccccgaaat
     1021 gcaccagacc ggcgctctga caccatcggt cacgagacgt gaccgatgac cacttctgaa
     1081 ttttcacttc ttacaatggg aatgaaaatt cagaacaggc gccaagggtc attggtcgtg
     1141 acccttggta tgaccggagc ctttcatgag cgaagccccc cgcatttcct tcgtgtccct
     1201 cggctgcccc aaggcgctgg tggacagcga gcgcatcatc acgcgcctgc gcgccgaggg
     1261 ctatgaactg accaagacgc atgccggcgc ggacctcgtg gtcgtcaaca cctgcggttt
     1321 cctcgacagc gccaaggcgg aatcgctcga agccatcggc gccgcgctca aggaaaacgg
     1381 caaggtcatc gtcacgggct gcatgggcgc ggaacccgag cagatccgcg accagttccc
     1441 gaacgtgctg gccatcactg gcccgcaggc ctacgagagt gtgatgggcg cggtccatgc
     1501 cgccgcgccg ccaagacacg atccctacct cgatctggtg ccggaacagg gcgtcaagct
     1561 cacgccgcgt cactacgcct acctgaagat ttccgagggc tgcaacaacc gctgttcctt
     1621 ctgcatcatc ccgaagctgc gcggcgatct cgtctcgcgt ccggccggcg acgtgctgcg
     1681 cgaggctgaa aagctcgtga aggccggcgt gaaggaactg ctggtcattt cgcaggacac
     1741 gtccgcctac ggcgtcgatc tgaaatatgc cgaaagcccc tggcaggatc gcgtcgtgcg
     1801 cacccggttc ttcgatctgg cgaaggagct cggatcgctc ggggtctggg tgcggctgca
     1861 ctacgtctac ccctatccgc atgtggacga ggccatccag ctcatggccg acggcgtggt
     1921 gctgccgtac ctcgacattc cgttccagca cgccgcaccg aatgtgctga aggcgatgaa
     1981 acgaccggcc caccaggaga agacgctgga gcgaatccgc tgctggcgcg agatctgccc
     2041 ggacctggcc atccgctcga cctttatcgt cggctttccc ggcgagaccg aggaggattt
     2101 ccagctgttg ctggactggc tgtccgaagc gaagctggag cgtgtcggcg ctttcaagta
     2161 tgagcctgtg cgcggcgccg cggcgaacga tctcggcttg ccgatggtcc cgcccgaggt
     2221 ccagcagcag cgttacgacc gcttcatgcg ccaccagcag gcgatttcca tgcggctgat
     2281 gcagcggcgc gtcggcaagc ggcttcaggt gatcgtggat gaggccggcc cgagcgttgc
     2341 ccgcggacgc acgaaattcg acgcgccgga gatcgacgga tcggtgttca tcgcctcgcg
     2401 gcggccattg cggccgggcg acgtcgtcac ggtcaagatc gagcgcgccg acgcctatga
     2461 cctgcacggg acggcggttt gagcggtgtt tcaggcgccg ggagtcaggc cttgcggccc
     2521 gtggagcgga tcgtgaggtt ggcgtaaggc catcaggctt ccgggtccgc ttccagcacg
     2581 tcgaagacgt gggtgcaagt ccggaagctc gtgcgcccga cggcgccggc ccgcgtgctg
     2641 cgtcggaaca ggcgacgcca gaccggattg tcgagcggat cggccgaacg gtccgaattc
     2701 cagcccagaa ccagcacgcc atcgttcgtg atcagttggg acagcgcgga ggctgcccgg
     2761 ctgatctgca cgtccgaatc caggccgaag ccgatgactc cgctgaacac aacggcatcg
     2821 aacctgcgcc cgaacgtctc gggggtcaga tgcgtcgcat cgatggtgac atgacggcca
     2881 gcaacgccat agcgcgctgc aaagggatcg atgtcggtgg tccacagcgt catgccggcc
     2941 tgctcaaaca gggccggata gtgcgtggtg taggagcggg tgccaacgaa cagcacgtcc
     3001 cgcgcgccgg tccggcccag gtaagggacg atctcctgct ccagatggcg ccggtcgggt
     3061 tgggcaagaa tggcccggtc gtggcgaagg gaattgatcg aggccatcag cgattgtggc
     3121 agccagcgcg agggcttgag agccgcgaac gctgtcgccc acagcggatg tgtcaattct
     3181 agcagttcaa cttccagcat catcgccccg ggatcgtcgc ctgtgccacc ccggaacagg
     3241 gcggcgttgg acgaacgaaa cagcaagcgc cgtgccagtc gttcaggcgg cctgtgcctt
     3301 gccccactcc gccttcaggc gtcggttcct gtacttgcgg atctgcttgg tgacgaactg
     3361 ccaggcgccg gtcacggtca gcagcagcag cgcgaaggag atgatgacgt tcatcaggcc
     3421 tgaccagatg ccggcgaaat tgccttcgtg gaacaggcgg gcgaagttgc ggttcgtcgg
     3481 ccgcagcccg tccttggtga gggcatagac gcgcgcttcc ccgccttccc agaggcggac
     3541 aagctggttt ccgccgcgct ggcgcagcca gatcaggttg gtcaggtcgt gcttctcgcc
     3601 gatcatcttc accgcctcgg cgatcgggat gccggcttgc tggcgggcgc cgccggcagg
     3661 tcgcggttgc gcc
//
LOCUS       DAOMXN010000234         3655 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_13913_length_3655_cov_10.408889, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000234 DAOMXN010000000
VERSION     DAOMXN010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13913_length_3655_cov_10.408889"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..410)
                     /locus_tag="PK812_12635"
     CDS             complement(111..410)
                     /locus_tag="PK812_12635"
                     /inference="COORDINATES: protein motif:HMM:NF021255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2384 domain-containing protein"
                     /protein_id="HRJ70440.1"
                     /translation="MLPLWVDRLLGRPPPPPLSDPAARVQFFLNRMRPAARARAEAVR
                     REAIEDFGSEQAADDWLCASIPFLGDRRPIEIIHTARGAGEAHTCLNNMRWGIYS"
     gene            complement(416..1402)
                     /locus_tag="PK812_12640"
     CDS             complement(416..1402)
                     /locus_tag="PK812_12640"
                     /inference="COORDINATES: protein motif:HMM:NF025772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4424 family protein"
                     /protein_id="HRJ70441.1"
                     /translation="MNALRTLWSTITVVALVAAVPAARANDSMAATAIGGLTLVKSDA
                     IRMDSEDLYISEKQVRVAYRFTNTTDRDIDTLVAFPLPDYVAAPTQVVLDFANDIRFE
                     TRIDGKPAKLDFVQQAIFKGQDITARLTALGVPPVAQYHIFEAALKALPKSEVDRLVQ
                     DGLLQNIGAPDQPDWLAKWLVRTTVTRTQVFPAGKTVSVEHSYNPLPGGSVGGGLDRR
                     YRKEPDFAAKKKAYCIDDAFIRGFEARQKTLREPNAYSEVWLAYVLKTGANWAGPIRD
                     FRLVVDKGDPDALVSFCAAGVKKTGPTTFEVRAKDFTPKQDLNVLIVKFEKP"
     gene            1475..1672
                     /locus_tag="PK812_12645"
     CDS             1475..1672
                     /locus_tag="PK812_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70442.1"
                     /translation="MTRFDWLKKHEVHLERGDVVKLQGDFAYCRARRDLARRSSLRRA
                     WDDRVRVIERRLRDVEAAKEE"
     gene            1679..2683
                     /gene="lipA"
                     /locus_tag="PK812_12650"
     CDS             1679..2683
                     /gene="lipA"
                     /locus_tag="PK812_12650"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HRJ70443.1"
                     /translation="MPATIDLLNNDPRKKSGGDASAGENSRALRHPEKAHRPDNPIQR
                     KPEWIRVKAPGSPKYAETRSIVKENKLVTVCEEAGCPNIGECWEKKHATFMIMGDTCT
                     RACAFCNVKTGLPEPLDANEPAYIAEAVAKLGLEHVVITSVDRDDLADGGAEHFAKVI
                     RAIRAGSPKTTIEILTPDFLRKDGALEVVVAARPDVFNHNLETVPSKYLKVRPGARYF
                     HSIRLLQRVKELAPSIFTKSGIMVGLGEERNEVLQLMDDLRSADVDFITIGQYLQPTK
                     KHHAVLRFVPPDEFKGYETVAYAKGFLMVSATPLTRSSHHAGEDFARLRAARAALKGA
                     "
     gene            2984..>3655
                     /locus_tag="PK812_12655"
     CDS             2984..>3655
                     /locus_tag="PK812_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HRJ70444.1"
                     /translation="MRAAVAASLGLLLANCASQAPVERRSHSKEVGAFPQAKYGAASP
                     RVVSVDQDAPKGGGRYHVGNPYRVAGKTYVPYERPVGHTSSGLASWYGEAFHGRKTAN
                     GEVYDRRSITAAHPTMPLPSYARVTNMRNGHSIIVRVNDRGPFHGGRVMDVSERVANA
                     LNFKHQGTTRVKIDYLGKAGLTGSDDNRLLASLRTDGQPANSPGLGTATTMVASAGPA
                     AQPNAT"
BASE COUNT          661 a         1139 c         1220 g          635 t
ORIGIN      
        1 tggagacgac cgaggaagac ctgatgcaca ccgtcttccc gcaccccacg ctctccgaga
       61 tgatgaagga aagcgtactg gatgcctatg gcagggcgct gaatgcgtag tcacgaataa
      121 atcccccagc gcatgttgtt gaggcaggtg tgcgcctcac cggcgccgcg cgccgtgtgg
      181 atgatctcga tcggccgcct gtccccgagg aaggggattg aggcgcagag ccagtcatcc
      241 gcagcctgtt ccgagccgaa atcctcgatg gcttcgcggc gcaccgcctc ggcgcgggcg
      301 cgcgccgccg gcctcatgcg gttcaggaag aactgcacac gcgcggccgg gtccgagagc
      361 ggaggcggtg gcgggcggcc gagcaggcga tcgacccaga gcggcagcat ggcgcctagg
      421 gcttctcgaa cttcacgatc aggacattca ggtcctgctt tggtgtgaaa tccttggcgc
      481 gcacctcgaa cgtcgtcggg ccggtcttct tcacgccggc ggcgcagaag ctgaccagtg
      541 catccggatc gcccttgtcc accacaaggc ggaaatcgcg gatcgggccg gcccagttcg
      601 cgccggtttt tagcacgtag gcgagccaga cctcgctgta ggcgttgggt tctcgaaggg
      661 ttttctgccg cgcctcgaag ccccggatga aggcgtcgtc gatgcagtac gccttcttct
      721 tggctgcgaa gtccggctcc ttgcggtagc gccggtcaag cccgccgcca acgcttccgc
      781 cgggcagagg attgtaactg tgctcgacgc tgacggtctt gccggccgga aagacctgcg
      841 ttcgggtcac ggtggtgcgc accagccatt tcgccagcca gtccggctgg tccggcgcgc
      901 cgatgttctg cagcaacccg tcctgcacca gccggtcgac ctccgacttc ggcagggctt
      961 tcagcgcggc ttcgaaaatg tggtactggg caaccggcgg cacgccgaga gccgtcagcc
     1021 gggcggtgat gtcctggccc ttgaagatgg cctgctgcac gaagtcgagt tttgccggct
     1081 tgccgtcgat ccgcgtctcg aacctgatgt cgttggcgaa gtcgagcacg acctgcgtcg
     1141 gggccgcgac atagtcgggc agggggaaag cgacgagcgt gtcgatgtcc ctgtccgtgg
     1201 tgttggtgaa gcgataggcg acgcgcacct gcttttccga aatgtacagg tcttccgaat
     1261 ccatgcggat cgcgtcggac ttgaccagcg tgaggccccc gatggcggtg gctgccatgc
     1321 tgtcgttggc gcgggcggcc ggcacggcag cgaccagggc cacaaccgtg atggtgctcc
     1381 agagtgtgcg cagagcgttc atgcagaaat ggtccttcac acccgcgcgg gacgtgctac
     1441 gtcatgcggc gatccaatgc tccaagagct gatcatgacc cgtttcgact ggttgaaaaa
     1501 gcacgaagtt catctggagc ggggcgatgt cgtcaagctt caaggggatt tcgcctattg
     1561 tagggccagg cgcgatctgg cgcggcggtc aagcttgcgc agggcatggg acgaccgcgt
     1621 gcgcgtgatc gagcgccgcc ttcgcgacgt tgaggcggcc aaggaggagt aacgccgcat
     1681 gccggccacc atcgaccttc tgaacaacga tcctcggaag aagtcaggcg gggacgcctc
     1741 ggcaggtgag aactcccgtg cgcttcgcca tccggagaag gcgcaccggc cggacaaccc
     1801 aatccagcgc aagccggagt ggattcgcgt caaggcgccg ggctcgccga aatatgccga
     1861 gacccgcagc atcgtgaagg agaacaagct cgtcacggtg tgcgaggagg caggttgtcc
     1921 caatatcgga gaatgttggg agaaaaagca cgccaccttc atgatcatgg gcgacacctg
     1981 cacgcgggcc tgcgcgttct gcaacgtgaa gaccgggttg ccggagccgc tcgatgcgaa
     2041 cgagccggcg tatatcgccg aggccgtggc caagctcgga cttgagcatg tggtcatcac
     2101 ctcggttgac cgggacgatc ttgcggacgg cggcgccgag catttcgcca aagtcatccg
     2161 ggcgatccgc gcgggcagtc cgaagacgac catcgagatc ctgaccccgg acttcctgcg
     2221 caaggatggc gcgctggaag tcgtcgtcgc cgcgcggccg gatgtgttca accacaatct
     2281 cgaaaccgtg ccgtcgaagt atctcaaggt gcggcccggc gcgcgctact tccattccat
     2341 ccgcctgttg cagcgggtga aggagctggc cccgtcgatc ttcaccaagt ccggcatcat
     2401 ggttgggctc ggcgaggagc ggaacgaggt gctgcaattg atggacgatc tgcgctcggc
     2461 cgacgtcgat ttcatcacca tcggccagta tctccagccc acgaagaaac atcatgcggt
     2521 cctgcgcttc gtgccgccgg atgaattcaa ggggtatgaa accgtcgcct acgccaaggg
     2581 gttcctgatg gtgtcggcaa cgccgctgac gcgctcgtcg catcatgctg gcgaggattt
     2641 cgctcgcctt cgggccgccc gcgcggcgct gaaaggcgca tgaggcgacc tgtccgccgg
     2701 gggtggccat tgtgtcgcag gggcggcatt tcgcatggcc agcccaaatc ccgccgcgat
     2761 tcagtcgcat tcggcgggcg ttcatcgtgt tgcgaacggg cgcttgaccg gttggcgtag
     2821 gctctttaac cggcaggcaa cctcaacgga ctaagatccg aaacgataaa atcgcgcgga
     2881 aggcgtatcg atccgcgtgg ccggagttgg aatggctgaa gattttcggg gcgaaccgtg
     2941 tcgtcatgac gcggtgagga acaggtctgc atcccgcagc gccatgcgcg cggctgttgc
     3001 ggcgtcgctt ggtttgctgc tggcgaactg cgccagtcag gcaccggtcg agcggcgcag
     3061 ccacagcaag gaagtcggcg cgtttcccca ggccaaatac ggcgcggcca gtccccgcgt
     3121 cgtcagcgtc gatcaggacg cgccaaaggg gggcggacgc taccatgtcg gcaatcccta
     3181 tcgcgtcgcc ggcaagacct atgtgccgta tgaaaggccg gttggccata cgagttccgg
     3241 cctggcctcc tggtatggcg aggcattcca cggtcgaaag accgccaacg gcgaagtgta
     3301 tgatcgccgc tcaatcaccg cagcgcatcc gaccatgccg ctgccgagct atgcgcgcgt
     3361 gaccaacatg cgcaacggcc attccatcat cgtgcgggtc aatgaccgcg gtccgttcca
     3421 cggcgggcgc gtgatggacg tgtccgagcg ggtggccaac gcactgaact tcaagcatca
     3481 gggcaccacc cgggtgaaga tcgactacct cggcaaggcg ggcctgacgg gcagcgacga
     3541 caatcgcctg ctggcctcgt tgcgcacgga cgggcagccg gccaattcgc ccggcctcgg
     3601 cacggcgacc accatggtgg cgtcggccgg tcctgcggcc cagcccaatg cgacc
//
LOCUS       DAOMXN010000235         3594 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_14244_length_3594_cov_11.256287, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000235 DAOMXN010000000
VERSION     DAOMXN010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14244_length_3594_cov_11.256287"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..1572)
                     /gene="purF"
                     /locus_tag="PK812_12660"
     CDS             complement(82..1572)
                     /gene="purF"
                     /locus_tag="PK812_12660"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014130186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRJ70445.1"
                     /translation="MSGLHETFGAGYPVEGVELGRESDDLREKCGVFGVYGHPDAAAI
                     TALGLHALQHRGQEAAGIVSFDGKRFHSERRLGLVGDHFSSAETINRLPGTAAIGHVR
                     YSTTGETILRNVQPLFAELHGGGFALAHNGNLTNGLTLRRELVQSGALYQSTSDTEAL
                     LHLVARSRRNRFLDRFIDGLNQIEGAYAFVAMTNKKLIGARDPLGFRPLVLGLLDGCY
                     ILASESCALDIIGARYVRDVEAGEIVVITDDGLESHKPFPPQQTRPCVFEYIYFARPD
                     SLVHGRPVYDVRKKLGAELARESGVPADVVIPVPDSGVPAAIGYAQGSGIPYELGIIR
                     NHYVGRTFIQPTQSTREVGVRMKHSANRVVVKGKRIILIDDSIVRGTTSVKIVKMMRD
                     AGAKEVHFRIASPPITHPDYYGIDTPDREKLMAATHNLEEMRRYIGCDTLAFLSIDGL
                     YRALGFESRGPRPAFADHCFTGDYPTHIRDLVGEGPKQLSMLAEGA"
     gene            complement(1619..2224)
                     /locus_tag="PK812_12665"
     CDS             complement(1619..2224)
                     /locus_tag="PK812_12665"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HRJ70446.1"
                     /translation="MPVSILDMIVIGILLVSGFLAMLRGFTREVLAIASWVVAGLCAY
                     LFYKQLTPYVMQNIIANEKIAQPAAAGAIFLGALIVAYFVTSKLSDVILDSRIGALDR
                     TLGFIFGVARGLLLAVVAFGLFEKLVGEKQQPTWVADAKLRPMLKSSADRLMGLLPED
                     LEQQVMSKIKKEAPGGEQPKEPPTTPGVPPRPGQPAQPPAR"
     gene            complement(2409..>3594)
                     /gene="radA"
                     /locus_tag="PK812_12670"
     CDS             complement(2409..>3594)
                     /gene="radA"
                     /locus_tag="PK812_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012456116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HRJ70447.1"
                     /translation="AGIAELDRVTGGGFVRGSVILLGGEPGIGKSTLLIQAAAAIALG
                     GHRSVYISGEEATAQVRLRAQRLGLSASPVELASETSVEDILATLALGAPARLVIIDS
                     IQTMWTEAVEAAPGTVTQVRASAQALIRYAKSSGAAVILVGHVTKDGQIAGPRVVEHM
                     VDAVMSFEGDSGHQFRLLRAVKNRYGATDEIGVFEMTGGGLAEVANPSALFLAGRDPQ
                     QAGTAVFAGMEGTRPLLCEIQALVTPSPPGTPRRAVVGWESSRLAMVLAVLEAHGGIR
                     LGQHDVYLNVAGGLRIGEPAADLAVAAALLSSLSGTPLPQEAVFFGEISLSGAIRPVA
                     HAPLRLKEAAKLGFRRALLAEAGSDQIERHGMDATTLRRIADLVGEIAVGQLEDRRAR
                     SP"
BASE COUNT          638 a         1133 c         1231 g          592 t
ORIGIN      
        1 caggccgagg cagatggcgc gaccgatgcc gcgcgaggca cccgtgacga gtgcgatacg
       61 tccttccagc gccttcatgg ttcaggcccc ttccgccagc atggagagct gcttgggccc
      121 ttcgccgacg agatcgcgga tgtgggtggg atagtcgccg gtgaaacaat gatcggcgaa
      181 tgcgggcctt ggtccacggc tttcgaagcc gagcgcacgg tacagcccgt cgatggagag
      241 gaaggcaagg gtatcgcagc cgatgtagcg gcgcatttcc tcgaggttat gcgtcgccgc
      301 cataagcttt tcgcggtccg gcgtatcgat gccgtagtag tcggggtgcg tgatcggcgg
      361 cgaggcgatg cggaaatgta cttccttcgc tccggcgtcg cgcatcatct tgacgatttt
      421 taccgatgtg gtgccgcgca cgatggaatc gtcgatcagg atgatgcgct tgcctttgac
      481 gacgacgcgg ttggcggaat gcttcatgcg cacgccgact tcgcgcgtcg attgcgtcgg
      541 ctggatgaag gtgcgcccga cgtagtgatt gcggatgatg ccgagttcgt agggaatgcc
      601 ggagccttgg gcatagccga tggcggcggg aacgccggaa tccggcaccg gaatgacgac
      661 atcggcgggg acgcccgatt cccgcgccag ttccgcgcca agcttctttc gcacgtcgta
      721 gaccgggcgg ccatgcacca acgagtcggg acgggcgaag tagatgtatt cgaacacgca
      781 ggggcgcgtc tgctgcggcg ggaacggctt gtgtgactcc agcccgtcgt cggtgatgac
      841 gacgatctcg ccagcctcga catcgcgtac gtaacgggcg ccgatgatgt ccagcgcgca
      901 ggattcggac gccaggatgt agcacccgtc caggaggccc agcaccagcg gcctgaagcc
      961 gagtggatcg cgcgcgccga tcagtttctt gttggtcatc gccacgaagg cataggcgcc
     1021 ttcgatctgg ttcagcccgt cgatgaagcg gtcgaggaaa cggttgcggc gcgaacgggc
     1081 gacgagatgc agcagcgctt cggtgtcgga agtcgactga tagagcgcgc cggactgcac
     1141 gagttcacgc cgcagggtca ggccgttggt caggttaccg ttgtgcgcca gcgcgaaacc
     1201 gccgccgtgc agctcggcga acagcggctg cacgttacgc aggatcgtct cgccggtcgt
     1261 ggaatagcgc acatggccga tggctgcggt tccaggcagg cggttgatcg tctcggcgga
     1321 cgagaaatga tcccccacca ggcctagacg gcgttccgaa tggaagcgct tgccatcgaa
     1381 ggagacgatg ccggccgctt cctgcccgcg atgctggagt gcgtgcaggc cgagcgccgt
     1441 gatcgcggcg gcatcggggt ggccatagac gccaaagacg ccgcacttct cgcgcaggtc
     1501 gtcggattcg cggcccagtt cgacaccttc cacggggtaa cccgccccga aggtttcatg
     1561 cagtccagac atcgatcatt cccgtagcgg gcggcggcgc cgccggcagg tgaagtcctt
     1621 atcgcgcagg cggctgcgcc ggctgtcccg gacgtggtgg aacgccaggg gtggtcggcg
     1681 gttccttggg ctgttctcct ccgggagctt ctttcttgat cttggacatg acctgctgct
     1741 caaggtcttc cggcagcagg cccatcaggc ggtcggcgga actcttcagc atgggacgca
     1801 gcttggcatc cgcaacccag gtcggctgtt gcttttcccc aaccagtttc tcgaaaagac
     1861 cgaaggccac gactgccagc aacaggccgc gcgcaacgcc gaagatgaag ccgagcgtgc
     1921 gatcaagcgc gccgatgcgc gagtcgagga tcacgtccga gagcttggag gtgacgaaat
     1981 acgccacgat cagcgcgccg aggaagatgg cgccggcggc agcaggctgg gcgatcttct
     2041 cgttggcgat gatgttctgc atcacgtagg gcgtcagctg cttgtagaac agataggcgc
     2101 acagaccggc gacaacccag gacgcgatgg ccagaacctc gcgcgtgaat ccgcgcagca
     2161 tggccagaaa gcccgacacc agcagaatgc cgatgacgat catgtccaga atggaaacgg
     2221 gcattgttgt cttccccggt gcgcgcaaca ggatcgggcc ctccggagga ggtggaagcg
     2281 gcctgatccc caactgatcg aagaactccg ctcgcgcgga gccatgccgc tctataattg
     2341 cagtgcgtca gttagtcacg agttagtcac gacgccgcgt aaagaattca catcgcgtgc
     2401 cggcggcctc atgggctgcg tgcgcgccga tcctcgagct ggccgacggc gatctcaccg
     2461 accagatccg cgatgcggcg cagcgtggtc gcgtccatgc cgtggcgctc gatctggtcg
     2521 ctgcccgcct cggccagcag cgcccgcctg aatccgagct tggccgcttc cttcagacgg
     2581 agcggggcat gggcgaccgg ccggatggcg ccggaaaggc tgatttcgcc gaaaaaaacc
     2641 gcttcctgtg gcagcggcgt tccggacagc gacgacagca aagccgccgc gacagcgaga
     2701 tcggccgccg gctcgccaat gcgcaggccg ccggcgacgt tcagatagac atcatgctgg
     2761 ccgaggcgga tgccgccatg tgcttccaga acggccagca ccatggcaag gcgggaggac
     2821 tcccagccga cgacggcgcg gcgcggcgtg ccgggcggtg acggcgtgac cagcgcctgg
     2881 atttcgcaga gcaggggccg cgtgccttcc atgccggcga aaacagccgt gccggcctgc
     2941 tgcggatcgc ggccggcgag gaacagggcg gaagggttgg cgacttccgc caggcctccg
     3001 ccggtcattt cgaacacgcc gatctcgtcc gtggcgccgt agcggttctt gaccgcacgc
     3061 agcaggcgga actggtggcc ggaatcgccc tcgaaggaca tcacggcgtc gaccatgtgc
     3121 tcgaccacgc gcggtcccgc gatctgcccg tccttggtca cgtggccgac gagaatcacc
     3181 gccgcgccgc tggacttggc gtagcggatc aacgcctgcg ccgaggcacg cacctgcgtc
     3241 accgtgccgg gcgcggcctc cacggcttcg gtccacatcg tctggatcga atcgatgatg
     3301 acgagccgcg ccggcgcgcc gagcgccagc gtcgccagga tatcctcgac cgaggtctcg
     3361 gaggccagtt ccacggggga agcgctcaag ccaagccttt gagcgcgcag gcgaacttgc
     3421 gccgtggctt cctcgcccga gatgtagacg ctgcgatggc cgccgagcgc gatcgccgcc
     3481 gccgcctgaa tgagcagggt cgacttgccg atgcccggtt cgccgccgag caggatcacc
     3541 gagccgcgca cgaagccgcc gcctgtgacg cggtcgagtt cggcgatgcc ggcc
//
LOCUS       DAOMXN010000236         3574 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_14350_length_3574_cov_12.591077, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000236 DAOMXN010000000
VERSION     DAOMXN010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14350_length_3574_cov_12.591077"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PK812_12675"
     CDS             <1..199
                     /locus_tag="PK812_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015823336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70448.1"
                     /translation="SAAVRAEDFTAAMAAMARLRGPVDAFFDKVLVNAADPVLRLNRL
                     RLLSDLRRATHNVADFSKVGG"
     gene            367..3045
                     /gene="ppdK"
                     /locus_tag="PK812_12680"
     CDS             367..3045
                     /gene="ppdK"
                     /locus_tag="PK812_12680"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HRJ70449.1"
                     /translation="MTKWVYTFGDGRAEGESSLRNLLGGKGANLAEMSNLGLPVPPGF
                     TISTEVCTYFYENGKTYPADLAAQVDAALQHIGALSGRTFGDAANPLLVSVRSGARAS
                     MPGMMDTVLNLGLNDVTVVALAQSANDERFAWDSYRRFITMYADVVLEVDHAHFEDVL
                     DEYKDMKGYASDTDVSASDWKHLVGEYKAVVERELGRSFPQDPREQLWGAIGAVFGSW
                     MNPRANKYRELHGIPASWGTAVNVQAMVFGNMGDTSATGVAFTRNPSTGAKELYGEFL
                     INAQGEDVVAGIRTPQDITEAARIAAGSAKASMEAAMPVAYGELVRIYGLLERHYRDM
                     QDMEFTVEKGKLWMLQTRNGKRTAKAALRIAVDLANEGLISREEAVLRVDPASLDQLL
                     HPTIDPKAERKIIGSGLPASPGAAAGEIVFNSEDAEQAVKDKRKVILVRVETSPEDIH
                     GMHAAEGILTTRGGMTSHAAVVARGMGKPCVSGAGTLRVDYAKGVLLAGGHQLKKGDF
                     ITIDGSTGQVLLGRVKMLEPELSGEFANLMEWADAGRRLKVRANAETPADARQARSFG
                     AEGIGLCRTEHMFFDEDRIISMREMILAGDEKGRRAALAKLLPMQRADFEQLFEIMSG
                     LPVTIRLLDPPLHEFLPHTAEEIADVARAMGADPERLRQRAAELSESNPMLGFRGCRL
                     AVAYPEIAEMQARAIFEAAVAAAKKTGKPVTPEVMVPLVMTKPELDHVKARIDAMAVA
                     VKRETGAEFAYQVGTMIELPRAALRAEEIARSAEFFSFGTNDLTQTTLGISRDDAAHF
                     LEPYKRRGILAEDPFMTLDQDGVGELVKIAAERGRKTRPDIKLGICGEHGGDPASIGF
                     CEMTGLDYVSCSPFRVPVARLAAAQAALGKVTKAEG"
     gene            3273..>3574
                     /locus_tag="PK812_12685"
     CDS             3273..>3574
                     /locus_tag="PK812_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70450.1"
                     /translation="MHRPRSQAFRPKKTARWVAKALAPWSVASCLLISFTASAGPRID
                     AFDARRASIIDLASVTLPPRIAPGRETGPVVPGYSGLREAPLADVVRWLVPPSVGGL"
BASE COUNT          614 a         1211 c         1189 g          560 t
ORIGIN      
        1 cagcgcagcc gtccgggcgg aggatttcac ggcggcgatg gccgccatgg cgcgtctgcg
       61 cggccccgtg gatgcatttt tcgacaaggt cctggtcaac gcggccgatc cggtgctgcg
      121 cctgaaccgc ctgcgcctgc tctccgacct caggcgcgcc acccataacg tggcggactt
      181 ctccaaggtg ggcggctgat cttctcccgg cgcatggggg cgctgcaaaa tgccgctggc
      241 ggatcggcct gcggcggatt aaccatgcgc cctccggcat ttgcatttct gctaatgtgt
      301 tcatgaagcc gaaaggcggc agcgcctatt gcactgcaca cggacggtca gaaacggggt
      361 cgcacgatga cgaagtgggt ctatacgttt ggtgacggca gggccgaggg cgaatcatcc
      421 ctgcgcaacc tgctgggcgg caagggtgcg aaccttgcgg aaatgtcgaa ccttggcctg
      481 ccagtgccgc cgggcttcac catatccacg gaggtctgca cctactttta cgagaacggc
      541 aagacctatc ccgccgatct cgccgcccag gtcgatgcgg cattgcagca catcggcgcc
      601 ctgagcgggc gcaccttcgg cgatgcggcc aacccgttgc tcgtttccgt gcgctccggc
      661 gcgcgcgcct cgatgcccgg catgatggat accgtcctca atctcggcct caatgatgtg
      721 acggtcgtgg cgctggcgca atcggccaat gacgagcgct tcgcctggga cagctaccgg
      781 cgcttcatca ccatgtatgc cgacgtggtg ctggaagtcg atcacgccca tttcgaggac
      841 gtgctggacg aatacaagga catgaagggc tacgcctccg acacggatgt gagcgcgtcg
      901 gactggaagc atctggtcgg cgagtacaag gccgtggtgg aacgcgaact cggacggtcg
      961 ttcccgcagg atcccagaga acagctgtgg ggcgccatcg gtgccgtttt tggttcctgg
     1021 atgaacccgc gcgccaacaa gtatcgcgag ctgcacggca tccccgccag ctggggcacg
     1081 gccgtcaacg tgcaggccat ggtgttcgga aacatggggg acacgtccgc gacgggcgtc
     1141 gccttcacgc gcaatccatc cacgggcgcg aaggagctct atggcgagtt cctgatcaac
     1201 gcacagggcg aggacgtggt ggccggcatc cgcacgccgc aggacatcac ggaagcggcc
     1261 cgcatcgcgg ccggatccgc caaggcatcg atggaagccg ccatgccggt ggcctacggc
     1321 gagctcgtcc gcatctacgg cctgctcgaa cggcactacc gtgacatgca ggacatggaa
     1381 ttcacggtcg agaagggcaa gctctggatg ctccagacgc ggaacggcaa gcgcacggcc
     1441 aaggcggcgc tgcgcatcgc cgtcgatctc gccaacgaag gcctgatcag ccgcgaggaa
     1501 gccgtgctgc gcgtcgatcc ggcctcgctc gaccaactgc tgcatccgac catcgacccg
     1561 aaggccgaac gcaagatcat cggctccggc ctgcctgcct ctcccggcgc cgcggcaggc
     1621 gagatcgtct tcaattccga ggacgccgag caggccgtga aggacaagcg caaggtcatc
     1681 ctcgtgcgcg tcgaaacctc gccggaggac atccacggca tgcatgccgc cgaaggcatc
     1741 ctgacaacgc gcggcggcat gaccagccac gccgccgtcg tggcgcgcgg catgggcaag
     1801 ccctgtgttt ctggcgccgg caccctcagg gtggactacg ccaagggcgt gctgctcgcc
     1861 ggcggccacc agctcaagaa gggcgacttc atcaccattg acggctccac cgggcaggtg
     1921 ctgctcggcc gggtgaagat gctggaaccg gaactgtccg gcgagttcgc caacctgatg
     1981 gaatgggccg acgccggccg gcggctcaag gtgcgggcca acgccgagac ccctgccgat
     2041 gcgcgccagg cccgctcctt cggcgcggaa ggcatcggcc tgtgccgcac cgagcacatg
     2101 ttcttcgacg aggaccgcat catctccatg cgggaaatga tcctcgccgg cgacgagaag
     2161 ggtcgccgcg ccgcgctcgc caagctgctg cccatgcagc gcgccgactt cgagcaactg
     2221 ttcgagatca tgtccggcct gccggtgacg atccgcctgc tcgatccgcc gctgcacgag
     2281 ttcctgccgc acacggcgga ggaaatcgcc gacgtggcca gggccatggg cgccgatccg
     2341 gagcggctgc gccagcgcgc ggcggagctc tccgaatcca accccatgct cggcttccgc
     2401 ggctgccgcc ttgccgtggc ctatccggaa atcgccgaga tgcaggcccg cgccatcttc
     2461 gaggccgccg tcgctgcggc gaagaagacc ggcaagccgg tgacgccgga agtcatggtg
     2521 ccgctggtga tgacaaagcc ggagctcgac cacgtcaagg cgcgcatcga cgccatggcg
     2581 gttgcggtga agagggagac cggcgcggaa ttcgcctatc aggtcggcac gatgatcgag
     2641 ttgccgcgag ccgccctgcg cgccgaggag atcgcccgct cggccgaatt cttctccttc
     2701 ggcaccaatg acctgacgca gacgaccctc ggcatcagcc gcgacgacgc ggcccacttc
     2761 ctcgaaccct acaagcggcg cggcattctt gccgaagacc ccttcatgac cctggaccag
     2821 gatggcgtcg gcgaactcgt caagatcgcc gccgagcgcg ggcgcaaaac ccggccggac
     2881 atcaagctcg gcatctgcgg cgagcatggt ggcgaccccg cctcgatcgg cttttgcgag
     2941 atgaccggcc tcgactacgt ctcctgctcg cccttccgcg tgccggtcgc ccgcctcgcc
     3001 gcggcgcagg cggcgctcgg caaggtcacc aaggcagagg gctgagaagc ggtccggctc
     3061 ggccggatga acaggtccgg tggacccttt cgcgcgacga acgtcctgac ccccgcgatg
     3121 ggccggagca gtcccaattg tgcgccgcaa cggcttaacc ccccggaaac cttgtccggc
     3181 gaagatgcca gcgtgtggag atgcgcctgt ccggggtcag ggattcggta acgaatcgcc
     3241 cggggcgggc gcgaaggttc ggggttcggt aggtgcatcg tccgcgttcg caggcgttca
     3301 ggccgaagaa gacggcgcgc tgggtggcga aagccctggc gccgtggtcc gttgccagct
     3361 gcctgctgat ctcattcacc gccagcgccg gcccgcggat cgatgcgttc gatgcccggc
     3421 gggcgtcgat catcgatctc gcctcggtga ccctgccacc ccggatcgcg cccggccgcg
     3481 aaacaggccc agtcgtaccc ggctactcgg gcctgcgcga agcgccgctg gccgatgtgg
     3541 tgcgatggct ggtgccgccc tcggtcgggg gcct
//
LOCUS       DAOMXN010000237         3566 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_14406_length_3566_cov_9.802051, whole genome shotgun sequence.
ACCESSION   DAOMXN010000237 DAOMXN010000000
VERSION     DAOMXN010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14406_length_3566_cov_9.802051"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="PK812_12690"
     CDS             complement(<1..728)
                     /locus_tag="PK812_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018045566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRJ70451.1"
                     /translation="MRSSPSPDPNPQPARLESGQPARPRLLFVVTEDWFFCSHFLPMA
                     RAAQADGFDVAVACRVRAHRDRLEAEGLHVIAIEAERRSLNPLAVTGAIARLKSAIAA
                     ERPDIVHLIALRAILTGGIAARLAGISRRVVALTGLGFIGAGQGRLAQIARIGVRLAI
                     RWLVDGPRTRFLFENRSDPRLLGLDPDATRKVTIVGGAGVDPEALRPAPLPPMPPLKV
                     ALVARMLWSKGVDVAVDAVRQARRR"
     gene            complement(775..1719)
                     /locus_tag="PK812_12695"
     CDS             complement(775..1719)
                     /locus_tag="PK812_12695"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HRJ70452.1"
                     /translation="MTSIYPFGWKLKLPVIAAPMFLVSGPKLVSACMRAGIVGTWPSL
                     NQRSTEGYVDWINEVREATRDMPAESAIFGVNLIVHGSNTRLQADVMATVANRVPLVI
                     TSLGAVKEIVQAVHSYGGKVFHDVTTRRHAEKAVEAGVDGLILVAAGAGGHAGTANPL
                     ALIAETRRFFAGPIALSGAMSNGAQVAAAIAAGADFAYMGTRFIATEESLAQPDYKAM
                     ITQASIADIVYTPVVSGVPASFMAESLRRAGLDPKNLPAHDPKSHKLDMASEAKAWRD
                     VWSAGHGVADTRDIVPTGDLVARMRREFEAARARLSAY"
     gene            complement(1785..2156)
                     /gene="hisE"
                     /locus_tag="PK812_12700"
     CDS             complement(1785..2156)
                     /gene="hisE"
                     /locus_tag="PK812_12700"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HRJ70453.1"
                     /translation="MTDSINTLYSAILDARSKDPLESRTSKLLREGSAKIAKKLAEEA
                     VEVGHEAILKDAGSVVRESADVIYNLCVLWADVGIKPEDVWSEMERRVKLMGIAEKLP
                     KPPSPYRGRKLRGNGISAAAG"
     gene            complement(2240..2737)
                     /locus_tag="PK812_12705"
     CDS             complement(2240..2737)
                     /locus_tag="PK812_12705"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015950940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HRJ70454.1"
                     /translation="MMPYRPCVGAALFRQDGLVFIGRRKPAKGPEYDAEALDWQMPQG
                     GIDGDEPAETAARRELYEETNVRSVTLIGESPVWFHYDLPDAALGRALQGRFRGQTQK
                     WFAYRFDGKDSEIDVLTPAGGAHGHEFVEWRWERLDRLPALIVPFKRGVYEQIAGAFA
                     GLTGR"
     gene            complement(2778..>3566)
                     /locus_tag="PK812_12710"
     CDS             complement(2778..>3566)
                     /locus_tag="PK812_12710"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HRJ70455.1"
                     /translation="KQLVDKGRHGPLPRIAPDGDRPSDIYARPFDAAKAAGKPKVSVL
                     LTGLGIGASITSEATAKLPGEVSFAFAPYGNDLEKQVARARDDGHEILLQLPMEPFDY
                     PENDPGPQTLLADAPEAQNLDRLHWLMARFQGYAGLTNFMGARLSTSRKGLKPILAEA
                     AKRGLVFVDDGAAGRSQIVTAAGEVGLPAARGDIVLDGIDKPADLDAALARAENLART
                     NGRVIVMGPALPMTVERLGRWLKTLEQKGIVLAPVTAQVAAKKG"
BASE COUNT          617 a         1175 c         1174 g          600 t
ORIGIN      
        1 cgccgccgcg cttgccgaac cgcatcgacc gccacgtcca cgcccttgct ccagagcatc
       61 cgggcgacca gcgccacctt cagggggggc atcggaggca gcggggcggg cctgagcgcc
      121 tcgggatcga cgccggcgcc gccgacgatc gtcaccttcc gtgtggcgtc gggatcaagg
      181 ccgagcagac gcgggtcgct gcggttctcg aagaggaagc gcgtgcgtgg gccatccacc
      241 agccagcgga tcgccagccg gacgccgatc cgcgcgatct gtgcgagccg gccctgtccg
      301 gctccgatga agccaaggcc tgtcagtgcg accacgcgcc gagaaattcc ggcgagccgc
      361 gccgcgatcc cgccggtcag gatggcgcgc aaggcgatga gatgcacgat gtcggggcgt
      421 tccgccgcga tggccgactt cagccgggcg atggcgccgg tcacggcgag agggttgagc
      481 gagcggcgct cggcctcgat ggcgatgacg tgcaggcctt cggcttccag acggtcgcga
      541 tgcgcgcgca cgcggcaggc cacggccacg tcaaagccgt cggcttgcgc ggcccgcgcc
      601 atgggcagga aatgcgaaca gaagaaccag tcctccgtca ccacgaacag gagacggggt
      661 ctggccggtt gcccgctttc caggcgcgca ggttggggat tcgggtccgg ggaaggcgag
      721 gagcgcatgg cgacgctcct taaacggggt tgcgcgaatt cgcaaaaaac gccgtcaata
      781 ggccgacagc ctcgcgcgtg ccgcctcgaa ttcgcgacgc atgcgcgcca cgagatcgcc
      841 tgtggggacg atatcgcgcg tgtccgcgac gccgtggccg gctgaccata cgtcgcgcca
      901 ggccttggcc tcgcttgcca tgtcgagctt gtggctcttg ggatcatgag ccggcaggtt
      961 cttgggatcg agaccggcgc ggcgcagcga ttcggccatg aagctcgccg gcacgcccga
     1021 gaccaccggg gtatagacga tatcggcgat cgaggcctga gtgatcatcg ccttgtagtc
     1081 gggctgggcc agcgattcct ccgtggcgat gaagcgcgtc cccatgtagg cgaagtcggc
     1141 gccggcggcg atcgcggccg cgacctgcgc gccattcgac atcgcgccgg agagcgcgat
     1201 cggccccgca aagaagcgcc gcgtttcggc gatcaacgca agcgggttgg ccgtgccggc
     1261 atgaccaccc gcgccggcgg caaccaggat caggccgtcc acgccggcct cgacggcttt
     1321 ttccgcatgc cgacgggtcg tgacgtcatg aaagaccttg ccgccgtagc tgtgaacagc
     1381 ctggacaatc tccttcaccg cgccgagcga ggttatgacc agcggcaccc tgttggcgac
     1441 cgtggccatc acatcggcct ggaggcgggt gttggagccg tgcacgatca gattgacgcc
     1501 aaagatcgcg ctctcggccg gcatgtcgcg agtcgcctcg cgcacctcgt tgatccagtc
     1561 gacataccct tcggttgagc gctggttcag cgacggccag gtgccgacaa tgccggcgcg
     1621 catgcaggcg ctgaccagtt tcggtccgct gacgaggaac atcggcgcgg cgatgacggg
     1681 aagcttcagt ttccagccga agggatagat cgatgtcatg gaggcaccct ttcaggatca
     1741 ggtcccgaaa aggttatatg taaactatca atgagggaag gggcttaccc cgcagcggcg
     1801 ctgatgccgt tgccgcgcag cttgcgaccg cggtacggcg agggcggctt gggcagcttt
     1861 tcggcgatgc ccatcagttt cacccgccgc tccatttccg accagacatc ttccggtttg
     1921 atgccgacgt cggcccagag cacacacaga ttgtaaatga catcggcact ctcgcgcacc
     1981 accgagccgg catccttgag gatggcttca tgaccgacct cgaccgcttc ctcggcgagc
     2041 ttcttggcga tcttggccga tccctcgcgc aggagcttgg acgtgcgcga ttccagcggg
     2101 tccttcgaac gcgcgtcaag aatggcagaa tacagcgtgt tgatggaatc ggtcatccgg
     2161 gatctccggt cagattcagg catcatgcaa cattttgcca acaataacat gacaaatatg
     2221 aaaggcggct aaacaatttt cagcgaccgg tcaggcccgc gaaagcaccc gcgatctgtt
     2281 catagacgcc gcgcttgaag ggtacgatca gtgccggaag ccggtcgagc cgttcccagc
     2341 gccattcgac aaactcatgg ccatgggcgc cgccggccgg cgtcaggacg tcgatctcgc
     2401 tgtccttgcc gtcaaaacga taggcgaacc atttctgagt ctgaccgcga aagcggccct
     2461 gcagcgcgcg gccgagagcg gcgtcgggca ggtcatagtg aaaccagaca ggcgattcgc
     2521 cgatcagggt caccgagcgg acgtttgtct cctcgtacaa ttcgcgccgc gcggccgttt
     2581 ccgccggttc atcgccgtcg atgccgccct ggggcatctg ccagtccagc gcctcggcgt
     2641 cgtactccgg ccccttggcg ggtttgcgcc ggccgatgaa caccaggccg tcctgcctga
     2701 acagggcggc gccaacgcag ggacgatacg gcatcatcgc acgaaccgga gcgtgtccga
     2761 ccggcttttt cacggcttca ccccttcttg gcggcgactt gtgcggttac cggagccagc
     2821 acgatgccct tctgttccag tgtcttcagc cagcgcccaa gccgttccac cgtcatcggc
     2881 agggccggtc ccatgacgat gacacggccg ttcgtgcgcg caaggttttc cgcccgcgcc
     2941 agcgcggcat cgagatcggc gggcttgtcg atgccatcga gcacgatgtc gccgcgcgcc
     3001 gccggcaggc caacctcgcc cgcagcagta acgatctggc tgcgcccggc cgcgccatca
     3061 tcgacaaaca ccaggccgcg tttcgcggct tcggcaagaa tgggcttgag acccttgcgc
     3121 gatgtcgaca gccgcgcgcc catgaagttc gtcagtccgg catagccctg gaaccgtgcc
     3181 atcagccagt gcaaccggtc gaggttctgg gcctcgggcg cgtcggccag cagggtctgc
     3241 ggaccggggt cgttctcggg atagtcgaac ggctccatgg gcaactgaag caggatctcg
     3301 tggccgtcat cacgcgcccg cgcgacctgc ttttcaagat cgttgccgta gggagcgaag
     3361 gcgaagctga cctcgccggg aagcttggcc gtcgcctcgg atgtgatgct cgcgccgatg
     3421 cccagaccgg tcagcagaac cgataccttt ggcttgccgg cggccttggc ggcgtcgaac
     3481 ggccgcgcat agatgtcgct cggccggtcg ccatcgggcg caattcgtgg cagcggcccg
     3541 tgccgcccct tgtcgaccag ctgctt
//
LOCUS       DAOMXN010000238         3537 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_14607_length_3537_cov_10.731189, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000238 DAOMXN010000000
VERSION     DAOMXN010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14607_length_3537_cov_10.731189"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1591)
                     /locus_tag="PK812_12715"
     CDS             complement(<1..1591)
                     /locus_tag="PK812_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003600828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HRJ70456.1"
                     /translation="MTLDPGLPAPAGLYDPRFEKDSCGVGFIADMKNRKSHAIIEQGL
                     RILHNLDHRGAVGADPKAGDGCGMLVQIPHRFFKEECAGLGFDLPEPGHYAILQLFLP
                     RDKAGRKTVQKIVDKTIKAEGQKVLGWRDVPVDNSDLGESVLPTEPVHRQVFIGRAAN
                     IATEDEFERRMFILRKVVSNKIYDLKDPATAGYYPVSSSCRTVVYKGMLLATQLGSYF
                     NDLNDPRFESALTLVHQRFSTNTFPTWSLAHPYRMVAHNGEINTLRGNVNWMAARQAS
                     VDSELFDSEISKLWPISYEGQSDTACFDNALEFLVQGGYSLAHAMMMLIPEAWAGNPL
                     MDEQRRAFYEYHAALMEPWDGPAAVAFTDGRQIGATLDRNGLRPARYLVTDDDLVVMA
                     SEMGVLPIPEEKIVTKWRLQPGKMLLIDLEQGRIISDEEIKKELSGANPYRDWLKRTQ
                     IVLEDLKPVEPRASRTDVSLLDKQQAFGYTQEDLAFLMAPMAVTGQEAVGSMGTDTPI
                     SALSDKSKLLYTYFKQNFAQVT"
     gene            1992..3068
                     /locus_tag="PK812_12720"
     CDS             1992..3068
                     /locus_tag="PK812_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HRJ70457.1"
                     /translation="MAGRVLNFASDNVVGASDKVLQAIVSANGGALASYGADTITKRV
                     EHRLNALFERDCQTFLVTTGTAANALAIACLSPPHGAVLCHEEAHSIDDECGAPEFFM
                     GGGKLVGIAGAGAKLTRKAILDHLAAEPGGTRHPPIRGLSISQATECGTVYSLAEIAE
                     VSRAAHDNGMHVHMDGARFANALLHLGCAPAEMTWKAGIDILSFGGTKNGCLMAEAVV
                     FFNPALAEDFAFKRKRAGQTVSKGRLLAAQFDAYLGDDHWLDLARHANAMADRLAGGL
                     TAIPGLRLAWPTDANEVFCIMPEALYESLKARDVVFYDWTDKSLPRDNRLKKGEVVTR
                     LICSFATAQKDVDALLRLARTLAG"
     gene            3148..>3537
                     /locus_tag="PK812_12725"
     CDS             3148..>3537
                     /locus_tag="PK812_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70458.1"
                     /translation="MGHRGKPHRWMGCAAGALALAGLGAPAQAQYFFGPPVLPQRAVI
                     EVLRDEGFRRISPPILNRDVYFLDAVDPYGTPVRLIVSAYNGDIIKAHLRPRGAWQEA
                     DPGWDSWDEPPPRRAHPAERGQKQAVAP"
BASE COUNT          622 a         1158 c         1137 g          620 t
ORIGIN      
        1 tcgtgacctg cgcgaaattc tgcttgaagt aggtgtagag cagcttcgac ttgtcggaga
       61 gcgccgagat cggcgtgtcc gtgcccatcg atccgaccgc ttcctggccg gtgaccgcca
      121 tgggcgccat gaggaacgcc agatcctcct gcgtgtagcc gaaagcctgc tgcttatcga
      181 gcagcgagac gtccgtgcgc gaggcacgcg gctcgacggg cttcaaatcc tcgagcacga
      241 tctgggtgcg cttcagccag tcgcgatagg ggtttgcgcc ggagagttcc ttcttgatct
      301 cctcatcgga gatgatgcgg ccctgctcaa gatcgatcag cagcatcttg cccggctgga
      361 gacgccactt ggtgacgatc ttctcctccg gaatcggcag gacgcccatc tcggaggcca
      421 tgacgaccag gtcatcgtcg gtcacgaggt agcgcgcggg cctgaggccg ttgcggtcca
      481 gcgtcgcgcc gatctggcgg ccatcggtga aggcaaccgc cgcaggtccg tcccacggct
      541 ccatcagcgc ggcgtgatac tcgtagaagg cgcgccgctg ctcatccatc agcgggttgc
      601 cggcccacgc ttccggaatc agcatcatca tggcgtgggc cagcgagtac ccgccctgca
      661 ccaggaattc gagcgcgttg tcgaaacagg cggtgtccga ctggccttca taggagatcg
      721 gccagagctt ggagatttcc gaatcgaaca gttccgaatc caccgaggcc tgacgcgccg
      781 ccatccagtt gacgttgccg cgcagggtgt tgatctcgcc gttgtgcgcg accatgcgat
      841 agggatgggc cagcgaccag gtcgggaagg tgttggtcga gaagcgctgg tggacgaggg
      901 tgagcgcgct ttcgaaacgc ggatcgttca ggtcgttgaa atagctgccg agctgcgtcg
      961 ccagcagcat gcccttgtag accaccgtgc ggcaggacga ggacaccgga tagtaccccg
     1021 ccgtcgccgg gtccttcagg tcgtagatct tgttggagac gaccttgcgc aggatgaaca
     1081 tccggcgttc gaactcgtcc tcggttgcga tgttggcggc ccgtccgatg aagacctgac
     1141 gatgcaccgg ctcggtcggg agcacgctct cgcccagatc cgaattgtcg accggcacgt
     1201 cgcgccaacc cagcaccttc tgaccctcgg ccttgatcgt cttgtcgacg atcttctgca
     1261 cggtcttgcg cccggccttg tcgcgcggca ggaacaactg gagaatggcg taatgccccg
     1321 gctccggcag gtcgaagccc agcccggcgc actcctcctt gaagaagcga tgcgggatct
     1381 gcaccagcat gccgcagcca tcgccggcct tcgggtccgc gcccaccgcg ccgcgatgat
     1441 cgaggttgtg caggatgcgc aacccctgct cgatgatggc atggctcttg cggttcttca
     1501 tgtcggcgat gaagccgacg ccgcaggaat ccttttcgaa ccggggatca tagagtccgg
     1561 cgggcgcggg caggccggga tcgagggtca tcggcagctt tcgctccggt cccggggttg
     1621 gagtgaggac cgccgaagca acatcggcgc gatccgaaac gatcccggtc atgacttacc
     1681 tctttacttt ccaaccgcca ccccggcggt tacaggaacc ggcacatccc tccgcaccct
     1741 cgcggagccg cttccaggac aggcaccttc acccggaagg tcaatatagc tgtcctaaat
     1801 gctccggcag ctaaaacaga aaaggctgtg cagcacaagc gtccgctgga ctgtttcagc
     1861 cggaatgttg cgcatttgca cggtttcacc ccgtcccgcc gcggcgcggt ccggatcacg
     1921 cgaagcgact tgaaattcat ccggcggggg agtccagtgg cagacggcag ctgaagtcga
     1981 aggaatcccg catggcagga agagttttga atttcgcaag cgacaatgtg gtcggcgctt
     2041 cggacaaggt attgcaggcg atcgtctcgg ccaatggcgg cgcgctcgcc tcctacggcg
     2101 ccgacaccat caccaaacgc gtcgaacacc ggttgaacgc gcttttcgag cgggattgcc
     2161 aaaccttcct ggtgacgacc ggaacggcgg cgaacgccct ggcgatcgcc tgcctgtcgc
     2221 cgccgcatgg cgcggtgctg tgccacgagg aggcccattc catcgacgat gaatgcggcg
     2281 cgccggaatt tttcatgggc ggcggcaagc tcgtcggcat cgcgggcgca ggcgcgaaac
     2341 tgacgcgcaa ggccatcctc gaccatctcg ccgcggagcc cggcggcacc cgccatccgc
     2401 cgatccgcgg cctgtcgatc tcgcaggcga ccgaatgcgg gaccgtctat tccctggcgg
     2461 aaatcgccga agtcagccgc gccgcgcatg acaatggcat gcatgtgcat atggacggcg
     2521 cgcgcttcgc caatgcgctc ctgcacctcg gctgcgcgcc ggcggagatg acctggaagg
     2581 cggggatcga catcctgtcc ttcggcggca ccaagaacgg ctgcctgatg gccgaggccg
     2641 tggtgttctt caacccggcg ctggccgagg acttcgcctt caagcgcaag cgtgccggcc
     2701 agaccgtctc gaagggccgg ctgctggccg cgcaattcga cgcttacctt ggcgatgacc
     2761 actggctcga ccttgcccgg cacgccaacg ccatggcgga caggcttgcc gggggtctga
     2821 ccgcaattcc cggcctgcgc ctcgcctggc cgaccgacgc caacgaggtc ttctgcatca
     2881 tgccggaagc gctctatgaa agcctgaagg ccagggacgt cgtgttctac gactggaccg
     2941 acaagagcct gccccgggac aaccgtctga aaaagggcga ggtcgtcacg cggctgatct
     3001 gctccttcgc caccgcgcaa aaggacgtgg atgccctcct tcggctggcg cgcacgctgg
     3061 ccggctgaac gcggcgttcc gccctaggat tgtcgcattc gccggctctt ttcgctccgg
     3121 cggcgcaatc aagacaggcg gaagtccatg ggacacagag gcaaaccaca tcgctggatg
     3181 ggatgcgcgg ccggcgcgct ggcgctcgcg ggccttggcg ctccggcaca ggcgcagtat
     3241 ttcttcggcc cgccggtgct accgcagcgc gccgtgatcg aggtgctgcg cgacgagggg
     3301 tttcgccgca tctcgccgcc aatcctcaac cgcgatgttt atttcctcga tgccgtggac
     3361 ccttacggaa caccggtgcg gctgatcgtc agcgcctaca acggagacat catcaaggcg
     3421 cacctgcgcc cccgtggcgc ctggcaggag gccgatccgg gctgggacag ctgggatgag
     3481 cccccgccgc gccgcgcgca tcccgccgag cgtggccaga aacaggctgt ggccccg
//
LOCUS       DAOMXN010000239         3527 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_14667_length_3527_cov_11.974366, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000239 DAOMXN010000000
VERSION     DAOMXN010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14667_length_3527_cov_11.974366"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..509)
                     /locus_tag="PK812_12730"
     CDS             complement(12..509)
                     /locus_tag="PK812_12730"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRJ70459.1"
                     /translation="MRFGISGLAAVAAVVGTLGLAGCQSSAPTMASADVTNAPVGAGV
                     NVSPGSREDFIVHIGRRVFFGEGSADLDGTDLETVTLQAAWLARYTSYKVRVQGHADD
                     RGSAQFNKDLSMRRAEAVKAALASRGVDPSRITTQGVGNTRPQLRCQDISCWAQNRRV
                     LVQLM"
     gene            complement(537..1205)
                     /locus_tag="PK812_12735"
     CDS             complement(537..1205)
                     /locus_tag="PK812_12735"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017967731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRJ70460.1"
                     /translation="MLVSRRNVLRIGAGGSALLTAPAVFAAKARHDPPKHTGGSEESY
                     FGPEVEDRGFKFRSTRMEYIDPQFRRQLVKYKHKEPVGSIVIDTAAHYLYITFENNTA
                     LRYGVGVGREGFQWFGRAQVGRKAEWPRWVPPAEMRARRPDLPESMEGGPGNPLGPRS
                     HYLYRDGRDLIYRIHGTTEPWSIGHDVSSGCIRLLNEDVIDLYRRVPVGTRVLVLKHL
                     GQPV"
     gene            complement(1303..1977)
                     /locus_tag="PK812_12740"
     CDS             complement(1303..1977)
                     /locus_tag="PK812_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008839476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HRJ70461.1"
                     /translation="MMTLPQTIKAFAFDAYGTVFDFHGAVTRHRAAAGPDAEAFSQLW
                     RQKHLEYAWTRTLMGRYVDFWTLTQQSLDYCFARFPSVPGTLRQSLLDAYFRLDAFAD
                     AAPLLNRLQQAGLRCVLFTNGTPAMAQAAAESAGLAPYFETIVSVDEIGAFKSVPEVY
                     GLLHSRLGLNRGEIALVSSNRWDVAGGVSYGMPAVWANRTGQPDEYADLPPTLTVTSL
                     AEINLS"
     gene            2176..3033
                     /locus_tag="PK812_12745"
     CDS             2176..3033
                     /locus_tag="PK812_12745"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008190784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HRJ70462.1"
                     /translation="MKSWSETWTWYDGEWLGGNPGLTGPRSHAFWLSSSVFDGARVFD
                     GVMPDLDLHCARLNRSAVALGLKATRTVAEMVALTQEGVKKFASGTALYVKPMYWAEF
                     EGGNAILPDAETTRFCLCLFAAPMPEPTGWSVTLSPYRRPTIENAPTDSKAGCLYPNN
                     ARALREAQARGFANALVRDMLGNIAETCTSNVFMAKDGVVFTPLPNGSFLNGITRQRT
                     IALLRADGIEVVEGSLTYADFEKADEIFSSGNYSKVVPITRIDGRDLQPGPLMRRARS
                     LYMDFAHST"
     gene            3039..>3527
                     /locus_tag="PK812_12750"
     CDS             3039..>3527
                     /locus_tag="PK812_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HRJ70463.1"
                     /translation="MLATRLTERFGLRHPIVSAPMAFAGGGALAAAVTAAGGLGLIGG
                     GYGDPGWIEEQFKAAGNQKVGCGFITWALRKSPSLLSDVLKHRPAAIMLSFGDPAPYA
                     EEIRAAGAALICQCQTLDHVEDAIAAGADIIVAQGGEAGGHGMARGTLTLVPEAADRI
                     AAR"
BASE COUNT          666 a         1164 c         1101 g          596 t
ORIGIN      
        1 ggcttctgcc ctcacatcaa ctgcaccagg acacggcggt tctgcgccca gcaggagata
       61 tcctggcacc gcagctgcgg acgggtattg cccaccccct gcgtggtgat gcgcgaaggg
      121 tcgacccccc tggacgccag cgccgccttg acggcctcgg cgcggcgcat ggacagatcc
      181 ttgttgaact gggccgagcc gcgatcgtcg gcatggcctt gcacacgcac cttgtaggag
      241 gtgtaccgcg cgagccaggc cgcctgcagg gttacagttt caagatcggt gccgtcgagg
      301 tcggcggagc cttcaccgaa gaagacgcgc cgcccgatat gcacgatgaa atcctcccgc
      361 gatccgggcg agacattgac gcccgcgcca acgggcgcat tcgtcacatc agcactcgcc
      421 atcgtcggcg cagaactctg acacccggca agccccaggg tcccgacaac cgccgccacg
      481 gccgccaggc cggaaatacc aaaacgcatg tccgcaactc caagactgct tgttgatcag
      541 accggttggc caaggtgctt gagcacgagc acgcgcgtgc ccaccggaac gcggcggtag
      601 aggtcgatca cgtcttcgtt cagcaggcgg atgcagccgg acgacacgtc atggccgatg
      661 ctccacggct cggtggtgcc gtgaatgcgg tagatgaggt cgcgaccgtc gcgatagaga
      721 taatgggacc gcggcccaag cgggtttcca ggcccacctt ccatcgactc cggcaaatcc
      781 gggcgacgcg cgcgcatttc cgccggcggc acccagcgcg gccattccgc cttgcggccg
      841 acctgggccc ggccgaacca ctggaagccc tcgcgaccga cgcccacgcc atagcgcagg
      901 gcggtgttgt tctcgaaagt gatataaaga taatgcgcgg ccgtatcgat aacgatgctg
      961 cccaccggct ccttgtgctt gtacttcaca agctgccgcc gaaactgcgg atcgatatac
     1021 tccatacgcg tcgaacggaa cttgaagccg cgatcctcaa cttctgggcc gaaataggat
     1081 tcctcgctac cgcccgtgtg cttcggcgga tcgtggcgcg ccttcgccgc aaataccgct
     1141 ggcgcggtca aaagcgccga accaccagcg ccgatacgca aaacatttct tctgctgacc
     1201 agcatgacac cctcccgata gtcggcgaga catccccgtt aacgattaaa gccctgaaac
     1261 cagcgcgtgg caagacgccg cgccccgggg ttcaaccaat cgttaactaa ggttaatttc
     1321 ggccaacgat gtgacggtaa gcgtcggcgg cagatccgca tactcgtccg gttggccggt
     1381 acggttcgcc cagaccgccg gcatgccgta ggacacgccg cccgccacat cccagcggtt
     1441 ggacgacacg agcgcaatct cgccgcgatt caggccaagt ctgctatgga gcaacccgta
     1501 gacctcaggc actgacttga aggccccgat ctcatcgacg gaaacgatgg tttcgaaata
     1561 cggtgcaagg ccggcacttt cggcagcggc ctgtgccatc gccggcgtcc cattggtgaa
     1621 caggacgcag cgcagtccgg cctgctgcaa gcgattcagg agcggcgcgg cgtccgcgaa
     1681 ggcatcgagc cggaaatacg catcgagaag cgactggcga agcgttcccg gcaccgatgg
     1741 gaaccgcgca aaacaataat ccagcgactg ctgcgtcaag gtccagaagt cgacgtaccg
     1801 gcccatgagc gtgcgggtcc aggcatattc cagatgcttc tgacgccaga gctgggaaaa
     1861 ggcttcggca tccggaccgg ctgccgcgcg atggcgcgtg acggcgccat ggaaatcgaa
     1921 caccgtgcca taggcatcga acgcgaaggc cttgatcgtc tgcggcaggg tcatcattct
     1981 cccggcagcg ttcgtcgaac acccaccggc ctttcctgtt ttcggcatgc aggctgcggt
     2041 aggcccggcc tctcgtcaat cacccttcaa accataaccg ccgctactga aaggcatcac
     2101 gaaagactat gtcagcacta ttgacctttt cgacaagggc gcgtccaatg cgcatcgaac
     2161 atgcggagga agcccatgaa atcctggtcg gaaacctgga cttggtatga cggcgaatgg
     2221 cttggtggca atcctggcct gaccgggccg cgcagtcacg ccttctggct gtcgtcttcc
     2281 gttttcgacg gcgcgcgcgt ttttgatggc gtcatgcccg acctggacct gcattgcgcg
     2341 cggctcaacc gctcggctgt ggcgcttggc ctcaaggcga ccaggaccgt cgcggagatg
     2401 gtggcactga cacaggaggg ggtgaagaag ttcgcgtccg gcacggcgct ctatgtcaaa
     2461 ccgatgtact gggctgaatt cgaaggtggc aacgccatct tgcccgatgc tgaaaccacg
     2521 cgcttctgcc tgtgcctgtt cgccgcgccg atgccggaac cgacgggctg gagcgtgacg
     2581 ctctcgccgt accggcggcc gaccatcgag aatgcaccca ccgattccaa ggccggatgc
     2641 ctctatccca acaacgcccg tgccctgcgt gaggcgcagg cgcgcggctt cgccaatgcg
     2701 ctggtgcgcg acatgctggg caacatcgcc gagacctgca cctccaacgt gttcatggcg
     2761 aaggacggcg tggtgttcac gcctttgccc aacgggtcgt ttctcaacgg catcacccgc
     2821 cagcgcacca tcgcgcttct gcgggcggac gggatcgagg tggtggaggg ctcgctcacc
     2881 tacgcggatt tcgagaaggc ggacgagatt ttctcctccg gcaactattc caaggtggtg
     2941 ccgatcaccc gcatcgacgg acgcgatctg cagccggggc cgctgatgcg ccgggcacgg
     3001 tcgctctaca tggacttcgc gcattcgacg tgaccgacat gctggcgacc cgcctgaccg
     3061 aacgcttcgg cctgaggcac cccatcgtct ccgcgcccat ggccttcgcc gggggcggcg
     3121 cgctcgcggc ggcggtgacg gcagccggcg gcctcgggct gatcggcggg ggatacggcg
     3181 atcccggctg gattgaggag cagttcaaag ccgcaggaaa tcaaaaggtt ggctgtggct
     3241 tcatcacctg ggcgttgcgc aagtcgccgt cgctgctgag cgatgtcctc aagcacaggc
     3301 cggcggcgat catgctgtcc ttcggcgatc ccgcccccta cgccgaggag atccgggccg
     3361 ccggcgctgc cttgatctgc cagtgccaga cgctcgacca tgtggaggat gccatcgcgg
     3421 cgggcgccga catcatcgtg gcgcagggcg gcgaagctgg cgggcatggc atggcgcgcg
     3481 gcacgctgac tctcgttccc gaagcggcgg accgcatcgc cgcgcgc
//
LOCUS       DAOMXN010000240         3499 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_14827_length_3499_cov_10.739547, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000240 DAOMXN010000000
VERSION     DAOMXN010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14827_length_3499_cov_10.739547"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="PK812_12755"
     CDS             <1..622
                     /locus_tag="PK812_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003600912.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HRJ70464.1"
                     /translation="NLLDLGVKAARAKATVGEISLALEKAWGRHVAEIKAISGVYKRE
                     VGVNSEMIAKVQRKVEAFEEADGRRPRILVAKLGQDGHDRGQKVIASAFADLGFDVDI
                     GPLFATPEEAARQAVENDVHIVGVSSLAAGHLTLVPAIRDALAKEGRPDIMIVAGGVI
                     PPQDYEALYKAGAAAIFPPGTAIADAAAKLLDDLSDRLGYRQKAAG"
     gene            complement(769..1305)
                     /locus_tag="PK812_12760"
     CDS             complement(769..1305)
                     /locus_tag="PK812_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HRJ70465.1"
                     /translation="MTPQDVTGFWLQAGPKRWFARDAAFDAEIRRRFGAAVEDAREGR
                     LDSWADSDDGALALVILLDQFSRNIHRGSPLAFAGDDKARAVAEAVIARGGLDGLPTE
                     KAQWFILPFEHHEDMGSQERAIRLFEALGDAELVKWAVVHRDIIAMFGRFPHRNAALG
                     RETTAEEAAFLAEGGFAG"
     gene            1417..1950
                     /locus_tag="PK812_12765"
     CDS             1417..1950
                     /locus_tag="PK812_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HRJ70466.1"
                     /translation="MINPLIIAAVETRPGWGRLLISRCPGRTDLDHDLDYDLDAIRRA
                     GASAVVTLVSDEELDELGVAAMGAAVRQRDMEWFHLPIEDYNTPDDEWERRWTESGAQ
                     LRALIRGGRTIHLHCRGGCGRAGMTAARLLTELGAEPAEAIARVRGARPCAVETEAQE
                     AAVMAVQPQADIDATLA"
     gene            1961..2716
                     /locus_tag="PK812_12770"
     CDS             1961..2716
                     /locus_tag="PK812_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009463924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRJ70467.1"
                     /translation="MAIYPDLNGKTVLVTGGGSGIGASIVEHFARQGSKVGFIDIAEA
                     PSRRLAADLTAAGHAVRFELADLTDIAALRQAIANVRAAFGPITILVNNAAHDDRHPT
                     ETVTPEYFDDRIAVNFKHQFFAAQAVLADMKAAGGGAIVNFSSVSWVAGMGGMAVYTA
                     AKSAVVGLTRSLARDYGPFNIRVNAIAPGWILTERQVTKWMTPEGEKALFERQSLKRK
                     LFPPDIARVVLFMASQEASAISGQHYVVDGGWT"
     gene            2828..>3499
                     /locus_tag="PK812_12775"
     CDS             2828..>3499
                     /locus_tag="PK812_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009494558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HRJ70468.1"
                     /translation="MTVRIAAALAATLLAGSALAQQPIKPAVTFDVGGKFDKSFNEAS
                     YNGAEAFKKKTGIAYREFEVQNDAQREQALTRFARDGNDPIVVTGFSNGAALKKVAAE
                     FPKTRFTIIDFVVDLPNVQSIVFKEHEGSYLVGIAAAMASKSNKVGFVGGMDIPLIRK
                     FACGYVAGVKAAKPGAEVFQNMTGTTGAAFNDPVKGAELARSQMDRGADVVYHAAGGT
                     GAGVLR"
BASE COUNT          604 a         1172 c         1172 g          551 t
ORIGIN      
        1 caacctgctg gacctcggcg tcaaggctgc ccgggccaag gccacggtcg gcgagatcag
       61 cctggcgctg gaaaaggcct ggggccggca tgtggcggag atcaaggcca tttccggcgt
      121 ctacaagcgg gaggtcggcg tgaattccga gatgatcgcc aaggtgcagc gcaaggtgga
      181 agccttcgag gaggcggacg ggcgccgccc gcgcatcctc gtcgccaagc tcgggcagga
      241 cggccacgac cgcggccaga aggtgatcgc ctcggccttc gccgatttgg gtttcgacgt
      301 cgacatcggc ccgctgttcg cgacgccgga ggaggccgcg cggcaggccg tcgagaacga
      361 cgtgcacatc gtcggcgtgt cctcgctcgc cgccgggcac ctgacgctcg tccccgccat
      421 ccgcgatgcg ctggcgaaag aggggcggcc cgatatcatg atcgtcgccg gcggcgtcat
      481 cccgccgcag gattacgagg cgctctacaa ggccggcgcg gcggcgatct tccctcccgg
      541 cacggctatc gccgacgcgg cggcgaaact gctcgatgat ctttcagacc ggctggggta
      601 tcggcagaag gcggcggggt gagggggcgc aagtgaggat gccattgata tcctgtcccc
      661 gtaatcggcg ccgatgcttg caatcccgta atgtttgagt tcccgccggt ccgcagcgcc
      721 gctggccgaa agccggtctg ctgatcgcca actcgccttt gcgcacccct accccgcgaa
      781 gccgccctcg gccagaaacg cggcctcttc cgccgtcgtt tcgcgcccaa gcgcggcgtt
      841 gcggtggggg aaacggccga acatcgcgat gatgtcgcga tgcaccacgg cccatttgac
      901 gagctccgcg tcgcccagag cctcgaacag ccggatcgcc cgctcctgcg agcccatgtc
      961 ctcgtggtgc tcgaagggca ggatgaacca ttgcgccttc tcggtcggca gtccgtcgag
     1021 cccgccgcgc gcgatcacgg cctcggcgac ggcacgggcc ttgtcgtcgc cggcgaacgc
     1081 gagcggagag ccgcgatgga tattgcgcga gaactgatcg agcaggatca ccagcgccag
     1141 cgcgccgtcg tcgctgtccg cccaggaatc gagcctgccc tcgcgcgcgt cctccaccgc
     1201 cgcgccgaag cgccgacgaa tttccgcgtc gaaggccgcg tccctggcga accagcgctt
     1261 cggtccggcc tgaagccaga aaccggtcac gtcctgcggc gtcatggacc ctccccaaag
     1321 tgcctgctcg actgccatcg tcatcgcact gtgccacggt cagcgccgac gcgcacctta
     1381 ttcccccggc gtttcagccg tgctacctgc cgatccatga tcaatccgct catcatcgcc
     1441 gcggtggaaa cgcggcccgg ctggggaagg ctgctgatca gccgttgccc ggggcgcacc
     1501 gatctcgatc acgacctcga ctatgatctc gacgcgatcc gccgggccgg cgcgagcgcc
     1561 gtggtcacgc tggtgtcgga cgaggaactg gacgaactcg gcgtcgcggc aatgggggcg
     1621 gcggttcgcc agcgcgacat ggaatggttt catctgccga tcgaggatta caacacgccg
     1681 gatgacgaat gggaacggcg ctggacagag tcgggcgcgc agctccgcgc attgatccgg
     1741 ggcggcagga caatccatct gcattgccgg ggcggatgcg gccgggcggg catgacggcc
     1801 gccaggctgc tgaccgagct tggagccgag ccggcggaag ccatcgcccg cgtgcgcggc
     1861 gcgcgcccct gcgcggtcga aacggaagcg caggaggccg ccgtcatggc ggtgcagccc
     1921 caggcggata tcgacgcaac ccttgcgtga ggatcagagc atggccatct accccgacct
     1981 gaatggaaag accgtgctgg tcaccggcgg cggatcaggc atcggtgcct cgatcgtcga
     2041 gcatttcgcc cggcagggat cgaaggtcgg tttcatcgac atcgccgagg cgccgtcgcg
     2101 ccggctggcc gccgacctca ccgcagccgg ccatgccgtg cggttcgaat tggccgacct
     2161 caccgacata gccgcgttgc gccaggcgat cgcaaacgtc cgggcggcat tcggccccat
     2221 caccattctc gtcaacaacg ccgcgcacga cgaccgtcat ccgaccgaga cggtgacgcc
     2281 ggagtatttc gatgaccgga tcgcggtgaa tttcaaacac cagttcttcg cggcgcaggc
     2341 ggtgctggca gacatgaaag ccgccggcgg cggggccatc gtcaatttct cgtccgtctc
     2401 ctgggttgcc ggcatgggcg gcatggcggt ctacaccgcc gcgaaatcgg ccgttgtcgg
     2461 actgacgcgg tcgcttgcgc gtgactacgg tccgttcaac atccgggtga acgcgatcgc
     2521 gccaggctgg atcctgaccg agcgccaggt gaccaaatgg atgacgcctg aaggcgagaa
     2581 ggcgctgttc gaacgccagt cgctcaagcg caagctgttc ccccccgaca tcgcccgcgt
     2641 ggtgctgttc atggcctcgc aggaggcttc cgccatcagc ggacagcact atgtggtgga
     2701 tggtggctgg acctgagtat tggcggtgca ggactggacg aggctctgga ctctgccgca
     2761 caccgtgcca tcttgaacac catgcgccgt ccgaacaccg gaccgcgaag acagggagaa
     2821 gagacaaatg accgttcgca tcgctgctgc gctggcagca acccttcttg ccggcagcgc
     2881 gctggcgcag cagccgatca agccggccgt gacgttcgac gtcggcggca agttcgacaa
     2941 gtcattcaac gaggcctcct acaacggcgc cgaagccttc aagaagaaga ccggcatcgc
     3001 ctatcgcgag ttcgaggtgc agaacgatgc gcagcgcgaa caggcgctga cgcgctttgc
     3061 ccgcgacggc aacgatccga tcgtcgtcac cggcttttcc aacggcgcgg cgctgaagaa
     3121 ggtcgcggcg gagtttccga agacccgatt cacgatcatc gacttcgtgg tcgacctgcc
     3181 caacgtgcag tcgatcgtgt tcaaggagca tgagggctcc tatctcgtcg gcatcgcggc
     3241 ggcgatggcc tcgaaatcga acaaggtcgg cttcgtcggg ggcatggata tcccgctgat
     3301 ccgcaagttc gcctgcggct atgtcgccgg cgtcaaggcg gccaagccag gcgcggaagt
     3361 gttccagaac atgaccggca ccaccggcgc ggccttcaac gatccggtga agggcgcgga
     3421 gctcgcccgc tcgcagatgg atcgcggcgc ggacgtggtc taccacgcgg cgggcggcac
     3481 cggcgccggc gtcctgcgc
//
LOCUS       DAOMXN010000241         3488 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_14894_length_3488_cov_9.850568, whole genome shotgun sequence.
ACCESSION   DAOMXN010000241 DAOMXN010000000
VERSION     DAOMXN010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14894_length_3488_cov_9.850568"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="PK812_12780"
     CDS             <1..946
                     /locus_tag="PK812_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007693454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HRJ70469.1"
                     /translation="RLFASPLARRVAKQEGVNLAAVKGSGPHGRIVLADVQGAKKGGV
                     AAAPVAVSAVAPAAAPAPAAAPAGMSGDMVRKMFAPGSYEEIPHDNMRKTIARRLQES
                     KSTIPHFYLTVDCELDALLMLREQINASAPGAKDGKPAFKVSVNDFIIKALALALQRV
                     PDANVSWTDTAMLKHKSSDVGVAVSIPGGLITPVVRNAETKTLSVISNEMKDYAARAK
                     ARKLKPEEYQGGTTAVSNLGMFGIKDFCAVINPPHATILAVGAGEQRAVVKGGAVVPA
                     TVMSVTLSTDHRCVDGALGAELLGAFRQLIENPMGMLV"
     gene            1121..1510
                     /locus_tag="PK812_12785"
     CDS             1121..1510
                     /locus_tag="PK812_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HRJ70470.1"
                     /translation="MAWNRPPEYHVSPRAKLQAESLRRAMTEPERKLWAALRRRLSVS
                     GTHFRRQTPIGPYVADFCCYASRLIVEVDGWQHGLEGQKRHDEVRTRYLEAQGFRVIR
                     FWNHEIIQEIDVVVDTILARIHEQAPL"
     gene            1678..2037
                     /locus_tag="PK812_12790"
     CDS             1678..2037
                     /locus_tag="PK812_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70471.1"
                     /translation="MSFLKALVFYVIFIFGSLIGQRVGGYERDFVVMLMVALALVPAI
                     FLHVLNRALARGIANVLTSVLVMTCLILAAHFGLAAYLKQIPIAVSPAYAVAGIVASF
                     FYQFTARPRARPLGMPG"
     gene            2101..>3488
                     /gene="lpdA"
                     /locus_tag="PK812_12795"
     CDS             2101..>3488
                     /gene="lpdA"
                     /locus_tag="PK812_12795"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006470531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRJ70472.1"
                     /translation="MSNAYDVLIIGSGPGGYVTAIRSAQLGFKTAIVEREHLGGICLN
                     WGCIPTKALLRSAEILHYAQHAKNYGLKLEGSITPDVAEVVKRSRGVSARLNGGVGFL
                     MKKNKIDVIWGEAKLDAPGKVVVGASSKPAMQPQHPAPKGTLGAGTYTAKHIIVATGA
                     RPRALPGIEPDGKLIWTYFEAMVPPAMPKSLIVMGSGAIGIEFASFYRTMGADVTVVE
                     VMPTIMPVEDVEISAIARKAMEKQGMKIHTSTKVTKVVKGANSVTATIADDKGKTQEI
                     TAERMISAVGVVGNIENLGLEKLGVKTDRGCIVIDGYGRTNVPGLYAIGDVAGPPMLA
                     HKAEHEGVICVEKIAGLPVHAMDKGKIPGCTYCHPQVASVGLTEAKAKEAGHAVKVGR
                     FSFAANGKAIALGEDNGLIKTVFDAKTGQLLGAHMVGAEVTELIQGFVVAMGMETTEE
                     DLMHTVFPHPTLS"
BASE COUNT          634 a         1128 c         1126 g          600 t
ORIGIN      
        1 gcgccttttc gcctctccgc tcgcgcgccg cgtggcgaaa caggagggag tcaatctcgc
       61 cgccgtgaag ggttccggcc cgcatggccg catcgtgctg gcggatgtcc agggggcgaa
      121 gaagggcggc gtcgctgctg ctccggttgc tgtttccgca gttgcgccgg ctgccgcgcc
      181 ggctccggct gccgcgcccg ccggaatgtc cggcgacatg gtgcgcaaga tgttcgcgcc
      241 gggttcctac gaggaaattc cgcacgacaa catgcgcaag accatcgcgc ggcgcttgca
      301 ggagtcgaaa tcgacgatcc cgcactttta cctcaccgtg gattgcgaac tcgatgcgct
      361 gttgatgctg cgcgagcaga tcaacgcctc ggcgcccggc gcgaaggacg gcaagccggc
      421 cttcaaggtc tcggtcaacg atttcatcat caaggcgctg gcgctggccc tgcaacgcgt
      481 gccggatgcc aacgtttcct ggaccgacac ggcgatgctg aagcacaagt cttcggatgt
      541 gggcgtggcg gtatcgatcc ccggcgggct gatcacgccg gtcgtccgca acgcggaaac
      601 gaagacgctc tccgtcatct ccaacgagat gaaggactac gcggcccggg cgaaggcccg
      661 gaagctgaag ccggaggaat atcagggcgg caccacggcg gtgtccaacc tcggcatgtt
      721 cggcatcaag gatttctgcg cggtcatcaa tccaccgcat gcgacgatcc tcgccgtggg
      781 cgcgggcgag caacgcgccg tggtgaaggg tggggccgtg gttccggcga ccgtgatgtc
      841 ggtgacgctc tcgaccgacc atcgttgcgt cgacggcgcg ctcggcgcgg aactgctcgg
      901 cgcgttcagg cagctgatcg agaacccgat ggggatgctg gtctagcaat gtcggccgat
      961 ccgggtgatc tcccttcccc cttgcgggga agggccgggg atgggggtgg cgcggcttgg
     1021 ctctgggcgt cagagcggtg aacgcggcgc gcgcggaacg acccccaccc ggcgcttcgc
     1081 gccaccctcc ccgcaagggg gagggaggag tacaggtctg atggcttgga atcgaccgcc
     1141 cgaatatcat gtttcgccgc gcgcaaagct gcaggcggaa tcgctgcgcc gcgcgatgac
     1201 cgaacccgaa cggaaactct gggcggcgct tcggagacga ttgtctgtat cgggtacgca
     1261 cttccgccgg cagacgccta tagggcctta cgttgcggac ttctgttgct atgccagccg
     1321 cctgatcgtc gaagtcgacg ggtggcaaca cggacttgaa ggacaaaagc ggcacgacga
     1381 ggtccgcacc cggtatctgg aagcgcaggg ctttcgcgtc atccgtttct ggaaccacga
     1441 gattattcag gagatcgatg tcgttgtgga cacaattctt gcccgcatcc acgagcaggc
     1501 gccgctatag acggtgtcgc acgccaatga tcgagttgaa gcgacaatgt ccccaatcaa
     1561 cggaacggca agagctatcg ggcaaggctt ccgtcaccga aactgcggcc ttgcgacggg
     1621 gcggaaccga caaccgctgc gccgccggcc cttacatact ggacaggttc ctagccgatg
     1681 agctttctaa aggccttggt gttctatgtg atcttcatct tcggcagcct gatcggccag
     1741 cgtgtcggcg gctacgagcg cgatttcgtt gtcatgctga tggtcgcgct ggcgctcgtg
     1801 ccggcgattt tccttcatgt tctcaaccgg gccctcgccc gcgggatcgc caacgtgctg
     1861 acgagcgtgc tcgtcatgac ctgcctgatc ctggctgcgc atttcggcct cgccgcgtat
     1921 ctcaagcaaa tccccatcgc tgtttcgccg gcctacgccg tggccggcat cgtcgcctca
     1981 tttttctacc agttcacggc gcgtccgcgc gcccgccctc tcggcatgcc ggggtagggg
     2041 cgatatcgtc tgcacacttg aaaacgcctc cgcacggggc ggaaacgacc ggagcctgac
     2101 atgtccaacg cctacgacgt cctcatcatc ggttccggcc ccggcggcta tgtcaccgcc
     2161 attcgctcgg cccagcttgg cttcaagacg gccatcgtcg agcgcgagca cctcggcggc
     2221 atctgcctca actggggctg catcccgacc aaggcactgc tgcgctcggc tgaaatcctg
     2281 cactatgccc agcatgcaaa gaactacggg ctgaagctgg aaggttcgat cacgccggat
     2341 gtggccgagg tggtgaagcg ctcacgcggc gtttcggcgc gcctcaacgg cggcgtcggc
     2401 ttcctgatga agaagaacaa gatcgacgtg atctggggcg aggcgaagct cgacgccccg
     2461 ggcaaggtcg tggtcggggc ctcttccaag ccggcaatgc agccgcagca tcccgcgccg
     2521 aaaggcacgc tcggggcagg gacctacacg gccaagcaca tcatcgtcgc caccggcgct
     2581 cgcccccgcg cgctgcccgg catcgagccg gacggcaagc tgatctggac ctatttcgag
     2641 gcgatggtgc cgcccgccat gccgaaatcg ctgatcgtca tgggctccgg cgccatcggc
     2701 atcgagttcg cctcgttcta ccgcaccatg ggtgctgacg tgacggtggt cgaggtcatg
     2761 ccgaccatca tgccggtgga ggatgtcgag atttccgcca tcgcccgcaa ggcgatggaa
     2821 aagcagggca tgaaaatcca cacctcgacc aaggtgacca aggtggtgaa gggcgcgaat
     2881 tccgtcaccg ccaccatcgc ggacgacaag ggcaagaccc aggagatcac ggccgagcgc
     2941 atgatttccg ccgttggcgt agtcggcaac atcgagaacc tcggccttga aaagctcggc
     3001 gtgaagacgg atcgcggatg catcgtcatc gacggctacg ggcgcaccaa cgtgccgggc
     3061 ctttacgcca tcggcgatgt cgccggcccg ccgatgctcg cccacaaggc cgagcatgag
     3121 ggcgtgatct gcgtggaaaa gatcgccggc ctccctgtcc acgccatgga caagggcaaa
     3181 atcccgggct gcacctattg ccacccgcag gtcgccagcg tcggcctcac cgaggcgaag
     3241 gcgaaagagg cggggcatgc ggtcaaggtc ggccgcttct ccttcgccgc caacggcaag
     3301 gccatcgcgc tgggcgagga caacgggctg atcaagaccg tgttcgacgc aaagaccggc
     3361 cagctcctcg gcgcgcatat ggtcggcgcg gaggtcaccg aactcatcca gggcttcgtc
     3421 gtcgccatgg gcatggagac gaccgaggaa gacctgatgc acaccgtctt cccgcacccc
     3481 acgctctc
//
LOCUS       DAOMXN010000242         3459 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_15086_length_3459_cov_11.076087, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000242 DAOMXN010000000
VERSION     DAOMXN010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15086_length_3459_cov_11.076087"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1026
                     /locus_tag="PK812_12800"
     CDS             <1..1026
                     /locus_tag="PK812_12800"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HRJ70473.1"
                     /translation="LLLAIALMLAALLALRLLERHAAVAPALSATALAAFMVFDLGLN
                     NGPNESTALPPKSYEVMQQDTRNRTIALLKARLAQVTDPARRDRVELLGVGFEWPNLG
                     MIHGFDHVLGYNPLRLREFVEATGAGDTIAGPDQRTFTPLMPSYRSTLSDLMGLRWIA
                     SSVPVEQVDKTLRPGDLRFVGMTEDGYVYENPRALPRAMFVRNWQVADFERLTETGVW
                     PENFNPRQTVLLEANPEQMNAPRMTGPAQVAITDYDNTRVEITVTSPDAGFVLLNDVW
                     HRWWTVTVDGEEAEILKANVLFRAVQVPAGKHTIVFEFKPIEGAFAELREKLTGEEPD
                     APAVDTP"
     gene            complement(1120..2880)
                     /gene="phaC"
                     /locus_tag="PK812_12805"
     CDS             complement(1120..2880)
                     /gene="phaC"
                     /locus_tag="PK812_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HRJ70474.1"
                     /translation="MPDVEELAENSARIAAEVSKAATAYVKGLESGAVKPAPSESADM
                     LKTFSAVAESWLAHPERTVEVQKQIASDVMGLWAHTFKVMQGEKPEPANSAARDNRFA
                     DPDWEKNPYFHLIKHAYLLGSNWAQNLVDKAEGIDEATKRRAAFYVKQLQAAFAPSNY
                     LLTNPELIKQTIEEKGANLARGMQMLAEDMEKGGGSLRIRQVDDRGFEVGKNMAVTPG
                     KVVFRNELMELIQYAPATETVFKRPLLIVPPWINKFYILDLNPEKSFIKWCVDQGLTV
                     FVVSWVNPDERHRACGFAEYMKLGPVAALDVIEEITGEKDVQAIGYCVGGTLLAVTLA
                     WLAAKGMGARINRATFFTTQVDFTYGGDLLAFVSEDSIKALDEKMAETGYLSGASMAN
                     AFNMLRPQDLIWSYVVNNYMKGKQPMPFDLLYWNGDSTRMPAANHTFYLRNCYLENNL
                     SKGKMVVDGVKLTLKKVKTPIYNLAAKEDHIAPAKSAFVGSQFFGGPVKYVMAGSGHI
                     AGVVNPATKPKYQYWTGDEPKGAFEDWVKTAKENPGSWWPDWLAWCTKGDKQVRARKP
                     GSRKHKPLCDAPGTYVKVRV"
     gene            3075..>3459
                     /locus_tag="PK812_12810"
     CDS             3075..>3459
                     /locus_tag="PK812_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70475.1"
                     /translation="MRARRLTTSLNAALLSAGLLGLGGCALTPFDEEGTFRHVTTAAG
                     ATGKLQEPAPFVRETRPAELEYQPVGVTPGRPTEARRGTAVQDLEKELRARQAASVQQ
                     ASRPVPRSPYDGKIEPGFRPPAPAPL"
BASE COUNT          570 a         1162 c         1069 g          658 t
ORIGIN      
        1 cttctgctcg ccatcgcgct gatgctggcg gcgctgctcg cgctgcggct gctggagcgc
       61 catgccgctg ttgctccggc gctgtcggcc accgccctcg ccgccttcat ggtgttcgac
      121 ctcggcctca acaacggccc gaacgaatcc accgccctgc cgccgaagtc ttatgaggtg
      181 atgcagcagg acaccaggaa tcgcaccatc gcattgctca aggcgcgcct ggcgcaggtg
      241 acggatcctg cccgccgcga ccgcgtggaa ctgctcggcg tcggcttcga atggccgaac
      301 ctcggcatga tccacggctt cgatcacgtg cttggctaca acccgctccg cctcagggaa
      361 ttcgtcgagg ccacgggcgc gggcgacacg atcgcggggc cggaccagcg caccttcacg
      421 ccgctgatgc cctcctatcg ctccacgctg tcggacctga tgggcctgcg ctggatcgcc
      481 tccagcgtcc cggtcgagca ggtggacaag accctgcgcc ccggcgatct gcgcttcgtc
      541 ggcatgacgg aggacggcta cgtctatgaa aacccccgcg ccttgccccg tgccatgttc
      601 gtgcgcaact ggcaggtcgc cgatttcgag cgcctgaccg agaccggcgt ctggccggag
      661 aactttaacc cacgccagac agtgctgctt gaagccaatc ccgagcagat gaacgcaccg
      721 cgcatgaccg gcccggcgca ggtcgccatc accgactacg acaacacccg cgtcgagatc
      781 accgtgacct cgcccgacgc cggcttcgtc ctgctgaacg atgtctggca tcgctggtgg
      841 accgtgacgg tcgacggcga ggaggccgaa atcctcaagg ccaacgtgct gttccgcgcg
      901 gtgcaggtgc cggccggcaa gcacaccatc gtcttcgagt tcaagccgat cgagggcgcg
      961 ttcgcggagt tgcgcgagaa gctgacgggc gaggagccgg acgcgccggc ggtggacacg
     1021 ccgtaggcgg acctgcggcc gcgcaagaac gaacgcttgc gacgaccgaa gatggctcga
     1081 ccgcaccgat ccccaggcac ggctgcacgc agtgtagcct tacaccctga ccttcacata
     1141 ggtgcccggc gcgtcgcaga gcggcttgtg cttcctgctg ccgggcttgc gggcgcgcac
     1201 ctgcttgtcg cctttcgtgc accaggcgag ccagtccggc caccagctgc ccgggttttc
     1261 cttcgccgtc ttcacccagt cctcgaaggc gcccttgggc tcgtcgccgg tccagtactg
     1321 gtacttcggc ttggtcgccg gattgacgac gccggcgata tgccccgagc cggccatgac
     1381 gtatttcacc ggcccgccga agaactggct gccgacgaac gccgatttcg ccggggcgat
     1441 atggtcttcc ttcgccgcca gattgtagat cggcgtcttc accttcttca gcgtcagctt
     1501 gaccccgtcg accaccatct tgcccttgga gaggttgttc tcgaggtagc agttgcgcag
     1561 gtaaaacgtg tggttggccg ccggcatgcg ggtggaatcg ccgttccagt acaacaggtc
     1621 gaagggcatc ggctgcttgc ccttcatgta gttgttgacg acatagctcc agatcaggtc
     1681 ctgcggccgg agcatgttga aggcattggc catgctggcg ccggacagat agcccgtctc
     1741 tgccatcttt tcgtcgagcg ccttgatgct gtcctcgctg acgaaggcaa gcaggtcgcc
     1801 gccgtaggtg aagtcgacct gcgtggtgaa gaacgtcgcg cggttgatgc gcgcgcccat
     1861 gcccttcgcc gcgagccagg ccagcgtcac cgccagcagc gtgccgccga cgcaatagcc
     1921 gatggcctgc acatcctttt cgcccgtgat ctcttcgatc acatccagcg ccgcgaccgg
     1981 tcccagcttc atgtactcgg cgaagccgca agcgcggtgg cgctcgtcgg gattgaccca
     2041 ggagacgacg aacaccgtca gtccctggtc cacgcaccac ttgatgaagg acttttccgg
     2101 gttcaggtcg aggatataga atttgttgat ccacggcggc acgatcagca gcggccgctt
     2161 gaacaccgtc tcggtggccg gtgcgtactg gatcagttcc atcagctcgt tgcggaacac
     2221 gaccttaccg ggcgtgaccg ccatgttctt gccgacctcg aagccgcggt cgtccacctg
     2281 gcggatgcgc agcgaaccgc ctcccttctc catgtcctcc gcgagcatct gcatgcctcg
     2341 ggcgaggttg gcaccctttt cctcgatcgt ctgcttgatc agttccggat tggtcagcag
     2401 gtagttcgac ggcgcgaagg cggcctgaag ctgttttaca tagaaggcgg cgcggcgttt
     2461 tgtcgcctca tcgatgcctt ccgccttgtc gacgaggttc tgcgcccagt tcgatccgag
     2521 caggtaggca tgcttgatca ggtgaaaata ggggttcttt tcccagtcgg gatcggcgaa
     2581 acgattatcg cgcgcggccg agttggcggg ttccggcttc tcgccctgca tcaccttgaa
     2641 cgtgtgcgcc cagaggccca tgacatcgct ggcgatctgc ttttgcacct ccaccgtgcg
     2701 ctcgggatgg gcaagccagc tctcggcgac ggccgagaag gtcttcagca tgtcggcgga
     2761 ttcggacggc gccggcttta cagcgccgct ttcaagtccc ttgacatagg ccgtcgccgc
     2821 cttcgaaacc tcggccgcga tccgggcaga attttccgcg agttcctcga catccggcat
     2881 ctggtttgaa aacggatcgg attccggctt gccctcgacg ctcgacacga cctgctccct
     2941 atgacctttg gtctatatcc gcccgtcacg ttggggccat gttctgacgg aacaatgccc
     3001 ttcagatgaa cacgtcctaa cggacagtca accatactgt gtgacgatct ggaccataca
     3061 ggattttcgg gacaatgcgt gcgcgccgcc taactacttc gcttaatgcc gccctgcttt
     3121 ccgccggcct tctcggcctt ggcggttgtg cgctgacgcc gttcgacgaa gaggggacat
     3181 tccggcacgt cacgacagcg gcgggcgcca ccgggaaact gcaggagccc gcgcctttcg
     3241 tccgcgaaac gcggccggcg gaactggaat accagccggt cggggttacg ccgggccggc
     3301 cgaccgaggc gcgtcgcggc actgcggtgc aggacctgga aaaggagttg cgcgcgcggc
     3361 aggccgccag cgttcagcag gccagtcgac ccgtgccgcg ttcgccctac gacggcaaga
     3421 tcgaacccgg cttccggccg ccggcgccag cgcctctgc
//
LOCUS       DAOMXN010000243         3455 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_15116_length_3455_cov_9.540000, whole genome shotgun sequence.
ACCESSION   DAOMXN010000243 DAOMXN010000000
VERSION     DAOMXN010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15116_length_3455_cov_9.540000"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /locus_tag="PK812_12815"
     CDS             <1..998
                     /locus_tag="PK812_12815"
                     /inference="COORDINATES: protein motif:HMM:NF021606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HRJ70476.1"
                     /translation="ALRWAVAALGAVIAARLAWDPRIVGANLGTTPVFNWLLVGYGIP
                     ALSFAGCAWLLRREPEDLPVQIAQALALVFAALLCFYEIRHALNGGDPFARTTTLTED
                     ALHACMAFAFAIGTTLMTTARRSVVFSSASLAFGVAAIALTGFLLLFADNPYLTRSRI
                     EGGTLFNTLILAYALPALLATTLARLSIGRRPEWYRIMAAVAAIALAFAYVNLQTRAF
                     FQGPSRLHHKLGMLDSEFYAYSAVWLALGLALLGYGIVRKSREARFGSALLVFAATFK
                     IFLFDLARLEGALRALSFIGLGLVLIGIGLVYQKLLFRSKPAASTAPATPPEAGG"
     gene            complement(1030..1593)
                     /locus_tag="PK812_12820"
     CDS             complement(1030..1593)
                     /locus_tag="PK812_12820"
                     /inference="COORDINATES: protein motif:HMM:NF022115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2478 domain-containing protein"
                     /protein_id="HRJ70477.1"
                     /translation="MTASIGAIVYPEGIYPDRMMTEIAHTLSAAGTRLAGVVQHNTCP
                     PGRSRCDMLLEDLRTGAIIRISEDRGEQSRGCRINPQGLAEASASVAAALAGDLPELL
                     IINKFGKMEAEGDGLRDLIAQAIGQDVPVLIGVPQRNLDAWRAFAGSFADELPPMQHA
                     VRAWLQGRGLLPLEEARSARLRAPALV"
     gene            complement(1605..2141)
                     /gene="mog"
                     /locus_tag="PK812_12825"
     CDS             complement(1605..2141)
                     /gene="mog"
                     /locus_tag="PK812_12825"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HRJ70478.1"
                     /translation="MSQETARIGIVTVSDRASAGIYADDSGPAIIAYLKELLVSPWEA
                     VARVIPDGVASVSAALIDLADVEGCDLVFTTGGTGPAPRDQTPEGTRAVIERELHGFG
                     ELMRKISFEKVPTAILSRQLAGTRGKTLIVNLPGKPASIRTCMAAVFPAIPYCLDLMG
                     ARRLETDPKVCVAFRPPA"
     assembly_gap    2289..2388
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2389..3071)
                     /locus_tag="PK812_12830"
     CDS             complement(<2389..3071)
                     /locus_tag="PK812_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HRJ70479.1"
                     /translation="MVLDDLFDRNVAWALRKTRDDPGFFRRLAEQQSPRYLWIGCSDS
                     RVPANDIVGLDPGEIFVHRNIANVVHTSDMNLLSVLEFAIEHLKVEHIIVCGHYGCGG
                     VRRALEGATGALVDHWLAPVIDLYASYKDELDSIEDTLARVDRLCELSVELQARRLGA
                     NPIIHRAWRAGQKLKLHGWIYGLHDGMLRDLGITLASEAEFRSLGSMDDSVRRQQEPL
                     TALRQHAIEA"
     gene            complement(3085..>3455)
                     /locus_tag="PK812_12835"
     CDS             complement(3085..>3455)
                     /locus_tag="PK812_12835"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HRJ70480.1"
                     /translation="IADFLRRDMYLHHQDEELDLFPALRRRAHQEDNLAGVLDRLADD
                     HHASEPNAVEIARALEATPQEGLVSVSERIQRRMLALAASEHRHLAIENGLVMAIARV
                     RLTRSDLARMGDAMRARRAT"
BASE COUNT          539 a         1135 c         1101 g          580 t
ORIGIN      
        1 cggcgctgcg ctgggccgtc gccgcactcg gcgcagtgat tgcggcgcgg cttgcctggg
       61 acccgcgcat cgtcggcgcc aatctcggaa cgacaccggt cttcaactgg ctgctcgtcg
      121 gttacggcat cccggcgctg agtttcgccg gctgcgcctg gctgttgcgg cgcgagccgg
      181 aggacctgcc ggtacagatc gcccaggcgc tggccctcgt cttcgcggcg ctgctctgtt
      241 tctacgagat ccggcacgcg ctgaacggag gcgatccctt cgcccgcacg acgacgttga
      301 ccgaggatgc cctgcacgcc tgcatggcct tcgccttcgc gatcggcacg acgctgatga
      361 cgacggcgcg ccgaagcgtc gtcttttcca gcgcgtcact ggccttcgga gtggcggcca
      421 tcgcgctaac cggattcctg ctgctgttcg cagacaatcc ctacctgacg cgcagccgca
      481 tcgagggcgg cacgcttttc aacacactga tcctcgccta tgccctgccg gcgctgctgg
      541 cgacgacgct cgcccggctc agcatcggcc gccggcccga atggtaccgg atcatggcgg
      601 cggtcgcggc catcgcgctg gccttcgcct atgtgaacct gcagacccgc gccttcttcc
      661 agggcccgtc gcgcctccac cacaagctcg gcatgctcga cagcgagttc tatgcctact
      721 ctgcggtctg gctggcgctc ggcctggcgc tcttgggcta cggcatcgtg cggaagtcgc
      781 gcgaggcgcg cttcggctcg gcgcttttgg tcttcgccgc cacgttcaag atcttcctgt
      841 tcgatcttgc gcggctggag ggcgcgttgc gcgcgctgtc cttcatcggc ctcggactgg
      901 tgctgatcgg gatcgggctc gtctaccaga agctgttgtt ccggtcgaag ccggccgcgt
      961 ccaccgcgcc ggccacgcca ccggaggcag gcggctgacg tcgttgtccg gcttcaggtc
     1021 aagaccgcgt cagacgagcg caggcgccct gagccgcgcg gagcgggcct cctccagcgg
     1081 caggaggccc ctgccctgca accaggcgcg caccgcatgc tgcatgggcg gcagttcatc
     1141 cgcaaacgat ccggcgaagg cgcgccaggc gtcgaggttg cgttgcggca cgccaatcag
     1201 caccggaaca tcctggccga tggcctgagc gatcaggtcg cgcaggccgt cgccttccgc
     1261 ttccatcttg ccgaacttgt tgatgatcaa cagctccggg aggtcgccag ccaacgccgc
     1321 ggcgaccgag gccgaagctt cggccagccc ctgcgggttg atgcggcagc cgcgcgactg
     1381 ctcgccccga tcctcggaaa tccggatgat cgcgccggtg cgcaggtctt ccagcagcat
     1441 gtcgcaacgc gaccggccgg gagggcaggt gttgtgctgc acgacgcccg ccagccgcgt
     1501 cccggcggca ctcaacgtgt gggcgatctc ggtcatcatc cggtccggat agatgccctc
     1561 gggatagacg atggctccga tgctcgcggt catggcaaac tccttcaggc agggggacgg
     1621 aacgcgacgc agacctttgg atcggtctcc agacggcgcg cgcccatcag gtcgaggcaa
     1681 taggggatgg cggggaaaac cgccgccatg caggtgcgga tcgaggcggg cttgcccggc
     1741 aggttgacga tcagtgtctt gccgcgggtt ccggccaact ggcgggagag gatggcggtg
     1801 gggacctttt cgaagctgat cttgcgcatc agctcgccaa acccgtgcag ttcccgctcg
     1861 atcacggcgc gggtgccctc cggcgtctgg tcgcgcggcg ccgggccggt cccccccgtg
     1921 gtgaagacca ggtcgcagcc ttccacatcg gccagatcga tcagagcggc ggaaacggac
     1981 gccaccccgt ccgggatgac gcgtgccacc gcttcccagg ggctgaccaa aagttccttc
     2041 aggtaggcaa tgatcgcggg gccgctgtca tccgcataga tgccggcact cgcgcggtcc
     2101 gaaacggtga cgatgccaat gcgggcggtt tcctgtgaca tgaggcggtc aaactccgag
     2161 catccgaacg ggcccatgat cgtcgcaaca caggagccct tcctcgggtt ccagcgacgc
     2221 caattgcgaa aaggcttcaa tcgcgtgctg gcgcaaggcc gtcaatggct cctgctgacg
     2281 acggacacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc ttcaatcgcg
     2401 tgctggcgca aggccgtcaa tggctcctgc tgacgacgga cactgtcatc catggaccca
     2461 agagaacgga attccgcctc cgaggccagg gttatgccga ggtcgcggag cattccgtca
     2521 tgcaggccgt agatccagcc gtgcagcttg agcttctggc cggcgcgcca ggcccggtga
     2581 atgatcgggt tggcgccgag acggcgcgcc tgcaactcga cgctcagttc gcagagccgg
     2641 tcgacgcggg cgagggtatc ctcgatgctg tccagttcat ccttgtagct tgcatagagg
     2701 tcgatcaccg gcgccagcca atgatccaca agggcacccg tcgccccttc cagcgcccgt
     2761 cgcacgccgc cacagccgta atggccgcag acgatgatat gctcgacctt cagatgctcg
     2821 atcgcgaatt ccaggaccga caacaggttc atgtcgctgg tgtggacgac gttggcgatg
     2881 ttgcgatgca cgaagatctc gccgggatcg agcccaacga tgtcgttcgc cggaacccgg
     2941 ctgtcggagc agccgatcca gaggtaacgt ggcgattgct gctcggcgag acggcggaag
     3001 aagccgggat catcccgggt cttgcgcagg gcccaggcca cgttgcgatc aaacaggtca
     3061 tcgagaacca aggctcagtc cctttcaagt cgcgcggcgc gcgcgcatcg cgtcgcccat
     3121 ccgcgcaagg tcagatcgtg tcagccggac gcgcgcgatc gccatgacca gcccattctc
     3181 gatggcgaga tgacggtgct cgctcgccgc gagggcgagc atgcgccgct ggatccgctc
     3241 ggacaccgac accagcccct cttgcggggt ggcttcgagc gcacgggcga tctccaccgc
     3301 gttcggttca ctggcgtggt gatcgtccgc cagcctgtcg agcacgcccg ccagattgtc
     3361 ctcctgatgc gcgcgccgcc gcaacgccgg aaagagatcg agttcctcgt cctgatgatg
     3421 caggtacatg tcgcggcgca ggaaatcggc gatct
//
LOCUS       DAOMXN010000244         3453 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_15128_length_3453_cov_12.015892, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000244 DAOMXN010000000
VERSION     DAOMXN010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15128_length_3453_cov_12.015892"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1135)
                     /gene="ftsZ"
                     /locus_tag="PK812_12840"
     CDS             complement(<1..1135)
                     /gene="ftsZ"
                     /locus_tag="PK812_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017486201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRJ70481.1"
                     /translation="MTINLQAPDIRELKPRITVFGCGGAGGNAVNNMIECGLVGVDFV
                     VANTDAQALRLSRADRRVQMGVQVTEGLGAGSMPEVGRSAAEEVLDEIRDQIAGAHMV
                     FITAGMGGGTGTGAAPVVARAARELGILTVGVVTKPFHFEGPRRMAVAEAGITELQKS
                     VDTLIIIPNQNLFRVANERTTFAEAFAMADQVLYSGVASITDLIVKPGLINLDFADVR
                     AVMREMGKAMMGTGESTGENRAIQAAEAAISNPLLDEVSMKGARGLLISITGGRDMKI
                     YEVDEAANRIRQEVEADANVIFGATQDESLDGIIRVSVVATGLDRTAAAQPQISADDR
                     RLHEMAEKLRAHMAARAQSQVAAPAPTQPSSRSSTAASQPSARS"
     gene            complement(1268..2587)
                     /gene="ftsA"
                     /locus_tag="PK812_12845"
     CDS             complement(1268..2587)
                     /gene="ftsA"
                     /locus_tag="PK812_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010685846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HRJ70482.1"
                     /translation="MSSRGSMSPRIKPVSAKKSVILSALDVGTSKVACLIARLDPMES
                     SDLLRGRTHRMRILGIGHQRSRGLKGGVVIDLEEAERAVRLAVDAAERMAGVQVESTI
                     VNITGGRLASRHFTGSVNLHRRTITDYEVHRVMDAAASQSATAGRTVLHSLPTGYCLD
                     ESRHIRDPRGMIGDHLHVDMHVATCDTLAQRNLMIAVERGHLKVEAMVATPYASGLSA
                     LVDDEAEMGAACIDLGGGTTSVGIFSHGRLVHVDAVAVGGNHITMDLARGLSMRLEDA
                     ERLKNLSCSCIESASDDRDLIAIRQVGEDARDYPNQIPRSQVTRIVKPRVEEILELVR
                     DRLNRAGVGLNAGQRVVLTGGGAQLSGIADQARRILTKQVRVGRPVGVQGLPEAAKSS
                     AFATAVGLLVYPQVAALEHFDAPRRRGGATGTDGYFSRVGRWLRDSF"
     gene            complement(2587..>3453)
                     /locus_tag="PK812_12850"
     CDS             complement(2587..>3453)
                     /locus_tag="PK812_12850"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HRJ70483.1"
                     /translation="ERRARGHRLPRAAARRAAARRFDLPRGLGSFVVICFLSAITATG
                     IVAGGHYDRFKAEYGSVRDIAARMLGMRVASVAVIGNRELSVEEVFLGAAIPATASLP
                     FLDIGSVQNRLKSVPLIADASVRKLYPDKLAIQITERSPFALWQLDGQVKLVSADGTA
                     IDVLRDDRFVMLPHVVGPGANKRVKEYVGILLEVPELADQVRAGTLVGERRWTLKLKN
                     GVDVKLPEDEPARALARLAEVDRDSRLLSKDIIGVDLRQPDRIVVRLSEEAAQARNEL
                     FDKKIDKLKRRS"
BASE COUNT          593 a         1170 c         1083 g          607 t
ORIGIN      
        1 ccgagcgtgc cgagggctgc gacgccgccg tactgctcct cgacgacggc tgcgtaggcg
       61 cgggcgccgc gacctggctc tgggcgcgtg ccgccatgtg cgcgcgcagc ttttcagcca
      121 tttcgtgcag gcggcgatcg tccgccgaga tctgcggctg tgcggccgcc gtgcggtcaa
      181 gaccagtggc gaccacagac acccgaatga tgccatcgag gctctcatcc tgtgtcgcgc
      241 cgaagatgac gttggcgtcc gcctcgactt cctggcgaat ccggttggcc gcctcgtcga
      301 cttcgtagat cttcatgtcg cggccgccgg taatggagat caagagaccc cgggcgccct
      361 tcatcgagac ttcgtcgagc agcggattgg agatcgccgc ttccgccgcc tgaatggcgc
      421 ggttctcgcc ggtcgattcg ccagtgccca tcatcgcctt gcccatctcg cgcatcaccg
      481 cgcgaacgtc ggcgaagtcg aggttgatca ggcccggctt gacgatcagg tcggtgatcg
      541 acgcgacgcc ggagtaaagc acctgatcag ccatggcgaa ggcctcggcg aacgtggtgc
      601 gctcgttggc cacgcggaac aggttctggt tcggaatgat gatgagcgtg tcaacgctct
      661 tctgcaattc ggtgatcccg gcttccgcca cggccatgcg gcgcggcccc tcgaaatgga
      721 acggcttggt gacaacgcca acggtcagga ttcccagttc gcgcgcggcg cgggccacga
      781 cgggagcggc tcccgtgccg gtgccgccgc ccatgccggc ggtgatgaac accatgtgtg
      841 cgccggcgat ctggtcgcgg atctcgtcga gaacctcttc cgcggccgag cggccgactt
      901 ccggcataga accggcgccg aggccttcgg tcacctgcac gcccatctgg acgcggcgat
      961 cggcgcgcga cagcctgagc gcctgcgcat cggtattggc gacaacgaag tcgacgccca
     1021 ccagaccgca ttcgatcatg ttgttgacgg cattgccgcc tgccccgccg caaccgaaca
     1081 cagtgatccg cggcttgagt tcccggatgt ccggagcctg caggttgatg gtcatttcaa
     1141 gcctctttcc gttgggagcg tccgccgcga cgccgccgtt accactctac gctcagtcga
     1201 accgtaaacc gttaaggtta actcattgta atcgcggatg ggtttctgcc tgtcccattc
     1261 ctgaatttca gaaactgtcg cgcagccagc gtccgacacg gctaaaatag ccgtccgtcc
     1321 ccgttgcccc gccgcgccgc cggggcgcgt cgaaatgctc cagtgccgcc acctgcggat
     1381 agaccaacag ccccacggct gtcgcgaaag cggagctttt cgcggcttcc ggcagacctt
     1441 gcacgcccac cggacgcccg acacggacct gtttcgtcag gatcctgcgc gcctgatcgg
     1501 caatcccgga gagctgggct cctccgcctg tcagcaccac gcgctggccc gcattgagcc
     1561 cgacgccggc gcgattgagc cggtcgcgca ccagctcgag gatttcctcg acacgcggct
     1621 tgacgattcg tgtcacctgg gagcgcggaa tctggttggg atagtcacgc gcatcctcgc
     1681 caacctgccg gatggcaatc aggtcccggt cgtccgaggc gctttcgatg caggagcagg
     1741 agagattctt caaccgctcg gcatcctcaa ggcgcattga cagcccgcgc gccaggtcca
     1801 tcgtaatgtg attgccgcca accgccaccg cgtcgacgtg cacgagacgg ccatgactga
     1861 aaatgccgac actcgtggtg ccaccgccca ggtcgatgca ggccgcgccc atctccgctt
     1921 cgtcatcgac cagagctgaa aggcccgagg catagggcgt cgccaccatc gcctcgacct
     1981 tcagatggcc gcgttccacc gcaatcatca ggttgcgctg cgcgagcgtg tcgcaagtcg
     2041 ctacatgcat gtcgacatgc aggtgatcgc cgatcatgcc gcggggatcg cggatatggc
     2101 gggactcgtc caggcaatag cccgtaggca gcgagtgcag caccgtccga ccggccgtcg
     2161 cgctctgcga cgcggcggca tccatcacgc ggtgcacttc atagtccgtg atggtccgcc
     2221 gatgcagatt gaccgaaccg gtgaaatgcc gcgaggccag acgcccgccg gtaatgttga
     2281 cgatggtcga ttcgacctgc acgcccgcca tccgctcggc cgcgtccacc gccagccgga
     2341 cagccctctc ggcttcttcc agatcgatga caacgccacc cttgagcccg cgcgagcgct
     2401 ggtgaccgat gcccaggatg cgcatgcggt gcgtgcggcc acgcagcagg tcgctggact
     2461 ccatcggatc gagccgggcg atcaggcagg cgaccttgct cgttccgacg tcgagcgcgg
     2521 acaggataac gctcttcttg gccgaaaccg gcttgatcct tggagacatg ctaccacgcg
     2581 aggacatcaa ctccgcctct tcagcttgtc gatcttcttg tcgaacagtt cattgcgcgc
     2641 ctgtgccgcc tcttcgctca gcctgacgac gattctgtcg ggctggcgca ggtccacgcc
     2701 gatgatgtcc ttcgacaaca ggcgggaatc ccggtcgact tcggcaaggc gcgccaacgc
     2761 gcgcgccggt tcgtcctcgg gcagcttcac atcgacgccg ttcttcagct tcaaggtcca
     2821 gcgccgctcc cctacaaggg ttccggcgcg cacctggtcc gccagctccg ggacttccag
     2881 aagaatgccg acatattcct tgacgcgctt gttggcgccc ggcccgacga catggggcag
     2941 catgacgaaa cggtcatcac gcagcacgtc gatggcggtg ccgtcggccg ataccagctt
     3001 gacctgtcca tccagttgcc agagcgcgaa gggtgagcgc tcggtgatct ggatcgccag
     3061 cttgtccgga taaagcttgc gcaccgacgc atcggcgatg agcgggaccg atttcagccg
     3121 gttctggacg gaaccgatgt cgaggaaggg cagcgaggcc gtggccggga ttgccgcgcc
     3181 aaggaagact tcctcgaccg acagttccct gttgccgatc acggcgacgg acgcgacccg
     3241 catcccgagc atgcgcgccg cgatgtcgcg aacgctgccg tactccgcct tgaagcgatc
     3301 ataatgaccg cccgcgacaa ttccggtcgc cgtgatggcg gacaggaagc agatgacgac
     3361 gaaagacccg aggcctcggg gcagatcgaa gcggcgtgcg gcggctcgac gggcggcagc
     3421 gcgcggaagg cggtggccac gcgctcgtcg ttc
//
LOCUS       DAOMXN010000245         3422 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_15313_length_3422_cov_10.335016, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000245 DAOMXN010000000
VERSION     DAOMXN010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15313_length_3422_cov_10.335016"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..572)
                     /locus_tag="PK812_12855"
     CDS             complement(171..572)
                     /locus_tag="PK812_12855"
                     /inference="COORDINATES: protein motif:HMM:NF014958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HRJ70484.1"
                     /translation="MPHLIIDYSANLDAALDLRALTDGLHAAALETGVFPVGGCRTRL
                     ARRETFAVGDGHPGNAYIHVQARIGAGRPPEVRQKAAEHIFERLKALTAERFARAPLA
                     LSFEIVEMDPVGSLKHNNLHEIIEQRRKGTP"
     gene            complement(624..1082)
                     /gene="hpaR"
                     /locus_tag="PK812_12860"
     CDS             complement(624..1082)
                     /gene="hpaR"
                     /locus_tag="PK812_12860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HRJ70485.1"
                     /translation="MRPLEHSLPLKLLNAREAVMERFRPHLHANGLTEQQWRVLRALA
                     EAEELDVGTLSRRITLLMPSLSRMLPDLEKRGLLSRRRSPQDARLMLARLTGEGQALF
                     ARVGAESERIYAAIEAQVGRQTLQELMRALDTVQARLAAPRDPAGGGSGA"
     gene            complement(1079..1951)
                     /locus_tag="PK812_12865"
     CDS             complement(1079..1951)
                     /locus_tag="PK812_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007757693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRJ70486.1"
                     /translation="MRLARWQNENGDAFGCVTADKKLVDLTGVFGWQIPDVKSLIGAE
                     LPEADLARKEKGRLGAAMVGTRWLPPVENPGKIICVGVNYPERNAEYKDGSDQPKFPS
                     LFVRFPSTFVGHEQPILRPRESEQFDYEGEIVLVIGKPGRRIAEADALEHVFGVTLMN
                     DGSVRDWIRHGKFNVTQGKNFEKSGSIGPWIVTREAAGDLNDLTVITRVNGEERQRGN
                     TGTLLFPIARIIAYVSTFITLEPGDLIATGTPPGSGARFEPPKWLKPGDVVEVECPQI
                     GVLRNTVADEPAGA"
     gene            complement(2053..2688)
                     /gene="maiA"
                     /locus_tag="PK812_12870"
     CDS             complement(2053..2688)
                     /gene="maiA"
                     /locus_tag="PK812_12870"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HRJ70487.1"
                     /translation="MKLFDYFRSGAAYRVRIALNLKGIPVDHASVHLRRGEQRGEAYL
                     RLNPQGLVPALVTDGGDVLTQSLAIIHWLEARYPEPSLLPQEPLQRARVEAFVYAIVC
                     DTHPVQNLKVLNKLRAMGHSEDEVTGWAAWVNAEGLGACEKLVAAQPGPFCFGDQPSL
                     ADVCLVPQLGNARRFKVDVSAFPRLLQAEAAAKAHPAFQAAAPEQQADFEA"
     gene            2906..>3422
                     /locus_tag="PK812_12875"
     CDS             2906..>3422
                     /locus_tag="PK812_12875"
                     /inference="COORDINATES: protein motif:HMM:NF020897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRJ70488.1"
                     /translation="MVALAMGAGATTDRMRSISVHTFTSAASARGIGFARAGGRERPG
                     TQAVDRALWLLLHMAGETGAGLSLKDVSASAGLDPATTHRLLGSLKRFGLVEQNPQTR
                     RYGLGLEFFALAAAASGRLQLAERIRPTLAALSRASGLRSVCFVRNGPDLVCVETHPG
                     RSPSAFGARDRG"
BASE COUNT          515 a         1176 c         1151 g          580 t
ORIGIN      
        1 cacggcggca atgaccgatg tgtcgaccgg cgaggccgtc tcgaacgtcc cggcgctctc
       61 gacgcgcgcg ccgccgatga catggccgat gccatcgcgg ccgaggcgct ccagaagcgg
      121 cttgagccgc gcctcgttct ccgcgaaacc cggtcccttg gccctggcgc tcatggcgtg
      181 cccttgcgtc gctgttcgat gatctcgtgc aggttgttgt gcttgaggct gccgaccggg
      241 tccatctcga cgatctcgaa gctcagggcc agcggcgcgc gggcgaaccg ctctgccgtc
      301 agcgccttca accgctcgaa gatgtgttcg gccgccttct gccgcacctc aggcgggcgc
      361 cccgcgccaa tgcgggcctg cacatggatg taggcgttgc ccggatggcc atcgccgacc
      421 gcgaaagtct cccgtcgcgc caggcgcgtg cggcagcctc cgacggggaa gacgcctgtc
      481 tccagcgccg ccgcatggag gccgtcggtc agcgctcgca ggtccagcgc cgcatcgaga
      541 ttggcggaat agtcgatgat cagatgcggc accctcgcgc cctccgacaa ttcattatca
      601 tgttaaataa taagcctgcg ctgtcaagcg ccggaaccac cgccggcggg gtcccgtggc
      661 gccgcgagcc gggcctgcac cgtgtccagc gcccgcatca gctcttgcag cgtctgacgg
      721 ccgacctgtg cctcgatcgc ggcatagatc cgctcgctct cggcgccgac ccgggcaaac
      781 agcgcctggc cctcgccggt cagcctcgcc agcatcagcc gggcgtcctg cggcgagcgg
      841 cggcggctga gcaggccgcg cttctcgaga tcgggcagca tgcgcgagag gctcggcatg
      901 agcagcgtga tgcgccgcga cagcgtgccg acatcgagtt cctcggcctc ggccagcgcg
      961 cgcagcacgc gccactgctg ttcggtcagt ccgttcgcgt gcaggtgggg acggaaccgc
     1021 tccatcaccg cctcgcgtgc attgagcagc ttcagcggca gcgagtgctc cagcggcctc
     1081 atgcgccggc cggctcgtcc gcgaccgtgt tgcgcaggac gccgatctgc gggcactcga
     1141 cctcgaccac atcgcccggc ttcagccact tcggcggctc gaaccgcgcg cccgatccgg
     1201 gcggcgtgcc ggtggcgatc aggtcgccgg gctcaagcgt gatgaaggtc gagacatagg
     1261 cgatgatgcg ggcgatcggg aacagcagcg tcccggtgtt gccgcgctgg cgctcctcgc
     1321 cgttgacgcg cgtgatcacc gtcaggtcgt tgaggtcgcc tgcggcctcc cgcgtcacga
     1381 tccagggacc gatggagccg gatttctcga aattcttgcc ctgggtgacg ttgaacttgc
     1441 cgtgtcggat ccagtcgcgc acggagccgt cgttcatcag ggtcacgccg aagacatgtt
     1501 caagcgcatc cgcctcggcg atgcgacggc ccggcttgcc gatcaccagg acgatctcgc
     1561 cctcgtagtc gaactgctcg gattcgcgcg ggcgcaggat cggctgttcg tgcccgacga
     1621 acgtggacgg gaagcggacg aacaggctcg ggaatttcgg ttggtccgag ccgtccttgt
     1681 attccgcgtt gcgctccggg tagttcacgc cgacgcagat gatcttgccc gggttctcga
     1741 ccggcggcag ccagcgtgtc cccaccatcg cggcgccgag ccgccccttc tccttgcggg
     1801 ccagatccgc ttccggcaac tccgctccga tcagcgactt cacatccggg atctgccagc
     1861 cgaagacgcc ggtcaggtct accagcttct tgtcggcggt cacgcacccg aaggcatcgc
     1921 cattttcatt ctgccagcgc gcaagacgca tggaacgctc cacaagccgc cggtctcgtt
     1981 ccggcgcgcc tcggtatcac aacccggaag cacggagcaa tgacgaggtc gcccggcccg
     2041 cgcgttcagg cctcacgcct cgaagtccgc ctgctgttcg ggcgcggcgg cctggaacgc
     2101 gggatgcgcc ttcgcggccg cttctgcctg aagcaggcgc ggaaaggcgg acacatccac
     2161 cttgaaccgc cgggcattgc cgagttgcgg cacgaggcag acatcggcga gactgggctg
     2221 atcgccgaaa cagaacgggc cgggctgtgc ggccaccagc ttttcgcagg cgccaagccc
     2281 ctccgcgttg acccaggcgg cccaccctgt cacctcgtcc tcggaatgcc ccatggcgcg
     2341 cagcttgttg agcaccttga ggttctgcac gggatgggtg tcgcagacga tggcgtagac
     2401 gaaggcctcg acccgcgccc gctgcaacgg ctcctgcggc agcaggctgg gctcggggta
     2461 gcgcgcttcg agccaatgga tgatggccag cgattgcgtc agcacgtcgc cgccgtcggt
     2521 caccagcgcc ggcacgagac cttgcggatt gagccggagg taggcctccc cccgctgttc
     2581 gcccctgcgc aggtgcaccg acgcgtggtc gaccgggatg cctttcaggt tcagggcgat
     2641 acggacgcgg tacgcggcgc cggaacggaa atagtcgaag agcttcatgc caggggtccg
     2701 ttgctaacgg gtgcagtctg gcacggggac gggcgcttgc aaaccgggcc ggtcggcgca
     2761 gatggcgtcc gggaccgcat gcgccgcctg ccgtgcgaat gccgcagttt ttcttggccc
     2821 tccattgagt ctaccggacg aaaaccgcca gtcgttaaaa atgcaagcgt gcgattttgt
     2881 ggattggcgc aaaccctgat ggccgatggt cgcgctcgca atgggtgcgg gcgcgacgac
     2941 agacaggatg cgctccattt cggttcacac tttcacatcc gccgcctcgg cgcgcggcat
     3001 aggctttgcg cgcgctggcg ggcgggaacg ccccggcacg caggccgtcg accgtgcact
     3061 ctggctgttg ttgcacatgg cgggggaaac cggcgccggt ctgagcctga aggatgtgtc
     3121 cgcgagcgcc ggcctcgatc cggccaccac gcatcgcctg ctcggcagcc tgaagcggtt
     3181 tgggttggtg gagcagaacc cgcagacacg gcgctatggc ctggggctgg aattcttcgc
     3241 cctggccgct gccgcctccg gacgcctaca gcttgccgaa aggatccggc cgacgctcgc
     3301 ggccttgagc agggcgtcgg gtttgcgctc ggtctgcttt gtccggaacg gtccggatct
     3361 ggtgtgtgtc gagacccatc cgggccggtc cccgtcggcc ttcggcgcgc gcgaccgcgg
     3421 tg
//
LOCUS       DAOMXN010000246         3405 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_15430_length_3405_cov_11.311940, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000246 DAOMXN010000000
VERSION     DAOMXN010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15430_length_3405_cov_11.311940"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="PK812_12880"
     CDS             <1..424
                     /locus_tag="PK812_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018064432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRJ70489.1"
                     /translation="MSPRGKPFTQAFARELAAGPGAVLVCGRFEGVDERVISARNLIE
                     ISIGDYILSGGEMAALTLLDAVVRLIPGVMGKSESGADESFENGLLEYPHYTRPRDFE
                     GQGIPEVLTSGDHAKIARWRREQAEALTRARRPDLLKK"
     gene            518..892
                     /gene="rplS"
                     /locus_tag="PK812_12885"
     CDS             518..892
                     /gene="rplS"
                     /locus_tag="PK812_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HRJ70490.1"
                     /translation="MNLIAQIEQEEAARVLQGKTIPKFQAGDTLQVNVKVVEGDRTRV
                     QAYEGVCIARSGGGFNESFTVRKISYGEGVERVFPLYSPMIESIKVVRRGTVRRAKLY
                     YLRDRRGKSARIAERVEKDKGV"
     gene            999..1880
                     /locus_tag="PK812_12890"
     CDS             999..1880
                     /locus_tag="PK812_12890"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRJ70491.1"
                     /translation="MQLHQDVINLYDRFTHSTMDRRAFFDRLTLLTGSTVAANAALAA
                     LQSNYALAQIVPENDARLAIDTLKYDGPNGPMSGYLARLKGGAKRPAVLVIHENRGLN
                     PHIKDVTRRFALEGFLAYGIDALTPEGGTPANEDEARAMFGKIDATKTAENIAKAVDV
                     LAAHAESTGKVGATGFCWGGGMTAQVAIRAEKLAAGVPYYGIQPPLDRVPAIKAPLLI
                     HYAETDERINAGIEAFRKALTDAGKSFEIHIYPGTQHAFNNDTGGARYNKAAADLAWS
                     RTVAHFKKHLGEPPKAS"
     gene            complement(1884..3089)
                     /locus_tag="PK812_12895"
     CDS             complement(1884..3089)
                     /locus_tag="PK812_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003579193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ70492.1"
                     /translation="MIVEAVKLATQAIRRNALRSFLTVLGIVIGVAAVIAMVTIGNGA
                     TAKVNADLAKLGSNLLFVGPGQFGPGRASSDARPFNARDVEAMKAQLTGVKAVAPIAQ
                     RSATVVHGGDNRIVPITGTDNDFFVTQDWALTAGRPFLESELRSGRAACIIGETVHDK
                     LFGRASPLGRSIRVGRVSCEVVGLLEAKGQSSFGIDQDDTVLMPIRTLHRRLAGNTDI
                     SRILVSADDGVETARVQADIERLLRERRNIAFGREDDFSVRDMKQIAQATAGATTILT
                     GLLGAVAAVSLLVGGIGIMNIMLVSVTERTREIGIRLAIGALERQVLSQFLIEAVVLS
                     LFGGVIGILLGLAMAYAATNGMKVPFVVDPSVVFLAFAFSALVGILFGFFPARRAARL
                     DPIEALRHE"
     gene            complement(3089..>3405)
                     /locus_tag="PK812_12900"
     CDS             complement(3089..>3405)
                     /locus_tag="PK812_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006727742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRJ70493.1"
                     /translation="PATAHPSSILACTSAELSGGQQQRVAIARAIVTDPGLLLADEPT
                     GNLDTRTSREIMELVTSLNREQGITVVMVTHETDIAAYARRTIRFVDGRIDSDTLTRE
                     AA"
BASE COUNT          584 a         1093 c         1206 g          522 t
ORIGIN      
        1 catgtcgccg cgcggcaaac cgttcacgca ggcctttgcg cgggagctcg cggccggccc
       61 cggcgcggtt ctcgtctgcg gccgcttcga gggcgtggac gaacgggtga tttccgcgcg
      121 gaacctgatc gagatttcca tcggcgacta catcctctcc ggcggcgaaa tggcggcgct
      181 gaccctgctc gacgccgtgg tgcggctcat tcccggcgtg atgggcaaga gcgaatccgg
      241 cgcggacgag agtttcgaga acgggctgct cgaatatccg cattacacgc ggccgcgcga
      301 cttcgagggg cagggcattc ccgaggttct gacttccggg gatcatgcga agattgcgcg
      361 ctggcggcgc gaacaggccg aggcgctgac gcgggcgcgg cggccggatc ttttgaaaaa
      421 atagccacgg gcgggtggac gccgcagccc tttcgctgta tggccacgcc ctcaactgaa
      481 aacagcgtgt tgaagcgctt atgacggagt aagaccgatg aacctgatcg cccagatcga
      541 acaggaagaa gccgcccgcg tcctgcaggg caagaccatc ccgaaattcc aggcgggcga
      601 cacgctccag gtcaacgtga aggtggtcga aggcgaccgc acccgcgtgc aggcctatga
      661 gggcgtctgc atcgcgcgct ccggcggtgg cttcaacgag agcttcaccg tgcgcaagat
      721 ttcctacggc gagggcgtcg agcgcgtctt cccgctgtat tcgccgatga tcgagtcgat
      781 caaggtggtg cgccgcggca ccgtgcgccg cgccaagctc tattacctgc gtgatcgccg
      841 cgggaagtcg gcccgcatcg ccgagcgcgt cgagaaggac aagggcgtgt gagggtttct
      901 cctcacgtca aagtgagcgg caaggcgcgg gtttcccgcg ccttttttgc gtgcgctcgc
      961 tagcttcccg ccagacatcc ggtgttcggg aggaccccat gcagcttcat caggacgtga
     1021 tcaatctcta cgaccgcttc acccattcca ccatggaccg gcgcgcgttc ttcgaccggc
     1081 tgacgctgct caccggctcg accgtcgccg ccaacgccgc gctcgcggcc ttgcagtcca
     1141 attacgcgct ggcgcagatc gtgcccgaga acgatgcgcg gctcgccatc gacacgctga
     1201 aatacgacgg accgaacggg ccgatgtcgg gttatctcgc ccggctgaag ggcggtgcga
     1261 agcgtccggc cgtgctggtg atccacgaga accgcggcct caacccgcat atcaaggacg
     1321 tgacgcgccg tttcgcgctg gaaggctttc tcgcctatgg catcgatgcg ctgacgccgg
     1381 agggcggcac gccggccaac gaggatgagg cgcgggcgat gttcggaaag atcgacgcca
     1441 ccaagacggc ggagaacatc gcgaaggcgg tcgacgtgct cgccgcccat gcggaatcga
     1501 ccggcaaggt cggcgccacc ggcttctgct ggggcggggg catgacggcg caggtggcga
     1561 tccgggccga gaagctggcg gcgggcgtgc cctattacgg catccagccg ccgctcgacc
     1621 gcgtgccggc gatcaaggcg ccgctgctga tccactacgc cgagaccgac gagcgcatca
     1681 acgccggcat cgaggccttc cgcaaggcgc tgaccgacgc gggcaagagc ttcgagatcc
     1741 acatctatcc cggcacgcag cacgccttca acaacgatac gggcggggcg cgctacaaca
     1801 aggcggcggc ggacctggcc tggagccgca ccgtggcaca tttcaagaag cacctcggcg
     1861 agccgccgaa ggcgagctga gccctactca tgccgcagcg cctcgatcgg atcgaggcgc
     1921 gcggcccggc gcgccgggaa gaagccgaac aggatgccga ccagcgccga gaaggcgaag
     1981 gccaggaaca ccaccgacgg gtccacgacg aagggcacct tcatgccgtt ggtggcggca
     2041 taggccatgg cgaggccgag caggatgccg atcacgccgc cgaacagcga gagcaccacg
     2101 gcctcgatca ggaactggct gagcacctga cgctccagcg cgccgatggc gaggcggatg
     2161 ccgatctcgc gcgtgcgttc cgtcaccgag accagcatga tgttcatgat gccgatgccg
     2221 ccgaccagca ggctgacggc ggccaccgcg ccgagcaggc cggtgaggat ggtggtggcg
     2281 ccggcggtgg cctgggcgat ctgcttcatg tcgcgcacgg agaaatcgtc ctcgcggccg
     2341 aaggcgatgt tgcgccgctc gcgcaggagc cgttcgatgt cggcctggac cctggcggtc
     2401 tcgacgccat cgtcggcgga aaccaggatg cggctgatgt cggtgttgcc ggccaaccgc
     2461 cggtgcagcg tgcggatcgg catgaggacg gtatcgtcct ggtcgatgcc gaagctcgac
     2521 tggcccttgg cttcaagcag gccgacgacc tcgcaggaga ccctgccgac gcggatcgag
     2581 cggccgagcg gcgacgcgcg gccaaacagc ttgtcgtgca cggtctcgcc gatgatgcag
     2641 gcggcgcggc cgctgcgcag ctcgctctcc aggaacggcc ggccggcggt cagcgcccaa
     2701 tcctgcgtga cgaagaagtc gttgtcggtg ccggtgatcg gaacgatgcg gttgtcgccg
     2761 ccgtgcacca cggtcgccga gcgttgggcg atcggcgcga ccgccttgac gccggtgagc
     2821 tgcgccttca tcgcctcgac atcgcgcgcg ttgaaaggcc tggcatcgga actggcgcgg
     2881 ccggggccga actggccggg cccgacgaac aacaggttcg agccaagctt ggcgaggtcg
     2941 gcgttgacct tggccgtggc gccgttgccg atggtgacca tggcgatgac ggcggcgacg
     3001 ccgatgacga tgccgagcac ggtgagaaac gagcgcagcg cattgcgccg gatcgcctgc
     3061 gtcgccagct tgaccgcctc gacgatcatc acgcggcctc gcgggtcagg gtgtcgctgt
     3121 cgatgcgacc atcgacgaag cggatggtgc ggcgggcata ggcggcgatg tcggtctcgt
     3181 gcgtcaccat caccacggtg atgccctgct cgcggttgag cgaggtgacg agctccatga
     3241 tctcgcggct ggtgcgcgtg tcgagattgc cggtgggttc gtcggcgagc agcaggccgg
     3301 gatcggtgac gatggcgcgg gcgatggcga cgcgctgctg ctggccgccg gagagttccg
     3361 ccgaggtgca tgcgaggatt gaggatgggt gtgctgtcgc cggct
//
LOCUS       DAOMXN010000247         3353 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_15755_length_3353_cov_13.329594, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000247 DAOMXN010000000
VERSION     DAOMXN010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15755_length_3353_cov_13.329594"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(198..1283)
                     /gene="nadA"
                     /locus_tag="PK812_12905"
     CDS             complement(198..1283)
                     /gene="nadA"
                     /locus_tag="PK812_12905"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HRJ70494.1"
                     /translation="MEGQIVEARAVSPRGDAASPATRGFVPLPRPDLTWTPEIERETR
                     HLYEKVAHVIPPVEWPFHAPYVAAINRLKKQRDAVILAHNYQTPEIYNCVADVVGDSL
                     QLARLATRTDAKVIVQGGVHFMAETSKLLNPDKTVLIPDLKAGCSLASSITGADVRQL
                     RAAYPGVPVVAYVNTSADVKAEVDICCTSSNAVQVVESLGVDRVLFVPDQYLAKYVAS
                     KTSVKIISWHGACEVHERFTGEELKRYRENDPTLKIIAHPECPPDVIEEADFTGSTAG
                     MIDYAKAQKGGRVLLVTECSMADNVAATARDVEFVRPCNLCPHMKRITLPKILDSLLY
                     MQHEVTIDPLLAERARRSVERMVNLKN"
     gene            1626..2855
                     /locus_tag="PK812_12910"
     CDS             1626..2855
                     /locus_tag="PK812_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRJ70495.1"
                     /translation="MSEAATIRRISPVTIIVAGCLISMIGFAVRSVMGFFQLPVITEY
                     GWGRDVFGLALAVQNLFWGIGQPFAGAVADRFGTVRVLIVGMLAYAAGLVLMTVSSTP
                     SAFVLTAGVLFGLALAGCSFNLVLAAFGKLLPAEWRGTAFGVGTAAGSFGQFLFSPLA
                     VGLIDSVGWRGALLVFAGLMLVSIPLALALRTDGLATGAQAGGEPHPGLASTLAHAFG
                     HPSYILLVLGFFTCGFHLAFITVHLPAHLKDGGLNPAIGGWVVACIGLFNVVGSLMSG
                     WLCSRVPRRYLLAVIYAARSVAIAFFFFLPLTPVTAILFGASMGLLWLSTVPPTSSLV
                     ATMFGTRDMAMLYGFVFFSHQVGGFLGVWLAGLLYESTGTYDLIWYASMALGIASAAV
                     NLPIVEAAAPSRRPAAA"
     gene            2932..>3353
                     /locus_tag="PK812_12915"
     CDS             2932..>3353
                     /locus_tag="PK812_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020096232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HRJ70496.1"
                     /translation="MTQDFRALVVLKDEAGYRCEERRLTNTALMDGDVTVAVSHSTVN
                     YKDGLAITGKSPVVRKFPLVPGIDFTGTVESSTDARYKPGDAVVLTGWGVGETHSGGY
                     AEKARVKGDWLVPLAAGLTPARAMAIGTAGFTAMQSIVA"
BASE COUNT          519 a         1080 c         1095 g          659 t
ORIGIN      
        1 gccctcgcac agcggtttcg gcgcgacgat tgtgcaggcc tgcggactga gcttcagcgc
       61 cgcgaacaat ccggcaaggc cgccgccgag gatgaggatc gaggacatag gcggctttcg
      121 ctctgcatca cgaccagaaa tcccgccccg cgtacggcgg gagcgcgcac gacatcgatc
      181 cgcgccgccc gcccgcgtca attcttcagg ttgaccatgc gctcgaccga acggcgcgcg
      241 cgctcggcga gcaggggatc gatggtcacc tcgtgctgca tgtaaagcaa gctgtcgaga
      301 atcttcggca gcgtgatgcg cttcatgtgc gggcacaggt tgcaggggcg cacaaattcc
      361 acgtccctgg cggtcgcagc gacattgtcg gccatcgagc attcggtgac gagcagcacg
      421 cggccgccct tctgcgcctt ggcatagtcg atcataccgg cggtcgagcc ggtgaaatcg
      481 gcctcctcga tcacatcagg cgggcattcc ggatgggcaa tgatcttcag cgtcggatcg
      541 ttctcgcggt agcgcttgag ctcctcgccg gtgaagcgct cgtgcacctc gcaggcgccg
      601 tgccaggaga tgatcttcac cgacgtcttc gaggcgacgt acttcgccag atactgatcc
      661 ggtacaaaca gcacgcggtc gacaccgagg ctctccacca cctgcacggc attggaggag
      721 gtgcagcaga tatcgacttc cgccttcacg tcggcagagg tgttgacgta ggcaaccacc
      781 ggcacgccgg gataggcggc gcgcaactgg cgcacgtccg ccccggtgat cgaggacgcg
      841 agcgagcagc cggccttgag gtcggggatc agcaccgtct tgtccggatt gagcagcttc
      901 gacgtctcgg ccatgaaatg cacgccgccc tgcacgatca ccttcgcatc ggtcctggtc
      961 gcgaggcggg cgagttgcag cgaatcgccg acgacatcgg cgacgcaatt gtagatttcc
     1021 ggcgtctggt aattatgcgc gaggatgacc gcatcgcgct gcttcttcag acggttgatc
     1081 gcggcgacgt acggcgcatg gaacggccat tccaccggcg gaatgacatg cgcgaccttc
     1141 tcgtagagat gacgcgtctc gcgctcgatc tcaggcgtcc aggtcaggtc gggacgcggc
     1201 agcggcacga agccgcgcgt ggcgggcgat gcggcatctc cacgcgggga gacggcgcga
     1261 gcttcgacga tctgaccttc cataacacac ctcaaatgct cattatgagc ataactaggt
     1321 ttggttccgc cgccgcgatt tgacgccgtg aaggttattc gcctgttcac ttcgagggaa
     1381 ttcccgcgac atcagaaccg gagaaccgcc acgccgatca tgctcgggcc gacagttata
     1441 ctcaacttga gaattattat aggcgggttt gccggctttg gaaagaggga aatttcaatg
     1501 cgttcgaggg ccatggtccg gagcattcag acgcaggcat tcgttttctt gatcggcctc
     1561 atcaaagaat gcgggttttc gccggtcctg cttgccgcta ccactgtgac caagagggat
     1621 ccgcaatgtc cgaagctgcc accatccgcc gcatctcgcc cgtgaccatc atcgtcgccg
     1681 gctgcctgat ctcgatgatc ggctttgccg tgcgctcggt catgggtttc tttcagctac
     1741 cggtgatcac ggaatacggc tggggacgcg atgtcttcgg cctggcgttg gcggtgcaga
     1801 acctgttctg gggcattggc cagcctttcg ccggcgcggt ggcggaccgt ttcggcacgg
     1861 tgcgcgtgct gatcgtcggc atgctggcct atgccgccgg cctggtgctg atgacggttt
     1921 cgtcgacgcc ttccgccttt gtgctgacag cgggcgtgct gttcggcctg gcgctcgccg
     1981 gctgctcgtt caacctggtg ctggccgcct tcggcaagct cctgccggcg gaatggcgcg
     2041 gcacggcatt cggggtgggc actgcggccg gctctttcgg acagttcctg ttttcgccgc
     2101 tggcggtcgg tctgatcgat tcggtcggct ggcggggcgc tctgctggtg ttcgccgggc
     2161 tgatgctggt ctcgatcccg ctcgccctgg cgctgcgtac ggacggcttg gcgaccggcg
     2221 cgcaagccgg cggcgaaccg catccgggcc tcgcctcgac gctcgcccat gccttcgggc
     2281 atcccagcta catcctgctt gtcctggggt tcttcacctg cggattccac ctggccttca
     2341 tcaccgtgca cctgccggcg cacctgaagg acggtgggct caacccagcg atcggcggct
     2401 gggtggtggc gtgcatcggc ctgttcaacg tcgtcgggtc gctgatgtcg ggctggctgt
     2461 gcagccgggt cccgcgccgc tacctgctgg cggtgatcta cgcggcgcgc tccgtcgcca
     2521 tcgccttctt cttcttcctg ccgctgacgc cggtcacggc catcctgttc ggcgcatcga
     2581 tgggcctgtt atggctgtcg accgtgccgc caacctcctc gctggtcgcc accatgttcg
     2641 gcacgcggga tatggcgatg ctctatggct tcgtgttctt ctcccaccag gtcggcggct
     2701 tcctcggcgt ctggctggcc ggcctgctct acgaaagcac cggaacctac gacctgatct
     2761 ggtatgcctc gatggcgctg ggcatcgcct cggcggccgt caacctgccg atcgtcgagg
     2821 cggctgcgcc gtcgcgcagg ccggccgcag cctgagcttc gaccattggt ggatgacggg
     2881 cgcgcggcgt ccggatagtc tggcggttcc attcgaaccg ggaccttgga catgacacag
     2941 gatttccgcg cgctggtcgt cctcaaggac gaggcgggct atcgctgcga ggagcgccgt
     3001 ctgaccaaca ctgccctcat ggacggcgac gtcaccgttg cggtgagcca ttccaccgtg
     3061 aactacaagg atggccttgc catcaccggc aagtcgcccg tcgtacgcaa gttcccgctg
     3121 gtgccgggca tcgacttcac cggaacggtc gaatcgtcga ccgacgcccg ttacaagccg
     3181 ggcgatgccg tcgtgctcac cggctggggg gtgggcgaga cccattccgg cggctacgcc
     3241 gaaaaggcgc gagtgaaggg cgactggctg gtgccgctgg cggccggcct gacgccggcg
     3301 cgcgccatgg cgatcggcac ggcggggttc accgccatgc agagcattgt cgc
//
LOCUS       DAOMXN010000248         3353 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_15757_length_3353_cov_9.905397, whole genome shotgun sequence.
ACCESSION   DAOMXN010000248 DAOMXN010000000
VERSION     DAOMXN010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15757_length_3353_cov_9.905397"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..751)
                     /locus_tag="PK812_12920"
     CDS             complement(131..751)
                     /locus_tag="PK812_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRJ70497.1"
                     /translation="MLRLWGRLNSINVQKAAFALEEAGAPYERIDAGMAFGIVGTPEY
                     RAKNPNGLIPLLEDGDFTVWESNVIVRYVAAQYASGTLWPLDARARASADKWMDWQQT
                     TLVVALGPAFLGLIRTPPEQRDAKAIEASVAKTDELMGRLNAALSATSHVAGERFTMG
                     DIPIAATVHRWLNLPIARTDYPAIKAYYARVMARPAAQKTLILPVT"
     gene            905..1621
                     /locus_tag="PK812_12925"
     CDS             905..1621
                     /locus_tag="PK812_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HRJ70498.1"
                     /translation="MTADVRTLHPDPNGAGLSRSARLRGYFLTGLVVAGPLAITVWLS
                     WWFINMIDGWVKPFVPRTFWPETYLPFPIPGFGVIILLFALTMLGFLTANLVGRTMLD
                     LGENLLNRMPVVRGIYKSAKQIFETIFSQSGTSFRRVGLIEYPGPGLWSIVFISSPPT
                     GDIAAKLPGGPDDQIGVFLPCAPNPTTGFFFYVARSQLREIQTSVDDAFKIVLSLGLL
                     KDESQQEKLRDLAAQSKMVT"
     gene            complement(1638..2009)
                     /locus_tag="PK812_12930"
     CDS             complement(1638..2009)
                     /locus_tag="PK812_12930"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006466517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HRJ70499.1"
                     /translation="MPAPDEDNEDQAVTIADNFAATPAPPYYVVTFTSQHSDDDDGYA
                     AMADRMVELAARQPGYLGIESVRDASGFGITLSYWTSLDAITAWKRNAEHLEAQRRGR
                     AGWYKRFELRVARVEKAYGKR"
     gene            complement(2042..>3353)
                     /locus_tag="PK812_12935"
     CDS             complement(2042..>3353)
                     /locus_tag="PK812_12935"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HRJ70500.1"
                     /translation="VRAALPYSLTGAQERATAAIAGDMASGERMLRLLQGDVGSGKTV
                     VGLLAMTAAVESGRQAAMMAPTEILARQHFDRLKPLCDAAGMRIALLTGRSKGGERAA
                     VLAGLADGGIDIAVGTHALFQDDVAFRDLALAVVDEQHRFGVHQRLALSRKGEATDLL
                     VMTATPIPRSLVLTYFGDMDVSILDEKPAGRQPIDTRLIDLDRLGDVANSLGRAIAGG
                     ARIYWVCPLVAESEKSDLAAATDRFEDLDRLFPGRVGLVHGQMKGADKDEAVRRFQAG
                     ESDILVATTVIEVGVDVPEASIMVVEHAERFGLAQLHQLRGRIGRGAAKSTCLLLYKA
                     PLGEVAQARLKILRETDDGFRIAEEDLKLRGEGDVLGTRQSGSPGFRIARLERHGPLL
                     RMARDDAELVLARDPELASPRGEALRLLLHLFERDEAVRLFRAG"
BASE COUNT          522 a         1093 c         1137 g          601 t
ORIGIN      
        1 gtcgcccttc gccgtcgatt ccgcgcgaat gacatagggc aggtcgttgt gcgcatcgac
       61 gagggcgatg cgatcgagaa aatcgcgggc gcgggagagg ctggatctgc gggcgggcat
      121 ggcgggtgac tcaggtgacc ggcaggatga gcgttttctg tgccgccggg cgcgccatga
      181 cgcgcgcata gtaggccttg atggctgggt agtccgtgcg ggcgatggga aggttcagcc
      241 aacggtgcac cgtcgcggcg atggggatgt cacccatggt gaagcgctcg ccggcgacgt
      301 gagatgtcgc cgagagcgcg gcgttcagcc ggcccatcag ctcgtcggtc ttcgccactg
      361 acgcctctat ggcctttgcg tcgcgctgtt cgggtggggt gcggatcagg cccaggaatg
      421 ccgggccgag cgccacaacc aacgtcgtct gctgccaatc catccacttg tcggcactgg
      481 cgcgagcccg ggcgtcgagc ggccagagcg tgccagaggc gtattgcgcg gcgacatacc
      541 ggacgatgac attggattcc cacaccgtga aatcgccgtc ttcgagcagt ggaatgagcc
      601 cgttcgggtt cttcgcccgg tactctggcg taccgacgat gccgaaggcc atgccggcgt
      661 cgatgcgctc gtagggcgcg ccggcctcct caagggcgaa ggcggctttc tgcacgttga
      721 tcgaattgag ccgtccccaa agcctgagca tgcgattcct ccgcgttggc cgcattgcag
      781 catccttccg tccggcacgg aaggggccgg atggcgaaag cgatgccgcg cggtggccga
      841 attgacgaat tttctttcgc cgcattccct gatatgcgcg accggaaggc cccgacgagg
      901 cgacatgacc gctgacgtgc gcaccctgca tcccgatccc aacggcgccg gattgtcccg
      961 ctcggcccgc ctgcgcgggt atttcctcac cgggctggtg gtggccgggc cgctggcgat
     1021 cacggtctgg ctgagctggt ggttcatcaa catgatcgac ggctgggtga agccgttcgt
     1081 tccgcgcacg ttctggccgg agacctatct gccgttcccg attccgggct ttggcgtcat
     1141 catcctgctg tttgccctga ccatgctggg gttcttgacc gccaacctgg tcggtcggac
     1201 catgctcgac cttggcgaga accttctcaa ccgcatgccg gtggtgcgcg gcatctacaa
     1261 gagcgccaag cagattttcg agacgatctt ctcgcagtcc ggcacctcgt tccgtcgtgt
     1321 cgggctgatc gagtatcccg ggcctgggct gtggtcgatc gtgttcatat cctcgccgcc
     1381 gacgggcgac atcgccgcca agcttccggg cgggccggac gaccagatcg gcgttttcct
     1441 gccctgcgcg cccaatccga cgacgggctt tttcttctac gtcgcgcgct cgcaactgcg
     1501 cgagatccag acgtcggttg atgacgcctt caagatcgtg ctgtcgctcg gcttgctgaa
     1561 ggacgagagc cagcaggaaa agctgcgcga cctggcagcg caatcgaaga tggtgacgtg
     1621 atcgcgcacg ggcgccctca ccgtttgccg taggcttttt ccacgcgcgc tacacgcaat
     1681 tcgaatcgct tgtaccagcc ggcccggccg cgccgttgcg cttccagatg ctcggcgttg
     1741 cgtttccagg cggtgatcgc gtccagtgag gtccagtagc tcagggtgat gccgaatccg
     1801 gacgcatcgc gcacgctttc aatgccgagg tagcccggtt ggcgcgcggc aagctccacc
     1861 atgcgatcgg ccatggcggc atagccgtcg tcgtcgtcgc tgtgttggct cgtgaaggtg
     1921 acgacgtagt agggcggggc gggtgtcgcg gcgaagttgt cggcaatggt cacggcctga
     1981 tcctcgttgt cctcatcggg cgcgggcatt ggagcaatat ccggtctggt cggatactgc
     2041 tctagcccgc tcgaaacaag cgcacggctt cgtcccgctc gaaaaggtgc agcagcagcc
     2101 ggagcgcctc gccacgcggc gaggccagtt ccggatcgcg cgccagcacc agttcggcat
     2161 catcgcgtgc catgcgcagc agcgggccgt ggcgctccag ccgggcgatg cgaaagccgg
     2221 gactgccgga ttgcctggtg cccagaacgt cgccttcgcc gcgcagtttc aggtcctcct
     2281 cggcgatgcg gaagccgtcg tcggtctcgc gcaggatctt cagccgcgcc tgagccacct
     2341 cgccgagcgg cgccttgtag agcagcaggc aggtggattt ggccgcaccg cgcccgatgc
     2401 ggccgcgcag ttggtggagc tgcgccagac cgaagcgttc cgcatgctcc accaccatga
     2461 tcgaggcctc gggcacgtcg acgccgactt cgatgaccgt ggtggcgacg agaatgtcgc
     2521 tttcgccggc ctggaagcgg cgcacggcct catccttgtc cgcacccttc atctggccgt
     2581 gcacaaggcc gacgcggccg ggaaacagcc gatcgaggtc ctcgaaacgg tccgtcgcgg
     2641 cggcgaggtc gctcttctcg ctttccgcca ccagcggaca gacccagtag atgcgcgcgc
     2701 cgccggcgat ggcgcggcca aggctgttcg ccacatcgcc cagccggtcg agatcgatca
     2761 ggcgggtgtc gatgggctgg cggccagccg gcttctcatc gaggatcgag acgtccatgt
     2821 cgccgaagta ggtgagcacc agagagcggg ggatcggcgt cgcggtcatc accagcaggt
     2881 ccgtggcctc gcccttgcgc gacagggcaa ggcgctgatg cacgccgaag cggtgctgtt
     2941 cgtccaccac tgccagcgcg agatcgcgga aggcgacatc gtcctggaac agcgcatggg
     3001 ttcccaccgc gatgtcgatg ccgccatccg ccagtccggc cagcacggcg gcacgctccc
     3061 cgcctttcga gcggccggta agcagggcga tgcgcatgcc tgcggcgtcg cagaggggct
     3121 tcagacggtc gaaatgctgg cgcgcgagga tttccgtcgg cgccatcatc gccgcctgcc
     3181 ggccggactc gaccgccgcc gtcatggcca gcaggccgac cacggtcttg ccggagccga
     3241 catcgccctg gagcaggcgc agcatgcgtt cgccgctggc catgtcgcct gcgatggcgg
     3301 ctgtcgcccg ttcctgcgca ccggtcaggg aataaggcag agcggcgcgg acg
//
LOCUS       DAOMXN010000249         3350 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_15783_length_3350_cov_10.321700, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000249 DAOMXN010000000
VERSION     DAOMXN010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15783_length_3350_cov_10.321700"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="PK812_12940"
     CDS             <1..626
                     /locus_tag="PK812_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70501.1"
                     /translation="AGTRGGSRAPNIAAALLAVAALAGGCVPLSGDLGRPRPLLHASR
                     LEGFAAPVTRVEQEREDRLVHFRRAAPPLPGRSASAAFNALRDHIETDRALLPPLRAA
                     LAGSADKQRIRAVALGVFPSVDTHARAESQARIVEDKVAAGEFCPLLRSRAQGHRAQL
                     EALVVHAPEVEAIAAERSLVALESEIAQACVSAVEPGAVSPPMRIRK"
     gene            complement(636..1190)
                     /locus_tag="PK812_12945"
     CDS             complement(636..1190)
                     /locus_tag="PK812_12945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70502.1"
                     /translation="MIVAFARILLVLVVAGLTHLTSVLAMPWLAPKAGYMRLVPLAKG
                     NEMTLLPARDLQQAIPFWDPAVAAAACRYVLDTGPVRIRAAVGGSSMAVLFLRKGVGV
                     FQSYSDRAATQGILEMVIATPEQMRQITALDADDEPVQEIRVLSEDDTGLVLVKAVVP
                     TASQRADVEALVGAATCEAESLER"
     gene            complement(1187..1765)
                     /locus_tag="PK812_12950"
     CDS             complement(1187..1765)
                     /locus_tag="PK812_12950"
                     /inference="COORDINATES: protein motif:HMM:NF018452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HRJ70503.1"
                     /translation="MTNLVWTFLALLAGIAGGLGVTAWSVDSPPNFGVVEAGAWITRP
                     KVGSTEADPYSRALHSAQGTIPLGASEGVELTAAVDSAGRRLTAECAYRFHGPVPASR
                     YWTLTAYRHDGRLVGDGERRSAFTSAEVVRNQSGNAEIWLAATPRPGNWIPLPPDGAI
                     DLSLRLYDTQFSSNARAIDAASVPRLDRESCQ"
     gene            complement(1895..>3350)
                     /locus_tag="PK812_12955"
     CDS             complement(1895..>3350)
                     /locus_tag="PK812_12955"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRJ70504.1"
                     /translation="RARAGDVLSAMVDAGFMTEGQVAAARRNPASPVPQTRESSPDFF
                     LDFAFDEVRRLAEDGKLGNDRILRVRTTIDLGLQKHAEQAHETILRRDGPNYDVKQSA
                     SVTVEHDGAVRALVGGRDYGASQFNRATHALRPPGSSFKPVVYAAAMVHTGIKPAVKV
                     TDRPTCVGNWCVNNYSRSYAGTVDLATALARSYNTIPVQLTTAIGKGNNKAGRVAVIE
                     MARRLGLGDLRDMTSLPIGSTEVTVVDMASAFAVFANGGKRARAYAVIEVRNSQNALI
                     YELPRPEVRNPQIIPASVAADMNYMLNKVTEEGTGRRALLPGIKTAGKTGTSDEYKNA
                     WFVGYTGNYTSAVWFGNDDATPTSNMTGGSLPAQTWHDIMEYAHTDIDLKPMYGIDVE
                     PAKPAGMAANVPVPKAKPGSADLQASVRNTRLSRKGAQAVQELETLFRNEVERQRSAE
                     APGPQGQPGTPQTPARRASFQPLTGAGTAVTRLN"
BASE COUNT          525 a         1102 c         1147 g          576 t
ORIGIN      
        1 aggccggcac cagagggggt tcgagagccc cgaacatcgc ggcggcgctg cttgccgttg
       61 cggcactggc cggcggctgc gtgcccttgt cgggtgacct tggacggccg cgtccgctac
      121 tccacgcatc gagactggaa ggcttcgccg cgcccgtgac ccgcgtcgag caggagcgcg
      181 aggaccgcct cgtacatttc cggcgtgccg ctccgccact tcccggacgc tccgcgtctg
      241 ccgccttcaa cgcgttgcgc gaccatatcg agacagaccg cgccctgctg ccgccgttgc
      301 gcgcggcgct ggcggggagc gccgacaagc agcgcatccg cgccgtggcg ctgggtgtct
      361 ttccttcggt tgacacgcac gcgcgcgccg aaagccaggc ccgcatcgtg gaagacaagg
      421 tcgcggccgg cgagttctgc ccgcttctgc gctctcgtgc gcaaggccac agggcgcagc
      481 ttgaagccct tgttgttcat gcccccgaag tcgaggcgat cgcagcagaa cgcagcctcg
      541 tggcgttgga aagcgagata gcgcaggctt gcgtcagcgc ggtcgagccg ggcgccgtca
      601 gcccgccgat gcgaattcgc aagtagtcat gcccgttacc gctcgaggct ttccgcctcg
      661 caggtcgcgg caccgaccag cgcctcgaca tcggcacgct gggaggccgt gggaaccacc
      721 gccttgacca gcaccagccc ggtatcgtct tcggacagga cacggatttc ctgcaccggt
      781 tcgtcatccg cgtcgagcgc ggtgatctgg cgcatctgct ccggcgtggc gatgaccatc
      841 tcaagaatcc cctgtgtcgc ggcccggtcc gaatagctct ggaacacgcc gacgcccttg
      901 cgcaggaaca gcaccgccat ggaggaaccg ccgaccgcgg cgcggatgcg caccgggcct
      961 gtgtcgagca cgtagcgaca ggccgcagcc gcaacagcgg ggtcccagaa cggtatcgcc
     1021 tgctgcaggt cgcgcgctgg cagaagcgtc atctcgttac ccttggccag gggaacgagc
     1081 cgcatgtagc cggccttcgg cgcgagccag ggcatggcaa ggacgctggt cagatgcgtc
     1141 aatccagcca cgaccagaac gagcaggatg cgggcgaagg caacaatcat tgacaggact
     1201 cgcggtcaag gcgcggtacg ctggctgcgt cgatggcgcg ggcgttgctg ctgaactgcg
     1261 tgtcgtagag acgtagcgaa agatcgatgg ctccgtctgg cggcaacgga atccagtttc
     1321 ccggccgggg tgtcgccgcc agccagatct ccgcattgcc gctctggttg cggaccactt
     1381 ccgccgaggt gaacgcggag cgtcgctcgc cgtcgccgac cagtcggcca tcgtggcggt
     1441 aagccgtcag cgtccagtag cgcgaggccg gaaccggtcc gtggaagcgg taggcgcact
     1501 ctgctgtcag ccggcgtccg gcggaatcga cagcagcggt caattccacg ccttccgacg
     1561 cgccaagcgg aatcgtgccc tgtgcggaat ggagggcccg ggagtagggg tcggcctcgg
     1621 tcgatccgac cttgggccgg gtgatccagg caccggcctc gaccacgccg aaattgggcg
     1681 gcgagtccac cgaccaggcc gtcaccccaa gtccgccggc gatgccggcg agcagggcga
     1741 ggaatgtcca gacgagattt gtcaaagtcg gaatcgctgt tcagccagtg cgcgccacca
     1801 tcttagcaaa agcgcgcgcc cggcctaccg ggaataacgc tggccgcgtc cggaccgcag
     1861 tcgcggggac gccatggatt tcggacggcg tggtctagtt gaggcgtgtg acggcggtgc
     1921 cggcgccggt caacggctgg aaggaggcgc gtcgagctgg cgtctgcggt gtgcccggct
     1981 gcccctgcgg tcccggtgct tccgcgctgc gctggcgttc gacctcgttg cggaacagcg
     2041 tttcaagttc ctgcaccgcc tgcgctccct tgcggctgag gcgggtgttg cgcaccgaag
     2101 cctgcaaatc cgccgagccg ggcttggcct tggggactgg aacattggcc gccatcccgg
     2161 ccggtttggc gggctcgaca tcgatgccgt acatcggctt caggtcgatg tcggtatggg
     2221 catattccat gatgtcgtgc caggtctgcg ccgggaggga cccgcccgtc atgttggagg
     2281 tcggggtcgc gtcatcgttg ccgaaccaga cggcggacgt gtagttcccg gtgtatccga
     2341 cgaaccaggc gttcttgtat tcgtccgacg tgccggtctt gccggcggtc tttatccccg
     2401 gcagcagcgc ccgccgcccc gtgccttctt ccgtcacctt gttcagcata tagttcatgt
     2461 cggctgcaac ggatgccgga atgatttgcg gattgcggac ctcggggcgc ggcagctcat
     2521 aaatgagcgc attctggcta ttgcggactt cgatcacggc ataggccctc gcccgtttgc
     2581 cgccgttggc gaagacggca aatgccgagg ccatgtcgac caccgtcacc tcggtcgacc
     2641 cgatcggcag cgaagtcatg tcgcgaaggt cgccaagccc cagccggcgc gccatctcga
     2701 tcaccgcgac tcgccccgcc ttgttgttgc ccttgccgat ggcggtggtg agctgcacgg
     2761 gaatggtgtt gtaggagcgt gccagagcgg tcgccagatc caccgtgccg gcataggagc
     2821 gcgaatagtt gttcacgcac cagttcccga cgcaggttgg ccgatccgtg acctttaccg
     2881 ccggcttgat gccggtgtga accatggccg cggcatagac cacgggcttg aacgacgatc
     2941 cgggcgggcg gagggcatgc gtggcccggt tgaactggct ggcgccgtag tcgcgtccgc
     3001 cgacaagggc gcgtacggcg ccgtcgtgct ccacggtcac ggaggcggat tgcttgacgt
     3061 cgtagttggg tccatcccgc cgcaaaatgg tctcgtgcgc ctgttcggca tgtttttgca
     3121 ggccaaggtc gattgtcgtc ctgaccctga ggatgcggtc gttgccgagc ttgccgtcct
     3181 ctgccagccg ccgcacctcg tcgaaggcga agtcgaggaa gaagtcggga gaggactcgc
     3241 gcgtttgcgg caccggcgag gccggattgc gccgcgccgc cgcgacctgc ccctcggtca
     3301 tgaagccggc atcgaccatg gcgctcagca cgtcgccggc ccgggcgcgc
//
LOCUS       DAOMXN010000250         3298 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_16124_length_3298_cov_8.699044, whole genome shotgun sequence.
ACCESSION   DAOMXN010000250 DAOMXN010000000
VERSION     DAOMXN010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16124_length_3298_cov_8.699044"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..437
                     /locus_tag="PK812_12960"
     CDS             141..437
                     /locus_tag="PK812_12960"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRJ70505.1"
                     /translation="MPVAGRILLPGGREVAVSGRIDRLLITPERIVIADFKTGRADAD
                     GTAHATQLALYRDLLRRMFPGRPVECLLVWTRSQRIERIDDDALDAALAAFTPP"
     gene            511..834
                     /gene="trxA"
                     /locus_tag="PK812_12965"
     CDS             511..834
                     /gene="trxA"
                     /locus_tag="PK812_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018264919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HRJ70506.1"
                     /translation="MSAAAKVTDQSFDADVLKSGQPVVVDFWAEWCGPCRMIAPSLDQ
                     IAAEMAGKVKVVKLNVDENPNMAAKLGIRSIPTLMLFKDGKLAATKVGAASKGDLQKW
                     ITASI"
     gene            complement(838..2157)
                     /locus_tag="PK812_12970"
     CDS             complement(838..2157)
                     /locus_tag="PK812_12970"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HRJ70507.1"
                     /translation="MRLSEQYLQRFAALHPILIDLSLGRIQRLLEKLGHPERRMRHVI
                     HVAGTNGKGSTIAFMRAMLEADGRRVHVFTSPHLVRFHERIRIGRVGGGRLVEEELLV
                     AAFEHVERINAGDPITFFEITTAAAFHLFAEHPHDACLIEVGLGGRFDATNVFAKPAA
                     TVIAPVSMDHREHLGDTIGQIAGEKAGILRRGAPAVMAPQSEAAGRTLQQAAERVGAS
                     PIVIGGQDFSVHEEHGRLVYQDEQGLLDLPLPRLRGRHQHVNAATAIATLRTVYGAGF
                     PAASLEKGLSSADWPARLQRLSGALAEMAPAGAELWLDGGHNEDGARALAESLAEMEE
                     RHPRPLILITGLMARKDANAILEPFRGLAQELYCVDIPGEKLARPGSDLAAIGRSLGM
                     ACAVAGTVPETLRFLAVRAWQTSPRILIAGSLYLAGEVLAADGSEPK"
     gene            complement(2208..3074)
                     /gene="accD"
                     /locus_tag="PK812_12975"
     CDS             complement(2208..3074)
                     /gene="accD"
                     /locus_tag="PK812_12975"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020093040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HRJ70508.1"
                     /translation="MNWITNLVRPKIRNLLGKRETPENLWIKCPDSGQMVFHKDVEAN
                     LWVIPGSEHHLKMSALQRLTWMFDGGAFEDIAVRETTVDPLKFRGKDRYVDKLKEARA
                     KSGLNDAVKLGFGKLDGLPVTIGAHDMDFIAGSLGMAAGEAVISGLETAAARGTPFVL
                     FTASGGARMQEGILSLMQLPRTTVAVRRLRAARKPYIVVLTNPTTGGVTASYAMLGDV
                     HLAEPGALIGFAGPRVIEQTVREKLPEGFQRAEYLRDHGMVDMVVHRKDMRATLSNLC
                     RLLTKAPPAAAA"
     gene            complement(3102..>3298)
                     /locus_tag="PK812_12980"
     CDS             complement(3102..>3298)
                     /locus_tag="PK812_12980"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HRJ70509.1"
                     /translation="VKAPEHAAAIARGADGVVVGSALVDAVRASLDADGKATGATVPA
                     VSALVQGLAGAVRATKRTLA"
BASE COUNT          556 a         1128 c         1085 g          529 t
ORIGIN      
        1 tggcaagacc atgctcgcct tcgccccggc cgagaacctc gtcgccatcg ccgacgcggc
       61 acaggctggt gggcgaagcg ctcgccttga tgcgcacgcc cggggcgacc gccatcttcg
      121 gcctgggatc ccgcgcggag gtgccggtcg ccgggcgaat cctcctgccg ggcgggcggg
      181 aagtcgcagt ctcggggcgc atcgaccgac tgctgatcac gcccgagcgc atcgtcatcg
      241 cggacttcaa gaccggccgc gccgatgcgg acggcacggc ccatgccacg cagctcgccc
      301 tctacaggga tctgctacgc cggatgtttc ccggccggcc cgtggagtgc cttcttgtct
      361 ggacgcgcag ccagcgcatc gaacggattg acgacgatgc gctcgatgcg gcgcttgcgg
      421 ccttcacgcc gccgtgaccc gccttgattc gtcgaggcct catgcctagc ttctatcttg
      481 gattctcccc tcgcagatga aggatacgcc atgagcgccg ccgccaaagt caccgaccag
      541 agcttcgatg ccgatgtgct gaagtcgggc caacccgtcg tggtcgattt ctgggctgag
      601 tggtgcggac cctgccggat gatcgcgccc tcgctggatc agatcgcggc ggaaatggca
      661 ggcaaggtga aggtggtgaa gctcaacgtc gatgaaaacc ccaacatggc ggcgaaactg
      721 ggcatccgct cgatcccgac gctgatgctg ttcaaggacg gcaagctggc ggccaccaag
      781 gtgggcgcag cctccaaggg tgacctgcag aagtggatca cggcctcgat ctgaggacta
      841 tttcggttcc gacccgtcgg cggcaagcac ctcgcccgcc aggtagagcg accccgcgat
      901 aagaatgcgc gggctcgtct gccaggcacg gaccgcgaga aagcgcaggg tctccggcac
      961 ggtgccggca acggcacagg ccatgccgag cgaacgcccg atggccgcga gatccgatcc
     1021 gggccgcgcc agtttctcgc ccggaatgtc gacgcaatag agttcctgcg ccaggccgcg
     1081 gaacggctcc aggattgcgt tggcatcctt gcgcgccatc aggccagtga tcaggatcag
     1141 cggtcgcgga tgacgctcct ccatctcggc gagcgactcg gcaagggcac gtgccccgtc
     1201 ttcattgtgg ccgccgtcca gccagagttc ggcgccagcc ggcgccatct ccgccagagc
     1261 gccggacaac cgctgcaacc gcgccggcca atccgctgat gaaaggccct tttccagcga
     1321 cgcggccggg aaccctgctc cgtaaacggt ccgcagcgtg gcgatcgccg tggcggcgtt
     1381 gacatgctgg tgccgtccgc gcaggcgcgg caacggcaga tccagcagcc cctgctcatc
     1441 ctgatagacc agacggccgt gctcctcgtg gaccgagaag tcctggccgc cgatgacgat
     1501 gggcgacgcg ccgacccgtt cggccgcctg ttgcagtgtg cggccggccg cctcggactg
     1561 cggggccatc acggcgggtg cgccgcgccg cagaattccg gccttttctc cggcaatctg
     1621 cccgatggtg tcgccgaggt gctcgcgatg atccatcgat accggtgcaa tgacagtcgc
     1681 cgccggcttc gcgaagacat tggtcgcatc gaagcggccg ccgaggccaa cctcgatcag
     1741 gcaggcatcg tgcgggtgct ccgcgaacag gtggaaggcc gccgcggtgg tgatctcgaa
     1801 gaaggtgatg ggatcgccgg cgttgatgcg ttcgacatgt tcgaaagcgg caaccagaag
     1861 ctcctcctcc accagccggc cgccgccaac ccgcccgatg cgaatacgct cgtggaagcg
     1921 caccagatgc ggcgaggtga acacatgcac ccgccggcca tccgcttcca gcatggcgcg
     1981 catgaacgca atggtcgagc ccttgccgtt ggtcccggcg acgtggatga catggcgcat
     2041 gcgccgctcg ggatggccga gcttttccag aaggcgctgg atgcgcccca gcgacaggtc
     2101 gatcaggatc ggatggagcg cggcgaaccg ctgcaggtac tgttccgaaa ggcgcatgtc
     2161 ggcttacgcc ctgtgcatgg gatcgcgcat cgccggcacc ggcagtccta ggctgcggca
     2221 gccggcggtg ccttggtcaa caggcggcag aggttcgaga gggtggcccg catgtccttg
     2281 cgatgcacca ccatgtcgac catgccgtga tcgcgcagat attccgcgcg ctggaagcct
     2341 tccgggagct tttcgcgcac ggtctgctcg atcacgcgcg ggccggcaaa gccgatcagc
     2401 gcgccgggct cggccaggtg cacatcaccc agcatggcgt aggaggccgt aacgccgccg
     2461 gtggtcgggt tcgtcagcac gacaatgtag ggcttgcgcg ccgcgcgcag ccgccgcacc
     2521 gccaccgtgg ttcgcggcag ttgcatcagc gacaggatgc cctcctgcat gcgtgcgccg
     2581 cccgaagcgg taaacagcac gaagggcgtg ccgcgcgccg ccgccgtttc cagcccgctg
     2641 atcacggcct cgcccgccgc catgccgagc gaaccggcaa tgaagtccat gtcatgtgcg
     2701 ccgatcgtca caggcagccc gtccaacttg ccaaagccga gcttcaccgc atcgttgagg
     2761 ccggacttcg cgcgcgcctc cttcagcttg tcgacatagc ggtccttgcc gcggaacttg
     2821 aggggatcga ccgtcgtctc gcgcacggcg atgtcctcga aggcgcctcc gtcgaacatc
     2881 caggtcaggc gctggagggc ggacatcttc agatgatgct ccgaccccgg aatgacccag
     2941 agattggcct cgacatcctt gtgaaacacc atctgcccgc tgtccgggca cttgatccac
     3001 aggttctcgg gcgtctcgcg cttgccgaga aggttgcgaa tcttcgggcg gacgaggttg
     3061 gtgatccagt tcatttcgag gcctccggcc cgcagtgaaa atcaggcaag tgtccgtttc
     3121 gtggcgcgca cggccccggc cagcccttgc accagggcgg acacggccgg cacggtcgcg
     3181 ccggtcgcct ttccatccgc gtcgagggaa gcacgcaccg catccaccag cgccgagccg
     3241 acgacgacgc catcagcgcc cctggcaatg gcggcggcgt gctccggcgc cttgacgc
//
LOCUS       DAOMXN010000251         3224 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_16649_length_3224_cov_14.745661, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000251 DAOMXN010000000
VERSION     DAOMXN010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16649_length_3224_cov_14.745661"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PK812_12985"
     CDS             <1..308
                     /locus_tag="PK812_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRJ70510.1"
                     /translation="PAGPEAVRFGPFVFHPKRGDLRQGEEPVKITNRERDILRQLALA
                     LGGTVPREALTDHESSANERTIDVQINRLRRKIERDAADPVYLQTVRGLGYRLAVDP"
     gene            356..1690
                     /locus_tag="PK812_12990"
     CDS             356..1690
                     /locus_tag="PK812_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006338491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRJ70511.1"
                     /translation="MGLAYGRASIWLRRRMPKRLFARALLIIITPMVLLQSVIAYAFM
                     ERHWQLVTQRLSHALVQDIAAIIEIYRAQPRGASTEWLEEIVRAKQGLDIDFLPAEPL
                     PPALEKPFFSILDTSLSTELRRQIARPFFIDTVGRSSLLEIRVQLDDATLRVIARRSQ
                     AYASNTHIFMIWMVGTSLILIVIAVLFLRNQIKPILRLADAADSFGKGREMEFRPHGA
                     QEVRRAGVAFLEMKRRIERAMEQRTTMLNGVSHDLRTVLTRFRLSLALLDQTPEVEEL
                     KRDVDEMSKMLESYLAFARGDTSEAAAPTDIRGLLETLKSDAERSGHVTSLRIEGDPV
                     VSVRPDAFKRMLENLVSNAARFGDRIEIAAVHDDRYLTVTVDDDGPGIPPELRDQVFR
                     PFLRLDEARNQDHGGSGLGLAIVRDIARSHGGDVTLADAPMGGLRAVVRIPA"
     gene            complement(1662..1964)
                     /locus_tag="PK812_12995"
     CDS             complement(1662..1964)
                     /locus_tag="PK812_12995"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF013494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HRJ70512.1"
                     /translation="MSGTRVIMDMPEHWVRVGDHIEREFRFSSFADAIAFMVRVSFHC
                     ERMDHHPEWTNVYTRVRVALTTHDAGTVTAKDVALADVMNRVFEAFAVTPVSAPRP"
     gene            complement(1961..2443)
                     /locus_tag="PK812_13000"
     CDS             complement(1961..2443)
                     /locus_tag="PK812_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRJ70513.1"
                     /translation="MSDSSQTRTENRLITRLAHLVFRFTRPMTLGVRAIVLDGEGRVF
                     LVRHSYVPGWHLPGGGVEPGETLEAAMRKELREEGNIEVTGALALHGVFHNAHVSRRD
                     HVAVYVVRDFTQTGPRAADHEIVETGFFALDALPETTTKGTRARLTEALEGRPPAACW
                     "
     gene            2522..>3224
                     /locus_tag="PK812_13005"
     CDS             2522..>3224
                     /locus_tag="PK812_13005"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRJ70514.1"
                     /translation="MTFLFAHLSDPHVGPLPTPRWRDLAGKRLTGYWNWRTSRKTIHN
                     MEVLAAIRDDIVAHRPDHIALTGDLVNIGLAAEFPLAQRLLDGLGDGTQVSVIPGNHD
                     AYVTGSLDAMTRVFSPCMTGDDGVTGFPYLRTRGGIAFVGVSSGIPTAPFFASGAVGP
                     DQMRRLETLLADLRLRNIPVVVLIHHPPHRAGARFGRGLRDAGALEHLLARHGADLVV
                     HGHNHRFSVARLRGPA"
BASE COUNT          545 a         1153 c          998 g          528 t
ORIGIN      
        1 agccggccgg accggaagcg gtgcgcttcg gacctttcgt cttccacccc aagcgcggcg
       61 acctgcgcca gggcgaggaa ccggtgaaga tcaccaaccg cgaacgcgac atcctgcgcc
      121 agcttgccct ggcgctgggt ggcaccgtgc cgcgcgaggc cctcaccgac cacgagagct
      181 ccgccaacga acgcaccatc gacgtgcaga tcaacaggct gaggcgcaag atcgagcgtg
      241 acgccgcgga cccggtgtat cttcagaccg tgcgcggcct cggctatcgc ctggcggtcg
      301 atccttgagc gcgatcacgc ggttgcgcaa gttcgccctg cggccactgg catcgctggg
      361 actggcctac ggacgcgcca gcatctggct gcgccggcgc atgcccaagc gcctgttcgc
      421 ccgcgccctg ctgatcatca tcacgccgat ggtgctgctg cagtccgtca tcgcctacgc
      481 cttcatggaa cgccattggc aactggtgac gcagcggctg tcgcacgcac tggtgcagga
      541 tatcgcggcg atcatcgaaa tctaccgcgc acagcccagg ggcgcatcca cggaatggct
      601 ggaggagatc gtccgggcca aacaggggct cgacatcgac ttcctgcccg cagaaccttt
      661 gcctccggcg ctcgagaagc ccttcttctc gatcctcgac accagcctgt ccacggagct
      721 gcgccgccag attgcgcggc cgttcttcat cgataccgtc gggcgctcca gtctgctgga
      781 aatccgggtg cagctcgatg acgccacact gcgcgtcatc gcccggcgca gccaggccta
      841 tgcctcgaac acgcacatct tcatgatctg gatggtcggc acctccctga tcctgatcgt
      901 catcgcggtg ctgttcctgc gcaaccagat caagccgatc ctgcgcctgg cggatgccgc
      961 cgacagcttc ggcaagggac gcgagatgga gttccggccg cacggcgcgc aggaggtgcg
     1021 ccgggccggc gtggccttcc tcgaaatgaa gcggcgcatc gagcgcgcga tggaacagcg
     1081 caccaccatg ctgaacggtg tcagccacga tctgcgcacc gtgctgacgc gcttccggct
     1141 gtctctggcc ctgctcgacc agacgcccga agtggaggaa ctgaagcgcg acgtcgacga
     1201 aatgagcaag atgctcgaat cctacctggc cttcgcgcgc ggcgacacca gcgaggccgc
     1261 cgcgccgacc gacattcgcg gcctgctcga aacgctgaag agcgatgcgg aacgctccgg
     1321 ccacgtcacg agcctgcgga tcgagggtga ccccgtcgtg agcgtccgcc ccgacgcgtt
     1381 caagcgcatg cttgaaaacc tcgtgtccaa cgccgcccga ttcggcgacc gcatcgagat
     1441 cgcggcggtt catgacgacc gctatctcac ggtgacggtg gatgacgatg gtcccggcat
     1501 cccgcccgaa ctgcgcgatc aggtattccg gccgttcctg cgtctcgacg aggcccgcaa
     1561 ccaggaccat ggcggctccg gcctcggcct tgccatcgtc cgcgacatcg cccgttcgca
     1621 tggcggcgat gtgaccttgg ctgatgcgcc catgggcggg ctcagggccg tggtgcggat
     1681 accggcgtaa cggcgaacgc ctcgaacacc cggttcatca cgtcggcgag cgcaacatcc
     1741 tttgccgtca cggtgccggc atcgtgcgtc gtcaacgcga cgcggacccg agtgtagaca
     1801 ttggtccact ccggatgatg gtccatcctc tcgcaatgga acgatacccg gaccatgaag
     1861 gcgatggcat cggcgaagct cgaaaaccgg aactcccgtt cgatgtggtc gcctaccctt
     1921 acccagtgct ccggcatgtc catgatcacc ctcgtcccgc tcaccagcac gccgccggcg
     1981 ggcgcccctc cagcgcttcc gtcaggcgtg cgcgcgtgcc cttcgtcgtt gtctcaggca
     2041 gcgcgtcaag cgcgaaaaac ccggtctcga caatctcgtg atccgccgcg cgcggcccgg
     2101 tctgcgtaaa atcgcgcacc acatagaccg cgacatgatc gcgccgcgac acatgggcgt
     2161 tatggaagac gccatgcagc gccagcgcgc cggtgacctc gatattgcct tcctccctca
     2221 gttccttgcg catcgccgct tccagcgtct cgcccggctc gacaccgcca cccggcaagt
     2281 gccagcccgg cacataggaa tgacgcacga gaaagacgcg cccctcgcca tccagcacga
     2341 tggcgcgcac gccaagcgtc atcggccgag tgaagcggaa gacgagatgg gccaggcgcg
     2401 tgatcagccg gttctcggtg cgggtctgcg atgaatcgct catggcgacg gtgtatcaga
     2461 accggcgatg gctggcgcgc cggacttgac ggcggaccgc cgtcgcgaca caggcagccc
     2521 cgtgactttc cttttcgccc acctgtctga cccccatgtc ggcccgctgc cgacgccgcg
     2581 ctggcgcgac ctcgccggca agcggctgac cgggtactgg aactggcgca ccagccgcaa
     2641 gaccatccac aacatggagg tgctggcggc aatccgcgac gacatcgtcg cacatcggcc
     2701 cgatcacatc gcgctcaccg gcgatctcgt gaacatcggg cttgccgccg aattcccgtt
     2761 ggcccagcgc ctgctggacg gactgggcga cggcacgcag gtcagcgtca ttcccggcaa
     2821 ccacgacgcc tacgtgaccg gaagtctcga cgccatgaca cgggtcttca gcccctgcat
     2881 gacgggagac gacggcgtga ccggcttccc ctatctgcgg acgcgcggcg gcatcgcctt
     2941 tgtcggtgtc tcgtccggca tcccgaccgc gccattcttc gcctccggcg ccgtcggtcc
     3001 cgatcagatg cgtcggcttg aaacgcttct tgcggatctg agactgcgca acattccggt
     3061 cgtggtgctg atccaccatc cgccccaccg cgccggcgcg cggttcggac gcggactgcg
     3121 cgatgccggc gcgctggagc atctcctcgc gcgccatggc gcggacctcg tggtgcacgg
     3181 gcacaaccat cgcttctcgg tggcgcgtct gcgcggtcct gccg
//
LOCUS       DAOMXN010000252         3210 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_16767_length_3210_cov_11.496038, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000252 DAOMXN010000000
VERSION     DAOMXN010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16767_length_3210_cov_11.496038"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            372..1319
                     /locus_tag="PK812_13010"
     CDS             372..1319
                     /locus_tag="PK812_13010"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HRJ70515.1"
                     /translation="MTMSLDPMSPQRSFQGLILTLQRFWAEQGCVILQPYDMEVGAGT
                     FHPATTLRALGPKPWKAAYVQPSRRPKDGRYGENPNRLQHYYQYQVILKPSPPNLQDL
                     YLQSLYAIGLDPKLHDFRFVEDDWESPTLGAWGLGWECWCDGMEVSQFTYFQQVAGFE
                     CAPVAGELTYGLERLAMYVQGVENVYDLNFNGRDGAEKVTYGDVFLQAEQEYSRHNFE
                     HADTDMLFAQFRMAEAACQKYLAAGDPGETPNDPRHKLALPAYDQCIKASHVFNLLDA
                     RGVISVTERQSYILRVRELAKACGAAWLKTEGGGVCAPA"
     gene            1447..1686
                     /locus_tag="PK812_13015"
     CDS             1447..1686
                     /locus_tag="PK812_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HRJ70516.1"
                     /translation="MTAPVIAAKAPIAVKVEAGKDYWWCSCGKSSKQPFCDGSHKGST
                     FAPVKYTADKDGDVYFCACKNSKNGVKCDGTHKSL"
     gene            1791..2195
                     /locus_tag="PK812_13020"
     CDS             1791..2195
                     /locus_tag="PK812_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin resistance protein"
                     /protein_id="HRJ70517.1"
                     /translation="MQTARITGQASTLLTADFSRARHFWTEVAGFETVGLWGEPPDFG
                     IFSRDGAFIMVGVATKRKPPKPVFEFRPGLWNAYFWVDDARAMFAELSGRGATIDYDL
                     CEQPYGVLEFGVRDADGHDIGFGQVLPEAGHS"
     gene            2204..>3210
                     /gene="glyS"
                     /locus_tag="PK812_13025"
     CDS             2204..>3210
                     /gene="glyS"
                     /locus_tag="PK812_13025"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018260764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HRJ70518.1"
                     /translation="MPDLLLELFSEEIPARMQAKAADDLRRLVTDALVERGLVYEGAK
                     AFVTPRRLVLHVAGLPLQGRDVREEKKGPRVGAPEQAVLGFLKSAGLASLDQAKIESD
                     PKKGDFYVAVIEKPGQRTLDALAQVLPAVIRAFPWPKSMRWGAASAQPGSLRWVRPLQ
                     AIVATFGTENDEPEIVPFEVDGLAAGNVTFGHRFHAPGPIRVKRLDDHTTCLEAAKVV
                     VDPERRKAIIRTDARNLAFAQGLEVIEDEGLLEEVAGLVEWPVVLMGDFDPAFLEVPP
                     EVIRATIRANQKCFVLKDAKSGRLAPKFLLTSNIAASDGGATIVAGNGRVIRARLSDA
                     R"
BASE COUNT          561 a         1040 c         1045 g          564 t
ORIGIN      
        1 ccccgcctcc acccccgccg ccgttgtcgc gggcggctgc cggggacgac agcagggtgg
       61 aaccgacgcc gagcccgcag acggacagga acagcgcggt ggcaacgatg cgaatgttgg
      121 tcggtttcat ggtggtctcc attggatggc ccgtgatcgg gcgatgcgtg gagactgccg
      181 ttaaccactt gttaacgatg tgcgcggcct cacccgacac gtcctgcgga cgcggggctt
      241 gacccccgaa gcggcgcttg tcatggaaaa tcacggtgtt ccggtgcgct ggcgcacggg
      301 aaacggggat ttccggcgat gcctagcgaa tgcagctggc ctggttcgct ccggtctcgc
      361 cacagatggg aatgacaatg agccttgatc cgatgtcgcc gcagcgctcc ttccaggggc
      421 tgatcctgac gctccagcgc ttctgggcgg aacagggctg tgtcatcctc cagccttacg
      481 acatggaagt cggcgccggc accttccatc ccgccacgac gttgcgcgcg ctggggccga
      541 agccgtggaa ggcggcctac gtgcagccct cgcgccggcc gaaggacgga cgctatggcg
      601 agaacccgaa ccgtctccag cactattacc agtatcaggt gattctgaag ccttcgccgc
      661 cgaacctgca ggacctctac ctgcaatcgc tctacgccat cggactggat ccgaaactgc
      721 atgacttccg cttcgtcgag gacgattggg aaagcccgac tctcggcgcc tgggggctcg
      781 gctgggaatg ctggtgcgac ggcatggagg tctcgcagtt cacttacttt cagcaggtcg
      841 ccggcttcga atgcgcgccg gtcgccgggg agcttaccta tgggcttgag cgcctcgcca
      901 tgtatgtgca gggcgtcgag aacgtctacg acctcaattt caacggacgg gacggcgcgg
      961 aaaaggtcac ctacggcgat gttttcctgc aggccgagca ggagtattcg cgccacaatt
     1021 tcgagcatgc cgacaccgac atgctgttcg cgcagttccg catggcggaa gcggcctgcc
     1081 agaagtatct cgctgccggc gaccccggcg agacccccaa cgatccgcgc cacaagctcg
     1141 cgctgccggc ctatgaccag tgcatcaagg cgagccacgt cttcaacctg ctggatgcgc
     1201 gcggcgtgat ctcggtcacc gaacgccaga gctacattct gcgcgtgcgt gagctggcga
     1261 aagcctgcgg cgcggcctgg ctgaagacgg aaggcggcgg cgtatgcgcc ccggcatgat
     1321 ccggtcttgc attccggcaa ggaagttttt ggattttccc acgaaacctg cactggcgca
     1381 aaaatcgaac cgttctaaat tgtgcaggaa gccggatgtt cggcgcccct cacctggaga
     1441 ccacgcatga ccgctcccgt tatcgccgca aaagccccga tcgccgtcaa ggtcgaagcc
     1501 ggcaaggact actggtggtg ttcctgcggc aaatcgtcga agcagccgtt ctgcgacggc
     1561 tcgcacaagg gttcgacttt cgcgccagtg aaatacaccg ccgacaagga cggcgacgtc
     1621 tatttctgcg cctgcaagaa ttccaagaac ggcgtgaagt gcgacggcac gcacaagtcg
     1681 ctctgatttg ccgaacggcg cgggcttttc cgcgcgctgc ttctggacac gacccgcgcg
     1741 gcgaattacc acgcccgcgg gtttttcttt gagcgcggga gcggcgacag gtgcagacag
     1801 cgcggatcac cggccaggcc agcacgttgc tgaccgccga tttctcgcgc gcccggcact
     1861 tctggacaga agtcgccggc ttcgagaccg tcggactctg gggagagccg ccggatttcg
     1921 gcatcttctc gcgcgatggc gcgttcatca tggtcggcgt tgcaacaaaa cggaagccgc
     1981 cgaaaccggt gttcgagttc cgtcccggct tgtggaacgc ctatttctgg gtggacgacg
     2041 ctcgcgcgat gttcgcggaa ctgtccggcc gcggcgcgac gattgactac gatctgtgtg
     2101 agcagccgta tggcgtgctt gaattcggcg ttcgcgatgc ggatggccac gacatcggct
     2161 tcgggcaggt gctgcctgaa gcgggacact cctgaggcga ccgatgcccg atctgctgct
     2221 cgaactcttt tccgaggaaa tcccggcccg catgcaggcg aaggccgcag acgacctgcg
     2281 gcggctggtg accgacgccc tggtcgagcg cggcctcgtc tatgaaggcg ccaaggcgtt
     2341 cgtcactccg cgccgcctcg tgctgcatgt tgccggcctg ccgctgcagg gccgcgacgt
     2401 gcgcgaggag aagaagggcc cccgcgtcgg cgcgccggag caggcagtgc tcggctttct
     2461 caagagcgca ggacttgcgt ccctcgacca ggcgaaaatc gaaagcgacc cgaagaaggg
     2521 cgatttctac gttgccgtca tcgagaagcc gggccagcgc acgctcgatg cgctggcgca
     2581 ggtgctgccg gcggtgatcc gcgcctttcc ctggccgaaa tcgatgcgct ggggcgcggc
     2641 ttccgcgcag cccggctcgc tgcgctgggt gcgcccgttg caggccatcg tcgcgacatt
     2701 cggcacggag aacgacgagc ccgagatcgt tcccttcgag gtggacgggc tggccgccgg
     2761 caatgtcacc ttcggccacc gtttccatgc gcccggcccc atccgggtga agcggctcga
     2821 cgaccatacg acatgtctcg aagcggcgaa ggtcgtggtc gatccggagc ggcgcaaggc
     2881 gatcatccgc acggacgccc gcaatctcgc cttcgcgcag ggccttgagg tgatcgagga
     2941 cgagggcctg ctggaggaag tcgccggcct cgtcgaatgg cccgtggtgc tgatgggcga
     3001 tttcgatcca gccttcctcg aagtgccgcc ggaagtgatc cgcgccacca tccgcgccaa
     3061 ccagaaatgc ttcgtcctca aggatgcgaa gtccggccgg ctcgcgccga aattcctgct
     3121 cacctcgaac atcgcggcca gtgacggcgg ggcgaccatc gtcgccggca acggccgggt
     3181 catccgcgcc cgactgtccg acgcccgctt
//
LOCUS       DAOMXN010000253         3202 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_16834_length_3202_cov_12.878932, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000253 DAOMXN010000000
VERSION     DAOMXN010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16834_length_3202_cov_12.878932"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..2335
                     /locus_tag="PK812_13030"
     CDS             182..2335
                     /locus_tag="PK812_13030"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HRJ70519.1"
                     /translation="MPEPVPPAASDTLRTSPSRFVNREISWLSFNRRVLEEASNESHP
                     LLEQLRFLSISASNLDEFFMVRVAGLVEQVINNVQQASDDGLSPAEQLVRINEAVAAL
                     TADQQKRWSQVRALLSRKKIRIVDTSELTDAEKHWLNDYFLASVFPTLTPLAIDPAHP
                     FPFIPNLGFSLALSLSRRSDGRQLRALVRMPQKVDRFIRLPSPEGMNRFVVLEQAILL
                     FIKKLFPGYKVLGQGCFRLIRDSDVEIEEEAEDLVRVFERMLKRRRRGVVIRLELDAA
                     MPEELRNFVVQELKVTHGEVALIDGVIALSDLSQIVSVDRPDLKFGPFEPRFPERIRE
                     QGGDSFVAIRQKDLVVHHPYESFDSVVQFLAQAARDPDVVAIKQTLYRTSADSPIVRA
                     LAEAAEAGKSVTALVELKARFDEEANIRWARDLERAGVQVVYGFIELKTHAKLSMVVR
                     REAGQLVTYCHIGSGNYHPITAKIYTDLSFFTADRVIGRDVARIFNFITGYAEPAELE
                     MLSVSPLTLKKRILKHIADEIEHVRQGRPGAIWLKCNSLVDPMIIDALYDASAAGVKV
                     DLVVRGICCLRPGVPGLSENIRVKSIVGRFLEHARIYAFGCGFGLPHPKARVYVSSAD
                     LMPRNLDRRVEVLLPLTNPTVHEQVLEQIMYANLLDNQQSWRVLQDGSAVRIRPEPGE
                     EPFNAHKYFMTNPSLSGRGKSLKVSSPRDLLRSRG"
     gene            2349..2978
                     /gene="purN"
                     /locus_tag="PK812_13035"
     CDS             2349..2978
                     /gene="purN"
                     /locus_tag="PK812_13035"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HRJ70520.1"
                     /translation="MLISGRGSNMAALVEAARAPDYPAQIVLVLSNKADAAGLGFAED
                     AGIATAVVSHRGFRDREAYDRLVHERLLAAGAEFVCLAGFMRMLSPWFCQQWAGRMIN
                     VHPSLLPSFRGLDTHARALAEGVKLHGASVHFVVPELDAGPIIAQAAVPVLDDDTEQT
                     LAARVLAQEHVIYPLALKLAASGALRIEGNRVLGGVSAVSSAHPSSPRD"
BASE COUNT          532 a         1071 c         1008 g          591 t
ORIGIN      
        1 atcacgcggc cggtggccgc cgccgttctg gagcggttgt ccggctgcgc ggggtaggcc
       61 cgtttgcgct gcgccattgc ggccttgtca tgctgctgtc attttcttgg gcgaggagac
      121 agtccagatc atggaaacgc ccgcaaaacc gacgcgcgcg cgcgcagcgc gctccagaaa
      181 aatgcccgag cccgtcccgc ccgccgcgag cgatacgctg cgcacctcgc cgtcccgctt
      241 tgtgaatcgc gagatttcct ggctgagctt caaccgccgc gtgttggagg aggcatcgaa
      301 cgagagccat ccgctgctgg aacagctgcg cttcctgtcg atttcggcca gcaacctcga
      361 cgagttcttc atggtgcgtg tcgccgggct cgtcgaacag gttatcaaca acgtccagca
      421 ggcgtccgat gacgggcttt caccggccga gcaactggtg cgcatcaacg aggcggtggc
      481 ggcgcttacc gcagaccagc agaagcgctg gtcgcaggtg cgagcgctgc tctccaggaa
      541 aaagatccgc atcgtcgaca cctcggaact gacagacgcg gagaagcact ggctcaacga
      601 ctatttcctg gcctcggtct tcccgacgct gacaccgctt gccatcgatc cggcccatcc
      661 cttccccttc atcccgaacc tcgggttctc gctggcgctg tcgctgtcgc ggcgttcgga
      721 cggccgacag ttgcgcgcgc tcgtgcgcat gccgcagaaa gtcgaccgct ttatccgctt
      781 gccttcgcct gaaggcatga accggttcgt cgtgctggag caggcgatac ttctcttcat
      841 caagaagctc tttcccggct acaaggtgct tggtcagggc tgcttccgcc tgatccgcga
      901 ctccgacgtc gagatcgagg aagaagcgga ggacctcgtg cgcgtgttcg agcgcatgct
      961 gaagcgccgg cgccgcggtg tcgtcattcg cctcgaactc gacgccgcga tgcctgagga
     1021 actgcgcaac ttcgtcgtgc aggaactgaa ggtcacccac ggcgaggtgg cgctgattga
     1081 cggcgtgatc gccctgtccg atctgtccca gatcgtcagc gtcgaccgtc cggacctgaa
     1141 attcgggccg ttcgaacccc gctttccgga acgtatccgc gaacagggcg gcgacagttt
     1201 cgtggcgatc cgccagaagg accttgtcgt ccaccatccc tacgagtcct ttgactcggt
     1261 tgtgcagttc ctggcgcagg cggcgcgcga tcccgacgtg gtggcgataa agcagacgct
     1321 ttatcgcacc tcggcggaca gccccatcgt gcgcgcgctg gcggaagcgg cggaggccgg
     1381 caagtcggtg acggcgcttg tggaactgaa ggcgcggttc gacgaagaag cgaacatccg
     1441 ctgggcccgc gatctggagc gcgccggcgt gcaggtcgtc tatggcttca tcgaactcaa
     1501 gacgcacgcc aagctttcga tggtggtgcg ccgcgaggcc gggcagctcg tgacctattg
     1561 ccacatcggc tcgggcaact accatccgat caccgccaag atttacaccg acctgtcctt
     1621 cttcaccgca gatcgggtga tcgggcgcga cgtggcgcgc atcttcaatt tcatcacggg
     1681 ctacgccgag ccggccgagc tggaaatgct ctccgtctcg ccgctcacgc tgaagaagcg
     1741 catcctcaag catatcgccg acgagatcga acatgtgcgc cagggccgtc ccggcgcgat
     1801 ctggctgaaa tgcaactcgc ttgtcgaccc gatgatcatc gacgcgctgt atgacgcaag
     1861 cgcggccggc gtgaaggtcg atctcgtggt gcgcggcatc tgctgcctgc gtcccggcgt
     1921 gcccggcctg tcggagaaca tccgcgtcaa gtcgattgtc gggcgcttcc tcgaacacgc
     1981 gcgcatctat gccttcggct gcgggttcgg gctgccccac cccaaggcac gggtttatgt
     2041 gtcctcggcc gatctgatgc cccgcaatct cgaccgccgc gtcgaggtgc tgctgccgct
     2101 gaccaacccg accgtgcacg aacaggttct tgagcagatc atgtacgcca accttctcga
     2161 caaccagcag agctggaggg tcttgcagga tgggtccgcc gtgcgcataa ggccggagcc
     2221 gggcgaagag ccgttcaacg cccacaagta cttcatgacc aatccgagcc tctccggacg
     2281 tggaaaatcc ctcaaggtct ccagcccccg cgacctcctg cgcagcaggg gctaggaaac
     2341 gcgtcgccat cctcatttcc gggcgcggct ccaacatggc cgcgctggtc gaggcggcaa
     2401 gggcgccgga ctacccggcg cagatcgtgc tggtcctctc gaacaaggct gacgccgccg
     2461 gccttggctt cgccgaagat gccggaatcg ccaccgccgt cgtctcccac cgcgggtttc
     2521 gcgaccgcga agcctatgac cggctcgtgc atgagcggct cctggccgcc ggcgcggagt
     2581 tcgtctgtct cgccggtttc atgcgcatgc tctcgccctg gttttgccag cagtgggccg
     2641 gccggatgat caatgtgcat ccttcgctgt tgccgagttt ccggggcctc gacacacacg
     2701 cccgcgcatt ggcggagggc gtgaagctgc atggggcgag cgtgcatttc gtcgtgccgg
     2761 aactggacgc cggtccgatc atcgcgcagg ctgcggttcc ggttctggat gacgacacgg
     2821 agcagacgct cgccgcccgc gtgctcgccc aggagcacgt catctatcct cttgccctga
     2881 agctggcggc ctccggcgcg ctcaggatcg agggaaaccg ggttctcggc ggggtgagtg
     2941 cggtatcaag cgcgcatcct tccagccctc gggactgacc ggcactatcc catttgcctg
     3001 cgccatgggc gtcccacagc cggtgtcatg ccgaggccac ttggagcaag acccatccgc
     3061 atgattccgg ggcgctccgc aggagcgagc ccggaatcca ccttccttct acatgtccgc
     3121 tggtcagatc gattcctggc gctggccaga ctcgccccgg agcggcaccg ggaggtcgcc
     3181 cgcggcccgt cagcggggcg ag
//
LOCUS       DAOMXN010000254         3108 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_17573_length_3108_cov_9.086145, whole genome shotgun sequence.
ACCESSION   DAOMXN010000254 DAOMXN010000000
VERSION     DAOMXN010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17573_length_3108_cov_9.086145"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /locus_tag="PK812_13040"
     CDS             complement(<1..922)
                     /locus_tag="PK812_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HRJ70521.1"
                     /translation="MSDKNREWSRRTRAAQALGTIDERTRAVVPPIHVATTFVRDPDN
                     QYRSGFCYARPDNATVREAEAIIASLEEAAAAIVLGSGMSAATAVFLALKPGDHIVAP
                     KVMYWSLRNWLLSYAAEWGLTTTFVDAGDLDALRGAVEPGRTRLIWLETPANPLWTIS
                     DIAGAAQIARAAGARLAVDSTAATPLLTRPLALGADIVMHSATKYLNGHSDVIAGALA
                     CARVDDFWERILKIRAAQGMILGPLEAFLLIRGMRTLDLRVRAACESAMQLATRFANH
                     ERVAQVLYPGLPSHAGHAVAQADGQHPAEAA"
     gene            1030..2217
                     /locus_tag="PK812_13045"
     CDS             1030..2217
                     /locus_tag="PK812_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HRJ70522.1"
                     /translation="MQDTGSTRDDPALARLADEVTGWRRHLHAHPELSYDVHETARFV
                     AAKLRAFGLDDVVEGIGRTGVVGVIRGRTPGRTVGLRADMDALPVQEMTNLPHASRIE
                     GRMHACGHDGHTAMLLGAARRLADRRDFSGVAVVIFQPAEEAGNGALAMVEDGVMARF
                     GIDEVYGMHNLPGLPAGAFAVARGPAMAAVDSFTIRIEGRGCHAAQPNAGIDPILAGA
                     HVVKALQAIVARNVDPLHAVVVSVTAFQGGEAFNVVPQSVLLRGTVRSMSPAVRDFVE
                     GRVSATARAVAASFGATAEVHYERLVDATVNSPAHAGHAVMAARSVPAPVPVDEDPAP
                     MMAGEDFSFMLGARPGAFVFIGNGETAGLHHPGYDFNDAIIPAGIRFWTELVHRRGKQ
                     DAD"
     gene            2221..>3108
                     /locus_tag="PK812_13050"
     CDS             2221..>3108
                     /locus_tag="PK812_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HRJ70523.1"
                     /translation="MHPAFGVNPHVAAIDAPPIPTAQAWKAAYDGRQGPMIDLSQAVP
                     GLPPPAEFLDRLAAAAGSADAARYGPILGDPDLREALAADTARTYRGAVGADNVAITS
                     GCNQAFVVAMLALAQAGDSVILPAPWYFNHRMTLDMLGIATRILPCRIEDGFVPYPEA
                     AERLIDASTRAIVLVSPNNPTGAVYPQDIIARFADLARRRGIALVLDETYRDFLPADQ
                     PRAHALFEDTHWGATLVQLYSFSKSHAIPGHRLGAIIAAPAFIAQVAKIQDSLQICPA
                     RPAQRVAAWAVDALRPWREA"
BASE COUNT          461 a         1075 c         1062 g          510 t
ORIGIN      
        1 gggcggcttc ggcgggatgc tgtccatccg cctgcgcgac ggcgtggccg gcatgggacg
       61 gcaggccggg atagaggacc tgcgccactc gttcatggtt cgcgaagcgc gtcgccaact
      121 gcatggcgct ttcgcaggcg gcgcggaccc gcaggtccag cgtgcgcatg ccgcgtatga
      181 gcaggaaggc ctcgagcggc ccgagaatca tcccctgcgc cgcgcggatc ttcaggatgc
      241 gctcccagaa atcatcgacc cgggcgcagg ccagcgcgcc ggcgatgaca tcggaatgcc
      301 cgttcaggta cttggtcgcc gaatgcatga cgatgtccgc gccaagcgcg agcggacgcg
      361 tcaacagcgg cgtggcggcg gtcgaatcca ccgcaagccg cgcgccggcg gcacgggcga
      421 tctgcgccgc accggcgata tcggagatgg tccagagcgg gttcgccggc gtttccagcc
      481 agatcagccg cgtgcgtcca ggttcgacgg cgccgcgcag cgcatcgagg tcgccggcat
      541 cgacaaaagt tgtcgtcagc ccccactccg ccgcatagct gagcagccag ttgcgcagcg
      601 accagtacat gaccttcggc gcaacgatgt ggtcgccggg tttcagcgcc aggaaaaccg
      661 ccgtcgcggc cgacatgccg gagccaagca cgatggccgc tgccgcctcc tccagcgagg
      721 cgatgatcgc ctcggcttcg cgcaccgtcg cattgtccgg gcgcgcatag cagaatccgg
      781 agcggtattg gttgtcgggg tcgcgcacga aggttgtcgc cacatggatc ggcggcacga
      841 cagcgcgggt ccgttcgtcg atggtgccca aagcctgcgc ggcgcgcgtg cgccggctcc
      901 attccctgtt cttgtcggac acgtccgtta ccccctggcc aatgaccctg ccggcagggc
      961 ggtgatgtaa cgggtcaagc tcgcggaagt cgatggcttt ctttcggcgc gtgggtatcc
     1021 tgtgacgcga tgcaagacac aggctccaca agggacgatc cggcactcgc ccggctcgcc
     1081 gatgaagtga cgggatggcg acgccatctc catgcccatc ccgaactcag ctacgacgtg
     1141 cacgagacgg cgcgcttcgt cgccgcgaag ctgcgcgcct ttggcctcga tgacgtggtc
     1201 gaaggcatag gccgcaccgg cgtcgtcggc gttatccggg ggcgcacgcc ggggcgtacg
     1261 gtcggcctca gggcggacat ggatgccctg ccggtgcagg agatgaccaa cctgccccat
     1321 gcctcgcgca tcgaggggcg catgcatgcc tgcggccacg acggacacac ggccatgctg
     1381 ctgggcgcgg ccaggcgcct ggccgaccgg cgtgattttt ccggcgttgc cgtggtgatc
     1441 ttccagccgg cggaagaggc cggcaacggc gcgctggcca tggtcgagga cggggtgatg
     1501 gcgcgcttcg gcatcgatga ggtctatggc atgcacaacc tgccgggcct gccggcggga
     1561 gccttcgccg tggcgcgcgg tccggccatg gcggcggtgg acagtttcac gatccgcatc
     1621 gagggccggg gctgccatgc ggcgcagccg aatgccggga tcgatccgat tctggcgggc
     1681 gcgcacgtgg tcaaggcgct gcaggccatc gtggcgcgca atgtcgatcc gctgcatgcg
     1741 gtcgtcgtct ccgtcaccgc cttccagggc ggggaagcgt tcaatgtcgt gccgcaatcg
     1801 gtgctgctgc gcggcacggt ccgctcgatg agcccggctg tccgggattt cgtcgaaggc
     1861 cgggtgagcg cgacggcgcg cgccgtcgcc gcttctttcg gagccactgc cgaggtgcac
     1921 tacgagcggc tggtggacgc cactgtgaac agcccggcgc atgccggcca cgccgtgatg
     1981 gcggcgcggt ccgtgccggc ccccgtgcct gtggacgagg accccgcgcc catgatggcc
     2041 ggcgaggact tcagcttcat gctcggtgcc cggcccggtg ccttcgtgtt catcggcaat
     2101 ggcgagacgg ccgggcttca tcaccccggc tacgatttca acgacgcgat catccccgcc
     2161 ggcattcggt tctggaccga attggtgcat cgccggggca agcaggacgc ggactgaacc
     2221 atgcatcccg cgtttggcgt caacccacac gtcgcggcca ttgatgcgcc gccgatcccg
     2281 accgcccagg cctggaaggc ggcctacgac ggccggcagg gtccgatgat tgacctctcg
     2341 caggccgtcc cgggcctgcc gccgccggcc gaattcctcg atcgcctggc cgctgcggcg
     2401 ggcagcgccg atgcagcgcg ctatggtccg atccttggcg atccggacct gcgagaggct
     2461 ctggccgcag atacggcccg gacctaccgg ggtgcggtcg gcgccgacaa tgtggcgatc
     2521 acctcaggct gcaaccaggc ctttgtggtt gccatgctcg cgctggctca ggccggcgac
     2581 tccgtgatcc tgcccgcgcc ttggtacttc aatcaccgca tgacgctcga catgctgggc
     2641 atcgccacgc gcatcctgcc ctgccgcatc gaggatggct tcgttcccta tccggaagcg
     2701 gccgaacgcc tcatcgacgc gagcacccgg gccatcgtgc tggtgtcgcc caacaatccc
     2761 accggcgcgg tctatcctca agacatcatc gcccggtttg ccgatctcgc gcgtcgccgg
     2821 ggcatcgccc tggtgctgga cgagacctat cgcgatttcc tgcccgctga tcagccgcgc
     2881 gcccatgccc tgttcgagga cacgcattgg ggcgcgacgc tggtgcagct ctattccttc
     2941 tccaagtcgc atgcgattcc cgggcaccgg ctcggcgcca tcatcgccgc tcccgctttc
     3001 atcgcgcagg tggccaagat tcaggacagc ctgcagatct gccccgcccg cccggcgcag
     3061 cgcgtcgcag cctgggctgt cgacgccttg cggccctggc gcgaggca
//
LOCUS       DAOMXN010000255         3100 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_17632_length_3100_cov_12.661412, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000255 DAOMXN010000000
VERSION     DAOMXN010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17632_length_3100_cov_12.661412"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..1556
                     /gene="gltX"
                     /locus_tag="PK812_13055"
     CDS             123..1556
                     /gene="gltX"
                     /locus_tag="PK812_13055"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HRJ70524.1"
                     /translation="MSTPVVTRFAPSPTGFLHIGGGRTALFNWLFARRHGGKMLLRIE
                     DTDRERSTEAAIQAILDGLSWLGVGWDGDVVYQYARAARHREVAESLVAAGKAYYCFA
                     TPQELDEMREKARAEGRPMRYDGRWRDRDPRGAPAGLKPVIRLKAPLEGETSVDDMVQ
                     GRVTWANKDLDDLVLLRSDGNPTYMLAVVVDDHDMGVTHIIRGDDHLTNAARQTQIYQ
                     ALDWAVPAMAHIPLIHGPDGAKLSKRHGALGVEAYRAMGYLPEALRNYLVRLGWSHGD
                     QEIFSTAEMTALFDLKNVGRSPARFDYAKLENINGHYMRQASDADLLARFEDVLEVHP
                     DGAALRPKLDVAMRSKLLAAMPGLKERAKTVVELIDGARFLFASRPLALDDKARQNLD
                     QSGRERLALLLPALAAVGDWSAPALEAAVRTVSEQTGAKLGHLAQPLRAALTGRATSP
                     GIFDVLAVLGREESLARIADHAASTKS"
     gene            1682..2974
                     /gene="gltA"
                     /locus_tag="PK812_13060"
     CDS             1682..2974
                     /gene="gltA"
                     /locus_tag="PK812_13060"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HRJ70525.1"
                     /translation="MTATKGTITVGDKSVEMPVRGGTIGPDVVDIAALYGKTGMFTYD
                     PGFTSTAACESQITYIDGDAGVLLHRGYPIEQLAEHGDFLETCYLLLHGELPTPAQKV
                     DFVNRVTRHTMVHEQMSRFLQGFRRDAHPMAVMVGSVGALSAFYHDSTDISDPYQRMV
                     ASMRMIAKMPTLASMAYKYSIGHPFVYPKNDLDYTSNFLRMCFAVPCEDYKVNPVLAR
                     ALDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGVACLWGPAHGGANEAALKML
                     AEIGTPDRIPHFIARAKDKNDPFRLMGFGHRVYKNYDPRARIMQRTTHEVLSEVGVKD
                     DPLLQVAMELERIALHDEYFIERKLYPNVDFYSGITLKAMGFPTSMFTVLFAVARTVG
                     WIAQWKEMIEDPNQRIGRPRQLYTGAPKRDYVPIAQRR"
BASE COUNT          552 a         1023 c          983 g          542 t
ORIGIN      
        1 agccgcgccg acggttctgg cggggatcgg agccggccaa gcgcgtctcc tgttgtgcct
       61 gtgcacggtc atgctacacg ggccgcctgt tttcgaaagc ccacaccccc ggattgccgt
      121 tgatgtccac gcccgtcgtc acccgcttcg ccccttcgcc gaccggattc ctgcacatcg
      181 gcggcgggcg caccgcgctg ttcaactggc tctttgcgcg ccgtcacggc ggcaagatgc
      241 tgttgcgcat cgaagacacc gaccgcgaac gctccacgga agcggcgatc caggccattc
      301 tcgacgggct gagctggctt ggcgtcggct gggacggcga tgtcgtctat cagtatgcgc
      361 gcgcggcgcg ccatcgtgag gtcgcggaaa gcctcgttgc ggccggcaag gcctattatt
      421 gcttcgcgac gccgcaggaa ctggatgaga tgcgggaaaa ggcgcgggcc gagggccgcc
      481 ccatgcgcta cgacgggcgc tggcgcgacc gggaccccag gggagcgccg gccggattga
      541 agccggtgat ccggctcaag gcgccactgg agggggaaac cagcgtcgat gacatggtgc
      601 agggccgcgt cacctgggcc aacaaggacc tcgacgatct ggtcctgctg cgctccgacg
      661 gcaacccgac atacatgctg gccgtggtcg tcgacgatca cgacatgggc gtcacgcaca
      721 tcatccgggg cgacgatcac ctgaccaacg ccgcgcggca gacccagatc taccaggctc
      781 tggactgggc tgtaccggcc atggcgcata ttccgcttat ccacggaccg gacggggcga
      841 aactgtcgaa gcgccatggt gcgctcggcg tcgaggccta tcgcgccatg ggctacctgc
      901 cggaagcgct gcgcaactac ctcgtgcgcc tgggctggag ccacggcgat caggagattt
      961 tctccaccgc cgagatgacc gccctgttcg acctgaagaa cgttggtcgc tcgccggcgc
     1021 gtttcgatta cgccaagctt gaaaacatca atggccatta catgcgccaa gccagcgatg
     1081 cggacctgct ggcccggttc gaggatgtgc tggaggtgca cccggatggg gcggcactgc
     1141 gtccgaaact cgacgttgcc atgcgatcca agcttctggc cgccatgccc ggcttgaaag
     1201 aacgcgcgaa gaccgtggtg gaactcattg acggcgctcg ctttctcttc gccagccgcc
     1261 cgctcgccct ggacgacaag gcgcgccaga acctggacca gtccgggagg gaacggttgg
     1321 cgctgctcct gccggctctc gccgcggtgg gcgactggtc ggcccccgcg ctggaagcgg
     1381 cggttcgcac cgtctccgaa cagaccgggg cgaaacttgg ccatctcgcg cagcccctgc
     1441 gcgccgcgct gacaggccgc gctacctctc cgggcatatt tgacgtgctg gccgtgctgg
     1501 ggcgtgagga aagcctcgcg cgcatcgccg accatgcggc ttcgaccaag agctaagctc
     1561 cggattagca aaccgcacgc catattgaaa tgcggcaact tgaaggcttt gctgcattgc
     1621 ggtaggcaag ggtacacacg ttgacagaca tgcggcgcat ggcgccgtcc ggaggttcaa
     1681 gatgaccgct acaaagggca ccattacggt tggcgacaag tcggtggaaa tgccggtgcg
     1741 cggcggcacg atcggcccgg acgtggtgga tatcgccgcg ctctacggca agaccgggat
     1801 gttcacctac gatcccggct tcacctcgac cgccgcctgc gaaagccaga tcacctatat
     1861 cgacggcgat gccggtgtat tgctgcaccg cggctatccg atcgaacagc tggcggagca
     1921 tggcgacttc ctggagacct gctacctgct gctgcacggc gagttgccga ccccggccca
     1981 gaaggtcgat ttcgtcaatc gcgtcacgcg ccacaccatg gtgcacgagc agatgagccg
     2041 gttcctgcag ggcttccgcc gcgacgccca cccgatggcg gtgatggtcg gttcggtcgg
     2101 cgcgctgtca gccttctatc acgactcgac agacatttcc gacccgtacc agcgcatggt
     2161 cgcctccatg cgcatgatcg ccaagatgcc gacgttggcg tcgatggcct acaagtactc
     2221 gatcggtcat cccttcgtct atccgaagaa cgacctggac tacacgtcca atttcctgcg
     2281 catgtgtttc gcggttccct gcgaggacta caaggtgaac ccggttctgg cgcgcgcgct
     2341 ggaccgcatt ttcatcctgc atgctgatca cgagcagaac gcctcgacgt ccaccgtgcg
     2401 tcttgcgggg tcctcgggcg cgaacccgtt tgcctgcatc gcggccggcg tcgcctgcct
     2461 ctggggcccg gcccacggcg gcgcgaatga agcggcgctg aagatgctgg cggaaatcgg
     2521 cacgccggac cgcatcccgc acttcatcgc gcgcgcaaag gacaagaacg atcccttccg
     2581 tctcatgggc ttcggccacc gcgtctacaa gaattacgat ccgcgcgcgc ggatcatgca
     2641 gcgcaccacg cacgaggttc tttccgaagt cggcgtgaag gatgatcccc tgctccaggt
     2701 cgcgatggaa ctggagcgca ttgcgctgca cgatgagtat ttcatcgagc gcaagctcta
     2761 cccgaacgtc gacttctatt ccggcatcac gctgaaggcg atgggcttcc ccacctcgat
     2821 gttcacggtg ctcttcgccg tggcgcgcac ggtcggctgg attgcccagt ggaaggagat
     2881 gatcgaggac ccgaaccagc gcatcggtcg tccgcgccag ctctacaccg gcgcgccgaa
     2941 gcgcgactac gtgccgatcg cccagcggcg ctgagtcagg cacgcctgca cgaaagaagg
     3001 cgcctgcggg cgcctttttt gttcgccgca gcgacctggc caggaaacga cgatctgata
     3061 ccatcggtca tcagggatga ccgatgaccg ctcttgaatt
//
LOCUS       DAOMXN010000256         3098 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_17647_length_3098_cov_10.234965, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000256 DAOMXN010000000
VERSION     DAOMXN010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17647_length_3098_cov_10.234965"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="PK812_13065"
     CDS             complement(<1..374)
                     /locus_tag="PK812_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70526.1"
                     /translation="MVASRLAFGLAGLLMASTAVKAQFILPQDYRQPNTARVQYAPVD
                     RALQPTAPSAHTDNRAPTQQPQAFFPQNFRADKPITQGESRTNWQDHRNQAESRQDVR
                     APVELQPRAAARIRLHRLAAPQP"
     gene            complement(565..1449)
                     /gene="mmsB"
                     /locus_tag="PK812_13070"
     CDS             complement(565..1449)
                     /gene="mmsB"
                     /locus_tag="PK812_13070"
                     /EC_number="1.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008442 - 3-hydroxyisobutyrate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyisobutyrate dehydrogenase"
                     /protein_id="HRJ70527.1"
                     /translation="MTTIAFIGLGNMGGPMAANLVKAGHAVTGFDLSEASKKAAAEAG
                     VAVAADARAAVKAADIVVTMLPAGKHVRAVWADLIPAAKKSALFIDSSTIDVDSARAV
                     HKLAAEAGMQSVDAPVSGGTGGAKAATLTFMCGGEKGAFERAQPVLAAMGKKIVHCGG
                     AGAGQAAKICNNMILGISMIAVAEGFVLAEKLGLSHQALFDVASTSSGQCWSLTTYCP
                     VPGPVPTSPANNGYKPGFAAELMLKDLKLAQEAAISSGATTALGAAATQLYSLFNAAG
                     EGGADFSGIITMIRGRQG"
     gene            complement(1499..2635)
                     /locus_tag="PK812_13075"
     CDS             complement(1499..2635)
                     /locus_tag="PK812_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRJ70528.1"
                     /translation="MTYALTEDQIAIRDMARAFADERIAPNALKWDEEKHFPVDVMRE
                     AAALGMGGIYVREDVGGSGLSRLDAALIMEALATGCPAVSAFISIHNMAAWMIDRYGD
                     EALRQRFLPKLVTMESIASYCLTEPGAGSDAAALKTKAERDGDVYVLNGTKQFISGAG
                     SSDAYVTMVRTGGPGAGGISCLVVENGAPGLSFGANEKKMGWNAQPTRQVIFENCRVP
                     VANRVGEEGLGFKIAMAGLDGGRLNIGACSLGGAQAALEKSVRYASERKAFGKALNEF
                     QALQFKLADMATGLEAARVMLYAAAAAYDRQDPETTMRCAMAKRFASDTGSQVVDEAL
                     QIHGGYGYLHEYGIEKLSRDLRVHRILEGTNEIMRLIVARKMMG"
     gene            complement(2640..3068)
                     /locus_tag="PK812_13080"
     CDS             complement(2640..3068)
                     /locus_tag="PK812_13080"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRJ70529.1"
                     /translation="MDRAETFDLEAAKKLLGEVFAPWIQDLKLEVMSVARDEVVLRMP
                     FSNRLTRSGGIICGQALMALADTAMVFAVAAASGRSRPMTTVDQTTHFLRPVSSSAVL
                     CAVRITRLGRTMAFAQATISTESDGKPVATAQLAYALLEG"
BASE COUNT          505 a         1039 c         1046 g          508 t
ORIGIN      
        1 ggctgtggcg cggcgaggcg gtggagacgg atgcgggcag cggcgcgcgg ctggagctcg
       61 acaggcgcgc gcacgtcctg acgcgattcg gcctgattgc ggtgatcctg ccagttggtc
      121 cgggactcgc cctgggtgat gggcttgtcg gcgcggaaat tctgcgggaa aaacgcctgc
      181 ggctgctggg tgggcgcccg gttgtccgtg tgcgcagagg gtgccgtcgg ctgcagcgcg
      241 cgatcgaccg gcgcatactg cacgcgcgcc gtgttcggct ggcgatagtc ctgcggcaga
      301 atgaactggg ccttcaccgc cgtggaggcc atcagcaggc cagccaaccc gaatgcaaga
      361 cgagacgcaa ccatggtcaa ctccgaagaa tccgcgcgta gcaaaggcct ccgcgcggct
      421 ttcgggtcac cctgcgtgag aatgctttgc gcaaggtaaa gccaagcgct gccgctgcgg
      481 cagcgcctgt gaaccgtcag gtagggttaa gggatcgaca accgggacgg gagtcggctg
      541 tgcctcgcgg acacagcgcc gggttcagcc ctggcggccc cggatcatgg tgatgatgcc
      601 ggagaaatcc gccccgccct cgcccgcggc attgaacagc gaatagagct gcgtcgccgc
      661 cgcgcccagc gcggtggtcg cgccagagga aatcgccgcc tcctgcgcca gcttgagatc
      721 cttcagcatc agttcggcgg caaatcccgg cttgtagcca ttgttggccg ggctggtcgg
      781 caccggtccg ggcaccgggc aataggtggt caggctccag cactggccgg aggaggtgga
      841 ggcgacatcg aacagcgcct gatgcgacag gccgagtttc tccgcgagca cgaagccttc
      901 cgccaccgcg atcatcgaaa tgccaaggat catgttgttg cagatcttcg ccgcctggcc
      961 cgcgccggcg ccgccgcaat gcacgatctt cttgcccatg gcggccagca ccggctgcgc
     1021 gcgctcgaac gcgcccttct cgccgccgca catgaaggtg agcgtcgccg ccttcgcccc
     1081 gccggtgccg ccggagaccg gcgcatcgac cgactgcatc cctgcctccg ccgccagctt
     1141 gtgcacggcg cgggcgctgt cgacgtcgat ggtcgaggaa tcgatgaaca gcgcgctctt
     1201 cttcgccgcc gggatcagat cggcccagac cgcgcgtaca tgcttgccgg cgggcagcat
     1261 ggtgacgacg atgtcggccg ccttcaccgc cgcccttgca tccgccgcga cggcgacgcc
     1321 ggcctccgcc gccgcctttt tcgacgcctc cgacagatcg aagccggtca ccgcgtggcc
     1381 agccttcacg agattcgcgg ccattgggcc gcccatgttg ccgaggccaa tgaaagcgat
     1441 ggtggtcatg gaatgctcct acccctgatc aattcgctga atgcgatatg gcacagccct
     1501 accccatcat cttgcgcgcg acgatgaggc gcatgatctc gttcgtgcct tcgagaatgc
     1561 gatgcacgcg caggtctcgg gaaagtttct cgatgccgta ctcgtgcagg tagccgtagc
     1621 cgccgtggat ctgcagcgcc tcgtccacca cctgcgagcc ggtgtcggag gcaaagcgct
     1681 tggccatggc gcagcgcatg gtggtttctg ggtcctgccg gtcataggcg gcggcggcgg
     1741 catagagcat gacgcgcgcg gcctccagcc cggtcgccat gtcggcgagc ttgaactgga
     1801 gcgcctggaa ttcgttcagc gccttgccga aggccttgcg ctctgaagcg tagcgcacgg
     1861 acttctccag cgccgcctgc gcgccgccga gcgagcaggc gccgatgttg agccgtccgc
     1921 cgtcgagccc ggccatggcg atcttgaaac ctaggccttc ctcgccgaca cggttggcga
     1981 cgggtacgcg gcaattttca aaaatgacct ggcgggtcgg ctgggcgttc cagcccatct
     2041 tcttctcgtt cgcgccgaag gaaagacccg gcgcgccgtt ctcgaccaca aggcaggaga
     2101 tgccacccgc gcccggcccg ccggtgcgga ccatcgtcac ataggcgtcc gaggaaccgg
     2161 cgccggagat gaactgcttc gtgccgttga gcacgtagac atcgccgtcg cgctcggcct
     2221 tggtcttgag ggcggcggca tccgaaccgg cgcccggctc ggtgaggcag taggaggcga
     2281 tcgactccat ggtgaccagc ttcggcagga accgctggcg cagggcctcg tcgccatagc
     2341 ggtcgatcat ccaggccgcc atgttgtgga tggagatgaa ggcggacacg gcggggcagc
     2401 cggtggccaa cgcctccatg atcagcgcgg cgtccagacg gctgagaccg gaaccgccga
     2461 catcctcccg gacatagatg ccgcccatgc ccagcgccgc ggcttcgcgc atgacgtcga
     2521 cgggaaaatg cttctcctcg tcccatttca gcgcgttggg cgcgatccgc tcgtccgcga
     2581 aggcgcgcgc catgtcgcgg atggcaatct ggtcctcggt gagcgcgtag gtcatggcgt
     2641 catccctcga gcagcgcgta ggcgagctgg gcggtggcga cgggcttgcc gtcgctctca
     2701 gtggagatgg tggcctgcgc gaaggccatg gtgcggccga gccgggtgat acggaccgcg
     2761 cacagtaccg ccgagctcga taccgggcgc aggaaatgcg tggtctggtc caccgtcgtc
     2821 atcggccgcg atcgcccgga ggcggccgcc acggcgaaaa ccatcgcggt gtcggccagc
     2881 gccatcagcg cctgaccgca gatgatgcca cccgagcgtg tcagccggtt cgagaacggc
     2941 atgcgcagca ccacctcgtc gcgcgccacg gacatgactt ccagcttcag gtcctggatc
     3001 cagggggcga agacttcgcc caggagcttc ttcgccgcct caagatcgaa agtctccgcc
     3061 ctgtccatga cccgatcctc aggccgtgcc gcgacggc
//
LOCUS       DAOMXN010000257         3050 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_18079_length_3050_cov_11.674457, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000257 DAOMXN010000000
VERSION     DAOMXN010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18079_length_3050_cov_11.674457"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="PK812_13085"
     CDS             complement(<1..503)
                     /locus_tag="PK812_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HRJ70530.1"
                     /translation="MKALVYLGPNSLTYRDEAEPVPEPGEVLVRVEAVGICGSDMHAY
                     HGHDSRRPPPLILGHEAAGRIVGGSRDGARVTINPLVIDPSCVYARDGRGHLSPTRQI
                     ISMPPRPGAFAEYLRIPDGNALDIPDSLPIEHAALAEPIAVAWHAVRIGAERLFRPLA
                     ASRTVVLG"
     gene            complement(523..1293)
                     /locus_tag="PK812_13090"
     CDS             complement(523..1293)
                     /locus_tag="PK812_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRJ70531.1"
                     /translation="MFRLASLFDLTGRRALVTGGNSGIGLAMARALGLGGARVVLVAR
                     RQPELDQAAASLVAEGISAQVLAADLADPQAATHLAAACDRDALAIDVLVNAAGVNLR
                     QPFMGVTADAFDLHMALHLRAPFLLTQAFAPAMAQRGYGRIITIASLQSWRAFPNSAP
                     YGAAKGGVLQLTRAIAQEWSARGITCNAIAPGFFPTPLTEPVFSDPERAARNAAQTAI
                     GRNGAMSDLDGVTVFLASAASAYITGQTIAVDGGFTAK"
     gene            complement(1299..2606)
                     /gene="hisD"
                     /locus_tag="PK812_13095"
     CDS             complement(1299..2606)
                     /gene="hisD"
                     /locus_tag="PK812_13095"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010686704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HRJ70532.1"
                     /translation="MIYLKKASKSPETETETARKVVTEMLAEIDRRGEDAVKDYALKL
                     DRWSGPILVTAEEIERRTRDIPSGVKEDIRFAAAQVRLFAEAQRASITDFSIAPITGM
                     MAGQRMVPVNVAGCYVPTGRYAHIASAYMSVATAKAAGVKTVIACSTPFRGEGIHPEV
                     LYAMHVAGADIILTLGGVQAIATLANGLFTGKPADVVVGPGNKFVAEAKRMLFGKVGI
                     DVFAGPSEVAVIADASADPAIVASDLVGQMEHGHESPAWLFTTSRALAEDVMARIPGL
                     IAALPPTARDAAGAAWRDYGEVILCDTREEMATVSDRYASEHLEVQAADLDWWLANLT
                     NYGSLFLGEETTVAFGDKASGPNHILPTKFAARYSAGLSVHKFLKPLTWQRMDREACK
                     TIAQVSARISRLEGMEAHARTSDDRMAKYFPGHNFDLGRPVES"
     gene            complement(2614..>3050)
                     /locus_tag="PK812_13100"
     CDS             complement(2614..>3050)
                     /locus_tag="PK812_13100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HRJ70533.1"
                     /translation="GYPLVDVVEPAEHRRRFAEEVGARPVAPDALPRRYPLIVEASGK
                     AAARQLALESVAPLGAVVQIGEAERWDVEETRTIRRKDFFYIRSFYFPKSEYEENLAL
                     FRADRAIYARFVDASAGLQGLPELFNDFSSGRRIKPAVILPH"
BASE COUNT          510 a          995 c         1067 g          478 t
ORIGIN      
        1 cccagcacga ccgtgcgcga ggccgccaga ggccggaaca gccgttcggc gccgatgcgc
       61 acggcatgcc atgccacggc gatcggttcg gcgagcgccg catgctcgat cggcaggctg
      121 tccgggatgt cgagcgcgtt gccgtccgga atgcgcaggt attccgcgaa ggcgcccggc
      181 ctgggcggca tggaaatgat ctggcgcgtc ggcgagagat gcccacgccc gtcccgcgca
      241 tagacacagg atggatcgat gaccagcggg ttgatggtga cgcgcgcgcc gtcacgggaa
      301 ccgccgacga tgcgccctgc cgcttcgtgg ccgaggatca gcggtggcgg gcggcgcgaa
      361 tcatgaccgt gataggcatg catgtccgag ccgcagatgc cgaccgcctc cacgcgcacc
      421 agcacttcac cgggttcggg aaccggttcg gcctcgtcgc ggtaggtcag gctgttcggt
      481 ccgagataga ccagcgcttt caagggcgtg cctcctgtcg gatcacttgg cggtaaatcc
      541 gccatccacc gcgatggtct ggccggtgat gtaggcggaa gcggcggagg caaggaatac
      601 cgtgacgccg tccaggtcgc tcatcgcgcc gttgcggccg atggcggtct gggctgcgtt
      661 gcgcgcggcc cgttcgggat cggaaaacac cggttccgtc agcggcgtcg ggaagaaccc
      721 cggcgcgatc gcgttacagg taatgcccct ggcggaccat tcctgcgcga tagcccgcgt
      781 gagctggagc acgccgccct tggccgcgcc gtagggcgcc gagttgggaa aggcgcgcca
      841 ggactgcagg ctggcgatgg tgatgatgcg tccgtagccg cgttgcgcca tcgcaggagc
      901 gaaggcctgc gtcagcagga acggcgcgcg caggtgcagc gccatgtgca ggtcgaaggc
      961 gtccgctgtc acgcccatga acggctgacg caggttgacg ccggcggcat tgacgagaac
     1021 gtcgatggcc aaggcatccc tgtcgcacgc tgcggcgagg tgcgtcgcgg cttgcgggtc
     1081 agcaaggtcc gctgccagga cttgcgccga aataccctcg gccacgagcg atgccgccgc
     1141 ctgatccagt tccggctggc gccgggcgac gagcaccacg cgcgcccccc caaggccgag
     1201 cgcgcgtgcc atcgccaggc cgatgccgga attcccgccg gtgaccagcg cgcggcgccc
     1261 ggtcaggtcg aacagggagg caaggcggaa catcgcgatc agctctccac cggccgcccg
     1321 agatcgaagt tgtggccggg aaagtacttc gccatgcggt cgtccgacgt gcgcgcatgc
     1381 gcttccatgc cctcaagccg cgaaatgcgg gcagacacct gcgcaatggt cttgcacgcc
     1441 tcgcgatcca tgcgctgcca ggtcagcggt ttcaaaaact tgtgcaccga aagaccggcc
     1501 gaatagcgcg ccgcgaactt cgttggcagg atgtgattgg ggccggatgc cttatcgccg
     1561 aaggccacgg tcgtttcctc gcccagaaac agcgacccgt aattggtgag gttggccagc
     1621 caccagtcga gatcggcggc ctgcacttcg aggtgctcac tggcgtagcg gtcggagacg
     1681 gtcgccatct cctcccgggt atcgcacagg atgacttcgc cgtagtcacg ccacgccgcg
     1741 ccggcagcgt cgcgggcggt cggcggcagg gccgcgatca gcccgggaat ccgcgccatg
     1801 acatcctccg ccagggcacg cgaggtggtg aagagccagg ccgggctttc atggccatgt
     1861 tccatctgtc cgacgagatc cgaggcgacg atggccgggt ccgcgctggc gtcggcgatc
     1921 accgcgactt cggaggggcc cgcgaacacg tcgatgccga ccttgccgaa cagcatgcgc
     1981 ttggcttcgg ccacgaactt gttgccggga ccgacgacca catccgcggg cttgccggtg
     2041 aacaggccgt ttgccagcgt ggcgatggcc tggacaccgc cgagcgtcag gatgatgtcc
     2101 gcgccggcca catgcatggc atagagcact tcgggatgga tgccttcccc acggaacggc
     2161 gttgaacagg cgatgacggt cttgacgccc gccgccttgg cggtcgcaac cgacatgtag
     2221 gccgaggcga tatgggcata gcgcccggtg ggcacgtagc agccggcgac gttcaccggc
     2281 accatgcgct ggcccgccat catgccggtg atgggcgcga tggagaagtc ggtgatggag
     2341 gcgcgttgcg cctcggcgaa gaggcgcacc tgggcggcgg cgaagcggat gtcctccttg
     2401 acgccggacg gaatgtcgcg cgtgcgccgc tcgatctctt cggcggtcac gaggatgggg
     2461 cccgaccagc ggtcgagctt gagcgcgtag tccttcactg cgtcttcgcc gcgccggtcg
     2521 atctcggcca gcatttccgt caccaccttg cgggcggtct cggtctcggt ctcaggcgac
     2581 ttggacgcct ttttcaggta gatcatggat cactcaatgc ggcaggatga ccgccggctt
     2641 gatgcgccgg ccggacgaga agtcgttgaa cagttcgggc agcccctgga gtccggcgga
     2701 ggcatcgaca aagcgggcat agatggcgcg atccgcccgg aacaaggcga gattctcctc
     2761 gtactcactt ttcgggaagt agaacgagcg gatgtagaag aagtccttgc gccggatggt
     2821 ccgggtttct tccacatccc aacgctcggc ctcgccgatc tgcacgacgg caccgagcgg
     2881 ggcgacggat tcgagcgcga gctggcgcgc tgcggccttt cccgaagcct cgacgatcag
     2941 cgggtaacgc cggggcagag cgtccggcgc caccggccgc gcgccgacct cctcggcgaa
     3001 gcgtcggcga tgctccgccg gctcgacgac gtcgaccagc ggatacccca
//
LOCUS       DAOMXN010000258         2981 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_18710_length_2981_cov_10.867396, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000258 DAOMXN010000000
VERSION     DAOMXN010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18710_length_2981_cov_10.867396"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..971)
                     /locus_tag="PK812_13105"
     CDS             complement(3..971)
                     /locus_tag="PK812_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HRJ70534.1"
                     /translation="MSGFEILIAGPMMPMILDGLANRFRTHHLWKIDAPEAFLDREGA
                     RIQGLAGGFGSRVGATFLDRLPNLKIISNFGVGYDMVDAAEAGRRGIVVTNTPDVLSD
                     EVADSAIGLLISTVRKFPQAEKHLRDGHWLKGNFPLTASLRGRKMGIVGLGRIGKAIA
                     KRAAAFDIALCYHGRSQQADVDMPYYADLVEMARAVDILMVIVPGGPSTYRMINRQVL
                     EALGPDGVLINVARGSVVDEPALIEMLVERKILGAGLDVFENEPQVPRALLDLDNVTL
                     LPHVASASHHTRGLMAQLVMDNIYAIAEGRPPLTPVPETPWPHRPA"
     gene            complement(1065..1544)
                     /locus_tag="PK812_13110"
     CDS             complement(1065..1544)
                     /locus_tag="PK812_13110"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HRJ70535.1"
                     /translation="MTELKGKSIGHHLQLAARMHRARVAAAIQDLGLFPGQEQVLFAL
                     EAGDDMTVGQLARDLLVRPPTISKTLQRLALQGLVRRAEHENDARRSTVALTKEGRRR
                     TADLASRIDAVEEDLLAHFDGKEQRRLRKLLKRAAKALSSNDADADHEADEDDPHNA"
     gene            1618..>2981
                     /locus_tag="PK812_13115"
     CDS             1618..>2981
                     /locus_tag="PK812_13115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRJ70536.1"
                     /translation="MTAQDDSLVTLDVTGMTCAACSARVERALQAIDGVTSAAVSLPL
                     ERADIHVSGRVPVSHLVEAVEASGYHAVLRGQDTEGRRLARQERERQHGEDRRSTRLL
                     AGLAAAIATPFLIDMVVGVLSGTGHVLVPWLQALLATLCQLACGARFYRGAWFAMRAG
                     APNMDVLVAIGTSAAYGLSLFHWLADRAHHGGLYFEASVAILAFVLIGKVLEGEAKTG
                     TNAALRALAAATPQRAVRLNGEREEEVRADQLRPGDHVVLRPGSAAPVDGRIVSGAAA
                     FDESLITGESLPVSRAAGDAVVAGSVASGGKVVVEVVTTGDDTRLQRIARLIEDAEIS
                     KSPIQQLADRISAVFVPAVMAVAALSGVAWLLWGSSVEHAVLVTVSVLVVACPCALGL
                     ATPIALVAGANAAARAGLIVTEYAALEAAGRVSRVAFDKTGTLTLGAPSVVASAATEA
                     GRDTL"
BASE COUNT          480 a          947 c         1018 g          536 t
ORIGIN      
        1 ggtcaggccg gtcggtgtgg ccagggcgtt tcgggcaccg gcgtcagcgg cggccggccc
       61 tcagcgatgg cgtagatgtt gtccatgacg agttgcgcca tcaaccctcg ggtgtgatgc
      121 gaggcggacg caacgtgggg cagcagggtg acattgtcga gatcgagcag cgcccgagga
      181 acctgcggtt cgttttcgaa cacatccagt ccggcaccga ggatcttgcg ttctacgagc
      241 atttcgatca gcgctggctc gtccacgacg gaaccgcgcg ccacgttgat caggacgccg
      301 tcgggtccga gtgcttcaag gacctgccgg ttgatcatgc gataggtcga tggtccgccc
      361 ggcacgatca ccatcaggat atcgacggcg cgcgccatct ccacgaggtc ggcataatag
      421 ggcatgtcga cgtccgcctg ttggctacgt ccatgataac acagagcaat atcgaatgcg
      481 gcggcccgtt tggcgatggc cttgccaatg cgaccgagcc cgacgatgcc cattttccgg
      541 ccgcgcagcg aggctgtcag cgggaagttg cccttgagcc aatggccgtc tcgcaggtgc
      601 ttttcggcct gcgggaactt tcgaaccgtc gaaatcagca gtccgatggc cgaatccgcc
      661 acttcgtcgc tcagaacatc cggcgtgttg gtcacgacga tgccccgccg gcccgcttcc
      721 gccgcatcga ccatgtcgta gccgacgccg aagttcgaga tgatcttcag gtttggcaga
      781 cggtcgagaa aggtcgcgcc caccctggaa ccgaagccgc cggcaagccc ttgaattctg
      841 gcgccttctc gatccagaaa agcctccggc gcatcgatct tccacaagtg atgcgtgcga
      901 aaccgattgg caaggccatc gaggatcatc ggcatcatcg ggccggcgat caggatttca
      961 aaaccggaca aacgcttccc ccgtgcgtgc gacatgaatc gccgacaagc gtagcgccgc
     1021 cgccgcctgt atgacaactg gcaagctgcc cgggtgtcgc ggcttcaggc gttgtgcggg
     1081 tcgtcctcgt ccgcctcgtg atcagcatcg gcgtcgttgc tcgagagcgc cttggccgcg
     1141 cgcttgagga gcttgcgcag gcggcgctgt tccttgccgt cgaaatgggc tagcagatcc
     1201 tcttccacgg catcgatgcg ggaagcgaga tccgcggtgc ggcgacggcc ttccttggtg
     1261 agggccaccg tcgaccggcg cgcatcgttc tcgtgctcgg cgcgccggac caaaccttgc
     1321 agggccagcc gctgcagggt cttcgagatc gtcggcggcc gcaccagaag gtcgcgcgcg
     1381 agctggccaa ccgtcatgtc gtcgccggct tccaaagcga acagaacctg ctcctgtccc
     1441 ggaaacaggc cgaggtcctg gatcgcggcg gccacgcggg cacggtgcat gcgtgcggcc
     1501 aattgcaggt ggtggccgat ggatttgccc ttgagttccg tcattgcgcc cgcttattgc
     1561 tgccggaata aatcggtccg cttgaagaca agcacaaatc cgaccgtcgg agcaattatg
     1621 accgctcaag acgacagtct tgtgacgctg gatgtgacag gcatgacctg cgccgcctgt
     1681 tcggcgcggg tcgagcgcgc cctgcaggcc attgatggtg tgacgagcgc tgcggtcagc
     1741 ctgcccctgg agcgcgccga catccatgtg tcgggccggg tgccggtttc gcaccttgtc
     1801 gaggctgtcg aggcgagcgg ctatcatgcc gtgttgcgcg gtcaggacac cgagggccga
     1861 cgcctcgccc ggcaggagcg ggaacgtcag cacggggagg accggcgcag cacgcggctg
     1921 cttgccggcc tcgccgcggc gattgcgacg ccgttcctga tcgacatggt ggtcggggtc
     1981 ttgtccggaa cagggcatgt tctggtaccc tggctgcaag cgcttctcgc tacgctttgc
     2041 cagttggcct gcggggcgcg gttctatcgc ggcgcctggt tcgcgatgcg tgcgggcgcc
     2101 ccgaacatgg acgttctggt ggcgatcggc acgtcggccg cctacgggct cagcctgttc
     2161 cactggcttg ccgatcgtgc gcaccatggc gggctctatt tcgaagcatc cgtggccatc
     2221 ctagcctttg tgctcatcgg caaagtgctg gagggcgagg cgaaaaccgg gaccaatgcc
     2281 gcgctgaggg ctctcgccgc cgccaccccc cagcgggcgg tgcggctgaa cggcgagcgt
     2341 gaggaggaag tccgcgccga ccagctcagg ccgggcgatc acgtcgtgct gcgaccgggt
     2401 tcggcggctc ccgtggatgg acgcatcgtt tcaggagcgg cggccttcga tgaatccctg
     2461 atcaccggcg agagcctgcc ggtcagcagg gcggctggcg atgccgtggt cgccggcagc
     2521 gtcgccagcg gcggcaaggt tgttgtcgaa gtcgtcacca ccggggacga tacgcgtctg
     2581 caacgcatcg cccggctgat cgaggatgcg gaaatctcca agtcgccgat ccagcaactg
     2641 gcggaccgaa tctcggcggt attcgttccg gcggtgatgg ctgtcgcggc gctgagcggc
     2701 gtggcatggc tcctgtgggg atcgagcgtc gagcatgcgg tcctggttac ggtcagcgtg
     2761 ctggtggtgg cctgcccctg cgctctcggt ctggccacgc cgatagcact cgtcgcaggg
     2821 gcgaacgccg cggcgcgcgc cgggctgatc gttacggaat acgccgcact ggaggccgca
     2881 ggccgggtga gccgcgtggc gttcgacaag accgggaccc tgactctcgg tgctccgagc
     2941 gtggtcgcca gcgccgcgac cgaagccggc cgcgacacgc t
//
LOCUS       DAOMXN010000259         2868 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_19778_length_2868_cov_11.507288, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000259 DAOMXN010000000
VERSION     DAOMXN010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19778_length_2868_cov_11.507288"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2365)
                     /locus_tag="PK812_13120"
     CDS             complement(<1..2365)
                     /locus_tag="PK812_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HRJ70537.1"
                     /translation="MRLIGRFFAMLFATGTLVALVGVAAAAILVSYYSKDLPDHAQLE
                     KYEPPVMTRVHASDGSLLAEYAKERRLYLPIQAMPKLLKDAFLAAEDKNFYKHAGVDP
                     EGIARALLSNLKRSSGGRQQGASTITQQVAKNFFLSPEQSYERKIREMLVAFRLESTF
                     SKDKILELYLNQIYLGLGNYGVAAAALNYYGKSVHELTIPEAAYLAALPKAPSNYHPV
                     NQRDAAIIRRNYVIDRMVENGYIDREAGAKAKAEPLTIQLRQLSPNTYAAGFFAEEVR
                     RELAERYGSKKLYEGGLSVRSTLDTKLQVAARKTLADGLVRYDEARGWRGAVVKRPPG
                     GDWGLALAEAPVYSDVQPWQIAMVLDVTAAGARIGLQPRREANGSLSKARETGIIPPA
                     GVAWTRRTITTVLAPGDVIYVEPVANTQNQFRLRQVPEISGALVAMDPHTGRIVAMVG
                     GFSFEMSEFNRATQALRQPGSSFKPFVYATALDNGYTPSSIVMDEPIEVDQGPGVGVW
                     RPENYDGKFTGPRTLRYGITFSKNLMTVRLARDLGMPLVAEYAKRFGVYDDMLPVLAM
                     SLGAGETTVLRMTAAYSMFANGGRRIKPTMIDRIQDRWGQTIYKHDERQCALCKAEEW
                     RGQDEPKIVDKREQVIDPMSAYQITSIMEGVVQRGTATVIKEVNRTIAGKTGTTNDAK
                     DVWFVGYSPDLAVGVYLGFDKPKSLGDSATAGQYAAPIFRDFMKIALAGKPDLKFRIP
                     PQMKLIRVNASTGQRAGGSDGNTILEAFKPGTAPPESISYGSREGGAS"
     gene            complement(2494..>2868)
                     /locus_tag="PK812_13125"
     CDS             complement(2494..>2868)
                     /locus_tag="PK812_13125"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRJ70538.1"
                     /translation="ENKADLVAGVESKEDQEVVGDILFDLTRRETRQFSLSLARHLVG
                     SLQNRTAVHKNPLRSAGFRVLKAHDVPSVLVEVGYLSSSEDARLMKTSDWRAAMAESI
                     ADAVEKYIGENATGQANSGAKP"
BASE COUNT          490 a          936 c          905 g          537 t
ORIGIN      
        1 tggaggcccc gccctcgcgc gagccgtagc tgatgctctc gggcggcgcg gttcccggtt
       61 tgaacgcctc caggatggtg ttgccgtcgc ttccgcccgc gcgctgtcca gtgctggcgt
      121 tgacgcggat cagcttcatc tgcggcggaa tgcggaactt caggtcaggc ttgccggcca
      181 gggcgatctt catgaagtca cggaagatcg gcgcggcgta ttggcctgcc gtcgcggaat
      241 cccccagcga cttcggcttg tcgaagccga gataaacgcc gaccgcgaga tcgggagaat
      301 acccgacgaa ccacacgtcc ttcgcatcgt tggtggtgcc ggtcttgccg gcgatcgtgc
      361 gattgacttc cttgatcacc gtcgcggtgc cgcgctggac cacgccttcc ataatcgagg
      421 tgatctggta ggccgacatc gggtcgatca cctgctcgcg cttgtcgacg atcttcggct
      481 cgtcctgacc gcgccattcc tccgccttgc acagggcgca ctggcgctcg tcgtgcttgt
      541 agatggtctg gccccagcga tcctggattc ggtcgatcat cgtcggcttg atgcgccttc
      601 caccgttggc gaacatcgaa taggccgccg tcatgcgcag caccgtcgtc tcgccagcgc
      661 cgagcgacat ggcaagcacc ggcagcatgt cgtcatagac gccgaagcgc ttggcatatt
      721 ccgccaccag cggcatgccg agatcgcgcg ccagacgcac ggtcatcagg ttcttggaaa
      781 acgtgatgcc gtaacgcagc gtgcgcggtc cggtgaactt gccgtcgtag ttttccggcc
      841 gccagacgcc gacgcccggt ccctggtcga cctcgatcgg ctcgtccatc acgatgctgg
      901 acggggtgta gccgttgtcg agcgccgtcg cgtagacaaa cggcttgaac gaggagccgg
      961 gctggcgcag ggcctgcgtg gcgcgattga actcgctcat ctcgaaagag aagccaccga
     1021 ccatggcgac gatgcgcccg gtatgcgggt ccatcgccac gagcgcgccg gaaatctccg
     1081 gcacctgccg cagccggaat tgattctgcg tattggccac gggttcgacg tagatcacat
     1141 cgcccggggc aaggaccgta gtgatggtac gccgcgtcca ggcaacgccg gcgggcggga
     1201 taatgccggt ctcgcgtgcc tttgacagcg agccgttcgc ttcgcgccgg ggttgcaggc
     1261 cgatccgcgc gcctgccgcg gtcacgtcca gcaccatggc gatctgccag ggttgcacgt
     1321 cggaatagac cggcgcctcc gccagcgcga ggccccagtc gccgcccgga ggccgcttca
     1381 ccacagcgcc gcgccatccc cgcgcctcgt cgtagcggac aagaccgtcc gcaagggtct
     1441 tgcgcgcggc cacctgaagc ttggtgtcca gcgtcgagcg cacggacagg ccaccttcat
     1501 agagcttctt ggagccgtaa cgctcggcga gttcgcggcg gacctcctcg gcgaagaagc
     1561 cggcggcata ggtgttgggc gagagctggc gcagctggat cgtcagcggc tccgcctttg
     1621 ccttggcccc ggcctcgcgg tcgatgtagc cgttctcgac catgcgatcg atgacgtagt
     1681 tgcggcggat gatggccgcg tcgcgctggt tcaccgggtg atagttgctc ggcgccttgg
     1741 gcagcgccgc gagataggcc gcctcgggga tcgtcagttc gtgaaccgac ttgccgtagt
     1801 agttcagcgc cgccgccgcg acaccgtaat tgcccaggcc gagatagatc tggttcaggt
     1861 agagttcgag gatcttgtcc ttcgaaaagg tcgattccag ccggaatgcc accagcattt
     1921 cgcggatctt gcgctcgtag ctctgctccg gcgacaggaa gaagttcttg gccacctgct
     1981 gcgtgatcgt cgaagcaccc tgctgacggc cgcccgaaga gcgcttgagg ttggaaagca
     2041 gcgcacgcgc gataccttcg ggatcgacgc cggcgtgctt atagaagttc ttgtcttccg
     2101 ccgcgaggaa cgcgtccttg agcagcttcg gcatggcctg gatgggcagg tagagccggc
     2161 gttccttcgc gtactccgcc agcaggctgc cgtccgaggc atgcacgcgg gtcatcaccg
     2221 gcggctcgta cttctcgagc tgggcatgat ccggcaggtc cttggaataa tagctgacca
     2281 gtattgccgc agcggcaacg ccgacaagcg cgaccagcgt accggtcgcg aacagcattg
     2341 caaaaaaacg tccgatcagc cgcatccaaa cctacccgca acaggcttga gaaccgaaaa
     2401 gtacacgatt cccgcccact ctgccacgca cagagcggaa ggaaagcgcc agacaccctt
     2461 cgcgctttcc cttcaagtca caagcaggcg atttcatggc ttcgcgccgg aattggcctg
     2521 ccctgtggca ttttcgccaa tgtacttttc caccgcgtcg gcgatcgact ccgccattgc
     2581 tgcgcgccag tcgctcgtct tcatcagccg tgcatcctcg ctggacgaca gataccccac
     2641 ttccaccagc acggatggca cgtcatgggc cttgagcacg cgaaagccgg cggagcggag
     2701 cgggttcttg tgcacagcgg tacggttctg cagcgaaccg acgagatggc gcgccagcga
     2761 cagcgagaac tggcgcgtct cgcgccgggt caggtcgaac aggatgtcgc cgaccacttc
     2821 ctggtcttcc ttgctttcga cgccggcgac cagatccgcc ttgttttc
//
LOCUS       DAOMXN010000260         2858 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_19887_length_2858_cov_9.955048, whole genome shotgun sequence.
ACCESSION   DAOMXN010000260 DAOMXN010000000
VERSION     DAOMXN010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19887_length_2858_cov_9.955048"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="PK812_13130"
     CDS             complement(<1..252)
                     /locus_tag="PK812_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRJ70539.1"
                     /translation="MRRLKTAILGFALAAVFAPVALAYEIDPLTREPILPYQDTRVKA
                     TPIPREEISYSSKYAPGTIIVNTIERRLYYILGDGRAIRY"
     gene            542..616
                     /locus_tag="PK812_13135"
     tRNA            542..616
                     /locus_tag="PK812_13135"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:574..576,aa:Val,seq:cac)
     gene            complement(785..2449)
                     /locus_tag="PK812_13140"
     CDS             complement(785..2449)
                     /locus_tag="PK812_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRJ70540.1"
                     /translation="MRWTKGRIALLSLVVVGLAGWQLFSRYFYYLPGLVESFRDPLQS
                     HRPIAWNRGPDTAARTASERPPNIIVILADDLGWNDVTFNGGGVAGGSVPTPRIDSIA
                     RDGVSFMQGYAGNATCAPSRAAIMTGRYATRFGFEFTPTPKQFSRLVASYKLAGTTPG
                     HYHADREKDFPKNINDLVVPQSEVMIGQALKSRGYHTVMLGKWHLGGTETARPSARGF
                     DEALAFHEGAAMFLPENDPRVVNSKQEFDPIDKFLWANLRHAIRYNGGPSFQPDLYMT
                     DYLGREAARAIEANRNRPFMMYLAYNAPHTPLQALKSDYDALAHIADHRLRVYGAMIR
                     ALDRSVGVVLDALKAQGLEDNTLVIFSSDNGGANYVGLPDLNKPFRGWKATFFEGGLR
                     VPYFMKWPARIPAGVRYEKPIGHVDIFTTAVAAAEAAVPSDRTIDGVNLVPYVTGART
                     GEPHKALYWRSGHYKVVLSGNWKLQVSERPNKVWLFDLSTDPLEKNDLSRSHPAKLEE
                     LRKALAEIDGQQVPPSFPSLMEAPVLIDKPLNVEVRPDDELVFWAN"
BASE COUNT          461 a          939 c          943 g          515 t
ORIGIN      
        1 gtagcggatg gcgcggccgt cgccgaggat gtagtacagc cggcgctcga tggtgttcac
       61 gatgatcgtg ccgggcgcat atttcgagga ataggaaatc tcctcgcgcg ggatcggcgt
      121 cgccttcacg cgggtgtcct gatagggcag gatcggctcg cgtgtcagcg gatcgatttc
      181 ataagccagt gcgaccggcg cgaaaaccgc agccagcgcg aatcccagga ttgccgtctt
      241 caatcgacgc atcgccttgc aacctttcaa gaaatatcgg cggacgaatc cgcgcccccg
      301 cacgcggttg tggatcgctg cctcgatttt gacaagatcg ggaggccccg atcacgaggg
      361 ttgtgaacgc accgggccgc gagcgtggcc gtcctgcaac agtctctggt gggccggaat
      421 ggccaggatc gccgtgcgtc ggggcacgac gatcgcgcgc tccgtgccat ggcacgccag
      481 gccctccgtg atgggttctt gacttcgccg cactccatcc ctacgaagcc ccaaccttgt
      541 cgggcgcgta gctcagcgga agagcacttc gttcacaccg aaggggtcac aggttcgatc
      601 cctgtcgcgc ccaccattcc ttcaaagacg ctaatctctt gattcgcatt ggaaatgtgc
      661 aaatttcgca cttgtcctcg cgccgtcatg tcggctaccc gatcctggat tcggcaaccg
      721 gcaggcggga ctcgcgtatg cctgtgcggc aggagcacag ccacgatgcg cccggaccgg
      781 gacgtcagtt ggcccagaac accagctcgt cgtccggccg gacctcgacg ttcagcggtt
      841 tgtcgatgag gaccggggcc tccatgaggg acgggaagga cggcggcacc tgctggccgt
      901 cgatctcggc gagcgccttg cgcaattcct caagcttggc cggatggctg cggctgaggt
      961 cgttcttctc cagcgggtcg gtcgacaggt cgaacagcca gaccttgttg gggcgctccg
     1021 acacctggag cttccagttt ccggacaaga cgaccttgta atggccgctg cgccagtaca
     1081 gcgccttgtg cggctctccc gtccgcgccc ccgtgacata cggcacgagg ttgacaccgt
     1141 cgatcgtccg gtcgcttggc accgccgcct cggccgccgc cacggcggtg gtgaagatat
     1201 cgacgtggcc gatcggcttc tcgtagcgga cgccggcggg gatgcgcgcc ggccatttca
     1261 tgaagtaggg cacgcgcagc ccgccttcga agaacgtcgc cttccagccc cggaagggct
     1321 tgttgaggtc gggaagcccg acgtaattcg ccccgccgtt gtcgctggag aagatgacca
     1381 gcgtgttgtc ctccagcccc tgcgccttca gggcgtcgag cacgacgccg acgctccggt
     1441 cgagcgcgcg gatcatcgcg ccatagacgc gcagccggtg gtccgcgata tgcgccagcg
     1501 cgtcgtagtc ggatttcagg gcctgcaacg gcgtgtgcgg ggcgttgtag gcgagataca
     1561 tcatgaaggg gcggttgcgg ttcgcttcga tcgcccgcgc cgcctcgcgg ccgaggtagt
     1621 cggtcatgta caggtcgggc tggaacgagg ggccgccgtt gtagcggatc gcatgccgga
     1681 ggttcgccca caggaacttg tcgatcgggt cgaactcctg cttcgaattc acgacgcgcg
     1741 gatcgttctc cggcaggaac atcgccgcgc cttcatggaa ggccagggcc tcgtcgaacc
     1801 cccgggccga cggacgcgcc gtttccgtgc cgccgagatg ccacttgccg agcatcaccg
     1861 tgtgatagcc ccgggacttc agcgcctgtc cgatcatgac ctctgactga ggcacgacca
     1921 gatcgttgat attttttggg aaatccttct cccggtcagc gtggtagtgg cccggtgtcg
     1981 tccccgccag cttgtagctc gcgaccagtc tggagaactg cttcggcgtt ggcgtgaact
     2041 cgaagccgaa ccgcgtggcg taacggccgg tcatgatcgc ggcgcgcgag ggcgcgcagg
     2101 tggcgttgcc cgcatagccc tgcatgaagc tgacgccgtc gcgagcgatc gagtcgatgc
     2161 gcggggtcgg cacgctgcct ccggcgacac ccccgccatt gaaggtcacg tcattccagc
     2221 cgagatcatc ggcgaggatg acgatgatgt tcggcgggcg ctcggaggcc gtccttgccg
     2281 ccgtgtcggg tccgcgattc cacgcgatgg ggcgatgcga ctgcagcggg tcgcggaagc
     2341 tctccaccag accgggcagg taatagaagt atcggctgaa cagctgccag cccgcgaggc
     2401 caaccacgac caggctcagc agggcgatcc tgcctttcgt ccagcgcatg cgctccccct
     2461 ccgcgtcgcg gcgcccgtcg ccgaatggtc gcaggcgccg acgcatccta tcccaaccga
     2521 ttgccgtgtc gacacatgcc gtatggcttg acggcccttg gccagcgcgt ccagtctgcg
     2581 ccgatggagt acatgggtcc agcaccggca ggaaagtcga ccggcagggc gcgacagggc
     2641 gcggtcgcca gcctgcgctc cgcccttttc ctgcatctgc ggattgcggg caggagcccg
     2701 tgaaggtcct gcgcctttcc gccgtcgcgc ttctcgcgct ggcagtggct ggcatccatg
     2761 ggctcgccgt ccttcccctc tggacgcatc tcaccgagac tcgcgccgag atcgacgccc
     2821 ggcgtgagac ggcggcgcgc ctcaaggcgg ccgcggcg
//
LOCUS       DAOMXN010000261         2689 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_21655_length_2689_cov_11.856112, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000261 DAOMXN010000000
VERSION     DAOMXN010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21655_length_2689_cov_11.856112"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..165
                     /locus_tag="PK812_13145"
     CDS             <1..165
                     /locus_tag="PK812_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HRJ70541.1"
                     /translation="EGARLLAAGGRVLCVCAQAPTAEEARRLAYAGVGAIDWPEGFCR
                     RDIALRALGR"
     gene            261..1298
                     /locus_tag="PK812_13150"
     CDS             261..1298
                     /locus_tag="PK812_13150"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HRJ70542.1"
                     /translation="MEFLTTRLENFLNVAWTVFISIGSTFSLTSLACAFLVSAGYVAW
                     RRHSRGRAVKLRTVLAGMFPKRIWRGASMTADLWLFFLNAFVMGAIVGWAVLSFHFLG
                     GWITDGLNAAFGPRAPTAMPEWMARALFTLVLFLAYEFGYWLEHWISHRVPFLWEFHK
                     VHHSAEHLTPLTVWRMHPVNTVLFANVVGVVLAIAGSVAAWLLGGPLTEYAYSGTNVI
                     LIVFVHLYLHLQHSHIWISFTGWAGRIFMSPAHHQIHHSTNPLHFDRNMGSCLSLFDW
                     LFGTLHVPARQPEKLSFGIEAEGDVNTITATLVTPFVDSGRQLAKLATGGSANQEPVP
                     HPRSDAPLSST"
     gene            1365..2279
                     /locus_tag="PK812_13155"
     CDS             1365..2279
                     /locus_tag="PK812_13155"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013912719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HRJ70543.1"
                     /translation="MSVDAVAAARRGARALRFPAILPGRRVVRDLDGLPQVLGRIGSL
                     EVRLAVTAQDIRRAQRLRYAVFYEEMSATPSAKARLSRRDSDPFDRICDHLLVIDHAF
                     RRTPGARPRAKVVGTYRLLRQACAERNGGFYSAAEFDLRGLIAAHPGQHILELGRSCV
                     LKPYRNKRTVELLWHGIWRYVRHHDVDLMVGCASLEGTDPAELAVHLSFLHHHARAPA
                     EWQVSALPSRYVGMNRLRPEAFDAKKALASLPPLIKGYLRLGARYGEGAVVDRQFGTT
                     DVFVVLRVADINPRYIEFYGENAERHAA"
     gene            complement(2283..>2689)
                     /locus_tag="PK812_13160"
     CDS             complement(2283..>2689)
                     /locus_tag="PK812_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018041673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRJ70544.1"
                     /translation="LLRLAGIYGPGSSQVDSLRAGKARRIIKPGQVFNRIHVDDIAAT
                     CAALLAYRGCRRIWNVADNEPTPPQDVVAYAAGLLGMPPPPEEPFERAALSAMQSTFY
                     AENKRVSNQALREELGLPLRYPTYREGLASLV"
BASE COUNT          420 a          918 c          847 g          504 t
ORIGIN      
        1 gagggcgcgc gcttgctggc cgccggaggg cgcgtgctgt gcgtctgcgc gcaggcgccg
       61 accgcagagg aggcgcgtcg actcgcctat gccggagtcg gagctatcga ctggcctgaa
      121 ggattctgcc gccgcgacat cgccttgagg gcgcttgggc gctgacggtc gatttgccgc
      181 agagcaccaa ggccctgggt tagcggaacg tttacgtcag gcacctagcg tttcccgcga
      241 acactcgcgg agccgcgacc atggaatttc tcacgacgcg actggagaac tttctcaatg
      301 tcgcctggac tgtcttcatt tccatcggct cgacgttctc cctgacctcg ctggcttgcg
      361 ccttcctggt gtccgccggg tatgtggcgt ggcgccggca cagccgcggg cgcgccgtga
      421 agctgcgcac ggtcctcgcc ggcatgttcc cgaagaggat ctggcgcggc gcgtcgatga
      481 ccgccgacct ctggctgttc ttcctcaatg ccttcgtcat gggcgccatt gtcggctggg
      541 cggtgctgtc cttccatttc ctcggcggct ggatcaccga tggcctcaac gccgccttcg
      601 gaccgcgcgc ccccacggcc atgccggaat ggatggcacg ggccctgttc acgctcgtct
      661 tgttcctagc ctacgagttt ggctactggc tggaacactg gatcagccac cgcgtgccat
      721 tcctttggga gttccacaag gtccatcact ccgccgagca cctgacgccg ctcacagtct
      781 ggcgcatgca tcccgtcaat acggtgcttt tcgccaacgt ggtgggcgtc gtgctggcca
      841 tcgcgggaag cgtcgcggcc tggctactcg gcggtccgct gaccgaatac gcctattccg
      901 gaaccaatgt gatcctcatc gtgttcgtgc acctctacct gcacctccag cattcccaca
      961 tctggatatc tttcaccgga tgggcgggtc gcatcttcat gagcccggcc catcaccaga
     1021 tccaccactc gacgaacccg ctgcatttcg atcgcaacat gggcagttgc ctgtcactgt
     1081 tcgactggct gttcggcacg ctgcacgtgc ccgcaaggca accggagaag ctgagtttcg
     1141 gcatcgaggc cgagggcgat gtcaacacca tcaccgcgac gctggtgacg cccttcgtcg
     1201 attcgggccg gcaactggcg aagctcgcca ccggcggttc cgcgaaccag gagcccgtgc
     1261 ctcacccgcg ttccgacgcg ccgttgtcat cgacctgacg caggaatacc gtattcgcat
     1321 ccagaatgcc tgtaggaaac ggtcctggga cgcggacggg aaccatgtct gtagatgctg
     1381 tcgctgcggc gagacggggt gcgcgcgccc tgcgctttcc tgccattctc cctgggcgcc
     1441 gggtggtccg ggaccttgac ggactcccgc aagttcttgg gcgaatcggc tcgctcgaag
     1501 tgcggctggc cgtaacggcg caagacatcc gccgcgccca gcgcctgcgc tatgccgtgt
     1561 tctacgaaga gatgtccgcc accccgagcg cgaaagcccg gctctcgcgg cgcgacagcg
     1621 atccgtttga ccggatctgc gaccacctgt tggtgatcga ccatgcgttt cgccgcaccc
     1681 caggcgcgcg tccgcgcgcg aaagtggtcg gcacctaccg cctgttgcgg caggcatgcg
     1741 ccgagcgcaa tggcggcttc tattcggcgg cggagttcga cctgcgcggc ctgatcgccg
     1801 cgcatcccgg ccagcatatc ctggaacttg gccgatcctg cgtgctcaag ccctatcgca
     1861 acaagcgcac ggtcgagttg ctctggcatg gcatctggcg ctacgtgcgc catcatgacg
     1921 tcgacctgat ggtcggctgc gccagcctcg agggcacgga tccggccgaa ctcgcggtgc
     1981 atctcagttt cctccaccat catgcccgcg ccccggcgga atggcaggtt tccgcgctgc
     2041 cgtcccgcta tgtcggcatg aaccgcctgc gtcccgaagc cttcgacgcc aagaaggcgc
     2101 ttgccagcct gccgccgctc atcaagggct atctgcggct cggcgcgcgc tacggcgagg
     2161 gcgccgtcgt cgaccggcag ttcggcacga cggatgtgtt cgtggtgctg cgcgtcgccg
     2221 acatcaaccc gcgctacatc gagttctacg gcgaaaacgc cgaacgccac gcagcctgag
     2281 cgtcaaacaa gcgacgccag cccctcgcga taggtcggat agcgcagcgg caatcccagt
     2341 tcctcgcgca gggcctggtt cgagacgcgc ttgttctcgg catagaacgt gctctgcatc
     2401 gcagacaggg ctgcccgctc gaaaggctcc tcgggcggcg gcggcatgcc gagcagccct
     2461 gcggcgtagg cgacaacgtc ctgcggcggt gtgggttcat tgtcggcaac gttccagatt
     2521 cgccggcacc cgcgataggc aagcagggct gcgcaggtcg cggcgatgtc gtcgacatgg
     2581 atgcggttga acacctgacc cggcttgatg atccgccgcg ccttcccggc gcgcaggcta
     2641 tcgacctggc tgctgccggg cccatagatg ccggcgagac gcaacaggt
//
LOCUS       DAOMXN010000262         2639 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_22242_length_2639_cov_7.967879, whole genome shotgun sequence.
ACCESSION   DAOMXN010000262 DAOMXN010000000
VERSION     DAOMXN010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22242_length_2639_cov_7.967879"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="PK812_13165"
     CDS             complement(<1..711)
                     /locus_tag="PK812_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016920593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70545.1"
                     /translation="MREKAPDAFAQPQALWSRRGFWLVALAFALAWLAFGMPWLTGRV
                     TIPYDAKAHFQAQFQFLAKSLHEGQSPFWTPNVFGGAPQIADPQSLIFSPAFLVAWLV
                     PDPPFWVLDALVLAHLGVGGFAILMFFKDRGWHPAGAMVAALAFAFGAGAAWRIQHVG
                     QVFNFAEFGLTLWLLARALDRSSWRYGMLAGASGAMMVATPDQVALLGCYVLLGYVLW
                     RWLDGPGRAARFRRDLAPL"
     gene            1142..1993
                     /locus_tag="PK812_13170"
     CDS             1142..1993
                     /locus_tag="PK812_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006173690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRJ70546.1"
                     /translation="MKLVRYGRPGKEKPGIVDSQGRIRDLSKHVKDLSGEGLSPKSLA
                     KLAKLKVDRLPLVRGKPRLGPCVGNVGNFIAVGLNYADHAAETGAPIPKEPILFNKAT
                     SCIIGPYDEVVLPKGSKKTDWEVELVIIIGSRASYVSEKDALNHVAGYCVCNDVSERE
                     FQTERGGQWMKGKGCPTFGPIGPWLVTRDEIKDVQKLSMWLDVNDERMQTGSTATMIF
                     PVKHLVSYISQFMILEPGDIITTGTPPGVGLGMKPPRYLKAGDVVRLGIEGLGEQKQV
                     ISPHRAV"
     gene            complement(2063..>2639)
                     /gene="argC"
                     /locus_tag="PK812_13175"
     CDS             complement(2063..>2639)
                     /gene="argC"
                     /locus_tag="PK812_13175"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HRJ70547.1"
                     /translation="GCYPTGGIALLRPLVDAGLMSADHPVTVNAVSGYSGGGKSMIEA
                     YEKGAAPAFELYGLALEHKHLPELQLYSGLTRRPIFVPSVGNFRQGMLVSVPLHLDTL
                     PGKPRAADLQAALAARYKGSEWIAVAGEGDGALKSGKLEAEALNDSNRMELRVFGNEA
                     RRQAVLVARLDNLGKGASGAAVQNLRLMLGV"
BASE COUNT          488 a          871 c          881 g          399 t
ORIGIN      
        1 caggggcgcg aggtcacgac gaaaccgcgc cgcgcgtccg ggtccgtcca gccagcgcca
       61 caggacatag ccgagcagca cgtagcagcc gagcagcgcc acctgatccg gcgtcgccac
      121 catcatcgcg ccgctcgcgc cggccagcat gccataacgc caggatgacc ggtcgagagc
      181 acgcgccagc agccagagcg tcaggccgaa ttcggcgaag ttgaagacct gaccgacatg
      241 ctggatgcgc caggccgccc ccgccccgaa ggcgaaggca agcgccgcca ccatcgcgcc
      301 cgccggatgc cagccgcgat ctttgaaaaa catcaggatg gcaaagccgc cgacgccgag
      361 atgggcgagc accagggcat cgaggaccca gaatggcgga tcggggacca gccaggccac
      421 gaggaaggcc ggcgagaaga tgagggattg cggatcggcg atctgcggcg caccgccgaa
      481 aacgttgggc gtccagaacg gcgactggcc ctcatgaagg gacttggcga gaaactggaa
      541 ctgcgcctgg aaatgcgcct tggcgtcgta gggaatggtg acgcggcccg tcagccaggg
      601 catgccgaat gccagccagg ccagcgcgaa ggccagcgcg accagccaga agccgcgccg
      661 gctccacagc gcctggggct gcgcgaaggc gtcgggcgct ttctcacgca tgaggcctgg
      721 ttccaatcac acgcccgatg ccgggtttca cgaagttgtg atcttataca acacccttcc
      781 tgaaccgggc gcaagaattc cgttcatcgc ccgtgcatcc cggcttgcgg ctggccagtc
      841 ctgcgtgccg cgaatggcgg ctgcccccat gccgcaaggt tccccgcttt gcgccgaagc
      901 gtcagtccgg aacagtcgtg acctttactg ttgcggtgac acggcgagcc ctttctgcaa
      961 ccggagatgc gtactggcct gaccgttggc gataaccgtg ccgacttcgc ggccggcagt
     1021 ccccggccat cggcgggccg ccggcgcggc tatcgcggcg acgccaagac tcggcttggc
     1081 aagctccgag aattggtgtg aaaggaactg gggcttgcac tgtggcgtgc gggggtaggg
     1141 tatgaaactg gttcgttatg gccgtccggg caaggaaaaa cccggtattg tggattcgca
     1201 ggggcgcatt cgcgatcttt ccaagcatgt gaaagacctg tcgggcgagg gcctgtcgcc
     1261 gaagtctctg gccaagctcg ccaagctgaa ggtcgaccgt ttgccgctgg tgcgcggcaa
     1321 gccgaggctc ggcccctgtg tcggcaatgt cggcaatttc atcgcggtcg ggctgaacta
     1381 tgccgaccat gcggcggaaa cgggcgcgcc gatcccgaag gagccgatcc tcttcaacaa
     1441 ggcgacgagc tgcatcatcg gtccgtatga cgaagtcgtg ctgcccaagg gttcgaagaa
     1501 gaccgactgg gaagtcgagc ttgtcatcat catcggctcg cgggcttcct acgtctcgga
     1561 aaaggatgcg ctgaaccacg tcgcgggcta ctgcgtctgc aacgacgtgt cggagcgcga
     1621 gttccagacg gagcgcggcg gacagtggat gaagggcaag ggctgcccga ccttcggccc
     1681 catcgggccg tggctcgtta ccagggatga gatcaaggac gtgcagaagc tgtcgatgtg
     1741 gctcgacgtc aacgacgagc gcatgcagac cggcagcacg gcgacgatga tcttcccggt
     1801 caagcacctc gtcagctaca tctcgcagtt catgatcctg gagccgggcg acatcatcac
     1861 caccggcacg ccgccgggcg tgggcctggg catgaagccg ccgcgctacc tgaaggccgg
     1921 cgacgttgtc cgcctcggca tcgagggcct cggtgagcag aagcaggtga tttcgcctca
     1981 tcgcgcggtg tgaggcggcg ggcgcacgca gcgcccgaca tcccgggcga gcgatcgcga
     2041 cgcccgggtc gcgattcgcc cctcacacgc ccaacatcag cctgaggttc tgcacggccg
     2101 cgcccgatgc gcccttgccg agattgtcga gccgcgccac cagcaccgcc tgacgccgcg
     2161 cctcgttgcc gaagacgcgc agttccatgc ggttgctgtc gttcagcgcc tcggcttcca
     2221 gcttgccaga cttgagcgcg ccgtcgcctt cgcccgccac cgcgatccac tccgatccct
     2281 tgtagcgcgc ggcaagcgcc gcctgcaggt cggccgcccg gggcttgccc ggcagcgtgt
     2341 cgagatgcag cggcacgctc accagcatgc cctgccggaa gttgccgacg gagggcacga
     2401 agatcggccg ccgggtcagg ccggaataga gctgcagctc cggcaggtgc ttgtgttcca
     2461 gcgcgaggcc gtacagttcg aaggcaggcg cagccccctt ctcgtaggcc tcgatcatcg
     2521 acttgccgcc accggagtaa ccggagacag cattgaccgt gaccggatgg tcagccgaca
     2581 tcaggccggc gtcgaccagc ggccgcagca acgcgatgcc gcccgtggga tagcagccc
//
LOCUS       DAOMXN010000263         2580 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_22995_length_2580_cov_11.883960, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000263 DAOMXN010000000
VERSION     DAOMXN010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22995_length_2580_cov_11.883960"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..612)
                     /locus_tag="PK812_13180"
     CDS             complement(4..612)
                     /locus_tag="PK812_13180"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HRJ70548.1"
                     /translation="MSRFMQFIKARPITTVVVGAGLVLAGVTAVSAQYGPGWSGGPGW
                     GRGGPGWEGHGWHRGGGMMERGARFARFCSSEPQRYAPVARAWVKADLNLNAGQSAEF
                     DKLADVLLPALEQVKQEACNNFSARSAPAPEKLEKLAAVLRKAADAAEKSVAPAKTFY
                     AQLDEKQKARVEEMTERRGRGHHGWRGGFAAMGGALPIGWQR"
     gene            896..1195
                     /locus_tag="PK812_13185"
     CDS             896..1195
                     /locus_tag="PK812_13185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70549.1"
                     /translation="MRSYLTLLSSSSAVRDYAVAGIVLTVLSLVGFHSLSAFIESTQQ
                     SVRVQTAKIEKKDEATRQYTVTRSVLDDQVATGSIAGGKGRPIILDPCTGQIKSK"
     gene            1385..>2580
                     /locus_tag="PK812_13190"
     CDS             1385..>2580
                     /locus_tag="PK812_13190"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRJ70550.1"
                     /translation="MNFSDLGLSERVLAAVEASGYTTPTPIQAQAIPHVLQRRDVLGI
                     AQTGTGKTAAFTLPMLSILENGRARARMPRTLILEPTRELAAQVEESFNKYSGNQKLN
                     VALLIGGVSFGDQDAKITRGVDVLIATPGRLLDHFERGKLLLTGIDILVIDEADRMLD
                     MGFIPDIERICKLVPFTRQTLFFTATMPPEIQRVTEQFLSNPVRVEVARASSTASTIT
                     QKLVASGGKDFEKRDTLRRVIREQENFKNAIIFANRKRDVATLCKSLQRHGFNAAAIH
                     GDLDQPARMAALDSFRNGSIALLVASDVAARGLDIPDVSHVFNYDVPIHAEDYVHRIG
                     RTGRAGKLGTAISIVAKEDRKYLAAIEKLISRAVDWDGPNVDEFVPPPTEDRGEIRSL
                     RWRGRR"
BASE COUNT          453 a          882 c          796 g          449 t
ORIGIN      
        1 ttctcaccgc tgccaaccga ttggcaatgc gccacccatt gccgcgaatc cgcctcgcca
       61 gccgtggtgt ccccggccgc gccgctcggt catttcctcg acgcgcgcct tctgcttctc
      121 gtcgagctgg gcatagaagg tcttcgccgg agccacggac ttttctgccg cgtcggcggc
      181 cttgcgcaga acggcggcca gcttctccag cttctccggg gcaggggccg aacgggcgga
      241 gaagttattg caggcctcct gcttcacctg ctcaagcgca ggaagcagca catcggccag
      301 cttgtcgaat tccgccgact ggccggcatt gaggttcagg tcggccttga cccaggcgcg
      361 ggccacgggg gcatagcgct ggggttcgct cgaacagaag cgggcgaacc gggcgccgcg
      421 ttccatcatg ccgccgccgc gatgccagcc gtgcccctcc cagccgggac cgccgcgtcc
      481 ccatcccgga ccgccgctcc agcccggacc gtattgcgcg ctcaccgcag tcacgccggc
      541 gagcacgaga cctgcgccca ccactaccgt agtgatcggg cgcgccttga tgaactgcat
      601 gaatctggac atctgcaagc ctcatatctt gtcgcttgtc acccatggac aaagatagga
      661 gcgcaaccgg caggcgcaac ttaaggatat ttcatgttga gacagatcaa tttccggctg
      721 cacctccggc ttgttgtgcg tcgggcctcc ggatgctggc gacagcgtga cgtctctttc
      781 gcgaacgctc tctcaagccg ttgatttcat gaccttctcc tgggggatcg cgcaccgcgt
      841 aaacccggcc ttaactctga tcggtcagga tgactcgcaa tcaagggcag ggtccatgcg
      901 ctcctatctc acgcttctgt catccagttc cgccgttcgc gactatgccg tggcgggcat
      961 cgtgctgacg gtgctgtcgc tggtcggctt ccattcgctc agcgctttca tcgagagcac
     1021 gcagcaatcg gtgcgggtgc agaccgcgaa aatcgagaag aaggacgagg cgacgcgcca
     1081 gtacacggtg acgcgctcgg tcctagacga ccaggtcgcg accgggtcga tcgcgggcgg
     1141 caaggggcgt ccgatcatcc tggatccctg caccgggcag atcaaaagca agtgatccgg
     1201 cgtcccgcgt tgggctgcca cgcgcctgtc gcatagataa agtcattgat ttttcatgcc
     1261 gactccgtca tggaggcgtc gtgctcgcgg cataatgcgt atgctgcggg cgacatgcac
     1321 gtacggcggg ccatgccggt ccgcatttgg cgcgtccccg ggacgctccg gaaagaacgg
     1381 accgatgaat ttctctgacc tcgggctcag cgagcgcgtg ctcgcggccg tggaagcctc
     1441 cggctacacc acacccactc ccatccaggc gcaggccatc ccgcatgtgc tgcagcggcg
     1501 cgacgtgctc ggcatcgccc agaccggcac cggcaagacg gcggccttca cgctgccgat
     1561 gctgtcgatt ctcgaaaacg ggcgcgcccg ggcgcggatg ccgcgcacgc tgattctcga
     1621 accgacgcgc gaactcgcgg cgcaggtcga ggagagcttc aacaagtatt ccggcaacca
     1681 gaagctcaat gtcgcgctgc tgatcggcgg cgtgtcgttc ggcgaccagg atgccaagat
     1741 cacgcgcggc gtcgacgtgc tgatcgccac ccccggccgc ctgctggacc atttcgagcg
     1801 cggcaagctg ctgctgaccg gcatcgatat cctcgtcatc gacgaggctg accgcatgct
     1861 cgacatgggg ttcatcccgg acatcgagcg catctgcaag ctggtgccct tcacccgcca
     1921 gacgctgttc ttcacggcca ccatgccgcc ggagatccag cgcgtcaccg agcaattcct
     1981 gtcgaacccg gtgcgcgtcg aggtcgcccg cgcgtcgtcc accgcatcga cgatcacgca
     2041 gaagctggtc gcctccggcg gcaaggactt cgagaagcgc gacacgctgc gccgcgtcat
     2101 ccgagagcag gagaacttca agaacgccat catcttcgcc aatcgcaagc gcgacgtggc
     2161 gacgctgtgc aagtccctgc agcggcacgg cttcaacgct gcggcgatcc acggcgacct
     2221 cgatcagccc gcgcgcatgg cggcgctgga ttccttccgc aacggcagca tcgcgctgct
     2281 ggtcgcctcc gacgtggcgg cgcgcgggct cgacattccg gatgtcagcc acgtcttcaa
     2341 ctacgacgtg ccgatccatg ccgaggacta tgtgcatcgc atcggccgca ccggccgcgc
     2401 cggcaaactg ggaacggcaa tttccatcgt cgccaaggag gacaggaaat acctggccgc
     2461 catcgagaag ctgatcagcc gtgcggtcga ctgggacgga cccaatgtcg acgagtttgt
     2521 gcctccgccg accgaggatc gcggcgaaat ccgcagcctc cggtggcgcg gccggcggat
//
LOCUS       DAOMXN010000264         2510 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_23901_length_2510_cov_8.792668, whole genome shotgun sequence.
ACCESSION   DAOMXN010000264 DAOMXN010000000
VERSION     DAOMXN010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23901_length_2510_cov_8.792668"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1498)
                     /gene="metG"
                     /locus_tag="PK812_13195"
     CDS             complement(<1..1498)
                     /gene="metG"
                     /locus_tag="PK812_13195"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009449475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HRJ70551.1"
                     /translation="MAETFYLTTAISYPNGAPHVGHAYELMAADAIVRFQRLDGKDVY
                     FLTGTDEHGQKMLQTARKEGLPVRELADRNAARFRAMGQAFDATNNDFIRTSEPRHYE
                     ASKAIWKKMEAAGDIYLSKYSGWYSVRDEAYFDEDDITTDGEGRKFAPSGAPVEWVEE
                     ESYFFRLSAYQDRLLKYYEDNPDFIGPDARRNEVLSFVKRGLKDLSVSRNTFDWGVPV
                     PGGPGHVMYVWVDALTNYITGVGYPDEKSQLWKYWPADLHLIGKDIVRFHTVFWPAFL
                     MSAGIAPPKRVFCHGFVLNRGEKMSKSVGNVLDPFTLAEGYGPDAVRYFMLREVPFGQ
                     DGNYSHEAIVGRINADLANDFGNLAQRSLSMINKNCGGKVPACGVLTAADTALLAAVD
                     GALATCRAAMKDYALHQILNAIWALIGDTNRYFAAEEPWRLKKSDPARMETVLYVTAE
                     ALRQFAILTQPFTPRGATKLLDLLAIPADARSFAALGPAGRLVSGANLP"
     gene            complement(1606..>2510)
                     /locus_tag="PK812_13200"
     CDS             complement(1606..>2510)
                     /locus_tag="PK812_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70552.1"
                     /translation="KEAAARVDAALRGKTIGLVGRHPDGFDTCRYAADDLRRLAGIEV
                     APIALDEVFGRARKAEGDRIASHRRTVETLKGIGEVDQPQLDRSLRVYDSLDGLARDK
                     ALCGMAVRCWPEMFTEYGCAACGPMGMLNGRRIPAACEADVYGAVTALALQELSGASS
                     WLVDIVDLDAASDTGVFWHCGSAPLDMCDPDFAPEAQIHSNRRMPLLYQFPLKPGRIT
                     IARISQARNATKFVIGGGEVVRAPLSFTGTSAVVKFDGGASGMLRSLVGEALEHHVAI
                     AYGDHRAGLEALAARWGLPVLEIA"
BASE COUNT          431 a          833 c          805 g          441 t
ORIGIN      
        1 ccggcaggtt tgcgcccgag accagccggc cggccggtcc aagcgccgcg aagctgcgtg
       61 cgtcggccgg gatcgccagc aggtcgagca gtttcgtcgc gccgcgcggc gtgaaaggct
      121 gcgtcaggat cgcgaactgg cgcagcgcct cggcggtcac gtacaacacc gtctccatgc
      181 gcgccggatc gctctttttc agtctccacg gctcttcggc ggcgaaatag cggttggtat
      241 cgccgatcag cgcccagatg gcgttgagga tctggtgcag cgcgtaatcc ttcatcgcgg
      301 cgcgacaggt cgcgagcgcg ccgtcaaccg ccgccagcaa cgccgtgtcg gcggccgtta
      361 gcactccgca ggccggaacc ttgccgccgc agttcttgtt gatcatcgac agcgagcgct
      421 gcgccagatt gccgaagtca ttggctagat cggcgttgat gcgcccgacg atggcttcat
      481 ggctgtagtt gccgtcctgg ccgaacggca cttcgcgcaa catgaaataa cgcaccgcat
      541 ccggcccata gccctccgcc agcgtgaagg gatcgagcac gttgccgacc gactttgaca
      601 ttttctcgcc ccggttcagc acgaacccat ggcagaacac gcgtttcggc ggcgcgatcc
      661 cagccgacat caggaaggcc ggccagaaca cggtgtggaa ccgcacgatg tccttgccga
      721 tcaggtgcag gtccgccggc cagtatttcc ataactggct cttctcgtcg ggatagccga
      781 cacctgtgat gtagttggtc agcgcatcga cccagacata catgacgtgg cccggaccac
      841 ccggaaccgg aacgccccaa tcgaaggtgt tgcggctgac cgaaaggtcc ttgaggccgc
      901 gtttgacgaa actgagcacc tcgttgcggc gcgcgtcggg gccgatgaag tcaggattgt
      961 cttcatagta cttaagaagg cggtcctgat aggccgagag gcggaagaaa tagctctctt
     1021 cctccaccca ctccactggc gcgccggaag gcgcgaactt gcgtccctcg ccgtccgtgg
     1081 tgatgtcgtc ctcgtcgaaa taggcctcgt cgcgcaccga ataccagcct gagtatttcg
     1141 acaggtagat gtcgcccgcc gcctccatct tcttccagat cgccttggat gcctcgtagt
     1201 ggcgcggctc ggaggtgcga atgaagtcgt tgttggtggc gtcgaaggcc tgccccatcg
     1261 cgcggaaacg cgcggcattg cggtccgcga gctcgcgcac cggcaaccct tccttgcggg
     1321 cggtttgcag cattttctgg ccgtgctcgt ccgtaccggt caggaaatag acatccttcc
     1381 cgtcgaggcg ctggaagcgc acgatcgcat cggccgccat gagttcgtag gcgtggccga
     1441 catgcggcgc gccgttggga taggaaatgg cggtggtcag atagaacgtc tcggccatgc
     1501 gtcacctgta gcgggcgata tgcaagagtg cacgccttcc cgctgcgaat cccttatcgg
     1561 atcgagcgca tttttcctat gcgccgctcg gtgtgcgctc cgggatcagg cgatctccag
     1621 aaccggcagc ccccaacgcg cggcgagggc ttccagtccg gcgcggtgat cgccgtaggc
     1681 gatggcgacg tggtgctcca gcgcttcgcc gacgagggac ctgagcatgc ccgacgcgcc
     1741 gccgtcgaac ttcaccaccg ccgacgtgcc ggtgaaactc aacggcgccc gcaccacctc
     1801 cccgccgccg atcacgaatt tcgtcgcgtt cctggcttgc gagatgcggg caatggtgat
     1861 gcgtccgggc ttcaggggaa actggtagag cagcggcatg cggcggttcg agtggatctg
     1921 cgcctccggc gcgaagtccg gatcgcacat gtcgagcggc gccgaaccgc agtgccagaa
     1981 cacgccggta tcactcgccg catcgagatc gacgatgtcg acgagccagg acgaggcgcc
     2041 cgaaagctcc tgcagcgcca gcgccgtcac cgcgccgtag acgtcggcct cgcaggcggc
     2101 cggaatccgt cgtccgttca gcatgcccat cggcccgcag gccgcgcagc cgtattcggt
     2161 aaacatttcc ggccagcagc gaactgccat gccgcacagg gccttgtccc gcgccagccc
     2221 gtcgagcgag tcgtagaccc gcagcgagcg atcgagttga ggctgatcga cctcgccgat
     2281 gcccttcagc gtctcgaccg tccggcgatg gcttgcgatc ctgtcgcctt cggccttgcg
     2341 cgcgcggccg aacacctcgt ccagcgcgat cggcgcaacc tcgatgccgg cgaggcggcg
     2401 aagatcgtcg gccgcatagc ggcaggtgtc gaatccgtcg ggatggcggc caacaaggcc
     2461 gatggtcttg ccgcgcaggg ccgcgtccac gcgagccgcg gcttccttgg
//
LOCUS       DAOMXN010000265         2356 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_26191_length_2356_cov_9.218166, whole genome shotgun sequence.
ACCESSION   DAOMXN010000265 DAOMXN010000000
VERSION     DAOMXN010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26191_length_2356_cov_9.218166"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /locus_tag="PK812_13205"
     CDS             <1..828
                     /locus_tag="PK812_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC subfamily B transporter ATP-binding protein"
                     /protein_id="HRJ70553.1"
                     /translation="ASISELLASEPQVKEAAAPRPPVRPARGAIAFDGVDFAYPTRPE
                     ARTLKGFALEVASGERLAIVGPSGAGKSTILQLLLRFYDPQAGAIRIDGVPIRDLSLF
                     DLRSLIAHVPQEPVIFAATIAENIRLGRPDASDADVRRVAEMAAAHRFIERLPQGYDT
                     LIGERGVTLSGGERQRIAIARAMLRDAPILLLDEATSALDAENELAVQAALDAAMRGR
                     TSLVIAHRLATIRDADRIIVLDAGTVVESGSHAELVARNGLYAKLAALQFGIGEAAG"
     gene            825..1322
                     /locus_tag="PK812_13210"
     CDS             825..1322
                     /locus_tag="PK812_13210"
                     /inference="COORDINATES: protein motif:HMM:NF017470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HRJ70554.1"
                     /translation="MTGEPPRQPLPGERARDMLVLLGRWRIAALLFSDEGRIGRRALW
                     FYLLATMLLAALVFFSTTLRQSPPVHIAFAVLLLYPSYCVFSKRLQDIGVAGAFAIAV
                     VAISALDLVLAASGLKLRPGRLAALMQIWEWVGLANMAFVMVVLGLWPGNDGANRFGL
                     REPPW"
     gene            1480..1977
                     /gene="pal"
                     /locus_tag="PK812_13215"
     CDS             1480..1977
                     /gene="pal"
                     /locus_tag="PK812_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HRJ70555.1"
                     /translation="MFTAVVSPKLAKLAAAAVIAASLGACASKTEDPNLGAGGANVPG
                     SIQDFVVNVGDRVFFETDSTELTPQGVATLDKQAGWLNRYPRYSFTIEGHADERGTRE
                     YNFALGARRSETVRNYLAAKGVSAGRMKTISYGKERPVAVCNDISCWSQNRRAVTLLG
                     GAPGS"
     gene            2067..>2356
                     /locus_tag="PK812_13220"
     CDS             2067..>2356
                     /locus_tag="PK812_13220"
                     /inference="COORDINATES: protein motif:HMM:NF027655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgF trimerization domain-containing protein"
                     /protein_id="HRJ70556.1"
                     /translation="MHVPLPIAMKSLSLALVLLASVAAPSLAQTLDPAELLLRLDRLE
                     GENRRLNGQVEQLQFQIRRLEDQVRRFQSDVDFRFKESGGKGLPQAAAPPAPA"
BASE COUNT          347 a          803 c          806 g          400 t
ORIGIN      
        1 gcgagcatat ccgaactgct ggcgagcgag ccgcaggtca aggaggccgc cgcgccgcgc
       61 ccgccagtgc gccccgcgcg gggcgccatc gccttcgatg gcgtcgattt cgcctatccg
      121 acccggccgg aggcgcgcac cctgaagggg ttcgcgcttg aggtcgcgtc aggcgagcgg
      181 ctcgccatcg tcgggccgtc cggcgccggc aagagcacga tcctgcagct cctgctgcgc
      241 ttctacgatc cgcaggccgg ggcgatccgc atcgatggcg tgccgatccg cgacctgtcg
      301 ctgttcgatc tgcgcagcct gatcgcccat gtgccgcagg agccggtgat cttcgccgca
      361 acgatcgccg agaacattcg tctggggcgc cccgatgcca gcgatgcgga tgtccgccgc
      421 gtcgccgaga tggcggcggc ccaccggttc atcgagcgcc tgccgcaggg ctacgacaca
      481 ctgatcggcg agcgcggcgt gacgctgtca gggggcgaac gccagcgcat cgccatcgcc
      541 cgcgccatgc tgagggacgc gccgatcctg ctcctcgacg aagcgacctc ggcgctcgac
      601 gccgagaacg aactggcggt acaggcggcg ctcgatgccg ccatgcgggg gcgcaccagc
      661 ctggtgatcg cgcaccggct ggcgaccatc cgcgatgccg accgcatcat cgtgctcgac
      721 gcgggcacgg tggtggagag cgggtcgcat gccgaactgg tggcaaggaa cgggctctac
      781 gccaagctgg cggccttgca gttcggcatc ggcgaggcgg cgggatgacg ggggaaccgc
      841 cgcgccagcc gttgcccggc gagcgggcgc gcgacatgct ggtgctgctc ggccgctggc
      901 ggatcgccgc actgctgttc agcgacgagg ggcggatcgg ccgccgggcg ctctggttct
      961 acctgctggc gacgatgctg ctggcggcgc tggtgttctt ctcgaccacg ttgcgccaga
     1021 gcccgcccgt gcacatcgct ttcgccgtgc tgctgctcta tccgagctac tgcgtgtttt
     1081 ccaagcgttt gcaggacatc ggcgtcgccg gtgccttcgc gatcgcggtc gtcgccattt
     1141 ccgcgctgga cctcgtcctg gcggccagcg gtctgaagct ccggccgggg cggctcgcgg
     1201 cgctgatgca gatatgggag tgggtcggcc tggcgaacat ggccttcgtg atggtcgtcc
     1261 tcggcctgtg gccgggcaac gacggggcaa accgctttgg cctgcgcgaa ccgccctggt
     1321 gaggcgcgtg gctgcgccgc gcggcaagaa tttcttaacc aacggcgcga ttgcccggct
     1381 ttcgccttta tttggcaaga aggcgtaaag cttgacagtt cattgcatcg agagcgcgga
     1441 cccgcagttc cgctgacaga atcgactgga gaaatgccga tgtttaccgc cgtcgtgtcg
     1501 ccgaagctgg cgaaactcgc cgccgccgcc gtgatcgcgg cttccctggg cgcctgcgcc
     1561 tcgaagaccg aggacccgaa ccttggagcg ggcggcgcga atgtgcccgg ctcgatccag
     1621 gattttgtcg tcaacgtcgg tgaccgtgtg ttcttcgaga cggattcgac cgaactcacg
     1681 cctcagggcg tcgccacgct cgacaagcag gcgggatggc tcaaccgcta tccgcgctat
     1741 agtttcacca tcgaaggcca tgccgacgag cgcggcacgc gcgagtacaa cttcgcgctc
     1801 ggtgcccgcc gctcggagac ggtgcgcaac tatctcgccg ccaagggggt ttcggccggc
     1861 cgcatgaaga cgatctccta cggcaaggag cgtccggtcg cggtctgcaa cgacatttcc
     1921 tgctggtcgc agaaccgccg cgccgtgacg ctgctgggcg gcgcgccagg gtcgtgaggc
     1981 cggatggcac aggaacgacc ggaacgcgcc cggcgttccg gccccgttca tagtttggac
     2041 ggaatcatcg cgatcctgta ttcttcatgc acgtcccgct cccgatcgcc atgaaatctc
     2101 tgtcgctcgc tctcgtcctg ctggcttccg tcgcggcccc gtcgctggcc cagaccctcg
     2161 atcccgcgga gctcttgctg cgccttgacc ggctggaggg ggagaaccgc cgcctgaacg
     2221 gtcaggtcga gcaattgcaa ttccagatcc gccgcctgga ggatcaggtc cgccgcttcc
     2281 agtcggacgt ggacttccgt ttcaaggaat cgggcggcaa gggcctgccg caggcggccg
     2341 cccctccggc acccgc
//
LOCUS       DAOMXN010000266         2205 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_28806_length_2205_cov_12.373023, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000266 DAOMXN010000000
VERSION     DAOMXN010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28806_length_2205_cov_12.373023"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..500)
                     /locus_tag="PK812_13225"
     CDS             complement(90..500)
                     /locus_tag="PK812_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70557.1"
                     /translation="MSGSDARDMAARLLAHLETLTALVAEESHHLRKGRLREGLQLQG
                     RKDEAALAYGSALRGLGRQTIPPGPLRTALLDRHKAFEALLAENLAVLATVRSVSESI
                     LRDLSQKLADRPGGAYGPAARVKPPAPLALSRRT"
     gene            complement(487..792)
                     /locus_tag="PK812_13230"
     CDS             complement(487..792)
                     /locus_tag="PK812_13230"
                     /inference="COORDINATES: protein motif:HMM:NF021638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HRJ70558.1"
                     /translation="MKIPSRPPASPSDRVEPRDPKLWKTAQAFEAQFLKSMLEQAYAG
                     LSGEGPLGGSGPGADAWRGFLIDEHAKGMAAKDGIGIAASVYRHLQNLNRSSRDVRI"
     gene            complement(792..1910)
                     /locus_tag="PK812_13235"
     CDS             complement(792..1910)
                     /locus_tag="PK812_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HRJ70559.1"
                     /translation="MLLLRVCLLLVAVSAASGFFCGSAAAMSRIKDLAAIEGVRENQL
                     VGYGLVVGLNGTGDTLNNAPFTRQSLTAMLERLGVNTRGTTLRTANVAAVMVTANLPP
                     FSTQGTRMDVTVSALGDAKSLQGGTLLVTPLLGADGEVYAVAQGSLAISGFAAEGEAA
                     KLTRGVPTVGRIPNGALIEREVDFKLSGLKTLRFSLRNPDFTTARRIAAAVNDFLGAD
                     SAEPVDPGTVTVQVPKNYKGNIVQLLTEVEQLRVEPDQRARIIIDERSGVIVMGRDVR
                     VSTVGVAQGNLTVSISERPVVSQPEALSEGVTTVTPQTDIRVDTDPKKRLAIVREGVS
                     LRELVDGLNALGIGPRDIISILQAIKSAGALQADIEVM"
BASE COUNT          377 a          707 c          750 g          371 t
ORIGIN      
        1 aggagggcgc tggcttcaag ttcgcgcggc gacatggcgg cggtggcggt ttgcgcatac
       61 tggcgagcgg cgtgattcat ggatgttcct caggttctgc gggacagggc gagcggtgcg
      121 ggtggcttga cgcgggcggc cgggccgtag gcaccgccag ggcgatcggc gagtttctgc
      181 gacagatcgc gcaggatcga ttcggatacg cttcggacgg tggcgagcac ggccagattt
      241 tcggcaagca gcgcctcgaa ggccttgtga cgatcaagca gcgcggtcct gagcggtccg
      301 ggcggaatgg tctgccgacc aagcccgcgc aacgctgacc cataggcgag ggccgcctcg
      361 tccttgcgcc cctgcagctg caggccttcg cgcaggcggc ccttgcgcag gtgatgcgat
      421 tcctccgcca ccagcgcagt caatgtttcg aggtgggcga gcaggcgggc agccatgtcg
      481 cgcgcgtcag atccggacat cgcgactgct ccggttcagg ttctgcaagt gccgatagac
      541 cgaggcagcg atgccgatgc cgtctttcgc ggccatgccc ttggcatgct catcgatgag
      601 aaacccgcgc caggcgtcgg cgcccggtcc cgagccgccg agcggtcctt cgcccgacag
      661 gccggcatag gcctgttcca gcatgctctt gaggaattgc gcctcgaagg cctgcgccgt
      721 cttccacagc tttggatcgc gaggctcgac gcggtccgac gggcttgccg gcggtctcga
      781 cggtatcttc atcacatcac ctctatgtcg gcctgaaggg caccggcgga cttgatggcc
      841 tggaggatgg aaatgatgtc gcgcgggccg atgccgaggg cgttgaggcc atccacaagc
      901 tcgcgcaacg agacgccctc gcgcacgatg gcgaggcgct tcttgggatc ggtgtccacg
      961 cgaatgtcgg tctgcggcgt gaccgtggtg acgccctcgg acaaggcctc gggctgggag
     1021 accacagggc gttccgaaat ggaaacggtg aggttgccct gtgccacgcc gacggtggag
     1081 acgcgcacgt cccgacccat gacgatgacc cccgaccgct catcgatgat gatgcgggcg
     1141 cgctggtccg gctcgacgcg cagctgttcc acctcggtga ggagctgcac gatgttgccc
     1201 ttgtagttct tcggtacctg cacggtcacg gtcccaggat cgacaggctc ggccgaatcc
     1261 gcgcccagaa agtcgttcac cgctgcggcg atgcggcgcg ccgtggtgaa gtccggattg
     1321 cgcagcgaaa acctgagcgt cttcaggccg ctcagcttga agtcgacttc gcgctcgatc
     1381 agcgcgccgt tgggaatgcg cccgaccgtg ggcacgccgc gcgtgagctt ggcggcctcg
     1441 ccttcggccg cgaatcccga gatcgccaac gacccctggg ccacggcata gacctcgccg
     1501 tccgcaccga gcagcggcgt caccagcagg gttccaccct gtagcgactt cgcgtcgccc
     1561 agagccgaaa cggtcacgtc catgcgcgtg ccctgcgtgg agaagggcgg caggttcgcc
     1621 gtgaccatca ccgccgcgac attggcggtg cgcagcgtgg ttccgcgcgt gttcacgccg
     1681 aggcgttcca gcatcgcggt gagcgactga cgggtgaacg gggcgttgtt gagggtgtcg
     1741 ccggtcccat tgaggccgac gaccaggcca tagccgacca gctggttctc gcgcacgccc
     1801 tcgatggcgg cgaggtcctt gatgcgcgac atggccgccg ccgagccgca gaagaaaccg
     1861 gatgcggcag acactgcaac cagaagaaga cagacgcgca gaaggagcat ggggccaccg
     1921 cagaatgacg ccagattcct agcgagttcc gtgccgctgc aaatttgccg taacacattg
     1981 aaaaatagag atattatttt aatcgctgaa tgcggtggcc gaagctgccc gtcggcgtcc
     2041 ggcaagatcg accgggttgt taggaaagag ttaaccaatc ccggtcagtc ctgatgcaat
     2101 cagaaggtgc agttccatgc gcatcgccgc ccccggcacc accgtgcccc tgtcgcagcc
     2161 gcgcacgacg cgcgcggccg ccggcgcctt tcgtccggag cagcc
//
LOCUS       DAOMXN010000267         2199 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_28917_length_2199_cov_11.371269, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000267 DAOMXN010000000
VERSION     DAOMXN010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28917_length_2199_cov_11.371269"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1479)
                     /locus_tag="PK812_13240"
     CDS             complement(<1..1479)
                     /locus_tag="PK812_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HRJ70560.1"
                     /translation="MALPPPFRAAYAPADEVIASRLLAGATLDPAREAAVDQLATRLI
                     EAIRADQAGIGGVESVLREYSLTTKEGLALMVLAEALLRVPDSATADRLIEDKLGQGD
                     FAHHQPKSDAVLVNWGAWALGLTARIIQPGETPDGILAGLAKRIGLPAVRTATRQAMR
                     IMGSHFVLGETIEEALRRATAGDARRYRYSFDMLGEGARTARDAERYWHSYAAAIETI
                     GRNAGNDPLPNRPGISVKLSALHPKYEALNRDRVLAELVPKVIELARAAKTFDLNFTV
                     DAEEADRLELSLDVIAAVAADPSLAEWNGFGLAIQAYQKRAEQVIDYIDELARKLDRR
                     FMVRLVKGAYWDSEIKRAQERGLTDYPVFTRKPMTDLSYQACVRLLLARRPRLYPQFA
                     THNALTIATIVAMADGTEGYEFQRLHGMGEASYRALLADFPGAACRTYAPVGGHRDLL
                     AYLVRRLLENGANSSFVNVAGNPDMPVASLLRRPAALIGLARR"
     gene            1585..2055
                     /locus_tag="PK812_13245"
     CDS             1585..2055
                     /locus_tag="PK812_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003527496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HRJ70561.1"
                     /translation="MVTTHDLDRIDRRMLQLLQVDGRIATVDLAERIGLSPTATSERL
                     KRLYRDGYILGTTARLDPARIGRSFLVFIEVTLDKTTPDVFERFSAAVLRTPDVLECH
                     MVAGGFDYLIKTRVADMAAYRQLLGEVVLALPGVRETRTYAVMQEVKNDAPLPV"
BASE COUNT          383 a          735 c          683 g          398 t
ORIGIN      
        1 ccgtctggcg aggccgatga gcgcggccgg gcgcctgagc aggctcgcaa cgggcatgtc
       61 cggattgccc gccacgttca caaacgagga attcgcgccg ttttccagca gccggcgcac
      121 gagataggcc agaaggtcgc ggtgcccgcc gaccggcgca taggtgcggc aggccgcacc
      181 ggggaaatcc gccagcaggg cgcggtaact ggcctcgccc atcccgtgaa gcctttgaaa
      241 ttcatagcct tccgtcccat cggccatggc cacgatggtg gcgatggtca gtgcgttgtg
      301 ggtggcgaac tggggataga gccgggggcg ccgggccagc agcaggcgca cgcaggcctg
      361 ataggacaga tccgtcatgg gcttgcgcgt gaacacggga tagtcggtca gaccgcgttc
      421 ctgcgcgcgc ttgatctcgc tgtcccaata cgcgcccttg accagccgca ccatgaagcg
      481 gcgatccagc ttccttgcca attcatcaat gtagtcaatg acttgttcag cacgcttctg
      541 ataggcctgg atcgccagtc caaatccgtt ccactccgcc agtgaagggt ccgccgcgac
      601 ggcggcaatc acatccaggg agagttcgag ccggtctgcc tcctccgcat cgactgtgaa
      661 gttcaggtcg aatgtctttg ccgcacgggc cagttcgatg accttcggca cgagttcggc
      721 cagcacacgg tcgcgattga gcgcttcgta cttcggatgc agcgccgaga gcttcaccga
      781 gatgccgggc cggttcggca gcgggtcgtt gccggcattg cgcccgatcg tttcgatcgc
      841 ggcggcgtaa ctgtgccagt agcgttcggc gtcgcgcgcc gtgcgcgcac cttccccaag
      901 catatcaaag gaatagcggt accgacgggc atcgcccgca gtggcgcgcc tcagcgcctc
      961 ctcgatggtt tcgcccagga cgaagtggct gcccatgatg cgcatcgcct gccgtgtcgc
     1021 cgtgcgtacc gcaggcagcc cgatccgctt cgccagaccg gcgaggatgc cgtccggcgt
     1081 ctcgccgggc tggatgatgc gcgccgtcaa ccccagcgcc caggcgcccc agttcaccag
     1141 aaccgcatcg gatttcggct ggtgatgggc aaagtccccc tgccccagct tgtcttcgat
     1201 caggcggtcc gccgtggcgg aatccggcac gcgcaacagg gcctccgcca gcaccatcag
     1261 cgccagcccc tccttggtcg tcagcgagta ctcacgcaag accgattcca cgccgccgat
     1321 cccggcctga tcggcccgga tcgcctcgat caaccgggtc gccagctgat cgacggccgc
     1381 ctcgcgcgca gggtccagcg tcgcccctgc cagcaaccgg gaggcgatca cttcgtccgc
     1441 aggcgcatag gcggctcgaa acgggggcgg cagggccatg gcgattgttc tccgacaatg
     1501 cacctgtata tcgccaaatt ttccgtgtat cgattggcga aattcgcatc gtattagtgc
     1561 aattcaccat aaaaatggag ttatatggtg acgacacacg atctcgaccg catcgaccgg
     1621 cgcatgctgc aactgctgca ggtcgacggc cgcattgcaa cggtcgacct ggccgaacga
     1681 atcggcctct cgcccacggc cacgagcgaa aggctgaaac ggctctatcg cgacgggtac
     1741 attctcggca ccaccgcccg cctcgatccg gcgcgcatcg gccggtcgtt tctcgtgttc
     1801 atcgaagtca cgctggacaa gaccacgccg gacgtcttcg agcggttttc cgccgcggtc
     1861 ctgcgcaccc ccgacgtgct ggaatgccac atggtggccg gcgggtttga ctacctgatc
     1921 aagacccgcg tcgccgacat ggcggcctat cgccagttgc tcggcgaagt cgtcctcgcc
     1981 cttccgggtg tcagggaaac gcgcacctac gcggtcatgc aggaggtgaa gaacgacgcg
     2041 ccgctgccgg tctagaacga agaccgctac gcgatgccgt agtctttctt ggcctgttcc
     2101 agttccgctt cgtcgacgta gccgatgacg atcccgttct tcaattgcct ggcggcggaa
     2161 taggccgcga tggcctcgtc gctcatggtg atccggttg
//
LOCUS       DAOMXN010000268         2066 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_31631_length_2066_cov_9.668324, whole genome shotgun sequence.
ACCESSION   DAOMXN010000268 DAOMXN010000000
VERSION     DAOMXN010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31631_length_2066_cov_9.668324"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1362)
                     /gene="murJ"
                     /locus_tag="PK812_13250"
     CDS             complement(<1..1362)
                     /gene="murJ"
                     /locus_tag="PK812_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020091319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HRJ70562.1"
                     /translation="MLKNLLKVGGWTLVSRVTGFVRDILMAAILGAGPLMDAFAVAFR
                     LPNHFRAIFAEGAFANAFVPAYTATRTQKGDAAAHLFHGRILTLLLVSQLVLLAVALM
                     FTQPFVGLLAPGFKEKASVFPLAVELTRITFPYLMLITLVTIWADALASVKQFSAAAA
                     APVLLNLSMIATVAAAAWFKTPAHAAAWGVLLAGFLEAGLLFVAARRGGVLAAPKKPV
                     FDSDVRAFFKAFGPAVIGSAGVQIAMLADTVIVTFLATGGASALYYADRLYQLPIGVI
                     GVAAGTVLLPEMSRLIAASDPAGAHRAQNRAMGFTLLLTAPFVAAFVVIPDLIVAGLF
                     RRGAFDDAAALATASVLAAYTVGLPAVVLIRSAVASFTARGDTRTPLWASLTAIACNL
                     LLKLMLWRSMGAAGLALATAVGAWINFAILAVLALRGDKAAPDRLLLRTLMVATLAAL
                     ALAG"
     gene            complement(1425..>2066)
                     /locus_tag="PK812_13255"
     CDS             complement(1425..>2066)
                     /locus_tag="PK812_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014889860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRJ70563.1"
                     /translation="EGYDMVYGQRVDRSTDGPVRRWLTQRFYKLFARFGETALPQGAG
                     DYRLMDRKVVDALKRMGERARFSKGLYAWVGFKSVGIPFEVQDREHGSSKWSLRKLLG
                     FALDGLTSFSTLPLKVWTYVGLAVSGFALGMALFYLLKTLIYGVDVPGFPSLIVSVMF
                     FSGVQLMSLGILGEYIGRIFAEVKRRPLYIVAEEIGAEHPVATGARREDVAAR"
BASE COUNT          424 a          692 c          677 g          273 t
ORIGIN      
        1 gccggcgagc gccaacgcgg cgagtgtggc gaccatcagg gtgcgcagga gcagccggtc
       61 gggcgccgcc ttgtcaccgc ggagcgccag cacggccagg atagcaaagt tgatccaggc
      121 gccgacagcc gtggccagcg ccagtcctgc ggcgcccatc gagcgccaca gcatgagctt
      181 cagcaggaga ttgcaggcaa tggcggtcag cgaggcccag agcggtgtcc tcgtgtcgcc
      241 gcgtgcagtg aaactggcga cggcactgcg gatcagcact accgccggca agcccacggt
      301 gtaggccgcg aggacgctgg ccgtcgccag cgccgccgca tcatcgaagg cgccgcgcct
      361 gaaaagtccg gccacgatga gatcgggaat gaccacgaaa gccgcgacga aaggagcggt
      421 cagcagcagg gtgaacccca tcgcgcggtt ctgcgcccga tgagcgccgg ccggatcgct
      481 ggccgcaatc aaccggctca tctccggcaa cagcaccgtg ccggcagcca ccccgatcac
      541 gccgatcggc aactggtaga gacggtcggc gtagtacagc gcggaagcgc cgccggtcgc
      601 caggaaggtg acgatcaccg tatcggccag catggcgatc tgcacgccgg ccgatccgat
      661 cacggccggg ccgaaggctt tgaagaaggc gcggacatcg ctgtcgaaca ccggtttttt
      721 gggcgccgcc agcacgccac cgcgccgcgc ggcaacgaac agcaggccgg cttcgaggaa
      781 tccggcgagc agcacgcccc aggctgcggc atgcgccggc gtcttgaacc aggcggcggc
      841 ggcgaccgtt gcgatcatcg aaaggttcag cagcaccggc gcggccgcgg cggccgagaa
      901 ctgcttcacc gatgcgagcg cgtcggccca gatcgtcacc agcgtgatca gcatgagata
      961 ggggaaggtg atgcgcgtca gctccaccgc gaggggaaag acactggcct tttccttgaa
     1021 tcccggcgca agcagcccaa cgaagggctg cgtgaacatg agcgccaccg ccagcagcac
     1081 gagttgtgac accagcagca acgtcaggat gcgaccgtgg aacagatgcg ccgccgcatc
     1141 gcccttctgc gtgcgcgtcg cggtataggc cggtacgaag gcgttggcga atgcaccttc
     1201 cgcgaaaatg gcgcggaaat ggttgggcag ccggaaggcg acggcgaaag catccatcag
     1261 cggacctgcc ccgagaatgg ccgccatcag gatgtcacgc acaaagccgg tgacgcgcga
     1321 aaccagcgtc caccccccga ccttgagcag gttcttcagc atccccgtct catgcgcgat
     1381 tcagtccggc ggggcaattg gcgcgtgccg atgcggggca ggccttaccg cgccgccaca
     1441 tcctcgcgcc gcgcgccggt cgctaccggg tgctccgcgc caatctcctc cgcgacgata
     1501 tagagcggtc gccgcttcac ctcggcgaaa atgcggccga tgtactcgcc caggattccg
     1561 agcgacatca actggacgcc ggagaagaac atcaccgaga cgatcagcga gggaaagccc
     1621 ggcacgtcga caccgtagat cagcgtcttg agcaggtaga acagcgccat gcccagcgcg
     1681 aaaccggaga cggcgagacc aacgtaggtc cataccttga gaggcagggt cgagaacgag
     1741 gtcaacccat ccagtgcgaa gccgagaagc ttgcgcaggc tccacttgct ggagccgtgc
     1801 tcgcgatcct gaacctcgaa cggaatgccg acggacttga atccgaccca ggcgtaaagc
     1861 cccttcgaaa aacgtgcccg ctctcccatg cgcttgagcg catccaccac cttgcgatcc
     1921 atcagccgat agtcgccggc gccctggggc agggcggttt caccgaaacg cgcgaacagc
     1981 ttgtagaacc gctgggtcag ccagcgccgc accggcccgt cggtcgaacg gtcgacgcgc
     2041 tggccataga ccatgtcgta gccttc
//
LOCUS       DAOMXN010000269         2054 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_31899_length_2054_cov_7.624312, whole genome shotgun sequence.
ACCESSION   DAOMXN010000269 DAOMXN010000000
VERSION     DAOMXN010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31899_length_2054_cov_7.624312"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="PK812_13260"
     CDS             complement(<1..586)
                     /locus_tag="PK812_13260"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRJ70564.1"
                     /translation="MSADFEFTIHARDGKARTGAITMPRGVIRTPVFMPVGTAGTVKG
                     MYLDQVRALGADIILGNTYHLMLRPGAERVADLGGLHDFARWPWPILTDSGGFQVMSL
                     ASLRKLDEQGVTFKSHIDGSMHHLTPERSVEIQIKLDSNIVMQLDECIALPAEEADVA
                     RAMRLSLRWAERCKAAFGVQPGRALFGIVQGGTSE"
     gene            1052..>2054
                     /locus_tag="PK812_13265"
     CDS             1052..>2054
                     /locus_tag="PK812_13265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ70565.1"
                     /translation="MLRRPLLARLFTDAGYARVQEMSTWATPVSLTLIGLAADFSQLF
                     GEVVFTLLWLSAVATVVFGVAVLLRTRFCVTCAKPLVVAVLATLWFGGIFGGQQLLAA
                     SKQGVLAKLFPQASEIQLAIYARLEDIGVLAKKIDRTTEQTAADVKRLHDKQDSLEKK
                     FDALAAQIVRSKLNESTVPASPSAREGAEKAVGEAIADVAKGQDSASKQAFEALQRGD
                     TSVAERLFAKVLEEKTKEGRAANREAAEAARNLAAFKRLTDVAAARDLYERAASLDPD
                     HLWTWIDLGDMRLASGQTGAALEAYGKAEAAARRSGDERDLSVSHNRIGDVRRAQGDL
                     "
BASE COUNT          372 a          655 c          691 g          336 t
ORIGIN      
        1 cctcgctggt cccgccctgc acgatgccga acagcgcccg gcccggctgg acgccgaagg
       61 ccgccttgca gcgctccgcc cagcgcagcg acaggcgcat ggcgcgcgcc acgtccgcct
      121 cctccgccgg cagggcgatg cactcgtcga gctgcatgac gatgttggaa tccagcttga
      181 tctggatctc gaccgagcgt tccggggtca gatggtgcat cgagccgtcg atatgcgact
      241 tgaaggtgac gccctgctcg tccagcttgc ggagcgaggc cagcgacatg acctgaaagc
      301 cgccgctgtc ggtgaggatc ggccagggcc agcgcgcgaa gtcgtgcagg ccgccgaggt
      361 ccgccacccg ctccgcgccg gggcgcagca tgagatgata ggtgttgccc aggatgatgt
      421 cggcgccgag cgcgcggacc tgatcgagat acatgccctt gacggtgccg gccgtgccga
      481 ccggcatgaa caccggcgtg cggatgacgc cgcgcggcat ggtgatggcg ccggtgcggg
      541 ccttgccgtc gcgggcgtgg atggtgaact cgaagtctgc gctcatggcg gtctgtcagc
      601 acgaagcggc ggcgggatca agcgtcacgg cggggatcgt ggcggcccgc gccggcgcaa
      661 gacgaagcag gaccagtaaa cctcaagcaa aacggggcgg aactttccgg aggcgaccaa
      721 tacggcagat caatcttgcg cgctgttctt gccgcgccca tattcaccgc agcaggttgg
      781 ggcaagggat ggcgccttcg agggtacccc ttcatgtgat cgaggcaaac ggagcgttta
      841 gagcggtatg agcgacgcag gctccaagga cccgcccgga tcgggatcaa ggcagcaaga
      901 gccggctgcg agtgcgcact tttcctatgg tgggaagctt ggatttacgc cgaaagtgag
      961 tttcggcgcg cctaccgccg tgccacccaa ggctacgaag gcaccgacga cgaagcctgc
     1021 aacggctcca gaacccgctt cgcccgcacc cgtgctcagg cgccccctgc tcgcccgttt
     1081 gttcaccgat gccggttacg cccgggtgca ggaaatgagc acctgggcga cacccgtttc
     1141 attgacgttg atcggcctcg ccgccgattt cagccagttg tttggcgagg tggtgttcac
     1201 ccttctgtgg ctttcggcgg ttgcgactgt ggtgttcggc gtcgcggttc tgctgcgcac
     1261 acggttctgt gtcacctgcg ccaagccgtt ggtcgtcgcg gttcttgcga cgctctggtt
     1321 cggcggcatt ttcggcggcc agcaactgct cgccgcatcg aagcaaggcg tcctcgccaa
     1381 attgttcccg caagcctcgg aaatccagct ggccatttat gcaaggctgg aggacatcgg
     1441 cgttctcgcc aaaaagatcg acaggacgac ggaacaaacc gccgccgatg tcaaaaggct
     1501 gcacgacaag caggattcgc tggagaagaa attcgatgcg ctcgcagcgc aaatcgtcag
     1561 atcgaaactg aatgaatcga cggtaccggc ttcgccttcc gcgcgcgagg gtgcggaaaa
     1621 agctgtcggc gaggccatcg ccgacgttgc gaaggggcag gacagcgctt cgaagcaggc
     1681 cttcgaagcc ctgcaacgtg gtgacacttc cgtcgccgag cggttgttcg ccaaggtgct
     1741 ggaagagaag acgaaggagg gccgcgccgc caaccgggag gcagccgagg ccgcgcgcaa
     1801 cctcgccgca ttcaaacgcc tgaccgatgt cgccgccgcg cgcgacctct acgaacgcgc
     1861 cgcctcgctc gaccccgacc atctctggac atggatcgat ctcggcgaca tgcggcttgc
     1921 gtccgggcag accggcgcgg cgctggaggc ctatggcaaa gcggaagcgg cggcccggcg
     1981 cagcggggat gagcgcgatc tctcggtctc ccacaacagg atcggcgatg tccgccgcgc
     2041 gcagggcgat ctcg
//
LOCUS       DAOMXN010000270         1973 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Beijerinckiaceae bacterium isolate AMR_MDS_5651
            NODE_33868_length_1973_cov_10.915537, whole genome shotgun
            sequence.
ACCESSION   DAOMXN010000270 DAOMXN010000000
VERSION     DAOMXN010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867872
            Sequence Read Archive: SRR1506983
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Beijerinckiaceae bacterium (wastewater metagenome)
  ORGANISM  Beijerinckiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.52
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:21:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,655
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,603
            CDSs (with protein)               :: 2,603
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 14 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Beijerinckiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33868_length_1973_cov_10.915537"
                     /isolate="AMR_MDS_5651"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1978229"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..787)
                     /locus_tag="PK812_13270"
     CDS             complement(83..787)
                     /locus_tag="PK812_13270"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018261458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRJ70566.1"
                     /translation="MMIDPRKAAGTAEHDASRRTGPERFSRRSFLAGSAAGVSALALG
                     GCITDGLSQTEVDRMYAAVPDKKFPIPASDVSQVERKYYRRKVKYDSDEVAGTIVVDS
                     ANYFVYRIEGEGEATRYGANVGRQGFLWSGEAYVGRKAEWPTWTPPPEMIKRQPEVAK
                     YARGMPGGLENPLGARTLYLYQKGVYTLYTIYSTSDPETIGTNLTSGCTGLLTQDMID
                     LYDRTAVNTKVIVLPA"
     gene            complement(1087..1878)
                     /locus_tag="PK812_13275"
     CDS             complement(1087..1878)
                     /locus_tag="PK812_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006937996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRJ70567.1"
                     /translation="MLVIETVSKSFAPPRRLFAQGKPVQALSDVSLSVGEGECVGLVG
                     ESGSGKTTLTRCILRLDEPTSGRILFEGQDLARLTPAALRAVRARIQIVFQDPYASLN
                     PRMSIHDIVTEPMLIHRRVLGLDGRQRTDRAAELLALVGLGTHYLHRYPHEFSGGQRQ
                     RIGIARALATNPRLILLDEPTSALDVSVQAQVLNLLCDLQQRLSLTYLFISHDLSVIR
                     YICDRVAIIYRGEIVESGATAAVFEKPQSDYTHALLAAMPEIAIR"
BASE COUNT          333 a          632 c          657 g          351 t
ORIGIN      
        1 gctgcaccga ctgccgcggc aaccccggcg ctgccgccgc cggcgacgac cacgtcatag
       61 cgggcgggcg gtcgggtccg cgtcatgccg gcaggacgat caccttggtg ttgactgccg
      121 tgcggtcata aagatcgatc atgtcctgtg tgagcaggcc ggtgcagccg ctcgtcaggt
      181 tggtcccgat ggtctcggga tcggacgtgc tgtagatcgt gtagagcgtg tagacgccct
      241 tctgataaag atagagcgtg cgggcgccga gtggattttc cagtccgccc ggcatgccgc
      301 gcgcatattt cgccacttcc ggctggcgct tgatcatctc cggcggcggc gtccaggtcg
      361 gccattccgc cttgcgcccg acataggcct cgccgctcca caggaagccc tggcggccga
      421 cgttggcgcc atagcgggtg gcctcgccct cgccttcgat gcggtagacg aagtagttcg
      481 cggaatcgac cacgatcgtc ccggcgactt cgtcggagtc gtatttcacc ttgcggcgat
      541 agtatttccg ctccacctgg ctgacgtcgg aggcggggat cgggaacttc ttgtcgggta
      601 ccgccgcgta catccgatcc acctcggtct ggctcaggcc gtcggtgatg cagccgccca
      661 gggccagagc gctgacacca gcggcggaac cggcgagaaa cgagcggcgg ctgaagcgtt
      721 ccggtccggt ccggcgcgat gcgtcatgct ccgccgtgcc ggcggccttg cgggggtcga
      781 tcatcatgat ggtgaagttt cccatatccc gttacgcaac cagagaaacc tccggcacac
      841 acgcgctgac tgcactcagc cggccaagaa tgccatcata ccgagggtca tgacaatgga
      901 cccctggttc cctttgttga attttccttg agcattgtaa aacaaggaga attcaagagc
      961 gccatcagtc attcctgatg acggatgggt aacaggcggc cctgcctggc aatggccggg
     1021 gggccatggt cggcctgtcg atgccggatt catcgccggc cacatcccgc tcggccctgt
     1081 ccatcgtcag cggatggcga tttccggcat cgcggccagc aaggcgtggg tgtagtcgct
     1141 ttgcggcttc tcgaacaccg cggctgtcgc gccgctctcg acgatctcgc cccggtagat
     1201 gatcgccacg cggtcgcaga tatagcgaat gaccgaaaga tcgtggctga tgaacagata
     1261 ggtcaggctc aaccgctgct gcagatcgca caggaggttc agcacctgcg cctgcacgga
     1321 aacatcgagg gcggaggtcg gttcatccaa gaggatcagc cggggattgg tcgcgagcgc
     1381 acgcgcgatg ccgatgcgct ggcgctgccc gccggaaaac tcgtgcgggt atcgatgcag
     1441 gtaatgcgtt cccagcccga cgagcgccag cagttcagcg gcgcggtccg tccgctggcg
     1501 cccgtcaagg cccagcaccc gccggtggat cagcatcggc tcggtcacga tatcgtggat
     1561 cgacatgcgc gggttgagcg aggcgtaggg gtcctggaaa acgatctgga tgcgggcgcg
     1621 caccgcgcgc aaggccgccg gtgtcagcct cgcgaggtct tgcccctcga acaggatgcg
     1681 gccgctcgtc ggctcgtcca gcctgagaat gcagcgtgtc agcgtcgtct tgcccgagcc
     1741 ggattcgccg acaagtccga cgcactcgcc ttcgccgacg ctgagcgaga cgtcgctcag
     1801 ggcctgtacg ggcttgcctt gcgcgaacag gcggcgcggt ggcgcaaagc tcttgctgac
     1861 ggtctcgatg accagcatca cgcggctccc cgcgccggat cgagcctgat gcaggcggca
     1921 agatggcctg cgccaacagc cacgggcgcc ggtctggcgg cacggcaggc ggc
//
